Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of dopamine on prolactin: interactions with cocaine self-administration by female rhesus monkeys.

The effects of dopamine on regulation of prolactin secretion were studied in female rhesus monkeys before cocaine exposure and again after 2 months to 2.7 years of daily cocaine self-administration. During chronic cocaine exposure, basal prolactin levels increased by 227 to 350% above drug-free control levels (P < .05). On each endocrine study day, three successive 80-min dopamine infusions (10 micrograms/kg/min i.v.) were alternated with 20-min interruptions of dopamine infusions to assess the degree of prolactin suppression and the magnitude of postdopamine prolactin increases. Dopamine significantly reduced prolactin below base-line levels within 60 to 80 min under all conditions (P < .05-.01). In four drug-naive follicular phase females, postdopamine increases in prolactin never exceeded predopamine base-line levels of 6.2 (+/- 1.8) ng/ml. After an average of 74 days of cocaine self-administration [3.7 (+/- 0.11) mg/kg/day], the postdopamine prolactin increases were significantly higher than during drug-free control conditions (P < .01) and reached hyperprolactinemic levels of 57.6 ng/ml. After an average of 300 days of cocaine self-administration [6.5 (+/- 0.06) mg/kg/day], postdopamine prolactin increases peaked at 339% above predopamine basal prolactin levels. After an average of 433 days of cocaine self-administration [6.45 (+/- 0.08) mg/kg/day] postdopamine prolactin increases remained significantly higher (P < .01) than during drug-free conditions. A similar pattern of postdopamine prolactin increases to hyperprolactinemic levels (ranging from 44.5 to 141.2 ng/ml) also were measured in two other females studied after 19 to 20 months of cocaine self-administration [6.21 (+/- 0.11) and 7.49 (+/- 0.17) mg/kg/day]. After 2.7 years of cocaine self-administration, one monkey developed persistent hyperprolactinemia and basal prolactin levels averaged 326 ng/ml after 89 days of cocaine abstinence. These data suggest that the prolactin secretory response to dopamine perturbation may provide a sensitive index of changes in dopaminergic regulation of prolactin during chronic cocaine exposure.
Pilot Study of Pain and Coping Among Patients With Osteoarthritis: A Daily Diary Analysis

Background and Objectives:Few studies have examined patterns of pain and coping among patients with osteoarthritis (OA). This pilot study used a daily diary approach to examine pain and coping strategy use among white and nonwhite veterans with OA. Methods:Participants (23 white, 13 nonwhite; 89% male; mean age = 63 years) completed diaries of pain (10-cm visual analog scale) and coping (total, problem-focused, and emotion-focused) for 30 days. Analyses examined relationships of mean self-reported pain severity and variability with coping strategy use as well as racial differences in these associations. Results:The mean pain level (4.46 [scale of 0–10], standard deviation [SD] = 2.12) and mean within-subject pain variance (1.94, SD = 1.79) were similar between white and nonwhite participants. With respect to pain variability, 2 distinct subgroups were observed, with approximately half of participants reporting high variability and half reporting low variability. The mean total coping score (on a scale of 0–7) was 2.62 (SD = 1.77), with problem-focused strategies being used more often than emotion-focused. There were no significant associations between coping (total, problem-focused, and emotion-focused) and mean pain severity, but the direction of these relationships differed according to race. Conclusion:Results of this pilot study showed considerable between-subject variability in pain and coping strategy use as well as some racial differences. Medical treatment and self-management approaches may be improved if they can be tailored according to patients’ pain patterns and preferred coping strategies.
The Imaging and Evaluation of Body Posture Defects in Hearing Impaired Children

Abstract The paper presents the use of a diagnostics device Posturometer-S applied to examine body posture in hearing impaired children. 46 subjects from the Maria Grzegorzewska School and Education Centre for Hard-of-Hearing Children in Wrocław were examined. The research was conducted through the use of a Posturometer-S, which is an electronic, non-invasive measuring device attached to a computer. It enables not only the imaging and evaluation of frontal and sagittal curves of the spine, but also spinal mobility. Previous research has indicated that incorrect body posture was more often observed in hearing impaired children than in healthy ones. Hence, constant control over body posture in children with hearing loss seems vital. Correct body posture has a great influence on functioning of the entire body. The results obtained by using the Posturometer-S and anthropological measurements helped to determine the types of body posture and postural defects in the examined subjects. The apparatus used in research enabled to determine the occurrence of kyphotic, lordotic and balanced body postures, the results as follows: incorrect kyphotic postures - 10,9% of the subjects, incorrect lordotic postures - 6,4% and incorrect balanced postures - 10,9% of the examined subjects. Correct body postures were observed in 67,4% of the examined children. The most frequent body posture abnormalities were: thoracic scoliosis (19,6%) and shoulder asymmetry (26,1%). Due to the screening tests, some children were referred for further diagnostic evaluation and physiotherapy.
Septicemic invasive Klebsiella pneumoniae infection in a cynomolgus monkey (Macaca fascicularis) with severe diffused suppurative meningoencephalitis

A 2-year-old male cynomolgus monkey (Macaca fascicularis) showed neurological symptoms during quarantine for importation into Japan, and was euthanized due to poor prognosis. Gross anatomical examination revealed a hemorrhagic lesion around the lateral ventricle in the cerebrum. Histologically, severe diffused suppurative meningitis and ventriculitis were detected with numerous Gram-negative bacilli in the cerebrum. Immunohistochemically, the bacilli were positively stained with an antibody against Klebsiella pneumoniae. The bacterium was isolated from the liver, and it was confirmed to be K. pneumoniae by 16S rDNA sequencing. The isolate displayed a hypermucoviscosity phenotype, was positive for the rmpA and k2A genes, and demonstrated multidrug resistance. These results suggest that invasive K. pneumoniae can cause septicemic infection, characterized by severe diffused suppurative meningoencephalitis in monkeys.
Client-Centred Practice: What does it Mean and Does it Make a Difference?

During the past 15 years, occupational therapists in Canada, through the Canadian Association of Occupational Therapists, have worked to develop and implement guidelines for practice of a client-centred approach to occupational therapy. One of the difficulties with the current Guidelines for the Client-Centred Practice of Occupational Therapy is the lack of a definition and discussion of the concepts and issues fundamental to client-centred practice. In this paper, key concepts of client-centred practice: individual autonomy and choice, partnership, therapist and client responsibility, enablement, contextual congruence, accessibility and respect for diversity are discussed. Two practice examples are used to illustrate these ideas and raise issues about obstacles to the practice of client-centred occupational therapy. Research evidence about the effectiveness of client-centred concepts in enhancing client satisfaction, functional outcomes and adherence to health service programmes is reviewed.
Anterior cingulate cortex activation during cognitive interference in schizophrenia.

BACKGROUND Previous studies have reported evidence of structural and functional abnormalities in the anterior cingulate cortex of patients with schizophrenia. METHOD The authors studied 19 male patients with chronic schizophrenia and 15 healthy male comparison subjects with functional magnetic resonance imaging and the novel Multi-Source Interference Task, a task designed to elicit robust dorsal anterior cingulate cortex activation in individual subjects. Group averaged and individual (region-of-interest-based) brain activation patterns were compared during the performance of control and interference trials. RESULTS Performance (reaction times and accuracy) did not differ between healthy subjects and patients with schizophrenia. Comparison of interference and neutral blocks revealed activation in the medial wall of the prefrontal cortex in 93% (N=14) of the healthy subjects and 84% (N=16) of the subjects with schizophrenia. Sixty-seven percent (N=10) of the healthy subjects but only 16% (N=3) of the subjects with schizophrenia displayed maximum medial wall activation within the dorsal anterior cingulate cortex. CONCLUSIONS The Multi-Source Interference Task produced robust activation in the medial wall of the prefrontal cortex during cognitive interference. Analysis of individual activation patterns revealed medial wall abnormalities in schizophrenia patients.
Non-Cell-Autonomous Regulation of Root Hair Patterning Genes by WRKY75 in Arabidopsis1[W]

The WRKY75 transcription factor is expressed in the pericycle and vascular tissues of the root and regulates root hair patterning in a non-cell-autonomous manner. In Arabidopsis (Arabidopsis thaliana), root hairs are formed in cell files over the cleft of underlying cortex cells. This pattern is established by a well-known gene regulatory network of transcription factors. In this study, we show that WRKY75 suppresses root hair development in nonroot hair files and that it represses the expression of TRIPTYCHON and CAPRICE. The WRKY75 protein binds to the CAPRICE promoter in a yeast one-hybrid assay. Binding to the promoter fragment requires an intact WRKY protein-binding motif, the W box. A comparison of the spatial expression of WRKY75 and the localization of the WRKY75 protein revealed that WRKY75 is expressed in the pericycle and vascular tissue and that the WRKY75 RNA or protein moves into the epidermis.
Planning abilities of children aged 4 years and 9 months to 8 ½ years: Effects of age, fluid intelligence and school type on performance in the Tower of London test

The present study investigated the relationship between age and one type of environmental factor, namely, type of school (i.e., private vs. public), and the development of mental planning ability, as measured by the Tower of London (TOL) test. Methods Participants comprised 197 public and 174 private school students, ranging in age from 4 years and 9 months to 8 years and 6 months. Besides the TOL test, students were administered Raven's Colored Matrices. Results Results confirmed the findings of previous studies that both age and school type are important predictors of mental planning. Furthermore, results also suggest that the relationship between type of school and mental planning ability cannot be accounted for by differences in students' fluid intelligence. Conclusion In the present study, the TOL test continued to differentiate public from private school students, even after we controlled for the effect of differences on the Raven test.
A generalized V-shaped multilevel method for large scale floorplanning

In this paper, we propose a generalized V-shaped multilevel floorplanning method with consideration of fixed-outline constraint. The Sequence Pair is used as the floorplan representation. The proposed multilevel method (ML-IARFP) adopts a two-stage structure: top-down partitioning and floorplanning followed by bottom-up merging and refinement. At the first stage, we recursively partition and floorplan the circuits until there are limited number blocks in each sub-circuit. Since we use a multi-partitioning instead of bi-partitioning, general non-slicing floorplan structures are explored in each level, which potentially lead to more effective exploration of the solution space. At the second stage, using a multilevel sequence pair structure, we recursively merge the sub-circuits into bigger circuits and do the refinement. Compared with IMF, Capo 10.2 and IARFP, ML-IARFP obtained the best results under fixed-outline constraints, and compared with IARFP, it achieved 9% wirelength reduction on average and showed a better scalability.
Accuracy of a Bedside Dysphagia Screening: A Comparison of Registered Nurses and Speech Therapists

&NA; Evidence‐based guidelines suggest that stroke patients should be screened for dysphagia before oral intake. The purpose of this study was to validate a dysphagia screening tool comparing registered nurses (RNs) with speech therapists (STs). All stroke unit patients who received predetermined scores on specific items of the National Institutes of Health Stroke Scale were eligible for screening. The trial consisted of three parts (with swallow, cough, and vocal quality observed during each part): 1 teaspoon lemon ice, 1 teaspoon applesauce, and 1 teaspoon water. RNs performed five screenings that were compared with independent screenings performed on the same patient within 1 hour by a speech therapist (ST). Eighty‐three paired screenings were completed, with 94% agreement between the RNs and the STs. This screening identifies patients who are able to swallow and can eat from a safe menu until formally evaluated by an ST while maintaining nothing by mouth (NPO) status for those at risk for aspiration.
Focal uptake of fluorodeoxyglucose by the thyroid in patients undergoing initial disease staging with combined PET/CT for non-small cell lung cancer.

PURPOSE To retrospectively evaluate the prevalence of focal fluorodeoxyglucose (FDG) uptake by the thyroid gland on combined positron emission tomographic (PET) and computed tomographic (CT) scans in patients undergoing staging of newly diagnosed non-small cell lung cancer (NSCLC). MATERIALS AND METHODS Institutional review board approval was obtained, informed consent was waived, and the study was Health Insurance Portability and Accountability Act-compliant. Whole-body PET/CT scans and medical records of 140 consecutive patients with newly diagnosed NSCLC (80 men, 60 women; mean age, 66 years; range, 39-89 years) were retrospectively reviewed by two experienced PET/CT scan readers. Maximum standardized uptake value (SUV) was calculated for FDG-avid thyroid foci. Corresponding thyroid CT findings were recorded in patients with focal increased FDG thyroid uptake. RESULTS PET results showed that six patients (4.3%) had seven foci of increased FDG uptake in the thyroid. Five of the seven foci (in four patients) corresponded to a low-attenuation thyroid lesion on the non-enhanced CT scan. Lesions ranged in diameter from 0.8 to 2.5 cm. Four of the lesions were found to be papillary thyroid cancers at fine-needle aspiration biopsy. The fifth lesion was found to be benign at thyroidectomy. The remaining two patients did not have histologic confirmation of their thyroid lesion because no specific biopsy site was visualized on CT or sonographic images and lesions were considered benign. Maximum SUV of the thyroid cancers ranged from 3.0 to 32.9 (mean, 13.7). Maximum SUV of benign thyroid lesions ranged from 4.6 to 6.2 (mean, 5.4). CONCLUSION Focal thyroid FDG uptake found during the initial staging of NSCLC at PET/CT indicates a high likelihood of primary thyroid cancer.
Noncanonical inflammasomes: Antimicrobial defense that does not play by the rules

Although much research has focused on defining the actions of caspase‐1 containing canonical inflammasomes in promoting host defense, noncanonical inflammasomes have received comparatively little attention. Exciting new concepts have recently emerged detailing their atypical mechanism of activation, importance in defending against cytosolic Gram‐negative pathogens, and role in innate immune defenses of nonmyeloid cells, which has revamped interest in the study of noncanonical inflammmasomes. Here, we will discuss these latest findings about caspase‐4, ‐5, and ‐11 containing inflammasomes in the context of their role in pathogen elimination in mice and humans.
Exchange bias effect in alloys and compounds

The phenomenology of exchange bias effects observed in structurally single-phase alloys and compounds but composed of a variety of coexisting magnetic phases such as ferromagnetic, antiferromagnetic, ferrimagnetic, spin-glass, cluster-glass and disordered magnetic states are reviewed. The investigations on exchange bias effects are discussed in diverse types of alloys and compounds where qualitative and quantitative aspects of magnetism are focused based on macroscopic experimental tools such as magnetization and magnetoresistance measurements. Here, we focus on improvement of fundamental issues of the exchange bias effects rather than on their technological importance.
Biosynthesis of Antibiotic Leucinostatins in Bio-control Fungus Purpureocillium lilacinum and Their Inhibition on Phytophthora Revealed by Genome Mining

Purpureocillium lilacinum of Ophiocordycipitaceae is one of the most promising and commercialized agents for controlling plant parasitic nematodes, as well as other insects and plant pathogens. However, how the fungus functions at the molecular level remains unknown. Here, we sequenced two isolates (PLBJ-1 and PLFJ-1) of P. lilacinum from different places Beijing and Fujian. Genomic analysis showed high synteny of the two isolates, and the phylogenetic analysis indicated they were most related to the insect pathogen Tolypocladium inflatum. A comparison with other species revealed that this fungus was enriched in carbohydrate-active enzymes (CAZymes), proteases and pathogenesis related genes. Whole genome search revealed a rich repertoire of secondary metabolites (SMs) encoding genes. The non-ribosomal peptide synthetase LcsA, which is comprised of ten C-A-PCP modules, was identified as the core biosynthetic gene of lipopeptide leucinostatins, which was specific to P. lilacinum and T. ophioglossoides, as confirmed by phylogenetic analysis. Furthermore, gene expression level was analyzed when PLBJ-1 was grown in leucinostatin-inducing and non-inducing medium, and 20 genes involved in the biosynthesis of leucionostatins were identified. Disruption mutants allowed us to propose a putative biosynthetic pathway of leucinostatin A. Moreover, overexpression of the transcription factor lcsF increased the production (1.5-fold) of leucinostatins A and B compared to wild type. Bioassays explored a new bioactivity of leucinostatins and P. lilacinum: inhibiting the growth of Phytophthora infestans and P. capsici. These results contribute to our understanding of the biosynthetic mechanism of leucinostatins and may allow us to utilize P. lilacinum better as bio-control agent.
Pro-apoptosis and anti-proliferation effects of a recombinant dominant-negative survivin-T34A in human cancer cells.

BACKGROUND Survivin is an attractive target for anti-cancer drug development; however targeting it by small molecules or antibodies is difficult, as survivin is neither a kinase nor a cell surface protein. Protein transduction domain (PTD)-mediated macromolecular therapeutics provides an alternative avenue for targeting survivin. MATERIALS AND METHODS A plasmid expressing a dominant-negative survivin-T34A fused with the immunodeficiency virus protein transduction domain TAT was constructed. The fusion protein was expressed and purified from E. coli. The inhibition of proliferation and induction of apoptosis was tested in human lung carcinoma cell line A549 by directly adding survivin-T34A to the cell culture medium. RESULTS Recombinant survivin-T34A was efficiently expressed and purified by affinity chromatography. It induced cell apoptosis as demonstrated by induction of caspase 3 activation and higher percentage of Annexin V staining, and inhibited cell proliferation as determined by cell number counting. CONCLUSION This functional recombinant protein is promising for development of macromolecular therapeutics targeting survivin.
Seeing glee but hearing fear? emotional Mcgurk effect in Swedish

Video and audio recordings of emotional expressions of a male speaker were used to construct conflicting stimuli in order to perform a McGurk experiment. The results are compared to an almost identical experiment with a female speaker, described in Abelin (2007) in order to see if the earlier results were reproduced. The results from both experiments show that in the McGurk condition the visual channel was in general more reliable than audio at conveying emotions, i. e. the results from Abelin (2007) were reproduced. Most frequently the listeners interpreted in accordance with the visual stimuli, or they heard an emotion which was not present in either video or audio. The emotions happiness and anger showed a dominance for visual perception, and the emotions fear, and to some extent surprise showed a preference for auditive perception, in the McGurk condition. No emotional dimension, i.e. the evaluative (positive‐negative) was connected specifically to the visual or the auditive modality.
Personal and situational variables associated with wind turbine noise annoyance.

The possibility that wind turbine noise (WTN) affects human health remains controversial. The current analysis presents results related to WTN annoyance reported by randomly selected participants (606 males, 632 females), aged 18-79, living between 0.25 and 11.22 km from wind turbines. WTN levels reached 46 dB, and for each 5 dB increase in WTN levels, the odds of reporting to be either very or extremely (i.e., highly) annoyed increased by 2.60 [95% confidence interval: (1.92, 3.58), p < 0.0001]. Multiple regression models had R(2)'s up to 58%, with approximately 9% attributed to WTN level. Variables associated with WTN annoyance included, but were not limited to, other wind turbine-related annoyances, personal benefit, noise sensitivity, physical safety concerns, property ownership, and province. Annoyance was related to several reported measures of health and well-being, although these associations were statistically weak (R(2 )< 9%), independent of WTN levels, and not retained in multiple regression models. The role of community tolerance level as a complement and/or an alternative to multiple regression in predicting the prevalence of WTN annoyance is also provided. The analysis suggests that communities are between 11 and 26 dB less tolerant of WTN than of other transportation noise sources.
The Competitive Ratio for On-Line Dual Bin Packing with Restricted Input Sequences

We consider the On-Line Dual Bin Packing problem where we have a fixed number n of bins of equal size and a sequence of items. The goal is to maximize the number of items that are packed in the bins by an on-line algorithm. An investigation of First-Fit and an algorithm called Log shows that, in the special case where all sequences can be completely packed by an optimal off-line algorithm, First-Fit has a constant competitive ratio, but Log does not. In contrast, if there is no restriction on the input sequences, Log is exponentially better than First-Fit. This is the first separation of this sort with a difference of more than a constant factor. We also design randomized and deterministic algorithms for which the competitive ratio is constant on sequences which the optimal off-line algorithm can pack using at most αn bins, if α is constant and known to the algorithm in advance.