Miracle-dz/UniFAR-FTUs huggingface dataset:
CaMKII “Autonomy” Is Required for Initiating But Not for Maintaining Neuronal Long-Term Information Storage

Ca2+/calmodulin (CaM)-dependent protein kinase II (CaMKII) “autonomy” (T286-autophosphorylation-induced Ca2+-independent activity) is required for long-term potentiation (LTP) and for learning and memory, as demonstrated by CaMKII T286A mutant mice. The >20-year-old hypothesis that CaMKII stimulation is required for LTP induction, while CaMKII autonomy is required for LTP maintenance was recently supported using the cell-penetrating fusion-peptide inhibitor antCN27. However, we demonstrate here that ant/penetratin fusion to CN27 compromised CaMKII-selectivity, by enhancing a previously unnoticed direct binding of CaM to ant/penetratin. In contrast to antCN27, the improved cell-penetrating inhibitor tatCN21 (5 μm) showed neither CaM binding nor inhibition of basal synaptic transmission. In vitro, tatCN21 inhibited stimulated and autonomous CaMKII activity with equal potency. In rat hippocampal slices, tatCN21 inhibited LTP induction, but not LTP maintenance. Correspondingly, tatCN21 also inhibited learning, but not memory storage or retrieval in a mouse in vivo model. Thus, CaMKII autonomy provides a short-term molecular memory that is important in the signal computation leading to memory formation, but is not required as long-term memory store.
The Effect of Corporate Diversification on the Profitability of the Financial Services Sector in Nigeria

This paper examines the effect of corporate diversification on the profitability of the Financial services sector in Nigeria. The study relied on historic accounting data generated from financial (annual) reports and accounts of sampled banks between the period 1998 and 2007 (a ten-year period). A regression equation was formulated, in line with previous studies to shed light on the effect of corporate diversification on the profitability of the Financial services sector in Nigeria. The results of the regression analysis revealed that diversification impacts strongly on banks profitability. Conclusively the paper produces strong evidence to assert that diversification impacts positively and significantly on banks profitability because among other things such diversified banks can pool their internally generated funds and allocate them properly. Keywords—Diversification; firm size; operational efficiency; profitability
Dynamic dissipative compensators for multibody flexible space structures

The stability characteristics of dynamic dissipative compensators are investigated for multibody flexible space structures having nonlinear dynamics. The problem addressed is that of proving asymptotic stability of dynamic dissipative compensators. The stability proof uses the Lyapunov approach and exploits the inherent passivity of such systems. For such systems these compensators are shown to be robust to parametric uncertainties and unmodeled dynamics. The results are applicable to a large class of structures such as satellites with multiple payloads and space-based manipulators. <<ETX>>
TAP‐translocated peptides specifically bind proteins in the endoplasmic reticulum, including gp96, protein disulfide isomerase and calreticulin

The endoplasmic reticulum (ER) membrane‐embedded transporter associated with antigen processing (TAP) associates with peptides in the cytosol and translocates these into the ER lumen. Here, MHC class I molecules bind a subset of these peptides and the remainder is either removed or degraded, or may be retained in the ER in association with other proteins. We have visualized peptide‐binding proteins in the ER using radioactive peptides with a photoreactive group. Besides TAP, two proteins were identified as gp96 and protein disulfide isomerase (PDI). Calreticulin, previously found in complex with TAP, only binds glycosylated peptides. In addition, two as yet unidentified, ER luminal glycoproteins (gp120 and gp170) were visualized. The effects of peptide size and sequence on binding to the ER‐resident proteins were studied by using partially degenerated peptides with photoreactive side chains. All identified proteins were able to bind peptides within the size range of peptides translocated by TAP, from 8 to more than 20 amino acids. Whereas PDI associated with all peptides tested, gp96 and gp120 showed a clear sequence preference for non‐charged amino acids at positions 2 and 9 in 9mer peptides. Thus various ER proteins, other than the MHC class I heterodimer and TAP, are able to interact with peptides albeit with a different substrate selectivity.
Evidence for lymphangiogenesis and its prognostic implications in head and neck squamous cell carcinoma

Lymph node metastasis is a frequent reason for adverse clinical outcome in many epithelial neoplasms, including head and neck squamous cell carcinoma. The mechanisms underlying the capability of epithelial neoplasms to metastasize via lymphatic vessels have not yet been fully elucidated. There is great debate about whether cancer cells can metastasize by expansion and invasion of pre‐existing peritumoral lymphatics or by the formation and invasion of new lymphatics within tumours (lymphangiogenesis). In order to investigate this issue, we examined 81 tissue specimens from patients with head and neck squamous cell carcinoma, using immunostaining for the specific lymphatic endothelium marker podoplanin, and assessed intratumoral and peritumoral lymphatic density. We also quantified lymphatic invasion and examined the possible associations of all the above parameters with clinicopathological features and outcome. Finally, we used double staining with podoplanin and the cell proliferation marker Ki‐67 in order to evaluate lymphangiogenesis. High intratumoral and peritumoral lymphatic density were both significantly associated with the presence of lymph node metastasis at the time of diagnosis (χ2 test, p < 0.001 and p = 0.007, respectively) and there was a significant correlation between high intratumoral lymphatic density and lymphatic invasion. Patients with higher intratumoral lymphatic density exhibited shorter overall survival (log rank p < 0.001) and this correlation remained significant after multivariate analysis (Cox p = 0.04), indicating that intratumoral lymphatic density is an independent prognostic factor for mortality. Peritumoral lymphatic density had no influence on outcome. Double staining revealed the existence of proliferating intratumoral lymphatics, in which tumour emboli were occasionally observed. These results indicate that lymphangiogenesis indeed occurs in head and neck squamous cell carcinoma; that newly formed vessels are targets of invasion by cancer cells; and that intratumoral lymphatic density might be used as a criterion to separate patients at higher risk of an adverse clinical outcome. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Environmental persistence of Bacillus thuringiensis spores following aerial application.

Soil and leaf populations of Bacillus thuringiensis (Bt) were monitored following aerial application of commercial Bt formulations at the rate of 72 billion international units per acre per year during a 5-year period. Data from soil sample spore counts suggested that Bt spores persisted in Wasatch forest soils for up to 2 years but they did not proliferate. Bt isolates were recovered from leaf samples 12 months post application from sprayed, previously sprayed and from nonsprayed areas. The frequency and diversity of Bt isolates recovered from leaves was independent of sample area spray history. In accordance with U.S. Forest Service criteria, aerial application of Bt during a 5-year period resulted in the eradication of gypsy moth (Lymantria dispar, L) from the Wasatch Front region of the Wasatch Mountain Range, Utah.
A comparative study of storage stability in virgin coconut oil and extra virgin olive oil upon thermal treatment

This aim of this research is to investigate thermal stability of virgin coconut oil, (VCO) which was heated at 190°C upon 40 days storage as compared to extra virgin olive oil (EVOO). The changes in fatty acids composition through (GC), Fourier Transform Infrared (FTIR) spectra, iodine value (IV) and total phenolic content were determined throughout the period of study. Results from GC showed that there was significant changes (P<0.05) in the percentage of linolenic acid (from 0.74% to 0.54%) and palmitic acid (from 12.31% to 13.44%) in the EVOO upon storage. Higher stability was found in VCO as it contains higher saturated fatty acids. FTIR spectra showed both oils had undergone oxidation and produced hydroperoxides upon 40 days storage but with less oxidation in VCO. Meanwhile, IV and total phenolic content decreased significantly (P<0.05) in both oils with percentage reduction of IV in VCO greater than that in EVOO (70.60% vs. 11.90%), contrary to percentage loss in total phenolic content (50.77% vs. 68.19%). As a conclusion, VCO can be considered as good frying oil as it has relatively high oxidative stability as EVOO.
Multicenter U.S. Bilateral MED-EL Cochlear Implantation Study: Speech Perception over the First Year of Use

Objective: Binaural hearing has been shown to support better speech perception in normal-hearing listeners than can be achieved with monaural stimulus presentation, particularly under noisy listening conditions. The purpose of this study was to evaluate whether bilateral electrical stimulation could confer similar benefits for cochlear implant listeners. Design: A total of 26 postlingually deafened adult patients with short duration of deafness were implanted at five centers and followed up for 1 yr. Subjects received MED-EL COMBI 40+ devices bilaterally; in all but one case, implantation was performed in a single-stage surgery. Speech perception testing included CNC words in quiet and CUNY sentences in noise. Target speech was presented at the midline (0 degrees), and masking noise, when present, was presented at one of three simulated source locations along the azimuth (−90, 0, and +90 degrees). Results: Benefits of bilateral electrical stimulation were observed under conditions in which the speech and masker were spatially coincident and conditions in which they were spatially separated. Both the “head shadow” and “summation” effects were evident from the outset. Benefits consistent with “binaural squelch” were not reliably observed until 1 yr after implantation. Conclusions: These results support a growing consensus that bilateral implantation provides functional benefits beyond those of unilateral implantation. Longitudinal data suggest that some aspects of binaural processing continue to develop up to 1 yr after implantation. The squelch effect, often reported as absent or rare in previous studies of bilateral cochlear implantation, was present for most subjects at the 1 yr measurement interval.
Distribution of ABO and Rhesus-D blood groups in and around Bangalore

Sir, The frequencies of ABO and Rhesus-D blood groups vary from one population to another. There are no data available for Bangalore, Karnataka. Our aim was to determine the distribution of different blood groups in this region. Blood group determination was carried out for 8 years, from January 2000 to December 2007, and encompassed 36,964 subjects donating blood to the transfusion medicine center of a neurological tertiary care institute. ABO and Rh blood grouping was done by using commercially available anti-sera A, B, AB, H and Rh (D), and known cells prepared, in-house, from pooled blood units, were used. For typing of Rh, we did not use other anti-sera like anti-c, anti-C, anti-e, anti-E; but only anti-D, which is most immunogenic. Hence those who tested positive with anti-sera D were considered to be Rh positive and those who did not were considered to be Rh negative. These anti-sera were validated at our laboratories before using them. For determination of ABO blood groups, both forward and reverse groupings were carried out. The results were analyzed and data compiled. Our study involving 36,964 donors, both male and female, showed O group to be high, viz., 14,716 (39.81%) donors, followed by B group having 11,071 (29.95%) donors and A at 8,817 (23.85%) and AB at 2,358 (6.37%) donors being the lowest. Rh-D blood group frequency was 94.20% positive and 5.79% negative, which shows that it follows the Asiatic trend of O > B > A > AB. There were only 2 (0.005%) donors out of 36,964 with Bombay blood group (Oh). Few studies of ABO and Rh blood group prevalence among the various populations of India have been carried out. Study done by Nanu and Thapliyal in the north Indian population report that group B is the most predominant one,[1] as also reported in a study in neighboring Pakistan. [1,2] The south Indian study by Das et al. shows that group O is the most predominant one, followed by group B and group A, which is in agreement with our study; and also, the finding regarding Rh negativity was almost similar to that from our study. [3] Another south Indian study conducted on the population of Chittoor district of Andhra Pradesh also showed similar pattern of distribution of blood groups. [4] It is hoped that the data generated in this study would assist in the planning and establishment of a functional blood service that would meet the ever-increasing demand for safe blood and blood products.
Ready for practice? A study of confidence levels of final year dental students at Cardiff University and University College Cork.

AIM The aim of this study was to describe the self-reported confidence levels of final year students at the School of Dentistry, Cardiff University and at the University Dental School & Hospital, Cork, Ireland in performing a variety of dental procedures commonly completed in primary dental care settings. METHOD A questionnaire was distributed to 61 final year students at Cardiff and 34 final year students at Cork. Information requested related to the respondents confidence in performing a variety of routine clinical tasks, using a five-point scale (1=very little confidence, 5=very confident). Comparisons were made between the two schools, gender of the respondent, and whether or not a student intended completing a year of vocational training after graduation. RESULTS A response rate of 74% was achieved (n=70). The greatest self-reported confidence scores were for 'scale and polish' (4.61), fissure sealants (4.54) and delivery of oral hygiene instruction (4.51). Areas with the least confidence were placement of stainless steel crowns (2.83), vital tooth bleaching (2.39) and surgical extractions (2.26). Students at Cardiff were more confident than those at Cork in performing simple extractions (Cardiff: 4.31; Cork: 3.76) and surgical extractions (Cardiff: 2.61; Cork: 1.88), whilst students in Cork were more confident in caries diagnosis (Cork: 4.24; Cardiff: 3.89) fissure sealing (Cork: 4.76; Cardiff: 4.33) and placement of preventive resin restorations (Cork: 4.68; Cardiff: 4.22). CONCLUSION Final year students at Cardiff and Cork were most confident in simpler procedures and procedures in which they had had most clinical experience. They were least confident in more complex procedures and procedures in which they had the least clinical experience. Increased clinical time in complex procedures may help in increasing final year students' confidence in those areas.
Nuclear Localization and Regulation of Id Protein through an E Protein-mediated Chaperone Mechanism*

Members of the Id family of helix-loop-helix proteins function as negative regulators of DNA binding, E protein, helix-loop-helix transcription factors in the control of cell growth, differentiation, and development. By using transient transfection analysis of COS cells, we show that in the absence of its E protein target, the Id3 protein is localized exclusively to the cytoplasm/perinuclear region. Co-transfection with E protein (E47) results in nuclear translocation of the Id3 protein, a process requiring both a functional Id helix-loop-helix dimerization domain and an E protein nuclear localization signal. Id3 that is associated with E protein displays an extended half-life, while the E protein itself is more rapidly turned over. These observations demonstrate that E protein, by nuclear chaperoning Id, can regulate the available cellular pool of its own inhibitory partner.
Decision making in analyses of women's birth stories.

BACKGROUND Complex interactions occur among women and caregivers throughout labor. Analyzing women's birth stories provides a rich data source on these interactions. The purpose of this qualitative study was to clarify how decisions were made in labor by analyzing women's birth stories. METHODS A convenience sample of 15 primiparous and multiparous Midwestern women contributed a total of 33 birth stories. Qualitative methods were used, including analyses of the content and themes of stories. RESULTS The primary types of decision making that were identified ranged on a continuum from unilateral to joint (shared), and were associated with various emotions expressed by the women. CONCLUSIONS A model of decision making was derived from the data that may help caregivers change practices in ways that will benefit women. Caregivers can also benefit by understanding women's critiques of the birth care they received, and can use this knowledge to improve women's experiences of birth and therefore their satisfaction with the process.
Evolution of seed dormancy due to sib competition: effect of dispersal and inbreeding.

The effect of dispersal and inbreeding on the evolution of seed dormancy to avoid sib competition is theoretically investigated, using a model which assumes a plant population with patchy spatial structure in a constant environment. Applying the inclusive fitness method, the evolutionarily stable dormancy rates are analytically derived for three cases: (a) an asexual haploid population, (b) a diploid-hermaphrodite population in which the dormancy rate is controlled by seeds, and (c) a diploid-hermaphrodite population in which the dormancy rate is controlled by mother plants. The evolutionarily stable dormancy rates decrease in the order of case (c), case (a), and case (b). In all the cases, the evolutionarily stable dormancy rates increase with decreasing the dispersal rate. Although inbreeding generally increases the evolutionarily stable dormancy rates, inbreeding due to selfing reduces the rate exceptionally in case (c).
Emerging Roles of Transforming Growth Factor β Signaling in Diabetic Retinopathy

Diabetic retinopathy (DR) is a serious complication of diabetes mellitus affecting about one third of diabetic adults. Despite its prevalence, treatment options are limited and often implemented only in the later stages of the disease. To date, the pathogenesis of DR has been extensively characterized in the context of elevated glucose, insulin, and VEGF signaling, although a growing number of other growth factors and molecules, including transforming growth factor β (TGF‐β) are being recognized as important contributors and/or therapeutic targets. Here, we review the complex roles of TGF‐β signaling in DR pathogenesis and progression. J. Cell. Physiol. 232: 486–489, 2017. © 2016 Wiley Periodicals, Inc.
Relative Importance of Emotional Dysregulation, Hostility, and Impulsiveness in Predicting Intimate Partner Violence Perpetrated by Men in Alcohol Treatment

The current study employs dominance analysis to assess the relative importance of three constructs—hostility, impulsiveness, and emotional dysregulation (difficulties managing one’s emotions when experiencing negative emotion or distress)—in explaining psychological, physical, and sexual intimate partner violence (IPV) perpetration by men seeking alcohol treatment. A sample of 121 predominantly White, heterosexual men (average age 33.28, range = 18–62) enrolled in residential substance abuse treatment completed measures of emotional dysregulation, hostility, and impulsiveness, which are three highly related constructs identified as risk factors for both substance use disorders and IPV. The constructs collectively accounted for 20–25% of the variance in each form of IPV. Because impulsiveness, hostility, and emotional dysregulation are highly correlated, dominance analysis was used to examine which factor most strongly predicted each form of IPV. Dominance analysis findings favored hostility as a predictor of physical IPV perpetration and impulsiveness as a predictor of sexual and psychological IPV perpetration. Differential associations between the constructs and each form of IPV may be used to inform assessment and treatment planning of men who abuse alcohol. Better understanding, preventing, and treating male-to-female IPV will protect women from the far-reaching consequences of this violence.
Functional Analysis of the Epidermal-Specific MYB Genes CAPRICE and WEREWOLF in Arabidopsis[W]

Epidermis cell differentiation in Arabidopsis thaliana is a model system for understanding the developmental end state of plant cells. Two types of MYB transcription factors, R2R3-MYB and R3-MYB, are involved in cell fate determination. To examine the molecular basis of this process, we analyzed the functional relationship of the R2R3-type MYB gene WEREWOLF (WER) and the R3-type MYB gene CAPRICE (CPC). Chimeric constructs made from the R3 MYB regions of WER and CPC used in reciprocal complementation experiments showed that the CPC R3 region cannot functionally substitute for the WER R3 region in the differentiation of hairless cells. However, WER R3 can substantially substitute for CPC R3. There are no differences in yeast interaction assays of WER or WER chimera proteins with GLABRA3 (GL3) or ENHANCER OF GLABRA3 (EGL3). CPC and CPC chimera proteins also have similar activity in preventing GL3 WER and EGL3 WER interactions. Furthermore, we showed by gel mobility shift assays that WER chimera proteins do not bind to the GL2 promoter region. However, a CPC chimera protein, which harbors the WER R3 motif, still binds to the GL2 promoter region.
A Transformerless Hybrid Active Filter Using a Three-Level Pulsewidth Modulation (PWM) Converter for a Medium-Voltage Motor Drive

This paper presents a transformerless hybrid active filter integrated into a medium-voltage motor drive for energy savings. This hybrid filter is intended for line harmonic-current mitigation of the three-phase diode rectifier used as the front end of the motor drive. It is based on direct connection of a passive filter tuned to the seventh-harmonic frequency in series with an active filter using a three-level pulsewidth modulated (PWM) converter. This paper provides a theoretical discussion on voltage-balancing control of two split dc capacitors of the active filter. The 400-V 15-kW motor drive system is designed, constructed, and tested, which can be considered as a downscaled model from a medium-voltage motor drive without regenerative braking. Experimental results verify that the hybrid filter has the capability of satisfactory harmonic filtering and stable voltage balancing in all the load conditions.
Using Deep Convolutional Networks for Gesture Recognition in American Sign Language

In the realm of multimodal communication, sign language is, and continues to be, one of the most understudied areas. In line with recent advances in the field of deep learning, there are far reaching implications and applications that neural networks can have for sign language interpretation. In this paper, we present a method for using deep convolutional networks to classify images of both the the letters and digits in American Sign Language.