Miracle-dz/UniFAR-FTUs huggingface dataset:
A Comparison of Acute Toxicity Endpoints for Adult Honey Bees with Technical Grade Active Ingredients and Typical End-use Products as Test Substance

Abstract The honey bee, Apis mellifera L. (Hymenoptera: Apidae), is a model organism for pollinators in risk assessment frameworks globally.The acute toxicity tests with adult honey bees for contact and oral exposure are part of the requirements for pesticide registration and are typically conducted with the active ingredient. A question often asked is if the typical end-use product (TEP) is more toxic than the technical grade active ingredient (TGAI) to honey bees. We explored this question by mining publicly available databases from regulatory agencies worldwide, where testing with theTEP is required.The objective of this study was to determine whetherTEPs are comparable in toxicity to the TGAI. The dataset was analyzed via a 3 × 3 contingency table with toxicity categories, as the data cannot be computed for regression analysis. Of the 151 active ingredients with reported endpoints for contact exposure, 28 were classified as either moderately or highly toxic, 123 were classified as practically nontoxic, and 3 were inconclusive. Only two (1.3%) were reclassified from nontoxic to moderately toxic as theTEP. Of the 141 active ingredients with reported endpoints for oral exposure, 23 were classified as moderately or highly toxic, 113 were classified as practically nontoxic, and 5 were inconclusive. Only five (3.6%) were reclassified from nontoxic to moderately toxic as theTEP. Fewer than 5% of the totalTEPs evaluated (contact and oral) were shown to be more toxic than theTGAI, suggesting that the risk assessments ofTGAIs would be sufficiently protective to pollinators at the screening laboratory level.
Initial Studies with 11C-Vorozole PET Detect Overexpression of Intratumoral Aromatase in Breast Cancer

Aromatase inhibitors are the mainstay of hormonal therapy in estrogen receptor–positive breast cancer, although the response rate is just over 50% and in vitro studies suggest that only two thirds of postmenopausal breast tumors overexpress aromatase. The goal of the present study was to validate and optimize PET with 11C-vorozole for measuring aromatase expression in postmenopausal breast cancer in vivo. Methods: Ten newly diagnosed postmenopausal women with biopsy-confirmed breast cancer were administered 11C-vorozole intravenously, and PET emission data were collected between 40 and 90 min after injection. Tracer injection and scanning were repeated 2 h after ingestion of 2.5 mg of letrozole. Mean and maximal SUVs and ratios to nontumor tissue in the contralateral breast were determined at baseline and after letrozole. Biopsy specimens from the same tumors were stained for aromatase using immunohistochemistry and evaluated for stain intensity and the percentage of immune-positive cells. Results: Seven of the 10 women (70%) demonstrated increased mean focal uptake of tracer (SUV ratio > 1.1) coinciding with the mammographic location of the lesion, whereas the other 3 women (30%) did not (SUV ratio ≤ 1.0). All patients with an SUV ratio above 1.1 had mean SUVs above 2.4, and there was no overlap (SUV ratio ≤ 1; SUVmean, 0.8–1.8). The SUV ratio relative to breast around tumor was indistinguishable from the ratio to contralateral breast. Pretreatment with letrozole reduced tracer uptake in most subjects, although the percentage of blocking varied across and within tumors. Tumors with a high SUV in vivo also showed a high immunohistochemical staining intensity. Conclusion: PET with 11C-vorozole is a useful technique for measuring aromatase expression in individual breast lesions, enabling noninvasive quantitative measurement of baseline and posttreatment aromatase availability in primary tumors and metastatic lesions.
Conservation and global distribution of non-canonical antigens in Enterotoxigenic Escherichia coli

Background Enterotoxigenic Escherichia coli (ETEC) cause significant diarrheal morbidity and mortality in children of resource-limited regions, warranting development of effective vaccine strategies. Genetic diversity of the ETEC pathovar has impeded development of broadly protective vaccines centered on the classical canonical antigens, the colonization factors and heat-labile toxin. Two non-canonical ETEC antigens, the EtpA adhesin, and the EatA mucinase are immunogenic in humans and protective in animal models. To foster rational vaccine design that complements existing strategies, we examined the distribution and molecular conservation of these antigens in a diverse population of ETEC isolates. Methods Geographically diverse ETEC isolates (n = 1159) were interrogated by PCR, immunoblotting, and/or whole genome sequencing (n = 46) to examine antigen conservation. The most divergent proteins were purified and their core functions assessed in vitro. Results EatA and EtpA or their coding sequences were present in 57.0% and 51.5% of the ETEC isolates overall, respectively; and were globally dispersed without significant regional differences in antigen distribution. These antigens also exhibited >93% amino acid sequence identity with even the most divergent proteins retaining the core adhesin and mucinase activity assigned to the prototype molecules. Conclusions EtpA and EatA are well-conserved molecules in the ETEC pathovar, suggesting that they serve important roles in virulence and that they could be exploited for rational vaccine design.
Genome-Wide Analyses Revealed Remarkable Heterogeneity in Pathogenicity Determinants, Antimicrobial Compounds, and CRISPR-Cas Systems of Complex Phytopathogenic Genus Pectobacterium

The Pectobacterium genus comprises pectolytic enterobacteria defined as the causal agents of soft rot, blackleg, and aerial stem rot diseases of potato and economically important crops. In this study, we undertook extensive genome-wide comparative analyses of twelve species that conform the Pectobacterium genus. Bioinformatics approaches outlined a low nucleotide identity of P. parmentieri and P. wasabiae with other species, while P. carotovorum subsp. odoriferum was shown to harbor numerous pseudogenes, which suggests low coding capacity and genomic degradation. The genome atlases allowed for distinguishing distinct DNA structures and highlighted suspicious high transcription zones. The analyses unveiled a noteworthy heterogeneity in the pathogenicity determinants. Specifically, phytotoxins, polysaccharides, iron uptake systems, and the type secretion systems III–V were observed in just some species. Likewise, a comparison of gene clusters encoding antimicrobial compounds put in evidence for high conservation of carotovoricin, whereas a few species possessed the phenazine, carbapenem, and carocins. Moreover, three clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) systems: I-E, I-F, and III-A were identified. Surrounding some CRISPR-Cas regions, different toxin and antitoxin systems were found, which suggests bacterial suicide in the case of an immune system failure. Multiple whole-genome alignments shed light on to the presence of a novel cellobiose phosphotransferase system (PTS) exclusive to P. parmenteri, and an unreported T5SS conserved in almost all species. Several regions that were associated with virulence, microbe antagonism, and adaptive immune systems were predicted within genomic islands, which underscored the essential role that horizontal gene transfer has imparted in the dynamic evolution and speciation of Pectobacterium species. Overall, the results decipher the different strategies that each species has developed to infect their hosts, outcompete for food resources, and defend against bacteriophages. Our investigation provides novel genetic insights that will assist in understanding the pathogenic lifestyle of Pectobacterium, a genus that jeopardizes the agriculture sustainability of important crops worldwide.
Treatment outcome with orthodontic aligners and fixed appliances: a systematic review with meta-analyses.

BACKGROUND The use of orthodontic aligners to treat a variety of malocclusions has seen considerable increase in the last years, yet evidence about their efficacy and adverse effects relative to conventional fixed orthodontic appliances remains unclear. OBJECTIVE This systematic review assesses the efficacy of aligners and fixed appliances for comprehensive orthodontic treatment. SEARCH METHODS Eight databases were searched without limitations in April 2019. SELECTION CRITERIA Randomized or matched non-randomized studies. DATA COLLECTION AND ANALYSIS Study selection, data extraction, and risk of bias assessment was done independently in triplicate. Random-effects meta-analyses of mean differences (MDs) or relative risks (RRs) with their 95% confidence intervals (CIs) were conducted, followed by sensitivity analyses, and the GRADE analysis of the evidence quality. RESULTS A total of 11 studies (4 randomized/7 non-randomized) were included comparing aligners with braces (887 patients; mean age 28.0 years; 33% male). Moderate quality evidence indicated that treatment with orthodontic aligners is associated with worse occlusal outcome with the American Board of Orthodontics Objective Grading System (3 studies; MD = 9.9; 95% CI = 3.6-16.2) and more patients with unacceptable results (3 studies; RR = 1.6; 95% CI = 1.2-2.0). No significant differences were seen for treatment duration. The main limitations of existing evidence pertained to risk of bias, inconsistency, and imprecision of included studies. CONCLUSIONS Orthodontic treatment with aligners is associated with worse treatment outcome compared to fixed appliances in adult patients. Current evidence does not support the clinical use of aligners as a treatment modality that is equally effective to the gold standard of braces. REGISTRATION PROSPERO (CRD42019131589).
1, 25 Dihydroxyvitamin D3 Protects the Heart Against Pressure Overload-induced Hypertrophy without Affecting SIRT1 mRNA Level

Background and Aims: There has been scant information concerning antihypertrophic effects of vitamin D specifically on its cellular and molecular mechanisms. Sirtuin 1 (SIRT1) is regarded as a key deacetylase enzyme in cardiomyocytes which applies potential cardioprotective effects by functional regulation of different proteins. This study aimed to evaluate the effects of 1, 25-dihydroxyvitamin D on the hypertrophic markers and cardiac level of SIRT1 mRNA in rats following the aortic banding. Material and Methods: In this study, male Wistar rats (170-220g) were used, which were divided into 4 groups: rats subjected to hypertrophy without treatment (H), rats pretreated with 1,25 dihydroxyvitamin D3 (H+VD), rats received propyleneglycol as a vitamin solvent (H+P), and intact animals which were elected as the control group. Arterial blood pressure was directly measured by the carotid cannulation. Transcription level of target genes was measured by real time polymerase chain reaction technique. Results: In H+VD group, systolic blood pressure as well as heart weight-to-body weight ratio decreased significantly compared to the group H (P<0.01). Moreover, regarding hypertrophy marker genes in H+VD group, both atrial natriuretic peptide mRNA (H+VD:64.8±14% vs. H:127±26%; P<0.05) and brain natriuretic peptide mRNA (H+VD:25.6±6% vs. H:84.2±12%; P<0.01) levels decreased significantly. SIRT1 mRNA level was increased by 56.8±14% in group H and by 42.6±12% in group H+VD which were significant in comparison to the control group (P<0.01 and P<0.05, respectively). No significant difference was noted between H+VD and H groups. Conclusions: The results of the present study revealed that administration of 1, 25dihydroxyvitamin D decreases myocardial hypertrophy markers in rats following the abdominal aortic banding. The pressure overload-induced hypertrophy accompanies with SIRT1 mRNA upregulation, though antihypertrophic effects of vitamin didnot participate in SIRT1 transcription level.
Controlled Growth of BiSI Nanorod-Based Films through a Two-Step Solution Process for Solar Cell Applications

Pb-based hybrid perovskite solar cells, despite their advantages, face challenges in commercialization. In recent years, Bi-based chalcohalides are being considered as potential alternative candidates, however, their current device efficiency remains unsatisfactory. Herein, a two-step solution method is developed and applied to the fabrication of BiSI films. The method consists of the formation of Bi2S3 (step I) and its conversion to BiSI (step II). The Bi2S3 was fabricated by a thiol-amine solution process and the BiSI conversion was achieved by chemical reaction between the as-formed Bi2S3 and BiI3. It was found that the formation of BiSI was highly dependent on the Bi:S molar ratio of the Bi2O3-thiourea solution and the number of times of step I. The as-fabricated BiSI film had an optical band gap of 1.61 eV and exhibited nanorod morphology. In addition, the electronic structure is explored and discussed for solar cells applications.
Prevalence and determinants of co-use of alcohol and tobacco among men in working age group (18-59 years) in India

Background: The relationship between tobacco and alcohol use is very important in making strategies containing use of these products. Alcohol and tobacco use were studied separately in Indian studies, which can undermine their co-occurrences. The objective of the study is to know the prevalence and socio-economic determinants of co-occurrence of alcohol and tobacco use among men in working age groups. Data Sources: The data from the Study on Global Ageing and Adult Health (SAGE) Wave1 was used. It was a cross sectional household survey, which collected information for adults aged 18 years and above. Results: The prevalence of co-use of tobacco and alcohol among men aged 18-59 years was 23.5%. High use of tobacco was found among alcohol user. Age cohorts were also the important determinants of alcohol and tobacco use. The inverted U or V-shape relation was found between age groups and co-use of tobacco and alcohol. The prevalence was lowest for age groups 18-24 years and 50-59 years. Low education attainment and working in informal sector were the main risk factors for high co-use of these substances among men. The study also tried to look at the relationship between the co-use of alcohol and tobacco with some selected health indicators. Use of alcohol and tobacco was significantly related with under nutrition and increasing blood pressure levels among men in India. Conclusion: To contain the use of alcohol and tobacco to improve heath condition among adult men, policies and strategies must be form keeping the importance of their co-occurrences.
Dysfunction of the corticostriatal pathway in autism spectrum disorders

The corticostriatal pathway that carries sensory, motor, and limbic information to the striatum plays a critical role in motor control, action selection, and reward. Dysfunction of this pathway is associated with many neurological and psychiatric disorders. Corticostriatal synapses have unique features in their cortical origins and striatal targets. In this review, we first describe axonal growth and synaptogenesis in the corticostriatal pathway during development, and then summarize the current understanding of the molecular bases of synaptic transmission and plasticity at mature corticostriatal synapses. Genes associated with autism spectrum disorder (ASD) have been implicated in axonal growth abnormalities, imbalance of the synaptic excitation/inhibition ratio, and altered long‐term synaptic plasticity in the corticostriatal pathway. Here, we review a number of ASD‐associated high‐confidence genes, including FMR1, KMT2A, GRIN2B, SCN2A, NLGN1, NLGN3, MET, CNTNAP2, FOXP2, TSHZ3, SHANK3, PTEN, CHD8, MECP2, DYRK1A, RELN, FOXP1, SYNGAP1, and NRXN, and discuss their relevance to proper corticostriatal function.
Family history of cancer as a cue to action for physical activity behaviour and beliefs

Abstract Objective: Examine if family history of cancer serves as a cue to action prompting adults to assess their personal risk of cancer, consider the consequences, and engage in physical activity (PA) if they believe it will reduce their risk. Design: Cross-sectional survey of adults with and without a close relative with cancer. Main Outcome Measures: Health Belief Model (HBM) constructs of perceived cancer vulnerability, perceived cancer severity, response effectiveness of PA, self-efficacy for PA, and barriers to PA, and moderate-to-vigorous intensity PA (MVPA). Results: Perceived barriers and self-efficacy correlated with MVPA in both groups (p < .05), and perceived vulnerability and response effectiveness correlated with MVPA in participants with a close relative with cancer (p < .05). In multiple regression analyses, HBM constructs accounted for 18.5% and 8.3% of the variability in MVPA among participants with and without a close relative with cancer, respectively. Participants with a close relative with cancer had greater perceived vulnerability to cancer compared to participants without a close relative with cancer (p < .001). Conclusion: Adults with and without a close relative with cancer may be differentially influenced by HBM constructs, and as a result may respond differently to PA interventions and public health messaging.
Hospital and Patient Characteristics Regarding the Place of Death of Hospitalized Impending Death Patients: A Multilevel Analysis

Objectives: To explore the influence of hospital and patient characteristics on deaths at home among inpatients facing impending death. Method: In this historical cohort study, 95,626 inpatients facing impending death from 362 hospitals in 2011 were recruited. The dependent variable was the place of death. The independent variables were the characteristics of the hospitals and the patients. A two-level hierarchical generalized linear model was used. Results: In total, 41.06% of subjects died at home. The hospital characteristics contributed to 29.25% of the total variation of the place of death. Private hospitals (odds ratio [OR] = 1.32, 95% confidence interval [CI] = 1.00–1.75), patients >65 years old (OR = 1.48, 95% CI. = 1.42–1.54), married (OR = 3.15, 95% CI. = 2.93–3.40) or widowed (OR = 3.39, 95% CI. = 3.12–3.67), from near-poor households (OR = 5.16, 95% CI. = 4.57–5.84), having diabetes mellitus (OR = 1.79, 95% CI. = 1.65–1.94), and living in a subcounty (OR = 2.27, 95% CI. = 2.16–2.38) were all risk factors for a death at home. Conclusion: Both hospital and patient characteristics have an effect of deaths at home among inpatients facing impending death. The value of the inpatient mortality rate as a major index of hospital accreditation should be interpreted intrinsically with the rate of deaths at home.
Examining the Factor Structure of the Pittsburgh Sleep Quality Index in a Multi-Ethnic Working Population in Singapore

The Pittsburgh Sleep Quality Index (PSQI) is a widely used measure for assessing sleep impairment. Although it was developed as a unidimensional instrument, there is much debate that it contains multidimensional latent constructs. This study aims to investigate the dimensionality of the underlying factor structure of the PSQI in a multi-ethnic working population in Singapore. The PSQI was administered on three occasions (baseline, 3 months and 12 months) to full-time employees participating in a workplace cohort study. Exploratory factor analysis (EFA) investigated the latent factor structure of the scale at each timepoint. Confirmatory factor analysis (CFA) evaluated the model identified by EFA, and additionally evaluated it against a single factor and a three-factor model. The EFA identified a two-factor model with similar internal consistency and goodness-of-fit across each timepoint. In the CFA, the two- and three-factor models were both superior to the unidimensional model. The two- and three-factor models of the PSQI were reliable, consistent and provided similar goodness-of-fit over time, and both models were superior to the unidimensional measure. We recommend using the two-factor model to assess sleep characteristics in working populations in Singapore, given that it performs as well as the three-factor model and is simpler compared to the latter.
COPD_A_217317 2423..2431

Robert L Clancy 1 Allan W Cripps 2 1School of Medicine and Public Health, University of Newcastle, Newcastle, NSW, Australia; 2School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Gold Coast, QLD, Australia Abstract: In subjects with chronic bronchitis, protection against acute bronchitis following oral administration of a whole-cell killed nontypeable Haemophilus influenzae (NTHi) preparation was demonstrated in the mid-1980s. Subsequently, studies aiming to validate clinical efficacy of this oral treatment were complicated by a number of factors, including the modification of clinical definitions, the implications of which were not recognized at that time. The objective of this review is to integrate our pre-clinical and clinical research in this field conducted over the past 30 years to demonstrate the evolution of the idea of communication between mucosal surfaces through the common mucosal immune system and the development of an effective oral NTHi immunotherapy. Our earliest studies recruited subjects with chronic sputum production and high levels of culture-positive sputum for Gram-negative bacteria but by 2000, the clinical diagnostic focus had switched from “chronic bronchitis” to “chronic obstructive pulmonary disease” (COPD), which was functionally defined using spirometry. This change led to variable clinical trial results, confirming the importance of chronic sputum production and culturepositive sputum. Additional conditioning factors such as patient age and gender were influential in study populations with low culture-positive sputum production. Through this period, studies in human and in rodent models provided new insights into airway protection mechanisms and the pathogenesis of airway inflammation. Key findings were the importance of a dysbiosis within the airwaymicrobiome, and the critical role of an interdependence between the bronchus and the gut, with a Peyer’s patch-dependent extra-bronchus “loop” controlling the composition of the bronchus microbiome. Within this context, intercurrent virus infections initiate a microbiomedependant hypersensitivity reaction involving Peyer’s patch-derived Th17 cells. We conclude that whole-cell killed NTHi immunotherapy has consistent and significant benefits when examined in the context of changing clinical disease definitions, age and gender, and has the potential to change the natural history of chronic airway disease.
Research on Constructing the Teaching System of Physical Education Theory Course in Colleges and Universities

This study centers on the thoughts, guidance, plans and learning habits of University P.E. professors. According to the complete investigation, it is very important to explore and establish a physical education system, aiming at improving the quality of physical education and students'enthusiasm for physical education learning. Based on the analysis of the professors of sports theory in China in the past ten years, this paper makes a study. Focus is on sports meeting, sports aesthetics, Health Science and psychological theory. From the point of view of appreciating sports, sports competition is the starting point. Promoting health and enhancing scientific exercise methods is one of the ways to enhance physical strength. The core content system of sports theory education has been constructed innovatively. Data show that short-term universities and target universities basically follow the basic conditions of “outline” to achieve their goals. At the same time, it has a good reference value for promoting students'good way of thinking and the formation of other university sports theory education.