Miracle-dz/UniFAR-FTUs huggingface dataset:
Title High expression of trimethylated histone H 3 at lysine 27 predicts betterprognosis in non-small cell lung cancer

Epigenetic parameters such as DNA methylation and histone modifications play pivotal roles in carcinogenesis. Global histone modification patterns have been implicated as possible predictors of cancer recurrence and prognoses in a great variety of tumor entities. Our study was designed to evaluate the association among trimethylated histone H3 at lysine 27 (H3K27me3), clinicopathological variables and outcome in early-stage non-small cell lung cancer (NSCLC). The expression of H3K27me3 and its methyl transferase, enhancer of zeste homolog 2 (EZH2) together with proliferating cell nuclear antigen (PCNA) were evaluated by immunohistochemistry in normal lung tissue (n=5) and resected NSCLC patients (n=42). In addition, the specificity of antibody for H3K27me3 was tested by western blot analysis. The optimal cut-off point of H3K27me3 expression for prognosis was determined by the X-tile program. The prognostic significance was determined by means of KaplanMeier survival estimates and log-rank tests. As a result, enhanced trimethylation of H3K27me3 was correlated with longer overall survival (OS) and better prognosis (P<0.05). Moreover, both univariate and multivariate analyses indicated that H3K27me3 level was a significant and independent predictor of better survival (hazard ratio, 0.187; 95% confidence interval, 0.066-0.531, P=0.002). Furthermore, H3K27me3 expression was positively correlated with DNA methylation level at CCGG sites while reversely related to EZH2 expression (P<0.05). In conclusion, H3K27me3 level defines unrecognized subgroups of NSCLC patients with distinct epigenetic phenotype and clinical outcome, and can probably be used as a novel predictor for better prognosis in NSCLC patients.
Drivers of plant invasion vary globally: evidence from pine invasions within six ecoregions

Aim To determine biotic and abiotic controls on pine invasion globally within six ecoregions that include both introduced and native ranges. Locations Rio Negro province, Argentina; Aysen and Araucania regions, Chile; South Island (two ecoregions), New Zealand; Greater Yellowstone ecosystem, USA. Methods We quantified tree abundance and size across invasion fronts of the widespread invasive tree species Pinus contorta at each of the nine sites, encompassing both the native and introduced range. We also determined the relative importance of propagule pressure, abiotic characteristics and biotic factors for invasion success. Finally, key plant population metrics such as individual tree growth rates and reproductive effort were compared between native and introduced ranges. Results Pinus contorta density decreased with increasing distance from source population in all cases, but the importance and shape of this relationship differed among sites due, primarily to biotic factors. For example, areas dominated by native southern beech forest (Fuscospora cliffortioides or Nothofagus spp.) were not invaded, and this biotic resistance was not overcome by high propagule pressure. In contrast, shrublands were more highly invaded than grasslands, contradicting previous generalizations about pine invasions. Pinus contorta growth was faster, age to maturity was earlier and reproductive effort was higher in the introduced ranges compared with the native range, suggesting a demographic shift towards more rapid population growth in introduced regions. Climatic differences between the ranges may explain, at least in part, the observed pattern. Main conclusions We demonstrate that although biological invasions are driven by propagule pressure across different ecoregions, these processes interact strongly with biotic factors. Intriguingly, our results suggest that propagule pressure may become less important than biotic interactions as invasions proceed. Multi-region studies including both the native and introduced ranges provide unparalleled opportunities for understanding how these interactions change among regions as invasions proceed.
Intestinal Barrier Dysfunction in a Randomized Trial of a Specific Probiotic Composition in Acute Pancreatitis

Objectives:To determine the relation between intestinal barrier dysfunction, bacterial translocation, and clinical outcome in patients with predicted severe acute pancreatitis and the influence of probiotics on these processes. Summary of Background data:Randomized, placebo-controlled, multicenter trial on probiotic prophylaxis (Ecologic 641) in patients with predicted severe acute pancreatitis (PROPATRIA). Methods:Excretion of intestinal fatty acid binding protein (IFABP, a parameter for enterocyte damage), recovery of polyethylene glycols (PEGs, a parameter for intestinal permeability), and excretion of nitric oxide (NOx, a parameter for bacterial translocation) were assessed in urine of 141 patients collected 24 to 48 h after start of probiotic or placebo treatment and 7 days thereafter. Results:IFABP concentrations in the first 72 hours were higher in patients who developed bacteremia (P = 0.03), infected necrosis (P = 0.01), and organ failure (P = 0.008). PEG recovery was higher in patients who developed bacteremia (PEG 4000, P = 0.001), organ failure (PEG 4000, P < 0.0001), or died (PEG 4000, P = 0.009). Probiotic prophylaxis was associated with an increase in IFABP (median 362 vs. 199 pg/mL; P = 0.02), most evidently in patients with organ failure (P = 0.001), and did not influence intestinal permeability. Overall, probiotics decreased NOx (P = 0.05) but, in patients with organ failure, increased NOx (P = 0.001). Conclusions:Bacteremia, infected necrosis, organ failure, and mortality were all associated with intestinal barrier dysfunction early in the course of acute pancreatitis. Overall, prophylaxis with this specific combination of probiotic strains reduced bacterial translocation, but was associated with increased bacterial translocation and enterocyte damage in patients with organ failure.
Multicenter phase II trial of S-1 plus cisplatin in patients with untreated advanced gastric or gastroesophageal junction adenocarcinoma.

PURPOSE S-1 plus cisplatin is considered highly active in Japanese gastric cancer patients. We conducted a phase II multi-institutional trial, in the West, in patients with untreated advanced gastric or gastroesophageal junction adenocarcinoma to evaluate activity and safety of this combination. METHODS Patients received cisplatin intravenously at 75 mg/m2 on day 1 and S-1 orally at 25 mg/m2/dose bid (50 mg/m2/d) on days 1 to 21, repeated every 28 days. Patients with histologic proof of gastric or gastroesophageal junction adenocarcinoma with a Karnofsky performance status (KPS) of > or = 70% and near-normal organ function were eligible. All patients provided a written informed consent. To observe a 45% confirmed overall response rate (ORR), 41 assessable patients were needed. RESULTS All 47 patients were assessed for safety and survival, and 41 patients were assessed for ORR. The median age was 56 years and median KPS was 80%. The median number of chemotherapy cycles was four. The confirmed ORR was 51% (95% CI, 35% to 67%) and it was 49% by an independent review. At the 6-month interval, 71% of patients were alive, with a median survival time of 10.9 months. Frequent grade 3 or 4 toxicities included fatigue (26%), neutropenia (26%), vomiting (17%), diarrhea (15%), and nausea (15%); however, stomatitis (2%) and febrile neutropenia (2%) were uncommon. There was one (2%) treatment-related death. CONCLUSION S-1 plus cisplatin is active against gastric cancer and has a favorable toxicity profile. A global phase III study of S-1 plus cisplatin versus fluorouracil plus cisplatin currently is accruing patients.
Data Analysis for Continuous EEG Monitoring in the ICU: Seeing the Forest and the Trees

Continuous EEG monitoring (CEEG) is a powerful tool for evaluating cerebral function in obtunded and comatose critically ill patients. The ongoing analysis of CEEG data is a major task because of the volume of data generated during monitoring and the need for near real-time interpretation of a patient's EEG patterns. Advances in digital EEG data acquisition, computer processing, data transmission, and data display have made CEEG monitoring in the intensive care unit technically feasible. A variety of quantitative EEG tools such as Fourier analysis and amplitude-integrated EEG, and other methods of data analysis such as computerized seizure detection, increasingly allow for focused review of EEG epochs of potential interest. These tools reduce the tremendous time burdens that accompany analysis of the complete CEEG data stream, and allow bedside personnel and nonexpert staff to potentially recognize significant EEG changes in a timely fashion. This article uses literature review and clinical case examples to illustrate techniques for the display and analysis of intensive care unit CEEG recordings. Areas requiring further research and development are discussed.
The shift towards multi-disciplinarity in information science

This article analyzes the collaboration trends, authorship and keywords of all research articles published in the Journal of American Society for Information Science and Technology (JASIST). Comparing the articles between two 10-year periods, namely, 1988–1997 and 1998–2007, the three-fold objectives are to analyze the shifts in (a) authors’ collaboration trends (b) top authors, their affiliations as well as the pattern of coauthorship among them, and (c) top keywords and the subdisciplines from which they emerge. The findings reveal a distinct tendency towards collaboration among authors, with external collaborations becoming more prevalent. Top authors have grown in diversity from those being affiliated predominantly with library/information-related departments to include those from information systems management, information technology, businesss, and the humanities. Amid heterogeneous clusters of collaboration among top authors, strongly connected crossdisciplinary coauthor pairs have become more prevalent. Correspondingly, the distribution of top keywords’occurrences that leans heavily on core information science has shifted towards other subdisciplines such as information technology and sociobehavioral science.
Costus speciosus (Keukand): A review

Costus speciosus Koen. (Keu, Crape ginger), an Indian ornamental plant, has long been medicinally used in traditional systems of medicine. The plant has been found to possess diverse number of pharmacological activities. The present article gives an account of updated information on its phytochemical and pharmacological properties. The review reveals that wide numbers of phytochemical constituents have been isolated from the plant which possesses activities like be bitter, astringent, aphrodisiac, purgative, anthelmintic, depurative, febrifuge and expectorant. Rhizomes are given in pneumonia, rheumatism, dropsy, urinary diseases, jaundice while leaves are given in mental disorders. Bruised leaves are applied in fever; decoction of stem is used in fever and dysentery. Various other activities are also reported like anti-inflammatory, antiarthritic effect and antifungal activities. The plant is also used in gout rheumatism and bronchial asthma. Rhizomes of plant exhibit cardiotonic, hydrochloretic, diuretic and CNS depressant activities. These reports are very encouraging and indicate that the plant should be studied more extensively for its therapeutic effects.
Commercial WWW site appeal: how does it affect online food and drink consumers' purchasing behavior?

This paper reports the results of a recent online survey of consumer attitudes toward selected US online storefronts marketing barbecue sauce, cheese, olive oil, and potato chips as well as international companies marketing an assortment of specialty food products. It describes the relationship between consumer attitudes toward a commercial WWW site and likelihood of purchase as well as demographic factors which are related to online purchasing behavior of food and drink products.
International Spinal Injection Society guidelines for the performance of spinal injection procedures. Part 1: Zygapophysial joint blocks.

THORACIC ZYGAPOPHYSIAL JOINT BLOCKS I. Historical background Thoracic zygapophysial joint blocks are a relatively recent development. The literature is meagre, amounting to an abstract describing a case series,8 a paper describing technique,6 and a study in normal volunteers.5 Consequently, the prevalence of thoracic zygapophysial joint pain is not known, and the utility of thoracic zygapophysial joint blocks is, as yet, only conjectural.
Land Use Classification: A Surface Energy Balance and Vegetation Index Application to Map and Monitor Irrigated Lands

Irrigated agriculture consumes the largest share of available fresh water, and awareness of the spatial distribution and application rates is paramount to a functional and sustainable communal consumptive water use. This remote sensing study leverages surface energy balance fluxes and vegetation indices to classify and map the spatial distribution of irrigated and non-irrigated croplands. The purpose is to introduce a classification scheme applicable across a wide variation in regional climate and inter-growing seasonal precipitation. The rationale for climate and inter-growing seasonal adaptability is founded in the derivation and calibration of the scheme based on the wettest growing season. Therefore, the scheme becomes a more efficient classifier during normal and dry growing seasons. Using empirical distribution functions, two indices are derived from evapotranspiration fluxes and vegetation indices to contrast and classify irrigated croplands from non-irrigated. The synergy of the two indices increases the classification proficiency by adding another classifying layer which re-characterizes misclassified croplands by the base index. The scheme was applied to a region with wide climate variation and to multiple years of growing seasons. The results presented, in cross validation with groundtruth, show an accurate and consistent approach to classify irrigation with overall accuracy of 92.1%, applicable from humid to semi-arid climate, and from dry to normal and wet growing seasons.
Effect of amplitude modulation coherence for masked speech signals filtered into narrow bands.

Introduction of masker amplitude modulation (AM) can improve signal detection in a number of paradigms. In some cases this advantage depends on the coherence of modulation across a relatively wide frequency range. In the experiments described below, observers were asked to identify masked spondee words produced by a single male talker. The target spondees and masking noise were filtered into nine narrow bands, and the coherence of AM of either the speech signal or noise masker was manipulated. Inherent modulation of the masker bands was manipulated via assignment of real and imaginary values to the associated components of each band in the frequency domain, and AM of speech bands was achieved via multiplication with envelopes extracted from these maskers. Responses were based on two alternatives, four alternatives, or open response sets. The effect of masker AM coherence was highly dependent upon the size of the response set: coherent AM was associated with better thresholds in a two-alternative response set, but poorer thresholds in an open response set. Results with AM speech did not depend critically upon the across-frequency temporal synchrony of AM imposed on the speech material.
Isolation of a New Antimicrobial/Antitumor Plant Peptide: Biotechnology Prospects for its Use in Cancer and Infectious Diseases Therapies

The immune system of multi-cellular organisms comprises a vast arsenal of mechanisms to protect the host from the continuous interactions with infectious microorganisms. Antimicrobial peptides (AMPs) are peptides which protect their hosts against a vast array of microorganisms. These peptides are produced by several species including bacteria, insects, plants, vertebrates and they have been recognized as ancient evolved molecules that have been effectively preserved in mammals. AMPs are expressed on the primary barriers of the organism such as skin and mucosal epithelia, preventing the colonization of host tissues by pathogens. We have previously reported the induction after infection and the cytotoxic activity of potato aspartic proteases (StAPs) towards plant pathogens. Here we show results on the antimicrobial/antitumor activities of these enzymes and of a domain of these enzymes named as StAsp-PSI. StAsp-PSI has structural homology with a family of proteins with antimicrobial/antitumor activity named as SAPLIPs family. Ours results show that StAsp-PSI is able to kill spores of two potato pathogens but not plant cells, in a dose dependent manner. As reported for StAPs (Solanum tuberosum aspartic proteases), StAsp-PSI ability to kill microbial pathogens is dependent on the direct interaction of the protein with the microbial cell wall/or membrane, leading to increased permeability and lysis. Additionally, we demonstrated that, like proteins of SAPLIP family, StAsp-PSI and StAPs are cytotoxic for Gram negative and Gram positive bacteria in a dose dependent manner. The amino acid residues conserved in SP_B (pulmonary surfactant protein B) and StAsp-PSI could explain the cytotoxic activity exerted by StAsp-PSI and StAPs against Gram positive bacteria. On the other hand, results obtained show that StAPs induce apoptosis on Jurkat T cells at short time of incubation in a dose dependent manner. However, not significative effect on the T lymphocytes viability was observed at any time and StAPs concentration assay. StAsp-PSI was able to induce DNA fragmentation, ROS induction and cell cytotoxicity on human breast cancer cells in a dose dependent manner. These results open a new perspective to test these proteins as possible candidates to develop new drugs that would be active against microbes but not against mammalian cells and considerer these proteins as conceptually promising agent in cancer therapy.
Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression.

RNA abundance and DNA copy number are routinely measured in high-throughput using microarray and next-generation sequencing (NGS) technologies, and the attributes of different platforms have been extensively analyzed. Recently, the application of both microarrays and NGS has expanded to include microRNAs (miRNAs), but the relative performance of these methods has not been rigorously characterized. We analyzed three biological samples across six miRNA microarray platforms and compared their hybridization performance. We examined the utility of these platforms, as well as NGS, for the detection of differentially expressed miRNAs. We then validated the results for 89 miRNAs by real-time RT-PCR and challenged the use of this assay as a "gold standard. " Finally, we implemented a novel method to evaluate false-positive and false-negative rates for all methods in the absence of a reference method.
TERATOID HEPATOBLASTOMA

We present the case of a Middle Eastern child, diagnosed and treated at 8 months of age for a hepatic neuroblastoma. After surgical removal of a 7 cm mass of the left liver lobe at our institution when the child was 15 months of age, the tumor was reclassified as a teratoid hepatoblastoma. The tumor was composed of fetal and embryonal hepatic tissue, undifferentiated tissue, and a teratoid background of loose mesenchymal tissue containing osteoid, squamous, and mucinous epithelium. We speculate on the histogenesis of teratoid hepatoblastoma and discuss its association with chemotherapy.
Modeling and Predicting Hearing Aid Outcome

Following a brief tutorial on the application of factor analysis to hearing aid outcome measures, three studies of hearing aid outcome measures in elderly adults are presented and analyzed. Two of the studies were completed at Indiana University (IU-1 and IU-2), and one was a collaborative multisite study by the Veterans Administration and the National Institute of Deafness and other Communication Disorders (NIDCD/VA). IU-1 measured hearing aid outcome in 173 elderly wearers of single-channel, linear, in-the-ear hearing aids with output-limiting compression, whereas IU-2 obtained the same extensive set of outcome measures from 53 elderly wearers of two-channel, wide-dynamic-range compression, in-the-canal hearing aids. In the NIDCD/VA study, 333 to 338 participants wore three single-channel circuits in succession, with each circuit housed within an in-the-ear shell. The three circuits included in that study and in this analysis were: (1) linear with peak clipping, (2) linear with output-limiting compression, and (3) single-channel, wide-dynamic-range compression. Evaluation of the many outcome measures completed in each study using principal components factor analysis revealed that from three (both IU studies) to five (NIDCD/VA study) principal components captured the individual differences in hearing aid outcome. This was independent of hearing aid type (in-the-ear or in-the-canal) and circuitry. Subsequent multiple regression analyses of individual differences in performance along each dimension of hearing aid outcome revealed that these individual differences could be accounted for reasonably well by various prefit variables for some dimensions of outcome, but not others. In general, measures of speech recognition performance were well accounted for by prefit measures, with the best predictors being hearing loss, cognitive performance, and age. Measures of hearing aid usage were less well accounted for by prefit measures, with the most accurate predictor of current hearing aid use being prior hearing aid use. The outcome dimension accounted for most poorly was that associated with hearing aid satisfaction, with subjective measures of aided sound quality being the best predictor of performance along this dimension of hearing aid outcome. Additional multicenter, large-scale studies are needed to develop more complete models of hearing aid outcome and to identify the variables that influence various aspects of hearing aid outcome. It is only through this additional research that it will be possible to optimize outcome for hearing aid wearers.