Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of SH2 and SH3 deletions on the functional activities of wild-type and transforming variants of c-Src

The amino-termina, noncatalytic half of Src contains two domains, designated the Src homology 2 (SH2) and Src homology 3 (SH3) domains, that are highly conserved among members of the Src family of tyrosine kinases. The SH2 domain (which can be further divided into the B and C homology boxes) and the SH3 domain (also referred to as the A box) are also found in several proteins otherwise unrelated to protein tyrosine kinases. It is believed that these domains are important for directing specific protein-protein interactions necessary for the proper functioning of Src. To determine the importance of the SH2 and SH3 domains in regulating the functions of c-Src, we evaluated mutants of c-Src lacking the A box (residues 88 to 137), the B box (residues 148 to 187) or the C box (residues 220 to 231). Each of these deletions caused a 14- to 30-fold increase in the in vitro level of kinase activity of c-Src. Chicken embryo fibroblasts expressing the deletion mutants displayed a transformed cell morphology, formed colonies in soft agar, and contained elevated levels of cellular phosphotyrosine-containing proteins. Src substrates p36, p85, p120, p125, the GTPase-activating protein (GAP), and several GAP-associated proteins were phosphorylated on tyrosine in cells expressing the A, B, or C box deletion mutant. p110 was highly phosphorylated in cells expressing the C box mutant, was weakly phosphorylated in cells expressing the B box mutant, and was not phosphorylated in cells expressing the A box mutant. Expression of the mutant proteins caused a reorganization of the actin cytoskeleton similar to that seen in v-Src-transformed cells. In addition, deletion of the A, B, or C box did not diminish the transforming or enzymatic activity of an activated variant of c-Src, E378G. These data indicate that deletion of the A, B, or C homology box causes an activation of the catalytic and transforming potential of c-Src and that while these mutations caused subtle differences in substrate phosphorylation, the homology boxes are not required for many of the phenotypic changes associated with transformation by Src.
The Haber Bosch–harmful algal bloom (HB–HAB) link

Large-scale commercialization of the Haber–Bosch (HB) process is resulting in intensification of nitrogen (N) fertilizer use worldwide. Globally N fertilizer use is far outpacing that of phosphorus (P) fertilizer. Much of the increase in N fertilizers is also now in the form of urea, a reduced form of N. Incorporation of these fertilizers into agricultural products is inefficient leading to significant environmental pollution and aquatic eutrophication. Of particular concern is the increased occurrence of harmful algal blooms (HABs) in waters receiving nutrient enriched runoff. Many phytoplankton causing HABs have physiological adaptive strategies that make them favored under conditions of elevated N : P conditions and supply of chemically reduced N (ammonium, urea). We propose that the HB-HAB link is a function of (1) the inefficiency of incorporation of N fertilizers in the food supply chain, the leakiness of the N cycle from crop to table, and the fate of lost N relative to P to the environment; and (2) adaptive physiology of many HABs to thrive in environments in which there is excess N relative to classic nutrient stoichiometric proportions and where chemically reduced forms of N dominate. The rate of HAB expansion is particularly pronounced in China where N fertilizer use has escalated very rapidly, where soil retention is declining, and where blooms have had large economic and ecological impacts. There, in addition to increased use of urea and high N : P based fertilizers overall, escalating aquaculture production adds to the availability of reduced forms of N, as does atmospheric deposition of ammonia. HABs in both freshwaters and marginal seas in China are highly related to these overall changing N loads and ratios. Without more aggressive N control the future outlook in terms of HABs is likely to include more events, more often, and they may also be more toxic.
Almost Envy-Freeness with General Valuations

The goal of fair division is to distribute resources among competing players in a "fair" way. Envy-freeness is the most extensively studied fairness notion in fair division. Envy-free allocations do not always exist with indivisible goods, motivating the study of relaxed versions of envy-freeness. We study the envy-freeness up to any good (EFX) property, which states that no player prefers the bundle of another player following the removal of any single good, and prove the first general results about this property. We use the leximin solution to show existence of EFX allocations in several contexts, sometimes in conjunction with Pareto optimality. For two players with valuations obeying a mild assumption, one of these results provides stronger guarantees than the currently deployed algorithm on Spliddit, a popular fair division website. Unfortunately, finding the leximin solution can require exponential time. We show that this is necessary by proving an exponential lower bound on the number of value queries needed to identify an EFX allocation, even for two players with identical valuations. We consider both additive and more general valuations, and our work suggests that there is a rich landscape of problems to explore in the fair division of indivisible goods with different classes of player valuations.
Value‐based segmentation of luxury consumption behavior

Following a broader perspective in exploring customer perceptions of and motives for purchasing luxury brands, it is not sufficient to explain the whole picture of luxury consumption in terms of socially oriented consumer motives and the desire to impress others. The main contribution here is to explore a multidimensional framework of luxury value as a general basis for identifying value-based consumer segments. The empirical results can be seen as a first step toward a better understanding of consumers' luxury value perceptions as based on social, individual, functional, and financial aspects. ©2009 Wiley Periodicals, Inc.
Community-based ecotourism: a collaborative partnerships perspective

Collaboration is a key principle in community-based tourism approaches in most developing countries. This paper assesses how community-based ecotourism is perceived in terms of community participation and empowerment. Data were collected through semi-structured interviews and secondary sources. Collaborative partnerships underpinnings are adopted to guide the research. Results suggest that participation in ecotourism brings mixed results on biodiversity conservation and community livelihoods due to the involvement of multiple stakeholders in the design, planning, and implementation of ecotourism projects. Due to the diversity of stakeholders, the empowerment of communities using ecotourism is complex. Nonetheless, while the study may be perceived as having attained mixed results, the case study offers a progressive example of how stakeholders approach to natural resource management are evolving. Ecotourism development in Botswana still needs improvement; more considerations have to be given to in situ settings. The Chobe Enclave Community Trust, a community living adjacent to Chobe National Park in Botswana, provides the context on which this study's discussion focuses.
Routes of Reuse of Second-hand Goods in Melbourne Households

Abstract While various Australian state governments have set targets for the reduction of waste to landfill, the emphasis to date has been placed mainly on the development of bulk materials recycling industries than on other strategies for waste reduction such as the reuse of second-hand goods. Many second-hand channels are informal and do not involve market transactions, making it difficult to collect information that could identify patterns. In this paper we report on the findings of a survey conducted with 306 Melbourne households about their practices of acquiring and disposing of used household goods. Our survey results confirm the importance of both formal and informal channels for the circulation of second-hand goods among Melbourne households. Socio-demographic characteristics such as household composition, employment status, education level and country of birth, along with infrastructure issues, such as dwelling types and residential tenures, are significant predictors of the use of various second-hand channels. A fuller understanding of the routes of reuse of second-hand goods will require grappling with complex intergenerational and gender effects, and large differences between types of dwellings, systems of infrastructure and living situations among Melbourne householders.
Weight minus extracellular fluid as metabolic reference standard in newborn baby

In 51 babies of differing size and gestational age, rates of oxygen consumption and corrected bromide space (as a measure of extracellular fluid) were measured. The results are used to examine the concept that weight minus extracellular fluid (ECF) is an appropriate metabolic reference standard in the newborn baby. When the whole group, which included large-for-dates and small-for-dates babies, is considered there is a systematic variation wherein the rate of oxygen consumption thus expressed varies with size. However, when appropriately grown babies only are considered, who varied in birthweight from 1210 g to 3820 g, rates of oxygen consumption thus expressed were constant. The implication is that when unusual rates of oxygen consumption per kilogram body weight are found, they should be interpreted bearing in mind the possibility of an unusual proportion of ECF in the baby. An incidental finding was that small-for-dates babies have a relatively large corrected bromide space.
MULTIPLE PLASMOID EJECTIONS AND ASSOCIATED HARD X-RAY BURSTS IN THE 2000 NOVEMBER 24 FLARE

The Soft X-ray Telescope (SXT) on board Yohkoh revealed that the ejection of X-ray emitting plasmoid is sometimes observed in a solar flare. It was found that the ejected plasmoid is strongly accelerated during a peak in the hard X-ray (HXR) emission of the flare. In this paper, we present an examination of the GOES X 2.3 class flare that occurred at 14:51 UT on 2000 November 24. In the SXT images, we found “multiple” plasmoid ejections with velocities in the range of 250–1500 km s−1, which showed blob-like or loop-like structures. Furthermore, we also found that each plasmoid ejection is associated with an impulsive burst of HXR emission. Although some correlation between plasmoid ejection and HXR emission has been discussed previously, our observation shows similar behavior for multiple plasmoid ejection such that each plasmoid ejection occurs during the strong energy release of the solar flare. As a result of temperature-emission measure analysis of such plasmoids, it was revealed that the apparent velocities and kinetic energies of the plasmoid ejections show a correlation with the peak intensities in the HXR emissions.
Transient fault tolerance in digital systems

It is hard to shield systems effectively from transient faults (fault avoidance techniques). So some other means must be employed to assure appropriate levels of transient fault tolerance (insensitivity to transient faults). They are based on fault-masking and fault recovery ideas. Having analyzed this problem, the author identifies critical design points and outlines some practical solutions that refer to efficient on-line detectors (detecting errors during the system operation) and error handling procedures. This framework provides a basis for understanding transient fault problems in digital systems. It can be helpful in selecting optimum techniques to mask or eliminate transient fault effects in developed systems. <<ETX>>
Treatment outcomes after endopyelotomy performed with or without simultaneous nephrolithotomy: 10-year experience.

INTRODUCTION The incidence of renal calculi associated with ureteropelvic junction obstruction (UPJO) has been reported to be as high as 20%. Although it has been suggested that simultaneous stone removal be performed with endopyelotomy (EP) for patients with UPJO, no crossing vessel, and renal calculi, there are no large series to date reporting in a rigorous fashion the success rate for resolution of the UPJO. This study intends to determine if stone extraction performed with EP increases failure rate. MATERIALS AND METHODS A retrospective review was performed for all patients who underwent EP by a single surgeon between August 1996 and November 2006. One hundred forty-six procedures for UPJO were done in 139 patients. Success rate was compared in 105 cases (72%) of isolated UPJO and 41 (28%) cases with UPJO and ipsilateral calculi. Determination of overall success required subjective improvement and objective data based on intravenous pyelogram, computed tomography, and/or nuclear scintigraphy. RESULTS Overall success rate was 71% in patients undergoing EP only and 90% in 41 patients who had simultaneous nephrolithotomy (p = 0.04). The odds ratio of failure after EP was 2.9 for severe/massive preoperative hydronephrosis. When considered alone and after adjusting for the severity of preoperative hydronephrosis and/or renal function, simultaneous nephrolithotomy did not increase the EP failure rate. CONCLUSION This study demonstrates that UPJO resolves at an equal rate after EP performed with or without ipsilateral renal calculi. Patients with UPJO and renal calculi should undergo stone extraction and EP in the same setting with the expectation of excellent results.
Globalization and Domestic Trade Policy Preferences: Foreign Frames and Mass Support for Agriculture Subsidies

Reforming agriculture trade policy is key to breaking the deadlock in multilateral trade negotiations. While existing studies have focused on institutions and interest group barriers to agriculture trade reform in developed countries, most have failed to recognize the broad support for agriculture protection among developed countries. In this article we examine one of the drivers of this support: the ability of politicians to frame their own agriculture policies as less generous relative to those of other countries. Drawing on existing literature on heuristics, we argue that voters are malleable to politicians’ comparative framing of agriculture policies. Using an original survey experiment in the United States, we find that framing US agriculture as less generous than other countries generates an additional 12% of respondents supporting increased farm payments to US farmers. These results speak to the difficulty in reforming agriculture and more broadly about the lack of public support for unilateral trade liberalization.
Analyzing and Forecasting Volatility Spillovers and Asymmetries in Major Crude Oil Spot, Forward and Futures Markets

Crude oil price volatility has been analyzed extensively for organized spot, forward and futures markets for well over a decade, and is crucial for forecasting volatility and Value-at-Risk (VaR). There are four major benchmarks in the international oil market, namely West Texas Intermediate (USA), Brent (North Sea), Dubai/Oman (Middle East), and Tapis (Asia-Pacific), which are likely to be highly correlated. This paper analyses the volatility spillover and asymmetric effects across and within the four markets, using three multivariate GARCH models, namely the constant conditional correlation (CCC), vector ARMA-GARCH (VARMA-GARCH) and vector ARMA-asymmetric GARCH (VARMA-AGARCH) models. A rolling window approach is used to forecast the 1-day ahead conditional correlations. The paper presents evidence of volatility spillovers and asymmetric effects on the conditional variances for most pairs of series. In addition, the forecast conditional correlations between pairs of crude oil returns have both positive and negative trends. Moreover, the optimal hedge ratios and optimal portfolio weights of crude oil across different assets and market portfolios are evaluated in order to provide important policy implications for risk management in crude oil markets.
Bladder and bowel symptoms in cervical and endometrial cancer survivors

Previous studies likely underestimate the prevalence of bowel and bladder symptoms in gynecologic cancer survivors. We sought to estimate the prevalence of these symptoms in cervical and endometrial cancer survivors who had completed treatment 1 year previously compared with non‐cancer controls, and to examine factors associated with more severe symptoms in survivors.
Alkali Burn to the Eye: Protection Using TNF-&agr; Inhibition

Purpose: The aim of this study was to evaluate early retinal damage after induction of ocular surface alkali burns and the protective effects of tumor necrosis factor alpha (TNF-&agr;) blockade. Methods: Alkali injury was induced in mouse corneas by using 1 N NaOH. Retinal damage was assessed using a terminal deoxynucleotidyl transferase 2′-deoxyuridine 5-triphosphate nick end labeling (TUNEL) assay, 15 minutes to 14 days postburn. Immune cell infiltration was assessed by CD45 immunolocalization. Retinal cytokines were quantified using the enzyme-linked immunosorbent assay for interleukin (IL)1&bgr;, IL2, IL6, TNF-&agr;, CCL5, and macrophage inflammatory protein-1&agr;. Protection against retinal damage was attempted with a single dose of either anti–TNF-&agr; antibody (infliximab, 6.25 mg/kg) or control immunoglobulin G (IgG), administered intraperitoneally 15 minutes after the burn was inflicted. Corneal injury was evaluated by using TUNEL and CD45 immunolocalization and by quantifying corneal neovascularization. Results: There was significant damage to the retina within 24 hours of the corneal burn being inflicted. TUNEL+ labeling was present in 80% of the retinal ganglion cells, including a few CD45+ cells. There was a 10-fold increase in the retinal inflammatory cytokines in the study groups compared with that in controls. A single intraperitoneal dose of anti–TNF-&agr; antibody, administered 15 minutes after the burn, markedly reduced retinal TUNEL+, CD45+ labeling, and inflammatory cytokine expression, compared with that in the controls. Additionally, TNF-&agr; blockade caused a marked reduction in corneal neovascularization, and in cornea TUNEL and CD45 labeling, 5 days after the burn. Conclusions: This study shows that alkali corneal burns can induce significant retinal damage within 24 hours. A single dose of anti–TNF-&agr; antibody, administered 15 minutes after inflicting the burn, provides significant retinal and corneal protection. This could lead to the discovery of novel therapies for patients with alkali injuries.
Governing the smart city: a review of the literature on smart urban governance

Academic attention to smart cities and their governance is growing rapidly, but the fragmentation in approaches makes for a confusing debate. This article brings some structure to the debate by analyzing a corpus of 51 publications and mapping their variation. The analysis shows that publications differ in their emphasis on (1) smart technology, smart people or smart collaboration as the defining features of smart cities, (2) a transformative or incremental perspective on changes in urban governance, (3) better outcomes or a more open process as the legitimacy claim for smart city governance. We argue for a comprehensive perspective: smart city governance is about crafting new forms of human collaboration through the use of ICTs to obtain better outcomes and more open governance processes. Research into smart city governance could benefit from previous studies into success and failure factors for e-government and build upon sophisticated theories of socio-technical change. This article highlights that smart city governance is not a technological issue: we should study smart city governance as a complex process of institutional change and acknowledge the political nature of appealing visions of socio-technical governance. Points for practitioners The study provides practitioners with an in-depth understanding of current debates about smart city governance. The article highlights that governing a smart city is about crafting new forms of human collaboration through the use of information and communication technologies. City managers should realize that technology by itself will not make a city smarter: building a smart city requires a political understanding of technology, a process approach to manage the emerging smart city and a focus on both economic gains and other public values.
Integrating Multiple Intelligences in Teaching English as a Foreign Language - Seeu Experiences and Practices

Abstract The purpose of this article is to discuss the Theory of Multiple Intelligences and to answer the question of appropriateness of the MI theory in the field of foreign language teaching. The paper also exemplifies the adaptation of the theory in the context of teaching English as a foreign language and foreign language teaching and learning in general by describing a set of activities and a lesson plan using the MI approach. The article reviews the types of intelligences described and defined by Howard Gardner and authors who followed and revised the theory in terms of language teaching. In addition, the article discusses the different modes of application of the theory of Multiple Intelligences in language teaching with young adults and adult learners of foreign languages. The article proceeds by grouping and listing possible activities and tasks which are appropriate for language learners with different sets of abilities or intelligences. In addition, the article provides a brief summary of the potential issues recommendations and conclusions regarding the implementation of theory of Multiple Intelligences and provides a sample lesson plan which provides activities and language learning tasks for the majority of intelligences defined
Emergency Use Only: Understanding and reducing the use of food banks in the UK

The use of emergency food aid in the UK, particularly in the form of food banks, has dramatically increased over the last decade. Research was jointly conducted by Oxfam, Child Poverty Action Group (CPAG), the Church of England and The Trussell Trust to examine why people are turning to food banks, how food bank use fits with their wider coping strategies, and what might be done to reduce the need that leads to food bank use. Interviews with clients at seven food banks across the UK revealed that the acute crisis that leads people to turn to food banks is often set against a background of complex, difficult lives. Experiences included ill health, bereavement, relationship breakdown, heavy caring responsibilities or job loss, as well as constantly low income. The report shows that action is needed to ensure that the safety net provided by the social security system is vital. It can help prevent life shocks becoming crises, and offer vital protection for vulnerable people. This report points to practical, measured changes in policy and practice that will help to reduce the need for food banks, and ensure vital support for people in times of crisis.