Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcription-dependence of histone H3 lysine 27 trimethylation at the Arabidopsis polycomb target gene FLC.

The FLC gene encodes a MADS box repressor of flowering that is the main cause of the late-flowering phenotype of many Arabidopsis ecotypes. Expression of FLC is repressed by vernalization; maintenance of this repression is associated with the deposition of histone 3 K27 trimethylation (H3K27me3) at the FLC locus. However, whether this increased H3K27me3 is a consequence of reduced FLC transcription or the cause of transcriptional repression is not well defined. In this study we investigate the effect of changes in transcription rate on the abundance of H3K27me3 in the FLC gene body, a chromatin region that includes sequences required to maintain FLC repression following vernalization. We show that H3K27me3 is inversely correlated with transcription across the FLC gene body in a range of ecotypes and mutants with different flowering times. We demonstrate that the FLC gene body becomes marked with H3K27me3 in the absence of transcription. When transcription of the gene body is directed by an inducible promoter, H3K27me3 is removed following activation of transcription and H3K27me3 is added after transcription is decreased. The rate of addition of H3K27me3 to the FLC transgene following inactivation of transcription is similar to that observed in the FLC gene body following vernalization. Our data suggest that reduction of FLC transcription during vernalization leads to an increase of H3K27me3 levels in the FLC gene body that in turn maintains FLC repression.
A study comparing various noninvasive methods of detecting bladder cancer in urine

Objectives To compare the nuclear matrix protein (NMP)‐22 assay, bladder tumour specific antigen (BTAstat) test, telomerase activity (using the telomeric repeat amplification protocol assay, TRAP) and a haemoglobin dipstick test for their ability to replace voided urine cytology (VUC) for detecting bladder cancer.
Tumor protein 53 mutations and inherited cancer: beyond Li-Fraumeni syndrome

Purpose of review Germline TP53 (tumor protein 53) mutations are the molecular basis of a complex cancer predisposition syndrome, the Li-Fraumeni syndrome. The present review discusses the diversity of tumor patterns in TP53 mutation carriers, focusing on molecular factors that may explain familial and individual differences, such as genotype/phenotype correlations, genetic modifiers and genetic anticipation. Recent findings Initially identified 20 years ago, germline TP53 mutations appear to be associated with an extremely diverse range of cancers. Although no other gene has been found in Li-Fraumeni syndrome, recent results show that the functional effects of particular mutations, polymorphisms in TP53 or in regulators such as MDM2 (murine double minute 2), variations in DNA copy number and variations in telomere length, have a strong impact on individual risk and on tumor patterns. Furthermore, recent studies in large cohorts suggest that TP53 germline mutations may occur in up to 1: 5000 individuals. Summary Germline TP53 mutations may be responsible for a large fraction (15–20%) of all inherited cancers. Although mutations are detectable by sequencing, counseling and follow-up remain problematic due to the wide variations in disease presentation. Elucidating the molecular mechanisms underlying the predisposition caused by TP53 deficiency may help to develop better, evidence-based and personalized clinical protocols.
Human THAP7 Is a Chromatin-associated, Histone Tail-binding Protein That Represses Transcription via Recruitment of HDAC3 and Nuclear Hormone Receptor Corepressor*

The identities of signal transducer proteins that integrate histone hypoacetylation and transcriptional repression are largely unknown. Here we demonstrate that THAP7, an uncharacterized member of the recently identified THAP (Thanatos-associated protein) family of proteins, is ubiquitously expressed, associates with chromatin, and represses transcription. THAP7 binds preferentially to hypoacetylated (un-, mono-, and diacetylated) histone H4 tails in vitro via its C-terminal 77 amino acids. Deletion of this domain, or treatment of cells with the histone deacetylase inhibitor TSA, which leads to histone hyperacetylation, partially disrupts THAP7/chromatin association in living cells. THAP7 coimmunoprecipitates with histone deacetylase 3 (HDAC3) and the nuclear hormone receptor corepressor (NCoR) and represses transcription as a Gal4 fusion protein. Chromatin immunoprecipitation assays demonstrate that these corepressors are recruited to promoters in a THAP7 dependent manner and promote histone H3 hypoacetylation. The conserved THAP domain is a key determinant for full HDAC3 association in vitro, and both the THAP domain and the histone interaction domain are important for the repressive properties of THAP7. Full repression mediated by THAP7 is also dependent on NCoR expression. We hypothesize that THAP7 is a dual function repressor protein that actively targets deacetylation of histone H3 necessary to establish transcriptional repression and functions as a signal transducer of the repressive mark of hypoacetylated histone H4. This is the first demonstration of the transcriptional regulatory properties of a human THAP domain protein, and a critical identification of a potential transducer of the repressive signal of hypoacetylated histone H4 in higher eukaryotes.
Expression of recA in Deinococcus radiodurans

Deinococcus (formerly Micrococcus) radiodurans is remarkable for its extraordinary resistance to ionizing and UV irradiation and many other agents that damage DNA. This organism can repair > 100 double-strand breaks per chromosome induced by ionizing radiation without lethality or mutagenesis. We have previously observed that expression of D. radiodurans recA in Escherichia coli appears lethal. We now find that the RecA protein of D. radiodurans is ot detectable in D. radiodurans except in the setting of DNA damage and that termination of its synthesis is associated with the onset of deinococcal growth. The synthesis of Shigella flexneri RecA (protein sequence identical to that of E. coli RecA) in recA-defective D. radiodurans is described. Despite a large accumulation of the S. flexneri RecA in D. radiodurans, there is no complementation of any D. radiodurans recA phenotype, including DNA damage sensitivity, inhibition of natural transformation, or inability to support a plasmid that requires RecA for replication. To ensure that the cloned S. flexneri recA gene was not inactivated, it was rescued from D. radiodurans and was shown to function normally in E. coli. We conclude that neither D. radiodurans nor S. flexneri RecA is functional in the other species, nor are the kinetics of induction and suppression similar to each other, indicating a difference between these two proteins in their modes of action.
Red meat, dietary nitrosamines, and heme iron and risk of bladder cancer in the European Prospective Investigation into Cancer and Nutrition (EPIC).

BACKGROUND Previous epidemiologic studies found inconsistent results for the association between red meat intake, nitrosamines [NDMA: N-nitrosodimethylamine, and ENOC (endogenous nitroso compounds)], and the risk of bladder cancer. We investigated the association between red meat consumption, dietary nitrosamines, and heme iron and the risk of bladder cancer among participants of the European Prospective Investigation into Cancer and Nutrition (EPIC). METHODS Data on food consumption and complete follow-up for cancer occurrence were available for a total of 481,419 participants, recruited in 10 European countries. Estimates of HRs were obtained by proportional hazard models, stratified by age at recruitment, gender, and study center and adjusted for total energy intake, smoking status, lifetime intensity of smoking, duration of smoking, educational level, and BMI. RESULTS After a mean follow-up of 8.7 years, 1,001 participants were diagnosed with bladder cancer. We found no overall association between intake of red meat (log2 HR: 1.06; 95% CI: 0.99-1.13), nitrosamines (log2 HR: 1.09; 95% CI: 0.92-1.30 and HR: 0.98; 95% CI: 0.92-1.05 for ENOC and NDMA, respectively) or heme iron (log2 HR: 1.05; 95 CI: 0.99-1.12) and bladder cancer risk. The associations did not vary by sex, high- versus low-risk bladder cancers, smoking status, or occupation (high vs. low risk). CONCLUSIONS Our findings do not support an effect of red meat intake, nitrosamines (endogenous or exogenous), or heme iron intake on bladder cancer risk.
Autologous cultured keratinocytes on porcine gelatin microbeads effectively heal chronic venous leg ulcers

We have established a specific bioreactor microcarrier cell culture system using porcine gelatin microbeads as carriers to produce autologous keratinocytes on a large scale. Moreover, we have shown that autologous keratinocytes can be cultured on porcine collagen pads, thereby forming a single cell layer. The objective of this study was to compare efficacy and safety of autologous cultured keratinocytes on microbeads and collagen pads in the treatment of chronic wounds. Fifteen patients with recalcitrant venous leg ulcers were assigned to three groups in a single‐center, prospective, uncontrolled study: five underwent a single treatment with keratinocyte monolayers on collagen pads (group 1); another five received a single grafting with keratinocyte‐microbeads (group 2); and the last five received multiple, consecutive applications of keratinocyte‐microbeads 3 days apart (group 3). All patients were followed for up to 12 weeks. By 12 weeks, there was a mean reduction in the initial wound area of 50, 83, and 97 percent in the three groups, respectively. The changes in wound size were statistically significant between the first and third groups (p= 0.0003). Keratinocyte‐microbeads proved to be more effective than keratinocyte monolayers on collagen pads when the former were applied every 3 days. Rapid availability within 10–13 days after skin biopsy and easy handling represent particular advantages.
Sources and fate of freshwater exported in the East Greenland Current

Monitoring the sources and fate of freshwater in the East Greenland Current (EGC) is important, as this water has the potential to suppress deep convection in the Nordic and Labrador Seas if the outflow of freshwater from the Arctic Ocean increases in response to climate change. Here, hydrographic, oxygen isotope ratio and dissolved barium concentration sections across Denmark Strait collected in 1998 and 1999 are used to determine the freshwater composition of the EGC at these times. Comparison of meltwater fluxes at Denmark Strait and Fram Strait indicates a net melting of sea ice into the EGC between these two locations, with a significant proportion of sea ice drifting into the Nordic Seas or on to the East Greenland Shelf. We conclude that the phase of freshwater exiting the Arctic Ocean through Fram Strait is important in determining its possible impact on deep water formation in the Nordic and Labrador Seas.
Mechanical behaviour of Fe-C alloys during phase transformations

The mechanical behaviour of Fe-C alloys have been determined in their phase transformation ranges during isothermal or anisothermal transformations. Two models an analytical model and a finite element model have been used in order to calculate the behaviour of the material. Considering a macroscopic scale, these models show that the mechanical behaviour can be well described when an additional deformation, i.e. the transformation plasticity deformation, is considered
Rosenzweig Frustration Test to Assess Tolerance to Frustration and Direction of Aggressiveness in Criminals

The present study aimed to evaluate the tolerance to frustration and aggressive feelings of prisoners by the help of Rosenzweig Frustration Test (RFT). Besides, it also verified the relationship between the type of offense and aggressiveness direction. The study was approved by the Ethical Research Board Co mmittee of Sao Francisco University, followed by the volunteers signing an informed consent form, which faithfully observed all resolutions of the National Ministry of Health. The study included 125 prisoners of a heavily guarded prison in the state of Sao Paulo, Brazil. The age of participants ranged from 19 to 46 years (M = 29.8 years, SD = 6.4 years). The applicat ion of instruments was collective, into subgroups of approximately 20 subjects. A questionnaire was applied to characterize the sample and RFT, with an approximate duration of 1 hour. On investigating the extent to which the RFT factors differed fro m the groups, we observed significant differences between the groups that committed theft and the group that committed other offenses (theft x factors, Λ = 0.95, p = 0.05). Hence, it was concluded that there was a presence of low frustration tolerance in the sample of prisoners, especially for the group that committed theft, compared to the group that committed other types of offenses.
Vigilance and predator detection vary between avian species with different visual acuity and coverage

Interspecific variations in avian visual systems have been suggested to influence antipredator strategies, yet little empirical evidence exists on how morphological and ecological factors associated with visual properties can constraint predator detection. We investigated antipredator responses (predator detection probabilities and vigilance behavior) in 2 species with different visual properties (European starlings, Sturus vulgaris, have higher acuity and wider blind areas at the rear of their heads than house sparrows, Passer domesticus) in relation to distance to the predator, body posture, and head orientation. Visual acuity may affect the distance at which a predator is detected; while the size of the blind area may influence the body postures and head orientation with the highest predator detection probabilities. Distance to the decreased predator detection probabilities of house sparrows (lower acuity) but not those of European starlings. Certain body postures and head orientations, influenced both species despite the interspecific differences in visual field configuration. Times allocated to scanning were similar, but European starlings had longer head-up scan bouts likely to enhance scanning coverage, whereas house sparrows had higher head-up scan rates probably to reduce the length of interscan intervals. We discuss alternative interpretations; however, our findings suggest that sensory configurations may limit the effectiveness of some antipredator strategies under certain ecological conditions, which has implications for understanding the evolution of different behavioral mechanisms that reduce predation risk. Copyright 2009, Oxford University Press.
An Empirical Analysis of Scarcity Strategies in the Automobile Industry

Recent product introductions such as the Xbox 360, Sony Playstation 2, and PT Cruiser have been characterized by shortage of these products. Some experts have suggested that such scarcity can be a deliberate strategy for making the product more desirable. In this paper, we empirically examine the relationship between introductory inventory levels and consumer preference in the U.S. automobile industry and show that relative scarcity of a car at the time of introduction is associated with higher consumer preference for the product. Furthermore, we perform an empirical test of alternative theories about the rationale for introductory product scarcity. Specifically, we consider two theories of supplier-induced scarcity, namely the buying frenzy theory and the signaling theory, and an alternative theory that suggests that demand uncertainty causes introductory product scarcity. We find more support for the signaling theory of supplier-induced scarcity than the buying frenzy theory or the demand uncertainty theory in our analysis of the automobile market.
The anthelmintic properties of tannin-rich legume forages: from knowledge to exploitation in farm conditions

Tannin-rich legume forages have nutraceutical value for ruminants through slowing down the infec- tions with parasitic nematodes of the gastro intestinal tract. This review aims to describe the effects of tan- nin-containing legumes on the various nematode stages We will also discuss the sources of variability in results and present the current hypotheses in regard to the possible modes of action of the plant secondary metabolite on the worms.
Significance of nucleated red blood cells in peripheral blood; analysis of 1,496 cases.

Under normal conditions, nucleated red blood cells are found only in the circulating blood of the fetus and the newborn infant. Beyond that period the presence of nucleated red blood cells in the peripheral blood indicates disorder in the blood-producing mechanism. Because nucleated red blood cells are immature cells, they do not enter the blood normally. No totally satisfactory explanation is available of the precise mechanism that maintains a "barrier" that normally prevents primitive cells of the marrow from reaching the peripheral circulation. Evidence suggests at least two mechanisms: one chemical, primarily influenced by anoxia; the other physical, dependent at least in part on the syncytial arrangement of primitive cells. The violation of this "barrier" and the escape of nucleated red blood cells signal the presence of a stimulus that is allowing the release of these cells, before they have passed through the intermediate reticulocyte stage, to become adult red
While Dysferlin and Myoferlin Are Coexpressed in the Human Placenta, Only Dysferlin Expression Is Responsive to Trophoblast Fusion in Model Systems1

Abstract The syncytiotrophoblast is a specialized epithelium derived from mononuclear cytotrophoblasts that fuse to form this extensive syncytium. Dysferlin is expressed primarily in the apical plasma membrane of the syncytiotrophoblast in the human placenta. Here, we document the presence of another member of the ferlin family, myoferlin, in the placenta and show that it too is expressed primarily in the syncytiotrophoblast. Additionally, we examined the trophoblastic cell lines BeWo, JAR, and JEG-3 for the expression of dysferlin and myoferlin and determined the extent to which their expression was modulated by cell-cell fusion. In trophoblastic cells, there was a positive correlation between cell fusion and increased dysferlin expression but not myoferlin expression. Regarding expression, these trophoblastic cell lines recapitulate the distribution of dysferlin in mononuclear cytotrophoblasts and the syncytiotrophoblast in vivo.
Seroprevalence and Risk Factors of Hepatitis B Virus Infection among Adolescents in Enugu, Nigeria.

OBJECTIVES The aim of the study was to determine the prevalence and correlates of hepatitis B virus infection in adolescents in Enugu-urban. METHODS A cross-sectional seroprevalence survey was conducted among school children aged 10-18 years. Subjects were selected using multistage sampling. Hepatitis B surface antigenaemia (HBsAg) was assayed for in blood using rapid enzyme-linked immunosorbent assay kits. Data were analyzed using statistical package for social sciences version 16.0 (p < 0.05). RESULTS Four hundred and twenty children were recruited. Of these, 13 tested positive for HBsAg, giving an overall hepatitis B seroprevalence of 3.1%. Social class, scarifications/tattooing, circumcision and history of surgery were the significant modes of transmission. CONCLUSION The seroprevalence of hepatitis B among children in the study population is high. Its screening in school children should be incorporated into school health services in our setting while awareness campaigns and health education on its modes of transmission and prevention should be promoted and strengthened.
Fatal granulomatous amoebic encephalitis caused by Balamuthia mandrillaris

Granulomatous amoebic encephalitis caused by Balamuthia mandrillaris is rare (63 human cases reported worldwide) and fatal. We report a case in a five‐year‐old boy who had previously been well. For 18 months, he had had a slowly progressive, granulomatous mid facial lesion, but despite extensive investigation definitive diagnosis was made only with the acute onset of neurological signs in the last two weeks of life, when a brain biopsy specimen revealed amoebic trophozoites and cysts. Infection with B. mandrillaris should be considered in the differential diagnosis of chronic skin lesions with non‐specific granulomatous histopathology and negative microbiological test results.
Global software development using agile methodologies: A review of literature

This paper presents review of literature on global software development “G.S. D” and the use of agile methodologies in GSD. Three major themes were identified from extant literature on GSD: performance of global software development, governance related issues and software engineering process related issues. Further analysis of literature on agile methods and GSD showed that repertoire of methodologies spanning the various types of agile methods were applied related to agile methods that could be spliced or combined and tailored appropriately to produce optimal performance in the context of GSD. However, empirical studies addressing GSD-Agile fit were not found and as such require more attention in future studies.
HeartCare+: A Smart Heart Care Mobile Application for Framingham-Based Early Risk Prediction of Hard Coronary Heart Diseases in Middle East

Background. Healthcare is a challenging, yet so demanding sector that developing countries are paying more attention to recently. Statistics show that rural areas are expected to develop a high rate of heart diseases, which is a leading cause of sudden mortality, in the future. Thus, providing solutions that can assist rural people in detecting the cardiac risks early will be vital for uncovering and even preventing the long-term complications of cardiac diseases. Methodology. Mobile technology can be effectively utilized to limit the cardiac diseases’ prevalence in rural Middle East. This paper proposes a smart mobile solution for early risk detection of hard coronary heart diseases that uses the Framingham scoring model. Results. Smart HeartCare+ mobile app estimates accurately coronary heart diseases’ risk over 10 years based on clinical and nonclinical data and classifies the patient risk to low, moderate, or high. HeartCare+ also directs the patients to further treatment recommendations. Conclusion. This work attempts to investigate the effectiveness of the mobile technology in the early risk detection of coronary heart diseases. HeartCare+ app intensifies the communication channel between the lab workers and patients residing in rural areas and cardiologists and specialist residing in urban places.
Thymoquinone protects against carbon tetrachloride hetatotoxicity in mice via an antioxidant mechanism

Thymoquinone (TQ) is the major active component of the volatile oil of Nigella sativa seeds. The effects of TQ on carbon tetrachloride (CC14)‐induced hepatotoxicity was investigated in male Swiss albino mice. Carbon tetrachloride (20 μl/Kg, i.p.) injected into mice, induced damage to liver cells and was followed by the increase in serum alanine aminotransferase (ALT) activity after 24 h. Oral administration of TQ in a single dose (100 mg/Kg) resulted in significant (p<0.001) protection against the hepatotoxic effects of CCl4.