Miracle-dz/UniFAR-FTUs huggingface dataset:
Portuguese Web Accessibility Snapshot - Status of the Portuguese Websites Regarding Accessibility Levels

The Internet is extremely important for the publishing of information and for the interaction between the society elements. Due to this, it’s essential that the web presents itself accessible to all, including those with any kind of disability. An accessible web may help the handicapped citizens interact with the society in a more active way. This paper presents a study made with a universe of 777 Portuguese biggest enterprises, using a W3C referenced tool (SortSite), and presents some statistical results according to the Portuguese Classification of the Enterprises Activities. The achieved results present a serious troubled reality that’s preventing the disabled citizens from having the same access rights to the World Wide Web as the “nondisabled” citizens.
Adherence and invasion of mouse-adapted H pylori in different epithelial cell lines.

AIM To assess the adhesion and invasion abilities of different mouse adapted H pylori strains in different cell lines in vitro and investigate their effects on the virulence factors cagA and vacA. METHODS The adherence and invasion abilities of different H pylori strains in different epithelial cell lines were examined by the gentamycin protection assay. The null mutants of cagA and vacA were processed by direct PCR mutation method. The morphologic changes of different cell lines after H pylori attachment were examined by microscopy. RESULTS The densities of adherence to and invasion into cells in vitro were different from those in the mouse infection experiments. 88-3887 strain could invade and adhere to cells stronger than SS1 and X47. All tested strains had better adhering and invasive abilities in SCG-7901 cell. CagA and vacA minus mutants had the same invasion and adherent abilities as their wild types. In all strains and cell lines tested, only AGS cell had the significant hummingbird phenotype after inoculation with the 88-3887 wild-type. CONCLUSION Both the host cells and the bacteria play important parts in the invasion and adhesion abilities of H pylori. CagA and VacA are not related to the ability of invasion and adhesion of H pylori in different cell lines in vitro.
Speciation and Distribution of the Members of the Anopheles punctulatus (Diptera: Culicidae) Group in Papua New Guinea

Abstract Mosquito collections were made throughout the mainland of Papua New Guinea to identify the members of the Anopheles punctulatus group present and to determine their distribution. Identification was made using morphology, DNA hybridization, and polymerase chain reaction (PCR)-RFLP analysis. Nine members of the group were identified: An. farauti s.s. Laveran, An. farauti 2, An. koliensis Owen, and An. punctulatus Dönitz, were common and widespread; An. farauti 4 was restricted to the north of the central ranges where it was common; An. farauti 6 was found only in the highlands above 1,000 m; and An. farauti 3, An. sp. near punctulatus and An. clowi Rozeboom & Knight were uncommon and had restricted distributions. Identification of An. koliensis and An. punctulatus using proboscis morphology was found to be unreliable wherever An. farauti 4 occurred. The distribution and dispersal of the members of the An. punctulatus group is discussed in regard to climate, larval habitats, distance from the coast, elevation, and proximity to human habitation.
AAV-Tau Mediates Pyramidal Neurodegeneration by Cell-Cycle Re-Entry without Neurofibrillary Tangle Formation in Wild-Type Mice

In Alzheimer's disease tauopathy is considered secondary to amyloid, and the duality obscures their relation and the definition of their respective contributions. Transgenic mouse models do not resolve this problem conclusively, i.e. the relative hierarchy of amyloid and tau pathology depends on the actual model and the genes expressed or inactivated. Here, we approached the problem in non-transgenic models by intracerebral injection of adeno-associated viral vectors to express protein tau or amyloid precursor protein in the hippocampus in vivo. AAV-APP mutant caused neuronal accumulation of amyloid peptides, and eventually amyloid plaques at 6 months post-injection, but with only marginal hippocampal cell-death. In contrast, AAV-Tau, either wild-type or mutant P301L, provoked dramatic degeneration of pyramidal neurons in CA1/2 and cortex within weeks. Tau-mediated neurodegeneration proceeded without formation of large fibrillar tau-aggregates or tangles, but with increased expression of cell-cycle markers. We present novel AAV-based models, which demonstrate that protein tau mediates pyramidal neurodegeneration in vivo. The data firmly support the unifying hypothesis that post-mitotic neurons are forced to re-enter the cell-cycle in primary and secondary tauopathies, including Alzheimer's disease.
Efficacy and safety of antidepressant augmentation with lamotrigine in patients with treatment-resistant depression: a randomized, placebo-controlled, double-blind study.

OBJECTIVE This study reports a clinical trial evaluating lamotrigine safety and efficacy as an antidepressant augmentation agent in treatment-resistant depression, therefore adding more empirical evidence to the limited number of studies on the use of lamotrigine. METHOD A double-blind pilot study was conducted between March 2004 and January 2006 with 34 nonbi-polar, nonpsychotic patients who had DSM-IV major depressive disorder and were resistant to at least 2 anti-depressants. The subjects were taking antidepressant therapy and were randomly assigned to receive placebo or lamotrigine as an adjunct therapy for 8 weeks. They were evaluated on a biweekly basis in order to assess the efficacy and the safety of the drug. Efficacy was measured with the Montgomery-Asberg Depression Rating Scale (MADRS) and the Clinical Global Impressions (CGI) scale. Response was defined as a decrease of at least 50% from baseline on the MADRS and a final score ≤ 2 on the CGI. Safety was assessed by keeping record of treatment-emergent adverse events. RESULTS The results of the adjunct treatment with lamotrigine did not reveal a significant difference according to the MADRS (p = .45). No differences between the 2 treatment groups were revealed by the repeated-measures analysis of variance or by the analysis based on the CGI (p = .45). More than 50% of the patients had been treated with at least 3 different anti-depressants. The most frequent adverse events were nausea, rash, and dyspepsia in the lamotrigine group and dizziness and headache in the placebo group. CONCLUSIONS In this study, although it was safe, lamotrigine was not found to be an efficient augmentation agent in treatment-resistant depression. Small sample size, higher chronicity, and refractoriness may be related to treatment failure. TRIAL REGISTRATION clinicaltrials.gov Identifier: NCT00652171.
Aortic and Peripheral Blood Pressure During Isometric and Dynamic Exercise

The purpose of this study was to compare aortic blood pressure (AOR) to peripheral measurements by the Riva-Rocci/Korotkov (RRK) and Finapres continuous finger pressure (FIN) methods during dynamic and static exercise. A tip manometer was introduced in the ascending aorta after coronary angiography in 7 cardiac patients with good exercise capability. Static exercise was of moderate intensity and led to an increase of average diastolic and systolic AOR of 20 and 18 mmHg, respectively. The corresponding RKK values were 20 and 30 mmHg and the FIN values were 16 and 14 mmHg, respectively. In maximal cycle ergometry the discrepancies were larger, especially in the 4 subjects who reached 80% or more of predicted maximal work load. Diastolic and systolic increases in AOR in these 4 subjects were 12 and 38 mmHg, respectively. The RRK values were 17 and 76 mmHg. Increases in FIN values of 17 and 74 mmHg for diastolic and systolic measurements, respectively, were found. The peripheral FIN and RRK measurements give a systolic increase that is twice as large as that for AOR. It is concluded that RRK and FIN greatly overestimate the load to the cardiovascular system in dynamic exercise. When the cardiovascular load is estimated by the rate-pressure product, RRK produces an increase of 197%, FIN of 181%, while AOR gives an increase of only 133%. This suggests that the present criteria for blood pressure in exercise testing should be critically examined.
A Randomized, Double-Blind Clinical Trial of Vaginal Acidification versus Placebo for the Treatment of Symptomatic Bacterial Vaginosis

Background and Objectives Vaginal acidification has been suggested as a means of normalizing the vaginal flora. Goal The purpose of this study was to determine the effectiveness of an acetic acid-based vaginal gel in the treatment of bacterial vaginosis (BV). Study Design Forty-four patients with BV were enrolled in a randomized, double-blind clinic trial. Of these, 29 were evaluable. Patients were randomized to receive either 5 mL acetic acid gel (n = 14) or placebo gel (n = 15) intravaginally twice daily for 7 days. Clinical criteria and vaginal Gram stain scores were compared between the initial visit and at 2 weeks after initiating therapy. Results No significant differences were noted when comparing drug and placebo in terms of subjective or clinical improvement or improvement in Gram stain smears for bacterial vaginosis. Conclusion Vaginal acidification with an acetic acid gel formulated to pH 3.9 to 4.1 was ineffective therapy for bacterial vaginosis.
RELIABILITY ASSESSMENT OF BALLISTIC JUMP SQUATS AND BENCH THROWS

The purpose of this investigation was to determine the test-retest reliability and coefficient of variation of 2 novel physical performance tests. Ten healthy men (22.0 ± 3.0 years, 87.0 ± 8.0 kg, 20.0 ± 5.0% body fat) performed 30 continuous and dynamic jump squats (JS) and bench throws (BT) on 4 separate occasions. The movements were performed under loaded conditions utilizing 30% of subject's predetermined 1 repetition maximum in the back squat and bench press. Mean power (MP; W), peak power (PP; W), mean velocity (MV; m·s-1), peak velocity (PV; m·s-1), and total work (TW; J) were assessed using a ballistic measurement system (Innervations Inc., Muncie, IN). Data were analyzed using repeated measures analysis of variance with Duncan's post hoc test when mean differences were p ≤ 0.05. Intraclass correlation coefficient (ICC) and within-subject coefficient of variation (CV%) were also calculated. All values are presented as mean 6 SE. BT variables were statistically similar across the 4 sessions: MP (350.0 ± 13.9 W), PP (431.4 ± 18.5 W) MV (1.6 6 0.03 m·s-1), PV (2.0 ± 0.03 m·s-1), and TW (199.1 ± 7.2 J). For JS, session 3 PP (1,669.8 ± 111.2 W) was significantly greater vs. sessions 1, 2, and 4 (1,601.2 ± 58.4 W). Session 4 MP (1,403.2 ± 88.6 W) and MV (1.9 6 0.1 m·s-1) for JS were significantly lower during sessions 1, 2, and 3 (MP: 1,479.4.5 ± 44.8 W, MV: 2.0 ± 0.05 m·s-1). TW (834.7 ± 24.3 J) and PV (2.2 ± 0.04 m·s-1) were staistically similar during all sessions for JS. The CVs ranged from 3.0 to 7.6% for the BT and 3.2 to 5.7% for the JS. ICCs for MP, PP, MV, PV, and TW were 0.92, 0.95, 0.94, 0.91, and 0.95, respectively, during BT. ICCs during JS for MP, PP, MV, PV, and TW were 0.96, 0.98, 0.94, 0.94, and 0.89, respectively. The results of the current study support the use of a 30 continuous and dynamic BT protocol as a reliable upper-body physical performance test, which can be administered with minimal practice. Slightly greater variability for JS was observed, although the test had high reliability.
Effect of pulp and paper mill effluent (BKME) on physiological parameters of roach (Rutilus rutilus) infected by the digenean Rhipidocotyle fennica.

Physiological parameters were measured after experimental infection of roach (Rutilus rutilus L.) with Rhipidocotyle fennica Gibson, Valtonen et Taskinen, 1992 (Digenea) cercariae. The fish were caught from two lakes: a eutrophic bleached kraft mill effluent (BKME)-contaminated lake and an oligotrophic unpolluted lake. The intensity of infection was followed up to 10 days post infection (p.i.) and physiological parameters indicating non-specific stress responses and the condition of fish were examined simultaneously. The mean abundance, the number of parasites per fish, of R. fennica was significantly higher in the fish from the contaminated water during the first two days p.i. , probably reflecting the decreased resistance of these fish to infection. The decrease of leukocrit, as well as the increase of the activity of transaminases (GOT and GPT) in infected fish of both groups are suggestive of pathological processes caused by cercariae penetrating the fish. A significantly lower leukocrit value, as well as higher alkaline phosphatase activity and plasma chloride levels were noted in fish originating from the contaminated lake compared to those from the unpolluted lake. No significant differences were noted in haematocrit, plasma protein and calcium values between the fish from the uncontaminated and contaminated lakes, or between the infected and uninfected control fish.
Expression of divIB of Bacillus subtilis during vegetative growth

Expression of the division initiation gene, divIB, of Bacillus subtilis vegetative growth was examined. lacZ fusion studies and transcription start point mapping have established that a sigma A promoter proximal to divIB is utilized in vivo. The -10 region of this promoter, which is located 93 bp upstream of the start codon, has been defined precisely by site-directed mutagenesis that destroys the promoter. Examination of transcripts by Northern (RNA) blotting has shown that there are at least two transcripts for divIB. The established proximal promoter was found to give rise to a very minor transcript which could not be convincingly demonstrated in wild-type cells but which became apparent upon insertion of a plasmid into the chromosome just upstream of this promoter. The major transcript for divIB originated from a site several kb upstream of the gene and is probably the same as the long polycistronic message also traversing the murD-spoVE-murG genes that was identified previously by others (A.D. Henriques, H. de Lencastre, and P.J. Piggot, Biochimie 74:735-748, 1992). Transcription from the proximal promoter alone, in an upstream-deletion mutant strain, provided sufficient DivIB for normal growth and division as well as sporulation.
There is no evidence of an association in children and teenagers between the apolipoprotein E ɛ4 allele and post‐traumatic brain swelling

Traumatic brain injury (TBI) is an important cause of mortality and disability in children and teenagers. A particular feature of the neuropathology at post‐mortem is brain swelling. The cause of the swelling in some cases is not known, while in others it is associated with traumatic axonal injury or hypoxia. Apolipoprotein E (APOE) ɛ4 allele is known to be an important genetic determinant of outcome in children after TBI. We hypothesized a relationship between possession of APOEɛ4 and diffuse traumatic brain swelling. A total of 165 cases aged between 2 and 19 years were identified from the department's tissue archive. APOE genotype was determined by polymerase chain reaction (PCR) in 106 cases. Bilateral swelling was present in 44 cases (11 with APOEɛ4), unilateral swelling in 25 cases (7 with APOEɛ4) and in 36 cases (9 with APOEɛ4) there was no evidence of brain swelling. There was no significant relationship between possession of APOEɛ4 and the presence of cerebral swelling (χ2 = 0.09, df = 2, P = 0.96). The 95% confidence interval for difference in proportions with swelling in those with and without the APOE ɛ4 is −19% to 22%. Thus, a significant relationship was not found between diffuse brain swelling and possession of APOEɛ4, and in this cohort of patients there was an identifying cause of the brain swelling in all cases.
A benign helminth alters the host immune system and the gut microbiota in a rat model system

Helminths and bacteria are major players in the mammalian gut ecosystem and each influences the host immune system and health. Declines in helminth prevalence and bacterial diversity appear to play a role in the dramatic rise of immune mediated inflammatory diseases (IMIDs) in western populations. Helminths are potent modulators of immune system and their reintroduction is a promising therapeutic avenue for IMIDs. However, the introduction of helminths represents a disturbance for the host and it is important to understand the impact of helminth reintroduction on the host, including the immune system and gut microbiome. We tested the impact of a benign tapeworm, Hymenolepis diminuta, in a rat model system. We find that H. diminuta infection results in increased interleukin 10 gene expression in the beginning of the prepatent period, consistent with induction of a type 2 immune response. We also find induction of humoral immunity during the patent period, shown here by increased IgA in feces. Further, we see an immuno-modulatory effect in the small intestine and spleen in patent period, as measured by reductions in tissue immune cells. We observed shifts in microbiota community composition during the patent period (beta-diversity) in response to H. diminuta infection. However, these compositional changes appear to be minor; they occur within families and genera common to both treatment groups. There was no change in alpha diversity. Hymenolepis diminuta is a promising model for helminth therapy because it establishes long-term, stable colonization in rats and modulates the immune system without causing bacterial dysbiosis. These results suggest that the goal of engineering a therapeutic helminth that can safely manipulate the mammalian immune system without disrupting the rest of the gut ecosystem is in reach.
Evaluation of the DMS Rapid E system for identification of clinical isolates of the family Enterobacteriaceae

A total of 387 unique clinical isolates of the family Enterobacteriaceae were examined with the new DMS Rapid E gram-negative identification system (DMS Laboratories, Inc., Flemington, N.J.) and the API 20E procedure (Analytab Products, Plainview, N.Y.). Altogether, 376 strains (97.2%) were correctly identified to species level within 4 h with the DMS Rapid E system; 366 strains (94.6%) were correctly identified with the API 20E after overnight incubation.
Research in Clinical Laboratory Science: Professionals' Involvement

OBJECTIVES: To describe current qualitative and quantitative aspects of research engagement and other scholarly activities conducted by clinical laboratory science (CLS) professionals across a range of employment settings. DESIGN: A link to a 3-part online survey was sent by electronic mail to 7,572 members of the American Society for Clinical Laboratory Science and 500 program directors. SETTING: email message, on-line survey PARTICIPANTS: all ASCLS members and all directors of accredited clinical laboratory educational programs MAIN OUTCOME MEASURES: Quantitative and qualitative measures of professionals' engagement in research and other scholarly activities RESULTS: 556 of 7572 (7.3%) persons completed the survey. Thirty-two percent of survey respondents reported spending between 1 to >40 work hours per week conducting research with 68% of respondents not participating in research activities. Conducting research is an employment requirement for 18% of survey participants. Twenty-nine percent of respondents have published at least one research article, and 47% of respondents who conduct research have published studies in the journal Clinical Laboratory Science. More than 57% of respondents participate in non-research scholarly activities as part of their employment. CLS professionals who conduct research are more likely to do applied, clinical, or educational research than other types of research. Fifty-seven percent of respondents who conduct research lack external funding for their work. Ninety-three percent of total research dollars is obtained by respondents who hold the Ph.D. degree. The perception of the importance of conducting research varies by employment position. Barriers to participation in research include lack of inclusion of research in the job description, time constraints, inadequate research funding, limited opportunity, and lack of space and equipment. CONCLUSIONS: CLS professionals participate in research in limited numbers, and are more likely to engage in non-research types of scholarly activities. Numerous barriers are identified which impose limits to conducting research. Over half of CLS's research efforts lack external funding. Although there was broad representation among participants across educational levels, employment settings, and job positions, the number of survey respondents was limited. Possible directions for future research include conducting this survey using members of additional professional organizations. ABBREVIATIONS: CLS = clinical laboratory science; ASCLS = American Society for Clinical Laboratory Science; NAACLS = National Accrediting Agency for Clinical Laboratory Science
Towards Autonomous Cars: The Effect of Autonomy Levels on Acceptance and User Experience

Surveys [8] show that people generally have a positive attitude towards autonomous cars. However, these studies neglect that cars have different levels of autonomy and that User Acceptance (UA) and User Experience (UX) with autonomous systems differ with regard to the degree of system autonomy. The National Highway Traffic Safety Administration (NHTSA) defines five degrees of car autonomy which vary in the penetration of cars with Advanced Driver Assistance Systems (ADAS) and the extent to which a car is taken over by autonomous systems. Based on these levels, we conducted an online-questionnaire study (N = 336), in which we investigated how UA and UX factors, such as Perceived Ease of Use, Attitude Towards using the system, Perceived Behavioral Control, Behavioral Intention to use a system, Trust and Fun, differ with regard to the degree of autonomy in cars. We show that UA and UX are highest in levels of autonomy that already have been deployed in modern cars. More specifically, perceived control and fun decrease continuously with higher autonomy. Furthermore, our results indicate that pre-experience with ADAS and demographics, such as age and gender, have an influence on UA and UX.