CONCEPTION IN WEST AFRICAN DWARF DOES INSEMINATED WITH NORMAL SALINE DILUTED BUCK SEMEN

ABSTRACT This study investigated fertility response of West African Dwarf (WAD) does artificially inseminated with semen diluted with normal saline and the dilution ratio for achieving an optimum conception. Semen was collected from seven proven adult WAD bucks via electro ejaculation method and pooled. The semen collected was divided into 5 portions and each diluted with normal saline at 1:0 (T1, control), 1:1(T2), 1:2 (T3), 1:3 (T4) and 1:4 (T5) corresponding to five treatments. The treatments were evaluated for motility, livability and concentration. Twenty eight does aged 12-24 months with an average weight of 12.15±1.51kg were allotted to the five treatment groups with unequal replicates in a completely randomised design. Oestrus in the does was synchronised using prostaglandin (PGF2α) (Lutalyse (R) Pharmacia and Upjohn CO. NY). Oestrus was detected at 72 to 96 hours and insemination was carried out at 78 th and 90 th hours with respective treatments at 0.4 mL of diluted semen through the cervix using insemination gun. Conception was monitored by inspection of those that returned to oestrus and with the use of ultrasound scanner at day 65. Collected data were subjected to descriptive statistics and ANOVA. Results indicated that spermatozoa motility ranged from 81.67±2.87% (T1) to 90.00±5.00% (T4). T1 and T5 had lower motility than other treatments. Spermatozoa concentration ranged from 6.38±0.51x10 9 /mL (T5) to 31.87±2.58x10 9 /mL (T1). The number of motile sperm cells inseminated ranged from 5.33±0.59x10 9 /mL (T5) to 26.05±2.75x10 9 /mL (T1). Total live spermatozoa ranged from 5.94±0.58x10 9 /mL(T5) to 29.48±1.51x10 9 /mL (T1). Does inseminated with semen diluted at ratio 1:1 (T2) had the highest percentage conception, while T1, T4 and T5 had the least percentage conception. The present study showed that conception was enhanced when normal saline was used to dilute goat semen at ratio of 1:1, while dilution up to 1:4 also had a comparable percentage conception with the undiluted semen. RESUMEN En este estudio se investigo la respuesta a la fertilidad en cabras West African Dwarf (WAD) que se inseminaron artificialmente con semen diluido en solucion salina normal y la relacion de dilucion para lograr un nivel de concepcion optimo. El semen se recolecto de siete machos WAD adultos probados, mediante electroeyaculacion y fue mezclado. El semen recolectado se dividio en 5 alicuotas y cada una se diluyo con solucion salina normal a 1:0 (T1, control), 1:1 (T2), 1:2 (T3), 1:3 (T4) y 1:4 (T5). ) correspondiente a cinco tratamientos. Los tratamientos fueron evaluados por motilidad, vitalidad y concentracion. Se usaron veintiocho cabras, con una edad entre 12 a 24 meses y un peso promedio de 12.15±1.51 kg, para los cinco grupos de tratamientos con replicas desiguales en un diseno completamente al azar. El celo se sincronizo usando prostaglandina (PGF2α) (Lutalyse (R) Pharmacia y Upjohn CO. NY) y se detecto a las 72 a 96 horas y la inseminacion se realizo entre 78 y 90 horas con 0,4 ml de semen diluido y a traves del cuello uterino utilizando una pistola de inseminacion. La concepcion se monitorizo mediante la deteccion del celo y con el uso de ultrasonografia el dia 65. Los datos recopilados se sometieron a estadisticas descriptivas y ANOVA. Los resultados indicaron que la motilidad de los espermatozoides oscilo entre 81.67±2.87% (T1) y 90.00±5.00% (T4). T1 y T5 tuvieron menor motilidad que otros tratamientos. La concentracion de espermatozoides oscilo entre 6.38±0.51x10 9 /mL (T5) y 31.87±2.58x10 9 /mL (T1). El numero de espermatozoides motiles inseminados vario desde 5,33±0,59x10 9 /ml (T5) hasta 26,05 2,75x10 9 /mL (T1). El total de espermatozoides vivos oscilo entre 5.94±0.58x10 9 /mL (T5) y 29.48±1.51x10 9 /mL (T1). Las hembras inseminados con semen diluido en una proporcion de 1:1 (T2) tuvieron el mayor porcentaje de concepcion, mientras que T1, T4 y T5 tuvieron el menor porcentaje de concepcion. El presente estudio muestra que la concepcion se mejora cuando se utiliza solucion salina normal para diluir el semen en una proporcion de 1:1, mientras que la dilucion 1:4 tuvo una concepcion porcentual comparable con el semen sin diluir.