Miracle-dz/UniFAR-FTUs huggingface dataset:
Differential expression of epidermal growth factor receptor in juvenile and adult‐onset recurrent respiratory papillomatosis

1. Karaman E, Mercan H, Ozdilek A et al. Huge arteriovenous malformation in masseter muscle. J. Craniofac. Surg. 2009; 20; 1292–1294. 2. Vargel I, Cil BE, Er N et al. Hereditary intraosseous vascular malformation of the craniofacial region: an apparently novel disorder. Am. J. Med. Genet. 2002; 109; 22–35. 3. Werner JA, Eivazi B, Folz BJ et al. State of the art of classification, diagnostics and therapy for cervicofacial hemangiomas and vascular malformations. Laryngorhinootologie 2006; 85; 883–891. 4. Pusztaszeri MP, Seelentag W, Bosman FT. Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues. J. Histochem. Cytochem. 2006; 54; 385–395. 5. Jackson DG. The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends Cardiovasc. Med. 2003; 13; 1–7. 6. Banerji S, Ni J, Wang SX et al. LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan. J. Cell Biol. 1999; 144; 789–801. 7. Xu H, Edwards JR, Espinosa O et al. Expression of a lymphatic endothelial cell marker in benign and malignant vascular tumors. Hum. Pathol. 2004; 35; 857–861. 8. Mulliken JB, Glowacki J. Hemangiomas and vascular malformations in infants and children: a classification based on endothelial characteristics. Plast. Reconstr. Surg. 1982; 69; 412–422. 9. Lee BB, Laredo J, Lee TS et al. Terminology and classification of congenital vascular malformations. Phlebology 2007; 22; 249– 252.
Prolyl Hydroxylase Domain (PHD) 2 Affects Cell Migration and F-actin Formation via RhoA/Rho-associated Kinase-dependent Cofilin Phosphorylation*

Cells are responding to hypoxia via prolyl-4-hydroxylase domain (PHD) enzymes, which are responsible for oxygen-dependent hydroxylation of the hypoxia-inducible factor (HIF)-1α subunit. To gain further insight into PHD function, we generated knockdown cell models for the PHD2 isoform, which is the main isoform regulating HIF-1α hydroxylation and thus stability in normoxia. Induction of a PHD2 knockdown in tetracycline-inducible HeLa PHD2 knockdown cells resulted in increased F-actin formation as detected by phalloidin staining. A similar effect could be observed in the stably transfected PHD2 knockdown cell clones 1B6 and 3B7. F-actin is at least in part responsible for shaping cell morphology as well as regulating cell migration. Cell migration was impaired significantly as a consequence of PHD2 knockdown in a scratch assay. Mechanistically, PHD2 knockdown resulted in activation of the RhoA (Ras homolog gene family member A)/Rho-associated kinase pathway with subsequent phosphorylation of cofilin. Because cofilin phosphorylation impairs its actin-severing function, this may explain the F-actin phenotype, thereby providing a functional link between PHD2-dependent signaling and cell motility.
The effect of exercise interventions on inflammatory biomarkers in healthy, physically inactive subjects: a systematic review

Background Increases in physical activity ameliorate low-grade systemic inflammation in disease populations such as type 2 diabetes mellitus and coronary artery disease. The effects of aerobic and resistance training (RT) on inflammatory biomarker profiles in non-disease, physically inactive individuals are unknown. Methods A systematic review of randomized controlled trials measuring the effect of aerobic and resistance exercise on pro-inflammatory biomarkers in healthy, inactive adult populations was conducted. The available peer-reviewed literature was searched from January 1990 to June 2016 using the electronic databases PubMed and Scopus. A narrative synthesis of review findings was constructed with discussion of the impact of aerobic, resistance and combined training on C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-8, interleukin-1β and tumour necrosis factor-α. Results The initial search revealed 1596 potentially relevant studies. Application of the study eligibility criteria led to the full-text review of 54 articles with 11 studies deemed suitable for inclusion. Review of related articles and the reference lists of the 54 full-text articles led to the inclusion of 2 additional studies. The review revealed inconsistent findings relating to the effect of aerobic training and RT on CRP and IL-6. Studies of older-aged adults (>65 years old) demonstrated the greatest and most consistent reduction in inflammatory biomarkers post-training intervention. Conclusions A paucity of evidence exists relating to the effect of exercise training on inflammatory markers in non-disease, physically inactive adults. The available evidence suggests potential for the greatest benefit to be seen in older populations and with higher intensity aerobic exercise.
Factores de riesgo para infecciones urinarias bajas en embarazadas mayo 1999-marzo 2000

Los cambios anatomofisiologicos propios de la gestacion condicionan el aumento de la frecuencia de las infecciones urinarias bajas. Existen multiples factores de riesgo para adquirir estas infecciones ademas de la condicion de gravidez. Entre estos se encuentran edad de la paciente, numero de gestas, vida sexual activa y edad gestacional. Es por ello que en este estudio se investiga la relacion entre el embarazo y dichos factores. Se diseno un estudio descriptivo, de casos y controles. El universo lo constituyo 206 embarazadas que asistieron al control prenatal del Hospital Universitario de Caracas entre mayo de 1999 y marzo de 2000. Para ello se utilizaron modelos de encuesta de grupos seleccionados y revision de las historias clinicas. Dicho estudio revelo un mayor porcentaje de infecciones urinarias durante el embarazo en el grupo etario de 20 y 25 anos (31 por ciento), en el segundo trimestre de embarazo (41,8 por ciento), en las multigestas (51,7 por ciento) y aunque hay similitud con la literatura mundial no se puede hablar de una relacion causa efecto ya que las P obtenidas fueron mayor de 0,05. A pesar de que porcentualmente la frecuencia de mantenimiento de relaciones sexuales se vio asociada al desarrollo de la infeccion la P (0,11) no demuestra significacion estadistica. Se concluyo que la clinica y el examen de orina simple patologico no son parametros estadisticamente significativos para el diagnostico de infecciones urinarias
Increasing Acceptance of Online Health Information: Understanding Barriers, Tailoring Messages and Self-Affirmation

The majority of deaths worldwide can now be attributed to so called, diseases of lifestyle, also known as non-communicable diseases (NCDs). These diseases are generally attributed to lifestyle behaviours, such as low fruit and vegetable consumption and lack of physical activity. Whilst historically NCDs have been known as diseases of the rich, current evidence shows that they are most prevalent in individuals with low socio-economic status. This thesis sets out to specifically address this issue by recruiting participants from a low SES population, in order to develop and test a web-based intervention addressing lifestyle behaviours. Initially the research identifies, and explores the barriers experienced by low SES individuals to leading healthy lifestyles, through interview data and further Q-sort analysis. The findings from these studies are used to tailor a health message, making it more pertinent to the target audience; the resulting message is incorporated into a website. The website is developed by understanding what constitutes a credible health related site in the eyes of the target audience. Initially the research identifies, and explores the barriers experienced by low SES individuals to leading healthy lifestyles, through interview data and further Q-sort analysis. The findings from these studies are used to tailor a health message, making it more pertinent to the target audience; the resulting message is incorporated into a website. The website is developed by understanding what constitutes a credible health related site in the eyes of the target audience. Self-affirmation theory (Steele, 1988) posits that reflecting on one’s positive attributes, reduces defensiveness to a potentially threatening health message. A recent study (Epton and Harris, 2008) suggests self-affirmation can lead to an actual change in the behaviour of individuals. This thesis replicates the work of Epton and Harris (2008) in an online environment, by demonstrating self-affirmed participants consumed more fruit and vegetable in the 7 days following exposure to the tailored website. These findings highlight the barriers experienced by low SES parents to adopting healthy lifestyles, and present a novel technique suitable for web-based interventions that has implications for a range of health related behaviours.
Distance Variations between Active Sites of H+-Pyrophosphatase Determined by Fluorescence Resonance Energy Transfer*

Homodimeric H+-pyrophosphatase (H+-PPase; EC 3.6.1.1) is a unique enzyme playing a pivotal physiological role in pH homeostasis of organisms. This novel H+-PPase supplies energy at the expense of hydrolyzing metabolic byproduct, pyrophosphate (PPi), for H+ translocation across membrane. The functional unit for the translocation is considered to be a homodimer. Its putative active site on each subunit consists of PPi binding motif, Acidic I and II motifs, and several essential residues. In this investigation structural mapping of these vital regions was primarily determined utilizing single molecule fluorescence resonance energy transfer. Distances between two C termini and also two N termini on homodimeric subunits of H+-PPase are 49.3 ± 4.0 and 67.2 ± 5.7 Å, respectively. Furthermore, putative PPi binding motifs on individual subunits are found to be relatively far away from each other (70.8 ± 4.8 Å), whereas binding of potassium and substrate analogue led them to closer proximity. Moreover, substrate analogue but not potassium elicits significant distance variations between two Acidic I motifs and two His-622 residues on homodimeric subunits. Taken together, this study provides the first quantitative measurements of distances between various essential motifs, residues, and putative active sites on homodimeric subunits of H+-PPase. A working model is accordingly proposed elucidating the distance variations of dimeric H+-PPase upon substrate binding.
Microfasciculation: a morphological pattern in leprosy nerve damage

Antunes S L G, Medeiros M F, Corte‐Real S, Jardim M R, da Costa Nery J A, Hacker M A V B, da Costa Valentim V, Amadeu T P & Sarno E N (2011) Histopathology58, 304–311
Down‐regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis

Sakamoto K, Aragaki T, Morita K‐i, Kawachi H, Kayamori K, Nakanishi S, Omura K, Miki Y, Okada N, Katsube K‐i, Takizawa T & Yamaguchi A (2011) Histopathology58, 531–542 Down‐regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis
Morbilidad de la extracción de los terceros molares en pacientes entre los 12 y 18 años de edad

Objective: An analysis is made of the incidence of complications following third molar surgical extraction in patients between 12 and 18 years of age. Patients and method: A retrospective study was conducted of 390 surgical extractions of upper and lower third molars in 173 patients operated upon under locoregional anesthesia during the year 2000 in the Master in Oral Surgery and Implantology of Barcelona University Dental School (Spain). The patients were divided into three age groups (A: 12-14 years, B: 15-16 years, C: 17-18 years). The reason for extraction, the degree of dental development, and third molar position, angle and impaction were recorded. Finally, the association of these variables to the appearance of postoperative complications was analyzed. Results: Most patients were females (66,9%), and the age group in which most extractions were carried out (62,8%) corresponded to Group C (17-18 years). The main reason for extraction was orthodontist indication (40,5%), closely followed by prophylaxis (39,5%). The existence of clinical manifestations was an indication for extraction in 20% of cases. The postoperative complications rate after the extraction of the global 390 molars was 15,6%. In Groups A, B and C the complication risks were 17,4%, 19% and 13,7%, respectively. All complications were reversible and of short duration. One case each of inferior alveolar nerve paresthesia and lingual nerve paresthesia was recorded, which subsided after one and two months, respectively. Conclusions: There were no significant differences in complications between the three age groups. An increased tendency towards complications was observed in females, and the percentage of postoperative problems increased.
Genetic composition of Brazilian population samples based on a set of twenty‐eight ancestry informative SNPs

Ancestry informative SNPs can be useful to estimate individual and population biogeographical ancestry. Brazilian population is characterized by a genetic background of three parental populations (European, African, and Brazilian Native Amerindians) with a wide degree and diverse patterns of admixture. In this work we analyzed the information content of 28 ancestry‐informative SNPs into multiplexed panels using three parental population sources (African, Amerindian, and European) to infer the genetic admixture in an urban sample of the five Brazilian geopolitical regions. The SNPs assigned apart the parental populations from each other and thus can be applied for ancestry estimation in a three hybrid admixed population. Data was used to infer genetic ancestry in Brazilians with an admixture model. Pairwise estimates of Fst among the five Brazilian geopolitical regions suggested little genetic differentiation only between the South and the remaining regions. Estimates of ancestry results are consistent with the heterogeneous genetic profile of Brazilian population, with a major contribution of European ancestry (0.771) followed by African (0.143) and Amerindian contributions (0.085). The described multiplexed SNP panels can be useful tool for bioanthropological studies but it can be mainly valuable to control for spurious results in genetic association studies in admixed populations. Am. J. Hum. Biol., 2010. © 2009 Wiley‐Liss, Inc.
CD133 expression is associated with small round blue cell tumour morphology in human central nervous system neoplasms

Schittenhelm J, Simon P, Harter P N, Zachskorn C, Schlaszus H, Röttger F, Winkels M, Weller M, Meyermann R & Mittelbronn M (2011) Histopathology58, 739–749 CD133 expression is associated with small round blue cell tumour morphology in human central nervous system neoplasms
Tissue remodelling in breast cancer: human mast cell tryptase as an initiator of myofibroblast differentiation

Mangia A, Malfettone A, Rossi R, Paradiso A, Ranieri G, Simone G & Resta L (2011) Histopathology58, 1096–1106 Tissue remodelling in breast cancer: human mast cell tryptase as an initiator of myofibroblast differentiation
Molecular characterization of sickle cell anemia in the Northern Brazilian state of Pará

To assess α+‐thalassemia deletion alleles, β‐thalassemia mutations and haplotypes linked to the HBB*S cluster in a sample of 130 unrelated sickle cell anemia (SCA) patients (55% female) from Belém, Pará State, for their possible effects on the patients' survival. ‐α3. 7, ‐α42, ‐α20. 5, and –MED α+‐thalassemia deletion alleles were investigated using multiplex gap‐PCR method. Characterization of β‐thalassemia mutations was made by direct genomic sequencing of the β‐globin gene amplified through polymerase chain reaction (PCR). Haplotypes were determined by analysis of six polymorphic restriction sites [(1) XmnI‐5′γG, (2) HindIII‐γG, (3) HindIII‐γA, (4) HincII‐ψβ, (5) HincII‐3′ψβ, and (6) HinfI‐5′β] followed by restriction digestion and agarose gel electrophoresis. Twenty‐one patients (16%) presented ‐α3. 7 thalassemia. Sixteen of those (76%) were heterozygous (‐α3.7/αα) and 5 (24%) were homozygous (‐α3.7/‐α3.7). ‐α4. 2, ‐α20. 5 and –MED deletions were not found. Nine cases of sickle cell‐β thalassemia were found and four different β‐thal mutations were identified: β+ −88 (C>T), 3.8%; β+ codon 24 (T > A), 1.5%; β+ IVSI‐110 (G > A), 0.7% and β (IVSI‐1 (G > A), 0.7%. No differences according to age were observed in ‐α3. 7 deletion, β‐thalassemia and HHB*S haplotypes distribution. Our results suggest that although α‐ and β‐thalassemia and βS haplotypes may have modulating effect on clinical expression and hematological parameters of SCA, these genetic variables probably have little influence on the subjects' survival. Am. J. Hum. Biol. 22:573–577, 2010. © 2010 Wiley‐Liss, Inc.
Insider Trades and Demand by Institutional and Individual Investors

There is a strong inverse relation between insider trading and institutional demand the same quarter and over the previous year. Our analysis suggests a combination of factors contribute to this relation. First, institutional investors are more likely to provide the liquidity necessary for insiders to trade. Second, insiders are more likely to buy low valuation and low lag return stocks while institutions are attracted to the opposite security characteristics. Last, the results are consistent with the hypothesis that insiders are more likely to view their securities as overvalued (undervalued) following a period when institutions were net buyers (sellers). The Author 2010. Published by Oxford University Press on behalf of The Society for Financial Studies. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org. , Oxford University Press.
Tumour infiltrating plasmacytoid dendritic cells in B cell post‐transplant lymphoproliferative disorders, human immunodeficiency virus‐associated B cell lymphomas and immune competent diffuse large B cell lymphomas

1. Restrepo MI, Vasquez EM, Echeverri C, Fiebelkorn KR, Anstead GM. Fibrin ring granulomas in Rickettsia typhi infection. Diagn. Microbiol. Infect. Dis. 2010; 66; 322–325. 2. Blanco P, Viallard JF, Parrens M, Mercie P, Pellegrin JL. Bone marrow fibrin ring granuloma. Lancet 2003; 362; 1224. 3. Nenert M, Mavier P, Dubuc N, Deforges L, Zafrani ES. Epstein–Barr virus infection and hepatic fibrin ring granulomas. Hum. Pathol. 1988; 19; 608–610. 4. Kang ES, Gong GY, Chi HS. A case of fibrin-ring granuloma in bone marrow and liver associated with Epstein–Barr virus infection. Korean J. Clin. Pathol. 1995; 15; 178–182. 5. Chung HJ, Chi HS, Cho YU, Jang S, Park CJ. Bone marrow fibrin ring granuloma: review of 24 cases. Korean J. Lab. Med. 2007; 27; 182–187. 6. Chung HJ, Jang S, Park CJ. Epstein–Barr virus infection associated with bone marrow fibrin ring granuloma. Am. J. Clin. Pathol. 2010; 133; 300–304.
A review of the biological and clinical characteristics of luminal‐like oestrogen receptor‐positive breast cancer

Habashy H O, Powe D G, Abdel‐Fatah T M, Gee J M W, Nicholson R I, Green A R, Rakha E A & Ellis I O (2012) Histopathology 60, 854–863 A review of the biological and clinical characteristics of luminal‐like oestrogen receptor‐positive breast cancer
Variability of the BCHE gene in Amerindians from Paraná, Brazil

This study aims to better understand the variability of the BCHE gene, and regions adjacent to it, in different populations. More specifically, it also aims to analyze the diversity of this DNA segment among two Amerindian populations, whose distinct characteristics provide us an extremely interesting material for genetic, evolutionary and anthropological studies, since they diverge genetically and culturally in spite of a very close living relationship in space and time.
Glucocorticoid treatment inhibits annexin-1 expression in rheumatoid arthritis CD4+ T cells.

OBJECTIVE Annexin-1 (Anx-A1) has been recently shown to play a key role in T-cell activation and to be highly expressed in T cells from RA patients. Here, we investigated the effects of glucocorticoids (GCs) on Anx-A1 expression in T cells in vitro and in vivo. METHODS To evaluate the effects of dexamethasone (Dex) on Anx-A1 expression, human peripheral blood T cells were incubated with Dex and then analysed by real-time PCR and western blotting. Similar experiments were carried out in vivo by measuring Anx-A1 levels in T cells from patients with RA before and after administration of steroids. RESULTS Incubation of T cells with Dex decreased Anx-A1 levels in a time-dependent fashion and almost abolished its expression after 12 h. Stimulation of T cells pre-incubated with Dex for 12 h with anti-CD3/CD28 led to significant reduction of IL-2 production. Addition of human recombinant Anx-A1 to Dex-treated cells reversed the inhibitory effects of the steroids on anti-CD3/CD28-induced IL-2 production. Treatment of RA patients with steroid decreased Anx-A1 expression in T cells. CONCLUSIONS GCs suppress Anx-A1 expression in T cells in vitro and in vivo. These results provide evidence for a novel pathway by which steroids regulate the adaptive immune response and suggest that Anx-A1 may represent a target for the treatment of autoimmune diseases.
Structure of Dimeric F1F0-ATP Synthase*

The structure of the dimeric ATP synthase from yeast mitochondria was analyzed by transmission electron microscopy and single particle image analysis. In addition to the previously reported side views of the dimer, top view and intermediate projections served to resolve the arrangement of the rotary c10 ring and the other stator subunits at the F0-F0 dimeric interface. A three-dimensional reconstruction of the complex was calculated from a data set of 9960 molecular images at a resolution of 27 Å. The structural model of the dimeric ATP synthase shows the two monomers arranged at an angle of ∼45°, consistent with our earlier analysis of the ATP synthase from bovine heart mitochondria (Minauro-Sanmiguel, F., Wilkens, S., and Garcia, J. J. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 12356–12358). In the ATP synthase dimer, the two peripheral stalks are located near the F1-F1 interface but are turned away from each other so that they are not in contact. Based on the three-dimensional reconstruction, a model of how dimeric ATP synthase assembles to form the higher order oligomeric structures that are required for mitochondrial cristae biogenesis is discussed.
Expression of deleted in malignant brain tumours 1 (DMBT1) relates to the proliferation and malignant transformation of hepatic progenitor cells in hepatitis B virus‐related liver diseases

Deng H, Gao Y‐B, Wang H‐F, Jin X‐L & Xiao J‐C (2012) Histopathology 60, 249–260 Expression of deleted in malignant brain tumours 1 (DMBT1) relates to the proliferation and malignant transformation of hepatic progenitor cells in hepatitis B virus‐related liver diseases