Miracle-dz/UniFAR-FTUs huggingface dataset:
Live attenuated intranasal influenza vaccine

Annual vaccination is the most effective means of preventing and controlling influenza epidemics, and the traditional trivalent inactivated vaccine (TIV) is by far the most widely used. Unfortunately, it has a number of limitations, the most important of which is its poor immunogenicity in younger children and the elderly, the populations at greatest risk of severe influenza. Live attenuated influenza vaccine (LAIV) has characteristics that can overcome some of these limitations. It does not have to be injected because it is administered intranasally. It is very effective in children and adolescents, among whom it prevents significantly more cases of influenza than the traditional TIV. However, its efficacy in adults has not been adequately documented, which is why it has not been licensed for use by adults by the European health authorities. LAIV is safe and well tolerated by children aged > 2 y and adults, but some concerns arisen regarding its safety in younger children and subjects with previous asthma or with recurrent wheezing. Further studies are needed to solve these problems and to evaluate the possible role of LAIV in the annual vaccination of the general population.
Altered gene expression in hippocampus and depressive‐like behavior in young adult female mice by early protein malnutrition

Perinatal development represents a critical period in the life of an individual. A common cause of poor development is that which comes from undernutrition or malnutrition. In particular, protein deprivation during development has been shown to have deep deleterious effects on brain's growth and plasticity. Early‐life stress has also been linked with an increased risk to develop different psychopathologies later in life. We have previously shown that perinatal protein malnutrition in mice leads to the appearance of anxiety‐related behaviors in the adulthood. We also found evidence that the female offspring was more susceptible to the development of depression‐related behaviors. In the present work, we further investigated this behavior together with its molecular bases. We focused our study on the hippocampus, as it is a structure involved in coping with stressful situations. We found an increase in immobility time in the forced swimming test in perinatally malnourished females, and an alteration in the expression of genes related with neuroplasticity, early growth response 1, calcineurin and c‐fos. We also found that perinatal malnutrition causes a reduction in the number of neurons in the hippocampus. This reduction, together with altered gene expression, could be related to the increment in immobility time observed in the forced swimming test.
The impact of osteoporosis on the classification of hip and wrist fractures.

BACKGROUND Previous studies demonstrated a correlation between bone density, stability of fracture fixation, and outcome. Because current fracture classifications do not take osteoporosis into account, a prospective radiological analysis was conducted of patients with hip and wrist fracture to evaluate the impact of osteoporosis on fracture classification. MATERIAL/METHODS Altogether, 77 consecutive patients with either hip or wrist fracture were prospectively recruited within 24 hours of sustaining the fracture. The patients were assigned to subgroups according to gender, fracture site, fracture type, and bone mineral density (BMD). Using widely accepted classification systems for hip and wrist fractures, the impact of osteoporosis on fracture classification was assessed. RESULTS Osteoporosis dominated in both fracture types and bone mineral density showed a significant negative correlation with age. Pertrochanteric fractures were more frequent, showing an equal distribution among severity grades, while less frequent femoral neck fractures were mainly unstable fractures. Postmenopausal patients sustained more severe intraarticular comminuted wrist fractures, which were not found using the Fernandez classification. CONCLUSIONS The contradiction between the higher incidence but lower severity of pertrochanteric fractures compared with femoral neck fractures in osteoporotic bone and inconsistencies between the classifications of wrist fractures may indicate incomplete fracture classification in osteoporosis. Given the high incidence of osteoporotic fractures, incorporating bone mineral status into fracture classification systems may improve preoperative assessment, implant stability, and outcome.
Aging Affects the Interaction between Attentional Control and Source Memory: An fMRI Study

Age-related source memory impairments may be due, at least in part, to deficits in executive processes mediated by the PFC at both study and test. Behavioral work suggests that providing environmental support at encoding, such as directing attention toward item–source associations, may improve source memory and reduce age-related deficits in the recruitment of these executive processes. The present fMRI study investigated the effects of directed attention and aging on source memory encoding and retrieval. At study, participants were shown pictures of objects. They were either asked to attend to the objects and their color (source) or to their size. At test, participants determined if objects were seen before, and if so, whether they were the same color as previously. Behavioral results showed that direction of attention improved source memory for both groups; however, age-related deficits persisted. fMRI results revealed that, across groups, direction of attention facilitated medial temporal lobe-mediated contextual binding processes during study and attenuated right PFC postretrieval monitoring effects at test. However, persistent age-related source memory deficits may be related to increased recruitment of medial anterior PFC during encoding, indicative of self-referential processing, as well as underrecruitment of lateral anterior PFC-mediated relational processes. Taken together, this study suggests that, even when supported, older adults may fail to selectively encode goal-relevant contextual details supporting source memory performance.
Effect of acrylamide on some hormones and endocrine tissues in male rats

Acrylamide is oxidized by cytochrome P450 2E1 (CYP2E1) to its epoxide form, glycidamide, which is believed to be responsible for the mutagenic and carcinogenic activities. This study was carried out to investigate the early changes that may be related to the carcinogenic activity of acrylamide in thyroid, adrenal glands and testis in male rats. Forty adult Sprague Dawley male rats were divided equally into four groups, rats of Group I served as control, and rats of Groups II, III and IV were treated orally with acrylamide with doses 5, 10, 15 mg/kg/day body weight for 8 weeks. The results indicated that the plasma carcino embryonic antigen (CEA) and malondialdehyde (MDA) levels are higher, but free and total testosterone, triiodothyronine (T3) and thyroxine, or 3,5,3′,5′-tetraiodothyronine (T4) and corticosterone levels are lower in rats treated with acrylamide than that in control rats. This study provides evidence of endocrine disturbance to the testis, thyroid and adrenal glands, which are also the organs in which acrylamide has been shown to cause tumors in experimental animals.
Inflammatory Bowel Disease in Women: Impact on Relationship and Sexual Health

Inflammatory bowel disease (IBD) has an impact on the quality of life of women regarding partner relationships and sexual health. Partner relationship and sexual health in women with IBD has been targeted minimally for investigation in the literature devoted to psychological, relationship, and sexual functioning. The purpose of the present article is to describe the concerns of women with IBD, specifically evaluating individual concerns, partner relationships, and sexual functioning after surgery and to elucidate some of the difficulties in identifying such problems. Gynecologic issues and pregnancy concerns are described. Actual case studies are presented that reveal many of the difficulties women with IBD encounter in their relationships as a consequence of disease activity and treatment interventions. Additional research evaluating relationship difficulties, sexual comfort, and sexual behaviors as a consequence of disease activity is required to understand further and improve the quality of life and well-being of these women.
How Do Women Who Plan Home Birth Prepare for Childbirth?

In this column, the findings of a secondary analysis of data from a larger qualitative study of the experience of home birth are discussed. The aim was to describe the ways in which women who plan home birth prepare for their births. The findings provide support for the idea of birth preparation and education occurring throughout pregnancy and describe the ways in which women planning to give birth at home develop confidence, plan for support, and make decisions related to the particulars of the labor and birth. Implications of these findings for childbirth education are explored.
Sociodemographic Risk Factors and Correlates of Dementia in Older Malaysians

Objective: The rapid expansion of the aged population in Malaysia is expected to greatly increase the number of persons with dementia in the country. However, data on dementia prevalence at the national level is lacking, and little is known about the sociodemographic risk factors and correlates of dementia. This paper describes a nationwide study of dementia prevalence and its sociodemographic risk factors and health correlates among older Malaysians. Methods: In the nationwide study, the Mental Health and Quality of Life of Older Malaysians, AGECAT-GMS was used to diagnose dementia in a nationally representative sample of 2,980 persons aged 60 and above. Results: The prevalence rate of dementia was 14.3%. Higher dementia prevalences were found in oldest age (26.3%), women (19.7%), no formal education (24.1%), Bumiputeras (32.2%), unmarried (19.4%), unemployed (31.3%) and very poor on self-rated health (33.3%). Multivariate logistic regression analyses showed that older age, female gender, no formal education, ethnicity and very poor self-rated health were independent risk factors and correlates of dementia. Conclusions: Relatively higher prevalence rates of dementia in older Malaysians were accounted for by greater proportions without education, Malay and Bumiputera ethnicity, and other unknown factors which should be further investigated.
Taser Use in Restraint-Related Deaths

Objective. The Taser is an electric weapon capable of releasing significant amounts of electricity in rapid pulses, causing uncontrollable muscle contraction. Use of this weapon has dramatically increased over the past decade, andit is now commonly used by law enforcement officers nationwide. Emergency medical services providers are, likewise, seeing more patients who have recently been subjected to application of a Taser. We examined the autopsy reports of patients who died after application of a Taser in an attempt to identify high-risk interactions. Methods. This is a case series of Taser-related deaths. Fatalities occurring over four years beginning in January 2001 were identified through an Internet search, andautopsy reports were requested. Reports were analyzed for patient demographics, preexisting cardiac disease, toxicology, evidence of excited delirium, restraint techniques used, andlisted cause of death. Results. Of 75 cases identified, 37 (49.3%) had autopsy reports available for review. All cases involved men, with ages ranging from 18 to 50 years. Cardiovascular disease was found in 54.1%. Illegal substance use was found on toxicology screening for 78.4%; within that group, 86.2% were found to have been using stimulants. A diagnosis of excited delirium was given for 75.7% of the cases. Use of a Taser was considered a potential or contributory cause of death in 27%. Conclusions. This is the largest review of Taser-related fatalities reported in the medical literature. The findings are consistent with prior studies, suggesting a high frequency of restraint-related andexcited delirium–related fatalities. Key words: emergency medical services; restraint; physical; fatal outcome; law enforcement.
ADME-tox issues for the elderly.

BACKGROUND The elderly, defined as people aged >65 years, are a heterogeneous population. With increasing age there is increased prevalence of disease for which medication may be indicated and increased drug toxicity. OBJECTIVE/METHODS This review of the published scientific and clinical data on absorption, distribution, metabolism, excretion and toxicity (ADME-tox) in old age informs prescribers and drug developers of age-related factors that determine drug dose, safety and efficacy. RESULTS/CONCLUSION Increased inter-individual variability is a major feature of toxicology in old age. Changes in ADME-tox associated with normal ageing are currently better described than changes in the frail aged. Reduced hepatic and renal clearance are the most significant toxicokinetic changes seen in normal ageing. Drug toxicity in old age is influenced by changes in the quantity, affinity and responsiveness of drug targets, physiologic reserve and response to injury. Further investigation of the effects of medications on cognitive and physical functions in older adults is required.
The Ethical and Policy Implications of Research on Income Inequality and Child Well-Being

BACKGROUND AND OBJECTIVES: Child well-being is important for lifelong health and well-being. Although there is a robust evidence base linking social determinants of health (eg, relative poverty and income inequality) to child well-being, social and public health policy tends to focus on interventions to mitigate their effects, rather than remove the root causes. The goal of this study was to examine associations between child well-being and income inequality. METHODS: We compared reported rates of childhood well-being in the 2007 and 2013 UNICEF reports on child well-being in wealthy countries. Twenty indicators of child well-being (excluding child poverty) were defined consistently in both the 2007 and 2013 reports. These variables were used to create an indicator of change in child well-being over the approximate decade 2000 to 2010. For our analyses of income inequality, we used the Organization for Economic Cooperation and Development Gini coefficient of income inequality for 2009 and change between 2000 and 2009, respectively. RESULTS: The overall index of child well-being in 2013 was closely and negatively correlated with income inequality (r = −0.60, P = .004) but not with average income (r = –0.3460, P = .12). Adjustment for income inequality, children in relative poverty, and the child poverty gap did not change the lack of association between average income and child well-being in 2013 in wealthy countries. Between 2000 and 2010, child well-being scores improved most in Italy, Norway, Portugal, the United Kingdom, and Germany. The biggest declines were seen in Sweden, Canada, Japan, Switzerland, and France. Countries that experienced the largest increases in income inequality had significantly greater declines in child well-being (r = –0.51, P = .02). CONCLUSIONS: Children born into socioeconomically disadvantaged families suffer worse child well-being and its lifelong implications, in all societies, worldwide. Our analyses show, however, that some wealthy societies are able to mitigate these inequalities; these societies have better child well-being, on average. This outcome has less to do with specific welfare policies or targeted interventions for poor children than to a societal commitment to greater equality.
Extended cusp-like regions and their dependence on the Polar orbit, seasonal variations, and interplanetary conditions

[1] Extended cusp-like regions (ECRs) are surveyed, as observed by the Magnetospheric Ion Composition Sensor (MICS) of the Charge and Mass Magnetospheric Ion Composition Experiment (CAMMICE) instrument aboard Polar between 1996 and 1999. The first of these ECR events was observed on 29 May 1996, an event widely discussed in the literature and initially thought to be caused by tail lobe reconnection due to the coinciding prolonged interval of strong northward IMF. ECRs are characterized here by intense fluxes of magnetosheath-like ions in the energy-per-charge range of ∼1 to 10 keV e−1. We investigate the concurrence of ECRs with intervals of prolonged (lasting longer than 1 and 3 hours) orientations of the IMF vector and high solar wind dynamic pressure (PSW). Also investigated is the opposite concurrence, i.e., of the IMF and high PSW with ECRs. (Note that these surveys are asking distinctly different questions.) The former survey indicates that ECRs have no overall preference for any orientation of the IMF. However, the latter survey reveals that during northward IMF, particularly when accompanied by high PSW, ECRs are more likely. We also test for orbital and seasonal effects revealing that Polar has to be in a particular region to observe ECRs and that they occur more frequently around late spring. These results indicate that ECRs have three distinct causes and so can relate to extended intervals in (1) the cusp on open field lines, (2) the magnetosheath, and (3) the magnetopause indentation at the cusp, with the latter allowing magnetosheath plasma to approach close to the Earth without entering the magnetosphere.
Salt‐losing nephropathy in mice with a null mutation of the Clcnk2 gene

The basolateral chloride channel ClC‐Kb facilitates Cl reabsorption in the distal nephron of the human kidney. Functional mutations in CLCNKB are associated with Bartter's syndrome type 3, a hereditary salt‐losing nephropathy. To address the function of ClC‐K2 in vivo, we generated ClC‐K2‐deficient mice.
Herpes Simplex Virus DNA Cleavage and Packaging Proteins Associate with the Procapsid prior to Its Maturation

ABSTRACT Packaging of DNA into preformed capsids is a fundamental early event in the assembly of herpes simplex virus type 1 (HSV-1) virions. Replicated viral DNA genomes, in the form of complex branched concatemers, and unstable spherical precursor capsids termed procapsids are thought to be the substrates for the DNA-packaging reaction. In addition, seven viral proteins are required for packaging, although their individual functions are undefined. By analogy to well-characterized bacteriophage systems, the association of these proteins with various forms of capsids, including procapsids, might be expected to clarify their roles in the packaging process. While the HSV-1 UL6, UL15, UL25, and UL28 packaging proteins are known to associate with different forms of stable capsids, their association with procapsids has not been tested. Therefore, we isolated HSV-1 procapsids from infected cells and used Western blotting to identify the packaging proteins present. Procapsids contained UL15 and UL28 proteins; the levels of both proteins are diminished in more mature DNA-containing C-capsids. In contrast, UL6 protein levels were approximately the same in procapsids, B-capsids, and C-capsids. The amount of UL25 protein was reduced in procapsids relative to that in more mature B-capsids. Moreover, C-capsids contained the highest level of UL25 protein, 15-fold higher than that in procapsids. Our results support current hypotheses on HSV DNA packaging: (i) transient association of UL15 and UL28 proteins with maturing capsids is consistent with their proposed involvement in site-specific cleavage of the viral DNA (terminase activity); (ii) the UL6 protein may be an integral component of the capsid shell; and (iii) the UL25 protein may associate with capsids after scaffold loss and DNA packaging, sealing the DNA within capsids.
Interleukin‐1 family cytokines and their regulatory proteins in normal pregnancy and pre‐eclampsia

Maternal systemic inflammation is a feature of pre‐eclampsia, a condition in pregnancy characterized by hypertension and proteinuria. Pre‐eclampsia is caused by the placenta; many placental factors contribute to the syndrome's progression, and proinflammatory cytokines have been identified previously as one such mediator. The interleukin (IL)‐1 family of cytokines are key regulators of the inflammatory network, and two naturally occurring regulatory molecules for IL‐1 family cytokines, IL‐1RA and sST2, have been found previously to be elevated in maternal blood from women with pre‐eclampsia. Here we investigate more recently identified IL‐1 family cytokines and regulatory molecules, IL‐1RAcP, IL‐37, IL‐18BP, IL‐36α/β/γ/Ra and IL‐38 in pre‐eclampsia. Pregnant women have more circulating IL‐18BP and IL‐36Ra than non‐pregnant women, and sIL‐1RAcP is elevated from women with pre‐eclampsia compared to normal pregnancies. The placenta expresses all the molecules, and IL‐37 and IL‐18BP are up‐regulated significantly in pre‐eclampsia placentas compared to those from normal pregnancies. Together, these changes contribute to the required inhibition of maternal systemic cytotoxic immunity in normal pregnancy; however, in pre‐eclampsia the same profile is not seen. Interestingly, the increased circulating levels of sIL‐1RAcP and increased placental IL‐18BP and IL‐37, the latter of which we show to be induced by hypoxic damage to the placenta, are all factors which are anti‐inflammatory. While the placenta is often held responsible for the damage and clinical symptoms of pre‐eclampsia by the research community, here we show that the pre‐eclampsia placenta is also trying to prevent inflammatory damage to the mother.
Optimized Multidetector Computed Tomographic Protocol for the Diagnosis of Active Obscure Gastrointestinal Bleeding: A Feasibility Study

Objective: The purpose of this feasibility study was to prospectively evaluate an optimized multidetector computed tomographic protocol for the diagnosis of active obscure gastrointestinal bleeding (OGIB). Methods: Between October 2006 and February 2008, patients admitted for active OGIB were included in this prospective unicenter study. Water was administered orally and rectally as neutral luminal contrast material. A contrast-enhanced 16-row multidetector computed tomography (MDCT) was performed in the arterial and venous phases. Mesenteric digital subtraction angiography was carried out immediately after MDCT as standard of reference. Results: Six patients were included in this study. Multidetector computed tomography identified the bleeding site and source in 5 (83%) of the patients. Digital subtraction angiography was performed in 4 patients, and the result was positive in 1 (25%) of the patients. Multidetector computed tomography detected the site and source of bleeding in 2 patients whose digital subtraction angiographic result was negative. Conclusions: The results of this feasibility study indicate that optimized MDCT is an excellent diagnostic tool for the diagnosis of active OGIB.
Magnetic Resonance-guided Prostate Interventions

Abstract: We review our experience using an open 0.5-T magnetic resonance (MR) interventional unit to guide procedures in the prostate. This system allows access to the patient and real-time MR imaging simultaneously and has made it possible to perform prostate biopsy and brachytherapy under MR guidance. We review MR imaging of the prostate and its use in targeted therapy, and describe our use of image processing methods such as image registration to further facilitate precise targeting. We describe current developments with a robot assist system being developed to aid radioactive seed placement.