Miracle-dz/UniFAR-FTUs huggingface dataset:
A Quality Improvement Cycle: Hemolyzed Specimens in the Emergency Department

OBJECTIVE: To determine the cause of and possible solution for an excessive number of hemolyzed specimens received from the emergency department (ED) of a large medical center. DESIGN: The clinical laboratory staff collected data on hemolyzed specimens for all departments of the medical center. The clinical laboratory management team and ED management team intervened with training and surveillance of the ED staff to heighten the awareness of the problem. SETTING: The clinical chemistry laboratory of a large medical center. MAIN OUTCOME MEASURE: The number of specimens submitted by inpatient departments and the ED was measured in relationship to the number of hemolyzed specimens received from the departments. The clinical laboratory measured specimen processing times and turnaround times to determine their role in possibly contributing to the large number of hemolyzed specimens. Direct observation by a certified phlebotomist documented anecdotal evidence of the ED staff's phlebotomy practices. ED and clinical laboratory practitioners communicated realistic impressions of the medical centers problem with hemolyzed specimens. RESULTS: The laboratory processing times were not responsible for the hemolyzed specimens. The collection equipment was not responsible for the hemolyzed specimens. The ED had an excessive number of hemolyzed specimens when compared to the rest of the medical center. The collection techniques in the ED appeared to be the origin of the problem. CONCLUSION: The intervention of the laboratory manager with the ED chief and nurse manager abated some of the professional arrogance between the departments. The dialogue educated the staffs about specific data that pointed to a possible origin of the problem. The ED chief placed his department on surveillance against problematic draws. Communication was improved between the two departments. However, only a moderate improvement in the number of hemolyzed specimens was noted. More training of medical center departments in phlebotomy and periodic proficiency evaluation of the all staff was indicated as a possible long-term solution. ABBREVIATIONS: CLT = clinical laboratory technician; CLS = clinical laboratory scientist; ED = emergency department; HIS = hospital information system; LIS = laboratory information system; RBC = red blood cells; SOP = standard operating procedure; TAT = turnaround time.
Molecular dynamics simulations on the Tre 1 G protein-coupled receptor : Exploring the role of the arginine of the NRY motif in Tre 1 structure

Background: The arginine of the D/E/NRY motif in Rhodopsin family G protein-coupled receptors (GPCRs) is conserved in 96% of these proteins. In some GPCRs, this arginine in transmembrane 3 can form a salt bridge with an aspartic acid or glutamic acid in transmembrane 6. The Drosophila melanogaster GPCR Trapped in endoderm-1 (Tre1) is required for normal primordial germ cell migration. In a mutant form of the protein, Tre1, eight amino acids RYILIACH are missing, resulting in a severe disruption of primordial germ cell development. The impact of the loss of these amino acids on Tre1 structure is unknown. Since the missing amino acids in Tre1 include the arginine that is part of the D/E/NRY motif in Tre1, molecular dynamics simulations were performed to explore the hypothesis that these amino acids are involved in salt bridge formation and help maintain Tre1 structure. Results: Structural predictions of wild type Tre1 (Tre1) and Tre1 were subjected to over 250 ns of molecular dynamics simulations. The ability of the model systems to form a salt bridge between the arginine of the D/E/NRY motif and an aspartic acid residue in transmembrane 6 was analyzed. The results indicate that a stable salt bridge can form in the Tre1 systems and a weak salt bridge or no salt bridge, using an alternative arginine, is likely in the Tre1 systems. Conclusions: The weak salt bridge or lack of a salt bridge in the Tre1 systems could be one possible explanation for the disrupted function of Tre1 in primordial germ cell migration. These results provide a framework for studying the importance of the arginine of the D/E/NRY motif in the structure and function of other GPCRs that are involved in cell migration, such as CXCR4 in the mouse, zebrafish, and chicken.
Contraceptive Methods, Seizures, and Antiepileptic Drugs: More Questions than Answers

OBJECTIVE: To report the contraceptive practices of women with epilepsy (WWE) in the community, predictors of highly effective contraception use, and reasons WWE provide for the selection of a particular method. METHODS: These cross-sectional data come from the Epilepsy Birth Control Registry (EBCR) web-based survey regarding the contraceptive practices of 1,144 WWE in the community, ages 18–47 years. We report demographic, epilepsy, and antiepileptic drug (AED) characteristics as well as contraceptive use. We determined the frequency of use of highly effective contraception use, that is, methods with failure rate <10%/year, and conducted binary logistic regression analysis to determine predictors of highly effective contraception use. We report frequencies of WWE who consult various health care providers regarding the selection of a method and the reasons cited for selection. RESULTS: Of the 796 WWE at risk of unintended pregnancy, 69.7% use what is generally considered to be highly effective contraception (hormonal, intrauterine device [IUD], tubal, vasectomy). Efficacy in WWE, especially for the 46.6% who use hormonal contraception, remains to be proven. Significant predictors of highly effective contraception use are insurance (insured 71.6% vs. noninsured 56.0%), race/ethnicity (Caucasian 71.3% vs. minority 51.0%), and age (38–47, 77.5%; 28–37, 71.8%; 18–27, 67.0%). Of the 87.2% who have a neurologist, only 25.4% consult them regarding selection of a method, although AED interaction is cited as the top reason for selection. SIGNIFICANCE: The EBCR web-based survey is the first large-scale study of the contraceptive practices of WWE in the community. The findings suggest a need for the development of evidence-based guidelines that address the efficacy and safety of contraceptive methods in this special population, and for greater discourse between neurologists and WWE regarding contraception.
Pattern of admission to hospitals during muslim pilgrimage (Hajj).

OBJECTIVE The pattern of medical conditions necessitating admission differs according to the weather condition in that particular year. Previous studies had been conducted during the hot weather, none over the last 10 years, were carried out during, the milder weather we are experiencing. The aim of this study is to establish the pattern of admission during this mild weather and to elucidate the possible risk factors. METHODS A prospective study was performed in 4 hospitals in 2 different locations in Al-Mashaer, Kingdom of Saudi Arabia. Data were collected during one working shift in 2 hospitals in Arafat on the 9th of Dhul Hijjah 1422, corresponding to 21st of February 2002, and another working shift in 2 hospitals in Mina on the 10th of Dhul Hijjah 1422, corresponding to 22nd of February 2002. RESULTS A cohort of 76 patients from Arafat hospitals and 84 patients from Mina hospitals were included (total 160 patients). Males constituted 62% and females 38% with the median age of 60 +/- 15 years. The respiratory system was the most commonly affected (57%), followed by cardiovascular system (19.4%), and gastrointestinal tract (GIT) in 6.3% of cases. There were only 3 cases of heat-related admissions with only one confirmed case of heat stroke. Similarly, only one case of meningitis was confirmed in this cohort. Pneumonia was encountered in 63 cases (39.4%) and exacerbation of asthma and chronic obstructive pulmonary diseases (COPD) in 23 cases (14.4%). Pre-existing co-morbid medical conditions had included bronchial asthma and COPD (22.5%), hypertension (17.5%), and Diabetes mellitus (15%). Short-term follow up (24-48 hours) identified 2 deaths (1.3%), 94 patients (59%) were transferred to other secondary or tertiary care facilities and 64 (40%) were discharged home. CONCLUSION Hospital admission during Hajj is related to old age and occurs in patients with associated co-morbid conditions. During this mild weather lower respiratory tract infections and exacerbation of bronchial asthma and COPD are the most commonly encountered diseases during Hajj.
What to Tell About Me? Self-Presentation in Online Communities

This paper investigates how the type of community and personal goals affect self-presentation. In 3 online studies, we simulated the registration process in online communities, presenting either a common-bond or a common-identity community. Study 1 confirmed that members of the common-bond community presented themselves in an individualizing manner, while members of the common-identity community focused on characteristics shared among members of the community. Study 2 investigated underlying processes, showing that the goal to get in contact with other members was less salient in the common-identity than in the common-bond community. Study 3 demonstrated that community members actively manage their self-presentation in accordance with personal goals. Based on these findings, we discuss implications for research and profile design. © 2012 Wiley Periodicals, Inc.
Phytoplankton traits from long-term oceanographic time-series

Trait values are usually extracted from laboratory studies of single phytoplankton species, which presents challenges for understanding the immense diversity of phytoplankton species and the wide range of dynamic ocean environments. Here we use a Bayesian approach and a trait-based model to extract trait values for four functional types and ten diatom species from field data collected at Station L4 in the Western Channel Observatory. We find differences in maximum net growth rate, temperature optimum and sensitivity, half-saturation constants for light and nitrogen, and density-dependent loss terms across the functional types. We find evidence of very high linear loss rates, suggesting that grazing may be even more important than commonly assumed and differences in density-dependent loss rates across functional types, indicating the presence of strong niche differentiation among functional types. Low half-saturation constants for nitrogen at the functional type level may indicate widespread mixotrophy. At the species level, we find a wide range of density-dependent effects, which may be a signal of diversity in grazing susceptibility or biotic interactions. This approach may be a way to obtain more realistic and better-constrained trait-values for functional types to be used in ecosystem modeling.
High risk of a large measles outbreak despite 30 years of measles vaccination in The Netherlands

SUMMARY Our aim was to assess progress towards measles elimination from The Netherlands by studying humoral measles immunity in the Dutch population. A population-based seroepidemiological study was conducted in 2006–2007 (N = 7900). Serum samples were analysed by a bead-based multiplex immunoassay. IgG levels ⩾0·2 IU/ml were considered protective. The overall seroprevalence in the Dutch population was 96%. However, 51% of socio-geographically clustered orthodox Protestant individuals aged <10 years were susceptible. Infants might be susceptible to measles between ages 4 months and 14 months, the age at which maternal antibodies have disappeared and the first measles, mumps, rubella (MMR) vaccination is administered, respectively. Waning of antibody concentrations was slower after the second MMR vaccination than after the first. The Netherlands is at an imminent risk of a measles outbreak in the orthodox Protestant minority. To prevent subsequent transmission to the general population, efforts to protect susceptible age groups are needed.
Comparison of Vitek GNI and GNI+ Cards for Identification of Gram-Negative Bacteria

ABSTRACT The GNI+ card has been developed by bioMerieux Vitek as an improvement over the GNI card for the identification of certain species of aerobic and facultative anaerobic bacteria. In this study, we tested 304 organisms from 30 different species on both the GNI and GNI+ cards. The GNI card correctly identified 285 (93.8%) of the isolates tested, and the GNI+ card correctly identified 287 (94.4%) of the isolates tested. The average time to reporting was 4.1 h for the GNI+ card compared to 5.7 h for the GNI card (P < 0.001). Overall, the GNI and GNI+ cards were comparable in identifying the organisms in this study while the GNI+ card gave substantially faster final test results.
Regional and global volumes of glaciers derived from statistical upscaling of glacier inventory data

[1] Very few global-scale ice volume estimates are available for mountain glaciers and ice caps, although such estimates are crucial for any attempts to project their contribution to sea level rise in the future. We present a statistical method for deriving regional and global ice volumes from regional glacier area distributions and volume area scaling using glacier area data from ∼123,000 glaciers from a recently extended World Glacier Inventory. We compute glacier volumes and their sea level equivalent (SLE) for 19 glacierized regions containing all mountain glaciers and ice caps on Earth. On the basis of total glacierized area of 741 × 103 ± 68 × 103 km2, we estimate a total ice volume of 241 × 103 ± 29 × 103 km3, corresponding to 0.60 ± 0.07 m SLE, of which 32% is due to glaciers in Greenland and Antarctica apart from the ice sheets. However, our estimate is sensitive to assumptions on volume area scaling coefficients and glacier area distributions in the regions that are poorly inventoried, i.e., Antarctica, North America, Greenland, and Patagonia. This emphasizes the need for more volume observations, especially of large glaciers and a more complete World Glacier Inventory in order to reduce uncertainties and to arrive at firmer volume estimates for all mountain glaciers and ice caps.
Use of Psychotropic Medication Groups in People with Severe Mental Illness and Stressful Childhood Experiences

Stressful childhood experiences (SCE) are associated with a variety of health and social problems. In people with severe mental illness (SMI) traumatic childhood experiences have been linked to more severe and treatment refractory forms of psychiatric symptoms, including psychotic symptoms. This study evaluates the use of psychotropic medication groups in a population of people with SMI and SCE, testing the association between SCE and prescription medication in an SMI population. A sample of 183 participants with SMI was divided into 2 exposure groups: high SCE (4 to 7 categories of SCE) and low SCE (0 to 3 categories of SCE). Both groups were compared in regard to prescribed dosing of psychotropic medications (antipsychotics, mood stabilizers, antidepressants, and anxiolytics/hypnotics). Participants who endorsed high SCE received higher doses of antipsychotic medications and mood stabilizers than those with low exposure. The results demonstrate that people with higher SCE categories received a higher dosing of psychotropic medication, specifically antipsychotic medication and mood stabilizers.
Mental health implications of human attachment to companion animals.

OBJECTIVES Because of the contradictory nature of findings and methodological weaknesses identified within current human-companion animal bond research, there is a need to further explore the connection between human-animal bonds and mental health. DESIGN The purpose of this survey questionnaire study was to explore the relationship of attachment to companion animal and human psychological distress after controlling for demographic variables, and to investigate whether the relationship between social supports and psychological distress would be moderated by attachment to a companion animal. RESULTS Results highlight the psychological vulnerability of individuals reporting a strong bond with their companion animal. CONCLUSIONS These findings are discussed in terms of their implications for mental health services designed to anticipate and address client-related companion animal needs.
Isolation and characterization of new facultatively alkalophilic strains of Bacillus species

Four facultatively alkalophilic isolates were purified from enrichment cultures initiated with lime-treated garden soil. Four isolates, OF1, OF3, OF4, and OF6, were obligately aerobic, spore-forming, gram-positive, motile rods which were capable of growth at both pH 7.5 and pH 10.5. Strains OF1 and OF6 grew best at the lower pH value; and whereas growth of these strains at pH 10.5 was completely dependent on added Na+, growth at pH 7.5 was only partially dependent on added Na+. Strains OF3 and OF4 grew better at pH 10.5 than at pH 7.5, with strain OF3 growing modestly over its entire pH range, while OF4 grew well. Growth of OF3 and OF4 was completely dependent on added Na+ at both pH 7.5 and pH 10.5. DNA-DNA hybridization studies indicated that OF1 and OF6 are closely related strains but are not related to the other isolates, Bacillus subtilis, or two previously studied obligately alkalophilic bacilli. OF3 was unrelated to any of the other organisms examined in the study, whereas OF4 showed complete homology with obligately alkalophilic Bacillus firmus RAB. All four isolates maintained a cytoplasmic pH that was considerably lower than the external pH when the latter was 10.5. Although substantial transmembrane electrical potentials were observed, the total electrochemical proton gradient (delta mu H+) was low at pH 10.5 in all the strains. By contrast, delta mu H+ was substantial at pH 7.5 and at that pH was composed entirely of an electrical potential. These results are in contrast to previous findings that obligately alkalophilic bacilli generate only small electrical potentials at near neutral pH. All the isolates exhibited substantial rates of respiration as measured by oxygen consumption. Neither respiration nor NADH oxidation by everted membrane vesicles was significantly stimulated by Na+. Analyses of reduced versus oxidized difference spectra of membranes from OF4 showed that the total membrane cytochrome content was considerably higher in cells grown at pH 10.5 than at pH 7.5, with the levels of c- and a-type cytochromes exhibiting the largest pH-dependent differences. Initial examination of membrane protein profiles on gel electrophoresis also indicated a number of changes in pattern in each isolate, depending on the growth pH.
Attributions of Athletes on Collegiate Sports Teams

This study investigated the patterns of attributions for success made by intercollegiate athletes in three categories: more versus less successful teams, men versus women athletes, and individual versus group sport participants. 74 men and 83 women on 17 varsity teams across a number of sports took the Sports Attribution Style Scale, measuring internal, stable, controllable, intentional, and global attributions. It was hypothesized that (a) the athletes on more successful teams would have higher internal, stable, controllable, and intentional attributions, (b) that athletes in individual sports would have lower internal, stable, and controllable attributions, and (c) that women would score higher on all five attributions. Profile analyses of the attributions led to the confirmation of the first hypothesis. For the second hypothesis, the pattern of sport attributions did not differ for individual and team athletes. The third hypothesis was not confirmed. Women athletes scored higher on the attributional dimensions (except globality).
New HRM Practices in the Public and Private Sector Industrial Enterprises of Bangladesh: A Comparative Assessment

This study explored adoption of new HRM practices in the public and private sector organizations of Bangladesh. We collected data from sixty industrial enterprises located in the main industrial city of country, i.e. Chittagong. The study revealed significant difference on adoption of new HRM practices between public and private sector industrial enterprises of Bangladesh. Employees in the private sector organizations appeared to be more satisfied than public sector organizations with organizational HRM practices.
In : Mechanisms of Disease in Small Animal Surgery

Some of the more challenging but also most rewarding cases in the busy general practice are the bitches presenting with suspected, or confirmed dystocia, or where the owners ask for an elective Cesarean section. Often both the welfare of the mother and the chances for survival of the pups are dependent on the accurate assessments and informed decisions made by the veterinarians and the staff at the clinic. To diagnose dystocia, a thorough appreciation of the various stages of the normal parturition – eutocia in the bitch is vital.
A fragment of secreted Hsp 90 a carries properties that enable it to accelerate effectively both acute and diabetic wound healing in mice

Wounds that fail to heal in a timely manner, for example, diabetic foot ulcers, pose a health, economic, and social problem worldwide. For decades, conventional wisdom has pointed to growth factors as the main driving force of wound healing; thus, growth factors have become the center of therapeutic developments. To date, becaplermin (recombinant human PDGFBB) is the only US FDA-approved growth factor therapy, and it shows modest efficacy, is costly, and has the potential to cause cancer in patients. Other molecules that drive wound healing have therefore been sought. In this context, it has been noticed that wounds do not heal without the participation of secreted Hsp90a. Here, we report that a 115-aa fragment of secreted Hsp90a (F-5) acts as an unconventional wound healing agent in mice. Topical application of F-5 peptide promoted acute and diabetic wound closure in mice far more effectively than did PDGF-BB. The stronger effect of F-5 was due to 3 properties not held by conventional growth factors: its ability to recruit both epidermal and dermal cells; the fact that its ability to promote dermal cell migration was not inhibited by TGF-b; and its ability to [. ] Research Article Dermatology
Microfocus Computed Tomography Analysis of Early Changes in Bone Microstructure in Rats with Chronic Renal Failure

Background/Aims: Little is known about the microstructural changes in bone tissue in the early phase of chronic renal failure (CRF). In this study, we analyzed the bone tissue in a rat model of early CRF using a high-resolution X-ray tomographic system, i.e. microfocus computed tomography (MXCT), which is capable of detecting microscopic structural changes in bone tissue. Methods: CRF rats were created by 7/8 nephrectomy by ligation of the renal artery branches, and were sacrificed 24 weeks after surgery. MXCT and bone mineral densitometry by single-energy X-ray absorptiometry (SXA) were performed using extracted bone samples. Images obtained by MXCT were further analyzed by node-strut morphometry. Results: Biochemical analysis confirmed that CRF was induced in 7/8-renal artery-ligated rats. MXCT demonstrated a significant decrease in trabecular bone in uremic rats, whereas SXA was unable to detect the difference. The node-strut method revealed decreased trabecular connectivity, with little change in the thickness of the trabecular bone in uremic rats. Conclusion: MXCT was able to detect significant changes in trabecular bone at an early stage of renal failure. The observed structural changes differed from those found in long-term dialysis patients. MXCT is a practical and promising device used for the non-invasive evaluation of early bone changes in uremic patients.