Miracle-dz/UniFAR-FTUs huggingface dataset:
P 2 X and P 2 Y purinergic receptors on human intestinal epithelial carcinoma cells : effects of extracellular nucleotides on apoptosis and cell proliferation

Robson Coutinho-Silva, Lynn Stahl, Kwok-Kuen Cheung, Nathalia Enes de Campos, Carolina de Oliveira Souza, David M. Ojcius, and Geoffrey Burnstock Autonomic Neuroscience Institute, Royal Free and University College Medical School, London, United Kingdom; Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Université Paris 7, Institut Jacques Monod, Paris cedex 5, France; and School of Natural Sciences, University of California, Merced, California
Association of the XBP1−116C/G polymorphism with schizophrenia in the Japanese population

Abstract Schizophrenia and bipolar disorder share some clinical features and linkage studies have shown that several loci are common. Recently, the authors found that the −116C→G substitution in the promotor region of XBP1, a pivotal gene in endoplasmic reticulum (ER) stress response, causes the impairment of ER stress response, and that the −116C/C genotype is a protective factor; in other words the presence of the G allele increases the risk for bipolar disorder. The gene is located on 22q12.1, which is also linked with schizophrenia. The polymorphisms were investigated in 234 schizophrenic patients as compared with controls. Significant difference of genotype distribution was observed, which suggested that the −116C/C genotype is a protective factor for both of the major mental disorders.
It’s a Goal!: basing a community psychiatric nursing service in a local football stadium

This paper describes the development of a community mental health project in a local football stadium. Funded for three years by the Laureus Foundation’s ‘Sport for Good’ initiative, the project provides mental health promotion and mental health awareness input targeted initially at young men, a group who are often very difficult to engage in this type work. Using group interventions and utilising football as a metaphor, the project helps young men address issues around depression, self-esteem and inclusion, and addresses the subject of suicide which remains the second biggest cause of death in young men in Britain. The paper describes the development of the project, the structure of the groups and the evaluation of the first two groups to complete the process. The work takes place in the Moss Rose stadium, home of Macclesfield Town, a team in the English Football League.
Proximal Humeral Reconstruction After Excision of a Primary Sarcoma

From 1978 to 1991, 19 patients underwent wide resection of the proximal humerus for a primary bone tumor. Reconstructions included intercalary autoclaved autograft and Neer prosthesis composites in 11 patients, intercalary allograft and Neer prosthesis composites in 4 patients, and a Neer prosthesis with methylmethacrylate in 4 patients. All patients were observed for at least 2 years or until death. The functional results and complications of the 19 procedures were evaluated. Function was rated as excellent for 17 patients and good for 2 patients. There were no failures of fixation. Local recurrence occurred in 2 patients. There were 2 cases of recurrent anterior subluxation and 1 of dislocation. Two superficial wound infections were treated successfully with local debridement and antibiotics. The authors conclude that autoclaved autograft or allograft when combined with a long-stem cemented Neer prosthesis offers an excellent composite for proximal humeral reconstruction.
« Les groupes d'enfants de parents séparés et la participation des parents »

Ce texte compare cinq formules de groupe d'enfants du primaire ayant vecu la separation de leurs parents (trois groupes americains et deux groupes quebecois) et presente les resultats de l'evaluation de ces programmes. Une attention particuliere est accordee au degre de participation des parents et aux consequences possibles de cette participation pour les resultats de l'intervention. Ce texte discute egalement de l'apport des groupes d'enfants dans le domaine de la prevention et de la promotion en sante mentale, tout en les situant dans une perspective ecologique.
Blood pressure reactivity to psychological stress: a new risk factor for coronary disease?

The report by Matthews et al1 in this issue of Hypertension that increased systolic blood pressure reactivity while playing a video game predicts increased incidence of having some coronary calcification 13 years later is consistent with prior research they cite showing associations between cardiovascular reactivity to acute stress and various indices of atherosclerotic disease in human and animal studies. What sets this report apart from the prior research, however, is (1) the prediction of increased coronary artery calcification by blood pressure (BP) reactivity in a large, diverse sample of healthy young adults; (2) the demonstration that this prediction is independent of resting blood pressure and other established coronary heart disease (CHD) risk factors measured at the same time; and (3) the demonstration that the prediction is not mediated by interim development of hypertension or the metabolic syndrome. The authors are appropriately cautious in noting that final appraisal of the full implications of their findings will depend on results obtained when “BP reactivity protocols are added to future epidemiological protocols. ” As one who has previously joined with other behavioral and social science advocates in what seemed, in many cases, to be an uphill battle to convince those in charge of designing large-scale …
Residual Julia Sets of Meromorphic Functions

In this paper, we study the residual Julia sets of meromorphic functions. In fact, we prove that if a meromorphic function $f$ belongs to the class ${\bf S}$ and its Julia set is locally connected, then the residual Julia set of $f$ is empty if and only if its Fatou set $F(f)$ has a completely invariant component or consists of only two components. We also show that if $f$ is a meromorphic function which is not of the form $\alpha + (z\,-\,\alpha)^{-k}e^{g(z)}$, where $k$ is a natural number, $\alpha$ is a complex number and $g$ is an entire function, then $f$ has buried components provided that $f$ has no completely invariant components and its Julia set $J(f)$ is disconnected. Moreover, if $F(f)$ has an infinitely connected component, then the singleton buried components are dense in $J(f)$. This generalizes a result of Baker and Domínguez. Finally, we give some examples of meromorphic functions with buried points but without any buried components.
Predicting language outcome in infants with autism and pervasive developmental disorder.

BACKGROUND To examine longitudinal associations between diagnosis, joint attention, play and imitation abilities and language outcome in infants with autism and pervasive developmental disorder. METHODS AND PROCEDURES Experimental measures of joint attention, play and imitation were conducted with a sample of infants with autism spectrum disorder at age 20 months. Language outcome was assessed at age 42 months. A within-group longitudinal correlational design was adopted. OUTCOMES AND RESULTS Language at 42 months was higher for children with a diagnosis of pervasive developmental disorder than for children with a diagnosis of autism. Language at follow-up was also positively associated with performance on experimental measures of joint attention and imitation, but not with performance on experimental measures of play and 'goal detection' at 20 months, nor with a non-verbal intelligence quotient, although these associations were not examined independent of diagnosis. However, floor effects on the measure of play at 20 months and the small sample size limit the conclusions that can be drawn. CONCLUSIONS Individual differences in infant social-communication abilities as well as diagnosis may predict language outcome in preschoolers with autism spectrum disorders. Attention should be directed at assessing these skills in 2- and 3-year-old children referred for a diagnosis of autism spectrum disorder. Imitation and joint attention abilities may be important targets for early intervention.
Combined infrared light and bipolar radiofrequency for skin tightening in asians

As the demand for non‐invasive procedures for skin tightening is increasing, combined optical and radiofrequency (RF) devices have recently emerged. The purpose of this study is to evaluate the safety and efficacy of a device that combined broadband infrared (IR) light (700–2000 nm) and bipolar RF (electro‐optical synergy [ELOS]) for non‐ablative treatment of facial laxity.
Evaluation of Methods for Storage of Marine Macroorganisms with Optimal Recovery of Bacteria

ABSTRACT Marine macroorganisms are a potential source for new bioactive substances. In many cases marine microorganisms—especially bacteria—associated with these macroorganisms are actually producing the bioactive substances. One often is not able to immediately isolate microorganisms from collected macroorganismic materials; we therefore evaluated different methods for storage of such material, e.g., on board research vessels. These methods were the following: storage of macerates in sintered glass beads and 5% trehalose at −20°C (SGT method); storage of sections in 5% dimethyl sulfoxide at −70°C (SD method); storage of macerates at −20°C using the commercial ROTI-STORE system (RS method); storage of macerates at −20°C in 50% glycerol (GC method); and storage of macerates covered by mineral oil at 4°C (MO method). The SGT and SD methods resulted in numbers of and especially diversity of recoverable bacteria that were higher than for the other methods. Data for the RS method indicated its potential usefulness, too. The MO method resulted in growth during storage, thereby enriching a few selected microorganisms; the GC method resulted in a survival and diversity of recovered bacteria that was too low.
Differential Regulation of Iron- and Manganese-Specific MtsABC and Heme-Specific HtsABC Transporters by the Metalloregulator MtsR of Group A Streptococcus

ABSTRACT The genome of the human pathogen group A Streptococcus (GAS) encodes the transporters MtsABC, FtsABCD, and HtsABC to take up ferric and manganese ions, ferric ferrichrome, and heme, respectively. The GAS genome also encodes two metalloregulators PerR and MtsR. To understand the regulation of the expression of these transporters, the mtsR and perR deletion mutants of a GAS serotype M1 strain were generated, and the effects of the deletions and Fe3+, Mn2+, and Zn2+ on the expression of mtsA, htsA, and ftsB were examined. Mn2+ dramatically depresses mtsA transcription and levels of the MtsA protein but does not downregulate the expression of htsA and ftsB. Fe3+ decreases the expression of mtsA and htsA but has no effect on ftsB expression. Zn2+ has no effect on the expression of all three genes. The deletion of mtsR abolishes the Mn2+- and Fe3+-induced depression of mtsA expression and the Fe3+-dependent decrease in htsA expression. The deletion of mtsR does not significantly alter GAS virulence in a mouse model of subcutaneous infection. The deletion of perR does not affect the expression of the genes in response to the metal ions. MtsR binds to the mts promoter region in the presence of Mn2+ or Fe2+. The results indicate that MtsR differentially regulates the expression of mtsABC and htsABC.
Plasma corticosterone responses to handling in Japanese quail selected for low or high plasma corticosterone responses to brief restraint

1. Plasma corticosterone responses to handling in Japanese quail selected for low or high corticosterone responses to brief mechanical restraint (low and high stress lines) were measured in this study. 2. Initial corticosterone concentrations did not differ between the two lines. Corticosterone concentrations increased 15 min after handling began in quail handled for 5 or 15 min, with greater increases in quail from the high stress than the low stress line. Integrated corticosterone responses were higher in high stress than low stress quail, although the difference between the lines was not significant in quail handled for 5 min. 3. Patterns of plasma corticosterone after 15 min differed between quail handled for 5 or 15 min. Corticosterone increased further or did not change from 15 to 30 min then decreased from 30 to 60 min in quail handled for 5 min, whereas corticosterone decreased from 15 to 30 min then did not change in quail handled for 15 min. Corticosterone concentrations at 60 min remained elevated above initial concentrations, and were similar in quail handled for 5 or 15 min. Corticosterone concentrations at 15 min were more than twice as high in quail handled for 15 min compared with quail handled for 5 min. 4. Although the lines of quail have been selected on the magnitude of corticosterone responses, there was still marked variation in responses between birds within each line. Some quail in the high stress line showed little or no corticosterone response to handling, whilst large responses were seen in some birds of the low stress line. The lines of quail differ in characteristics other than corticosterone responses, including fear behaviour and reproductive development, so the lines provide valuable opportunities to investigate the potential significance of differences between individual birds in their corticosterone responses.
Identification of del(6)(q21q25) as a recurring chromosomal abnormality in putative NK cell lymphoma/leukaemia

The putative natural killer (NK) cell lymphomas/leukaemias are a group of recently characterized haematolymphoid malignancies sharing an immunophenotype of CD3/Leu4− CD3ɛ+ CD56+, and a genotype of germline T‐cell receptor genes. They frequently present in extranodal sites and exhibit a highly aggressive clinical course. Information on the cytogenetic or molecular events leading to the tumourigenesis in this group of tumours is very scarce. In this study we analysed the cytogenetic findings of seven patients with CD56‐positive putative NK cell lymphoma/leukaemia. Three cases, including one nasal, one extranasal and one leukaemic form, showed a common region of deletion at 6q21‐q25, suggesting that this may be a non‐random chromosomal aberration.
Genomic strategy for targeting therapy in castration-resistant prostate cancer.

PURPOSE Despite treatments which lower circulating androgens, advanced prostate cancers often maintain androgen receptor (AR) signaling. The variable response to secondary hormonal manipulations in men with castrate-resistant prostate cancer (CRPC) creates a compelling need for strategies to individualize therapy based on the molecular features of each patient's tumor. METHODS A transcription-based AR activity signature was developed from an androgen-sensitive prostate cancer cell (LNCaP) and tested on independent data sets of prostate cancer cell lines and human tumors to assess its precision and accuracy in detecting AR activity. The AR signature was applied to multiple sets of prostate specimens to determine how AR activity changes with hormone therapy and progression and oncogenic pathway analysis was used to identify biologic pathways correlating with AR activity. RESULTS A robust AR signature accurately predicts AR activity in multiple prostate cancer cell lines, has minimal variation between replicate samples, and accurately reflects an individual's hormone status and intraprostatic dihydrotestosterone levels. The AR signature finds AR activity to be high in local, untreated prostate tumors and decreased in prostate tissue after neoadjuvant hormone therapy and in CRPC. Heterogeneity of AR activity exists along the spectrum of prostate cancer progression and decreasing predicted AR activity correlates with increasing predicted Src activity and sensitivity to dasatinib (Src-targeting kinase inhibitor). CONCLUSION A transcription-based AR signature can detect AR activity within individual prostate cancer specimens and has the potential to help individualize and improve care for patients with CRPC.
Occurrence of bifidobacteria in faeces of calves fed milk or a combined diet

The development of faecal bacteria composition in calves fed milk or a combined diet was investigated from 4 to 21 days of age. On day 7, bifidobacteria in faeces of milk-fed calves already increased from about 7.6 to 9.2 log CFU/g and did not change until the end of the study, whereas in calves fed the combined diet bifidobacteria only moderately increased to 7.9 log CFU/g and decreased slowly until day 21. The counts of bifidobacteria in calves on a combined diet were significantly (p < 0.01) lower compared to those in milk-fed calves. Bifidobacterial counts determined by cultivation or by fluorescence in situ hybridisation (FISH) did not differ significantly. Our results showed that the occurrence of bifidobacteria in calf faeces is highly dependent on the diet composition. Faecal bacteria flora of calves fed exclusively by milk is rich in bifidobacteria, but in calves on a combined diet coliforms dominated.
Partial characterization of radiolabelled antigens from adult Schistosoma haematobium.

Previously, Bolton-Hunter labeled adult S. mansoni proteins were fractionated by SDSPAGE to determine molecular weights and immunoprecipitated using human infection sera to detect cross-reactivity (Hayunga et al., 1979, J. Parasitol. 65: 488-496; 497-506). These observations are now extended to S. haematobium. In order to provide a direct comparison, the conditions of radiolabeling, detergent extraction and molecular weight determination were kept identical to those of the previous study. Cercariae from an Egyptian strain of S. haematobium in naturally infected Bulinus truncatus were used to infect golden hamsters in the laboratory. (We are grateful to Drs. P. LoVerde, K. Murrell, and V. Schinski for collecting infected snails in the field, and to Miss K. Groover, Mr. D. Olson, and K. Holman for maintaining the life cycle in the laboratory.) Approximately 20 to 30 adult worms, obtained by portal perfusion of 12-wk infected hamsters, were radiolabeled with 0.5 mCi 125I-Bolton-Hunter reagent as described previously (Hayunga et al., loc. cit.); the worms were motile and appeared undamaged after this procedure. Tegumental proteins were solubilized in 0.5% NP-40 for 15 min at 37 C, and the lysate run directly on SDS gels or immunoprecipitated as follows: Aliquots (approx. 100,000 c.p.m.) of labeled lysate were incubated overnight at 4 C with 25 ,ul serum, followed by incubation for 2 hr with 500 ,ul of a 10% w/v suspension of staphylococcal protein A (Pansorbin? , Calbiochem); after washing 3 times with BBS, samples were eluted with 4% SDS-6 M urea and fractionated by SDS-PAGE on 10% tube gels. Serum from patients infected by either S. haematobium or S. mansoni was provided by Dr. E. Higashi, NAMRU-3, Cairo; rabbit serum was prepared by subcutaneous inoculation of a freeze-thaw preparation of adult S. mansoni. Fractionation of radioiodinated S. haematobium proteins on SDS gels revealed heavily labeled peaks of >100,000, 60,000, 30,000, and 13,000 mol. wt. , and minor peaks of 85,000, 43,000, 36,000, 33,000, 25,000, 23,000, and 18,000 mol. wt. (Fig. 1A). Label also occurred in two peaks, presumed to contain lipid material, that migrated with and slightly ahead of the dye front. When samples were electrophoresed under reducing conditions using dithiothreitol, the large (>100,000) molecular weight peak dissociated but no additional peaks were found (Fig. 1B). In order to detect cross-reactivity, the labeled lysate from
The Vision of Autonomic Computing

A 2001 IBM manifesto observed that a looming software complexity crisis -caused by applications and environments that number into the tens of millions of lines of code - threatened to halt progress in computing. The manifesto noted the almost impossible difficulty of managing current and planned computing systems, which require integrating several heterogeneous environments into corporate-wide computing systems that extend into the Internet. Autonomic computing, perhaps the most attractive approach to solving this problem, creates systems that can manage themselves when given high-level objectives from administrators. Systems manage themselves according to an administrator's goals. New components integrate as effortlessly as a new cell establishes itself in the human body. These ideas are not science fiction, but elements of the grand challenge to create self-managing computing systems.
The Comparative Cytotoxic Effects of Different Local Anesthetics on a Human Neuroblastoma Cell Line

BACKGROUND:Although local anesthetics (LAs) are generally accepted as being safe, incidental neuronal damage has been reported for all LAs in humans. Therefore, in this study, we compared the dose corresponding to 50% cell lethality (LD50) of articaine, lidocaine, mepivacaine, bupivacaine, prilocaine, and ropivacaine in human neuroblastoma cells. METHODS:Undifferentiated SH-SY5Y cells were exposed for 20 minutes to different concentrations of each LA. Metabolic activity of viable cells was assessed by a cell viability test with a tetrazolium dye (WST-1) followed by optical density quantification. LD50 was determined by extrapolation of curve response. RESULTS:As expected, all LAs induced cell death in a concentration-dependent manner. The bupivacaine, lidocaine, prilocaine, mepivacaine, articaine, and ropivacaine LD50 were 0.95 ± 0.08, 3.35 ± 0.33, 4.32 ± 0.39, 4.84 ± 1.28, 8.98 ± 2.07, and 13.43 ± 0.61 mM, respectively, after 20 minutes of incubation on SH-SY5Y cells. Ropivacaine LD50 was significantly different from the bupivacaine, lidocaine, mepivacaine, and prilocaine LD50 (all P ⩽ 0.009). No significant difference was obtained between ropivacaine and articaine LD50 and between prilocaine, lidocaine, and mepivacaine LD50. Articaine LD50 was significantly different from lidocaine LD50 (P = 0.03). Bupivacaine LD50 was significantly lower compared with all LAs (all P ⩽ 0.003). CONCLUSIONS:LA neurotoxicity was tested in a validated in vitro model SH-SY5Y, a human neuroblastoma cell line. Three groups of LAs were identified in terms of toxicity: (1) the less (ropivacaine, articaine); (2) medium (mepivacaine, prilocaine, lidocaine); and (3) the high (bupivacaine). Among dental anesthetics, articaine is the least neurotoxic in SH-SY5Y cells.