Miracle-dz/UniFAR-FTUs huggingface dataset:
Conditioned medium from human amniotic epithelial cells may induce the differentiation of human umbilical cord blood mesenchymal stem cells into dopaminergic neuron‐like cells

Dopaminergic (DA) neuron therapy has been established as a new clinical tool for treating Parkinson's disease (PD). Prior to cell transplantation, there are two primary issues that must be resolved: one is the appropriate seed cell origin, and the other is the efficient inducing technique. In the present study, human umbilical cord blood‐derived mesenchymal stem cells (hUCB‐MSCs) were used as the available seed cells, and conditioned medium from human amniotic epithelial cells (ACM) was used as the inducing reagent. Results showed that the proportion of DA neuron‐like cells from hUCB‐MSCs was significantly increased after cultured in ACM, suggested by the upregulation of DAT, TH, Nurr1, and Pitx3. To identify the process by which ACM induces DA neuron differentiation, we pretreated hUCB‐MSCs with k252a, the Trk receptor inhibitor of brain‐derived neurotrophic factor (BDNF) and nerve growth factor (NGF), and found that the proportion of DA neuron‐like cells was significantly decreased compared with ACM‐treated hUCB‐MSCs, suggesting that NGF and BDNF in ACM were involved in the differentiation process. However, we could not rule out the involvement of other unidentified factors in the ACM, because ACM + k252a treatment does not fully block DA neuron‐like cell differentiation compared with control. The transplantation of ACM‐induced hUCB‐MSCs could ameliorate behavioral deficits in PD rats, which may be associated with the survival of engrafted DA neuron‐like cells. In conclusion, we propose that hUCB‐MSCs are a good source of DA neuron‐like cells and that ACM is a potential inducer to obtain DA neuron‐like cells from hUCB‐MSCs in vitro for an ethical and legal cell therapy for PD. © 2013 Wiley Periodicals, Inc.
Impact of complexity upon tax compliance : a study of Australian personal taxpayers, The

This study explores the impact of complexity upon unintentional noncompliance behaviour for personal taxpayers in Australia. While compliance behaviour has been a topic of considerable research internationally over the last three decades or so, attention has generally been focused on intentional compliance behaviour, and in particular, on tax evasion and the processes by which taxpayers form their intentions.
Object Detection Using Multiple Level Annotations

Object Detection Using Multiple Level Annotations Mengmeng Xu Object detection is a fundamental problem in computer vision. Impressive results have been achieved on large-scale detection benchmarks by fully-supervised object detection (FSOD) methods. However, FSOD approaches require tremendous instance-level annotations, which are time-consuming to collect. In contrast, weakly supervised object detection (WSOD) exploits easily-collected image-level labels while it suffers from relatively inferior detection performance. This thesis studies hybrid learning methods on the object detection problems. We intend to train an object detector from a dataset where both instance-level and imagelevel labels are employed. Extensive experiments on the challenging PASCAL VOC 2007 and 2012 benchmarks strongly demonstrate the effectiveness of our method, which gives a trade-off between collecting fewer annotations and building a more accurate object detector. Our method is also a strong baseline bridging the wide gap between FSOD and WSOD performances. Based on the hybrid learning framework, we further study the problem of object detection from a novel perspective in which the annotation budget constraints are taken into consideration. When provided with a fixed budget, we propose a strategy for building a diverse and informative dataset that can be used to optimally train a robust detector. We investigate both optimization and learning-based methods to sample which images to annotate and which level of annotations (strongly or weakly supervised) to annotate them with. By combining an optimal image/annotation selection scheme with the hybrid supervised learning, we show that one can achieve the performance of a strongly super5 vised detector on PASCAL-VOC 2007 while saving 12.8% of its original annotation budget. Furthermore, when 100% of the budget is used, it surpasses this performance by 2.0 mAP percentage points.
Desnutrición infantil, una mirada desde diversos factores

El objetivo del estudio fue determinar los factores relacionados a la prevalencia de la desnutrición de niños menores de 3 años de la provincia de Huánuco. Fue un estudio observacional, prospectivo, transversal, analítico y correlacional; con una muestra aleatoria de 234 niños, a quienes se les aplicó fichas de valoración y a sus madres un cuestionario de factores; previamente validados y fiabilizados, según consideraciones éticas. Se realizó un análisis descriptivo e inferencial mediante la prueba Chi cuadrada de Pearson, y la prueba multinomial, para un p ≤ 0,05, apoyados en el SPSS V22. Se encontró relación significativa entre diferentes factores: desconocimiento de la madre del número de raciones que debe consumir el niño a partir del sexto mes, la inaccesibilidad económica para el abastecimiento de alimentos, la rotación de cultivos agrícolas tradicionales por comerciales ineficaces, el bajo peso al nacer (p<0,05), entre otros. En el análisis multivariado: la rotación de cultivos tradicionales por comerciales ineficaces, el niño con bajo peso al nacer y la deficiente calidad de los servicios materno-infantiles, aumentaron la probabilidad de tener desnutrición. Concluyendo que la desnutrición en niños menores de 3 años en estudio, está relacionada a factores económicos, ambientales, culturales, biológicos e institucionales.
Autopsy Findings in Patients on Postcardiotomy Extracorporeal Membrane Oxygenation (ECMO)

Objectives To assess the clinical sensitivity of causes of death, concomitant diseases and postoperative complications including thromboembolic events in ECMO patients. Methods: Between January 2000 and December 2004 154/202 patients (76.2%) died after postcardiotomy ECMO circulatory support. Autopsy was performed in 78 (50.6%) consecutive patients. Clinical and post-mortem data were prospectively recorded and compared concerning causes of death and postoperative complications including venous and arterial thromboembolisms and significant comorbidities. Results Mean age was 62.1±11.3 years, ejection fraction was 43.4±17.3%. 39.7% were emergency operations including acute coronary syndrome in 25.6% and preoperative cardiogenic shock in 28.2%. Successful ECMO weaning rate was 43.6%. Mean postoperative survival was 11.3 days. Premortem unknown concomitant diseases were found in 63 patients (80.8%) with clinical relevance in 9 patients (11.5%). Clinically unrecognized postoperative complications were found in 59 patients (75.6%) including acute cerebral infarction (n=7), acute bowel ischemia (1), intestinal perforation (3), pneumonia (4), venous thrombus formation (25) and systemic thromboembolic events (24). Clinically based causes of death were cardiac in 62.8%, multi-organ failure in 10.3%, cerebral in 5.1%, respiratory in 10.3%, fatal pulmonary embolism in 2.6%, technical in 5.1%, and others in 3.8%. Unexpected causes of death were found by autopsy in 22 patients (28.2%) including myocardial infarction (n=5), acute heart failure (4), fatal pulmonary embolism (2), pneumonia (2), ARDS (1), lung bleeding (1), fatal cerebrovascular event (4) and multiorgan failure (3). Conclusions In ECMO patients major discrepancies between clinical and post-mortem examination were found. The true incidence of thromboembolic events is highly underestimated by clinical evaluation.
Template‐Free Construction of Self‐Supported Sb Prisms with Stable Sodium Storage

Antimony (Sb) is a promising anode material for sodium‐ion batteries owing to its large capacity of 660 mAh g−1. However, its practical application is restricted by the rapid capacity decay resulted from a large volume expansion up to 390% upon Na alloying. Herein, construction of a self‐supported Sb array that has enough space allowing for effective accommodation of the volume change is reported. The array of Sb prisms is directly grown on a Cu substrate via a template‐free electrodeposition, followed by mild heating to consolidate the structural integrity between Sb and Cu. The resulting 3D architecture endows the Sb array with excellent sodium storage performance, exhibiting a reversible capacity of 578 mAh g−1 and retaining 531 mAh g−1 over 100 cycles at 0.5 C. The potential of Sb array in sodium‐ion full cells by pairing it with a Na0.67(Ni0.23Mg0.1Mn0.67)O2 cathode is further demonstrated. This full cell affords a specific energy of 197 Wh kg−1 at 0.2 C and a specific power of 1280 W kg−1 at 5 C. Considering its low cost and scale‐up capability, the template‐free route may find extensive applications in designing electrode architectures.
Analysis of cell‐to‐bubble attachment in sparged bioreactors in the presence of cell‐protecting additives

To investigate the mechanisms of cell protection provided by medium additives against animal cell injury in sparged bioreactors, we have analyzed the effect of various additives on the cell‐to‐bubble attachment process using CHO cells in suspension. Cell‐to‐bubble attachment was examined using three experimental techniques: (1) cell‐bubble induction time analysis (cell‐to‐bubble attachment times); (2) forming thin liquid films and observing the movement and location of cells in the thin films; and (3) foam flotation experiments. The induction times we measured for the various additives are as follows: no additive (50 to 500 ms), polyvinyl pyrrolidone (PVP: 20 to 500 ms), polyethylene glycol (PEG: 200 to 1000 ms), 3% serum (500 to 1000 ms), polyvinyl alcohol (PVA: 2 to 10 s), Pluronic F68 (5 to 20 s), and Methocel (20 to 60 s). In the thin film formation experiments, cells in medium with either F68, PVA, or Methocel quickly flowed out of draining thin liquid films and entered the plateau border. When using media with no additive or with serum, the flow of cells out of the thin liquid film and film drainage were slower than for media containing Pluronic F68. PVA, or Methocel. With PVP and PEG, the thin film drainage was much slower and cells remained trapped in the film. For the foam flotation experiments, a separation factor (ratio of cell concentration in the foam catch to that in the bubble column) was determined for the various additives. In the order of increasing separation factors (i.e., increasing cell attachment to bubbles), the additives are as follows: Methocel, PVA, Pluronic F68, 3% serum, serum‐free medium with no additives, PEG, and PVP. Based on the results of these three different cell‐to‐bubble attachment experiments, we have classified the cell‐protecting additives into three groups: (1) Pluronic F68, PVA, and Methocel (reduced cell‐to‐bubble attachment); (2) PEG and PVP (high or increased cell‐to‐bubble attachment); and (3) FBS (reduced cell attachment butslower drainage films compared with F68, PVA, and Methocel with some cell entrapment in those films). These phenomena are discussed in relation to the interfacial properties of the media reported in a companion Study (this issue). © 1995 John Wiley & Sons Inc.
Intention in motor learning through observation

The purpose of this experiment was to assess whether learning an action through observation is enhanced by the intention to reproduce the observed behaviour. Two groups of participants observed a model practise a timing task and performed a 24-hour delayed retention test. Participants in the first group of observers were explicitly instructed that they would be required to execute the timing task that they had observed as accurately as possible during the delayed retention test. Observers in the second group were instructed that they would be required to describe as accurately as possible the behaviour that they had observed. A control group of participants, who did not observe the model, was also administered the delayed retention test. The results of the retention test indicated that absolute timing (parameterization) was learned by the observers to the same extent with or without intention to reproduce the task. Indeed, on the retention test absolute timing for the two groups of observers was as effective as that for the models. However, observing with an intention to reproduce the task was beneficial for learning the movement's relative timing structure. Results are discussed with respect to a potential mechanism by which intention enhances observation.
A 250‐Year European Drought Inventory Derived From Ensemble Hydrologic Modeling

We present a 250‐year (1766–2015) inventory of European meteorological, hydrological, and agricultural droughts derived from ensemble simulations of the mesoscale Hydrological Model (mHM). The inventory of droughts takes into account an ensemble of 100 simulations from the hydrological model, allowing for assessment of how different meteorological forcing and model parameterizations affect a drought ranking. For the most extreme droughts, the variability in the ranking of drought events is low, while for the years with moderate precipitation deficits, the variability increases. Despite the underlying uncertainties, our drought inventory shows increased recurrence of soil moisture droughts in the Mediterranean and declining spatial extent of hydrological drought in Central Europe over the last three decades.
Identification and Expression Analysis of microRNAs at the Grain Filling Stage in Rice(Oryza sativa L.)via Deep Sequencing

MicroRNAs (miRNAs) have been shown to play crucial roles in the regulation of plant development. In this study, high-throughput RNA-sequencing technology was used to identify novel miRNAs, and to reveal miRNAs expression patterns at different developmental stages during rice (Oryza sativa L.) grain filling. A total of 434 known miRNAs (380, 402, 390 and 392 at 5, 7, 12 and 17 days after fertilization, respectively.) were obtained from rice grain. The expression profiles of these identified miRNAs were analyzed and the results showed that 161 known miRNAs were differentially expressed during grain development, a high proportion of which were up-regulated from 5 to 7 days after fertilization. In addition, sixty novel miRNAs were identified, and five of these were further validated experimentally. Additional analysis showed that the predicted targets of the differentially expressed miRNAs may participate in signal transduction, carbohydrate and nitrogen metabolism, the response to stimuli and epigenetic regulation. In this study, differences were revealed in the composition and expression profiles of miRNAs among individual developmental stages during the rice grain filling process, and miRNA editing events were also observed, analyzed and validated during this process. The results provide novel insight into the dynamic profiles of miRNAs in developing rice grain and contribute to the understanding of the regulatory roles of miRNAs in grain filling.
Short ReportConsanguineous marriage in Iran

Consanguineous marriage is a major feature of family systems in south-west Asia. The aim of the present study was to determine the current prevalence and patterns of consanguinity in Iran as a means of assessing the associated requirement for genetic counselling services. Consanguinity was studied in 12 ethnic/religious populations, the Persians (Shi’a and Sunni), Kurds (Shi’a and Sunni), Lurs, Azaris, Baluchis, Zabolis, Turkamans, Bakhtiaris, Ghashghais and Arabs. A multi-stage sampling design was used with a representative total sample of 306 343 couples. The overall rate of consanguineous marriage was 38.6% with a mean inbreeding coefficient (α) of 0.0185. First cousin marriages (27.9%) were the most common form of consanguineous union, with parallel patrilateral marriage especially favoured. Statistically significant differences were observed in the prevalence and patterns of consanguinity between ethnic/religious populations and geographical regions. There also were significant differences for proportions of consanguineous marriages between Shi’a and Sunni populations within the same ethnic group. The highest rates of consanguineous union were in the least affluent sections of the population.
Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification

This article describes the use of ultra scale‐down studies requiring milliliter quantities of process material to study the clarification of mammalian cell culture broths using industrial‐scale continuous centrifuges during the manufacture of a monoclonal antibody for therapeutic use. Samples were pretreated in a small high‐speed rotating‐disc device in order to mimic the effect on the cells of shear stresses in the feed zone of the industrial scale centrifuges. The use of this feed mimic was shown to predict a reduction of the clarification efficiency by significantly reducing the particle size distribution of the mammalian cells. The combined use of the rotating‐disc device and a laboratory‐scale test tube centrifuge successfully predicted the separation characteristics of industrial‐scale, disc stack centrifuges operating with different feed zones. A 70% reduction in flow rate in the industrial‐scale centrifuge was shown to arise from shear effects. A predicted 2.5‐fold increase in throughput for the same clarification performance, achieved by the change to a centrifuge using a feed zone designed to give gentler acceleration of the bioprocess fluid, was also verified at large‐scale. © 2006 Wiley Periodicals, Inc.
A Spike-Based Model of Neuronal Intrinsic Plasticity

The discovery of neuronal intrinsic plasticity (IP) processes which persistently modify a neuron's excitability necessitates a new concept of the neuronal plasticity mechanism and may profoundly influence our ideas on learning and memory. In this paper, we propose a spike-based IP model/adaptation rule for an integrate-and-fire (IF) neuron to model this biological phenomenon. By utilizing spikes denoted by Dirac delta functions rather than computing instantaneous firing rates for the time-dependent stimulus, this simple adaptation rule adjusts two parameters of an individual IF neuron to modify its excitability. As a result, this adaptation rule helps an IF neuron to keep its firing activity in a relatively “low but not too low” level and makes the spike-count distributions computed with adjusted window sizes similar to the experimental results.
Resting energy expenditure of morbidly obese patients using indirect calorimetry: a systematic review

The increasing proportion of acutely ill hospital patient admissions presenting with a morbidly obese body mass index (BMI ≥ 40 kg m−2) as a comorbidity is an emerging clinical concern. Suboptimal food intake and malnutrition is prevalent in the acute care hospital setting. The energy requirements necessary to prevent malnutrition in acutely ill patients with morbid obesity remains unclear. The aim of this systematic review was to identify studies in the literature that have used indirect calorimetry to measure the resting energy expenditure of patients with morbid obesity to establish their minimum energy requirements and the implications for optimal feeding practices in acutely ill hospitalized patients. A total of 20 studies from PubMed, Cochrane Library and Embase met the inclusion criteria and were reviewed. All articles were graded using the Australian National Health and Medical Research Council levels of evidence and given a quality rating using the American Dietetic Association recommendations. Studies were categorized according to the mean BMI of its subjects. The most commonly measured resting energy expenditures for morbidly obese patients are between 2,000 and 3,000 kcal d−1 (8,400–12,600 kJ d−1). Activity and injury factors of acutely ill morbidly obese patients could result in significantly greater energy requirements for this patient group and are unlikely to be met by standard hospital menus. Establishing the minimum energy requirements for this population group will help inform adequate and accurate energy provision in the acute setting. Outcomes of underfeeding and overfeeding in morbidly obese patients warrant further research.
Time Domain NMR in Polymer Science: From the Laboratory to the Industry

Highly controlled polymers and nanostructures are increasingly translated from the lab to the industry. Together with the industrialization of complex systems from renewable sources, a paradigm change in the processing of plastics and rubbers is underway, requiring a new generation of analytical tools. Here, we present the recent developments in time domain NMR (TD-NMR), starting with an introduction of the methods. Several examples illustrate the new take on traditional issues like the measurement of crosslink density in vulcanized rubber or the monitoring of crystallization kinetics, as well as the unique information that can be extracted from multiphase, nanophase and composite materials. Generally, TD-NMR is capable of determining structural parameters that are in agreement with other techniques and with the final macroscopic properties of industrial interest, as well as reveal details on the local homogeneity that are difficult to obtain otherwise. Considering its moderate technical and space requirements of performing, TD-NMR is a good candidate for assisting product and process development in several applications throughout the rubber, plastics, composites and adhesives industry.
A World of Contrasted but Interdependent Inequality Regimes: The Latin America Paradox

ABSTRACT The previous issue of this journal published an explanation of three contemporary paradoxes: dramatically increased inequalities in China despite economic development reducing poverty; the excessively large costs incurred by the state following a surge of inequality in the finance-led growth regime of the United States (US); and, within Europe, some social democratic countries continue to exhibit a complementarity between and extended welfare system, more moderate inequalities and a dynamic innovation and production system. This analysis concluded that the US, Chinese and European inequality regimes are different but they express complementary growth patterns. Applying the same socio-economic approach, based upon the concept of inequality regimes, this article addresses another contemporary paradox. Latin America, previously the continent with the highest inequality, has reversed the former dynamics to exhibit a growth pattern based upon inequality reduction, while still relying heavily upon a strong international demand for commodities. This analysis investigates the durability and likelihood of the Latin American U-turn and concludes that there is a possible alternative to the hypothesis of an irreversible globalization of inequality because China, North America, Europe and Latin America do not follow the same trajectory, having developed contrasting regimes of inequality that co-evolve and are largely complementary at the global level. Consequently the future of more inclusive Latin American (and other) economies depends on the interaction between new domestic democratic advances and the reconfiguration of the international economy.