Miracle-dz/UniFAR-FTUs huggingface dataset:
Brain renin-angiotensin system modifies the blood pressure response to intracerebroventricular cadmium in rats

In order to elucidate the involvement of the brain renin-angiotensin system (RAS) in cadmium intracerebroventricular (ICV) hypertension, we evaluated the effects of a pretreatment with different drugs: clonidine, an α2 adrenergic agonist, enalapril and captopril, both ACE inhibitors, and saralasin, a competitive nonselective AT1 and AT2 receptor antagonist. We used a rat strain with low levels of kallikrein (LKR) that was more sensitive to ICV cadmium hypertension, compared with normal kallikrein rats (NKRs), the control strain. The interplay between the kallikrein-kinin system and the RAS in the LKR strain caused various hemodynamic alterations, which we believe were the result of elevated RAS activity in these animals. Moreover, we suggest that the defective kallikrein-kinin system in LKR may also cause an alteration in the activation of brain RAS in these animals. The LKR displayed elevated concentrations of plasma AII, hypertrophy of the myocardium, and initial alterations in the renal glomerulotubular system. With the exception of clonidine, all of the other drugs showed greater antihypertensive effects of differing statistical significance in LKR, compared with NKR. Both ACE inhibitors were able to significantly reduce pressor response to cadmium ICV in LKR throughout the experiment, whereas in NKR, they were only able to reduce the hypertensive peak of cadmium. A significant protective effect was also observed in LKR pretreated with saralasin, while no effect was observed in NKR. These findings confirm the presence of brain RAS activation in LKR and its contribution to the central control of pressor response to cadmium ICV.
Impression of prognosis regarding pathologic stage after preoperative chemoradiotherapy in rectal cancer.

AIM To ascertain pathologic stage as a prognostic indicator for rectal cancer patients receiving preoperative chemoradiotherapy (PCRT). METHODS Patients with mid- and low rectal carcinoma (magnetic resonance imaging - based clinical stage II or III) between 2000 and 2009 and treated with curative radical resection were identified. Patients were divided into two groups: PCRT and No-PCRT. Recurrence-free survival (RFS) was examined according to pathologic stage and addition of adjuvant treatment. RESULTS Overall, 894 patients were identified. Of these, 500 patients received PCRT. Adjuvant chemotherapy was delivered to 81.5% of the No-PCRT and 94.8% of the PCRT patients. Adjuvant radiotherapy was given to 29.4% of the patients in the No PCRT group. The 5-year RFS for the No-PCRT group was 92.6% for Stage I, 83.3% for Stage II, and 72.9% for Stage III. The 5-year RFS for the PCRT group was 95.2% for yp Stage 0, 91.7% for yp Stage I, 73.9% for yp Stage II, and 50.7% for yp Stage III. CONCLUSION Pathologic stage can predict prognosis in PCRT patients. 5-year RFS is significantly lower among PCRT patients than No-PCRT patients in pathologic stage II and III. These results should be taken into account when considering adjuvant treatment for patients treated with PCRT.
[Effects of sirolimus on the growth of transplanted hepatocellular carcinoma].

OBJECTIVE To study the effects of sirolimus (SRL) on the growth of transplanted human hepatocellular carcinoma (HCC) in nude mice. METHODS HepG2 cells were Implanted into the liver of nude mice. The implanted mice were then treated with SRL and tacrolimus (FK506). The expression of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) was detected by immunohistology, microvessel density (MVD) was counted by immunostaining with anti-CD34 antibody for endothelial cells. Tumor apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. RESULTS The tumor weight was (352+/-38) mg, (683+/-53) mg and (675+/-45) mg in SRL, FK506 and control group respectively. The tumor weight was significantly decreased in SRL group (P < 0.01), and there was no difference between FK506 group and control group. The expression of VEGF and PCNA protein was remarkably down-regulated in SRL group compared to control group (P < 0.05), and it was not significantly different between FK506 group and control group (P > 0.05). Compared to the control group, MVD was significanly decreased in SRL group, and the apoptosis index of tumor cell was significantly higher in SRL group (P < 0.01). CONCLUSION SRL inhibits transplanted HCC tumor growth by reducing tumor angiogenesis, inhibiting tumor proliferation and inducing tumor apoptosis.
The Royal Touch

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Occurrence and Regional Distribution of Apoptosis in Scoliotic Discs

Study Design. Seventy surgically obtained intervertebral discs from 9 patients with idiopathic and 7 patients with neuromuscular scoliosis were analyzed for the regional distribution of apoptosis. Objectives. To investigate the role of apoptotic mechanisms in scoliotic discs. Summary of Background Data. The reasons for the development of scoliosis are complex and yet not completely understood. In herniated lumbar disc tissue, increased apoptosis and a high expression of Fas and Fas ligand and caspase-3 activity have already been reported, suggesting a pivotal role of apoptotic mechanisms in intervertebral disc degeneration. In scoliotic discs, cell death was correlated with disc deformity and changes in nutrient supply and metabolic levels. The role of apoptosis in scoliotic discs, however, is still unclear. Methods. Apoptosis was determined by terminal deoxyribonucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay and poly(ADP-ribose) polymerase (PARP) p85 immunohistochemistry. Expression of Fas and Fas-ligand was analyzed by immunohistochemistry and reverse transcription polymerase chain reaction analysis. Results. In all samples analyzed, apoptotic cells could be detected in the nucleus, anulus, and endplate. The number of apoptotic cells was significantly higher in the nucleus compared to the other areas and in the apex versus the nonapex discs. There was no difference between the discs of idiopathic and neuromuscular scoliosis and between the 2 age groups studied (10–17 years and 17–48 years, respectively). A strong expression of Fas and Fas-ligand could be detected in all samples. Conclusion. Increased numbers of apoptotic cells in the nucleus of scoliotic discs and the apex disc suppose a pivotal role of programmed cell death for the progression of this common disorder. The simultaneous increase of Fas and Fas-ligand expression in areas with increased cell death point to an activation of the apoptotic process via the Fas/Fas-L system.
Phenotypic and Proteomic Analysis of the Aspergillus fumigatus ΔPrtT, ΔXprG and ΔXprG/ΔPrtT Protease-Deficient Mutants

Aspergillus fumigatus is the most common mold species to cause disease in immunocompromised patients. Infection usually begins when its spores (conidia) are inhaled into the airways, where they germinate, forming hyphae that penetrate and destroy the lungs and disseminate to other organs, leading to high mortality. The ability of hyphae to penetrate the pulmonary epithelium is a key step in the infectious process. A. fumigatus produces extracellular proteases that are thought to enhance penetration by degrading host structural barriers. This study explores the role of the A. fumigatus transcription factor XprG in controlling secreted proteolytic activity and fungal virulence. We deleted xprG, alone and in combination with prtT, a transcription factor previously shown to regulate extracellular proteolysis. xprG deletion resulted in abnormal conidiogenesis and formation of lighter colored, more fragile conidia and a moderate reduction in the ability of culture filtrates (CFs) to degrade substrate proteins. Deletion of both xprG and prtT resulted in an additive reduction, generating a mutant strain producing CF with almost no ability to degrade substrate proteins. Detailed proteomic analysis identified numerous secreted proteases regulated by XprG and PrtT, alone and in combination. Interestingly, proteomics also identified reduced levels of secreted cell wall modifying enzymes (glucanases, chitinases) and allergens following deletion of these genes, suggesting they target additional cellular processes. Surprisingly, despite the major alteration in the secretome of the xprG/prtT null mutant, including two to fivefold reductions in the level of 24 proteases, 18 glucanases, 6 chitinases, and 19 allergens, it retained wild-type virulence in murine systemic and pulmonary models of infection. This study highlights the extreme adaptability of A. fumigatus during infection based on extensive gene redundancy.
Spontaneous ambulatory activity as a quantifiable outcome measure for rheumatoid arthritis.

OBJECTIVE To validate the objective monitoring of ambulatory activity as an outcome measure for rheumatoid arthritis (RA). METHODS We have compared ambulatory activity to a range of currently favoured outcome measures, ranging from subjective opinions to X-ray damage, in a population of 93 RA sufferers. RESULTS Correlations were stronger with measures of joint damage and disability, and less strong with measures of disease activity. Sensitivity to change was good. Three different interventions were compared for the quantity of the response, and the results agree with clinical experience, with steroid injection of the knee and use of non-steroidal anti-inflammatory drugs (NSAIDs) having a similar response and the provision of surgical shoes producing a more modest increase in ambulation. CONCLUSION The measurement of ambulatory activity has validity for RA assessment. It provides different but related data to the currently used measures. It is objective, relevant, quantifiable and of unlimited scale. It could be used to quantify interventions aimed at increasing ambulation, in carefully constructed studies.
Regression techniques for the prediction of stock price trend

This paper examines the theory and practice of regression techniques for prediction of stock price trend by using a transformed data set in ordinal data format. The original pretransformed data source contains data of heterogeneous data types used for handling of currency values and financial ratios. The data formats in currency values and financial ratios provide a process for computation of stock prices. The transformed data set contains only a standardized ordinal data type which provides a process to measure rankings of stock price trends. The outcomes of both processes are examined and appraised. The primary design is based on regression analysis from WEKA machine learning software. The stock price movement in Bursa Malaysia is used as our research setting. The data sources are corporate annual reports which included balance sheet, income statement and cash flow statement. The variables included in the data set were formed based on stock market trading fundamental analysis approach. Classifiers in WEKA were used as algorithms to produce the outcomes. This study showed that the outcomes of regression techniques can be improved for the prediction of stock price trend by using a dataset in standardized ordinal data format.
Hospitalization patterns for psychiatric disorders across the lifespan in Australia from July 1998 to June 2005.

OBJECTIVE This study examined specialized psychiatric hospitalization in Australia. METHODS Data from July 1998 to June 2005 from the National Hospital Morbidity Database, which includes all public and most private hospital stays in Australia, were analyzed. RESULTS Over the study period, there were 1,343,584 psychiatric discharges (including transfers, deaths, and changes in care type). There was an 18.0% increase in discharges per 1,000 persons and a 20.3% reduction in average length of stay. The rate of discharges decreased with age, and length of stay increased with age. Psychiatric admission rates were higher and length of stay was lower for women than for men. Hospitalization rates fell with age for schizophrenia, manic disorders, and substance use disorders; increased with age for organic disorders; and peaked midlife for alcohol abuse and mood disorders. A late-life increase in hospitalization for depressive and personality disorders was observed for men. CONCLUSIONS Planning for specialized psychiatric hospital services needs to take into account the mix of clinical needs by age, gender, and diagnosis.
Contrast-enhanced MR of the facial nerve in patients with posttraumatic peripheral facial nerve palsy.

PURPOSE To estimate the value of noncontrast and contrast-enhanced T1-weighted MR imaging in detecting the underlying mechanisms of injury and regeneration in immediate- or delayed-onset posttraumatic peripheral facial nerve palsy. METHODS Twenty-four patients with posttraumatic peripheral facial nerve palsy were examined on a 1.5-T MR imaging unit with precontrast and postcontrast T1-weighted spin-echo and gradient-echo sequences. RESULTS Abnormal enhancement of the distal intrameatal nerve segment was visible in 92% of the patients up to 2 years after their initial trauma. A hematoma within the geniculate ganglion was seen in 33% of the patients with a longitudinal fracture. The greater superficial petrosal nerve (in 32% of patients) and the geniculate ganglion (in 48% of patients) were thick and intensely enhancing. Hematoma within the cochlea/vestibule or enhancement of the cochlea/vestibule and the vestibulocochlear (eighth) nerve was observed in transverse fractures. CONCLUSION MR images can show long-lasting abnormal nerve enhancement, especially in the distal intrameatal nerve segment, related to the long-lasting breakdown of the blood/peripheral nerve barrier associated with nerve degeneration and regeneration after traumatic stretching of the greater superficial petrosal nerve. Additionally, intraoperatively observed perineural and intraneural scar formation leads to thickening and intense enhancement of the affected nerve segments on MR images. A hematoma in the region of the geniculate ganglion can be seen in some but not all patients. Associated damage of the inner ear structures in patients with transverse fractures is also visible on MR images.
Thermodynamics of a beta-hairpin structure: evidence for cooperative formation of folding nucleus.

To elucidate early nucleation stages in protein folding, multi-probed thermodynamic characterization was applied to the beta-hairpin structural formation of G-peptide, which is a C-terminal fragment of the B1 domain of streptococcal protein G. The segment corresponding to the sequence of G-peptide is believed to act as a nucleus during the folding process of the B1 domain. In spite of the broad thermal transition of G-peptide, nuclear magnetic resonance (NMR) melting measurements combined with our original analytical theory enabled us to obtain the thermodynamic properties of the beta-hairpin formation with considerable accuracy. Additionally, all the thermodynamic properties determined by every NMR probe on both the main-chain and the side-chains were quite similar, and also comparable to the values that were independently determined by calorimetric analysis of G-peptide. These results demonstrate that G-peptide folds cooperatively throughout the molecule. In other words, the formation of the beta-hairpin is interpreted as the fashion of a first-order phase transition between two states without any distinguishable intermediates. This cooperative formation of the short linear peptide consisting of only 16 residues provides insight into not only the first folding events of the B1 domain, but also the general principles of proteins in terms of structural hierarchy, stability and folding mechanism.
Transepidermal Water Loss During Halogen Spotlight Phototherapy in Preterm Infants

Among preterm infants there is a relationship between skin blood flow and transepidermal water loss (TEWL). The aim of this study was to assess whether halogen spotlight phototherapy without significant heat stress increases TEWL and affects maintenance fluid requirements in preterm infants. TEWL was measured noninvasively before the start and after 1 h of halogen spotlight phototherapy in a group of preterm infants, nursed in double-walled incubators with moderately high relative humidity. Relative humidity and ambient temperature in the incubator were tightly controlled. Mean ± SD birth weight of the 18 infants was 1412 ± 256 g, gestational age 30.6 ± 1.6 wk, and age at measurement 5 ± 3 d. Nine infants received ventilatory assistance. Relative humidity was 40–80% (mean 52%). Average TEWL increased from 13.6 to 16.5 g/m2/h during phototherapy. These data show that TEWL increases by approximately 20% during phototherapy despite constant skin temperature and relative humidity. Maintenance fluids of preterm infants should be increased by 0.35 mL/kg/h during exposure to halogen spotlight phototherapy.
The value of molecular analysis by PCR in the diagnosis of cutaneous lymphocytic infiltrates

The diagnosis and classification of cutaneous lymphomas remain a challenge for the clinician and dermatopathologist. This diagnostic dilemma is mainly encountered in the distinction between an early malignant lymphoma and a benign reactive lymphocytic infiltrate (pseudolymphoma). Until the beginning of the 1980s, our diagnostic tools were limited to the clinical presentation, course, and histopathology in diagnosis and classification of lymphocytic infiltrates. Advances in immunology and, in particular, in molecular genetics with the introduction of the Southern blot technique and the polymerase chain reaction (PCR) have revolutionized the diagnosis of lymphocytic infiltrates by determination of clonality. In some series, more than 90% of cutaneous T‐cell lymphomas have a clonal rearrangement of the T‐cell receptor γ‐chain gene, as opposed to very low percentages of rearrangement in T‐cell pseudolymphomas.
Daily transpiration rates of woody species on drying soil.

Among annual plants, daily transpiration rates, expressed as a fraction of volumetric soil water content available for transpiration, show a common pattern in response to soil drying. Initially, as soil dries, there is little decrease in transpiration rate until water availability has fallen to about one third that at field capacity. With further soil drying, relative transpiration rate decreases in a more-or-less linear fashion until all available water has been used. Data previously obtained for perennial woody species have often been confounded by different methods for determining available soil water. In this study, we investigated the daily transpiration response to soil drying in five woody perennial species: Thuja plicata Donn ex D. Don, Acer rubrum L. , Robinia pseudoacacia L. , Hibiscus sp. and Ibex aquifolium L. Transpiration was unaffected by soil drying until the initial estimated transpirable soil water fraction had decreased to between 0.23 and 0.32 of that at field capacity. Beyond this point, transpiration rate declined linearly with available soil water fraction until reaching one fifth the rate observed in well-watered plants. With further soil drying, the relative transpiration rates remained between 10 and 20% of that observed in well-watered plants. Maintenance of transpiration at these rates with further soil drying was hypothesized to result from contributions to transpiration of water stored in plant tissues. After taking tissue water storage into account, it was estimated that transpiration was curtailed as the available soil water fraction fell to between 0.26 and 0.37 of that at field capacity, which is comparable to values reported for annual crop plants.
Cellular electrophysiological effects of flecainide on human atrial fibres.

STUDY OBJECTIVE The aim of the study was to examine the electrophysiological characteristics of human atrial specimens collected during heart surgery and to investigate the effects of the class I antiarrhythmic agent flecainide on their electrical activity. DESIGN Atrial specimens were studied using standard microelectrode techniques, with and without superfused flecainide (5 x 10(-7) M) or the transient outward current inhibitor 4-aminopyridine (0.5 mM). EXPERIMENTAL MATERIAL Atrial fragments 0.5-1.0 cm2 were obtained at operation from 34 patients, mean age 30 years. There was no history of previous atrial arrhythmia in any patient and drug therapy was stopped 24 h before surgery. MEASUREMENTS AND MAIN RESULTS Two types of transmembrane action potential were identified: (1) triangular shaped potentials (group A, classically found in animal models); (2) potentials with a large plateau preceded by a notch (group B). The effect of flecainide was compared on the the two types of action potential. In both, flecainide lessened the depolarisation rate. In group B, but not in group A, it increased the action potential duration at 50% and 90% repolarisation (APD50, APD90) and the effective refractory period. The notch in group B action potentials is generated by transient outward currents (Ito). Inhibition of these currents, either by increasing the pacing rate or by adding 4-aminopyridine, limited the increase in APD50, APD90, and effective refractory period generated by the presence of flecainide. CONCLUSIONS The effects of flecainide on the atrial repolarisation process depend on the shape of the action potential. These effects are more marked in cells with a plateau, where Ito is activated.
Effects of a pharmacist's medication review in nursing homes

Background Older people in nursing and residential homes often have complex disabilities and behavioural disturbances. Recent publicity has highlighted the dangers of medication in this group, and controls over prescribing have been suggested. Aims To investigate the effect of a review of medication by a pharmacist. Method An 8-month prospective trial of an active medication review by a pharmacist was carried out on 330 residents in nursing homes in Manchester. Results The intervention group experienced greater deterioration in cognitive function and behavioural disturbance than the control group, but the changes in depression and quality of life were similar for both groups. The number of drugs prescribed fell in the intervention group, but not in the control group, with a corresponding saving in drug costs. The number of deaths was significantly smaller in the intervention homes during the intervention period (4 v. 14) but not overall during the study period as a whole (26 v. 28). Conclusion This clinical intervention reduced the number of medicines prescribed to elderly people in nursing homes, with minimal impact on their morbidity and mortality.