Miracle-dz/UniFAR-FTUs huggingface dataset:
Lack of contribution of nitric oxide synthase to cholinergic vasodilation in murine renal afferent arterioles.

We have previously reported significant increases in neuronal nitric oxide synthase (NOS) immunostaining in renal arterioles of angiotensin type 1A receptor (AT1A) knockout mice, and in arterioles and macula densa cells of AT1A/AT1B knockout mice. The contribution of nitric oxide derived from endothelial and macula densa cells in the maintenance of afferent arteriolar tone and acetylcholine-induced vasodilation was functionally determined in kidneys of wild-type, AT1A, and AT1A/AT1B knockout mice. Acetylcholine-induced changes in arteriolar diameters of in vitro blood-perfused juxtamedullary nephrons were measured during control conditions, in the presence of the nonspecific NOS inhibitor, Nω-nitro-l-arginine methyl ester (NLA), or the highly selective neuronal NOS inhibitor, N5-(1-imino-3-butenyl)-l-ornithine (VNIO). Acetylcholine (0.1 mM) produced a significant vasoconstriction in afferent arterioles of AT1A/AT1B mice (-10.9 ± 5.1%) and no changes in afferent arteriolar diameters of AT1A knockout mice. NLA (0.01-1 mM) or VNIO (0.01-1 μM) induced significant dose-dependent vasoconstrictions (-19.8 ± 4.0% 1 mM NLA; -7.8 ± 3.5% 1 μM VNIO) in afferent arterioles of kidneys of wild-type mice. VNIO had no effect on afferent arteriole diameters of AT1A knockout or AT1A/AT1B knockout mice, suggesting nonfunctional neuronal nitric oxide synthase. These data indicate that acetylcholine produces a significant renal afferent arteriole vasodilation independently of nitric oxide synthases in wild-type mice. AT1A receptors are essential for the manifestation of renal afferent arteriole responses to neuronal nitric oxide synthase-mediated nitric oxide release.
Endoscopic Staple‐Assisted Esophagodiverticulostomy: An Excellent Treatment of Choice for Zenker's Diverticulum

Objectives The objectives of the present study are to review the technique of endoscopic staple‐assisted esophagodiverticulostomy (ESED) for the treatment of Zenker's diverticulum and to describe our e‐perience and modifications with ESED and the advantages of ESED over previous treatments of Zenker's diverticulum.
Reactivity of Genotype-Specific Recombinant Proteins of Human Erythrovirus B19 with Plasmas from Areas Where Genotype 1 or 3 Is Endemic

ABSTRACT Human erythrovirus (parvovirus) B19 (B19) is a common human pathogen. The recent discovery of three genotypes, 1 to 3, raised issues related to the ability of genotype-specific antigens to cross-react with antibodies elicited by other genotypes. This study assessed antibody capture and immunoglobulin G (IgG) cross-reactivity between genotype 1 and genotype 3 recombinant antigens and analyzed the potential gain of adding VP1 protein to VP2 capsid antigen. Plasma samples from genotype 1- or genotype 3-infected populations were blindly tested with blindly prepared reagents. The IgG reactivity was assessed with baculovirus-expressed VP2 or VP1 and VP2 recombinant genotype 1 or genotype 3 proteins in a standardized enzyme immunoassay (EIA). A high degree of agreement (>95%) between EIA results was observed, with Spearman correlation coefficient and kappa reliability coefficient results of ≥0.95 for samples from the United Kingdom and ≥0.77 for samples from Ghanaian children, respectively. Most discrepant results were related to equivocal reactivity. The addition of VP1 to VP2 capsids did not significantly impact antibody detection. These data suggest that the currently available genotype-1-based IgG EIA is suitable to detect antibody to B19 genotype 3 in Ghanaian children. However, samples from the Ghanaian adult population exhibited atypical results in the assay, possibly due to the high levels of nonspecific IgG antibodies found in adults living in this region. Within these limitations, the study demonstrates that genotype 1 and genotype 3 antigens are equally effective in detecting either antibody species.
Evaluation of Learners' Adjustment of Question Difficulty in Adaptive Practice of Facts

Personalized educational systems are able to provide learners questions of specified difficulty. Since learners differ, the appropriate level of difficulty may vary and it may be impossible to find an universal setting. We implemented a version of an adaptive educational system for geography practice that allows learners to adjust difficulty of questions. We evaluated this feature using a randomized control experiment. The overall results show only a small effect of the adjustment. A more detailed analysis, however, shows that for some groups of learners the effect can be important, although not necessarily advantageous. The collected data from the experiment provide insight into how to tune question difficulty automatically.
Evaluation of an extracellular matrix-derived acellular biphasic scaffold/cell construct in the repair of a large articular high-load-bearing osteochondral defect in a canine model.

BACKGROUND Osteochondral lesion repair is a challenging area of orthopedic surgery. Here we aimed to develop an extracellular matrix-derived, integrated, biphasic scaffold and to investigate the regeneration potential of the scaffold loaded with chondrogenically-induced bone marrow-derived mesenchymal stem cells (BMSCs) in the repair of a large, high-load-bearing, osteochondral defect in a canine model. METHODS The biphasic scaffolds were fabricated by combining a decellularization procedure with a freeze-drying technique and characterized by scanning electron microscopy (SEM) and micro-computed tomography (micro-CT). Osteochondral constructs were fabricated in vitro using chondrogenically-induced BMSCs and a biphasic scaffold, then assessed by SEM for cell attachment. Osteochondral defects (4.2 mm (diameter) × 6 mm (depth)) were created in canine femoral condyles and treated with a construct of the biphasic scaffold/chondrogenically-induced BMSCs or with a cell-free scaffold (control group). The repaired defects were evaluated for gross morphology and by histological, biochemical, biomechanical and micro-CT analyses at 3 and 6 months post-implantation. RESULTS The osteochondral defects of the experimental group showed better repair than those of the control group. Statistical analysis demonstrated that the macroscopic and histologic grading scores of the experimental group were always higher than those of the control group, and that the scores for the experimental group at 6 months were significantly higher than those at 3 months. The cartilage stiffness in the experimental group (6 months) was (6.95 ± 0.79) N/mm, 70.77% of normal cartilage; osteochondral bone stiffness in the experimental group was (158.16± 24.30) N/mm, 74.95% of normal tissue; glycosaminoglycan content of tissue-engineered neocartilage was (218 ± 21.6) µg/mg (dry weight), 84.82% of native cartilage. Micro-CT analysis of the subchondral bone showed mature trabecular bone regularly formed at 3 and 6 months, with no significant difference between the experimental and control groups. CONCLUSION The extracellular matrix-derived, integrated, biphasic scaffold shows potential for the repair of large, high-load-bearing osteochondral defects.
Representative Multiple Kernel Learning for Classification in Hyperspectral Imagery

Recently, multiple kernel learning (MKL) methods have been developed to improve the flexibility of kernel-based learning machine. The MKL methods generally focus on determining key kernels to be preserved and their significance in optimal kernel combination. Unfortunately, computational demand of finding the optimal combination is prohibitive when the number of training samples and kernels increase rapidly, particularly for hyperspectral remote sensing data. In this paper, we address the MKL for classification in hyperspectral images by extracting the most variation from the space spanned by multiple kernels and propose a representative MKL (RMKL) algorithm. The core idea embedded in the algorithm is to determine the kernels to be preserved and their weights according to statistical significance instead of time-consuming search for optimal kernel combination. The noticeable merits of RMKL consist that it greatly reduces the computational load for searching optimal combination of basis kernels and has no limitation from strict selection of basis kernels like most MKL algorithms do; meanwhile, RMKL keeps excellent properties of MKL in terms of both good classification accuracy and interpretability. Experiments are conducted on different real hyperspectral data, and the corresponding experimental results show that RMKL algorithm provides the best performances to date among several the state-of-the-art algorithms while demonstrating satisfactory computational efficiency.
A theoretical framework to support research of health service innovation.

OBJECTIVE Health service managers and policy makers are increasingly concerned about the sustainability of innovations implemented in health care settings. The increasing demand on health services requires that innovations are both effective and sustainable; however, research in this field is limited, with multiple disciplines, approaches and paradigms influencing the field. These variations prevent a cohesive approach, and therefore the accumulation of research findings, in the development of a body of knowledge. The purpose of this paper is to provide a thorough examination of the research findings and provide an appropriate theoretical framework to examine sustainability of health service innovation. METHODS This paper presents an integrative review of the literature available in relation to sustainability of health service innovation and provides the development of a theoretical framework based on integration and synthesis of the literature. RESULTS A theoretical framework serves to guide research, determine variables, influence data analysis and is central to the quest for ongoing knowledge development. This research outlines the sustainability of innovation framework; a theoretical framework suitable for examining the sustainability of health service innovation. CONCLUSION If left unaddressed, health services research will continue in an ad hoc manner, preventing full utilisation of outcomes, recommendations and knowledge for effective provision of health services. The sustainability of innovation theoretical framework provides an operational basis upon which reliable future research can be conducted.
The Roles of PAX6 and SOX2 in Myopia: lessons from the 1958 British Birth Cohort.

PURPOSE Myopia is a common complex trait that affects up to 60% of some populations. Its development is influenced by multiple genes and environmental factors. PAX6 and SOX2 are genes with fundamental roles in ocular growth and development, and they have been linked with myopia in a recent linkage study. The authors investigated the roles of PAX6 and SOX2 in common myopia as part of a broader association study of refractive error. METHODS Five hundred ninety-six persons from the 1958 British Birth Cohort, a nationally representative population, were randomly selected from the outer tertiles of the refractive error (RE) distribution and were genotyped using 25 tagSNPs across PAX6 and 3 tagSNPs across SOX2 and their putative control regions. This experiment had 80% power to exclude either gene contributing more than 10% of the variance of refractive error. RESULTS All SNPs were in Hardy-Weinberg equilibrium, and the genotyping failure rate was less than 5%. Accounting for multiple testing, no significant association (P < 0.05) was found between any of the SNPs or haplotypes and refractive error. CONCLUSIONS PAX6 and SOX2 are obvious candidates in RE genetic studies because of their biological roles and prior linkage studies. The present findings strongly suggest refractive error is not directly affected in this population by variants in either gene or by their known promoters/enhancers. The authors suggest that neither PAX6 nor SOX2 should be prioritized in the international search for genetic modifiers of refractive error. Their findings contribute to broader understanding of the pathophysiology of refractive error and highlight the critical role of replication in genetic research on complex disorders.
Adult intussusception.

OBJECTIVE The objectives were to review adult intussusception, its diagnosis, and its treatment. SUMMARY BACKGROUND DATA Adult intussusception represents 1% of all bowel obstructions, 5% of all intussusceptions, and 0.003%-0.02% of all hospital admissions. Intussusception is a different entity in adults than it is in children. METHODS The records of all patients 18 years and older with the postoperative diagnosis of intussusception at the Massachusetts General Hospital during the years 1964 through 1993 were reviewed retrospectively. The 58 patients were divided into those with benign enteric, malignant enteric, benign colonic, and malignant colonic lesions associated with their intussusception. The diagnosis and treatment of each were reviewed. RESULTS In 30 years at the Massachusetts General Hospital, there are 58 cases of surgically proven adult intussusception. The patients' mean age was 54.4 years. Most patients presented with symptoms consistent with bowel obstruction. There were 44 enteric and 14 colonic intussusceptions. Ninety-three percent of the intussusceptions were associated with a pathologic lesion. Forty-eight percent of the enteric lesions were malignant and 52% were benign. Forty-three percent of the colonic lesions were malignant and 57% were benign. CONCLUSIONS Intussusception occurs rarely in adults. It presents with a variety of acute, intermittent, and chronic symptoms, thus making its preoperative diagnosis difficult. Computed tomography scanning proved to be the most useful diagnostic radiologic method. The diagnosis and treatment of adult intussusception are surgical. Surgical resection of the intussusception without reduction is the preferred treatment in adults, as almost half of both colonic and enteric intussusceptions are associated with malignancy.
Fat source and calcium level effects on finishing steer performance, digestion, and metabolism.

A 111-d finishing study evaluated animal growth and carcass characteristics using 138 steers (366 kg) in a randomized complete block design with a 2 x 3 factorial arrangement of treatments. The dietary treatments consisted of no supplemental fat or 3.5% tallow or soybean oil soapstock (SS) fed with . 6% and . 9% dietary Ca. Fat increased DMI (P less than .05) but interacted with Ca level (P less than .05) for gain/feed and ADG. All diets containing fat or . 9% Ca were converted more efficiently to gain than the . 6% Ca, no supplemental fat diet (P less than .05). The . 9% Ca interacted with fat source to decrease gain (P less than .05) and tended to decrease efficiency in the tallow diet but improved efficiency (P less than .05) and tended to improve gain in the no-fat diet. In the SS diet, . 9% Ca had no effect on ADG, DMI, or efficiency of gain. Fat addition increased backfat (P less than .10) and interacted with Ca on hot carcass weight, final weight, and dressing percentage (P less than .05). Feeding fat increased the proportion of 18:0 (P less than .02) and decreased the proportion of 16:1 fatty acids (P less than .06) in intermuscular fat. A replicated 3 x 3 Latin square design, using six Holstein steers (349 kg) fed three diets, with no supplemental fat or 3.5% SS or tallow with 1.0% Ca, was used to explore the effects of fat sources when fed with high Ca on digestion and metabolism. Ruminal fluid pH was higher (P less than .10) when steers were fed fat. Adding fat did not affect (P greater than .10) duodenal or ileal pH, VFA proportions or total concentration, or ruminal liquid volume or flow rate. Liquid retention time was shorter and liquid rate of passage was higher (P less than .05) with dietary fat addition. Adding fat did not affect site or extent of starch or DM digestion. There was net synthesis of 16:0, 18:0, and 18:1 fatty acids in the rumen. When steers were fed tallow, synthesis of 16:0 and 18:0 fatty acids in the rumen was lower (P less than .10) than when steers were fed SS. Feeding fat tended to decrease (P = .11) bacterial N flowing at the duodenum but did not affect nonbacterial N or total N. Fat addition seems to affect ruminal kinetics, and the effects may vary with fat source, particularly relative to fatty acid synthesis and digestion.
Absolute height-specific thresholds to identify elevated blood pressure in children

Objective: Identification of children with elevated blood pressure (BP) is difficult because of the multiple sex, age, and height-specific thresholds to define elevated BP. We propose a simple set of absolute height-specific BP thresholds and evaluate their performance to identify children with elevated BP in two different populations. Methods: Using the 95th sex, age, and relative-height BP US thresholds to define elevated BP in children (standard criteria), we derived a set of (non sex- and non age-specific) absolute height-specific BP thresholds for 11 height categories by 10 cm increments. Using data from large school-based surveys conducted in Switzerland (N = 5207; 2621 boys, 2586 girls; age range: 10.1–14.9 years) and in the Seychelles (N = 25 759; 13 048 boys, 12 711 girls; age range: 4.4–18.8 years), we evaluated the performance of these height-specific thresholds to identify children with elevated BP. We also derived sex-specific absolute height-specific BP thresholds and compared their performance. Results: In the Swiss and the Seychelles surveys, the prevalence of elevated BP (standard criteria) was 11.4 and 9.1%, respectively. The height-specific thresholds to identify elevated BP had a sensitivity of 80 and 84%, a specificity of 99 and 99%, a positive predictive value of 92 and 91%, and a negative predictive value of 97 and 98%, respectively. Performance of sex-specific absolute height-specific BP thresholds was similar. Conclusion: A simple table of height-specific BP thresholds allowed identifying children with elevated BP with high sensitivity and excellent specificity.
Determination and Analysis of Toluene Diisocyanate Metabolites in Mice

This paper is to to determine metabolites of toluene diisocyanate (TDI) in mice and deduced the pathway for toluene diisocyanate metabolism in the organism. Toluene diisocyanate was administered to mice for 2 weeks, samples of blood, feces, and urine were taken, and the structure of the toluene diisocyanate metabolites was determined using gas chromatography-mass spectrometry (GC-MS). Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column (30 mm times 0.25 mm times 0.25 mum); initial column temperature: 90degC, which was maintained for 30 min, after which the temperature was increased at a rate of 40degC/min to 280degC, which temperature was then maintained for 5.25 min; temperature for the vaporizing chamber: 250degC; carrier gas: helium flowing at 1.0 mL/min. Conditions for chromatography of TDI metabolites in the organism: 94% methyl, 4% ethenyl-bonded-phase fused-silica capillary column (30 + 2 mX 0.25 + 0.02 mm); initial column temperature: 30degC, which was maintained for 5 min, after which the temperature was increased at a rate of 80degC/min to 280degC, which temperature was then maintained for 5 min; temperature for the vaporizing chamber: 250degC; carrier gas: helium flowing at 1.0 mL/min. Conditions for mass spectrometry: EI for ionization; 70 eV for ionization energy; 280degC for connecting tube temperature; 35-350 amu for range of scanning; and 1.0 muL for sample size. The results showed 2,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene, which is completely the same in male and female animals. Under the conditions selected for GC-MS, TDI metabolites in the organism can be isolated. Endogenous impurities in the blood, feces, or urine of mice do not interfere with the measurements.
Empathy, Sense of Power, and Personality: Do They Change During Pediatric Residency?

Objectives Empathy is a critical competency in medicine. Prior studies demonstrate a longitudinal decrease in empathy during residency; however, they have not included pediatric residents. The relations among the expression of empathy, sense of power (ability to influence other’s behavior), and personality traits in residents also have not been addressed. Lastly, there are no data on how residents compare with the general nonmedical population in their expression of empathy. The purposes of our study were to assess whether empathy, sense of power, and personality type were statistically correlated; if resident empathy declines over time; and how resident empathy compares with that of nonmedical peers. Methods In 2010, a cohort of individuals entering pediatric residency were given three validated survey instruments at the beginning of their first and third years of training to explore longitudinal changes in empathy, sense of power, and major personality traits. Results We found no decrease in resident empathy in 2 years of pediatric training, no changes in their sense of power, and no statistically significant correlation between empathetic tendencies and sense of power. When compared with the general nonmedical population, pediatric residents rated themselves higher in empathy. As expected, the two components of empathy (empathic concern and perspective taking) were moderately correlated. Of the major personality traits, only agreeableness showed significant correlation with empathy. Conclusions Pediatric resident empathy did not decrease longitudinally, unlike studies in other residents. There was no inverse relation between self-perceptions of sense of power and empathy as is present in the business literature. Finally, pediatric resident empathy was significantly higher when compared with a general nonmedical population.
Distinct frequency preferences of different types of rat hippocampal neurones in response to oscillatory input currents

1 Coherent network oscillations in several distinct frequency bands are seen in the hippocampus of behaving animals. To investigate how different neuronal types within this network respond to oscillatory inputs we made whole‐cell current clamp recordings from three different types of neurones in the CA1 region of rat hippocampal slices: pyramidal cells, fast‐spiking interneurones and horizontal interneurones, and recorded their response to sinusoidal inputs at physiologically relevant frequencies (1‐100 Hz). 2 Pyramidal neurones showed firing preference to inputs at theta frequencies (range 2‐7 Hz; n= 30). They showed subthreshold resonance in the same frequency range (2‐7 Hz; mean 4.1 ± 0.4 Hz; n= 19). 2 Interneurones differed in their firing properties. Horizontal interneurones in the stratum oriens showed firing preference to inputs at theta frequencies (range 1.5‐10 Hz; n= 10). These interneurones also showed resonance at low frequencies (range 1‐5 Hz; mean 2.4 ± 0.5 Hz; n= 7). In contrast, fast‐spiking interneurones with cell bodies in the pyramidal cell layer fired preferentially at input frequencies in the gamma band (range 30‐50 Hz; n= 10/12). These interneurones showed resonance at beta‐gamma frequencies (10‐50 Hz; mean 26 ± 5 Hz; n= 7/8). 3 Thus, in the hippocampus, different types of neurones have distinct frequency preferences. Therefore, in the CA1 layer of the hippocampal network, a compound oscillatory input may be segregated into distinct frequency components which are processed locally by distinct types of neurones.