Miracle-dz/UniFAR-FTUs huggingface dataset:
A Policy Network Explanation of Biotechnology Policy Differences between the United States and Canada

Canada has a more restrictive biotechnology policy than the United States. Adopting a similar-cases-research-design, this article shows that policy networks explain this difference. The overlapping nature and the boundary between the multiple networks relevant to biotechnology in each country are distinct. In the United States, two policy networks deal with biotechnology. One primarily handles agricultural plants, while the other deals with food; key state actors overlap. In contrast, networks in Canada are separated between those dealing with regulation with two overlapping networks assessing environmental and health risks, and a network to manage biotechnology promotion. Promotion and regulation thus constitute a network boundary in Canada, but not in the United States, where networks deal with these two issues simultaneously. American networks have promoted beliefs favourable to more permissive regulatory preferences than the Canadian environmental and health risk assessment networks and American biotechnology policies are therefore even more permissive than those of Canada.
Transformation of Stomoxys calcitrans with a Hermes gene vector

The ability of the Hermes transposable element to function as a germ line transformation vector was tested in the stable fly, Stomoxys calcitrans. Plasmid‐based transposable element mobility assays indicated moderate mobility of Hermes in this species. Germline transformants were created using a Hermes element containing the enhanced green fluorescent protein (EGFP) under the regulatory control of the promoter from Actin5C gene of Drosophila melanogaster. Approximately 4% of the fifty‐five adults that developed from the 1903 G0 embryos injected with the vector produced transgenic progeny. In the four transgenic lines established, the EGFP expression pattern was distinctly nonuniform and levels of expression were low. Promoters other than the one from the Actin5C gene of D.melanogaster should be considered for widespread, constitutive expression. All transgenic lines contained multiple (2–4) integrated Hermes elements. Hermes integration events occurred through a canonical cut‐and‐paste mechanism.
The Spillover Effect of Antitrust Enforcement

We address the question of how the threat of antitrust enforcement spills over from one industrial submarket to another. We build a formal model describing the technology through which an initial antitrust indictment can reveal evidence of additional infractions in the same industry. Our approach is organized around the belief that the flow of information on antitrust violations is closely linked to commodity flows. As it turns out, our method proves able to explain the differential response of highway construction cartels in various states to recent changes in the severity of antitrust sanctions.
Prognostic value of histogram analysis in advanced non-small cell lung cancer: a radiomic study

Introduction Quantitative assessment of heterogeneity by histogram analysis (HA) of tumor images can potentially provide a non-invasive prognostic biomarker. We assessed the prognostic value of HA and evaluated a correlation with molecular signature. Results CT scans performed between July 2009 and January 2015 from 692 patients were reviewed. HA was performed on scans from 313 patients in the training dataset and 108 in the validation dataset. Median follow-up were 33.7 months [range: 1.7 - 65.5] and 29 months [range: 1.1 - 35.6] with a median overall survival (OS) of 11.7 months [95%CI: 10.7 - 13.1] and 9.5 months [95%CI: 7.9 - 12.7] respectively. Primary mass entropy in coarse texture with spatial filter 3.3 was prognostic for OS in a multivariate Cox analysis (HR: 1.3 [95%CI: 1.1 - 1.5], p=0.001). Results were not reproduced in our validation set and no correlation with molecular signature was identified. Materials and Methods HA using filtration-histogram method was applied to the region of interest on the primary tumor in enhanced-CT acquired as diagnostic/staging routine, from a cohort of patients with advanced non-small cell lung cancer (NSCLC) treated with platinum-based chemotherapy. The resultants parameters were prospectively applied to a validation dataset. CT scans, clinical and molecular data were retrospectively collected. Cox proportional hazard models were used for survival analysis and Wilcoxon test for correlations. Conclusion Primary mass entropy was significantly associated with survival in the training set but was not validated in the validation cohort, raising doubt over the reliability of published data from small cohorts.
What Every Investor Should Know About Commodities, Part Ii: Multivariate Return Analysis

In this paper we study the univariate return properties of a large variety of commodity futures. Our analysis shows that the volatility of commodity futures is comparable to that of US large cap stocks. Yet, with the exception of energy, a consistently positive risk premium is lacking in commodity futures. We also find that for many commodities, futures returns and volatility can vary considerably over different phases of the business cycle, under different monetary conditions as well as with the shape of the futures curve. Skewness in commodity futures returns is largely insignificant, whereas kurtosis is significantly positive and comparable to that of US large cap stocks. In almost all commodities we find significant degrees of autocorrelation, which affects the properties of longer horizon returns.
Understanding Residents' Attitudes toward the Development of Industrial Tourism in a Former Mining Community

The topic of residents' attitudes toward tourism development has been analyzed by many authors, but there have been few studies that have included: (1) the impacts resulting from tourism perceived by residents of a particular region in a very early stage of tourism development, where, consequently, this industry is not yet economically important; (2) the relationship between the impacts of tourism perceived by residents and their degree of satisfaction with their community; and (3) a specific tourism segment, such as industrial tourism. The article presented here addresses these gaps in the literature, and focuses attention on the attitudes of the residents of Minas de Riotinto—a locality of the Spanish province of Huelva, traditionally involved in mining activity, which is now in decline and in the very initial phase of tourism development—toward greater development of tourism in their municipality, and on the factors that may determine and explain those attitudes.
Origin of year-long bean (Phaseolus dumosus Macfady, Fabaceae) from reticulated hybridization events between multiple Phaseolus species.

BACKGROUND AND AIMS Improved understanding of the secondary gene pools of crops is essential for advancing genetic gain in breeding programmes. Common bean, Phaseolus vulgaris, is a staple crop with several wild relatives in its secondary gene pool. The year-long bean, P. dumosus, an important crop in Guatemala, is considered particularly closely related to P. vulgaris and a potential source of novel variation. However, the genetic diversity and relationship to other Phaseolus species of P. dumosus remain unclear. METHODS We conducted the first comprehensive investigation of P. dumosus genetic diversity using both nuclear and chloroplast genome markers. Our nuclear marker set included over 700 markers present within the Phaseolus DArT (Diversity Arrays Technology) array, which we applied to P. dumosus and other relatives of P. vulgaris (including every secondary gene pool species: P. acutifolius, P. albescens, P. coccineus and P. costaricensis). KEY RESULTS Phaseolus dumosus arose from hybridization of P. vulgaris and P. coccineus, followed by at least two later hybridizations with sympatric congener populations. Existing P. dumosus collections have low genetic diversity. CONCLUSIONS The under-utilized crop P. dumosus has a complex hybrid origin. Further sampling in the region in which it arose may uncover additional germplasm for introgressing favourable traits into crops within the P. vulgaris gene pool.
Optimal Patent Design and the Diffusion of Innovations

Innovators who have made significant breakthroughs may be tempted to get a head start in developing the applications of a new discovery before commercializing any new product. We consider how this socially undesirable waiting period and the pattern of development of subsequent innovations are affected by two patent protection regimes which we call "length" and "scope" protection. Our main finding is that the scope of the patent, and not its length, is the dimension that should be used to induce early disclosure of fundamental innovations while still preserving firms' incentive to do R&D. Furthermore, the optimal protection increases with rivalry in the markets for applications.
Alternative Splicing in Class V Myosins Determines Association with Rab10*

Rab proteins influence vesicle trafficking pathways through the assembly of regulatory protein complexes. Previous investigations have documented that Rab11a and Rab8a can interact with the tail region of myosin Vb and regulate distinct trafficking pathways. We have now determined that a related Rab protein, Rab10, can interact with myosin Va, myosin Vb, and myosin Vc. Rab10 localized to a system of tubules and vesicles that have partially overlapping localization with Rab8a. Both Rab8a and Rab10 were mislocalized by the expression of dominant-negative myosin V tails. Interaction with Rab10 was dependent on the presence of the alternatively spliced exon D in myosin Va and myosin Vb and the homologous region in myosin Vc. Yeast two-hybrid assays and fluorescence resonance energy transfer studies confirmed that Rab10 binding to myosin V tails in vivo required the alternatively spliced exon D. In contrast to our previous work, we found that Rab11a can interact with both myosin Va and myosin Vb tails independent of their splice isoform. These results indicate that Rab GTPases regulate diverse endocytic trafficking pathways through recruitment of multiple myosin V isoforms.
Australian clinical practice guidelines for the diagnosis and management of Barrett's esophagus and early esophageal adenocarcinoma

Barrett's esophagus (BE), a common condition, is the only known precursor to esophageal adenocarcinoma (EAC). There is uncertainty about the best way to manage BE as most people with BE never develop EAC and most patients diagnosed with EAC have no preceding diagnosis of BE. Moreover, there have been recent advances in knowledge and practice about the management of BE and early EAC. To aid clinical decision making in this rapidly moving field, Cancer Council Australia convened an expert working party to identify pertinent clinical questions. The questions covered a wide range of topics including endoscopic and histological definitions of BE and early EAC; prevalence, incidence, natural history, and risk factors for BE; and methods for managing BE and early EAC. The latter considered modification of lifestyle factors; screening and surveillance strategies; and medical, endoscopic, and surgical interventions. To answer each question, the working party systematically reviewed the literature and developed a set of recommendations through consensus. Evidence underpinning each recommendation was rated according to quality and applicability.
[The effect of age in a planning and arranging task ('Mexican pyramid') among schoolchildren].

AIM To analyze the influence of age on the performance of a planning and organization task, two skills included in the executive functions. SUBJECTS AND METHODS Performance of the task 'Mexican pyramid' from the Evaluacion Neuropsicologica Infantil (ENI) were analyzed on 239 school age children from México and Colombia, distributed in six age groups. Five measures were analyzed: number of correct designs, number of movements per design and execution time in the correct designs, number of correct designs built with the minimum of movements and its execution time. RESULTS There was a significant effect of age on the five measures. Comparison between groups showed that younger children (5 to 6 years old) had a smaller number of correct designs, while children from 5 to 8 years old need more movements. Execution time showed a greater number of differences between groups. Analyzing the 11 items of the task, it was evident that difficulty level is related with the number of movements and the novelty of rules. CONCLUSIONS Our results suggest that accuracy of performance, the number of moves as well as the speed of performance are useful markers of the development of the executive functions. Our results support the notion that the development of planning and organization; is fast in the first years of the school age, and it slows down on adolescence.
Statins Modulate Oxidized Low-Density Lipoprotein-Mediated Adhesion Molecule Expression in Human Coronary Artery Endothelial Cells: Role of LOX-1

LOX-1, a receptor for oxidized low-density lipoprotein (ox-LDL), plays a critical role in endothelial dysfunction and atherosclerosis. LOX-1 activation also plays an important role in monocyte adhesion to endothelial cells. A number of studies show that 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) reduce total LDL cholesterol and exert a cardioprotective effect. We examined the modulation of LOX-1 expression and its function by two different statins, simvastatin and atorvastatin, in human coronary artery endothelial cells (HCAECs). We observed that ox-LDL (40 μg/ml) treatment up-regulated the expression of E- and P-selectins, VCAM-1 and ICAM-1 in HCAECs. Ox-LDL mediated these effects via LOX-1, since antisense to LOX-1 mRNA decreased LOX-1 expression and subsequent adhesion molecule expression. Pretreatment of HCAECs with simvastatin or atorvastatin (1 and 10 μM) reduced ox-LDL-induced expression of LOX-1 as well as adhesion molecules (all P < 0.05). A high concentration of statins (10 μM) was more potent than the low concentration (1 μM) (P < 0.05). Both statins reduced ox-LDL-mediated activation of the redox-sensitive nuclear factor-κB (NF-κB) but not AP-1. These observations indicate that LOX-1 activation plays an important role in ox-LDL-induced expression of adhesion molecules. Inhibition of expression of LOX-1 and adhesion molecules and activation of NF-κB may be another mechanism of beneficial effects of statins in vascular diseases.
Oxidative capacity of skeletal muscle fibres in racehorses: histochemical versus biochemical analysis.

The oxidative capacity of skeletal muscle fibre types was evaluated histochemically using the nicotinamide dinucleotide diaphorase (NADH-D) staining, and biochemically by measuring the activity of citrate synthase (CS) in both whole muscle samples and in pools of fibres of identified type. Duplicate determinations of the NADH-D staining pattern resulted in standard deviations (sd) between duplicates of 6 and 11 per cent for two observers. The NADH-D pattern was found to differ between observers. Duplicate determinations of CS activity in the same fibre pools resulted in an sd value of 2.9 mumol/g/min. Measurements of whole muscle CS activity did not provide information about the distribution of oxidative capacity among fibre types. The NADH-D stain and CS activity in fibre pools both showed that, in general, type I and IIA fibres had a higher oxidative capacity than type IIB fibres. Biochemical techniques also showed, however, that the CS activity in type I and IIA fibres of different horses could vary as much as twofold, whereas the NADH-D rating showed a high intensity staining for most type I and IIA fibres in all horses. Furthermore, type IIB fibres received a lower NADH-D rating than the other fibre types even when the CS activities were quite similar. For purposes of research, biochemical measurement of oxidative capacity in individual muscle fibre types provides valuable quantitative and comparative information. The ease of histochemical NADH-D staining in comparison to fibre dissections makes this technique more practical for routine estimates of the distribution of oxidative capacity among muscle fibres. (ABSTRACT TRUNCATED AT 250 WORDS)
Development of a Questionnaire to Assess Drug Abuse among High School Students of Isfahan Province, Iran: An Action Research

Background: Considering the problem of drug abuse in Iran especially in adolescents and the youth, recent alterations in drug abuse rate and its trend, the necessity to have local information about this problem, applied research has a determining role in management of this problem and making proper decisions. Therefore, the current study was conducted to develop a questionnaire to assess the status of drug abuse among high school students of Isfahan Province, Iran. Methods: This cross-sectional study was conducted out in 2009 in 20 cities of Isfahan Province. A researcher-made questionnaire was developed to determine knowledge, attitude, and practice of high school students regarding addictive drugs and their associated causes. This was accomplished by recruiting 7137 students who were selected by multistage random cluster sampling. Results: The designed questionnaire identified the status quo of drug abuse according to age, gender, and different cities of Isfahan Province. We also accessed information about the type of abused drug, the most common causes of drug abuse for the first time, the most important causes of drug abuse, mean age of abusers and mean age at the first abuse, common time and locations of drug abuse, and the most common routes of drug abuse according to gender as well as urban and rural areas of Isfahan Province. Reliability of the questionnaire, based on the calculated Cronbach's alpha coefficient, was 77% considering a cut-off point of 0.07. Conclusions: According to the obtained results, the designed questionnaire is capable to assess the drug abuse status among high school students of Isfahan Province. Regarding the importance of teenage years in forming the future behaviors of adolescents and the opportunities provided at schools, it is prudent to pay more attention to interventions in this age group in order to increase their knowledge and correct their attitude toward illegal drugs and strengthening their confidence in this regard. These interventions can have an important role in decreasing the rate of drug abuse in this age group and consequently in the whole community.
Making Radical Change Happen Through Selective Inclusion and Exclusion of Stakeholders

The purpose of this paper is to explore how political leaders can gain acceptance for radical reforms through selective inclusion and exclusion of stakeholders. We had inside access to the formulation of a radical reform in the Scandinavian context and found that radical change was not secured through extensive participation, but rather through selective inclusion and exclusion of stakeholders. Change leaders cannot always choose who to include and exclude in participative processes and both proponents and opponents can influence the content in ways that make it less radical. In the context of the public sector, it becomes particularly challenging to gain acceptance for radical reforms because of the influence from multiple stakeholders, many of whom have the right to be included in the process. Based on a study of a Norwegian regulatory reform, we identify five tactics change leaders can use to manage selective inclusion and exclusion. These include deleting stages in the process, including new stakeholders, alternating the process sequence, suppressing issues, and controlling the kind of influence. By consciously including and excluding stakeholders, change leaders can secure that the radicalness of a reform is preserved in the formulation process.
Working Backwards: Instrumental analysis as a policy discovery procedure

Following up on suggestions of Adolph Lowe, the paper draws on the work of Peirce, Polya and Michael Polanyi to elaborate the notion of Lowe's instrumental analysis as a policy discovery procedure. It is argued that such an interpretation of Lowe's instrumentalism may contribute to the formulation of effective practical policies. It is also argued in the paper that this interpretation throws light on some issues concerning markets and planning that relate to the debate on socialist calculation that has been revived in the name of the 'knowledge problem' by contemporary Austrian economists. In particular, it is argued that Lowe's Instrumentalism brings to the fore the role of discovery and creativity-which are central to Austrian conceptions of entrepreneurial activity in the market-in policy formulation. In this sense Lowe's work may be seen as an antecedent to more recent work in planning that critiques-and promotes nonessentialist alternatives to-optimal or rational planning.
Use of the WECC WAMS in Wide-Area Probing Tests for Validation of System Performance and Modeling

During 2005 and 2006, the western electricity coordinating council (WECC) performed three major tests of western system dynamics. These tests used a wide-area measurement system (WAMS) based primarily on phasor measurement units (PMUs) to determine response to events including the insertion of the 1400-MW Chief Joseph braking resistor, probing signals, and ambient events. Test security was reinforced through real-time analysis of wide-area effects, and high-quality data provided dynamic profiles for interarea modes across the entire western interconnection. The tests established that low-level optimized pseudo-random plusmn20 -MW probing with the pacific DC intertie (PDCI) roughly doubles the apparent noise that is natural to the power system, providing sharp dynamic information with negligible interference to system operations. Such probing is an effective alternative to use of the 1400-MW Chief Joseph dynamic brake, and it is under consideration as a standard means for assessing dynamic security.
Crystal Structure of the Outer Membrane Protein RcsF, a New Substrate for the Periplasmic Protein-disulfide Isomerase DsbC*

The bacterial Rcs phosphorelay is a stress-induced defense mechanism that controls the expression of numerous genes, including those for capsular polysaccharides, motility, and virulence factors. It is a complex multicomponent system that includes the histidine kinase (RcsC) and the response regulator (RcsB) and also auxiliary proteins such as RcsF. RcsF is an outer membrane lipoprotein that transmits signals from the cell surface to RcsC. The physiological signals that activate RcsF and how RcsF interacts with RcsC remain unknown. Here, we report the three-dimensional structure of RcsF. The fold of the protein is characterized by the presence of a central 4-stranded β sheet, which is conserved in several other proteins, including the copper-binding domain of the amyloid precursor protein. RcsF, which contains four conserved cysteine residues, presents two nonconsecutive disulfides between Cys74 and Cys118 and between Cys109 and Cys124, respectively. These two disulfides are not functionally equivalent; the Cys109–Cys124 disulfide is particularly important for the assembly of an active RcsF. Moreover, we show that formation of the nonconsecutive disulfides of RcsF depends on the periplasmic disulfide isomerase DsbC. We trapped RcsF in a mixed disulfide complex with DsbC, and we show that deletion of dsbC prevents the activation of the Rcs phosphorelay by signals that function through RcsF. The three-dimensional structure of RcsF provides the structural basis to understand how this protein triggers the Rcs signaling cascade.