Miracle-dz/UniFAR-FTUs huggingface dataset:
ASN1‐encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds

Despite a general view that asparagine synthetase generates asparagine as an amino acid for long-distance transport of nitrogen to sink organs, its role in nitrogen metabolic pathways in floral organs during seed nitrogen filling has remained undefined. We demonstrate that the onset of pollination in Arabidopsis induces selected genes for asparagine metabolism, namely ASN1 (At3g47340), GLN2 (At5g35630), GLU1 (At5g04140), AapAT2 (At5g19950), ASPGA1 (At5g08100) and ASPGB1 (At3g16150), particularly at the ovule stage (stage 0), accompanied by enhanced asparagine synthetase protein, asparagine and total amino acids. Immunolocalization confined asparagine synthetase to the vascular cells of the silique cell wall and septum, but also to the outer and inner seed integuments, demonstrating the post-phloem transport of asparagine in these cells to developing embryos. In the asn1 mutant, aberrant embryo cell divisions in upper suspensor cell layers from globular to heart stages assign a role for nitrogen in differentiating embryos within the ovary. Induction of asparagine metabolic genes by light/dark and nitrate supports fine shifts of nitrogen metabolic pathways. In transgenic Arabidopsis expressing promoterCaMV35S ::ASN1 fusion, marked metabolomics changes at stage 0, including a several-fold increase in free asparagine, are correlated to enhanced seed nitrogen. However, specific promoterNapin2S ::ASN1 expression during seed formation and a six-fold increase in asparagine toward the desiccation stage result in wild-type seed nitrogen, underlining that delayed accumulation of asparagine impairs the timing of its use by releasing amide and amino nitrogen. Transcript and metabolite profiles in floral organs match the carbon and nitrogen partitioning to generate energy via the tricarboxylic acid cycle, GABA shunt and phosphorylated serine synthetic pathway.
INVESTMENT, INSURANCE AND WEATHER SHOCKS:EVIDENCE FROM A LAB EXPERIMENT IN CAMBODIA

The paper studies the relationships between investment and insurance under risk and ambiguity. It makes use of a lab experiment to show that under ambiguity poor farmers experience a substitution effect between in- vestment and insurance. Moreover, although having the possibility to purchase an insurance increases, overall, the investment in profitable but risky inputs, this effect is heterogeneous across wealth and uncertainty status. Subsidies positively affect insurance purchase, negatively affect investment in risky inputs and reduce the substitution effect experienced by poor farmers. Finally, the paper shows that under ambiguity people rely on heuristic tools.
Development of a cytokine analog with enhanced stability using computational ultrahigh throughput screening

Granulocyte‐colony stimulating factor (G‐CSF) is used worldwide to prevent neutropenia caused by high‐dose chemotherapy. It has limited stability, strict formulation and storage requirements, and because of poor oral absorption must be administered by injection (typically daily). Thus, there is significant interest in developing analogs with improved pharmacological properties. We used our ultrahigh throughput computational screening method to improve the physicochemical characteristics of G‐CSF. Improving these properties can make a molecule more robust, enhance its shelf life, or make it more amenable to alternate delivery systems and formulations. It can also affect clinically important features such as pharmacokinetics. Residues in the buried core were selected for optimization to minimize changes to the surface, thereby maintaining the active site and limiting the designed protein's potential for antigenicity. Using a structure that was homology modeled from bovine G‐CSF, core designs of 25–34 residues were completed, corresponding to 1021–1028 sequences screened. The optimal sequence from each design was selected for biophysical characterization and experimental testing; each had 10–14 mutations. The designed proteins showed enhanced thermal stabilities of up to 13°C, displayed five‐to 10‐fold improvements in shelf life, and were biologically active in cell proliferation assays and in a neutropenic mouse model. Pharmacokinetic studies in monkeys showed that subcutaneous injection of the designed analogs results in greater systemic exposure, probably attributable to improved absorption from the subcutaneous compartment. These results show that our computational method can be used to develop improved pharmaceuticals and illustrate its utility as a powerful protein design tool.
Inhibition of human cytomegalovirus in culture by alkenyl guanine analogs of the thiazolo[4,5-d]pyrimidine ring system

A series of alkyl and alkenyl guanine analogs containing a thiazolo[4,5-d]pyrimidine ring system were prepared by reaction of the appropriate alkyl halide with the sodium salt of the heterocycle. In preliminary antiviral efficacy evaluations against laboratory strains of both human cytomegalovirus (HCMV) and herpes simplex virus types 1 and 2, it was determined that two of the compounds (T70072 and T01132) were more active and less toxic in stationary-phase cell monolayers than were the other derivatives tested. T01132 and T70072, which have 2-pentenyl and 3-methyl-2-butenyl moieties attached to position 3 of the 5-aminothiazolo[4,5-d]pyrimidine-2,7-dione, respectively, were then more extensively evaluated for anti-HCMV activity. The concentrations of T01132 and T70072 required to inhibit HCMV by 50% in plaque reduction assays were approximately 0.5 and 6.8 microM, respectively. These two compounds inhibited the growth of KB, MRC-5, or Vero cells at concentrations of 75 to 150 microM, depending upon the cell line. In bone marrow progenitor cells T01132 was slightly less toxic than ganciclovir (DHPG). The 50% inhibitory concentrations of T01132 against clinical isolates and DHPG-resistant strains of HCMV were approximately the same as those obtained for laboratory strains of HCMV (approximately 0.5 microM). When tested in combination with DHPG, the resultant antiviral activity was determined to be additive but not synergistic. Experiments performed using variations of the viral multiplicity of infection (MOI) demonstrated that T01132 was more active than DHPG at a low MOI (0.002 or 0.02). However, when a higher MOI (0.2 or 2.0) was used, DHPG was more efficacious than T01132. In experiments in which drug was added at various times post-viral infection, T01132 was most effective when added within the first 24 h post-HCMV infection while DHPG was able to protect cells in this assay system when added up to 48 h postinfection, indicating that T01132 is exerting its antiviral effect on events leading up to and possibly including viral DNA synthesis. The data presented in this report suggest that the antiviral activity of alkenyl-substituted thiazolopyrimidine derivatives may represent a mechanism of action against herpesviruses alternative to that of classical nucleoside analogs such as acyclovir or DHPG.
Transient Receptor Potential Canonical 3 (TRPC3) Channels Are Required for Hypothalamic Glucose Detection and Energy Homeostasis

The mediobasal hypothalamus (MBH) contains neurons capable of directly detecting metabolic signals such as glucose to control energy homeostasis. Among them, glucose-excited (GE) neurons increase their electrical activity when glucose rises. In view of previous work, we hypothesized that transient receptor potential canonical type 3 (TRPC3) channels are involved in hypothalamic glucose detection and the control of energy homeostasis. To investigate the role of TRPC3, we used constitutive and conditional TRPC3-deficient mouse models. Hypothalamic glucose detection was studied in vivo by measuring food intake and insulin secretion in response to increased brain glucose level. The role of TRPC3 in GE neuron response to glucose was studied by using in vitro calcium imaging on freshly dissociated MBH neurons. We found that whole-body and MBH TRPC3-deficient mice have increased body weight and food intake. The anorectic effect of intracerebroventricular glucose and the insulin secretory response to intracarotid glucose injection are blunted in TRPC3-deficient mice. TRPC3 loss of function or pharmacological inhibition blunts calcium responses to glucose in MBH neurons in vitro. Together, the results demonstrate that TRPC3 channels are required for the response to glucose of MBH GE neurons and the central effect of glucose on insulin secretion and food intake.
Pesticidal Evaluation of Manilkara zapota (L.) against Tribolium castaneum (Herbst)

This paper presents the residual eff ects of Manilkara zapota (L.) P. Royen plant against the red flour beetle, Tribolium castaneum (Herbst). Four doses, i.e., 1238.5, 619.25, 309.6 and 154.8 μg/cm2 of ethyl acetate extract of stem bark of Manilkara zapota were applied on larvae and adult beetles. The effectiveness of the plant extract was increased with the increase of exposure time and after 72 hrs of exposure, the maximum residual toxicity was observed with LD50 of 228.8, 281.1, 413.4, 423.7, 455.2, 498.7 and 526.5 μg/cm2 for first, second, third, fourth, fifth, sixth instar larvae and adults, respectively. The results of this study also demonstrated that the earlier instars were more sensitive to the extracts than those of late instars as well as adults.
Agronomic and physiological aspects of nitrogen use efficiency in conventional and organic cereal-based production systems

Abstract Better management of synthetic nitrogen (N) fertilizers in conventional agricultural systems laid the foundation for feeding the increasing world's population since the Green Revolution. However, excessive reliance on inorganic fertilizer has resulted in environmental degradation issues. Difficulties in soil nutrition management in organic cropping systems often results in lower and variable yields, also raising questions of sustainability. Improving nitrogen use efficiency (NUE) is thus of key importance to overcome environmental concerns in conventional systems and production limitations in organic systems. The differences in the two farming systems have impacts on crop traits and N cycles, making it difficult to enhance NUE with a single strategy. Different approaches need to be adopted to improve NUE in each system. Extensive efforts have been made to better understand mechanisms to potentially improve NUE in cereal crops under both systems. This review suggests that NUE may be improved through a combination of management practices and breeding strategies specific to the management system. Diversified crop rotations with legumes are effective practices to optimize the N cycle in both conventional and organic systems. Best Management Practices coupled with nitrification inhibitors, controlled release products and split-application practices can reduce N loss in conventional systems. In organic systems, we need to take advantage of available N sources and adapt practices such as no-tillage, cover crops, and catch crops. Utilization of beneficial soil microorganisms is fundamental to optimizing availability of soil N. Estimation of soil organic matter mineralization using prediction models may be useful to enhance NUE if models are calibrated for target environments. Cereal crops are often bred under optimum N conditions and may not perform well under low N conditions. Thus, breeders can integrate genetic and phenotypic information to develop cultivars adapted to specific environments and cultivation practices. The proper choice and integration of strategies can synchronize N demand and supply within a system, resulting in reduced risk of N loss while improving NUE in both conventional and organic systems.
Research proposal: effectiveness of curriculum activity towards the establishment of identity of students of higher education institutions in the present

Ministry of Education (MOE) outlines the core curriculum for students who are still studying in Institutions of Higher Education (MOHE) effectiveness in shaping the identity of students, including studies that have been done in connection with extra-curricular activities. Extra-curricular activities can indirectly increase the level of self-esteem tertiary students nowadays for playing an important role in education. To foster the formation of personality and unity. As the younger generation and people in this country in the curriculum can instil feelings of goodwill, unity, national integration and inculcating good values while participating in these activities. In accordance with the core curriculum to develop human capital to establish the effectiveness of the self-esteem of students. This study shows that extra-curricular activities provide efficacy against identity university students nowadays.
Simulating Enhanced Meshes, with Applications

The purpose of this paper is to present non-trivial lower bounds for two classes of enhanced meshes. To achieve this goal we present efficient simulations of these two classes of enhanced meshes on the CREW-PRAM and Common-CRCW. From this, known computational lower bounds for the PRAM are transferred to the enhanced meshes. Our approach of transferring lower bounds from the PRAM to enhanced meshes is novel. Specifically, we show that any computation that takes O(t(n)) steps on an n-processor mesh with multiple broadcasting can be performed in O(t(n)) steps on an n-processor CREW-PRAM with O(n) extra memory. Similarly, with α standing for the inverse Ackermann function, we show that any computation that takes O(t(n)) computational steps on an n-processor basic reconfigurable mesh can be performed in O(α(n)t(n)) computational steps on an n-processor Common-CRCW with O(n) extra memory. We also show that some of the lower bounds that we derive are tight by providing matching upper bounds; as an example, we sh.
Particulate Air Pollution and Chronic Respiratory Symptoms among Traffic Policemen in Bangkok

In this cross-sectional study, the authors examined the relationship between traffic-based air pollution and chronic, nonspecific respiratory symptoms among traffic policemen in Bangkok, Thailand. A total of 1,603 policemen who lived and worked in areas that had 3 different levels of airborne particulates were evaluated. The authors used a modified standardized questionnaire to identify nonspecific respiratory disease (NSRD) in participants. The prevalence of NSRD in heavily polluted, moderately polluted, and suburban areas was 13.0%, 10.9%, and 9.4%, respectively. Among nonsmokers, the age-adjusted prevalence of NSRD in the heavily polluted areas was significantly higher than in the suburban control area. Also among nonsmokers, the odds ratio for NSRD for each 10-μg/m3 increase in ambient particulate matter was 1.11. The authors concluded that the increased prevalence of respiratory symptoms among traffic policemen in Bangkok was associated with urban traffic air pollution.
LUNG CARCINOID WITH CUSHING'S SYNDROME: CONTROL OF SERUM ACTH AND CORTISOL LEVELS USING SMS 201–995 (SANDOSTATIN)

Two patients with Cushing's syndrome due to lung carcinoid tumours were given the long‐acting somatostatin analogue SMS 201–995 (Sandostatin). One received a single 50 μg dose which produced a 50% reduction in circulating ACTH levels within 4 h. The other has been maintained in clinical and biochemical remission for 10 weeks on 100 μg tid. This is the first report of the successful use of SMS 201–995 in carcinoid‐induced Cushing's syndrome, and suggests that this hormone analogue could be valuable in the long term medical management of such patients.
Do Multi-Currency Price Tags Bias Price Perceptions?

Stefan Thomas, Research Associate at the Chair of Marketing, University of Augsburg, Universitätsstrasse 16, 86159 Augsburg, Germany, phone: 0049 821 598 4048, E-Mail: stefan.thomas@ wiwi.uni-augsburg.de. Heribert Gierl, Professor of Marketing, University of Augsburg, Universitätsstrasse 16, 86159 Augsburg, Germany, phone: 0049 821 598 4052, E-Mail: heribert.gierl@wiwi.uniaugsburg.de. * Corresponding author Do Multi-Currency Price Tags Bias Price Perceptions?
Community perception of childhood drowning and its prevention measures in rural Bangladesh: a qualitative study.

OBJECTIVES To gain an in-depth understanding of people's perception of causes and their concepts of prevention of childhood drowning in rural Bangladesh. DESIGN A qualitative study and focus group discussion (FGD) was adopted. SETTING A rural community in Bangladesh. PARTICIPANTS FGDs were conducted with mothers of children aged under 5 years, adolescent male and female students, fathers and local leaders. One FGD was conducted for each group. Out of 53 participants 25 were women. RESULTS The respondents considered that children of 5-10 years are at risk of drowning. Ponds, ditches and canals were frequently mentioned locations of drowning. Most of the drownings were reported to occur around noon. For prevention of childhood drowning the participants suggested that the children should be constantly supervised, unwanted ditches should be filled in, ponds should be fenced and drowning prevention awareness in the community be increased by community leaders. They suggested that government should organise campaigns for preventing childhood drowning, promoting swimming instruction activities for children and motivating communities to fence ponds. CONCLUSIONS People interviewed in general know the causes of childhood drowning and its preventive measures, but they do not put their knowledge into preventative actions as they fail to recognise this as a major child survival issue and they are never reached with definite actions points to change the behaviours.
Pathogenomic Sequence Analysis of Bacillus cereus and Bacillus thuringiensis Isolates Closely Related to Bacillus anthracis

ABSTRACT Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of B. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.
Baldness and scalp melanoma

Nearly 20% of melanomas occur on the head and neck area1 but only 2-5% arise on the scalp.2 Several studies reported a worse prognosis of scalp melanoma compared to other body sites, being unclear the reason for this phenomenon.3 Melanoma and non-melanoma skin cancer of the scalp are most frequent in bald men older than 65 years. This article is protected by copyright. All rights reserved.
Heavy metals in rice samples on the Torbat-Heidarieh market, Iran

ABSTRACT Levels of the heavy metals arsenic (As), cadmium (Cd) and lead (Pb) were determined in rice samples imported from India and Pakistan and marketed in Torbat-Heidarieh, Iran. To assess the safety of dietary intake of these metals by rice, the estimated weekly intake (EWI) was calculated and compared with the provisional tolerable weekly intake (PTWI). The results indicated that mean values for Pb and cadmium in imported rice were considerably higher than the safe limit as set by FAO/WHO. EWI for cadmium and As among the consumed types of imported rice was lower than the PTWI. The results also revealed that the weekly intake of Pb was higher than the maximum weekly intake recommended by WHO/FAO.
Effect of eccentric versus concentric exercise training on mitochondrial function

Introduction: The effect of eccentric (ECC) versus concentric (CON) training on metabolic properties in skeletal muscle is understood poorly. We determined the responses in oxidative capacity and mitochondrial H2O2 production after eccentric (ECC) versus concentric (CON) training performed at similar mechanical power. Methods: Forty‐eight rats performed 5‐ or 20‐day eccentric (ECC) or concentric (CON) training programs. Mitochondrial respiration, H2O2 production, citrate synthase activity (CS), and skeletal muscle damage were assessed in gastrocnemius (GAS), soleus (SOL) and vastus intermedius (VI) muscles. Results: Maximal mitochondrial respiration improved only after 20 days of concentric (CON) training in GAS and SOL. H2O2 production increased specifically after 20 days of eccentric ECC training in VI. Skeletal muscle damage occurred transiently in VI after 5 days of ECC training. Conclusions: Twenty days of ECC versus CON training performed at similar mechanical power output do not increase skeletal muscle oxidative capacities, but it elevates mitochondrial H2O2 production in VI, presumably linked to transient muscle damage. Muscle Nerve 50: 803–811, 2014
Current practice compared with the international guidelines: endoscopic surveillance of Barrett's esophagus.

RATIONALE, AIMS AND OBJECTIVES To describe the current practice for the surveillance of patients with Barrett's esophagus, to compare this practice with the national guidelines published by the French Society of Digestive Endoscopy in 1998 and to identify the factors associated with the compliance to guidelines. METHOD To determine the attitudes of French hepatogastroenterologists to screening for Barrett's oesophagus, a postal anonymous questionnaire survey was undertaken. It was sent to 246 hepatogastroenterologists in the Rhone-Alpes area. We defined eight criteria allowed to assess the conformity of practices with the guidelines. We created three topics composed of several criterion. The topics analysed were 'Biopsies', 'Surveillance' and the diagnosis of high grade dysplasia. We studied the factors which could be associated with the compliance with the guidelines. RESULTS The response rate was 81.3%. For 58.0% of the gastroenterologists, endoscopic biopsy sampling were made according to French guidelines (four-quadrant biopsies at 2 cm intervals). Agreement was 78.0% regarding the interval of surveillance for no dysplasia (every 2 or 3 years) and 78.5% regarding the low-grade dysplasia (every 6 or 12 months). For the management of high-grade dysplasia, 28.6% actually confirm the diagnosis by a second anatomopathologist and 42.0% treated by proton pump inhibitor during 2 months. Concerning the biopsies, the young gastroenterologists and gastroenterologists practising in university hospitals had a better adherence to the guidelines (Relative Risk: 2.22, 95% CI 1.25-3.95 and 3.74, 95% CI 1.04-13.47, respectively). The other factors of risk were not statistically significant. CONCLUSIONS The endoscopic follow-up is mostly realized in accordance with the national guidelines. However, there is a wide variability in individual current practice.