Miracle-dz/UniFAR-FTUs huggingface dataset:
CyberKnife for inoperable renal tumors: Canadian pioneering experience.

INTRODUCTION Stereotactic ablative body radiotherapy (SABR) is currently under study regarding its clinical application in management of patients with kidney tumors. CyberKnife can accurately deliver ablative tumor radiation doses while preserving kidney function. We report Canada's first use of CyberKnife SABR system in treating primary kidney tumors. MATERIALS AND METHODS Between January 2011 and February 2012, we treated three patients with renal tumors using CyberKnife SABR. Two patients had tumors in solitary kidney. The third patient had a recurrent tumor after two previous radiofrequency ablation treatments. Platinum seed fiducials were used for real time tumor tracking. Magnetic resonance imaging registration was used for tumor delineation in all cases. The patients were followed with regular renal scans and renal function tests. RESULTS The mean age was 79 years. Mean tumor size was 21.3 cm3. A dose of 39 Gy in 3 fractions was delivered. The post treatment follow up times were 15 months, 13 months and 12 months. Local control was obtained in all three patients. No acute or chronic toxicity was reported. Kidney functions remained unaffected after treatment. CONCLUSION CyberKnife is technically feasible for treatment of medically inoperable renal tumors or tumors in a solitary kidney.
Overexpression of the NADP+-specific isocitrate dehydrogenase gene (icdA) in citric acid-producing Aspergillus niger WU-2223L

In the tricarboxylic acid (TCA) cycle, NADP+-specific isocitrate dehydrogenase (NADP+-ICDH) catalyzes oxidative decarboxylation of isocitric acid to form α-ketoglutaric acid with NADP+ as a cofactor. We constructed an NADP+-ICDH gene (icdA)-overexpressing strain (OPI-1) using Aspergillus niger WU-2223L as a host and examined the effects of increase in NADP+-ICDH activity on citric acid production. Under citric acid-producing conditions with glucose as the carbon source, the amounts of citric acid produced and glucose consumed by OPI-1 for the 12-d cultivation period decreased by 18.7 and 10.5%, respectively, compared with those by WU-2223L. These results indicate that the amount of citric acid produced by A. niger can be altered with the NADP+-ICDH activity. Therefore, NADP+-ICDH is an important regulator of citric acid production in the TCA cycle of A. niger. Thus, we propose that the icdA gene is a potentially valuable tool for modulating citric acid production by metabolic engineering. Graphical Abstract We revealed that NADP+-specific isocitrate dehydrogenase (NADP+-ICDH) is an important regulator of citric acid production in Aspergillus niger by constructing NADP+-ICDH gene (icdA)-overexpressing strain (OPI-1) using strain WU-2223L as a host.
Association between an MHC class II allele and clearance of hepatitis B virus in the Gambia.

BACKGROUND The course of hepatitis B virus (HBV) infection does not appear to be determined by variations in viral virulence and may be influenced by the host immune response. We studied the distribution of human leukocyte antigens in children and adult men in the Gambia who spontaneously recovered from HBV infection as compared with the distribution of these antigens in subjects with persistent infection. METHODS In a two-stage, case-control study, we analyzed the frequency of MHC class I antigens and class II haplotypes in people with either transient or persistent HBV infection. MHC class I typing was performed by microlymphocytotoxicity assays. MHC class II typing was performed with analysis of restriction-fragment-length polymorphisms (RFLPs), supplemented by other techniques. RESULTS In the first stage (the study of children up to the age of 10 years), the RFLP pattern 25-1, which includes the class II allele HLA-DRB1*1302, was found in 58 of 218 subjects with transient HBV infection (26.6 percent) and 30 of 185 subjects with persistent infection (16.2 percent) (relative risk of carrying the 25-1 pattern in the persistently infected group as compared with the transiently infected group, 0.53; 95 percent confidence interval, 0.32 to 0.90; P = 0.012). In the second stage (the study of adults), HLA-DRB1*1302 was found in 50 of 195 subjects with transient HBV infection (25.6 percent) and in 3 of 40 subjects with persistent infection (7.5 percent) (relative risk, 0.24; 95 percent confidence interval, 0.04 to 0.80; P = 0.012). The RFLP pattern 13-2, which includes the class II allele DRB1*1301, was less frequent in children with persistent infection than in those with transient infection, an association that was neither confirmed nor excluded by the data on adults. Possible associations with HLA class I antigens found in children were not supported by the data on adults. CONCLUSIONS The MHC class II allele DRB1*1302 was associated with protection against persistent HBV infection among both children and adults in the Gambia.
Numerosol A–D, New Cembranoid Diterpenes from the Soft Coral Sinularia numerosa

Four new cembrane-type diterpenes; numerosol A–D (1–4); along with a known steroid; gibberoketosterol (5); were isolated from the Taiwanese soft coral Sinularia numerosa. The structures of these metabolites were determined by extensive analysis of spectroscopic data. Gibberoketosterol (5) exhibited cytotoxicity against P-388 (mouse lymphocytic leukemia) cell line with an ED50 of 6.9 μM.
Digital style history: the development of graphic design on the Internet

Since the appearance of the phenomenon of web design in the early 1990s, numerous ways of describing, viewing and instrumentalising this new design area have developed. However, so far few attempts have been made to develop analytic and reflective approaches to web design as an aesthetic phenomenon. The aim of this article is from an art historical perspective to focus on WWW as a graphic-aesthetic medium that can be examined as an object or work, and whose development can be described within the framework of design history. The main goal is to show how the study of the graphic design development of the Internet may benefit by employing a central concept from art and design theory, the concept of style. This is used as a dynamic principle for structuring, both in the description and analysis of web design and as a point of departure for a discussion of visual aesthetics and the context-dependent experiences of looking at web design.
Acute rejection in low‐toxicity regimens: clinical impact and risk factors in the Symphony study

Frei U, Daloze P, Vítko Š, Klempnauer J, Reyes‐Acevedo R, Titiz I, Fricke L, Bernasconi C, Ekberg H. Acute rejection in low‐toxicity regimens: clinical impact and risk factors in the Symphony study. Clin Transplant 2010: 24: 500–509. © 2009 John Wiley & Sons A/S.
Concomitant Anterior Repair, Preoperative Prolapse Severity, and Anatomic Prolapse Outcomes After Vaginal Apical Procedures

Objective The aim of the study was to compare anterior and overall prolapse prevalence at 1 year in surgical participants with or without concomitant anterior repair (AR) at the time of sacrospinous ligament fixation (SSLF) or uterosacral ligament suspension (ULS). Methods This is a secondary analysis of two surgical trials; concomitant AR was performed at surgeon's discretion. Anterior anatomic success was defined as pelvic organ prolapse quantification of prolapse point Ba ⩽0 and overall success was defined as pelvic organ prolapse quantification points Ba, Bp, and C ⩽0 at 12 months. Results Sixty-three percent (441/701) of the participants underwent concomitant AR and were older, more often postmenopausal, had previous hysterectomy, and had higher-stage anterior, but not apical prolapse. Anterior anatomic success was marginally but statistically better in the combined group (SSLF and ULS) with concomitant AR (82% vs 80%, P = 0.03). In subanalyses, the improvement in anatomic support with AR was observed only in the SSLF subgroup (81% vs 73%, P = 0.02) and mostly in the SSLF subgroup with higher preoperative stage (74% vs 57%, P = 0.02). Anterior repair did not improve success rates in participants with lower-stage prolapse or undergoing ULS. Overall success rates were similar to anterior anatomic success rates. Participants with higher-stage preoperative anterior prolapse had significantly lower success rates. Conclusions In the absence of clinical trial data, this analysis suggests an AR should be considered for women with higher-stage prolapse undergoing an SSLF. Preoperative prolapse severity is a strong predictor of poor anatomic outcomes with native tissue vaginal apical surgeries.
The effect of cardiopulmonary bypass in coronary artery bypass surgeries (on-pump versus off-pump) on erectile function and endothelium-derived nitric oxide levels.

PURPOSE To investigate the effects of on-pump and off-pump coronary artery bypass grafting (CABG) on the erectile function and endothelium-derived nitric oxide (eNO) levels. MATERIALS AND METHODS Twenty-eight consecutive patients were randomized into two groups depending on use of cardiopulmonary bypass in CABG surgery. The erectile function was evaluated by using the IIEF-5 questionnaire. The plasma eNO levels were determined at baseline and after reactive hyperemia before and after surgery. Blood was collected in one minute after cuff deflation from the radial artery on the same side. RESULTS After CABG surgery the mean IIEF-5 score increased insignificantly over baseline from 14.8 to 15.8 (p = 0.29) and 12.4 to 14.3 (p = 0.11) after on-pump and off-pump CABG surgeries, respectively. The baseline plasma NO levels before surgery were 18.16 ± 7.63 nmol/L in on-pump and 21.76 ± 11.08 nmol/L in off-pump CABG. After reactive hyperemia the plasma NO levels were 22.14 ± 10.52 nmol/L in on-pump and 21.49 ± 9.13 nmol/L in off-pump CABG before the surgery. The difference in the plasma NO levels before surgery was not significant (p = 0.51). Two hours after surgery, the difference of the plasma NO levels at baseline (24.44 ± 12.31 on -pump and 20.58 ± 6.74 nmol/L off-pump CABG) and after reactive hyperemia (35.55 ± 23.54 nmol/L on-pump and 23.00 ± 15.40 nmol/L off-pump CABG) were not significantly different from each other (p = 0.11). CONCLUSIONS Patients who had on-pump or off-pump CABG surgeries had higher IIEF-5 scores. Nevertheless, the improvement was insignificant in both groups. Meanwhile, on-pump or off-pump CABG surgeries did not have significant effect on plasma eNO levels.
Urinary Incontinence among a 65-Year Old Swedish Population: Medical History and Psychosocial Consequences

Urinary incontinence (UI) is a disability caused by an impairment, which can lead to a handicap of importance for nursing care. This means that UI is not only a practical-medical concern but also a socio-economic problem. The purpose of the study was to determine the prevalence of UI among 65 year-olds in a Swedish Health Care District and to compare gender differences concerning medical history and psychosocial consequences. In a Primary Health Care District, a questionnaire pertaining to UI was mailed to all women and men 65 years of age (N=458). A total of 91% (n=419) was sufficient for data analysis, which was performed by descriptive and inferential statistics. It was found that 28% (n=61) of the women and 9% (n=21) of the men were afflicted with UI. Women reported significantly more urge incontinence (p<.05) as well as stress incontinence (p<.05). Information from the health service about UI had been given to 46% (n=28) of the women and 33% (n=7) of the men. The strongest reason reported, both in women (42%, n=26) and men (40%, n=8), for not seeking help from the health service was that UI was a normal condition for people of their age. Most of the women had to urinate at least twice per night (42%) compared to once per night (44%) for the men. It is important to establish a UI clinic at every main Primary Health Care Centre which builds on nursing care and whose aim is to inform the general public that UI is a common problem, that it leads to psychosocial consequences, and that the health service can offer active rehabilitation interventions.
Scoring system for evaluating the stress to cattle of commercial loading and unloadind

The loading and unloading of cattle for road transport is stressful but the stress is difficult to evaluate in terms of the welfare of the cattle. Over one year, 40 normal commercial journeys in northern Spain were analysed in terms of time limits and behavioural events in order to design an objective method for assessing the stresses imposed on the animals, and a scoring method was developed for assessing the welfare of the animals during loading and unloading. Several definitions of time intervals were assessed to calculate a time score per animal, and easily observable behavioural events were scored and combined with the time score to obtain a total loading/unloading score. More than half of the loadings and unloadings involved turns, slips and vocalisations. Mounts and bouts of fighting were infrequent and balks and falls were significantly more frequent during loading than unloading. The plasma concentrations of cortisol, glucose and lactate, the activity of creatine kinase and the pH of the meat 24 hours after the animals were slaughtered were also measured. The results indicated that loading was more stressful than unloading and that higher scores implied significantly higher levels of stress.
Long-Term Cost of Stroke Subtypes among Medicare Beneficiaries

Background and Purpose: The economic impact of hemorrhagic stroke, including subarachnoid hemorrhage (SAH) and intracerebral hemorrhage (ICH), has not been well characterized compared to the more prevalent ischemic stroke (IS). Methods: Patients diagnosed with SAH, ICH or IS in 1997 were identified in a 5% national random sample of all Medicare beneficiaries. Medical care patterns and associated Medicare reimbursements were analyzed from one year prior to the index event through four years following that event. Results: 11,430 patients were identified with SAH (n = 342), ICH (n = 1,957) or IS (n = 9,131). Average Medicare expenditures, from the initial event through four years, were USD 48,327 for SAH, USD 38,023 for ICH and USD 39,396 for IS. Conclusions: Long-term healthcare costs of SAH and ICH are substantial. With the expected increase in the elderly population over the coming decades, these results emphasize the need for effective preventive and acute medical care.
Necrotizing tracheobronchitis.

To the Editor. — We read with interest the report by Mimouni et al1 of a case of necrotizing tracheobronchitis. We agree that survival is possible for patients with necrotizing tracheobronchitis. We recently reported eight cases of necrotizing tracheobronchitis diagnosed prospectively (between January 1983 and September 1984) and 12 cases identified retrospectively.2 From these cases, we identified characteristic clinical findings. These findings, which follow, should alert clinicians early enough to institute therapy for necrotizing tracheobronchitis. Necrotizing tracheobronchitis supervenes on a previously stable course in an infant requiring mechanical ventilation.
Gingival/soft tissue abscess following subepithelial connective tissue graft for root coverage: report of three cases.

BACKGROUND The subepithelial connective tissue graft (SCTG) is a predictable technique for root coverage. The small number of potential postoperative problems make SCTG the current treatment of choice for reversing gingival recession. METHODS The purpose of this article is to report three cases of complications after the use of an SCTG where an abscess occurred following the initial healing phase. The surgical technique that was used and the treatment that was provided for the abscesses are reported, and the possible causes of these complications are discussed. RESULTS The abscesses were treated conservatively, and no recurrence has been observed after treatment. CONCLUSIONS Possible causes of the abscesses include "stitch abscess" or reaction to the suture material used for the submerged sutures, or a possible role of epithelium implanted during the procedure. The clinical outcome of the root coverage procedure was not affected.
Proximate causes and fitness consequences of hatching failure in lesser kestrels Falco naumanni

Hatching failure is a pervasive phenomenon in birds, but factors affecting hatchability remain poorly understood. We studied proximate causes and fitness consequences of hatching failure in a long-monitored population of the colonial lesser kestrel Falco naumanni . We investigated whether hatchability was related to clutch characteristics, parental traits, and social or environmental features. Hatching failure represents a cost for the parents in terms of immediate fitness, since it reduced both their number of young fledged and recruits in the breeding population, even when controlling for clutch size. Hatching failure showed a non-linear relationship with clutch size, clutches of four eggs showing higher levels of hatching success than larger or smaller clutches. Hatchability could therefore play a role in the evolution of optimal clutch size in this species, at least constraining the maximum number of eggs the parents can afford to incubate. Contrary to most studies, the mean volume of the clutch and the individual egg volume were negatively related to hatching failure, indicating that large eggs have thermoregulatory and/or nutritional advantages. Mean daily maximum temperature during incubation affected hatching success negatively, but only for females in poor condition. This result seems to indicate that females are reluctant to jeopardize their own condition, but instead sacrifice incubation effort by paying the costs of a lower hatching success in circumstances of high temperatures. There was no evidence that hatching failure was related to the intrinsic properties of individuals or genetic similarity between the parents as indicated by low repeatabilities of: (1) males that bred with different females, (2) females that bred with different males, and (3) pairs breeding together in different years. Neither colony size nor subpopulation size affected hatchability. All these findings show how hatching failure is simultaneously influenced by several factors acting in a complex way, which could in part explain the apparently conflicting conclusions of empirical or even experimental studies carried out to date.
Novel Gene Product of Thogoto Virus Segment 6 Codes for an Interferon Antagonist

ABSTRACT Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome of six negative-stranded RNA segments. The sixth segment encodes two different transcripts: a spliced transcript that is translated into the matrix protein (M) and an unspliced transcript. Here, we report that the unspliced transcript encodes an elongated form of M named ML. A THOV isolate deficient in ML expression was an efficient interferon inducer, whereas ML-expressing wild-type strains were poor interferon inducers. These results were confirmed with recombinant THOVs rescued from cDNAs. Expression of ML efficiently suppressed activation of the beta interferon promoter by double-stranded RNA. These results indicate that ML is an accessory protein that functions as a potent interferon antagonist by blocking transcriptional activation of alpha/beta interferons.
Cefdinir nanosuspension for improved oral bioavailability by media milling technique: formulation, characterization and in vitro–in vivo evaluations

Abstract Cefdinir (Cef) is an orally active Biopharmaceutics Classification System (BCS) class IV drug with incomplete absorption and low bioavailability (16–21%). The aim of this investigation was to develop nanosuspensions (NS) of Cef to improve its oral bioavailability. Cef NS were prepared by the media milling technique using zirconium oxide beads as the milling media. Cef NS were characterized by particle size, Scanning Electron Microscopy, Differential Scanning Calorimetry, X-Ray Diffraction pattern and evaluated for saturation solubility, in vitro release studies, ex vivo permeability studies and in vivo bioavailability studies. The particle size and zeta potential were found to be 224.2 ± 2.7 nm and −15.7 ± 1.9 mV, respectively. Saturation solubility of NS was found to be 1985.3 ± 10.2 µg/ml which was 5.64 times higher than pure drug (352.2 ± 6.5 µg/ml). The DSC thermograms and XRD patterns indicated that there was no interaction between drug and excipients and that the crystallinity of Cef remained unchanged after media milling process. Results of in vitro release studies and ex vivo permeation studies showed improved drug release of 88.2 1 ± 2.90 and 83.11 ± 2.14%, respectively, from NS after 24 h as compared to drug release of 54.09 ± 2.54 and 48.2 1 ± 1.27%, respectively, from the marketed suspension (Adcef). In vivo studies in rats demonstrated a 3-fold increase in oral bioavailability from the NS in comparison to marketed suspension. The results of this investigation conclusively show that the developed nanosuspension of Cef exhibited improved solubility, dissolution and permeation which led to a significant enhancement in its oral bioavailability.
Epidermal Patterns of the Lemma in Some Fossil and Living Grasses and Their Phylogenetic Significance

Morphological study of fossil grass anthoecia of Berriochloa and Nassella collected from Miocene-Pliocene strata in Kansas has revealed well-preserved epidermal structure. This seems to be the first micromorphological information known from fossil grass floral bracts. The epidermal pattern on the lemma in the fossils and their living counterparts are evidence in support of the view that the North American species of Stipa of the section Hesperostipa Elias and species of Piptochaetium have a common ancestry in Berriochloa, and that species of both taxa have been distinct from species of the Nassella, Oryzopsis, and other Stipa since at least the Miocene or Pliocene.
Power splitting protocol design for the cooperative NOMA with SWIPT

In this paper, we consider the simultaneous wireless information and power transfer assisted cooperative nonorthogonal multiple access (SWIPT-CNOMA) system, in which the near NOMA users with strong channel conditions serve as energy harvesting (EH) relays to help the far NOMA users with poor channel conditions. For the considered system, a new power splitting (PS) protocol for EH relay is proposed and its impact on the outage performance of both near and far NOMA users is studied. The analytical expressions of both outage probability and system throughput are further derived for delay-sensitive transmission mode. Simulation results are provided to verify the derived results and reveal the advantages of the proposed protocol.