Miracle-dz/UniFAR-FTUs huggingface dataset:
Technology substitution and policy design in an ABM model : the case of REACH

From the start, REACH was designed to balance environmental objectives with competitive aims, and has the scope to induce the development and adoption of eco-innovation as a side effect of the regulation itself. In the economic literature, many authors have emphasized a positive correlation between innovation and environmental regulation [1], [2], [3]. However, eco-innovations cannot be considered a systematic response to regulation. Policy design ends up being essential to inducing the development of eco-innovations [4], [5], [6]. In this respect, a number of criteria, such as stringency, flexibility, timing and credibility, are important factors to consider. REACH seems to fit perfectly in this context and appears to be a privileged object of study to analyze how policy design can stimulate ecoinnovations. on industrial dynamics. This regulation introduces a new legislative philosophy about how to handle chemicals, especially by adopting three essential principles: the “principle of reversal of the burden of proof” from authorities to industry by which manufacturers and importers of chemicals must register each substance and assess the health and environmental risks associated to avoid being excluded from the market (“No data, no market”); the extended responsibility to users, which are now closely associated with regulatory compliance, potentially widening the impact of REACH to all the industries; and the authorization and restriction of the most dangerous substances.
Phylogenetic relationships of Eurasian and American cyprinids using cytochrome b sequences

Neighbour-joining and parsimony analyses identified five lineages of cyprinids: (1) European leuciscins (including Notemigonus)+North American phoxinins (including Phoxinus phoxinus); (2) European gobionins+Pseudorasbora; (3) primarily Asian groups [cultrins+acheilognathins+ gobionins (excluding Abbotina)+xenocyprinins]; (4) Abbottina+Sinocyclocheilus+Acrossocheilus; (5) cyprinins [excluding Sinocyclocheilus and Acrossocheilus]+barbins+labeonins. Relationships among these lineages and the enigmatic taxa Rhodeus were not well-resolved. Tests of monophyly of subfamilies and previously proposed relationships were examined by constraining cytochrome b sequences data to fit previous hypotheses. The analysis of constrained trees indicated that sequence data were not consistent with most previously proposed relationships. Inconsistency was largely attributable to Asian taxa, such as Xenocypris and Xenocyprioides. Improved understanding of historical and taxonomic relationships in Cyprinidae will require further morphological and molecular studies on Asian cyprinids and taxa representative of the diversity found in Africa.
Chapter I

1.0 Introduction With more than 50 per cent of the geographical area, and 65 per cent of the people living in rural area, the economic development of the rural areas of the country of India greatly determines the development of the economy 1 . Among them, the women in rural areas constitute a sizeable portion. Since women have a crucial role to play in the modern society, it is felt that empowering women is a sure path to achieve major economic gains. With the concept of inclusive growth assuming prominence in the policy agenda of the government, it was felt pertinent to provide opportunities to women folks to take active part in nation building. However, with poor family income and little scope for employment in rural sector, it was felt pertinent to extend sufficient credit to women folks to contribute to the economy‟s output through creation of self employment. The formation of Self Help Groups (SHGs) facilitates for such a development. Today, SHGs have come out successfully in creating employment to both rural and urban women folks, in increasing the output of micro enterprises and above all, in improving the economic and social status of women of this great country. However, the SHGs face various challenges in process of their development. More specifically, the social, economic and demographic factors hinder their active participation and even their enrollment. The problems faced by them in the process of carrying out their SHG activities almost resembles the problems of any small scale units. In the present project it is attempted to examine the socio economic status of the women SHG entrepreneurs, the various problems faced by the SHGs and the social and economic impact of SHG activities by way of increase in their household income and improvement in their societal status in the context of the selected SHGs in the district of Salem.
Fertility Preservation Using GnRH Agonists: Rationale, Possible Mechanisms, and Explanation of Controversy

The only clinically accepted method of fertility preservation in young women facing gonadotoxic chemo- and/or radiotherapy for malignant or autoimmune diseases is cryopreservation of embryos or unfertilized ova, whereas cryopreservation of ovarian tissue for future reimplantation, or in vitro maturation of follicles, and the use of gonadotropin-releasing hormone agonists (GnRHa) are still considered investigational, by several authorities. Whereas previous publications have raised the fear of GnRHa’s possible detrimental effects in patients with hormone receptor-positive breast cancers, recent randomized controlled trials (RCTs) have shown that it either improves or does not affect disease-free survival (DFS) in such patients. This review summarizes the pros and cons of GnRHa co-treatment for fertility preservation, suggesting 5 theoretical mechanisms for GnRHa action: (1) simulating the prepubertal hypogonadotropic milieu, (2) direct effect on GnRH receptors, (3) decreased ovarian perfusion, (4) upregulation of an ovarian-protecting molecule such as sphingosine-1-phosphate, and (5) protecting a possible germinative stem cell. We try to explain the reasons for the discrepancy between most publications that support the use of GnRHa for fertility preservation and the minority of publications that did not support its efficiency.
The effects of sensory-processing sensitivity and sense of coherence on depressive symptoms in university students

Sensory-processing sensitivity differentiates individuals according to responsivity to internal and external stimuli. It has been positively correlated with depressive symptoms. Meanwhile, sense of coherence, an individual’s perception that stressors are comprehensible, manageable, and meaningful for their life, could improve depression. This cross-sectional study investigated the moderation effect of sense of coherence on the relationship between sensory-processing sensitivity and depressive symptoms in university students. Japanese students (N = 430) participated in a questionnaire survey that assessed levels of sensory-processing sensitivity, sense of coherence, and depressive symptoms. The results showed that a strong sense of coherence moderated the relationship between sensory-processing sensitivity and depressive symptoms in university students.
Maximizing Longevity and Healthspan: Multiple Approaches All Converging on Autophagy

Our understanding of the molecular basis of aging has greatly increased over the past few decades. In this review, we provide an overview of the key signaling pathways associated with aging, and whose modulation has been shown to extend lifespan in a range of model organisms. We also describe how these pathways converge onto autophagy, a catabolic process that functions to recycle dysfunctional cellular material and maintains energy homeostasis. Finally, we consider various approaches of therapeutically modulating these longevity pathways, highlighting exercise as a potent geroprotector.
Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis

Human milk oligosaccharide transporters play key roles in symbiosis and coevolution between bifidobacteria and breast-fed infants. The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that human milk oligosaccharides (HMOs) in breast milk promote the formation of a bifidobacteria-rich microbiota in infant guts; however, the underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from Bifidobacterium longum subspecies infantis. Fecal DNA and HMO consumption analyses, combined with deposited metagenome data mining, revealed that FL transporter-2 is primarily associated with the bifidobacteria-rich microbiota formation in breast-fed infant guts. Structural analyses of the solute-binding protein (SBP) of FL transporter-2 complexed with 2′-fucosyllactose and 3-fucosyllactose, together with phylogenetic analysis of SBP homologs of both FL transporters, highlight a unique adaptation strategy of Bifidobacterium to HMOs, in which the gain-of-function mutations enable FL transporter-2 to efficiently capture major fucosylated HMOs. Our results provide a molecular insight into HMO-mediated symbiosis and coevolution between bifidobacteria and humans.
Karonudib is a promising anticancer therapy in hepatocellular carcinoma

Background: Hepatocellular carcinoma (HCC) is the most common form of liver cancer and is generally caused by viral infections or consumption of mutagens, such as alcohol. While liver transplantation and hepatectomy is curative for some patients, many relapse into disease with few treatment options such as tyrosine kinase inhibitors, for example, sorafenib or lenvatinib. The need for novel systemic treatment approaches is urgent. Methods: MTH1 expression profile was first analyzed in a HCC database and MTH1 mRNA/protein level was determined in resected HCC and paired paracancerous tissues with polymerase chain reaction (PCR) and immunohistochemistry. HCC cancer cell lines were exposed in vitro to MTH1 inhibitors or depleted of MTH1 by siRNA. 8-oxoG was measured by the modified comet assay. The effect of MTH1 inhibition on tumor growth was explored in HCC xenograft in vivo models. Results: MTH1 protein level is elevated in HCC tissue compared with paracancerous liver tissue and indicates poor prognosis. The MTH1 inhibitor Karonudib (TH1579) and siRNA effectively introduce toxic oxidized nucleotides into DNA, 8-oxoG, and kill HCC cell lines in vitro. Furthermore, we demonstrate that HCC growth in a xenograft mouse model in vivo is efficiently suppressed by Karonudib. Conclusion: Altogether, these data suggest HCC relies on MTH1 for survival, which can be targeted and may open up a novel treatment option for HCC in the future.
Baseball Pitching Biomechanics Shortly After Ulnar Collateral Ligament Repair

Background: The probability of returning to competition for injured baseball pitchers is similar after ulnar collateral ligament (UCL) repair as after UCL reconstruction, but the time to return is significantly quicker after UCL repair. Previous research has found no differences in pitching biomechanics between pitchers with and without a history of UCL reconstruction, but pitching biomechanics after UCL repair has not been studied. Hypothesis: There will be significant differences in pitching biomechanics between pitchers returning to play after UCL repair and pitchers with no injury history. Study Design: Controlled laboratory study. Methods: A total of 33 pitchers were tested shortly after UCL repair (9.8 ± 2.6 months) and compared with a matched group of 33 uninjured pitchers. Each group comprised 14 college pitchers and 19 high school pitchers. Shoulder and elbow passive ranges of motion were measured. The biomechanics of 10 fastballs was then collected using a 12-camera automated motion capture system. Ball velocity was measured using a separate 3-camera optical tracking system. Data were compared between the UCL repair group and the control group using the Student t test (significance set at P < .05). Results: There were no differences in passive range of motion or fastball velocity between the 2 groups. There were no differences in joint kinetics during pitching, but 3 kinematic variables showed significant differences. Specifically, the UCL repair group produced less elbow extension (flexion: 27° ± 6° vs 24° ± 4°, respectively; P = .03), less elbow extension velocity (2442 ± 367 vs 2631 ± 292 deg/s, respectively; P = .02), and less shoulder internal rotation velocity (6273 ± 1093 vs 6771 ± 914 deg/s, respectively; P = .049 ) compared with the control group. Conclusion: Elbow extension, elbow velocity, and shoulder velocity differed between pitchers with a recent history of UCL repair and a matched control group, but it is unclear whether this has clinical significance, as there were no differences in ball velocity and passive range of motion. Furthermore, it is unknown whether these few differences in pitching biomechanics resolve with time. Clinical Relevance: Elbow and shoulder kinematics during pitching might not be completely regained within the first year after UCL repair, although passive range of motion and pitch velocity show no difference in comparison to other healthy pitchers.
Introducing a High Performance Mg-Based Multicomponent Alloy as an Alternative to Al-Alloys

In this study, the design and development of a new Mg-based multicomponent alloy (ρ_th= 2.33 g/cc; ρ_ex= 2.15 g/cc) has been presented which exhibits excellent mechanical properties in hot extruded condition. Mg80Al5Cu5Mn5Zn5 alloy was designed and synthesized using disintegrated melt deposition technique followed by hot extrusion. The alloy was designed in such a way that the concentration of each alloying element remained ≥ 5 at % with Mg (80 at %) as base metal. Significantly high hardness (196 HV) was realized in Mg80Al5Cu5Mn5Zn5 alloy. Compression results showed substantially high ultimate compressive strength (UCS) 616 MPa of this alloy with a reasonably high ductility of 16.9%. This alloy also exhibited superior combination of tensile strengths (TYS- 211 MPa, UTS- 318 MPa) and ductility (8.2%). Overall combination of hardness, tensile and compression properties showed the current new light weight alloy system as an alternative to most of the commercially used Al-based alloys.
Global distribution and diversity of Chaetoceros (Bacillariophyta, Mediophyceae): integration of classical and novel strategies

Information on taxa distribution is a prerequisite for many research fields, and biological records are a major source of data contributing to biogeographic studies. The Global Biodiversity Information Facility (GBIF) and the Ocean Biogeographic Information System (OBIS) are important infrastructures facilitating free and open access to classical biological data from several sources in both temporal and spatial scales. Over the last ten years, high throughput sequencing (HTS) metabarcoding data have become available, which constitute a great source of detailed occurrence data. Among the global sampling projects that have contributed to such data are Tara Oceans and the Ocean Sampling Day (OSD). Integration of classical and metabarcoding data may aid a more comprehensive assessment of the geographic range of species, especially of microscopic ones such as protists. Rare, small and cryptic species are often ignored in surveys or mis-assigned with the classical approaches. Here we show how integration of data from various sources can contribute to insight in the biogeography and diversity at the genus- and species-level using Chaetoceros as study system, one of the most diverse and abundant genera among marine planktonic diatoms. Chaetoceros records were extracted from GBIF and OBIS and literature data were collected by means of a Google Scholar search. Chaetoceros references barcodes where mapped against the metabarcode datasets of Tara Oceans (210 sites) and OSD (144 sites). We compared the resolution of different data sources in determining the global distribution of the genus and provided examples, at the species level, of detection of cryptic species, endemism and cosmopolitan or restricted distributions. Our results highlighted at genus level a comparable picture from the different sources but a more complete assessment when data were integrated. Both the importance of the integration but also the challenges related to it were illustrated. Chaetoceros data collected in this study are organised and available in the form of tables and maps, providing a powerful tool and a baseline for further research in e.g., ecology, conservation and evolutionary biology.
Brain–Computer Interface-Based Adaptive Automation to Prevent Out-Of-The-Loop Phenomenon in Air Traffic Controllers Dealing With Highly Automated Systems

Increasing the level of automation in air traffic management is seen as a measure to increase the performance of the service to satisfy the predicted future demand. This is expected to result in new roles for the human operator: he will mainly monitor highly automated systems and seldom intervene. Therefore, air traffic controllers (ATCos) would often work in a supervisory or control mode rather than in a direct operating mode. However, it has been demonstrated how human operators in such a role are affected by human performance issues, known as Out-Of-The-Loop (OOTL) phenomenon, consisting in lack of attention, loss of situational awareness and de-skilling. A countermeasure to this phenomenon has been identified in the adaptive automation (AA), i.e., a system able to allocate the operative tasks to the machine or to the operator depending on their needs. In this context, psychophysiological measures have been highlighted as powerful tool to provide a reliable, unobtrusive and real-time assessment of the ATCo’s mental state to be used as control logic for AA-based systems. In this paper, it is presented the so-called “Vigilance and Attention Controller”, a system based on electroencephalography (EEG) and eye-tracking (ET) techniques, aimed to assess in real time the vigilance level of an ATCo dealing with a highly automated human–machine interface and to use this measure to adapt the level of automation of the interface itself. The system has been tested on 14 professional ATCos performing two highly realistic scenarios, one with the system disabled and one with the system enabled. The results confirmed that (i) long high automated tasks induce vigilance decreasing and OOTL-related phenomena; (ii) EEG measures are sensitive to these kinds of mental impairments; and (iii) AA was able to counteract this negative effect by keeping the ATCo more involved within the operative task. The results were confirmed by EEG and ET measures as well as by performance and subjective ones, providing a clear example of potential applications and related benefits of AA.
Home Range and Nest Arrangement of the Siberian Flying Squirrel Pteromys volans in an Urban Edge, Northern Japan

ABSTRACT Forest fragmentation by urbanizing and developing roads and fields is common in Japan; thus, the Siberian flying squirrel (Pteromys volans) population may decrease in the future. This study examined the effects of urbanization on P. volans by clarifying their home ranges and nest arrangements. Ten flying squirrels were captured and radio-telemetrically tracked from 2005 to 2007 in Obihiro City in eastern Hokkaido (Northern Japan). Males had significantly larger 100% MCP (minimum convex polygon) areas (mean ± SD; 6.5 ± 5.1 ha) and 95% MCP areas (3.6 ± 1.3 ha) than females (1.8 ± 1.0 ha and 1.0 ± 0.5 ha, respectively). Also the core area of males (1.2 ± 1.1 ha) was larger than females (0.2 ± 0.2 ha), though this difference was not significant. Males and females used more nests located inside than outside of their core areas. Distance from the core area edge to the nest located outside the core was longer for males (78.6 ± 139.9 m) than for females (39.7 ± 39.9 m), but this difference was not significant. Siberian flying squirrels in urban forests likely show similar ecology with flying squirrels in consecutive forests; however, they might change their behavior to share resources with others because urban forests restrict nest and food resources.
The Plasma [Kynurenine]/[Tryptophan] Ratio and Indoleamine 2,3-Dioxygenase: Time for Appraisal

The plasma kynurenine to tryptophan ([Kyn]/[Trp]) ratio is frequently used to express or reflect the activity of the extrahepatic Trp-degrading enzyme indoleamine 2,3-dioxygenase (IDO). This ratio is increasingly used instead of measurement of IDO activity, which is often low or undetectable in immune and other cells under basal conditions, but is greatly enhanced after immune activation. The use of this ratio is valid in in vitro studies, eg, in cell cultures or isolated organs, but its ‘blanket’ use in in vivo situations is not, because of modulating factors, such as supply of nutrients; the presence of multiple cell types; complex structural and functional tissue arrangements; the extracellular matrix; and hormonal, cytokine, and paracrine interactions. Determinants other than IDO may therefore be involved in vivo. These are hepatic tryptophan 2,3-dioxygenase (TDO) activity and the flux of plasma-free Trp down the Kyn pathway. In addition, conditions leading to accumulation of Kyn, eg, inhibition of activities of Kyn monooxygenase and kynureninase, could lead to elevation of the aforementioned ratio. In this review, the origin of use of this ratio will be discussed, variations in extent of its elevation will be described, evidence against its indiscriminate use will be presented, and examining determinants other than IDO activity and their correlates will be proposed for future studies.
Nrf2 Negatively Regulates Type I Interferon Responses and Increases Susceptibility to Herpes Genital Infection in Mice

Herpes simplex virus-2 (HSV-2) is a leading cause of sexually transmitted infections for which no effective vaccines or prophylactic treatment currently exist. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor involved in the detoxification of reactive oxygen species (ROS) and has been more recently shown to regulate inflammatory and antiviral responses. Here, we evaluated the importance of Nrf2 in the control of HSV-2 genital infection, and its role in the regulation of HSV-induced innate antiviral immunity. Comparison of antiviral gene expression profile by RNA-sequencing analysis of wild type and Nrf2-mutant (Nrf2AY/AY) murine macrophages showed an upregulation at the basal level of the type I interferon-associated gene network. The same basal increased antiviral profile was also observed in the spleen of Nrf2−/− mice. Interestingly, the lack of Nrf2 in murine cells was sufficient to increase the responsiveness to HSV-derived dsDNA and protect cells from HSV-2 infection in vitro. Surprisingly, there was no indication of an alteration in STING expression in murine cells as previously reported in cells of human origin. Additionally, genetic activation of Nrf2 in Keap1−/− mouse embryonic fibroblasts increased HSV-2 infectivity and replication. Finally, using an in vivo vaginal herpes infection model, we showed that Nrf2 controlled early innate immune responses to HSV-2 without affecting STING expression levels. Nrf2−/− mice exhibited reduced viral replication that was associated with higher level of type I interferons in vaginal washes. Nrf2−/− mice also displayed reduced weight loss, lower disease scores, and higher survival rates than wild type animals. Collectively, these data identify Nrf2 as a negative regulator of the interferon-driven antiviral response to HSV-2 without impairing STING mRNA and protein expression levels in murine cells.