Miracle-dz/UniFAR-FTUs huggingface dataset:
Understanding AWE: Can a Virtual Journey, Inspired by the Overview Effect, Lead to an Increased Sense of Interconnectedness?

Immersive technology, such as virtual reality, provides us with novel opportunities to create and explore affective experiences with a transformative potential mediated through awe. The profound emotion of awe, that is experienced in response to witnessing vastness and creates the need for accommodation that can lead to restructuring of one's worldview and an increased feeling of connectedness. An iconic example of the powers of awe is observed in astronauts who develop instant social consciousness and strong pro-environmental values in response to the overwhelming beauty of Earth observed from space. Here on Earth, awe can also be experienced in response to observing vast natural phenomenon or even sometimes in response to some forms of art, presenting vast beauty to its audience. Can virtual reality provide a new powerful tool for reliably inducing such experiences? What are some unique potentials of this emerging medium? This paper describes the evaluation of an immersive installation "AWE" – Awe-inspiring Wellness Environment. The results indicate that the experience of being in "AWE" can elicit some components of awe emotion and induce minor cognitive shifts in participant's worldview similar to the Overview Effect, while this experience also has its own attributes that might be unique to this specific medium. Comparing the results of this study to other virtual environments designed to elicit Overview Effect provides insights on the relationship between design features and participant's experience. The qualitative results highlight the importance of perceived safety, personal background and familiarity with the environment, and the induction of a small visceral fear reaction as a part of the emotional arc of the virtual journey – as some of the key contributers to the affective experience of the immersive installation. Even though the observed components of awe and a few indications of cognitive shift support the potential of Virtual Reality as a transformative medium, many more iterations of the design and research tools are required before we can achieve and fully explore a profound awe-inspiring transformative experience mediated through immersive technologies.
Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study

Background: Staphylococcus aureus is a major human pathogen. Panton-Valentine leukocidin (PVL) is a virulence factor produced by some strains that causes leukocyte lysis and tissue necrosis. PVL-associated S. aureus (PVL-SA) predominantly causes skin and soft-tissue infections (SSTIs) but can also cause invasive infections such as necrotizing pneumonia. It is carried by both community-associated methicillin susceptible S. aureus (CA-MSSA) and methicillin resistant S. aureus (CA-MRSA). This study aims to determine the prevalence of PVL-SA among patients seen at an urban Gambian hospital and associated antibiotic resistance. Methods: Archived clinical S. aureus (70 invasive bacteraemia and 223 non-invasive SSTIs) from 293 patients were retrieved as well as relevant data from clinical records where available. Antibiotic susceptibility was assessed using disc diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines. Genomic DNA was extracted and the presence of lukF and lukS PVL genes was detected by conventional gel-based PCR. Result: PVL-SA strains accounted for 61.4% (180/293) of S. aureus isolates. PVL prevalence was high in both Gambian bacteraemia and SSTIs S. aureus strains. Antimicrobial resistance was low and included chloramphenicol (4.8%), cefoxitin (2.4%), ciprofloxacin (3.8%), erythromycin (8.9%), gentamicin (5.5%) penicillin (92.5%), tetracycline (41.0%), and sulfamethoxazole-trimethoprim (24.2%). There was no association of PVL with antimicrobial resistance. Conclusion: PVL expression is high among clinical S. aureus strains among Gambian patients. Reporting of PVL-SA clinical infections is necessary to enable the monitoring of the clinical impact of these strains in the population and guide prevention of the spread of virulent PVL-positive CA-MRSA strains. SUMMARY Staphylococcus aureus (S. aureus) is a major human pathogen with several virulence factors. We performed a retrospective analysis to investigate the prevalence of one such virulence factor (PVL) amongst clinical S. aureus samples. We found a high prevalence in our setting but antimicrobial resistance including methicillin resistance was low.
Endothelium-Derived Relaxing Factor: Presence in Pulmonary and Systemic Arteries of the Newborn Guinea Pig

ABSTRACT: Endothelium-derived relaxing factor (EDRF), believed to be nitric oxide or a compound that releases nitric oxide, is a potent vasodilator produced by some arteries in response to acetylcholine (ACh) and bradykinin (BK). ACh and BK are potent dilators of perinatal pulmonary and systemic arteries. The objectives of this study were to determine if EDRF is present in newborn vessels and if EDRF mediates the vasodilator actions of ACh and BK. Arterial rings from newborn guinea pigs, 1 to 3 d old, were obtained from a branch of the main pulmonary artery and the descending aorta for isometric force bioassays. At their optimal resting tension, the rings were preconstricted with phenylephrine 10-5 M in Krebs- Henseleit solution before adding incremental doses of ACh or BK. If the endothelium was intact, ACh (10s M) relaxed pulmonary arteries and aortas (64 ± 7%, 72 ± 9% relaxation, respectively, mean ± SE). ACh-induced relaxation (ACh 10-5 M) in the pulmonary artery and aorta, respectively, was significantly (p < 0.05) attenuated by 1) endothelial removal (11 ± 9%, 28 ± 10%) by rubbing the ring lumen; 2) methylene blue, 10-6 M, (6 ± 8%, 7 ± 3%) that inhibits EDRF-associated cGMP production in smooth muscle; and 3) methemoglobin, 10-5 M, (13 ± 9%, 17 ± 7%) that binds EDRF. The results for BK were similar to ACh for the pulmonary artery but BK did not relax the aorta. Indomethacin diminished relaxation of the pulmonary artery and aorta to the submaximal dose (10-5M) of ACh but indomethacin did not effect the relaxation to ACh 10-4 M or BK. We conclude that EDRF is produced in the guinea pig pulmonary artery and descending aorta at birth and that EDRF is a mediator of the vasodilator actions of ACh and BK. Vasodilation by ACh may also involve activation of the cyclooxygenase pathway.
Competitive Psychological Disposition and Perception of Performance in Young Female Soccer Players

The athletes’ psychological disposition is a factor that is increasingly considered by researchers as a key to sports performance, even as a mediator between the physical, technical and tactical abilities of the athlete and their competitive performance, thus acquiring great relevance in training and in sports performance. The purpose of this study is to analyze the psychological characteristics of young soccer players and their relation to their performance perception, made both by the player herself and by their coaches. The sample is composed of 108 women (M age = 15.53, SD age = 1.05), with ages between 13 and 17 years (13 years, n = 1, 14 years, n = 18, 15 years, n = 36, 16 years, n = 29, 17 years, n = 24), and with a sport practice experience of 7.27 years on average (SD = 2.64). For to address this aim, we used the Psychological Characteristics related to the Sport Performance Questionnaire (CPRD) and the Psychological Skills Inventory for Sports (PSIS). In addition, regarding the evaluation of performance perception, an ad hoc short questionnaire was created, composed by one question addressed to the player and one directed to the coach. The results indicate that the group of players of the under-16 category obtained higher scores in all the psychological dimensions than the U-18 players, showing significant differences in Team Cohesion (p < 0.048). Regarding the degree of congruence between the player’s psychological features, and the player’s and coach’s performance perceptions, the results show statistically significant and negative correlations between the Team Cohesion factor and the athlete’s own outcome perception for the match #1 (rxy = -0.479; p < 0.001), and match #2 (rxy = -0.402; p < 0.01). The results of this study may contribute to establish the differences between different constellations of psychological characteristics according to the categories of competition and their relationship with the perception of performance. This knowledge can be used by sports professionals: coaches, psychologists, physical educators, etc., in order to help athletes to reach their maximum performance.
Limits of Perceived Audio-Visual Spatial Coherence as Defined by Reaction Time Measurements

The ventriloquism effect describes the phenomenon of audio and visual signals with common features, such as a voice and a talking face merging perceptually into one percept even if they are spatially misaligned. The boundaries of the fusion of spatially misaligned stimuli are of interest for the design of multimedia products to ensure a perceptually satisfactory product. They have mainly been studied using continuous judgment scales and forced-choice measurement methods. These results vary greatly between different studies. The current experiment aims to evaluate audio-visual fusion using reaction time (RT) measurements as an indirect method of measurement to overcome these great variances. A two-alternative forced-choice (2AFC) word recognition test was designed and tested with noise and multi-talker speech background distractors. Visual signals were presented centrally and audio signals were presented between 0° and 31° audio-visual offset in azimuth. RT data were analyzed separately for the underlying Simon effect and attentional effects. In the case of the attentional effects, three models were identified but no single model could explain the observed RTs for all participants so data were grouped and analyzed accordingly. The results show that significant differences in RTs are measured from 5° to 10° onwards for the Simon effect. The attentional effect varied at the same audio-visual offset for two out of the three defined participant groups. In contrast with the prior research, these results suggest that, even for speech signals, small audio-visual offsets influence spatial integration subconsciously.
Why Are People High in Dispositional Awe Happier? The Roles of Meaning in Life and Materialism

Awe is an intense emotional response to perceptually vast stimuli that dramatically transcend one’s ordinary reference frame and provoke a need to adjust the current mental structures. Dispositional awe reflects individual differences in the tendency to experience awe. The current study aims to examine the effect of dispositional awe on subjective well-being, with a focus on confirming the mediating role of meaning in life and materialism. A sample of 563 Chinese adults completed measures of dispositional awe, meaning in life, materialism, and subjective well-being. Correlation analysis revealed that dispositional awe, meaning in life, and materialism were all significantly correlated with subjective well-being. Structural equation modeling showed significant paths from dispositional awe to subjective well-being through both meaning in life and materialism. Bootstrap analysis also indicated that meaning in life and materialism mediated the relationship between dispositional awe and subjective well-being. These findings not only corroborate the critical role of dispositional awe in promoting subjective well-being, but also shed some light on why people high in dispositional awe are happier than those low in dispositional awe. Limitations and directions for future research were also discussed.
Aurora-A Induces Chemoresistance Through Activation of the AKT/mTOR Pathway in Endometrial Cancer

Endometrial cancer (EC) is the most common gynecological tumor all over the world, and advanced/metastatic EC remains a malignancy with poor survival outcome due to highly resistant to conventional chemotherapeutic treatment. Here, we report that Aurora-A, a serine-threonine kinase, plays a vital role in chemoresistance of EC. Aurora-A is overexpressed in EC tissues, compared with normal endometrium and Aurora-A expression is associated with decreased overall survival. Overexpression of Aurora-A in EC cell lines (Ishikawa and HEC-1B cells) promotes cell proliferation and induced paclitaxel- and cisplatin-resistance. Furthermore, Aurora-A activating AKT-mTOR pathway further induces chemoresistance in vitro, consistent with a positive correlation between Aurora-A and phosphorylated AKT/4E-BP1 expression in EC tissues. In summary, our study provides the strong evidence that Aurora-A controls the sensitivity of EC cell lines to chemotherapy via AKT/mTOR pathway, indicating that pharmacologic intervention of Aurora-A and AKT/mTOR in combination with chemotherapy may be considered for the targeted therapy against EC with overexpression of Aurora-A.
Glucose Abnormalities Associated to Prolactin Secreting Pituitary Adenomas

The pathogenesis of obesity and alterations in glucose profile have been linked to PRL excess, as it is reportedly associated with metabolic syndrome in thereabout one third of patients. In vitro exposure of pancreatic islet to PRL is known to stimulate insulin secretion and β-cell proliferation, and in turn overexpression of PRL in β-cells increases insulin release and β-cell replication. PRL excess has been found to worsen glucose profile because it reduces glucose tolerance and induces insulin resistance either in obese and non-obese patients. To note, pancreatic β-cells and adipocytes widely express dopamine receptors type 2, and dopamine has been hypothesized to play a key role as modulator of insulin and adipose functions. The dopamine agonists bromocriptine and cabergoline significantly improve abnormalities in glucose profile and reduce the prevalence of metabolic syndrome in a remarkable proportion of patients, regardless of whether body weight and PRL status may change. However, in men with hyperprolactinemia complicated by hypogonadism, testosterone replacement can ameliorate insulin resistance and abnormalities in glucose metabolism. Therefore, in patients with PRL-secreting pituitary adenomas control of PRL excess by dopamine agonists is mandatory to improve glucose and insulin abnormalities.
Ecological Drivers of Invasive Lionfish (Pterois volitans and Pterois miles) Distribution Across Mesophotic Reefs in Bermuda

Invasive lionfish (Pterois volitans and P. miles) are now ubiquitous throughout the Caribbean and Western Atlantic on shallow and deep reefs. Recent surveys in Bermuda have revealed dense aggregations of lionfish on mesophotic reefs (60 m depth), yet these densities are not pervasive across reefs at this depth. Using diver-led visual surveys of mesophotic reef sites, this study examines how variations in potential ecological drivers may affect lionfish distribution. Significant correlations of lionfish densities were found with prey fish density and prey fish biomass, where sites with higher abundances of prey fishes have greater densities of lionfish. Furthermore, higher densities of lionfish also correlated significantly with higher juvenile Paranthias furcifer biomass, a preferred prey type for lionfish. Prey fish diversity, on the other hand, was not related to lionfish density, nor did prey fish community composition differ in a way that reflected lionfish distributions. The influence of seawater temperature was found to have the strongest effect on lionfish distribution, where higher lionfish densities were found at sites with lower bottom temperature. However, temperature co-varied with prey fish density, prey fish biomass, and P. furcifer biomass, implying that physical parameters of the environment (i.e. temperature) likely influence ecological parameters (i.e. prey fish abundance), contributing to the structuring of lionfish distributions. We suggest, therefore, that cold-water upwelling currents may be fueling the food chain in certain locations, resulting in high abundances of prey fishes and thus lionfish. Understanding the factors that influence lionfish distributions will ultimately increase the efficacy of management strategies, which, as the data presented here suggest, must incorporate mesophotic lionfish populations.
When Does Work Interfere With Teachers’ Private Life? An Application of the Job Demands-Resources Model

The purpose of this study is to examine the relationship between contextual work-related factors on the one hand, in terms of job demands (i.e., risk factors) and job resources (i.e., protective factors), and work-family conflict (WFC) in teachers on the other. Building on the Job Demands-Resources (JD-R) model, we hypothesized that job demands, namely qualitative, and quantitative workload, are positively associated with WFC in teachers. Moreover, in line with the buffer hypothesis of the JD-R, we expected job resources, in terms of support from supervisor (SS), job autonomy (JA), and participation in decision making (PDM), to affect this association, which is expected to be stronger when job resources are low. The study was conducted in an Italian secondary school. Overall, 122 teachers completed a self-report questionnaire aimed at determining WFC, as well as job demands and resources. The hypothesized relationships were tested using moderated multiple regression. The results of this study largely support our predictions. First, both aspects of workload were positively associated with WFC. Secondly, job resources, including SS and PDM, buffered this association, which was stronger when resources were low. On the contrary, JA did not buffer the association between workload and WFC. Overall, the results of this study are consistent with the JD-R model and contribute to the understanding of work–family conflict among teachers. More specifically, our study suggests that teachers with high levels of job resources, namely SS and PDM, can effectively cope with job demands, in terms of both qualitative and quantitative workload, thus preventing negative consequences such as conflict between work and family domains. Interventions aimed at preventing WFC among teachers should encourage organizations to optimize the balance between job demands and resources, as well as the identification and training of the workers at risk of WFC.
Off-Target Effects of Clozapine-N-Oxide on the Chemosensory Reflex Are Masked by High Stress Levels

Respiratory chemosensory circuits are implicated in several physiological and behavioral disorders ranging from sudden infant death syndrome to panic disorder. Thus, a comprehensive map of the chemosensory network would be of significant value. To delineate chemosensory neuronal populations, we have utilized pharmacogenetic Designer Receptors Exclusively Activated by Designer Drugs (DREADD) perturbations for acute neuronal perturbations in respiratory circuit mapping. Recent studies show that the biologically inert DREADD ligand clozapine-N-oxide (CNO) is back-metabolized into the bioactive compound clozapine in rodents, emphasizing the need for CNO-only DREADD-free controls, which have been carried out in several studies. However, we show that high CNO doses used in several chemosensory circuit mapping studies nonetheless affect the chemosensory ventilatory reflexes in control mice, which is unmasked by extensive habituation. Here, unhabituated control animals showed no differences in respiratory parameters after CNO administration, whereas habituated animals receiving the commonly used dose of 10 mg/kg of CNO show a deficit in the hypercapnic (high CO2) chemosensory reflex, which is not present in 1 mg/kg CNO treated or saline control groups. Our findings indicate that even in appropriately controlled studies, additional masked CNO off-target effects may exist and underscore the importance of using minimal doses of activating ligand in combination with high levels of habituation.
Zika Virus Induced More Severe Inflammatory Response Than Dengue Virus in Chicken Embryonic Livers

Dengue (DENV) and Zika virus (ZIKV) are important flaviviruses in tropical and subtropical regions, causing severe Dengue Hemorrhagic Fever (DHF)/Dengue Shock Syndrome (DSS) and microcephaly, respectively. The infection of both viruses during pregnancy were reported with adverse fetal outcomes. To investigate the effects of ZIKV and DENV infections on fetal development, we established an infection model in chicken embryos. Compared with DENV-2, the infection of ZIKV significantly retarded the development of chicken embryos. High viral loads of both DENV-2 and ZIKV was detected in brain, eye and heart 7 and 11 days post-infection, respectively. Interestingly, only ZIKV but not DENV-2 was detected in the liver. Even both of them induced apparent liver inflammation, ZIKV infection showed a more severe inflammatory response than DENV-2 infection based on the inflammation scores and the gene expression levels of IL-1β, TNF, IL-6, and TGFβ-2 in liver. Our results demonstrated that ZIKV induced more severe inflammatory response in chicken embryo liver compared to DENV-2, which might partially attribute to viral replication in liver cells. Clinicians should be aware of the potential liver injury associated with ZIKV infection in patients, especially in perinatal fetuses.
Acid-Sensing Ion Channel 1a Is Involved in N-Methyl D-Aspartate Receptor-Dependent Long-Term Depression in the Hippocampus

Acid-sensing ion channels (ASICs), members of the degenerin/epithelial Na+ channel superfamily, are largely expressed in the mammalian nervous system. ASIC1a is highly permeable to Ca2+ and are involved in many physiological processes, including synaptic plasticity, learning, and memory. To clarify the role of ASIC1a in synaptic transmission and plasticity, we investigated N-methyl D-aspartate (NMDA) receptor-dependent long-term depression (LTD) in the CA1 region of the hippocampus. We found that: (1) ASIC1a mediates a component of ASIC1a excitatory postsynaptic currents (EPSCs); (2) ASIC1a plays a role in electrical LTD induced by LFS protocol both in P13-18 and P30-40 animals; (3) ASIC1a is involved in chemical LTD induced by brief bath application of NMDA both in P13-18 and P30-40 animals; and finally (4) a functional interaction between ASIC1a and NMDA receptors occurs during LTD. These findings suggest a new role for ASIC1a in specific forms of synaptic plasticity in the mouse hippocampus.
The Cost of Toxicity in Microalgae: Direct Evidence From the Dinoflagellate Alexandrium

Empirical evidence of the cost of producing toxic compounds in harmful microalgae is completely lacking. Yet costs are often assumed to be high, implying substantial ecological benefits with adaptive significance exist. To study potential fitness costs of toxin production, 16 strains including three species of the former Alexandrium tamarense species complex were grown under both carbon limitation and unlimited conditions. Growth rates, levels of intracellular paralytic shellfish toxins (PSTs), and effects of lytic compounds were measured to provide trade-off curves of toxicity for both PST and lytic toxicity under high light (300 μmol photons m−2 s−1) and under low light (i.e., carbon limited; 20 μmol photons m−2 s−1). Fitness costs in terms of reduced growth rates with increasing PST content were only evident under unlimited conditions, but not under carbon limitation, in which case PST production was positively correlated with growth. The cost of production of lytic compounds was detected both under carbon limitation and unlimited conditions, but only in strains producing PST. The results may direct future research in understanding the evolutionary role and ecological function of algal toxins. The intrinsic growth rate costs should be accounted for in relation to quantifying benefits such as grazer avoidance or toxin-mediated prey capture in natural food web settings.