Miracle-dz/UniFAR-FTUs huggingface dataset:
Stimulation strategies for selective activation of retinal ganglion cell soma and threshold reduction

Objective. Retinal prosthetic implants restore partial vision to patients blinded due to outer retinal degeneration, using a camera-guided multielectrode array (MEA) that electrically stimulates surviving retinal neurons. Commercial epi-retinal prostheses use millisecond-scale charge-balanced, symmetric, cathodic-first biphasic pulses to depolarize retinal ganglion cells (RGCs) and bipolar cells (BCs), frequently creating oblong perceptions of light related to axonal activation of RGCs. Stimulation strategies that avoid axonal stimulation and decrease the threshold of targeted neurons may significantly improve prosthetic vision in terms of spatial resolution and power efficiency. Approach. We developed a virus-transduced genetically encoded calcium indicator (GECI) GCaMP6f and microscopy platform for calcium imaging to record the neural activity from RGCs at single-cell resolution in wholemount retinas. Multiple stimulation paradigms were applied through a microelectrode array (MEA) with transparent indium tin oxide electrodes. The evoked neuronal activities were converted to corresponding 2D calcium imaging transient pattern and spatial threshold map to identify the ideal focal response which corresponds to optimal percept in patient. Main results. The proposed optical system with GCaMP6f is capable of recording from population of mouse RGCs in real time during electrical stimulation with precise location information relative to the stimulation sites. Optimal duration and phase order of pulse were identified to avoid axonal stimulation and selectively activate targeted RGC somas, without requiring a significant increase in stimulation charge. Additionally, we show that reduced stimulus threshold can be achieved with the special design of asymmetric anodic-first pulse. Significance. Our findings support the possibility of manipulating the responses of RGCs through varying the stimulation waveform. Focal response can be achieved with relative short duration (⩽120 μs) pulses, and can be improved by reversing the standard phase order. The RGCs threshold can be significantly reduced by 33.3%–50% in terms of charge through applying hyperpolarizing pre-pulses with a 20:1 ratio (pre-pulse:stimulus pulse). The results support the future retinal prosthesis design that potentially forms more ideal shape perception with higher spatial resolution and power efficiency.
Extending the Technology Acceptance Model for Use of e-Learning Systems by Digital Learners

Technology-based learning systems enable enhanced student learning in higher-education institutions. This paper evaluates the factors affecting behavioral intention of students toward using e-learning systems in universities to augment classroom learning. Based on the technology acceptance model, this paper proposes six external factors that influence the behavioral intention of students toward use of e-learning. A quantitative approach involving structural equation modeling is adopted, and research data collected from 437 undergraduate students enrolled in three academic programs is used for analysis. Results indicate that subjective norm, perception of external control, system accessibility, enjoyment, and result demonstrability have a significant positive influence on perceived usefulness and on perceived ease of use of the e-learning system. This paper also examines the relevance of some previously used external variables, e.g., self-efficacy, experience, and computer anxiety, for present-world students who have been brought up as digital learners and have higher levels of computer literacy and experience.
Integrated Sliding Mode Control and Neural Networks Based Packet Disordering Prediction for Nonlinear Networked Control Systems

In this paper, we propose a new scheme based on neural networks for predicting the packet disordering and sliding mode control (SMC) to stabilize the nonlinear networked control systems (NCSs). It is assumed that the packet disordering is unknown in the NCSs. The stochastic configuration networks (SCNs), which randomly assign the input weights and biases and analytically evaluate the output weights, are designed to solve the problem of unknown packet disordering. A new SMC scheme is developed by integrating the SCNs algorithm to learn and control the system in advance. Specifically, a novel measurement of packet disordering is constructed for the quantization of the packet disordering. In addition, the newest signal principle leads to the existence of stochastic parameters, thereby resulting in a Markovian jumping system. The effectiveness of the proposed approach is verified by some simulation results.
Whole-genome sequencing in a family with twin boys with autism and intellectual disability suggests multimodal polygenic risk

Over the past decade, a focus on de novo mutations has rapidly accelerated gene discovery in autism spectrum disorder (ASD), intellectual disability (ID), and other neurodevelopmental disorders (NDDs). However, recent studies suggest that only a minority of cases are attributable to de novo mutations, and instead these disorders often result from an accumulation of various forms of genetic risk. Consequently, we adopted an inclusive approach to investigate the genetic risk contributing to a case of male monozygotic twins with ASD and ID. At the time of the study, the probands were 7 yr old and largely nonverbal. Medical records indicated a history of motor delays, sleep difficulties, and significant cognitive deficits. Through whole-genome sequencing of the probands and their parents, we uncovered elevated common polygenic risk, a coding de novo point mutation in CENPE, an ultra-rare homozygous regulatory variant in ANK3, inherited rare variants in NRXN3, and a maternally inherited X-linked deletion situated in a noncoding regulatory region between ZNF81 and ZNF182. Although each of these genes has been directly or indirectly associated with NDDs, evidence suggests that no single variant adequately explains the probands’ phenotype. Instead, we propose that the probands’ condition is due to the confluence of multiple rare variants in the context of a high-risk genetic background. This case emphasizes the multifactorial nature of genetic risk underlying most instances of NDDs and aligns with the “female protective model” of ASD.
Regularizing Forward and Backward Decoding to Improve Neural Machine Translation

The common recurrent neural network (RNN) based sequential decoder of the neural machine translation (NMT) model can only translate from one direction, which makes the model overfit in the forward direction and leaves backward information of target sentences unexploited. We propose to use a regularization loss to encourage NMT decoder to exploit bidirectional information of target sentences. Beside of forward decoding, we train an extra set of decoding components to predict translation from backward, and use regularization to enforce the forward and backward hidden states at the same time step have connection. During training phase, the forward hidden states can encode future information from the backward hidden states; while during test phase, we only use the enhanced forward decoding components to translate. Our empirical experiments demonstrated that our approach can significantly improve the performance on WMT German-English and English-Chinese translation tasks in terms of NIST and BLEU score.
Measurement of Antiproton Production in p-He Collisions at sqrt[s_{NN}]=110  GeV.

The cross section for prompt antiproton production in collisions of protons with an energy of 6.5 TeV incident on helium nuclei at rest is measured with the LHCb experiment from a data set corresponding to an integrated luminosity of 0.5 nb^{-1}. The target is provided by injecting helium gas into the LHC beam line at the LHCb interaction point. The reported results, covering antiproton momenta between 12 and 110 GeV/c, represent the first direct determination of the antiproton production cross section in p-He collisions, and impact the interpretation of recent results on antiproton cosmic rays from space-borne experiments.
Injury Risk and Injury Burden Are Related to Age Group and Peak Height Velocity Among Talented Male Youth Soccer Players

Background: The relationship between injury risk (IR) in age groups and periods around peak height velocity (PHV) remains unclear. PHV is defined as the moment of the largest increase in body height. Purpose: To investigate injury risk and injury burden as functions of growth velocity (periods around PHV) and chronological age groupings (under 12 years [U12] to U19) in talented youth male soccer players. Study Design: Cohort study; Level of evidence, 2. Methods: A total of 170 players from the youth academy of a Dutch soccer club (highest professional league: Eredivisie) were observed for 1 to 3 seasons. Injuries, exposure, PHV age, and chronological age were registered. The injury incidence density (IID) and injury burden per 1000 hours of soccer participation, with 95% CIs, were calculated for 5 PHV periods and 7 age groups. These were compared with the overall cohort results using incidence ratios (IRs) and burden ratios (BRs) with 95% CIs. Results: The mean age at PHV was 14.4 ± 0.65 years (range, 12.8-16.5 years). The mean IID for the total cohort was 8.34 injuries per 1000 hours (95% CI, 7.71-9.02). Compared with the overall mean, a significantly higher IID was found for PHV period 4+5 (IR, 1.31 [95% CI, 1.00-1.71]; P = .049) and for the U15 group (IR, 1.49 [95% CI, 1.24-1.79]; P < .001). The overall injury burden was 58.37 injury days per 1000 hours (95% CI, 56.66-60.13). In PHV period 4+5, the injury burden was significantly higher (BR, 1.53 [95% CI, 1.39-1.68]; P < .001) when compared with the overall mean. Also, compared with the overall mean, the injury burden was higher in the U16 (BR, 1.48 [95% CI, 1.39-1.58]; P < .001), U15 (BR, 1.28 [95% CI, 1.19-1.38]; P < .001), and U17 groups (BR, 1.21 [95% CI, 1.13-1.31]; P < .001). Conclusion: Talented young soccer players were more prone to injuries during the 6 months after PHV (31% above overall mean) as well as in the U15 group (49% above overall mean). Based on the higher injury burden in the U16 (48%), U15 (28%), and U17 (21%) groups, we suggest that research on injury risk factors and preventive measures should primarily target these age groups. Additional interventions based on PHV may be of limited value from a screening perspective. Further research is needed on the interaction between age groups and PHV periods.
Forced Swim Reliability for Exercise Testing in Rats by a Tethered Swimming Apparatus

To assess the physical capacity of rats in forced swim tests, the animal should perform a continuous activity (CON) at the surface to avoid apnea. Bobbing movement (BOB), vigorous paddling known as climbing (CLI), and diving activity (DIV) are inadequate swimming patterns known to increase the exercise intensity variability, impairing the test reliability. Thus, the exercise work accomplished and related physiological variables, such as the blood lactate concentration, may be unreproducible in forced swim. This study aimed to verify the exercise work reproducibility in rats with a 30-min test–retest at maximal lactate steady state (MLSS) intensity using a tethered-swimming apparatus that analyzes swimming patterns by the direct measurement of swimming force. Additionally, it was determined the swimming force and duration of CON, BOB, CLI, and DIV at physiologically different exercise-intensities. The swimming force at MLSS (n = 64) was 38 ± 7 gf.Kg-1, while the blood lactate concentration was 4.2 ± 1.6 mmol.L-1. In the test–retest (N = 23), swimming force (36.6 ± 7 gf.Kg-1 vs. 36.4 ± 7 gf.Kg-1) and blood lactate concentration (4.7 ± 1.7 mmol.L-1 vs. 4.2 ± 1.7 mmol.l-1) were similar, but only the swimming force was highly correlated (0.90 and 0.31). Although it was not statistically different, the swimming force for CON tends to be slightly lower than CLI and slightly higher than BOB independently of exercise-intensity. The CON pattern predominates (∼52.8 ± 18%) at intensities below and of MLSS but BOB was the swimming pattern more often observed above MLSS-intensity (52.6 ± 18%). The present study used a tethered swimming apparatus to investigate the reliability of forced swim tests for exercise testing in rats and better understand the swimming patterns when determining the MLSS, but the results can be extended to any study that rely on forced swim for exercise testing and training. The result suggests that, at least at intensities of physiological stability, the exercise work accomplished by rats is reproducible in forced swim, but the blood lactate concentration seems to be affected by other factors, such as the apnea and stress caused by the possibility of drowning, besides the exercise-intensity.
An Integrated Analysis of Risk Factors of Cognitive Impairment in Patients with Severe Carotid Artery Stenosis.

OBJECTIVE To investigate cognitive dysfunction in patients with carotid artery stenosis (CAS) and potential risk factors related to cognitive-especially memory-dysfunction. METHODS Forty-seven patients with carotid artery stenosis were recruited into our study cohort. The Mini-Mental State Examination (MMSE) and the Montreal Cognitive Assessment (MoCA) were adopted to assess cognitive function, the Wechsler Memory Scale (WMS) to assess memory function, high-resolution MRI and enhanced ultrasound to evaluate carotid plaques, and computed tomography perfusion (CTP) imaging to evaluate intracranial blood perfusion. Single-factor analysis and multiple-factor regression analysis were used to analyze potential risk factors of cognitive impairment. RESULTS Mini-Mental State Examination test results showed that 22 patients had cognitive impairment, and MoCA test results showed that 10 patients had cognitive impairment. Analysis of various risk factors indicated that the average memory quotient of female patients was higher than that of males (P = 0.024). The cognitive and memory performance of those with an educational background above high school were significantly better than those of participants with high school or lower (P = 0.045). Patients with abnormal intracranial perfusion performed worse on the MMSE test (P = 0.024), and their WMS scores were significantly lower (P = 0.007). The MMSE scores and the memory quotients were significantly lower in patients with a history of cerebral infarction (MMSE, P = 0.047, memory quotient score, P = 0.018). CONCLUSION A history of cerebral infarction and abnormal cerebral perfusion are associated with decline in overall cognitive function and memory in patients with carotid stenosis. Being female and having an educational background above high school may be protective factors in the development of cognitive dysfunction.
Potential Application of Ixeris dentata in the Prevention and Treatment of Aging-Induced Dry Mouth

Dry mouth is a common complaint among the elderly population. The aim of this study was to investigate the effect of Ixeris dentata (IXD) extract on aging-induced dry mouth. We used young (two months) and aged (20 months) SD rats in our study. Using water as the vehicle, IXD extract (25, 50, and 100 mg/kg) was given via oral gavage to the young and aged rats for eight weeks. We found that the salivary flow rate relative to the submandibular gland weight was differently influenced by IXD extract treatment. IXD extract augmented the submandibular gland acinar cells, which are depleted during aging. In addition, the decreased salivary alpha-amylase, inositol triphosphate receptor, and aquaporin-5 in the aging rats were upregulated by IXD treatment. Free radical-induced oxidative stress in the aging rats was also alleviated in the IXD-treated group. The formation of high molecular weight complexes of protein disulfide isomerase, decreased expression of an ER chaperone (GRP78), and increased ER stress response (ATF-4, CHOP and p-JNK) in aging rats was regulated with IXD treatment, and eventually increased salivary secretions from the aging submandibular glands. These are the first data to suggest that IXD extract might ameliorate aging-associated oral dryness by regulating the ER environment.
Neuroglial Differentiation and Neoplasms in Testicular Germ Cell Tumors Lack Immunohistochemical Evidence of Alterations Characteristic of Their CNS Counterparts: A Study of 13 Cases

Overgrowth of neuroglial tissue is rare in testicular germ cell tumors and mostly reported as isolated cases. We retrospectively reviewed 13 cases of testicular germ cell tumors from 2 institutions from 1995 to 2018. Hematoxylin and eosin slides were collected and reviewed. Immunohistochemistry was performed in all cases with available material. The series included 4 primary tumors and 9 metastases, including 8 retroperitoneal and 1 axillary lymph node (LN). The average age was 34 (range: 19 to 54). Five of the LN dissections were postchemotherapy, with one a recurrence 5 years after the initial diagnosis. The average tumor size for primary tumors was 5.15 cm (range: 1.7 to 7.3) and for metastases was 6.4 cm (range: 0.6 to 15). The largest size of the neuroglial component was 4.5 cm in the primary tumors and 7.5 cm in metastatic sites. The neuroglial component in the primary site was associated with pure teratoma (n=2) and with a mixed germ cell tumor (teratoma, seminoma, and embryonal carcinoma) (n=2). Cases involving LNs were associated with teratoma (n=4), seminoma (n=2), rhabdomyosarcoma (n=2), primitive neuroectodermal tumors (n=1), and high-grade sarcoma (n=1) (some with >1 other component). Two cases were pure glial tumor. Histologically, the neuroglial components included low-grade astrocytoma (n=3) (both with microcysts formation and pilocytic features), gemistocytic astrocytomas (n=3), anaplastic astrocytoma (n=2), ganglioglioma (n=1), glioblastoma (n=2), gliosarcoma (n=1), and developing central nervous system (CNS) (n=2). By immunohistochemistry, 13/13 (100%) cases were GFAP(+), 10/10 (100%) cases showed retained ATRX, 10/10 were IDH1 pR132H (−), 5/10 (50%) were p53 (+). A single case 1/10 (10%) was BRAF p.V600E (+), but a mutation was not identified by polymerase chain reaction. Follow-up was available in 6 patients; 4 were confirmed to have received chemotherapy with BEP; 1 had a local recurrence and the patient with gliosarcoma developed a lung metastasis morphologically similar to the gliosarcoma of the retroperitoneum. In conclusion, neuroglial differentiation and neoplasms are rare in testicular germ cell tumors and are most commonly associated with teratomas; they can be seen in primary and metastatic sites. They exhibit the full range of neuroglial differentiation including developing CNS to gliomas/glioneuronal tumors WHO grades I-IV. None of the cases showed results consistent with ATRX, IDH or BRAF alterations, suggesting they have different oncogenic mechanisms than their CNS counterparts.
S-1 plus cisplatin with concurrent radiotherapy for stage III non-small cell lung cancer

Introduction: The present study aims to assess the efficacy and safety of S-1 plus cisplatin as concurrent chemoradiation (experimental group [EG]) compared with standard concurrent chemoradiation regimens (control group[CG]) in patients with local advanced non-small cell lung cancer. Methods: The Cochrane library, pubmed, and Ovid (elsevier) were retrieved. The included randomized controlled trials (RCT) were evaluated, and the statistical analysis was performed using RevMan 5.3 software. Cochrane handbook was applied to evaluate the methodological quality. Statistical significance was considered as P <. 05. Results: There were 5 randomized control trials identified eligible for the meta-analysis. Meta-analysis of the pooled date suggested that overall survival (OS) (HR, 0.81; 95% CI, 0.58–1.13; P = .21, heterogeneity P = 1.00, I2 = 0%), progressives free survival (PFS) (HR, 0.82; 95% CI, 0.62–1.09; P = .18, heterogeneity P = .83, I2 = 0%) and 1,2,3-year OS (1-year OS: RR 1.03; 95% CI: 0.92–1.15, p = 0.59), (2-year OS: RR 1.14; 95% CI: 0.98–1.34, P = .09), (3 -year OS: RR 1.14; 95% CI: 0.90–1.44, P = .29) were not significantly different. The combination of S-1 and cisplatin had lower grade 3 or 4 leukocytopenia, neutropenia, (RR = 0.54, 95% CI: 0.38–0.75, P = .0003; RR = 0.23,95% CI: 0.14–0.36, P <.00001;, respectively). The rates of nausea, diarrhea, thrombocytopenia, pneumonitis, anorexia, anemia, febrile neutropenia were much the same in the 2 groups (RR = 1.35, 95% CI: 0.68–2.68, P = .38; RR = 1.85, 95% CI: 0.61–5.60, P = .28; RR = 1.67, 95% CI: 0.88–3.17, P = .12; RR = 1.19, 95% CI: 0.44–3.21, P = .73; RR = 1.35, 95% CI: 0.68–2.68, P = .38; RR = 0.86, 95% CI:0.55–1.34, P = .50; RR = 0.63, 95% CI:0.35–1.14, P = .13;, respectively). Conclusions: This meta-analysis of 5 randomized control trails demonstrates that EG results similar OS, PFS, and 1,2,3-year OS, compared with CG, with lower risk of leukocytopenia, neutropenia.
Four-PAM Modulation of Ambient FM Backscattering for Spectrally Efficient Low-Power Applications

Ambient backscatter uses radio frequency signals available in the environment (e.g., radio broadcasting, television, or mobile telephony) to transmit data effectively leading to significant energy and cost efficiency increase. This paper presents a novel wireless tag, which for the first time utilizes 4-pulse amplitude modulation technique to modulate the ambient backscattered FM signals in order to send data to a nearby low-cost software defined radio reader. The tag is based on an RF front-end that uses a single transistor controlled by an ultralow-power microcontroller. The microcontroller includes an analog-to-digital converter for sensing and a digital-to-analog converter for RF front-end control. A proof-of-concept prototype is demonstrated in an indoor environment with the low bit rate of 345 b/s and power consumption $27~\mu \text{W}$ . It operated using a real FM station at 34.5 km away and the tag-to-reader distance was tested at 1 m. The value of energy spent in this modulator was 78.2 nJ/bit at 345 b/s and 27.7 nJ/bit at 10.2 kb/s.
Practical Suitability and Experimental Assessment of Tree ORAMs

Oblivious Random-Access Memory (ORAM) is becoming a fundamental component for modern outsourced storages as a cryptographic primitive to prevent information leakage from a user access pattern. The major obstacle to its proliferation has been its significant bandwidth overhead. Recently, several works proposed acceptable low-overhead constructions, but unfortunately they are only evaluated using algorithmic complexities which hide valuable constants that severely impact their practicality. Four of the most promising constructions are Path ORAM, Ring ORAM, XOR Ring ORAM, and Onion ORAM. However, they have never been thoroughly compared against each other and tested on the same experimental platform. To address this issue, we provide a thorough study and assessment of these recent ORAM constructions and implement them under the same testbed. We perform extensive experiments to provide insights into their performance characteristics, simplicity, and practicality in terms of processing time, server storage, client storage, and communication cost. Our extensive experiments show that despite the claimed algorithmic efficiency of Ring and Onion ORAMs and their judicious limited bandwidth requirements, Path ORAM stands out to be the simplest and most efficient ORAM construction.