Miracle-dz/UniFAR-FTUs huggingface dataset:
Antimicrobial Susceptibility of Multidrug-Resistant Acinetobacter baumanii in a Teaching Hospital: A Two-Year Observation

Multidrug-resistant (MDR) Acinetobacter baumanii (A. baumanii) caused hospital acquired infection, typically in critical-ill patients with medical devices. This is a retrospective descriptive study on epidemiology and microbiology data to determine the antimicrobial susceptibility pattern of MDR-Acinetobacter baumanii isolates from a teaching hospital in Tangerang, Indonesia from Januari 2013 to December 2014. A total of 84 A. baumanii were collected. Patients suffering from respiratory tract infection had the highest number (41.7%) of A. baumanii isolate. There were 39 (46.6%) patients admitted in critical care. A. baumanii isolates in this study mostly were multidrug-resistant organisms with low susceptibility level to 11 antibiotic tested, 44% - 69% in 2013 and 26% - 67% in 2014. A high susceptibility level was observed to amikacin (80% and 79% in 2013, 2014 consecutively) and trimethoprim-sulfamethoxazole (73% and 72% in 2013, 2014 consecutively). A. baumanii is a hospital acquired pathogen in critically-ill patients. The susceptibility pattern of this study result showed MDR organism. There was a sharp decrease of susceptibility in all antibiotics studied from 2013 to 2014 except amikacin and trimethoprim-sulfamethoxazole.
Characterization of conserved circular RNA in polyploid Gossypium species and their ancestors

Circular RNA (circRNA) is a regulatory class of long, noncoding RNA found in both plant and animal kingdoms. The profile and characterization of circRNA in cotton species remains to be explored. Here, using 24 rRNA‐depleted RNA‐seq libraries of putative diploid progenitors of Gossypium spp. , Gossypium arboreum and Gossypium raimondii, their interspecies hybrid (F1) and allotetraploid Gossypium hirsutum, 1041, 1478, 1311, and 499 circRNAs were identified in each cotton species, respectively. A prevalence of 23 exon‐circRNAs contain noncanonical GT/AG signals, and only ~ 10% of exon‐circRNA is associated with reverse complementary intronic sequences. This result implies that plants employ a method of circRNA splicing distinct from that of animals. In addition, 432 circRNAs are stably expressed in multiple cotton species.
Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets

ATG2 is one of the autophagy‐related (ATG) proteins essential for autophagosome formation and localizes to isolation membranes and lipid droplets in mammalian cells. Here, we investigated the requirement of regions in ATG2A for its organellar localization and function. The N‐terminal amino acids 1–198 and the C‐terminal amino acids 1830–1938 are required for the localization to isolation membranes and lipid droplets, respectively. The C‐terminal region is not required for the localization to isolation membranes and for autophagy. We also identified an amphipathic helix in ATG2A that is required for both its localization to organelles and autophagosome formation. These data suggest that the dual localization of ATG2A is regulated by different regions.
Effectiveness and cost of reducing particle‐related mortality with particle filtration

This study evaluates the mortality-related benefits and costs of improvements in particle filtration in U.S. homes and commercial buildings based on models with empirical inputs. The models account for time spent in various environments as well as activity levels and associated breathing rates. The scenarios evaluated include improvements in filter efficiencies in both forced-air heating and cooling systems of homes and heating, ventilating, and air conditioning systems of workplaces as well as use of portable air cleaners in homes. The predicted reductions in mortality range from approximately 0.25 to 2.4 per 10 000 population. The largest reductions in mortality were from interventions with continuously operating portable air cleaners in homes because, given our scenarios, these portable air cleaners with HEPA filters most reduced particle exposures. For some interventions, predicted annual mortality-related economic benefits exceed $1000 per person. Economic benefits always exceed costs with benefit-to-cost ratios ranging from approximately 3.9 to 133. Restricting interventions to homes of the elderly further increases the mortality reductions per unit population and the benefit-to-cost ratios.
Recent Advances in IgA Nephropathy

IgA Nephropathy: Discovery of a Distinct Glomerular Disorder (K T Woo et al.) Epidemiology and Ethnical Difference (F P Schena & F Pesce) Genetics of IgA Nephropathy (P Maxwell & Y M Wang) Histopathology, Immunofluorescence and Ultrastructural Examination (F M Lai) Tubulointerstitial Pathology (J J Roelofs & S Florquin) Podocyte Pathology (K N Lai & J C K Leung) Clinicopathologic Findings (B Julian & R Wyatt) Clinical Course (F C Berthoux & H Mohey) Special Clinical Syndromes (J Nagy & T Kovacs) Pediatric IgA Nephropathy (R Hogg) Recurrent IgA Nephropathy in Transplant (B Y Choy & K N Lai) IgA Molecule (J W Eijgenraam et al.) IgA Immune-Complex (J Novak & J Mestecky) T and B Cells (A Rifai & A Chen) IgA Receptor and Mesangial IgA Deposition (I C Mioura & R Monteiro) Antigen-Dependent Mechanism of IgA Nephropathy (Y Tomino & Y Shimizu) Complement (S C W Tang & K N Lai) Cytokines and Growth Factors (J Floege & T Ostendorf) Leukocytes, Macrophages and Monocytes (Y Ikezumi & D J Nikolic-Paterson) Renin-Angiotensin System (K N Lai & J C K Leung) Corticosteroid (C Pozzi) Renin-Angiotensin Blockage (R Coppo & A Amore) Other Immunomodulatory Agents (S C Tang & K N Lai) Other Non-immunomodulatory Agents (J Barratt & J Feehally) Tonsillectomy (O Hotta) Experimental Model of IgA Nephropathy (Y Suzuki & Y Tomino) Future Prospect for IgA Nephropathy (R J Glassock).
A new class of genic nuclear RNA species in Arabidopsis

Targeting of ArabidopsisPHABULOSA (PHB) mRNA by miR166 has been implicated in gene body methylation at the PHB locus. We report that the PHB locus produces an array of stable nuclear RNA species that are neither polyadenylated nor capped. Their biogenesis requires neither RNA polymerases IV/V nor miR166‐guided cleavage. The PHB RNAs are insensitive to mutation of nuclear RNA decay pathways and are conserved in several Brassicaceae species, suggesting functional relevance. Similar RNA species are also produced by another body‐methylated locus encoding the miR414 target eIF2. Our data reveal the existence of a new class of genic nuclear RNA species.
Survey the Quality of Life in Guilan University Students

Introduction: The Quality of Life (QOL) is the very important concept according to its mediating role in mental health in recent years. Because of the students are the future managers, investigating the QOL among them is important. Objective: Survey the QOL in Guilan University Students. Materials and Methods: In this descriptive and analytic study 226 students who were selected by multiple stage randomized cluster sampling completed WHOQOL-BREF questionnaire. Data was analyzed by t-test and pearson correlation. Results: The Findings showed that 4% of students had very good QOL, 34% had good QOL, 51% had medium level of QOL and 11% of students had low level of QOL. There was a significant difference between some dimension of QOL (medicine, somatic pain, environmental health, social care, financial resources and negative affect). Conclusion: According to the results, it suggested to measure and produce health-psychological services in various situations for improvement the level of quality of students life.
Molecular profiling and combinatorial activity of CCT068127: a potent CDK2 and CDK9 inhibitor

Deregulation of the cyclin‐dependent kinases (CDKs) has been implicated in the pathogenesis of multiple cancer types. Consequently, CDKs have garnered intense interest as therapeutic targets for the treatment of cancer. We describe herein the molecular and cellular effects of CCT068127, a novel inhibitor of CDK2 and CDK9. Optimized from the purine template of seliciclib, CCT068127 exhibits greater potency and selectivity against purified CDK2 and CDK9 and superior antiproliferative activity against human colon cancer and melanoma cell lines. X‐ray crystallography studies reveal that hydrogen bonding with the DFG motif of CDK2 is the likely mechanism of greater enzymatic potency. Commensurate with inhibition of CDK activity, CCT068127 treatment results in decreased retinoblastoma protein (RB) phosphorylation, reduced phosphorylation of RNA polymerase II, and induction of cell cycle arrest and apoptosis. The transcriptional signature of CCT068127 shows greatest similarity to other small‐molecule CDK and also HDAC inhibitors. CCT068127 caused a dramatic loss in expression of DUSP6 phosphatase, alongside elevated ERK phosphorylation and activation of MAPK pathway target genes. MCL1 protein levels are rapidly decreased by CCT068127 treatment and this associates with synergistic antiproliferative activity after combined treatment with CCT068127 and ABT263, a BCL2 family inhibitor. These findings support the rational combination of this series of CDK2/9 inhibitors and BCL2 family inhibitors for the treatment of human cancer.
Malaria in immigrant and traveler children: Clinical presentation and risk factors for complications

Abstract To review the clinical features and risk factors for complications and treatment of malaria in a pediatric cohort mainly composed of immigrant children. Retrospective cohort study of all cases of malaria diagnosed between 1991 and 2001 in Montreal, Canada. We have reviewed a total of 121 cases of malaria in children. The majority of which (78%) were immigrants or foreign-born visitors. Most children were originating from African countries and mainly infected with Plasmodium falciparum (63.9%, versus 21.0%, 5.9%, 3.4% for Plasmodium vivax), Plasmodium ovale and Plasmodium malariae respectively). Seven patients (5.9%) were co-infected with more than one species. Median times from arrival to presentation were much shorter with P. falciparum (14 days) than other species: P. vivax (98 days), P. ovale (37 days) and P. malariae (31 days). The majority of children had fever, headache and rigors. Ten children (8.3%) had serious complications (cerebral malaria, anemia or shock). All ten patients presented within one month of arrival from their respective endemic country and all were infected with P. falciparum (P = 0.03). Three patients required admission to a pediatric intensive care unit and seven required a blood transfusion. All recovered well although two children had P. falciparum recrudescence and three children infected with P. vivax or P. ovale relapsed. The number of complications observed in immigrant children was similar to that observed in travelers. Sickle cell disease was the most significant associated risk factor for serious complications of malaria (P < 0.001). Immigrant children are at high risk of complications from malaria. Our study highlights sickle cell disease as a significant risk factor for serious complications of malaria in these children. It is essential to maintain a high index of suspicion in non-endemic areas in order to decrease the morbidity of malaria in immigrant children.
Poisson regression model in ePidemiology - an introduction

The device can be applied to vehicles provided with an electronic control device for the vehicle engine. The protecting device for includes a keyboard for entering a user's code, a microprocessor for converting entered data coming from the keyboard into an output message containing the user's code, programmable and non-volatile memory for storing the code for unlocking the engine electronic control device, a microprocessor for comparing the unlocking code stored in the memory with the user's code contained in said message coming from the keyboard and unlocking the engine electronic control device when there is coincidence of the code contained in the memory with that contained in said output message, and the microprocessor and the memory being capable of effecting replacement of the unlocking code of the engine control device with a new unlocking code through action on the keyboard.
microRNA‐497 inhibits cell proliferation and induces apoptosis by targeting YAP1 in human hepatocellular carcinoma

microRNAs (miRNAs) function as oncogenes or tumor suppressors in human cancers by targeting mRNAs for degradation and/or translational repression. miR‐497 has been proposed as a tumor suppressive miRNA and its deregulation is observed in human cancers. However, the prognostic value of miR‐497 and its underlying molecular pathways involved in the initiation and development of hepatocellular carcinoma (HCC) are poorly investigated. In the present study, we found that the mean level of miR‐497 in HCC tissues was lower than that in adjacent nontumor tissues. Clinical data indicated that low expression of miR‐497 was prominently associated with adverse prognostic features of HCC including high serum alpha‐fetoprotein (AFP) level, large tumor size, high Edmondson–Steiner grading and advanced tumor–node–metastasis (TNM) stage. Furthermore, miR‐497 was an independent prognostic factor for indicating both 5‐year overall survival and disease‐free survival of HCC patients. Gain‐ and loss‐of‐function studies showed that miR‐497 reduced cell proliferation and induced apoptosis in HCC cells. Yes‐associated protein 1 (YAP1) was identified as a direct target of miR‐497 in HCC. An inverse correlation between YAP1 and miR‐497 expression was observed in HCC tissues. Notably, YAP1 knockdown abrogated the effects of miR‐497 deletion on HCC cells with decreased cell proliferation and increased apoptosis. In conclusion, we report that miR‐497 is a potent prognostic indicator and may suppress tumor growth of HCC by targeting YAP1.
Research methods for graduate students: A practical framework to guide teachers and learners

Purpose: The purpose of this article is to present the Arrow Framework for Research Design, an organizing framework that facilitates teaching and learning of research methods, providing logical organization of interrelationships between concepts, content, and context of research methods, and practice application. The Arrow Framework was designed for teaching and learning research methods to facilitate progression of knowledge acquisition through synthesis. Data sources: The framework was developed over several years and used successfully to teach masters, DNP, and PhD nursing students across five universities. The framework is presented with incremental graphics and narrative for teaching. Conclusion: The Arrow Framework provides user‐friendly information, in an organized and systematic approach demonstrated as successful for teaching and learning the foundational language of research, facilitating synthesis and application in scholarly endeavors. Implications for practice: The Arrow Framework will be useful for educators and students in teaching and learning research language, relationships, and application of methods. The materials are easily adaptable to slide or paper presentation, and meet learner needs for narrative and visual presentation. Teaching research design to graduate students is critical to meet the expectation that students are to understand the scientific underpinnings of nursing science and appropriate use of evidence that are essential for well‐educated practitioners.
Quality of Nursing Care Provided for Neonates with Tracheoesophageal Fistula

Tracheoesophageal fistula (TEF) is an abnormal connection between the esophagus and the trachea. It is one of the most life-threatening anomalies in newborn. The current study aimed to assess the quality of nursing care provided for neonates with TEF and to assess the nurses’ knowledge and performance regarding neonates with TEF. A descriptive exploratory research design was utilized in the current study. The study was conducted at the Neonatal Surgical Intensive Care Unit (NSICU) affiliated to Benha Specialized Pediatric Hospital. A convenient sample composed of all nurses working at NSICU and all neonates with TEF within six months from starting study. Data were collected using a structured questionnaire sheet to assess nurses’ knowledge regarding quality of nursing care for neonates with TEF and observational checklists to assess nurses’ performance for neonates with TEF at NSICU. The study results evident that three quarters of nurse shad average level of knowledge regarding neonates with TEF and all of them had good level of performance provided for neonates with TEF. Meanwhile, there were positive correlation between nurses’ knowledge and performance scores. The present study concluded that, there were positive correlation between nurses’ knowledge and performance scores in relation to their socio-demographic characteristics. It was recommended that periodical educational training programs for nurses working at NSICU are mandatory, for the purpose of updating the knowledge and to maintain efficient performance and to reach high quality of care. Key words: Neonate-Tracheoesophageal Fistula-Quality of nursing care
Predicting psychological distress of informal carers of individuals with major depression or bipolar disorder

Caring for someone with a mental illness is associated with high levels of burden and psychological distress. Understanding these factors could be important to prevent the development of physical and mental health problems in carers. The purpose of the present study was to determine the contribution of coping styles and social support in predicting the psychological distress reported by informal carers (IC) of individuals with major depression or bipolar disorder. IC (n = 72) of adults with a diagnosed depressive illness were recruited from mental health organizations within the community setting. Carers completed the General Health Questionnaire, Brief COPE, and Social Support Questionnaire. Multiple linear regression analysis revealed that 63% of the variance in psychological distress could be accounted for by adaptive and maladaptive coping styles and perceived quantity and satisfaction with social support. Satisfaction with social support (β = -0.508, P < 0.001) and maladaptive coping (β = 0.369; P < 0.001) were significant predictors of psychological distress. These findings suggest that interventions should consider coping styles, specifically the interaction between maladaptive behaviours and enhancement of quality of support, to assist carers to manage psychological distress, especially earlier in the caring role.
Precepting nurse practitioner students: One medical center's efforts to improve the precepting process

Purpose:To describe the challenges shared by schools of nursing and precepting institutions in meeting the clinical rotation requirements for nurse practitioner (NP) students. A formal process was developed to screen students and provide appropriate clinical placement with ongoing evaluations. Detailed description of a preceptor class for NPs is offered. Data sources:Single institution NP survey, preceptor class participant survey, author experience, and PubMed, CINAHL. Conclusions:The barriers to precepting are well known, particularly from the viewpoint of schools of nursing. This article describes the barriers from a precepting institution's standpoint. Standardizing processes and expectations for NP students and preceptors improves the overall efficiency, effectiveness, and safety of the clinical experiences. Implications for practice:Highlights of an NP preceptor class and efforts to streamline precepting arrangements are described. Lessons learned, including the need to minimize paperwork burden on students during the preclinical period and the continued need for improvement of evaluation tools, are discussed. Recommendations for ways to blend specialty rotations with primary care rotations are made.
Why didn't I know? Perspectives from adult children of elderly parents with dementia

PURPOSE Approximately 27 million adults are living with undiagnosed dementia worldwide. The purpose of this study was to learn about the experiences of adult children of elderly parents who were ultimately diagnosed with dementia. DATA SOURCES A descriptive qualitative design was used for identifying if there were prodromal signs or symptoms that preceded the more commonly known warning signs of dementia. Twelve, 60-min interviews were conducted over the telephone. CONCLUSIONS The overarching theme was: "Why didn't I know? " Subthemes were "We ignored it because we didn't want it to be," "We thought it was grief," and "They wouldn't diagnose dementia. " Normal aging changes often mask the identification of mild cognitive impairment (MCI) and dementia. Healthcare providers should screen patients for dementia when cognitive concerns or changes become apparent. IMPLICATIONS FOR PRACTICE The General Practitioner Assessment of Cognition and the Mini-Cog can be used for screening cognitive changes. Since depression may be misinterpreted as dementia, screening for depression should also be included if there is a potential for overlap. This is important in those patients who are experiencing grief, as many signs and symptoms of early grief are the same as early dementia.
Limited scientific evidence supports the use of conservative treatment interventions for pain and function in patients with subacromial impingement syndrome: randomized control trials

Abstract Background: Different conservative treatment interventions are often used to decrease pain and improve function in patients with subacromial impingement syndrome (SAIS). However, the current evidence to support the use of these interventions has not been established. Objectives: To determine the evidence for conservative treatment interventions regarding pain and function compared to any intervention for SAIS patients. Methods: A systematic review of randomized controlled trials, published in English between 1 January 1999 and 31 May 2010, was performed using PubMed, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Cochrane Library and manual searching. The methodological quality (PEDro scale) and evidence grade (SBU) were rated. Eight studies were of high quality, four were medium quality, and eight were low quality. Various conservative treatment interventions were evaluated: acupuncture, electrotherapy modalities, exercises, mixed modalities, changing posture, and use of a functional brace. Results: Limited scientific evidence (LSE) indicates positive effects of exercise and mixed modalities regarding pain and function and high-intensity laser therapy (HILT) regarding pain in SAIS patients. LSE also indicate no effect of electrotherapy modalities, apart from HILT, as treatment for SAIS. Conclusions: The lack of high quality interventions limits the ability to draw conclusions regarding efficacy from several of the included studies. However, exercise may be as efficient as surgery, manual therapy in combination with exercise seems to be more effective than exercise alone and high-dose exercises seem to be more effective than low-dose exercises. Furthermore, there is contradictory evidence to support the use of acupuncture.