Miracle-dz/UniFAR-FTUs huggingface dataset:
Intravital videomicroscopic evidence for regulation of metastasis by the hepatic microvasculature: effects of interleukin-1alpha on metastasis and the location of B16F1 melanoma cell arrest.

There have been few reported visual observations of metastatic cancer cell arrest in vivo. To seek evidence that inducible vascular adhesive properties can regulate hepatic metastasis, groups of 9-14 c57bl/6 mice were given 1.5 microg of interleukin-1alpha (IL-1alpha) 4 h before the injection of 3 x 10(5) B16F1 melanoma cells into a mesenteric vein. After 7 days, these mice had an 11-22-fold greater hepatic tumor burden than controls given i.p. saline. In both groups, small metastases were seen in the portal tract region. Twice as many 125I-labeled UdR-labeled B16F1 cells were detected in the livers of IL-1alpha-treated animals 5 min after injection, and 7 times as many were found after 24 h. Intravital videomicroscopy showed marked differences in the arrest pattern of the B16F1 cells between controls and IL-1alpha-treated mice. In controls, arrest occurred at a median distance of 32 microm beyond the sinusoidal inlet, where the median sinusoidal diameter was 16 microm. However, in IL-1alpha-treated mice, arrest occurred in the presinusoidal portal vein branches, which had a median diameter of 34 microm. Maximum observed tumor cell velocities were 2-fold less in the IL-1alpha-treated mice, although there was no significant difference in the flow rate of RBCs. To look for effects on the adhesive properties of the hepatic microvasculature, 5 x 10(4) B16F1 cells were incubated for 15 min on 5-microm sections of liver from control and IL-1alpha-treated mice. Three-fold more cells adhered to sections of liver from IL-1alpha-treated mice. This phenomenon was blocked by GRGDS peptides and by antibodies to E-selectin, ICAM-1, VCAM-1, and the alpha v integrin subunit. We postulate that pretreatment of mice with IL-1alpha alters a number of adhesive interactions between B16F1 cells and the hepatic microvasculature, contributing to the site of arrest and to the subsequent fate of the arrested cells.
Temperature dependence of active tension in mammalian (rabbit psoas) muscle fibres: effect of inorganic phosphate

1 The effect of added inorganic phosphate (Pi, range 3–25 mm) on active tension was examined at a range of temperatures (5–30 °C) in chemically skinned (0.5 % Brij) rabbit psoas muscle fibres. Three types of experiments were carried out. 2 In one type of experiment, a muscle fibre was maximally activated at low temperature (5 °C) and its tension change was recorded during stepwise heating to high temperature in ≈60 s. As found in previous studies, the tension increased with temperature and the normalised tension‐(reciprocal) temperature relation was sigmoidal, with a half‐maximal tension at 8 °C. In the presence of 25 mm added Pi, the temperature for half‐maximal tension of the normalised curve was ≈5 °C higher than in the control. The difference in the slope was small. 3 In a second type of experiment, the tension increment during a large temperature jump (from 5 to 30 °C) was examined during an active contraction. The relative increase of active tension on heating was significantly higher in the presence of 25 mm added Pi (30/5 °C tension ratio of 6–7) than in the control with no added Pi (tension ratio of ≈3). 4 In a third type of experiment, the effect on the maximal Ca2+‐activated tension of different levels of added Pi (3–25 mm) (and Pi mop adequate to reduce contaminating Pi to micromolar levels) was examined at 5, 10, 20 and 30 °C. The tension was depressed with increased [Pi] in a concentration‐dependent manner at all temperatures, and the data could be fitted with a hyperbolic relation. The calculated maximal tension depression in excess [Pi] was ≈65 % of the control at 5–10 °C, in contrast to a maximal depression of 40 % at 20 °C and 30 % at 30 °C. 5 These experiments indicate that the active tension depression induced by Pi in psoas fibres is temperature sensitive, the depression becoming less marked at high temperatures. A reduced Pi‐induced tension depression is qualitatively predicted by a simplified actomyosin ATPase cycle where a pre‐phosphate release, force‐generation step is enhanced by temperature.
Two-hole plate fixation for traumatic symphysis pubis diastasis.

Techniques for managing traumatic diastasis of the pubic symphysis include bed rest, hip spica casting, pelvic slings, external fixation, and internal fixation. We report herein our experience with 14 consecutively managed patients in whom we successfully stabilized traumatic pubic diastasis with a single two-hole plate fixation. The average age of the 13 men and one woman was 30 years; followup averaged 17 months. Most of the patients had associated injuries (Injury Severity Score average, 19). Nine patients had concomitant disruption of the sacroiliac joint requiring either delayed open reduction and internal fixation or prolonged skeletal traction; among the five remaining patients, time to mobilization (bed to chair) averaged 1 day. There were no complications attributable to the procedure; i.e., no infections, and no failures of fixation. In this small series of patients early two-hole plate fixation of the traumatic diastasis of the pubis satisfactorily restored the disrupted anterior pelvic ring, contributed to early mobilization of the patients, and made reduction of a concomitantly disrupted sacroiliac joint easier, whether accomplished by skeletal traction or open reduction and internal fixation during a second procedure.
Benthic communities under anthropogenic pressure show resilience across the Quaternary

The Southeast Pacific is characterized by rich upwelling systems that have sustained and been impacted by human groups for at least 12 ka. Recent fishing and aquaculture practices have put a strain on productive coastal ecosystems from Tongoy Bay, in north-central Chile. We use a temporal baseline to determine whether potential changes to community structure and composition over time are due to anthropogenic factors, natural climatic variations or both. We compiled a database (n = 33 194) with mollusc species abundances from the Mid-Pleistocene, Late Pleistocene, Holocene, dead shell assemblages and live-sampled communities. Species richness was not significantly different, neither were diversity and evenness indices nor rank abundance distributions. There is, however, an increase in relative abundance for the cultured scallop Argopecten, while the previously dominant clam Mulinia is locally very rare. Results suggest that impacts from both natural and anthropogenic stressors need to be better understood if benthic resources are to be preserved. These findings provide the first Pleistocene temporal baseline for the south Pacific that shows that this highly productive system has had the ability to recover from past alterations, suggesting that if monitoring and management practices continue to be implemented, moderately exploited communities from today have hopes for recovery.
EphA Receptors Direct the Differentiation of Mammalian Neural Precursor Cells through a Mitogen-activated Protein Kinase-dependent Pathway*

Ephrins are cell surface-associated ligands for Eph receptor tyrosine kinases and are implicated in repulsive axon guidance and cell migration. EphA2, 3, and 4 receptors and one of their cognate ligands, ephrin-A2, are expressed by cells in the subventricular zone and ganglionic eminence of the embryonic day 14.5 telencephalon and by neural precursor cells in vitro. Activation of EphA receptors in dissociated neural precursor cells in vitro facilitates the commitment to neuronal fates. The majority of ephrin-A1-induced neurons is immunoreactive for tyrosine hydroxylase. Blocking the signal by the extracellular domain of EphA in forebrain slices results in a decrease in neurogenesis. Extracellular signal-regulated kinase is activated by the ligand binding to EphA receptors and is involved in the neurogenesis through EphA receptors. Rap1, but not Ras, is activated in response to ephrin-A1. Our results identify EphA receptors as positive regulators of the mitogen-activated protein kinase pathway that exerts neurogenesis of neural precursor cells from the developing central nervous system.
Changes in the foraging dive behaviour and energetics of king penguins through summer and autumn: a month by month analysis

King penguins Aptenodytes patagonicus are known to change their diving behaviour in response to changes in both prey location and their breeding status through the early stages of the breeding cycle (austral summer and autumn). However, little information exists on whether and how these changes affect the energy expenditure of such behaviour. By deploying heart rate and hydro- static pressure data loggers, we investigated detailed changes in the dive time budgeting of king penguins during foraging dives across the breeding season, in the same individuals, and the associ- ated changes in estimated oxygen consumption during those dives. Maximum dive depth, duration, bottom duration, feeding events (indicated by wiggles) per dive and post-dive duration increased through the study period. While a foraging dive later in the breeding season was energetically more costly than a dive earlier in the season, the overall rate of energy expenditure did not change, nor did energy cost per unit prey capture. These findings indicate an ability of king penguins to adjust their foraging dive behaviours through the summer and autumn without affecting the energetic costs of diving to capture prey. Such plasticity may be necessary to compensate for changes both in prey loca- tion and abundance, and in the energy requirements of the chick over time.
Is Acupuncture Analgesia an Expectancy Effect?

This purpose of this article is to contrast the analgesic efficacy of acupuncture following dental surgery with the analgesic effects based on the expectation of benefit in two independently conducted placebo-controlled trials evaluating acupuncture as an adjunctive therapy for dental surgery. Both trials used pain following dental surgery as the outcome variable, and both included a blinding check to ascertain patients’ beliefs regarding which treatment they were receiving. Although no statistically significant analgesic effect was observed between the acupuncture and placebo groups, participants in both experiments who believed they received real acupuncture reported significantly less pain than patients who believed that they received a placebo. Patients’ beliefs regarding the receipt of acupuncture bore a stronger relationship to pain than any specific action possessed by acupuncture. These results also support the importance of both employing credible controls for the placebo effect in clinical trials and evaluating the credibility of those controls.
AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins

Recent studies show that Hox homeodomain proteins from paralog groups 1 to 10 gain DNA binding specificity and affinity through cooperative binding with the divergent homeodomain protein Pbx1. However, the AbdB-like Hox proteins from paralogs 11, 12, and 13 do not interact with Pbx1a, raising the possibility of different protein partners. The Meis1 homeobox gene has 44% identity to Pbx within the homeodomain and was identified as a common site of viral integration in myeloid leukemias arising in BXH-2 mice. These integrations result in constitutive activation of Meis1. Furthermore, the Hoxa-9 gene is frequently activated by viral integration in the same BXH-2 leukemias, suggesting a biological synergy between these two distinct classes of homeodomain proteins in causing malignant transformation. We now show that the Hoxa-9 protein physically interacts with Meis1 proteins by forming heterodimeric binding complexes on a DNA target containing a Meis1 site (TGACAG) and an AbdB-like Hox site (TTTTACGAC). Hox proteins from the other AbdB-like paralogs, Hoxa-10, Hoxa-11, Hoxd-12, and Hoxb-13, also form DNA binding complexes with Meis1b, while Hox proteins from other paralogs do not appear to interact with Meis1 proteins. DNA binding complexes formed by Meis1 with Hox proteins dissociate much more slowly than DNA complexes with Meis1 alone, suggesting that Hox proteins stabilize the interactions of Meis1 proteins with their DNA targets.
An Attempt to Integrate Previous Localized Estimates of Human Inbreeding for the Whole of Britain

ABSTRACT There have been a number of previous estimates of human inbreeding for Britons of British descent in Britain, each generally for different social classes, geographical regions, and/or time periods. In this study I attempted to collect all relevant published studies and combine these disparate results into an integrated whole for all of Britain. This was achieved by combining weighted means of the percentage of consanguineous marriages (f%) reported in these earlier studies: weighted according to the number of records each author examined, the proportion of social classes or geographic regions covered by the records, and the “merit” of their individual research methodologies. The percentage occurrences of the various consanguineous marriages, from first to third cousins, were partitioned into a number of time periods, which allowed the weighted mean percentage inbreeding coefficients (F%) to be obtained as a function of time over the period from 1820 to 1960. The resulting temporal scatter distribution of the weighted F% values closely followed a sigmoidal curve, with a nonlinear correlation coefficient of η = 0.974, which fitted well to a generalized logistic function. After about 1900 the value of the weighted F% was essentially constant at about 0.038 ± 0.004, whereas it decreased rapidly from about 0.256 ± 0.011 between 1820 and 1900. The upper-bound value of weighted F% before 1820 from the fitted logistic function is 0.276. This corresponds to a value of the conventional mean inbreeding coefficient F = 0.00276. As the first known attempt to integrate the earlier disparate values of unweighted F% for Britons of British descent for all of Britain, the results of this analysis are promising and should be useful as reference values in other related studies.
Immunolocalization of Dinitrogenase Reductase Produced by Klebsiella pneumoniae in Association withZea mays L

ABSTRACT The endophytic lifestyle of Klebsiella pneumoniae is described, including the production of dinitrogenase reductase by bacteria residing in maize root tissue. The green fluorescent protein (GFP) was used to detect the colonization of maize by K. pneumoniae strains 2028 and 342. These strains were found to reside in intercortical layers of the stem and within the region of maturation in the root. The production of dinitrogenase reductase by GFP-tagged bacteria was visualized using immunolocalization. This activity was only apparent when bacteria were supplied with an exogenous carbon source. The results suggest that maize provides a suitable habitat for K. pneumoniae and that this species is capable of producing nitrogenase under the appropriate plant cultivation conditions.
Molecular Epidemiology of Rotavirus Diarrhea among Children and Adults in Nepal: Detection of G12 Strains with P[6] or P[8] and a G11P[25] Strain

ABSTRACT In anticipation of a rotavirus vaccine in Nepal, this study was undertaken to determine the distribution of the G and P serotypes and electropherotypes of rotaviruses in order to examine if there is any emerging serotype or unusual strain circulating in children and adults in Nepal. Of 1,315 diarrheal stool specimens, rotavirus was detected by an enzyme-linked immunosorbent assay in 116 (17%) of 666 patients less than 5 years of age, in 18 (7%) of 260 patients 5 to 14 years of age, and in 19 (5%) of 358 patients 15 years of age and older. Approximately 75% of rotavirus diarrhea occurred in children less than 5 years of age. Approximately 70% of rotaviruses found in each of the three age groups belonged to serotype G1P[8]. Interestingly, there were 29 (20%) G12 rotaviruses carrying either P[8] or P[6] and one (0.7%) G11 rotavirus carrying an unusual P[25] genotype. RNA polyacrylamide gel electrophoresis discriminated 19 strains (electropherotypes), among which there were three codominant strains carrying G1P[8] and long RNA patterns. Five electropherotypes were discriminated among G12 rotaviruses, all of which had long RNA patterns. The fact that 20% of rotaviruses were G12 strains carrying either P[8] or P[6] and had multiple electropherotypes suggest that G12 strains are not more rare strains but that they pose an emerging challenge to current and future vaccines. The presence of multiple strains as defined by electropherotypes suggests the richness of the rotavirus gene pool in Nepal, where unusual strains may continue to emerge.
Growth of Thermophilic and Hyperthermophilic Fe(III)-Reducing Microorganisms on a Ferruginous Smectite as the Sole Electron Acceptor

ABSTRACT Recent studies have suggested that the structural Fe(III) within phyllosilicate minerals, including smectite and illite, is an important electron acceptor for Fe(III)-reducing microorganisms in sedimentary environments at moderate temperatures. The reduction of structural Fe(III) by thermophiles, however, has not previously been described. A wide range of thermophilic and hyperthermophilic Archaea and Bacteria from marine and freshwater environments that are known to reduce poorly crystalline Fe(III) oxides were tested for their ability to reduce structural (octahedrally coordinated) Fe(III) in smectite (SWa-1) as the sole electron acceptor. Two out of the 10 organisms tested, Geoglobus ahangari and Geothermobacterium ferrireducens, were not able to conserve energy to support growth by reduction of Fe(III) in SWa-1 despite the fact that both organisms were originally isolated with solid-phase Fe(III) as the electron acceptor. The other organisms tested were able to grow on SWa-1 and reduced 6.3 to 15.1% of the Fe(III). This is 20 to 50% less than the reported amounts of Fe(III) reduced in the same smectite (SWa-1) by mesophilic Fe(III) reducers. Two organisms, Geothermobacter ehrlichii and archaeal strain 140, produced copious amounts of an exopolysaccharide material, which may have played an active role in the dissolution of the structural iron in SWa-1 smectite. The reduction of structural Fe(III) in SWa-1 by archaeal strain 140 was studied in detail. Microbial Fe(III) reduction was accompanied by an increase in interlayer and octahedral charges and some incorporation of potassium and magnesium into the smectite structure. However, these changes in the major element chemistry of SWa-1 smectite did not result in the formation of an illite-like structure, as reported for a mesophilic Fe(III) reducer. These results suggest that thermophilic Fe(III)-reducing organisms differ in their ability to reduce and solubilize structural Fe(III) in SWa-1 smectite and that SWa-1 is not easily transformed to illite by these organisms.
Conducting cognitive interviews to understand question-response limitations.

OBJECTIVES To illustrate how cognitive interviewing techniques can be used to identify question-response problems for poorer and less educated survey respondents and to describe how qualitative analyses of interviews can improve survey questionnaires. METHODS A cognitive interviewing project testing a general health questionnaire was administered in southern rural Mississippi. RESULTS Three themes of response difficulty were identified: 1) responding within a survey interaction, 2) making mathematical calculations, and 3) responding within another system of knowledge. CONCLUSION Understanding how sociocultural factors impact the response process is vital for survey research.
Patient and Family Satisfaction with Discharge Plans

This study explored factors affecting patient and family satisfaction with discharge plans, an outcome of discharge planning provided by the hospital department of social work. Adequacy of discharge plans was rated by 126 patients and 130 family members within 24 hours before hospital discharge. Patient ratings of discharge plans were related to their degree of involvement in decisionmaking, social support networks (sex and marital status), and physical condition (diagnosis and functional ability). Three types of factors were important to family members: 1) factors related to the discharge planning process, 2) the patient's discharge destination, and 3) length of patient hospital stay. Findings are discussed in terms of their implications on maximizing consumer satisfaction in the cost-conscious environment.
Selective noradrenaline reuptake inhibitor atomoxetine directly blocks hERG currents

Background and purpose: Atomoxetine is a selective noradrenaline reuptake inhibitor, recently approved for the treatment of attention‐deficit/hyperactivity disorder. So far, atomoxetine has been shown to be well tolerated, and cardiovascular effects were found to be negligible. However, two independent cases of QT interval prolongation, associated with atomoxetine overdose, have been reported recently. We therefore analysed acute and subacute effects of atomoxetine on cloned human Ether‐à‐Go‐Go‐Related Gene (hERG) channels.
Convergence of Cahn-Hilliard systems to the Stefan problem with dynamic boundary conditions

This paper examines the well-posedness of the Stefan problem with a dynamic boundary condition. To show the existence of the weak solution, the original problem is approximated by a limit of an equation and dynamic boundary condition of Cahn-Hilliard type. By using this Cahn-Hilliard approach, it becomes clear that the state of the mushy region of the Stefan problem is characterized by an asymptotic limit of the fourth-order system, which has a double-well structure. This fact also raises the possibility of the numerical application of the Cahn-Hilliard system to the degenerate parabolic equation, of which the Stefan problem is one.
Analysis of Novel Brushless DC Motors Made of Soft Magnetic Composite Core

The soft magnetic composite material has merits of the low eddy current loss and the flexible machine design and assembly. This paper develops new brushless DC motors made of this material. The shape of the developed motor is similar to a hybrid type stepping motor, which utilizes three-dimensional isotropic ferromagnetic behavior, and the motor has hollows in the stator main teeth in order to reduce the copper loss and the motor size. This paper clarifies the steady state characteristics of the motors using the three-dimensional finite element analysis. The motor efficiency becomes larger than that of the conventional laminated core, when the rotating speed is high, or if the motor size becomes large.
PREDICTION OF BODY COMPOSITION OF LIVE AND POST-MORTEM RED FOXES

A series of measurements (lengths, circumferences, skinfolds, masses and resistance) was taken on 29 red foxes (Vulpes vulpes) of both sexes before and after their death during the autumns of 1994 and 1995. Body composition of each carcass was determined by chemical analysis of homogenized samples of viscera, carcass and skin. Eight multiple regression models were then developed to predict body water, fat, protein, and mineral mass using body measurements as independent variables taken on live or dead animals. All final models were highly significant (P < 0.0001) and included three or four explanatory variables. Adjusted coefficients of determination varied between 0.95 for water mass and 0.81 for mineral mass. The models cover a wide range of conditions as percent body fat in the 29 samples varied between 1.1 and 28.4%. Our models can serve for management or research purposes with live or dead red foxes as they are quick, inexpensive and nondestructive.