Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional and Morphological Characterization of Small and Large Steroidogenic Luteal Cells From Domestic Cats Before and During Culture

The current study aimed to isolate, culture and characterize small (SLC) and large (LLC) steroidogenic cells from the corpora lutea (CL) of non-pregnant domestic cats. Isolation of feline SLC was based on an enzymatic digestion of luteal tissue, whereas LLC were obtained by mechanical disruption of CL. To assess function of both cell types, progesterone secretion and mRNA expression of selected genes involved in steroid and prostaglandin synthesis were measured, as well as relative transcript abundance of hormone receptors and anti-oxidative enzymes, before and during culture. The cells were cultured for 3 or 5 days without gonadotropins. Isolated feline SLC and LLC had different sizes (12 ± 3 μm vs. 34 ± 5 μm, respectively), morphologies (amount of lipid droplets) and behaved differently in culture. SLC attached and proliferated or spread quickly, but lost their steroidogenic function during culture (significant decrease in progesterone secretion and expression of steroidogenic genes). The expression of receptors for gonadotropins and prolactin also decreased. Prostaglandin synthase (PTGS2) decreased steadily over time, whereas mRNA expression of PGE2 synthase (PGES) increased. The gene expression of anti-oxidative enzyme glutathione peroxidase 4 (GPX4), also increased during culture, but not of superoxide dismutase 1 (SOD1). In comparison to SLC, LLC did not attach to culture plates, secreted more progesterone per inoculated cells and maintained steroidogenic function during culture. Expression of prostaglandin synthases (PTGS2 and PGES) was almost non-detectable. The gene expression of hormone receptors for prostaglandin F2 alpha (PTGFR), gonadotropins (LHCHR and FSHR), and prolactin (PRLR), as well as of anti-oxidative enzymes (GPX4, SOD1), increased over time. To conclude, we successfully isolated and cultured different types of feline steroidogenic luteal cells and comprehensively characterized both isolated cell types. This knowledge can be used to better understand the CL lifecycle in felines more broadly, and the established cell cultures will provide a foundation for future studies on luteolytic and luteotrophic factors in the domestic cat, and for comparison with other feline species, particularly lynx.
Clinical Risk Score for Predicting Recurrence Following a Cerebral Ischemic Event

Introduction: Recurrent stroke has a higher rate of death and disability. A number of risk scores have been developed to predict short-term and long-term risk of stroke following an initial episode of stroke or transient ischemic attack (TIA) with limited clinical utilities. In this paper, we review different risk score models and discuss their validity and clinical utilities. Methods: The PubMed bibliographic database was searched for original research articles on the various risk scores for risk of stroke following an initial episode of stroke or TIA. The validation of the models was evaluated by examining the internal and external validation process as well as statistical methodology, the study power, as well as the accuracy and metrics such as sensitivity and specificity. Results: Different risk score models have been derived from different study populations. Validation studies for these risk scores have produced conflicting results. Currently, ABCD2 score with diffusion weighted imaging (DWI) and Recurrence Risk Estimator at 90 days (RRE-90) are the two acceptable models for short-term risk prediction whereas Essen Stroke Risk Score (ESRS) and Stroke Prognosis Instrument-II (SPI-II) can be useful for prediction of long-term risk. Conclusion: The clinical risk scores that currently exist for predicting short-term and long-term risk of recurrent cerebral ischemia are limited in their performance and clinical utilities. There is a need for a better predictive tool which can overcome the limitations of current predictive models. Application of machine learning methods in combination with electronic health records may provide platform for development of new-generation predictive tools.
Environmental and Benthic Community Patterns of the Shallow Hydrothermal Area of Secca Delle Fumose (Baia, Naples, Italy)

The occurrence of hydrothermal vent ecosystems at Secca delle Fumose, Pozzuoli Bay (Gulf of Naples), represented an opportunity to study the benthic assemblages under the thermal stress of hydrothermal emissions in a very shallow environment (9 – 14 m water depth). In autumn 2016, the macrobenthic community was sampled by scuba divers at four sites located in the Baia Underwater Archaeological Park. Two sites were characterized by vent emissions (one with white bacterial mat scattered on the bottom and one with a yellow substrate around a geyser opening) and two at about 100 m away, used as control. Sediment and interstitial water environmental variables were measured to determine their influence on the structure of macrobenthic assemblages. A total of 1,954 macrofaunal individuals was found, characterized by great differences in abundance and species richness among sites. This pattern was correlated to the dominance of a particular set of variables that drastically change in a very small spatial scale, from one site to another. The control sites, characterized by the highest percentage of gravel in the sediments (19.67±2.6%) and normal level of major ions such as Ca2+, K+ and Mg2+ in the interstitial waters, showed the highest values of sinecological indices. The “white” hydrothermal site exhibited the lowest species richness, abundance and species diversity, influenced by low pH values (~7.6), high temperatures (~37.53°C) and by the highest total organic carbon content (TOC 34.78%) in the sediment. The “yellow” hydrothermal site, with sediment TOC equal to 30.03% and interstitial sulphide ions measuring 130.58 ppm, showed higher values of sinecological indices than those recorded at the “white” site. Therefore, taxonomic analysis revealed a high turnover between control and vents sites. This highlights the preference for hydrothermal vents by a few resistant species, such as the gastropod Tritia cuvierii and the polychaete Capitella capitata, confirming the role of the latter species as opportunistic in extreme environments like Secca delle Fumose.
Differences in Semantic Memory Encoding Strategies in Young, Healthy Old and MCI Patients

Associative processes, such as the encoding of associations between words in a list, can enhance episodic memory performance and are thought to deteriorate with age. Here, we examine the nature of age-related deficits in the encoding of associations, by using a free recall paradigm with visual arrays of objects. Fifty-five participants (26 young students; 20 cognitive healthy older adults; nine patients with Mild Cognitive Impairment, MCI) were shown multiple slides (experimental trials), each containing an array of nine common objects for recall. Most of the arrays contained three objects from three semantic categories, each. In the remaining arrays, the nine objects were unrelated. Eye fixations were also monitored during the viewing of the arrays, in a subset of the participants. While for young participants the immediate recall was higher for the semantically related arrays, this effect was diminished in healthy elderly and totally absent in MCI patients. Furthermore, only in the young group did the sequence of eye fixations show a semantic scanning pattern during encoding, even when the related objects were non- adjacent in the array. Healthy elderly and MCI patients were not influenced by the semantic relatedness of items during the array encoding, to the same extent as young subjects, as observed by a lack of (or reduced) semantic scanning. The results support a version of the encoding of the association aging-deficit hypothesis.
The influence of exergaming on the functional fitness in overweight and obese children : : physical activity, health and wellness

The rapidly growing rate of childhood obesity makes it imperative to develop an effective intervention programme for obese children. This study was designed to improve overweight and obese children's functional fitness through exergaming. The study consisted of a 6-week intervention on the Nintendo Wii. Participants were aged between 9-12 years old. Subjects were assigned to three groups according to location: experimental group (n=11), control group A (n=10) and control group B (n=10). The experimental group participated in the exergaming intervention programme, control group A had access to traditional video games and control group B continued with their everyday life activities with no intervention. Bruininks-Oseretsky protocols were used to assess the functional fitness of the subjects. Statistical calculations revealed that the experimental group showed significant improvement in their level of functional fitness (p<0.05). Coordination, reaction time, speed and agility increased by magnitudes of 28%, 94% and 37% respectively. Both the control groups displayed marginal increases in their functional fitness that were not significant. These results support the concept that exergaming can be used as an alternative means to improve the level of functional fitness as well as promoting physical activity in children.
Siglec-G Deficiency Ameliorates Hyper-Inflammation and Immune Collapse in Sepsis via Regulating Src Activation

Hyper-inflammation during acute phase and sequential hypo-inflammation during immunosuppressive phase in macrophages/monocytes lead to multiorgan failure syndrome and immune collapse of sepsis, in which toll-like receptor (TLR)-triggered inflammatory responses play a major role. Here, we reported that Siglecg deficiency attenuated TLR4-triggered pro-inflammatory cytokine production and increased anti-inflammatory cytokine [interleukin-10 [IL-10]] production in vivo and in vitro at both acute and immunosuppressive phases. Siglecg deficiency also protected mice from lipopolysaccharide (LPS)-induced sepsis with less inflammation in the lung and less tissue destruction in the spleen. Siglec-G inhibited proto-oncogene tyrosine-protein kinase Src (Src) activation via recruiting and activating tyrosine phosphatase Src homology region 2 domain-containing phosphatase-1 (SHP1) through immunoreceptor tyrosine-based inhibitory motif (ITIM) domain. Src could inhibit TLR4-induced inflammatory cytokines and promote anti-inflammatory cytokine IL-10. Mechanical investigation showed that Src could interact with and phosphorylate STAT3. Src could also promote HIF1α degradation through activating GSK3β. Our study reveals that Siglec-G orchestrates TLR-induced inflammation, which outlines that blocking Siglec-G or activating Src may be a promising strategy for both acute and chronic inflammatory diseases.
Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field

The pulsed electric field (PEF) technology has been widely applied to inactivate pathogenic bacteria in food products. Though irreversible pore formation and membrane disruption is considered to be the main contributing factor to PEF’s sterilizing effects, the exact molecular mechanisms remain poorly understood. In this study, by using mass spectrometry (MS)-based label-free quantitative proteomic analysis, we compared the protein profiles of PEF-treated and untreated Escherichia coli. We identified a total of 175 differentially expressed proteins, including 52 candidates that were only detected in at least two of the three samples in one experiment group but not in the other group. Functional analysis revealed that the differential proteins were primarily involved in the regulation of cell membrane composition and integrity, stress response, as well as various metabolic processes. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was conducted on the genes of selected differential proteins at varying PEF intensities, which were known to result in different cell killing levels. The qRT-PCR data confirmed that the proteomic results could be reliably used for further data interpretation, and that the changes in the expression levels of the differential candidates were, to a large extent, caused directly by the PEF treatment. The findings of the current study offered valuable insight into PEF-induced cell inactivation.
Design, fabrication, and characterization of a compact magnetron sputtering system for micro/nano fabrication

Department of Electrical Engineering and C Science January 15, 2019 Signature redacted C ertified b y . Martin A. Schmidt Institute Provost and Professor of Electrical Engineering and Computer Science Thesis Supervisor A ccepted by. Signature redacted U U Leslie A. Kolodziejski Professor of Electrical Engineering and Computer Science Chair, Committee for Graduate Students MASSACHUSETTS INSTITUTE OF TECHNOLOGY
The Dietary Inflammatory Index® and Alternative Healthy Eating Index 2010 in relation to leucocyte telomere length in postmenopausal women: a cross-sectional study

Abstract Telomeres are nucleoprotein complexes that form the ends of eukaryotic chromosomes where they protect DNA from genomic instability, prevent end-to-end fusion and limit cellular replicative capabilities. Increased telomere attrition rates, and relatively shorter telomere length, is associated with genomic instability and has been linked with several chronic diseases, malignancies and reduced longevity. Telomeric DNA is highly susceptible to oxidative damage and dietary habits may make an impact on telomere attrition rates through the mediation of oxidative stress and chronic inflammation. The aim of this study was to examine the association between leucocyte telomere length (LTL) with both the Dietary Inflammatory Index® 2014 (DII®) and the Alternative Healthy Eating Index 2010 (AHEI-2010). This is a cross-sectional analysis using baseline data from 263 postmenopausal women from the Alberta Physical Activity and Breast Cancer Prevention (ALPHA) Trial, in Calgary and Edmonton, Alberta, Canada. No statistically significant association was detected between LTL z-score and the AHEI-2010 (P = 0·20) or DII® (P = 0·91) in multivariable adjusted models. An exploratory analysis of AHEI-2010 and DII® parameters and LTL revealed anthocyanidin intake was associated with LTL (P < 0·01); however, this association was non-significant after a Bonferroni correction was applied (P = 0·27). No effect modification by age, smoking history, or recreational physical activity was detected for either relationship. Increased dietary antioxidant and decreased oxidant intake were not associated with LTL in this analysis.
Whole Genome Analysis of Selected Human Group A Rotavirus Strains Revealed Evolution of DS-1-Like Single- and Double-Gene Reassortant Rotavirus Strains in Pakistan During 2015–2016

Acute gastroenteritis due to group A rotaviruses (RVAs) is the leading cause of infant and childhood morbidity and mortality particularly in developing countries including Pakistan. In this study we have characterized the whole genomes of five RVA strains (PAK56, PAK419, PAK585, PAK622, and PAK663) using the Illumina HiSeq platform. The strains PAK56 and PAK622 exhibited a typical Wa-like genotype constellation (G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 and G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, respectively), whereas PAK419, PAK585, and PAK663 exhibited distinct DS-1-like genotype constellations (G3P[4]-I2-R2-C2-M2-A2-N2-T1-E2-H2, G1P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2, and G3P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2, respectively). Despite their DS-1-like genotype constellation, strain PAK585 possessed the typical Wa-like G1P[8] genotypes, whereas both PAK419 and PAK663 possessed the G3 genotype. In addition, PAK419 also possessed the Wa-like NSP3 genotype T1, suggesting that multiple reassortments have occurred. On Phylogenetic analysis, all of the gene segments of the five strains examined in this study were genetically related to globally circulating human G1, G2, G3, G6, G8, G9, and G12 strains. Interestingly, the NSP2 gene of strain PAK419 showed closest relationship with Indian bovine strain (India/HR/B91), suggesting the occurrence of reassortment between human and bovine RVA strains. Furthermore, strains PAK419, PAK585, and PAK663 were closely related to one another in most of their gene segments, indicating that these strains might have been derived from a common ancestor. To our knowledge this is the first whole genome-based molecular characterization of human rotavirus strains in Pakistan. The results of our study will enhance our existing knowledge on the diversity and evolutionary dynamics of novel RVA strains including DS-1-like intergenogroup reassortant strains spreading in Asian countries including Pakistan, in the pre-vaccine era. Therefore, continuous surveillance is recommended to monitor the evolution, spread and genetic stability of novel reassortant rotavirus strains derived from such events.
Accelerating the Merge Phase of Sort-Merge Join

We present an efficient, high-throughput and scalable hardware design for accelerating the merge phase of the sort-merge join operation. Sort-merge join is one of the fundamental join algorithms and among the most frequently executed operations in relational databases. It has been the focus of various recent pieces of research, each having different shortcomings and usually only focusing on the sort phase. In this paper, a new parallel sort-merge join architecture is developed, that provides data streaming functionality and high throughput. The key idea of the paper is the use of a novel design for a co-grouping engine, with which the input data are summarised on-the-fly. In this way, the operation is performed on streams of data, preserving the linear access pattern for faster data movement and also eliminates the need for a replay buffer/cache. In contrast to related work, our approach does not make assumptions about the value distribution of the input data and applies to any input size and width. We evaluate the design on a Zynq UltraScale+ based platform, and show that there is up to 3.1 times speedup over the host processor, even without accelerating the sort phase, while still taking into account the data transfers from and to main memory in Linux.
Oh, the Places You've Been! User Reactions to Longitudinal Transparency About Third-Party Web Tracking and Inferencing

Internet companies track users' online activity to make inferences about their interests, which are then used to target ads and personalize their web experience. Prior work has shown that existing privacy-protective tools give users only a limited understanding and incomplete picture of online tracking. We present Tracking Transparency, a privacy-preserving browser extension that visualizes examples of long-term, longitudinal information that third-party trackers could have inferred from users' browsing. The extension uses a client-side topic modeling algorithm to categorize pages that users visit and combines this with data about the web trackers encountered over time to create these visualizations. We conduct a longitudinal field study in which 425 participants use one of six variants of our extension for a week. We find that, after using the extension, participants have more accurate perceptions of the extent of tracking and also intend to take privacy-protecting actions.
Do Switches Dream of Machine Learning?: Toward In-Network Classification

Machine learning is currently driving a technological and societal revolution. While programmable switches have been proven to be useful for in-network computing, machine learning within programmable switches had little success so far. Not using network devices for machine learning has a high toll, given the known power efficiency and performance benefits of processing within the network. In this paper, we explore the potential use of commodity programmable switches for in-network classification, by mapping trained machine learning models to match-action pipelines. We introduce IIsy, a software and hardware based prototype of our approach, and discuss the suitability of mapping to different targets. Our solution can be generalized to additional machine learning algorithms, using the methods presented in this work.
A clinical comparative study on the efficacy of Apamarg Kshar Sutra and Seton therapy in the manazement of Bhagander W.S.R. to Fistula in Ano

Kshara Sutra is a unique and an established procedure for the management of Bhagandar in ayurveda. It has brought revolution in the Indian system of surgery. The aim of study was to compare Apamarg Kshar sutra therapy with Seton (linen thread) in the management of fistula in ano. Thirty patients with Low anal of fistula in ano were selected and divided into two groups of fifteen patients each. Group A was treated with Apamarg Kshar sutra and the Group B with the Seton (surgical linen thread number 20). All patients were treated on an outpatient’s basis in minor kshasutra OT in govt. ayurved college Raipur. Statistical analysis used Student’s t-test was applied for statistical analysis. Results the healing occurred in all the patients treated either with Apamarg Kshar Sutra or plain thread. The median healing time was 7 weeks for Kshar Sutra and 11 weeks with with Seton therapy. Recurrence rate was only 0.0 percent (0 patient) with the Kshar Sutra and 13.33 percent (4 patients) with Seton therapy. Conclusion The technique of Apamarg Kshar Sutra therapy is appropriate for healing of the fistulous tract with minimal complications. At the end of the study it was found that Apamarg kshar sutra is more competent and effective than Seton therapy in the management of Bhagandara. In kshara-sutra therapy the cutting and healing of fistulous track takes simultaneously.
The Perpetual Work Life of Crowdworkers

Crowdworkers regularly support their work with scripts, extensions, and software to enhance their productivity. Despite their evident significance, little is understood regarding how these tools affect crowdworkers' quality of life and work. In this study, we report findings from an interview study (N=21) aimed at exploring the tooling practices used by full-time crowdworkers on Amazon Mechanical Turk. Our interview data suggests that the tooling utilized by crowdworkers (1) strongly contributes to the fragmentation of microwork by enabling task switching and multitasking behavior; (2) promotes the fragmentation of crowdworkers' work-life boundaries by relying on tooling that encourages a 'work-anywhere' attitude; and (3) aids the fragmentation of social ties within worker communities through limited tooling access. Our findings have implications for building systems that unify crowdworkers' work practice in support of their productivity and well-being.
Patho-anatomical studies on poultry mortality with special reference to gastro-intestinal tract disorders.

To study etiopathology with special reference to gastro-intestinal tract disorders, data pertaining to mortality in poultry between January 2004 and December 2008 was collected and was eo-related with gastro-intestinal disorders. Analysis of the data revealed that mortality was maximum in adult age group (29.1 I%) and affections of digestive system accounted for highest mortality (30.88%). The main gastrointestinal affections were colibacillosis, enteritis, hepatitis, coccidiosis, fatty liver and enlargement of liver.