Miracle-dz/UniFAR-FTUs huggingface dataset:
Root vacuolar Na+ sequestration but not exclusion from uptake correlates with barley salt tolerance.

Soil salinity is a major constraint for the global agricultural production. For many decades, Na+ exclusion from uptake has been the key trait targeted in breeding programs; yet, no major breakthrough in creating salt tolerant germplasm was achieved. In this work, we have combined the MIFE technique for non-invasive ion flux measurements with confocal fluorescence dye imaging technique to screen 45 accessions of barley to reveal the relative contribution of Na+ exclusion from the cytosol to the apoplast and its vacuolar sequestration in the root apex, for the overall salinity stress tolerance. We show that Na+ /H+ antiporter-mediated Na+ extrusion from the root plays a minor role in the overall salt tolerance in barley. At the same time, a strong and positive correlation was found between root vacuolar Na+ sequestration ability and the overall salt tolerance. The inability of salt sensitive genotypes to sequester Na+ in root vacuoles was in a contrast to a significantly higher expression levels of both HvNHX1 tonoplast Na+ /H+ antiporters and HvVP1 H+ -pumps compared with tolerant genotypes. This data is interpreted as a failure of sensitive varieties to prevent Na+ back-leak into the cytosol and existence of a futile Na+ cycle at the tonoplast. Taken together, our results demonstrated that root vacuolar Na+ sequestration but not exclusion from uptake played the main role in barley salinity tolerance and suggested that the focus of the breeding programs should be shifted from targeting genes mediating Na+ exclusion from uptake by roots to more efficient root vacuolar Na+ sequestration. This article is protected by copyright. All rights reserved.
Regulatory T Cells Thymus Exosomes-Like Particles Induce

Exosomes released from different types of cells have been proposed to contribute to intercellular communication. We report that thymic exosome-like particles (ELPs) released from cells of the thymus can induce the development of Foxp3 (cid:1) regulatory T (Treg) cells in the lung and liver. Thymic ELPs also induce the conversion of thymic CD4 (cid:1) CD25 (cid:2) T cells into Tregs. Tregs induced by thymic ELPs suppress the proliferation of CD4 (cid:1) CD25 (cid:2) T cells in vitro and in vivo. We further show that neutralization of TGF- (cid:3) in ELPs partially reverses thymic ELP-mediated induction of CD4 (cid:1) Foxp3 (cid:1) T cells in the lung and liver. This study demonstrates that thymic ELPs participate in the induction of Foxp3 (cid:1) Tregs. Also, TGF- (cid:3) of thymic ELPs might be required for the generation of Tregs in the peripheral tissues. The Journal of Immunology, 2008, 181: 5242–5248.
Respiratory virus infection triggers acute psoriasis flares across different clinical subtypes and genetic backgrounds

Psoriasis is a chronic inflammatory skin disease affecting 1-5% of the population worldwide.1 Its predominant plaque psoriasis/psoriasis vulgaris (PV) subtype results from interactions of complex genetic backgrounds with environmental factors, leading to dysregulated skin immune responses. This article is protected by copyright. All rights reserved.
Farmer perception of fall armyworm (Spodoptera frugiderda J.E. Smith) and farm‐level management practices in Zambia

Abstract BACKGROUND This paper documents farmer perceptions and management practices for fall armyworm (Spodoptera frugiderda J.E. Smith), providing a baseline for the development of sustainable pest management strategies. RESULTS 91% of farmers correctly identified fall armyworm, and reported it as the most important maize pest during 2016/2017 cropping season, affecting nearly half of cultivated area. Estimated maize yield loss during the season, attributed to fall armyworm was 28%. A majority of farmers (60%) used pesticides for fall armyworm control, along with other cultural/physical practices – hand picking and crushing egg masses/caterpillars (36%), and application of ash/sand in the funnel (19%). Farmers used various pesticide active ingredients, and protective measures were inadequate; >50% of farmers did not use any protective measures while spraying. Significantly more male than female farmers used pesticides (P = 0.05), and the reverse was true for cultural practices. Significant maize yield differences (P = 0.001) were observed by gender, attributed to differences in utilization of production inputs/practices. At least 77% of farmers received and shared agricultural advice, which can be optimized to spread information on fall armyworm management options. CONCLUSION Increased use of pesticides to manage fall armyworm poses health and environmental risks, besides the high cost for farmers and governments. Research into cultural and indigenous practices used by farmers will offer opportunities for alternative and sustainable management practices. Research efforts should pay attention to gender differences in access to resources and inputs. Tackling fall armyworm at the farm level, and averting yield losses will require integrated messaging addressing other production risks. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Differences in STI Risk comparing PrEP users and propensity score-matched historical controls in a clinic setting.

OBJECTIVE To determine whether men who have sex with men (MSM) using pre-exposure prophylaxis (PrEP) are at higher risk of bacterial sexually transmitted infections (STIs) compared to MSM not using PrEP. DESIGN Secondary analysis of longitudinal STI data obtained from MSM attending an STD Clinic in Seattle, WA, October 2011 - September 2017. METHODS We identified patients obtaining PrEP through the STD Clinic, and used propensity score matching to select a historical group of similar patients not using PrEP for comparison. We linked patient data with STI surveillance data to compare the incidence of chlamydia, gonorrhea, and early syphilis, and time to first symptomatic STI among PrEP users and non-users. RESULTS 365 PrEP users who picked up prescriptions and returned for follow-up and 730 propensity score-matched non-users were included in the analysis. Adjusted incidence rate ratios (aIRR) for chlamydia, gonorrhea, and early syphilis were 3.2 (95% confidence interval [CI]: 1.9 - 5.3), 2.8 (95% CI: 1.7 - 4.6), and 2.9 (95% CI: 1.5 - 5.6), respectively, comparing PrEP users to non-users. Time to first symptomatic STI was shorter among PrEP users (120 days, 95% CI: 77 - 171) compared to non-users (185 days, 95% CI: 163 - 256). CONCLUSIONS Among MSM on PrEP, we observed a higher incidence of STIs and faster time to first symptomatic STI compared to MSM not using PrEP. PrEP may be a contributing factor in increasing STI rates among MSM.
DIETARY PATTERNS AND THEIR ASSOCIATION WITH OBESITY: A CROSS-SECTIONAL STUDY.

Context Scarce data on dietary habits in Eastern European countries is available and reports investigated individual food items and not dietary patterns in these populations. Objective To identify dietary patterns and to explore their association with obesity in a sample from Romanian population. Design Cross-sectional. Subjects and Methods This was an analysis of data collected from 1398 adult participants in ORO study. Data on lifestyle, eating habits and food frequency consumption were collected. Results By principal component analysis we identified 3 dietary patterns explaining 31.4% of the diet variation: High meat/High fat pattern, Western pattern and Prudent pattern. High meat/High fat pattern was associated with male gender, lower educational level, living in a rural, smoking and a higher probability for the presence of obesity (OR 1.2 [95%CI: 1.1-1.4]). Western pattern was associated with younger age, a higher level of physical activity and smoking. Prudent pattern was associated with older age, female gender, a higher level of physical activity, not smoking status and a lower probability for the presence of obesity (OR 0.8 [95%CI: 0.7-0.9]). Conclusions This study provides for the first-time information on the association between dietary patterns in adults from an Eastern European country and the presence of obesity.
Body Mass Index and Associated Clinical Variables in Patients with Non-Celiac Wheat Sensitivity

Background: Non-Celiac Wheat Sensitivity (NCWS) is still a largely undefined condition, due to the lack of a diagnostic marker. Few data are available about the nutritional characteristics of NCWS patients at diagnosis. Aims: To evaluate the proportion of NCWS patients who were underweight, normal weight, overweight, or obese at diagnosis, and to search for possible correlations between their Body Mass Index (BMI) and other NCWS-related disease characteristics. Patients and Methods: The clinical charts of 145 NCWS patients (125 F, 20 M, mean age 37.1 ± 11.4 years), diagnosed between January 2012 and March 2018, were reviewed. As a comparison, 84 celiac disease (CD) patients (73 F, 11 M, mean age 39.8 ± 13.9 years) were evaluated. All NCWS diagnoses were based on a double-blind placebo-controlled wheat challenge (DBPCWC) method. Results: BMI distribution was similar in the NCWS (6.2% underweight and 15.2% obese subjects) and CD patients (6% underweight and 7.1% obese subjects). Underweight NCWS subjects were significantly younger and had a shorter clinical history than the overweight or obese ones. Unlike the other NCWS patients, none of them had a DQ2 and/or DQ8 haplotype. Overweight and obese NCWS patients were more frequently suffering from associated autoimmune diseases than the other BMI categories (P = 0.05). Compared to the CD controls, NCWS patients showed a higher frequency of Irritable Bowel Syndrome (IBS)-like (P = 0.01) and extraintestinal symptoms (P = 0.03) and a longer clinical history (P = 0.04), whereas weight loss was more frequent in CD (P = 0.02). Conclusions: NCWS patients showed a BMI distribution similar to CD patients. However, NCWS was found to be a heterogenous condition that regards BMI, and clinical characteristics differed between the underweight and overweight/obese patients.
Prediction of Tumor Control in 90Y Radioembolization by Logit Models with PET/CT-Based Dose Metrics

The aim of this work was to develop models for tumor control probability (TCP) in radioembolization with 90Y PET/CT–derived radiobiologic dose metrics. Methods: Patients with primary liver cancer or liver metastases who underwent radioembolization with glass microspheres were imaged with 90Y PET/CT for voxel-level dosimetry to determine lesion absorbed dose (AD) metrics, biological effective dose (BED) metrics, equivalent uniform dose, and equivalent uniform BED for 28 treatments (89 lesions). The lesion dose–shrinkage correlation was assessed on the basis of RECIST and, when available, modified RECIST (mRECIST) at first follow-up. For a subset with mRECIST, logit regression TCP models were fit via maximum likelihood to relate lesion-level binary response to the dose metrics. As an exploratory analysis, the nontumoral liver dose–toxicity relationship was also evaluated. Results: Lesion dose–shrinkage analysis showed that there were no significant differences between model parameters for primary and metastatic subgroups and that correlation coefficients were superior with mRECIST. Therefore, subsequent TCP analysis was performed for the combined group using mRECIST only. The overall lesion-level mRECIST response rate was 57%. The AD and BED metrics yielding 50% TCP were 292 and 441 Gy, respectively. All dose metrics considered for TCP modeling, including mean AD, were significantly associated with the probability of response, with high areas under the curve (0.87–0.90, P < 0.0001) and high sensitivity (>0.75) and specificity (>0.83) calculated using a threshold corresponding to 50% TCP. Because nonuniform AD deposition by microspheres cannot be determined by PET at a microscopic scale, radiosensitivity values extracted here by fitting models to clinical response data were substantially lower than reported for in vitro cell cultures or for external-beam radiotherapy clinical studies. There was no correlation between nontumoral liver AD and toxicity measures. Conclusion: Despite the heterogeneous patient cohort, logistic regression TCP models showed a strong association between various dose metrics and the probability of response. The performance of mean AD was comparable to that of radiobiologic dose metrics that involve more complex calculations. These results demonstrate the importance of considering TCP in treatment planning for radioembolization.
Disorder of Coagulation-Fibrinolysis System: An Emerging Toxicity of Anti-PD-1/PD-L1 Monoclonal Antibodies

A disruption of immune checkpoints leads to imbalances in immune homeostasis, resulting in immune-related adverse events. Recent case studies have suggested the association between immune checkpoint inhibitors (ICIs) and the disorders of the coagulation-fibrinolysis system, implying that systemic immune activation may impact a balance between clotting and bleeding. However, little is known about the association of coagulation-fibrinolysis system disorder with the efficacy of ICIs. We retrospectively evaluated 83 lung cancer patients who received ICI at Kumamoto University Hospital. The association between clinical outcome and diseases associated with disorders of the coagulation-fibrinolysis system was assessed along with tumor PD-L1 expression. Among 83 NSCLC patients, total 10 patients (12%) developed diseases associated with the disorder of coagulation-fibrinolysis system. We found that disorders of the coagulation-fibrinolysis system occurred in patients with high PD-L1 expression and in the early period of ICI initiation. In addition, high tumor responses (72%) were observed, including two complete responses among these patients. Furthermore, we demonstrate T-cell activation strongly induces production of a primary initiator of coagulation, tissue factor in peripheral PD-L1high monocytes, in vitro. This study suggests a previously unrecognized pivotal role for immune activation in triggering disorders of the coagulation-fibrinolysis system in cancer patients during treatment with ICI.
Association between advanced cancer patient‐caregiver agreement regarding prognosis and hospice enrollment

Patients with advanced, incurable cancer who understand their illness is incurable are more likely to prefer hospice care at the end of life compared with patients who believe their illness is curable. To the authors' knowledge, it is unclear whether patient‐caregiver agreement regarding perceived prognosis is associated with hospice enrollment.
Surface Energy of Filtration Media Influencing the Filtration Performance against Solid Particles, Oily Aerosol, and Bacterial Aerosol

Particulate airborne pollutants are a big concern to public health, and it brings growing attention about effective filtration devices. Especially, particulate matters smaller than 2.5 µm can reach the thoracic region and the blood stream, and the associated health risk can be exacerbated when pathogenic microbials are present in the air. This study aims at understanding the surface characteristics of nonwoven media that influence filtration performance against solid particles (sodium chloride, NaCl), oily aerosol (dioctyl phthalate, DOP), and Staphylococcus aureus (S. aureus) bacteria. Nonwoven media of polystyrene (PS) fibers were fabricated by electrospinning and its pristine surface energy (38.5 mN/m) was modified to decrease (12.3 mN/m) by the plasma enhanced chemical vapor deposition (PECVD) of octafluorocyclobutane (C4F8) or to increase (68.5 mN/m) by the oxygen (O2) plasma treatment. For NaCl particles and S. aureus aerosol, PS electrospun web showed higher quality factor than polypropylene (PP) meltblown electret that is readily available for commercial products. The O2 plasma treatment of PS media significantly deteriorated the filtration efficiency, presumably due to the quick dissipation of static charges by the O2 plasma treatment. The C4F8 treated, fluorinated PS media resisted quick wetting of DOP, and its filtration efficiency for DOP and S. aureus remained similar while its efficiency for NaCl decreased. The findings of this study will impact on determining relevant surface treatments for effective particulate filtration. As this study examined the instantaneous performance within 1–2 min of particulate exposure, and the further study with the extended exposure is suggested.
The salivary transcriptome of Limnobdella mexicana (Annelida: Clitellata: Praobdellidae) and orthology determination of major leech anticoagulants

Abstract Bloodfeeding requires several adaptations that allow the parasite to feed efficiently. Leeches and other hematophagous animals have developed different mechanisms to inhibit hemostasis, one of the main barriers imposed by their hosts. Limnobdella mexicana is a member of the leech family Praobdellidae, a family of host generalists known for their preference to attach on mucosal membranes of mammals, such as those in nasopharyngeal cavities, bladders and ocular orbits. Previous studies have hypothesized a positive relationship between diversity of anticoagulants and diversity of hosts in bloodfeeding leeches. However, orthology determination of putative anticoagulants and the lack of standardization of sequencing effort and method hinder comparisons between publicly available transcriptomes generated in different laboratories. In the present study, we examine the first transcriptome of a praobdellid leech and identify 15 putative anticoagulants using a phylogeny-based inference approach, amino-acid conservation, Pfam domains and BLAST searches. Our phylogenetic analyses suggest that the ancestral leech was able to inhibit factor Xa and that some hirudins that have been reported in previous studies on leech anticoagulants may not be orthologous with the archetypal hirudin.
Six weeks of training does not change running mechanics or improve running economy.

Running technique and economy (VO2submax) were examined before and after a 6-wk period of running training. Fifteen males were filmed and performed 10-min economy runs at 3.36 m.s-1 on a treadmill. An incremental treadmill test was used to record running performance and maximal oxygen consumption (VO2max). Subjects were randomly assigned to a training group and a control group that did not participate in any running program. There were no significant changes in kinematic variables between pre- and post-training tests for either group. Neither were there any significant physiological changes over the 6 wk in the control group. However, the training group demonstrated a significantly (P < 0.01) increased VO2max (57.7 +/- 6.2 vs 61.3 +/- 6.3 ml.kg-1.min-1) and running performance. VO2submax in the training group was significantly (P < 0.05) worse (41.0 +/- 4.5 vs 42.4 +/- 4.3 ml.kg-1.min-1) post-training, although the percent utilization of VO2max (71.6 +/- 7.9 vs 69.3 +/- 6.9%) and submaximal heart rate (169 +/- 15 vs 161 +/- 15 beats.min-1) were significantly lower (P < 0.05). The training-induced improvements in running performance could be attributed to physiological rather than biomechanical modifications. There were no changes in biomechanical descriptors of running style that signaled changes in running economy.
Nanoparticle Characterization: What to Measure?

What to measure? is a key question in nanoscience, and it is not straightforward to address as different physicochemical properties define a nanoparticle sample. Most prominent among these properties are size, shape, surface charge, and porosity. Today researchers have an unprecedented variety of measurement techniques at their disposal to assign precise numerical values to those parameters. However, methods based on different physical principles probe different aspects, not only of the particles themselves, but also of their preparation history and their environment at the time of measurement. Understanding these connections can be of great value for interpreting characterization results and ultimately controlling the nanoparticle structure–function relationship. Here, the current techniques that enable the precise measurement of these fundamental nanoparticle properties are presented and their practical advantages and disadvantages are discussed. Some recommendations of how the physicochemical parameters of nanoparticles should be investigated and how to fully characterize these properties in different environments according to the intended nanoparticle use are proposed. The intention is to improve comparability of nanoparticle properties and performance to ensure the successful transfer of scientific knowledge to industrial real‐world applications.
The mysterious presence of a 5-methylcytosine oxidase in the Drosophila genome

5-methylcytosine is an important epigenetic modification involved in gene control in vertebrates and many other complex living organisms. Its presence in Drosophila has been a matter of debate and recent bisulfite sequencing studies of early-stage fly embryos have concluded that the genome of Drosophila is essentially unmethylated. However, as we outline here, the Drosophila genome harbors a well-conserved homolog of the TET protein family. The mammalian orthologs TET1/2/3 are known to convert 5-methylcytosine into 5-hydroxymethylcytosine. We discuss several possible explanations for these seemingly contradictory findings. One possibility is that the 2 modified cytosine bases are generated in Drosophila only at certain developmental stages and in a cell type-specific manner during neurogenesis. Alternatively, Drosophila Tet and its mammalian homologs may carry out catalytic activity-independent functions, and the possibility that these proteins may oxidize 5-methylcytosine in RNA created by the methyltransferase Dnmt2 should also be strongly considered.
Cost‐effectiveness of sunflower oil fortification with vitamin A in Tanzania by scale

Abstract In 2011, Tanzania mandated the fortification of edible oil with vitamin A to help address its vitamin A deficiency (VAD) public health problem. By 2015, only 16% of edible oil met the standards for adequate fortification. There is no evidence on the cost‐effectiveness of the fortification of edible oil by small‐ and medium‐scale (SMS) producers in preventing VAD. The MASAVA project initiated the production of sunflower oil fortified with vitamin A by SMS producers in the Manyara and Shinyanga regions of Tanzania. A quasi‐experimental nonequivalent control‐group research trial and an economic evaluation were conducted. The household survey included mother and child pairs from a sample of 568 households before the intervention and 18 months later. From the social perspective, the incremental cost of fortification of sunflower oil could be as low as $0.13, $0.06, and $0.02 per litre for small‐, medium‐, and large‐scale producers, respectively, compared with unfortified sunflower oil. The SMS intervention increased access to fortified oil for some vulnerable groups but did not have a significant effect on the prevention of VAD due to insufficient coverage. Fortification of vegetable oil by large‐scale producers was associated with a significant reduction of VAD in children from Shinyanga. The estimated cost per disability‐adjusted life year averted for fortified sunflower oil was $281 for large‐scale and could be as low as $626 for medium‐scale and $1,507 for small‐scale producers under ideal conditions. According to the World Health Organization thresholds, this intervention is very cost‐effective for large‐ and medium‐scale producers and cost‐effective for small‐scale producers.