Miracle-dz/UniFAR-FTUs huggingface dataset:
Occult bacteremia from a pediatric emergency department: current prevalence, time to detection, and outcome.

OBJECTIVE To evaluate selected characteristics of occult bacteremia in the post-Haemophilus influenzae type b (HIB) vaccine era. METHODS A retrospective cohort study was performed involving 5901 children 2 to 24 months old with fever >/=39.0 degrees C evaluated with a blood culture at an urban tertiary care children's hospital emergency department (ED) between February 1993 and June 1996. Patients were excluded if immune-suppressed, diagnosed with a focal infection, evaluated by lumbar puncture, or admitted to the hospital during initial evaluation. Prevalence of occult bacteremia, distribution of current pathogenic organisms, and time to positive culture in a continuously monitored system were determined. All patients with cultures positive for pathogenic bacteria were reevaluated and serious adverse outcomes were documented. RESULTS The prevalence of occult bacteremia was 1.9% (95% confidence interval: 1.5%-2.3%). Streptococcus pneumoniae accounted for 82.9% of all pathogens and H influenzae was not a causative organism in this cohort. The mean time to positive culture was significantly shorter for pathogens compared with contaminants (14.9 hours vs 31.1 hours). A culture that was positive in </=18 hours was 13.0 (6.3-26. 6) times more likely to contain a pathogen than a contaminant. The average time from positive culture notification to reevaluation in the ED was 10.6 hours and over half of the patients recalled to the ED for positive cultures were admitted to the hospital. Of patients with occult pneumococcal bacteremia, 95.7% had resolution of their bacteremia without the use of parenteral antibiotics. Two patients had serious adverse outcomes. The rate of meningitis or death was. 03% (.004%-.12%). The contamination rate of blood cultures was 2.1% (1.7%-2.5%). Most (85%) of these patients were reevaluated in the ED and more than one third were admitted to the hospital before full identification of the organism. CONCLUSIONS Prevalence of occult bacteremia in the post-HIB vaccine era is lower than previously reported. S pneumoniae is the most common causative organism and resolves without parenteral antibiotics in the vast majority of cases. Continuously monitoring blood culture systems allow for early identification and can aid in differentiating contaminated from true pathogenic cultures by time to positive culture. Serious adverse outcome is an uncommon result of occult bacteremia. Updated epidemiology and microbiologic technology may impact the evaluation and treatment of children at risk for occult bacteremia.
Comparison between Closing-Wedge and Opening-Wedge High Tibial Osteotomy in Patients with Medial Knee Osteoarthritis: A Systematic Review and Meta-analysis

Abstract Young active patients with medial knee osteoarthritis (OA) combined with varus leg alignment can be treated with high tibial osteotomy (HTO) to stop the progression of OA and avoid or postpone total knee arthroplasty (TKA). Closing-wedge osteotomy (CWO) and opening-wedge osteotomy (OWO) are the most commonly used osteotomy techniques. The purpose of this study was to compare the clinical and radiologic outcomes and complications between OWO and CWO. We retrospectively evaluated 23 studies including 17 clinical trials from published databases from their inception to May 2015. We evaluated the clinical outcomes including operation time, visual analog scale (VAS), maximal flexion, and hospital for special surgery knee (HSS) score. The radiologic outcomes included patellar height measured by posterior tibial slope angle, hip-knee-ankle (HKA) angle, femorotibial (FT) axis, and limb length. Complications recorded included the incidence of deep vein thrombosis (DVT), common peroneal nerve injury, opposite cortical fracture, etc. There were no differences in most of the clinical outcomes except the operation time. OWO increased the posterior slope angle and limb length, decreased the patellar height, and provided higher accuracy of correction. CWO led to a higher incidence of opposite cortical fracture.
Exaggerated block of hERG (KCNH2) and prolongation of action potential duration by erythromycin at temperatures between 37 degrees C and 42 degrees C.

BACKGROUND Environmental and genetic factors interact to define susceptibility to drug-induced long QT syndrome. Although erythromycin induces long QT syndrome, substantial variability exists with regard to its incidence. OBJECTIVES Because fever frequently results in empiric antibiotic usage, we assessed whether temperatures over the range from 36 degrees to 42 degrees C determined responsiveness to erythromycin (100 micromol/L). METHODS I(hERG) was recorded in mammalian cells, and action potentials were recorded in neonatal ventricular mouse myocytes. RESULTS Erythromycin (100 micromol/L) produced no block of I(hERG) at 22 degrees C but produced significant block at 37 degrees C. Extent of block of I(hERG) increased linearly (r = 0.46, P < .01) as temperature increased between 36 degrees C and 42 degrees C. To assess physiologic relevance, action potential duration (APD) was recorded at temperatures between 36 degrees C and 42 degrees C in neonatal ventricular myocytes. Significantly greater prolongation of APD by erythromycin was observed at 42 degrees C compared with 37 degrees C. To assess whether transmembrane diffusion of erythromycin was the rate-limiting step for block of I(hERG) at 22 degrees C, erythromycin was applied within the patch pipette. Under these conditions, erythromycin rapidly blocked I(hERG) even at 22 degrees C. The F656C mutation in the distal S6 of KCNH2 completely abrogated block of I(hERG) measured at 37 degrees C. CONCLUSION Progressively greater block of hERG and prolongation of APD by erythromycin was observed at temperatures between 36 and 42 degrees C. Temperature-dependent block of I(hERG) is explained by temperature-dependent access of erythromycin to the intracellular binding site at F656.
Comparative efficacy of standard antihookworm drugs against various test nematodes

ABSTRACT The chemotherapeutic responses of three test nematodes, Nippostrongylus brasilietxsis, Nematospiroidesdubius and Ancylostoma ceylanicum to standard antihookworm drugs were assessed in order to select asuitable host-parasite system for the primary screening of potential antihookworm compounds. N. dubiusbehaved inconsistently and, with some infections, required more drug to achieve 100% clearance. Nippostrongylus brasiliensis was found to be sensitive to thiabendazole, tetramisole and levamisole but the broad spectrum anthclmintic mebendazole was ineffective. A. ceylanicum was very sensitive to mebendazole, sensitive to tetramisole and levamisole and refractory to thiabendazole. In vitro, none of the compounds had any lethal effect against any of the nematodes, except mebendazole against A. ceylanicum. A. ceylanicum does occur in man and its chemotherapeutic reactions are similar to those of target hookworm infections of economic importance. As such, although not equally sensitive to standard anthelmintics, it is recommended for routine primary screening.
Structure of AscE and induced burial regions in AscE and AscG upon formation of the chaperone needle‐subunit complex of type III secretion system in Aeromonas hydrophila

In the type III secretion system (T3SS) of Aeromonas hydrophila, the putative needle complex subunit AscF requires both putative chaperones AscE and AscG for formation of a ternary complex to avoid premature assembly. Here we report the crystal structure of AscE at 2.7 Å resolution and the mapping of buried regions of AscE, AscG, and AscF in the AscEG and AscEFG complexes using limited protease digestion. The dimeric AscE is comprised of two helix–turn–helix monomers packed in an antiparallel fashion. The N‐terminal 13 residues of AscE are buried only upon binding with AscG, but this region is found to be nonessential for the interaction. AscE functions as a monomer and can be coexpressed with AscG or with both AscG and AscF to form soluble complexes. The AscE binding region of AscG in the AscEG complex is identified to be within the N‐terminal 61 residues of AscG. The exposed C‐terminal substrate‐binding region of AscG in the AscEG complex is induced to be buried only upon binding to AscF. However, the N‐terminal 52 residues of AscF remain exposed even in the ternary AscEFG complex. On the other hand, the 35‐residue C‐terminal region of AscF in the complex is resistant to protease digestion in the AscEFG complex. Site‐directed mutagenesis showed that two C‐terminal hydrophobic residues, Ile83 and Leu84, of AscF are essential for chaperone binding.
Mechanical valve replacement in congenital heart disease.

BACKGROUND AND AIMS OF THE STUDY Mechanical valves are the prosthesis of choice in valve replacement in children. However, the problem of somatic growth leading to patient-valve mismatch remains present, and the appropriate anticoagulation regimen remains controversial. We present our experience of valve replacement in a young population over 20 years. MATERIALS AND METHODS Between 1972 and 1992, 48 patients (34 males and 14 females), mean age 11.2 years (range 0.4-27.4 years), underwent mechanical valve replacement at our institution. Aortic valve replacement was performed in 28 patients (58.3%), mitral valve replacement in 13 (27.1%), tricuspid valve replacement in six (12.5%) and pulmonary valve replacement in one patient (2.1%). The prostheses used were: St. Jude Medical (n = 2), Björk-Shiley (n = 14), Medtronic Hall (n = 16), Duromedics (n = 2) and CarboMedics (n = 14). Early mortality was 14.3%, 10.7% for aortic valve replacement and 30.8% for mitral valve replacement. Mean follow up for all patients was 8.3 years (range 0-22 years), with a total of 398 patient-years. RESULTS Seven patients died during the follow up (17.1%). Survival after 10 years, including operative mortality, was 81% for aortic valve replacement, 33% for mitral valve replacement, 83% for tricuspid valve replacement and 100% for pulmonary valve replacement. All patients were anticoagulated with warfarin. In eight patients (16.7%) an antiplatelet drug (aspirin or dipyridamole) was added. Major events included paravalvular leak in six patients (1.5%/pty), valve thrombosis in five (mitral position in two, tricuspid in three) (1.3%/pty) and endocarditis in one patient (0.3%/pty). Minor thromboembolic events occurred in three patients (0.8%/pty) and minor hemorrhagic events in three (0.8%/pty). No patients developed hemolytic anemia and there was no case of structural failure. CONCLUSIONS In our experience, mechanical prostheses in congenital heart disease were associated with significant morbidity and mortality, however long term survival after aortic valve replacement was good (81% at 10 years). Thromboembolic and hemorrhagic events were of minor significance. Atrio-ventricular valve replacement carried the highest risk of valve thrombosis and we now give warfarin and an antiplatelet drug to children undergoing mechanical valve implantation in this position.
Reactive oxygen species and UV-B: effect on cyanobacteria

Reactive oxygen species (ROS) are involved in the damage and response of cyanobacteria to UV-B irradiation. In cyanobacteria, there are several targets for the potentially toxic ROS such as lipids, DNA and protein. The damage to photosynthetic apparatus induces the inhibition of photosynthesis that is mediated partially by ROS. UV-B-induced oxidative stress and oxidative damage increases with irradiation time and can be reversed after long-term irradiation. This raises the interesting question of whether cyanobacteria can acclimatize to the present UV-B stress. On one hand, ROS may also act as signal molecules and mediate the genetic regulation of photosynthetic genes and the induction of antioxidant enzymes. On the other hand, the efficient defense and repair system allows cyanobacteria to recover from the oxidative damage under moderate UV-B irradiation. In addition, the following methods are discussed: the fluorogenic probe 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), used to detect oxidative stress induced by UV-B; thiobarbituric acid reactive substances (TBARS), used to determine lipid peroxidation in cyanobacteria; fluorimetric analysis of DNA unwinding (FADU), used to quantify DNA strand breaks induced by ROS formation under UV-B stress.
Organization of dimethyl sulfoxide reductase in the plasma membrane of Escherichia coli

Dimethyl sulfoxide reductase is a trimeric, membrane-bound, iron-sulfur molybdoenzyme induced in Escherichia coli under anaerobic growth conditions. The enzyme catalyzes the reduction of dimethyl sulfoxide, trimethylamine N-oxide, and a variety of S- and N-oxide compounds. The topology of dimethyl sulfoxide reductase subunits was probed by a combination of techniques. Immunoblot analysis of the periplasmic proteins from the osmotic shock and chloroform wash fluids indicated that the subunits were not free in the periplasm. The reductase was susceptible to proteases in everted membrane vesicles, but the enzyme in outer membrane-permeabilized cells became protease sensitive only after detergent solubilization of the E. coli plasma membrane. Lactoperoxidase catalyzed the iodination of each of the three subunits in an everted membrane vesicle preparation. Antibodies to dimethyl sulfoxide reductase and fumarate reductase specifically agglutinated the everted membrane vesicles. No TnphoA fusions could be found in the dmsA or -B genes, indicating that these subunits were not translocated to the periplasm. Immunogold electron microscopy of everted membrane vesicles and thin sections by using antibodies to the DmsABC, DmsA, DmsB subunits resulted in specific labeling of the cytoplasmic surface of the inner membrane. These results show that the DmsA (catalytic subunit) and DmsB (electron transfer subunit) are membrane-extrinsic subunits facing the cytoplasmic side of the plasma membrane.
Bidirectional alterations of hippocampal cannabinoid 1 receptors and their endogenous ligands in a rat model of alcohol withdrawal and dependence.

BACKGROUND The hippocampus is strongly implicated in memory processes and contains high concentrations of both cannabinoid receptors and their endogenous ligands. Chronic alcohol consumption impairs a variety of cognitive and performance tasks, including memory and learning. As the activation of cannabinoid receptors by their endogenous ligands modulates hippocampal neurotransmission, we hypothesized that the impaired memory and learning in alcoholism may be due to alterations in the hippocampal endocannabinoid system. METHODS We used the rat chronic intermittent ethanol (CIE) model for alcohol withdrawal and dependence which involves intermittent episodes of ethanol intoxication (60 doses) and withdrawal (approximating binge drinking episodes in humans). We measured the levels of cannabinoid 1 receptor (CB1R) protein (Western blot using a C-terminal-directed antibody), CB1R mRNA (real-time RT-PCR), CB1R localization (immunocytochemistry), tissue levels of the endocannabinoids N-arachidonoylethanolamine/anandamide (AEA) and 2-arachidonoylglycerol (2-AG), and function (patch-clamp recordings of depolarization-induced suppression of inhibition (DSI), as well as effects of CB1R agonist WIN 55,212-2 on inhibitory currents) in the hippocampus of CIE rats and their saline-treated controls. RESULTS Results were obtained in saline and CIE-treated rats after 2 and 40 days of withdrawal (DW) from their respective treatments. In 2 DW CIE rats, CB1R mRNA and protein levels were decreased by 27% (p<0.05) compared with saline controls. Surprisingly, in 40 DW CIE rats, CB1R mRNA increased by 100% and protein increased by 21%, confirmed by immunohistochemistry. Hippocampal [2-AG] increased in both 2 and 40 DW CIE rats; [AEA] increased only at 40 DW. Hippocampal DSI of CIE rats was significantly reduced at 2 DW but not at 40 DW. The CB1R agonist WIN 55,212-2 (0.5 microM) produced a significantly greater decrease in the frequency of spontaneous inhibitory currents from saline-treated rats compared with CIE rats at 2 DW, but not at 40 DW. CONCLUSIONS These data demonstrate that CIE treatment and withdrawal transiently down-regulates hippocampal CB1 Rs followed by a long-term up-regulation, including increased levels of endogenous cannabinoids. These findings are consistent with our hypothesis and suggest that long-term up-regulation of hippocampal CB1Rs may contribute to the long-term cognitive impairments in alcoholism. The data further suggest that the effectiveness of CB1R blockade in decreasing alcohol consumption may be greater after protracted abstinence from alcohol.
Aging of Male Germ Line Stem Cells in Mice1

Abstract In the present study, we investigated the effect of aging on spermatogonial stem cells (SSCs) and on the testicular somatic environment in ROSA26 mice. First, we examined testis weights at 2 mo, 6 mo, 1 yr, and 2 yr of age. At 1 and 2 yr, bilateral atrophied testes were observed in 50% and 75% of the mice, respectively; the rest of the mice had testis weights similar to those of young mice. Next, we evaluated the number and the activity of aged SSCs using spermatogonial transplantation. Numbers of SSCs in atrophied testes decreased in an age-dependent manner to as low as 1/60 of those in testes of young mice. Numbers of SSCs in nonregressed testes were similar regardless of age. The colony length, which is indicative of the potential of SSCs to regenerate spermatogenesis, was similar with donor cells from atrophied testes of 1-yr-old mice and those from testes of young mice, suggesting that SSCs remaining in 1-yr atrophied testes were functionally intact. Colonies arising from SSCs derived from 2-yr atrophied testes were significantly shorter, however, indicating that both SSC numbers and activity declined with age. Finally, we transplanted donor cells from young animals into 1- and 2-yr atrophied testes. Although the weight of 2-yr testes did not change after transplantation, that of 1-yr testes increased significantly, indicating that 1-yr, but not 2-yr, atrophied testes are permissive for regeneration of spermatogenesis by SSCs from young mouse testes. These results demonstrate that both SSCs and somatic environment in the testis are involved in the aging process.
A Novel Feature of the EEG based Motor Imagery BCI System: Degree of Imagery

—Motor imagery recognition has been considered an important topic in the brain-computer interface (BCI) community. Due to noises and artifacts in signals, how to gain satisfactory classification accuracy is still a critical issue. We propose in this paper a novel feature to address this issue. The method consists of three steps. Firstly, EEG signals from different electrodes are transformed by Time-Frequency Analysis method, in this paper Hilbert-Huang Transform. A set of features, Degree of Imagery (DOI) are then extracted from the spectrums by the proposed feature extraction method. The features can effectively represent the event-related-desynchronization (ERD) during motor imagery. Experiments on the BCI 2003 competition dataset III indicate that our method achieves better classification accuracy and higher mutual information (MI) than other researches using the same dataset and with low computational time, which is capable of real-time usage.
Effects of different doses of L-659'066 on the bispectral index and clinical sedation in dogs treated with dexmedetomidine.

OBJECTIVE To evaluate the effects of three doses of L-659'066 (MK-467) on the bispectral index (BIS) and clinical sedation in dexmedetomidine-sedated Beagles. STUDY DESIGN Randomized, experimental cross over study. ANIMALS Eight purpose-bred healthy laboratory Beagles. METHODS Dexmedetomidine (10 μg kg(-1) IV [DEX]) was administered alone or in combination with three doses of L-659'066 (250 μg kg(-1) [DL250]; 500 μg kg(-1) [DL500] and 750 μg kg(-1) [DL750] IV) in the same syringe in a randomized crossover manner. The bispectral index (BIS), electromyography (EMG) and sedation score were recorded at baseline and 5, 10, 20, 30, 45 and 60 minutes after treatment. RESULTS When compared to DEX, BIS and EMG were significantly higher and the sedation score significantly lower with DL500 and DL750. With DEX, BIS was significantly decreased at times 20, 30 and 60 minutes whereas the sedation scores were significantly increased at all time points after drug administration in all groups. Bioequivalence for clinical sedation was detected between DEX and all doses of L-659'066, reaching European Medicines Agency (EMA) standards. CONCLUSIONS AND CLINICAL RELEVANCE Although L-659'066 interfered with dexmedetomidine induced sedation, the degree of the reduction was not clinically relevant. Despite performing better when dexmedetomidine was used alone, BIS did not reflect the clinical sedative status when the antagonist was added.
Increased platelet CD36 constitutes a common marker in myeloproliferative disorders

Summary. The distribution of the major platelet membrane glycoproteins (GP), Ib, IX, IIb‐IIIa and IV (or CD36), which play important roles as receptors for adhesive molecules in haemostasis and thrombosis, was studied in 34 patients with myeloproliferative disorders (MPD): 13 had essential thrombocythaemia (ET), 12 had polycythaemia vera (PV) and nine had chronic myelogenous leukaemia (CML). Only occasionally were modifications of the numbers of GPIb or GPIIb‐IIIa measured using the binding of specific radiolabeled antibodies to platelets. In contrast, 2‐3‐fold increases of the total CD36 content and the surface CD36 expression were measured in almost all patients studied, using a radioimmunoassay and the direct binding of the radiolabelled antibody, FA6‐152, to the platelet surface, respectively. These results indicate that the abnormality affected both the external and internal CD36 pools. Therefore platelet CD36 may be a useful tool for the diagnosis and the follow‐up of MPD patients.
Effect of adhesive agent and fissure morphology on the in vitro microleakage and penetrability of pit and fissure sealants.

OBJECTIVE To evaluate the effect of adhesive agents and fissure morphology on the microleakage and penetrability of pit and fissure sealants. METHOD AND MATERIALS Sealants used in this study included Aegis (Bosworth), conseal f (Southern Dental Industries), and Admira Seal (Voco). Adhesive agents included Optibond Solo Plus (sds/Kerr) and Clearfil S3Bond (Kuraray). Ninety extracted permanent molars were randomly assigned to 9 groups combining sealant and adhesive agent materials. A sealant and control group (phosphoric acid etch only) was also included. Dye penetration (microleakage), penetrability, and fissure morphology assessment was performed for the treatment groups through microscopic evaluation. RESULTS Significant (P = .003) differences in microleakage were exhibited, with the Aegis + control and Aegis + Optibond Solo Plus groups displaying less leakage, while significant (P = .03) differences were also noted between groups regarding penetrability. Fissure morphology was not a significant (P = .82) factor affecting microleakage; however, fissure type did significantly (P < .001) impact penetrability. No correlation was found between the extent of microleakage and penetrability. CONCLUSION Application of sealants using phosphoric acid as a conditioning agent revealed superior results, while the use of adhesives was found to be unnecessary.