Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression and analysis of two gyrB genes from the novobiocin producer, Streptomyces sphaeroides

A novobiocin producer, Streptomyces sphaeroides, contains two genes, designated gyrBs and gyrBR, that encode novobiocin‐sensitive and ‐resistant DNA gyrase B proteins, respectively. The cloning of gyrBR was reported earlier; here, we describe the cloning of gyrBS. Both genes have been sequenced (the deduced products of gyrBs and gyrBR have Mr values of 87.6K and 86.5 K, respectively) and their transcripts have been mapped. Downstream of gyrBS, and co‐transcribed with it, is the sole gyrA gene (encoding DNA gyrase A protein). By constructing hybrid gyrBS genes, using fragments of gyrBS and gyrBR, a specific portion of the N‐terminal domain of the gyrase B protein (corresponding to amino acid residues 134–256 of Escherichia coli gyrase B) has been implicated in the binding of novobiocin.
Sonographic changes of hydatid cyst of the liver after treatment with mebendazole and albendazole.

Hydatid disease is a tape-worm infection caused by either Echinococcus granulosus or Echinococcus multilocularis. The disease is endemic in many countries throughout the world. In man, the liver is the most common organ involved by this parasite because the route of entry of the larvae is the portal venous system (Sherlock, 1981). The radiological appearances of hydatid disease have been described previously, including ultrasound and computed tomography (Itzchak et al, 1980; Gharbi et al, 1981; Niron & Ozer, 1981; Hadidi, 1982; Choliz et al, 1982; Beggs, 1983). Not many reports have mentioned the changes after medical treatment (Bekhti et al, 1977; Morris et al, 1983, 1984). We report the serial sonographic changes in a patient with a hepatic hydatid cyst who has been treated with mebendazole and albendazole during a five-year period.
Interferon-alpha in lupus psychosis.

OBJECTIVE Since the level of interferon-alpha (IFN alpha) is increased in the sera of patients with active systemic lupus erythematosus (SLE) and is detectable in the cerebrospinal fluid (CSF) of some SLE patients with neuropsychiatric manifestations, we investigated the contribution of IFN alpha to the pathogenesis of the neuropsychiatric manifestations of SLE. METHODS IFN alpha levels were quantitated by radio-immunoassay in CSF and serum samples from 17 SLE patients with neuropsychiatric manifestations and 28 patients with SLE alone or SLE and other neurologic disorders. RESULTS Levels of IFN alpha were increased in the CSF of 5 of 6 patients with lupus psychosis, and in 4 of these 5 patients, the levels in CSF were higher than those in serum. IFN alpha levels decreased when the manifestation of lupus psychosis subsided. In contrast, IFN alpha levels in CSF samples from patients with seizures alone were not increased. One patient with lupus psychosis died of complications of generalized seizures resulting from the SLE. At autopsy, we investigated whether IFN alpha protein or messenger RNA was detectable in the subject's brain. IFN alpha protein was immunohistochemically demonstrated in the neurons and in the microglia (focal accumulation), features not present in the brain tissues of subjects who died of other diseases. CONCLUSION These findings support the hypothesis that IFN alpha, possibly synthesized in the brain, is the cause of the manifestation of psychosis in patients with SLE.
Baroclinic and Barotropic Annular Variability in the Northern Hemisphere

AbstractLarge-scale variability in the Northern Hemisphere (NH) circulation can be viewed in the context of three primary types of structures: 1) teleconnection patterns, 2) a barotropic annular mode, and 3) a baroclinic annular mode. The barotropic annular mode corresponds to the northern annular mode (NAM) and has been examined extensively in previous research. Here the authors examine the spatial structure and time-dependent behavior of the NH baroclinic annular mode (NBAM). The NAM and NBAM have very different signatures in large-scale NH climate variability. The NAM emerges as the leading principal component (PC) time series of the zonal-mean kinetic energy. It dominates the variance in the wave fluxes of momentum, projects weakly onto the eddy kinetic energy and wave fluxes of heat, and can be modeled as Gaussian red noise with a time scale of ~10 days. In contrast, the NBAM emerges as the leading PC time series of the eddy kinetic energy. It is most clearly identified when the planetary-scale waves .
Intravenous Immunoglobulin Doesn’t Decrease Mortality for Suspected or Proven Sepsis in the Neonate

163 Journal of Clinical Neonatology | Vol. 2 | Issue 4 | October-December 2013 the benefi t of NIPPV in preterm infants with RDS, with a signifi cant reduction in the need for intubation and invasive mechanical ventilation within the fi rst 72 h of life. [5] Th is is the largest international study to date compared two current strategies for noninvasive respiratory support its showed an unexpected result that nasal intermittent positive-pressure ventilation does not improve BPD and the risk for reintubation. NCPAP has become the standard of care as initial respiratory support for preterm infants with RDS in many centers, its simple and widely used, but NCPAP failure is exciting. BPD is a complex disease and the need for mechanical ventilation especially early in life, is a signifi cant risk factor for its development. Th is new fi nding put the clinicians on the dilemma of deciding on neonatal ventilation options aiming to prevent lung damage in extreme preterm infants.
Overview of the LADCO winter nitrate study: hourly ammonia, nitric acid and PM 2.5 composition at an urban and rural site pair during PM 2.5 episodes in the US Great Lakes region

Abstract. An overview of the LADCO (Lake Michigan Air Directors Consortium) Winter Nitrate Study (WNS) is presented. Sampling was conducted at ground level at an urban-rural pair of sites during January–March 2009 in eastern Wisconsin, toward the western edge of the US Great Lakes region. Areas surrounding these sites experience multiday episodes of wintertime PM2.5 pollution characterized by high fractions of ammonium nitrate in PM, low wind speeds, and air mass stagnation. Hourly surface monitoring of inorganic gases and aerosols supplemented long-term 24-h aerosol chemistry monitoring at these locations. The urban site (Milwaukee, WI) experienced 13 PM2.5 episodes, defined as periods where the seven-hour moving average PM2.5 concentration exceeded 27 μg m−3 for at least four consecutive hours. The rural site experienced seven episodes by the same metric, and all rural episodes coincided with urban episodes. Episodes were characterized by low pressure systems, shallow/stable boundary layer, light winds, and increased temperature and relative humidity relative to climatological mean conditions. They often occurred in the presence of regional snow cover at temperatures near freezing, when snow melt and sublimation could generate fog and strengthen the boundary layer inversion. Substantial contribution to nitrate production from nighttime chemistry of ozone and NO2 to N2O5 and nitric acid is likely and requires further investigation. Pollutant-specific urban excess during episode and non-episode conditions is presented. The largest remaining uncertainties in the conceptual model of the wintertime episodes are the variability from episode-to-episode in ammonia emissions, the balance of daytime and nighttime nitrate production, the relationship between ammonia controls, NOx controls and ammonium nitrate reductions, and the extent to which snow and fog are causal (either through meteorological or chemical processes) rather than just correlated with episodes because of similar synoptic meteorology.
Incidence, predictors, and prognostic value of intramyocardial hemorrhage lesions in ST elevation myocardial infarction

Background: Intra myocardial hemorrhage lesions (IMH) are underdiagnosed complication of ST elevation myocardial infarction (STEMI). We sought to determine the incidence, predictors and the prognostic value of IMH in STEMI using cardiac MR imaging (CMR) techniques. Methods: We screened for inclusion consecutive patients with STEMI treated by percutaneous coronary intervention (PCI) within the first 12 hr of evolution. IMH lesions were identified on T2‐weighted sequences on CMR between days 4 and 8 after PCI. Adverse cardiac events were defined as a composite of death + severe ventricular arrhythmias + acute coronary syndrome + acute heart failure. Results: N = 114 patients were included and n = 11 patients (10%) presented IMH lesions. Patients with IMH lesions had a larger myocardial infarction extent (25.6 ± 1.8 vs. 13.5 ± 1.0 % LV mass, P < 0.01), microvascular obstructive lesions extent (4.6 ± 1.0 vs. 1.3 ± 0.3% LV mass, P < 0.01) and lower LV ejection fraction (40.7 ± 2.3% vs. 50.7 ± 1.3%, P < 0.01). The value of glycemia at admission was an independent predictor of IMH development (Odd ratio 1.8 [1.1–2.8] per mmol l−1, P = 0.01). The incidence of adverse cardiac events was higher in the IMH group than in the non‐IMH group during the first year following STEMI (P = 0.01, log‐rank analysis). Cox regression analysis identified the presence of IMH lesions as an independent predictor of adverse clinical outcome (Hazard Ratio = 2.8 [1.2–6.8], P = 0.02). Conclusion: Our study indicates that IMH is a rare but severe finding in STEMI, associated with a larger myocardial infarction and a worse clinical outcome. Per‐PCI glycemia might influence IMH development. © 2011 Wiley Periodicals, Inc.
The International Society for Sexual Medicine's Process of Care for the Assessment and Management of Testosterone Deficiency in Adult Men.

INTRODUCTION In 2014, the International Society for Sexual Medicine (ISSM) convened a panel of experts to develop an evidence-based process of care for the diagnosis and management of testosterone deficiency (TD) in adult men. The panel considered the definition, epidemiology, etiology, physiologic effects, diagnosis, assessment and treatment of TD. It also considered the treatment of TD in special populations and commented on contemporary controversies about testosterone replacement therapy, cardiovascular risk and prostate cancer. AIM The aim was to develop clearly worded, practical, evidenced-based recommendations for the diagnosis and treatment of diagnosis and management of TD for clinicians without expertise in endocrinology, such as physicians in family medicine and general urology practice. METHOD A comprehensive literature review was performed, followed by a structured, 3-day panel meeting and 6-month panel consultation process using electronic communication. The final guideline was compiled from reports by individual panel members on areas reflecting their special expertise, and then agreed by all through an iterative process. RESULTS This article contains the report of the ISSM TD Process of Care Committee. It offers a definition of TD and recommendations for assessment and treatment in different populations. Finally, best practice treatment recommendations are presented to guide clinicians, both familiar and unfamiliar with TD. CONCLUSION Development of a process of care is an evolutionary process that continually reviews data and incorporates the best new research. We expect that ongoing research will lead to new insights into the pathophysiology of TD, as well as new, efficacious and safe treatments. We recommend that this process of care be reevaluated and updated by the ISSM in 4 years.
Coffee Consumption and the Incidence of Colorectal Cancer in Women

Background. Higher coffee consumption has been associated with decreased incidence of colorectal cancer. Our objective was to examine the relationship of coffee intake to colorectal cancer incidence in a large observational cohort of postmenopausal US women. Methods. Data were collected for the Women's Health Initiative Observational Study providing a follow-up period of 12.9 years. The mean age of our sample (N = 83,778 women) was 63.5 years. Daily coffee intake was grouped into 3 categories: None, moderate (>0–<4 cups), and high (4+ cups). Proportional hazards modeling was used to evaluate the relationship between coffee intake and colorectal cancer. Results. There were 1,282 (1.53%) new cases of colorectal cancer during follow-up. Compared to nondrinkers, moderate and high coffee drinkers had an increased incidence of colorectal cancer in multivariate analysis (HR 1.15, 1.02–1.29; HR 1.14, 0.93–1.38). Moderate drip brew coffee intake (HR 1.20, 1.05–1.36) and high nondrip brew coffee intake (HR 1.43, 1.01–2.02) were associated with increased odds. Conclusion. Our results suggesting increased incidence of colorectal cancer associated with higher coffee consumption contradict recent meta-analyses but agree with a number of other studies showing that coffee increases risk or has no effect. Brew method results are novel and warrant further research.
Transcriptional changes in Teladorsagia circumcincta upon encountering host tissue of differing immune status

SUMMARY The aim of this study was to elucidate transcriptional changes in the parasitic nematode Teladorsagia circumcincta upon encountering either naïve or immune ovine hosts. Pools of 100 000 exsheathed 3rd- stage T. circumcincta larvae were exposed in vitro to either an immune or naïve ovine abomasal environment, RNA was extracted from the larvae and sequenced using the Roche 454 platform. Each sample produced approximately 82 000 reads that assembled to give approximately 5500 Isotigs (contigs). The two sequence datasets were clustered together to give a total of 6969 clusters of which 18 were differentially expressed (P<0·001) between the two groups. Clusters with a predominance of reads in larvae exposed to the immune abomasal environment encoded homologues of peptidyl-glycine alpha-amidating monooxygenase, heat shock-protein 16-2 and IDA-1, a tyrosine phosphatase-like receptor protein. Clusters with a predominance of reads in the naïve environment encoded homologues of cytochrome b, EGg Laying defective family member 21 and NADH dehydrogenase subunit 5. Gene ontology analyses indicated that larvae exposed to the immune environment showed an increase in expression of genes involved in ‘carbon utilization’, ‘response to stimulus’ and ‘developmental process’. These data suggest that T. circumcincta modulates gene expression in response to the immune status of the host.
Sign- versus goal-tracking: effects of conditioned-stimulus-to-unconditioned-stimulus distance.

Three pigeons were exposed sequentially across experimental phases to five different distances between the conditioned stimulus and the site of the unconditioned stimulus in a sign-/goal-tracking procedure. A computer-controlled tracking system provided a continuous record of the bird's position by continuously monitoring the location of the bird's head in three-dimensional space. It was found that birds sign-tracked (i.e., approached the conditioned stimulus) when the conditioned stimulus was closest to the site of the unconditioned stimulus, goal-tracked (i.e., approached the site of the unconditioned stimulus in the presence of the conditioned stimulus) when the conditioned stimulus was farthest from the site of the unconditioned stimulus, and engaged in both sign- and goal-tracking (or something intermediate) at intermediate conditioned-stimulus-to-unconditioned-stimulus distances. When both sign- and goal-tracking occurred, the former tended to occur in the first half and the latter in the second half of the interval in which the conditioned stimulus was present. The results suggest (a) whether sign- or goal-tracking (or both) occurs is a function of the distance of the conditioned stimulus from the site of the unconditioned stimulus, (b) the fact that pigeons but not rats have been found to sign-track consistently throughout the duration of the conditioned stimulus may be due to quantitatively rather than qualitatively different effects of conditioned-stimulus-to-unconditioned-stimulus distance across species (i.e., a "short" conditioned-stimulus-to-unconditioned-stimulus distance for a pigeon may be a "long" one for a rat), and (c) sign- and goal-tracking may be competing behavioral tendencies that can (e.g., at intermediate conditioned-stimulus-to-unconditioned-stimulus distances) cancel each other out. The findings lend support to theories that specify an interaction between phylogenetic and reinforcement variables in determining whether sign- or goal-tracking will occur in any given experimental preparation.
Responses to Controlled Diesel Vapor Exposure Among Chemically Sensitive Gulf War Veterans

Objective: A significant proportion of Gulf War veterans (GWVs) report chemical sensitivity, fatigue, and unexplained symptoms resulting in ongoing disability. GWVs frequently recall an association between diesel and petrochemical fume exposure and symptoms during service. The purpose of the present study among GWVs was to evaluate the immediate health effects of acute exposure to chemicals (diesel vapors with acetaldehyde) with and without stress. Methods: In a single, controlled exposure to 5 parts per million (ppm) diesel vapors, symptoms, odor ratings, neurobehavioral performance, and psychophysiologic responses of 12 ill GWVs (GWV-I) were compared with 19 age- and gender-matched healthy GWVs (GWV-H). Results: Relative to baseline and to GWV-H, GWV-I reported significantly increased symptoms such as disorientation and dizziness and displayed significantly reduced end-tidal CO2 just after the onset of exposure. As exposure increased over time, GWV-I relative to GWV-H reported significantly increased symptoms of respiratory discomfort and general malaise. GWV-I were also physiologically hyporeactive in response to behavioral tasks administered during but not before exposure. Conclusions: Current symptoms among GWV-I may be exacerbated by ongoing environmental chemical exposures reminiscent of the Gulf War. Both psychologic and physiologic mechanisms contribute to current symptomatic responses of GWV-I.
Do Psychological Well-Being characteristics vary with Birth Order?

The effect of Birth Order on Personality has remained a debated research topic with studies favouring and not favouring the possible impacts. While there has been substantial research separately on Birth Order and Psychological Well-Being (PWB) scarcely can we find studies relating Birth Orders with various sub-scales of PWB.The present study was thus conducted amongst students of Mysore (n=273) to investigate the whether the Birth Order characteristics vary with the sub-scales of Ryff’s PWB scale. Significant differences were found among all Birth Orders. Research implications and significance of the study conclude the article.
The Human Cytomegalovirus Major Immediate-Early Distal Enhancer Region Is Required for Efficient Viral Replication and Immediate-Early Gene Expression

ABSTRACT The human cytomegalovirus (HCMV) major immediate-early (MIE) genes, encoding IE1 p72 and IE2 p86, are activated by a complex enhancer region (base positions -65 to -550) that operates in a cell type- and differentiation-dependent manner. The expression of MIE genes is required for HCMV replication. Previous studies analyzing functions of MIE promoter-enhancer segments suggest that the distal enhancer region variably modifies MIE promoter activity, depending on cell type, stimuli, or state of differentiation. To further understand the mechanism by which the MIE promoter is regulated, we constructed and analyzed several different recombinant HCMVs that lack the distal enhancer region (-300 to -582, -640, or -1108). In human fibroblasts, the HCMVs without the distal enhancer replicate normally at high multiplicity of infection (MOI) but replicate poorly at low MOI in comparison to wild-type virus (WT) or HCMVs that lack the neighboring upstream unique region and modulator (-582 or -640 to -1108). The growth aberrancy was normalized after restoring the distal enhancer in a virus lacking this region. For HCMVs without a distal enhancer, the impairment in replication at low MOI corresponds to a deficiency in production of MIE RNAs compared to WT or virus lacking the unique region and modulator. An underproduction of viral US3 RNA was also evident at low MOI. Whether lower production of IE1 p72 and IE2 p86 causes a reduction in expression of the immediate-early (IE) class US3 gene remains to be determined. We conclude that the MIE distal enhancer region possesses a mechanism for augmenting viral IE gene expression and genome replication at low MOI, but this regulatory function is unnecessary at high MOI.
Anterior shift in gene expression precedes anteriormost digit formation in amniote limbs

In tetrapod limbs, an anteriormost digit has common traits of small, short and less‐phalange morphology. In this study, we focused on three genes, Mkp3, Sef and Tsukushi (TSK), which have anterior‐specific or anterior‐prominent expression patterns in the developing limb bud at the autopod‐forming stage. The anterior expression is not fixed in the period of limb development, but the expression domains of Mkp3, Sef and TSK change considerably from the distal domain to the anterior domain. This change in expression domains, anterior shift, of these genes involves maintenance of gene expression in the anterior side and downregulation in the posterior side. Manipulated overdose of fibroblast growth factor (FGF) in the presumptive digit 2 region of chick forelimb bud results in elongation of cartilage elements of digit 2, suggesting that attenuated FGF signaling, which Mkp3, Sef, and TSK negatively regulate, provides digit 2‐specific traits of morphology. The anterior expression of Mkp3 and Sef but not TSK is conserved also in limb buds of the mouse and gecko, and the anterior shift of these genes, accumulation of their transcripts in the anterior side and appropriate regulation of strength of FGF signaling may control species‐specific morphology of the anteriormost digit.
Detection of varicella‐zoster virus by dot‐blot hybridization using a molecularly cloned viral DNA probe

Varicella‐Zoster Virus (VZV) infection can be definitively diagnosed by isolation of virus in cell culture, a process that usually takes 7–14 days. In order to facilitate the more rapid detection of this virus, we developed a technique for hybridization of DNA from clinical specimens using an in vitro‐labeled mixture of cloned fragments of VZV DNA as a probe. The assay can be completed in 36–48 hr and can be successfully carried out in the range of 10 pg to 10 ng of viral DNA. In analyses of 38 specimens from patients with a clinical diagnosis of VZV infection, the results of viral isolation and this assay were highly concordant. The sensitivity of standard cell culture for detection of VZV was 58%, whereas the sensitivity of the assay was 76%, not significantly different (P = 0.14). The specificity of cell culture was 100%, whereas that of the assay was 94% (P = 0.49). The technique appears to be sensitive, specific, and useful for analyses of tissues and body fluids.