Miracle-dz/UniFAR-FTUs huggingface dataset:
Optical imaging of neural activity in multiple auditory cortical fields of guinea pigs

Neural activity of multiple fields in the auditory cortex of anesthetized guinea pigs in response to pure tones was visualized by optical recording using a voltage-sensitive dye (RH795). Ten auditory fields were identified based on the tonotopic organization and response latency: the core fields consisting of the primary (AI) and secondary (AII) fields and surrounding belt areas consisting of dorso-anterior (DA), dorsal (D), dorso-posterior (DP), posterior (P), ventro-poster- ior (VP), ventro-medial (VM), ventro-anterior (VA) and ventral (V) fields. Tonotopic organization was observed in all the fields apart from DA, D, DP and V. Spatio-temporal displays suggest that the auditory information spreads from the core fields of AI and AII to belt fields via three distinct (dorsocaudal, caudal and ventrorostral) pathways.
Deep Neuromuscular Block Improves Surgical Conditions during Bariatric Surgery and Reduces Postoperative Pain: A Randomized Double Blind Controlled Trial

Background It remains unknown whether the administration of a deep neuromuscular block (NMB) during bariatric surgery improves surgical conditions and patient outcome. The authors studied the effect of deep versus moderate NMB in laparoscopic bariatric surgery on surgical conditions and postoperative pain. Methods and Results One hundred patients scheduled to undergo elective bariatric surgery were randomized to a deep NMB (post-tetanic-count 2–3) or a moderate NMB (train-of-four 1–2). The quality of the surgical field was scored using the Leiden-Surgical Rating Scale (L-SRS), a 5-point scale ranging from 1 (extremely poor conditions) to 5 (optimal conditions). Three surgeons scored the L-SRS at 10-min intervals during surgery; postoperative pain scores were obtained in the postanesthesia-care-unit (PACU) and on the ward. Mean (95% confidence interval) L-SRS scores in moderate NMB 4.2 (4.0–4.4) versus 4.8 (4.7–4.9) in deep NMB (p < 0.001). Moderate NMB resulted in 17% of scores at L-SRS scores of 1–3, while deep NMB resulted in 100% scores at the high end of the L-SRS (4–5). Deep NMB led to improved pain scores in the PACU (4.6 (4.2–4.9) versus 3.9 (3.6–4.4), p = 0.03) and reduced shoulder pain on the ward (1.8 (1.5–2.1) versus 1.3 (1.1–1.5), p = 0.03). A composite score of pain and opioid use in the PACU favoured deep NMB (p = 0.001). Conclusions In bariatric surgery, deep relaxation has advantages for surgeon and patient. Compared to moderate NMB, deep NMB produced stable and improved surgical conditions with less postoperative pain.
Mice lacking P2Y2 receptors have salt‐resistant hypertension and facilitated renal Na+ and water reabsorption

Extracellular nucleotides (e.g., ATP) regulate many physiological and pathophysiological pro‐cesses through activation of nucleotide (P2) receptors in the plasma membrane. Here we report that gene‐targeted (knockout) mice that lack P2Y2 receptors have salt‐resistant arterial hypertension in association with an inverse relationship between salt intake and heart rate, indicating intact baroreceptor function. Knockout mice have multiple alterations in their handling of salt and water: these include suppressed plasma renin and aldo‐sterone concentrations, lower renal expression of the aldosterone‐induced epithelial sodium channel α‐ENaC, greater medullary expression of the Na‐K‐2Cl‐cotrans‐porter NKCC2, and greater furosemide‐sensitive Na+ reabsorption in association with greater renal medullary expression of aquaporin‐2 and vasopressin‐depen‐dent renal cAMP formation and water reabsorption despite similar vasopressin levels compared with wild type. Of note, smaller increases in plasma aldosterone were required to adapt renal Na+ excretion to restricted intake in knockout mice, suggesting a facilitation in renal Na+ retention. The results thus identify a previously unrecognized role for P2Y2 receptors in blood pressure regulation that is linked to an inhibitory influence on renal Na+ and water reabsorption. Based on these findings in knockout mice, we propose that a blunting in P2Y2 receptor expression or activity is a new mechanism for salt‐resistant arterial hypertension. —Rieg, T. , Bundey, R. A. , Chen, Y. , Deschenes, G. , Junger, W. , Insel, P. A. , Vallon, V. Mice lacking P2Y2 receptors have salt‐resistant hypertension and facilitated renal Na+ and water reabsorption FASEB J. 21, 3717–3726 (2007)
Trends in drug-resistant tuberculosis in the United States, 1993-1996.

CONTEXT With the resurgence of tuberculosis (TB) disease in the late 1980s and early 1990s in the United States, multidrug-resistant (MDR) TB emerged as a serious challenge to TB control. In response, the Centers for Disease Control and Prevention in 1993 added drug susceptibility test results to the information collected for the national surveillance system to monitor trends in drug resistance. OBJECTIVE To determine the extent of drug-resistant tuberculosis (TB) in the United States. DESIGN Descriptive analysis of TB surveillance data. STUDY POPULATION Patients reported to the national TB surveillance system as confirmed TB cases with culture-positive disease from 1993 through 1996 by the 50 states, New York City, and the District of Columbia (DC). MAIN OUTCOME MEASURE Percentage of case patients with culture-positive disease whose isolates are resistant to specific anti-TB drugs. RESULTS Overall resistance to at least isoniazid was 8.4%; rifampin, 3.0%; both isoniazid and rifampin (ie, MDR TB), 2.2%; pyrazinamide, 3.0%; streptomycin, 6.2%; and ethambutol hydrochloride, 2.2%. Rates of resistance were significantly higher for case patients with a prior TB episode. Among those without prior TB, isoniazid resistance of 4% or more was found in 41 states, New York City, and DC. A total of 1457 MDR TB cases were reported from 42 states, New York City, and DC; however, 38% were reported from New York City. Rates of isoniazid and streptomycin resistance were higher for cases among foreign-born compared with US-born patients [corrected] but rates of rifampin resistance and MDR TB were similar. Among US-born patients, resistance to first-line drugs, particularly rifampin monoresistance, was significantly higher among those with human immunodeficiency virus (HIV) infection. CONCLUSIONS Compared with recent US surveys in 1991 and 1992, isoniazid resistance has remained relatively stable. In addition, the percentage of MDR TB has decreased, although the national trend was significantly influenced by the marked decrease in New York City. Foreign-born and HIV-positive patients and those with prior TB have higher rates of resistance. The widespread extent of isoniazid resistance confirms the need for drug susceptibility testing to guide optimal treatment of patients with culture-positive disease.
Sport disciplines, types of sports, and waist circumference in young adulthood – a population-based twin study

Abstract Purpose: The benefits of physical activity (PA) in preventing abdominal obesity are well recognized, but the role of different sport disciplines remains open. We aimed, therefore, to investigate how participation in different sport disciplines, and the number and types of sports engaged in are associated with waist circumference (WC) in young adulthood. Methods: This population-based cohort study comprised 4027 Finnish twin individuals (1874 men), with a mean age of 34 y (32–37), who answered a survey, including self-measured WC. We extracted the number and identified the types (aerobic, power, and mixed) of the different sport disciplines respondents reported participating in. Results: The number of sport disciplines participated in was inversely associated with WC, the linear decrease averaging 1.38 cm (95% CI 1.10–1.65) per each additional sport discipline. The result persisted after adjustment for the main covariates, such as volume of PA and diet quality. Among dizygotic twin pairs discordant for sports participation (0–2 vs. 5 or more disciplines), the mean within-pair difference in WC was 4.8 cm (95% CI 0.4–9.1) for men and 11.2 cm (95% CI 4.4–18.0) for women; among discordant monozygotic pairs, no differences were observed. In men, all three types of sports were individually associated with smaller WC, while in women, only mixed and power sports showed this association. Conclusions: Participation in several sport disciplines and sport types was associated with smaller WC among young adults in their mid-30s. Shared genetic background may explain some of the associations.
SUPPRESSION OF EXTRAINTESTINAL AND INTESTINAL NIPPOSTRONGYLUS BRASILIENSIS-INDUCED EOSINOPHILIA BY EIMERIA NIESCHULZI

Eosinophil responses in extraintestinal and intestinal tissues were examined in August and Sprague-Dawley rats infected with Nippostrongylus brasiliensis or Eimeria nieschulzi (or both), and in uninfected controls to test the hypothesis that E. nieschulzi suppresses the systemic N. brasiliensis-induced eosinophil response. Caudal vein blood, femoral bone marrow, bronchoalveolar lavage fluid, peritoneal lavage fluid, and duodenal and jejunal samples were collected on day 8 postinfection (PI) with E. nieschulzi, on day 16 PI of the N. brasiliensis infection, when these days coincided in the concurrently infected rats, and from uninfected controls. Differential white blood cell counts were made from blood smears and cytocentrifuged preparations, and duodenal and jejunal eosinophils per villus crypt unit were quantified. Eimeria nieschulzi significantly reduced N. brasiliensis-induced eosinophil levels in peripheral blood, lavage fluids, and duodenal and jejunal tissues in both rat strains. August and Sprague-Dawley rats monospecifically infected with N. brasiliensis and concurrently with both parasites demonstrated elevated eosinopoiesis compared with uninfected controls and rats infected with only E. nieschulzi; however, despite this, concurrently infected rats had a significantly greater level of eosinopoiesis than those infected with only the nematode. In addition, E. nieschulzi induced elevated neutrophil levels in both monospecifically and concurrently infected rats in all extraintestinal tissues examined in both rat strains, whereas lymphocyte counts decreased concomitantly. This study suggests that the intestinal coccidian E. nieschulzi has the ability to modulate the systemic inflammatory response to N. brasiliensis and that this is not a rat strain-specific phenomenon.
Necessary Elements for Effective Worker Participation in Decision Making

For many decades, conventional managerial practice relied on strict hierarchical and authoritarian relationships to direct and coordinate a smooth flow of production. Managers' personal predilections for strict control were supported by Weberian views of the "rationalized" administrative system, which held that the most efficient performance of subordinates would be obtained by increasing the organization's degree of standardization, impersonality, specialization, routine, formal regulations, and promotion through levels of hierarchy to create a career [Weber 1947, pp. 330-40]. Beneath this administrative apparatus, the tenets of "scientific management" were applied to the production line. This approach assumed that the greatest efficiency would be obtained by fragmenting the overall production assignment into smaller and smaller operations, each of which is to be performed by an individual repeating a simple task,
The MPTP-treated primate as a model of motor complications in PD

Motor complications are a common cause of treatment failure in Parkinson’s disease (PD). Although the underlying mechanisms remain obscure, research with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated primates may facilitate their investigation. Repeated administration of l-dopa to MPTP-treated primates results in a loss of drug efficacy (“wearing off”) and periods of immobility (“on-off”). Immediately after each dose of l-dopa, animals show an initial worsening of motor performance (“beginning-of-dose deterioration”) and, at the end of action of each dose, they exhibit a decline to levels of disability below those seen at baseline (“rebound worsening”). Dyskinesia (chorea, dystonia, athetosis) appears rapidly in MPTP-treated primates given l-dopa. The greater the degree of nigral denervation, the less exposure to l-dopa that is required to induce dyskinesia. The onset and intensity of dyskinesia are related to the dose and frequency of l-dopa administration. Increasing brain exposure to l-dopa increases the severity and intensity of involuntary movements. In contrast, long-acting dopamine agonists (DAs) induce a far lower incidence of dyskinesia, and continuous dopaminergic stimulation may provide a means of avoiding its onset. Recent data from MPTP-treated primates suggest that switching from l-dopa to a long-acting DA may reverse the priming process associated with induction of dyskinesia. Responses of MPTP-treated primates have proved to be highly predictive of antiparkinsonian activities of drugs in humans and their ability to induce dyskinesia, but they may also have utility in evaluating the mechanisms that underlie a range of long-term motor complications affecting patients with PD.
Growth Effects of Remittances in Bangladesh: Is there a U‐shaped Relationship?

This article shows that the effect of remittances on economic growth involves a U-shaped pattern, which is negative initially but later becomes positive. The analysis differs significantly from earlier studies in that it examines important methodological issues on the specification and estimation of the long-run growth effects of remittances by estimating their impact on total factor productivity (TFP) rather than on the growth rate of GDP, using time series data from Bangladesh. The use of single-equation cointegration methods shows that remittances’ effect on long-run growth in Bangladesh is negative and falling until the remittances-to-GDP ratio is roughly eight per cent. The benefits of remittances receipts outweigh their costs and their net effects start to become positive when the ratio exceeds 14 per cent.
A Transitions-Based Model of Default for Irish Mortgages

Using a uniquely constructed loan-level dataset of the residential mortgage book of Irish financial institutions, this paper provides a framework for estimating default probabilities of individual mortgages. In contrast to the popular stock delinquency approach, this model provides estimates of default and cure flows: a requirement of the stress test approach adopted by the European Central Bank's comprehensive assessment. In addition, both default and cure transitions are modelled as functions of micro- and macro-covariates including loan characteristics and current macroeconomic conditions such as house prices and unemployment. When comparing the competing equity and affordability effects, labour market deterioration played a stronger role than house equity in the rise of Irish default rates. For cures, a scarring effect of default is identified and estimated with the probability of a loan returning to performing reducing by 25 per cent each quarter a loan remains delinquent.
Antioxidant Activity and Total Phenolic Content of Some Brazilian Species

Abstract The antioxidant activity of extracts and fractions of six vegetal species from the Brazilian Atlantic Forest were determined. The total antioxidant activity was assessed based on the scavenging activity of the stable DPPH free radical. Eight extracts or fractions of plants showed significant DPPH scavenging activity (IC50 ≤ 10.0 µg/mL) compared with the values obtained for ascorbic acid (IC50 = 8.4 µg/mL) and gallic acid (IC50 = 2.6 µg/mL). The extracts or fractions were as follows: ethanol extracts of leaves, flowers, and stems of Baccharis illinita. DC. , ethanol extracts of leaves and stems of B. platypoda. DC. , hydroalcoholic extract and ethyl acetate fraction of leaves of Cyathea phalerata. Mart. and hydroalcoholic extract of bark of Trichilia catigua. A. Juss. Seven flavonoids present in the plant extracts were also investigated. The most active compounds were taxifolin, quercetin, and luteolin, which possess the catechol group 3′,4′-diOH. In addition, the total phenolic or flavonoid contents of these extracts and fractions were evaluated. The phenolic content of the sample was determined using Folin-Ciocalteu reagent and varied from 489.07 to 11.29 mg/g dry weight expressed as gallic acid equivalents (GAE). The total flavonoid concentrations, detected using 2% aluminum chloride, varied from 61.82 to 5.6 mg quercetin equivalents (QE)/g dry weight. These results suggest that the level of antioxidant activity in these plants varies by a great extent. They also suggest that the phenolic content in these plants provides substantial antioxidant activity. The flora of Brazil appears to be a rich and interesting source for supplementary ethnomedical and phytochemical studies.
Conformational Nature of the Borrelia burgdorferiDecorin Binding Protein A Epitopes That Elicit Protective Antibodies

ABSTRACT Decorin binding protein A (DbpA) has been shown by several laboratories to be a protective antigen for the prevention of experimental Borrelia burgdorferi infection in the mouse model of Lyme borreliosis. However, different recombinant forms of the antigen having either lipidated amino termini, approximating the natural secretion and posttranslational processing, or nonprocessed cytosolic forms have elicited disparate levels of protection in the mouse model. We have now used the unique functional properties of this molecule to investigate the structural requirements needed to elicit a protective immune response. Genetic and physicochemical alterations to DbpA showed that the ability to bind to the ligand decorin is indicative of a potent immunogen but is not conclusive. By mutating the two carboxy-terminal nonconserved cysteines of DbpA from B. burgdorferi strain N40, we have determined that the stability afforded by the putative disulfide bond is essential for the generation of protective antibodies. This mutated protein was more sensitive to thermal denaturation and proteolysis, suggesting that it is in a less ordered state. Immunization with DbpA that was thermally denatured and functionally inactivated stimulated an immune response that was not protective and lacked bactericidal antibodies. Antibodies against conformationally altered forms of DbpA also failed to kill heterologous B. garinii and B. afzelii strains. Additionally, nonsecreted recombinant forms of DbpAN40were found to be inferior to secreted lipoprotein DbpAN40 in terms of functional activity and antigenic potency. These data suggest that elicitation of a bactericidal and protective immune response to DbpA requires a properly folded conformation for the production of functional antibodies.
Corrected QT Interval Changes During Electroconvulsive Therapy-Antidepressants-Atypical Antipsychotics Coadministration: Safety Issues

Both old and newer antidepressants and some atypical antipsychotics, such as ziprasidone and quetiapine, used as augmentation agents in severe major depression, are known to increase corrected QT (QTc) interval. We investigated the safety of the coadministration of electroconvulsive therapy with an antidepressant-ziprasidone-quetiapine combination with respect to QTc interval in 6 female patients with severe major depression resistant to pharmacotherapy alone. Each patient underwent a series of 10 to 11 sessions of bilateral electroconvulsive therapy. Corrected QT intervals were calculated at baseline and several times up to 10 minutes after seizure cessation. Overall, QTc interval changes remained within normal limits, without the occurrence of any cardiac adverse events. Our findings suggest that the coadministration of these treatments might be safe, at least with respect to QTc interval changes.
Meta-analysis of adverse events in recent randomized clinical trials for dimethyl fumarate, glatiramer acetate and teriflunomide for the treatment of relapsing forms of multiple sclerosis

Purpose/Aim of the Study: Trials of dimethyl fumarate (DMF) and teriflunomide, two new oral therapies for relapsing-remitting multiple sclerosis (RRMS) were recently published [1, 2, 3]. A comparison of their safety against glatiramer acetate–a prevalent injectable treatment–is relevant to inform therapy-switching decisions. The study objective was to conduct a systematic review and mixed treatment comparison of total AEs in RCTs of dimethyl fumarate 240 mg bid (DMF2) or tid (DMF3), glatiramer acetate 20 mg injectable daily (GA), and teriflunomide 7 mg (TERI7) or 14 mg (TERI14) daily in RRMS patients. Materials and Methods: Articles were selected following Cochrane guidelines. A network meta-analysis was used to compare the odds of patients experiencing at least one AE between drugs, using placebo as baseline. Drugs were compared using the odds ratio (OR), credible interval (CrI), and confidence in OR ≥1 (PrOR). The mean rank (best = 1) and corresponding Surface-Under-Cumulative-Ranking (SUCRA) (best = 100%) were reported. Results: 3737 patients from three RCTs were included for analysis. Patients receiving GA exhibited the lowest AEs (DMF2 [OR = 2.67, PrOR = 98.7%], DMF3 [OR = 1.92, PrOR = 95.3%], Teri7 [OR = 2.74, PrOR = 95.2%], Teri14 [OR = 3.03, PrOR = 96.4%]), and equivalent to PB (OR = 1.60; PrOR = 94.3%). No other significant differences were found. GA also ranked with the lowest AEs (rank = 1.2, SUCRA = 96.0%), whereas DMF2 and Teri14 ranked highest (rank = 4.8). Conclusions: RRMS patients treated with glatiramer have the lowest odds of experiencing AEs, while patients taking DMF or teriflunomide have similar, higher odds of developing AEs, suggesting that patients treated with glatiramer may have higher QoL than patients under DMF or teriflunomide.
Effect of follicular fluid treatment on follicle-stimulating hormone, luteinizing hormone and compensatory ovarian hypertrophy in prepuberal gilts.

Prepuberal 130-day-old gilts were treated with 10 ml of charcoal-stripped porcine serum (PS), whole porcine follicular fluid (WpFF) or charcoal-stripped pFF (CpFF) twice daily beginning the day before and continuing 8 days after unilateral ovariectomy (ULO). Follicle-stimulating hormone (FSH) declined for the first 14 h after ULO in WpFF and CpFF gilts and then by 24 h returned to values observed at or before ULO, whereas FSH was increased nearly twofold at 14 h in PS gilts. At 8 days after ULO the remaining ovaries from PS-treated gilts were heavier than ovaries from follicular fluid-treated gilts. In a second experiment, ovariectomized 130-day-old gilts were assigned to either a group infused with PS, a group infused with 5 ml CpFF, or a group infused with 10 ml Cpff at 18 and 2 h before a gonadotropin-releasing hormone (GnRH) challenge. Porcine follicular fluid had no effect on luteinizing hormone (LH) response to GnRH, depressed the FSH response to a 10-micrograms challenge of GnRH, but had no effect on FSH response to a 50-micrograms challenge of GnRH. In a third study, gilts were subjected to sham ovariectomy (Sham) or ULO at 130 days of age. GnRH (10 micrograms) was given on Days 1, 2 or 8 after surgery. The response to GnRH in ULO versus Sham gilts did not differ for FSH or LH on any day. (ABSTRACT TRUNCATED AT 250 WORDS)