Miracle-dz/UniFAR-FTUs huggingface dataset:
A Mercury-Catalyzed, High-Yield System for the Oxidation of Methane to Methanol

A homogeneous system for the selective, catalytic oxidation of methane to methanol via methyl bisulfate is reported. The net reaction catalyzed by mercuric ions, Hg(II), is the oxidation of methane by concentrated sulfuric acid to produce methyl bisulfate, water, and sulfur dioxide. The reaction is efficient. At a methane conversion of 50 percent, 85 percent selectivity to methyl bisulfate (∼43 percent yield; the major side product is carbon dioxide) was achieved at a molar productivity of 10–7 mole per cubic centimeter per second and Hg(II) turnover frequency of 10–3 per second. Separate hydrolysis of methyl bisulfate and reoxidation of the sulfur dioxide with air provides a potentially practical scheme for the oxidation of methane to methanol with molecular oxygen. The primary steps of the Hg(II)-catalyzed reaction were individually examined and the essential elements of the mechanism were identified. The Hg(II) ion reacts with methane by an electrophilic displacement mechanism to produce an observable species, CH3HgOSO3H, 1. Under the reaction conditions, 1 readily decomposes to CH3OSO3H and the reduced mercurous species, Hg22+ The catalytic cycle is completed by the reoxidation of Hg22+ with H2SO4 to regenerate Hg(II) and byproducts SO2 and H2O. Thallium(III), palladium(II), and the cations of platinum and gold also oxidize methane to methyl bisulfate in sulfuric acid.
Recurrence after resection with curative intent for distal cholangiocarcinoma

Few studies have been conducted on patterns of recurrence after resection for distal cholangiocarcinoma (DCC). The aim of this study was to investigate the incidence and pattern of recurrence after resection of DCC, and to evaluate prognostic factors for time to recurrence and recurrence‐free survival (RFS).
A Single-Boundary Accumulator Model of Response Times in an Addition Verification Task

Current theories of mathematical cognition offer competing accounts of the interplay between encoding and calculation in mental arithmetic. Additive models propose that manipulations of problem format do not interact with the cognitive processes used in calculation. Alternatively, interactive models suppose that format manipulations have a direct effect on calculation processes. In the present study, we tested these competing models by fitting participants' RT distributions in an arithmetic verification task with a single-boundary accumulator model (the shifted Wald distribution). We found that in addition to providing a more complete description of RT distributions, the accumulator model afforded a potentially more sensitive test of format effects. Specifically, we found that format affected drift rate, which implies that problem format has a direct impact on calculation processes. These data give further support for an interactive model of mental arithmetic.
Cigarette smoking is negatively associated with keratoconus.

PURPOSE To investigate a correlation between cigarette smoking and keratoconus. METHODS Patients with keratoconus who were treated with corneal collagen cross-linking from June 2006 to November 2007 were asked about their smoking habits. A person smoking a minimum of two cigarettes per day for more than 1 year was classified as a smoker. RESULTS A total of 180 patients with keratoconus (mean age 28 +/- 9 years [range: 15 to 41 years]) were asked about their smoking habits. One hundred seventy-one (95%) were non-smokers and only 9 (5%) were smokers (95% confidence interval, 2.31 to 9.28). Using the chi-square test, a significant correlation was found between non-smokers and keratoconus (P < .001). CONCLUSIONS In this group of patients with keratoconus, few were smokers. Cigarette smoke contains toxic substances. Consequently, people are advised not to smoke. However, we speculate that the by-products of cigarette smoke may lead to cross-linking of collagen, which in the cornea, may prevent the development and progression of keratoconus.
Intracortical connections and their physiological correlates in the primary auditory cortex (AI) of the cat

We studied the functional and anatomical properties of the intrinsic connections in the primary auditory cortex (AI) of the cat by using physiological mapping and retrograde tracing methods. Our results revealed that a focal micro inject ion of tracer labeled as many as five intracortical patches in AI. The patches contained labeled pyramidal and non‐pyramidal cell types, most of which were clustered in the middle layers. A densely distributed anterograde‐like reaction product was present in the superficial layers above the labeled cells. The distribution of the patches was anisotropic, with most patches occurring dorsal, ventral, and anterior to the injection site. We examined the correlation between the characteristic frequency (CF) and binaural response properties of the injected and labeled regions. We found local labeling in regions possessing CFs equivalent to or slightly greater than that of the injected area. This appears to be a specific connection since we were able to predict the general location of many of the patches on the basis of the organization of the isofrequency domains. Patches were more numerous dorsoposterior to the injection site when the isofrequency contours ran obliquely (i.e., dorsoposterior to ventroanterior) across AI. The binaural response properties of the injected and labelled regions, however, were unrelated.
ASSESSMENT OF PATIENTS' KNOWLEDGE ABOUT THEIR ILLNESS AND TREATMENT

One hundred and twenty two patients over the age of 65 were questioned about their knowledge of their illness and treatment. They were unaware of a quarter of both their illnesses and medications. There was a considerable lack of knowledge about certain groups of diseases and medications, while patients were much better informed about others. The possible reasons for this discrepancy are briefly discussed and some practical suggestions are put forward.
Neonatal Mice Lacking Neuronal Nitric Oxide Synthase Are Less Vulnerable to Hypoxic–Ischemic Injury

We hypothesized that elimination of neuronal nitric oxide synthase (nNOS) by targeted disruption of the nNOS gene would result in amelioration of damage seen after hypoxia-ischemia in the developing brain since nitric oxide (NO) has been implicated in glutamate-mediated neurotoxicity after ischemia. Both wildtype and nNOS-deficient pups were subjected to focal ischemia followed by 1.5 h of hypoxia at Postnatal Day 7. Seven days later, brains of surviving animals were analyzed for damage. The nNOS-deficient pups (n = 17) had less histopathologic evidence of injury in both the hippocampus (P = 0.008) and the cortex (P = 0.0008) than the wildtype (n = 30) mice. When injured, the nNOS-deficient mice had damage that was limited to the hippocampus. These results support a role for neuronally produced NO in injury after perinatal hypoxia-ischemia.
Immunohistochemical Localization of Carboxypeptidases E and D in the Human Placenta and Umbilical Cord

Carboxypeptidase E (CPE) is highly concentrated in neuroendocrine tissues and is the only carboxypeptidase detected in mature secretory vesicles. Carboxypeptidase D (CPD), a carboxypeptidase with CPE-like activity, is widely distributed in tissues and is present in the trans-Golgi network. Previous work had shown that both CPE and CPD are expressed in the human placenta and that CPD is expressed at much higher levels than CPE. The present work provides evidence for the co-localization of CPE and CPD to basal plate extravillous trophoblasts and maternal uteroplacental vascular endothelial cells, chorionic villous endothelial cells, amnionic epithelial cells, and umbilical venous and arterial smooth muscle cells. Whereas the intensity of CPD immunostaining is similar in the placenta and umbilical cord, CPE staining in the placenta is much weaker than in the umbilical cord, suggesting that CPD plays a more important role in the processing of placental peptides. Immunoelectron microscopy of umbilical venous smooth muscle cells shows subcellular localization of both enzymes to the rough endoplasmic reticulum. In addition, CPE is present just subjacent to the cell membrane. The difference in cellular and subcellular localization between the two enzymes indicates that they perform distinct functions in the processing of placental peptides and proteins.
Natural Infection of a Household Pet Red-Capped Mangabey (Cercocebus torquatus torquatus) with a New Simian Immunodeficiency Virus

ABSTRACT A seroprevalence survey was conducted for simian immunodeficiency virus (SIV) antibody in household pet monkeys in Gabon. Twenty-nine monkeys representing seven species were analyzed. By using human immunodeficiency virus type 2 (HIV-2)/SIVsm, SIVmnd, and SIVagm antigens, one red-capped mangabey (RCM) (Cercocebus torquatus torquatus) was identified as harboring SIV-cross-reactive antibodies. A virus isolate, termed SIVrcm, was subsequently established from this seropositive RCM by cocultivation of its peripheral blood mononuclear cells (PBMC) with PBMC from seronegative humans or RCMs. SIVrcm was also isolated by cocultivation of CD8-depleted RCM PBMC with Molt 4 clone 8 cells but not with CEMx174 cells. The lack of growth in CEMx174 cells distinguished this new SIV from all previously reported sooty mangabey-derived viruses (SIVsm), which grow well in this cell line. SIVrcm was also successfully transmitted (cell free) to human and rhesus PBMC as well as to Molt 4 clone 8 cells. To determine the evolutionary origins of this newly identified virus, subgenomic pol (475 bp) andgag (954 bp) gene fragments were amplified from infected cell culture DNA and sequenced. The position of SIVrcm relative to those of members of the other primate lentivirus lineages was then examined in evolutionary trees constructed from deduced protein sequences. This analysis revealed significantly discordant phylogenetic positions of SIVrcm in the two genomic regions. In trees derived from partial gag sequences, SIVrcm clustered independently from all other HIV and SIV strains, consistent with a new primate lentivirus lineage. However, in trees derived frompol sequences, SIVrcm grouped with the HIV-1/SIVcpz lineage. These findings suggest that the SIVrcm genome is mosaic and possibly is the result of a recombination event involving divergent lentiviruses in the distant past. Further analysis of this and other SIVrcm isolates may shed new light on the origin of HIV-1.
Implementation of a targeted screening program to detect airflow obstruction suggestive of chronic obstructive pulmonary disease within a presurgical screening clinic.

BACKGROUND Targeted spirometry screening for chronic obstructive pulmonary disease (COPD) has been studied in primary care and community settings. Limitations regarding availability and quality of testing remain. A targeted spirometry screening program was implemented within a presurgical screening (PSS) clinic to detect undiagnosed airways disease and identify patients with COPD/asthma in need of treatment optimization. OBJECTIVE The present quality assurance study evaluated airflow obstruction detection rates and examined characteristics of patients identified through the targeted screening program. METHODS The targeted spirometry screening program was implemented within the PSS clinic of a tertiary care university hospital. Current or ex-smokers with respiratory symptoms and patients with a history of COPD or asthma underwent prebronchodilator spirometry. History of airways disease and smoking status were obtained during the PSS assessment and confirmed through chart reviews. RESULTS After exclusions, the study sample included 449 current or ex-smokers. Abnormal spirometry results were found in 184 (41%) patients: 73 (16%) had mild, 93 (21%) had moderate and 18 (4%) had severe or very severe airflow obstruction. One hundred eighteen (26%) new cases of airflow obstruction suggestive of COPD were detected. One-half of these new cases had moderate or severe airflow obstruction. Only 34% of patients with abnormal spirometry results had reported a previous diagnosis of COPD. More than one-half of patients with abnormal spirometry results were current smokers. CONCLUSIONS Undiagnosed airflow obstruction was detected in a significant number of smokers and ex-smokers through a targeted screening program within a PSS clinic. These patients can be referred for early intervention and secondary preventive strategies.
Type 2 Diabetes Prevention in the “Real World”

OBJECTIVE—“Real-world” implementation of lifestyle interventions is a challenge. The Good Ageing in Lahti Region (GOAL) Lifestyle Implementation Trial was designed for the primary health care setting, with lifestyle and risk reduction objectives derived from the major diabetes prevention efficacy trials. We report on the program's effectiveness as well as findings related to the program's reach, adoption, and implementation. RESEARCH DESIGN AND METHODS—A total of 352 middle-aged participants with elevated type 2 diabetes risk were recruited from the health care centers in Päijät-Häme Province in Finland. The intervention included six group counseling sessions, delivered by trained public health nurses. Measurement was conducted at baseline and 12 months. Clinical risk factors were measured by study nurses, and lifestyle outcomes were analyzed from self-reports. Lifestyle outcomes were compared with the outcomes achieved in relevant efficacy trials, and within-subject changes were tested for risk reduction. RESULTS—At baseline, mean BMI was >32 kg/m2, and 25% of the participants had impaired glucose tolerance. At 12 months, 20% of participants achieved at least four of five key lifestyle outcomes, with these results being comparable with the reference trials. However, physical activity and weight loss goals were achieved significantly less frequently (65 vs. 86% and 12 vs. 43%, respectively). Several clinical risk factors decreased, more so among men than women. CONCLUSIONS—This trial demonstrates that lifestyle counseling can be effective and is feasible in real-world settings for individuals with elevated risk of type 2 diabetes. To increase program impact, program exposure and treatment intensity need to be increased.
A systematic literature review on attractiveness and learnability factors in Web applications

User interface design is an essential element for consideration in Web application development. Usability (ISO/IEC 9126-1:2001) or operability (ISO/IEC 25010:2011) is one of non-functional requirements which must be taken into consideration when designing a user interface. Attractiveness and learnability are among the sub criteria under the operability factor. Analysis of these criteria may be used to evaluate user interface design. This study has adopted a systematic literature review method to study existing works concerning the two factors of operability. The outcome of the review provides insight into existing works on attractiveness and learnability factors in software applications generally and Web applications specifically.
Opportunities in Higher Education: An Application to France

This paper first sets out criteria to evaluate the inequality of opportunity in higher education. We propose a two-level classification by distinguishing the categorial and the counterfactual approaches. For each approach we consider dominance quasi-orderings and inequality measures. We then apply these criteria to the French higher education system by comparing the situations in 1992 and 2004. Our main findings suggest: (i) that there is inequality of opportunity in each of the two years; and (ii) that the inequality of opportunity tends to increase during the reporting period, especially the opportunity to graduate in the lowest and highest levels of education.
Loan Loss Provisioning, Income Smoothing, Signaling, Capital Management and Procyclicality: Does IFRS Matter? Empirical Evidence from Nigeria.

Prior research show that banks have various motivations for influencing loan loss provisions. This study examines these motivations and the behaviour of loan loss provision in relation to the business cycle. After controlling for the impact of Basel regulation on LLP, I find strong evidence for income smoothing, capital management and procyclical LLP behaviour during the voluntary, not mandatory, adoption of IFRS in Nigeria. I find evidence of signaling only after including interaction terms in the model. Additionally, I find that (i) banks increase loan loss provisioning after the implementation of Basel; (ii) banks have some incentive to signal via LLP in the post-IFRS period relative to the pre-IFRS period (iii) banks have joint motivations to manipulate LLP and may face trade-offs in the choice of managing regulatory capital or smoothing income in the post-IFRS period. Overall, I conclude that IFRS reinforces LLP motivations and procyclical patterns. The findings of this paper are relevant to current concerns of accounting standard setters and bank regulators on the current model of loan loss provisioning as well as the on-going debate on the mandatory implementation of IFRS in Nigeria. DOI: 10.5901/mjss.2015. v6n2p224
Functional Properties of the Separate Subunits of Human DNA Helicase II/Ku Autoantigen*

The Ku antigen consists of two subunits of 70 and 83 kDa and is endowed with both duplex DNA end-binding capacity and helicase activity (human DNA helicase II). HeLa Ku can be isolated fromin vitro cultured human cells uniquely as a heterodimer, and the subunits can be separated by electrophoresis only under denaturing conditions. To dissect the molecular functions of the two subunits of the heterodimer, we have cloned and expressed their cDNAs separately inEscherichia coli. The two activities of Ku (DNA binding and unwinding) were reconstituted by mixing and refolding both subunits in equimolar amounts (Tuteja, N., Tuteja, R., Ochem, A., Taneja, P., Huang, N-W., Simoncsits, A., Susic, S., Rahman, K., Marusic, L., Chen, J., Zang, J., Wang, S., Pongor, S., and Falaschi, A. (1994)EMBO J. 13, 4991–5001). Renaturation of the separate subunits can be achieved in the presence of a synthetic solubilizing and stabilizing agent, dimethyl ethylammonium propane sulfonate (NDSB 195). The helicase activity of the Ku protein resides uniquely in the 70-kDa subunit, whereas the DNA end-binding activity can be reconstituted only through renaturation of the two subunits in the heterodimeric form and is practically absent in the separate subunits. The 83-kDa subunit, when refolded in the absence of the 70-kDa subunit, forms homodimers unable to unwind DNA and bind duplex ends. The three separate species (heterodimer, 70-kDa subunit, and 83-kDa subunit homodimer) all have ssDNA-dependent ATPase activity.
College News

Join the American College of Medical Genetics (ACMG) executive director Michael S. Watson, PhD, FACMG; president-elect Bruce R. Korf MD, PhD, FACMG; and genetic counselor and Special Assistant to the Director, ACMG Judith Benkendorf, MS, CGC for a lively discussion of what is happening in genetic medicine and hear about exciting ACMG plans for 2009 and beyond. The field of genomics and its application to healthcare is developing at a dizzying pace – take an hour to hear about some of the most exciting developments on the front lines of clinical genetics from the ACMG and what they might mean for you.
Grammaticality is inferred from global similarity: A reply to Kinder (2010)

Jamieson and Mewhort (2009b) proposed an account of performance in the artificial-grammar judgement-of-grammaticality task based on Hintzman's (1986) model of retrieval, Minerva 2. In the account, each letter is represented by a unique vector of random elements, and each exemplar is represented by concatenating its constituent letter vectors. Although successful in simulating several experiments, Kinder (2010) showed that the model fails for three selected experiments. We track the model's failure to a constraint introduced by concatenating letter vectors to construct the exemplar representation. To fix the problem, we use a holographic representation. Holographic representation not only provides the flexibility missing with the concatenation scheme but also acknowledges variability in what subjects notice when they inspect training exemplars. Armed with holographic representations, we show that the model successfully captures the three problematic data sets. We argue for retrospective accounts, like the present one, that acknowledge subjects’ skill in drawing unexpected inferences based on memory of studied items against prospective accounts that require subjects to learn statistical regularities in the training set in anticipation of an undefined classification test.
Calcitonin‐gene related peptide is an evolutionarily conserved marker within the amniote thalamo‐telencephalic auditory pathway

The distribution of neurons and fibers containing calcitonin‐gene‐related peptide (CGRP) was mapped in the thalamo‐telencephalic auditory pathways of four amniote species, rats, pigeons (Columba livia), caiman (Caiman crocodilus), and turtles (Pseudemys scripta). In colchicine‐treated turtles and pigeons, numerous CGRP+ perikarya were observed in the auditory relay nucleus of the thalamus (n. reuniens of reptiles, and n. ovoidalis of birds). In pigeons, these neurons were most abundant in the outer circumference of the nucleus and were not observed without colchicine pretreatment. In the telencephalon of turtles, caiman, and pigeons, CGRP + fibers were observed within portions of the dorsal ventricular ridge previously shown to receive projections from the auditory thalamus, thus implying that the thalamic CGRP+ neurons observed here in fact project to these telencephalic areas. In colchicine treated rats, numerous CGRP+ perikarya were observed along the ventral margin of the medial geniculate nucleus extending into the posterior intralaminar and peripeduncular nuclei, as well as occasionally within the ventral subdivision of the medial geniculate nucleus. Injections of fluorogold into the auditory cortex combined with immunofluorescence labeling for CGRP revealed that CGRP+ cells in these areas do, in fact, project to the auditory cortices.
The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods.

BACKGROUND The epidemiology of tuberculosis in urban populations is changing. Combining conventional epidemiologic techniques with DNA fingerprinting of Mycobacterium tuberculosis can improve the understanding of how tuberculosis is transmitted. METHODS We used restriction-fragment-length polymorphism (RFLP) analysis to study M. tuberculosis isolates from all patients reported to the tuberculosis registry in San Francisco during 1991 and 1992. These results were interpreted along with clinical, demographic, and epidemiologic data. Patients infected with the same strains were identified according to their RFLP patterns, and patients with identical patterns were grouped in clusters. Risk factors for being in a cluster were analyzed. RESULTS Of 473 patients studied, 191 appeared to have active tuberculosis as a result of recent infection. Tracing of patients' contacts with the use of conventional methods identified links among only 10 percent of these patients. DNA fingerprinting, however, identified 44 clusters, 20 of which consisted of only 2 persons and the largest of which consisted of 30 persons. In patients under 60 years of age, Hispanic ethnicity (odds ratio, 3.3; P = 0.02), black race (odds ratio, 2.3; P = 0.02), birth in the United States (odds ratio, 5.8; P < 0.001), and a diagnosis of the acquired immunodeficiency syndrome (odds ratio, 1.8; P = 0.04) were independently associated with being in a cluster. Further study of patients in clusters confirmed that poorly compliant patients with infectious tuberculosis have a substantial adverse effect on the control of this disease. CONCLUSIONS Despite an efficient tuberculosis-control program, nearly a third of new cases of tuberculosis in San Francisco are the result of recent infection. Few of these instances of transmission are identified by conventional contact tracing.