Miracle-dz/UniFAR-FTUs huggingface dataset:
Poor Parental Sleep and the Reported Sleep Quality of Their Children

BACKGROUND: Pediatric sleep disturbances are regularly diagnosed on the basis of parental reports. However, the impact of parental sleeping problems on parental perceptions and reports of their child’s sleep has not yet been studied. We hypothesized that poor parental sleep decreases the parent-reported child sleep quality. METHODS: A 1-week actigraph recording was performed in 100 children aged 2 to 6 years recruited in 16 day care centers. Their biological parents completed a sleep diary and a Sleep Disturbance Scale for Children (SDSC) on children’s sleep. The parents also completed the Jenkins’ sleep scale on their own sleep, the 12-item General Health Questionnaire, and questions on demographic factors. Linear regression analyses were performed to study the association of the parental Jenkins’ score on their child’s total SDSC score. Analyses were also performed for 3 of the subscales of the SDSC: disorders of excessive somnolence, disorders of initiating and maintaining sleep, and sleep-wake transition disorders. RESULTS: Parental sleeping problems were associated with more frequent reporting of children’s sleeping problems. This association was unexplained by the actigraph measures of children’s sleep, such as actual 24-hour sleep time and sleep efficiency, parental mental health problems, or any other tested potential confounder or mediator. Similar correlations were seen for the 3 analyzed subscales. CONCLUSIONS: Parental sleep quality was associated with overreporting of sleep problems in their children. This finding emphasizes the importance of considering parental sleep quality in the diagnosis, treatment, and research of pediatric sleeping problems.
Provision of Preventive Dental Services in Children Enrolled in Medicaid by Nondental Providers

OBJECTIVES: Aims of this study are to determine (1) the association of oral health services (OHS) provided by nontraditional providers with the percentage of Medicaid children 0 to 5 years of age who receive ≥1 preventive services from all provider types in the United States; and (2) characteristics of state Medicaid policies associated with provision of OHS. METHODS: We conducted a time-series cross-sectional study of preventive services provided by nontraditional (OHS) and dental (PDS) providers for Medicaid-enrolled children from birth to 5 years of age in all states during 2010 to 2013 (204 observations). We applied panel data multiple regression analysis techniques to exploit year and state variation in aggregate data available in Centers for Medicare and Medicaid Services reports (form CMS-416). Total preventive dental services (TPDS =OHS + PDS) was predicted by months since state enactment of a policy to reimburse medical providers for OHS. RESULTS: The 44 states with a policy reported 4.3% of children per state per year with any OHS. For all states, an average of 30.1% received PDS and 34.5% TPDS. The delivery of OHS was associated with a small increase in percentage with TPDS. One year of Medicaid OHS availability was associated with an increase of 1.5% in the percentage of children with TPDS per state per year. CONCLUSIONS: Implementation of policies by Medicaid programs to support integration of OHS into primary care is associated with increases in overallTPDS use, but efforts are needed to improve implementation in practice to achieve national impact on access.
Molecular mechanisms of kinesin-14 motors in spindle assembly and chromosome segregation

ABSTRACT During eukaryote cell division, molecular motors are crucial regulators of microtubule organization, spindle assembly, chromosome segregation and intracellular transport. The kinesin-14 motors are evolutionarily conserved minus-end-directed kinesin motors that occur in diverse organisms from simple yeasts to higher eukaryotes. Members of the kinesin-14 motor family can bind to, crosslink or slide microtubules and, thus, regulate microtubule organization and spindle assembly. In this Commentary, we present the common subthemes that have emerged from studies of the molecular kinetics and mechanics of kinesin-14 motors, particularly with regard to their non-processive movement, their ability to crosslink microtubules and interact with the minus- and plus-ends of microtubules, and with microtubule-organizing center proteins. In particular, counteracting forces between minus-end-directed kinesin-14 and plus-end-directed kinesin-5 motors have recently been implicated in the regulation of microtubule nucleation. We also discuss recent progress in our current understanding of the multiple and fundamental functions that kinesin-14 motors family members have in important aspects of cell division, including the spindle pole, spindle organization and chromosome segregation. Summary: During cell division, kinesin-14 motors are conserved regulators of spindle assembly and chromosome segregation. We provide a detailed view of their cellular functions in diverse organisms.
Restful web services vs. "big"' web services: making the right architectural decision

Recent technology trends in the Web Services (WS) domain indicate that a solution eliminating the presumed complexity of the WS-* standards may be in sight: advocates of REpresentational State Transfer (REST) have come to believe that their ideas explaining why the World Wide Web works are just as applicable to solve enterprise application integration problems and to simplify the plumbing required to build service-oriented architectures. In this paper we objectify the WS-* vs. REST debate by giving a quantitative technical comparison based on architectural principles and decisions. We show that the two approaches differ in the number of architectural decisions that must be made and in the number of available alternatives. This discrepancy between freedom-from-choice and freedom-of-choice explains the complexity difference perceived. However, we also show that there are significant differences in the consequences of certain decisions in terms of resulting development and maintenance costs. Our comparison helps technical decision makers to assess the two integration styles and technologies more objectively and select the one that best fits their needs: REST is well suited for basic, ad hoc integration scenarios, WS-* is more flexible and addresses advanced quality of service requirements commonly occurring in enterprise computing.
Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm of mouse embryos

ABSTRACT The small GTPase Arl8b localizes primarily to lysosomes and is involved in lysosomal motility and fusion. Here, we show that Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm (VYSE), an apical cell layer of the visceral yolk sac, of mouse embryos. The VYSE actively takes up maternal materials from uterine fluid and degrades them in lysosomes to provide breakdown products to the embryo. Arl8b gene-trap mice (Arl8b−/−) displayed decreased early embryo body size. The Arl8b−/− VYSE exhibited defective endocytic trafficking to the lysosome and accumulation of maternal proteins such as albumin and immunoglobulin G in late endocytic organelles. Furthermore, Transthyretin-Cre;Arl8bflox/flox mice in which Arl8b was ablated specifically in the VYSE also showed decreased embryo body size, defects in trafficking to the lysosome and reduction of the free amino acid level in the embryos. Taken together, these results suggest that Arl8b mediates lysosomal degradation of maternal proteins in the VYSE, thereby contributing to mouse embryonic development. Summary: The small GTPase Arl8b, known to be involved in lysosomal function, contributes to mouse embryonic growth by mediating lysosomal degradation of maternal proteins in the visceral yolk sac endoderm.
The DUX4 homeodomains mediate inhibition of myogenesis and are functionally exchangeable with the Pax7 homeodomain

ABSTRACT Facioscapulohumeral muscular dystrophy (FSHD) is caused by inappropriate expression of the double homeodomain protein DUX4. DUX4 has bimodal effects, inhibiting myogenic differentiation and blocking MyoD at low levels of expression, and killing myoblasts at high levels. Pax3 and Pax7, which contain related homeodomains, antagonize the cell death phenotype of DUX4 in C2C12 cells, suggesting some type of competitive interaction. Here, we show that the effects of DUX4 on differentiation and MyoD expression require the homeodomains but do not require the C-terminal activation domain of DUX4. We tested the set of equally related homeodomain proteins (Pax6, Pitx2c, OTX1, Rax, Hesx1, MIXL1 and Tbx1) and found that only Pax3 and Pax7 display phenotypic competition. Domain analysis on Pax3 revealed that the Pax3 homeodomain is necessary for phenotypic competition, but is not sufficient, as competition also requires the paired and transcriptional activation domains of Pax3. Remarkably, substitution mutants in which DUX4 homeodomains are replaced by Pax7 homeodomains retain the ability to inhibit differentiation and to induce cytotoxicity. Highlighted Article: The facioscapulohumeral muscular dystrophy-associated protein DUX4 inhibits myogenic differentiation via its homeodomains, which bear similarity to, and can be exchanged with, those of the muscle stem cell master regulator, Pax7.
Distinct intracellular sAC-cAMP domains regulate ER Ca2+ signaling and OXPHOS function

ABSTRACT cAMP regulates a wide variety of physiological functions in mammals. This single second messenger can regulate multiple, seemingly disparate functions within independently regulated cell compartments. We have previously identified one such compartment inside the matrix of the mitochondria, where soluble adenylyl cyclase (sAC) regulates oxidative phosphorylation (OXPHOS). We now show that sAC knockout fibroblasts have a defect in OXPHOS activity and attempt to compensate for this defect by increasing OXPHOS proteins. Importantly, sAC knockout cells also exhibit decreased probability of endoplasmic reticulum (ER) Ca2+ release associated with diminished phosphorylation of the inositol 3-phosphate receptor. Restoring sAC expression exclusively in the mitochondrial matrix rescues OXPHOS activity and reduces mitochondrial biogenesis, indicating that these phenotypes are regulated by intramitochondrial sAC. In contrast, Ca2+ release from the ER is only rescued when sAC expression is restored throughout the cell. Thus, we show that functionally distinct, sAC-defined, intracellular cAMP signaling domains regulate metabolism and Ca2+ signaling. Highlighted Article: Soluble adenylyl cyclase (sAC) regulates Ca2+ release from the ER. Although sAC domains in ER and mitochondria are distinct, Ca2+ release from the ER provides a functional link between the two organelles.
The HECT E3 ubiquitin ligase NEDD4 interacts with and ubiquitylates SQSTM1 for inclusion body autophagy

ABSTRACT Our previous studies have shown that the HECT E3 ubiquitin ligase NEDD4 interacts with LC3 and is required for starvation and rapamycin-induced activation of autophagy. Here, we report that NEDD4 directly binds to SQSTM1 via its HECT domain and polyubiquitylates SQSTM1. This ubiquitylation is through K63 conjugation and is not involved in proteasomal degradation. Mutational analysis indicates that NEDD4 interacts with and ubiquitylates the PB1 domain of SQSTM1. Depletion of NEDD4 or overexpression of the ligase-defective mutant of NEDD4 induced accumulation of aberrant enlarged SQSTM1-positive inclusion bodies that are co-localized with the endoplasmic reticulum (ER) marker CANX, suggesting that the ubiquitylation functions in the SQSTM1-mediated biogenic process in inclusion body autophagosomes. Taken together, our studies show that NEDD4 is an autophagic E3 ubiquitin ligase that ubiquitylates SQSTM1, facilitating SQSTM1-mediated inclusion body autophagy. Highlighted Article: NEDD4 is an autophagic HECT E3 ubiquitin ligase that ubiquitylates the autophagy receptor SQSTM1, facilitating inclusion body autophagy.
Hematological and immunological effect of Cannabis sativa on albino Wistar rats.

The present study is aimed at ascertaining the effect of oral feeding of Cannabis sativa on blood parameters and immunity in rats. Administration of Cannabis sativa was done by orogastric feeding. All the groups had free access to water and food. The experiment lasted for 28 days. At the end of the 28 days of feeding, we observed that the mean red blood cell count was 5.68±0.09 x 10 6 /mm 3 , 5.90± 0.04 x 10 6 mm 3 and 6 . 38 ±0.22 x 10 6 mm 3 for control, low dose (LD) and high dose (HD) respectively. The red blood cells count in high dose was significantly higher (P<0.05) than low dose and control (P<0.01) respectively. However there was no significant difference between c ontrol and low dose. The total white blood cell count for control, LD and HD groups was 2.26±0.13 x 10 3 /mm 3 , 1.69± 0.04 x 10 3 mm 3
Copper and zinc levels in patients with cervical neoplasia: Clinical evaluation of the copper/zinc ratio

SummaryPlasma levels of copper and zinc were determined by flame atomic absorption spectrometry in 25 patients with invasive carcinoma of the cervix, 16 patients with cervical dysplasia, and 19 controls who were attending the gynaecological clinic. The plasma copper levels in the 25 patients with cervical cancer did not differ from the controls, but the plasma zinc levels were lower than in the control group. Measurements of plasma copper and zinc in the cervical dysplasia patients did not differ from those in the controls.Although the plasma zinc and plasma copper/zinc ratio between the cancer patients differed from those in the other two groups of patients the differences are not enough to serve as markers for invasive cervical lesion.
Neonatal Jaundice: Improved Quality and Cost Savings After Implementation of a Standard Pathway

OBJECTIVES: Seattle Children’s Hospital sought to optimize the value equation for neonatal jaundice patients by creating a standard care pathway. METHODS: An evidence-based pathway for management of neonatal jaundice was created. This included multidisciplinary team assembly, comprehensive literature review, creation of a treatment algorithm and computer order sets, formulation of goals and metrics, roll-out of an education program for end users, and ongoing pathway improvement. The pathway was implemented on May 31, 2012. Quality metrics before and after implementation were compared. External data were used to analyze cost impacts. RESULTS: Significant improvements were achieved across multiple quality dimensions. Time to recovery decreased: mean length of stay was 1.30 days for 117 prepathway patients compared with 0.87 days for 69 postpathway patients (P < .001). Efficiency was enhanced: mean time to phototherapy initiation was 101.26 minutes for 14 prepathway patients compared with 54.67 minutes for 67 postpathway patients (P = .03). Care was less invasive: intravenous fluid orders were reduced from 80% to 44% (P < .001). Inpatient use was reduced: 66% of prepathway patients were admitted from the emergency department to inpatient care, compared with 50% of postpathway patients (P = .01). There was no increase in the readmission rate. These achievements translated to statistically significant cost reductions in total charges, as well as in the following categories: intravenous fluids, laboratory, room cost, and emergency department charges. CONCLUSIONS: An evidence-based standard care pathway for neonatal jaundice can significantly improve multiple dimensions of value, including reductions in cost and length of stay.
Language Outcomes at 7 Years: Early Predictors and Co-Occurring Difficulties

The ability of early factors to predict low language abilities at 7 years is explored together with the degree and nature of co-occurring difficulties present. OBJECTIVE: To examine at 7 years the language abilities of children, the salience of early life factors and language scores as predictors of language outcome, and co-occurring difficulties METHODS: A longitudinal cohort study of 1910 infants recruited at age 8 to 10 months. Exposures included early life factors (sex, prematurity, birth weight/order, twin birth, socioeconomic status, non–English speaking background,family history of speech/language difficulties); maternal factors (mental health, vocabulary, education, and age); and child language ability at 2 and 4 years. Outcomes were 7-year standardized receptive or expressive language scores (low language: ≥1.25 SD below the mean), and co-occurring difficulties (autism, literacy, social, emotional, and behavioral adjustment, and health-related quality of life). RESULTS: Almost 19% of children (22/1204;18.9%) met criteria for low language at 7 years. Early life factors explained 9-13% of variation in language scores, increasing to 39-58% when child language scores at ages 2 and 4 were included. Early life factors moderately discriminated between children with and without low language (area under the curve: 0.68–0.72), strengthening to good discrimination with language scores at ages 2 and 4 (area under the curve: 0.85–0.94). Low language at age 7 was associated with concurrent difficulties in literacy, social-emotional and behavioral difficulties, and limitations in school and psychosocial functioning. CONCLUSIONS: Child language ability at 4 years more accurately predicted low language at 7 than a range of early child, family, and environmental factors. Low language at 7 years was associated with a higher prevalence of co-occurring difficulties.
Turkish pharmacy technicians' counseling practices and attitudes regarding emergency contraceptive pills

Supplying emergency contraception pills through community pharmacies without the need for a doctor’s prescription is an important public health role for the pharmacy staff, as a way of reducing unwanted pregnancies and induced abortions. Therefore, we aimed to assess the Turkish pharmacy technicians’ counseling practices and attitudes regarding emergency contraception pills. A survey tool which was structured to question pharmacy technicians’ demography, professional experience, counseling practices and attitudes regarding emergency contraception pills was administered to pharmacy technicians at the end of the “pharmacy technician certification program”. The fully completed questionnaires (n=145) were further analyzed. Mean [SEM] age of the pharmacy technicians was 25.3 [0.4] years and majority (89%) of them were practicing in a community pharmacy. Despite their positive attitude towards the emergency contraception pills, pharmacy technicians were found to provide insufficient emergency contraception pill counseling to clients. We suggest that organization of continuing education programs on “reproductive and sexual health and emergency contraception” for the pharmacy technicians could help increase the quality of emergency contraception pill counseling provided by the pharmacy technicians. Key words: Emergency contraception pill, counseling, attitude, community pharmacy, pharmacy technician.
Simulated annealing, its parameter settings and the longest common subsequence problem

Simulated Annealing is a probabilistic search heuristic for solving optimization problems and is used with great success on real life problems. In its standard form Simulated Annealing has two parameters, namely the initial temperature and the cooldown factor. In literature there are only rules of the thumb for choosing appropriate parameter values. This paper investigates the influence of different values for these two parameters on the optimization process from a theoretical point of view and presents some criteria for problem specific adjusting of these parameters. With these results the performance of the Simulated Annealing algorithm on solving the Longest Common Subsequence Problem is analysed using different values for the two parameters mentioned above. For all these parameter settings it is proved that even rather simple input instances of the Longest Common Subsequence Problem can neither be solved to optimality nor approximately up to an approximation factor arbitrarily close to 2 efficiently.
Digital Media and Sleep in Childhood and Adolescence

Given the pervasive use of screen-based media and the high prevalence of insufficient sleep among American youth and teenagers, this brief report summarizes the literature on electronic media and sleep and provides research recommendations. Recent systematic reviews of the literature reveal that the vast majority of studies find an adverse association between screen-based media consumption and sleep health, primarily via delayed bedtimes and reduced total sleep duration. The underlying mechanisms of these associations likely include the following: (1) time displacement (ie, time spent on screens replaces time spent sleeping and other activities); (2) psychological stimulation based on media content; and (3) the effects of light emitted from devices on circadian timing, sleep physiology, and alertness. Much of our current understanding of these processes, however, is limited by cross-sectional, observational, and self-reported data. Further experimental and observational research is needed to elucidate how the digital revolution is altering sleep and circadian rhythms across development (infancy to adulthood) as pathways to poor health, learning, and safety outcomes (eg, obesity, depression, risk-taking).
Shadow response in the blind cavefish Astyanax reveals conservation of a functional pineal eye

SUMMARY The blind cavefish Astyanax mexicanus undergoes bilateral eye degeneration during embryonic development. Despite the absence of light in the cave environment, cavefish have retained a structurally intact pineal eye. We show here that contrary to visual degeneration in the bilateral eyes, the cavefish pineal eye has conserved the ability to detect light. Larvae of two different Astyanax cavefish populations and the con-specific sighted surface-dwelling form (surface fish) respond similarly to light dimming by shading the pineal eye. As a response to shading, cavefish larvae swim upward vertically. This behavior resembles that of amphibian tadpoles rather than other teleost larvae, which react to shadows by swimming downward. The shadow response is highest at 1.5-days post-fertilization (d.p.f.), gradually diminishes, and is virtually undetectable by 7.5 d.p.f. The shadow response was substantially reduced after surgical removal of the pineal gland from surface fish or cavefish larvae, indicating that it is based on pineal function. In contrast, removal of one or both bilateral eye primordia did not affect the shadow response. Consistent with its light detecting capacity, immunocytochemical studies indicate that surface fish and cavefish pineal eyes express a rhodopsin-like antigen, which is undetectable in the degenerating bilateral eyes of cavefish larvae. We conclude that light detection by the pineal eye has been conserved in cavefish despite a million or more years of evolution in complete darkness.