Miracle-dz/UniFAR-FTUs huggingface dataset:
Adolescent Choline Supplementation Attenuates Working Memory Deficits in Rats Exposed to Alcohol During the Third Trimester Equivalent.

BACKGROUND Children exposed to alcohol prenatally may suffer from behavioral and cognitive alterations that adversely affect their quality of life. Animal studies have shown that perinatal supplementation with the nutrient choline can attenuate ethanol's adverse effects on development; however, it is not clear how late in development choline can be administered and still effectively reduce the consequences of prenatal alcohol exposure. Using a rodent model, this study examined whether choline supplementation is effective in mitigating alcohol's teratogenic effects when administered during adolescence/young adulthood. METHODS Sprague-Dawley rats were exposed to alcohol (5.25 g/kg/d) during the third trimester equivalent brain growth spurt, which occurs from postnatal day (PD) 4 to 9, via oral intubation. Sham-intubated and nontreated controls were included. Subjects were treated with 100 mg/kg/d choline chloride or vehicle from PD 40 to 60, a period equivalent to young adulthood in the rat. After the choline treatment had ceased, subjects were tested on a series of behavioral tasks: open field activity (PD 61 to 64), Morris water maze spatial learning (PD 65 to 73), and spatial working memory (PD 87 to 91). RESULTS Ethanol-exposed subjects were overactive in the activity chambers and impaired on both the spatial and the working memory versions of the Morris water maze. Choline treatment failed to attenuate alcohol-related overactivity in the open field and deficits in Morris water maze performance. In contrast, choline supplementation significantly mitigated alcohol-related deficits in working memory, which may suggest that choline administration at this later developmental time affects functioning of the prefrontal cortex. CONCLUSIONS The results indicate that adolescent choline supplementation can attenuate some, but not all, of the behavioral deficits associated with early developmental alcohol exposure. The results of this study indicate that dietary intervention may reduce some fetal alcohol effects, even when administered later in life, findings with important implications for adolescents and young adults with fetal alcohol spectrum disorders.
Have disfluency-type measures contributed to the understanding and treatment of developmental stuttering?

PURPOSE This article critically reviews evidence to determine whether the use of disfluency typologies, such as syllable repetitions or prolongations, has assisted the understanding or treatment of developmental stuttering. Consideration is given to whether there is a need for a fundamental shift in the basis for constructing measures of stuttering behavior. METHOD The history of using specific types of disfluencies to assess stuttering, including more recent developments such as counts of stuttering-like disfluencies, is reviewed. The focus is on studies that have investigated the validity and reliability of these perceptually based assessment methods. CONCLUSION The evidence from use of disfluency-type measures shows that the behavioral difference between stuttering and normally fluent speakers is solely related to the amount of observable stuttering; the differences are only partially realized within disfluency-type measures. Indeed, because disfluency-type measures show poor reliability and conflate stuttered and nonstuttered speech, they have only limited heuristic value for research and provide no obvious benefits for clinicians. At best, they should be regarded as imprecise descriptors of observable stuttering and not a fundamental measure of stuttering. A recommended solution to the problematic history of verbal-based definitions of stuttering behavior is continued development and investigation of exemplar-based definition and measurement.
Exploring Teacher Leadership in a Rural, Secondary School: Reciprocal Learning Teams as a Catalyst for Emergent Leadership.

The purpose of this case study was to examine how teachers experienced professional development as collaborative inquiry, and how their experiences contributed to their development as teacher leaders. Three overarching themes were identified through iterative qualitative analysis of multiple data sources including interviews, observations, participant reflections, and classroom artifacts. Through inquiry foci derived and developed in small learning teams, teachers were able to establish increased ownership and sense of agency towards change at the classroom and school level. The authors recommend sustained focus on (a) the emergent and fluid nature of teacher leadership experienced and fostered through collaborative inquiry; (b) attention to educators’ personal and interpersonal social and emotional competencies as an important aspect of teacher leadership; and (c) how rural and/or small secondary school contexts offer and require situated leadership development opportunities. This case study offers the field illustrations of teacher leadership that challenge typology-oriented descriptions.
Climate- and Environment-Induced Intervillage Migration in Southwestern Burkina Faso, West Africa

AbstractThis study investigated key environmental factors causing intervillage migration by farmers. Therefore, it used household data from surveys, semistructured interviews, life histories, and focus group discussions in southwestern Burkina Faso, West Africa. The results showed that 1) when referring to the experienced historical weather and climate, farmers were aware of the effects of ongoing climate and environmental change; 2) soil degradation, land tenure insecurity, and lack of rainfall were major drivers of environment-induced migration; and 3) soil fertility, productivity, rainfall, and humidity, as well as land tenure security, were major pull factors. Farmers indirectly identified population pressure as a major driver of intervillage migration since it contributes to land degradation and land tenure insecurity. It is argued that migration implicitly adds to the natural climate and environmental stresses. When aiming to elaborate suitable land-use planning, the findings call for additional res.
Twitter as a formal and informal language learning tool: from potential to evidence

Twitter can be used as a language learning tool and this potential has been identified by a number of scholars. This chapter presents an overview of the identified potential of Twitter as a language learning tool and presents an overview of different studies carried out to provide evidence of language learning using Twitter in different contexts. It concludes that, although there is evidence of language acquisition in formal contexts, more research is needed to inform how Twitter is used in informal settings.
Economic–Environmental Sustainability in Building Projects: Introducing Risk and Uncertainty in LCCE and LCCA

The aim of this paper is to propose a methodology for supporting decision-making in the design stages of new buildings or in the retrofitting of existing heritages. The focus is on the evaluation of economic–environmental sustainability, considering the presence of risk and uncertainty. An application of risk analysis in conjunction with Life-Cycle Cost Analysis (LCCA) is proposed for selecting the preferable solution between technological options, which represents a recent and poorly explored context of analysis. It is assumed that there is a presence of uncertainty in cost estimating, in terms of the Life-Cycle Cost Estimates (LCCEs) and uncertainty in the technical performance of the life-cycle cost analysis. According to the probability analysis, which was solved through stochastic simulation and the Monte Carlo Method (MCM), risk and uncertainty are modeled as stochastic variables or as “stochastic relevant cost drivers”. Coherently, the economic–financial and energy–environmental sustainability is analyzed through the calculation of a conjoint “economic–environmental indicator”, in terms of the stochastic global cost. A case study of the multifunctional building glass facade project in Northern Italy is proposed. The application demonstrates that introducing flexibility into the input data and the duration of the service lives of components and the economic and environmental behavior of alternative scenarios can lead to opposite results compared to a deterministic analysis. The results give full evidence of the environmental variables’ capacity to significantly perturb the model output.
Forecasting hotel demand uncertainty using time series Bayesian VAR models

Demand uncertainty is a fundamental characteristic of the hospitality industry. Hotel room inventory is fixed, and devising an accurate daily demand measurement is a key operational challenge. In practice, it is difficult to predict the industry stability and capture demand uncertainty, so the industry relies on demand estimates. This process of estimation affects revenue maximization, as it is sensitive to incremental costs. In this article, we implemented vector autoregressive (VAR) models and compared them to the Bayesian VAR to examine the accuracy of predicting demand. We evaluated the results using a new measure of forecasting accuracy, the mean arctangent absolute percentage error (MAAPE). The results generated from the forecasts confirm the significant improvement in forecasting performance that can be obtained using the Bayesian model. It is noteworthy that the VAR performs the best for the lower horizons. The results also suggest that MAAPE outperforms other existing accuracy measures, in terms of error rates.
Acetylcholine elicits a rebound stimulation of Ca2+ current mediated by pertussis toxin-sensitive G protein and cAMP-dependent protein kinase A in atrial myocytes.

Cholinergic inhibition of atrial contraction is typically followed by a rebound positive inotropic response. In the present study, we used a nystatin-perforated patch whole-cell recording method to determine whether acetylcholine (ACh) elicits a rebound stimulation of L-type Ca2+ current (ICa,L) in cat atrial myocytes. ACh (1 mumol/L) decreased basal ICa,L (-19 +/- 2%). Within approximately 30 s of returning to ACh-free solution, basal ICa,L exhibited a rebound increase above the control level (+61 +/- 7%) that returned to the control level within 4 to 5 minutes. ACh elicited concomitant changes in cell shortening, ie, a decrease followed by a rebound increase. The EC50 and maximal response of ACh-induced inhibition and rebound stimulation of ICa,L were 1.9 x 10(-9) mol/L and -30%, respectively, and 2.9 x 10(-8) mol/L and +64%, respectively. All effects of ACh on ICa,L were blocked by prior exposure to 1 mumol/L atropine or 100 mumol/L AFDX116 and unaffected by 0.2 mumol/L pirenzepine or 1 mumol/L propranolol. In the presence of ACh, exposure to atropine elicited stimulation of ICa,L.ACh-induced inhibition and rebound stimulation of current were independent of external Ca2+. Rebound stimulation of ICa,L was associated with a negative shift in the voltage dependence of ICa,L activation. Inhibition of protein kinase A by 50 mumol/L Rp-cAMPs decreased basal ICa,L by 36 +/- 1% and abolished the rebound stimulation of ICa,L. Forskolin (0.01 mumol/L) or isoproterenol (0.01 mumol/L) had no effect on basal ICa,L, but each accentuated the rebound increase in ICa,L. When adenylate cyclase was maximally stimulated with 1 mumol/L isoproterenol plus 2 mumol/L forskolin, ACh decreased ICa,L but failed to elicit rebound stimulation of ICa,L. Milrinone (10 mumol/L) increased basal ICa,L by 70 +/- 7% and significantly attenuated the rebound stimulation of ICa,L. Exposure to 1 mmol/L 8-bromo-cGMP elicited a small decrease in basal ICa,L, attenuated ACh-induced inhibition, and enhanced the rebound stimulation of ICa,L. Incubation in pertussis toxin prevented all ACh-induced changes in ICa,L. Inhibition of nitric oxide synthase by 100 mumol/L NG-monomethyl-L-arginine (L-NMMA) decreased basal ICa,L by -20 +/- 5%, prevented ACh-induced inhibition, and markedly attenuated the rebound stimulation of ICa,L. We conclude that in cat atrial myocytes ACh acts via M2 muscarinic receptors and pertussis toxin-sensitive G protein to inhibit basal ICa,L and that on withdrawal ACh elicits a rebound stimulation of ICa,L. Rebound stimulation of ICa,L is mediated via cAMP-dependent protein kinase A enhanced by ACh-induced inhibition of phosphodiesterase. (ABSTRACT TRUNCATED AT 400 WORDS)
Relationship of immunogenicity to protective potency in acellular pertussis vaccines

Comparison of the immunogenicity response and resistance to challenge in the modified intracerebral challenge assay induced by various acellular pertussis vaccines showed that these were not closely linked. The immunogenicity assay was effective for confirming the presence of specific antigenic components and was invaluable for detecting minor components present in co-purified vaccines. However, the magnitude of antibody responses was not consistently related to antigen concentration nor did it correlate with protection in the modified intracerebral challenge assay. The immunogenicity assay detected degradation of pertussis toxin and pertactin components but not of filamentous haemagglutinin or fimbriae 2 and 3 in denatured acellular pertussis vaccines. The modified intracerebral challenge assay was effective in detecting antigen degradation in all types of acellular pertussis vaccines including those of European/North American origin but was dominated by the response to pertussis toxin. Aerosol challenge was more sensitive in detecting denaturation of filamentous haemagglutinin or fimbriae. The modified intracerebral challenge assay was the only assay that provided a quantitative indication of protective activity. Both immunogenicity and challenge assays provided useful data on acellular pertussis vaccine properties but were complementary and not alternatives.
Scaling of in Vitro Membrane Permeability to Predict P-glycoprotein-Mediated Drug Absorption in Vivo

In a previous study, the concentration-dependent permeability of P-glycoprotein (P-gp) substrate drugs, quinidine, verapamil, and vinblastine, in several cell monolayers with different levels of P-gp expression was analyzed kinetically to obtain fundamental parameters for P-gp-mediated transport, Vmax and Km(app) values. Both Vmax and Km(app) values of each drug were found to show linear correlations with the expression level of P-gp. These findings imply the possibility of estimating the Vmax and Km(app) values of P-gp substrate drugs in the in vivo intestinal membrane on the basis of the P-gp expression level. In the present study, concentration-dependent drug permeability to the rat small intestines (upper jejunum and ileum) was simulated on the basis of Vmax and Km(app) values of each drug estimated from the P-gp expression level in the rat small intestines. To validate the predictability of these procedures, drug permeability in the rat small intestines was measured by the in situ single-pass perfusion method. It was confirmed that simulated permeability of each drug in the rat jejunum and ileum corresponded well with permeability measured by the in situ single-pass perfusion method. This study clearly demonstrated the potential to estimate the permeability of P-gp substrate drugs in the human intestine from its P-gp expression level and thus the possibility to predict the oral absorption of those drugs.
Clinical significance of hematologic parameters in non‐Hodgkin's lymphoma at diagnosis

Three hundred seventeen patients with non‐Hodgkin's lymphoma (NHL) (54 low grade, 180 intermediate grade, 76 high grade, and seven unclassified) treated with chemotherapy were evaluated for the presence of hematologic abnormalities at diagnostic staging. Anemia was present in 42%, leukopenia in 6%, thrombocytopenia in 13%, leukocytosis in 26%, and thrombocytosis in 14% at presentation. The presence of bone marrow involvement by lymphoma was more likely to be associated with leukopenia and thrombocytopenia than the absence of bone marrow involvement. Although anemia was slightly more common in patients with bone marrow lymphoma than in those without marrow lymphoma, the difference was not statistically significant. Hematologic parameters were similar for patients with B‐cell or T‐cell lymphoma. Evidence of bone marrow failure with multiple cytopenias was present in 26 patients (8%). Leukoerythroblastosis was present in 2%. Circulating lymphoma was present in 9.5%. Anemic patients had a shorter survival time than nonanemic patients, whether bone marrow was involved by lymphoma or not. Survival was not affected by the presence of leukopenia or mild leukocytosis, but, in patients without marrow lymphoma, leukocytosis with a leukocyte count greater than 20 × 109/I was associated with short survival length. Thrombocytopenia was associated with short survival time only in patients with bone marrow involvement by lymphoma. Patients with multiple cytopenias or leukoerythroblastosis had short survival times, but the presence of circulating lymphoma did not alter survival when compared with other patients with bone marrow involvement by lymphoma. These data suggest that hematologic evaluation at the time of diagnostic staging of NHL provides useful prognostic information that may have therapeutic implications.
An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing1[OPEN]

An organelle RNA recognition motif protein is required for psbF transcript editing and the production of efficient PSII in Arabidopsis. Loss-of-function mutations in ORGANELLE RNA RECOGNITION MOTIF PROTEIN6 (ORRM6) result in the near absence of RNA editing of psbF-C77 and the reduction in accD-C794 editing in Arabidopsis (Arabidopsis thaliana). The orrm6 mutants have decreased levels of photosystem II (PSII) proteins, especially PsbF, lower PSII activity, pale green pigmentation, smaller leaf and plant sizes, and retarded growth. Stable expression of ORRM6 rescues the orrm6 editing defects and mutant phenotype. Unlike ORRM1, the other known ORRM plastid editing factor, ORRM6, does not contain RNA editing interacting protein/multiple organellar RNA editing factor (RIP/MORF) boxes, which are required for ORRM1 to interact with site-specific pentatricopeptide repeat protein editing factors. ORRM6 interacts with RIP1/MORF8, RIP2/MORF2, and RIP9/MORF9, known components of RNA editosomes. While some plastid RRM proteins are involved in other forms of RNA processing and translation, the primary function of ORRM6 is evidently to mediate psbF-C77 editing, like the essential site-specific pentatricopeptide repeat protein LOW PSII ACCUMULATION66. Stable expression in the orrm6 mutants of a nucleus-encoded, plastid-targeted PsbF protein from a psbF gene carrying a T at nucleotide 77 significantly increases leaf and plant sizes, chlorophyll content, and PSII activity. These transformants demonstrate that plastid RNA editing can be bypassed through the expression of nucleus-encoded, edited forms of plastid genes.
Oil Price Dynamics and Business Cycles in Nigeria: A Bayesian Time Varying Analysis

The study investigated the dynamic relationship between oil prices and Real GDP growth in Nigeria over time by making use of time varying Bayesian VAR with Stochastic volatility. I distinguished between supply and demand shocks by means of a sign restriction. First, the study found that, the response of the Nigerian economy has been varying overtime. Also, GDP growth responds positively to both supply and demand shocks. However, the magnitude of the response to demand shock is larger compared to that of supply shocks. This suggests that overtime an increase in oil prices due to shocks in demand matter most for the Nigerian economy.
Comparison of Effects Between Central and Peripheral Stage I Lung Cancer Using Image-Guided Stereotactic Body Radiotherapy via Helical Tomotherapy

Lung cancer is a common malignant tumor with high morbidity and mortality. Here we compared the effects and outcome between central and peripheral stage I lung cancer using image-guided stereotactic body radiotherapy. From June 2011 to July 2013, a total of 33 patients with stage I lung cancer were enrolled. A total of 50 Gy in 10 fractions or 60 Gy in 10 fractions was delivered in the central arm (n = 18), while 50 Gy in 5 fractions in the peripheral arm (n = 15). Statistical analyses were performed using logistic regression analysis and Kaplan-Meier method. The mean follow-up time was 38.1 months. Three-month, 1-, 2-, and 3-year overall response rates were 66.7%, 83.3%, 61.1%, and 72.2% and 66.7%, 80%, 80%, and 80% in the central and peripheral arms, respectively. Three-year local control rates (94.4% vs 93.3%, P = .854), regional control rates (94.4% vs 86.7%, P = .412), and distant control rates (64.2% vs 61.7%, P = .509) had no differences between the central and the peripheral arms. Grade 2 radiation pneumonitis was observed in 6 of 18 patients in the central arm and in 1 of 15 patients in the peripheral arm (P = .92). Grade 2 radiation esophagitis was 5.7% in the central arm, while none occurred in the peripheral arm (P = .008). Five (15.1%) of all patients felt slight fatigue during radiotherapy. Other major complications were not observed. In conclusion, helical image-guided stereotactic body radiotherapy for central stage I lung cancer is safe and effective compared to peripheral stage I lung cancer.
Reg1p targets protein phosphatase 1 to dephosphorylate hexokinase II in Saccharomyces cerevisiae: characterizing the effects of a phosphatase subunit on the yeast proteome

Protein phosphatase 1 (Glc7p) and its binding protein Reg1p are essential for the regulation of glucose repression pathways in Saccharomyces cerevisiae. In order to identify physiological substrates for the Glc7p–Reg1p complex, we examined the effects of deletion of the REG1 gene on the yeast phosphoproteome. Analysis by two‐dimensional phosphoprotein mapping identified two distinct proteins that were greatly increased in phosphate content in reg1Δ mutants. Mixed peptide sequencing identified these proteins as hexokinase II (Hxk2p) and the E1α subunit of pyruvate dehydrogenase. Consistent with increased phosphorylation of Hxk2p in response to REG1 deletion, fractionation of yeast extracts by anion‐exchange chromatography identified Hxk2p phosphatase activity in wild‐type strains that was selectively lost in the reg1Δ mutant. The phosphorylation state of Hxk2p and Hxk2p phosphatase activity was restored to wild‐type levels in the reg1Δ mutant by expression of a LexA–Reg1p fusion protein. In contrast, expression of LexA–Reg1p containing mutations at phenylalanine in the putative PP‐1C‐binding site motif (K/R)(X)(I/V)XF was unable to rescue Hxk2p dephosphorylation in intact yeast or restore Hxk2p phosphatase activity. These results demonstrate that Reg1p targets PP‐1C to dephosphorylate Hxk2p in vivo and that the motif (K/R)(X) (I/V)XF is necessary for its PP‐1 targeting function.