Miracle-dz/UniFAR-FTUs huggingface dataset:
Presence of high levels of non-degraded gliadin in breast milk from healthy mothers.

BACKGROUND Secretion of dietary antigens into breast milk has been extensively documented. The presence of these antigens is of relevance because they could be involved in the modulation of the immune response in neonates. The objective of this study is to determine the gliadin concentration in milk, colostrum, and serum samples from healthy lactating mothers on a normal diet. Gliadin levels in milk samples from a group of six mothers after a brief period of gluten restriction were also determined. The molecular weight of secreted gliadins was also analysed. METHODS Gliadin concentration was determined with a highly sensitive competitive enzyme-linked immunosorbent assay, modified so as to eliminate anti-gliadin antibody interference. The level of gliadin/IgA anti-gliadin immune complexes in milk, colostrum, and serum samples was determined. RESULTS Gliadin was detected in all 49 milk samples. Its concentration varied between 5 and 1200 ng/ml (mean, 178 ng/ml). In colostrum (n = 14) gliadin levels were higher (range, 28-9000 ng/ml; mean, 883 ng/ml), not being detectable in one case. Gliadin was detectable in 14 of 31 serum samples, in which levels were lower than in milk and colostrum samples (mean, 41 ng/ml). Neither a correlation between gliadin levels in milk, colostrum, and serum samples from the same subject nor a relation between gluten intake and gliadin concentration in milk samples from six subjects under a 3-day gluten-free diet could be found. Higher levels of immune complexes were observed in colostrum samples than in milk and serum samples. No correlation was detected between gliadin concentration and the level of immune complexes. The analysis of milk and colostrum samples by immunoblotting showed bands of immunoreactive gliadin presenting Mr similar to those of native proteins from wheat extracts. CONCLUSIONS Very high levels of gliadin were detected in milk samples from healthy mothers on an unrestricted diet. Gliadin levels were higher than those reported for dietary antigens in other studies. Breast milk contained non-degraded gliadins and gliadin/anti-gliadin IgA immune complexes.
Phosphorylation of Bcl10 Negatively Regulates T-Cell Receptor-Mediated NF-κB Activation

ABSTRACT Bcl10 (B-cell lymphoma 10) is an adaptor protein comprised of an N-terminal caspase recruitment domain and a C-terminal serine/threonine-rich domain. Bcl10 plays a critical role in antigen receptor-mediated NF-κB activation and lymphocyte development and functions. Our current study has discovered that T-cell activation induced monophosphorylation and biphosphorylation of Bcl10 and has identified S138 within Bcl10 as one of the T-cell receptor-induced phosphorylation sites. Alteration of S138 to an alanine residue impaired T-cell activation-induced ubiquitination and subsequent degradation of Bcl10, ultimately resulting in prolongation of TCR-mediated NF-κB activation and enhancement of interleukin-2 production. Taken together, our findings demonstrate that phosphorylation of Bcl10 at S138 down-regulates Bcl10 protein levels and thus negatively regulates T-cell receptor-mediated NF-κB activation.
Assessment of functional effects of unclassified genetic variants

Inherited predisposition to disease is often linked to reduced activity of a disease associated gene product. Thus, quantitation of the influence of inherited variants on gene function can potentially be used to predict the disease relevance of these variants. While many disease genes have been extensively characterized at the functional level, few assays based on functional properties of the encoded proteins have been established for the purpose of predicting the contribution of rare inherited variants to disease. Much of the difficulty in establishing predictive functional assays stems from the technical complexity of the assays. However, perhaps the most challenging aspect of functional assay development for clinical testing purposes is the absolute requirement for validation of the sensitivity and specificity of the assays and the determination of positive predictive values (PPVs) and negative predictive values (NPVs) of the assays relative to a “gold standard” measure of disease predisposition. In this commentary, we provide examples of some of the functional assays under development for several cancer predisposition genes (BRCA1, BRCA2, CDKN2A, and mismatch repair [MMR] genes MLH1, MSH2, MSH6, and PMS2) and present a detailed review of the issues associated with functional assay development. We conclude that validation is paramount for all assays that will be used for clinical interpretation of inherited variants of any gene, but note that in certain circumstances information derived from incompletely validated assays may be valuable for classification of variants for clinical purposes when used to supplement data derived from other sources. Hum Mutat 29(11), 1314–1326, 2008. © 2008 Wiley‐Liss, Inc.
Quality of life in Caribbean youth with diabetes.

BACKGROUND As the prevalence of youth onset diabetes in Jamaica increases, the impact of this disease on Health Related Quality of life (QOL) needs to be evaluated. METHODS Patients from two major referral hospitals had QOL assessed using a modified diabetes-39 questionnaire administered by a trained interviewer. Subscale scores were transformed to standard scale scores ranging from 0 (lowest impact) to 100 (highest impact) and the impact of socio-demographic factors, treatment and medical history on QOL examined. RESULTS Fifty-seven participants (21M, 36F, mean age 19.0 +/- 5.1 years, diabetes duration 2.7 +/- 2.2 years, 96% black) had QOL assessed. Anxiety and worry had the greatest impact (median score = 41) and sexual functioning, the lowest impact (median Score = 0) on QOL. Older age, later age of onset, longer diabetes duration, better glucose control and not using insulin were associated with a higher anxiety and worry score. Multiple regression analysis was conducted to determine which of these were associated with the anxiety and worry score after controlling for age. Anxiety and worry increased with age (regression coefficient (SE); 2.05[0.68]) and was higher in those on oral agents compared to those using insulin (-27.9 [10.9]). CONCLUSION Measures to address anxiety and worry in Jamaican youth with diabetes need to be implemented to minimize the impact the disease may have on their QOL.
Isolation and Characterization of Multiply Antibiotic-Resistant Clostridium perfringens Strains from Porcine Feces

Multiply antibiotic-resistant strains of Clostridium perfringens were isolated from porcine feces. Strains that were resistant to tetracycline, erythromycin, clindamycin, and lincomycin were isolated, but no penicillin- or chloramphenicol-resistant strains were obtained. Typical minimal inhibitory concentrations for resistant strains were 16 to 64 μg of tetracycline per ml, 64 to >128 μg of erythromycin per ml, ≥128 μg of lincomycin per ml, and 16 to 128 μg of clindamycin per ml. Resistance to erythromycin was always associated with resistance to lincomycin and clindamycin. Minimal inhibitory concentrations were determined for 258 strains from six farms that used antibiotics in their feeds and 240 strains from five farms that did not use antibiotics. The results show that 77.9 and 22.7% of the strains from the former farms were resistant to tetracycline and erythromycin-clindamycin-lincomycin, respectively. The comparable data from the latter farms were 25.0 and 0.8%, respectively. Agarose gel electrophoresis failed to reveal a plasmid band that was common to the resistant strains but absent in the susceptible strains. Attempts to transfer tetracycline, erythromycin, and clindamycin resistance from one strain, CW459, were not successful. Antibiotic-susceptible mutants were not isolated from this strain, despite the use of a variety of curing agents.
Making meaningful improvements to direct care worker training through informed policy: Understanding how care setting structure and culture matter

ABSTRACT Well-intentioned policy governing the training of direct care workers (DCWs) who serve older persons, in practice, may become merely a compliance issue for organizations rather than a meaningful way to improve quality of care. This study investigates the relationships between best practices in DCW training and the structure and culture of long term support service (LTSS) organizations. Using a mixed-methods approach to analyzing data from 328 licensed LTSS organizations in Pennsylvania, the findings suggest that public policy should address methods of training, not just content, and consider organizational variations in size, training evaluation practices, DCW integration, and DCW input into care planning. Effective training also incorporates support for organizations and supervisors as key aspects of DCWs’ learning and working environment.
Incidence and antimicrobial susceptibility of Porphyromonas gingivalis isolated from mixed endodontic infections.

AIM To investigate the prevalence of Porphyromonas gingivalis in root canals of infected teeth with periapical abscesses and to investigate the antimicrobial susceptibility of this species to some frequently prescribed antibiotics. METHODOLOGY Samples were obtained from 70 root canals of abscessed teeth. Microbial sampling, isolation and bacterial identification were accomplished using appropriate culture methods for anaerobic species. The antimicrobial susceptibility of the 20 strains of P. gingivalis isolated was determined by using the E-test. The antimicrobial agents tested were amoxicillin, amoxicillin + clavulanate, azythromycin, benzylpenicillin, cephaclor, clindamycin, erythromycin, metronidazole and tetracycline. RESULTS A total of 352 individual strains, belonging to 69 different species, were isolated. Eighty three percent of the strains were strict anaerobes and 47.5% of the isolated bacteria were Gram-negative. Porphyromonas gingivalis was found in 20 root canals and was most frequently found in symptomatic cases. Statistically, the presence of P. gingivalis was related to purulent exudates and pain on palpation (both P < 0.05). All P. gingivalis strains were sensitive to amoxicillin, amoxicillin + clavulanate, cephaclor, clindamycin, benzylpenicyllin, metronidazole and tetracycline. The lowest range of minimum inhibitory concentration (MIC) (0.026-0.125 microg mL(-1)) was observed against amoxicillin + clavulanate and clindamycin. The lowest MIC 90 was observed against clindamycin (0.064 microg mL(-1)). One strain was resistant to erythromycin and eight strains were resistant to azythromycin. CONCLUSION Porphyromonas gingivalis pathogen is isolated with frequency from root canals of infected teeth with periapical abscesses. Amoxicillin, as well as amoxicillin-clavulanic acid and benzylpenicillin were effective against P. gingivalis.
Outer membrane vesicles from group B Neisseria meningitidis Δgna33 mutant: Proteomic and immunological comparison with detergent‐derived outer membrane vesicles

We compared the proteome of detergent‐derived group B Neisseria meningitidis (MenB) outer membrane vesicles (DOMVs) with the proteome of outer membrane vesicles (m‐OMVs) spontaneously released into culture supernatant by MenB Δgna33, a mutant in which the gene coding for a lytic transglycosylase homologous to the E. coli MltA was deleted. In total, 138 proteins were identified in DOMVs by 1‐ and 2‐DE coupled with MS; 64% of these proteins belonged to the inner membrane and cytoplasmic compartments. By contrast, most of the 60 proteins of m‐OMVs were classified by PSORT as outer membrane proteins. When tested for their capacity to elicit bactericidal antibodies, m‐OMVs elicited a broad protective activity against a large panel of MenB strains. Therefore, the identification of mutations capable of conferring an OMV‐releasing phenotype in bacteria may represent an attractive approach to study bacterial membrane composition and organization, and to design new efficacious vaccine formulations.
A 140-mV Variation-Tolerant Deep Sub-Threshold SRAM in 65-nm CMOS

This paper presents a sub-threshold SRAM, which eliminates bitline (BL) leakage-induced read failures. The proposed architecture clamps the current ratio between differential BLs to a fixed value, thus permitting reliable ultra-low-voltage read-out. A de-multiplexed wordline interleaving scheme is presented to compensate for bitcell area overhead. The interleaving technique achieves 9% reduction in decoder area and 50% reduction in clock load within the decoder. A sense amplifier circuit with reduced sensitivity to process variations is proposed to further enhance the reliability of the differential read-out. Measurement results from a 1-kb SRAM, fabricated in an industrial 65-nm low-power CMOS process, show 13.1-kHz operation at 140 mV, with active read and leakage power figures of 30.5 and 28.1 nW, respectively.
Confirmatory Factor Analysis of Internet Use and Addiction

The ever-changing nature of the Internet continues to fuel questions as to its benefits and possible drawbacks. One issue that is particularly problematic is the validity of claims that the Internet is addictive. The present study used a confirmatory factor analysis (CFA) on a data set of 527 participants using the same survey developed by Pratarelli et al. (1) We propose a theoretical model of the relationship between (1) Internet addiction, (2) a sexual factor, and (3) an Internet use factor. These three factors were tested using two structural equation models generated on LISREL. The CFA revealed that the three factors are not orthogonal to each other, but instead are related to some degree as one would expect if a supposed Internet addicted individual exhibited behaviors related to each factor. We next tested whether the addiction was the causal factor leaving the sex and User factors as endogenous (i.e., resulting from the addiction). The second structural equation tested the contrasting possibility that sex and User related activities were exogenous to the addiction factor (i.e., the addiction resulted from these two activities). The analysis revealed that model 1, where the addiction is the causal factor behind the sex and User factors, had a stronger model fit index. On the basis of these current data it appears that Internet addiction may involve an addictive performance profile which in turn leads to excessive behaviors that involve (1) use of the Internet for sexual purposes and (2) its functional usefulness for a variety of professional and personal goals.
New oil-in-salt liquid-phase microextraction on permutite for the extraction and concentration of alkaloids in Coptis chinensis.

A novel oil-in-salt liquid-phase microextraction was developed and introduced for the extraction and concentration of the trace levels of active alkaloids in Coptis chinensis prior to being analyzed by high-performance liquid chromatography with ultraviolet detection. Also, the oil-in-salt extraction mechanism was analyzed, the enrichment factor and extraction recovery were redefined, and the proposed method was compared with other methods. In the approach, the mixed solvent of pentanol/octanol (6:4, v/v) and NaCl (20% w/v) are immobilized on the permutite surface in turn to form oil-in-salt double membranes, through which the target analytes can be molecularized though salting-out effect and be extracted by organic solvent. The main parameters affecting the approach were investigated and optimized. Under the optimized conditions, the enrichment factors of the analytes were 30-117, the linear ranges were 0.002-2 μg/mL for jatrorrhizine, coptisine, and palmatine, and 0.001-3 μg/mL for berberine (r2 ≥ 0.9923). The limits of detection were less than 1 ng/mL. Satisfactory recoveries (84.3%-120.3%) and precision (0.9%-7.5%) were also obtained. These results confirm that the approach is a simple and reliable sample pretreatment procedure and allows for the quantification of active alkaloids in C. chinensis at actual concentration levels.
Erythropoietin Promotes Bone Formation through EphrinB2/EphB4 Signaling

Recent studies have demonstrated that erythropoietin (EPO) has extensive nonhematopoietic biological functions. However, little is known about how EPO regulates bone formation, although several studies suggested that EPO can affect bone homeostasis. In this study, we investigated the effects of EPO on the communication between osteoclasts and osteoblasts through the ephrinB2/EphB4 signaling pathway. We found that EPO slightly promotes osteoblastic differentiation with the increased expression of EphB4 in ST2 cells. However, EPO increased the expression of Nfatc1 and ephrinB2 but decreased the expression of Mmp9 in RAW264.7 cells, resulting in an increase of ephrinB2-expressing osteoclasts and a decrease in resorption activity. The stimulation of ephrinB2/EphB4 signaling via ephrinB2-Fc significantly promoted EPO-mediated osteoblastic differentiation in ST2 cells. EphB4 knockdown through EphB4 shRNA inhibited EPO-mediated osteoblastic phenotypes. Furthermore, in vivo assays clearly demonstrated that EPO efficiently induces new bone formation in the alveolar bone regeneration model. Taken together, these results suggest that ephrinB2/EphB4 signaling may play an important role in EPO-mediated bone formation.
Oct‐4: Gatekeeper in the Beginnings of Mammalian Development

The Oct‐4 POU transcription factor is expressed in mouse totipotent embryonic stem and germ cells. Differentiation of totipotent cells to somatic lineages occurs at the blastocyst stage and during gastrulation, simultaneously with Oct‐4 downregulation. Stem cell lines derived from the inner cell mass and the epiblast of the mouse embryo express Oct‐4 only if undifferentiated. When embryonic stem cells are triggered to differentiate, Oct‐4 is downregulated thus providing a model for the early events linked to somatic differentiation in the developing embryo. In vivo mutagenesis has shown that loss of Oct‐4 at the blastocyst stage causes the cells of the inner cell mass to differentiate into trophectoderm cells. Recent experiments indicate that an Oct‐4 expression level of roughly 50%‐150% of the endogenous amount in embryonic stem cells is permissive for self‐renewal and maintenance of totipotency. However, upregulation above these levels causes stem cells to express genes involved in the lineage differentiation of primitive endoderm. These novel advances along with latest findings on Oct‐4‐associated factors, target genes, and dimerization ability, provide new insights into the understanding of the early steps regulating mammalian embryogenesis.
Sleep inertia: best time not to wake up?

Sleep inertia is a brief period of inferior task performance and/or disorientation immediately after sudden awakening from sleep. Normally sleep inertia lasts < 5 min and has no serious impact on conducting routine jobs. This preliminary study examined whether there are best and worst times to wake up stemming from circadian effects on sleep inertia. Since the process of falling asleep is strongly influenced by circadian time, the reverse process of awakening could be similarly affected. A group of nine subjects stayed awake for a 64-h continuous work period, except for 20-min sleep periods (naps) every 6 h. Another group of 10 subjects stayed awake for 64 h without any sleep. The differences between these two groups in performance degradation are expected to show sleep inertia on the background of sleep deprivation. Sleep inertia was measured with Baddeley's logical reasoning task, which started within 1 min of awakening and lasted for 5 min. There appeared to be no specific circadian time when sleep inertia is either maximal or minimal. An extreme form of sleep inertia was observed, when the process of waking up during the period of the circadian body temperature trough became so traumatic that it created "sleep (nap) aversion. " The findings lead to the conclusion that there are no advantages realized on sleep inertia by waking up from sleep at specific times of day.
Mathematical modeling for the prediction and optimization of laser hair removal

The study of hair removal is a slow, tedious process. Efficacy evaluations require test‐site observation for at least one complete hair cycle, a minimum of 6–8 months. In addition, tracking and counting individual hairs is extremely labor intensive. The objective of this study was to develop and evaluate a mathematical model for hair removal that could significantly speed the entire process.
THE IRON CAGE REVISITED:

Instead of examining why organizations are dissimilar, this study explores why organizations tend to be increasingly and inevitably homogenous in their forms and practices. Organizations in a similar line of work are structured into an organizational field by powerful forces that lead them to become similar. Rather than the causes of rationalization and bureaucratization suggested by Max Weber, including competition and the need for efficiency, institutional similarity is due to the structuration of organizational fields, a process caused largely by the state and the professions, which are the great rationalizers of the late 20th century. In highly structured organizational fields, rational efforts of individuals aggregately lead to structural, cultural, and output homogeneity. Homogenization is best captured by the concept of isomorphism, the process whereby one element in a population resembles others that confront the same environmental conditions. The two types of isomorphism are competitive and institutional. Three processes lead to organizational similarity: (1) coercive isomorphism stemming from political influence and the problem of legitimacy; (2) mimetic isomorphism resulting from uniform responses to uncertainty; and (3) normative isomorphism associated with professionalism. While these isomorphic processes improve organizational transactions, they do not necessarily increase internal efficiency. Twelve hypotheses are offered for further research about which organizational fields will be most homogenous. These hypotheses relate the impact of resource centralization and dependency, goal ambiguity and technical uncertainty, and professionalism and structuration on isomorphic change. Finally, useful implications of the study for theories of organizations and social change are offered. (TNM)