Miracle-dz/UniFAR-FTUs huggingface dataset:
Measurement of Telomere Length: A New Assay Using QuantiGene Chemistry on a Luminex Platform

Background: Telomeres are tandem repeats of sequences present at the end of the chromosomes that maintain chromosomal integrity. After repeated cell division, telomeres shorten to a critical level, triggering replicative senescence or apoptosis, which is a key determinant of cellular aging. Short telomeres also contribute to genome instability and are a hallmark of many cancers. There are several methods for estimating telomere length (TL) from extracted DNA samples. Southern blot is accurate but requires a large quantity of DNA and is expensive. qPCR is cost-effective and requires a small quantity of DNA and is therefore widely used for large-scale epidemiologic studies; however, it typically requires triplicates. We describe a novel multiplexed probe-based non-PCR method for TL measurement. Methods: A small amount of DNA (∼50 ng) is hybridized to telomere repeat sequence–specific probes (T) and a reference single gene probes (R). T and R signals are detected from a single reaction well containing the same input DNA. Branching DNA technology is used to amplify the signal, which is detected by Luminex technology. Results: The intra- and interassay CV (∼3% and ∼5%, respectively) shows the precision of the new assay and the measurements from single well correlated well with traditional single-plex qPCR run in triplicate (r = 0.7 to 0.8). The assay was also validated in an independent set of samples using Southern blot (r = 0.74). Conclusion: We describe a novel assay for TL assessment using the Luminex platform. Impact: This may offer an alternative cost-efficient way to study TL in extracted DNA samples. See all the articles in this CEBP Focus section, “Biomarkers, Biospecimens, and New Technologies in Molecular Epidemiology. ” Cancer Epidemiol Biomarkers Prev; 23(12); 2667–72. ©2014 AACR.
Sensor data fusion of LIDAR with stereo RGB-D camera for object tracking

In Industry 4.0 scenarios, autonomously navigating robots will have to perform dedicated tasks in controlled environments, such as production halls or storage facilities. In the presence of pedestrians and other dynamic objects, robust collision detection is imperative in order to avoid harm of human or material. Supplementary sensors as part of the infrastructure may provide additional real-time overview. In this paper, a concept for dynamic object tracking by sensor data fusion, using a stationary stereo camera and a laser range finder on a mobile platform, is presented and analyzed. The proposed approach consists of two modules that involve frame-to-frame detection of targets, as well as subsequent data association, fusion and tracking. Object detection is carried out by 3D-processing techniques on point clouds. Data association for multi-target tracking is achieved, using the Joint Probabilistic Data Association Filter (JPDAF). Combination of sensor information is done by a hierarchical data fusion approach. Experiments show that this improves robustness to occlusions or sensor failure significantly.
Changes in the compound action current amplitudes in relation to the conduction velocity and functional recovery in the reconstructed peripheral nerve

The average axon diameter in the proximal segment of a transected and reconstructed peripheral nerve will decrease shortly after the transection and increase again when the regenerating axons make contact with their targets. The magnetically recorded nerve compound action current (NCAC) amplitude and the conduction velocity (CV) are directly related to the axon diameters. In this experiment, the peroneal nerve was unilaterally transected and reconstructed in 42 rabbits. After 3, 4.5, 6, 8, 12, 20, and 36 weeks of regeneration time, hind leg motor function recovery, NCAC amplitude, and CV1st peak were studied. Our results demonstrate a significant decrease in signal amplitude and CV in the first 8 weeks after reconstruction. These decreases are related (P < 0.05). After 8 weeks of regeneration time, motor function and the CV of the recorded signals start to recover, but the signal amplitudes do not. Based on the correlation of the CV and signal amplitude with axon diameter, they would both be expected to increase with recovering function. As an explanation for this lack of increase of signal amplitude, we suggest that, at the same time as some axons reach their target organs and start to mature, a number of the axons which have not reached a proper target organ will lose their signal‐conducting capability. This will cause a decrease in compound signal amplitude, which cancels out the expected increase in NCAC amplitude, due to axonal maturation. © 1999 John Wiley & Sons, Inc. Muscle Nerve 22: 1087–1093, 1999
Pseudoaneurysm of the femoral artery after cardiac catheterisation: diagnosis and treatment by manual compression guided by Doppler colour flow imaging.

OBJECTIVE--To assess the value of Doppler colour flow imaging for diagnosing and guiding non-surgical treatment of pseudoaneurysm of the femoral artery complicating cardiac catheterisation. DESIGN--A prospective study. SETTING--Cardiac department in a teaching hospital. PATIENTS--9 patients (8 female, 1 male) who presented with pseudoaneurysm 1-15 days after cardiac catheterisation. INTERVENTIONS--The femoral arterial communication to the false aneurysm was localised by Doppler colour flow imaging. Manual pressure was then applied to the ultrasound transducer which was positioned directly over the site of the arterial communication. Pressure was progressively increased until it was sufficient to prevent colour flow from the artery into the false aneurysm cavity while allowing Doppler flow to continue within the arterial lumen. MAIN OUTCOME MEASURES--Characteristics of pseudoaneurysm, duration of manual compression, success rate, follow up. RESULTS--The pseudoaneurysms ranged from 1.3 to 5.5 cm in length. Six pseudoaneurysms were 1.3-2.0 cm away from the arterial puncture. The pseudoaneurysm was closed in 8/9 patients by compression exerted manually through the transducer for 25-40 minutes (3 successful cases required two or three periods of compression within 48 hours). No pseudoaneurysm recurred during 14-61 days of follow up. CONCLUSIONS--Most pseudoaneurysms of the femoral artery can be treated by a period of manual pressure applied with an ultrasound transducer and guided by Doppler colour flow.
Fusimotor activity in masseter nerve of the cat during reflex jaw movements.

1. Unit recordings have been made from the central ends of filaments of the masseter nerve in lightly anaesthetized cats. Evidence is presented to show that fusimotor activity may be distinguished from alpha motor activity. 2. During reflex cyclic movements induced by intra‐oral stimulation, two distinct patterns of fusimotor firing emerged. One type of unit increased firing at the beginning and sustained this with little modulation throughout the movements. The other type was strongly modulated approximately in parallel with the alpha motor activity. 3. By comparison with records of jaw elevator spindle afferents under similar conditions, it was deduced that the sustained type of action was due to dynamic fusimotor neurones while the modulated type was due to static fusimotor neurones. 4. The patterns of fusimotor activity seen in these rhythmic movements under light anaesthesia agree well with the patterns deduced from spindle recordings in the conscious cat during mastication. 5. The results emphasize the importance of looking beyond a simple hypothesis of ‘alpha‐gamma co‐activation’ to explain fusimotor function. It is proposed that tonic dynamic fusimotor activity is set at the beginning of a movement to determine the incremental sensitivity of primary endings to stretch. The static fusimotor fibres are activated principally during shortening to help keep both primary and secondary endings active.
Parkinson's disease, stroke, and related epidemiology

We investigated the prevalence of cerebrovascular disease and other comorbidities in Parkinson's disease (PD) patients compared to the general population. Five hundred PD patients were chosen randomly from one author's (A.H.R.) database. Age‐ and sex‐matched controls were derived from 270 patients with essential tremor from the same database and from 490 patients in a general practitioner's database. Age, hypertensive status, smoking status, coronary artery disease, orthostatic hypotension, diabetes mellitus, and symptomatic cerebrovascular disease (stroke or transient ischemic attack) were assessed. Statistical analysis was performed using Pearson χ2 testing and binary logistic regression analysis. The prevalence of coronary artery disease, hypertension, diabetes mellitus, and orthostatic hypotension was similar among groups. The PD group had more patients who never smoked and less current smokers than the other groups. While there were similar frequencies of symptomatic cerebrovascular disease among groups, the prevalence of stroke was lower in PD patients. This difference disappeared upon stratification into groups based on smoking status and in the addition of smoking as a covariate in the multivariate analysis. Diminished smoking in PD patients likely plays a role in our finding of decreased stroke in patients with PD. Increased access to appropriate neurological care and subsequent prevention of stroke after a warning transient ischemic attack may also play a role, as may diminished levels of excitotoxic neurotransmitters in PD patients. © 2005 Movement Disorder Society
Orbital exenteration: The 10-year Massachusetts Eye and Ear Infirmary experience

ABSTRACT The authors report their experience with orbital exenteration surgery at one academic institution over a 10-year period and review the literature. This retrospective cohort study monitored outcomes of all patients who underwent orbital exenteration surgery at Massachusetts Eye and Ear Infirmary between January 2003 and January 2013. Patients with no follow-up data or survival data were excluded from the study. The main outcome measures were surgical complications, disease status of surgical margins, need for adjuvant treatment, local recurrence, metastases and survival. 23 patients with malignancy and 2 with mucormycosis met inclusion criteria for the study. Surgical procedures included non-lid sparing total exenteration (44%), lid-sparing total exenteration (32%), non-lid sparing partial exenteration (8%) and lid-sparing partial exenteration (16%). 44% underwent additional extra-orbital procedures. Survival rates were 72% at 1 year, 48% at 3 years, and 37% at 5 years. Of patients with malignancies, 48% had clear margins after exenteration. There was no statistically significant difference in survival between patients with negative surgical margins compared to positive margins (p = 0.12). Mortality was highest in patients with melanoma (85.7%) and lowest in patients with non-squamous cell lid malignancies (0%). Our study suggests that the type of disease has a much greater impact on the survival of patients undergoing exenteration surgery than the type of exenteration surgery or the disease status of surgical margins. Patients with non-squamous cell lid malignancies and localized orbital disease have the best prognosis for tumor eradication from this radical and highly disfiguring surgery.
Separase is Required at Multiple Pre-Anaphase Cell Cycle Stages in Human Cells

The yeast separase proteins Esp1 and Cut1 are required for loss of sister chromatid cohesion that occurs at the moment of anaphase onset. Circumstantial evidence has linked human separase to centromere separation at anaphase, but a direct test that the role of this enzyme is functionally conserved with the yeast proteins is lacking. Here we describe the effects of separase depletion from human cells using RNA interference. Surprisingly, HeLa cells lacking separase are delayed or arrest at the G2/M phase transition. This arrest is not likely due to the activation of a known checkpoint control, but may be a result of a failure to construct a mitotic chromosome. Without separase, cells also have a prolonged prometaphase, perhaps resulting from defects in spindle assembly or dynamics. In cells that reach mitosis, sister arm resolution and separation are perturbed, whereas in anaphase cells sister centromeres do appear to separate. These data indicate that separase function is not restricted to anaphase initiation and that its role in promoting loss of sister chromatid cohesion might be preferentially at arms but not centromeres.
Clinical Implications of Hepatic Preservation Injury After Adult Liver Transplantation

Several advances in organ preservation have allowed for improved results after liver transplantation; however, little information is available regarding the clinical impact of preservation injury on the postoperative course. The medical records of 889 liver transplants were retrospectively reviewed. Preservation injury was classified according to postoperative aspartate aminotransferase values as minor (<1000 U/L), moderate (1000–5000 U/L), or severe (>5000 U/L). The following criteria were analyzed and compared according to the extent of preservation injury: patient and graft survival, retransplantation rate, duration of hospitalization and postoperative ventilation, as well as incidence of rejection, infection, and hemodialysis. The majority of patients received a liver with minor preservation injury (75.9%), whereas 22.7% and 1.3% of grafts showed moderate or severe injury. Graft survival was significantly lower in patients with severe preservation injury, when compared to minor or moderate injury. The relative risk for initial nonfunction was 39.36‐fold increased (95% confidence interval (ci): 10.3–150.2), as it was increased for duration of postoperative ventilation (6.92‐fold; 95%ci: 2.1–22.3) and hemodialysis (6.13‐fold; 95%ci: 1.9–19.3). Since the incidence of retransplantation was significantly increased (50%), patient survival remained comparable between all groups. Severe preservation injury had a tremendous impact on the postoperative clinical course, requiring the maximum medical effort to achieve adequate patient survival.
Answering clinical questions.

BACKGROUND Physicians often have unanswered clinical questions. The purpose of this study was to determine how often the answers to these questions can be found in the medical literature. METHODS We collected unanswered clinical questions from family physicians at the end of clinical half-days. The authors and medical librarians then used textbooks and MEDLINE to find answers to each question. We returned to the physicians one to five selected references for each question. Each physician rated these sources on how well they answered the questions and how they might influence the physician's practice. RESULTS One hundred three questions were gathered. Physicians asked an average of 0.5 questions per half-day. We searched for answers to 86 questions, and the physicians returned ratings for 84. Forty-five (54%) of these questions were fully or nearly fully answered by the materials returned to the physicians. Of the questions for which answers were found, MEDLINE searches accounted for 71%; textbooks, 20%; and a combination, 9%. MEDLINE searches took an average of 27 minutes, whereas textbook searches averaged 6 minutes. CONCLUSIONS The medical literature can provide answers to a majority of clinical questions; however, finding these answers is time-consuming and expensive. Physicians need more efficient ways to answer their clinical questions.
Temperature niche shift observed in a Lepidoptera population under allochronic divergence

A process of adaptive divergence for tolerance to high temperatures was identified using a rare model system, consisting of two sympatric populations of a Lepidoptera (Thaumetopoea pityocampa) with different life cycle timings, a ‘mutant’ population with summer larval development, Leiria SP, and the founder natural population, having winter larval development, Leiria WP. A third, allopatric population (Bordeaux WP) was also studied. First and second instar larvae were experimentally exposed to daily‐cycles of heat treatment reaching maximum values of 36, 38, 40 and 42 °C; control groups placed at 25 °C. A lethal temperature effect was only significant at 42 °C, for Leiria SP, whereas all temperatures tested had a significant negative effect upon Leiria WP, thus indicating an upper threshold of survival c.a. 6 °C above that of the WP. Cox regression model, for pooled heat treatments, predicted mortality hazard to increase for Leiria WP (+108%) and Bordeaux WP (+78%) in contrast to Leiria SP; to increase by 24% for each additional °C; and to decrease by 53% from first to second instar larvae. High variability among individuals was observed, a population characteristic that may favour selection and consequent adaptation. Present findings provide an example of ecological differentiation, following a process of allochronic divergence. Results further contribute to a better understanding of the implications of climate change for ecological genetics.
The Economic Consequences of the 2018 US-China Trade Conflict: A CGE Simulation Analysis

This paper aims at evaluating the economic consequences of the 2018 US-China trade conflict. The potential impact of the proposed tariff increases is calculated using a global CGE model. Capital deepening and technological spillover induced by trade are also taken into account to explore the long-run influence. We can derive the following implications.First, the additionally imposed tariffs on goods alone declines the GDP in the US and China by 0.1% and 0.2%, respectively. The equivalent variation in the US and China is reduced by 9.8 billion and 35.2 billion USD, respectively. Although other countries gain from trade diversion, losses exceed gains globally.Second, considering the effect from capital deepening and technological spillover induced by trade makes the situation worse. The GDP in the US and China declines by 1.6% and 2.5%, respectively. The equivalent variation in the US and China is reduced by 199.5 billion USD and 187.1 billion USD, respectively. Again, the trade diversion is not large enough to recover losses in these countries.Third, the imposed tariffs distort relative prices, resulting in changes to the global production structure. Both the US and China lose their comparative advantage in transport, electronic, and machinery equipment production, while other countries expand their production in these sectors.Finally, China’s retaliatory tariff increases worsens the US economy to some extent, but it comes at a cost to the Chinese economy. In the long run, retaliation is not an appropriate policy response
Commemorating 1917 in Russia: Ambivalent State History Policy and the Church’s Conquest of the History Market

Abstract Russia’s historical policy towards the centenary of 1917 was composed of several parallel strategies: diminishing the meaning of the event to avoid the head of state and other government figures having to take a stance; outsourcing commemorative events, with no pre-planned grand design; developing a reconciliatory narrative of the ‘Whites’ and the ‘Reds’; and allowing other actors to promote a plurality of contradictory readings of the events. Yet the space left by the state’s refusal to commemorate 1917 has been taken over by the Church, which, as today’s most active engineer of Russia’s historical policy, promoted a very clear pro-Tsarist narrative best embodied by the multimedia historical park ‘Russia—my history’ (Rossiya—moya istoriya).
Quantum-inspired immune clonal clustering algorithm based on watershed

Based on the concepts and principles of quantum computing, a novel clustering algorithm, called a quantum-inspired immune clonal clustering algorithm based on watershed (QICW), is proposed to deal with the problem of image segmentation. In QICW, antibody is proliferated and divided into a set of subpopulation groups. Antibodies in a subpopulation group are represented by multi-state gene quantum bits. In the antibody's updating, the quantum mutation operator is applied to accelerate convergence. The quantum recombination realizes the information communication between the subpopulation groups so as to avoid premature convergences. In this paper, the segmentation problem is viewed as a combinatorial optimization problem, the original image is partitioned into small blocks by watershed algorithm, and the quantum-inspired immune clonal algorithm is used to search the optimal clustering centre, and make the sequence of maximum affinity function as clustering result, and finally obtain the segmentation result. Experimental results show that the proposed method is effective for texture image and SAR image segmentation, compared with the genetic clustering algorithm based on watershed (W-GAC), and the k-means algorithm based on watershed (W-KM).
Barriers to and facilitators for female participation in an HIV prevention project in Rural Ethiopia: Findings from a qualitative evaluation

Ethiopian women face complex social and cultural factors that influence their probability of HIV infection. HIV prevention efforts among this population are particularly important; however, female participation in a rural, HIV prevention project has been minimal. This programme evaluation investigated barriers and facilitators influencing women's ability to participate in project activities. Evaluation data were collected through nine focus groups and 20 semi‐structured interviews, which were conducted between October and November 2003. The main themes found to negatively influence women's decisions to participate in this HIV prevention activity included: domestic workloads, lack of education and awareness, and cultural norms that have discouraged discussions about HIV and sexuality. Domestic chores, which are labour intensive and limit time and energy, were found to be the primary barrier to participation among women. Respondents also indicated that female illiteracy and limited educational attainment occur within a social context that traditionally supports education for men but discourages formal knowledge among women, including HIV prevention. Lack of education and inability to freely discuss sexuality denies women access to health information, potentially exposing women to adverse consequences such as HIV infection. Identified facilitators of participation included a radio serial drama and the one female peer educator associated with the project.