Miracle-dz/UniFAR-FTUs huggingface dataset:
Methyl Jasmonate-Induced Changes of Flavor Profiles During the Processing of Green, Oolong, and Black Tea

Tea aroma is one of the most important factors affecting the character and quality of tea. Here we describe the practical application of methyl jasmonate (MeJA) to improve the aroma quality of teas. The changes of selected metabolites during crucial tea processing steps, namely, withering, fixing and rolling, and fermentation, were analyzed. MeJA treatment of tea leaves (12, 24, 48, and 168 h) greatly promotes the aroma quality of green, oolong, and black tea products when comparing with untreated ones (0 h) and as confirmed by sensory evaluation. MeJA modulates the aroma profiles before, during, and after processing. Benzyl alcohol, benzaldehyde, 2-phenylethyl alcohol, phenylacetaldehyde, and trans-2-hexenal increased 1.07- to 3-fold in MeJA-treated fresh leaves and the first two maintained at a higher level in black tea and the last two in green tea. This correlates with a decrease in aromatic amino acids by more than twofold indicating a direct relation to tryptophan- and phenylalanine-derived volatiles. MeJA-treated oolong tea was characterized by a more pleasant aroma. Especially the terpenoids linalool and oxides, geraniol, and carvenol increased by more than twofold.
Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sjögren Syndrome

Background and objective: Recessive mutations in the SIL1 gene cause Marinesco-Sjögren syndrome (MSS), a rare neuropediatric disorder. MSS-patients typically present with congenital cataracts, intellectual disability, cerebellar ataxia and progressive vacuolar myopathy. However, atypical clinical presentations associated with SIL1 mutations have been described over the last years; compound heterozygosity of SIL1 missense mutations even resulted in a phenotype not fulfilling the clinical diagnostic criteria of MSS. Thus, a read-out system to evaluate reliably the pathogenicity of amino acid changes in SIL1 is needed. Here, we aim to provide suitable cellular biomarkers enabling the robust evaluation of pathogenicity of SIL1 mutations. Methods: Five SIL1 variants including one polymorphism (p.K132Q), three known pathogenic mutations (p.V231_I232del, p.G312R, and p.L457P) and one ambiguous missense variant (p.R92W) were studied along with the wild-type proteins in Hek293 in vitro models by cell biological assays, immunoprecipitation, immunoblotting, and immunofluorescence as well as electron microscopy. Moreover, the SIL1-interactomes were interrogated by tandem-affinity-purification and subsequent mass spectrometry. Results: Our combined studies confirmed the pathogenicity of p.V231_I232del, p.G312R, and p.L457P by showing instability of the proteins as well as tendency to form aggregates. This observation is in line with altered structure of the ER-Golgi system and vacuole formation upon expression of these pathogenic SIL1-mutants as well as the presence of oxidative or ER-stress. Reduced cellular fitness along with abnormal mitochondrial architecture could also be observed. Notably, both the polymorphic p.K132Q and the ambiguous p.R92W variants did not elicit such alterations. Study of the SIL1-interactome identified POC1A as a novel binding partner of wild-type SIL1; the interaction is disrupted upon the presence of pathogenic mutants but not influenced by the presence of benign variants. Disrupted SIL1-POC1A interaction is associated with centrosome disintegration. Conclusions: We developed a combination of cellular outcome measures to evaluate the pathogenicity of SIL1 variants in suitable in vitro models and demonstrated that the p. R92W missense variant is a polymorphism rather than a pathogenic mutation leading to MSS.
Prevention, assessment and treatment of suicidal behavior

Suicide is a serious global public health problem in both devel- oped and developing countries. The results of the research reviewed here reveal that, in Europe, one person dies by suicide every nine minutes, and that in Spain it is currently the leading external cause of death after road traffic accidents. This review presents the latest findings on effective sui- cide prevention strategies in the general population as well as the most ap- propriate instruments for assessing the level of risk for suicidal behavior in a clinical population. The treatments that scientific literature reports to be most effective in the fight against suicide are also included. The most sig- nificant results in terms of both prevention and treatment are discussed and several limitations to this study are also raised, which may be consid- ered for future work by practitioners and researchers interested in this field.
When Error Learning Orientation Leads to Learning From Project Failure: The Moderating Role of Fear of Face Loss

As the importance of failure is widely recognized, there is increasing research interest in the antecedents of learning from failure. Basing on affective event theory, the current study cast light on individuals’ cognition of error and proposed that employees with higher levels of error learning orientation tend to show more positive grieving after project failure, which in turn increases their learning from failure. Using a sample of 752 employees from 140 project teams, we found empirical evidence to support this theoretical model. Our results indicated that positive grieving mediated the relationship between error learning orientation and learning from failure. Besides, the relationship between error learning orientation and positive grieving is more positive for employees with lower levels of fear of face loss. Our findings help enrich the antecedents of learning from failure by shedding light on how and when error learning orientation matters. Theoretical and practical implications are discussed.
Immobilization Decreases FOXO3a Phosphorylation and Increases Autophagy-Related Gene and Protein Expression in Human Skeletal Muscle

Immobilization of the lower limbs promotes a catabolic state that reduces muscle mass, whereas physical training promotes an anabolic state that increases muscle mass. Understanding the molecular mechanisms underlying this is of clinical interest, as loss of muscle mass is a major complication to critical illness in humans. To determine the molecular regulation of protein synthesis and degradation during muscle loss and hypertrophy, we examined skeletal muscle biopsies from healthy human subjects after 2 weeks unilateral immobilization of a lower limb and during 6 weeks of physical rehabilitation. We have previously shown that cross-sectional area of the knee muscle-extensors decreased by ∼10% during immobilization and was completely restored during rehabilitation. Here we provide novel data to suggest that autophagy is an important underlying mechanism involved in regulation of muscle mass. Protein expression of MuRF1 and ATROGIN-1 did not change during the study, indicating that the recruitment of substrates to the proteasomes was unaltered. Phosphorylation of mTORat Ser2448 did not change during the study, and neither did phosphorylation of the mTORC1 substrates 4EBP1 Thr37/46 and p70S6K Thr389, suggesting that this pathway does not suppress protein synthesis during muscle wasting. Protein levels of p62 and ULK1 increased during immobilization and returned to baseline levels during rehabilitation. Same pattern was observed for FOXO3a phosphorylation at Ser318/321, suggesting transcriptional activation during immobilization and inactivation during rehabilitation. To investigate this further, we analyzed mRNA expression of seven autophagy-related genes controlled by FOXO3a. Five of these (p62, LC3B, BECLIN-1, ATG12, and BNIP3) increased during immobilization and returned to baseline during rehabilitation. In conclusion, immobilization of a lower limb increases autophagy-related gene and protein expression in human skeletal muscle in a pattern that mirrors FOXO3a phosphorylation. These findings could imply that FOXO3a dependent transcriptional regulation of autophagy is involved in the regulation of muscle mass in humans. Clinical Trial Registration The study was approved by the Ethics Committee of Copenhagen (j.no. H-1-2010-016).
Increased Pain Sensitivity in Obese Patients After Lung Cancer Surgery

Background: Obesity and cancer are recognized worldwide health threats. While there is no reported causal relationship, the increasing frequency of both conditions results in a higher incidence of obese patients who are being treated for cancer. Physiological data indicate that there is a relationship between obesity and susceptibility to pain; however, currently, there are no specific pharmacological interventions. Objective: To evaluate the self-reported intensity of postoperative pain in obese and nonobese lung cancer who receive either thoracotomy or video-assisted thoracic surgery (VATS) surgical therapy. Material and Methods: In 50 obese [mean body mass index (BMI) of 34.1 ± 3.2 kg/m2] and 62 nonobese (mean BMI of 24.9 ± 3 kg/m2) lung cancer patients, the intensity of pain was estimated every 4 h using a visual analog scale (VAS, 0 indicating no pain and 10 indicating “worst imaginable pain”) beginning shortly after surgery (Day O) and continuing until the day of discharge (Day D). Results: The self-reported pain was more severe in obese than in nonobese patients, both at the time of the operation [Day O (4.5 ± 1.2 vs 3.4 ± 1.1; p < 0.0001)] and at the day of discharge [Day D (3.9 ± 1.4 vs 2.6 ± 0.9, p < 0.0001)]. This finding was consistent both in the patients after thoracotomy and after video-assisted thoracic surgery (VATS, p < 0.0001). The patients with severe pain shortly after surgery (VAS score >4) had significantly higher BMI (31.8 ± 5.6 kg/m2 vs 28.8 ± 5.2 kg/m2, p < 0.01) and were hospitalized longer than the remaining patients (13.0 ± 13.6 days vs 9.5 ± 3.6 days, p < 0.05). Conclusion: The reported perception of pain in obese lung cancer patients is greater than in nonobese patients undergoing the same thoracic surgery. In obese patients, severe pain persisted longer. Pain management is an important consideration in the postoperative care of lung cancer patients, even more so with obese patients.
Rhenium-188 Labeled Radiopharmaceuticals: Current Clinical Applications in Oncology and Promising Perspectives

Rhenium-188 (188Re) is a high energy beta-emitting radioisotope with a short 16.9 h physical half-life, which has been shown to be a very attractive candidate for use in therapeutic nuclear medicine. The high beta emission has an average energy of 784 keV and a maximum energy of 2.12 MeV, sufficient to penetrate and destroy targeted abnormal tissues. In addition, the low-abundant gamma emission of 155 keV (15%) is efficient for imaging and for dosimetric calculations. These key characteristics identify 188Re as an important therapeutic radioisotope for routine clinical use. Moreover, the highly reproducible on-demand availability of 188Re from the 188W/188Re generator system is an important feature and permits installation in hospital-based or central radiopharmacies for cost-effective availability of no-carrier-added (NCA) 188Re. Rhenium-188 and technetium-99 m exhibit similar chemical properties and represent a “theranostic pair. ” Thus, preparation and targeting of 188Re agents for therapy is similar to imaging agents prepared with 99mTc, the most commonly used diagnostic radionuclide. Over the last three decades, radiopharmaceuticals based on 188Re-labeled small molecules, including peptides, antibodies, Lipiodol and particulates have been reported. The successful application of these 188Re-labeled therapeutic radiopharmaceuticals has been reported in multiple early phase clinical trials for the management of various primary tumors, bone metastasis, rheumatoid arthritis, and endocoronary interventions. This article reviews the use of 188Re-radiopharmaceuticals which have been investigated in patients for cancer treatment, demonstrating that 188Re represents a cost effective alternative for routine clinical use in comparison to more expensive and/or less readily available therapeutic radioisotopes.
An HST/ACS view of the inhomogeneous outer halo of M31

We present a high precision photometric view of the stellar populations in the outer halo ofM31, using data taken with the Hubble Space Telescope/Advanced Camera for Surveys. We analyse the field populations adjacent to 11 luminous globular clusters which sample the galactocentric radial range 18 ≤ R ≤ 100 kpc and reach a photometric depth of ∼2.5 mag below the horizontal branch (m F814W ∼ 27 mag). The colour-magnitude diagrams are well populated out to ∼60 kpc and exhibit relatively metal-rich red giant branches, with the densest fields also showing evidence for prominent red clumps. We use the Dartmouth isochrones to construct metallicity distribution functions which confirm the presence of dominant populations with ([Fe/H]) ≈ ―0.6 to -1.0 dex and considerable metallicity dispersions of 0.2 to 0.3 dex (assuming a 10 Gyr population and scaled-solar abundances). The average metallicity over the range 30-60 kpc is [Fe/H] = ―0.80 ± 0.14 dex, with no evidence for a significant radial gradient. Metal-poor stars ([Fe/H] ≤ ―1.3) typically account for ≤ 10―20 per cent of the population in each field, irrespective of radius. Assuming our fields are unbiased probes of the dominant stellar populations in these parts, we find that the M31 outer halo remains considerably more metal rich than that of the Milky Way out to at least 60 kpc.
Platelets as Mediators of Thromboinflammation in Chronic Myeloproliferative Neoplasms

Chronic myeloproliferative neoplasms (MPN) are stem cell disorders driven by mutations in JAK2, CALR, or MPL genes and characterized by myeloid proliferation and increased blood cell counts. They encompass three closely related conditions, including essential thrombocythemia, polycythemia vera, and primary myelofibrosis. Elevated levels of cytokines released by clonal and non-clonal cells generate a chronic proinflammatory state that contributes to disease pathogenesis. Thrombosis represents the most common cause of morbidity and mortality in MPN, although paradoxically, patients may also present with a bleeding diathesis. The mechanisms leading to thrombosis are complex and multiple and include increased blood cells together with qualitative abnormalities of red cells, leukocytes, and platelets that favor a prothrombotic activated phenotype. The functional interplay between blood cells, the clotting cascade, and dysfunctional endothelium contributes to hypercoagulability and this process is perpetuated by the effect of inflammatory cytokines. In addition to their well-known function in hemostasis, platelets contribute to innate immunity and inflammation and play a key role in MPN thromboinflammatory state. In vivo platelet activation leads to platelet aggregate formation and exposure of adhesion molecules which favor their interaction with activated neutrophils and monocytes leading to circulating platelet-leukocyte heterotypic aggregates. Platelets are recruited to the activated endothelium further enhancing the reciprocal activation of both cell types. Crosstalk between activated cells drives cytokine production, further fuelling the self-reinforcing thromboinflammatory loop. In addition, MPN platelets provide a procoagulant scaffold which triggers the coagulation cascade and platelet-derived microparticles amplify this response. Markers of platelet, leukocyte, endothelial and coagulation activation are increased in MPN patients although prospective studies are required to determine the potential value of these parameters for identifying patients at increased thrombotic risk. Thrombosis remains the main complication of MPN patients, with a high risk of recurrence despite adequate cytoreductive and antithrombotic treatment. Deeper insight into the mechanism favoring thrombosis development in this setting may lead to novel therapeutic approaches for MPN thrombosis. Considering the critical role of inflammation in the vascular risk, concomitant targeting of inflammatory pathways could potentially impact on primary or secondary prevention strategies.
No Strong Evidence of Priming Effects on the Degradation of Terrestrial Plant Detritus in Estuarine Sediments

EG was funded by the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS was funded by the Scottish Funding Council (Grant reference HR09011) and contributing institutions. EG is currently funded by the Hellenic Foundation for Research and Innovation (HFRI) (grant number 1874) and the General Secretariat for Research and Technology (GSRT). This study was funded by the MASTS Marine Biogeochemistry Forum small grants scheme and their support is gratefully acknowledged.
Effect of High-Intensity Exercise on Multiple Sclerosis Function and 31P MRS Outcomes.

PURPOSE We determined if a high-intensity aerobic exercise program would be safe, improve expected fitness and clinical outcomes, and alter exploratory phosphorous magnetic resonance spectroscopy (P MRS) outcomes in persons with multiple sclerosis (PwMS). METHODS This open-label prospective pilot study compared 2 cohorts of ambulatory PwMS matched for age, sex and VO2max. Cohorts underwent 8-weeks of high-intensity aerobic exercise (MS-Ex, n=10) or guided stretching (MS-Ctr, n=7). Aerobic exercise consisted of four 30-minute sessions per week while maintaining >70% maximal heart rate. Changes in cardiorespiratory fitness, clinical outcomes, and P MRS of tibialis anterior muscle (TA) and brain were compared. Cross-sectional P MRS comparisons were made between all MS participants and a separate matched healthy control (HC) population. RESULTS The MS-Ex cohort achieved target increases in VO2max (mean +12.7%, p=<0.001, between-group improvement p=0.03). One participant was withdrawn for exercise-induced syncope. The MS-Ex cohort had within-group improvements in fat mass (-5.8%, p=0.04), lean muscle mass (+2.6%, p=0.02), Symbol Digit Modalities Test (+15.1%, p=0.04), and cognitive subscore of the Modified Fatigue Impact Scale (MFIS: -26%, p=0.03) while only the physical subscore of the MFIS improved in MS-Ctr (-16.1%, p=0.007). P MRS revealed significant within-group increases in MS-Ex participants in TA rate-constant of PCr recovery (kPCr; +31.5%, p=0.03) and ATP/PCr (+3.2%, p=0.01), and near significant between-group increases in TA kPCr (p=0.05) but no significant changes in brain P MRS following exercise. Cross-sectional differences existed between MS and HC brain PCr/Pi (4.61 ± 0.44, 3.93 ± 0.19 p=0.0019). CONCLUSION High-intensity aerobic exercise in PwMS improved expected cardiorespiratory and clinical outcomes but provoked one serious adverse event. P MRS may serve to explore underlying mechanisms by which aerobic exercise exerts cerebral benefits.
The Comprehension of Counterfactual Conditionals: Evidence From Eye-Tracking in the Visual World Paradigm

Three experiments tracked participants’ eye-movements to examine the time course of comprehension of the dual meaning of counterfactuals, such as “if there had been oranges then there would have been pears. ” Participants listened to conditionals while looking at images in the visual world paradigm, including an image of oranges and pears that corresponds to the counterfactual’s conjecture, and one of no oranges and no pears that corresponds to its presumed facts, to establish at what point in time they consider each one. The results revealed striking individual differences: some participants looked at the negative image and the affirmative one, and some only at the affirmative image. The first experiment showed that participants who looked at the negative image increased their fixation on it within half a second. The second experiment showed they do so even without explicit instructions, and the third showed they do so even for printed words.
Antidiabetic, antihyperlipidemic and histopathological analysis of Panax quinquefolium extract on alloxan induced diabetic rats

Objective: To investigate antidiabetic, hypolipidemic histopathological analysis of Panax quinquefolium aqueous (AGE) extract in alloxan induced diabetic rat by administering intraperitoneal dose (100mg/kg bodyweight / day). Methods: Optimal cutoff level of each of the four plasma glucose values of oral glucose tolerance test in alloxan diabetic rat was done. Other parameters as liver profile, renal profile and total lipid levels were determined in normal and alloxan induced diabetic rats after intraperitoneal administration of the extract for 30 days. Histopathological changes in diabetic rat organs (pancreas, liver and kidneywere also observed after extract treatment. Results: Daily intraperitoneal administration AGE (100 mg/kg body weight) and glimebride (10 microg/g body wt) showed beneficial effects on blood glucose level (P<0.001) as well as improving kidney, liver functions and hyperlipedemia due to diabetes. The extract treatment also showed enhanced serum insulin level and body weight of diabetic rats as compared to diabetic control group. Furthermore, the extract has a favorable effect on the histopathological changes of the pancreas, liver and kidney in alloxan induced diabetes. Conclusion: Panax quinquefoliumposses antidiabetic property as well as improve bodyweight, liver profile, renal profile and total lipid levels. AGE has also favorable effect to inhibit the histopathological changes of the pancreas and kidney in alloxan induced diabetes.
Effect of Preoperative Bisphosphonate Treatment on Fracture Healing after Internal Fixation Treatment of Intertrochanteric Femoral Fractures

Purpose There are concerns that administration of bisphosphonate (BP) can substantially suppress bone turnover, potentially interfering with fracture healing. We investigated the effects of preoperative BP administration before internal fixation of intertrochanteric femoral fractures using fracture healing and clinical outcomes. Materials and Methods We retrospectively analyzed data from 130 patients who underwent internal fixation for osteoporotic intertrochanteric femoral fractures between March 2012 and July 2016. Patients previously treated with BPs for at least 3 months (BP group; n=29) were compared with the remaining patients (BP-naïve group; n=101). Radiographs were used to assess and compare fracture healing 3 months and 1 year postsurgery. The primary clinical outcome measure assessed was change in Koval score. Results Fracture union at 3 months after surgery was verified in 72.4% of patients (21/29) in the BP group and 90.1% of patients (91/101) in the BP-naïve group (P=0.027). Fracture union at 1 year postsurgery (BP group, 93.1% [27/29] vs. BP-naïve group, 97.0% [98/101], P=0.310) and change in Koval score (1.1 vs. 1.0, P=0.694) were not significantly different between the groups. Multivariable logistic regression analysis revealed that a history of BP administration was associated with an increased risk of delayed union at 3 months postsurgery (P=0.014). Conclusion Preoperative administration of BP was associated with a decreased fracture healing rate 3 months after internal fixation, compared with BP-naïve patients. Therefore, patients previously treated with a BP should be carefully allowed to wean off walking aids and transition to full weight-bearing in the early postoperative period.