Miracle-dz/UniFAR-FTUs huggingface dataset:
Isolated heart model demonstrates evidence of contractile and diastolic dysfunction in right ventricles from rats with sugen/hypoxia‐induced pulmonary hypertension

Although extensively used for the study of left ventricular function, limited experience exists with the isolated heart model in the evaluation of right ventricular (RV) function. In particular, no published experience exists with this tool in sugen/hypoxia‐induced pulmonary hypertension (SuHx‐PH), a frequently used model of severe and progressive PH. We sought to characterize markers of RV contractile and diastolic function in SuHx‐PH and to establish their relationship with markers of maladaptive RV remodeling. Hearts were excised from anesthetized Sprague Dawley rats with or without SuHx‐PH and perfused via the aorta using a Langendorff preparation. We explored the Frank–Starling relationship of RV function (RV developed pressure, dP/dtmax, and dP/dtmin; all normalized to RV mass) by increasing RV end‐diastolic pressure (RVEDP) from 0 to 40 mmHg. Functional studies were complemented by quantification of RV pro‐apoptotic signaling (bcl2/bax), procontractile signaling (apelin), and stress response signaling (p38MAPK activation). Pearson's correlation analysis was performed for functional and biochemical parameters. SuHx‐RVs exhibited severe RV dysfunction with marked hypertrophy and decreased echocardiographic cardiac output. For any given RVEDP, SuHx‐RVs demonstrated less developed pressure and lower dP/dtmax, as well as less pronounced dP/dtmin, suggestive of decreased contractile and diastolic function. SuHx‐RVs exhibited decreased bcl2/bax ratios, apelin expression, and p38MAPK activation. Bcl2/bax and apelin RNA abundance correlated positively with RV developed pressure and dP/dtmax and negatively with dP/dtmin. p38MAPK activation correlated positively with RV developed pressure. We conclude that SuHx‐RVs exhibit severe contractile and diastolic dysfunction. Increased pro‐apoptotic signaling and attenuated procontractile and stress response signaling may contribute to these functional alterations.
Effect of DGAT1 gene variants on milk quantity and quality in Holstein, Simmental and Brown Swiss cattle breeds in Croatia

The aim of this study was to determine allele frequency at the diacylglycerol-O-transferase 1 gene (DGAT1) K232A polymorphic site and effect of allelic variants K and A on milk production traits of Holstein (dairy), Simmental and Brown Swiss (dual purpose) cattle breeds in Croatia. The DNA was extracted from hair of 371 cows, while coding region of DGAT1p.K232A was amplified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Dominant frequency of K allele was determined in all three analysed breeds: Holstein (0.775), Simmental (0.616) and Brown Swiss (0.651). The most Holstein cows were homozygotus for KK variant (0.637), while dominant genotype in other two breeds was KA (Simmental 0.647; Brown Swiss 0.644). Positive effect of KK genotype on milk yield was detected in Holstein and Brown Swiss breeds, while in Simmental breed was negative (-220.8 kg; -463.6 kg) in regard to KA and AA genotype. Milk of cows with KK genotype in Holstein breed, KA in Simmental and AA in Brown Swiss were found as the most favourable in terms of fat content. Cows of AA genotype in all the three analysed breeds have had higher milk protein content. Statistical analysis was performed using the GLM procedure, including genotype and breed effects as independent variables. The effects of DGAT1genotypes on milk quantity and quality were not significant (P>0.05).
Polyphenolic Compounds and Free Radical Scavenging Activity in Eight Lamiaceae Herbs of Manipur

Eight plants of Lamiaceae under subfamily Nepetoideae found in Manipur, India were selected for estimation of their polyphenolic compounds and free radical scavenging activity which is expressed on dry weight basis. In this present study, the total phenol and flavonoid contents as well as the free radical scavenging activity were studied using spectrophotometric method. The total phenol content was determined based on Folin-Ciocalteau reagent, flavonoid was determined by aluminium chloride spectrophotometric method and tannin by Folin Dennis Method. The free radical scavenging activity was determined by using DPPH radical which is expressed as IC 50 (μg/ml). The total phenolic content varied from 21.39 ± 0.927 to 46.28 ± 0.543 mg/g, flavonoids content in the selected samples varied from 13.30 ± 0.684 to 26.03 ± 0.217 mg/g and tannin content varied from 8.72 ± 0.160 to 17.04 ± 0.206 mg/g. The free radical scavenging activity among the selected samples varied from 11.67 ± 0.221 to 38.29 ± 0.532 μg/ml. The correlation between the free radical scavenging activity with total phenol content (R 2 =0.511), with flavonoids (R 2 =0.241) and with tannin (R 2 =0.690) was calculated and maximum correlation value was found between tannin content and the free radical scavenging activity of the plant samples. The result supports that tannins were more responsible for free radical scavenging activity in the presently selected plants.
Beyond Data Mining : Integrative Machine Learning for Health Informatics

Machine learning (ML) aims at learning from data to gain knowledge from experience and to make predictions. ML is the most growing field in computer science and health is amongst the greatest application challenges. Health systems worldwide are confronted with big data, hence algorithms that improve through experience can be of great help and automatic ML (aML) approaches show impressive results. Though in health we are confronted with complex, heterogeneous, highdimensional, probabilistic, uncertain, incomplete, noisy data and sometimes with small data, or rare events, where aML-approaches suffer of insufficient training samples. Here interactive ML (iML) may be of help, which can be defined as ”algorithms that can interact with agents and can optimize their learning behaviour through these interactions, where the agents can also be human”. This human-in-the-loop can be beneficial in solving computationally hard problems, particularly with a doctor-inthe-loop, e.g. in subspace clustering, protein folding, or k-Anonymization, where human expertise can help to reduce an exponential search space through heuristic selection of samples. Therefore, what would otherwise be an NP-hard problem, reduces greatly in complexity through an human agent involved in the ML pipeline. However, for successful application of ML in health informatics it is of utmost importance to foster a concerted cross-disciplinary integrated effort of experts from 1) data science, 2) algorithms, 3) network science, 4) graphs/topology, 5) time/entropy, 6) data visualization, and 7) privacy, data protection, safety & security.
Distribution of the 9-bp mitochondrial DNA region V deletion among North American Indians.

The deletion of a 9-bp segment from the intergenic region between the mtDNA cytochrome oxidase II gene and the lysine tRNA gene has been documented mainly in individuals of East Asian ancestry and in individuals from East Asian-derived populations (e.g., Polynesia). Among Native Americans the deletion is absent among Eskimos and northern Na-Dene populations and present among most Amerind populations [sensu Greenberg (1987); i.e., all Native Americans except Eskimo-Aleut and Na-Dene] that have been studied. To better characterize the frequency and distribution of the 9-bp deletion in North America, we surveyed more than 400 individuals from 59 tribes representing a variety of linguistic groups. The absence of the deletion among Eskimo and northern Na-Dene populations is confirmed. Among Amerind groups the deletion is present in all groups represented by more than six individuals. The geographic distribution of the frequencies of the deletion appears to be clinal in North America. The deletion is absent in the Artic and Subartic and reaches its highest frequency in the Southwest. This distribution is consistent with the hypothesis that the ancestors of the Amerinds and Na-Dene arrived in the New World by means of separate migrations. The presence of the 9-bp deletion in high frequencies in all the major linguistic groups in the Southwest suggests that migration among tribes was common.
Effects of Group Counseling

Staff members of the Lucas County (Toledo, Ohio) Family Court Center became concerned because it seemed that while diagnosis of delinquent behavior was leaning toward an indi vidualized, differential technique, treatment methods were not correspondingly developed. The traditional one-to-one interview was often ineffective. In an effort to find useful alternative meth ods, several staff members who had participated in a group therapy experience themselves and had received in-service train ing in group methods decided to experiment. On the basis of prior social and psychological studies, six juvenile probationers were selected to participate in a series of group counseling sessions.
The Importance of Organizational Justice in Ensuring Research Integrity

The professional behavior of scientists, for good or ill, is likely associated with their perceptions of whether they are treated fairly in their work environments, including their academic department and university and by relevant regulatory bodies. These relationships may also be influenced by their own personal characteristics, such as being overcommitted to their work, and by the interactions between these factors. Theory also suggests that such associations may be mediated by negative or positive affect. We examined these issues using data from a national, mail-based survey administered in 2006 and 2007 to 5,000 randomly selected faculty from biomedical and social science departments at 50 top-tier research universities in the United States. We found that perceptions of justice in one's workplace (organizational justice) are positively associated with self-report of “ideal” behaviors and negatively associated with self-report of misbehavior and misconduct. By contrast, researchers who perceive that they are being unfairly treated are less likely to report engaging in “ideal” behaviors and more likely to report misbehavior and misconduct. Overcommitment to one's work is also associated with negative affect and interacts with perceptions of unfair treatment in ways that are associated with higher self-report of misbehavior. Thus, perceptions of fair treatment in the work environment appear to play important roles in fostering—or undermining—research integrity.
Papillomavirus-Like Particles Induce Acute Activation of Dendritic Cells1

The role of viral structural proteins in the initiation of adaptive immune responses is poorly understood. To address this issue, we focused on the effect of noninfectious papillomavirus-like particles (VLPs) on dendritic cell (DC) activation. We found that murine bone marrow-derived dendritic cells (BMDCs) effectively bound and rapidly internalized bovine papillomavirus VLPs. Exposure to fully assembled VLPs of bovine papillomavirus, human papillomavirus (HPV)16 or HPV18, but not to predominately disordered HPV16 capsomers, induced acute phenotypic maturation of BMDCs. Structurally similar polyomavirus VLPs bound to the DC surface and were internalized, but failed to induce maturation. DCs that had incorporated HPV16 VLPs produced proinflammatory cytokines IL-6 and TNF-α; however, the release of these cytokines was delayed relative to LPS activation. Production of IL-12p70 by VLP-exposed DCs required the addition of syngeneic T cells or rIFN-γ. Finally, BMDCs pulsed with HPV16 VLPs induced Th1-dominated primary T cell responses in vitro. Our data provide evidence that DCs respond to intact papillomavirus capsids and that they play a central role in VLP-induced immunity. These results offer a mechanistic explanation for the striking ability of papillomavirus VLP-based vaccines to induce potent T and B cell responses even in the absence of adjuvant.
Luminescence and Scintillation Characteristics of ${\rm Rb}_{2}{\rm LiCeBr}_{6}$  Single Crystal

Luminescence and scintillation properties of Rb2LiCeBr6 single crystal are presented. This is a new elpasolite scintillation material and is grown by vertical Bridgman technique. Under X-ray excitation, Ce3+ luminescence is observed between 365 and 415 nm. An energy resolution of 6.3% (FWHM) is obtained for the 662 keV full γ-ray absorption peak. The sample crystal shows good light yield of 33 000 ph/MeV of absorbed γ -ray energy. The sample crystal also shows 5.0% nonproportionality in the 31 to 1333 keV range. The luminescence decay time curve can be characterized by two decay time components with decay time constants of 55 ns (87%) and 284 ns (13%), respectively. Scintillation properties indicate that this compound can be an attractive candidate for medical imaging and radiation detection applications.
Integrative analysis of methylome and transcriptome in human blood identifies extensive sex- and immune cell-specific differentially methylated regions

The relationship between DNA methylation and gene expression is complex and elusive. To further elucidate these relations, we performed an integrative analysis of the methylome and transcriptome of 4 circulating immune cell subsets (B cells, monocytes, CD4+, and CD8+ T cells) from healthy females. Additionally, in light of the known sex bias in the prevalence of several immune-mediated diseases, the female datasets were compared with similar public available male data sets. Immune cell-specific differentially methylated regions (DMRs) were found to be highly similar between sexes, with an average correlation coefficient of 0.82; however, numerous sex-specific DMRs, shared by the cell subsets, were identified, mainly on autosomal chromosomes. This provides a list of highly interesting candidate genes to be studied in disorders with sexual dimorphism, such as autoimmune diseases. Immune cell-specific DMRs were mainly located in the gene body and intergenic region, distant from CpG islands but overlapping with enhancer elements, indicating that distal regulatory elements are important in immune cell specificity. In contrast, sex-specific DMRs were overrepresented in CpG islands, suggesting that the epigenetic regulatory mechanisms of sex and immune cell specificity may differ. Both positive and, more frequently, negative correlations between subset-specific expression and methylation were observed, and cell-specific DMRs of both interactions were associated with similar biological pathways, while sex-specific DMRs were linked to networks of early development or estrogen receptor and immune-related molecules. Our findings of immune cell- and sex-specific methylome and transcriptome profiles provide novel insight on their complex regulatory interactions and may particularly contribute to research of immune-mediated diseases.
A NOTE Multiple chromosomes in Burkholderia cepacia and B. gladioli and their distribution in clinical and environmental strains of B. cepacia

Burkholderia cepacia is found in soils and waters, it can be used in biocontrol and bioremediation but is also a human pathogen. It is not yet clear what differentiates pathogenic from non‐pathogenic strains of the organism. In this study the multiple replicon structure was investigated in 28 strains of B. cepacia by pulsed field gel electrophoresis. All strains examined, whether of clinical, environmental or plant pathogenic origin, were found to have two, three or four large (> 500 kbp) replicons. Many strains also contained small replicons. Clinical strains were more likely to have three or four large replicons than non‐clinical strains. Multiple replicon structure was also demonstrated in B. gladioli and Alcaligenes eutrophus.
The plasticity of extracellular fluid homeostasis in insects

ABSTRACT In chemistry, the ratio of all dissolved solutes to the solution's volume yields the osmotic concentration. The present Review uses this chemical perspective to examine how insects deal with challenges to extracellular fluid (ECF) volume, solute content and osmotic concentration (pressure). Solute/volume plots of the ECF (hemolymph) reveal that insects tolerate large changes in all three of these ECF variables. Challenges beyond those tolerances may be ‘corrected’ or ‘compensated’. While a correction simply reverses the challenge, compensation accommodates the challenge with changes in the other two variables. Most insects osmoregulate by keeping ECF volume and osmotic concentration within a wide range of tolerance. Other insects osmoconform, allowing the ECF osmotic concentration to match the ambient osmotic concentration. Aphids are unique in handling solute and volume loads largely outside the ECF, in the lumen of the gut. This strategy may be related to the apparent absence of Malpighian tubules in aphids. Other insects can suspend ECF homeostasis altogether in order to survive extreme temperatures. Thus, ECF homeostasis in insects is highly dynamic and plastic, which may partly explain why insects remain the most successful class of animals in terms of both species number and biomass. Summary: This Review examines the volume and osmotic concentration of the hemolymph in aquatic, terrestrial and gorging insects. Solute–volume plots illustrate the particular challenges to the hemolymph and the physiological responses.
Preprocessing Technique for Face Recognition Applications under Varying Illumination Conditions By

In the last years, face recognition has become a popular area of research in computer vision, it is typically used in network security systems and access control systems but it is also useful in other multimedia information processing areas. Performance of the face verification system depends on many conditions. One of the most problematic is varying illumination condition. In this paper, we discuss the preprocessing method to solve one of the common problems in face images, due to a real capture system i.e. lighting variations. The different stages include gamma correction, Difference of Gaussian (DOG) filtering and contrast equalization. Gamma correction enhances the local dynamic range of the image in dark or shadowed regions while compressing it in bright regions and is determined by the value of γ. DOG filtering is a grey scale image enhancement algorithm that eliminates the shadowing effects. Contrast equalization rescales the image intensities to standardize a robust measure of overall intensity variations. The technique has been applied to Yale-B data sets, Face Recognition Grand Challenge (FRGC) version 2 Experiment 4 and a real time created data set.
Instituting a radiology residency scholarly activity program

Background: The purpose of this manuscript is to present a newly instituted program for resident scholarly activity that includes a curriculum designed to enhance resident training with regard to research while meeting requirements established by the Accreditation Council for Graduate Medical Education (ACGME), the governing body responsible for regulation of post-graduate medical education and training in the United States. Methods: A scholarly activity program was designed with the following goals: (i) enhance the academic training environment for our residents; (ii) foster interests in research and academic career paths; (iii) provide basic education on research methodology and presentation skills. To guide program design, an electronic survey was created and distributed to the residents and faculty in the Department of Radiology and Radiological Sciences at the Medical University of South Carolina (MUSC), a 750-bed public teaching hospital in the state of South Carolina in the United States. Results: Survey respondents were in strong support of a required resident scholarly activity project (70% in favor), felt non-traditional projects were valuable (84.1% of respondents), and were proponents of required scholarly activity summary presentations (58%). This program requires that residents engage in a scholarly activity project under the guidance of a mentor. Resident success is maximized through in-house education initiatives focusing on presentation and research skills, protected time to work on the project, and oversight by a radiology research committee. All residents present a summary of their work near the end of their residency training. Discussion: Changes to the radiology resident certification process create an opportunity for incorporating new policies aimed at enhancing resident education. The scholarly activity program outlined in this manuscript is one such initiative designed to meet ACGME requirements, provide an introduction to research, and establish a scholarly activity project requirement.
TaqMan Systems for Genotyping of Disease-Related Polymorphisms Present in the Gene Encoding Apolipoprotein E

Abstract Polymorphisms of the gene encoding apolipoprotein E have been implicated in the pathogenesis of peripheral and coronary artery disease and neurodegenerative disorders such as sporadic and late-onset familial forms of Alzheimer's disease. We have developed TaqMan assay systems for the single nucleotide polymorphisms −219G/T, located in the promoter of the apolipoprotein E gene, 113G/C, present in the transcriptional enhancer element of intron 1, 334T/C, determining Cys or Arg as amino acid residue 112 of mature apolipoprotein E, and 472C/T, determining Arg or Cys as residue 158. The accuracy of genotype determination with the TaqMan systems was demonstrated by analyses with restriction endonucleases. We determined the genotypes of the apolipoprotein E polymorphisms in 2349 study subjects. The genotypes were distributed as: −219GG=27.3%, −219GT=49.1%, and −219TT=23.6% (p=0.435); 113GG=41.3%, 113GC=45.2%, and 113CC=13.5% (p=0.343); 334TT=73.4%, 334TC=24.7%, and 334CC=1.9% (p=0.539); 472CC=86.3%, 472CT=12.8%, and 472TT=0.9% (p=0.004) (Hardy-Weinberg equilibrium estimates are given in parentheses). The allele combinations which define the three major isoforms of apolipoprotein E, namely apoE2, apoE3, and apoE4, had the following allele frequencies: 334T/472T (∊2; 112Cys/158Cys)=7.3%, 334T/472C (∊3; 112Cys/158Arg)=78.4%, and 334C/472C (∊4; 112Arg/158Arg)=14.2%, respectively. ApoE genotypes were distributed as: ∊2∊2=0.9%, ∊2∊3=11.2%, ∊2∊4=1.6%, ∊3∊3=61.3%, ∊3∊4=23.1%, and ∊4∊4=1.9% (p=0.014). The TaqMan assays allow for fast and sensitive genotyping and are especially suitable for studies including large numbers of participants.
Halophilic diatom taxa are sensitive indicators of even short term changes in lowland lotic systems

Abstract The occurrence and spread of halophilic diatom taxa in freshwater lotic ecosystems are influenced both by natural processes and anthropogenic pollution. Diatom assemblages were regularly monitored in lowland lotic systems in Hungary (Central Europe) during the unusually dry year of 2012. Highly pronounced changes in diatom composition were observed from spring to autumn. Halophilic taxa (especially Nitzschia sensu lato species) appeared in the dry autumn. In addition, the total relative abundances of halophilic species also increased up to autumn. Abundance of Nitzschia cf. lorenziana and Nitzschia tryblionella showed a positive correlation with chloride and phosphate concentration, while that of other taxa like Tryblionella apiculata or Tryblionella calida showed a positive correlation with the concentration of nitrate. Our findings clearly demonstrated that these halophilic and mesohalophilic diatom taxa were sensitive indicators of even short-term changes in lowland lotic ecosystems, such as the increasing salt concentration from spring to autumn caused by the lack of rainfall and/or environmental loads.