Miracle-dz/UniFAR-FTUs huggingface dataset:
In Vivo Evidence for Voltage-Gated Sodium Channel Expression in Carcinomas and Potentiation of Metastasis

A wide body of evidence suggests that voltage-gated sodium channels (VGSCs) are expressed de novo in several human carcinomas where channel activity promotes a variety of cellular behaviours integral to the metastatic cascade. These include directional motility (including galvanotaxis), pH balance, extracellular proteolysis, and invasion. Contrary to the substantial in vitro data, however, evidence for VGSC involvement in the cancer process in vivo is limited. Here, we critically assess, for the first time, the available in vivo evidence, hierarchically from mRNA level to emerging clinical aspects, including protein-level studies, electrolyte content, animal tests, and clinical imaging. The evidence strongly suggests that different VGSC subtypes (mainly Nav1.5 and Nav1.7) are expressed de novo in human carcinoma tissues and generally parallel the situation in vitro. Consistent with this, tissue electrolyte (sodium) levels, quantified by clinical imaging, are significantly higher in cancer vs. matched non-cancer tissues. These are early events in the acquisition of metastatic potential by the cancer cells. Taken together, the multi-faceted evidence suggests that the VGSC expression has clinical (diagnostic and therapeutic) potential as a prognostic marker, as well as an anti-metastatic target. The distinct advantages offered by the VGSC include especially (1) its embryonic nature, demonstrated most clearly for the predominant neonatal Nav1.5 expression in breast and colon cancer, and (2) the specifically druggable persistent current that VGSCs develop under hypoxic conditions, as in growing tumours, which promotes invasiveness and metastasis.
Characterization and Antioxidant Activity of Essential Oil of Four Sympatric Orchid Species

The volatile fractions from fresh inflorescences of naturally growing orchids Anacamptis coriophora (L.) R. M. Bateman, Pridgeon & M. W. Chase subsp. fragrans (Pollini), Anacamptis pyramidalis (L.) R. Ophrys holosericea (Burm.) Greuter and Serapias vomeracea (Burm. f.) B. were isolated by steam distillation and analyzed by GC/FID and GC/MS. Saturated hydrocarbons were quantified as the major constituents of the volatile fraction (47.87–81.57% of the total essential oil), of which long-chain monounsaturated hydrocarbons accounted from 9.20% to 32.04% of the total essential oil. Double bond position in linear alkenes was highlighted by dimethyl disulfide derivatization and MS fragmentation. Aldehydes (from 3.45 to 18.18% of the total essential oil), alcohols (from 0.19% to 13.48%), terpenes (from 0.98 to 2.50%) and acids (0.30 to 2.57%) were also detected. These volatiles compounds may represent a particular feature of these plant species, playing a critical role in the interaction with pollinators. DPPH assay evaluating the antioxidant activity of the essential oils was carried out, showing a dose-dependent antioxidant activity.
Pitch of Harmonic Complex Tones: Rate and Temporal Coding of Envelope Repetition Rate in the Inferior Colliculus of Unanesthetized Rabbits.

Harmonic complex tones (HCT) found in speech, music and animal vocalizations evoke strong pitch percepts at their fundamental frequencies. The strongest pitches are produced by HCT that contain harmonics resolved by cochlear frequency analysis, but HCT containing solely unresolved harmonics also evoke a weaker pitch at their envelope repetition rate (ERR). In the auditory periphery, neurons phase lock to the stimulus envelope, but this temporal representation of ERR degrades and gives way to rate codes along the ascending auditory pathway. To assess the role of the inferior colliculus (IC) in such transformations, we recorded IC neuron responses to HCT and sinusoidally modulated broadband noise (SAMN) with varying ERR from unanesthetized rabbits. Different inter-harmonic phase relationships of HCT were used to manipulate the temporal envelope without changing the power spectrum. Many IC neurons demonstrated bandpass rate tuning to ERR between 60-1600 Hz for HCT, and 40-500 Hz for SAMN. The tuning was not related to the pure-tone best frequency of neurons, but was dependent on the shape of stimulus envelope, indicating a temporal, rather than spectral origin. A phenomenological model suggests the tuning may arise from peripheral temporal response patterns via synaptic inhibition. We also characterized temporal coding to ERR. Some IC neurons could phase lock to the stimulus envelope up to 900 Hz for either HCT or SAMN, but phase locking was weaker with SAMN. Together, the rate code and the temporal code represent a wide range of ERR, providing strong cues for the pitch of unresolved harmonics.
Consumer attitude towards purchasing intent for ready to drink orange juice and nectar

Purpose – The purpose of this paper was to evaluate the impact of some labeling aspects on the consumer intent to purchase ready to drink orange juice and nectar.Design/methodology/approach – The influence of label information on the consumer intent to purchase was evaluated by conjoint analysis using a convenience sample (n=149). A factorial design with four characteristics, price, brand, information about the product and kind of beverage, was used. Three levels were established for brand and product information, and two for price and kind of beverage.Findings – Low price, product information and market leading brand had positive impact. “No preservatives/natural” was the information that most influenced consumer's purchase intent. The ideal label showed the leading brand, low price and information “no preservatives/natural”. These results could be useful for strategic planning of consumer instruction and have important implications for Brazilian orange juice manufactures.Originality/value – Although the.
Time to diagnosis in Cushing's syndrome: A meta-analysis based on 5367 patients.

CONTEXT Signs and symptoms of Cushing's syndrome (CS) overlap with common diseases, such as the metabolic syndrome, obesity, osteoporosis, and depression. Therefore, it can take years to finally diagnose CS, though early diagnosis is important for prevention of complications. OBJECTIVE The aim of this study was to assess the time span between first symptoms and diagnosis of CS in different populations in order to identify factors associated with an early diagnosis. DATA SOURCES A systematic literature search via PubMed was performed to identify studies reporting on time to diagnosis in CS. In addition, unpublished data from patients of our tertiary care center and 4 other centers were included. STUDY SELECTION Clinical studies reporting on the time to diagnosis of CS were eligible. Corresponding authors were contacted to obtain additional information relevant to the research question. DATA EXTRACTION Data were extracted from the text of the retrieved articles and from additional information provided by authors contacted successfully. From initially 3326 screened studies 44 were included. DATA SYNTHESIS Mean time to diagnosis for patients with CS was 34 months (ectopic CS: 14 months; adrenal CS: 30 months; and pituitary CS: 38 months; p<0.001). No difference was found for gender, age (</≥ 18 years), and year of diagnosis (before/after 2000). Patients with pituitary CS had a longer time to diagnosis in Germany than elsewhere. CONCLUSIONS Time to diagnosis differs for subtypes of CS but not for gender and age. Time to diagnosis remains to be long and requires to be improved.
Future health providers’ willingness to provide abortion services following decriminalisation of abortion in Chile: a cross-sectional survey

Objective To assess Chilean medical and midwifery students’ attitudes and willingness to become trained to provide abortion care, shortly after abortion was decriminalised in 2017. Design We fielded a cross-sectional, web-based survey of medical and midwifery students. We used generalised estimating equations to assess differences by type of university and degree sought. Setting We recruited students from a combination of seven secular, religiously-affiliated, public and private universities that offer midwifery or medical degrees with a specialisation in obstetrics and gynaecology, located in Santiago, Chile. Participants Students seeking medical or midwifery degrees at one of seven universities were eligible to participate. We distributed the survey link to medical and midwifery students at these seven universities; 459 eligible students opened the survey link and 377 students completed the survey. Primary and secondary outcomes Intentions to become trained to provide abortion services was our primary outcome of interest. Secondary outcomes included moral views and concerns about abortion provision. Results Most students intend to become trained to provide abortion services (69%), 20% reported that they will not provide an abortion under any circumstance, half (50%) had one or more concern about abortion provision and 16% agreed/strongly agreed that providing abortions is morally wrong. Most believed that their university should train medical and midwifery students to provide abortion services (70%–79%). Secular university students reported higher intentions to provide abortion services (beta 0.47, 95% CI: 0.31 to 0.63), more favourable views (beta 0.52, CI: 0.32 to 0.72) and were less likley to report concerns about abortion provision (adjusted OR 0.47, CI: 0.23 to 0.95) than students from religious universities. Conclusion Medical and midwifery students are interested in becoming trained to provide abortion services and believe their university should provide this training. Integrating high-quality training in abortion care into medical and midwifery programmes will be critical to ensuring that women receive timely, non-judgemental and quality abortion care.
Examining effects of parent warmth and control on internalizing behavior clusters from age 8 to 12 in 12 cultural groups in nine countries.

BACKGROUND Studies of U.S. and European samples demonstrate that parental warmth and behavioral control predict child internalizing behaviors and vice versa. However, these patterns have not been researched in other cultures. This study investigates associations between parent warmth and control and three child-reported internalizing behavior clusters to examine this question. METHODS Data from 12 cultural groups in 9 countries were used to investigate prospective bidirectional associations between parental warmth and control, and three child-reported internalizing behavior types: withdrawn/depressed, anxious/depressed, and somatic problems. Multiple-group structural equation modeling was used to analyze associations in children followed from ages 8 to 12. RESULTS Parent warmth and control effects were most pervasive on child-reported withdrawn/depressed problems, somewhat pervasive on anxious/depressed problems and least pervasive on somatic problems. Additionally, parental warmth, as opposed to control, was more consistently associated with child-reported internalizing problems across behavior clusters. Child internalizing behavior effects on parental warmth and control appeared ubiquitously across cultures, and behaviors, but were limited to ages 8-10. Most effects were pancultural, but culture-specific effects emerged at ages 9-10 involving the associations between parent warmth and withdrawn/depressed and somatic behaviors. CONCLUSIONS Effects of parent warmth and control appear stronger on some types of child-reported internalizing behaviors. Associations are especially strong with regard to parental warmth across cultures, and culture-specific effects may be accounted for by cultural normativeness of parent warmth and child-reported somatic symptoms. Child internalizing behavior effects on subsequent parenting are common across cultures.
Self-renewing endometrial epithelial organoids of the human uterus

Significance The inner lining of the human uterus, termed the endometrium, is replete with glands that are hypothesized to be important for establishment of pregnancy through interactions with other endometrial cells and the embryo. A 3-dimensional culture system was utilized to establish and generate endometrial epithelial organoids from normal human endometrium that display long-term expandability, can be cryopreserved, and are hormone responsive. Here, single-cell RNA-sequencing was used to create a high-resolution gene expression atlas of the endometrial epithelial organoids and to define their responsiveness to the reproductive hormones estrogen and progesterone. This work is important to develop platforms to study the physiology and pathology of the human endometrium. The human endometrium is essential in providing the site for implantation and maintaining the growth and survival of the conceptus. An unreceptive endometrium and disrupted maternal−conceptus interactions can cause infertility due to pregnancy loss or later pregnancy complications. Despite this, the role of uterine glands in first trimester human pregnancy is little understood. An established organoid protocol was used to generate and comprehensively analyze 3-dimensional endometrial epithelial organoid (EEO) cultures from human endometrial biopsies. The derived EEO expand long-term, are genetically stable, and can be cryopreserved. Using endometrium from 2 different donors, EEO were derived and then treated with estrogen (E2) for 2 d or E2 and medroxyprogesterone acetate (MPA) for 6 d. EEO cells were positive for the gland marker, FOXA2, and exhibited appropriate hormonal regulation of steroid hormone receptor expression. Real-time qPCR and bulk RNA-sequencing analysis revealed effects of hormone treatment on gene expression that recapitulated changes in proliferative and secretory phase endometrium. Single-cell RNA sequencing analysis revealed that several different epithelial cell types are present in the EEO whose proportion and gene expression changed with hormone treatment. The EEO model serves as an important platform for studying the physiology and pathology of the human endometrium.
The role of metacognition and its indirect effect through cognitive attentional syndrome on fear of cancer recurrence trajectories: A longitudinal study

This longitudinal study mapped distinct trajectories of fear of cancer recurrence (FCR) over 12 months among patients with breast (BC) or colorectal (CRC) cancer, and examined if metacognition, indirectly via attentional bias, intrusive thoughts and avoidance (hallmarks of cognitive attentional syndrome; CAS) predicted FCR trajectory membership.
The Clinical Utility of the Chen Internet Addiction Scale—Gaming Version, for Internet Gaming Disorder in the DSM-5 among Young Adults

Objectives: The Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5) includes the diagnostic criteria for Internet gaming disorder (IGD). This study evaluated (1) the screening, diagnostic, and prevalence-estimated cutoff points of the Chen Internet Addiction Scale–Gaming Version (CIAS-G) for IGD in the DSM-5; and (2) the differences in the CIAS-G and subscale scores among individuals with IGD, regular gamers (RGs), and other control subjects. Methods: We recruited 69 participants with IGD, 69 RGs, and 69 healthy participants based on diagnostic interviews conducted by a psychiatrist according to DSM-5 IGD criteria. All participants completed the CIAS-G and were assessed using the clinical global impression scale. Results: The optimal screening and diagnostic cutoff points were 68 or more (sensitivity, 97.1%; specificity, 76.8%) and 72 or more (sensitivity, 85.5%; specificity, 87.0%) for IGD based on DSM-5 criteria, respectively. The 76 or more cutoff point had the highest number needed to misdiagnose and was the optimal prevalence estimated cutoff point. Conclusions: The screening cutoff point could be used to identify individuals with IGD for further diagnostic interviewing to confirm the diagnosis in the clinical setting or for two-stage epidemiological evaluation. The diagnostic cutoff point provides a provisional diagnosis of IGD when diagnostic interviewing is unavailable. The prevalence-estimated cutoff point could be used to estimate the prevalence of IGD in large-scale epidemiological investigations when further diagnostic interviewing is impractical. The clinical and epidemiological utility of CIAS-G warrants further study.
Genetic Association and Linkage Studies in Osteoarthritis

Annu Nakki1,2,3, Minna Mannikko4 and Janna Saarela1 1Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, 2Unit of Public Health Genomics, National Institute for Health and Welfare, Helsinki, 3Departments of Medical Genetics and Public Health, University of Helsinki, Helsinki, 4Oulu Center for cell-Matrix Research, Biocenter and Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland
Prospective 1-year follow-up study of all cured, new sputum smear positive tuberculosis patients under the Revised National Tuberculosis Control Program in Hyderabad, Telangana State, India

Background: Tuberculosis (TB) is one of the leading causes of mortality in India. The Revised National Tuberculosis Control Program (RNTCP) is a robust public health system to deal with TB in India. Unless the treated patient comes back to the system with signs and symptoms of TB due to relapse or re-infection, there is no mechanism of follow-up or any method to know the relapse rate in the population. We attempted to follow the patients declared as “Cured” as per the RNTCP guidelines for 1–2 years to identify the health status of the index cases and their household contacts in posttreatment phase. Materials and Methods: In this prospective cohort study, 187 index cases, who were declared “Cured” in six randomly selected TB units of Hyderabad district, were followed up for 1–2 years through home visits by trained staff with structured data collection forms. Data were analyzed using SPSS v20.0. Results: The mean age of the index cases was 33.64 (±16.10) years, and there were 75 females and 112 males. The study sample was homogenous for gender, age, smear grade, religion, marital status, smoking status, alcohol consumption, and human immunodeficiency virus status, etc., At 1-year posttreatment follow-up of 187 index cases, 143 (76.47%) were healthy and working without any symptoms of TB. Symptoms of TB were present in 26 (13.90%) cases, and seven index cases (4.06%) were re-diagnosed with TB. The 2-year posttreatment survival was 92%. Conclusion: Long-term follow-up of cured, new smear-positive TB cases reinforce the effectiveness of anti-TB treatment under the RNTCP as assessed by improved health outcomes in more than two-thirds of cases and posttreatment survival of 92% of index cases. We recommend continuing such follow-up for all TB cases treated under the RNTCP for effective end-TB strategy.
A relational perspective on women’s empowerment: Intimate partner violence and empowerment among women entrepreneurs in Vietnam

Research has mainly studied women’s empowerment assessing personal (e.g., self‐esteem) or relational (e.g., decision‐making) empowerment indicators. Women are not isolated individuals; they are embedded in social relationships. This is especially relevant in more collectivist societies. The current research provides a relational perspective on how husbands may hamper women’s empowerment by inflicting intimate partner violence (IPV) assessing women’s self‐reported experience. We tested the link between self‐esteem and experienced IPV on financial intra‐household decision‐making power among women entrepreneurs (N = 1,347) in Northern Vietnam, a collectivistic society undergoing economic development. We report two measurement points. As expected, self‐esteem (and not IPV) was positively related to more power in intra‐household decision‐making on small expenditures, which are traditionally taken by women. However, IPV (and not self‐esteem) was related to less decision‐making power on larger expenditures, traditionally a domain outside women’s power. We test and discuss the directionality of the effects and stress the importance of considering women’s close relationship when investigating signs of women’s empowerment.
Elderly medicine: a training guide

This is an excellent and concise book that divides the complex area of falls into bite-size chunks. The book takes the reader through the basic science of falls in older people and describes the assessment, management and prevention of falls. Each chapter is designed to stand alone and covers a single topic. The writing is clear and can be read by a general clinical audience without being simplistic or patronising. There is a good balance between evidence, description and clinical scenario, and the book manages to be factual without being overly dogmatic. The book as a whole spans the subject with appropriate breadth and depth for all but the most advanced or specialised practitioner. The book is mainly geared towards falls nurse specialists and allied health professionals but should be of interest to trainees, general practitioners and consultants. Anyone looking to prepare a teaching programme on falls, for patients or staff, would be well advised to use this book as a starting point. In short, this book lives up to its title.
HUBUNGAN SKOR FREKUENSI DIET BEBAS GLUTEN BEBAS CASEIN DENGAN SKOR PERILAKU AUTIS

Latar Belakang: Salah satu upaya untuk mengurangi terjadinya perilaku autis, yaitu dengan cara menghindari makanan yang mengandung gluten maupun casein sebab pada penderita autis terjadi hipermeabilitas mukosa usus yang mengakibatkan kedua jenis protein tersebut sulit dicerna dan akan membentuk suatu zat yang disebut peptide. Peptide gluten menghasilkan gluteomorphin atau gliadimorphin dan peptide casein membentuk caseomorphin. Kedua zat tersebut dapat mempengaruhi sistem saraf pusat sehingga menimbulkan gangguan perilaku. Metode: Penelitian o bservasional dengan desain cross sectional yang dilakukan di Pusat Terapi Pendidikan Ananda Bekasi. Jumlah sampel sebanyak 30 subjek yang dipilih menggunakan metode purposive sampling. Data yang dikumpulkan , meliputi data identitas sampel berupa usia terdiagnosis autis, berat badan dan tinggi badan, berat badan saat lahir, data karakteristik orangtua, usia ibu saat hamil subjek, data skor frekuensi diet bebas gluten bebas casein diperoleh melalui FFQ serta data pengamatan perilaku yang diperoleh melalui kuesioner lembar perilaku ICD-10. Analisis bivariat dilakukan dengan uji korelasi Pearson Product Moment. Hasil: Penderita autis lebih banyak ditemukan pada laki-laki (83,3%) dibandingkan perempuan. Usia terdiagnosis autis paling dominan antara 1-2 tahun (53,3%) dan sebagian besar status gizinya normal (46,7%) , tetapi adapula subjek (40%) yang mengalami overweight dan obesitas. Subjek yang mengalami penurunan perilaku autis sebesar (26,7%). Seluruh (100%) subjek pada penelitian ini masih mengonsumsi makanan yang mengandung gluten maupun casein. H asil uji bivariat menunjukkan ada hubungan antara skor frekuensi diet bebas gluten bebas casein dengan skor perilaku autis (r=0.369, p=0.045). Simpulan: Terdapat hubungan antara skor frekuensi diet bebas gluten bebas casein dengan skor perilaku autis.