Miracle-dz/UniFAR-FTUs huggingface dataset:
Development and Validation of a Life Satisfaction Scale for Chinese Elders

This study reports the development and validation of a culturally sensitive, domain-specific measure of life satisfaction for Chinese Elders — The Life Satisfaction Scale — Chinese (LSS-C). The LSS-C was administered to 1,502 randomly-selected older Chinese persons in three newly developed towns in Mainland China. Confirmatory factor analysis supported a conceptually meaningful two-factor model—one of instrumental needs of daily life and one of social and relational needs. Criterion-related validity was satisfactory when positively correlated with a single-item global life satisfaction measure and the Life Satisfaction Index A, and negatively correlated with the Center for Epidemiological Studies Depression Scale. The reliability of the eight-item scale was satisfactory, with the internal consistency coefficient equal to . 78 and the split half coefficient equal to . 81. LSS-C was direct and easy to administer, and it is recommended for use on Chinese elders.
CYP19 gene variants affect the assisted reproduction outcome of women with polycystic ovary syndrome

Abstract Objective: Cytochrome P450 aromatase catalyzes the irreversible transformation of androgens into estrogens. The association of CYP19(TTTA)n polymorphism with the hormonal profile and the assisted reproduction outcome of women with polycystic ovary syndrome (PCOS) was explored. Methods: One hundred and thirty-two women with PCOS and 200 with male-factor infertility, as controls, participated in the current study. The CYP19(TTTA)n polymorphism was genotyped, while the hormonal profile was determined at the third day of the menstrual cycle. During oocyte retrieval, the follicular size, the follicle and oocyte numbers were recorded. Results: Genotype analysis revealed 6 CYP19(TTTA)n alleles with 7–12 repeats. In PCOS women, the CYP19(TTTA)7 allele presence was associated with lower serum E2 levels at the third day of the menstrual cycle (p < 0.009), lower large follicle (p < 0.02) and total oocyte numbers (p = 0.006), but with significantly higher pregnancy rates after assisted reproduction (p < 0.004). Conclusions: Potential associations of the CYP19(TTTA)7 allele with ovarian response to standard gonadotrophin stimulation and with assisted reproduction outcome were found in PCOS women, probably due to androgen/estrogen ratio alterations.
Social democratic austerity: the conditional role of agenda dynamics and issue ownership

ABSTRACT Over the past 30 years, left-wing parties have increasingly abandoned the interests of their core constituency to embark on a right-wing journey of fiscal austerity. I argue that the changing partisan pattern in fiscal policy can in part be explained by analysing social democrats’ need to signal fiscal competence. Drawing on issue ownership theory, my starting point is that voters perceive the Right as better able to balance the budgets than the Left. This leads left-wing incumbents to compensate for their bad fiscal reputation when concerns about balanced budgets are salient in the political system. They do so by reducing spending and budget deficits as much or even more than right-wing governments. In this way, an asymmetric fiscal reputation makes partisan differences in fiscal policy disappear. Support for my claims is found estimating a set of error correction models with data from 21 Organization for Economic Co-operation and Development countries between 1980 and 2006.
Interdisciplinary Research Experiences For Undergraduates: Two Mixed-Methods Studies

Despite the demand for a diverse STEM-educated population and workforce, college students have consistently turned away from these disciplines in large numbers, creating a persistent problem that many are trying to address. The aim of the National Science Foundation’s Research Experiences for Undergraduates (REU) program is to inspire, attract, and retain STEM majors. Funding supports undergraduate STEM students’ engagement in real-world research alongside STEM mentors. As colleges and universities compete for funding for REUs, it is important to understand the mechanisms within summer research programs that resonate most deeply with undergraduate STEM researchers. While many studies reveal strong correlations between research experiences and STEM aspirations, less is known about the mechanisms within REU programs that support these gains. My research used quantitative and qualitative self-reported data from 20 REU students, 18 of whom were underrepresented minorities in STEM. Over two summers, these students, in cohorts of ten, came to the University of Vermont to participate in a team-oriented, 10-week REU: Interdisciplinary Research on Human Impacts in the Lake Champlain Ecosystem. Two mixed-methods studies, guided by the frameworks of the theory of possible selves, theory of self-efficacy for research, and social cognitive career theory, revealed four important program mechanisms that gave rise to gains in research skills, confidence and self-efficacy for research, and STEM career aspirations, particularly for individuals from underrepresented minority groups in STEM. Findings suggest that the program fostered student capacity building within a safe, inclusive, and positive setting where students experienced what it feels like to be an active participant in the world of research. Within this context, critical mechanisms that gave rise to gains in research skills, confidence and self-efficacy for research, and STEM career aspirations included: (1) experiential education through interdisciplinary research experiences, (2) student independence and ownership balanced with expert researcher guidance and support, (3) formal and informal mentoring networks where students were mentored and where they mentored others, and (4) the establishment of an intentional learning community that advanced leadership, research skill building, perseverance, and reflection. Results from this research cannot be generalized beyond the context of the Lake Champlain REU, however, findings are in alignment with the body of literature that highlights the positive effects of REUs on STEM majors’ research skills, confidence and self-efficacy for research, and STEM career aspirations. Using mixed methods to identify and understand the within-program mechanisms that support student gains is a valuable new research approach for this field. Recognizing programmatic mechanisms across REU programs can lead to expansion, replication, and application of these models beyond one institution, resulting in more positive gains for more undergraduate STEM researchers.
HIV-associated anaemia before and after initiation of antiretroviral therapy at Art Centre of Minilik II Hospital, Addis Ababa, Ethiopia.

BACKGROUND HIV associated anaemia is always overseen and it could be a challenge for prognosis of patients who are taking ART. The prevalence of anemia due to HIV at the early stage of infection is more prevalent than in the late stage. Knowing the impact of HIV on the haematopotosis of HIV infected patients is very essential for the management and care of people living with HIV/AIDS. HIV related anemia decreases the quality of life and survival rate of HIV patients. OBJECTIVE The aim of this study is to determine prevalence of HIV associated anaemia before and after initiation of antiretroviral therapy (ART) in HIV infected adults. METHOD A retrospective record review was conducted on HIV infected patients before ART and had follow up at the ART Clinic of Minilik II Hospital Addis Ababa, Ethiopia. Haemoglobin measurement and CD4+ T cell count was measured using standard methodology at baseline and after 4 months of antiretroviral therapy (ART). Paired t -test was used to assess mean differences for haemoglobin and CD4+ T cell count before and after ART initiation. RESULTS Of the 230 study subjects 121 (52.6%) were anemic before ART. However, the prevalence of anemia after ART (37.4%) was significantly decreased (p < 0.05). The prevalence of anemia was higher in females than in males at base line (70.25% vs. 29.75%) (P = 0.017), and after ART treatment (69.23% vs. 30.77%) (P = 0.000). Mean CD4 cell count of study subjects was 112 cells/microl +/- 67/microl at baseline. The mean CD4+ T cell count is significantly increased after ART and found to be 211 cells/microl +/- 120/microl (p < 0.05). Significance association was observed between Hb aind CD4+ T cell count after ART (P = 0.011). CONCLUSIONS There was a decline in the prevalence of anemia and increment of mean CD4+ T cell count among HIV infected patients after ART. However, a number of HIV/AIDS patients had still anemia and their CD4+ T cell count is not improved. Thus, there should be a large scale and longitudinal study for further characterization of HIV related anemia.
Interaction of the Hepatitis B Core Antigen and the Innate Immune System1

Previous studies demonstrated that the primary APCs for the hepatitis B core Ag (HBcAg) were B cells and not dendritic cells (DC). We now report that splenic B1a and B1b cells more efficiently present soluble HBcAg to naive CD4+ T cells than splenic B2 cells. This was demonstrated by direct HBcAg-biotin-binding studies and by HBcAg-specific T cell activation in vitro in cultures of naive HBcAg-specific T cells and resting B cell subpopulations. The inability of DC to function as APCs for exogenous HBcAg relates to lack of uptake of HBcAg, not to processing or presentation, because HBcAg/anti-HBc immune complexes can be efficiently presented by DC. Furthermore, HBcAg-specific CD4+ and CD8+ T cell priming with DNA encoding HBcAg does not require B cell APCs. TLR activation, another innate immune response, was also examined. Full-length (HBcAg183), truncated (HBcAg149), and the nonparticulate HBeAg were screened for TLR stimulation via NF-κB activation in HEK293 cells expressing human TLRs. None of the HBc/HBeAgs activated human TLRs. Therefore, the HBc/HBeAg proteins are not ligands for human TLRs. However, the ssRNA contained within HBcAg183 does function as a TLR-7 ligand, as demonstrated at the T and B cell levels in TLR-7 knockout mice. Bacterial, yeast, and mammalian ssRNA encapsidated within HBcAg183 all function as TLR-7 ligands. These studies indicate that innate immune mechanisms bridge to and enhance the adaptive immune response to HBcAg and have important implications for the use of hepadnavirus core proteins as vaccine carrier platforms.
Non‐motor symptoms and quality of life in tremor dominant vs postural instability gait disorder Parkinson′s disease patients

To explore the differences in the features and impact on quality of life (QOL) of non‐motor symptoms (NMS) of tremor dominant (TD) and postural instability gait disorder (PIGD) phenotypes early Parkinson's disease (PD), as well as the determinants of poor QOL for TD and PIGD phenotypes.
Role of Lipid Peroxidation in Metalloporphyrin-Mediated Phototoxic Reactions in Neonatal Rats

ABSTRACT: Our group previously demonstrated dose-dependent mortality in neonatal rats treated with tin protoporphyrin and light. We hypothesize that lipid peroxidation may be responsible for the toxic effects of photosensitizing metalloporphyrins. Neonatal rat blood samples with or without metalloporphyrins (40 mM) were exposed to cool white light (20 μW/cm2/nm) for 30 min at 37°C. In the in vivo model, neonatal rat pups were given injections of 40 μmol of either tin protoporphyrin (4 mM), zinc protoporphyrin/kg body weight, or saline and placed over cool white light. The control animals were similarly treated but kept in the dark. After 3 h, the animals were killed, and their tissues were analyzed for malondialdehyde, conjugated dienes, and disappearance of polyunsaturated fatty acids as indices of lipid peroxidation. In all cases, the known photosensitizer tin protoporphyrin was associated with increased conjugated dienes in the liver and disappearance of polyunsaturated fatty acids and increased malondialdehyde in the liver and brain when animals were exposed to light. Zinc protoporphyrin was not associated with increased lipid peroxidation in the light except in the case of blood in vitro where malondialdehyde levels increased. We conclude that lipid peroxidation plays a role in metalloporphyrin-mediated phototoxicity in neonatal rat tissues.
The Mycobacterial 38-Kilodalton Glycolipoprotein Antigen Activates the Mitogen-Activated Protein Kinase Pathway and Release of Proinflammatory Cytokines through Toll-Like Receptors 2 and 4 in Human Monocytes

ABSTRACT Although the 38-kDa glycolipoprotein of Mycobacterium tuberculosis H37Rv is known to evoke prominent cellular and humoral immune responses in human tuberculosis (TB), little information is known about intracellular regulatory mechanisms involved in 38-kDa antigen (Ag)-induced host responses. In this study, we found that purified 38-kDa glycolipoprotein activates mitogen-activated protein kinases (MAPKs; extracellular signal-regulated kinase 1/2 [ERK1/2] and p38) and induces tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in human monocytes. When the 38-kDa Ag was applied to monocytes from TB patients and healthy controls, the activation of ERK1/2 and p38 MAPK and the subsequent cytokine secretion were greater in the monocytes from the active pulmonary TB patients than in monocytes from the healthy controls. Additionally, neutralizing antibodies for Toll-like receptor 2 (TLR2) or TLR4 significantly reduced the ERK1/2 and p38 activation induced by the 38-kDa protein when the antibodies were applied to HEK293 cells overexpressing TLR2 or TLR4 as well as human primary monocytes. Furthermore, the inhibition of TLR2 significantly, and that of TLR4 partially, decreased the 38-kDa Ag-induced secretion of TNF-α and IL-6 in human monocytes. The intact protein moieties of the 38-kDa protein were responsible for biologic activities by this Ag. These data collectively demonstrate that the 38-kDa glycolipoprotein, acting through both TLR2 and TLR4, induces the activation of the ERK1/2 and p38 MAPK pathways, which in turn play an essential role in TNF-α and IL-6 expression during mycobacterial infection.
Initiation of antiretroviral therapy at high CD4+ cell counts is associated with positive treatment outcomes

Objective:There is limited research investigating the possible mechanisms of how starting combination antiretroviral therapy (cART) at a higher CD4+ cell count decreases mortality. This study investigated the association between initiating cART with short-term and long-term achievement of viral suppression; emergence of any drug resistance and of an AIDS-defining illness (ADI); long-term treatment adherence; and all-cause mortality. Methods:This retrospective cohort study included 4120 naive patients who initiated cART between 2000 and 2012. Patients were followed until 2013, death or until the last contact date (varied by outcome). The main exposure was the interaction between period of cART initiation (2000–2006 and 2007–2012) and CD4+ cell count at cART initiation (<500 versus ≥500 cells/&mgr;l). We considered both baseline and longitudinal covariates. We fitted different multivariable models using cross-sectional and longitudinal statistical methods, depending on the outcome. Results:Patients who initiated cART with a CD4+ cell count at least 500 cells/&mgr;l in 2007–2012 had an increased likelihood of achieving viral suppression at 9 months and of maintaining an adherence level of at least 95% over time, and the lowest probability of developing any resistance and an ADI during follow-up. These patients were not the ones with the highest likelihood of maintaining viral suppression over time, most likely due to viral load blips experienced during the follow-up. Conclusion:The outcomes in this study likely play an important role in explaining the positive impact of early cART initiation on mortality. These results should alleviate some of the concerns clinicians may have when initiating cART in patients with high CD4+s as recommended by current treatment guidelines.
In Vivo Expression of an Alternatively Spliced Human Tumor Message That Encodes a Truncated Form of Cathepsin B

Cathepsin B is a lysosomal cysteine protease whose increased expression is believed to be linked to the malignant progression of tumors. Alternative splicing and the use of alternative transcription initiation sites in humans produce cathepsin B mRNAs that differ in their 5′- and 3′-untranslated ends. Some human tumors also contain cathepsin B-related transcripts that lack exon 3 which encodes the N-terminal signal peptide and 34 of the 62-amino acid inhibitory propeptide. In this study we show that one such transcript, CB(−2,3), which is missing exons 2 and 3, is likely to be a functional message in tumors. Thus, CB(−2,3) was found to be otherwise complete, containing the remainder of the cathepsin B coding sequence and the part of the 3′-untranslated region that is common to all previously characterized cathepsin B mRNAs in humans. Its in vitrotranslation product can be folded to produce enzymatic activity against the cathepsin B-specific substrate,N α-benzyloxycarbonyl-l-Arg-l-Arg-4-methylcoumaryl-7-amide. Endogenous CB(−2,3) from the metastatic human melanoma cell line, A375M, co-sediments with polysomes, indicating that it engages the eukaryotic translation machinery in these cells. Epitope-tagged forms of the truncated cathepsin B from CB(−2,3) are produced in amounts comparable to the normal protein after transient transfection into COS cells. Immunofluorescence microscopy and subcellular fractionation show this novel tumor form of cathepsin B to be associated with nuclei and other membranous organelles, where it is likely to be bound to the cytoplasmic face of the membranes. This subcellular distribution was different from the lysosomal pattern shown by the epitope-tagged, full-length cathepsin B in COS cells. These results indicate that the message missing exons 2 and 3 is likely to be translated into a catalytically active enzyme, and that alternative splicing (exon skipping) could contribute to the aberrant intracellular trafficking of cathepsin B that is observed in some human cancers.
Tumor vascularity, proliferation, and apoptosis in human melanoma micrometastases and macrometastases.

BACKGROUND Clinically undetectable or dormant metastases (micrometastases) probably account for disease recurrence, ie, clinically evident metastases, in patients after disease-free intervals of variable length. Recently developed animal models have shown that dormancy may potentially be explained by the fact that these micrometastases are not vascularized and have comparable rates of cellular proliferation and programmed cell death (apoptosis), enabling them to remain viable indefinitely but not to show progressive growth. OBSERVATION We report for the first time that melanoma micrometastases from humans are similarly not vascularized (mean number of microvessels, 10.2), have significantly lower rates of tumor cell proliferation (mean, 2.4%), comparable rates of proliferation and apoptosis (means, 2.4.% and 0.2%, respectively), compared with melanoma macrometastases, which have significantly greater tumor vascularity (mean number of microvessels, 18.7), higher rates of proliferation (mean, 18%), and higher rates of proliferation relative to apoptosis (means, 18% vs 1.6%). Tumor vascularity was quantified using the lectin Ulex europaeus agglutinin I to identify the number of microvessels per unit area (microscope ocular grid with an area of 7.84 x 10(-2) mm2 at x400 magnification). Melanoma cell proliferation rate was assessed with the MIB-1 antibody (Ki-67) as the number of positive nuclei per total number of tumor nuclei counted at x400 magnification. Apoptosis was quantified using the method of terminal deoxynucleotidyl transferase-medicated deoxyuridine triphosphate-biotin nick end labeling. The number of positive nuclei were quantified per total number of tumor nuclei; usually 200 tumor nuclei were counted at x400 magnification. CONCLUSION We report, for the first time, that human micrometastases demonstrate attributes, ie, the lack of significant tumor vascularity and low but comparable rates of proliferation and apoptosis, that may explain the dormant state.
Favourable outcome after peripartum cardiomyopathy: a ten-year study on peripartum cardiomyopathy in a university hospital.

INTRODUCTION Peripartum cardiomyopathy (PPCM) is an uncommon form of congestive heart failure, affecting obstetric patients around the time of delivery. The epidemiology of PPCM is infrequently reported. This study was undertaken to define the prevalence, presentation and outcome of PPCM among women giving birth in a teaching hospital in Malaysia. METHODS A retrospective case record analysis was conducted on all patients admitted and diagnosed with PPCM at the University Malaya Medical Centre, Kuala Lumpur, Malaysia, from 1 January 2000 to 31 December 2009. All deliveries were undertaken in the same hospital. RESULTS A total of 12 patients were diagnosed with PPCM during the ten-year study period. The prevalence of PPCM was 2.48 in 100,000 (1 in 40,322) live births. Nine women were diagnosed with PPCM within five months of delivery. Three women had twin pregnancies. There was one death in the group (mortality rate 8.3%). The mean left ventricular ejection fraction at the time of diagnosis was 28.9% ± 8.5% (range 15%-40%). Following the index event, left ventricular function normalised in six of the nine patients (66.7%) who underwent subsequent echocardiography one year later. All patients were treated with standard heart failure therapy. Two patients with normalised left ventricular function had subsequent pregnancies - one pregnancy was terminated at seven weeks and the other patient delivered uneventfully at full term. CONCLUSION PPCM is uncommon. The outcome in our series was favourable, with 66.7% of patients with PPCM recovering their left ventricular function. The mortality rate was 8.3%.
OEPARTEMENT TOEGEPASTE ECONOMISCHE WETENSCHAPPEN

Abstract International organisations are increasingly urging governments to support the dissemination of new organisational concepts. Research into the efforts made by the business community in its quest for new organisational concepts and permanent monitoring of the effects of organisational transformation are necessary tools for supporting these policy lines. Since 1980 a variety of organisation surveys have been undertaken in an effort to evaluate the extent and effects of workplace innovation. Comparisons of the findings or results of these major organisation surveys are fraught with major difficulties because the choices of methodology and survey design differ widely. Moreover, little information is currently available about the methodological limitations of these organisation surveys. This paper therefore concentrates on the methodological design of 16 major organisation surveys. The objectives of the paper can be described as follows: