Miracle-dz/UniFAR-FTUs huggingface dataset:
Causes and Consequences of Risk Aversion in Middle Adulthood

According to the Census Bureau, the median asset value of people over 65 is over $170,000, and decisions about investments that led up to possessing these assets would have been made prior to retirement, during prime working age. In this paper, I examine some determinants of those decisions. We know that risk aversion plays a role in stochastic decision-making. However, I expand on that knowledge by analyzing how risk aversion may affect decision-making over time, what effects risk aversion may have on financial and health decisions, and whether one’s level of risk aversion varies over the lifetime, using the Health and Retirement Study dataset. I find that risk aversion is correlated with certain maternal factors, income, depression, and ethnicity. Risk aversion, then, is correlated with decisions about financial investments and the likelihood of partaking in certain risky behaviors. The tendency to be variable in risk aversion preferences appears divided based on internal factors versus external circumstances.
RGS7 and RGS8 Differentially Accelerate G Protein-mediated Modulation of K+ Currents*

The recently discovered family of RGS (regulators of G protein signaling) proteins acts as GTPase activating proteins which bind to α subunits of heterotrimeric G proteins. We previously showed that a brain-specific RGS, RGS8 speeds up the activation and deactivation kinetics of the G protein-coupled inward rectifier K+ channel (GIRK) upon receptor stimulation (Saitoh, O., Kubo, Y., Miyatani, Y., Asano, T., and Nakata, H. (1997)Nature 390, 525–529). Here we report the isolation of a full-length rat cDNA of another brain-specific RGS, RGS7. In situ hybridization study revealed that RGS7 mRNA is predominantly expressed in Golgi cells within granule cell layer of cerebellar cortex. We observed that RGS7 recombinant protein binds preferentially to Gαo, Gαi3, and Gαz. When co-expressed with GIRK1/2 inXenopus oocytes, RGS7 and RGS8 differentially accelerate G protein-mediated modulation of GIRK. RGS7 clearly accelerated activation of GIRK current similarly with RGS8 but the acceleration effect of deactivation was significantly weaker than that of RGS8. These acceleration properties of RGS proteins may play important roles in the rapid regulation of neuronal excitability and the cellular responses to short-lived stimulations.
Dietary fish oil decreases secretion of T helper (Th) 1-type cytokines by a direct effect on murine splenic T cells but enhances secretion of a Th2-type cytokine by an effect on accessory cells

Dietary fish oil is considered to have anti-inflammatory effects based primarily on its effects on T-cell proliferation and IL-2 secretion. Its effects on the secretion of T helper (Th) 1-type cytokines vary and few studies have examined its effects on the secretion of Th2-type cytokines. In the present study, we examined the effects of dietary fish oil on the secretion of Th1 and Th2-type cytokines by splenocytes and the mechanism by which dietary fish oil affects Th2-type cytokine secretion. Mice were fed diets supplemented with 18 % fish oil (w/w) +2 % maize oil or 20 % maize oil for 6 weeks. Spleen cells, isolated splenic T cells and accessory cells (splenocytes depleted of T cells) were stimulated with anti-CD3/anti-CD28. The secretion of interferon (IFN)-γ, TNF-α, IL-4 and IL-10 was measured by ELISA. Dietary fish oil decreased the secretion of IFN-γ and TNF-α by total splenocytes and isolated T cells. In contrast, dietary fish oil increased the secretion of IL-4 by total splenocytes but had no effect on IL-4 secretion by isolated T cells. When isolated T cells were cultured with CD11b+ cells (mainly macrophages), cells from mice fed the fish oil diet secreted more IL-4 than cells from mice fed the maize oil diet. These results demonstrate that dietary fish oil directs cytokine secretion by splenocytes towards a Th2 phenotype and that the effects of dietary fish oil on the secretion of a Th2-type cytokine are mediated by its effect on CD11b+ accessory cells.
A Helper-Dependent Capsid-Modified Adenovirus Vector Expressing Adeno-Associated Virus Rep78 Mediates Site-Specific Integration of a 27-Kilobase Transgene Cassette

ABSTRACT Random integration of viral gene therapy vectors and subsequent activation or disruption of cellular genes poses safety risks. Major efforts in the field are aimed toward targeting vector integration to specific sites in the host genome. The adeno-associated virus (AAV) Rep78 protein is able to target AAV integration to a specific site on human chromosome 19, called AAVS1. We studied whether this ability could be harnessed to achieve site-specific integration of a 27-kb transgene cassette into a model cell line for human hematopoietic cells (Mo7e). To deliver rep78 and the transgene to Mo7e cells, we used helper-dependent adenovirus (Ad) vectors containing Ad serotype 35 fiber knob domains (HD-Ad). An HD-Ad vector containing the rep78 gene under the control of the globin locus control region (LCR) (Ad.LCR-rep78) conferred Rep78 expression on Mo7e cells. Upon coinfection of Ad.LCR-rep78 with an HD-Ad vector containing a 27-kb globin-LCR-green fluorescent protein (GFP) transgene cassette flanked by AAV inverted terminal repeats (ITRs) (Ad.AAV-LCR-GFP), transduced cells were cloned and expanded (without selection pressure), and vector integration was analyzed in clones with more than 30% GFP-positive cells. Vector integration into the AAVS1 region was seen in 30% of analyzed integration sites, and GFP expression from these integrants was stable over time. Of the remaining integration sites, 25% were within the genomic globin LCR. In almost 90% of sites, transgene integration occurred via the Ad ITR. This indicates that rescue of the AAV ITR-flanked transgene cassette from Ad.AAV-LCR-GFP is not required for Rep78-mediated integration into AAVS1 and that free ends within the vector genome can be created by breaks within the Ad ITRs, whose structure is apparently recognized by cellular “nicking” enzymes. The finding that 55% of all analyzed integration sites were either within the AAVS1 or globin LCR region demonstrates that a high frequency of targeted integration of a large transgene cassette can be achieved in human hematopoietic stem cell lines.
Influence of culture conditions on laccase production and isozyme patterns in the white‐rot fungus Trametes gallica

Laccase production by the white‐rot fungus Trametes gallica was studied, using twelve different media under static or shaking condition. The results indicated that organic nitrogen sources such as tryptone and peptone strongly improved laccase production. The application of an amino acid mixture and a lignin preparation also increased the formation of laccase, which was not observed in the presence of potato extract. Native polyacryl amide gel electrophoresis (PAGE) followed by laccase activity staining using guaiacol as the substrate was performed to analyze the laccase isozyme patterns under the different culture conditions employed. Zymograms revealed a total of twenty different laccase activity bands that appeared in individual patterns, dependent on the respective culture condition applied. This indicates that both the medium composition and the mode of incubation (static or shaking) influenced the laccase isozyme gene expression. This was the first time to report so many laccase isozymes in a fungus. Native PAGE with silver staining showed that laccases were the main protein productions in several media providing a potentially convenient way in purifying laccases from T. gallica. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Observations on a Cohort of HIV-Infected Patients Undergoing Native Renal Biopsy

Aims: This study aims to explore the spectrum of renal disease in HIV-infected patients, identify clinical predictors of HIV-associated nephropathy (HIVAN), and investigate the performance of renal biopsy in HIV-infected patients. Method: Of 263 HIV-infected patients with renal disease evaluated between 1995 and 2004, 152 had a renal biopsy, while 111 had not. A group comparison was performed. Results: The leading biopsy diagnoses were HIVAN (35%), noncollapsing focal segmental glomerulosclerosis (22%), and acute interstitial nephritis (7.9%), amongst over a dozen others. There was a trend of decreasing yearly incidence of HIVAN diagnoses, paralleling the use of antiretroviral therapy. By multivariate logistic regression, CD4 counts >200 cells/mm3 and higher estimated glomerular filtration rate were strong negative predictors of HIVAN. HIVAN patients were more likely to require dialysis (p < 0.0001) and had worse overall survival (p = 0.02). Younger age and lower estimated glomerular filtration rate were significant predictors of renal biopsy in multivariate regression analysis. More biopsied patients progressed to dialysis (51 vs. 25%, p = 0.001) and death (15 vs. 5.4%, p = 0.001), despite more frequent corticosteroid treatment (29 vs. 3.6%, p = 0.001). Conclusion: These findings may reflect more severe acute and/or chronic disease at the time of biopsy and suggests that earlier renal biopsy may be warranted in HIV-infected patients, especially in light of the changing spectrum of renal disease in this group.
Rate and amplification of drug resistance among previously-treated patients with tuberculosis in Kampala, Uganda.

BACKGROUND Drug-resistant Mycobacterium tuberculosis has emerged as a global threat. In resource-constrained settings, patients with a history of tuberculosis (TB) treatment may have drug-resistant disease and may experience poor outcomes. There is a need to measure the extent of and risk factors for drug resistance in such patients. METHODS From July 2003 through November 2006, we enrolled 410 previously treated patients with TB in Kampala, Uganda. We measured the prevalence of resistance to first- and second-line drugs and analyzed risk factors associated with baseline and acquired drug resistance. RESULTS The prevalence of multidrug-resistant TB was 12.7% (95% confidence interval [95% CI], 9.6%-16.3%). Resistance to second-line drugs was low. Factors associated with multidrug-resistant TB at enrollment included a history of treatment failure (odds ratio, 23.6; 95% CI, 7.7-72.4), multiple previous TB episodes (odds ratio, 15.6; 95% CI, 5.0-49.1), and cavities present on chest radiograph (odds ratio, 5.9; 95% CI, 1.2-29.5). Among a cohort of 250 patients, 5.2% (95% CI, 2.8%-8.7%) were infected with M. tuberculosis that developed additional drug resistance. Amplification of drug resistance was associated with existing drug resistance at baseline (P < .01) and delayed sputum culture conversion (P < .01). CONCLUSIONS The burden of drug resistance in previously treated patients with TB in Uganda is sizeable, and the risk of generating additional drug resistance is significant. There is an urgent need to improve the treatment for such patients in low-income countries.
Performance analysis of downlink OFDMA resource allocation with limited feedback

Opportunistic resource allocation in orthogonal frequency division multiple access (OFDMA) with limited feedback improves the performance of wireless networks and it has been considered in several studies. However, the performances of reduced feedback schemes have not been characterized analytically. In this paper, the analysis of the resource allocation in downlink OFDMA with limited feedback is presented. OFDMA system with a practical resource fair round robin allocation and erroneous feedback channel is considered. The performances of the resource allocation based on three feedback methods are analyzed and compared. A resource-wise signal-to-noise ratio (SNR) quantization based feedback method is considered and two feedback strategies are based on the best-M method, where the M best resource blocks are informed to the transmitter. Analytical bit error probability (BEP) expressions are derived for binary phase-shift keying (BPSK) modulation for each feedback strategy. Furthermore, an asymptotic BEP performance for large SNR values is derived for the best-M feedback schemes. The outage capacity results are derived for the considered feedback schemes to illustrate performance with slow link adaptation. Numerical results based on the analysis are compared to those obtained by computer simulations to validate the correctness of the proposed analysis.
Tailoring Education to Perceived Fall Risk in Hospitalized Patients With Cancer: A Randomized, Controlled Trial.

BACKGROUND Patients with cancer carry a higher risk for falls, potentially resulting in increased morbidity, mortality, and financial costs, as well as lower quality of life. Few evidence-based interventions are tailored to the patient's perception of risk for falls. OBJECTIVES This study aimed to determine the effect of tailored, nurse-delivered interventions as compared to a control group on patient perception of risk for falls, confidence in fall prevention, and willingness to ask for assistance. METHODS A two-group, prospective, randomized, controlled design was used to test the intervention in a convenience sample of 91 patients on an adult bone marrow transplantation unit. The intervention consisted of video and printed education tailored to the nurse's risk assessment and the patient's perception of risk. Patient's self-reported perception, confidence, and willingness were measured at three time points. FINDINGS About one-third of patients perceived themselves to be at low risk for falls despite a nurse rating of high risk. A statistically significant difference existed in the proportion of patients who perceived themselves to be at high risk for falls pre- and postintervention (p = 0.01). Results suggest that tailoring education to the patients' perceived risk for falls can help patients become more aware of fall risk.
Epidemiologic survey of accidental dentofacial injuries among U.S. Army personnel.

This epidemiologic study of accidental dentofacial injuries to U.S. Army personnel was conducted to determine the frequency and distribution patterns of accidental dentofacial injuries to soldiers. Administratively, it was anticipated that this data would permit identification of high-risk groups and would suggest feasible preventive measures. This 9-month study was conducted on 16 Army posts with a combined population at risk of 210,500 soldiers; a standardized data collection form was completed by the dental corps officer treating the injury case and then was mailed to a central collection site for analysis. The data from this dentofacial injury study clearly reveal that differential risks exist for various military subpopulations. While the overall U.S. Army accidental dentofacial injury rate was 37.7 cases/10,000/year, this rate varied greatly for specific subgroups with high-risk factors including young males, lower enlisted ranks, recent recruits, and combat training posts. The primary specific causes of these injuries were fistfights (nearly 30%), sports (over 20%), and vehicles (about 15%).
Dynamics of Fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants

In characterizing mutants and covalently inhibited complexes of Fusarium solani cutinase, which is a 197‐residue lipolytic enzyme, 34 variant structures, crystallizing in 8 different crystal forms, have been determined, mostly at high resolution. Taking advantage of this considerable body of information, a structural comparative analysis was carried out to investigate the dynamics of cutinase. Surface loops were identified as the major flexible protein regions, particularly those forming the active‐site groove, whereas the elements constituting the protein scaffold were found to retain the same conformation in all the cutinase variants studied. Flexibility turned out to be correlated with thermal motion. With a given crystal packing environment, a high flexibility turned out to be correlated with a low involvement in crystal packing contacts. The high degree of crystal polymorphism, which allowed different conformations with similar energy to be detected, made it possible to identify motions which would have remained unidentified if only a single crystal form had been available. Fairly good agreement was found to exist between the data obtained from the structural comparison and those from a molecular dynamics (MD) simulation carried out on the native enzyme. The crystallographic approach used in this study turned out to be a suitable tool for investigating cutinase dynamics. Because of the availability of a set of closely related proteins in different crystal environments, the intrinsic drawback of a crystallographic approach was bypassed. By combining several static pictures, the dynamics of the protein could be monitored much more realistically than what can be achieved on the basis of static pictures alone. Proteins 26:442–458 © 1996 Wiley‐Liss, Inc.
Development of Amygdaloid Kindling in Histidine Decarboxylase–deficient and Histamine H1 Receptor–deficient Mice

Summary: Purpose: This study attempted to clarify the role of histamine or histamine H1 receptors in the development of amygdaloid kindling by using histidine decarboxylase (HDC)‐deficient and histamine H1 receptor (H1R)‐deficient mice.
Establishing the development mechanism of ERP report

Although many scholars and executives have investigated in the related issue of enterprise resource planning (ERP), executives need to understand the operation performance of each department and the whole enterprise. Thus, various customized ERP reports are so required. However, the standard reports provided by ERP vendors fail to meet the demand of each enterprise because industries' scopes are quite diverse. So, customized report is not just an obstacle for enterprise, but also a stressful issue for ERP vendors. Therefore, this study reviews numerous research results about action research and collaborative design, then applies qualitative research method to And critical implementation items and quality control forms so to establish the ERP report design mechanism. This result has pioneered ERP report mechanism in academia. It continually improves report quality and dramatically raises overall ERP implementation value in practice.
Clinical features of infants with primary human herpesvirus 6 infection (exanthem subitum, roseola infantum).

OBJECTIVE To clarify clinical features of patients with primary human herpesvirus 6 (HHV-6) infection (roseola infantum, exanthem subitum) in a large-scale study. SUBJECTS AND METHODS Clinical signs and symptoms were analyzed in 176 infants in whom exanthem subitum was initially suspected and primary HHV-6 infection was later confirmed. The infection was proved by isolation of the virus from blood, a significant increase in the neutralizing antibody titers to the virus, or both. RESULTS The primary HHV-6 infection, which occurred throughout the year, was observed in 94 boys and 82 girls (mean age, 7.3 months). Fever developed in 98% (mean maximum fever, 39.4 degrees C) and lasted for 4.1 days. Macular or papular rashes appeared in 98%, on face, trunk, or both, mostly at the time of subsidence of the fever, and lasted for 3.8 days. Other clinical manifestations occurred as follows: mild diarrhea in 68%, edematous eyelids in 30%, erythematous papules in the pharynx in 65%, cough in 50%, and mild cervical lymph node swelling in 31%. Twenty-six percent had bulging of the anterior fontanelle and 8% had convulsions. CONCLUSIONS Clinical features of patients with virologically confirmed exanthem subitum were comparable with those described before discovery of HHV-6.
Recording sexual behavior: comparison of recall questionnaires with a coital diary.

OBJECTIVE To compare sexual behavior data obtained using a weekly-recall questionnaire, a daily-recall questionnaire, and a coital diary. DESIGN Cross-sectional survey of female sex workers from KwaZulu-Natal, South Africa between August and October 1998. METHODS In this study, 52 weekly-recall questionnaires, 27 daily-recall questionnaires, and 79 coital diaries for dates corresponding to the questionnaires were obtained from female sex workers. Variables examined included: number of clients, number of condoms used with clients and partners, and number and type of sexual acts with clients and partners. Statistical analyses were conducted to examine the degree of agreement between the data collection methods and to assess differences between the mean values of the variables in the questionnaires versus the diary. RESULTS Comparison of weekly-recall questionnaires with coital diaries indicated a significantly greater mean number of clients (P < 0.001), number of condoms used (P < 0.001), vaginal acts (P < 0.001), and anal acts (P = 0.044) reported in the diary versus the questionnaire. On comparison of daily-recall questionnaire with coital diary, significant differences were revealed between the means detected for the number of clients (P = 0.027), number of days worked (0.009), and anal acts with clients (P = 0.004). CONCLUSIONS The use of coital diaries for the collection of sexual behavior data may be limited to cross-sectional community surveys. A recall questionnaire may provide more reliable and a better quality of data for longitudinal studies and for human immunodeficiency virus/sexually transmitted disease evaluation programs.
Informed Trading in the Index Option Market

We propose a structural vector autoregressive (VAR) model of informed trading in option markets to analyze whether investors use options to trade on private information about the underlying price and/or the underlying's volatility. We decompose option order flow into exposures to the underlying asset (through the option delta) and its volatility (through the option vega). Our proposed methodological framework facilitates meaningfully aggregation of option order flows for different strike prices and maturities, and increases statistical power to identify informed trading. A fitted model confirms that S&P500 option trades are indeed informative about changes in both the underlying and volatility.
Predicting Economic Recessions Using Machine Learning Algorithms

Even at the beginning of 2008, the economic recession of 2008/09 was not being predicted. The failure to predict recessions is a persistent theme in economic forecasting. The Survey of Professional Forecasters (SPF) provides data on predictions made for the growth of total output, GDP, in the United States for one, two, three and four quarters ahead since the end of the 1960s. Over a three quarters ahead horizon, the mean prediction made for GDP growth has never been negative over this period. The correlation between the mean SPF three quarters ahead forecast and the data is very low, and over the most recent 25 years is not significantly different from zero. Here, we show that the machine learning technique of random forests has the potential to give early warning of recessions. We use a small set of explanatory variables from financial markets which would have been available to a forecaster at the time of making the forecast. We train the algorithm over the 1970Q2-1990Q1 period, and make predictions one, three and six quarters ahead. We then re-train over 1970Q2-1990Q2 and make a further set of predictions, and so on. We did not attempt any optimisation of predictions, using only the default input parameters to the algorithm we downloaded in the package R. We compare the predictions made from 1990 to the present with the actual data. One quarter ahead, the algorithm is not able to improve on the SPF predictions. Three and six quarters ahead, the correlations between actual and predicted are low, but they are very significantly different from zero. Although the timing is slightly wrong, a serious downturn in the first half of 2009 could have been predicted six quarters ahead in late 2007. The algorithm never predicts a recession when one did not occur. We obtain even stronger results with random forest machine learning techniques in the case of the United Kingdom.