Miracle-dz/UniFAR-FTUs huggingface dataset:
Establishing the community structure of signed interconnected graph in data

Graph communities can be an efficient and robust way to identify the clustering structure of data. A common scenario is the case that data matrix can be represented by graphs from instance and attribute. How to associate the consistent community structure in the two data matrices from instance and attribute respectively are important for understanding the data. We propose a novel and simple method to construct a interconnected network, by combining the instance and attribute graphs of the data as the interconnections between them, which unlike the bipartite graph cluster or the bicluster approaches. The community structure can be detected with an extended Q-modularity algorithm that also takes negative weighted edges into consideration, where each community can contain both instances and attributes that provide the association. Toy models are provided to illustrate the efficiency of the method. Real data of waste water treatment plants are treated by this method and the consistent community structure associated with day/measurements can be found.
SUN1 Interacts with Nuclear Lamin A and Cytoplasmic Nesprins To Provide a Physical Connection between the Nuclear Lamina and the Cytoskeleton

ABSTRACT Nuclear migration and positioning within cells are critical for many developmental processes and are governed by the cytoskeletal network. Although mechanisms of nuclear-cytoskeletal attachment are unclear, growing evidence links a novel family of nuclear envelope (NE) proteins that share a conserved C-terminal SUN (Sad1/UNC-84 homology) domain. Analysis of Caenorhabditis elegans mutants has implicated UNC-84 in actin-mediated nuclear positioning by regulating NE anchoring of a giant actin-binding protein, ANC-1. Here, we report the identification of SUN1 as a lamin A-binding protein in a yeast two-hybrid screen. We demonstrate that SUN1 is an integral membrane protein located at the inner nuclear membrane. While the N-terminal domain of SUN1 is responsible for detergent-resistant association with the nuclear lamina and lamin A binding, lamin A/C expression is not required for SUN1 NE localization. Furthermore, SUN1 does not interact with type B lamins, suggesting that NE localization is ensured by binding to an additional nuclear component(s), most likely chromatin. Importantly, we find that the luminal C-terminal domain of SUN1 interacts with the mammalian ANC-1 homologs nesprins 1 and 2 via their conserved KASH domain. Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position.
Treatment of advanced stages of hallux rigidus with cheilectomy and phalangeal osteotomy.

BACKGROUND Surgical treatment of hallux rigidus has usually consisted of cheilectomy for mild to moderate disease and arthrodesis for more advanced disease. The reported failure rate for cheilectomy alone in patients with advanced disease is approximately 37.5%. We reported our results with the combination of cheilectomy and extension osteotomy at the proximal phalanx for the treatment of advanced hallux rigidus. METHODS Between 2000 and 2007, eighty-one patients with advanced hallux rigidus (classified as Hattrup and Johnson Grade III) underwent a unilateral cheilectomy and great toe proximal phalangeal extension osteotomy. Outcome assessment was determined by comparison of preoperative and postoperative American Orthopaedic Foot & Ankle Society scores, radiographs, first metatarsophalangeal joint motion, and patient satisfaction. Sixty-four of the eighty-one patients had complete clinical and radiographic examinations at a minimum duration of follow-up of two years. RESULTS The mean duration of follow-up was 4.3 years. The mean dorsiflexion of the first metatarsophalangeal joint improved significantly (p < 0.05), by 27.0°, from 32.7° preoperatively to 59.7° postoperatively. The average American Orthopaedic Foot & Ankle Society scores improved significantly (p < 0.05) from 67.2 points preoperatively to 88.7 points postoperatively. Radiographs of the interphalangeal joint made postoperatively showed no evidence of development of interphalangeal joint arthritis. Of the eighty-one patients, sixty-nine (85.2%) were satisfied with the results of treatment and four (4.9%) subsequently underwent arthrodesis to treat persistent symptoms at the first metatarsophalangeal joint. CONCLUSIONS To our knowledge, this study is the first to support the use of a combination of cheilectomy and extension osteotomy of the great toe proximal phalanx as an alternative to first metatarsophalangeal joint arthrodesis to manage patients with advanced hallux rigidus.
Business Models and Sustainability in Nature Tourism: A Systematic Review of the Literature

This study explores the business model literature within nature tourism, focuses on its sustainability-related aspects, and adopts some of the results of the literature review by Reinhold et al. The research questions concern how scholars use and operationalize the business model concept in the context of nature tourism, and to what extent sustainability-related aspects are included and discussed. A literature review was conducted including a total of 18 scientific articles from various disciplines. The findings suggest that scientific literature about business models in nature tourism is very limited, both in relation to the number of articles and their content. With regard to the latter, the business model concept is sometimes adopted without any clear definition, sustainability-related aspects—especially those relative to the environmental dimension—are scarcely discussed, the perspective adopted is usually static, and innovation for sustainability is only marginally included. This indicates a clear gap in the literature and a considerable potential for future studies.
Characteristics and origin of myogenic response in isolated gracilis muscle arterioles.

Responses to changes in intravascular pressure of isolated rat gracilis muscle arterioles were investigated under no-flow conditions. First-, second-, and third- order arterioles were isolated and cannulated. Vascular diameters were measured with an image-shearing device and then recorded. In response to the step increases in perfusion pressure (from 20 to 160 mmHg, by 10- or 20-mmHg steps) arterioles constricted and developed active tone. For example, at 100, 80, and 50 mmHg pressure the steady-state active diameters of 1st-, 2nd-, and 3rd-order arterioles were 76.9 +/- 1.6, 32.3 +/- 1.1 and 22.3 +/- 3.2 microns, respectively. At the same perfusion pressure, by use of a Ca(2+)-free solution (ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid; 1 mM) containing sodium nitroprusside (SNP; 10(-4) M), the passive diameters (PD) of these vessels were 161.8 +/- 3.2, 76.0 +/- 1.7, and 47.6 +/- 2.2 microns. The negative slopes of the pressure-diameter curves indicate that in the physiological pressure range an inverse relationship exists between the arteriolar diameter and intravascular pressure. The maximum constriction expressed as a percent of PD was similar in the various sized arterioles (approximately 60%) but was reached at lower pressures in the smaller vessels. The vasoactive function of endothelium and vascular smooth muscle was assessed by the responses of arterioles to acetylcholine (ACh; 10(-6) M) and SNP (5 x 10(-8) M) before and after removal of the endothelium with air. After removal of the endothelium, dilation to ACh was abolished while dilation to SNP was retained. (ABSTRACT TRUNCATED AT 250 WORDS)
Global uncertainty and capital flows: any difference between foreign direct investment and portfolio investment?

ABSTRACT This note investigates how global uncertainty relates to extreme waves of capital flows, including foreign direct investment, portfolio investment, and other investment. We find the clear differences in the role of global uncertainty between advanced and developing economies. Global uncertainty increases the likelihood of sudden contraction of portfolio investment in both advanced and developing economies, while it increases that of foreign direct investment in only advanced economies.
Food , drug , insect sting allergy , and anaphylaxis Household peanut consumption as a risk factor for the development of peanut allergy

Background: Most children with peanut allergy (PA) react on first known oral exposure to peanut. Recent data suggest cutaneous exposure as a route of sensitization. Objectives: This study aimed to establish the relevant route of peanut exposure in the development of allergy. Methods: Questionnaires were administered to children with PA and to high-risk controls (with egg allergy) and controls without allergy. Questionnaires were completed before subjects were aware of their PA status, avoiding recall bias. Questionnaires recorded maternal peanut consumption during pregnancy, breast-feeding, and the first year of life. Peanut consumption was determined among all household members, allowing quantification of environmental household exposure (household peanut). Results: Median weekly household peanut in the 133 PA cases was significantly elevated (18.8 g) compared with 150 controls without allergy (6.9 g) and 160 high-risk controls (1.9 g). There were no differences in infant peanut consumption between groups. Differences in maternal peanut consumption during pregnancy (and lactation) were significant but become nonsignificant after adjusting for household peanut. A doseresponse relationship was observed between environmental (nonoral) peanut exposure and the development of PA, which was strongest for peanut butter. Early oral exposure to peanut
Identification of a repeated sequence element required for efficient encapsidation of the adenovirus type 5 chromosome

Adenovirus type 5 deletion mutants that lack portions of their cis-acting DNA encapsidation signal synthesized nearly normal levels of viral DNA and late polypeptides but failed to efficiently package the DNA into virus particles. A series of mutant viruses carrying small deletions were produced and used to identify a repeated element (AGTAAATTTGGGC and AGTAAGATTTGGCC) as a key component of the packaging signal. One copy of the repeat was sufficient to signal efficient packaging. The packaging domain could function near either end of the viral chromosome but was no longer active when moved several hundred base pairs toward the interior of the DNA molecule.
Safe Credit to the Poor: The Role of Anti‐Usury Policies

Usury regulations focus both on explicit recommendations, such as disclosure statements in lending acts or interest rate caps, and on incentives for the formal banking sector to reach the poor. Considerable attention is also devoted to adequate sanctions for the practice of usury. We propose a theoretical model to investigate where to direct reforms to curb usury. Our primary policy implication is the convenience of polarizing the allocation of public resources in either legal safeguards for formal lenders or sanction enforcement against usurers. Furthermore, we demonstrate that in developing countries such policy interventions may backfire when borrowers' higher wealth implies increasing inequality in its distribution. Therefore, countries undertaking reforms against predatory lending should be aware of their growth path during transition.
Systematic identification of microRNA functions by combining target prediction and expression profiling

Target predictions and validations are major obstacles facing microRNA (miRNA) researchers. Animal miRNA target prediction is challenging because of limited miRNA sequence complementarity to the targets. In addition, only a small number of predicted targets have been experimentally validated and the miRNA mechanism is poorly understood. Here we present a novel algorithm for animal miRNA target prediction. The algorithm combines relevant parameters for miRNA target recognition and heuristically assigns different weights to these parameters according to their relative importance. A score calculation scheme is introduced to reflect the strength of each parameter. We also performed microarray time course experiments to identify downregulated genes due to miRNA overexpression. The computational target prediction is combined with the miRNA transfection experiment to systematically identify the gene targets of human miR-124. miR-124 overexpression led to a significant downregulation of many cell cycle related genes. This may be the result of direct suppression of a few cell growth inhibitors at the early stage of miRNA overexpression, and these targeted genes were continuously suppressed over a long period of time. Our high-throughput approach can be generalized to globally identify the targets and functions of other miRNAs.
The First Mammalian Aldehyde Oxidase Crystal Structure

Background: Aldehyde oxidases have pharmacological relevance, and AOX3 is the major drug-metabolizing enzyme in rodents. Results: The crystal structure of mouse AOX3 with kinetics and molecular docking studies provides insights into its enzymatic characteristics. Conclusion: Differences in substrate and inhibitor specificities can be rationalized by comparing the AOX3 and xanthine oxidase structures. Significance: The first aldehyde oxidase structure represents a major advance for drug design and mechanistic studies. Aldehyde oxidases (AOXs) are homodimeric proteins belonging to the xanthine oxidase family of molybdenum-containing enzymes. Each 150-kDa monomer contains a FAD redox cofactor, two spectroscopically distinct [2Fe-2S] clusters, and a molybdenum cofactor located within the protein active site. AOXs are characterized by broad range substrate specificity, oxidizing different aldehydes and aromatic N-heterocycles. Despite increasing recognition of its role in the metabolism of drugs and xenobiotics, the physiological function of the protein is still largely unknown. We have crystallized and solved the crystal structure of mouse liver aldehyde oxidase 3 to 2.9 Å. This is the first mammalian AOX whose structure has been solved. The structure provides important insights into the protein active center and further evidence on the catalytic differences characterizing AOX and xanthine oxidoreductase. The mouse liver aldehyde oxidase 3 three-dimensional structure combined with kinetic, mutagenesis data, molecular docking, and molecular dynamics studies make a decisive contribution to understand the molecular basis of its rather broad substrate specificity.
Energy Consumption and GGP in the OECD Countries: A Causality Analysis

Abstract This study investigated the nature or direction of causality between GDP, electricity consumption and total energy consumption in the OECD. Secondary data was used while both the ordinary least square (OLS) and generalized method of moments (GMM) estimators were employed to test for causality in our model. Our result found the presence of a bi-directional causality between energy consumption and GDP for the total energy demand model and between electricity consumption and GDP for the electricity demand model. By implication, the bi-directional causality in our estimated models suggest that both energy consumption and GDP are important factors in economic development in the OECD. Thus, if misguided policy measures are made to reduce energy consumption it could have a detrimental effect on GDP which will slow down economic growth. A recommendation is for policy makers to concentrate on encouraging energy efficiency as a way to reduce energy and electricity consumption.
Identification of Flow-dependent Endothelial Nitric-oxide Synthase Phosphorylation Sites by Mass Spectrometry and Regulation of Phosphorylation and Nitric Oxide Production by the Phosphatidylinositol 3-Kinase Inhibitor LY294002*

Endothelial cells release nitric oxide (NO) acutely in response to increased laminar fluid shear stress, and the increase is correlated with enhanced phosphorylation of endothelial nitric-oxide synthase (eNOS). Phosphoamino acid analysis of eNOS from bovine aortic endothelial cells labeled with [32P]orthophosphate demonstrated that only phosphoserine was present in eNOS under both static and flow conditions. Fluid shear stress induced phosphate incorporation into two specific eNOS tryptic peptides as early as 30 s after initiation of flow. The flow-induced tryptic phosphopeptides were enriched, separated by capillary electrophoresis with intermittent voltage drops, also known as “peak parking,” and analyzed by collision-induced dissociation in a tandem mass spectrometer. Two phosphopeptide sequences determined by tandem mass spectrometry, TQpSFSLQER and KLQTRPpSPGPPPAEQLLSQAR, were confirmed as the two flow-dependent phosphopeptides by co-migration with synthetic phosphopeptides. Because the sequence (RIR)TQpSFSLQER contains a consensus substrate site for protein kinase B (PKB or Akt), we demonstrated that LY294002, an inhibitor of the upstream activator of PKB, phosphatidylinositol 3-kinase, inhibited flow-induced eNOS phosphorylation by 97% and NO production by 68%. Finally, PKB phosphorylated eNOS in vitro at the same site phosphorylated in the cell and increased eNOS enzymatic activity by 15–20-fold.
Conditional Ablation of MHC-II Suggests an Indirect Role for MHC-II in Regulatory CD4 T Cell Maintenance1

Although the importance of MHC class II (MHC-II) in acute homeostatic proliferation of regulatory T (Treg) cells has been established, we considered here the maintenance and state of Treg cells in mice that are almost completely devoid of MHC-II in their periphery but still make their own CD4 T cells and Treg cells. The latter was accomplished by conditional deletion of a loxP-flanked MHC-II β-chain allele using a TIE2Cre transgene, which causes a very high degree of deletion in hemopoietic/endothelial progenitor cells but without deletion among thymic epithelial cells. Such conditional MHC-II-deficient mice possess their own relatively stable levels of CD4+CD25+ cells, with a normal fraction of Foxp3+ Treg cells therein, but at a level ∼2-fold lower than in control mice. Thus, both Foxp3low/− CD4+CD25+ cells, said to be a major source of IL-2, and IL-2-dependent Foxp3+ Treg cells are reduced in number. Furthermore, CD25 expression is marginally reduced among Foxp3+ Treg cells in conditional MHC-II-deficient mice, indicative of a lack of MHC-II-dependent TCR stimulation and/or IL-2 availability, and IL-2 administration in vivo caused greatly increased cell division among adoptively transferred Treg cells. This is not to say that IL-2 can cause Treg cell division in the complete absence of MHC-II as small numbers of MHC-II-bearing cells do remain in conditional MHC-II-deficient mice. Rather, this suggests only that IL-2 was limiting. Thus, our findings lend support to the proposal that Treg cell homeostasis depends on a delicate balance with a population of self-reactive IL-2-producing CD4+CD25+ cells which are themselves at least in part MHC-II-dependent.
Activity—Rest Rhythm Abnormalities in Parkinson's Disease Patients are Related to Dopaminergic Therapy

ABSTRACT Objectives: To compare going to bed, waking-up, and immobility onset and offset times in Parkinson's disease (PD) patients receiving levodopa and/or dopamine agonists (i.e., dopaminergic therapy or DT) with “de novo” patients and healthy controls and to correlate these parameters with self-reported sleep disturbances. Methods: Fifteen “de novo” and 60 PD patients on DT were included in the study. Twenty-three subjects without history of sleep complaints and similar age and sex distributions as the PD patients served as controls. Going to bed and waking-up times were registered in 7-day sleep logs. Nocturnal immobility onset and offset times were measured using actigraphy. Sleep disorders were assessed applying the PD sleep scale (PDSS). Results: Patients on DT showed earlier waking-up and immobility offset times (40 min) compared with controls and “de novo” patients. Multivariable regression analysis showed that DT but not Hoehn & Yahr score was a significant and independent predictor of early awakening time (OR == 4.0, 95%% CI == 1.15–14.13). Patients manifesting more frequent nighttime psychotic symptoms and more frequent daytime somnolence showed earlier going to bed and waking-up times. Conclusions: Results suggest that activity–rest rhythm alteration in PD may be a consequence of DT but not of disease stage. Altered sleep cycles correlated with diurnal somnolence and nocturnal psychosis.
The search for the most reliable long-term rain attenuation CDF of a slant path and the impact on prediction models

Many prediction methods of long term complementary distribution function (CDF) of rain attenuation based on rain intensity CDF, when tested against concurrent beacon measurements show very similar large errors. In our opinion, these tests may indicate that the real concurrency of rain attenuation and rain intensity CDFs is not sure. The scope of the paper is to show that this can happen even in a well designed and controlled experiment. To this end, we have used the measurements collected at Spino d'Adda of Italsat 18.7-GHz beacon in a 37.7/spl deg/ slant path, and the independent predictions of the synthetic storm technique (SST) for the same radio link, to search the most reliable long term rain attenuation CDF of the slant path, that we found given by the SST CDF. Since the search is based on concurrent and independent rain intensity measurements, we think that our conclusion is sound. We have also discussed how to complete, statistically, a rain attenuation data bank with the SST prediction (such as the large and valuable Italsat data bank) to provide a secure experimental set of concurrent rain attenuation and rain intensity statistics to test prediction methods under controlled conditions, like in a laboratory.
Scalable and Sustainable: How to Build Anticipatory Capacity into Social Protection Systems

Climate shocks contribute to a significant share of the humanitarian burden, and are a key factor in increasing poverty and food insecurity. Social protection is increasingly recognised as an instrument to help build resilience to climate risks through long-term, large-scale national systems. However, most experiences to date have focused on social protection’s role for chronic needs, or at best, shock-response, rather than on anticipation and prevention. This article argues that social protection can support more effective resilience building at scale by integrating early action and preparedness. We propose a concrete solution, namely linking a Forecast-based Financing mechanism to a social protection system to enable anticipatory actions based on forecast triggers and guaranteed funding ahead of a shock. Such a system may enhance scalability, timeliness, predictability and adequacy of social protection benefits. Key considerations for success of this emerging approach include sound analysis of forecast, risks, cost and benefits, and ring-fenced funding.