Miracle-dz/UniFAR-FTUs huggingface dataset:
Biochemical and Evolutionary Significance of Phospholipid Methylation*

All nucleated mammalian cells synthesize phosphatidylcholine from choline via the CDP-choline pathway. Hepatocytes have a second pathway for the synthesis of phosphatidylcholine, a stepwise methylation of phosphatidylethanolamine, catalyzed by phosphatidylethanolamineN-methyltransferase and encoded by the Pemptgene. We report that when Pempt-deficient mice were fed a choline-deficient diet for 3 days, severe liver pathology occurred apparently due to a lack of phosphatidylcholine biosynthesis. The hepatic concentration of phosphatidylcholine decreased by 50% compared with wild type mice on the diet. The levels of plasma triacylglycerols and cholesterol were decreased by greater than 90% in thePempt-deficient mice. We suggest that the Pemptgene has been maintained during evolution to provide phosphatidylcholine when dietary choline is insufficient, as might occur during starvation or pregnancy.
Transfusion rate associated with hysterectomy for benign disease

The transfusion rate associated with hysterectomy for benign disease is an indirect indicator of haemorrhage. It is used in quality assurance activities and is one measure of standard of care. This retrospective study was conducted to determine the transfusion rate for these operations in a tertiary referral hospital. In addition, it was considered that the information could be used in deciding the need for a routine preoperative group and save policy (G and S). The Blood Bank records of all women undergoing hysterectomy for benign disease from 1993–1998 were examined and the number of women transfused was recorded. A total of 1220 hysterectomies were performed. Of women having vaginal hysterectomies only 0.38% required transfusion compared with 2.18% for abdominal hysterectomies. These data suggest that there is no need for a strict policy of preoperative G and S for all patients. In addition, this information can be used as a benchmark when reviewing morbidity associated with hysterectomy and in particular when various methods of hysterectomy are compared.
Strain-Dependent Augmentation of Tight-Junction Barrier Function in Human Primary Epidermal Keratinocytes by Lactobacillus and Bifidobacterium Lysates

ABSTRACT In this study, we investigated whether probiotic lysates can modify the tight-junction function of human primary keratinocytes. The keratinocytes were grown on cell culture inserts and treated with lysates from Bifidobacterium longum, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus fermentum, or Lactobacillus rhamnosus GG. With the exception of L. fermentum (which decreased cell viability), all strains markedly enhanced tight-junction barrier function within 24 h, as assessed by measurements of transepithelial electrical resistance (TEER). However, B. longum and L. rhamnosus GG were the most efficacious, producing dose-dependent increases in resistance that were maintained for 4 days. These increases in TEER correlated with elevated expression of tight-junction protein components. Neutralization of Toll-like receptor 2 abolished both the increase in TEER and expression of tight-junction proteins induced by B. longum, but not L. rhamnosus GG. These data suggest that some bacterial strains increase tight-junction function via modulation of protein components but the different pathways involved may vary depending on the bacterial strain.
Reconstruction of nasal defects 1.5 cm or smaller.

OBJECTIVES To review the repair of smaller nasal defects (≤1.5 cm) and their associated complications, elaborating on nasal obstruction and techniques to avoid it, and to determine outcomes with functional cartilage grafting. METHODS The medical records of patients requiring nasal reconstruction for Mohs defects 1.5 cm or smaller were reviewed. Variables analyzed included defect location, defect size, use of cartilage, flap design, smoking status, and postoperative complications (nasal obstruction and flap/graft necrosis). RESULTS A total of 208 patients with 213 nasal defects 1.5 cm or smaller were identified. The most common location was the alar subunit, followed by the tip, dorsum, and sidewall. Ninety-eight of the defects (46.0%) used cartilage grafts for reconstruction. Seventy-three of 84 alar defects (86.9%) were reconstructed with cartilage as a composite or batten graft. The sidewall and dorsum were the least likely to require cartilage grafting: 1 of 15 (6.7%) and 0 of 21, respectively. Ten patients (4.8%) had a postoperative complication: 6 of 19 smokers (31.6%) and 4 of 189 nonsmokers (2.1%). Overall, nasal obstruction was an infrequent complication (1.4%). CONCLUSIONS Regardless of defect size, defect location, and flap design, smokers were at higher risk for postoperative complications. Subtle modifications in the classic flap design and liberal use of cartilage grafting reduce the risk of postoperative nasal obstruction.
Knowledge, attitudes, and practices toward malaria risk and prevention among frequent business travelers of a major oil and gas company.

BACKGROUND Despite significant morbidity and mortality among business travelers due to malaria, very little has been published on knowledge, attitudes, and practices (KAP) toward malaria risk. The aim of this study was to assess KAP among frequent international business travelers (FBT) and to identify recommendations for improving malaria prevention that could be applied to the wider FBT community in occupational health. METHODS A retrospective web-based survey was conducted in 2005 among self-registered FBT of an oil and gas company based in the Netherlands. RESULTS The survey was completed by 328 of the 608 self-registered FBT (54%). Fifty-four percent of respondents had visited a high-risk area for malaria. Most respondents (96%) were experienced travelers; the majority (71%) sought health advice before their trip and made use of a company health resource. Fever was recognized as a malaria symptom by all FBT; travel to high-risk malaria areas was correctly identified by 96%, and 99% of these travelers adhered to use of adequate personal protective measures. The proportion of travelers carrying appropriate anti-malaria drug regimen was positively associated with receiving company advice among FBT traveling to high-risk destinations (RR = 2.10, 95% CI: 1.21-3.67), but not for those traveling to low- or no-risk destinations. Only 8% (14) of those going to a high-risk area were not carrying malaria prophylaxis. One in five of FBT traveling to no-risk areas were unnecessarily carrying malaria prophylaxis. CONCLUSIONS The majority of KAP results were excellent. We postulate that a company culture with a strong focus on health, safety, security, and environment can positively contribute to high KAP scores. Notwithstanding the excellent findings, this study also provides a cautionary tale for company health functions against overprescribing of malaria prophylaxis. It demonstrates the need for constant review and audit of adherence to quality criteria.
Clickstream Data as a Source to Uncover con-Sumer Shopping Types in a Large-Scale Online Setting

Today’s technological advancements enable marketers to track consumer touch points in great detail. We analyze the users’ sequence of visits to a website (off-site clickstreams) as it gives insights into the overall decision-making process of consumers on their path to purchase or non-purchase. We show that search patterns based on the online advertising channel choice discloses specific shopping types. Operationalizing search behavior as navigational or informational based on information retrieval research and the level of consumer involvement; we use k-means clustering to categorize search patterns as Buying, Searching, Browsing or Bouncing. Our typology is based on a unique and comprehensive dataset from a leading European fashion e-commerce company and includes a total of almost 30 million clickstream journeys based on over 80 million lines of clicks from 11 advertising channels. This paper is the first to link the off-site online channel journey of consumers with their underlying search patterns to establish a typology of search types in a large-scale setting. Understanding the nature and characteristics of online consumer search and its implications for consideration and choice is also of high importance from a managerial perspective as our results offer e-marketers the ability to make inferences for automated marketing system decision-making.
Corticosteroid‐free immunosuppression with tacrolimus following induction with daclizumab: A large randomized clinical study

This open, randomized (1 : 1), multicenter, 3‐month study compared a dual tacrolimus plus steroids (Tac / steroids) regimen with a steroid‐free immunosuppressive regimen of tacrolimus following daclizumab induction therapy (Tac / Dac) in adult liver transplant recipients. The full analysis set comprised 347 patients in the Tac / steroids group and 351 in the Tac / Dac group. Mean tacrolimus dose during month 3 was 0.11 mg/kg/day in both groups; mean whole‐blood trough levels during month 3 were 10.9 ng/mL (Tac / steroids) and 10.6 ng/mL (Tac / Dac). The incidence of biopsy‐confirmed acute rejection that required treatment was similar in both groups: 26.5% in the Tac / steroids group and 25.4% in the Tac / Dac group (P = .727). However, the incidence of biopsy‐confirmed corticosteroid‐resistant acute rejection was higher in the Tac / steroids group than in the Tac / Dac group (6.3 vs. 2.8%; P = .027). Kaplan‐Meier estimates of graft survival (92.2 vs. 90.5%) and patient survival (94.5 vs. 93.7%) were similar in both groups. While also the overall adverse event profiles were similar, the incidences of diabetes mellitus (15.3 vs. 5.7%, respectively; P < .001) and cytomegalovirus infection (11.5 vs. 5.1%, respectively; P = .002) were higher in the Tac / steroids group compared with the Tac / Dac group. Mean cholesterol levels increased by 16% in the Tac / steroids group, but were unchanged in the Tac / Dac group during the study. In conclusion, tacrolimus monotherapy following daclizumab induction is an effective and safe regimen, with an advantage over concomitant steroid‐maintenance therapy in terms of a lower incidence of diabetes and viral infection, and a lower incidence of steroid‐resistant acute rejection. (Liver Transpl 2005;11:61–67.)
Social Synthesis: A psycho-social perspective of the construction project team

This paper presents a framework resulting from systematic investigation within the field of social psychology, from which to derive new narratives, concepts, and relationships for collaborative design in architecture, engineering, and construction (AEC). A systematic literature review generated a series of themes that had potential for relevance to interdisciplinary built environment project teams. These were then explored, drawing on qualitative research conducted using focus groups drawn from three AEC organisations and observation of a live case-study industry project. The social psychology anchor themes of (1) motivation and reward; (2) risk attitudes; and (3) social climate were then recontextualised using the qualitative data, to derive construction-specific social and psychological factors that influence the collaborative design process. The resultant psycho-social framework applies psychology theory to describe a multiplicity in the role agency of project team members, as actors in industry, discipline, company, and individual contexts. Role agency and domain-specific themes are combined within the collective to influence normative and adaptive responses within the team interaction space, where collective systems of meaning are synthesised and design outcomes produced.
On negation and focus in spanish and basque

In this paper we analize some asymmetries in the interpretation of negative sentences and argue that they follow from differences in the structural configurations produced by syntactic focus. We first concentrate on Spanish and show that there are three different ways in which Negation and focus can interact semantically, which accounts for the range of interpretations displayed by negative sentences in this language. Under the first construal, Negation takes scope over the whole clause, which is interpreted as focalized (wide focus). Under the second construal, Negation takes scope only over the element in final position, which constitutes the focus of the sentence (bound focus). Finally, in the third reading, the focus in final position is out of the scope of Negation (free focus). We show that in Spanish each of these readings is associated to a different syntactic structure. In particular, we propose that the bound and the free readings are derived by movement of the focalized element to the specifier of a Focus Phrase. In the bound focus reading, the focalized element moves to the specifier of a FocP located between NegP and VP, and the rest of the material is scrambled to the left of this FocP. But we argue that the third reading, the free construal of focus, involves a different derivation and a different syntactic structure. In particular, we propose that this reading is derived in two steps. First, the focalized element moves to the specifier of a FocP higher than NegP and, then, the remnant (containing the negative head)
Feasibility and behavioral effects of an at-home multi-night sleep restriction protocol for adolescents.

BACKGROUND Sleep deprivation is common among adolescents and has been associated with adverse behavioral and educational outcomes. However, it is difficult to draw strong causal conclusions because of a dearth of experimental sleep research. In part, this appears related to methodological challenges when working with this population. This study tested the feasibility and behavioral effects of a multi-night, at-home experimental sleep restriction protocol in a sample of adolescents. METHODS Twenty healthy adolescents aged 13.9-16.9 years were enrolled in a three-week sleep manipulation protocol using a counterbalanced cross-over experimental design. The protocol included a baseline week, followed in random order by a short sleep week (Monday-Friday nights limited to 6.5 hours time in bed) and an extended sleep week (10 hours lights-out time in bed Monday-Friday nights). Sleep was monitored via self-report and objective actigraphy. These were reviewed with participants and parents on the Saturdays at the end of each week, when parents and participants also completed behavior rating questionnaires. RESULTS One participant dropped out of the study, but each of the remaining 19 displayed markedly less sleep in the short sleep condition than the extended sleep condition (average nightly gap approximately 2.5 hours). Data also reflected indirect effects of sleep deprivation that are consistent with an increase in homeostatic sleep drive. Compared to the extended sleep week, parents during the short sleep week reported that the participants displayed significantly greater problems with sleepiness, attention, oppositionality/irritability, behavior regulation, and metacognition. Participant self-report results were similar, though less robust. CONCLUSIONS A multi-night, at-home sleep manipulation protocol for use with adolescents is indeed feasible. This study also provided the first experimental evidence that chronic sleep restriction during adolescence is causally related to a wide range of behavioral deficits.
Treating Crohn's Disease by Inducing T Lymphocyte Apoptosis

Abstract: Apoptosis is one of the most important regulatory mechanisms in immunological homeostasis. Disturbances in the apoptotic pathways lead to autoimmune disease. Crohn's disease is a chronic inflammatory bowel disease of unknown origin, which seems to be mediated by excessive T cell‐mediated immunity. Recently, disturbances in apoptotic pathways of lamina propria T lymphocytes of patients with Crohn's disease have been identified. In the uninflamed, normal intestinal mucosa, lamina propria (LP) T cells are susceptible to activation‐induced cell death, but these cells show a resistance to apoptosis based on several disturbances compared to controls. Recently, intriguing data were published using cytokine‐targeted therapy (anti‐IL12, anti‐IL6 receptor, anti‐TNF). Actually, these medications restored mucosal immunological imbalance by inducing apoptosis of the LP T cells and seemed to be beneficial in models of Crohn's disease. In this review, mechanisms of immunological homeostasis will be discussed. We will also discuss the fascinating new results of cytokine‐targeted therapy in animal models of Crohn's disease and the effects of these drugs in patients with Crohn's disease.
The Effect of Amenorrhea on Calcaneal Bone Density and Total Bone Turnover in Runners

To examine in athletes the effect of long-term amenorrhea on the skeleton, measurements of calcaneal density and whole body retention of 99mTc-imidodiphosphate were made in 42 women who could be allocated to one of 3 groups defined by their level of physical activity and by menstrual status. There was no difference in bone density between eumenorrheic normoactive females and either eumenorrheic or amenorrheic athletes. However, calcaneal density was significantly greater for each group than for previously measured sedentary controls. Total body bone turnover was greater in both eumenorrheic and amenorrheic athletes than in eumenorrheic normoactive women. Sustained, intense physical activity does not significantly increase calcaneal bone density over and above the increase associated with normal levels of activity. This is despite a significant increase in the rate of total body bone mineral turnover.
Parent–Teacher Association, Soup Kitchen, Church, or the Local Civic Club? Life Stage Indicators of Volunteer Domain

Gaps in existing literature hinder our knowledge of how life stage-related identities (e.g., worker, parent, student, etc.) influence individuals’ decisions about whether and how to get involved in community service. Interventions to increase volunteerism throughout the life course require a more nuanced understanding of this relationship. We use multinomial logistic models to analyze how life phase factors relate to involvement in different types of voluntary organizations across the adult life course in the Chicago Community Adult Health Study. Half of the adults did not volunteer. Those who did volunteer were categorized as charitable, youth-oriented, religious, civic, or multidomain volunteers. Age, employment, family structure, demographics, and self-rated health differentially predicted volunteering in specific domains. Findings from this study suggest that recruitment and retention efforts employed by different nonprofit organizations may be more effective if they take into consideration the life phase factors that enhance or detract from likelihood of engagement.
Host community resignation to nightclub tourism

Ibiza is world-famous for its nightclubs, parties (legal or not) and its nightlife. The status of ‘party destination’ Ibiza currently holds would be difficult to explain without if not for the surging boom in the 80s. This is when nightclubs such as Pacha, Playboy, Amnesia and Ku became tourist attractions of worldwide renown. Today, Ibiza is the international leader in the clubber tourism. Minimal research or studies have been carried out in order to verify the attitudes of the host community towards this form of leisure tourism. This is a complete contrast to the numerous studies carried out in other sectors, such as casinos. The aim of this paper is to analyse and segment the attitudes of the residents of Ibiza towards nightlife tourism through a quantitative study based on a large survey. Three clear groups are identified: Supporters (27%), Opposers (29%) and Mild Opposers (44%). Overall, results show, on one hand, a high recognition of the importance of clubs and nightlife on the economy and image of the island but, on the other hand, a resigned opposition to this kind of tourism: residents would prefer to prioritize other tourism activities. Just like the casinos, nightclubs are poorly assessed by residents because of some negative effects. Practical and managerial implications are also discussed as well as the convenience to carry out additional research on how to increase host community resilience to cope with nightclub tourism.
Mutations of the Cationic Trypsinogen Gene in Hereditary and Non-Hereditary Pancreatitis

Background/Aims: Mutations in the cationic trypsinogen gene have been detected in patients with hereditary pancreatitis (HP). This study investigates the prevalence of the R122H, N29I, A16V and –28delTCC mutations in the common, non-hereditary forms of chronic pancreatitis and in a HP family. Methods: DNA was prepared from blood samples of 53 patients with chronic pancreatitis (36 alcoholic, 14 idiopathic and 3 hereditary), 20 alcoholic controls and 20 healthy, ethnically matched controls. The R122H and A16V mutations were identified by the polymerase chain reaction (PCR) and restriction enzyme digestion. A nested-PCR was used to identify the N29I mutation. The –28delTCC deletion and the C133807T polymorphism were sought by direct sequencing. Results: The R122H mutation was detected in 1 patient with alcoholic chronic pancreatitis and all 3 affected members of a HP family. The N29I, A16V and –28delTCC mutations were not detected in any of the study subjects. At the C133807T polymorphism, the C allele and C/C genotype were significantly increased in alcoholic chronic pancreatitis (p = 0.001 and p = 0.0004, respectively) while the T allele and CT genotype were significantly reduced (p = 0.001 and p = 0.004, respectively) compared to healthy controls. Conclusions: Mutations of the cationic trypsinogen gene are rarely found in chronic pancreatitis patients of typical aetiology. Screening for these mutations should be considered in those with a family history consistent with hereditary pancreatitis but may also be appropriate in a well-defined subgroup of patients with non-hereditary chronic pancreatitis, i.e. those who have developed the disease before the age of 30.