Miracle-dz/UniFAR-FTUs huggingface dataset:
Preparation and Characterization of Monoclonal Antibody Against Truncated Recombinant Nuclear Matrix Protein ( NMP 22 )

Background: Bladder cancer is a major worldwide health problem. Diagnosis of acute and chronic bladder carcinoma is based on the detection of a number of tumor markers such as nmp22 (nuclear matrix protein 22). Nmp22 is one of the tumor markers used for detecting the recurrence of bladder cancer. Objectives: The aim of this study was to develop hybrid cell producing monoclonal antibodies (MAbs), specifically for nmp22 using hybridoma technology. Materials and Methods: Complete and incomplete adjuvant with recombinant truncated nmp22 antigens were emulsified and injected to BALB/c mice. The spleen was removed and the splenocytes were fused with sp2/0 myeloma cells. Characterization of the produced mAb was carried out. Results: As a result of the fusion, hybridoma cells were produced and exhibited hightiter antibodies. By testing of colons based on the Enzyme Linked Immunosorbent Assay (ELISA), 135 hybridoma colons were selected, out of which eight high titer clones including 54D-6F-2E-36-7H-2A-8E-1D-6D were detected and one of the clones named FPR92 was selected, and the produced mAb was further characterized. The produced antibody belongs to the IgG class and its light chain was kappa. With respect to affinity, the mAb was included as high affinity 3 × 10-7 reacting with NMP22 recombinant protein . The western blot of cancerous bladder tissue showed the presence of 40 and 55 kD proteins as major bands that reacted with this mAb. Conclusions: Based on a double limiting dilution protocol, a type of monoclonal antibody, named FPR92 was produced and char-
Market for Corporate Control, Takeover Defense & Corporate Investment Efficiency-Master thesis Shu Yin

This study analyzes the relationship between market for corporate control and investment efficiency. I find firm with more anti-takeover provisions, suggesting a weaker market for corporate control, are more likely to over-invest in non-capital investments. Besides, this research studies the joint effect of product competition and market for corporate control on firm’s investment. I find in a competitive industry, market for corporate control has a stronger power of reducing the likelihood of investment inefficiency. These results provide evidence on managerial wealth theory that anti-takeover provisions exaggerate investment inefficiency. Product competition and market for corporate control and have a synergic effect in reducing free cash flow for inefficient investment.
Estimating the size of the UK grey seal population between 1984 and 2004 : model selection , survey effort and sensitivity to priors

We used a Bayesian state space modelling framework to fit and compare models of British grey seal population dynamics using regional estimates of pup production from 1984 to 2004. The models allow for a number of different forms of density dependence in either pup survival or fecundity, as well as fitnessdependent movement of recruiting females between regions. There was little difference between models in the adjusted posterior loglikelihoods, meaning that none of the models was obviously best at describing the data. However, this could be a result of the fitting methods used. The estimated adult population size in 2004 varied from 105,000 (95% CI 78141,000) for the simplest density-dependent survival model to 234,000 (95% CI 167344,000) for the simplest density-dependent fecundity model, the other models having intermediate estimates.
Trauma-Focused CBT Informed Music Therapy: Connecting Traumatized Youth with Affective Modulation — Developing a Method

Remediating the impact of trauma experienced by children and adolescents is a focus of psychotherapy treatment. Research in neurology suggests brain development of youths who have experienced trauma is impacted in negative ways. One impact noted is a delay of the development of emotional perception and regulation, where youths may demonstrate an inability to perceive and identify feelings within themselves or others and may experience inappropriate emotional outbursts or emotional withdrawal. Evidence-based practices (EBPs) with specific components and protocols are increasingly preferred by consumers and insurance providers due to research-supported results within expedient timeframes. Music therapy is an evidence-based practice, however literature of its use to treat youths who have experienced trauma is sparse. Using a recognized evidence-based framework, Trauma-Focused Cognitive Behavior Therapy (TF-CBT), this capstone thesis explores the use of music therapy interventions within the Affect Modulation Skills component of the framework. Two individual case studies are examined in which participants engaged in individually relevant music therapy activities designed to enhance identification and expression of feelings and emotions. Results included increased awareness and regulation of feelings and improved interpersonal connections with others as observed by the author and as self-reported by the participants. TF-CBT INFORMED MUSIC THERAPY 3 Trauma-Focused CBT Informed Music Therapy: Connecting Traumatized Youth with Affective Modulation —Developing a Method
Smartphone Habits and Behaviors in Supporting Students Self-Efficacy

The widespread of smartphones usage have increased the convenience of accessing information and knowledge sharing for higher learning students. University’s students are exposed with the multi channels of knowledge from various sources primarily from online learning’s resources. The study examines smartphone habit, internet literacy, and mobile learning in relation to self-efficacy. Self-efficacy refers to the internal forces of a student’s belief in the abilities in utilizing smartphone as educational aid in the context of mobile learning. This study deploys a quantitative approach in assessing the relationship between self-efficacy, internet literacy and smartphone’s habits for of university students. Understanding student self-efficacy is important factor to deliver an effective ways in supporting mobile learning activities. In addition to documenting the findings of self-efficacy and mobile learning, the research also represents a model of internal and external factors that affects student self-efficacy to make mobile learning successful.
R & D Gaps in the Philippines 1

The PIDS Discussion Paper Series constitutes studies that are preliminary and subject to further revisions. They are being circulated in a limited number of copies only for purposes of soliciting comments and suggestions for further refinements. The studies under the Series are unedited and unreviewed. The views and opinions expressed are those of the author(s) and do not necessarily reflect those of the Institute. Not for quotation without permission from the author(s) and the Institute.
POST-SECONDARY AND HIGHER EDUCATION OF INDIGENOUS PEOPLES IN CANADA : HISTORICAL , SOCIAL , ECONOMIC , CULTURAL , FAMILY-RELATED , AND INDIVIDUAL BARRIERS

The article deals with the issues of post-secondary and higher education of indigenous peoples in Canada. The main objectives are defined as the theoretical analysis of scientific and pedagogical literature highlighting different aspects of the problem under research and identifies of the barriers to obtaining postsecondary and higher education by indigenous peoples. The post-secondary and higher education have been studied by foreign and Ukrainian scientists (Т. Andryushchenko, O. Barabash, N. Bidyuk, B. Burtch, M. Busko, J. Friesen, V. Friesen, S. Honcharenko, V. Kirkness, D. Klyne, O. Kotlyakova, T. Kuchai, L. Lukyanova, H. McCue, M. Mendelson, N. Nychkalo, O. Ogiyenko, J. Peters, R. Price, L. Pukhovska, J. Silver, F. Simard, S. Sysoyeva, J. White, I. Zyazyun). The legislative and normative framework of post-secondary and higher education of indigenous peoples in Canada is considered; the statistical data which characterise the indigenous peoples’ participation in post-secondary and higher education programs are presented; the existing barriers to getting post-secondary and higher education by indigenous peoples are analysed and identified as historical, social, economic, cultural, family-related and individual ones. The research methodology comprises theoretical (logical, induction and deduction, comparison, structural and functional, systematic, analysis and synthesis), and applied (discussion, questioning and interviewing) methods. The research results are presented.
Possible Causes of Tooth Wear in Medieval Icelanders

Objectives: The importance of the Icelandic Sagas as a source of information on the way of life and diet habits in Iceland and possibly other Nordic countries 1000 years ago is obvious. Extensive tooth wear in archaeological human skull material worldwide has been blamed on coarse diet. Near volcano Hekla, 66 skeletons dated from before 1104 were excavated from a graveyard. The purpose of this study was to determine the main causes of tooth wear in Icelanders 1000 years ago. Materials and methods: Available were 49 skulls for research. Two methods were used to evaluate tooth wear and seven for age estimation. An attempt was made to determine main causes of tooth wear in the light of likely diet and beverage consumption according to a computer search on food and drink customs described in the Icelandic Sagas. Results: Extensive tooth wear was seen in all groups, increasing with age. The first molars had the highest score with no difference between sexes. It had all the similarities seen in wear from coarse diet. In some instances it had similar characteristics as seen in erosion in modern Icelanders consuming excessive amounts of soft drinks. According to the Sagas, acidic whey was a daily drink and used for preservation of food in Iceland until recently. Conclusions: It is postulated that consumption of acidic drinks and food in addition to a coarse and rough diet, played a significant role in the dental erosion seen in ancient Icelanders.
Influence of ryegrass managements on the physical properties of a Haplohumox

Abstract: The objective of this work was to evaluate the influence of ryegrass (Lolium multiflorum) managements on the physical properties of a Haplohumox, and on the yields of corn and of ryegrass cultivated in succession to corn. The experiment was carried out in a randomized complete block design, with three treatments and three replicates, in which treatments were the different managements of ryegrass under no-tillage for silage, soil cover, and grazing. After nine years of management, samples were collected at 0.00-0.05, 0.05-0.10, 0.10-0.20, and 0.20-0.30-m soil depths, to determine the following soil properties: texture, total organic carbon, soil bulk density, macroporosity, microporosity, total porosity, and resistance to root penetration. The index of structural stability was estimated from texture and total organic carbon data. Maximum soil bulk density and permanent wilting point were also estimated from pedotransfer functions. Corn and ryegrass dry matter yields were determined from plants harvested inside the plot area. Total organic carbon content increased as depth increased. The ryegrass managements in no-tillage system, in succession to corn, does not influence the soil physical properties of a Haplohumox, and maintains high corn and ryegrass yields.
A Survey of Freshwater Macroinvertebrates on Grenada , West Indies

A survey of macroinvertebrates inhabiting the freshwater habitats of Grenada was conducted during May and June of 1996. Qualitative collections were made by sweeping a dip net through the water column and by hand examination of rocks, plants, and other debris submerged in both flowing and standing bodies of fresh water across the island. These collections yielded at least 32 species previously unknown from Grenada. When my collections were combined with those made by previous investigators, the total number of species recorded from the island is expanded to total at least 86. Dominant taxa collected included a few species of gastropods, decapod crustaceans, ephemeropterans, odonates, and hemipterans. Generally this macroinvertebrate fauna is sparse, most likely due to the oceanic origin of Grenada and periodic disturbance of freshwater environments across the island. METHODS Sixteen sampling sites were established in various freshwater habitats across Grenada. Collections were made during May and June of 1996. Water temperature was also recorded from each site at the time of collection. Because data in this investigation was to be compared with that from other small Caribbean islands, it was important that collecting methods were consistent in application from island to island. Several methods of collecting were employed to ensure as many species as possible were captured. Submerged debris, such as stones, leaves, and wood, was carefully examined and inhabitants were picked from the substrate using forceps. A dip net was swept through aquatic vegetation and the water column to capture macroinvertebrates occupying those microhabitats. The microhabitat from which each specimen occurred was noted. A drift net was used at two sites to capture organisms carried in the current during the night. All specimens were preserved in 70% ethanol and Fig. 1. Map indicating location of collecting sites in Grenada. Specific locations, dates, and approximate elevations of collections are listed below Table 1.
The Levels of Using Reading Strategies of the Prospective Teachers

DOI: 10.14686/buefad.381007 Reading comprehension success is increased by monitoring and controlling cognitive process with metacognitive strategies. Metacognitive strategies take their place as reading strategies in reading education. As a result of the development of metacognitive awareness of the students, the use of strategy is consciously settled. It is important that teacher candidates have the ability to use reading strategies in order to improve their students in this respect in the future. In this study, it is aimed to describe the level of use of reading strategies by prospective teachers. The research was carried out in the survey model. A total of 297 teacher candidates participated in the research at Bartin University Faculty of Education, in the departments of primary school, social studies, Turkish, mathematics and science at the 2nd and 3rd grade classes. The data of the study were collected by the Metacognitive Reading Strategies Scale. As a result of the analyses, it was determined that the teacher candidates used reading strategies frequently, there was no meaningful difference in the level of using reading strategies according to the gender, and there were significant differences according to the departments and reading habits. The inclusion of reading strategies in curricula and in the educational process so that prospective teachers can maximize their use of reading strategies; making motivational, cognitive and behavioral contributions by instructors to direct students to read; longitudinal and qualitative research on the subject is recommended based on the findings of the research. Article History: Received Revised Accepted 19.01. 2018 06.02. 2018 20.02. 2018
Potential Molecular and Cellular Mechanisms Underlying the Anti-Inflammatory and Anti-Tumor Properties of Probiotics : Our Experience

A probiotic is defined as a “live microorganism which when administered in adequate amounts confers a health benefit on the host”. There is a growing interest in probiotics within the scientific community, with consumers, and in the food industry. Thus, a large body of literature exploring the mechanisms of action of probiotic strains and their effects on human health is actually available. In the last twenty years, our research group has been pledged to study the cellular and molecular mechanisms underlying the beneficial effects of selected strains of probiotics. Here, we retrace the most significant stages of our research on probiotics, reporting, in particular, in vitro and in vivo findings regarding their antiinflammation and anti-cancer properties.
Assessment of Genetic Variability for Some Hyoscymus Species Using Biochemical and Molecular Markers

Hyocyamus sp. is an important medicinal plant belonging to the Solanaceae family. It contains tropane alkaloids, mainly hyoscyamine and scopolamine, which have medical usage. The primary objective of this study was to compare between proteins, isozymes as biochemical markers and RAPD-PCR molecular markers to assess the genetic diversity and relationships among five Hyoscyasmus species. SDS-PAGE for total soluble protein and PPO-isozyme analysis revealed low degree of polymorphism. In addition, some species from Hyocyamus sp. did not show any markers. A high degree of polymorphism was detected among the five Hyoscyasmus species through five random primers used for RAPD-PCR marker analysis. Fifteen monomorphic bands and 37 polymorphic distinct fragments (71.15% polymorphism) were revealed in the five Hyocyamus sp. with five primers. In addition, primer-3 and primer-5 were highly polymorphic (88.89 and 83.33% polymorphism, respectively). Moreover, primers 2, 4 and 1 were moderately polymorphic (69.23, 62.50 and 50% polymorphism, respectively). The largest number of RAPD-PCR specific markers scored in H. muticus (eight markers), followed by, H. niger and H. gyoerffyi which were equal in specific marker number (three markers), while the lowest number from specific bands existed in both H. albus and H. aureus (two markers). Consequently, biochemical markers could not be used alone to differentiate between the tested Hyoscyasmus species. On the contrary, molecular markers are useful tools to assess the genetic diversity of five Hyocyamus
Can AMPK mediated suppression of mTORC 1 explain the concurrent training effect ?

Endurance and resistance exercise are divergent modes of exercise training which each drive mode specific adaptive responses. Some of these adaptations are mutually exclusive, whilst others drive divergent effects on muscle contractile properties, mass and metabolic function. When both resistance and endurance exercise training are employed together, a process termed ‘concurrent training’, there appears to be a reduction in skeletal muscle adaptive potential. In real world terms this is evident in decathletes, in which personal bests represent approximately a 25% reduction compared to competitors from individual events. This review will detail the molecular pathways thought to drive the resistance and endurance training response and discuss recent evidence addressing the cross talk between these molecular pathways. Ultimately we will discuss why, in our opinion, the molecular events currently proposed to cause interference in skeletal muscle adaptation to concurrent training are an inadequate explanation for the repression on strength gains observed. Citation: Hamilton DL, Philp A (2013) Can AMPK mediated suppression of mTORC1 explain the concurrent training effect. Cellular and Molecular Exercise Physiology 2(1): e4 doi: 10.7457/cmep.v2i1. e4 Editor: Adam Sharples PhD. Received: June 10, 2013; Accepted August 9, 2013; Published August 28, 2013 Copyright: © 2013 Hamilton DL, Philp A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and work is properly cited. Competing Interests: The authors have declared that no competing interests exist. * E-mail: d.l. hamilton@stir.ac. uk Current address: Health and Exercise Sciences Research Group, University of Stirling, Stirling, UK, FK9 4LA.
Impact of the MJO on the Gulf of Carpentaria during the monsoon

The Madden-Julian Oscillation (MJO) is an atmospheric phenomenon but the associated precipitation, convection, and wind anomalies also interact with the ocean surface. Broad scale patterns of intraseasonal sea level anomalies have been detected worldwide (Fu 2003) and are largely driven by surface wind stress. Oliver and Thompson (2010) found that sea level from many ocean regions, including the equatorial Pacific, the west coast of the American continent, the Gulf of Carpentaria and the northeastern Indian Ocean, have significant correlations with the MJO. Sea level and circulation in the Gulf of Carpentaria have been shown to be related to the MJO during the Australian-Indonesian monsoon season (Oliver and Thompson 2011) and these results are reviewed here as well as a discussion on the predictability of Gulf of Carpentaria sea level.
Multidimensional Independent Component Analysis with Higher-order cumulant matrices for vector sources with possibly differing dimensions

The paper addresses the separation of multidimensional sources, with possibly diﬀerent dimensions, by means of higher-order cumulant matrices. First, it is rigorously proved, in a general setting, that contracted cumulant matrices of any order are all block-diagonalizable in the same basis. Second, a family of joint block-diagonalization algorithms is proposed that separate multidimensional sources by combining contracted cumulant matrices of arbitrary orders. Third, a speciﬁc solution is given to determine the source dimensions when they are unknown but all diﬀerent. The performances of the proposed algorithms are compared between them and with algorithms of the literature based on orders 3 and 6.
Characterization of the CDPD-mannitol biosynthetic pathway in Streptococcus pneumoniae 35 A

Quan Wang2,3,4, Yanli Xu2,3,4, Andrei V Perepelov5, Yuriy A Knirel5, Peter R Reeves6, Alexander S Shashkov5, Peng Ding2,3,4, Xi Guo2,3,4, and Lu Feng1,2,7,8 TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, People’s Republic of China; Key Laboratory of Molecular Microbiology and Technology; and The Engineering and Research Center for Microbial Functional Genomics and Detection Technology, Ministry of Education, Tianjin, People’s Republic of China; N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russian Federation; School of Molecular and Microbial Biosciences (G08), University of Sydney, Sydney, NSW 2006, Australia; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, People’s Republic of China; and Tianjin Research Center for Functional Genomics and Biochip, Tianjin, People’s Republic of China
Mobile Banking Service Quality and Customer Satisfaction in Bangladesh : An Analysis

The study has been conducted to investigate how the SERVQUAL dimensions influence customer satisfaction in the mobile banking industry of Bangladesh. The researchers have used customer satisfaction as the dependent variable, while five dimensions of service quality model (namelytangibles, reliability, responsiveness, assurance and empathy) are taken as independent variables. A questionnaire had been developed and distributed among 166 customers of five mobile banking service providers of Bangladesh. The respondents were selected from Dhaka city by using Simple Random Sampling. Results were subsequently analyzed by using descriptive statistics, Pearson correlation and multiple regressions. Results showed that the four variables tangible, reliability, responsiveness, and empathy have significant positive relationship with customer satisfaction. However, the assurance factor has no significant relationship with customer satisfaction. The results imply that firms should focus upon tangible, reliability, responsiveness, and empathy variable as these are the key indicators of customer satisfaction of mobile banking services.