Miracle-dz/UniFAR-FTUs huggingface dataset:
Induction of basic fibroblast growth factor mRNA by basic fibroblast growth factor in Müller cells.

PURPOSE To investigate the induction of basic fibroblast growth factor (bFGF) gene expression in cultured rat Müller cells by bFGF and to study the mechanism of induction. METHODS Müller cells from 1- to 3-day-old Sprague-Dawley rats were isolated and cultured with Dulbecco's modified Eagle's medium with 10% fetal calf serum. Cultured cells were identified by immunocytochemistry using antibodies against vimentin, carbonic anhydrase II, and glutamine synthetase. Cells of passages 1 through 4 were treated with bFGF, the protein kinase C (PKC) inhibitor, H-7; calphostin C, or the PKC activator, PMA; and protein kinase A (PKA) inhibitor, H-89; as well as the adenylate cylase activator, forskolin; or the adenylate cyclase inhibitor, SQ22536. Northern blot analysis was performed to determine the mRNA expression of bFGF, ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF). RESULTS Addition of bFGF to culture medium induced bFGF gene expression in a dose- and time-dependent manner. Induction of bFCF mRNA started at a bFGF concentration of 0.1 ng/ml. The bFGF mRNA level was elevated by 2-fold at 1 ng/ml of bFGF, 2.8-fold at 5 ng/ml, and reached a peak of 4-fold at 10 ng/ml and 3.7-fold at 50 ng/ml. At 10 ng/ml of bFGF, induction of bFGF mRNA was observed as early as 2 hours (2-fold) after treatment. The bFGF mRNA level continued to increase to 3.7-fold by 4 hours, and reached a maximum of 4.4-fold by 8 hours. A slow decline of the bFGF mRNA level was observed after 8 hours of bFGF treatment (3.5-fold by 12 hours, and 3-fold by 24 hours). This induction of bFGF gene expression was blocked by PKC inhibitors H-7 (30 microM). The PKC activator PMA (0.1 microM) also upregulated bFGF gene expression, but the effects of bFGF and PMA were not additive. An adenylate cyclase inhibitor, SQ22536 (100 microM), did not inhibit bFGF-induced bFGF gene expression. Although forskolin (5 microM), an adenylate cyclase activator, also upregulated the level of bFGF mRNA, the effects of forskolin and bFGF were additive. In addition, no inhibitory effect on bFGF-induced expression of bFGF mRNA was found using H-89 (1 microM). Exogenous bFGF did not alter the mRNA levels of CNTF and BDNF. CONCLUSIONS These results indicate that bFGF induces bFGF gene expression in cultured rat Müller cells through PKC activation. The authors' findings raise the possibility that Müller cells in vivo also respond to available bFGF (for example, that released from the endogenous reservoirs in the case of injury) or to exogenous bFGF by producing more bFGF, which could in turn promote photoreceptor survival.
Abnormal neurons in teratomas in NMDAR encephalitis.

IMPORTANCE Ovarian teratomas are frequently described in patients with N-methyl-d-aspartate receptor (NMDAR) encephalitis, yet NMDAR encephalitis is rarely described in patients with ovarian teratomas. Understanding why a minority of patients with teratomas are seen with autoimmune encephalitis may improve the management of NMDAR encephalitis and other teratoma-associated autoimmune diseases. OBJECTIVE To characterize the unique organization of neuroglial elements within ovarian teratomas resected from patients with NMDAR encephalitis. DESIGN Case-control study comparing the pathological features of ovarian teratomas resected from consecutively accrued cases with NMDAR encephalitis between January 1, 2009, and December 15, 2013, and ovarian teratomas resected from controls between June 1, 2012, and June 30, 2013. SETTING Pathology tissue database at a tertiary academic care center. PARTICIPANTS Five cases with teratoma-associated NMDAR encephalitis and serum or cerebrospinal fluid autoantibodies against central nervous system (CNS) NMDAR and 38 controls (39 ovarian teratomas) without neurological symptoms or signs. EXPOSURES Formalin-fixed, paraffin-embedded ovarian teratomas were examined for the presence of CNS tissue and inflammatory infiltrates using direct microscopy, enhanced with standard histopathological and immunological stains. MAIN OUTCOMES AND MEASURES Frequency of detection of atypical (dysplastic) CNS neuronal elements in ovarian teratomas resected from cases vs controls, as well as characterization of the relationship between atypical neurons and immune infiltrates. RESULTS Central nervous system neuronal elements were detected in 4 of 5 teratomas resected from cases with NMDAR encephalitis and in 20 of 39 controls (P = .36). Atypical neurons were seen within teratomas resected from 4 of 5 cases but not in 39 controls, reliably distinguishing teratomas associated with NMDAR encephalitis (P < .001). If found within the CNS, these histological abnormalities would have received the diagnosis of gangliogliomas (n = 3) and ganglioneuroblastoma (n = 1). Reactive changes were present in teratomas from controls, including ferruginated neurons and Rosenthal fibers. Abnormal neuroglial elements were closely related to immune infiltrates in teratomas resected from 4 of 4 cases. Inflammatory infiltrates were not associated with neuroglial tissue in 20 controls, further differentiating these populations (P < .001). CONCLUSIONS AND RELEVANCE Abnormal neurons within teratomas distinguish cases with NMDAR encephalitis from controls and may promote the development of autoimmunity.
Silent dissemination of HTLV-1 in an endemic area of Argentina. Epidemiological and molecular evidence of intrafamilial transmission

Background Molecular and epidemiological studies of transmission routes and risk factors for infection by HTLV-1 are extremely important in order to implement control measures, especially because of the high prevalence of HTLV-1 in several regions of the world. San Salvador de Jujuy, Northwest Argentina, is a highly endemic area for HTLV-1 and foci of tropical spastic paraparesis/HTLV-1-associated myelopathy. Objective To gain further insight into the role of intrafamilial transmission of HTLV-1 in a highly endemic region in Argentina. Method Cross-sectional study in Northwest Argentina. Epidemiological data and blood samples were collected from 28 HTLV-1 infected subjects (index cases) and 92 close relatives/cohabitants. HTLV-1 infection was diagnosed by detection of antibodies and proviral DNA. The LTR region was sequenced and analyzed for genetic distances (VESPA software), in addition to determination and identification of polymorphisms to define HTLV-1 family signatures. Results Fifty seven of the 120 subjects enrolled had antibodies against HTLV-1 and were typified as HTLV-1 by PCR. The prevalence rate of HTLV-1 infection in family members of infected index cases was 31.52% (29/92). The infection was significantly associated with gender, age and prolonged lactation. Identity of LTR sequences and presence of polymorphisms revealed high prevalence of mother-to-child and interspousal transmission of HTLV-1 among these families. Conclusion There is an ongoing and silent transmission of HTLV-1 through vertical and sexual routes within family clusters in Northwest Argentina. This evidence highlights that HTLV-1 infection should be considered as a matter of public health in Argentina, in order to introduce preventive measures as prenatal screening and breastfeeding control.
The quarter milk flow parameters influenced by stage of lactation and milkability in multiparous dairy cows.

The effect of stage of lactation, peak flow rate, parity, bimodality and teat position on quarter milk production and milk flow parameters was studied. A total of 25 Holstein multiparous cows (in their second to sixth lactation) were investigated during ten months of lactation. Quarter milk flows were recorded daily at morning (5:30 h) and evening (15:30 h) milking. In total more than 52 000 of quarter flow curves were obtained. The peak milk yield was reached at second month of lactation. Peak flow rate was relatively stabile with slight reduction after seventh month of lactation but mean flow rate continuously reduced. Increase phase tended to increase throughout lactation. Decline phase decreased from the first to second month and then from fourth month continuously increased. Overmilking phase increased from first to third month and then decreased. There was no relation between peak flow and milk yield, milk yield of plateau phase and duration of increase phase. Quarters with high peak flow had longer decline and shorter overmilking as compared with low peak flow ones. All parameters were higher during morning milking except the duration of increase and decline phases, where data were higher during evening milking. Quarter with bimodal milk flow showed lower milk yield and higher peak flow, longer increase and decline phases. Quarter position influenced all parameters of milk yield and milk flow. Front quarter had shorter increase and decline and longer overmilking phases than rear ones. Quarter milk flow traits deserve further investigation to give new knowledge if biological needs of quarter should be considered in developing new machines.
Feeding and adrenal entrainment stimuli are both necessary for normal circadian oscillation of peripheral clocks in mice housed under different photoperiods

The mammalian circadian rhythm is entrained by multiple factors, including the light–dark cycle, the organism’s feeding pattern and endocrine hormones such as glucocorticoids. Both a central clock (the suprachiasmatic nucleus, or SCN) and peripheral clocks (i.e. in the liver and lungs) in mice are entrained by photoperiod. However, the factors underlying entrainment signals from the SCN to peripheral clocks are not well known. To elucidate the role of entrainment factors such as corticosterone and feeding, we examined whether peripheral clock rhythms were impaired by adrenalectomy (ADX) and/or feeding of 6 meals per day at equal intervals under short-day, medium-day and long-day photoperiods (SP, MP and LP, respectively). We evaluated the waveform and phase of circadian rhythms in the liver, kidney and salivary gland by in vivo imaging of PER2::LUCIFERASE knock-in mice. In intact mice, the waveforms of the peripheral clocks were similar among all photoperiods. The phases of peripheral clocks were well adjusted by the timing of the “lights-off”-operated evening (E) oscillator but not the “lights-on”-operated morning (M) oscillator. ADX had almost no effect on the rhythmicity and phase of peripheral clocks, regardless of photoperiod. To reduce the feeding-induced signal, we placed mice on a restricted feeding regimen with 6 meals per day (6 meals RF). This caused advances of the peripheral clock phase in LP-housed mice (2–5 h) and MP-housed mice (1–2 h) but not SP-housed mice. Thus, feeding pattern may affect the phase of peripheral clocks, depending on photoperiod. More specifically, ADX + 6 meals RF mice showed impairment of circadian rhythms in the kidney and liver but not in the salivary gland, regardless of photoperiod. However, the impairment of peripheral clocks observed in ADX + 6 meals RF mice was reversed by administration of dexamethasone for 3 days. The phase differences in the salivary gland clock among SP-, MP- and LP-housed mice became very small following treatment with ADX + 6 meals RF, suggesting that the effect of photoperiod was reduced by ADX and 6 meals RF. Because the SCN rhythm (as evaluated by PER2 immunohistochemistry) was not disrupted by ADX + 6 meals RF, impairment of peripheral clocks in these mice was not because of impaired SCN clock function. In addition, locomotor activity rhythm and modifications of the feeding pattern may not be completely responsible for determining the phase of peripheral clocks. Thus, this study demonstrates that the phase of peripheral clocks responds to a photoperiodic lights-off signal, and suggests that signals from normal feeding patterns and the adrenal gland are necessary to maintain the oscillation and phase of peripheral clocks under various photoperiods.
Self-Attention as a Mediator of Cultural Influences on Depression

Background: Despite the widespread recognition of cultural differences in depressive symptoms, it is unclear through what processes culture affects depressive symptoms. Aim: This research aims at examining whether self-attention mediates the influence of acculturation on changes in depressive symptoms in an immigrant group. Method: Adults of Asian cultural backgrounds were surveyed. Depressive symptoms were assessed and divided into three categories: affective, somatic and interpersonal items of depression. Acculturation experiences were measured. Self-attention to three aspects (i.e. affective, somatic and interpersonal) of the self was also assessed. Results: As acculturation proceeds, Asian immigrants tend to pay increasingly more attention to the affective aspect of the self and less to the somatic aspect of the self. The changes in the focus of self-attention seem to explain the changes in the experience of depression that include more affective and less somatic symptoms of depression as the degree of acculturation increases. Conclusion: Self-attention to certain aspects of the self seems to play an important role mediating the influence of acculturation on changes in subcategories of depressive symptoms.
Sustained cycling exercise increases intracortical inhibition.

PURPOSE In the current study, we measured EMG suppression induced by subthreshold transcranial magnetic stimulation (TMS) to investigate the effects of sustained cycling exercise on intracortical inhibition. METHODS Sixteen subjects cycled at 75% of their maximum workload (Wmax) for 30 min, during which subthreshold TMS was applied at a defined crank angle where vastus lateralis (VL) EMG amplitude was increasing and approximately 50% of its recorded maximum. Subthreshold TMS was also applied during nonfatiguing control cycling bouts at 75% and 37.5% of Wmaxbefore sustained cycling. RESULTS Although EMG in VL during control cycling at 37.5% Wmax was approximately half that during cycling at 75% Wmax (P ≤ 0.05), the amount of EMG suppression was not different between workloads (P > 0.05). EMG amplitude in VL recorded in the last 5 min of sustained cycling was not different from the first 5 min (P > 0.05), whereas the amount of EMG suppression at the end of the sustained cycling was significantly greater than that at the start (P ≤ 0.05). CONCLUSIONS The increase in TMS-evoked EMG suppression during sustained cycling implies an increase in the excitability of the intracortical inhibitory interneurons during the exercise. The observed increase in intracortical inhibition is similar to that observed during sustained single joint contractions, suggesting that changes in the responsiveness of intracortical inhibitory interneurons are similar during locomotor exercise and static single joint contractions.
Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches

Cyclin-dependent kinase 1 (Cdk1) kinase dephosphorylation and activation by Cdc25 phosphatase are essential for mitotic entry. Activated Cdk1 phosphorylates Cdc25 and other substrates, further activating Cdc25 to form a positive feedback loop that drives the abrupt G2/mitosis switch. Conversely, mitotic exit requires Cdk1 inactivation and reversal of Cdk1 substrate phosphorylation. This dephosphorylation is mediated, in part, by Clp1/Cdc14, a Cdk1-antagonizing phosphatase, which reverses Cdk1 phosphorylation of itself, Cdc25, and other Cdk1 substrates. Thus, Cdc25 phosphoregulation is essential for proper G2–M transition, and its contributions to cell cycle control have been modeled based on studies using Xenopus and human cell extracts. Because cell extract systems only approximate in vivo conditions where proteins interact within dynamic cellular environments, here, we use Schizosaccharomyces pombe to characterize, both experimentally and mathematically, the in vivo contributions of Cdk1-mediated phosphorylation of Cdc25 to the mitotic transition. Through comprehensive mapping of Cdk1 phosphosites on Cdc25 and characterization of phosphomutants, we show that Cdc25 hyperphosphorylation by Cdk1 governs Cdc25 catalytic activation, the precision of mitotic entry, and unvarying cell length but not Cdc25 localization or abundance. We propose a mathematical model that explains Cdc25 regulation by Cdk1 through a distributive and disordered phosphorylation mechanism that ultrasensitively activates Cdc25. We also show that Clp1/Cdc14 dephosphorylation of Cdk1 sites on Cdc25 controls the proper timing of cell division, a mechanism that is likely due to the double negative feedback loop between Clp1/Cdc14 and Cdc25 that controls the abruptness of the mitotic exit switch.
Implementation and Assessment Challenges in Iranian Secondary High School EFL Program

Paradigm shift in language education has caused many countries including Iran to reshuffle their public EFL educational program. However, such a promising trend in Iran has entailed serious challenges, which necessitated this study aimed at collecting data from 70 teachers and 70 students to investigate the CLT challenges in terms of both teachers' pedagogical and assessment areas. A Partial Least Square Model (PLSM) addressed these two variables. The findings showed that the first three sub-traits of both CLT and testing knowledge have varying contributions to the main latent variables, respectively. Second, Friedman test indicated lack of compatibility between the respective dimensions as to the implementation of the program. Third, the post-hoc comparison tests revealed that teachers perceived CLT significantly lower than the three areas of CLT testing, knowledge of CLT tests and knowledge of developing CLT tests. Also, their knowledge of characteristics of CLT was significantly lower than their perceptions of CLT testing, and knowledge of CLT tests. However, their knowledge of the characteristics of CLT was not significantly different from that of developing CLT tests. Finally, their knowledge of techniques in CLT was significantly lower than their perceptions of CLT testing, knowledge of CLT tests, and knowledge of developing CLT tests. In sum, the Iranian public EFL program still suffers from both implementation and assessment challenges necessitating remedial teacher education program.
Impact of Breast Cancer Subtypes and Treatment on Survival: An Analysis Spanning Two Decades

Background: We investigated the impact of breast cancer molecular subtypes and treatment on survival in a cohort of medically insured women followed for more than 20 years. Methods: We examined 934 female members of an integrated health care delivery system newly diagnosed with invasive breast cancer between 1988 and 1995 and followed them through 2008. Tumors were classified into four molecular subtypes on the basis of their expression profile: luminal A; luminal B; basal-like; and HER2-enriched. We followed women from the surgery date to death, health plan disenrollment, or study's end. HR and 95% confidence intervals (CI) were fit using Cox proportional hazards models adjusting for cancer treatments and tumor characteristics. Results: A total of 223 (23.9%) women died because of breast cancer during the 21-year study period. Compared with women with luminal A tumors, women with HER2-enriched (HR 2.56, 95% CI 1.53–4.29) and luminal B tumors (HR 1.96, 95% CI: 1.08–3.54) had roughly a two-fold increased adjusted risk of breast cancer mortality. In addition, the survival curves suggest that risk of late mortality persists in women with luminal A tumors. Conclusion: Among women with health care coverage, molecular subtypes were important predictors of breast cancer mortality. Women with HER2-enriched tumors and luminal B subtypes had the poorest survival despite adjusting for important covariates. Impact: In a cohort followed for more than 20 years, women with HER2-enriched tumors had worse survival, but interestingly, the survival curve for women with luminal A tumors continued to steadily decline after 10 years of follow-up. Cancer Epidemiol Biomarkers Prev; 21(10); 1848–55. ©2012 AACR.
Radioembolisation with Yttrium‐90 microspheres: An effective treatment modality for unresectable liver metastases

To compare the outcomes (survival and adverse events) of the authors' use of 90Y microsphere radioembolisation in patients with chemotherapy‐refractory liver metastases with published data from other groups using radioembolisation. To retrospectively evaluate the efficiency, in particular survival benefits, of radioembolisation in the treatment of liver metastases.
Effect of cyclic and static tensile loading on water content and solute diffusion in canine flexor tendons: An in Vitro study

This study was designed to determine the effects of various loading conditions (no load and static and cyclic tensile load) on the water content and pattern of nutrient diffusion of canine flexor tendons in vitro. Region D (designated by Okuda et al.) of the flexor digitorum profundus was subjected to a cyclic or static tensile load of 100 g for times ranging from 5 minutes to 24 hours. The results demonstrated a statistically significant loss of water in tendons subjected to both types of load as compared with the controls (no load). This loss appeared to progress with time. However, neither static nor cyclic loading appeared to alter the diffusion of 3H‐glucose into the tendon over a 24‐hour period compared with the controls. These results suggest that any benefit in tendon repair derived from intermittent passive motion is probably not a result of an increase in the diffusion of small nutrients in response to intermittent tensile load.
Partir ou rester? : les destinées des jeunes issus de l'immigration étrangère en France

Un depart durable vers l'etranger ou l'integration dans la societe francaise sont les deux principales alternatives caracterisant les trajectoires de nos concitoyens, en particulier de ceux dont les histoires familiales ou individuelles se sont caracterisees par des migrations. Un nombre d'ailleurs important d'enfants immigres ayant vecu en France l'ont quittee, pour vivre ailleurs, souvent dans le pays d'origine des parents. Cependant, parmi les personnes issues des familles ou les parent sont ou furent des immigres, une large majorite des jeunes adultes s'etablissent definitivement en France. Par un cheminement quasi-chronologique, ce livre etudie leurs cursus scolaires, leurs rapports a la citoyennete, leurs choix de vie familiaux, le fecondite des femmes, les situations d'activite, d'inactivite ou de recherche d'emploi. La presence et l'integration des personnes provenant de pays etrangers ou issus de l'immigration sont toujours l'objet d'interrogations et controverses, au point d'etre devenues, dans la societe francaise, un des themes majeurs des debats sociaux et politiques. Pourtant, les etudes consacrees aux fils et filles d'immigrees en France et fondees sur des donnees fiables sont demeurees peu nombreuses. L'apport des donnees longitudinales, qui permettent d'etudier les trajectoires et les departs hors de France, permet les analyses totalement inedites proposees dans ce volumes Ce livre interessera non seulement les etudiants et chercheurs en sciences sociales mais aussi de nombreux enseignants, praticiens de l'action sociale, elus, syndicalistes et responsables associatifs.