Miracle-dz/UniFAR-FTUs huggingface dataset:
Construction and Characterization of a proU-gfp Transcriptional Fusion That Measures Water Availability in a Microbial Habitat

ABSTRACT We constructed and characterized a transcriptional fusion that measures the availability of water to a bacterial cell. This fusion between the proU promoter from Escherichia coli and the reporter gene gfp was introduced into strains of E. coli, Pantoea agglomerans, and Pseudomonas syringae. The proU-gfp fusion in these bacterial biosensor strains responded in a quantitative manner to water deprivation caused by the presence of NaCl, Na2SO4, KCl, or polyethylene glycol (molecular weight, 8000). The fusion was induced to a detectable level by NaCl concentrations of as low as 10 mM in all three bacterial species. Water deprivation induced proU-gfp expression in both planktonic and surface-associated cells; however, it induced a higher level of expression in the surface-associated cells. Following the introduction of P. agglomerans biosensor cells onto bean leaves, the cells detected a significant decrease in water availability within only 5 min. After 30 min, the populations were exposed, on average, to a water potential equivalent to that imposed by approximately 55 mM NaCl. These results demonstrate the effectiveness of a proU-gfp-based biosensor for evaluating water availability on leaves. Furthermore, the inducibility of proU-gfp in multiple bacterial species illustrates the potential for tailoring proU-gfp-based biosensors to specific habitats.
SERUM LIPIDS, POSTHEPARIN PLASMA LIPASE ACTIVITIES AND GLUCOSE TOLERANCE IN PATIENTS WITH PROLACTINOMA

Serum total cholesterol and triglyceride levels were determined in forty‐seven women with prolactinoma and in eighty‐four age‐ and weight‐matched control women. Oral glucose tolerance tests (OGTT) were performed and postheparin plasma lipoprotein lipase (LPL) and hepatic lipase (HL) activities were determined in twelve patients before and after transsphenoidal removal of the prolactinoma.
The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb

Microglia play important roles in perinatal neuro- and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication in CX3C receptor-1 deficient (Cx3cr1−/−) mice. Microglia depletion significantly lowered spine density in young (developing) but not mature adult-born-granule-cells (abGCs) in the olfactory bulb. Two-photon time-lapse imaging indicated that microglia depletion reduced spine formation and elimination. Functionally, odor-evoked responses of mitral cells, which are normally inhibited by abGCs, were increased in microglia-depleted mice. In Cx3cr1−/− mice, abGCs exhibited reduced spine density, dynamics and size, concomitantly with reduced contacts between Cx3cr1-deficient microglia and abGCs' dendritic shafts, along with increased proportion of microglia-contacted spines. Thus, during adult neurogenesis, microglia regulate the elimination (pruning), formation, and maintenance of synapses on newborn neurons, contributing to the functional integrity of the olfactory bulb circuitry.
Cherubism: panoramic and CT features in adults.

OBJECTIVES To describe the panoramic radiographic and CT features of cherubism in an unselected series of 15 adults. METHODS In this cross-sectional study, 15 individuals aged 29-84 years with familial non-syndromal molecularly confirmed cherubism were examined with panoramic radiography and CT. Bone abnormalities were analysed and described. RESULTS 11 (73%) of the 15 adults had mandibular abnormalities. These abnormalities ranged from subtly detectable to severe, and were less prevalent and expansive but could be rather similar to the characteristic image features in children. Unilocular radiolucencies were more common than multilocular radiolucencies, and a specific feature of these abnormalities was that they were exclusively found in the anterior mandible. CONCLUSIONS The radiographic and CT abnormalities of cherubism in adults were frequent and extremely heterogeneous, with some distinct features.
Pourquoi les enfants africains quittent-ils l’école? Un modèle hiérarchique multinomial des abandons dans l’éducation primaire au Sénégal

Dans cet article nous analysons les abandons dans l’education primaire au Senegal a l’aide de modeles hierarchiques generalises en utilisant des donnees issues d’une enquete longitudinale de 1996 a 2001 et d’une enquete retrospective de 2003 sur les menages des memes eleves. Les resultats font apparaitre la plus forte exposition au risque de decrochage scolaire des eleves issus de groupes exercant certaines activites professionnelles. Les resultats confirment aussi que les filles sont plus victimes de l’abandon scolaire que les garcons. L’existence dans la famille d’enfants frequentant le college ou le lycee exerce un effet favorable sur la retention des eleves a l’ecole. Les autres resultats confirment l’influence positive de la disponibilite des livres sur la retention des eleves et l’effet negatif qu’exerce un niveau d’echec scolaire eleve ou la rarete de l’offre educative dans la communaute. Enfin, les abandons precoces frappent davantage les filles que les garcons, mais l’existence d’une proportion importante de filles dans la premiere annee d’etudes reduit la probabilite d’abandonner. Les politiques educatives disposent ainsi de nombreux leviers pour reduire fortement les decrochages scolaires.
Revisiting the Warburg effect in cancer cells with proteomics. The emergence of new approaches to diagnosis, prognosis and therapy

Most cancer cells exhibit elevated levels of glycolysis and this metabolic pathway seems to be related to a greater glucose uptake. This phenomenon, known as the Warburg effect, is considered one of the most fundamental metabolic alterations during malignant transformation. Originally, Warburg hypothesised that the aerobic glycolysis of cancer cells could be just an aspect of a more complex metabolic adaptation. However, this intriguing discovery was partially misinterpreted and disregarded over time. In recent years, the peculiarities of cancer cell metabolism have been re‐evaluated in light of new metabolic data that seem to confirm and to widen the original concept of the Warburg effect. In fact, biochemical, molecular, and, above all, proteomic studies on the multifaceted roles of glycolytic enzymes in cancer cells in general, and in cancer stem cells in particular, seem to suggest more complex functional adaptations. These adaptations result in significantly altered protein expression patterns, and they have fundamental implications for diagnosis, prognosis and therapy. Revisiting the Warburg effect in cancer cells with a proteomic approach could deepen our knowledge of cancer cell metabolism and of cancer cell biology in general. Moreover, by identifying useful diagnostic, prognostic and therapeutic targets, it could significantly impact clinical practice.
Major depressive disorder in an adolescent with Turner syndrome: a case report

Abstract Turner syndrome (TS) is a chromosomal abnormality, of which the presence and impact of coexisting psychiatric morbidity has received little attention. The present report describes an adolescent with mosaic karyotype TS who had major depressive disorder with the predisposing cause of psychosocial burden, and relieved with the treatment of sertraline and complete remission with combined use of estradiol valerate. The report suggests us to pay more attention on the mood disorders in children with TS, especially in adolescents. For treatment aspect, medications for improving the puberty development and short stature should be added to in addition to antidepressants if they had mood disorders.
Predicting Lewy Body Pathology in a Community-Based Sample With Clinical Diagnosis of Alzheimer’s Disease

Accurate antemortem prediction of Lewy body pathology in patients with dementia is problematic. This study generates a model that better predicts Lewy body pathology in community-based patients with clinical Alzheimer’s disease. Lewy body pathology was detected in 80 of 152 participants (52.6%) with an initial diagnosis of probable Alzheimer’s disease. In a stepwise logistic regression model, female gender, lower education, being married, bradykinesia, hallucinations, and absence of irritability predicted the greatest likelihood of Lewy body pathology. The predictive model correctly diagnosed Lewy body pathology with an estimated sensitivity of 75%, specificity of 68%, and accuracy of 72%; the area under the receiver operating characteristic curve was 0.75. In a community-based autopsy sample, this predictive model confirmed parkinsonism and hallucinations as important predictors of Lewy body pathology in patients with clinical Alzheimer’s disease. The model also identified other demographic and clinical characteristics that might enhance the prediction of Lewy body pathology.
The Influence of Radiation Therapy on Dental Implantology

Patients with a history of head and neck cancer resection require extensive prosthodontic rehabilitation following cancer treatment. The oral anatomy drastically changes from ablative therapy, and the oral tissue response becomes altered as a consequence of radiation and chemotherapy. Successful restoration of oral function in this specific patient population was increasingly difficult before the widespread use of dental implants. Implant-borne prosthetics are now often used. However, surgical guidelines remain unclear with regard to oncology-related parameters. In this article, guidelines are introduced for implant therapy in the cancer patients according to radiation dosage and timing. Indications for hyperbaric oxygen treatment are highlighted along with risk assessment associated with implant placement. These guidelines are intended to augment knowledge obtained through oncology consultation; moreover, provide a rationale for implant therapy within the course of cancer treatment.
IN VITRO ANTIBACTERIAL ACTIVITY OF PEPPERMINT

Abstract Antibacterial activities of different forms of peppermint ( Mentha piperita ) viz., aqueous infusion, decoction, juice and essential oil were investigated against 100 isolates belonging to 11 different species of Gram –ve bacilli viz., Escherichia coli (30), Klebsiella pneumoniae (25), Pseudomonas aeruginosa (15), Salmonella typhi (5), S. paratyphi A (1), S. paratyphi B (1), Proteus mirabilis (10), P. vulgaris (2), Shigella dysentriae (5), Yersinia enterocolitica (1), and Enterobacter aerogenes (5). The screening was performed by standard disc diffusion method. Essential oil of peppermint exhibited highest antibacterial activity with 11.78 mm mean zone of inhibition. The juice of peppermint also possessed antibacterial activity with 10.41 mm mean zone of inhibition, while all isolates were totally resistant to aqueous infusion and decoction of peppermint. Introduction In recent years, multiple drug resistance has developed due to indiscriminate use of existing antimicrobial drugs in the treatment of infectious diseases (Service, 1995; Iwu
eview evelopment of circadian rhythms : Role of postnatal light environment

Mammals are born with an immature circadian system, which completes its development postnatally. Evidence suggests that the environment experienced by a newborn will impact and shape its development, which will have future consequences at the levels of circadian system function, circadian behaviour and physiology, and potentially, the animal’s long-term health and welfare. Here we review the various stages in postnatal development of the circadian system, and discuss the data available on the longterm effects of early environment, in particular light environment, on the animal’s brain, physiology and
Linguistic uses of segmental duration in English: acoustic and perceptual evidence.

The pattern of durations of individual phonetic segments and pauses conveys information about the linguistic content of an utterance. Acoustic measures of segmental timing have been used by many investigators to determine the variables that influence the durational structure of a sentence. The literature on segmental duration is reviewed and related to perceptual data on the discrimination of duration and to psychophysical data on the ability of listeners to make linguistic decisions on the basis of durational cues alone. We conclude that, in English, duration often serves as a primary perceptual cue in the distinctions between (1) inherently long verses short vowels, (2) voiced verses voiceless fricatives, (3) phrase‐final verses non‐final syllables, (4) voiced versus voiceless postvocalic consonants, as indicated by changes to the duration of the preceding vowel in phrase‐final positions, (5) stressed verses unstressed or reduced vowels, and (6) the presence or absence of emphasis.Subject Classification.
Molecular Outflows in Local ULIRGs: Energetics from Multitransition OH Analysis

We report on the energetics of molecular outflows in 14 local ultraluminous infrared galaxies (ULIRGs) that show unambiguous outflow signatures (P Cygni profiles or high-velocity absorption wings) in the far-infrared lines of OH measured with the Herschel/PACS spectrometer. All sample galaxies are gas-rich mergers at various stages of the merging process. Detection of both ground-state (at 119 and 79 μm) and one or more radiatively excited (at 65 and 84 μm) lines allows us to model the nuclear gas (≲300 pc) and the more extended components using spherically symmetric radiative transfer models. Reliable models and the corresponding energetics are found in 12 of the 14 sources. The highest molecular outflow velocities are found in buried sources, in which slower but massive expansion of the nuclear gas is also observed. With the exception of a few outliers, the outflows have momentum fluxes of (2–5) × LIR/c and mechanical luminosities of (0.1–0.3)% of LIR. The moderate momentum boosts in these sources (≲3) suggest that the outflows are mostly momentum driven by the combined effects of active galactic nuclei (AGNs) and nuclear starbursts, as a result of radiation pressure, winds, and supernova remnants. In some sources (∼20%), however, powerful (1010.5–11 L⊙) AGN feedback and (partially) energy-conserving phases are required, with momentum boosts in the range of 3–20. These outflows appear to be stochastic, strong AGN feedback events that occur throughout the merging process. In a few sources, the outflow activity in the innermost regions has subsided in the past ∼1 Myr. While OH traces the molecular outflows at subkiloparsec scales, comparison of the masses traced by OH with those previously inferred from tracers of more extended outflowing gas suggests that most mass is loaded (with loading factors of ) from the central galactic cores (a few × 100 pc), qualitatively consistent with an ongoing inside-out quenching of star formation. Outflow depletion timescales are <108 yr, shorter than the gas consumption timescales by factors of 1.1–15, and are anticorrelated with the AGN luminosity.
Health incongruence in later life: implications for subsequent well-being and health care.

OBJECTIVE The premise that pessimistic health appraisals compromise well-being whereas optimistic appraisals are compensatory was examined in a longitudinal study of 232 community-dwelling older adults (ages 79-98 years). DESIGN Subjective health (SH) appraisals were contrasted with objective health (OH) to identify realists, whose ratings were congruent (SH = OH), distinguishing them from health pessimists (SH < OH) and health optimists (SH > OH), whose ratings were incongruent. Analyses of covariance were used to examine group differences 2 years later on well-being and health care. MAIN OUTCOME MEASURES Outcome measures were psychological well-being (life satisfaction, positive and negative emotions), functional well-being (objective and perceived physical activity, activity restriction), and health care (health care management, hospital admissions, length of hospital stays). RESULTS Compared with realists, pessimists had significantly poorer outcomes and optimists had better outcomes. Because perceived control (PC) was weaker among pessimists and stronger among optimists, supplemental analysis determined whether PC differences explained these findings. When accounting for PC, many pessimism and optimism effects became nonsignificant, yet effects on functional well-being remained unchanged. CONCLUSION Findings have implications for older adults at risk of functional decline.
Two distinct signaling pathways in hair cycle induction: Stat3-dependent and -independent pathways.

The hair follicle is an epidermal derivative that undergoes cycles of growth, involution, and rest. The hair cycle has well-orchestrated kinetics regulated by interactions between mesenchymal and epithelial cells, although the intracellular signals remain unclear. We previously established keratinocyte-specific Stat3-disrupted mice, by which we demonstrated that signal transducer and activator of transcription 3 (Stat3) is required for wound healing and anagen progression in the hair cycle. Growth factor-dependent migration of Stat3-disrupted keratinocytes was severely impaired, suggesting that not only wound healing but also telogen-to-anagen progression required organized keratinocyte migration in response to mesenchymal stimuli. In the present study, to examine whether Stat3 activation in keratinocytes is a prerequisite for hair cycle progression, we applied methods for experimental anagen induction to Stat3-disrupted mice. It was demonstrated that anagen was successfully induced in Stat3-disrupted as well as wild-type mice by chemical or mechanical stimulation, i.e. , by topical application of phorbol 12-myristate 13-acetate (PMA) or by hair plucking, respectively. This result indicated that anagen in these methods occurred in the absence of Stat3. Furthermore, PMA stimulated the migration of Stat3-disrupted keratinocytes in vitro, supporting a hypothesis that the protein kinase C (PKC) and Stat3 pathways occur independently in the postnatal anagen induction. Both Stat3-dependent and -independent migration of keratinocytes was inhibited by a phosphoinositide 3-kinase (PI3K) inhibitor, wortmannin. Therefore, we infer that entry into anagen is mediated by at least two distinct signaling pathways: Stat3-dependent pathway for spontaneous hair cycling and Stat3-independent (probably PKC-dependent) pathway for exogenously induced hair cycling, whereas both pathways require PI3K activation.
Cross-Neutralizing Antibody Profile of Chinese HIV-1-Infected Individuals and the Viral Envelope Features From Elite Neutralizers

Objective:Knowledge about the profile of neutralization responses and the viral envelope features of HIV-1-infected individuals in China may provide new insights for vaccine design against local viruses. Methods:Eight hundred sixty plasma samples from antiretroviral treatment-naive HIV-1-infected individuals in Xinjiang province (611) and Guangxi province (249), who had acquired infection over 3 years through intravenous drug use or sexual contact, were examined for their ability to neutralize diverse envelope-pseudoviruses of 5 subtypes. The sequence features of the envelopes from elite neutralizers were analyzed. Results:From Xinjiang and Guangxi, 29.1% and 5.2% of plasmas displayed intrasubtype cross neutralization against subtype B and subtype C, respectively. From Xinjiang, 10.6% of the plasmas displayed broad neutralization against the 3 subtypes, B, C, and CRF01_AE; whereas only 2.4% from Guangxi displayed broad neutralization. Envelopes from 6 elite neutralizers were obtained by single-genome amplification. The variations of their envelopes including the lengths, glycans, and net charges in V1, V2, and V4 regions were compared with those from CRF07_BC env sequences from the HIV Sequence Database. The Envs from 3 elite neutralizers displayed the sensitivities to the monoclonal broadly neutralizing antibodies such as PG9, PG16, and 4E10. Some unique characteristics of the envelope glycoproteins from the Chinese elite neutralizers were found. Conclusions:The neutralization profile of HIV-1-infected individuals in the 2 regions of China, where the HIV-1 subtypes are the representative in China, and the unique characteristics of the envelope glycoproteins from the Chinese elite neutralizers provide useful information for viral infection prevention and an insight for vaccine design against locally epidemic viruses.
Optical interconnects at the top of the rack for energy-efficient data centers

The growing popularity of cloud and multimedia services is dramatically increasing the traffic volume that each data center needs to handle. This is driving the demand for highly scalable, flexible, and energy-efficient networks inside data centers, in particular for the edge tier, which requires a large number of interconnects and consumes the dominant part of the overall power. Optical fiber communication is widely recognized as the highest energy- and cost-efficient technique to offer ultra-large capacity for telecommunication networks. It has also been considered as a promising transmission technology for future data center applications. Taking into account the characteristics of the traffic generated by the servers, such as locality, multicast, dynamicity, and burstiness, the emphasis of the research on data center networks has to be put on architectures that leverage optical transport to the greatest possible extent. However, no feasible solution based on optical switching is available so far for handling the data center traffic at the edge tier. Therefore, apart from conventional optical switching, we investigate a completely different paradigm, passive optical interconnects, and aim to explore the possibility for optical interconnects at the top of the rack. In this article, we present three major types of passive optical interconnects and carry out a performance assessment with respect to the ability to host data center traffic, scalability, optical power budget, complexity of the required interface, cost, and energy consumption. Our results have verified that the investigated passive optical interconnects can achieve a significant reduction of power consumption and maintain cost at a similar level compared to its electronic counterpart. Furthermore, several research directions on passive optical interconnects have been pointed out for future green data centers.
The Long Term Recovery Curve in Adults after Median or Ulnar Nerve Repair: A Reference Interval

This study presents a predicted five-year reference interval for the outcome following repair of the median or ulnar nerve in adults. Forty-four patients were examined with the use of a recently introduced model instrument for documentation after nerve repair that includes “sensory”, “motor”, and “pain/discomfort” outcomes which together constitute a summarized “total score”. Analysis of the “total score” showed that follow-up time and age significantly influence the outcome. There were obvious inferior “motor” results after ulnar nerve injury, but these did not significantly influence the “total score”. Significant improvements in the “total score” were seen throughout the follow-up period.