Miracle-dz/UniFAR-FTUs huggingface dataset:
Age-Related Changes in Quantitative Strain of Mouse Astrocytic Lamina Cribrosa and Peripapillary Sclera Using Confocal Microscopy in an Explant Model

Purpose The purpose of this study was to measure the full-field deformation response to IOP change in the peripapillary sclera (PPS) and astrocytic lamina cribrosa (ALC) of young and old mouse eyes ex vivo. Methods Thirty-eight transgenic reporter mice with green fluorescent protein–expressing astrocytes were studied at 2 to 4 months and 13 to 15 months old. The ALC and PPS of the explant eyes were imaged using laser scanning microscopy under controlled inflation from 10 to 30 mm Hg. Strains were estimated for the ALC and PPS from imaged volumes using digital volume correlation. Results ALC strains were significantly greater than zero nasal–temporally for both age groups (mean = 4.3% and 4.0%; each P ≤ 0.004) and significantly greater than zero in the inferior–superior direction for younger mice (P = 0.0004). Younger mice had larger ALC inferior–superior strains than older mice (P = 0.002). The ALC area and perimeter enlarged with inflation in both age groups, with a greater increase in younger than in older mice (all P ≤ 0.004). The ALC nasal–temporal diameter change was greater than inferior–superiorly, and younger mice had greater enlargement nasal–temporally than older. PPS maximum shear strain was greater in the older mice (P = 0.002). The axial lengths of older mice were 14% longer and the PPS was 16% thinner than younger mice (both P = 0.0003). Conclusions The behavior of the ALC in younger mice with inflation exhibited greater strains and enlargement of ALC area than older mice. Some strain measures in the PPS were greater in older mice, likely related to their longer axial length and thinner PPS.
Periodontal Health Status and Pregnancy Outcomes: A Survey in Medical Doctors

ABSTRACT Introduction Periodontal disease and caries are the most common causes of tooth loss worldwide. Studies have demonstrated strong association between periodontitis and adverse pregnancy outcomes. Medical doctors, who are the primary healthcare providers, seldom advise women to seek dental care during pregnancy. This study was undertaken to explore the knowledge, attitudes and behaviours of medical doctors towards oral health and to identify the barriers of prenatal periodontal healthcare in their practices and its possible implications on pregnancy outcomes. Methods Total 377 doctors filled the questionnaire. The data collected through personal contacts, social networking, emails, online forms and networking at conferences were analysed using Statistical Package for the Social Sciences 20 software program and presented in tables, charts and diagrams. Results Out of 263 (69.8%) male and 114 (30.2%) female doctors enrolled in the study, only 52 (13.8%) had received education or training on oral care during pregnancy. Among them 299 (79.3%) agreed that there is possible link between health of teeth-gums and pregnancy. Approximately 105 (27.9%) encountered patients with oral/periodontal problem every week but only 108 (28.6%) ‘always’ advised their patient for regular dental check–ups. Similarly, 358 (95%) agreed that there is need for universal guidelines. However, 133 (35.3%) thought there was insufficient time to advise patients on oral health during check-ups. Conclusions There is need for training on ‘oral healthcare during pregnancy’ for medical doctors. Developing universal guidelines for oral healthcare in pregnant women for all health professionals would be another important step. An adequate referral system to oral healthcare providers and biannual check-ups is recommended for both general patient as well as pregnant women for preventing adverse situations related to oral and specifically periodontal diseases.
Stability of Values for the Activities of Critical Enzymes Assayed in Serum Frozen for Prolonged Time Periods.

OBJECTIVES Our medical center laboratory receives frozen clinical chemistry samples from outlying hospitals for which assays for critical enzyme activities are requested. Our objective is to determine the effects of freezing (-20°C) on these enzyme activities in samples over a one month period. METHODS Enzyme activities for ALP, AST, ALT, CK and LD for 30 patient's sera were stored at-20°C and were assayed on a Beckman-Coulter AU5800 analyzer at 0, 15 and 30 days after collection. Statistical tests were performed to determine if the values were statistically the same or different. RESULTS F-tests for all five enzyme levels showed no statistically significant differences (p>>0.05); linear regression analysis showed high correlation of results (r>0.99 for all correlations) with some bias for ALT. CONCLUSIONS We conclude that the activities of these enzymes are stable, except possibly ALT, when stored frozen at -20°C over the 15- and 30-day storage periods.
Evaluation on Directed Functional Brain Connectivity during the Expert Rifle Pre-shot Period

Abstract Experts require long-term and intense practice to acquire extraordinary motor skills that be known as the brain function regulator. However, the brain function network mechanism of rifle shooters is still unclear. The generalized orthogonalized partial directed coherence (gOPDC) algorithm and local efficiency significance analysis method (LESA) was applied to quantify the difference in directed functional networks between expert and novice rifle shooters during the pre-shot period. The more directed functional connections were observed in alpha and low-beta frequency bands as compared to other bands. Moreover, comparing with the novice’s fluctuant connection, the values of connection (P3→C3) strength were increasing steadily in the experts during the pre-shot period. Fewer connections in left hemisphere networks were obtained in the experts than in the novices. The results validated the “neural efficiency” hypothesis in experts. Moreover, the strength of the functional connection (P3→C3) in the alpha and beta bands serves as a distinguishing feature between experts and novices.
Exercise type relates to inhibitory and error processing functions in older adults

ABSTRACT This study investigated the association between exercise type and inhibition of prepotent responses and error detection. Totally, 75 adults (M = 68.88 years) were classified into one of three exercise groups: those who were regular participants in open- or closed-skill forms of exercise, and those who exercised only irregularly. The participants completed a Stroop and task-switching tasks with event-related brain potentials (ERPs) recorded. The results revealed that regular exercisers displayed faster reaction times (RTs) in the Stroop task compared with irregular exercisers. The open-skill exercisers exhibited smaller N200 and larger P300a amplitudes in the Stroop task compared with irregular exercisers. Furthermore, the open-skill exercisers showed a tendency of shorter error-related negativity latencies at the task-witching test. The findings suggest that older adults may gain extra cognitive benefits in areas such as inhibition functioning and error processing from participating in open-skill forms of physical exercises.
All‐trans retinoic acid reverses epithelial‐mesenchymal transition in paclitaxel‐resistant cells by inhibiting nuclear factor kappa B and upregulating gap junctions

Paclitaxel is a widely used chemotherapy drug, but development of resistance leads to treatment failure. Tumor cells that are treated with a sublethal dose of paclitaxel for a long period of time show the epithelial‐mesenchymal transition (EMT) phenotype, which leads to metastasis and resistance. All‐trans retinoic acid (ATRA) is always used in combination with paclitaxel and can reverse EMT in many types of cancer cells. The ability of ATRA to reverse EMT in chemoresistant cells is still unknown. In the present study, the ability of ATRA to reverse EMT in paclitaxel‐resistant cells was investigated. Three colorectal cancer cell lines, HCT116, LoVo and CT26, were treated with sublethal doses of paclitaxel to create resistant cell lines. Western blotting, immunocytochemistry, and “parachute” dye‐coupling assays showed that ATRA reverses EMT, inhibits nuclear factor kappa B (NF‐κΒ), and upregulates gap junctions in paclitaxel‐resistant cells. Scratch wound‐healing and Transwell assays showed that ATRA decreases the migration and invasion abilities of paclitaxel‐resistant cells. In addition, the CT26 cell line was used in the Balb/c pulmonary metastasis model to show that ATRA reduces metastasis of paclitaxel‐resistant cells in vivo. Given these data, ATRA may reverse EMT by inhibiting NF‐κΒ and upregulating gap junctions in paclitaxel‐resistant cells.
Clinical and pathophysiological aspects of bicuspid aortic valve disease

Abstract A bicuspid aortic valve is not only a common congenital heart defect but also an enigmatic condition that can cause a large spectrum of diseases, such as aortic valve stenosis and severe heart failure in newborns whereas aortic dissection in adults. On the contrary, a bicuspid aortic valve can also occur with normal function throughout life and never need treatment. Numerous genetic mechanisms are involved in the abnormal cellular functions that may cause abnormal development of the aortic valve during early foetal life. As several chromosomal disorders are also associated with a bicuspid valve, there does not appear to be an apparent common trigger to the abnormal development of the aortic valve. The clinical care of the bicuspid aortic valve patient has been changed by a significant body of evidence that has improved the understanding of the natural history of the disease, including when to best intervene with valve replacement and when to provide prophylactic aortic root surgery. Moreover, as bicuspid valve disease is also part of various syndromes, we can identify high-risk patients in whom a bicuspid valve is much more unfavourable than in the normal population. This review provides an overview of all aspects of the bicuspid aortic valve condition and gives an updated perspective on issues from pathophysiology to clinical care of bicuspid aortic valve disease and associated aortic disease in asymptomatic, symptomatic, and pregnant patients, as well as our viewpoint on population screening.
Daily rhythms of leukocytes populations, biochemical and enzymatic blood parameters in a carnivorous freshwater catfish (Lophiosilurus alexandri)

ABSTRACT The aim of this study was to investigate the daily rhythms of hematological, biochemical and enzymatic parameters of the blood of a nocturnal model of fish (Lophiosilurus alexandri) bred in the laboratory (F1). Thirty-six juveniles were stocked in six tanks of a recirculation aquaculture system for 20 days. The fish were exposed to a light:dark cycle of 12:12 h and were fed 1% of biomass twice a day with commercial diet. The daily rhythms of hematological, biochemical and enzymatic parameters were then measured at six sampling times “zeitgeber time = ZT” at four-hour intervals under light:dark 12:12 h (lights on = ZT0, at 8.00 a.m). No differences were observed to alkaline phosphatase, glucose, cortisol, aspartate aminotransferase, superoxide dismutase, total protein and hematocrit (p > 0.05). However, white blood cell count, Lymphocytes (LYN), Neutrophils (NEU), Eosinophil and Neutrophils to Lymphocytes ratio were significant different between sample times (p < 0.05). Also, a significant difference in alanine transaminase was observed, with a peak of production at nighttime. In contrast, glutathione peroxidase peaked at 8:00. Uric acid, magnesium and Calcium (Ca++) showed statistically significant differences (p < 0.05). A significant difference was observed (p < 0.05), with a peak of albumin at 08:00 and triglycerides at 12:00, while cholesterol was low (p < 0.05) at 08:00 and higher from 12:00 to 04:00. Cosinor analysis revealed also rhythmicity to SOD, UA, Mg and Ca++, ALB and CHO (p < 0.05). In conclusion, the time of day must be considered a key factor when using blood parameters as biomarkers for disease, health and welfare in the L. alexandri aquaculture.
Structured Content Preservation for Unsupervised Text Style Transfer

Text style transfer aims to modify the style of a sentence while keeping its content unchanged. Recent style transfer systems often fail to faithfully preserve the content after changing the style. This paper proposes a structured content preserving model that leverages linguistic information in the structured fine-grained supervisions to better preserve the style-independent content during style transfer. In particular, we achieve the goal by devising rich model objectives based on both the sentence's lexical information and a language model that conditions on content. The resulting model therefore is encouraged to retain the semantic meaning of the target sentences. We perform extensive experiments that compare our model to other existing approaches in the tasks of sentiment and political slant transfer. Our model achieves significant improvement in terms of both content preservation and style transfer in automatic and human evaluation.
HPLC-UV/Vis-APCI-MS/MS Determination of Major Carotenoids and Their Bioaccessibility from “Delica” (Cucurbita maxima) and “Violina” (Cucurbita moschata) Pumpkins as Food Traceability Markers

Carotenoids are a widespread group of fat-soluble pigments, and their major nutritional importance comes from their pro-vitamin A activity and their antioxidant capacity. In this study, two different pumpkin cultivars (Cucurbita maxima, also named ‘Delica’ and Cucurbita moschata, also known as ‘Violina’) from the southern Po Delta area were investigated in terms of carotenoid content and the influence of food processing on compositional changes and carotenoid bioaccessibility. Quali- and quantitative determination of carotenoids in sample extracts were performed on a C30 column by means of an online coupled HPLC-UV/Vis-APCI-MS/MS technique. The identification of separated compounds was tentatively achieved by merging (i) chromatographic data, (ii) UV-Vis spectra, and (iii) MS/MS fragmentation spectra. The chromatographic profiles for the two cultivars showed qualitative differences. Two major carotenoids were considered for quantification purposes and further investigations: lutein and β-carotene. Quantification of target carotenoids was performed with external calibration through analytical standards. The concentration of lutein and β-carotene was higher in C. maxima than in the other variety, C. moschata. Carotenoids are susceptible to degradation (isomerization and oxidation) during food processing (i.e., cooking), and the concentration of lutein and β-carotene were monitored in oven-cooked and steam-cooked pumpkins. The steam-cooking process was superior in terms of limiting carotenoid loss. A complete functional profile of pumpkins as a source of carotenoids was gained with the evaluation of their in vitro bioaccessibility and their bioavailability after intake during human digestion. Bioaccessibility of lutein and β-carotene were estimated by an in vitro static digestion model that involved salivary, gastric, and duodenal phases. Bioaccessibility values progressively increased from the salivary to the duodenal phase for both pumpkin varieties and cooking methods. Bioaccessibility of lutein was always lower than β-carotene for both cultivars and for both cooking methods. Bioaccessibility values for lutein and β-carotene changed from 1.93% to 2.34% vs. 4.94% and 8.83% in the salivary phase, from 2.7% to 4.63% vs. 7.83% and 15.60% in the gastric phase, and from 10.04% to 13.42% vs. 25.81% and 35.32% in the duodenal phase. For both target compounds, bioaccessibility in the duodenal phase was more than twice the gastric values, and it underlined that the type of cooking did not influence release from the initial matrix.
Hybrid Knowledge Routed Modules for Large-scale Object Detection

The dominant object detection approaches treat the recognition of each region separately and overlook crucial semantic correlations between objects in one scene. This paradigm leads to substantial performance drop when facing heavy long-tail problems, where very few samples are available for rare classes and plenty of confusing categories exists. We exploit diverse human commonsense knowledge for reasoning over large-scale object categories and reaching semantic coherency within one image. Particularly, we present Hybrid Knowledge Routed Modules (HKRM) that incorporates the reasoning routed by two kinds of knowledge forms: an explicit knowledge module for structured constraints that are summarized with linguistic knowledge (e.g. shared attributes, relationships) about concepts; and an implicit knowledge module that depicts some implicit constraints (e.g. common spatial layouts). By functioning over a region-to-region graph, both modules can be individualized and adapted to coordinate with visual patterns in each image, guided by specific knowledge forms. HKRM are light-weight, general-purpose and extensible by easily incorporating multiple knowledge to endow any detection networks the ability of global semantic reasoning. Experiments on large-scale object detection benchmarks show HKRM obtains around 34.5% improvement on VisualGenome (1000 categories) and 30.4% on ADE in terms of mAP. Codes and trained model can be found in this https URL.
Enhancing Our Understanding of Teachers' Personal Responsibility for Student Motivation: A Mixed Methods Study

As measured by the Teachers Responsibility Scale, teachers appear to have surprisingly low levels of personal responsibility for student motivation even though they qualitatively identify low student motivation as a major teaching concern. Thus, the purpose of the current mixed methods research was to compare the way teachers’ respond to items about personal responsibility for student motivation quantitatively and qualitatively. We used a convergent sequential mixed method design to answer the following research question: How do practicing teachers’ perceptions and experiences of being personally responsible for student motivation converge with a quantitative measure of the construct? One hundred and 80 practicing teachers completed a self-report questionnaire on personal responsibility and then six teachers were purposefully sampled to participate in small-group interviews sharing their perspectives on responsibility for motivation specifically. The quantitative and qualitative data were analyzed separately and then integrated through a qualitative dominant crossover mixed analyses. Five mixed insights emerged and are represented in a joint display: dominance of interest, shared responsibility, divergent specificity and valence, complete alignment, and missingness of professional perspective. The mixed insights have important implications for theory, research, and practice and highlight the contribution that mixed methods can have in advancing motivation research.
A review of factors affecting patient fertility preservation discussions & decision‐making from the perspectives of patients and providers

Women undergoing cancer treatments and their healthcare providers encounter challenges in fertility preservation (FP) discussions and decision-making. A systematic review of qualitative research was conducted to gain in-depth understanding of factors influencing FP discussions and decision-making. Major bibliographic databases and grey literature in English from 1994 to 2016 were searched for qualitative research exploring patient/provider perspectives on barriers and facilitators to FP decision-making. Two researchers screened article titles, abstracts and full-texts. Verbatim data on research questions, study methodology, participants, findings and discussions of findings were extracted. Quality assessment and thematic analysis were conducted. The search yielded 74 studies dating from 2007 onwards; 29 met the inclusion criteria. Analysis revealed three types of barriers: (a) FP knowledge, skills and information deficits contributed to discomfort for providers and discontent for patients; (b) psychosocial factors and clinical issues influenced providers' practices around FP discussions and patients' decision-making; and (c) material, social and structural factors (e.g., lack of resources and accessibility) posed challenges to FP discussions. Potential facilitators to FP discussions and decision-making were also identified. A discussion of ways to improve physician's knowledge and facilitate women's decision-making and access to FP is presented, along with areas for policy development and further research.
Student understanding of graph slope and area under a graph: A comparison of physics and nonphysics students

Ana Susac, Andreja Bubic, Elizabeta Kazotti, Maja Planinic, and Marijan Palmovic Department of Applied Physics, Faculty of Electrical Engineering and Computing, University of Zagreb, Unska 3, 10000 Zagreb, Croatia Department of Physics, Faculty of Science, University of Zagreb, Bijenicka 32, 10000 Zagreb, Croatia Chair for Psychology, Faculty of Humanities and Social Sciences, University of Split, Sinjska 2, 21000 Split, Croatia Laboratory for Psycholinguistic Research, Department of Speech and Language Pathology, University of Zagreb, Borongajska cesta 83h, 10000 Zagreb, Croatia
Calcineurin promotes APC/C activation at meiotic exit by acting on both XErp1 and Cdc20

Vertebrate oocytes await fertilization arrested at metaphase of the second meiotic division. Fertilization triggers a transient calcium wave, which induces the activation of the anaphase‐promoting complex/cyclosome (APC/C) and its co‐activator Cdc20 resulting in the destruction of cyclin B and hence meiotic exit. Two calcium‐dependent enzymes are implicated in fertilization‐induced APC/CCdc20 activation: calcium‐/calmodulin‐dependent kinase type II (CaMKII) and calcineurin (CaN). While the role of CaMKII in targeting the APC/C inhibitor XErp1/Emi2 for destruction is well‐established, it remained elusive how CaN affects APC/CCdc20 activation. Here, we discover that CaN contributes to APC/CCdc20 activation in Xenopus laevis oocytes by two independent but interrelated mechanisms. First, it facilitates the degradation of XErp1 by dephosphorylating it at a site that is part of a phosphorylation‐dependent recruiting motif for PP2A‐B′56, which antagonizes inhibitory phosphorylation of XErp1. Second, it dephosphorylates Cdc20 at an inhibitory site, thereby supporting its APC/C‐activating function. Thus, our comprehensive analysis reveals that CaN contributes to timely APC/C activation at fertilization by both negatively regulating the APC/C inhibitory activity of XErp1 and positively regulating the APC/C‐activating function of Cdc20.
The chemokine system and its role in obesity

The chemokine system is a complex arrangement of molecules that attract leukocytes to the site of injury or inflammation. This chemotactic behavior gives the system the name “Chemokine. ” The intricate and redundant nature of the chemokine system has made it a subject of ongoing scientific investigation. Obesity is characterized as low‐grade systemic or chronic inflammation that is responsible for the release of cytokines, adipokines, and chemokines. Excessive tissue fat expansion triggers the release of chemokines, which in turn attract various leukocytes and activate the resident immune surveillance system, eventually leading to worsening of obesity and other related comorbidities. To date, 50 chemokines and 20 chemokine receptors that belong to the G‐protein‐coupled receptor family have been discovered, and over the past two decades, the physiological and pathological roles of many of these chemokines and their receptors have been elucidated. The objective of this review is to present an update on the link between chemokines and obesity under the light of recent knowledge.