Miracle-dz/UniFAR-FTUs huggingface dataset:
Coping styles, health status and advance care planning in patients with hematologic malignancies

Abstract This study evaluated whether measures of psychological well-being, including coping style, are associated with advance care planning (ACP). Data were from the Hematology Communications Study (HEMA-COMM), a prospective observational study of physician–patient communication in patients with hematologic malignancies. ACP was defined as having a living will, having a health care proxy, discussing life support with family or friends and discussing life support with a doctor or nurse. A total of 293 patients participated: only 45 (15%) had all the elements of ACP; 215 (73%) had at least one element of ACP, while 33 (11%) did not engage in ACP. In multivariate analysis, specific coping styles but not other measures of psychosocial well-being were associated with having written ACP. Verbal ACP was associated with patient-reported health and physician estimate of life expectancy. Our study suggests that tailoring ACP discussions to a patient's coping style may increase engagement in ACP.
High-resolution computer display of portable, digital, chest radiographs of adults: suitability for primary interpretation.

OBJECTIVE The suitability of using a high-resolution computer-display system for primary interpretation of adult chest radiographs obtained with portable apparatus and storage phosphor technology was evaluated with receiver operating characteristic analysis and subjective methods. MATERIALS AND METHODS Sixty portable digital chest radiographs showing abnormalities that approached the resolution limits of our computed radiography system were selected. The original digital data making up the 2140 x 1760 x 10 bit images were transferred to a cathode ray tube (CRT) display system composed of two Megascan monitors. Postprocessing rendered two images, one for each monitor, to emulate the two-on-one hard-copy format produced by the computed radiography system. Each image set was interpreted independently by three radiologists experienced with hard-copy format. Limited level and window adjustments were allowed during CRT interpretation. For both CRT and hard-copy display, images were graded on the basis of the interpreter's confidence in the presence of pneumothorax and parenchymal abnormalities. Three types of foreign devices were subjectively analyzed: endotracheal tubes, nasogastric tubes, and temporary epicardial pacer wires. RESULTS No significant differences were found in detecting pneumothorax or focal infiltrates. One radiologist found the hard copy better for detecting diffuse infiltrates (p = .02); two radiologists favored CRT for visualizing nasogastric tubes (p < .005, p < .02); and one radiologist favored CRT for visualizing temporary epicardial pacer wires (p = .05). CONCLUSION We conclude that an optimized high-resolution CRT system is quite promising for primary interpretation of digital portable chest radiographs, but further investigation and greater statistical power are necessary to confirm our results.
Electron energy loss near edge structure on the nitrogen K‐edge in vanadium nitrides

This paper presents electron energy‐loss near‐edge structure (ELNES) data for the N K edges of vanadium nitrides. By rapid thermal processing of vanadium layers in pure nitrogen at high temperatures the two known vanadium nitrides, VN and V2N, have been prepared. The phases have been checked by electron diffraction and quantitative electron energy‐loss spectroscopy (EELS) analysis. Because their crystallographical structures are different, they also exhibit different ELNES features, which can be used as fingerprints for rapidly distinguishing between VN and V2N. The experimental findings are supported by modelling the N K edge using a band structure approach (full linearized augmented plane wave method).
Energy coupling in the active transport of amino acids by bacteriohodopsin-containing cells of Halobacterium holobium

Growth of Halobacterium halobium under illumination with limiting aeration induces bacteriorhodopsin formation and renders the cells capable of photophosphorylation. Cells depleted of endogenous reserves by a starvation treatment were used to investigate the means by which energy is coupled to the active transport of [14C]proline, -leucine, and -histidine. Proline was readily accumulated by irradiated cells under anaerobiosis even when the photophosphorylation was abolished by the adenosine triphosphatase inhibitor N,N'-dicyclohexylcarbodimiide (DCCD). The uptake of proline in the dark was limited except when the cells were allowed to accumulate adenosine 5'-triphosphate (ATP) by prior light exposure or by the oxidation of glycerol. DCCD inhibited this dark uptake. These findings essentially support Mitchell's chemiosmotic theory of active transport. The driving force is apparently the proton-motive force developed when protons are extruded from irradiated bacteriorhodopsin or by the dydrolysis of ATP by membrane adenosine triphosphatase. Carbonylcyanide m-chlorophenylhydrazone (CCCP), a proton permeant known to abolish membrane potential, was a strong inhibitor of proline uptake. Leucine transport was also apparently driven by proton-motive force, although its kinetic properties differed from the proline system. Histidine transport is apparently not a chemiosmotic system. Dark- or light-exposed cells show comparable initial rats of histidine uptake, and these processes were only partially inhibited by DCCD or CCCP. The histidine system apparently does not utilize ATP per se since comparable rates of uptake were exhibited by cells of differing intracellular ATP levels. Irradiated cells did effect a greater total accumulation of histidine than dark-exposed cells. These findings suggest that ATP is needed for sustained transport.
PROBLEMS OF PHASING: A RECONSIDERATION OF THE BLACK PATCH MIDDLE BRONZE AGE ‘NUCLEATED VILLAGE’

Summary The Middle Bronze Age settlement complex of Black Patch, East Sussex, originally considered to represent the remains of a single phase ‘nucleated village’, is here reconsidered as representing the remains of two chronologically distinct settlement units. The dangers inherent in overlooking sequence and assuming contemporaneity for all archaeological features revealed within the course of a single excavation, are outlined.
SYMPOSIUM REVIEW: The role of the KATP channel in glucose homeostasis in health and disease: more than meets the islet

ATP‐sensitive potassium (KATP) channels are critical for the maintenance of glucose homeostasis. They are essential for glucose‐stimulated insulin secretion from pancreatic β‐cells, contribute to the mechanisms by which hypoglycaemia stimulates glucagon release from pancreatic α‐cells, and are involved in glucose uptake into skeletal muscle, glucose production and release from the liver, and feeding behaviour. Not surprisingly, loss‐ or gain‐of‐function mutations in KATP channel genes have profound effects, giving rise to congenital hyperinsulinaemia and neonatal diabetes respectively. This symposium review focuses on our current understanding of the role of the KATP channel in glucose homeostasis in health and disease.
Angiostatin overexpression in Morris hepatoma results in decreased tumor growth but increased perfusion and vascularization.

UNLABELLED Growth of malignant tumors is dependent on sufficient blood supply. Thus, inhibition of tumor angiogenesis is emerging as a promising target in the treatment of malignancies. Human angiostatin (hANG) is one of the most potent inhibitors of endothelial cell proliferation, angiogenesis, and tumor growth in vivo. However, its mechanisms operating in vivo are not well understood. METHODS To obtain more information about functional changes in the angiogenic process, we established Morris hepatoma (MH3924A) cell lines expressing hANG (hANG-MH3924A). The effects of hANG expression on proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) were measured in coculture experiments in vitro. To evaluate changes in tumor perfusion and blood volume, H2 15O and 68Ga-DOTA-albumin (DOTA is 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid) were used for PET studies in vivo. Additionally, immunohistologic quantification of vascularization, apoptosis, and proliferation as well as gene array analyses were performed. RESULTS Our in vitro experiments demonstrate reduced proliferation and increased apoptosis in HUVECs when being cocultured with hANG-MH3924A. In support, tumor growth of hANG-MH3924A is diminished by 95% in vivo. However, tumor perfusion and blood volume are increased in hANG-MH3924A corresponding to an increased microvessel density. Furthermore, hANG-transfected tumors show changes in expression of genes related to apoptosis, stress, signal transduction, and metabolism. CONCLUSION hANG expression leads to inhibition of tumor growth, increased apoptosis, and changes in the expression of multiple genes involved in stress reactions, signal transduction, and apoptosis, which indicates a multifactorial reaction of tumors. An enhanced microvessel density is seen as part of these reactions and is associated with increased perfusion as measured by PET.
Modelling organisational innovation in UAE: investigating the love triangle involving leadership, organisational culture and innovation

This study uses a mixed method approach, comprising in-depth expert interviews and an intensive literature review to develop a conceptual study model of organisational innovation in the UAE. While leadership and culture are enacted differently in different contexts, very few of the rich existing related studies have been undertaken in the Middle East. Using data from an online survey of 370 employees, confirmatory factor analysis and structural equation modelling are used to test the study hypotheses. The findings demonstrate that organisational culture fully mediates the effect of leadership on innovation. This finding stands in sharp contrast with the current literature originating from other geographical contexts that demonstrated a positive direct effect of leadership on innovation. The reasons for this situation are discussed.
Persistent right coronary flow reserve at low perfusion pressure.

To determine whether right coronary (RC) flow reserve persists at perfusion pressures below the apparent autoregulatory range, the RC artery of 18 anesthetized dogs was cannulated and perfused at controlled pressures. RC blood flow (RCBF) fell from 65.3 +/- 6.1 to 33.7 +/- 2.3 ml.min-1.100 g-1 as RC perfusion pressure (RCPP) was reduced from 80 to 40 mmHg. At 40 mmHg, intracoronary adenosine increased RCBF by 97.9 +/- 10.6 ml.min-1.100 g-1 (P less than 0.001). RCBF fell to 9.5 +/- 1.7 ml.min-1.100 g-1 at 20 mmHg, and RCBF did not significantly increase during adenosine, although RC vasodilation was observed in four dogs. Regional right ventricular (RV) blood flows at RCPP of 80 and 40 mmHg were measured by radioactive microsphere technique. Before adenosine infusion, RCBF was distributed uniformly across the RV free wall at normal and low perfusion pressures. During adenosine infusion, blood flow in both regions increased significantly, but the flow reserve was greater in the subendocardial region at both normal and reduced pressures. RV myocardial O2 consumption (MVo2) was decreased significantly at 40 mmHg, however, there was no evidence of ischemia at this pressure, since the RV lactate extraction ratio was normal (n = 8). Thus RV O2 demand fell when RC O2 supply was reduced, although a flow reserve was available. RV MVo2 was restored to normal when right coronary flow reserve was mobilized by adenosine infusion. For RCBF from 65 to 365 ml.min-1.100 g-1, RC venous O2 content rose and RV MVo2 was essentially constant. (ABSTRACT TRUNCATED AT 250 WORDS)
The yeast ubiquitin‐like domain protein Mdy2 is required for microtubule‐directed nuclear migration and localizes to cytoplasmic granules in response to heat stress

MDY2 encodes a ubiquitin‐like (UBL)‐domain protein necessary for efficient mating in Saccharomyces cerevisiae. Unlike most UBL proteins, Mdy2 is apparently not subject to C‐terminal processing and is localized predominantly in the nucleus. Deletion of MDY2 is associated with a five‐ to seven‐fold reduction in mating efficiency, mainly due to defects in nuclear migration and karyogamy at the prezygotic stage. Here, we looked for two potential interacting partners of Mdy2, investigated the function of Mdy2 in nuclear movement, determined the increased heat sensitivity defects of mdy2Δ mutants, and inspected localization of Mdy2. Coprecipitation studies show that Mdy2 associates with α‐tubulin and with the microtubule (MT)‐associated dynactin subunit p150Glued/Nip100. nip100Δ mutants exhibit no defects in nuclear migration or in MT length or orientation during shmooing growth. Deletion of MDY2 display small nuclear migration phenotype during vegetative growth and seems to exacerbate the defects in mitotic nuclear migration seen in the nip100Δ strain. Deletion of MDY2 increased heat sensitivity of the cells and these strains accumulate mitotic nuclear migration defects and shortened MTs under these conditions. GFP‐Mdy2 proteins which are localized predominantly in the nucleus at permissive temperature are localized to cytoplasmic foci during heat shock. Colocalization studies revealed that heat stress‐induced enrichment of Mdy2 in cytoplasmic foci merged mainly with stress granules marker Pab1. During glucose deprivation a minority of Mdy2 foci overlapped with P‐bodies marker Dcp2, while most Mdy2 foci and Pab1 foci overlap. Accordingly, we propose that Mdy2 plays a critical role in the MT‐dependent processes of karyogamy and stress response. © 2010 Wiley‐Liss, Inc.
Anaerobic degradation of selected chlorinated hydrocarbon pesticides.

The persistence of many of the mod ern organic pesticides in the environ ment has led to a great deal of concern over their possible harmful effects on both wildlife and human beings. The most persistent of the organic pesti cides are the chlorinated hydrocarbon insecticides and certain associated epoxides, commonly referred to as a group simply as the chlorinated hydro carbons. Residues of these pesticides have been measured in soils more than a decade after application and traces have been found in almost all major U. S. rivers as well as in fish in the open sea. Chlorinated hydrocarbon residues in natural waters are of particular con cern because of their great toxicity to many aquatic organisms, because of their possible adverse effect on man through his drinking water supplies, and because of their tendency to con centrate in living matter which some times results in the death of higher species such as fish and birds, The extremely hydrophobic nature of many of the chlorinated hydrocarbons allows them to concentrate on particulate matter which may settle to form the bottom muds of streams and lakes.
Epidemiological, Clinical, Laboratory Findings, and Outcomes of Disseminated Tuberculosis in Tehran, Iran

Background: Disseminated tuberculosis (TB) accounts for 1 to 3% of all TB cases. This retrospective study reviews the clinical, radiological, laboratory findings and outcome in patients with disseminated tuberculosis in an endemic area. Methods: Medical records were reviewed for patients with disseminated TB admitted to two tertiary centers in Tehran, Iran between 1999 and 2006. Results: Fifty patients were found to have disseminated TB. A miliary pattern was documented in the chest x-ray of 34 patients. Hematologic abnormalities including anemia, leukopenia, and thrombocytopenia were frequently observed. Death occurred in nine of the cases. The mortality rate was significantly higher in diabetic patients, injection drug users, and patients with hematologic abnormalities; however, steroid usage and human immunodeficiency virus infection were not significantly associated with a higher mortality rate. Clinical improvement occurred in 41 patients following treatment. Conclusion: Disseminated TB could have different manifestations. Hematologic abnormalities are common and are considered poor prognostic signs in these patients.
Heparin‐Induced Thrombocytopenia

Each year, 25% of all hospitalized patients are affected by venous thromboembolism, a disease comprising deep vein thrombosis and pulmonary embolism, that is responsible for up to 10% of all inpatient deaths (Alikhan, Peters, Wilmott, Cohen, 2004). Used in both the treatment and the prevention of venous thromboembolism, heparin is administered to more than 12 million patients each year in the United States, making it one of the most widely prescribed medications and the leading intravenous anticoagulant. Heparin-induced thrombocytopenia is an antibody-mediated adverse drug reaction to heparin therapy with potentially life-threatening complications. This article discusses the pathogenesis, diagnosis, and treatment of patients with heparin-induced thrombocytopenia.
Revisiting the transitional dynamics of business cycle phases with mixed-frequency data

ABSTRACT This paper introduces a Markov-switching model in which transition probabilities depend on higher frequency indicators and their lags through polynomial weighting schemes. The MSV-MIDAS model is estimated through maximum likelihood (ML) methods with a slightly modified version of Hamilton’s filter. Monte Carlo simulations show that ML provides accurate estimates, but they suggest some caution in interpreting the tests of the parameters in the transition probabilities. We apply this new model to forecast business cycle turning points in the United States. We properly detect recessions by exploiting the link between GDP growth and higher frequency variables from financial and energy markets.
Identification of Staphylococcus xylosus Isolated from C57BL/6J‐Nos2tm1Lau Mice with Dermatitis

Coagulase negative staphylococci (CNS) are significant pathogens, particularly in medical device related infections and in immunocompromised patients. Five CNS strains were isolated from 5 NOS2 knockout mice with dermatitis. Histologically, granulomatous dermatitis was found in the skin around the ear with epidermal ulceration. Dermal lesions included pustules, necrosis, and accumulations of neutrophils and macrophages. Isolates of the bacterial strains were identified to be Staphylococcus xylosus by the API STAPH kit and 16S–23S intergenic transcribed spacer‐PCR. These results demonstrate the potential of this organism to be an opportunistic pathogen in immunocompromised mice.
The basic helix–loop–helix–PAS protein ARNT functions as a potent coactivator of estrogen receptor-dependent transcription

The biological effects of estrogens are mediated by the estrogen receptors ERα and ERβ. These receptors regulate gene expression through binding to DNA enhancer elements and subsequently recruiting factors such as coactivators that modulate their transcriptional activity. Here we show that ARNT (aryl hydrocarbon receptor nuclear translocator), the obligatory heterodimerization partner for the aryl hydrocarbon receptor and hypoxia inducible factor 1α, functions as a potent coactivator of ERα- and ERβ- dependent transcription. The coactivating effect of ARNT depends on physical interaction with the ERs and involves the C-terminal domain of ARNT and not the structurally conserved basic helix–loop–helix and PAS (Per-ARNT-Sim) motifs. Moreover, we show that ARNT/ER interaction requires the E2-activated ligand binding domain of ERα or ERβ. These observations, together with the previous role of ARNT as an obligatory partner protein for conditionally regulated basic helix–loop–helix–PAS proteins like the aryl hydrocarbon receptor or hypoxia inducible factor 1α, expand the cellular functions of ARNT to include regulation of ERα and ERβ transcriptional activity. ARNT was furthermore recruited to a natural ER target gene promoter in a estrogen-dependent manner, supporting a physiological role for ARNT as an ER coactivator.
Detection and sequencing of rotavirus VP7 gene from human materials (stools, sera, cerebrospinal fluids, and throat swabs) by reverse transcription and PCR

Human rotavirus RNAs from stool samples, sera, cerebrospinal fluids, and throat swabs of 15 children with rotavirus gastroenteritis were detected and serotyped by reverse transcription and PCR. The reverse transcription-PCR method may allow us to consider rotavirus infections in other parts of the body in addition to the gastrointestinal tract. Moreover, sequence analysis of the VP7 gene was performed on seven samples (one stool, two serum, three cerebrospinal fluid, and 1 throat swab sample). There were no appreciable differences in viral sequences between samples from cerebrospinal fluids, sera, or stools.
Do patients with maternal uniparental disomy for chromosome 7 have a distinct mild Silver-Russell phenotype?

Editor—Silver-Russell syndrome (SRS) is characterised by severe intrauterine growth retardation (IUGR), postnatal short stature, asymmetry of the face, body, and limbs, short and incurved fifth fingers, and a characteristic triangular face with a prominent and bossed forehead, a small lower jaw, and downturned corners of the mouth.1 2 Many other slightly dysmorphic features are seen in SRS patients (table 1) and these have been regarded mostly as confirmatory, not obligatory, for the diagnosis.3-5 Any one feature is not consistently observed in all SRS patients and the expression of features tends to vary greatly among subjects leading to considerable heterogeneity.3 4 Straightforward criteria for the evaluation of the severity of SRS characteristics have not been set and it has been proposed that SRS might in fact comprise different subgroups.4 6 Most SRS cases are sporadic, but different monogenic modes of inheritance have been proposed.7-9Abnormalities of chromosomes 8, 15, 17, and 18 have also been associated with SRS.10-15 View this table: Table 1 Occurrence of SRS features. Categorisation is based on multiple published reports of SRS features, on the general emphasis stated in these reports for inclusion criteria in SRS studies, and on our own observations Maternal uniparental disomy of chromosome 7 (matUPD(7)), the inheritance of both copies of chromosome 7 exclusively from the mother, occurs in approximately 10% of Silver-Russell syndrome (SRS) patients.16-19 Altogether, 21 cases of matUPD(7) have been reported6 16-24 and at least 14 of these have been diagnosed with SRS.6 16-19 All matUPD(7) patients have pre- and postnatal growth retardation, except one case who had short stature only postnatally.23 Paternal uniparental disomy of chromosome 7 has no effect on growth and development.25 It has been suggested that there is at least one imprinted gene influencing growth and development on …
Diffraction tomography and multisource holography applied to seismic imaging

Seismic tomography is emerging as an imaging method for determining subsurface structure. When the view‐angle coverage is limited and the scale of the medium inhomogeneities is comparable with the wavelength, as is often true in geophysical applications, the performance of ordinary ray tomography becomes poor. Other tomographic methods are needed to improve the imaging process. Here we study diffraction tomography and multisource holography and evaluate their performances for surface reflection profiling (SRP), vertical seismic profiling (VSP), and cross‐hole measurements. Theoretical formulations are derived for two‐dimensional geometry in terms of line sources along a source line and line receivers along a receiver line. The theory for diffraction tomography is based on the Born or Rytov approximation. The performances of diffraction tomography and multisource holography are evaluated by examining the information coverage in the spatial frequency domain and by numerical examples. Multisource holography,.