Miracle-dz/UniFAR-FTUs huggingface dataset:
SELECTION FOR ALTERNATIVE MALE REPRODUCTIVE TACTICS ALTERS INTRALOCUS SEXUAL CONFLICT

Intralocus sexual conflict (IASC) arises when fitness optima for a shared trait differ between the sexes; such conflict may help maintain genetic variation within populations. Sex‐limited expression of sexually antagonistic traits may help resolve the conflict, but the extent of this resolution remains a subject of debate. In species with alternative male reproductive tactics, unresolved conflict should manifest more in a more sexually dimorphic male phenotype. We tested this prediction in the bulb mite (Rhizoglyphus robini), a species in which aggressive fighters coexist with benign scramblers. To do this, we established replicated lines in which we increased the proportion of each of the alternative male morphs using artificial selection. After approximately 40 generations, the proportion of fighters and scramblers stabilized at >0.9 in fighter‐ and scrambler‐selected lines, respectively. We then measured several female fitness components. As predicted by IASC theory, female fecundity and longevity were lower in lines selected for fighters and higher in lines selected for scramblers. This finding indicates that sexually selected phenotypes are associated with an ontogenetic conflict that is not easily resolved. Furthermore, we suggest that IASC may be an important mechanism contributing to the maintenance of genetic variation in the expression of alternative reproductive tactics.
The hNT Human Neuronal Cell Line Survives and Migrates into Rat Retina

The present studies were undertaken to determine if hNT cells can survive in the vitreous of the eye and migrate into the retina. The hNT neuronal cell line represents a uniform source of human tissue that may be of use in retinal grafts. hNT cells stored in liquid nitrogen were thawed and labeled with the fluorescent dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine Perchlorate (DiI). Thirty thousand cells in 1 μL were injected epiretinally in rat. At survival times of 3, 14, 28, or 56 days, retinal sections were examined quantitatively by epifluorescence to reveal DiI-labeled cells. hNT cells survived in the vitreous at all time points without evidence of vascularization. At 3 days, essentially no hNT cells were found in deep retina, and only very few were attached to retina. At days 14, 28, and 56, hNT cells were found to cluster on the vitreal/retinal interface, and in deeper layers. The clusters of hNT cells took on the shape of a funnel at 14 days, and inverted funnel at 28 days, and by 56 days, populated the photoreceptor layer as a stratum. It is possible that hNT cells took on the morphology and function of photoreceptors. These results suggest that hNT cells injected epiretinally survive in the vitreous at least 56 days, migrate to the retinal/vitreous interface, and may migrate through the retina. This system permits the independent and quantitative evaluation of survival and migratory trophic responses. © 1998 Elsevier Science Inc.
Habitat use and activity with new records of the agile snouted tree frog ([I]Scinax agilis[/I]) on the north coast of Bahia, Brazil.

The agile snouted tree frog Scinax agilis (Anura, Hylidae) is one of the restinga frog species, with reported distribution in the Espírito Santo, Alagoas, Sergipe and Bahia states, especially in sand dune habitats, known locally as restinga. On the north coast of Bahia, north-eastern Brazil, it is known, according to literature, only from the cities Camaçari and Mata de Sao Joao. In this study we fill the geographic distribution gap in the region including presence in six new localities and cities. We also report findings on diurnal and nocturnal activity patterns and the use of microhabitats at the different vegetation type habitats. We found S. agilis in dry forest, scrub and beach vegetation and also at the temporary or permanently flooded river plains. Most records (52.2 %) were on river plains during daytime surveys mainly from 1200 to 1800 hours. Bromeliads were the most frequently used microhabitat (45.4%) followed by aquatic plants. The agile snouted tree frog association with flooded river plains and associated plant communities reinforces the urgent need for conservation measures to preserve the restinga remnants in the region. Figure 1: Adult agile snouted tree frog (Scinax agilis) from Busca Vida, city of Camaçari. Adults averaged SVL = 130 mm RESEARCH ARTICLE The Herpetological Bulletin 129, 2014: 19-23
Morphometric responses and tolerance of pomegranate seedlings irrigated with saline water

ABSTRACT Information on the production of seedlings and initial growth of pomegranate irrigated with saline water is scarce in the Brazilian semi-arid region. The objective of this study was to assess the effect of irrigation water salinity levels on the growth and tolerance of pomegranate seedlings. The experiment was conducted in a greenhouse, located at the Federal Rural University of the Semi-Arid Region, Mossoró, RN, Brazil. A randomized block design with five treatments [electrical conductivities of irrigation water (ECw) of 0.6, 3.0, 6.0, 9.0, 12.0 dS m-1] and five replicates, with three plants per plot, was used. At 120 days after sowing, the following characteristics were evaluated: shoot, root and total lengths (cm); stem diameter (mm), dry mass of stem, leaves, root system and total (g); ratio between root and shoot dry mass; Dickson quality index and salinity tolerance. Growth and dry biomass accumulation in pomegranate seedlings were compromised with increased salinity in irrigation water, but with a lower intensity when subjected up to EC of 6.0 dS m-1. Regarding the criterion of evaluation of tolerance to salinity, pomegranate seedlings were moderately tolerant to salinity.
Use of a simulation-based advanced resuscitation training curriculum: Impact on cardiopulmonary resuscitation quality and patient outcomes

Background Despite a continued focus on improved cardiopulmonary resuscitation quality, survival remains low from in-hospital cardiac arrest. Advanced Resuscitation Training has been shown to improve survival to hospital discharge and survival with good neurological outcome following in-hospital cardiac arrest at its home institution. We sought to determine if Advanced Resuscitation Training implementation would improve patient outcomes and cardiopulmonary resuscitation quality at our institution. Methods This was a prospective, before–after study of adult in-hospital cardiac arrest victims who had cardiopulmonary resuscitation performed. During phase 1, standard institution cardiopulmonary resuscitation training was provided. During phase 2, providers received the same quantity of training, but with emphasis on Advanced Resuscitation Training principles. Primary outcomes were return of spontaneous circulation, survival to hospital discharge, and neurologically favorable survival. Secondary outcomes were cardiopulmonary resuscitation quality parameters. Results A total of 156 adult in-hospital cardiac arrests occurred during the study period. Rates of return of spontaneous circulation improved from 58.1 to 86.3% with an adjusted odds ratios of 5.31 (95% CI: 2.23–14.35, P < 0.001). Survival to discharge increased from 26.7 to 41.2%, adjusted odds ratios 2.17 (95% CI: 1.02–4.67, P < 0.05). Survival with a good neurological outcome increased from 24.8 to 35.3%, but was not statistically significant. Target chest compression rate increased from 30.4% of patients in P1 to 65.6% in P2, adjusted odds ratios 4.27 (95% CI: 1.72–11.12, P = 0.002), and target depth increased from 23.2% in P1 to 46.9% in P2, adjusted odds ratios 2.92 (95% CI: 1.16–7.54, P = 0.024). Conclusions After Advanced Resuscitation Training implementation, there were significant improvements in cardiopulmonary resuscitation quality and rates of return of spontaneous circulation and survival to discharge.
Growth performance and nutrient digestibility of growing pigs fed a mixture of palm kernel meal and cassava peel meal

In a 42 days feeding trials, twenty growing crossbred pigs with mean initial body weights of 16 ± 0.8kg were assigned randomly to 5 dietary treatment groups of four pigs each. A basal diet was formulated. The basal diet was based on cassava flour, maize offals, soyabean meal and brewer’s dried grain (BDG). A mixture of cassava peel meal and (CPM) and palm kernel meal (PKM) replaced the BDG fraction in the basal diet in the ratio 2:0, 0:2, 1:1 and 1.75:1.75 in diets II, III, IV and V respectively. Each diet was offered on ad libitum basis. Pigs were individually weighed on weekly basis until the end of the experiment. Daily voluntary feed intake was monitored. Growing pigs responded non-significantly (p> 0.05) to the mixture of different fibre sources in body weight gain, feed conversion ratio and protein efficiency ratio except for feed intake, which varied significantly (p< 0.05). Data on final body weight was described using the linear regression method. The slope of regression of final body weight (y) (kg/pig/week) depending on weeks (kg/week) was highest on diet V (3.525 ± 0.1842) and lowest on diet II (2.074 ± 0.1742). Digestibility values also varied significantly (p< 0.05) with ether extract having the average digestibility value (%) of 90.32 and ash the lowest value of 53.60.
HYBRIDIZATION OF BOMBINA BOMBINA AND B. VARIEGATA (ANURA, DISCOGLOSSIDAE) AT A SHARP ECOTONE IN WESTERN UKRAINE: COMPARISONS ACROSS TRANSECTS AND OVER TIME

Abstract Bombina bombina and B. variegata are two anciently diverged toad taxa that have adapted to different breeding habitats yet hybridize freely in zones of overlap where their parapatric distributions meet. Here, we report on a joint genetic and ecological analysis of a hybrid zone in the vicinity of Stryi in western Ukraine. We used five unlinked allozyme loci, two nuclear single nucleotide polymorphisms and a mitochondrial DNA haplotype as genetic markers. Parallel allele frequency clines with a sharp central step occur across a sharp ecotone, where transitions in aquatic habitat, elevation, and terrestrial vegetation coincide. The width of the hybrid zone, estimated as the inverse of the maximum gradient in allele frequency, is 2.3 km. This is the smallest of four estimates derived from different clinal transects across Europe. We argue that the narrow cline near Stryi is mainly due to a combination of habitat distribution and habitat preference. Adult toads show a preference for either ponds (B. bombina) or puddles (B. variegata), which is known to affect the distribution of genotypes within the hybrid zones. At Stryi, it should cause a reduction of the dispersal rate across the ecotone and thus narrow the cline. A detailed comparison of all five intensively studied Bombina transects lends support to the hypothesis that habitat distribution plus habitat preference can jointly affect the structure of hybrid zones and, ultimately, the resulting barriers to gene flow between differentiated gene pools. This study also represents a resampling of an area that was last studied more than 70 years ago. Our allele-frequency clines largely coincide with those that were described then on the basis of morphological variation. However, we found asymmetrical introgression of B. variegata genes into B. bombina territory along the bank of a river.
Epidemiology and course of acute upper gastro‐intestinal haemorrhage in four French geographical areas

Objective To compare incidence rates and epidemiological characteristics of acute upper gastrointestinal haemorrhage (AUGIH) in France with those of other European studies. Design Population‐based multi‐centre prospective survey. Setting 29 public hospitals and 96 private specialists in gastroenterology in four administrative areas in France during 1996. Subjects A total of 2133 AUGIH patients 18 years and over were included in the six‐month study. Outcome measures Incidence and mortality. Results The overall incidence in France was 143 cases per 100 000 persons per year, classified as out‐patients (16%), emergency admissions (59%) and in‐patients (25%). The incidence rates increased with age except for in‐patients, and were higher in males. Peptic ulcer (36.6%), varices (13.7%) and erosive disease (12.3%) were the most frequent diagnoses. In 677 patients (31.7%), aspirin, antiinflammatory drugs or corticosteroids were taken on the 7 days before bleeding. The overall mortality (out‐patients excluded) was 14.3% (10.7% for emergency patients and 23% for in‐patients). Mortality was associated with comorbidities (especially malignancies, cirrhosis, asthma or respiratory deficiency), was lower in emergency patients using non‐steroid anti‐inflammatory drugs, and higher in in‐patients using corticosteroids. Conclusions In France, patients with AUGIH are frequently managed as out‐patients. Gastrotoxic drug use is frequently associated with AUGIH and constitutes a strategic opportunity for preventive treatment. Discrepancies between countries are not clearly explained either by demographic factors or by drug use, but this may be related to the emphasis on AUGIH in in‐patients. Eur J Gastroenterol Hepatol 12:175‐181 © 2000 Lippincott Williams & Wilkins European Journal of Gastroenterology & Hepatology 2000, 12:175‐181
Identification and characterization of an essential gene in Listeria monocytogenes using an inducible gene expression system

The Listeria monocytogenes gene lmo1594 is a homolog of the Bacillus subtilis cell division gene ezrA. EzrA is a negative regulator of FtsZ ring formation, which is required for efficient cell division as it regulates the frequency and position of Z-rings in the cell and prevents aberrant polar cell division. Previously identified as a putative high pressure (HP) resistance mechanism; conferring enhanced barotolerance when heterologously expressed against an Escherichia coli background; the aim of the current study was to investigate whether lmo1594 plays a role in listerial barotolerance. When the creation of a deletion mutant proved unsuccessful, the role of lmo1594 was addressed by creating a conditional knockout mutant which demonstrated that the gene is in fact essential for cell survival and growth in L. monocytogenes. In order to investigate the effect of lmo1594 on barotolerance, the gene was over-expressed. The over-expression of lmo1594 increased survival levels in L. monocytogenes treated at 300 MPa, but survival levels similar to those of the wild-type strain were observed when treated at a higher pressure (≥400 MPa). In conclusion, this study reveals for the first time that lmo1594 is absolutely essential for listerial cell growth and survival, and also plays an important role in listerial barotolerance.
Hemodynamic Regulation of CD34+ Cell Localization and Differentiation in Experimental Aneurysms

Objectives—Bone marrow-derived vascular progenitor cells (CD34+) are present in human and animal models of abdominal aortic aneurysm (AAA) disease. These preterminally differentiated cells may modulate disease resistance. We examined the influence of variable hemodynamic conditions on progenitor cell localization and differentiation in experimental AAAs. Methods and Results—Murine AAAs were created via porcine pancreatic elastase (PPE) infusion. AAA blood flow was increased by aortocaval fistula (ACF) formation (HF-AAA), decreased via left iliac ligation (LF-AAA), or left unchanged (NF-AAA). ACF creation increased flow by 1700%, whereas iliac ligation decreased flow 79% compared with baseline (0.6±0.1 mL/min). Wall shear stress (WSS) increased or decreased accordingly, and remained elevated (9.2±2.0 dynes/cm2) in HF-AAA 14 days after PPE infusion. CD34+ cells were identified throughout the aortic wall in all flow conditions. Seven days after PPE infusion, HF-AAAs had more CD34+ cells than LF-AAA (187±10 versus 155±7 CD34+ cells/cross sectional, P<0.05), more medial smooth muscle cells, fewer infiltrative macrophages, and a smaller diameter than LF-AAA. LF-AAAs also contained more adventitial capillaries (CD34+ capillaries 181±12 versus 89±32/cross-sectional area in HF-AAA, P<0.05). The total progenitor cell/capillary index (CD34+ capillary plus CD31+ capillary/cross sectional area) was higher in LF-AAA (282±31 versus 129±47, P<0.05). Vascular endothelial (VEGF) and platelet-derived growth factor (PDGF) expression varied directly with capillary density between groups. Increased granulocyte-macrophage colony-stimulating factor (GM-CSF) expression was also present in LF-AAAs. Conclusions—Hemodynamic conditions influence CD34+ cell localization and differentiation in experimental AAA. Adventitial capillary angiogenesis may augment inflammation and disease progression. Modulating cell lineage differentiation of mature progenitor cells may represent a novel therapeutic strategy to maintain medial cellularity and extracellular matrix integrity in AAA disease.
The Role of Semantic Knowledge in Short-term Memory

In this paper we examine the role of stored semantic knowledge in recall from short-term memory. We assessed the performance of a patient (FK), who showed a consistent lack of semantic knowledge for some words (‘unknown’) but not others (‘known’) on a range of serial recall tasks using both spoken and written words. Overall, FK was significantly better at recalling lists of known compared with unknown words. His recall of unknown words was characterized by numerous phonological errors, such as repeating ‘bear skunk’ as ‘bunk scare’. FK showed a relatively normal primacy effect in immediate recall, but a striking lack of a recency effect. This pattern of performance is useful for constraining theoretical accounts of language production and verbal short-term memory and for understanding the role that long-term semantic knowledge may play in maintaining information in short-term memory.
Assessing the Relationship between Personal Particulate and Gaseous Exposures of Senior Citizens Living in Baltimore, MD

ABSTRACT We conducted a multi-pollutant exposure study in Baltimore, MD, in which 15 non-smoking older adult subjects (>64 years old) wore a multi-pollutant sampler for 12 days during the summer of 1998 and the winter of 1999. The sampler measured simultaneous 24-hr integrated personal exposures to PM25, PM10, SO4 2-, O3, NO2, SO2, and exhaust-related VOCs. Results of this study showed that longitudinal associations between ambient PM2.5 concentrations and corresponding personal exposures tended to be high in the summer (median Spearman's r = 0.74) and low in the winter (median Spearman's r = 0.25). Indoor ventilation was an important determinant of personal PM2.5 exposures and resulting personal-ambient associations. Associations between personal PM25 exposures and corresponding ambient concentrations were strongest for well-ventilated indoor environments and decreased with ventilation. This decrease was attributed to the increasing influence of indoor PM2 5 sources. Evidence for this was provided by SO4 2-measurements, which can be thought of as a tracer for ambient PM25. For SO4 2-, personal-ambient associations were strong even in poorly ventilated indoor environments, suggesting that personal exposures to PM2.5 of ambient origin are strongly associated with corresponding ambient concentrations. The results also indicated that the contribution of indoor PM2.5 sources to personal PM2.5 exposures was lowest when individuals spent the majority of their time in well-ventilated indoor environments. Results also indicate that the potential for confounding by PM2.5 co-pollutants is limited, despite significant correlations among ambient pollutant concentrations. In contrast to ambient concentrations, PM2.5 exposures were not significantly correlated with personal exposures to PM2.5-10, PM2.5 of non-ambient origin, O3, NO2, and SO2. Since a confounder must be associated with the exposure of interest, these results provide evidence that the effects observed in the PM2.5 epidemiologic studies are unlikely to be due to confounding by the PM2.5 co-pollutants measured in this study.
Heterogeneous histopathology of cortical microbleeds in cerebral amyloid angiopathy

Objective: To investigate the histopathologic substrate of microbleeds detected on 7T postmortem MRI in autopsy cases with severe cerebral amyloid angiopathy (CAA) and Alzheimer pathology. Methods: Five decedents (mean age at death 79.6 ± 5.7 years) with documented severe CAA and Alzheimer pathology on standard neuropathologic examination were selected from a local database. Formalin-fixed coronal brain slices were scanned at 7T MRI, including high-resolution T2- and T2*-weighted sequences. Representative microbleeds from each case were sampled for histopathologic analysis, including the presence of blood, blood breakdown products, and markers of ischemic tissue injury. Results: On MRI, we identified >300 cortical and 4 subcortical microbleeds. Two out of 15 sampled cortical microbleeds corresponded histologically to erythrocytes (suggestive of recent hemorrhages), 4 to vasculopathies (fibrinoid necrosis in 3 and a cavernoma) without substantial parenchymal tissue injury, and 9 to accumulations of iron-positive siderophages without erythrocytes (suggestive of old hemorrhages) combined with mild to moderate degrees of chronic ischemic tissue injury. Conclusions: This study provides evidence for heterogeneous pathologic substrates and possibly different pathophysiologic mechanisms underlying MRI-observed cortical microbleeds in the context of advanced CAA and Alzheimer disease.
Persistence as a function of locus of control and Expectancy of goal attainment

180 male students of 10+1&10+2 were tested on Rotter’s scale of locus of control and experimentally induced situations of probability of success by giving specific instructions. An Insoluble figure--‘perceptual reasoning task’ was used to test persistence of externally controlled Ss & internally controlled Ss in three experimental conditions i.e.P.25 (difficult task), P.50(intermediate task) &P.75(easy task). Persistence scores were taken as time spent by Ss on perceptual reasoning task. Analysis of variance & t-test were used for analysis of data. Findings concluded that-----(1) internally controlled Ss persisted longer at P.50&P.75 in comparison to P.25. (2) externally controlled Ss persisted longer at P.50 in comparison to P.25&P.75(3)there was no significant difference between internally controlled Ss and externally controlled Ss at P.50.