Miracle-dz/UniFAR-FTUs huggingface dataset:
Children With Morbid Obesity Benefit Equally as Children With Overweight and Obesity From an Ongoing Care Program.

CONTEXT Despite stabilization of childhood overweight and obesity prevalence, there is a shift toward more severe degrees of obesity, which results in an increasing prevalence of children with morbid obesity. Prior studies demonstrated that lifestyle modification without ongoing treatment has only a modest and not sustainable effect in children with morbid obesity. This suggests that a chronic care model is necessary for long-term effects on weight management and health. OBJECTIVE This study aimed to evaluate the effect of an ongoing lifestyle intervention in children with morbid obesity in comparison with children with overweight and obesity. DESIGN AND SETTING This was a nonrandomized prospective intervention study with 12- and 24-month followup at the Centre for Overweight Adolescent and Children's Healthcare. PATIENTS AND INTERVENTION Children and adolescents (n = 100 females and 72 males) with overweight, obesity, or morbid obesity were given long-term, outpatient, tailored lifestyle intervention. MAIN OUTCOME MEASURE Body mass index (BMI) z score was measured. RESULTS In children with morbid obesity, 12- and 24-month interventions resulted in a decrease of BMI z score of -0.13 ± 0.25 (P = .001) and -0.23 ± 0.32 (P = .01) respectively, whereas weight status category improved to obese in 21% and 25% of the children. Cardiovascular risk parameters including serum total cholesterol, low-density lipoprotein cholesterol, glycosylated hemoglobin (HbA1c), and diastolic blood pressure significantly improved after 1-year intervention in the complete group. Most important, BMI z score as well as cardiovascular risk parameters improved to a similar degree in children with overweight, obesity, and morbid obesity. CONCLUSIONS Children with overweight, obesity, and morbid obesity benefit equally from an ongoing, outpatient, tailored lifestyle intervention, and demonstrate significant weight loss and improvement of cardiovascular risk parameters.
A putative role for TIP and PIP aquaporins in dynamics of leaf hydraulic and stomatal conductances in grapevine under water stress and re-watering.

We examined the role of aquaporins (AQPs) in regulating leaf hydraulic conductance (Kleaf ) in Vitis vinifera L. (cv Chardonnay) by studying effects of AQP inhibitors, and AQP gene expression during water stress (WS) and recovery (REC). Kleaf was measured after 3 h of petiole perfusion with different solutions and to introduce inhibitors. The addition of 0.1 mm HgCl2 to 15 mm KCl reduced Kleaf compared with perfusion in 15 mM KNO3 or KCl, and these solutions were used for leaves from control, WS and REC plants. Perfusion for 3 h did not significantly alter stomatal conductance (gs ) though expression of VvTIP1;1 was increased. WS decreased Kleaf by about 30% and was correlated with gs . The expression of VvTIP2;1 and VvPIP2;1 correlated with Kleaf , and VvTIP2;1 was highly correlated with gs . There was no association between the expression of particular AQPs during WS and REC and inhibition of Kleaf by HgCl2 ; however, HgCl2 treatment itself increased expression of VvPIP2;3 and decreased expression of VvPIP2;1. Inhibition by HgCl2 of Kleaf only at early stages of WS and then after REC suggested that apoplasmic pathways become more important during WS. This was confirmed using fluorescent dyes confined to apoplasm or preferentially accumulated in symplasm.
Prospective observational cohort studies for studying rare diseases: the European PedNet Haemophilia Registry

Haemophilia is a rare disease. To improve knowledge, prospective studies of large numbers of subjects are needed. To establish a large well‐documented birth cohort of patients with haemophilia enabling studies on early presentation, side effects and outcome of treatment. Twenty‐one haemophilia treatment centres have been collecting data on all children with haemophilia with FVIII/IX levels up to 25% born from 2000 onwards. Another eight centres collected data on severe haemophilia A only. At baseline, details on delivery and diagnosis, gene mutation, family history of haemophilia and inhibitors are collected. For the first 75 exposure days, date, reason, dose and product are recorded for each infusion. Clinically relevant inhibitors are defined as follows: at least two positive inhibitor titres and a FVIII/IX recovery <66% of expected. For inhibitor patients, results of all inhibitor‐ and recovery tests are collected. For continued treatment, data on bleeding, surgery, prophylaxis and clotting factor consumption are collected annually. Data are downloaded for analysis annually. In May 2013, a total of 1094 patients were included: 701 with severe, 146 with moderate and 247 with mild haemophilia. Gene defect data were available for 87.6% of patients with severe haemophilia A. The first analysis, performed in May 2011, lead to two landmark publications. The outcome of this large collaborative research confirms its value for the improvement of haemophilia care. High‐quality prospective observational cohorts form an ideal source to study natural history and treatment in rare diseases such as haemophilia.
Occurrence of oval‐type cells in hepatitis B virus—associated human hepatocarcinogenesis

Proliferation of a new population of epithelial cells with distinct structure, as well as cytokeratin and α‐fetoprotein expression, was observed in nonneoplastic liver tissues from 14 cases (13 hepatitis B virus—positive) of human hepatocellular carcinoma. These cells were characterized by oval nuclei; scant, pale cytoplasm; small cell size; and cross‐reaction with a monoclonal antibody against rat oval cells. These putative human oval cells were strongly positive for cytokeratin 19 and displayed considerable heterogeneity in α‐fetoprotein and albumin expression. The oval cells were most prominent in actively regenerating nodules and in liver tissue surrounding the cancer. Oval cells and transitional types of cells appear to be the principal producers of α‐fetoprotein in the regenerating liver. Cancer cells positive for cytokeratins 8, 18 and 19 were observed in half the hepatocellular carcinomas studied. The data suggest that a new cell population structurally similar to oval cells seen in early stages of chemical hepatocarcinogenesis in rats is consistently present in regenerating liver lesions associated with human hepatocellular carcinoma. Furthermore, it is possible that the proliferation of these oval‐type cells may partly account for the elevation of serum α‐fetoprotein frequently seen in precancerous stages of hepatitis B virus—associated human hepatocellular carcinoma.
Caudal clonidine and bupivacaine for combined epidural and general anaesthesia in children

Background: Clonidine produces analgesia by actions on α2‐ad‐renoceptors and enhances both sensory and motor blockade from epidural injection of local anaesthetics. Low‐dose clonidine has been used so far for caudal injection in children. Our aim was to study the perioperative effects of high‐dose caudal clonidine when added to low concentration of bupivacaine for combined epidural and general anaesthesia in children.
The developmental paradigm, reading history sideways, and family change

The developmental paradigm, reading history sideways, and cross-cultural data have converged to exert a profound influence on social scientists and ordinary people. Through the use of these tools, social scientists of the 1700s and 1800s concluded that family patterns in northwest Europe had undergone many substantial changes before the early 1800s. These conclusions were accepted until the last several decades of the 1900s, when almost all were seriously challenged; many were declared to be myths. Further, the developmental paradigm, reading history sideways, and the conclusions of generations of social scientists created a package of ideas—developmental idealism—that subsequently became a powerful influence for family change in many parts of the world during the past two centuries. This developmental idealism has been a substantial force for changing living arrangements, marriage, divorce, gender relations, intergenerational relationships, and fertility.
Increased expression of oncogene-induced senescence markers during cervical squamous cell cancer development.

PURPOSE To investigate the expression of p15(INK4b), p16(INK4a) and p21(Waf1/Cip1) in specimens from cases of normal cervical epithelium (NCE), cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC), and to evaluate whether there is evidence implicating oncogene-induced senescence (OIS) in cervical squamous cell cancer development. METHODS The immunohistochemical expression of p15(INK4b), p16(INK4a) and p21(Waf1/Cip1) were investigated in formalin-fixed paraffin-embedded specimens from 19 NCE, 51 CIN and 21 SCC cases, respectively. Comparisons among different groups for each marker were performed with Chi-square test. RESULTS The expression of p15(INK4b), p16(INK4a) and p21(Waf1/Cip1) were significantly higher in both CIN and SCC compared to NCE. Furthermore, the expression of p15(INK4b) and p21(Waf1/Cip1) was significantly higher in CIN П compared to CIN І, and these expressions were statistically higher in CIN Ш compared to CIN П, respectively. The p16(INK4a) expression was significantly higher in CIN Ш compared to CIN І. CONCLUSIONS The results suggested that the senescence programs mediated by p15(INK4b), p16(INK4a) and p21(Waf1/Cip1) were activated during the stage of CIN and SCC, and demonstrated that senescence may play important role in preventing from NCE to SCC.
A preliminary study of physiological characteristics of temperature-sensitive mutants of influenza virus.

Summary A collection of temperature-sensitive mutants of influenza virus was examined in an attempt to define their defective functions when grown at the restrictive temperature and to detect alterations in the physical properties of virus grown at the permissive temperature. Three mutants were found to be defective in the synthesis of haemagglutinin. The haemagglutinin of the fourth mutant was shown to be functionally and antigenically normal but it could not be detected at the cell surface. A similar absence of function at the cell surface was demonstrated for the neuraminidase of two other mutants. Two genetically identical haemagglutinin-defective mutants were also partially defective in the incorporation of uridine into virus RNA but this pleiotropy did not extend to the neuraminidase. Although immunofluorescence showed that all mutants were able to synthesize ribonucleoprotein, the ribonucleoprotein of one mutant was unable to migrate from the nucleus. The heat-sensitivity of one mutant could not be correlated with defective function. The physical properties of mutant virus particles did not differ from the parent strain except that the infectivity of one mutant was more labile and another mutant had an altered affinity for erythrocytes of different species. These defects did not correlate with known defects in multiplication.
The Divergently Transcribed Streptococcus parasanguis Virulence-Associated fimA Operon Encoding an Mn2+-Responsive Metal Transporter and pepO Encoding a Zinc Metallopeptidase Are Not Coordinately Regulated

ABSTRACT The study of how bacteria respond to and obtain divalent metal ions provides insight into the regulation of virulence factors in the host environment. Regulation of metal permease operons in gram-positive bacteria may involve the binding of metal-responsive repressors to palindromic domains in their control regions. The Streptococcus parasanguis fimA operon, which encodes an ATP-binding cassette (ABC) transporter system with sequence homology to the LraI family of metal transporters, possesses a palindromic regulatory region with high homology to that of the Streptococcus gordonii ScaR binding domain. Mapping of the promoter and regulatory regions of fimA and the divergently transcribed pepO gene, which encodes a zinc metalloendopeptidase, indicated that their promoter and regulatory elements overlap. fimA had one transcriptional start site, whereas pepO had three. Analysis of truncated versions of the pepO promoter suggested that all three transcriptional start sites are functional. Analysis of promoter activity under various environmental conditions indicated that the fimA operon promoter and the pepO promoter are not coordinately regulated. The fimA operon is responsive to changes in Mn2+ concentration, but the pepO promoter is not. A S. parasanguis fimA mutant showed a growth deficiency under conditions of limiting Mn2+. This deficiency was not alleviated by compensation with either Mg2+ or Fe3+. Wild-type S. parasanguis could take up Mn2+ and Fe3+, while the fimA mutant showed a marked reduction in this ability. These data suggested that FimA is a component of a metal transporter system capable of transporting both Mn2+ and Fe3+. FimA expression itself was shown to be responsive to Mn2+ concentration, but not to availability of Fe3+ or Mg2+.
Sparfloxacin Selects Gyrase Mutations in First-Step Mycoplasma hominis Mutants, whereas Ofloxacin Selects Topoisonmerase IV Mutations

ABSTRACT The role of mutations in the genes for GyrA and ParC in quinolone resistance in Mycoplasma hominis was studied. Selection with sparfloxacin gave mutations at GyrA83 (Ser→Leu;Escherichia coli numbering) or GyrA87 (Glu→Lys), and mutants had increased levels of resistance to sparfloxacin (8- to 16-fold) but not to ofloxacin. Selection with ofloxacin gave changes at ParC80 (Ser→Ile) or ParC84 (Glu→Lys), and mutants were four- to eightfold more resistant to ofloxacin but not to sparfloxacin. Selection of second-step mutants from strains with ParC mutations with either quinolone yielded double mutants with additional mutations at GyrA83 (Ser→Trp or Ser→Leu) or GyrA87 (Glu→Lys). Second-step selection of GyrA mutants gave additional mutations at ParC80 (Ser→Ile) or ParC84 (Glu→Lys). Two-step mutants showed high levels of resistance to ofloxacin (MICs, 64 to 128 μg/ml) and moderate levels of resistance to sparfloxacin (MICs, 2 to 8 μg/ml). The primary target of ofloxacin in first-step mutants of Mycoplasma hominis was ParC, whereas that for sparfloxacin was GyrA.
Sex Differences in Mechanical Properties of the Achilles Tendon: Longitudinal Response to Repetitive Loading Exercise

Abstract Lepley, AS, Joseph, MF, Daigle, NR, Digiacomo, JE, Galer, J, Rock, E, Rosier, SB, and Sureja, PB. Sex differences in mechanical properties of the Achilles tendon: Longitudinal response to repetitive loading exercise. J Strength Cond Res 32(11): 3070–3079, 2018—Sex differences have been observed in the mechanical properties of the Achilles tendon, which may help to explain the increased risk of injury in men. However, the response and recovery of tendon mechanics to repetitive loading exercise, as well as sex-dependent responses, are not well understood. The purpose of our study was to compare Achilles tendon mechanical properties between men and women before, immediately after, and 60 minutes after a repetitive loading exercise. Seventeen female (age: 24.0 ± 3.9 years; height: 167.4 ± 6.9 cm; and mass: 64.9 ± 8.5 kg) and 18 male (age: 23.9 ± 2.4 years; height: 179.2 ± 5.09 cm; and mass: 78.4 ± 8.7 kg) recreationally active individuals volunteered. Using isokinetic dynamometry and diagnostic ultrasound, baseline levels of Achilles tendon force, elongation, stiffness, stress, strain, and Young's modulus were assessed before 100 successive calf-raise exercises using a Smith machine at 20% of participant body mass. Outcomes were reassessed immediately and 60 minutes after exercise. Women exhibited less Achilles tendon force, stiffness, stress, and modulus compared with men, regardless of time point. Both sexes responded to repetitive loading exercise similarly, with immediate decreases in mechanical properties of the Achilles tendon from baseline to immediately after exercise. Tendon properties were observed to be equal to baseline values at 60-minute postexercise. Baseline differences in tendon properties may help to explain the disparity in injury risk because both sexes responded to and recovered from exercise similarly. Future research should aim to include additional time points (both leading up to and after 60 minutes), and assess tendon responses to more sport-specific activities, while also including patients diagnosed with Achilles tendon injuries.
Group interpersonal psychotherapy for depression in rural Uganda: 6-month outcomes

Background A randomised controlled trial comparing group interpersonal psychotherapy with treatment as usual among rural Ugandans meeting symptom and functional impairment criteria for DSM–IV major depressive disorder or sub-threshold disorder showed evidence of effectiveness immediately following the intervention. Aims To assess the long-term effectiveness of this therapy over a subsequent 6-month period. Method A follow-up study of trial participants was conducted in which the primary outcomes were depression diagnosis, depressive symptoms and functional impairment. Results At 6 months, participants receiving the group interpersonal psychotherapy had mean depression symptom and functional impairment scores respectively 14.0 points (95% CI 12.2–15.8; P < 0.0001) and 5.0 points (95% CI 3.6–6.4; P < 0.0001) lower than the control group. Similarly, the rate of major depression among those in the treatment arm (11.7%) was significantly lower than that in the control arm (54.9%) (P < 0.0001). Conclusions Participation in a 16-week group interpersonal psychotherapy intervention continued to confer a substantial mental health benefit 6 months after conclusion of the formal intervention.
CYP1A2 genotype modifies the association between coffee intake and the risk of hypertension

Objectives The longitudinal relationship between coffee use and hypertension is still controversial. Cytochrome P450 1A2 (CYP1A2) is the main responsible enzyme for the metabolism of caffeine. The aim of the present study was to investigate the effect of coffee intake on the risk of developing hypertension needing antihypertensive treatment in individuals stratified by CYP1A2 genotype. Design We assessed prospectively 553 young White individuals screened for stage 1 hypertension. Coffee intake was ascertained from regularly administered questionnaires. Incident physician-diagnosed hypertension was the outcome measure. Genotyping of CYP1A2 SNP was performed by real time PCR. Results During a median follow-up of 8.2 years, 323 individuals developed hypertension. For carriers of the slow *1F allele (59%), hazard ratios of hypertension from multivariable Cox analysis were 1.00 in abstainers (reference), 1.72 (95%CI, 1.21–2.44) in moderate coffee drinkers (P = 0.03), and 3.00 (1.53–5.90) in heavy drinkers (P = 0.001). In contrast, hazard ratios for coffee drinkers with the rapid *1A/*1A genotype were 0.80 (0.52–1.23, P = 0.29) for moderate drinkers and 0.36 (0.14–0.89, P = 0.026) for heavy drinkers. In a two-way ANCOVA, a gene × coffee interactive effect was found on follow-up changes in systolic (P = 0.000) and diastolic (P = 0.007) blood pressure. Urinary epinephrine was higher in coffee drinkers than abstainers but only among individuals with slow *1F allele (P = 0.001). Conclusion These data show that the risk of hypertension associated with coffee intake varies according to CYP1A2 genotype. Carriers of slow *1F allele are at increased risk and should thus abstain from coffee, whereas individuals with *1A/*1A genotype can safely drink coffee.
Spatio-Temporal Ecology and Management of Cabbage Maggot

Abstract This study analyzes the spatio-temporal dispersion patterns of the cabbage maggot (Delia radicum L.) (Diptera: Anthomyiidae) infestation in rutabagas and turnips in Oregon and suggests ways to exploit the spatial and temporal ecology of the cabbage maggot to improve management of the pest. The patchy distribution of cabbage maggots arises from a combination of first-order effects driven by spatial heterogeneity and second-order effects driven by spatial autocorrelation. The intensity of cabbage maggot infestations varied from year to year. Within a given year, damage rates tended to be higher in rutabagas than turnips, in crops planted earlier in the season, and in fields near nurseries and houses. Nonsignificant first-order effects included soil texture, distance from river, proximity to maggot sources (other than cultivated fields), type of vegetation on field borders, field manager, field area, and perimeter. Second-order effects were processes intrinsic to the population and would give rise to patchiness even in a homogeneous environment. For example, adults may be attracted to others of their species or eggs may be deposited in batches. The locations of patches arising from second-order effects cannot be predicted from knowledge of environmental covariates. However, cabbage maggot does not tend to disperse far, and existing patches tend to give rise to other patches nearby at a later time. We found elevated damage rates in spring fields planted near fields that were heavily damaged the previous fall and in fields planted late in the season near fields that had heavy damage early in the season.
Counting logics and games with counters

In verification and synthesis, properties or models of interest are often quantitative, and many quantitative aspects involve counting. For example, one might be interested in the amount of memory required by a system, how many simultaneous requests a system has to process along a run, or how many infixes belonging to some regular language a word contains. In this thesis, we study two models of infinite games with counters as well as two quantitative counting logics. The first game model we consider is that of counter parity games. In these quantitative games, a finite set of counters is updated along the edges. Payoffs of finite plays are obtained via the counters, while payoffs of infinite plays are determined via a parity condition. The games are played by a maximizing and a minimizing player, and satisfying the parity condition is good for the maximizing player. We prove that the value of a counter parity game can be computed, and give an algorithm to do so. The key step in the algorithm is to solve the unboundedness problem for the value. This is done with the help of a game with imperfect recall, for which we show that finite-memory strategies suffice for the player suffering from imperfect recall. In mean counter games, the second class of games studied in this thesis, the payoff of infinite plays is obtained via a mean-payoff condition on a special counter. We prove that the unboundedness problem for the value can be solved in games with only one counter. Furthermore, we show that the exact value can be computed in one-counter single-player games. The first logic we study is Qμ[#MSO], a counting logic based on the quantitative μ-calculus. This logic is designed specifically for transition systems where the states are labeled with finite relational structures. Counting is introduced to Qμ with the help of counting terms of monadic secondorder logic, which are evaluated on the relational structures the states are labeled with. We prove, via a reduction to counter parity games, that the model-checking problem for Qμ[#MSO] is decidable on a generalization of pushdown systems. We also consider a quantitative counting extension of monadic secondorder logic called qcMSO. In this logic, we adapt the classical interpretations of disjunctions as maxima and conjunctions as minima and use counting atoms ∣X ∣ to count the sizes of sets. We investigate the connections between qcMSO and two other extensions of MSO, namely costMSO and MSO+U. We prove that the qcWMSO-theory of the natural numbers with order is decidable via a reduction to the model-checking problem for an extension of FO+RR on resource-automatic structures. We also provide decomposition algorithms for qcMSO on the natural numbers with order and the infinite binary tree.