Miracle-dz/UniFAR-FTUs huggingface dataset:
Etude de la nébulisation de téicoplanine en ventilation mécanique

La teicoplanine est utilisee par voie intraveineuse (IV) dans le traitement de la pneumonie acquise sous ventilation mecanique (PAVM) a Staphylocoque aureus meticilline resistant. Nous avons evalue la faisabilite et les benefices de la nebulisation de teicoplanine en ventilation mecanique (VM) par rapport a la voie IV. L’administration d’un aerosol de teicoplanine chez le rat a permis d’obtenir des concentrations pulmonaires 48 fois superieures a celles apres IV et des concentrations plasmatiques inferieures a celles apres IV. Apres avoir mesure l’influence des nebuliseurs sur les parametres ventilatoires du respirateur, nous avons selectionne le nebuliseur Aeroneb Pro®. Les proprietes bacteriologiques de l’antibiotique ne sont pas alterees par le nebuliseur et l’exposition d’un modele d’epithelium pulmonaire in vitro a des doses 100 fois superieures a celles utilisees en IV n’a pas montre de cytotoxicite. L’etude realisee sur la nebulisation de teicoplanine chez le porc en VM a montre un depot pulmonaire moyen de 18,1%. En conclusion, la nebulisation de teicoplanine pourrait etre envisagee comme une nouvelle arme therapeutique dans la PAVM.
PENGARUH E-SERVICE QUALITY TERHADAP E-CUSTOMER SATISFACTION WEBSITE START-UP KAOSYAY

Pertumbuhan pesat pangsa pasar e-commerce di Indonesia memang sudah tidak bisa diragukan lagi. Untuk menghadapi intensitas tantangan yang semakin berat, perusahaan dalam menjalankan bisnisnya dituntut berfikir kreatif untuk menemukan berbagai terobosan strategi yang mampu menciptakan sinergi yang memberikan kontribusi optimal bagi pencapaian tujuan perusahaannya. Oleh karena itu, maka penting diadakannya penelitian untuk mengetahui bagaimana pengaruh kualitas layanan terhadap kepuasan pelanggan pada pelanggan situs berbelanja online kaosyay.com Penelitian ini dilakukan untuk mengukur penilaian pelanggan terhadap variabel e-service quality dan e-customer satisfaction. Selain itu, penelitian ini bertujuan untuk mengevaluasi pengaruh variabel e-service quality dan e-customer satisfaction. Jenis penelitian yang digunakan pada penelitian ini adalah deskriptif kausal. Item kuesioner yang digunakan sebanyak 28 item. Pengumpulan data dilakukan dengan cara penyebaran kuesioner kepada pengguna dari situs berbelanja online kaosyay.com yang telah mengakses kaosyay dengan jumlah sampel sebanyak 30 orang yang ditentukan dengan metode nonprobability purposive sampling. Teknik analisis data yang digunakan adalah Structural Equation Model dengan tingkat signifikansi 5%. Hasil yang diperoleh pada penelitian ini adalah e-service quality berpengaruh positif dan signifikan terhadap e-customer satisfaction. Adapun saran bagi perusahaan adalah dengan meningkatkan e-service quality baik dari segi manajemen dan infrastruktur, lebih memperhatikan proses pelayanan kepada pelangggan, dan meningkatkan pelayanannya. Saran bagi peneliti selanjutnya adalah dapat menyempurnakan dengan teori dan data yang lebih baik dan penelitian tidak hanya pada sektor e-commerce tapi juga pada bidang bisnis berbasis teknologi lainnya.
Acetaldehyde Disrupts Interferon Alpha Signaling in Hepatitis C Virus-Infected Liver Cells by Up-Regulating USP18.

BACKGROUND Alcohol consumption exacerbates the pathogenesis of hepatitis C virus (HCV) infection and worsens disease outcomes. The exact reasons are not clear yet, but they might be partially attributed to the ability of alcohol to further suppress the innate immunity. Innate immunity is known to be already decreased by HCV in liver cells. METHODS In this study, we aimed to explore the mechanisms of how alcohol metabolism dysregulates IFNα signaling (STAT1 phosphorylation) in HCV+ hepatoma cells. To this end, CYP2E1+ Huh7.5 cells were infected with HCV and exposed to the acetaldehyde (Ach) generating system (AGS). RESULTS Continuously produced Ach suppressed IFNα-induced STAT1 phosphorylation, but increased the level of a protease, USP18 (both measured by Western blot), which interferes with IFNα signaling. Induction of USP18 by Ach was confirmed in primary human hepatocyte cultures and in livers of ethanol-fed HCV transgenic mice. Silencing of USP18 by specific siRNA attenuated the pSTAT1 suppression by Ach. The mechanism by which Ach down-regulates pSTAT1 is related to an enhanced interaction between IFNαR2 and USP18 that finally dysregulates the cross talk between the IFN receptor on the cell surface and STAT1. Furthermore, Ach decreases ISGylation of STAT1 (protein conjugation of a small ubiquitin-like modifier, ISG15, Western blot), which preserves STAT1 activation. Suppressed ISGylation leads to an increase in STAT1 K48 polyubiquitination which allows pSTAT1 degrading by proteasome. CONCLUSIONS We conclude that Ach disrupts IFNα-induced STAT1 phosphorylation by the up-regulation of USP18 to block the innate immunity protection in HCV-infected liver cells, thereby contributing to HCV-alcohol pathogenesis. This, in part, may explain the mechanism of HCV-infection exacerbation/progression in alcohol-abusing patients.
Antigen Analysis of Mycobacterium tuberculosis H37Rv Culture Filtrate Proteins

Culture filtrates of Mycobacterium tuberculosis H37Rv highly enriched with secreted proteins were used to identify antigens recognized by a serum pool from tuberculosis patients. Two different approaches were used to separate the culture filtrate protein mixture: (i) proteins were fractionated according to their hydrophobicity using an HPLC‐C18 chromatography column followed by separation based on their molecular mass by SDS‐PAGE and subsequent immunoblotting or (ii) proteins were separated by two‐dimensional gel electrophoresis, based on their isoelectric point and their molecular mass. Twenty serologically reactive proteins were ultimately identified by both methods, including four novel antigens. Further, to estimate the immunogenicity of the identified culture filtrate proteins, the relative antibody quantities were measured using Image master software. Our results show that the antibodies against proteins belonging to the antigen 85 complex were the most abundant in the serum of patients with active tuberculosis. The most immunogenic proteins in terms of high antibody‐to‐protein‐ratio were Rv3881c and three lipoproteins Rv0934 (the 38 kDa antigen), Rv0932c (pstS2), and Rv3006 (LppZ). Rv3881c is located in the region of difference 1 (RD1) which is deleted from Mycobacterium bovis BCG, and is therefore a particularly promising candidate for development of serodiagnostic assays to detect active tuberculosis. The proteins from the M. tuberculosis H37Rv culture filtrate are strong candidates to be evaluated for improvement of the serodiagnostic tests of tuberculosis.
Controlling coach style, basic psychological need thwarting and ill-being in soccer players

Grounded on basic psychological needs theory the aim of this paper was to test a model following the sequence: perceived coach controlling interpersonal style - basic psychological needs thwarting – player feelings of discomfort. 433 young male football players (Mage = 13.58 ± 0.56) responded to the variables of interest. Structural equation modeling results showed that perceived controlling interpersonal style positively predicted the players’ basic psychological needs thwarting, which in turn positively predicted their burnout, their negative affects and their symptoms of physical and psychological discomfort. Indirect effects of coach controlling interpersonal style on feelings of disaffection indicators through basic psychological needs thwarting, were also found. The results compel us to think about the adverse consequences of the controlling contexts created by authority figures over young football players.
An Eco-Epidemiological Model with Different Competition Coefficients and Strong-Allee in the Prey

Strong-Allee effect and competition coefficients provide useful insight to the dynamics of eco-epidemiological systems. Basically, different competition coefficients in the prey population lead to the emergent carrying capacity. The study of population dynamics under the influence of strong-Allee parameter has attracted a lot attention from researchers. But the dynamics of such systems under the combined effect of disease, Allee and emergent carrying capacity is yet to receive attention from researchers. A simple eco-epidemiological model with strong-Allee effect in prey population is proposed and analyzed with the aim to observe the dynamics of such system under the combined influence of strong-Allee parameter and competition coefficients. The basic mathematical features of the model are analyzed with the help of equilibrium analysis, stability analysis, bistability and bifurcation theory. Our numerical simulations reveal that in the absence of strong-Allee effect, the three species eco-epidemiological system produces chaos by increasing the force of infection. However, we observe that chaotic dynamics thus obtained can be controlled by the Allee parameter as well as the competition coefficients.
EFFECT OF SOIL SALINITY ON GROWTH , GAS EXCHANGE AND ANTIOXIDANT DEFENSE OF TWO PAULOWNIA LINES

This study was conducted to assess the tolerance of two Paulownia hybrid lines (P. tomentosa x fortunei – TF01 and P. elongata x elongata T4) to salt stress based on some physiological and biochemical parameters at the vegetative stage. The effect of salinity on gas exchange and activities of superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6) and guaiacol peroxidase (GPOX, EC 1.11.1.7) in two year-aged Paulownia plants (P. tomentosa x fortunei TF01 and P. elongata x elongata T4) grown on different types of soil for a period of four months was investigated. The soil samples were collected from two areas of the village Belozem near Plovdiv city, Bulgaria. The sodium adsorption ratio (SAR) for the first and second type of soil was 0.187 and 0.856, respectively. It was found that the increase of soil salinity led to a more pronounced reduction of total dry biomass, leaf area, the total leaf area/leaf number ratio as well as the leaf K/Na ratio in TF01 than in T4. Salinity significantly enhanced the level of malondialdehyde (MDA) and hydrogen peroxide (H2O2), as well as the activities of SOD, CAT and GPOX in TF01. The increased enzyme activities in TF01 suggested that they might play a possible role in the tolerance of this line to salinity. With increasing salinity level, the net photosynthetic rate (PN) and stomatal conductance (Gs) were reduced, while transpiration rate (E) increased insignificantly in both lines. Respiration rate (Rd) was enhanced in T4, but not in TF01. Water use efficiency (WUE) was not improved at high salinity level and the decreasing trend was more pronounced in TF01 than in T4. It was evident that soil salinity affected gas exchange, water relations and antioxidant defense in both lines in a different way.
Modified General Primer PCR System for Sensitive Detection of Multiple Types of Oncogenic Human Papillomavirus

Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer system GP5+/6+ using a proficiency panel with HPV plasmid dilutions as well as cervical samples from 592 women with low-grade cytological abnormalities. The reference method (GP5+/6+) had the desirable high sensitivity (five copies/PCR) for five oncogenic HPV types (HPV type 16 [HPV-16], HPV-18, HPV-56, HPV-59, and HPV-66). The MGP system was able to detect all 14 oncogenic HPV types at five copies/PCR. In the clinical samples, the MGP system detected a significantly higher proportion of women with more than two concomitant HPV infections than did the GP5+/6+ system (102/592 women compared to 42/592 women). MGP detected a significantly greater number of infections with HPV-16, -18, -31, -33, -35, -39, -42, -43, -45, -51, -52, -56, -58, and -70 than did GP5+/6+. In summary, the MGP system primers allow a more sensitive amplification of most of the HPV types that are established as oncogenic and had an improved ability to detect multiple concomitant HPV infections.
Chiasma Frequency Is Region Specific and Chromosome Conformation Dependent in a Rye Chromosome Added to Wheat

In many plant species synapsis starts at, or close to, the chromosome ends and this has been considered to be related to the distal location of chiasmata. In this regard we have studied the meiotic behavior of rye chromosome pair 5R in a wheat background using fluorescence in situ hybridization. The use of different DNA probes allowed the identification of the 2 rye homologues, their centromeres and subtelomeric heterochromatic chromomeres, and the telomeres of all chromosomes in prophase I and metaphase I. Three types of plants were analyzed: homozygotes for the standard chromosome 5R, homozygotes for a deficient chromosome 5R (del5R) with only the proximal 30% of its long arm (del5RL) and heterozygotes. Synapsis of the deficient chromosome arm pair del5RL was completed in most meiocytes at pachytene but the number of chiasmata formed was much lower than in the intact 5RL arm. Deletion facilitated the migration of the telomere of the accompanying chromosome arm 5RS during bouquet organization. This was followed by an increase of synapsis and chiasma frequency in this arm with regard to its counterpart of the intact chromosome. Results demonstrate that crossover formation depends on the DNA sequence or the chromatin organization of each chromosome region and that homologous alignment, synapsis and chiasma formation may be conditioned by chromosome conformation.
A Comparison of Adaptive Kalman Filters for a Tropical Pacific Ocean Model

The Kalman filter is the optimal linear assimilation scheme only if the first- and second-order statistics of the observational and system noise are correctly specified. If not, optimality can be reached in principle by using an adaptive filter that estimates both the state vector and the system error statistics. In this study, the authors compare the ability of three adaptive assimilation schemes at estimating an unbiased, stationary system noise. The adaptive algorithms are implemented in a reduced space linear model for the tropical Pacific. Using a twin experiment approach, the algorithms are compared by assimilating sea level data at fixed locations mimicking the tropical Pacific tide gauges network. It is shown that the description of the system error covariance matrix requires too many parameters for the adaptive problem to be well posed. However, the adaptive procedures are efficient if the number of noise parameters is dramatically reduced and their performance is shown to be closed to optimal, that is, based on the true system noise covariance. The link between those procedures is elucidated, and the question of their applicability and respective computational cost is discussed.
Sino‐aortic denervation in the monkey.

The aortic arch and carotid sinuses were denervated in eleven monkeys. The monkeys were subjected to four sequential surgeries which involved: (1) implantation of an aortic and left atrial catheter; (2) stripping of the adventitia from the aortic arch; (3) stripping the left carotid sinus and associated vessels; and (4) stripping the right carotid sinus and associated vessels. Blood pressure and pulse rate were recorded 6 days after each surgical procedure. Records were taken over a 6 h period while the monkeys were in their home cages. Baroreceptor denervation was confirmed by: (1) absence of heart rate response to blood pressure changes and (2) an increase in the variability of blood pressure. Veratridine given into the left atrium caused a Bezold‐Jarisch reflex both before and after denervation verifying the integrity of the afferent and efferent vagus. Denervation of the baroreceptors resulted in a significant increase in blood pressure when measured from monkeys who were restrained in chairs in the laboratory; however, blood pressure was not significantly elevated in the baroreceptor denervated monkeys while they were tethered in their home cages. It is concluded that denervation of the sino‐aortic baroreceptor does not result in a significant increase in systemic blood pressure.
Evaluation of a dot ELISA kit for measuring immunoglobulin M antibodies to canine parvovirus and distemper virus

A dot ELISA for the detection of immunoglobulin M (IgM) antibodies to canine distemper virus (CDV) and canine parvovirus (cpv) was assessed. The titres of IgM antibodies to CDV and cPv in 100 dogs were measured by the Immunocomb ELISA kit and compared with the results derived from the immunofluorescence assay (IFA). There was a strong correlation between the results of the dot ELISA technique and the IFA (P<0.001). The dot ELISA kit was also used to assess the changes in the levels of immunoglobulin G (IgG) and IgM antibodies to cpv and CDv in 10 puppies vaccinated with a polyvalent vaccine. High levels of IgM antibodies to cpv were first detected seven days after they were vaccinated, and after nine days all the pups had high titres of IgG antibodies to cpv. High levels of IgM antibodies to CDV were detected after nine days and the highest average titres were recorded after 12 days. IgG antibodies to CDV were present from nine days after vaccination.
Long‐Term Follow‐up of Children Born to Women Immunized With Tetanus Toxoid During Pregnancy

ABSTRACT: Ten years after transplacental immunization with tetanus toxoid, the antibody responses of the immunized children to a booster immunization with 5 Lf tetanus toxoid did not differ from those of the control children. The tetanus toxoid‐stimulated lymphocyte proliferative responses showed that there was no tolerance to tetanus toxoid induced by transplacental immunization, and the stimulation indices suggested that there may be some long‐term memory for tetanus toxoid among the T lymphocytes in children who had been transplacentally immunized by maternal immunization with a standard dose of tetanus toxoid.
Epidemiological study of the prevalence of depressive disorders in primary health care in Morocco

Objective: To determine the prevalence and the impact of depressive disorders in primary health care and its associated factors. Methodology: It's a cross-sectional study with 351 participants selected from Moroccan primary care facilities, aged above 18 years without chronic somatic or psychiatric disease. The participants answered a questionnaire that included demographic characteristics, the Mini International Neuropsychiatric Interview for major depressive episode (MDE), dysthymic disorder and the Global Assessment of Functioning (GAF). Statistical analysis was performed by the SPSS 13.0 software. Results: The prevalence of depressive disorders in the sample was 13.7%, that of MDE was 9.1%, while dysthymic disorder was 4.3%, the rate of recurrent depressive episodes was 38.2% (6% of participants), and the prevalence of depression over a lifetime was 17.7%. The percentage of depression was higher among women than men (P = 0.01). 6.3% of depressed patients have already attempted to suicide. Analysis of GAF scores showed an average of 76.2 ± 24, a lower score was significantly found among patients with current MDE (P = 0.001), dysthymic subjects (P = 0.001) and those who suffer from recurrent MDE (P = 0.001). Depressive disorders in univariate analysis were associated with: Female gender P = 0.01 odds ratio (OR) 2.1 (1.09–4.3), unemployment P = 0.02 OR 0.4 (0.2–0.9), and childbearing age P = 0.004 OR 3.5 (1.5–8). Adjusted OR has not demonstrated a significant association. Conclusion: The high prevalence of depressive disorders, suicide risk, and the alteration of the quality of life among primary health care patients in Morocco suggest the importance of identifying and treating this population.
Attitudinal Ambivalence and Message-Based Persuasion: Motivated Processing of Proattitudinal Information and Avoidance of Counterattitudinal Information

Attitudinal ambivalence has been found to increase processing of attitude-relevant information. In this research, the authors suggest that ambivalence can also create the opposite effect: avoidance of thinking about persuasive messages. If processing is intended to reduce experienced ambivalence, then ambivalent people should increase processing of information perceived as proattitudinal (agreeable) and able to decrease ambivalence. However, ambivalence should also lead people to avoid processing of counterattitudinal (disagreeable) information that threatens to increase ambivalence. Three studies provide evidence consistent with this proposal. When participants were relatively ambivalent, they processed messages to a greater extent when the messages were proattitudinal rather than counterattitudinal. However, when participants were relatively unambivalent, they processed messages more when the messages were counterattitudinal rather than proattitudinal. In addition, ambivalent participants perceived proattitudinal messages as more likely than counterattitudinal messages to reduce ambivalence, and these perceptions accounted for message position effects on amount of processing.
Circadian clock properties of fruit flies Drosophila melanogaster exhibiting early and late emergence chronotypes

ABSTRACT The role of circadian clocks in timing daily behaviors is widely acknowledged, and while empirical evidence suggests that clock period is correlated with the preferred phase of a rhythmic behavior (chronotype), other clock properties have also been hypothesized to underlie chronotype variation. Here, we report that fruit fly Drosophila melanogaster populations exhibiting evening emergence chronotype (late) are characterized by higher incidence of behavioral arrhythmicity in constant dim light, wider range of entrainment, reduced rates of re-entrainment to simulated jet-lag and higher amplitude of both entrained and free-running rhythms as compared to those exhibiting morning emergence chronotype (early). Our results thus highlight the role of circadian clock properties such as zeitgeber sensitivity, amplitude and coupling in driving chronotype variation.