Miracle-dz/UniFAR-FTUs huggingface dataset:
Twenty‐four hour kinetics of hepatitis C virus and antiviral effect of alpha‐interferon

Numerous studies reported that patients infected by the genotype 1 of hepatitis C virus (HCV) and/or with a high baseline viral load responded poorly to antiviral therapy. Study of viral kinetics has provided clues to the understanding of non‐response to alpha‐interferon (IFN‐α)‐based therapy. The objective of this study was to clarify the influence of viral factors such as the genotype and baseline viral load on HCV resistance to treatment through the study of their impact on the first phase of viral decline. HCV RNA levels were determined frequently following the administration of 3 million units of IFN‐α in 22 chronic HCV carriers. The evolution of HCV RNA level over 24 hr was different in genotype 1‐infected patients, compared to that in patients infected by other genotypes. The viral load decline at 24 hr was lower in patients with genotype 1. Patients with a high baseline viral load exhibited a viral dynamics different from patients with a lower level of viremia; the extent of the first phase was also lower in these patients. Non‐responder patients had a slower viral decay on day 1 of therapy than patients who cleared the virus under treatment. In conclusion, 24 hr HCV dynamics is regulated by genotype and baseline viral load. Genotype 1 strains and those that produce high viral loads are the most resistant to the antiviral action of IFN‐α. Resistant HCV strains could be distinguished from sensitive viruses as early as a few hours after the beginning of the treatment. J. Med. Virol. 78:365–371, 2006. © 2006 Wiley‐Liss, Inc.
Emblica officinalis Corrects Functional, Biochemical and Molecular Deficits in Experimental Diabetic Neuropathy by Targeting the Oxido‐nitrosative Stress Mediated Inflammatory Cascade

Diabetic neuropathy is one of the most common microvascular complications of diabetes mellitus which affects more than 50% of diabetic patients. Diabetic neuropathic pain is amongst the most difficult types of pain to treat mainly due to the lack of understanding of its etiology and inadequate relief with available drug therapy. The present study targeted oxidative stress mediated nerve damage in diabetic rats using an aqueous extract of Emblica officinalis, a potent natural antioxidant. Diabetic rats exhibited significantly decreased tail‐flick latency in the tail‐immersion test (thermal hyperalgesia) and decreased paw withdrawal threshold in both Randall‐Selitto (mechanical hyperalgesia) and von‐Frey hair test (mechanical allodynia). A decrease in the nociceptive threshold was accompanied by significantly increased oxidative stress, nitrite levels and cytokines (TNF‐α, IL‐1β and TGF‐β1) both in the serum and sciatic nerve of diabetic rats. Treatment with the Emblica officinalis aqueous extract (250, 500 and 1000 mg/kg/day) significantly attenuated all the behavioral, biochemical and molecular alterations in a dose‐dependent manner. The major finding of the study is that insulin alone corrected the hyperglycemia and partially reversed the pain response in diabetic rats. However, combination with Emblica officinalis extract not only attenuated the diabetic condition but also reversed neuropathic pain through modulation of oxidative–nitrosative stress in diabetic rats. Copyright © 2011 John Wiley & Sons, Ltd.
The Economics of Algorithmic Trading

Financial markets have undergone a dramatic technological transformation. Electronic and centralized limit order books dominate the organized securities exchange landscape. Banks and fund companies have invested in trading technology to improve their trade generation and monitoring processes. The automation of these processes is typically called Algorithmic Trading. This work presents one of the first studies of algorithmic trading and its impact on information and liquidity in equity markets.
Modeling of human immunodeficiency virus modes of transmission in Iran.

BACKGROUND Main technique to control acquired immunodeficiency syndrome (HIV) infection is the effective preventive programs among high-risk groups. Modeling is one of the effective methods where there is inadequate data. We used the modes of transmission (MOT) model to predict the transmission of HIV infection in Iran. METHODS We systematically searched published and grey literature to find values for the input parameters of MOT in 2010. The data were discussed by experts before being fed into the model. Using the Monte Carlo simulation, we computed the 95% confidence interval (CI) for the outputs of the MOT. RESULTS The MOT estimates that 9136 new HIV infections would have occurred in Iran in 2010 (95% CI: 6831, 11757). About 56% (95% CI: 47.7%, 61.6%) of new infections were among intravenous drug users (IDUs) and 12% (95% CI: 9.5%, 15%) among their sexual partners. The major routes of direct and indirect HIV transmission in Iran are unsafe injection (68%) and unprotected sexual contact (34% unprotected heterosexual and 10% homosexual) respectively. If current coverage for safe injection among IDUs increases from 80% to 95%, new HIV infections in this group would decrease around 75%. CONCLUSION IDUs remain at highest risk of HIV infection in Iran, so the preventive program coverage for IDUs and their spouses needs to be increased. As the sexual transmission of HIV contributes increasingly to the pool of new infections, serious measures such as harm reduction program are required to reduce sexual transmission of HIV among the relevant key populations.
Predicting job satisfaction and workers’ intentions to leave at the bottom of the high value agricultural chain: evidence from the Ethiopian cut flower industry

Abstract While there is a large body of research focusing on working conditions in labour-intensive industries, none has yet considered how workers themselves perceive their own working conditions. This paper draws on a sample of workers from the cut flower sector industry in Ethiopia to examine the extent to which workers are satisfied with their jobs and to explore the relationship between job satisfaction and intention to leave. Results suggest that a positive evaluation of extrinsic organizational rewards (wage, job security and healthy environment) contributes to workers’ job satisfaction. Dissatisfied workers report higher intentions to leave their job in the flower farms. In addition to low levels of job satisfaction, having alternative livelihood strategies is a key determining factor in the intention to leave. Interestingly, being a member of an informal savings net organized at the work floor mitigates the intention to leave. The paper ends with some implications for managers and policy-makers, along with limitations and suggestions for future research.
Breast cancer in black African women: a changing situation

Black African women in rural South Africa have a very low incidence rate of breast cancer, 5-10 per 100,000. The rate, however, is rising in the considerably increasing urban population. During the period 1994 to 1999 in Durban, enquiries revealed an average of 57 urban patients admitted to hospital each year, from a population of about 600,000 African women, indicating an age-adjusted annual incidence rate of 15.1 per 100,000. This incidence rate is very low in comparison with those in developed populations, which range from 40 to 89 per 100,000. In the African patients studied, the mean age on admission was relatively young, 54.1±10.9 years, almost a decade earlier than patients of developed populations. Moreover, the disease was very far advanced; 21.1% were at Stage III and 63.1% at Stage IV. As to exposure to risk factors, African women in general are characterised by certain protective factors. These factors, which closely resemble those of importance in patients in developed populations are, late menarche, early age at birth of first child, high parity (with usually prolonged lactation), and being physically active. However, with ongoing changes in the lifestyle of urban African women, the protective factors are decreasing in their intensity. Changes in these respects have been associated with rises in the disease’s incidence rate. Clearly, because of the late stage of the disease at the time of the patients’ admission to hospital, and hence their poor survival rate, intensive efforts should be made to educate women to seek help at an early stage of their disease. For its avoidance, feasible protective or restraining measures are primarily to adopt a ‘prudent’ lifestyle, in respect of both dietary and non-dietary components. However, the chances of these measures being meaningfully adopted in African urban communities, unfortunately, are negligible. In consequence, further increases in incidence rate would seem inevitable.
Ray-Optics Modelling of Rectangular and Cylindrical 2-Layer Solar Concentrators

In the present work we quantify the light intensity reaching the side faces of an externally-coated, rectangular luminescent solar concentrator, and the facets of a cylindrical one. Ray-tracing is used: an analytical model has been constructed and discretized. The main novelties reside in the attribution of a finite thickness and attenuation coefficient to the external layer, and in the comparison between two geometries that have been measured against each other only in the homogeneous limit so far. In previous studies the external material is usually treated as infinitely thin. A physical thickness allows, instead, to calculate the ray-paths, to quantify the absorption losses and to evaluate the efficiency of the concentrator as function of the external layer depth. A set of numerical experiments has been performed, in order to evaluate the efficiency of the concentrator when the thickness and material properties of the outer layer are changed, and to compare the performance of the rectangular to the one of the cylindrical device under various conditions. Qualitatively we find the bilayer device to have greater optical efficiency than a comparable homogenous version. For the cylindrical geometry the factor of improvement over the homogenous device is more strongly dependent on both the thickness and the attenuation of the luminescent layer than for the rectangular geometry.
Norwalk Virus N-Terminal Nonstructural Protein Is Associated with Disassembly of the Golgi Complex in Transfected Cells

ABSTRACT Norwalk virus is the prototype strain for members of the genus Norovirus in the family Caliciviridae, which are associated with epidemic gastroenteritis in humans. The nonstructural protein encoded in the N-terminal region of the first open reading frame (ORF1) of the Norwalk virus genome is analogous in gene order to proteins 2A and 2B of the picornaviruses; the latter is known for its membrane-associated activities. Confocal microscopy imaging of cells transfected with a vector plasmid that provided expression of the entire Norwalk virus N-terminal protein (amino acids 1 to 398 of the ORF1 polyprotein) showed colocalization of this protein with cellular proteins of the Golgi apparatus. Furthermore, this colocalization was characteristically associated with a visible disassembly of the Golgi complex into discrete aggregates. Deletion of a predicted hydrophobic region (amino acids 360 to 379) in a potential 2B-like (2BL) region (amino acids 301 to 398) near the C terminus of the Norwalk virus N-terminal protein reduced Golgi colocalization and disassembly. Confocal imaging was conducted to examine the expression characteristics of fusion proteins in which the 2BL region from the N-terminal protein of Norwalk virus (a genogroup I norovirus) or MD145 (a genogroup II norovirus) was fused to the C terminus of enhanced green fluorescent protein. Expression of each fusion protein in cells showed evidence for its colocalization with the Golgi apparatus. These data indicate that the N-terminal protein of Norwalk virus interacts with the Golgi apparatus and may play a 2BL role in the induction of intracellular membrane rearrangements associated with positive-strand RNA virus replication in cells.
On the Different Processing of Wholes and Parts: A Psychophysiological Analysis

The global precedence hypothesis (Navon, 1977) assumes that the processing of the global level of a hierarchical pattern precedes that of the local level. To explore further the nature of global and local processing of compound stimuli, we recorded the event-related brain potentials (ERPs) associated with ident@ing the global and local levels of nonlinguistic compound stimuli in a selective attention task. Wile subjects' behavioral responses were similar to those observed by Navon (1977), the analyses of ERP data showed that identification of the local level elicited longer N2 and P3 peak latencies with enhanced N2 and decreased P3 amplitudes relative to the identification of the global level. The inconsistency between the global and local levels made N2 and P3 amplitudes more negative with longer peak latencies. This interference effect on N2 and P3 amplitude and P3 latency was stronger on the local level than on the global level. The modulation of N2 by the consistency of the global and local levels observed in this and the previous (Heinze, Muente, et al., 1994) study suggests that the interference effect may be mediated by the early perceptual processing. Moreover, we found that the amplitude of an early posterior P1 component was modulated by attention to the global and local levels, being larger to the local target than to the global one. This PI effect gives no support to the notion that the variation of attentional spotlight determines the global precedence effect.
Phytoplankton group dynamics in the Bay of Marseilles during a 2-year survey based on analytical flow cytometry.

BACKGROUND The Bay of Marseilles is under the influence of a large urban concentration and its maritime activities. All of them discharge compounds (hydrocarbons, excess nutrients, heavy metals, chemicals, etc.) that can alter the marine ecosystem. To investigate whether ultraphytoplankton (<10 microm) could be used as biosensors for their own ecosystem, a 2-year survey was conducted in the Bay of Marseilles. METHODS Seven stations monitored water mass and potential anthropic effects in the bay. Seawater samples were collected monthly or bimonthly at three depths, prefiltered, fixed, and kept in liquid nitrogen until flow cytometric analysis. RESULTS Five categories were created: Prochlorococcus, picoeukaryotes (<2 microm), nanoeukaryotes I (2--6 microm), nanoeukaryotes II (6--10 microm), and Synechococcus (<1.5 microm). Artificial neural network analysis (Kohonen self-organizing maps) produced the same number of clusters as cluster analysis with Winlist software (Verity Software House). CONCLUSIONS In addition to the wide variabilities in abundance and biomass, there were a strong seasonal signal and sporadic events. Lessons are derived from this study for future monitoring of marine microorganisms.
Increased levels of a particular phosphatidylcholine species in senescent human dermal fibroblasts in vitro

Plasma membranes are essential components of living cells, and phospholipids are major components of cellular membranes. Here, we used liquid chromatography/mass spectrometry to investigate changes in the membrane phospholipid content that occur in association with aging. Our results indicate that the levels of a particular species of phosphatidylcholine comprised of stearic acid and arachidonic acid increased with age. To determine the reason forthe increased levels of this particular phosphatidylcholine, we examined the effect of highly unsaturated fatty acids, such as arachidonic acid and eicosapentaenoic acid, on cellular aging. Applied arachidonic acid was incorporated into phosphatidylcholine molecules, but neither arachidonic acid nor other related unsaturated fatty acids had any effect. We conclude that increased levels of this distinctive phosphatidylcholine are a result of in vitro senescence.
Identification of Compounds with Bioactivity against the Nematode Caenorhabditis elegans by a Screen Based on the Functional Genomics of the Marine Bacterium Pseudoalteromonas tunicata D2

ABSTRACT Marine bacteria are a rich, yet underexplored, resource of compounds with inhibitory bioactivity against a range of eukaryotic target organisms. Identification of those inhibitors, however, requires a culturable or genetically tractable producer strain, a prerequisite that is not often fulfilled. This study describes a novel functional genomic screen that is based on expression of inhibitors in a heterogeneous recombinant host (i.e., Escherichia coli). Functional libraries were screened by selective grazing by the nematode Caenorhabditis elegans, in a simple, rapid, high-throughput manner. We applied our approach to discover inhibitors of C. elegans produced by the marine bacterium Pseudoalteromonas tunicata D2, a model organism for exploring a range of antagonistic activities between bacteria and eukaryotes and a known producer of several toxic compounds. Expression of P. tunicata DNA in E. coli and grazing selection by the nematode Caenorhabditis elegans identified two clones, with slow- and fast-killing modes of action. Genomic analysis of the slow-killing clone revealed that the activity was due to a small molecule, tambjamine, while the fast-killing activity involved a gene encoding for a novel protein. Microscopic analysis showed substantial colonization of the intestinal lumen, or rapid death of the nematode without colonization, for the two activities, respectively. The novel functional genomic screen presented here therefore detects new eukaryotic inhibitors with different chemical structures, kinetics, and predicted modes of actions.
Progressive fibrosis in nonalcoholic steatohepatitis: association with altered regeneration and a ductular reaction.

BACKGROUND & AIMS Portal fibrosis and linkage is a key feature of progressive disease in nonalcoholic steatohepatitis (NASH), but not simple steatosis. It is underappreciated and poorly understood. Fatty liver has impaired regeneration that induces a secondary replicative pathway using bipotential, periportal, hepatic progenitor cells (HPCs). We propose that activation of this pathway, with increased cell injury in NASH, also induces a periportal ductular reaction (DR) that could produce a profibrogenic stimulus. METHODS Biopsy specimens from 107 patients with nonalcoholic fatty liver disease and 11 controls were immunostained with cytokeratin-7 to quantify the DR and HPCs, and with p21 to assess hepatocyte replicative arrest. These results were correlated with clinicopathologic variables. RESULTS Patients with nonalcoholic fatty liver disease had expansion of HPCs, with a strong association between HPCs and the DR (r(s) = 0.582, P < . 0001). In those with NASH (n = 69) there was an increased DR compared with simple steatosis, which correlated with the stage of fibrosis (r(s) = 0.510, P < . 0001). The DR increased with the grade of NASH activity (r(s) = 0.478, P < . 0001), grade of portal inflammation (r(s) = 0.445, P < . 0001), and extent of hepatocyte replicative arrest (r(s) = 0.446, P < . 0001). Replicative arrest was in turn associated with insulin resistance (r(s) = 0.450, P < . 0001) and NASH activity (r(s) = 0.452, P < . 0001). By multivariate analysis, the extent of DR (odds ratio [OR] = 17.9, P = .016), hepatocyte ballooning (OR = 8.1, P < .0001), and portal inflammation (OR = 3.3, P = .005) were associated independently with fibrosis. CONCLUSIONS These findings suggest that an altered replication pathway in active NASH promotes a periportal DR, which in turn may provoke progressive periportal fibrogenesis.
CD105+-mesenchymal stem cells migrate into osteoarthritis joint: An animal model

Mesenchymal stem cells are being the focus of connective tissue technology and regenerative medicine, presenting a good choice cell source for improving old and well recognized techniques of cartilage defect repair. For instance, the autologous chondrocyte transplantation using new concepts of regenerative medicine. The present study investigated the risk of xenogenicity of human synovial membrane-derived MSCs, injected into the monkeys using intravenous and intra-articular administration. The animal models used were adult monkeys Rhesus which had been injured into the left knee to create an Osteoarthritis (OA) animal model. CD105+-MSCs were injected twice into the OA monkeys with an interval of one week between them. The animals were euthanized one month after treatment. Immunohistochemistry analysis of different organs: spleen, heart, fat, liver, gut, pancreas, lung, skeletal muscle and kidney from the animals revealed that CD105+-MSCs migrated towards the injured knee joint. MSCs naive were found statistically significant increased in the injured knee in front of healthy one. CD105+-MSCs were negatives for CD68 and the area where CD105+-MSCs were found presented SDF-1 increased levels in front of healthy knee. We concluded that a characterized MSCs subset could be a safe alternative for cell therapy in clearly localized pathologies.
Purification, characterization, and lytic activity against Naegleria fowleri of two amoebicins produced by Bacillus licheniformis A12

Bacillus licheniformis A12 produces two amoebolytic substances (amoebicins A12-A and A12-B) in liquid media during sporulation. Both substances have been purified and characterized. They are heat- and protease-resistant peptides containing aspartic acid, glutamic acid, serine, proline, and tyrosine in a molar ratio of 5:2:2:2:2. No fatty acids or carbohydrates have been detected. Their molecular weight is 1,430. Purified amoebicins A12-A and A12-B exhibit amoebolytic action against Naegleria fowleri. They also exhibit antibiotic action against yeasts (Saccharomyces heterogenicus and Cryptococcus neoformans) and several fungal species (Aspergillus niger, Microsporum canis, Mucor plumbeus, and Trychophyton mentagrophytes). Their antibacterial spectrum appears to be restricted to Bacillus megaterium, Corynebacterium glutamicum, and Sarcina sp.
Exploring the Link Between Emotional and Behavioral Dysregulation: A Test of the Emotional Cascade Model

ABSTRACT The emotional cascade model (Selby, Anestis, & Joiner, 2008) posits that the link between emotional and behavioral dysregulation may be through emotional cascades, which are repetitive cycles of rumination and negative affect that result in an intensification of emotional distress. Dysregulated behaviors, such as non-suicidal self-injury, are used to reduce aversive emotions and distract the person from ruminative processes. The aim of the present study was to investigate the emotional cascade model in a non-Western sample of Turkish university students. Accordingly, a structural equation model was tested, and the results demonstrated that the emotional cascades were indeed associated to dysregulated behaviors, even when the effect of current symptoms of depression and anxiety on behavioral dysregulation was statistically controlled. Furthermore, thought suppression had a stronger relationship with all symptom patterns as compared to rumination, which may point to a cultural difference. Possible implications of the findings are discussed.