Miracle-dz/UniFAR-FTUs huggingface dataset:
SCCRO3 (DCUN1D3) Antagonizes the Neddylation and Oncogenic Activity of SCCRO (DCUN1D1)*

Background: SCCRO3/DCUN1D3 is proposed to function as a tumor suppressor. However, the mechanisms are not well defined. Results: SCCRO3 inhibits SCCRO-promoted nuclear translocation and neddylation of cullins by sequestering them to the cell membrane. Conclusion: SCCRO3 putatively functions as a tumor suppressor by antagonizing the activity of SCCRO. Significance: Understanding how SCCRO and its family members cooperatively regulate cullin neddylation is important for neddylation-targeted cancer therapy. The activity of cullin-RING type ubiquitination E3 ligases is regulated by neddylation, a process analogous to ubiquitination that culminates in covalent attachment of the ubiquitin-like protein Nedd8 to cullins. As a component of the E3 for neddylation, SCCRO/DCUN1D1 plays a key regulatory role in neddylation and, consequently, cullin-RING ligase activity. The essential contribution of SCCRO to neddylation is to promote nuclear translocation of the cullin-ROC1 complex. The presence of a myristoyl sequence in SCCRO3, one of four SCCRO paralogues present in humans that localizes to the membrane, raises questions about its function in neddylation. We found that although SCCRO3 binds to CAND1, cullins, and ROC1, it does not efficiently bind to Ubc12, promote cullin neddylation, or conform to the reaction processivity paradigms, suggesting that SCCRO3 does not have E3 activity. Expression of SCCRO3 inhibits SCCRO-promoted neddylation by sequestering cullins to the membrane, thereby blocking its nuclear translocation. Moreover, SCCRO3 inhibits SCCRO transforming activity. The inhibitory effects of SCCRO3 on SCCRO-promoted neddylation and transformation require both an intact myristoyl sequence and PONY domain, confirming that membrane localization and binding to cullins are required for in vivo functions. Taken together, our findings suggest that SCCRO3 functions as a tumor suppressor by antagonizing the neddylation activity of SCCRO.
Source of the Variation in Meat and Bone Meal Nutritional Quality

The gross composition, gross amino acid content, apparent ileal amino acid digestibility and apparent ileal digestible amino acid content from 64 commercially produced meat and bone meals were statistically analysed. The samples were produced by 22 plants over a 2.5 year period with eight plants using batch dry rendering and 14 plants using low temperature rendering. A linear model with method and time of year (period) as fixed effects, plant within method as a random effect and sheep percent as a covariate was fitted to the composition data. The majority of the variation in the gross composition, amino acid digestibility and digestible amino acid content was explained by differences between plants using the same method. Neither rendering season nor origin of the raw materials contributed significantly to the observed variation in meat and bone meal protein quality. Rendering method (low temperature or batch rendering) had a significant effect on the variation observed in gross fat content, gross energy content, pepsin nitrogen digestibility, protein solubility and total lanthionine content. The digestibility of a number of amino acids and the apparent digestible content of arginine, cysteine, aspartic acid, proline and hydroxyproline were also significantly affected by rendering method. On average, batch dry and low temperature rendering systems produce meat and bone meals of similar nutritional quality. The variation between plant and within plant, however, is large, indicating that purchasing meat and bone meal from the same plant does not guarantee a consistent quality.
An Overview of the Pharmacokinetics and Pharmacodynamics of Efalizumab: A Monoclonal Antibody Approved for Use in Psoriasis

Efalizumab is a recombinant humanized monoclonal IgG1 antibody shown to be efficacious for the treatment of moderate to severe chronic plaque psoriasis. Efalizumab, a targeted inhibitor of T cell interactions, binds to the CD11a subunit of lymphocyte function—associated antigen 1 (LFA‐1), thereby preventing LFA‐1 binding to intercellular adhesion molecule 1 (ICAM‐1). The authors review the pharmacokinetic and pharmacodynamic data from the efalizumab clinical development program and discuss how these data led to selection of the optimal weekly subcutaneous (SC) dose of efalizumab (1.0 mg/kg) in adults. Efalizumab SC dosages of 1.0 mg/kg/wk or greater exerted maximal pharmacodynamic effects for CD11a expression and available CD11a binding sites on T lymphocytes. Dosages greater than 1.0 mg/kg/wk SC did not provide additional benefits; moreover, higher doses did not alter the safety profile. During long‐term administration of efalizumab, serum levels were generally stable and pharmacodynamic markers remained maximally affected.
Ab initio calculation of 4fN6s2 hyperfine structure in neutral rare-earth atoms.

The multiconfiguration Dirac-Fock (MCDF) method is used to calculate excitation energies, Lande g values, and hyperfine-structure (hfs) constants for the lowest multiplets of the 4f/sup N/6s/sup 2/ configurations of neutral rare-earth atoms. Although no adjustable parameters are used, the results are in rather good agreement with experiment. The calculated excitation energies and dipole hfs constants (using the known moment values) differ from experiment by typically 5%, and the g values by 0.1%. Relative to quadrupole moment values determined from muonic-atom hfs or Coulomb excitation, the calculated electric-quadrupole hfs is typically (30 +- 2)% too large, consistent with a Sternheimer shielding factor (not included in the MCDF calculations) of R/sub 4f/ = + 0.23. The calculated J dependence for all four observables is generally in good agreement with experiment for the ground multiplets. Particular cases where the MCDF results are less accurate are identified and discussed.
An efficient plane-grating monochromator based on conical diffraction for continuous tuning in the entire soft X-ray range including tender X-rays (2-8 keV).

Recently it was verified that the diffraction efficiency of reflection gratings with rectangular profile, when illuminated at grazing angles of incidence with the beam trajectory along the grooves and not perpendicular to them, remains very high for tender X-rays of several keV photon energy. This very efficient operation of a reflection grating in the extreme off-plane orientation, i.e. in conical diffraction, offers the possibility of designing a conical diffraction monochromator scheme that provides efficient continuous photon energy tuning over rather large tuning ranges. For example, the tuning could cover photon energies from below 1000 eV up to 8 keV. The expected transmission of the entire instrument is high as all components are always operated below the critical angle for total reflection. In the simplest version of the instrument a plane grating is preceded by a plane mirror rotating simultaneously with it. The photon energy selection will then be made using the combination of a focusing mirror and exit slit. As is common for grating monochromators for soft X-ray radiation, the minimum spectral bandwidth is source-size-limited, while the bandwidth can be adjusted freely to any larger value. As far as tender X-rays (2-8 keV) are concerned, the minimum bandwidth is at least one and up to two orders of magnitude larger than the bandwidth provided by Si(111) double-crystal monochromators in a collimated beam. Therefore the instrument will provide more flux, which can even be increased at the expense of a bandwidth increase. On the other hand, for softer X-rays with photon energies below 1 keV, competitive relative spectral resolving powers of the order of 10000 are possible.
Monitoring an Automated System for a Single Failure: Vigilance and Task Complexity Effects

The present study examined the effects of task complexity and time on task on the monitoring of a single automation failure during performance of a complex flight simulation task involving tracking, fuel management, and engine-status monitoring. Two groups of participants performed either all three flight simulation tasks simultaneously (multicomplex task) or the monitoring task alone (single-complex task); a third group performed a simple visual vigilance task (simple task). For the multicomplex task, monitoring for a single failure of automation control was poorer than when participants monitored engine malfunctions under manual control. Furthermore, more participants detected the automation failure in the first 10 min of a 30-min session than in the last 10 min of the session, for both the simple and the multicomplex task. Participants in the single-complex condition detected the automation failure equally well in both periods. The results support previous findings of inefficiency in monitoring automation and show that automation-related monitoring inefficiency occurs even when there is a single automation failure. Implications for theories of vigilance and automation design are discussed.
Sendai virus‐based expression of HIV‐1 gp120: reinforcement by the V(−) version

We have established a system for recovering Sendai virus (SeV), a nonsegmented negative strand RNA virus, entirely from cDNA at an extremely high rate, and have succeeded in creating a V(−) SeV whose gene expression was greatly enhanced by the deletion of the nonessential V gene. Because of its extreme medical importance, there has been a strong need for the establishment of a better system to express the gp120 envelope glycoprotein of the human immunodeficiency virus type 1 (HIV‐1) in sufficient quantity and purity. It also remains to be established to produce gp120 in in vitro natural host cells for HIV‐1 such as human primary blood mononuclear cells, macrophages or established T cell lines.
Pulmonary retention of ceramic fibers in silicon carbide (SiC) workers.

The fibrous inorganic content of post-mortem lung material obtained from 15 men who worked in the primary silicon carbide (SiC) industry was evaluated. Five men had neither lung fibrosis nor lung cancer (NFNC), six had lung fibrosis (LF), and four had lung fibrosis and lung cancer (LFLC). The workers had 23 to 32 years of exposure. Mean duration of exposure was 23.4 (SD 6.9) years in the NFNC group, 28.8 (SD 5.5) in the LF, and 32.3 (SD 9.0) in the LFLC group. Concentrations of SiC ceramic fibers and other fibrous minerals and angular particles were determined by transmission electron microscopy and energy dispersive spectroscopy. The geometric mean and geometric standard deviation lung concentrations of SiC ceramic fibers < 5 microns were not statistically different for the three groups (Mann-Whitney, p > 0.1). Pulmonary retention of SiC fibers > or = 5 microns showed an excess in LF and LFLC cases combined versus NFNC that approached statistical significance (Mann-Whitney, p = 0.06). There was a somewhat greater difference for lung retention of ferruginous bodies between NFNC and either LF or LFLC cases (Mann-Whitney, p = 0.02). SiC fibers > or = 5 microns and angular particles containing Si and especially ferruginous bodies were found at higher concentrations in LF and LFLC than in NFNC cases.
Salmonella fecal excretion control in broiler chickens by organic acids and essential oils blend feed added

Salmonellosis is an important disease with economic impact as it may affect animal performance and may result in foodborne disease in humans through the eggs and carcass contamination. Regarding the Salmonella control, it is possible to decrease its fecal excretion and the contamination of chicken carcasses by adding organic acids to the feed or drinking water at appropriate times. The aim of this study was to test a blend of organic acids and essential oils in broilers challenged with Salmonella Enteritidis (SE), and to verify the fecal excretion of Salmonella. Sixty broilers were placed in four groups. One group was the negative control. Another group was orally inoculated at 1 day-old with 10 5 CFU/mL of SE as a positive SE control. Two groups (T3 and T4) were orally inoculated at 1 day-old with 10 5 CFU/mL of SE and their feed was separately treated with 0.5 and 1% of organic acids and essential oils, respectively. To assess the fecal excretion of SE, cloacal swabs were collected from all birds at 2, 6, 13 and 20 days after inoculation. The T3 and T4 groups showed a reduction in fecal excretion of SE at 6 and 20 days after inoculation.
Heat-induced lethality and chromosomal damage in synchronized Chinese hamster cells treated with 5-bromodeoxyuridine.

SummarySynchronous Chinese hamster cells exposed to heat treatments of 45·5°c were very sensitive during S phase when chromosomal damage could account for cell killing, i.e. as for x-irradiated cells, the 37 per cent survival value for heated cells was observed when there was about one aberration per cell. However, the aberration frequency was very low and could not account for cell killing when the cells were heated, either during mitosis when they were most sensitive in terms of lethality, or during G1 when they were most resistant. Furthermore, about 50 per cent of the deletions in these cells heated in mitosis or G, occurred at the secondary constriction of the X chromosome. Incorporation of BUdR into DNA prior to synchronizing the cells had only an additive interaction with heat administered during G1 or S. This interaction was characterized by a reduction in the shoulder of the dose-response curves observed for both chromosomal aberrations and cell killing.These findings contrast greatly with those .
Prevalence of the metabolic syndrome in the mestizo population of peru.

BACKGROUND The metabolic syndrome (MS) has been shown to predict mortality due to cardiovascular disease. Currently, no population-based data on the prevalence of the MS is available in Peru. This study was aimed to assess the prevalence of the MS in urban Peruvian Mestizos, in the coastal districts of Lima, the capital of Peru. METHODS A cross-sectional, epidemiological survey was undertaken, including 612 unrelated subjects aged 30-92 years (68.3% females). Prevalence of the MS was defined by the National Cholesterol Education Program Expert Panel on Detection Evaluation, and Treatment of High Blood Cholesterol in Adults (ATPIII) criteria. Insulin resistance was estimated by the homeostasis model assessment (HOMA). RESULTS Age and sex standardized prevalence of the MS was 14.9% (13.2% in males, 16.5% in females). The MS was significantly more prevalent in females aged 45-59 years old (20.2% vs. 6.7%, p = 0.019). In individuals with the MS, the prevalence of insulin resistance (IR) was 45% in males/42% in females. Abdominal obesity (80% in males/92.8% in females), and low HDL cholesterol (55% males/75.4% females), but neither hypertriglyceridemia (85% in males/81.2% females) nor high fasting glucose (55% in males/36.2 % females) were more common in females. Prevalence of arterial hypertension was similar in both sexes. CONCLUSIONS In this Mestizo Peruvian population, prevalence of the MS is relatively low as compared to other ethnic groups; the higher prevalence in females is likely due to a higher prevalence of abdominal obesity. Overall, abdominal obesity and hypertriglyceridemia were the predominant combination of metabolic disorders in individuals fulfilling criteria for the diagnosis of the MS.
Functional impact of a spectrum of interstitial lung abnormalities in rheumatoid arthritis.

BACKGROUND Approximately 10% of patients with rheumatoid arthritis (RA) have interstitial lung disease (ILD), and one-third have subclinical ILD on chest CT scan. In this study, we aimed to further characterize functional decrements in a spectrum of RA-associated ILD. METHODS All subjects were enrolled in the Brigham and Women's Hospital Rheumatoid Arthritis Sequential Study (BRASS). The presence of interstitial lung abnormalities (ILAs) on clinically indicated chest CT scans was determined using a previously validated sequential reading method. Univariate and multivariate analyses were used to assess the association between degree of ILAs and physiologic, functional, and demographic variables of interest. RESULTS Of 1,145 BRASS subjects, 91 subjects (8%) were included in this study. Twelve had radiologically severe ILAs, 34 had ILAs, and 38 had no ILAs on CT scan. Subjects with radiologically severe ILAs were older (P = .0037), had increased respiratory symptoms (cough, P = .027; dyspnea, P = .010), and more severe RA disease (rheumatoid factor, P = .018; total swollen joints, P = .046) compared with subjects with no ILAs. Participants also had a trend toward having an increased smoking history (P = .16) and having lower FVC % predicted (77% vs 94%, P = .097) and diffusion capacity of carbon monoxide % predicted (52% vs 77%, P = .068). Similar but attenuated increases in respiratory symptoms, functional decrements, and RA disease severity were observed in subjects with ILAs compared with those with no ILAs. CONCLUSIONS We have shown that patients with RA have varying degrees of ILAs that are associated with a spectrum of functional and physiologic decrements. Our findings suggest that improved risk stratification and detection of ILAs will provide a therapeutic window that could improve RA-ILD outcomes.
Panic attacks: Klein's false suffocation alarm, Taylor and Rachman's data, and Ley's dyspneic-fear theory.

In a recent letter on Klein's 1 false suffocation alarm theory of panic attacks, Taylor and Rachman 2 reported two sets of data that purportedly support predictions derived from the theory. One set supports the validity of their measure of suffocation fear 3 as an index of the sensitivity of the putative suffocation alarm: "As predicted, subjects with high suffocation fear reported significantly more panic attacks than subjects with low suffocation fear. " 2 The other set gave the results of a brief structured interview, 2 in which subjects with high suffocation fears reported a significantly greater incidence of panic in enclosed spaces and in other situations and a greater incidence of spontaneous panic than did subjects with low suffocation fears. The primary point to be made here is that Taylor and Rachman's results also support Ley's 4 theory of panic, a theory predicated on the assumption that the classic "panic
Structure and Composition of Old-Growth and Unmanaged Second-Growth Riparian Forests at Redwood National Park, USA

Restoration of second-growth riparian stands has become an important issue for managers of redwood (Sequoia sempervirens (D. Don) Endl. ) forest reserves. Identifying differences between old-growth and second-growth forest vegetation is a necessary step in evaluating restoration needs and targets. The objective of this study was to characterize and contrast vegetation structure and composition in old-growth and unmanaged second-growth riparian forests in adjacent, geomorphologically similar watersheds at Redwood National Park. In the old-growth, redwood was the dominant overstory species in terms of stem density, basal area, and importance values. Second-growth was dominated by red alder (Alnus rubra Bong.), Douglas-fir (Pseudotsuga menziesii (Mirbel) Franco), and redwood. Understory species were similar in both forests, with several key differences: Oxalis oregana Nutt. and Trillium ovatum Pursh had greater importance values in the old-growth, and Vaccinium parvifolium Sm. , Dryopteris spp. and sedges Carex spp. had greater importance values in the second-growth. Notable differences in structure and composition suggest that restoration practices such as thinning could expedite the acquisition of old-growth characteristics in second-growth riparian forests.
Dynamic joint forces during knee isokinetic exercise

This study analyzed forces in the tibiofemoral and pa tellofemoral joints during isokinetic exercise using an analytical biomechanical model. The results show that isokinetic exercise can produce large loads on these joints, especially during extension exercises. The tibio femoral compressive force (4.0 body weight) is approx imately equal to that obtained during walking but it occurs at 55° of knee flexion. Anterior shear forces (resisting force to anterior drawer) exist during exten sion exercise at less than 40° of knee flexion, with a maximum of 0.3 body weight. Posterior shear forces (resisting force to posterior drawer) exist during exten sion exercise at knee joint angles greater than 40° and during the flexion portion of isokinetic exercise. The maximum posterior shear force is 1.7 body weight. The patellofemoral joint can encounter loads as high as 5.1 body weight which are 10 times higher than during straight leg raises. These results suggest that isokinetic exercise should be used cautiously in patients with knee lesions.
$H_\infty$-Filter Design for a Class of Networked Control Systems Via T–S Fuzzy-Model Approach

This paper is concerned with H ∞-design for a class of networked control systems (NCSs) with multiple state-delays via the Takagi-Sugeno (T-S) fuzzy model. The transfer delays and packet loss that are induced by the limited bandwidth of communication networks are considered. The focus of this paper is on the analysis and design of a full-order H ∞ filter, such that the filtering-error dynamics are stochastically stable, and a prescribed H ∞ attenuation level is guaranteed. Sufficient conditions are established for the existence of the desired filter in terms of linear-matrix inequalities (LMIs). An example is given to illustrate the effectiveness and applicability of the proposed design method.
Direct Sequence CDMA Power Control, Interleaving, and Coding

The authors develop and analyze models of power control that consider other aspects of code-division multiple access (CDMA) systems, such as interleaving and coding on the land mobile radio channel. The orientation is that a power control scheme keeps the received powers at the base station almost equal, and the performance degradation incurred if the powers are not exactly equal will be quantified. In doing so, the authors consider the performance implications of control latency and a maximum speech delay constraint. Because of positive correlations between the fading channel amplitudes, the effectiveness of the combination of interleaving and coding in combating the effects of power variations due to slow Rayleigh fading is reduced. It is shown that power control and interleaving/coding are most effective in complementary parameter regions, thus providing a degree of robustness for both fast and slow Rayleigh fading. >
High Sensitive and Selective Hydrogen Phosphate ISFET Based on Polyvinyl Chloride Membrane

The present work describes the preparation and characterization of polyvinyl chloride membrane doped with methyl-salin ionophore. This electrode was successfully used for selectivity and high sensibility of monohydroxiphosphate in aqueous solution. 1 μM detection limit of mono-hydrogen phosphate was obtained by using dibutyl phthalate and o-nitrophenyl octyl ether-poly vinyl chloride membrane in dynamic solution. The co-existence of a large excess of salts did not interfere with the detection in case of onitrophenyl octyl ether-poly vinyl chloride membrane that maked this selective electrode more adequate for the monohydrogen detection. Also, a highly selective and sensitive dihydrogen phosphate sensor was fabricated by autoassembly of polyvinyl chloride into silicium nitride gate of ISFET.
Association of tellurium resistance and bacteriophage inhibition conferred by R plasmids

Concomitant resistance to tellurium compounds (Ter) and inhibition of coli-phage development (Phi) are properties mediated by many H2 incompatibility group R plasmids which have been isolated from diverse bacterial and geographic sources. Ter plasmids from tellurium-resistant bacteria that were isolated from sewage and industrial wastes also mediated phage inhibition. Of these Ter plasmids, three from Citrobacter freundii belonged to the H incompatibility group, whereas three from Klebsiella pneumoniae did not.