Miracle-dz/UniFAR-FTUs huggingface dataset:
Elevated plasma CaM expression in patients with acute cerebral infarction predicts poor outcomes and is inversely associated with miR-26b expression

Background: Calcium overload plays an important role in ischemia/reperfusion injury during ischemic brain damage and is mediated by calmodulin (CaM). However, the understanding of the regulatory mechanisms of CaM expression at the gene level is limited. The expression levels of miR-26b change significantly during ACI, and bioinformatic analyses predict that miR-26b would be a potential regulator of calmodulin (CALM1) mRNA. This study aimed to determine the expression of miR-26b and CaM in the plasma of patients with ACI and investigate the impact of miR-26b on CALM1 expression. Methods: CaM and miR-26b expression analyses from the plasma of patients with ACI and normal controls were performed using ELISA and qRT-PCR, respectively. Correlations between CaM, miR-26b, and NIHSS scores were analyzed. Then, miR-26b mimics and inhibitors were transfected into HUVE cell lines via lipofectamine. CALM1 mRNA expression in HUVECs was detected by RT-PCR, and the protein levels were detected by Western blot. Results: Plasma CaM expression in patients with ACI was significantly higher when compared with normal controls, and miR-26b expression was significantly lower. The plasma levels of CaM and miR-26b were correlated with the NIHSS scores in ACI patients. miR-26b modulated CALM1 in vitro. The transfected miR-26b mimic and inhibitor significantly altered the expression of CALM1/CAM at the mRNA and protein levels in cultured HUVECs. Conclusions: CaM might be a potential novel blood marker in patients with ACI. miR-26b targeted CALM1 and affected the expression of CaM at the post-transcriptional level, which likely contributed to the progression of ACI brain injury.
At home in the oasis: Middle-class newcomers’ affiliation to their deprived Rotterdam neighbourhood

One of the arguments for ‘social mix’ urban renewal in low-income neighbourhoods is that the presence of middle-class residents would improve life chances for lower-income groups. However, according to various researchers, middle-class newcomers have little social interaction with the neighbourhood, do not feel at home there and make little use of the neighbourhood’s public spaces and facilities. In short, they show disaffiliation with their mixed neighbourhoods, thus compromising the assumed positive effects of social mixing. Several studies, on the other hand, point to different factors that mediate this (dis)affiliation, such as newcomers’ lifestyles, housing trajectories, the width of class and ethnic differences between newcomers and the existing population and the presence of neighbourhood shops and facilities that can cater to both groups. This relatively large set of factors suggests a need for detailed case-study research to understand neighbourhood affiliation of middle-class newcomers. We made a qualitative and quantitative study of a housing complex designed specifically for middle-class buyers with a ‘diversity-liking lifestyle’, in a poor neighbourhood in Rotterdam, the Netherlands. It has not been researched before how using the assumed preferences of diversity-liking middle-class households in dwelling design, for social mix, relates to the eventual residents’ neighbourhood affiliation. The study yields hardly any disaffiliation in the sense of exclusionary spatial strategies: almost all residents use (semi-)public spaces in the neighbourhood on a day-to-day basis. Affiliation in terms of self-identification with the neighbourhood, however, is higher for residents with a diversity-liking lifestyle, and only the minority-ethnic residents use neighbourhood primary schools.
Innovation Pathways Towards Creating Integrated Value: A Conceptual Framework

In the context of a plethora of worsening social, environmental and ethical negative conditions often associated with economic growth and industrial activity, I am proposing Integrated Value as a conceptual and practical framework for business to respond credibly and effectively as a force for innovation and solutions. In order to do so, I begin by asking: how is value to society currently being destroyed by economic activities? Taking a systems science perspective, the answer is: whenever it causes fragmentation, or disintegration. My contention is that this disintegration in society occurs in at least five principle ways, which I call the five forces of fragmentation: disruption, disconnection, disparity, destruction and discontent. I then ask: how might this this value destruction in society be countered or reversed? And I find clues in innovations that are occurring in five emerging economic spheres: the resilience, Exponential, access, circular and wellbeing economies. In each of these areas, there are breakthrough business models, practices, products and services that are building, rather than destroying, societal value. I call these the five pathways to innovation, defined in terms of the desired future state they are trying to advance, which is a society that is more secure, smart, shared, sustainable and satisfying. Four strategic value-creation options are then described (singular, focused, diffuse and integrated value) before citing illustrative cases and describing the seven steps of a methodology to implement integrated value.
Adult- and childhood-onset systemic lupus erythematosus: a comparison of onset, clinical features, serology, and outcome.

This study examines the differences which may distinguish systemic lupus erythematosus (SLE) presenting in adult life or childhood. A common database was established, with analysis of clinical, serological and outcome features of a cohort of patients with SLE, with disease diagnosed before the age of 16 (n = 39) or after the age of 16 (n = 165). Disease onset was generally more severe in the childhood-onset patients. Cardiopulmonary disease was more common in the older-onset group, but major haematological manifestations were more frequent in the childhood-onset group. Serologically, anti-DNA, anti-Sm and anti-RNP antibodies and a low C3 were all found more frequently in the younger patients. Twice as many adult-onset cases had died at the time of the last follow-up (10 vs 5%), but this group had been followed for a longer period (average 7.5 yr, S.D. 3.9 for adults vs average 4.8 yr, S.D. 3.2 for children). However, the younger patients were twice as likely (82 vs 40%) to require high-dose prednisone, although the requirement for immunosuppressive agents was similar in the two groups. Clinicians should anticipate that children with SLE have a more severe disease onset than adults in general.
PAR1‐mediated RhoA activation facilitates CCL2‐induced chemotaxis in PC‐3 cells

Patients with advanced prostate cancer often exhibit increased activation of the coagulation system. The key activator of the coagulation cascade is the serine protease thrombin which is capable of eliciting numerous cellular responses. We previously reported that the thrombin receptor PAR1 is overexpressed in prostate cancer. To investigate further the role of PAR1 in prostate cancer metastasis, we examined the effects of thrombin activation on cell adhesion and motility in PC‐3 prostate cancer cells. Activation of PAR1‐induced dynamic cytoskeletal reorganization and reduced PC‐3 binding to collagen I, collagen IV, and laminin (P < 0.01) but not fibronectin. Expression of the cell surface integrin receptors did not change as assessed by flow cytometry. Immunofluorescence microscopy revealed that PAR1 stimulation caused reorganization of the focal adhesions, suggesting that PAR1 activation in PC‐3 cells may be modulating cell adhesion through integrin function but not expression. Furthermore, RhoA was activated upon stimulation with thrombin with subsequent cell contraction, decreased cell adhesion, and induced migration towards monocyte chemoattractant protein 1 (MCP‐1; CCL2). Thus, it appears that thrombin stimulation plays a role in prostate cancer metastasis by decreasing cell adhesion to the extracellular matrix and positioning the cell in a “ready state” for migration in response to a chemotactic signal. Further exploration is needed to determine whether PAR1 activation affects other signaling pathways involved in prostate cancer. J. Cell. Biochem. 101:1292–1300, 2007. © 2007 Wiley‐Liss, Inc.
The Rise of Uncontested Elections in Indonesia: Case Studies of Pati and Jayapura

Abstract: This article explains a new trend in Indonesian local politics: the rise of uncontested elections. We explore this trend by way of detailed examinations of two such elections in February 2017: the district head election in Pati, Central Java; and the mayoral election in Jayapura, Papua. In doing so, we consider explanations that have been advanced elsewhere, including those that focus on “scare-off effects” and incumbency advantages. Though such approaches are relevant, we show that there was a contrast between our two cases: in Pati, the strength of the incumbent and his wealthy running mate dissuaded rival candidates and parties from competing; in Jayapura, two other candidates wanted to run, and even secured backing from local party branches, but their candidacies were annulled after legal challenges. If the first pathway showed a process of broad-based elite bargaining producing a “win-win solution”, in Jayapura the pathway involved a zero-sum-game contest between rival elites. Despite these differences, in both cases there was competition between local elites, but it happened prior to the election. Overall, we argue the rise of uncontested elections points to growing elite entrenchment in local politics.
The transient depression of hippocampal CA1 LTP induced by chronic intermittent ethanol exposure is associated with an inhibition of the MAP kinase pathway

Using electrophysiological and biochemical approaches, we investigated the effects of chronic, intermittent ethanol (CIE) treatment on activation of the mitogen activated protein kinase (MAPK), also known as extracellular signal regulated protein kinase 1 and 2. In hippocampal slices taken from control rats, brief high‐frequency stimulation to Schaffer collateral fibers induced a large post‐tetanic potentiation (PTP) in the CA1 region that decayed to stable long‐term potentiation (LTP) of field extracellular postsynaptic potentials. Western blot analyses showed that phosphorylation of MAPK was increased during PTP and returned to baseline levels during LTP. In slices from the rats removed immediately from CIE treatment, PTP and MAPK activation during the PTP was significantly less than that observed in control slices and LTP was absent. In slices from rats subjected to 1 day withdrawal from CIE treatment, both the reduction in MAPK phosphorylation during PTP and the impairment of PTP and LTP were still evident. Recovery of PTP and partial recovery of LTP was observed in slices obtained from 5‐day withdrawn rats. However, MAPK activation during PTP was still attenuated significantly. Interestingly, MAPK activation was enhanced significantly during LTP in 5‐day withdrawn rats as well as the sensitivity to MAPK inhibitor PD 098059. In addition to these changes in HFS‐induced MAPK activation, we also observed a significant reduction in the basal phosphorylation of MAPK in slices removed from rats immediately after CIE treatment. These results implicate the MAPK signal transduction pathway as a potential cellular target of ethanol. Alterations in MAPKs could play an important role in the alcohol‐induced changes in synaptic plasticity associated with the effects of alcohol abuse on learning and memory processes.
Dietary balance during pregnancy is associated with fetal adiposity and fat distribution.

BACKGROUND The prenatal environment can induce permanent changes in offspring phenotype. Thinness at birth is associated with adult risk of cardiometabolic disease. OBJECTIVE The objective was to investigate the association between maternal nutrition during pregnancy and intrauterine development of fetal body composition. DESIGN We used prospective data from 179 Australian women with singleton pregnancies from the Women and Their Children's Health Study. A validated food-frequency questionnaire was used at 18-24 wk and 36-40 wk of gestation to quantify maternal diet during the previous 3 mo of pregnancy. Fetal body-composition measurements were ascertained from abdominal and midthigh sites by ultrasound performed at 19, 25, 30, and 36 wk. The subcutaneous fat area at each site was calculated by subtracting the lean/visceral area from the total area. RESULTS In linear mixed-model regressions, maternal intakes of protein (b = -0.13; P = 0.04) and starch (b = 0.10; P = 0.02) and the protein:carbohydrate ratio (b = -3.61; P = 0.02) were associated with the percentage of abdominal fat, whereas SFA (b = 0.27; P = 0.04) and PUFA (b = -0.48; P = 0.03) were associated with the percentage of midthigh fat. Response surfaces for fetal adiposity were maximized at different macronutrient intakes. Abdominal fat was highest with low protein intakes (<16% of energy), and midthigh fat was highest at intermediate protein (18-21% of energy), high fat (>40% of energy), and low carbohydrate (<40% of energy) intakes. CONCLUSION Fetal body composition may be modifiable via nutritional intervention in the mother and thus may play an important role in influencing the offspring's risk of future disease.
Quantifying long-term changes in carbon stocks and forest structure from Amazon forest degradation

Despite sustained declines in Amazon deforestation, forest degradation from logging and fire continues to threaten carbon stocks, habitat, and biodiversity in frontier forests along the Amazon arc of deforestation. Limited data on the magnitude of carbon losses and rates of carbon recovery following forest degradation have hindered carbon accounting efforts and contributed to incomplete national reporting to reduce emissions from deforestation and forest degradation (REDD+). We combined annual time series of Landsat imagery and high-density airborne lidar data to characterize the variability, magnitude, and persistence of Amazon forest degradation impacts on aboveground carbon density (ACD) and canopy structure. On average, degraded forests contained 45.1% of the carbon stocks in intact forests, and differences persisted even after 15 years of regrowth. In comparison to logging, understory fires resulted in the largest and longest-lasting differences in ACD. Heterogeneity in burned forest structure varied by fire severity and frequency. Forests with a history of one, two, and three or more fires retained only 54.4%, 25.2%, and 7.6% of intact ACD, respectively, when measured after a year of regrowth. Unlike the additive impact of successive fires, selective logging before burning did not explain additional variability in modeled ACD loss and recovery of burned forests. Airborne lidar also provides quantitative measures of habitat structure that can aid the estimation of co-benefits of avoided degradation. Notably, forest carbon stocks recovered faster than attributes of canopy structure that are critical for biodiversity in tropical forests, including the abundance of tall trees. We provide the first comprehensive look-up table of emissions factors for specific degradation pathways at standard reporting intervals in the Amazon. Estimated carbon loss and recovery trajectories provide an important foundation for assessing the long-term contributions from forest degradation to regional carbon cycling and advance our understanding of the current state of frontier forests.
The relationship between exposure duration and sulfur dioxide-induced bronchoconstriction in asthmatic subjects.

The purpose of this study was to determine the shortest duration of exposure to 1.0 ppm sulfur dioxide (SO2) sufficient to induce bronchoconstriction significantly greater than that observed with exposure to clean air (CA) in exercising SO2-sensitive asthmatics. Asymptomatic, nonmedicated, male asthmatics (n = 12) with airway hyperresponsiveness to both methacholine and SO2 were exposed in a chamber (20 degrees C, 40% relative humidity) for 0.0, 0.5, 1.0, 2.0 and 5.0 min to both CA and 1.0 ppm SO2 on separate days (10 exposures). Just prior to each exposure, subjects walked on a treadmill in CA for 5 min at a predetermined speed/elevation to elicit a target ventilation of about 40 L/min, i.e., a brisk pace up a slight incline. After this walk, subjects rapidly entered an adjoining exposure chamber containing either CA or SO2 and immediately walked at the same speed/elevation for the specified exposure duration. Subjects then rapidly exited the chamber. Specific airway resistance (SRaw) and ratings of respiratory symptoms associated with asthma [shortness of breath/chest discomfort (SB/CD) and wheezing (WHZ)] were measured prior to any exercise and following each exposure. Postexposure SRaw and symptom ratings increased with increased exposure duration in SO2; postexposure SRaw also was increased with increased exposure duration in CA but to a lesser extent. After adjusting for the CA response, significantly greater SO2-induced bronchoconstriction was observed for the 2.0 and 5.0 min exposures as indicated by substantially greater increases in SRaw and substantially higher ratings of respiratory symptoms. The authors conclude that with the above exposure conditions, on average, SO2-sensitive asthmatics exhibit significant bronchoconstriction at exposure durations of 2.0 min or more.
Temsirolimus, interferon alfa, or both for advanced renal-cell carcinoma.

BACKGROUND Interferon alfa is widely used for metastatic renal-cell carcinoma but has limited efficacy and tolerability. Temsirolimus, a specific inhibitor of the mammalian target of rapamycin kinase, may benefit patients with this disease. METHODS In this multicenter, phase 3 trial, we randomly assigned 626 patients with previously untreated, poor-prognosis metastatic renal-cell carcinoma to receive 25 mg of intravenous temsirolimus weekly, 3 million U of interferon alfa (with an increase to 18 million U) subcutaneously three times weekly, or combination therapy with 15 mg of temsirolimus weekly plus 6 million U of interferon alfa three times weekly. The primary end point was overall survival in comparisons of the temsirolimus group and the combination-therapy group with the interferon group. RESULTS Patients who received temsirolimus alone had longer overall survival (hazard ratio for death, 0.73; 95% confidence interval [CI], 0.58 to 0.92; P=0.008) and progression-free survival (P<0.001) than did patients who received interferon alone. Overall survival in the combination-therapy group did not differ significantly from that in the interferon group (hazard ratio, 0.96; 95% CI, 0.76 to 1.20; P=0.70). Median overall survival times in the interferon group, the temsirolimus group, and the combination-therapy group were 7.3, 10.9, and 8.4 months, respectively. Rash, peripheral edema, hyperglycemia, and hyperlipidemia were more common in the temsirolimus group, whereas asthenia was more common in the interferon group. There were fewer patients with serious adverse events in the temsirolimus group than in the interferon group (P=0.02). CONCLUSIONS As compared with interferon alfa, temsirolimus improved overall survival among patients with metastatic renal-cell carcinoma and a poor prognosis. The addition of temsirolimus to interferon did not improve survival. (ClinicalTrials.gov number, NCT00065468 [ClinicalTrials.gov].).
Extremely Low Exposure of a Community to Severe Acute Respiratory Syndrome Coronavirus: False Seropositivity due to Use of Bacterially Derived Antigens

ABSTRACT Estimates of seropositivity to a new infectious agent in a community are useful to public health. For severe acute respiratory syndrome (SARS), the figures are conflicting. Herein, we screened 12,000 people in a community stricken by SARS 10 months previously and found 53 individuals (0.44%) who had immunoglobulin G antibodies to the SARS coronavirus (SARS-CoV) nucleocapsid (N) produced in bacteria. However, only seven of these (group 1) had sera which also reacted with the native N antigen expressed in SARS-CoV-infected Vero cells, N-transfected 293T cells, and tissues of infected SARS patients. Of these, six individuals had had SARS previously. The remaining person, as well as the 46 other individuals (group 2), were healthy and had no history of SARS. Group 1 antibodies recognized epitopes located slightly differently in N from those of group 2 antibodies, and a mouse hybridoma antibody resembling the former type was generated. Unusually, group 2 antibodies appeared to recognize cross-reactive bacterial epitopes that presumably were posttranslationally modified in eukaryotes and hence were probably not induced by SARS-CoV or related coronaviruses but rather by bacteria. The N antigen is thus highly unique. The extremely low rate (0.008%) of asymptomatic SARS infection found attests to the high virulence of the SARS-CoV virus.
Highly Skilled or Highly Wanted Migrants? Conceptualizations, Policy Designs and Implementations of High-skilled Migration Policies

This special issue offers an opportunity to delve into the construction of migrant categories through policy design and policy implementation. It proposes to widen the focus beyond immigration authorities in order to include actors that are in one or another way involved in the process of selecting, supporting or employing highly skilled workers and therefore also contributing to their definition. The aim of the special issue is to bring to the surface the indistinct objectives of immigration policies, and to analyse the interplay between policies, discourses and practices. More precisely, we discuss the argument that the definition of highly skilled migrants depends more on how potential migrants are viewed by interest groups than on migrants’ characteristics.
KECANTIKAN WANITA KOREA SEBAGAI KONSEP KECANTIKAN IDEAL DALAM IKLAN NEW POND’S WHITE BEAUTY: WHAT OUR BRAND AMBASSADORS ARE SAYING

This study aims to reveal the discourse contained in the advertisement of New POND'S White Beauty: What Our Brand Ambassadors are Saying and its relation to the life of the people in Southeast Asia. The researcher uses the critical discourse analysis method by Norman Fairclough to examine the advertisement which portrays the brand ambassadors from Indonesia, the Philippines and Vietnam's assesments about POND'S White Beauty product which is claimed to make their skin as white as Korean women. The researcher focused on the text analysis and social praxis based on the content of the advertisement. The researcher also adopted Poscolonial approach, focusing on the use of mimicry theory by Homi K. Bhabha after knowing the link between the text in the advertisement and the social praxis. By using mimicry theory, the researcher is able to know the perception of women in Southeast Asia, especially Indonesia, about Korean women who have smooth and white skin. The researcher finds that the New POND'S White Beauty: What Our Brand Ambassadors are Saying advertisement shows the discourse of Korean women's beauty skin as the new ideal standard of beauty which believed by Southeast Asian. It is represented by the three brand ambassadors in the advertisement. Thus, it can be said that South Korea as one country in Asia has the power to spread its culture and colonize other countries there. It also shows that colonization of Asian countries is no longer only done by Western countries, but also by the fellow Asian countries themselves.