Miracle-dz/UniFAR-FTUs huggingface dataset:
Prevalence, morphological characterization, and associated factors of anemia among children below 5 years of age attending St. Mary’s Hospital Lacor, Gulu District, Northern Uganda

Aim/objective The aim of this study was to determine the prevalence, severity, morphological characterization, and the associated factors of anemia among children under the age of 5 years at St. Mary’s Hospital Lacor, Gulu District, Northern Uganda. Materials and methods A structured questionnaire was administered to each participant’s parent/caregiver to collect data on sociodemographic factors, feeding pattern, and history of chronic illness. Hemoglobin (Hb) estimation was performed using a HemoCue 201+ analyzer. Peripheral thin and thick blood films were made from venous blood and stained with Giemsa to morphologically characterize red blood cells (RBCs) and investigate hemoparasites, respectively. We collected and examined stool specimens from each participant using wet preparations and formol–ether concentration technique for intestinal parasites. Descriptive statistics was used to describe study participants and to determine the prevalence of anemia. Logistic regression analysis was done to determine the factors associated with acquiring anemia at a P-value≤ 0.05. Results The study enrolled 343 children below the age of 5 years. Of these, 62.7% (N=215) were females. The IQR, median, and mean Hb levels were 5.1±3.2 g/dL, 8.2 g/dL, and 7.9 g/dL, respectively. Overall, 160 (46.6%, 95% CI: 42.1–51.46) children had anemia. The magnitude of severe, moderate, and mild anemia was 11.9%, 58.8%, and 29.4%, respectively. Morphologic characterization of anemia revealed hypochromic-microcytic (65.4%, N=106), hypochromic-macrocytic (15.4%, N=25), and normochromic-microcytic (19.1%, N=31) anemia. Factors associated with anemia were parasitic infestation, history of chronic disease, lack of complementary foods, complementary feeding for not more than twice a month, and households’ with annual income less than 200,000 Ugandan Shillings. Conclusion We report the high prevalence of anemia among children below 5 years of age in Gulu District, Northern Uganda. Thus, strategies geared at addressing the etiologic causes (such as, nutrient deficiency and parasitic infections) are key to reduce it in the region.
RISK FACTORS FOR EARLY HOSPITAL READMISSION FOLLOWING TOTAL KNEE ARTHROPLASTY

ABSTRACT Objective To identify independent risk factors, complications and early hospital readmission following total knee arthroplasty. Methods Using the ACS-NSQIP database, we identified patients who underwent primary TKA from 2012-2015. The primary outcome was early hospital readmission. Patient demographics, preoperative comorbidities, laboratory data, operative characteristics, and postoperative complications were compared between readmitted and non-readmitted patients. Logistic regression identified independent risk factors for 30-day readmission. Results 137,209 patients underwent TKA; 3.4% were readmitted within 30 days. Advanced age, male sex, black ethnicity, morbid obesity, presence of preoperative comorbidities, high ASA classification, and increased operative time were independently related risk factors. Asian and no reported race were negative risk factors. Postoperative complications: acute myocardial infarction, acute renal failure, stroke, pneumonia, pulmonary embolism, and deep vein thrombosis show positive associations. Conclusions Advanced age, male sex, black ethnicity, morbid obesity, presence of comorbidities, high ASA classification and long operative time are independent risk factors for postoperative complications and early hospital readmission following total knee arthroplasty. Level of Evidence III, Case control study.
DOA estimation in Cyclic Prefix OFDM Systems in LOS mmWave Channel using Monopulse Ratio

In millimeter wave (mmWave) systems, analog beam steering (i.e., direction of arrival (DOA) estimation) is an essential operation for subsequent channel estimations. In this paper, we propose a framework of incorporating a monopulse ratio (MR) concept, originally developed for radar systems, into an orthogonal frequency division multiplexing (OFDM) mmWave systems with cyclic prefix (CP) for DOA estimation. It is shown that CP can be used for DOA estimation in general OFDM mmWave systems by employing the MR concept. Based on performance analysis and simulation results, the proposed method can be considered as a practical and robust DOA estimation method for single RF chain analog beamforming systems.
Quantifying Human Behavior on the Block Design Test Through Automated Multi-Level Analysis of Overhead Video

The block design test is a standardized, widely used neuropsychological assessment of visuospatial reasoning that involves a person recreating a series of given designs out of a set of colored blocks. In current testing procedures, an expert neuropsychologist observes a person's accuracy and completion time as well as overall impressions of the person's problem-solving procedures, errors, etc., thus obtaining a holistic though subjective and often qualitative view of the person's cognitive processes. We propose a new framework that combines room sensors and AI techniques to augment the information available to neuropsychologists from block design and similar tabletop assessments. In particular, a ceiling-mounted camera captures an overhead view of the table surface. From this video, we demonstrate how automated classification using machine learning can produce a frame-level description of the state of the block task and the person's actions over the course of each test problem. We also show how a sequence-comparison algorithm can classify one individual's problem-solving strategy relative to a database of simulated strategies, and how these quantitative results can be visualized for use by neuropsychologists.
SEIGAN: Towards Compositional Image Generation by Simultaneously Learning to Segment, Enhance, and Inpaint

We present a novel approach to image manipulation and understanding by simultaneously learning to segment object masks, paste objects to another background image, and remove them from original images. For this purpose, we develop a novel generative model for compositional image generation, SEIGAN (Segment-Enhance-Inpaint Generative Adversarial Network), which learns these three operations together in an adversarial architecture with additional cycle consistency losses. To train, SEIGAN needs only bounding box supervision and does not require pairing or ground truth masks. SEIGAN produces better generated images (evaluated by human assessors) than other approaches and produces high-quality segmentation masks, improving over other adversarially trained approaches and getting closer to the results of fully supervised training.
Cytoskeleton Structure in Mouse Sperm and Testes After 30 Days of Hindlimb Unloading and 12 Hours of Recovery

Background/Aims: Changes in the external mechanical field result in cytoskeleton reorganization and the formation of adaptive patterns in different types of cells, including somatic cells and sex cells. The aim of this research was to study the protein and mRNA content of cytoskeletal and sperm-specific genes in the sperm and testis cells of mice. Methods: Mice were subjected to 30 days of antiorthostatic suspension to simulate weightlessness, followed by 12 h of recovery, while receiving essential phospholipids at a dosage of 500 mg/kg/day (30HSE and 30HSE+12h groups) or a similar dosage of a placebo (30HS and 30HS+12h groups). Accordingly, reference groups (CE group and C group) were formed. The total number and the percentage of motile spermatozoa were calculated using a Makler chamber. To analyze the number of viable spermatozoa and the permeability of their membranes, eosin staining was used as well as Diff-Quick for a morphological evaluation. Relative protein and mRNA content was estimated in a western blot and quantitative PCR assay, respectively. Results: The relative protein expression levels of actin (beta and gamma) and two alpha-actinin isoforms (1 and 4) remained constant in the sperm of all study groups, except for the 30HS+12h group, where the alpha-actinin-4 level was 13% higher than in the reference group (p < 0.1). In the testis cells, the relative actin isoform content was equivalent to that in the spermatozoa. However, in the testis cells, the ACTN1 mRNA content was 17% higher in the 30HS group than in the C group (p < 0.05), and decreased after 12 h of recovery. In contrast, the ACTN4 mRNA content was 20% lower in the 30HS group than in the reference group (p < 0.05) and increased after the 12-h recovery period. At the same time, in the group administered the essential phospholipids, the relative ACTN1 and ACTN4 mRNA content did not differ from those of the reference group. The relative beta-tubulin content was similar in the reference C group and the reference CE group, which was administered the essential phospholipids. In the 30HS and 30HS+12h groups, the beta-tubulin content decreased by 19% and 22% (p < 0.05), respectively, and they also decreased in the groups administered the essential phospholipids (30HSE and 30HSE+12h groups, by 27% and 33%, respectively, p < 0.05). In the testis tissue, the relative tubulin content did not change in any of the experimental groups. At the same time, the relative mRNA content of the genes encoding the studied cytoskeletal proteins increased, which may indicate the protein content was regulated mainly at the translational level. Conclusion: The spermogram parameters and the content of the sperm-specific proteins and the associated mRNAs revealed a decrease in the number of mature spermatozoa in mice suspended under conditions of weightlessness. Moreover, the decrease was prevented by the administration of essential phospholipids.
QUIC and TCP: A Performance Evaluation

Current Internet transport protocols are being revisited in an attempt to respond to traffic growth in size, diversity and the emergence of new applications. Designed to reduce Web TCP latency and connection establishment time, QUIC uses UDP and defines its own congestion control. To verify some of QUIC’s performance claims, we carried out an extensive set of controlled experiments reflecting Internet traffic conditions by configuring various parameters, such as the round trip time (RTT), the packet loss ratio, web pages, and caching. Surprisingly, our experimental results show that QUIC presented worst page load time (PLT) results than TCP. We next present some of our interpretations of these results.
Comparing Kadish, TNM, and the modified Dulguerov staging systems for esthesioneuroblastoma

Esthesioneuroblastoma (ENB) is a rare neuroendocrine tumor. The purpose of this study was to compare the Kadish, tumor‐node‐metastasis (TNM), and Dulguerov's modified TNM staging in order to determine the impact of the stage on primary surgical treatment selection, margin status, and survival.
Quantitative imaging biomarkers alliance (QIBA) recommendations for improved precision of DWI and DCE‐MRI derived biomarkers in multicenter oncology trials

Physiological properties of tumors can be measured both in vivo and noninvasively by diffusion‐weighted imaging and dynamic contrast‐enhanced magnetic resonance imaging. Although these techniques have been used for more than two decades to study tumor diffusion, perfusion, and/or permeability, the methods and studies on how to reduce measurement error and bias in the derived imaging metrics is still lacking in the literature. This is of paramount importance because the objective is to translate these quantitative imaging biomarkers (QIBs) into clinical trials, and ultimately in clinical practice. Standardization of the image acquisition using appropriate phantoms is the first step from a technical performance standpoint. The next step is to assess whether the imaging metrics have clinical value and meet the requirements for being a QIB as defined by the Radiological Society of North America's Quantitative Imaging Biomarkers Alliance (QIBA). The goal and mission of QIBA and the National Cancer Institute Quantitative Imaging Network (QIN) initiatives are to provide technical performance standards (QIBA profiles) and QIN tools for producing reliable QIBs for use in the clinical imaging community. Some of QIBA's development of quantitative diffusion‐weighted imaging and dynamic contrast‐enhanced QIB profiles has been hampered by the lack of literature for repeatability and reproducibility of the derived QIBs. The available research on this topic is scant and is not in sync with improvements or upgrades in MRI technology over the years. This review focuses on the need for QIBs in oncology applications and emphasizes the importance of the assessment of their reproducibility and repeatability.
Fine-grained Classification using Heterogeneous Web Data and Auxiliary Categories

Fine-grained classification remains a very challenging problem, because of the absence of well-labeled training data caused by the high cost of annotating a large number of fine-grained categories. In the extreme case, given a set of test categories without any well-labeled training data, the majority of existing works can be grouped into the following two research directions: 1) crawl noisy labeled web data for the test categories as training data, which is dubbed as webly supervised learning; 2) transfer the knowledge from auxiliary categories with well-labeled training data to the test categories, which corresponds to zero-shot learning setting. Nevertheless, the above two research directions still have critical issues to be addressed. For the first direction, web data have noisy labels and considerably different data distribution from test data. For the second direction, zero-shot learning is struggling to achieve compelling results compared with conventional supervised learning. The issues of the above two directions motivate us to develop a novel approach which can jointly exploit both noisy web training data from test categories and well-labeled training data from auxiliary categories. In particular, on one hand, we crawl web data for test categories as noisy training data. On the other hand, we transfer the knowledge from auxiliary categories with well-labeled training data to test categories by virtue of free semantic information (e.g., word vector) of all categories. Moreover, given the fact that web data are generally associated with additional textual information (e.g., title and tag), we extend our method by using the surrounding textual information of web data as privileged information. Extensive experiments show the effectiveness of our proposed methods.
Prefrontal transcranial direct current stimulation (tDCS) enhances behavioral and EEG markers of proactive control

ABSTRACT This study examined the effects of stimulation targeting dorsolateral prefrontal cortex (DLPFC) on behavioral and neural oscillatory markers of proactive cognitive control in healthy adults. We hypothesized that active stimulation targeting the DLPFC would enhance proactive control compared to sham, leading to changes in the pattern of error rates and gamma-band power on the Dot Pattern Expectancy (DPX) task. We recorded EEG while participants completed the DPX, after receiving either 20 minutes of active DLPFC stimulation at 2 mA or sham stimulation in a counterbalanced within-participants design. The results showed significant tDCS-induced changes in the pattern of error rates on the DPX task indicative of enhanced proactive control, as well as predicted increases in gamma power associated with the engagement of proactive control. These results provide support for the role of DLPFC-mediated gamma activity in proactive cognitive control, and further, indicate that proactive control can be enhanced with non-invasive neurostimulation.
Reliability and accuracy of delirium assessments among investigators at multiple international centres

Introduction Delirium is a common, serious postoperative complication. For clinical studies to generate valid findings, delirium assessments must be standardised and administered accurately by independent researchers. The Confusion Assessment Method (CAM) is a widely used delirium assessment tool. The objective was to determine whether implementing a standardised CAM training protocol for researchers at multiple international sites yields reliable inter-rater assessment and accurate delirium diagnosis. Methods Patients consented to video recordings of CAM delirium assessments for research purposes. Raters underwent structured training in CAM administration. Training entailed didactic education, role-playing with intensive feedback, apprenticeship with experienced researchers and group discussions of complex cases. Raters independently viewed and scored nine video-recorded CAM interviews. Inter-rater reliability was determined using Fleiss kappa. Accuracy was judged by comparing raters’ scores with those of an expert delirium researcher. Results Twenty-seven raters from eight international research centres completed the study and achieved almost perfect agreement for overall delirium diagnosis, kappa=0.88 (95% CI 0.85 to 0.92). Agreement of the four core CAM features ranged from fair to substantial. The sensitivity and specificity for identifying delirium were 72% (95% CI 60% to 81%) and 99% (95% CI 96% to 100%), considering an expert rater’s scores as the reference standard (delirious, n=3; non-delirious, n=6). Delirium severity ratings were tightly clustered, with most scores within 5% of the median. Conclusion Our results demonstrate that, with appropriate training and ongoing scoring discussions, researchers at multiple sites can reliably detect delirium in postsurgical patients. These results support the premise that methodologically rigorous multi-centre studies can yield standardised and accurate determinations of delirium.
Leisure time and family functioning in families living with autism spectrum disorder

Family leisure can provide opportunities for both enjoyment and family growth. However, families of children with autism spectrum disorder experience multiple barriers to engaging in satisfying family leisure activities. This study surveyed parents of children with autism spectrum disorder (n = 112) and parents of children with typical development (n = 123) to examine relationships among family leisure involvement, leisure satisfaction, family functioning, and satisfaction with family life. Parents of children with autism spectrum disorder reported a similar amount of leisure involvement as families of typically developing children. However, they reported lower leisure satisfaction, poorer family functioning, and less satisfaction with family life. Mediation models suggested that low leisure satisfaction was related to less effective family communication, which in turn led to poorer family functioning and less satisfaction with family life. Amount of time spent in leisure made relatively small contributions to predicting other family variables. These results suggest that leisure-focused interventions for families of children with autism spectrum disorder should focus on improving quality, rather than quantity, of family leisure time.
A New Formula for Predicting the Fraction of Delivered Oxygen During Low-Flow Oxygen Therapy

BACKGROUND: During O2 therapy at low flow in patients who breathe spontaneously, the fraction of delivered O2 (FDO2) is unknown. In recent years, FDO2 prediction formulas have been proposed. However, they do not take into account the effect of inspiratory flow (V̇I) on the FDO2. The aim of this study was to validate a new FDO2 prediction formula, which takes into account the V̇I and compares it with other FDO2 prediction formulas. METHODS: During a bench study, spontaneous breathing was generated with a mechanical test lung connected to a mechanical ventilator set to volume control mode. O2 flow from a wall-mounted tube was delivered through a heat-and-moisture exchanger filter. A flow sensor recorded each breath of the V̇I in ambient temperature and barometric pressure conditions. Three parameters [O2 flow at 2, 3, 4, 5, 6 L/min; minute ventilation at 5, 10, 15, 20 L/min; and ratio of the inspiratory time (TI) to the total breathing cycle time (Ttot) (TI/Ttot) of 0.33 (TI/Ttot value) and 0.50 (TI/Ttot value)] were modified to generate many ventilatory patterns. An O2 analyzer continuously examined the FDO2. RESULTS: When the O2 flow and/or TI/Ttot increased, the FDO2 increased. When the minute ventilation increased, the FDO2 decreased. The results of the Bland-Altman method for the FDO2, calculated by using our mathematical model and the measured FDO2, showed that the mean ± SD bias value was equal to 1.49 ± 0.84%, and the limits of agreement ranged from −0.17% to 3.14%. The intraclass correlation coefficients were 0.991 for TI/Ttot = 0.33 and 0.994 for TI/Ttot = 0.50, and the coefficient of variation was 2.1% for TI/Ttot = 0.33 and 1.3% for TI/Ttot = 0.50. The results of the Bland-Altman method for the FDO2 calculated by using the Shapiro formula and the FDO2 measured on the bench indicated that the bias value was 0.075 ± 8.66% and the limits of agreement ranged from −16.89% to 17.04%. For the Vincent formula, the bias value was 3.08% ± 8.56% and the limits of agreement ranged from −13.69% to 19.84%. CONCLUSIONS: The V̇I has a major impact on FDO2 during O2 therapy at low flow. FDO2 comparisons between frequently used prediction formulas and FDO2 measured on the bench indicated greater differences. Uncritical use of these formulas should be used cautiously to predict FDO2. In this study, our prediction formula indicated a good accuracy for predicting FDO2 during supplemental oxygenation through a heat-and-moisture exchanger in patients who breathe spontaneously.
Gli2 modulates cell cycle re-entry through autophagy-mediated regulation of the length of primary cilia

ABSTRACT The primary cilium is a tiny cell protrusion known to transduce key extracellular signals, including those of the sonic hedgehog pathway, which activates Gli transcription factors for various cellular functions. To understand the significance of the Gli2 transcription factor in fibroblasts, we establish a Gli2-knockout NIH3T3 cell line by CRISPR/Cas9 technology. Surprisingly, NIH3T3 fibroblasts lacking Gli2 expression through gene knockout or RNA interference possess longer primary cilia after stimulation of ciliogenesis by serum starvation. This lengthening of primary cilia is associated with enhanced autophagy-mediated Ofd1 degradation, and can be reversed by pharmacological and genetic inhibition of autophagy. Meanwhile, flow cytometry reveals that Gli2−/− NIH3T3 fibroblasts exhibit a delay in cell cycle re-entry after serum re-stimulation. Ablation of their primary cilia through Kif3a knockdown rescues the delay in cell cycle re-entry. These results suggest that Gli2 plays an unexpected role in cell cycle re-entry through an autophagy-mediated regulation on ciliary length in fibroblasts. Summary: Gli2 knockout in fibroblasts lengthens primary cilia through enhanced autophagy-mediated Ofd1 degradation. This ciliary lengthening delays cell cycle re-entry – an unexpected mechanism for cell cycle regulation.