Miracle-dz/UniFAR-FTUs huggingface dataset:
Differential Effects of DNA Supercoiling on Chlamydia Early Promoters Correlate with Expression Patterns in Midcycle

ABSTRACT Changes in DNA supercoiling levels during the chlamydial developmental cycle have been proposed as a global mechanism to upregulate midcycle genes, but the effects on early genes are not known. We examined the promoters for 10 Chlamydia trachomatis early genes and found that they could be separated into two subsets based on their responses to DNA supercoiling in vitro. Furthermore, the type of supercoiling response correlated with the in vivo expression pattern for each early gene. One subset of seven early genes had promoters that were transcribed in a supercoiling-insensitive manner over the physiologic range of supercoiling levels that have been measured in Chlamydia. In vivo transcripts for these genes were detected at similar levels during early-stage and midstage times. In contrast, a second subset, represented in our study by three early genes, had supercoiling-dependent promoters that were transcribed at higher levels from more-supercoiled templates, which is the response observed for midcycle genes. Genes in this subset were expressed at higher levels at midstage times than at early times in vivo. We propose that this second subset represents a novel class of chlamydial developmental genes with features of both early and midcycle genes. We hypothesize that expression of these supercoiling-dependent early genes is upregulated by increased chlamydial supercoiling levels in midcycle via their supercoiling-responsive promoters in a manner similar to that for midcycle genes. Thus, we propose that DNA supercoiling is utilized in Chlamydia as a general mechanism to regulate genes in the midstage of the developmental cycle and not just midcycle genes.
Software development of optimal substation ground grid design based on genetic algorithm and pattern search

Substation ground system serves to safety of personnel and as major equipment during earth faults, which deserves considerable attentions. Basic substation safety assessment quantities include ground grid resistance, mesh touch potential and step potential, moreover, optimal design of substation ground system should consider both safeness and cost. To fill the lack of such suitable and economical optimal design software in North American industry, a software package coded in MATLAB is developed and its core algorithm and main features are introduced in this paper. A novel hybrid GA-PS optimization or two-steps optimization method is developed, in which Genetic Algorithm (GA) is used firstly to search an approximate start point range for the further optimal searching, followed by Pattern Search (PS) to find the final optimal result. This software can give analysis of ground grid safety performance and is able to recommend optimal grid design for given safety constraints. In order to make sure the accuracy of this software, the results of grid safety assessment obtained here are also compared with the results calculated by using worldwide used software WinIGS.
An Overview of Object-Oriented Simulation

With an immense increase in research, development, and application of object- oriented simulation (OOS) over the past decade, there is evidence that this technology is becoming the choice for modeling large, complex, and/or distributed systems. This paper discusses the features of OOS that dispose it to modeling these types of systems and compares the OOS approach with traditional simulation approaches. A review of OOS languages and environments is followed by a discussion of current issues pertaining to object- oriented simulation.
Developing an Industrial IoT Platform - Trade-off between Horizontal and Vertical Approaches

Demands for a digitalized, connected, and smart production provide a fertile ground for industrial Internet-of-Things (IIoT) platforms to arise within the manufacturing industry (e.g., Siemens MindSphere, AXOOM Smart Enterprise, FORCAM FORCE). Nevertheless, many companies struggle to successfully kick-off platform ecosystems. Information Systems (IS) literature is of limited help, because insights on managing platform ecosystems are mostly derived from successful examples in the business-to-consumer (B2C) context. To better understand the challenging situation of companies in the emerging IIoT environment, we conducted an in-depth case study at a prospective platform provider. Insights gained through interviews and engagement in the field uncovered a tension between a horizontal platform strategy and vertical integrated solutions as a central challenge for companies aiming to launch an IIoT-platform in the market. By conceptualizing this trade-off, its causes along with related benefits and challenges, we add to existing literature on platform governance and launch strategies.
MOLECULAR BIOLOGY New Dinucleotide and Trinucleotide Microsatellite Marker Resources for Cotton Genome Research

A collaborative multi-institutional program was initiated to streamline the process of microsatellite capture and characterization, development of microsatellites into informative molecular markers, and dissemination of marker information to the cotton research community. A simple and efficient biotin capture method was optimized and used to capture more than 10,000 fragments. Out of 588 fragments sequenced, nearly all contained a microsatellite repeat structure. Several repeat types were represented, including AGA, GA, CA, and ACA. Primers were designed to amplify 307 unique microsatellite loci (305 nuclear and two chloroplast-encoded). One hundred fifty-two microsatellite loci were amplified from G. hirsutum L. cv. TM-1 and Tamcot SP37, and G. barbadense L. cv. Pima 3-79 and Pima S-7. In this comparison, 74 of the primers (approximately 49% of the subset) showed detectable polymorphism. In a comparison of upland G. hirsutum cultivars, approximately 26% of the primers exhibited intraspecific polymorphism. Polymorphism was widely distributed among the various repeat types and structures (e.g., imperfect and compound repeats). Redundancy with two other previously derived microsatellite marker sets (BNL, CM) was low, implying that the total pool of microsatellites present in the cotton genome is large enough to satisfy the requirements of extensive genome mapping and marker-assisted selection projects.
The Sense of Beauty

This paper proposes an integrative psychoanalytic model of the sense of beauty. The following definition is used: beauty is an aspect of the experience of idealisation in which an object(s), sound(s) or concept(s) is believed to possess qualities of formal perfection. The psychoanalytic literature regarding beauty is explored in depth and fundamental similarities are stressed. The author goes on to discuss the following topics: (1) beauty as sublimation: beauty reconciles the polarisation of self and world; (2) idealisation and beauty: the love of beauty is an indication of the importance of idealisation during development; (3) beauty as an interactive process: the sense of beauty is interactive and intersubjective; (4) the aesthetic and non‐aesthetic emotions: specific aesthetic emotions are experienced in response to the formal design of the beautiful object; (5) surrendering to beauty: beauty provides us with an occasion for transcendence and self‐renewal; (6) beauty's restorative function: the preservation or restoration of the relationship to the good object is of utmost importance; (7) the self‐integrative function of beauty: the sense of beauty can also reconcile and integrate self‐states of fragmentation and depletion; (8) beauty as a defence: in psychopathology, beauty can function defensively for the expression of unconscious impulses and fantasies, or as protection against self‐crisis; (9) beauty and mortality: the sense of beauty can alleviate anxiety regarding death and feelings of vulnerability. In closing the paper, the author offers a new understanding of Freud'semphasis on love of beauty as a defining trait of civilisation. For a people not to value beauty would mean that they cannot hope and cannot assert life over the inevitable and ubiquitous forces of entropy and death.
GENETIC EFFECTS OF RADIOTHERAPY FOR CHILDHOOD CANCER

Abstract— Radiation-induced heritable diseases have not been demonstrated in humans and estimates of genetic risks for protection purposes are based on mouse experiments. The most comprehensive epidemiologic study is of the Japanese atomic bomb survivors and their children, which found little evidence for inherited defects attributable to parental radiation. Studies of workers exposed to occupational radiation or of populations exposed to environmental radiation appear too small and exposures too low to convincingly detect inherited genetic damage. In contrast, survivors of childhood cancer form the largest group of people exposed to high doses of ionizing radiation before reproduction and offer unique advantages for studying trans-generation effects. A wide range of gonadal doses are possible, several comparison groups are readily available (including siblings), and there is a strong willingness among cancer survivors to participate in health studies. Cancer patients also have detailed medical records that facilitate both the accurate estimation of gonadal doses and the assessment of potentially confounding factors, such as intercurrent illness, personal and family medical histories, lifestyle characteristics such as tobacco use, and circumstances at delivery. An international study is nearing completion of over 25,000 survivors of childhood cancer in the United States and Denmark who gave birth to or fathered over 6,000 children. Doses to gonads are being reconstructed from radiotherapy records with 46% over 100 mSv and 16% over 1,000 mSv. Adverse pregnancy outcomes being evaluated include major congenital malformations, cytogenetic abnormalities, stillbirths, miscarriages, neonatal deaths, total deaths, leukemia and childhood cancers, altered sex ratio, and birth weight. The main analyses are based on dose-response evaluations. Blood studies of trios (cancer survivor, spouse or partner and offspring) have been initiated to evaluate mechanistic evidence for the transmission of any radiation-induced genetic damage such as minisatellite mutations. Markers of cancer susceptibility such as chromosomal radiosensitivity and genotype profile will also be examined. In the United States series to date, 4,214 children were born to cancer survivors among whom 157 (3.7%) genetic diseases were reported in contrast to 95 (4.1%) reported conditions among 2,339 children born to sibling controls. In the Denmark series the comparable figures were 82 (6.1%) birth defects among 1,345 children of cancer survivors and 211 (5.0%) among 4,225 children of sibling controls. Coupled with prior studies, these preliminary findings, if sustained by ongoing dose-response analyses, provide reassurance that cancer treatments including radiotherapy do not carry much if any risk for inherited genetic disease in offspring conceived after exposure.
[Placental histopathology in premature rupture of membranes. Its relationship with microbiological findings, maternal, and neonatal outcome].

BACKGROUND There is a close relationship between premature membrane rupture, bacterial infections and premature labor. AIM To study placental histological changes in patients with preterm membrane rupture. To establish a relationship between pathological findings, amniotic fluid and lower genital tract microbiological studies, maternal and neonatal outcome. PATIENTS AND METHODS Patients with premature membrane rupture of membranes between 24 and 34 weeks of gestation participated in this study. On admission, patients had no evidence of clinical chorioamnionitis, labor or fetal distress. Microbiological studies of the amniotic fluid and cervicovaginal secretions were performed and the placenta was sent for pathological study. RESULTS Seventy one placentas were available for the study. The main pathological findings were acute chorioamnionitis in 58%, trophoblastic proliferation in 38%, funisitis in 37%, villitis in 16%, fetal vascular lesions in 14% and no findings in 17%. Microbial invasion of amniotic cavity was present in 89% of acute chorioamnionitis. Sixty one percent of trophoblastic proliferation and all fetal vascular lesions were associated with negative amniotic and cervical cultures. Newborns with acute funisitis had a higher frequency of neonatal death (29%), severe asphyxia (42%) and neonatal infections (29%). CONCLUSIONS Acute chorioamnionitis is the most frequent finding in patients with preterm membrane rupture and microbial invasion of amniotic cavity. In the absence of intra amniotic infection, proliferation of the trophoblast and the presence of fetal vascular lesions predominate. Acute funisitis is strongly associated with adverse fetal outcome.
Institutional logics as inhibitors or levers? The case of mobile payments in Finland

Purpose: This paper aims to explore what kind of institutional barriers companies must overcome in order to innovate a new mobile payment service. Design/methodology/approach: A qualitative case study of mobile payment development in Finnish financial sector is applied to explore institutional factors affecting innovation in payment services. Findings: Institutional factors (cultural-cognitive, normative, and regulative) affect innovation of mobile payment services in highly institutionalized setting. In addition to defining those barriers, in this study we find that startups can use institutional barriers of traditional incumbents as levers for their own innovations. Research limitations/implications: The study deepens our current understanding of the culturalcognitive, normative and regulative factors affecting of new practices and service innovations in the highly institutionalized setting. Practical implications: Results of this research will help startups to find their levers as well as incumbents to identify the barriers for change. The change also means a transition from goodsoriented business model to serviceand customer-dominant thinking. Originality/value: This research contributes to the discussion about FinTech as a phenomenon and broadens the general understanding of related change processes.
Aldosterone and cortisol predict medium‐term left ventricular remodelling following myocardial infarction

Mineralocorticoid receptor (MR) antagonists improve cardiovascular outcomes in patients with heart failure complicating acute myocardial infarction (AMI) and in chronic heart failure. It is unclear whether these beneficial effects are due solely to aldosterone blockade, as MR has a similar affinity for cortisol. We examined the relationships between plasma and urinary steroid hormones and left ventricular (LV) remodelling in patients with LV dysfunction following AMI.
PENGARUH TIPE INDUSTRI, PROFITABILITAS, MEDIA EXPOSURE, UKURAN PERUSAHAAN, DAN LEVERAGE TERHADAP CARBON EMISSION DISCLOSURE

This study aims to obtain empirical evidence on the analysis of factors affecting the voluntary disclosure of carbon emissions in companies in Indonesia. Factors used include industry type, profitability, firm size and leverage. Measurement of carbon emission disclosure levels using content analysis. There are 18 items to detect disclosure rates of carbon emissions. The population of this research is all financial data of non service industry companies listed on BEI period 2014-2016. Sampling method used in this research is purposive sampling method. The sample used in this research is non service industry which publishes annual and financial report during observation period and discloses carbon emission. The total sample used in this research is 27 companies. The data used is secondary data from IDX. The analysis technique used is multiple linear regression analysis. The results of this study indicate that industry type and company size have an effect on carbon emission disclosure, while profitability proxy with return on asset and return on equity and leverage have no effect on carbon emission disclosure.
Using SOM to Mine Product Features from Free-Text Customer Reviews

This study examines how the Self-Organizing Map (SOM) technique can be used to identify product features from free-text customer reviews. A novel SOM display named “Attribute Accumulative Matrix” is presented. To verify the validity of the proposed approach, 22157 restaurant reviews are collected and product features of catering industry are identified. The experiment results show that this approach can effectively identify the product features.
Getting maintenance of certification to work: a grounded theory study of physicians' perceptions.

IMPORTANCE Despite general support for the goals of maintenance of certification (MOC), concerns have been raised about its effectiveness, relevance, and value. OBJECTIVE To identify barriers and enabling features associated with MOC and how MOC can be changed to better accomplish its intended purposes. DESIGN, SETTING, AND PARTICIPANTS Grounded theory focus group study of 50 board-certified primary care and subspecialist internal medicine and family medicine physicians in an academic medical center and outlying community sites. EXPOSURES Eleven focus groups. MAIN OUTCOMES AND MEASURES Constant comparative method to analyze transcripts and identify themes related to MOC perceptions and purposes and to construct a model to guide improvement. RESULTS Participants identified misalignments between the espoused purposes of MOC (eg, to promote high-quality care, commitment to the profession, lifelong learning, and the science of quality improvement) and MOC as currently implemented. At present, MOC is perceived by physicians as an inefficient and logistically difficult activity for learning or assessment, often irrelevant to practice, and of little benefit to physicians, patients, or society. To resolve these misalignments, we propose a model that invites increased support from organizations, effectiveness and relevance of learning activities, value to physicians, integration with clinical practice, and coherence across MOC tasks. CONCLUSIONS AND RELEVANCE Physicians view MOC as an unnecessarily complex process that is misaligned with its purposes. Acknowledging and correcting these misalignments will help MOC meet physicians' needs and improve patient care.
Psychosocial Interventions for Depression and Anxiety in Parkinson's Disease

Background: Depression has been estimated to affect 1 in 3 individuals with Parkinson's disease (PD) and can lead to worse health outcomes and decreased quality of life. Anxiety further complicates PD outcomes. Pharmacologic treatments of depression and anxiety can have negative side effects in patients with PD, including exacerbation of PD symptoms. There is a critical need for alternative treatment approaches that address depression and anxiety among patients with PD. Psychosocial or behavioral approaches are known to be effective for depression generally, but only a handful of studies have examined the role of psychosocial treatments of patients with depression in PD. Objective: The aim of this article was to review published psychosocial treatment studies of depression and anxiety in patients with PD. Methods: The PubMed database was searched for articles published in English before April 2011 using the terms Parkinson’s disease, depression, anxiety, psychotherapy, cognitive therapy, behavioral treatment, behavioral therapy, nonpharmacologic treatment, psychoeducation, education, psychosocial treatment, and stress management. Articles included were prospective clinical trials utilizing specific depression or anxiety assessments as primary or secondary outcomes in patients with PD. Results: Nine reports derived from 8 separate studies fit the inclusion criteria and were included in this review. All studies were published between 1997 and 2011, and all but 3 had small sample sizes (<40 patients). Interventions included cognitive behavioral therapy (CBT), psychodrama, education, and behavior therapy as well as multidisciplinary rehabilitation. Cognitive behavioral therapy was the most studied and was shown to decrease depressive and anxiety symptoms in patients with PD. Other individual and group therapies may improve depression and anxiety in patients with PD, but the general paucity of studies and study methods limit the interpretation of these results. Conclusions: There have been few studies on psychosocial treatments that specifically assess change in depression and anxiety among patients with PD. While results for CBT and other modes of therapy are promising for acute management of depression and anxiety, longer term effects after treatment have been variable. There is a need for additional studies on psychosocial interventions in people with PD.
Mitochondrial DNA mapping of social-biological interactions in Brazilian Amazonian African-descendant populations

The formation of the Brazilian Amazonian population has historically involved three main ethnic groups, Amerindian, African and European. This has resulted in genetic investigations having been carried out using classical polymorphisms and molecular markers. To better understand the genetic variability and the micro-evolutionary processes acting in human groups in the Brazilian Amazon region we used mitochondrial DNA to investigate 159 maternally unrelated individuals from five Amazonian African-descendant communities. The mitochondrial lineage distribution indicated a contribution of 50.2% from Africans (L0, L1, L2, and L3), 46.6% from Amerindians (haplogroups A, B, C and D) and a small European contribution of 1.3%. These results indicated high genetic diversity in the Amerindian and African lineage groups, suggesting that the Brazilian Amazonian African-descendant populations reflect a possible population amalgamation of Amerindian women from different Amazonian indigenous tribes and African women from different geographic regions of Africa who had been brought to Brazil as slaves. The present study partially mapped the historical biological and social interactions that had occurred during the formation and expansion of Amazonian African-descendant communities.
The feasibility and farmer perception of true shallot seed technology in Sigi District, Central Sulawesi, Indonesia

Abstract. Rahayu HSP, Muchtar, Saidah. 2019. The feasibility and farmer perception of true shallot seed technology in Sigi District, Central Sulawesi, Indonesia. Asian J Agric 3: 16-21. Shallot is one of horticultural commodities that plays a significant role in both national and regional economy. A fluctuating supply of shallot influences the inflation level. Shallot production is currently still facing many problems, including high production cost. The high shallot production cost mostly goes to expenses for labor and seed while Indonesian shallot is mainly produced from the bulbs seed. This high-cost production causes a lower shallot competitiveness. Therefore, introduction of True Shallot Seed (TSS) technology, which lowers the cost for shallot seed, could be an ideal option to improve the shallot competitiveness in Indonesia. However, the shallot farming feasibility and the farmer’s perception of this technology are two critical aspects that need to be considered in the adoption of this new technology. This research aimed to study the potency of true shallot seed development in Central Sulawesi based on the TSS’s farming feasibility and farmer perception on TSS. The research was conducted in Sigi District, Central Sulawesi. The results showed that the farming of shallot using TSS was feasible, and within 14.9 t.ha-1 productivity, the Revenue-Cost Ratio was 3.15 while the Benefit-Cost Ratio was 2.15. The perception was examined based on three aspects namely technical, economic, and social aspects. The results showed that farmers were interested in planting true seed of shallot based on its high productivity, lower production cost, and market acceptance of the product; while in the social aspect, the extension and farmer group’s support still need to be improved for development of TSS.