Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional and radiographic outcomes of nonoperative treatment of displaced adolescent clavicle fractures.

BACKGROUND Studies of adult patients suggest that nonoperative treatment of clavicle fractures may result in functional disability, but this has not been demonstrated in adolescents. The purpose of this study was to determine the functional outcomes after nonoperative treatment of displaced, shortened, midshaft clavicle fractures in adolescents. METHODS Adolescents ten to eighteen years of age with an isolated, completely displaced, shortened, midshaft clavicle fracture sustained between 2009 and 2011 were recruited for this study. Injury and final radiographs were assessed for displacement, shortening, and clavicle length. Maximal and endurance strength testing was performed with the Baltimore Therapeutic Equipment (BTE) machine, with use of the uninjured shoulder as an internal control. Shoulder range of motion and clavicle length were assessed clinically, and patient-oriented outcomes were obtained. RESULTS Sixteen patients (four of whom were female) with an average age (and standard deviation) of 14.2 ± 2 years and a mean duration of follow-up of 2 ± 1 years were included in the study. Fifteen patients were right-hand dominant and one was ambidextrous, and thirteen of the fractures occurred in the nondominant limb. Compared with the uninjured limb, no differences were noted in range of motion or strength except for an 8% decrease in maximal shoulder external rotation strength (p = 0.04) and a 11% loss of shoulder abduction endurance strength (p = 0.04). Radiographs demonstrated a 100% union rate but significant shortening compared with the uninjured clavicle (p ≤ 0.001). SANE (Single Assessment Numeric Evaluation), QuickDASH (shortened version of the Disabilities of the Arm, Shoulder and Hand questionnaire), and Constant scores were similar between sides. Fifteen of the sixteen patients were satisfied with the appearance of the clavicle, and all returned to full activity, including the preinjury (or a higher) level of sports participation. CONCLUSIONS Regardless of patient age, sports participation, and final clavicle shortening, no differences in pain, strength, shoulder range of motion, or subjective outcome scores were found between the injured and uninjured limbs of adolescents treated nonoperatively for a displaced, shortened, midshaft clavicle fracture.
Postoperative complications in patients with obstructive sleep apnea.

BACKGROUND Unrecognized obstructive sleep apnea (OSA) is associated with unfavorable perio-perative outcomes among patients undergoing noncardiac surgery (NCS). METHODS The study population was chosen from 39,771 patients who underwent internal medicine preoperative assessment between January 2002 and December 2006. Patients undergoing NCS within 3 years of polysomnography (PSG) were considered for the study, whereas those < 18 years of age, with a history of upper airway surgery, or who had had minor surgery under local or regional anesthesia were excluded. Patients with an apnea-hypopnea index (AHI) ≥ 5 were defined as OSA and those with an AHI < 5 as control subjects. For adjusting baseline differences in age, sex, race, BMI, type of anesthesia, American Society of Anesthesiology class, and medical comorbidities, the patients were classified into five quintiles according to a propensity score. RESULTS Out of a total of 1,759 patients who underwent both PSG and NCS, 471 met the study criteria. Of these, 282 patients had OSA, and the remaining 189 served as control subjects. The presence of OSA was associated with a higher incidence of postoperative hypoxemia (OR, 7.9; P = .009), overall complications (OR, 6.9; P = .003), and ICU transfer (OR, 4.43; P = .069), and a longer hospital length of stay (LOS), (OR, 1.65; P = .049). Neither an AHI nor use of continuous positive airway pressure at home before surgery was associated with postoperative complications (P = .3 and P = .75, respectively) or LOS (P = .97 and P = .21, respectively). CONCLUSIONS Patients with OSA are at higher risk of postoperative hypoxemia, ICU transfers, and longer hospital stay.
Complete mitochondrial genome of the blind cave barbel Sinocyclocheilus furcodorsalis (Cypriniformes: Cyprinidae)

Sinocyclocheilus furcodorsalis, a typical cavefish, has evolved some striking characters, for example loss of its eyes and reduction in melanin pigmentation, and can serve as a good model system in evolutionary adaptation developmental mechanisms. So we cloned the complete mitochondrial DNA of S. furcodorsalis (16,581 bp), which is similar to those reported from other fish mitochondrial genomes, containing 37 genes (13 protein-coding genes, 2 ribosomal RNAs, and 22 transfer RNAs) and a major noncoding control region. The complete mitogenome of the S. furcodorsalis provides an additional important data-set for the study in evolutionary adaptation developmental mechanisms.
Eye movements in reading versus nonreading tasks: Using E-Z Reader to understand the role of word/stimulus familiarity

In this paper, we extend our previous work (Reichle, Pollatsek, & Rayner, 2012) using the principles of the E-Z Reader model to examine the factors that determine when and where the eyes move in both reading and nonreading tasks, and in particular the role that word/stimulus familiarity plays in determining when the eyes move from one word/stimulus to the next. In doing this, we first provide a brief overview of E-Z Reader, including its assumption that word familiarity is the “engine” driving eye movements during reading. We then review the theoretical considerations that motivated this assumption, as well as recent empirical evidence supporting its validity. We also report the results of three new simulations that were intended to demonstrate the utility of the familiarity check in three tasks: (1) reading; (2) searching for a target word embedded in text; and (3) searching for the letter O in linear arrays of Landolt Cs. The results of these simulations suggest that the familiarity check always improves task efficiency by speeding its rate of performance. We provide several arguments as to why this conclusion is not likely to be true for the two nonreading tasks, and, in the final section of the paper, we provide a fourth simulation to test the hypothesis that problems associated with the misidentification of words may also curtail the too liberal use of word familiarity.
Infection and persistence of erythrovirus B19 in benign and cancerous thyroid tissues

Human erythrovirus B19 (EVB19) is a small, pathogenic DNA virus that has been associated with a wide range of illnesses. The primary site of replication is in bone marrow‐derived erythroid progenitor cells, but EVB19 DNA has been detected in a wide range of organs. Recently, studies have linked EVB19 to thyroid cancers and other thyroid diseases. Previous studies from multiple laboratories have detected EVB19 capsid proteins in Graves' disease, Hashimoto's thyroiditis, and thyroid cancer tissues. Data on viral gene expression and mechanism of infection in the thyroid are lacking. To investigate EVB19 infection and persistence in the thyroid, previously archived adult and pediatric tissue sections were examined for EVB19 DNA, RNA, and capsid proteins, as well as EVB19 receptor P‐antigen and co‐receptor α5β1 integrin. EVB19 DNA and protein were detected in a majority of tissues examined (87% and 68%, respectively). Detection was similar in adult and pediatric samples. Quantification of viral genomes revealed no significant difference in the amount of viral DNA in benign, cancerous, or metastatic thyroid tissues. EVB19 capsid RNA was detected in 67% of the tissues examined, confirming at least low‐level viral gene expression. Immunohistochemical staining for P‐antigen and α5β1 detected the receptor and co‐receptor most frequently on normal thyroid epithelial cells. EVB19 capsid staining could be detected in tumors lacking viral receptors. These results suggest that normal thyroid epithelial cells are the initial target for EVB19 infection in the thyroid and allow for continued persistence in both normal and cancerous thyroid tissues. J. Med. Virol. 86:1614–1620, 2014. © 2013 Wiley Periodicals, Inc.
PARylation of the forkhead‐associated domain protein DAWDLE regulates plant immunity

Protein poly(ADP‐ribosyl)ation (PARylation) primarily catalyzed by poly(ADP‐ribose) polymerases (PARPs) plays a crucial role in controlling various cellular responses. However, PARylation targets and their functions remain largely elusive. Here, we deployed an Arabidopsis protein microarray coupled with in vitro PARylation assays to globally identify PARylation targets in plants. Consistent with the essential role of PARylation in plant immunity, the forkhead‐associated (FHA) domain protein DAWDLE (DDL), one of PARP2 targets, positively regulates plant defense to both adapted and non‐adapted pathogens. Arabidopsis PARP2 interacts with and PARylates DDL, which was enhanced upon treatment of bacterial flagellin. Mass spectrometry and mutagenesis analysis identified multiple PARylation sites of DDL by PARP2. Genetic complementation assays indicate that DDL PARylation is required for its function in plant immunity. In contrast, DDL PARylation appears to be dispensable for its previously reported function in plant development partially mediated by the regulation of microRNA biogenesis. Our study uncovers many previously unknown PARylation targets and points to the distinct functions of DDL in plant immunity and development mediated by protein PARylation and small RNA biogenesis, respectively.
Acupuncture use in the United States: findings from the National Health Interview Survey.

OBJECTIVE Acupuncture has become an important provider-based complementary and alternative medicine (CAM) treatment. To improve understanding of its role in personal health care, an analysis of national data was conducted to examine user sociodemographics, conditions treated, and the relationship of use with conventional Western medical care. DESIGN A nationally representative cross-sectional survey. SETTING The 2002 National Health Interview Survey (NHIS), conducted in all 50 states and the District of Columbia. PARTICIPANTS Thirty-one-thousand and forty-four (31,044) adults who completed the NHIS Sample Adult Core. OUTCOME MEASURES The primary outcome measure was recent use of acupuncture, defined as use within the previous 12 months. RESULTS In the 2002 NHIS sample, 4.1% of the respondents reported lifetime use, and 1.1% (representing 2.13 million Americans) reported recent use of acupuncture. Recent use (n = 327) was positively associated with being an Asian female, living in the West or Northeast, having poorer self-reported health status, a higher level of education, and being an ex-smoker. Among recent users, the most typical treatment regimen was two to four treatments (34.5%), with musculoskeletal complaints being the most frequently reported conditions, led by back pain (34.0%). Reports of perceived benefit were generally high. Respondents indicated that acupuncture was used both as an alternative and as a complementary therapy. A reasonable number also reported being referred to acupuncture by a conventional medical professional (25.3%). The cross-sectional nature of the data precluded analysis of transitions in health care use (between conventional and CAM treatments) over time. CONCLUSIONS Utilization of acupuncture was somewhat lower than expected given its significant national and international recognition and its visibility in the media. This may in part be a function of provider availability and cultural factors.
Medical homes and cost and utilization among high-risk patients.

OBJECTIVES Evaluate the effects of the patient-centered medical home (PCMH) model on medical costs and utilization in the nonpediatric population, particularly among high-risk patients. STUDY DESIGN Longitudinal case-control design, comparing per member per month (PMPM) cost and utilization per 1000 patients for members enrolled in PCMH and non-PCMH practices from 2009 to 2011. METHODS Commercial health maintenance organization members in nonpediatric practices that adopted the PCMH model in 2009 were matched to patients in nonpediatric practices that did not adopt the model until 2011 or later. Propensity score matching was used to identify a pool of similar controls, and difference-in-differences regression analysis was used to compare PCMH and non-PCMH patients relative to baseline. Analysis was conducted using the complete pool of matched patients (N = 6940 cases and 6940 controls), then using the 10% of patients with the highest DxCG risk scores (N = 654 cases and 734 controls). RESULTS There were no significant cost or utilization differences for the overall population. Total cost decreased significantly more for the PCMH group than for controls in the high-risk group in years 1 and 2 (reductions of $107 and $75 PMPM), driven by lower inpatient costs. The PCMH group experienced a significantly greater reduction in inpatient admissions in all 3 years (61, 48, and 94 hospitalizations per 1000). CONCLUSIONS PCMH practices had significantly reduced costs and utilization for the highest risk patients, particularly with respect to inpatient care. As high-risk members represent a high-cost group, the most benefit can be gained by targeting these members.
Transmembrane phosphoprotein Cbp senses cell adhesion signaling mediated by Src family kinase in lipid rafts

Cbp, a C-terminal Src kinase (Csk)-binding protein, is a transmembrane phosphoprotein that has been implicated in the regulation of the Src family kinase (SFK) through recruiting Csk, a negative regulator of SFK, to a membrane microdomain of lipid rafts. To examine the contribution of Cbp to cell adhesion signaling mediated by SFK, we investigated the kinase responsible for phosphorylating Cbp and the mode of phosphorylation during the cell adhesion process. The results obtained by using mutant mice or cells that lack Csk and/or a member of SFK, Fyn, reveal that Cbp is phosphorylated predominantly by raft-localized Fyn in vivo. Upon cell adhesion onto fibronectin, Cbp becomes transiently phosphorylated (consistent with SFK activation) and recruits Csk to lipid rafts. These events are completed before the full activation of focal adhesion kinase, indicating that the transient activation and down-regulation of SFK in lipid rafts are earlier events in cell adhesion signaling. In Csk-deficient cells, continuous hyperactivation of SFK leads to continuous hyperphosphorylation of Cbp, accompanied by impaired cell spreading and migration. Silencing of Cbp by RNA interference also induced impaired cell spreading. These findings suggest that Cbp could serve as a sensor of SFK activity in early stages of cell adhesion signaling, and that Csk-mediated down-regulation of SFK is essential to allow dynamic cellular events involved in the regulation of cell spreading and migration.
Burden and quality of life of caregivers of children and adolescents with chemotherapy treatment for cancer

OBJECTIVE: To evaluate the burden of care and quality of life (QOL) of caregivers of children/adolescents with cancer during chemotherapy treatment, and to relate them to each other and sociodemographic data. METHODS: A transversal study, with 160 caregivers. We collected sociodemographic data, burden of care using the Caregiver Burden Scale and QOL using the SF-36. RESULTS: 88.7% of caregivers were mothers, with the mean age of 34.9 years, overall burden score 2.09 ± 0.04 and the SF-36 scores most compromised were: emotional aspects, vitality, mental health and physical aspects. The regression model accounted for 36.0% of the burden. CONCLUSION: The QOL of caregivers and burden of care experienced showed compromise in various domains, and these alterations may affect the quality of care provided to children and adolescents, and lead to imbalances in their own health.
Measuring Mammography and Breast Cancer Beliefs in African American Women

Although intervention trials have demonstrated significant improvement in mammography adherence for African American women, many of the current measurement tools used in these interventions have not been assessed for validity and reliability in ethnic minorities. This study assessed the validity and reliability of Health Belief Model (HBM) variables that are often the target of mammography interventions. Scale validity and reliability was assessed for HBM scales in a sample of 344 low-income African American women. Validity was supported through exploratory factor analysis and theoretical prediction of relationships. Internal consistency reliability was . 73 or above for all scales.
Risk assessment in the 21st century: Roadmap and matrix

Abstract The RISK21 integrated evaluation strategy is a problem formulation-based exposure-driven risk assessment roadmap that takes advantage of existing information to graphically represent the intersection of exposure and toxicity data on a highly visual matrix. This paper describes in detail the process for using the roadmap and matrix. The purpose of this methodology is to optimize the use of prior information and testing resources (animals, time, facilities, and personnel) to efficiently and transparently reach a risk and/or safety determination. Based on the particular problem, exposure and toxicity data should have sufficient precision to make such a decision. Estimates of exposure and toxicity, bounded by variability and/or uncertainty, are plotted on the X- and Y-axes of the RISK21 matrix, respectively. The resulting intersection is a highly visual representation of estimated risk. Decisions can then be made to increase precision in the exposure or toxicity estimates or declare that the available information is sufficient. RISK21 represents a step forward in the goal to introduce new methodologies into 21st century risk assessment. Indeed, because of its transparent and visual process, RISK21 has the potential to widen the scope of risk communication beyond those with technical expertise.
Prevention of Experimental Autoimmune Encephalomyelitis in Common Marmosets Using an Anti-IL-12p40 Monoclonal Antibody1

The experimental autoimmune encephalomyelitis (EAE) model in the common marmoset approximates recognized features of the human disease multiple sclerosis (MS) with regard to its clinical presentation as well as neuropathological and radiological aspects of the lesions in brain and spinal cord. IL-12 is a proinflammatory cytokine that is produced by APC and promotes differentiation of Th1 effector cells. IL-12 is produced in the developing lesions of patients with MS as well as in EAE-affected animals. Previously it was shown that interference in IL-12 pathways effectively prevents EAE in rodents. In this study we report that in vivo neutralization of IL-12p40 using a novel Ab has beneficial effects in the myelin-induced EAE model in common marmosets. The Ab was injected i.v. at 7-day intervals starting well after immunization (day 14) and was continued until the end of the study (day 86). Stable levels of the Ab were measured 3 days after each injection throughout the study period. During this period anti-Ab responses could not be detected. We demonstrate that anti-IL-12p40 treatment has a protective effect on the neurological dysfunction as well as on neuropathological changes normally observed in the brain and spinal cord of EAE-affected individuals.
Cancer risk among patients with cystic fibrosis and their first‐degree relatives

Patients with cystic fibrosis (CF) are at increased risk of some cancers. Little is known about the cancer risks among carriers heterozygous for the CF mutation and it is hypothesized this may be associated with reduced cancer risk. Using Swedish general population‐based registers, we identified 884 patients with CF from 1968 to 2003 and 3,033 of their first‐degree relatives The subjects were followed from birth of index persons or 1958, whichever came later, until death, emigration or 2003, whichever came first. Cancer risks were compared with the general Swedish population using standardized incidence ratios (SIR) with 95% confidence intervals (CI). Patients, followed for an average of 21 years, were at a higher overall risk of cancer. Some 26 cancer diagnoses, after excluding multiple diagnoses of nonmelanoma skin cancer in one man, produced an overall SIR of 3.2 (95% CI 2.1–4.6). We found statistically significantly increased risks for kidney, thyroid, endocrine, lymphoma and nonmelanoma skin cancer. There was no modification of cancer risk among parents and siblings, with an average of 21 years of follow‐up. This study did not identify a heterozygote advantage for CF gene mutations in relation to cancer risk. © 2009 UICC
Repartnering following relationship breakdown

T he formation of close emotional ties to a single other in an intimate relationship, particularly marriage, has been regarded as a sign of transition from adolescence to adulthood (Schwartzberg, Berliner and Jacob 1995). Yet an increasing number of young people are choosing to delay marriage or not marry at all, and an increasing number of those who do marry become single again (ABS 1997). Remarriage rates in Australia have also fallen (a decline of more than 50 per cent between 1976 and 1997), however this does not mean divorced persons are not repartnering. For the past ten years, about one-third of all marriages have involved at least one partner who has been married previously, and 20 per cent of all de facto couples involve at least one partner who is divorced – a trend that has been maintained over five years (ABS 1997). While much of the remarriage research focuses on the characteristics of those who have actually taken the step into remarriage after the breakdown of their marriage or de facto relationship, understanding the factors surrounding an individual’s preparedness to repartner can also contribute to our understanding of remarriage and repartnering trends and behaviour. The first part of this article discusses some of the research literature that has identified the characteristics of those who repartner, and the perceived benefits of repartnering. The second part reports the findings from the Australian Family Life Course Study, conducted by the Australian Institute of Family Studies, which asked unattached respondents about their future relationship intentions. Research literature
Functional connectivity associated with gait velocity during walking and walking‐while‐talking in aging: A resting‐state fMRI study

Gait decline is common among older adults and is a risk factor for adverse outcomes. Poor gait performance in dual‐task conditions, such as walking while performing a secondary cognitive interference task, is associated with increased risk of frailty, disability, and death. Yet, the functional neural substrates that support locomotion are not well established. We examined the functional connectivity associated with gait velocity in single‐ (normal pace walking) and dual‐task (walking while talking) conditions using resting‐state functional Magnetic Resonance Imaging (fMRI). We acquired 6 minutes of resting‐state fMRI data in 30 cognitively healthy older adults. Independent components analyses were performed to separate resting‐state fMRI data into group‐level statistically independent spatial components that correlated with gait velocity in single‐ and dual‐task conditions. Gait velocity in both task conditions was associated with similar functional connectivity in sensorimotor, visual, vestibular, and left fronto‐parietal cortical areas. Compared to gait velocity in the single‐task condition, the networks associated with gait velocity in the dual‐task condition were associated with greater functional connectivity in supplementary motor and prefrontal regions. Our findings show that there are partially overlapping functional networks associated with single‐ and dual‐task walking conditions. These initial findings encourage the future use of resting‐state fMRI as tool in developing a comprehensive understanding of age‐related mobility impairments. Hum Brain Mapp 36:1484–1493, 2015. © 2014 Wiley Periodicals, Inc.