Miracle-dz/UniFAR-FTUs huggingface dataset:
Subcortical white matter lesions and functional impairment in geriatric depression

Geriatric depression is associated with significant functional impairment. There is also growing evidence linking vascular brain changes to depression in late life. We sought to examine the relationship between cerebrovascular disease and impairment in basic activities of daily living (BADL) and instrumental activities of daily living (IADL) in a group of older depressives. The sample consisted of 224 depressed adults aged 60 years and above enrolled in Duke’s Mental Health Clinical Research Center. All subjects had unipolar major depression and were free of other major psychiatric and neurological illness, including dementia. In a structured interview, subjects reported their medical history and ability to perform both BADL and IADL. Geriatric psychiatrists assessed cognition using the Mini Mental State Examination (MMSE) and depression severity using the Montgomery Asberg Depression Rating Scale (MADRS). Subjects had a standardized magnetic resonance imaging (MRI) brain scan. MRI scans were processed using a semi‐automated method to determine volumes of subcortical white matter lesions (WML) and subcortical gray matter lesions (GML). Logistic regression was used to examine effects of WML and GML controlling for demographic and clinical factors. Greater volume of WML was associated with impairment in both BADL and IADL, while GML was associated with IADL impairment. In logistic models, WML remained significantly associated with IADL after controlling for the effects of age, gender, depression severity, and medical comorbidity. We concluded that white matter lesions are independently associated with functional impairment. Further studies are needed to understand how these lesions affect function, e.g., through effects on cognition or motor skills. Depression and Anxiety 15:23–28, 2002. © 2002 Wiley‐Liss, Inc.
BETRIEBSVERHALTEN UND ABGASEMISSION EINES VERBRENNUNGSMOTORS MIT DEM PORSCHE-SCHICHTLADE-KAMMER-SYSTEM

DER PORSCHE-SCHICHTLADE-KAMMER-SYSTEM-MOTOR (SKS) STELLT EINE VERBRAUCHS- UND ABGASGUENSTIGE ALTERNATIVE ZUM HERKOEMMLICHEN OTTOMOTOR MIT ABGASNACHBEHANDLUNG DAR. DURCH DAS SYSTEM MIT UNTERTEILTEM BRENNRAUM UND DREISTUFIGER VERBRENNUNG WURDE EINE OPTIMIERUNG FUER JEDE EINZELNE VERBRENNUNGSPHASE ANGESTREBT. DIE AM EINZYLINDER-FORSCHUNGSMOTOR GEMESSENEN KONZENTRATIONEN DER EINZELNEN ABGASKOMPONENTEN ZEIGEN DIE GRUNDSAETZLICH GLEICHEN ABHAENGIGKEITEN WIE BEIM OTTOMOTOR, ABER IN UNTERSCHIEDLICHEN VERHAELTNISSEN ZUEINANDER. DER SAUGROHREINSPRITZZEITPUNKT DES EINGEBRACHTEN BRENNSTOFFES IN EINEM GROSSEN VERSTELLBEREICH HAT KEINEN EINFLUSS AUF DAS BETRIEBSVERHALTEN UND DIE ABGASEMISSION. ALS WESENTLICH FUER DAS BETRIEBSVERHALTEN ERWEIST SICH DER TEMPERATURZUSTAND DER NEBENKAMMER, WOBEI SICH EINE ERHOEHUNG DER WANDTEMPERATUR UEBER DIE VERBESSERUNG DER GEMISCHAUFBEREITUNG GUENSTIG AUF LEISTUNG UND VERBRAUCH AUSWIRKT. DIE GESAMTLUFTZAHL DES SCHICHTLADEMOTORS IST DURCH DIE LUFTZAHLEN DER NEBENKAMMER UND DES HAUPTRAUMES BESTIMMT . BEI GUTEN VORAUSSETZUNGEN HAT DIE LUFTZAHL IN EINEM BREITEN BEREICH NICHT MEHR JENE BEDEUTUNG FUER DEN MOTORBETRIEB UND DIE ABGASEMISSION WIE BEIM HERKOEMMLICHEN OTTOMOTOR. DER ZUENDZEITPUNKT HAT BEIM SCHICHTLADEMOTOR EINEN WESENTLICH GUENSTIGEREN EINFLUSS ALS BEIM OTTOMOTOR. GROSSE BEDEUTUNG BEIM SCHICHTLADEMOTOR HAT DIE GESTALTUNG DES SCHUSSKANALS. DIE VORGESTELLTE VARIANTE ERGIBT BEI ETWA GLEICHEN VERBRAEUCHEN ETWAS GUENSTIGERE CO- UND HC-EMISSIONEN ALS DER OTTOMOTOR, ABER WESENTLICH NIEDRIGERE NO-EMISSIONEN.
Novel Bifunctional Nucleases, OmBBD and AtBBD1, Are Involved in Abscisic Acid-Mediated Callose Deposition in Arabidopsis1[W][OA]

Screening of the expressed sequence tag library of the wild rice species Oryza minuta revealed an unknown gene that was rapidly and strongly induced in response to attack by a rice fungal pathogen (Magnaporthe oryzae) and an insect (Nilaparvata lugens) and by wounding, abscisic acid (ABA), and methyl jasmonate treatments. Its recombinant protein was identified as a bifunctional nuclease with both RNase and DNase activities in vitro. This gene was designated OmBBD (for O. minuta bifunctional nuclease in basal defense response). Overexpression of OmBBD in an Arabidopsis (Arabidopsis thaliana) model system caused the constitutive expression of the PDF1.2, ABA1, and AtSAC1 genes, which are involved in priming ABA-mediated callose deposition. This activation of defense responses led to an increased resistance against Botrytis cinerea. atbbd1, the knockout mutant of the Arabidopsis ortholog AtBBD1, was susceptible to attack by B. cinerea and had deficient callose deposition. Overexpression of either OmBBD or AtBBD1 in atbbd1 plants complemented the susceptible phenotype of atbbd1 against B. cinerea as well as the deficiency of callose deposition. We suggest that OmBBD and AtBBD1 have a novel regulatory role in ABA-mediated callose deposition.
A Preliminary Examination of the Effects of Pretreatment Relationship Satisfaction on Treatment Outcomes in Cognitive-Behavioral Conjoint Therapy for PTSD.

OBJECTIVE The primary goal of the present study was to investigate whether pre-treatment relationship satisfaction predicted treatment drop-out and posttraumatic stress disorder (PTSD) symptom outcomes within a trial of cognitive-behavioral conjoint therapy (CBCT) for PTSD (Monson & Fredman, 2012). Additionally, we examined the influence of pre-treatment relationship distress on relationship outcomes. METHOD Thirty-seven patients and their intimate partners who participated in a course of CBCT for PTSD were assessed for PTSD symptoms with the Clinician-Administered PTSD Scale and PTSD Checklist, and for intimate relationship functioning with the Dyadic Adjustment Scale. CBCT for PTSD is a conjoint therapy designed to improve PTSD symptoms and enhance relationship functioning. Patients had to meet diagnostic criteria for PTSD to be included in the study; however, couples were not required to be in distressed relationships to receive treatment. RESULTS Neither patients' nor partners' pre-treatment relationship satisfaction, nor their interaction, predicted treatment drop-out (ORs = .97-1.01) or completing patients' post-treatment PTSD symptom severity (sr2 ≤ .03). However, participants who were in distressed relationships prior to treatment made greater gains in relationship satisfaction compared with those who began treatment in more satisfied relationships (g = 1.02). CONCLUSIONS Among patients receiving CBCT for PTSD, treatment drop-out and improvements in PTSD symptoms may be independent of pre-treatment relationship functioning, whereas improvements in relational functioning may be greater among those distressed prior to treatment.
Lifestyle behaviours and weight among hospital-based nurses.

AIMS The purpose of this study was to (i) describe the weight, weight-related perceptions and lifestyle behaviours of hospital-based nurses, and (ii) explore the relationship of demographic, health, weight and job characteristics with lifestyle behaviours. BACKGROUND The obesity epidemic is widely documented. Worksite initiatives have been advocated. Nurses represent an important part of the hospital workforce and serve as role models when caring for patients. METHODS A sample of 194 nurses from six hospitals participated in anthropometric measurements and self-administered surveys. RESULTS The majority of nurses were overweight and obese, and some were not actively involved in weight management behaviours. Self-reported health, diet and physical activity behaviours were low, although variable by gender, age and shift. Reports of co-worker norms supported low levels of healthy behaviours. CONCLUSIONS Findings reinforce the need to address the hospital environment and culture as well as individual behaviours for obesity control. IMPLICATIONS FOR NURSING MANAGEMENT Nurse managers have an opportunity to consider interventions that promote a climate favourable to improved health habits by facilitating and supporting healthy lifestyle choices (nutrition and physical activity) and environmental changes. Such efforts have the potential to increase productivity and morale and decrease work-related disabilities and improve quality of life.
ANTIBODY DYNAMICS IN HOLSTEIN FRIESIAN HEIFERS VACCINATED WITH BRUCELLA ABORTUS STRAIN 19, USING SEVEN SEROLOGICAL TESTS

ABSTRACT The serological response induced by Brucella abortus strain 19 was evaluated in 52 Holstein females from a brucellosis-free herd using seven serological tests. Each calf was vaccinated at an age of 4 and 8 months old with 3 × 1010 CFU B. abortus S19 and the antibody response was determined as the proportion of positive results to each test. The antibody dynamics, measured with the buffered plate antigen (BPA) test and the rapid automated presumptive (RAP) test, were similar. The proportion of positive reactions in these tests reached 100% one week after vaccination and remained at this level for seven weeks, after which the proportion of positive samples slowly declined to 8% (BPA) and 2% (RAP) at week 50. The response in the indirect enzyme immunoassay (i-ELISA) was similar, but shorter than that observed with the BPA/RAP. The antibody dynamic, measured using the seroagglutination test (SAT) in parallel with the 2-mercaptoethanol (2-Me) test and the complement fixation test (CFT) were similar to the RAP/BPA, but of slightly shorter duration. The competitive ELISA (c-ELISA) was positive in all animals for 3 weeks, followed by a rapid decline. The fluorescence polarization assay (FPA) reached a maximum of 68.5% positive animals at week 4 and then declined. Based on these data, the c-ELISA and FPA discriminated residual antibody activity due to vaccination more efficiently than the other tests.
Significance of the third tRNA binding site, the E site, on E. coli ribosomes for the accuracy of translation: an occupied E site prevents the binding of non‐cognate aminoacyl‐tRNA to the A site.

The E site (exit site for deacyl‐tRNA) has been shown to be allosterically linked to the A site (aminoacyl‐tRNA binding site), in that occupation of the E site reduces the affinity of the A site, and vice versa, whereas the intervening peptidyl‐tRNA binding site (P site) keeps its high affinity. Here the question is analysed of whether or not the low affinity state of the A site caused by an occupied E site is of importance for the ribosomal accuracy of the aminoacyl‐tRNA selection. In a poly(U) dependent system with high accuracy in poly(Phe) synthesis, the acceptance of the cognate ternary complex Phe‐tRNA–EF‐Tu–GTP (which has the correct anticodon with respect to the codon at the A site) was compared with the competing acceptance of ternary complexes with near‐cognate Leu‐tRNA(Leu) (which has a similar anticodon) or non‐cognate Asp‐tRNA(Asp) (which has a dissimilar anticodon), by monitoring the formation of AcPhePhe, AcPheLeu or AcPheAsp, respectively. Cognate (but not near‐cognate) occupation of the E site reduced synthesis of the ‘wrong’ dipeptide AcPheLeu only marginally relative to that of the cognate AcPhe2, whereas the formation of AcPheAsp was decreased as much as 14‐fold, thereby reducing it to the background level. It follows that the allosteric interplay between E and A sites, i.e. the low affinity of the A site induced by the occupation of the E site, excludes the interference of non‐cognate complexes in the decoding process and thus reduces the number of aminoacyl‐tRNA species competing for A site binding by an order of magnitude. (ABSTRACT TRUNCATED AT 250 WORDS)
p202, an Interferon-inducible Modulator of Transcription, Inhibits Transcriptional Activation by the p53 Tumor Suppressor Protein, and a Segment from the p53-binding Protein 1 That Binds to p202 Overcomes This Inhibition*

p202, an interferon-inducible murine protein, is a member of the “200 family” of proteins and is primarily nuclear. p202 is a modulator of transcription; it binds several transcription factors, including NF-κB p50 and p65, AP-1 c-Fos and c-Jun, and E2F1, and inhibits their transcriptional activity. p202 also binds pRb, the retinoblastoma protein, and if overexpressed it retards cell proliferation. Here we report that using the yeast two-hybrid assay we found that p202 bound the murine homolog of the human p53-binding protein 1 (53BP1), a protein shown to interact with the DNA binding domain of the p53 tumor suppressor protein. p202 bound a 98amino acid segment from 53BP1. This binding was inhibited by the replacement in p202 of a histidine (from the M(F/L)HATVA(T/S) sequence that is conserved among all of the 200 family proteins) by phenylalanine. We also report that overexpression of p202 inhibited the p53-dependent expression of reporter genes containing p53-activable segments from the mdm2 and p21 genes, whereas a decrease in the p202 level (in consequence of the expression of 202 antisense RNA) resulted in an increase in the p53-dependent expression of these reporters. Expression of the 53BP1 segment binding to p202 overcame the inhibition by overexpressed p202 of the transcription of reporters mediated by the p53 or the AP-1 transcription factors and of the proliferation of yeast.
Comparison of fine‐needle aspiration cytology and core biopsy for diagnosis of breast cancer

Both fine‐needle aspiration (FNA) cytology and core biopsy are useful in the diagnosis of breast cancer. In order to compare the sensitivities of these procedures, we reviewed 209 patients with breast cancer who had either FNA, core biopsy, or both, and also either mastectomy or lumpectomy. Sensitivities for FNA and core biopsies for diagnosing breast cancer were calculated and compared. Sensitivity for FNA or core biopsies interpreted as either atypical or malignant was 93.8% for FNA and 90.1% for core biopsy (P > 0.05). Sensitivity for FNA or core biopsies interpreted as malignant was 65.4% for FNA and 88.7% for core biopsy (P < 0.0001). Sensitivities of FNA interpreted as either atypical or malignant were 92.4% for FNA performed by pathologists and 100% for FNA by nonpathologists (P >0.05). Sensitivities of FNA interpreted as malignant were 75.8% for FNA by pathologists and 20.0% for FNA by nonpathologists (P < 0.00001). Both FNA and core biopsies are sensitive procedures for the detection of breast cancer. There was no significant difference between sensitivity of FNA and core biopsies interpreted as either atypia or malignancy, although the sensitivity of core biopsies interpreted as unequivocal malignancy was greater than that of FNA. FNAs performed by pathologists were more sensitive than FNAs performed by nonpathologists in making an unequivocal diagnosis of breast cancer. Diagn. Cytopathol. 24:421–425, 2001. © 2001 Wiley‐Liss, Inc.
Relationship of incident glaucoma versus physical activity and fitness in male runners.

PURPOSE To assess the dose-response relationship of vigorous physical activity (running distance, km x d(-1)) or cardiorespiratory fitness (meters-per-second pace during a 10-km footrace) to the risk for incident glaucoma. DESIGN Prospective epidemiologic cohort study. METHODS Participant-reported, physician-diagnosed incident glaucoma was compared with distance run per week and 10-km footrace performance in a cohort of 29,854 male runners without diabetes followed prospectively for 7.7 yr. The survival analyses were adjusted for age, hypertension, current and past cigarette use, and intakes of meat, fish, fruit, and alcohol. RESULTS Two hundred incident glaucoma cases were reported during follow-up. The risk for reported glaucoma decreased 37% per meter per second increment in a 10-km race performance (P = 0.005). Relative to the least fit men (i.e., slowest, < or = 3.5 m x s(-1)), the risk for incident-reported glaucoma declined 29% in those who ran 3.6-4.0 m x s(-1) (P = 0.06), 54% for those who ran 4.1-4.5 m x s(-1) (P = 0.001), 51% for those who ran 4.6-5.0 m x s(-1) (P = 0.04), and glaucoma was nonexistent among the 781 men who exceeded 5.0 m x s(-1) (P = 0.03). The risk for incident, reported glaucoma decreased 5% per kilometer per day run at baseline (P = 0.04), which remained significant when adjusted for the 10-km race performance (5% reduction per kilometer per day, P = 0.04), and both body mass index and race performance (P = 0.04). Baseline hypertension was unrelated to the incident glaucoma. CONCLUSIONS These data provide preliminary evidence that vigorous physical activity may reduce glaucoma risk, which, in the absence of medical record validation, could represent ocular hypertension in addition to frank glaucoma. Additional follow-up with validation is needed to identify the type of glaucoma affected.
Squamous cell carcinoma of the maxillary sinus: a Tata Memorial Hospital experience.

BACKGROUND The optimal treatment of maxillary sinus carcinoma remains to be defined and there is a paucity of Indian studies on the subject. AIMS To present experience of management of squamous cell carcinoma of the maxillary sinus treated with curative intent at a single institution. SETTINGS AND DESIGN Retrospective study of patients with squamous cell carcinoma of the maxillary sinus who presented between 1994 to 1999. MATERIALS AND METHODS The records of 73 patients with squamous cell carcinoma of the maxillary sinus were analyzed. Sixty-two patients were evaluable. Forty patients (65%) were treated with surgery followed by postoperative radiotherapy, five patients (8%) were treated with radiotherapy alone, five patients (8%) were treated with surgery alone; 12 patients (19%) received chemotherapy. STATISTICAL ANALYSIS USED Statistical analysis was done using Kaplan-Meier method. RESULTS The majority of patients presented with locally advanced disease (52, 84%); nodal involvement was observed in five patients (8%). The most common site of recurrence was at the primary site, which was observed in 28 patients (45%) and regional failures occurred in 10 (16%). The 3 and 5-year overall survival was 38% and 35% and the disease free survival was 29% and 26% respectively. The 5-year overall survival after surgery and postoperative radiotherapy was 42%. CONCLUSIONS The majority of patients present with advanced disease resulting in poor outcomes to conventional treatment modalities. Locoregional tumor progression remains a significant pattern of failure. New approaches such as neoadjuvant or concomitant chemoradiotherapy with aggressive surgery need to be considered and evaluated in prospective studies.
Stress, Aggression, and Puberty: Neuroendocrine Correlates of the Development of Agonistic Behavior in Golden Hamsters

During puberty, agonistic behaviors undergo significant transitions. In golden hamsters, puberty is marked by a transition from play fighting to adult aggression. During early puberty, male golden hamsters perform play-fighting attacks. This response type is gradually replaced by adult attacks over the course of puberty. Interestingly, this behavioral transition does not appear to be controlled by changes in gonadal steroids. Instead, the shift from play fighting to adult aggression in male golden hamsters is driven by pubertal changes in glucocorticoid levels. Specifically, the transition from play fighting to adult aggression coincides with developmental increases in glucocorticoid levels, and external manipulations such as social stress or treatment with corticosteroid receptor agonists accelerate this behavioral shift. Moreover, the consequences of social stress differ greatly between juvenile and adult male golden hamsters. Although a single defeat during adulthood causes severe and long lasting behavioral and neuroendocrine consequences, socially subjugated juveniles show only transient behavioral effects. As such, it is likely that pubertal changes in the HPA axis are not only linked to the maturation of offensive responses but also determine the consequences of social stress. Combined, these studies in golden hamsters provide a novel mechanism for the development of agonistic behavior and suggest that age related differences in behavioral plasticity are mediated by the development of the HPA axis.
Synthesis of sphingosine is essential for oxidative stress-induced apoptosis of photoreceptors.

PURPOSE Oxidative stress is involved in inducing apoptosis of photoreceptors in many retinal neurodegenerative diseases. It has been shown that oxidative stress increases in photoreceptors the synthesis of ceramide, a sphingolipid precursor that then activates apoptosis. In several cell types, ceramide is converted by ceramidases to sphingosine (Sph), another apoptosis mediator; hence, this study was undertaken to determine whether Sph participates in triggering photoreceptor apoptosis. METHODS Rat retina neurons were incubated with [(3)H]palmitic acid and treated with the oxidant paraquat (PQ) to evaluate Sph synthesis. Sph was added to cultures with or without docosahexaenoic acid (DHA), the major retina polyunsaturated fatty acid and a photoreceptor survival factor, to evaluate apoptosis. Synthesis of Sph and sphingosine-1-phosphate (S1P), a prosurvival signal, were inhibited with alkaline ceramidase or sphingosine kinase inhibitors, respectively, before adding PQ, C(2)-ceramide, or Sph. Apoptosis, mitochondrial membrane polarization, cytochrome c localization, and reactive oxygen species (ROS) production were determined. RESULTS PQ increased [(3)H]Sph synthesis in photoreceptors and blocking this synthesis by inhibiting alkaline ceramidase decreased PQ-induced apoptosis. Addition of Sph induced photoreceptor apoptosis, increased ROS production, and promoted cytochrome c release from mitochondria. Although DHA prevented this apoptosis, inhibiting Sph conversion to S1P blocked DHA protection. CONCLUSIONS These results suggest that oxidative stress enhances formation of ceramide and its subsequent breakdown to Sph; ceramide and/or Sph would then trigger photoreceptor apoptosis. Preventing Sph synthesis or promoting its phosphorylation to S1P rescued photoreceptors, suggesting that Sph is a mediator of their apoptosis and modulation of Sph metabolism may be crucial for promoting photoreceptor survival.
MicroRNA-146b regulates hepatic stellate cell activation via targeting of KLF4.

Background. We previously identified miR-146b as being up-regulated during the development of hepatic fibrosis using deep sequencing technology and gene expression analysis. However, the roles and related mechanisms of miR-146b in hepatic stellate cells (HSCs), which are involved in fibrogenesis and fibrosis, have not been elucidated. RESULTS We report that miR-146b expression was increased in TGF-ß1-treated HSCs. TGF- ß1 enhanced α-SMA and COL1A1 protein expression in HSCs and stimulated proliferation of these cells compared with cells transfected with inhibitor NC. Conversely, miR-146b knock-down decreased α-SMA and COL1A1 expression and inhibited HSC proliferation. In addition, we found that miR-146b specifically regulated the translation of Krüppel-like factor 4 (KLF4) by targeting its 3' untranslated region. Forced expression of KLF4 inhibited TGF- ß1-induced enhancement of α-SMA and COL1A1 expression in HSCs, as well as proliferation of these cells. Moreover, miR-146b expression was negatively associated with KLF4 expression but positively associated with expression of α-SMA and COL1A1 during hepatic fibrosis. CONCLUSIONS Our findings demonstrate the participation of miR-146b as a novel upstream effector of HSC activation via direct targeting of KLF4. Thus, targeted transfer of miR-146b into HSCs could be a useful strategy for the treatment of hepatic fibrosis.