Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence of Temperature on Cryptosporidium parvum Oocyst Infectivity in River Water Samples as Detected by Tissue Culture Assay

Cryptosporidium parvum oocysts were stored in 1-ml aliquots of filtered river water at −20, 4, 10, and 21–23 C in the dark. Oocysts were also added to filter-sterilized river water samples and stored at 21–23 C. The infectivity of oocysts stored under different conditions was assayed at weekly intervals through infection of human adenocarcinoma ileocecal (HCT-8) cell monolayers. Wells containing between 10 and 100 foci of infection were enumerated by immunofluorescent microscopy, and the number of infective oocysts was calculated. No infectious oocysts were detected after 1 wk at −20 C. The number of infective oocysts stored at 4 C decreased 5-fold, and the number of those stored at 10 C decreased 2.5-fold after 14 wk. The infectivity of oocysts stored in potassium dichromate (positive control) at 4 C decreased 2-fold over 14 wk. The number of infective oocysts in filter-sterilized and non–filter-sterilized river water stored at 21–23 C decreased by 3.3 and 2.6 log units, respectively, over 12 wk, and no foci of infection were detected at 14 wk. The results show that as temperature increased from 4 to 23 C, the duration of oocyst infectivity decreased.
Therapeutic applications of hydrogels in oral drug delivery

Introduction: Oral delivery of therapeutics, particularly protein-based pharmaceutics, is of great interest for safe and controlled drug delivery for patients. Hydrogels offer excellent potential as oral therapeutic systems due to inherent biocompatibility, diversity of both natural and synthetic material options and tunable properties. In particular, stimuli-responsive hydrogels exploit physiological changes along the intestinal tract to achieve site-specific, controlled release of protein, peptide and chemotherapeutic molecules for both local and systemic treatment applications. Areas covered: This review provides a wide perspective on the therapeutic use of hydrogels in oral delivery systems. General features and advantages of hydrogels are addressed, with more considerable focus on stimuli-responsive systems that respond to pH or enzymatic changes in the gastrointestinal environment to achieve controlled drug release. Specific examples of therapeutics are given. Last, in vitro and in vivo methods to evaluate hydrogel performance are discussed. Expert opinion: Hydrogels are excellent candidates for oral drug delivery, due to the number of adaptable parameters that enable controlled delivery of diverse therapeutic molecules. However, further work is required to more accurately simulate physiological conditions and enhance performance, which is important to achieve improved bioavailability and increase commercial interest.
Do normal head ultrasounds need repeating in infants less than 30 weeks gestation?

Abstract Objective: Current head ultrasound (HUS) screening recommendations in preterm infants often include a repeat HUS, regardless of initial findings. The objective of this study is to determine the rate of subsequent severe intraventricular hemorrhage (IVH), ventriculomegaly (VM), or periventricular leukomalacia (PVL) among infants < 30 weeks gestation (EGA) with a normal HUS at day of life (DOL) 4–10. Methods: Retrospectively collected data were analyzed for all infants < 30 weeks EGA cared for in one NICU from 1 January 2010 to 31 August 2014. Infants with severe congenital anomalies were excluded. We reviewed the first three HUSs and last documented HUS. Severe IVH was defined as > Papile grade 2 and significant interval HUS change was defined as development of severe IVH, PVL, or VM. Results: Of the 383 infants who had an initial screening HUS between DOL 4 and 10, 258 (67%) were initially normal and repeat screening was performed in 228 of these. None developed severe IVH on follow-up HUS. One infant developed VM secondary to GBS meningitis, and one developed echogenicity concerning for PVL that later resolved. Conclusions: Among very preterm infants with a normal HUS between DOL 4 and 10, routine follow-up HUS is unlikely to identify a significant change.
Vaccine preventable diseases and vaccination coverage in Australia, 1993-1998.

BACKGROUND Since the introduction of childhood vaccination for diphtheria in 1932 and the widespread use of vaccines to prevent tetanus, pertussis (whooping cough) and poliomyelitis in the 1950s, deaths in Australia from vaccine preventable diseases (VPDs) have declined by more than 99%. It is important, however, that the downward trend in morbidity and mortality from VPDs is maintained and carefully monitored, and that changes are interpreted in relation to vaccination coverage. AIM This report aimed to bring together three national sources of routinely collected data on the morbidity and mortality (notifications, hospitalisations and deaths) from VPDs during the period 1993-1998 for the 8 diseases then on the routine childhood vaccination schedule, and for 4 other diseases potentially preventable by childhood vaccination. It also examined vaccination coverage for the same period. METHODS Data sources included notifications from the National Notifiable Diseases Surveillance System (NNDSS), hospitalisation data from the Australian Institute of Health and Welfare (AIHW) National Hospital Morbidity Database, deaths from the Australian Bureau of Statistics (ABS) Causes of Death Collection and vaccination coverage according to the Australian Childhood Immunisation Register (ACIR). All data sources were expected to have some limitations, the most important being under-reporting for notifications and vaccination encounters, and coding errors in the hospital morbidity data. RESULTS Notifications for the 8 diseases covered by the routine schedule declined by 42%, from an average of 11,537 cases each year in 1993-1997 to 6700 in 1998, and hospitalisations fell by 12%, from an average of 1745 per year to 1536 in 1997/1998, while deaths remained unchanged at 7 each year over the period of review (Table 1). Tetanus caused 1 or 2 of the deaths each year. However, 6 of the 7 deaths in 1997 were in infants during a major outbreak of pertussis. Pertussis caused most of the notifications, hospitalisations and deaths during the review period. While most of these were in children, 46% of the notifications and 13% of the hospitalisations occurred in persons aged 15 years or more. There were notable declines in the numbers of notifications of invasive Haemophilus influenzae type b (Hib) disease in children under 5 years of age (77%), measles (87%) and rubella (75%), and there were no notifications of diphtheria or poliomyelitis. Vaccination coverage estimated using ACIR data increased during the review period. Coverage for the first 3 doses of diphtheria, tetanus, pertussis and Hib vaccines, assessed at 1 year of age, increased from 75% to 85%, while coverage for measles-mumps-rubella (MMR) vaccine, assessed at 2 years of age, increased from 83% to 86%. It is likely that these data underestimated coverage by 5-10%, and that the increase in coverage partly reflected better reporting to the ACIR by providers.
DSM-IV versus DSM-5: implementation of proposed DSM-5 criteria in a large naturalistic database.

OBJECTIVE Problems with the current DSM-IV eating disorder (ED) section have resulted in proposed changes toward the upcoming DSM-5 (http://www.dsm5.org/ProposedRevisions/Pages/EatingDisorders.aspx). We investigated consequences of these by implementing the proposal in a large naturalistic database. METHOD Patients were 2,584 children/adolescents and adults enrolled at specialized ED clinics in Sweden. DSM-IV diagnoses anorexia nervosa, bulimia nervosa, and "not otherwise specified" examples were compared with DSM-5 anorexia, bulimia, and binge ED, as well as atypical anorexia, subthreshold bulimia, and binge eating, purging disorder, and the residual unspecified category. Assessment methods included a semistructured diagnostic interview and self-ratings of ED and psychiatric symptoms. RESULTS We studied age-separated diagnostic distributions and explained variance in clinical variables associated with the two systems. Results showed some improvement of diagnostic specification as well as a slight increase in explained variance. DISCUSSION Remaining problems with the proposed changes were also highlighted, and possible further refinement is discussed.
Comparison of Memory and Combined Exercise and Memory-Anchoring Procedures on Ratings of Perceived Exertion during Short Duration, Near-Peak-Intensity Cycle Ergometer Exercise

The purpose of this study was to compare ratings of perceived exertion (RPE) following memory-anchoring and two different types of combined exercise and memory-anchoring during short duration, near-peak-intensity cycle exercise. Thirty recreationally trained males volunteered to participate. The M group, n=10, received only verbal instructions prior to the experimental trial. The EM1 group, n = 10, and the EM2 group, n = 10, received the same verbal instructions, but these were administered while participants performed maximal, graded cycle ergometer exercise. The low perceptual anchor was established during light pedaling for both EM1 and EM2. The high perceptual anchor was established during the final stage of the maximal cycle test for EM1 and during a 30-sec. sprint immediately following the final stage of the maximal cycle ergometer testing for EM2. On the experimental trial pedaling at maximal intensity for 30-sec. was against a resistance equal to . 10 × body mass (kg) on a cycle ergometer. The Borg 15-category RPE scale was used to record exertional perceptions. RPE was reported at 8, 13, 18, 23, and 28 sec. each trial. Ratings were similar among the three groups. Their linear regression slopes and intercepts were also similar. Memory-anchoring produced similar RPE for two different combined exercise and memory-anchoring procedures. In conclusion, memory-anchoring and combined exercise and memory-anchoring produce similar RPE during high intensity, short duration cycle exercise in young recreationally trained athletes.
Health-related quality of life in individuals with chronic ankle instability.

CONTEXT Individuals with chronic ankle instability (CAI) have reported decreased global and regional function. Despite the identification of functional deficits in those with CAI, more research is required to determine the extent to which CAI influences the multidimensional profile of health-related quality of life. OBJECTIVE To determine whether global, regional, and psychological health-related outcomes differ between individuals with and without CAI. DESIGN Case-control study. SETTING Laboratory. PATIENTS OR OTHER PARTICIPANTS Twenty-five participants with CAI (age = 21.9 ± 2.5 years, height = 170.8 ± 8.6 cm, mass = 69.8.0 ± 11.7 kg) were sex- and limb-matched to 25 healthy participants (age = 22.0 ± 2.1 years, height = 167.4 ± 9.1 cm, mass = 64.8 ± 11.2 kg). MAIN OUTCOME MEASURE(S) Both groups completed the Disablement in the Physically Active Scale, the Foot and Ankle Ability Measure (FAAM), the FAAM-Sport, the Tampa Scale of Kinesiophobia-11, and the Fear-Avoidance Beliefs Questionnaire. Dependent variables were scores on these instruments, and the independent variable was group. RESULTS Compared with healthy individuals, those with CAI reported decreased function on the Disablement in the Physically Active Scale, FAAM, and FAAM-Sport (P < .001) and increased fear of reinjury on the Tampa Scale of Kinesiophobia-11 and Fear-Avoidance Beliefs Questionnaire (P < .001). In the CAI group, the FAAM and FAAM-Sport demonstrated a significant relationship (r = 0.774, P < .01). CONCLUSIONS Individuals with CAI reported decreased function and increased fear of reinjury compared with healthy control participants. Also, within the CAI group, there was a strong relationship between FAAM and FAAM-Sport scores but not between any other instruments. These findings suggest that health-related quality of life should be examined during the rehabilitation process of individuals with CAI.
Essays in Corporate Finance

This dissertation studies the effects of informational asymmetries on corporate finance. Two distinct settings are considered, one empirical and one theoretical. In the first chapter, I show that strong creditor rights facilitate the financing of innovation, using a novel dataset of patent collateral portfolios. Using the random timing of court decisions as a source of exogenous variation in creditor rights, I show that patenting companies raise more debt financing when they can more credibly pledge their patents as collateral. Consequently, R&D investment and patenting output also increase, as do the technological diversity and average citation count of the patents produced. These findings demonstrate that low leverage in innovative industries is due to information asymmetry, not incentive problems, and that a strengthening of creditor rights alleviates this friction by increasing collateral values. In the second chapter, we identify three new factors that affect the firm's decision between equity issuance and asset sales in the presence of information asymmetry. First, equity investors own a claim to the capital raised, which mitigates the information asymmetry of the underlying balance sheet (the "certainty effect"). Second, firms can disguise the sale of a low-quality asset as instead motivated by dissynergies, and thus receive a higher price (the "camouflage effect"). Third, unlike with an equity issuance, a "lemons" discount on assets need not lead to a low stock price, as the asset is not a carbon copy of the firm (the "correlation effect"). The findings demonstrate that information asymmetry is a function of the nature and purpose of the firm's financing decisions. Degree Type Dissertation Degree Name Doctor of Philosophy (PhD) Graduate Group Finance First Advisor Michael R. Roberts Subject Categories Finance and Financial Management This dissertation is available at ScholarlyCommons: http://repository.upenn. edu/edissertations/1358 ESSAYS IN CORPORATE FINANCE William Mann A DISSERTATION in Finance For the Graduate Group in Managerial Science and Applied Economics Presented to the Faculties of the University of Pennsylvania in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy 2014 Supervisor of Dissertation Michael R. Roberts William H. Lawrence Professor of Finance Graduate Group Chairperson Eric Bradlow K.P. Chao Professor, Marketing, Statistics, and Education Dissertation Committee Michael R. Roberts, William H. Lawrence Professor of Finance Todd Gormley, Assistant Professor of Finance Mark Jenkins, Assistant Professor of Finance Alex Edmans, Professor of Finance, London Business School
The Power of a Single Number: A Political History of GDP

GDP has been relied upon at every turn by the coalition government to demonstrate the credibility of its ‘long­term economic plan’. Ahead of the general election, LSE alumnus Philipp Lepenies will question how GDP came to be indispensable as the indicator for progress, and demonstrate why any attempt to reduce the impact of this figure on policy needs to start by understanding how it came to occupy the throne in the first place.
Nuclear localization of β-catenin in adult mouse thalamus correlates with low levels of GSK-3β

Besides its well established role in development and tumorogenesis, nuclear translocation of beta-catenin has also been suggested to play a role in adult brain physiology and pathology. However, nuclear localization of beta-catenin has never been observed in adult brain tissue. Immunohistochemical analysis of beta-catenin distribution in the adult mouse brain revealed nuclear localization exclusively in the whole thalamus with the exception of the reticular nucleus. To investigate whether differences in the level of beta-catenin or GSK-3beta (the enzyme that targets it for degradation by the proteasome) might account for the differential localization in thalamus we performed Western analysis of various brain tissues. The beta-catenin/GSK-3beta ratio was higher in thalamus than in the rest of the brain, suggesting a key role of GSK-3beta in this phenomenon.
The Impact of Education on Political Ideology: Evidence from European Compulsory Education Reforms

Previous research documents a correlation between education and political ideology, usually indicating a positive relationship between education and left-wing political views. In this paper, I examine to what extent this association is causal. I merge political ideology data from 25 waves of Eurobarometer surveys with information on 18 educational reforms in 11 European countries. I then instrument for educational attainment with a regression discontinuity design that estimates the increase in education due to compulsory educational reforms. Notably, it appears that omitted variables bias is important here. I find a significant causal effect of education moving individuals to the right when properly addressing the endogeneity whereas there is a significant association between education and left-wing political ideology when treating education as exogenous. I find that on average, among the individuals compelled into additional education from these specific reforms, an additional year of education moves individuals to the right of the political continuum by about 5–6%. However, I also find no evidence of a causal effect on political ideology for a subgroup of countries.
Murine respiratory mycoplasmosis in F344 and LEW rats: evolution of lesions and lung lymphoid cell populations

By comparison of two strains, LEW and F344, which are known to differ in susceptibility to Mycoplasma pulmonis respiratory disease, it was shown that differences in lesion severity and progression were associated with changes in lung lymphocyte populations. Lung lesions in LEW rats developed earlier after infection, became more severe, and were characterized by continued proliferation of all classes of lymphoid cells, T lymphocytes, B lymphocytes, and plasma cells, throughout the 120-day observation period. In contrast, lymphoid proliferation in F344 rats reached a plateau at 28 days and was restricted to an increase in T lymphocytes, immunoglobulin A (IgA)-bearing B lymphocytes, and IgA and IgG plasma cells. Although approximately 10 times as many IgG B cells and 4 times as many IgG plasma cells were found in infected LEW rats as compared with F344 rats, the specific anti-M. pulmonis IgG response in the two strains was roughly parallel. The same relationships held true, although to a lesser extent, for specific IgA antibody responses and cellular responses. Whereas lung lesions showed a tendency to resolve in F344 rats by 120 days, severe lesions persisted in LEW rats. The disparity between the cellular response and specific antibody response, the seemingly uncontrolled lymphocyte proliferation in LEW rats, and the mitogenic potential of M. pulmonis suggest that differences between LEW and F344 rats in lung lesion severity and progression are related to differences in the degree of nonspecific lymphocyte activation in the two strains, an imbalance in regulation of lymphocyte proliferation in LEW rats, or both.
Fusions of flagellar operons to lactose genes on a mu lac bacteriophage

Previous studies have defined 29 genes necessary for synthesis of the Escherichia coli flagellar apparatus. This study analyzed the transcriptional control of flagellar genes, using Mu d (Apr lac) phage to generate flagellar mutants by insertion. These mutants contained operon fusions of flagellar genes to the lac genes of the Mu d phage and allowed the measurement of flagellar operon expression by detection of beta-galactosidase activity. These fusion mutants expressed the enzyme activity constitutively, and an autogenous regulation mechanism was not revealed. Lambda transducing phages carrying these chromosomal fla-lac fusions were also isolated and used to examine the effect of different fla mutations on expression of each flagellar operon. The results showed that flagellar operons are divided into six classes; (class 1) the flbB operon, which controls all of the other flagellar operons; (class 2) the flaU and flbC operons, which are controlled by the flbB operon gene products and are not required for the expression of other Fla operons; (class 3) the flbA, flaG, flaD, flaN, flaB, and flaA operons, which are under flbB operon control and are required for the expression of other fla operons; (class4) the flaZ operon, which is controlled by the gene products of the group 1 and 3 operons and is required for hag transcription; (class 5) the mocha and flaS operons, which are controlled by the gene products of the group 1 and 3 operons; and (class 6) the hag operon. These results are discussed with respect to the possible assembly sequence of the fla gene products.
Revisiting estimation methods of business zakat and related tax incentives

Purpose Available zakat accounting standards as well as the laws governing business zakat suggest that the adjusted net working capital or the adjusted growth capital of a business may be regarded as the base for computation of its zakat liability. The apparent consensus follows from the fiqhi prescription of imposing zakat on urud al-tijarah or the inventory of goods available for trade. Some contemporary scholars however question the rationale underlying this method and argue that the objectives of the Shariah are better met by seeking recourse to alternative methods of zakat determination for business organizations. There is therefore a need to revisit the issues and subject them to fresh scrutiny in terms of economic rationality and consistency. Design/methodology/approach The paper examines the arguments of the “orthodox” school as well as those of some contemporary scholars on alternative methods of computing business zakat. It also undertakes a comprehensive review of the laws of zakat as they are related to businesses and the related accounting pronouncements along with their underlying rationale. As the issue of incentivizing zakat payment is an important one, and it is often linked to provision of tax benefits, the paper examines a few suggestions in this regard. Findings On examination of specific suggestions – specifically, of treating earnings as zakat base – to scrutiny in terms of economic rationality and consistency, the authors argue that the “orthodox” position is not only consistent but also makes enormous economic sense. Further, the issue of incentivizing zakat payment and that of lack of harmonization between business zakat accounting and taxation need not be and should not be resolved by making changes in the former because the same has a sound Shariah basis. It can be easily resolved, if need be, by making changes in methods of taxation (tax deduction or tax rebate) and base them on specific items in the balance sheet or the income statement. Practical implications The paper provides useful insights to policy makers who are concerned about the huge gap between actual and potential collection of zakat and are considering tax reforms for incentivizing business zakat mobilization. It highlights the diversity in practices relating to zakat computation and related taxation across Muslim countries. Originality/value The paper searches for and observes consistency and compatibility between the orthodox Shariah-legal position and several accounting and taxation-related policies relating to business zakat. The policy prescriptions are expected to rejuvenate and strengthen the global zakat sector.
Imbalance of pro‐ and antifibrogenic genes and bile duct injury in murine Schistosoma mansoni infection–induced liver fibrosis

Objectives The murine model of Schistosoma mansoni infection is characterized by strong fibrosis and little hepatocellular injury. The objective of this study was to evaluate the potential link between hepatic schistosomiasis and bile duct injury in relation to the expression of profibrotic cytokines and fibrosis‐related genes.