Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional dissociation of adult‐born neurons along the dorsoventral axis of the dentate gyrus

Adult‐born granule cells in the mammalian dentate gyrus have long been implicated in hippocampal dependent spatial learning and behavioral effects of chronic antidepressant treatment. Although recent anatomical and functional evidence indicates a dissociation of the dorsal and ventral regions of the hippocampus, it is not known if adult neurogenesis within each region specifically contributes to distinct functions or whether adult‐born cells along the entire dorsoventral axis are required for these behaviors. We examined the role of distinct subpopulations of adult‐born hippocampal granule cells in learning‐ and anxiety‐related behaviors using low‐dose focal x‐irradiation directed specifically to the dorsal or ventral dentate gyrus. Our findings indicate a functional dissociation between adult‐born neurons along the longitudinal axis of the dentate gyrus wherein new neurons in the dorsal dentate gyrus are required for timely acquisition of contextual discrimination while immature neurons in the ventral dentate gyrus are necessary for anxiolytic/antidepressant‐related effects of fluoxetine. Interestingly, when contexts are presented with altered temporal cues, or fluoxetine is administered alongside chronic glucocorticoid treatment, this dissociation is abrogated such that adult‐born neurons across the entire dorsoventral extent of the dentate gyrus appear to contribute to these behaviors. Our results suggest that individual subpopulations of adult‐born hippocampal neurons may be sufficient to mediate distinct behaviors in certain conditions, but are required to act in concert in more challenging situations. © 2014 Wiley Periodicals, Inc.
Offering free NRT through a tobacco quitline: impact on utilisation and quit rates

Objective: To assess the impact on quitline utilisation and cessation outcomes of adding free nicotine patches to the existing programme offerings. Methods: Tobacco use status data from the Ohio tobacco quitline were collected from a subset of quitline callers 6 months after the initial intake call. To evaluate the impact of the nicotine replacement therapy (NRT) initiative, quit rates for two groups were compared: those who entered and exited the quitline programme before the availability of free NRT (n = 4657) and those who entered and exited the quitline programme after the availability of free NRT (n = 5715). Results: Call volume increased from 2351 intakes calls per month or 78 calls per day before the availability of free NRT to 3606 intake calls per month or 188 intakes per day following the availability of free NRT (p<0.0001). 7-day point prevalence abstinence at 6 months among all quitline callers increased from 10.3% (95% confidence interval (CI) 9.7 to 10.9) before the availability of NRT to 14.9% (95% CI 14.3 to 15.5) after the availability of NRT. Conclusion: Offering free NRT through a state quitline is an effective means of increasing quitline utilisation and improving quit rates.
TeO/sub 2/-based glasses containing Nb/sub 2/O/sub 5/, TiO/sub 2/, and WO/sub 3/ for discrete Raman fiber amplification

We describe a highly stable glass family based on the combination of TeO/sub 2/ with heavy metal-oxides of Nb, Ti, W. We show that these glasses have large Raman gain, a broad Raman spectrum, and large negative dispersion. They are, thus, potentially useful in a variety of specialty fiber applications, such as discrete Raman amplification.
Metabolism of trans-Aconitic Acid in Maize : I. PURIFICATION OF TWO MOLECULAR FORMS OF CITRATE DEHYDRASE.

Trans-aconitate synthesis via citrate dehydrase was determined in crude extracts of maize (Zea mays L.) coleoptiles. Two molecular forms of this enzyme were purified by substrate-specific elution from DEAE-cellulose, ammonium sulfate precipitation, and gel filtration. Each molecular form migrates as a single band in isoelectric focusing. Gel filtration and sodium dodecyl sulfate electrophoresis provided evidence that one enzyme form is composed of four 80,000-dalton subunits while the other is composed of two 60,000-dalton subunits. There was no evidence of proteolytic conversion of the large to the small molecular weight form when the former was incubated with either the 15,000(g) supernatant or with proteases. The data indicate that the two molecular forms of citrate dehydrase are isozymes.
Perennial transmission of malaria by the Anopheles gambiae complex in a North Sudan Savanna area of Mali

In Mali, West Africa, the Anopheles gambiae complex of mosquitoes is composed of Anopheles arabiensis Patton and three chromosomal forms of Anopheles gambiae Giles sensu stricto named Bamako, Savanna and Mopti (Tour6 et al., 1983, 1984; Coluzzi et al., 1985). These four types of An.gambiae sensu lato show remarkable differences of spatial and seasonal distributions (Coluzzi et al., 1985; Tour6 et al., 1984, 1987, 1994). They are all competent (i.e. susceptible) vectors of malaria and Bancroftian filariasis (Tour6 et al., 1986). but their relative contributions to the transmission of these infections are poorly understood. This study assesses, under field conditions, factors influencing the vector population biology and structure and the malaria transmissiqn pattern in an area where An.gambiae Mopti form predominates and malaria is hyperendemic. The study area was the village of Douna (13" 13' latitude north, 05'35' longitude west) with more than 700 inhabitants, located 285 km northeast of Bamako, near a flooded area on the banks of the Bani River (a tributary of the Niger River) in the North Sudan Savanna zone of Mali. The rainy season is concentrated in less than 70 days from June to September and mean annual rainfall is about 700 mm. During the dry season, the sandy bed of the Bani River contains pockets of water which allow mosquito larval breeding, especially of the An.gambiae complex. Entomological and parasitological studies were conducted during 2 weeks in October 1988 (end of the rainy season) and 2 weeks in April 1989 (dry season). Mosquito collections were made by pyrethrum spray catch (PSC) in human dwellings. The man-biting rate (MBR) was calculated from spray catches as the geometric mean of the numbers of fed plus half-gravid female mosquitoes per house, divided by the average number of sleepers per house. Polytene chromosomes of ovarian nurse cells were examined to identify sibling species of the An. gambiae complex and to assess their inversion polymorphism (Hunt, 1973; Coluzzi, 1979). To determine the human blood index (anthropophilic rate),
Myocardial remodelling: pharmacological targets

Despite considerable progress in therapy, the progressive augmentation of lifespan makes cardiac remodelling and its consequence, heart failure, a major cause of mortality and morbidity. Heart failure is consequently becoming a major goal in pharmacological research. New approaches include converting enzyme inhibitors, β-blockers and anti-aldosterones and have demonstrated that cardiac remodelling is, at least partly, a reversible process. This review aims to establish a strategy for therapeutic research which is based on the recent advances on the molecular mechanisms of cardiac remodelling, and also to evaluate some of the new developments which are presently in progress, including new inotropic drugs, new receptors or signals blockers, nitric oxide donors, metalloproteinases and apoptotic inhibitors. Our view is clearly evolutionary and several of our conclusions may contradict current opinions, such as those which consider hypertrophy a detrimental process, hormones as a primary cause of cardiac remodelling or inotropic interventions as beneficial.
Dexamethasone Represses Signaling through the Mammalian Target of Rapamycin in Muscle Cells by Enhancing Expression of REDD1*

The mammalian target of rapamycin (mTOR), a critical modulator of cell growth, acts to integrate signals from hormones, nutrients, and growth-promoting stimuli to downstream effector mechanisms involved in the regulation of protein synthesis. Dexamethasone, a synthetic glucocorticoid that represses protein synthesis, acts to inhibit mTOR signaling as assessed by reduced phosphorylation of the downstream targets S6K1 and 4E-BP1. Dexamethasone has also been shown in one study to up-regulate the expression of REDD1 (also referred to RTP801, a novel stress-induced gene linked to repression of mTOR signaling) in lymphoid, but not nonlymphoid, cells. In contrast to the findings of that study, here we demonstrate that REDD1, but not REDD2, mRNA expression is dramatically induced following acute dexamethasone treatment both in rat skeletal muscle in vivo and in L6 myoblasts in culture. In L6 myoblasts, the effect of the drug on mTOR signaling is efficiently blunted in the presence of REDD1 RNA interference oligonucleotides. Moreover, the dexamethasone-induced assembly of the mTOR regulatory complex Tuberin·Hamartin is disrupted in L6 myoblasts following small interfering RNA-mediated repression of REDD1 expression. Finally, overexpression of Rheb, a downstream target of Tuberin function and a positive upstream effector of mTOR, reverses the effect of dexamethasone on phosphorylation of mTOR substrates. Overall, the data support the conclusion that REDD1 functions upstream of Tuberin and Rheb to down-regulate mTOR signaling in response to dexamethasone.
Fiber and Cardiovascular Disease Risk: How Strong Is the Evidence?

Dietary fiber consists of edible parts of plants or analogous carbohydrates that are resistant to digestion and absorption in the human small intestine. Fiber can be classified as a dietary source (eg, cereal, fruit, vegetable, or legume) or as a supplement. Based on chemical properties, fiber can be divided to water-soluble (eg, &bgr;-glucans, pectin, and guar) and insoluble (eg, cellulose and lignin) forms. An increasing number of observational findings have reported a lower incidence of coronary heart disease in subjects who report consuming diets high in fiber. Dietary fiber is thought to affect several cardiovascular disease (CVD) risk factors. Soluble fiber decreases serum total and low-density lipoprotein cholesterol concentrations and improves insulin resistance. The effect of fiber on inflammatory markers and coagulation is not yet well established. While soluble, gel-forming fiber has beneficially affected CVD risk factors, food sources of mainly insoluble fibers, primarily contributed by cereal products, have been the most consistently associated with lower incidence rates of CVD. Despite this contradiction, the evidence promotes a food-based approach favoring increased intake of whole-grain cereals, fruit, and vegetables providing a mixture of different types of fibers for CVD prevention.
Use of Epidemiologically Well-Defined Subjects and Existing Immunofluorescence Assays To Calibrate a New Enzyme Immunoassay for Human Herpesvirus 8 Antibodies

ABSTRACT Agreement between assays for the detection of human herpesvirus 8 (HHV-8) antibodies has been limited. In part, this disagreement has been because assay calibration (i.e., differentiating positive from negative results) has not been done in a standardized fashion with reference to a wide spectrum of HHV-8-infected (true-positive) and HHV-8-uninfected (true-negative) persons. To describe the performance of an assay for HHV-8 antibodies more accurately, we used epidemiologically well-characterized subjects in conjunction with testing on two existing immunofluorescence assays for HHV-8 antibodies to define two groups: a group of 135 HHV-8-infected individuals (true positives), including Kaposi's sarcoma patients and those asymptomatically infected, and a group of 234 individuals with a high likelihood of being HHV-8 uninfected (true negatives). A new enzyme immunoassay (EIA), using lysed HHV-8 virion as the antigen target, was then developed. With the above true positives and true negatives as references, the sensitivity and specificity of the EIA associated with different cutoff values were determined. At the cutoff that maximized both sensitivity and specificity, sensitivity was 94% and specificity was 93%. When the EIA was used to test a separate validation group, a distribution of seropositivity that matched that predicted for the agent of Kaposi's sarcoma was observed: 55% of homosexual men were seropositive, versus 6% seropositivity in a group of children, women, and heterosexual men. It is proposed that the EIA has utility for large-scale use in a number of settings and that the calibration method described can be used for other assays, both to more accurately describe the performance of these assays and to permit more-valid interassay comparison.
Comparative Evaluation of Methods for Attributing Responsibilities Due To Voltage Unbalance

This paper presents the results of a comparative analysis among three methods for attributing responsibilities of voltage unbalance in the electrical network, namely, the IEC, three-phase power flow, and conforming and nonconforming currents methods. To do this, computational and experimental simulations are carried out based on employing a system comprised of a perfectly transposed transmission line and two loads that are not always balanced. The cases selected for this study also permit the identification of the main advantages and disadvantages of each method under evaluation. This work is characterized as a topic of great interest to the regulatory agencies responsible for establishing regulations, and for utilities and consumers. It is relevant because by using the methods investigated in this paper, the identification of the sources, which generates unbalance, the division of responsibilities, and the application of penalties due to limit transgression will be possible.
Metabolite channeling in the origin of life.

A central question in the origin of life concerns whether primitive metabolites and catalysts interacted randomly in solution, as often envisaged, or whether they were arranged from the start in ordered metabolic complexes. The latter possibility would be consistent with the hypothesis of metabolite channeling in extant cells, which holds that intermediates in many pathways are transferred directly, without diffusion, between pathway enzymes. A model on this basis is proposed in which life originated autotrophically de novo in metabolic complexes organized on FeS2 (pyrite) mineral surfaces. Because metabolites and catalysts arose at specific sites in these complexes, they could interact specifically with neighbouring species in evolving pathways prior to the existence of protein enzymes with precise substrate binding sites. In successive stages, RNA catalysts and protein enzymes could be incorporated in these arrays. The overall process may be viewed as a molecular analogue of embryonic development, with the formation and positioning of each new component continuously transforming the whole. A corollary of the hypothesis relates to the evolution of translation and the genetic code. By virtue of channeling, biosynthetically related amino acids (e.g., aspartic acid and threonine) would have arisen close together in the complex. A second premise is that tRNAs with similar base sequences, and thus similar anticodons, were also clustered together in the complex and channeled to adjacent sites. As a result of these combined effects, tRNAs with similar anticodons would necessarily have been positioned close to, and thus more likely to have been charged with, metabolically related amino acids. This mechanism affords a new rationale for the observed codon structure of the genetic code, in which biosynthetically related amino acids possess similar codons.
Pulmonary Involvement in Mixed Connective Tissue Disease: High-Resolution CT Findings in 41 Patients

The objective of this study was to describe the pulmonary abnormalities on high-resolution computed tomography (CT) in patients with mixed connective tissue disease (MCTD). The study included 41 patients who met the diagnostic criteria for MCTD and showed abnormal findings on high-resolution CT. The presence, extent, and distribution of various high-resolution CT findings were evaluated. The predominant abnormalities included areas of ground-glass attenuation (n = 41), subpleural micronodules (n = 40), and nonseptal linear opacities (n = 32). Other common findings included peripheral predominance (n = 40), lower lobe predominance (n = 39), intralobular reticular opacities (n = 25), architectural distortion (n = 20), and traction bronchiectasis (n = 18). Less common findings included honeycombing, ill-defined centrilobular nodules, airspace consolidation, interlobular septal thickening, thickening of bronchovascular bundles, bronchial wall thickening, bronchiectasis, and emphysema. Pulmonary involvement of MCTD is characterized by the presence of ground-glass attenuation, nonseptal linear opacities, and peripheral and lower lobe predominance. Ill-defined centrilobular opacities were uncommonly seen.
Arsenic Sensing and Resistance System in the Cyanobacterium Synechocystis sp. Strain PCC 6803

ABSTRACT Arsenic is one of the most important global environmental pollutants. Here we show that the cyanobacterium Synechocystis sp. strain PCC 6803 contains an arsenic and antimony resistance operon consisting of three genes: arsB, encoding a putative arsenite and antimonite carrier, arsH, encoding a protein of unknown function, and arsC, encoding a putative arsenate reductase. While arsB mutant strains were sensitive to arsenite, arsenate, and antimonite, arsC mutants were sensitive only to arsenate. The arsH mutant strain showed no obvious phenotype under the conditions tested. In vivo the arsBHC operon was derepressed by oxyanions of arsenic and antimony (oxidation state, +3) and, to a lesser extent, by bismuth (oxidation state, +3) and arsenate (oxidation state, +5). In the absence of these effectors, the operon was repressed by a transcription repressor of the ArsR/SmtB family, encoded by an unlinked gene termed arsR. Thus, arsR null mutants showed constitutive derepression of the arsBHC operon. Expression of the arsR gene was not altered by the presence of arsenic or antimony compounds. Purified recombinant ArsR protein binds to the arsBHC promoter-operator region in the absence of metals and dissociates from the DNA in the presence of Sb(III) or As(III) but not in the presence of As(V), suggesting that trivalent metalloids are the true inducers of the system. DNase I footprinting experiments indicate that ArsR binds to two 17-bp direct repeats, with each one consisting of two inverted repeats, in the region from nucleotides −34 to + 17 of the arsBHC promoter-operator.
Dysglycaemia and its risk factors in an urban Fulani population of northern Nigeria.

BACKGROUND The Fulani are a normally nomadic people known for covering great distances on foot with a resulting lean physique and presumably low incidence of diabetes mellitus (DM). The occurrence of glucose intolerance among the urban Nigerian Fulani has not been reported. OBJECTIVE To determine the prevalence of dysglycaemia and its correlates among urban Fulani. METHODS Three hundred and eighty-nine Fulani were recruited from urban areas of Sokoto state using a multi-stage sampling technique. Using a modification of the WHO STEPS instrument, information on socio-demographic and anthropometric data was gathered. Either casual or fasting plasma glucose was obtained in all subjects while oral glucose tolerance test (OGTT) was performed in a randomly selected subset of 48 subjects. Glucose intolerance or dysglycaemia was defined using WHO criteria as the presence of diabetes mellitus, impaired fasting glycaemia (IFG) or impaired glucose tolerance (IGT). Classifications of adiposity as body mass index (BMI) and waist circumference (WC) and blood pressure were made using standard criteria. RESULTS Of the 389 subjects studied, 190 (48.8%) were females while 199 (51.2%) subjects were males (c2=5.43, p> 0.05). The overall mean (SD) age of the sample population was 39.3 (14.2) years with the males [42.0 (13.8) years] being significantly older than the females [36.4 (14.1) years], p< 0.05. Eighteen (4.6%) subjects had previously undiagnosed type 2 diabetes mellitus while 37(16.9%) of the subjects had IFG and seven (14.6%) subjects had impaired glucose tolerance. The mean FPG was higher in the males [5.49 (1.7) mmol/l] than in the females [5.25 (1.9) mmol/l],p=0.33. The overall mean two-hour plasma post glucose-load was 6.5 (1.6) mmol/l; higher in the males [6.9 (2)] mmol/l than in the females [6.2 (1) mmol/l] , p=0.55. The mean age, BMI, waist circumference and blood pressure of subjects with dysglycaemia were significantly higher than those in subjects with normoglycaemia (p<0.05). CONCLUSION Prevalence of dysglycaemia in the urban Fulani population of Northern Nigeria is high and much higher in the male than female Fulani. Occurrence of obesity and hypertension appears lower than in other Nigerian reports. Occurrence of dysglycaemia in the urban Fulani increases with age, weight and waist circumference. There is need to conduct a similar study among rural Fulani to better appreciate the environmental influence on these variables.
Perceptions of literature: a comparison of students' and educators' views

This paper reports on an investigation into the perceptions of literature among students of Italian language at the Australian National University. While debate on the role of literature in second-language curricula has been under way for a long time, there has been little attention to students’ perspectives on this question. There is a need for qualitative studies, focused on learners, to explore the relationship between literature, language and students. The theoretical framework for the study presented in this paper is based on some key concepts of phenomenography, especially the role of awareness (Marton & Booth 1997) as a fundamental factor in bringing about change in students’ learning outcomes. The aims of the study, conducted in 1998 and 1999, were: first, to gather students’ perceptions of literature; second, to compare them with educators’ views; and third, to work towards a pedagogy of “awareness and change”, as defined by Marton and Booth. In the conclusion to the paper I propose a view of literature in the L2 context that integrates both students’ and educators’ perspectives, and outline implications for classroom practice.
Carboxymethyl xanthan microparticles as a carrier for protein delivery

Xanthan gum (XG) was derivatized to sodium carboxymethyl xanthan gum (SCMXG) and then cross-linked with aluminium ions (Al+3) to prepare BSA-loaded microparticles (MPs) from a completely aqueous environment. The derivatized gum was characterized by various physical methods. Discrete and spherical BSA-loaded MPs were obtained from SCMXG solution, the pH of which was adjusted to 6 and 7 and the BSA entrapment efficiency was found to reach as high as 82%. The protein release in acidic dissolution medium was faster than that in alkaline dissolution medium and was accounted for the higher swelling ratio of the MPs in acidic environment. Moreover, the pH of the gum solution used to prepare the MPs also influenced the swelling and consequently protein release considerably.