Miracle-dz/UniFAR-FTUs huggingface dataset:
The JAL antigen (RH48) is the result of a change in RHCE that encodes Arg114Trp

BACKGROUND: The JAL antigen (Rh48) was discovered more than 30 years ago when it caused hemolytic disease of the fetus and newborn in an African American family. A decade later it was found to cause hemolytic disease of the fetus and newborn in a Caucasian family. The presence of the same low‐prevalence antigen in two different ethnic groups is rare, but additional JAL+ in both groups was subsequently identified. This study was undertaken to investigate the RH gene(s) responsible for expression of JAL and to determine the structural relationship between JAL and other Rh antigens.
Technology-Enhanced Program for Child Disruptive Behavior Disorders: Development and Pilot Randomized Control Trial

Early onset disruptive behavior disorders are overrepresented in low-income families; yet these families are less likely to engage in behavioral parent training (BPT) than other groups. This project aimed to develop and pilot test a technology-enhanced version of one evidence-based BPT program, Helping the Noncompliant Child (HNC). The aim was to increase engagement of low-income families and, in turn, child behavior outcomes, with potential cost-savings associated with greater treatment efficiency. Low-income families of 3- to 8-year-old children with clinically significant disruptive behaviors were randomized to and completed standard HNC (n = 8) or Technology-Enhanced HNC (TE-HNC; n = 7). On average, caregivers were 37 years old; 87% were female, and 80% worked at least part-time. More than half (53%) of the youth were boys; the average age of the sample was 5.67 years. All families received the standard HNC program; however, TE-HNC also included the following smartphone enhancements: (a) skills video series, (b) brief daily surveys, (c) text message reminders, (d) video recording home practice, and (e) midweek video calls. TE-HNC yielded larger effect sizes than HNC for all engagement outcomes. Both groups yielded clinically significant improvements in disruptive behavior; however, findings suggest that the greater program engagement associated with TE-HNC boosted child treatment outcome. Further evidence for the boost afforded by the technology is revealed in family responses to postassessment interviews. Finally, cost analysis suggests that TE-HNC families also required fewer sessions than HNC families to complete the program, an efficiency that did not compromise family satisfaction. TE-HNC shows promise as an innovative approach to engaging low-income families in BPT with potential cost-savings and, therefore, merits further investigation on a larger scale.
Organization of the Human Gene Encoding the Epididymis-Specific EP2 Protein Variants and Its Relationship to Defensin Genes1

Abstract The EP2 gene codes for at least nine message variants that are all specifically expressed in the epididymis. These variants putatively encode small secretory proteins that differ in their N- and C-termini, resulting in proteins that can have little or no sequence similarity to each other. We have isolated and sequenced the human EP2 gene to determine the molecular origin of these variants. The EP2 gene has two promoters, eight exons, and seven introns. Exons 3 and 6 encode protein sequences homologous to β-defensins, a family of antimicrobial peptides. This sequence homology and the arrangement of promoters and defensin-encoding exons suggest that the EP2 gene originated from two ancestral β-defensin genes arranged in tandem, each contributing a promoter and two exons encoding a leader sequence and a defensin peptide. The proposed evolutionary relationship between the EP2 gene and defensin genes is supported by the observation that the EP2 gene is located on chromosome 8p23 near the defensin gene cluster and is separated by 100 kilobases or less from DEFB2, the gene for β-defensin-2. While the EP2 gene transcribes β-defensin-like message variants, most of the known message variants code for nondefensin proteins or proteins containing only a partial defensin peptide sequence. We suggest that, during its evolution, the EP2 gene has acquired new functions that may be important for sperm maturation and/or storage in the epididymis.
Robust Inference of Neuronal Correlations from Blurred and Noisy Spiking Observations

Emerging large-scale neuronal recording technolo-gies, such as two-photon calcium imaging, typically provide blurred and noisy surrogates of spiking activity. Extracting the underlying neuronal correlations, which are key to understanding neural function and circuitry, from such data is thus a challenging task. Though deconvolution techniques are often applied to such data to recover spiking activity, they require high temporal resolution and signal-to-noise ratio conditions to be effective. In addition, their solutions are biased towards obtaining accurate first-order statistics (i.e., spike detection) via spatiotemporal priors, which may be detrimental to recovering second-order statistics (i.e., correlations). Existing methods for inferring neuronal correlations from two-photon data thus suffer from significant bias and variability. In this work, we propose an algorithm to directly estimate neuronal correlations from ensemble two-photon imaging data, by integrating techniques from point process modeling and variational Bayesian inference, with no recourse to intermediate spike deconvolution. We demonstrate through simulation studies that the proposed method outperforms existing approaches in accurately capturing the underlying neuronal correlations.
Management of hearing loss in Apert syndrome

Background: Apert syndrome is one of the craniosynostosis syndromes, with abirth prevalence estimated to be between 9.9 and 15.5/million, and accounts for 4.5 per cent ofcraniosynostoses. Although conductive hearing loss is common in Apert syndrome there are contradicting reports regarding the cause of this hearing loss. There is also no detailed informationavailable on the management of hearing loss in Apert syndrome. Materials and methods: A retrospective analysis of case notes of Apert syndrome patients seen between 1970 and 2003 at Great Ormond Street Children’s Hospital, London, was undertaken. Results: Seventy case notes were obtained. The incidence of congenital hearing impairment was between 3 and 6 per cent. Almost all patients had otitis media with effusion (glue ear), which tended to persist into adult life. More than 56 per cent of cases developed permanent conductive hearing loss by 10–20 years. Repeated grommet insertion was common; even though 35 per cent had trouble with ear discharge and persistent conductive hearing loss. Statistically, grommets made no difference to the risk of developing permanent hearing loss. Conclusion:This study, of the largest number of Apert syndrome cases assembled to date, showed that early optimization of hearing with possible hearing aids needs to be considered. Repeated grommet insertion does not help in optimizing hearing, especially if ear discharge complicates the picture.
Eilatimonas milleporae gen. nov., sp. nov., a marine bacterium isolated from the hydrocoral Millepora dichotoma.

A marine bacterial strain, designated MD2(T), was isolated from the damaged tissue of a hydrocoral, Millepora dichotoma, collected from the coral reef in the northern Red Sea, Gulf of Eilat, Israel. Strain MD2(T) was Gram-reaction-negative, rod-shaped and motile, and formed small, creamy and opaque colonies, 1-2 mm in diameter, after 3 days incubation on Marine agar at 30°C. The novel strain grew well in nutrient broth at 1.5-6 % NaCl and at 20-37°C. The major cellular fatty acids were iso-C17 : 1ω9c, iso-C17 : 0, C18 : 1ω7c and C17 : 1ω6c. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, an unidentified lipid, two unidentified phospholipids, two unidentified glycolipids and two unidentified aminolipids. Ubiquinone Q-10 was the only respiratory lipoquinone. The DNA G+C content was 60.3 mol%. Analysis of the 16S rRNA gene sequence placed the organism in the α-subclass of the Proteobacteria with a sequence divergence of about 9 % from any species with a validly published name. The highest 16S rRNA gene sequence similarity (approximately 91 %) was notably with type strains of members of the genus Kordiimonas, Kordiimonas aestuarii 101-1(T), Kordiimonas lacus S3-22(T) and Kordiimonas gwangyangensis GW14-5(T). On the basis of genotypic, chemotaxonomic and phenotypic distinctness, strain MD2(T) represents a novel species in a new genus of the class Alphaproteobacteria, for which the name Eilatimonas milleporae gen. nov., sp. nov. is proposed. The type strain of the type species is MD2(T) ( = LMG 26586(T) = DSM 25217(T)).
Chemical composition and biological activities of Jeju Thymus quinquecostatus essential oils against Propionibacterium species inducing acne.

The genus Propionibacterium includes common Gram-positive, non-spore forming, microaerophilic and rod-shaped bacteria. These organisms are classifi ed as classical and cutaneous propionibacteria. Classical propionibacteria are used to make dairy foods and to produce vitamin B12, tetra pyrolle compounds, and propionic acids. Members of the cutaneous propionibacteria include P. acnes, P. granulosum and P. propionicum (Cheong et al., 2008; Kiatpapan and Murooka, 2002). Among them, P. acnes and P. granulosum can cause several infections such as acne vulgaris, chronic endophthalmitis and uveitis (Cheong et al., 2008; Csukas et al., 2004). Acne vulgaris is the most common cutaneous disorder. P. acnes produces a number of virulence factors and is well known for its infl ammatory and immunomodulatory properties (Kim, 2005; Perry and Lambert, 2006). The main components of the pilosebaceous unit of the skin, such as keratinocytes and sebocytes, can be activated by P. acnes, leading to the production of pro-infl ammatory cytokines (Bojar and Holland, 2004; Farrar and Ingham, 2004; Jain and Basal, 2004). Many topical and systemic treatment methods have been proposed for the treatment of acne vulgaris. Retinoids are most commonly used to treat acne, but their main side effect is skin irritation (Thielitz et al., 2006). Clindamycin and erythromycin are the most frequently used topical anti-P. acnes agents. These topical antibiotics reduce the population of P. acnes and exert anti-infl ammatory activity, but feature the major disadvantage of dramatically increasing bacterial resistance. Extensive use of antibiotics to treat acne has built up widespread resistance in cutaneous propionibacterium (Ergin et al., 2006; Guay, 2007; Lim et al., 2007; Tanghetti, 2007). A new approach for preventing antibiotic resistance is the use of essential oil therapy. Although trials have shown that treatment with essential oil prevented the emergence of resistance and sometimes enhanced the synergic effects, some limitations remain in essential oil treatment (Loughlin et al., 2008; Shahverdi et al., 2007; Si et al., 2008). Thus, further studies of essential oils are needed. The genus Thymus includes ornamental, usually arJ. Gen. Appl. Microbiol. , 55, 63‒68 (2009)
Is therapeutic judgement influenced by the patient’s socio-economic status? A factorial vignette survey

Abstract Background In Denmark patients are entitled to rehabilitation regardless of socio-economic status (SES). During this process therapists have to balance cost effectiveness with providing equal treatment. Aim To investigate whether occupational therapists and physiotherapists were influenced by the patient’s SES. Material and method An experimental factorial vignette survey was used. Four different vignettes describing fictitious patient cases with different SES variables were randomly allocated to therapists working in somatic hospitals. Thereafter, the therapists judged specific clinical situations and general attitudes in relation to the patient’s SES. Chi-square was used to test the statistical association between the variables. Results No statistically significant associations were found between the specific clinical situations and the patient’s SES. A statistical significant association was found between general attitudes and the patient’s SES. Subgroup analysis revealed a statistically significant association between the therapist’s gender, age, and the therapeutic judgement in relation to SES. Conclusion In the specific clinical situations, Danish therapists seem to maintain their professional ethical principles, although they might face ethical dilemmas during their clinical decision-making. In order to prevent and resolve these dilemmas, they have to be made explicit. However, further research on how SES influences the health care professional’s judgement is warranted.
Prevention of Type I Diabetes in NOD Mice by Adjuvant Immunotherapy

The nonobese diabetic (NOD) mouse is an excellent model of insulin-dependent (type I) human diabetes mellitus. We report that a single injection of complete Freund's adjuvant (CFA) given at an early age (5 wk) prevented the appearance of diabetes and greatly increased the life span of NOD mice without additional therapy. No treated mouse developed hyperglycemia by the age of 12 mo (n = 13), whereas all untreated mice died of diabetes before 8 mo of age (n = 38). All CFA-treated mice were alive and healthy at 12 mo of age. Some CFA-treated NOD mice that were monitored for long-term survival are still alive with no sign of disease at 18 mo of age (n = 5). Administration of CFA resulted in decreased in vitro splenic lymphocyte proliferative responses to alloantigen and mitogen. Cell-mixing experiments indicated that antigen-nonspecific inhibitory cells were elicited in the spleen and increased in the bone marrow. These regulatory cells were Thy-1− ; and nonadherent to nylon wool, as has been described for natural suppressor (NS) cells. These data lend support to a relationship between the boosting of endogenous NS activity and the establishment of tolerance to self in the context of autoimmunity. Our results suggest that early nonspecific immunotherapy of genetically predisposed individuals could prevent the development of autoimmune diabetes.
Lymphatic drainage of Listeria inonocytogenes and Indian ink inoculated in the peritoneal cavity of the mouse

The lymphatic drainage of the peritoneal cavity has been investigated by intraperitoneal inoculation of an intracellular bacterium (Listeria monocytogenes) and an inert marker (Indian ink). The results reveal that both agents are transported, either after phagocytosis by intraperitoneal macro phages or in suspension in the lymph, towards the cranial sternal lymph nodes (Lymphonodi sternales craniales) of the ventral thoracic Iymphocentrum (Lymphocentrum thoracicum ventrale) and to the lymph nodes of the mediastinal lymphocentrum (Lymphocentrum mediastinale), prior to systemic dissemination. This mechanism of peritoneal lymph drainage has relevance on experimental studies involving the inoculation of pathogens, and on the investigation of metastatic diffusion of neoplasms from the peritoneum.
Laparoscopy and colon cancer. Is the port site at risk? A preliminary report.

PURPOSE To quantify the magnitude of the risk for port/extraction site recurrence of laparoscopically resected colon cancer in a defined study population. METHODS The data from a prospective laparoscopic bowel surgery registry was used to identify cases of colon cancer that were resected laparoscopically, with a minimum follow-up of 1 year. A questionnaire was sent to the surgeons who performed the procedures. RESULTS A total of 252 cases were identified from the registry. Questionnaires were returned in 208 of those cases, a response rate of 82.5%. Three cases of port or extraction site recurrence were noted, two of them associated with diffuse peritoneal carcinomatosis. All the patients had a Dukes' stage C tumor at the time of initial surgery. CONCLUSIONS The incidence of port/extraction site recurrence following laparoscopic colon cancer surgery is low. All the recurrences were in patients with Dukes' stage C tumors, and there was diffuse peritoneal carcinomatosis in two of the three cases, suggesting that port/extraction site recurrence may be attributable to the advanced nature of the disease rather than the laparoscopic technique. Longer follow-up and more cases are required to confirm these findings.
A Comprehensive Review of Metasurface Structures Suitable for RF Energy Harvesting

Energy harvesting (EH) or scavenging is recognized as harvesting energy from ambient energy sources in the surrounding environment. This paper reports a literature review on radio frequency (RF) EH using different metasurface/metamaterial structures based on split-ring resonators (SRRs), electric inductive–capacitive (ELC) resonators, square-patch unit cells, square-ring unit cells, etc. The essential parameters in rectifying antenna (rectenna) design are included, such as receiving antenna efficiency, conversion efficiency, dimensions, supporting substrate properties, frequency band, and overall performance, etc. It is noted that rectenna design using conventional antennas such as microstrip antennas, monopole antennas, slot antennas, dielectric resonator antennas, etc. suffers from low power conversion efficiency with larger size. To overcome the above-mentioned constraints and enhance the conversion efficiency with smaller size, metasurface/metamaterial structures are used as EH collectors. An introduction to EH is discussed, followed by an overview of energy sources in the ambient environment. Several hypothetical and experimental studies on metasurface-based EH systems are summarized.
Alkylglycerol Prodrugs of Phosphonoformate Are Potent In Vitro Inhibitors of Nucleoside-Resistant Human Immunodeficiency Virus Type 1 and Select for Resistance Mutations That Suppress Zidovudine Resistance

ABSTRACT Phosphonoformate (foscarnet; PFA) is a potent inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), but its use for the treatment of HIV-1 infection is limited by toxicity and the lack of an orally bioavailable formulation. Alkylglycerol-conjugated prodrugs of PFA (1-O-octadecyl-sn-glycero-3-PFA [B-PFA]) having sn-2 substituents of hydrogen (deoxybatyl-PFA [DB-PFA]), methyl (MB-PFA), or ethyl (EB-PFA) are more-potent inhibitors of wild-type HIV-1 in vitro than unmodified PFA and are orally bioavailable in mice. We have evaluated the activities of these compounds against a panel of nucleoside-resistant HIV-1 variants and have characterized the resistant variants that emerge following in vitro selection with the prodrugs. Except for an HIV-1 variant encoding the K65R mutation in RT that exhibited 3.3- to 8.2-fold resistance, the nucleoside-resistant viruses included in the panel were sensitive to the PFA prodrugs (<3-fold increase in 50% inhibitory concentration), including multinucleoside-resistant variants encoding the Q151M complex of mutations or the T69S[SA] insert. Viruses resistant to the PFA prodrugs (>10-fold) were selected in vitro after 15 or more serial passages of HIV-1 in MT-2 cells in escalating prodrug concentrations. Mutations detected in the resistant viruses were S117T, F160Y, and L214F (DB-PFA); M164I and L214F (MB-PFA); and W88G and L214F (EB-PFA). The S117T, F160Y, and M164I mutations have not been previously identified. Generation of recombinant viruses encoding the single and double mutations confirmed their roles in prodrug resistance, including 214F, which generally increased the level of resistance. When introduced into a zidovudine (AZT)-resistant background (67N 70R 215F 219Q), the W88G, S117T, F160Y, and M164I mutations reversed AZT resistance. This suppression of AZT resistance is consistent with the effects of other foscarnet resistance mutations that reduce ATP-dependent removal of AZT monophosphate from terminated template primers. The favorable activity and resistance profiles of these PFA prodrugs warrant their further evaluation as clinical candidates.
Theory of Mind and Executive Function: Working-Memory Capacity and Inhibitory Control as Predictors of False-Belief Task Performance

This study of the relationship between theory of mind and executive function examined whether on the false-belief task age differences between 3 and 5 years of age are related to development of working-memory capacity and inhibitory processes. 72 children completed tasks measuring false belief, working memory, and inhibition. Significant age effects were observed for false-belief and working-memory performance, as well as for the false-alarm and perseveration measures of inhibition. A simultaneous multiple linear regression specified the contribution of age, inhibition, and working memory to the prediction of false-belief performance. This model was significant, explaining a total of 36% of the variance. To examine the independent contributions of the working-memory and inhibition variables, after controlling for age, two hierarchical multiple linear regressions were conducted. These multiple regression analyses indicate that working memory and inhibition make small, overlapping contributions to false-belief performance after accounting for age, but that working memory, as measured in this study, is a somewhat better predictor of false-belief understanding than is inhibition.
Selection and Genetic Characterization of Streptococcus pneumoniae Mutants Resistant to the Des-F(6) Quinolone BMS-284756

ABSTRACT Existing quinolones are known to target the type II topoisomerases in bacteria. In order to determine which of these targets are of key importance in Streptococcus pneumoniae treated with BMS-284756 (T-3811ME), a novel des-F(6) quinolone, resistant mutants were selected in several steps of increasing resistance by plating pneumococci on a series of blood agar plates containing serial twofold-increasing concentrations of drug. After incubation, colonies that arose were selected and passaged twice on antibiotic-containing media at the selection level. Mutants generally showed increases in resistance of four- to eightfold over the prior level of susceptibility. Mutants in the next-higher level of resistance were selected from the previous round of resistant mutants. Subsequently, chromosomal DNA was prepared from parental (R6) pneumococci and from at least three clones from each of four levels of increasing antibiotic resistance. Using PCR primers, 500- to 700-bp amplicons surrounding the quinolone resistance determining regions (QRDR) ofgyrA, gyrB, parC, andparE genes were prepared from each strain. Internal primers were used to sequence both DNA strands in the regions of approximately 400 bp centered on the QRDR. Mutations identified with increasing levels of resistance included changes in GyrA at Ser-81 and Glu-85 and changes in ParC at Ser-79 and Asp-83. Changes in GyrB and ParE were not observed at the levels of resistance obtained in this selection. The resistance to comparator quinolones (levofloxacin, ciprofloxacin, and moxifloxacin) also increased in four- to eightfold steps with these mutations. The intrinsically greater level of antibacterial activity and thus lower MICs of BMS-284756 observed at all resistance levels in this study may translate to coverage of these resistant pneumococcal strains in the clinic.