Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of Mortality from Peptic Ulcer Bleed between Patients with or without Peptic Ulcer Antecedents

We studied mortality related to peptic ulcer bleed in a well defined cohort of patients in the month after the episode of peptic ulcer bleed. Our objective was to assess the contribution of peptic ulcer antecedents and other predictive factors on the risk of dying. The study cohort comprised 1,020 patients hospitalized for an episode of peptic ulcer bleed between January 1991 and March 1994 and identified in the General Practice Research Database in the United Kingdom. Six hundred twenty-three patients had no prior episode of peptic ulcer disease, and 384 had peptic ulcer antecedents; for 13 patients, information was not available. Forty-five patients died (mortality rate = 4.4 per 100 person-months; 95% confidence interval = 3.3–5.9) within 1 month of the peptic ulcer bleed. Patients with no peptic ulcer antecedent faced a greater risk of dying than patients with antecedents (relative risk = 3.0; 95% confidence interval = 1.2–7.1). Elderly patients, those undergoing surgery, and current users of acid-suppressing drugs or nonsteroidal antiinflammatory drugs all had an increased mortality risk. Patients presenting with their first-ever episode of peptic ulcer bleed have a higher case fatality rate than those with previous episodes of peptic ulcer. (Epidemiology 1998;9:452–456)
Comparison of the enzymatic properties of the Na,K-ATPase alpha 3 beta 1 and alpha 3 beta 2 isozymes.

The coexpression of multiple isoforms of the alpha and beta subunits of the Na,K-ATPase in mammalian tissues gives rise to the complex molecular heterogeneity that characterizes the Na pump. The expression of the different Na,K-ATPase isoforms in insect cells using recombinant baculoviruses represents a useful system for the analysis of Na,K-ATPase isoform function. In the present study, we use this system to direct the expression of the rat Na,K-ATPase alpha 3 beta 1 and alpha 3 beta 2 in sf-9 cells, a cell line derived from the ovary of the fall armyworm, Spodoptera frugiperda. The association of alpha 3 with either beta 1 or beta 2 results in catalytically competent Na,K-ATPase isozymes. Analysis of the kinetic characteristics of these enzymes demonstrates that the accompanying beta subunit isoform does not drastically affect the properties of the alpha 3 polypeptide. This is evidenced by the similar turnover numbers, apparent affinities for K+ and ATP, and the comparable high sensitivity to ouabain exhibited by both isozymes. The kinetic dependence on Na+, however, is different for both isozymes, with alpha 3 beta 2 displaying a 1.6-fold higher apparent affinity for the cation than alpha 3 beta 1. Comparison with other Na,K-ATPase isozymes shows that the apparent Na+ affinity of alpha 3 beta 2 is similar to that of the alpha 1 beta 1 Na pump widely expressed in every tissue; nevertheless, its reactivity toward K+, ATP, and ouabain are characteristic of the alpha 3 isoform. The most pronounced kinetic differences in Na,K-ATPase function are a result of variations in alpha isoform composition. (ABSTRACT TRUNCATED AT 250 WORDS)
Defective platelet aggregation in polycythaemia vera is not caused by impaired calcium signaling, phospholipase D activation or decreased amounts of focal adhesion proteins

Abstract: We have previously demonstrated that platelets in polycythaemia vera (PV) exhibit decreased aggregation after stimulation with platelet activating factor (PAF) and reduced expression of GPIIIa on both resting and stimulated platelets. In the present study, we investigated if these results were related to changes in the mobilization of intracellular calcium, activation of phospholipase D (PLD) or amounts of GPIIIa and the intracellular tyrosine kinases Fak, Syk, Grb2, Shc and rhoA. Intracellular calcium levels were not different in resting platelets from 14 PV patients and 15 healthy controls (median 43 nmol/L, range 10–114, vs. 36 nmol/L, range 10–119). After stimulation with PAF (1 µmol/L) an equal increase was seen (125 nmol/L for PV platelets, range 67–257, vs. 113 nmol/L for controls, range 60–250). Also formation of phosphatidyl ethanol (PEt) was similar after exposure to 0.5 U/ml thrombin (0.28% PEt of total phospholipid, range 0.16–1.10, vs. 0.24 for controls, range 0.11–2.3) and 1 µmol/L PMA (0.25, range 0.16–0.32, vs. 0.14, range 0.09–0.6). In contrast to the reduced amount of GPIIIa on the surface of PV platelets, immunoblotting on whole cell lysates showed no reduction in PV patients compared to controls, indicating the possibility of an impaired incorporation of GPIIIa to the cell membrane. Levels of Fak, Syk, Shc, Grb2 and rhoA appeared equal in patients and controls. Similar intracellular proteins were tyrosine phosphorylated after stimulation with thrombin, PAF and PMA. In summary, defective platelet aggregation after stimulation with PAF is caused by neither defective mobilization of intracellular calcium nor, in contrast to the situation in PV granulocytes, an impaired activation of PLD. Moreover, no apparent differences in the intracellular amounts of Fak, Syk Shc, Grb2 and rhoA could be detected between PV and control platelets.
Clonal organization in the postnatal mouse central nervous system is prefigured in the embryonic neuroepithelium.

We have used the LaacZ clonal method of cell labeling of neuronal ancestors and report that the spatial organization of neuronal cells in the post-natal CNS shares striking similarities to that in the embryonic neuroepithelium, from the spinal cord to the diencephalon. The maintenance of the organization occurs despite massive cell divisions and morphogenetic movements. We deduce that the cellular and architectural organization in the mouse CNS results from a succession of patterns of oriented cell dispersion, a general arrest of cell dispersion in the neuroepithelium, and then well-documented radial neuronal migration. The arrest of cell dispersion in the neuroepithelium is consistent with the possibility that an important part of the cellular and architectural organization of the mature CNS requires conservation of spatial relationship between cells and supports the hypothesis of a transition from global and sparse to local and dense cell interactions occurring early within the neuroepithelium.
HLA ANTIGENS AND THYROID AUTOANTIBODIES IN PATIENTS WITH GRAVES' DISEASE AND THEIR FIRST DEGREE RELATIVES

Patients with Graves' disease (n= 105) had an increased frequency of HLA‐B8 (40%) and a reduced frequency of HLA‐B12 (24·8%) when compared with random controls (n=117; 24·8% and 40·2% respectively). Comparison of patients with their first degree relatives (n= 118) shows the frequency deviations in these antigens to be characteristic of the families from which patients with Graves' disease are drawn, rather than of the disease itself. The haplotypes, identified in eighty‐six patients and 113 relatives, indicate that the excess of HLA‐B8 in patients and their relatives is primarily due to the haplotype 1–8. The relative risk for an HLA‐B8 individual of developing Graves' disease is 2·02, whilst the relative risk for an individual of haplotype 1–8 is 4·23. No significant associations were found between the incidence of any HLA antigen or combination thereof and the presence or absence of thyroglobulin and thyroid microsomal antibodies, or antibodies which interact with the TSH receptor.
Reciprocal Overshadowing between Intra-Maze and Extra-Maze Cues

In three experiments, rats learned a maze discrimination where the location of food was defined either by reference to extra-maze cues alone, or by both extra- and intra-maze cues. Experiment 1 confirmed earlier results in showing that the presence of intra-maze cues failed to overshadow learning about extra-maze cues, in spite of the former's apparently greater salience. Experiment 2, however, suggested that this result was an artefactual consequence of differences between groups in the proportion of reinforced and unreinforced trials during the course of discriminative training. In Experiment 3, the discrimination was taught by a series of reinforced and unreinforced placement trials, and a significant overshadowing effect was observed. Intra-maze and extra-maze cues seem to compete for association with reinforcement in exactly the same way as any other cues.
Differences in young people's reports of sexual behaviors according to interview methodology: a randomized trial in India.

OBJECTIVES We compared reports of sexual behaviors given in standard face-to-face interviews with reports given in audio computer-assisted self-interviews (ACASIs) and culturally specific interactive interviews among adolescents in India. We sought to determine which of the interview methods leads to higher reporting of sexual behaviors among economically disadvantaged 15-19-year-olds in urban India. METHODS We conducted a randomized trial in which each participant (583 boys and 475 girls) was assigned to 2 interview methods: face-to-face interview and ACASI or interactive interview. We used matched case-control analyses to assess differences in the individual's reporting on the 2 methods. RESULTS Female participants consistently reported fewer sexual behaviors in ACASIs than in face-to-face interviews, whereas male participants' reports differed according to type of sexual behavior and interview mode. Both male and female participants reported more sexual behaviors during interactive interviews than during face-to-face interviews. Twenty-eight percent of male participants reported having engaged in heterosexual intercourse in interactive interviews, as compared with 20% in face-to-face interviews (P< .01); the corresponding percentages for female participants were 7% and 2% (P<.01). CONCLUSIONS Our results showed that young people were more likely to report sexual behaviors in culturally specific interactive interviews than in face-to-face interviews. By contrast, ACASIs did not uniformly lead to higher reporting levels than did face-to-face interviews.
The risk of renal impairment in hormone‐refractory prostate cancer patients with bone metastases treated with zoledronic acid

Bisphosphonates have been used to treat bone metastases in hormone‐refractory prostate cancer (HRPC), but certain agents have been associated with renal toxicity. For this observational study, the authors assessed the risk of renal impairment in patients with HRPC who received zoledronic acid from December 1999 to April 2005.
PCR for Detection of Herpes Simplex Virus in Cerebrospinal Fluid: Alternative Acceptance Criteria for Diagnostic Workup

ABSTRACT The determination of herpes simplex virus (HSV) infection using a PCR assay is one of the most commonly requested tests for analysis of cerebrospinal fluid (CSF), although only a very low proportion of results are positive. A previously reported study showed that selecting only those CSF samples with >5 leukocytes/mm3 or a protein level of >50 mg/dl was adequate for the diagnostic workup. The aim of the present study was to assess the reliability of alternative acceptance criteria based on elevated CSF white blood cell counts (>10 cells/mm3). We analyzed all requests for HSV PCR received between January 2008 and December 2011. CSF samples were accepted for analysis if they had >10 cells/mm3 or if the sample was from an immunocompromised patient or a child aged <2 years. In order to evaluate our selection criteria, we identified those CSF samples with a leukocyte count of 5 to 10 cells/mm3 or protein levels of >50 mg/dl in order to test them for HSV type 1 and 2 (HSV-1 and HSV-2) DNA. During the study period, 466 CSF samples were submitted to the microbiology laboratory for HSV PCR. Of these, 268 (57.5%) were rejected, and 198 (42.5%) were tested according to our routine criteria. Of the tested samples, 11 (5.5%) were positive for HSV DNA (7 for HSV-1 and 4 for HSV-2). Of the 268 rejected specimens, 74 met the criteria of >5 cells/mm3 and/or protein levels of >50 mg/dl. Of these, 70 (94.6%) were available for analysis. None of the samples yielded a positive HSV PCR result. Acceptance criteria based on CSF leukocyte counts, host immune status, and age can help to streamline the application of HSV PCR without reducing sensitivity.
Costimulatory Pathways in Multiple Sclerosis: Distinctive Expression of PD-1 and PD-L1 in Patients with Different Patterns of Disease1

T lymphocytes costimulatory molecules, including CD80, CD86, CD28, CTLA4, PD-1, PD-L1, and B7-H3, are associated with the preferential production of pro- or anti-inflammatory cytokines. We analyzed the expression of these molecules and myelin basic protein (MBP)-specific IL-10 and IFN-γ production in patients with multiple sclerosis (MS) with relapsing-remitting acute (AMS, n = 40) or stable (SMS, n = 38). Twenty-two patients successfully undergoing therapy with glatimer acetate (n = 12) or IFNβ (n = 10) were also analyzed. MBP-specific and PD-1-expressing T lymphocytes, PD-L1-expressing CD19+ cells, and PD-L1+/IL-10+/CD14+ and CD19+ cells were significantly augmented in SMS patients. Additionally, MBP-specific and annexin V-expressing CD4+ and CD8+ (apoptotic) T lymphocytes were augmented and pAkt-positive (proliferating) cells were decreased in SMS compared with AMS patients. PD-1 ligation resulted in the increase of pAkt+ lymphocytes in AMS patients alone. B7-H3 expression and IFN-γ production were comparable in all individuals but the PD-L1+/IL-10+ over B7-H3+/IFN-γ+ ratio was significantly lower in AMS compared with SMS patients. Finally, PD-L1 expression on immune cells was reduced in treated patients, suggesting that therapy-induced disease remission is not associated with the modulation of the expression of this molecule. The PD-1/PD-L1 pathway plays an important role in modulating immune functions in MS patients; monitoring and targeting these proteins could offer diagnostic and therapeutic advantages.
Influence of Test Distance on Change of Direction Speed Test Results

Abstract Sayers, MGL. Influence of test distance on change of direction speed test results. J Strength Cond Res 29(9): 2412–2416, 2015—This study assessed the relationships between linear running velocity and change of direction (CoD) ability and how assessing CoD ability over distances ⩽5 m influences test reliability. Participants (n = 15) from amateur rugby league teams performed 3 trials of a 20-m sprint test (light gates at 5, 10, and 20 m) and 6 trials of the 5-0-5 agility test. Twelve participants repeated the 5-0-5 test several weeks later. A three-dimensional motion capture system (250 Hz) was used to track the center of mass at 0.3, 0.5, and 1.0 m either side of the turn and identify specific CoD phase times. Pearson's correlations showed strong significant relationships between the 5-0-5 time and 5-m (r = 0.89, p < 0.001), 10-m (r = 0.91, p < 0.001), and 20-m sprint times (r = 0.93, p < 0.001). However, the strength of these relationships decreased (r < 0.65, p > 0.05) when CoD ability was measured over distances less than 0.5 m. Analysis of coefficient of variation (CV%) data indicated that the 5-0-5 test had high intratest (CV% = 2.8) and intertest reliability (CV% = 1.3), with these data decreasing for distances less than 1 m (CV% = 3.5–6.9). Specific movement phase times were the least reliable measures of CoD ability (CV% = 4.7–53.6). Results suggest a bias between high-speed linear running ability and 5-0-5 time. However, an effective compromise can be found between test reliability and the external validity by assessing CoD ability over 1 m. Findings indicate that the current practice of assessing CoD ability over large distances in questionable.
Interaction of Ap1, Ap2, and Sp1 with the regulatory regions of the human pro-alpha1(I) collagen gene.

In the pro-alpha1(I) collagen gene a number of cis-regulatory elements, which interact with a variety of trans-acting factors, are present in the promoter and first intron. We have undertaken a comprehensive study of Sp1, Ap1, and Ap2 binding in the region spanning -442 to +1697 nt. DNase I footprinting analysis revealed these factors bind with varying affinities to some of the potential sites: Sp1 binds to 16 of 34 potential sites, Ap2 binds to 22 of 40 potential binding sites, and Ap1 binds to its only potential site. The Sp1 sites were mostly clustered in the intron region, while the Ap2 sites were clustered in the promoter region. Transmission electron microscopic analysis of DNA-protein complexes not only confirmed these results, but also clearly showed that heterologous and/or homologous protein-protein interactions between Sp1 and/or Ap2 bring the promoter and intron in contact with each other, with the resulting looping out of the intervening DNA. This strongly suggests that the DNA-looping model is an explanation for the orientation preference of the enhancing element in the first intron as these interactions possibly create an optimum environment for the binding of the rest of the transcriptional machinery.
Performance and ileal histomorphology of rats treated with humic acid preparations.

As humic acid (HA) substances have antiphlogistic, adsorptive, antitoxic and antimicrobial properties, we studied the possible effects of Farmagülatör, an organic HA preparation, on the rat performance, nutrient retention, ileal histomorphology and hydroxyproline (HP) content of the ileum in two experiments. In experiment 1, 48 male Wistar albino rats were allotted to three dietary treatments. Each was randomly assigned to four cages, each with four rats. The treatments consisted of a control diet (C) with no addition of Farmagülator Dry or Liquid, a treatment with addition of 2.5 g/kg Farmagülator Dry (FDry) and a control diet containing no FDry, but the rats had 3.5 ml/l Farmagülator Liquid in drinking water (FLiquid). The experiment lasted for 20 days. Changes in live weight were recorded at days 10 and 20 of the experiment. At the end of 20 days, all rats were killed to collect samples of intestinal tissues for the measurements of histological parameters. In experiment 2, 30 rats weaned at 21 days of age were divided into three groups, each with 10 rats, and individually caged in metabolism cages for 10 days. The above three treatments were randomly assigned to rats for 10 days to record body weight and feed intake. During the last 5 days, faecal outputs were collected to determine the dry matter and nitrogen retention. In experiment 1, FDry and FLiquid rats significantly (p<0.05) gained more live weight than the control rats. Improved weight gain with HA preparations was found to be highly associated with a high epithelial surface area as there were significantly (p<0.05) longer villi heights and crypt depths and increased HP contents of ileum in the HA treated rats compared with the control rats. Although the increased weight gain in FLiquid rats did not significantly (p>0.05) differ from the control rats in experiment 2 in contrast to the result in experiment 1, the FDry rats significantly (p < 0.05) gained more weight than the control rats. This was primarily found to be associated with significantly (p<0.05) increased feed intake and nitrogen retention in FDry rats compared with the control rats. It can be concluded that HA preparations, especially FDry, caused increased weight gain in rats as overall of two experiments. The improved weight gain only by FDry preparation was associated with increased ileal epithelial mass, increased feed intake, improved feed : gain ratio and increased nitrogen retention in rats.
Parental bonding and personality in relation to a lifetime history of depression

This study explores whether personality is mediating the effects of adverse parenting on having had a lifetime history of major depressive disorder and whether personality dimensions, related to the development of lifetime depression, are disposed by adverse parenting in cross‐sectional data derived from an epidemiological sample of volunteer workers. Of 447 individuals who were asked to complete the Munich Personality Test (MPT), the Parental Bonding Instrument (PBI) and the Inventory to Diagnose Depression Lifetime version (IDDL), 322 subjects were included in the analyses (150 male and 172 female; and 38 were diagnosed as having had a history of depression). Comparisons in fit between logistic regression models revealed that a combination of frustration tolerance and rigidity among personality dimensions, as measured by the MPT, and maternal care among the PBI scales were most primary in predicting a lifetime history of depression. Maternal care was, however, not significantly predictive of dimensional scores on the personality dimensions. Neither frustration tolerance nor rigidity was predicted by any PBI scale. When entering the variables sequentially, maternal care and the personality variables were additive and independent risk factors in predicting a lifetime history of depression. The results of this preliminary study raised an objection to a hypothesis that adverse parenting experienced in childhood disposes one to a dysfunctional personality, which then predisposes one to the development of depression in adulthood.
Representative bureaucracy: does female police leadership affect gender-based violence arrests?

Representative bureaucracy theory postulates that passive representation leads to active representation of minority groups. This article investigates the passive representation of female police officers at leadership levels and the active representation of women vis-a-vis gender-based violence arrest rates in the UK. Much of the extant research on representative bureaucracy is located at street level, with evidence showing that discretionary power of minority bureaucrats can lead to active representation. This article is focused on leadership levels of a public bureaucracy. The empirical research is based upon a panel dataset of female police officers as an independent variable and gender-based violence arrest rates as a dependent variable. The analysis reveals that there is little evidence of active representation of women by female police leadership. Points for practitioners The research offers policy and public service practitioners with empirical evidence to suggest that representation of minorities is mediated by organizational effects in public bureaucracies.