Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization and Function of 3-Hydroxy-3-Methylglutaryl-CoA Reductase in Populus trichocarpa: Overexpression of PtHMGR Enhances Terpenoids in Transgenic Poplar

In the mevalonic acid (MVA) pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is considered the first rate-limiting enzyme in isoprenoid biosynthesis. In this study, we cloned a full-length cDNA from Populus trichocarpa with an open reading frame of 1,734 bp. The deduced PtHMGR sequence contained two HMG-CoA motifs and two NADPH motifs, which exhibited homology with HMGR proteins from other species. Subsequently, truncated PtHMGR was expressed in Escherichia coli BL21 (DE3) cells, and enzyme activity analysis revealed that the truncated PtHMGR protein could catalyze the reaction of HMG-CoA and NADPH to form MVA. Relative expression analysis suggests that PtHMGR expression varies among tissues and that PtHMGR responds significantly to abscisic acid (ABA), NaCl, PEG6000, hydrogen peroxide (H2O2), and cold stresses. We used polymerase chain reaction (PCR) analysis to select transgenic Nanlin 895 poplars (Populus× euramericana cv.) and quantitative reverse-transcription PCR (qRT-PCR) to show that PtHMGR expression levels were 3- to 10-fold higher in transgenic lines than in wild-type (WT) poplars. qRT-PCR was also used to determine transcript levels of methylerythritol phosphate (MEP)-, MVA-, and downstream-related genes, indicating that overexpression of PtHMGR not only affects expression levels of MVA-related genes, but also those of MEP-related genes. We also measured the content of terpenoids including ABA, gibberellic acid (GA), carotenes, and lycopene. PtHMGR overexpression significantly increased ABA, GA, carotene, and lycopene content, indicating that PtHMGR participates in the regulation of terpenoid compound synthesis.
Pre-schooling and academic performance of lower Primary School pupils in Zambezi District, Zambia

The primary objective of this study was to investigate the relationship between pre-schooling and academic performance of pre-schooled lower primary school pupils in Zambezi district, Zambia. The study also assessed whether socio-economic status (SES) of parents, pupils gender (sex), age and parent-child interactive behavior were related to academic performance of pre-schooled and non pre-schooled lower primary school pupils in Numeracy and Literacy. This study employed a quantitative cross sectional design to investigate the relationship between pre-schooling and academic performance of lower primary school pupils in Numeracy and Literacy. The sample size was 240 participants (120 pupils and 120 parents and guardians). This study used Multivariate Stepwise Binary Logistic Regression to analyze the data. The above statistic was used in order to investigate the relationship between pre-schooling and explore whether parents SES, pupils’ gender (sex), age and parent-child interactive behavior influenced the relationship between pre-schooling and academic performance of pre-schooled and non pre-schooled lower primary school pupils. The results indicate that there is a relationship between pre-schooling and performance in Literacy and Numeracy in Grade One and Two. This relationship was statistically significant at p< 0.05. The results also showed that age was related to academic performance. Older pupils performed better than younger pupils. This result was significant at p< 0.05. However, it was shown that there is no significant relationship between socio-economic status of parents, sex, parent-child interactive behavior and academic performance of pupils in Literacy and Numeracy. In conclusion, the study has shown that pre-schooling and age are related to academic performance of pre-schooled lower primary school pupils in Literacy and Numeracy, but SES of parents, sex and parent-child interactive behavior are not related to academic performance of the children in the rural setting of Zambezi where this study was conducted. The study’s recommendations are that the Ministry of Education to scale up the implementation of pre-schools in government schools. This may provide easy access to pre-school education especially in rural areas where pre-schools are very few if any. Parents of children in rural Zambia should actively be encouraged to take their children to pre-school before they start Grade One, since results of this study show that pre-schooling is related to academic performance of lower primary school pupils.
The Role of Gender in the Preconscious Processing of Facial Trustworthiness and Dominance

The present study adopted the breaking continuous flash suppression paradigm (b-CFS) to investigate how Chinese participants process trustworthiness (Experiment 1) and dominance (Experiment 2) at the preconscious level. In addition, we tested whether the gender of a face and the gender of a participant can influence the preconscious processing of facial trustworthiness and dominance. Experiment 1 showed that the least and most trustworthy faces both took significantly less time to break into awareness than neutral faces. In Experiment 2, for female faces, neutral faces took significantly less time to break into awareness than the least and most dominant faces. In both experiments, female faces broke through suppression faster than male faces. In summary, for Chinese participants, the preconscious processing of trustworthiness was not different between male and female faces. However, the preconscious processing of dominance was different between male and female faces.
Natural Killer Cell-Derived IL-10 Prevents Liver Damage During Sustained Murine Cytomegalovirus Infection

Natural Killer (NK) cells are lymphocytes of the innate immune response that play a vital role in controlling infections and cancer. Their pro-inflammatory role has been well-established; however, less is known about the regulatory functions of NK cells, in particular, their production of the anti-inflammatory cytokine IL-10. In this study, we investigated the immunoregulatory function of NK cells during MCMV infection and demonstrated that NK cells are major producers of IL-10 during the early stage of infection. To investigate the effect of NK cell-derived IL-10, we have generated NK cell-specific IL-10-deficient mice (NKp46-Cre-Il10fl/fl) displaying no signs of age-related spontaneous inflammation, with NK cells that show no detectable IL-10 production upon in vitro stimulation. In NKp46-Cre-Il10fl/fl mice, the levels of IL-10 and IFNγ, viral burdens and T cell activation were similar between NKp46-Cre-Il10fl/fl mice and their control littermates, suggesting that NK cell-derived IL-10 is dispensable during acute MCMV infection in immunocompetent hosts. In perforin-deficient mice that show a more sustained infection, NK cells produce more sustained levels of IL-10. By crossing NKp46-Cre-Il10fl/fl mice with perforin-deficient mice, we demonstrated that NK cell-derived IL-10 regulates T cell activation, prevents liver damage, and allows for better disease outcome. Taken together, NK cell-derived IL-10 can be critical in regulating the immune response during early phases of infection and therefore protecting the host from excessive immunopathology.
Developing and Validating a Big-Store Multiple Errands Test

The Multiple Errands Test (MET) is an ecologically valid assessment that characterizes how executive dysfunction manifests in everyday activities. Due to the naturalistic nature of this assessment, clinicians and researchers have had to develop site-specific versions resulting in numerous published versions and making it difficult to establish standard psychometric properties. The aim of this study was to develop a standardized, community version of the MET designed to be used in large department stores meeting set criteria that would not require site specific modifications. This paper reports on the development, content validity, feasibility, and inter-rater reliability of a Big-Store MET, and the performance of healthy participants on this test. Items were selected to match previously published versions in relation to quantity and complexity. Content validity was established by having experts (n = 4) on the MET review the proposed Big-Store version and evaluate the task consistency with previously published versions. To assess feasibility of administration, and inter-rater reliability, a convenience sample of 14 community dwelling adults, self-reporting as healthy, were assessed by two trained raters. We found the Big-Store MET to be feasible to deliver (completed within 30 min, scores show variability, acceptable to participants in community environment) and inter-rater reliability to be very high (ICCs = 0.92–0.99) with the exception of frequency of strategy use. This study introduces the Big-Store MET to the literature, establishes its preliminary validity and reliability thus laying the foundation for a standardized, community-based version of the MET.
Effect of Training with Teaching Methods Designed Based on Health Belief Model on Knowledge and Self-efficacy in Nurses on the Disciplines Standard Precautions in Hospitals

Effect of Training with Teaching Methods Designed Based on Health Belief Model on Knowledge and Self-efficacy in Nurses on the Disciplines Standard Precautions in Hospitals M Khatun Ghanbary1, M Shamsi1, M Khorsandi1, A Farazi2, M Ranjbaran3, B Eshrati3 1 Department of Health Education and Health, Promotion Faculty of Health, Arak University of Medical Sciences, Arak, Iran 2 Department of Infectious Diseases, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran 3 Department of Epidemiology, Faculty of Health, Arak University of Medical Sciences, Arak, Iran Abstract Background: Since education is one of the main elements of prevention and control of nosocomial infections, this study aimed to effect of training with teaching methods designed based on health belief model (HBM) on knowledge and self‑efficacy in nurses on the disciplines and standard precautions were taken. Methods: This is a quasi‑experimental study that was carried out on 100 nurses 50 in experimental and 50 nurses in control group of nurses working hospitals. Data collection tool performed a valid and reliable questionnaire on HBM on preventive behaviours of nosocomial infections. Statistical analysis was performed using t‑test, paired t‑test and Chi‑square. Results: Mean age in the groups of intervention and control was 35.20 ± 7/41 years and 35.02 ± 7/38 , respectively. Before the intervention, mean scores of the knowledge and self‑efficacy of nosocomial infection in the two groups do not have a significant statistical difference. However, after the theory‑based training intervention, the mean scores for these variables have significant statistical differences between the two groups (P < 0.05). Conclusion: According to these study results, executives of the plan support the efficacy of the model on promoting knowledge and self‑efficacy on the disciplines and standard precautions in nurses. Therefore, use of this model is recommended in health centres and hospitals. Key words: Health belief model, knowledge, nosocomial infections, nurses, standard precautions
Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata

We have previously reported on the activity of different extracts from Astronium sp. against Candida albicans, with the hydroethanolic extract prepared from leaves of A. urundeuva, an arboreal species widely distributed in arid environments of South America and often used in folk medicine, displaying the highest in vitro activity. Here we have further evaluated the antifungal activity of this extract against strains of C. albicans and C. glabrata, the two most common etiological agents of candidiasis. The extract was tested alone and loaded into a nanostructured lipid system (10% oil phase, 10% surfactant and 80% aqueous phase, 0.5% Poloxamer 407®). In vitro susceptibility assays demonstrated the antifungal activity of the free extract and the microemulsion against both Candida species, with increased activity against C. glabrata, including collection strains and clinical isolates displaying different levels of resistance against the most common clinically used antifungal drugs. Checkerboard results showed synergism when the free extract was combined with amphotericin B against C. albicans. Serial passage experiments confirmed development of resistance to fluconazole but not to the free extract upon prolonged exposure. Although preformed biofilms were intrinsically resistant to treatment with the extract, it was able to inhibit biofilm formation by C. albicans at concentrations comparable to those inhibiting planktonic growth. Cytotoxicity assays in different cell lines as well as an alternative model using Artemia salina L. confirmed a good safety profile of the both free and loaded extracts, and an in vivo assay demonstrated the efficacy of the free and loaded extracts when used topically in a rat model of vaginal candidiasis. Overall, these results reveal the promise of the A. urundeuva leaves extract to be further investigated and developed as an antifungal.
Cognitive Performance and Psychological Distress in Breast Cancer Patients at Disease Onset

Objective Many cancer patients complain about cognitive dysfunction. While cognitive deficits have been attributed to the side effects of chemotherapy, there is evidence for impairment at disease onset, prior to cancer-directed therapy. Further debated issues concern the relationship between self-reported complaints and objective test performance and the role of psychological distress. Method We assessed performance on neuropsychological tests of attention and memory and obtained estimates of subjective distress and quality of life in 27 breast cancer patients and 20 healthy controls. Testing in patients took place shortly after the initial diagnosis, but prior to subsequent therapy. Results While patients showed elevated distress, cognitive performance differed on a few subtests only. Patients showed slower processing speed and poorer verbal memory than controls. Objective and self-reported cognitive function were unrelated, and psychological distress correlated more strongly with subjective complaints than with neuropsychological test performance. Conclusion This study provides further evidence of limited cognitive deficits in cancer patients prior to the onset of adjuvant therapy. Self-reported cognitive deficits seem more closely related to psychological distress than to objective test performance.
Predicting Optimal Combination LT4 + LT3 Therapy for Hypothyroidism Based on Residual Thyroid Function

Objective: To gain insight into the mixed results of reported combination therapy studies conducted with levothyroxine (LT4) and liothyronine (LT3) between 1999 and 2016. Methods: We defined trial success as improved clinical outcome measures and/or patient preference for added LT3. We hypothesized that success depends strongly on residual thyroid function (RTF) as well as the LT3 added to sufficient LT4 dosing to normalize serum T4 and TSH, all rendering T3 levels to at least middle-normal range. The THYROSIM app was used to simulate “what-if” experiments in patients and study designs corresponding to the study trials. The app graphically provided serum total (T4) and free (FT4) thyroxine, total (T3) and free (FT3) triiodothyronine, and TSH responses over time, to different simulated LT4 and combination LT4 + LT3 dosage inputs in patients with primary hypothyroidism. We compared simulation results with available study response data, computed RTF values that matched the data, classified and compared them with trial success measures, and also generated nomograms for optimizing dosages based on RTF estimates. Results: Simulation results generated three categories of patients with different RTFs and T3 and T4 levels at trial endpoints. Four trial groups had >20%, four <10%, and five 10–20% RTF. Four trials were predicted to achieve high, seven medium, and two low T3 levels. From these attributes, we were able to correctly predict 12 of 13 trials deemed successful or not. We generated an algorithm for optimizing dosage combinations suitable for different RTF categories, with the goal of achieving mid-range normal T4, T3 and TSH levels. RTF is estimated from TSH, T4 or T3 measurements prior to any hormone therapy treatment, using three new nonlinear nomograms for computing RTFs from these measurements. Recommended once-daily starting doses are: 100 μg LT4 + 10–12.5 μg LT3; 100 μg LT4 + 7.5–10 μg LT3; and 87.5 μg LT4 + 7.5 μg LT3; for <10%, 10–20%, and >20% RTF, respectively. Conclusion: Unmeasured and variable RTF is a complicating factor in assessing effectiveness of combination LT4 + T3 therapy. We have estimated and partially validated RTFs for most existing trial data, using THYROSIM, and provided an algorithm for estimating RTF from accessible data, and optimizing patient dosing of LT4 + LT3 combinations for future combination therapy trials.
The Forensic Restrictiveness Questionnaire: Development, Validation, and Revision

Introduction: Forensic psychiatric care is often practiced in closed institutions. These highly regulated, secure, and prescriptive environments arguably reduce patient autonomy, self-expression, and personhood. Taken together these settings are restrictive as patients’ active participation in clinical, organizational, community, and personal life-worlds are curtailed. The consequences of patients’ experiences of restrictiveness have not been explored empirically. This study aimed to develop a psychometrically-valid measure of experiences of restrictiveness. This paper presents the development, validation, and revision of the Forensic Restrictiveness Questionnaire (FRQ). Methods: In total, 235 patients recruited from low, medium, and high secure hospitals across England completed the FRQ. The dimensionality of the 56-item FRQ was tested using Principle Axis Factor Analysis and parallel analysis. Internal consistency was explored with Cronbach’s α. Ward climate (EssenCES) and quality of life (FQL-SV) questionnaires were completed by participants as indicators of convergent validity. Exploratory Factor Analysis (EFA) and Cronbach’s α guided the removal of items that did not scale adequately. Results: The analysis indicated good psychometric properties. EFA revealed a unidimensional structure, suggesting a single latent factor. Convergent validity was confirmed as the FRQ was significantly negatively correlated with quality of life (Spearman’s ρ = −0.72) and ward climate (Spearman’s ρ = −0.61). Internal consistency was strong (α = 0.93). Forty-one items were removed from the pilot FRQ. The data indicate that a final 15-item FRQ is a valid and internally reliable measure. Conclusion: The FRQ offers a novel and helpful method for clinicians and researchers to measure and explore forensic patients’ experiences of restrictiveness within secure hospitals.
The Metabolic Landscape of Lung Cancer: New Insights in a Disturbed Glucose Metabolism

Metabolism encompasses the biochemical processes that allow healthy cells to keep energy, redox balance and building blocks required for cell development, survival, and proliferation steady. Malignant cells are well-documented to reprogram their metabolism and energy production networks to support rapid proliferation and survival in harsh conditions via mutations in oncogenes and inactivation of tumor suppressor genes. Despite the histologic and genetic heterogeneity of tumors, a common set of metabolic pathways sustain the high proliferation rates observed in cancer cells. This review with a focus on lung cancer covers several fundamental principles of the disturbed glucose metabolism, such as the “Warburg” effect, the importance of the glycolysis and its branching pathways, the unanticipated gluconeogenesis and mitochondrial metabolism. Furthermore, we highlight our current understanding of the disturbed glucose metabolism and how this might result in the development of new treatments.
Comparing different techniques of satellite imagery classification to mineral mapping pegmatite of Muiane and Naipa: Mozambique

Several scientific studies with different concept on the mapping of pegmatites have been done in Muiane and Naipa (Mozambique) region. However, none of the studies compare different satellite data and different remote sensing classification algorithms. This study aims to compare the land cover/use classification maps and their accuracies considered sentinel-2, aster, and Landsat OLI imagery. The algorithms employed to evaluate the pegmatites location at Naipa and muiane in alto ligonha pegmatite district were minimum distance (MinD), spectral angle mapper (SAM), and maximum likelihood (ML). The identified features of landscape characteristics selected includes 8 class (kaolinite; montmorillonite; water; built up; bare soil; grasslands; shrubs; isolated bush). The results showed that SAM and MinD algorithms are appropriate for mineralogical mapping validated with ground truth data and geological maps. A kappa index of 0.85 and an overall accuracy (OA) of 80% was obtained for SAM algorithm, and a kappa of 0,80 and OA of 90% for the MinD algorithm. The classification of the images using SAM and mind showed better results for the clays (kaolinite, montmorillonite) visible in both classifications, has also been tested unsupervised classifications or criteria determined by the geologist using an input training dataset in the case of supervised classifications.
Diagnosis and Management of Mixed Gestational Trophoblastic Neoplasia: A Study of 16 Cases and a Review of the Literature

Objective: Mixed gestational trophoblastic neoplasia (GTN) is a rare occurrence that refers to the coexistence of choriocarcinoma and/or placental site trophoblastic tumor and/or epithelioid trophoblastic tumor. The diagnosis and management of mixed GTN are challenging. We investigated the clinicopathological characteristics, diagnoses, treatments, and outcomes of patients with mixed GTN. Materials and Methods: The medical records and pathological sections of 16 patients with mixed GTN who were treated at Peking Union Medical College Hospital and The Second Xiangya Hospital of Central South University between January 2012 and December 2018 were reviewed. Results: Pretreatment serum human chorionic gonadotropin (hCG) levels ranged from 180 to 625,024 IU/L, and were >10,000 IU/L in 14 of the 16 patients, none of whom were diagnosed correctly at initial presentation. Two patients were diagnosed with choriocarcinoma coexisting with intermediate trophoblastic tumor (ITT) through dilation and curettage (D&C) before treatment. Another 5 patients were histologically confirmed to have placental site trophoblastic tumor (PSTT) by D&C but final pathological findings showed mixed PSTT and choriocarcinoma at subsequent hysterectomy. Seven post-chemotherapy patients with an initial clinical diagnosis of choriocarcinoma underwent surgery because of chemoresistance and their pathological findings revealed coexisting ITT. The remaining 2 patients were found to have choriocarcinoma coexisting with ITT following cervical biopsy and pulmonary lobectomy. All patients received chemotherapy: 14 underwent surgery combined with chemotherapy and 2 received chemotherapy alone to preserve fertility. Other than 1 patient who died of disease progression, 15 patients (93.8%) achieved complete remission (CR) after treatment, although 5 (33.3%) relapsed. Of these 5 patients with relapse, 3 achieved CR after additional treatment, 1 was receiving an immune checkpoint inhibitor, and 1 was lost to follow-up after refusing further therapy. Conclusion: Mixed GTN is difficult to diagnose on initial presentation. Overlap of the ITT component should be considered in refractory chemoresistant choriocarcinoma. Coexistence of choriocarcinoma should be suspected in ITT patients with high hCG levels. Surgery combined with chemotherapy is optimal treatment for choriocarcinoma mixed with ITT.