Miracle-dz/UniFAR-FTUs huggingface dataset:
An early life hypoxia event has a long-term impact on protein digestion and growth in juvenile European sea bass

ABSTRACT Ocean warming, eutrophication and the consequent decrease in oxygen lead to smaller average fish size. Although such responses are well known in an evolutionary context, involving multiple generations, this appears to be incompatible with current rapid environmental change. Instead, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes. Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared with the control group. We examined whether energy was used to cover higher costs for maintenance, digestion or activity metabolisms, as a result of differing metabolic rate. The lower growth was not a consequence of lower food intake. We measured several respirometry parameters and we only found a higher specific dynamic action (SDA) duration and lower SDA amplitude in a fish phenotype with lower growth; this phenotype was also associated with a lower protein digestive capacity in the intestine. Our results contribute to the understanding of the observed decrease in growth in response to climate change. They demonstrate that the reduced growth of juvenile fishes as a consequence of an early life hypoxia event was not due to a change of fish aerobic scope but to a specific change in the efficiency of protein digestive functions. The question remains of whether this effect is epigenetic and could be reversible in the offspring. Summary: The growth reduction in juvenile sea bass as a consequence of an early life hypoxia event is due to a specific change in the efficiency of protein digestive functions.
Utilization pattern of health care services at village level.

BACKGROUND Primary health care service is basic essential health care. Due to lack of access to quality health care services, many rural people suffer from various health related problems. The objective of this study was to assess how health care services are utilized by beneficiaries in two different villages in Dhanusha district of Nepal. METHODS A descriptive cross sectional study was conducted in two Village Development Committees with sixty four households selected randomly from January 1, to February 27, 2008. RESULTS The study shows that 48 and 21 percent of population were utilizing local available health services respectively within and beyond 2 km from the health facility . It revealed that distance was significant indicator for utilization of health services. Study also shows that those in VDC 1 had availed maximum (67%) service of trained man power for delivery as compared to those in VDC 2, (20%) where most (80%) of deliveries were conducted by relatives and neighbours, the difference was significant. CONCLUSIONS Distance from the heath services is an important factor for utilisation of health resources. The utilisation of health facility was satisfactory but the quality of services were not satisfactory.
Expansion of myeloid‐derived suppressor cells with aging in the bone marrow of mice through a NF‐κB‐dependent mechanism

With aging, there is progressive loss of tissue homeostasis and functional reserve, leading to an impaired response to stress and an increased risk of morbidity and mortality. A key mediator of the cellular response to damage and stress is the transcription factor NF‐κB. We demonstrated previously that NF‐κB transcriptional activity is upregulated in tissues from both natural aged mice and in a mouse model of a human progeroid syndrome caused by defective repair of DNA damage (ERCC1‐deficient mice). We also demonstrated that genetic reduction in the level of the NF‐κB subunit p65(RelA) in the Ercc1−/∆ progeroid mouse model of accelerated aging delayed the onset of age‐related pathology including muscle wasting, osteoporosis, and intervertebral disk degeneration. Here, we report that the largest fraction of NF‐κB ‐expressing cells in the bone marrow (BM) of aged (>2 year old) mice (C57BL/6‐NF‐κBEGFP reporter mice) are Gr‐1+CD11b+myeloid‐derived suppressor cells (MDSCs). There was a significant increase in the overall percentage of MDSC present in the BM of aged animals compared with young, a trend also observed in the spleen. However, the function of these cells appears not to be compromised in aged mice. A similar increase of MDSC was observed in BM of progeroid Ercc1−/∆ and BubR1H/H mice. The increase in MDSC in Ercc1−/∆ mice was abrogated by heterozygosity in the p65/RelA subunit of NF‐κB. These results suggest that NF‐κB activation with aging, at least in part, drives an increase in the percentage of MDSCs, a cell type able to suppress immune cell responses.
How do flexible work arrangements alleviate work-family-conflict? The roles of flexibility i-deals and family-supportive cultures

Abstract Flexible work arrangements (FWAs) are becoming common in emerging economies, though it is not clear if and how employees benefit from them in terms of their work-family balance and well-being. In contrast to previous work that used structural-functional explanations, we argue that FWA implementation should be depicted within its socio-political and socio-cultural context. We used survey data collected from 213 professionals in Istanbul, Turkey to examine the roles of flexibility i-deals and perceived family-supportive culture in the relationship between FWA availability and these employee outcomes. Results supported the hypothesis that i-deals mediate the relationship between FWAs and work-to-family conflict. Furthermore, perceived family supportive cultures predicted both work-to-family and family-to-work conflict and moderated the relationship between i-deals and family-to-work conflict. Finally work-to-family conflict mediated the relationship of flexibility i-deals and family supportive cultures with perceived general health. These finding are discussed for their implications for employees who are trying to balance their work and family lives and for organizations who are considering implementing FWAs.
The dominance of behavioural activation over behavioural inhibition in conduct disordered boys with or without attention deficit hyperactivity disorder.

On the basis of Gray's theory, Quay suggested that conduct disorder (CD) is associated with a Behavioural Activation System (BAS) that dominates over the Behavioural Inhibition System (BIS), whereas attention deficit hyperactivity disorder (ADHD) is characterised by an underactive BIS. Two studies were conducted to test the hypothesis that the dominance of the BAS over the BIS is more pronounced in CD comorbid with ADHD (CD/ADHD) than in CD alone. First of all, a response perseveration task was used, i.e. the door-opening task (Daugherty & Quay, 1991). In this game, the subject chooses either to open the next door or to stop playing; there is a steadily increasing ratio of punished responses to rewarded responses and a large number of doors opened is indicative of response perseveration. As expected, a steady increase in the number of doors opened was found across normal control (NC) boys, CD boys, and CD/ADHD boys (NC < CD < CD/ADHD). Second, the dominance of the BAS over the BIS was examined by observing the social behaviour of the child in interaction with a research assistant who alternately activated the BAS and the BIS while a game was played. The behaviour of the children was analysed according to ethological methods. Group differences in the frequencies of three out of five behavioural categories were in line with the results of the door-opening task (NC < CD < CD/ADHD).
Insights into the human brain proteome: Disclosing the biological meaning of protein networks in cerebrospinal fluid

Abstract Cerebrospinal fluid (CSF) is an excellent source of biological information regarding the nervous system, once it is in close contact and accurately reflects alterations in this system. Several studies have analyzed differential protein profiles of CSF samples between healthy and diseased human subjects. However, the pathophysiological mechanisms and how CSF proteins relate to diseases are still poorly known. By applying bioinformatics tools, we attempted to provide new insights on the biological and functional meaning of proteomics data envisioning the identification of putative disease biomarkers. Bioinformatics analysis of data retrieved from 99 mass spectrometry (MS)-based studies on CSF profiling highlighted 1985 differentially expressed proteins across 49 diseases. A large percentage of the modulated proteins originate from exosome vesicles, and the majority are involved in either neuronal cell growth, development, maturation, migration, or neurotransmitter-mediated cellular communication. Nevertheless, some diseases present a unique CSF proteome profile, which were critically analyzed in the present study. For instance, 48 proteins were found exclusively upregulated in the CSF of patients with Alzheimer’s disease and are mainly involved in steroid esterification and protein activation cascade processes. A higher number of exclusively upregulated proteins were found in the CSF of patients with multiple sclerosis (76 proteins) and with bacterial meningitis (70 proteins). Whereas in multiple sclerosis, these proteins are mostly involved in the regulation of RNA metabolism and apoptosis, in bacterial meningitis the exclusively upregulated proteins participate in inflammation and antibacterial humoral response, reflecting disease pathogenesis. The exploration of the contribution of exclusively upregulated proteins to disease pathogenesis will certainly help to envision potential biomarkers in the CSF for the clinical management of nervous system diseases.
Toxoplasma gondii: humoral and cellular immune response of BALB/c mice immunized via intranasal route with rTgROP2.

TgROP2 is an intracellular protein associated with rhoptries of Toxoplama gondii and an antigen component of a candidate vaccine for toxoplasmosis. The purpose of the present study was to evaluate the efficacy of rTgROP2 to stimulate humoral and cellular immune responses in BALB/c mice via intranasal injection. TgROP2 partial coding sequence was (196-561) amplified by PCR from genomic T. gondii RH strain DNA and cloned into the pTrcHis expression vector. Escherichia coli Rosetta 2 cells transformed with pTrcHis-TgROP2 showed high levels (~1 mg.mL(-1)) of recombinant protein after 4 hours of IPTG induction. Recombinant TgROP2 exhibited an apparent Mr equal to 54 kDa. In order to test immunogenicity of the recombinant protein, 10 BALB/c mice received 10 µg of rROP2 protein + 10 µg of Quil-A via intranasal injection. Doses were administered at days 0, 21, and 42. Three animals were euthanized and used to evaluate cellular immune response on day 62. Five (50%) and two (20%) out of ten animals produced IgG (DO mean = 0.307; cut-off = 0.240) and IgA (DO mean = 0.133, cut-off = 0.101), respectively, by ELISA on day 62. The proliferation of splenocytes revealed high stimulation index (SI) when co-cultured with 5, 10 and 15 µg. mL(-1) of rTgROP2. These results indicate that intranasal immunization with recombinant protein ROP2 plus Quil-A can elicit both cellular and humoral immune responses in BALB/c mice.
Effect of nonsurgical periodontal therapy during gestation period on adverse pregnancy outcome: a systematic review

Abstract Objective: To confirm effectiveness of nonsurgical periodontal treatment during pregnancy on pregnancy outcome in terms of preterm birth and low birth weight (LBW). Method: We searched for Randomized controlled trials in MEDLINE, CINAHL and EMBASE. from January 2000 to October 2012. References of included studies were hand searched. Studies which measured incidence of either preterm labor (PTL), LBW or both were included. Information on study design, population, risk of bias, periodontal parameters, time and type of periodontal treatment given and pregnancy outcome were abstracted independently by three reviewers. Results: Total thirteen studies were included. Five studies had low risk of bias. Conclusive results could not be obtained for incidence of PTL or LBW because of heterogeneity in results due to various reasons, but all studies which assessed PTL and LBW combined showed significant difference in incidence of same in group which received non surgical periodontal treatment. Conclusion: Our study suggests that treatment of periodontitis during gestation improves pregnancy outcome in terms of PTLBW, a fact which may be used for health and economical benefits but better RCTs with adequate sample size are required for conclusive evidence.
From Q Fever to Coxiella burnetii Infection: a Paradigm Change

SUMMARY Coxiella burnetii is the agent of Q fever, or “query fever,” a zoonosis first described in Australia in 1937. Since this first description, knowledge about this pathogen and its associated infections has increased dramatically. We review here all the progress made over the last 20 years on this topic. C. burnetii is classically a strict intracellular, Gram-negative bacterium. However, a major step in the characterization of this pathogen was achieved by the establishment of its axenic culture. C. burnetii infects a wide range of animals, from arthropods to humans. The genetic determinants of virulence are now better known, thanks to the achievement of determining the genome sequences of several strains of this species and comparative genomic analyses. Q fever can be found worldwide, but the epidemiological features of this disease vary according to the geographic area considered, including situations where it is endemic or hyperendemic, and the occurrence of large epidemic outbreaks. In recent years, a major breakthrough in the understanding of the natural history of human infection with C. burnetii was the breaking of the old dichotomy between “acute” and “chronic” Q fever. The clinical presentation of C. burnetii infection depends on both the virulence of the infecting C. burnetii strain and specific risks factors in the infected patient. Moreover, no persistent infection can exist without a focus of infection. This paradigm change should allow better diagnosis and management of primary infection and long-term complications in patients with C. burnetii infection.
Adherent-invasive Escherichia coli and Crohn's disease

Purpose of review The identification of mutations in the nucleotide oligomerization domain 2-encoding gene in patients with Crohn's disease suggests a link between the innate immune response to invasive bacteria and the development of Crohn's disease. Herein, we review reports concerning the association of pathogenic Escherichia coli with the intestinal mucosa of Crohn's disease patients. Recent findings Adherent-invasive E. coli were isolated from ileal biopsies of 36.4% of patients with ileal Crohn's disease. Adherent-invasive E. coli colonize the intestinal mucosa by adhering to intestinal epithelial cells. They are also true invasive pathogens, able to invade intestinal epithelial cells and to replicate intracellularly. Adherent-invasive E. coli strains replicate extensively into macrophages inducing the secretion of very large amounts of tumor necrosis factor-α. Similar pathogenic E. coli strains were recently associated with granulomatous colitis of Boxer dogs. Interestingly, high levels of E. coli outer membrane protein C antibodies are present in 37–55% of Crohn's disease patients and reactivity to outer membrane protein C is associated with increased severity of Crohn's disease. Summary As the infection cycle of adherent-invasive E. coli could depend upon the ability of these pathogenic bacteria to colonize the gastrointestinal tract of genetically predisposed Crohn's disease patients, antibiotics which could eradicate the bacteria, or probiotics which could substitute them in the gastrointestinal tract, could be of therapeutic value in ileal Crohn's disease.
Sound velocities and hypersonic dampings of Pb[(Mg1/3Nb2/3)0.45Ti0.55]O3 single crystals studied by Brillouin light scattering

A Brillouin spectroscopic investigation was carried out on PMN–55%PT single crystals, which are known to have no chemically ordered regions and undergo a well-defined structural phase transition at TC ∼ 521 K. The longitudinal and transverse sound velocities probed on a right-angle scattering geometry exhibited a remarkable softening and increasing hypersonic damping on approaching TC from TB ∼ 610 K that was characterized by the deviation of the dielectric permittivity from the high-temperature Curie–Weiss behavior. The acoustic anomalies of the longitudinal acoustic mode at the backward scattering were more substantial than those observed at the right-angle scattering, which could be understood in the framework of normal acoustic dispersion considering the difference in the acoustic frequency. The softening of the transverse sound velocity was more significant than that of the longitudinal one upon cooling toward TC, suggesting that this acoustic anomaly may be related to the local rhombohedral transformation, occurring in polar nano-regions (PNRs). The observed acoustic behaviors combined with the central peak dynamics clearly indicated the existence of dynamic polar nano-regions in PMN–55%PT where there are no chemically ordered regions, and seem to suggest that the order parameter fluctuations due to two kinds of coupling contribute to the acoustic anomalies in the temperature range of TC ∼ TB: electrostrictive coupling between the acoustic waves and the dynamic PNRs, and linear coupling between the acoustic waves and the precursor polar clusters, i.e., the ordering unit responsible for the order–disorder-type slowing down behavior probed by the central peak.
Growth and mineral acquisition response of grapevine rootstocks (Vitis spp.) to inoculation with different strains of plant growth-promoting rhizobacteria (PGPR).

BACKGROUND Effects of the plant growth-promoting rhizobacteria (PGPR) strains Burkholderia gladii BA-7, Bacillus subtilis OSU-142, Bacillus megatorium M-3 and Azospirillum brasilense Sp 245 on vegetative development and mineral uptake of 1103 P and 41 B grapevine rootstocks were investigated. The roots of nursery plants of the grapevine rootstocks were immersed in bacterial solutions and transplanted to a sterilised peat and perlite mixture in 5 L pots. Plants were cultivated in a semi-controlled glasshouse during the vegetation period. RESULTS Vegetative development of grapevine rootstocks was obviously promoted by bacterial inoculation, with the maximum increase induced by Sp 245. Inoculation with Sp 245 also significantly improved the chlorophyll concentrations of the leaves of the two rootstocks. Among the bacteria, OSU-142 also significantly stimulated vegetative development and mineral acquisition of the plants. Nutrient contents of the leaf blades of the plants were generally higher than those of control plants. CONCLUSION Overall investigations revealed that A. brasilense Sp 245 and B. subtilis OSU-142 performed more efficiently than the other strains. Therefore these bacteria seem to have considerable potential in reducing the need for inorganic fertiliser.
Prediction of Persistence or Recurrence After Conization for Cervical Intraepithelial Neoplasia III

OBJECTIVE: To estimate the predictive factors for persistent/recurrent disease before and after conization for cervical intraepithelial neoplasia III. METHODS: Patients who received conization due to histologic diagnosis of cervical intraepithelial neoplasia III from 1998 to 2000 and who had at least one cytologic/histologic follow-up within one year of conization (n = 449) were enrolled in our study. All available demographic and pathologic parameters were analyzed. RESULTS: We performed multivariable logistic regression analysis to identify predictive factors for cervical intraepithelial neoplasia III persistence/recurrence. Age (greater than 50 years) was the only preoperative predictor and had an odds ratio equaling 3.070 (95% confidence interval [CI] 1.421–6.630, P = .004). Post–cone endocervical curettage was found to be the most statistically significant factor for predicting persistent disease (odds ratio 7.940, 95% CI 3.428–18.390, P < .001). Positive endocervical curettage was associated with 65.5% (36/55) of persistent disease, whereas negative endocervical curettage was associated with only 7.6% (26/342). Positive endocervical resection margins and multiple-quadrant disease also had predictive values with odds ratios equaling 2.972 (95% CI 1.401–6.281, P = .004) and 2.180 (95% CI 1.014–4.689, P = .046), respectively. The positive predictive values for age (> 50 years), positive endocervical curettage, positive endocervical resection margin, and multiple quadrant disease were 31.7%, 65.5%, 40.0%, and 21.9%, respectively. CONCLUSION: We found that age is the only preoperative predictive factor. Pathologic parameters, including endocervical curettage, endocervical resection margins, and multiple-quadrant disease are the only postoperative predictive factors for cervical intraepithelial neoplasia persistence or recurrence found in our study. These factors should be considered in patient management before and after therapeutic conization for cervical intraepithelial neoplasia III. LEVEL OF EVIDENCE: II-3
A swimming-associated outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7 and Shigella sonnei.

BACKGROUND In the summer of 1991, simultaneous outbreaks of bloody diarrhea and hemolytic-uremic syndrome caused by Escherichia coli O157:H7 and of bloody diarrhea caused by Shigella sonnei were traced to a lakeside park near Portland, Oregon. METHODS We identified cases primarily from routine surveillance reports. In case-control studies, the activities of persons with park-associated E. coli O157:H7 or S. sonnei infections were compared independently with those of three sets of controls. We also evaluated environmental conditions at the park and subtyped the bacterial isolates. RESULTS We identified 21 persons with park-associated E. coli O157:H7 infections (all of them children; median age, six years) and 38 persons with S. sonnei infections (most of them children). These 59 people had visited the park over a 24-day period. Their illnesses were not associated with food or beverage consumption. All the case patients reported swimming, however, and in case-control studies swimming was strongly associated with both types of infection (P = 0.015 or less). The case patients were more likely than the controls to report having swallowed lake water, and they had spent more time in the lake. Numbers of enterococci indicative of substantial fecal contamination (geometric mean, > 50 per deciliter) were detected in the swimming area during some but not all of the outbreak period. Park-associated E. coli O157:H7 isolates were identical by pulsed-field gel electrophoresis and were distinguishable from other isolates in the Portland area. CONCLUSIONS Lake water that was fecally contaminated by bathers was the most likely vehicle for the transmission of both the E. coli O157:H7 and the S. sonnei infections. The unusually prolonged outbreak suggests both the survival of these enteric organisms in lake water and a low infectious dose.