Miracle-dz/UniFAR-FTUs huggingface dataset:
Experimental Physiology - Research Paper Matching of blood flow to metabolic rate during recovery from moderate exercise in humans

It is unclear whether measurement of limb or conduit artery blood flow during recovery from exercise provides an accurate representation of flow to the muscle capillaries where gas exchange occurs. To investigate this, we: (a) examined the kinetic responses of femoral artery blood flow ( ˙ Q FA), estimated muscle capillary blood flow ( ˙ Q cap) and estimated muscle oxygenuptake( ˙ VO2m)followingcessationofexercise;and(b) comparedtheseresponsestoverify the adequacy of O2 delivery during recovery. Pulmonary ˙ VO2 ( ˙ VO2p) was measured breath by breath, ˙ Q FA wasmeasuredusingDopplerultrasonography,anddeoxy-haemoglobin/myoglobin (deoxy-[Hb/Mb]) was estimated by near-infrared spectroscopy over the rectus femoris in nine healthy subjects during a series of transitions from moderate knee-extension exercise to rest. The time course of ˙ Q cap was estimated by rearranging the Fick equation [i.e. ˙ Q cap(t) ∝ ˙ VO2m(t)/deoxy−[Hb/Mb](t)], using the primary component of ˙ VO2p to represent ˙ VO2m and deoxy-[Hb/Mb] as a surrogate for arteriovenous O2 difference. There were no significant differences among the overall kinetics of ˙ VO2m (τ, 31.4 ±8.2 s), ˙
Effect of antibiotics and BAU-biofungicide in controlling bacterial leaf blight of litchi

The experiment was carried out in the net house, Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh during 16 July 2012 to June 2013 to find out the suitable management practices for controlling bacterial leaf blight of litchi by applying six treatments viz., (i) T1–Gentamycin @ 0.05%, (ii) T2– Erithromycin @ 0.05%, (iii) T3–Doxycycline @ 0.05%, (iv) T4–Copper sulphate @ 0.05%, (v) T5–BAU-biofungicide @ 2% and (vi) T6–Control. Per cent incidence and per cent severity have been found to decrease by applying BAU-biofungicide, copper sulphate and gentamycin, although other treatments showed good result in decreasing severity of bacterial leaf blight. BAU-biofungicide was found superior among the treatments as it increased 18.83% plant height, 43.50% branch over control and reduced 17.16% disease incidence and 63.03% disease severity over control when applied as foliar spray @ 2%. Copper sulphate reduced 13.85% disease incidence and 50.21% disease severity over control and graded second best control measure for bacterial leaf blight of litchi when applied as foliar spray @ 0.05%.
Correlación y concordancia entre el examen nacional de medicina y el promedio ponderado universitario: análisis de la experiencia peruana en el periodo 2007 - 2009

Objectives: To evaluate the correlation and concordance between the ‘Peruvian National Exam of Medicine’ (ENAM) and the Mean Grade Point Average (GPA) in recently graduated medical students in the period 2007 to 2009. Materials and Methods: We carried out a secondary data analysis, using the records of the physicians applying to the Rural and Urban Marginal Service in Health of Peru (SERUMS) processes for the years 2008 to 2010. We extracted from these registers, the grades obtained in the ENAM and GPA. We performed a descriptive analysis using medians and 1 st and 3 rd quartiles (q1/q3); we calculated the correlation between both scores using the Spearman correlation coefficient, additionally, we conducted a lineal regression analysis, and the concordance was measured using the Bland and Altman coefficient. Results: A total of 6 117 physicians were included, the overall median for the GPA was 13.4 (12.7/14.2) and for the ENAM was 11.6 (10.2/13.0). Of the total assessed, 36.8% failed the TEST. We observed an increase in annual median of ENAM scores, with the consequent decrease in the difference between both grades. The correlation between ENAM and PPU is direct and moderate (0.582), independent from the year, type of university management (Public or Private) and location. However, the concordance between both ratings is regular, with a global coefficient of 0.272 (CI 95%: 0.260 to 0.284). Conclusions: Independently of the year, location or type of university management, there is a moderate correlation between the ENAM and the PPU; however, there is only a regular concordance between both grades.
Incidence of Footpad Dermatitis and Hock Burns in Broilers as Affected by Genotype, Lighting Program and Litter Type

Abstract The aim of this study was to define the conditions for improving broiler leg health, thus ultimately promoting broiler welfare and greater economic efficiency of production, by investigating the effects of litter type, lighting program, or genetic predisposition on the incidence and severity of footpad dermatitis and hock burns. The study was conducted on broiler genotypes (G) Hubbard Classic and Ross 308. Litter (L) was either chopped straw or wood shavings. Lighting programs (LP) were applied when broilers were eight days old. One lighting program (LP1) was designed so that chicks between 8 to 39 days of age were exposed to 16L:4D:2L:2D, and received 23L:1D between 40 to 42 days of age. The other lighting program (LP2) was a gradual extension of photoperiod after restrictions in the second week: 16L:8D (8-14 days), 16L:3D:2L:3D (15-21 days), 16L:2D:4L:2D (22-28 days), 16L:1D:6L:1D (29-35 days) and 23L:1D (36-42 days). Evaluations of footpad lesions and hock burn, according to the method described by Thomas et al. (2004), were carried out individually for each bird, on days 21 and 42. Based on the average score and the frequency of the worst forms of footpad dermatitis, better growing conditions for broilers were found when wood shavings were used as litter material rather than the chopped straw (P<0.01), and when a lighting program with gradual lengthening of photoperiod and with intermittent light/ dark periods (LP2) was applied, compared to a moderate-constant photoperiod lighting program (LP1) (P<0.01). The Ross 308 broiler genotype, despite its lower body weight, showed a greater tendency to develop footpad dermatitis and hock burns. Hock burns developed more slowly than footpad dermatitis, and thereby, would be likely to exhibit a smaller adverse effect on broiler welfare and quality of chicken feet.
In Vitro Antihyperlipidemic Potential of Triterpenes from Stem Bark of Protorhus longifolia

Abstract Two lanostane triterpenes, 3β-hydroxylanosta-9,24-dien-21-oic acid (1) and methyl-3β-hydroxylanosta-9,24-dien-21-oate (2), were isolated from the stem bark of Protorhus longifolia. Their structures were deduced on the basis of spectroscopic analysis (NMR, HRMS, IR). This study investigated the in vitro anti-adipogenic activity of the two triterpenes. Their inhibitory activity was evaluated on selected lipid digestive enzymes (pancreatic lipase and cholesterol esterase). The inhibitory activity of the compounds on hormone-sensitive lipase and their ability to bind bile acids were also evaluated. The effect of the compounds on glucose uptake in C2C12 muscle cells and 3T3-L1 adipocytes, and on triglyceride accumulation in 3T3-L1 adipocytes was investigated. The triterpenes effectively inhibited the activities of the enzymes with IC50 values ranging from 0.04 to 0.31 mg/mL. The compounds showed a high affinity for secondary bile acids. Both compounds stimulated glucose uptake in C2C12 muscle cells and 3T3-L1 adipocytes. Compound 1 significantly reduced triglyceride accumulation in mature differentiated 3T3-L1 adipocytes. It is apparent that these lanostane triterpenes enhance glucose uptake and suppress adipogenesis, which together with their inhibitory effects on lipid digestive enzymes suggests that they have antihyperlipidemic potential.
Naloxone does not inhibit the attenuation of the response to severe haemorrhage seen after simulated injury in the anaesthetized rat

Severe haemorrhage leads to a reflex bradycardia and hypotension. This is thought to be protective, but is attenuated by both concomitant musculoskeletal injury and exogenous morphine. The aim of this study was to determine whether the injury‐induced attenuation of the response to severe haemorrhage could be blocked by naloxone. Male Wistar rats, terminally anaesthetized with alphadolone/alphaxalone (19–20 mg kg−1 h−1i.v.), were randomly allocated to one of four groups. In groups I and IV, haemorrhage was simple [40% of estimated total blood volume (BV)], while in groups II and III it was initiated 10 min after the onset of bilateral hindlimb ischaemia (a model of musculoskeletal injury). Groups I and II received 20 μl of 0.9% saline intracerebroventricularly (i.c.v.) immediately before haemorrhage, while groups III and IV received 20 μg of naloxone i.c. v. , in the same volume. In group I, the bradycardia reached its peak after the loss of 32.8 ± 0.3% BV (mean ±s.e.m.). Blood pressure did not fall significantly until the loss of 15.0 ± 3.0% BV. The response in group IV was not significantly different from group I. By contrast, the bradycardia was absent after similar blood losses in groups II and III, while hypotension was attenuated. These results indicate that naloxone, at a dose known to be effective in blocking μ‐opioid receptors and preventing other aspects of the response to injury, does not prevent the injury‐induced attenuation of the response to severe haemorrhage. Thus the attenuation of the response to blood loss by injury is unlikely to be mediated via the μ‐opioid receptors.
Crystal structure of histone-like protein from Streptococcus mutans refined to 1.9 Å resolution.

Nucleoid-associated proteins (NAPs) in prokaryotes play an important architectural role in DNA bending, supercoiling and DNA compaction. In addition to architectural roles, some NAPs also play regulatory roles in DNA replication and repair, and act as global transcriptional regulators in many bacteria. Bacteria encode multiple NAPs and some of them are even essential for survival. Streptococcus mutans, a dental pathogen, encodes one such essential NAP called histone-like protein (HLP). Here, the three-dimensional structure of S. mutans HLP has been determined to 1.9 Å resolution. The HLP structure is a dimer and shares a high degree of similarity with other bacterial NAPs, including HU. Since HLPs are essential for the survival of pathogenic streptococci, this structure determination is potentially beneficial for future drug development against these pathogens.
Complex cardiovascular actions of α‐adrenergic receptors expressed in the nucleus tractus solitarii of rats

Although both α1‐ and α2‐adrenergic receptors (ARs) are known to be expressed in the nucleus of the solitary tract (NTS), the functional significance of these receptors is still not fully established. In this study, we microinjected α1‐ and α2‐AR agonists into the NTS of urethane‐anaesthetized Wister rats to study the cardiovascular effects in response to their activation. When the α1‐AR agonist phenylephrine was microinjected into the area where barosensitive neurons are dominantly located (baro‐NTS), mean arterial pressure (MAP) and heart rate (HR) were significantly elevated. When tested in the area where chemosensitive neurons are dominantly located (chemo‐NTS), however, MAP and HR were significantly decreased. Pretreatment with the non‐specific α‐AR antagonist phentolamine into the NTS inhibited the phenylephrine‐induced cardiovascular responses. In contrast, microinjection of the α2‐AR agonist clonidine into either the baro‐NTS or the chemo‐NTS decreased MAP and HR; they were also inhibited by the α2‐adrenergic antagonist yohimbine. Moreover, we immunohistochemically identified that cardiovascular responses induced by α1‐ARs may be mediated by NTS neurons while those induced by α2‐ARs may be mediated by astrocytes located in the barosensitive and chemosensitive areas of the NTS. These results suggest that both types of α‐AR expressed in the NTS may be involved in regulating cardiovascular homeostasis via modulation of input signals from baroreceptor and chemoreceptor afferents; however, cardiovascular responses produced by stimulation of α1‐ARs are strictly location specific within the NTS.
Occlusal status in orthodontically treated and untreated adolescents

In order to evaluate the outcome of orthodontic care, all available 16-year-old adolescents (n = 138) living within the catchment area of one Finnish health center were invited for a clinical examination. A total of 126 adolescents participated, corresponding to 79% of the whole age cohort. Of these, 47% had been treated orthodontically, 2% were still wearing appliances, and 51% were untreated. In 80% of cases, a non-extraction treatment was carried out. All occlusions were evaluated using a recently developed occlusal morphology and functional index (OMFI) based on assessment of 6 morphological and 4 functional traits which are classified applying an acceptable-non-acceptable dichotomy. The occlusal status in untreated individuals was used as a second reference in comparisons between the treated and untreated occlusions. The morphological criteria for an acceptable occlusion were met by 42% of the participants and the functional criteria by 64%. Although the treated occlusions failed to meet the criteria of morphological acceptability more often than the untreated ones (73% vs 40%), the proportions of non-acceptable occlusions were fairly high among untreated occlusions. The main reasons for non-acceptability were the failure of the canine relationship, overbite, and protrusion movement to fulfill the criteria of acceptability. Only 19% of the adolescents had occlusions classified as acceptable, both morphologically and functionally. In the evaluated health center, the allocation of resources resulted in a high coverage of orthodontic care. However, our results indicate that the general occlusal status in the examined age cohort did not reach an optimal level.
Comparative characterization of the glycosylation profiles of an influenza hemagglutinin produced in plant and insect hosts

The main objective of this study was to characterize the N‐linked glycosylation profiles of recombinant hemagglutinin (HA) proteins expressed in either insect or plant hosts, and to develop a mass spectrometry based workflow that can be used in quality control to assess batch‐to‐batch reproducibility for recombinant HA glycosylation. HA is a surface glycoprotein of the influenza virus that plays a key role in viral infectivity and pathogenesis. Characterization of the glycans for plant recombinant HA from the viral strain A/California/04/09 (H1N1) has not yet been reported. In this study, N‐linked glycosylation patterns of the recombinant HAs from both insect and plant hosts were characterized by precursor ion scan‐driven data‐dependent analysis followed by high‐resolution MS/MS analysis of the deglycosylated tryptic peptides. Five glycosylation sites (N11, N23, N276, N287, and N481) were identified containing high mannose type glycans in plant‐expressed HAs, and complex type glycoforms for the insect‐expressed HA. More than 95% site occupancy was observed for all glycosylation sites except N11, which was 60% occupied. Multiple‐reaction monitoring based quantitation analysis was developed for each glycopeptide isoform and the quantitative results indicate that the Man8GlcNAc2 is the dominant glycan for all sites in plant‐expressed HAs. The relative abundance of the glycoforms at each specific glycosylation site and the relative quantitation for each glycoform among three HAs were determined. Few differences in the glycosylation profiles were detected between the two batches of plant HAs studied, but there were significant differences between the glycosylation patterns in the HAs generated in plant and insect expression hosts.
Epidemiologia da leishmaniose tegumentar americana e suas relações com a lavoura e o garimpo, em localidade do Estado da Bahia (Brasil)

Realizou-se um estudo sobre a associacao entre infeccao leishmaniotica e a ocupacao dos individuos em Lencois-Bahia (Brasil). Foram utilizados um questionario com variaveis biologicas, sociais e economicas e o teste de Montenegro. Houve captura de flebotomineos em locais peri e intra-domiciliares. Encontrou-se maior prevalencia da infeccao no grupo ocupacional de lavradores/garimpeiros em comparacao as demais ocupacoes. Explica-se esse resultado em razao da dupla exposicao dos lavradores/garimpeiros a leishmaniose tegumentar americana, em sua area de moradia e local de trabalho.
Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose reductase (RfbD).

Bacillus anthracis is the causative agent of the deadly disease Anthrax. Its use in bioterrorism and its ability to re-emerge have brought renewed interest in this organism. B. anthracis is a Gram-positive bacterium that adds L-rhamnose to its cell-wall polysaccharides using the activated donor dTDP-β-L-rhamnose. The enzymes involved in the biosynthesis of the activated donor are absent in humans, which make them ideal targets for therapeutic development to combat pathogens. Here, the 2.65 Å resolution crystal structure of the fourth enzyme in the dTDP-β-L-rhamnose-biosynthetic pathway from B. anthracis, dTDP-4-dehydro-β-L-rhamnose reductase (RfbD), is presented in complex with NADP+. This enzyme catalyzes the reduction of dTDP-4-dehydro-β-L-rhamnose to dTDP-β-L-rhamnose. Although the protein was co-crystallized in the presence of Mg2+, the protein lacks the conserved residues that coordinate Mg2+.
Increased Protein and mRNA Expression of Resistin After Dexamethasone Administration

Abstract Synthetic glucocorticoids such as dexamethasone are widely used to treat a variety of inflammatory and autoimmune conditions, but they may induce adverse events including hyperglycemia. To shed light on the effect and action mechanism of dexamethasone, we examined the alterations of gene expression levels caused by dexamethasone. Microarray analysis was performed on whole blood collected from 24 physically healthy subjects at baseline and after dexamethasone administration. The expression levels of resistin mRNA were found to be significantly increased after the dexamethasone administration. In a separate sample of 12 subjects, we examined plasma resistin protein levels and found that they were increased after dexamethasone administration. Furthermore, the plasma mRNA and protein levels of resistin were significantly higher in individuals who carried the A allele of RETN single nucleotide polymorphism rs3219175 than in those who did not carry the allele. There was no significant interaction between the genotype and dexamethasone administration. No significant correlation was found between plasma levels of cortisol and resistin. Consistent with previous studies, the genotype of RETN rs3219175 was a strong determinant of resistin levels. The present study showed that oral administration of dexamethasone increases the protein and mRNA levels of resistin irrespective of the rs3219175 genotype.
Increased sensitivity to interferon-α in psoriatic T cells

Psoriasis is a chronic inflammatory skin disease characterized by abnormal epidermal proliferation. Several studies have shown that skin-infiltrating activated T cells and cytokines play a pivotal role during the initiation and maintenance of the disease. Interferon (IFN)-α plays an important role in host defense against infections, but recent data have also implicated IFN-α in psoriasis. Thus, IFN-α induces or aggravates psoriasis in some patients, and mice lacking a transcriptional attenuator of IFN-α/β signaling spontaneously develop a psoriasis-like inflammatory skin disease characterized by CD8 + -infiltrating T cells. In this study, we therefore investigate IFN-α signaling in T cells isolated from involved skin of psoriatic patients. We show that psoriatic T cells have increased and prolonged responses to IFN-α, on the level of signal transducers and activators of transcription (STAT) activation, compared with infiltrating T cells from skin of non-psoriatic donors. Functionally, the increased IFN-α signaling leads to an increased binding of STAT4 to the IFN-γ promotor, IFN-γ production, and inhibition of T cell growth. In contrast, to STAT responses to other cytokines were not changed in psoriasis. In conclusion, we provide evidence that psoriatic T cells have an increased sensitivity to IFN-α. Thus, our data suggest that increased IFN-α signaling is involved in the pathogenesis of psoriasis.
Role of laparoscopy in nonspecific abdominal pain

Objective: The aim was to determine the role of laparoscopy in the management of nonspecific abdominal pain (NSAP). Background: NSAP constitutes a good proportion of surgical admissions, both in emergency and elective settings with considerable diagnostic dilemma. Patients and Methods: All patients who presented with pain abdomen with no immediate cause and were labeled as NSAP after clinical assessment and investigations and following that underwent laparoscopy to make a definitive diagnosis were included in the study. Results: A total of 88 patients were included in the study. There were 59 (67%) females and 29 (33%) males. The mean age was 26 years (range 18-58 year). The common mode of admission was out-patient department 69 (78.4%) patients. Twenty-five (28.4%) patients presented with NSAP in lower abdomen, followed by 21 (23.8%) with right lower abdominal pain and 19 (21.5%) with central pain radiating to right lower abdomen. Diagnosis was established in 75 (85.2%) patients. In 13 (14.7%) no pathology was found. The most common diagnosis was pathology of appendix in 29 (32.9%) patients followed by pelvic pathology in 18 (20.4%) and abdominal tuberculosis in 14 (15.9%) patients. Most 37 (42%) of the patients stayed in the hospital for 24 h. There was no readmission and no major postoperative complications. Conclusions: Laparoscopy has a definitive role in diagnostic dilemma associated with NSAP. It has at the same time role in treatment of the condition; hence laparoscopy has a diagnostic and a therapeutic implication in management of NSAP.
Alcohol and Other Drug Use among College Students: Intimate Partner Violence and Health-Compromising Behaviors

ABSTRACT A survey was conducted with 400 male and female college students to examine the relationships among intimate partner violence (IPV), alcohol and other drug use, and health-compromising behaviors. The results indicated that IPV was directly related to alcohol use. The results further indicated that alcohol and drug use are directly and IPV is indirectly associated with risky sexual behaviors. Victimized male and female students had higher rates of alcohol and drug use and other health-compromising behaviors compared to nonvictimized male and female students. Implications are suggested for collegiate prevention programs.