Miracle-dz/UniFAR-FTUs huggingface dataset:
B Cells Moderate Inflammatory Progression and Enhance Bacterial Containment upon Pulmonary Challenge with Mycobacterium tuberculosis1

Though much is known about the function of T lymphocytes in the adaptive immune response against Mycobacterium tuberculosis, comparably little is understood regarding the corresponding role of B lymphocytes. Indicating B cells as components of lymphoid neogenesis during pulmonary tuberculosis, we have identified ectopic germinal centers (GCs) in the lungs of infected mice. B cells in these pulmonary lymphoid aggregates express peanut agglutinin and GL7, two markers of GC B cells, as well as CXCR5, and migrate in response to the lymphoid-associated chemokine CXCL13 ex vivo. CXCL13 is negatively regulated by the presence of B cells, as its production is elevated in lungs of B cell-deficient (B cell−/−) mice. Upon aerosol with 100 CFU of M. tuberculosis Erdman, B cell−/− mice have exacerbated immunopathology corresponding with elevated pulmonary recruitment of neutrophils. Infected B cell−/− mice show increased production of IL-10 in the lungs, whereas IFN-γ, TNF-α, and IL-10R remain unchanged from wild type. B cell−/− mice have enhanced susceptibility to infection when aerogenically challenged with 300 CFU of M. tuberculosis corresponding with elevated bacterial burden in the lungs but not in the spleen or liver. Adoptive transfer of B cells complements the phenotypes of B cell−/− mice, confirming a role for B cells in both modulation of the host response and optimal containment of the tubercle bacillus. As components of ectopic GCs, moderators of inflammatory progression, and enhancers of local immunity against bacterial challenge, B cells may have a greater role in the host defense against M. tuberculosis than previously thought.
Neural stem cell therapy aiming at better functional recovery after spinal cord injury

Injury to the spinal cord causes transection of axon fibers and neural cell death, resulting in disruption of the neural network and severe functional loss. Reconstruction of the damaged neural circuits was once considered to be hopeless as the adult mammalian central nervous system has very poor ability to regenerate. For this reason, there is currently no effective therapeutic treatment for spinal cord injury (SCI). However, with recent developments in stem cell research and cell culture technology, regenerative therapy using neural stem cell (NSC) transplantation has rapidly been developed, and this therapeutic strategy makes it possible to rebuild the destroyed neural circuits. In this review, we discuss the recent breakthroughs in NSC transplantation therapy for SCI. Developmental Dynamics 247:75–84, 2018. © 2017 Wiley Periodicals, Inc.
The representation of Aboriginal cultural conceptualisations in an adopted English

The Aboriginal inhabitants of Australia encountered varieties of English as a language of colonisation from 1788. Through processes of nativization, Aboriginal people have made English a valid form of expression of their culture and worldview. For this to happen it was necessary for the input varieties of the language received to be changed in form. This, it is claimed, was achieved through processes of Retention, Elimination, Modification and Extension. According to Cultural Linguistics, these processes would have a cultural/conceptual as well as a linguistic dimension. An attempt is made here to trace the processes and to identify the major conceptual imperatives that drove them. It is argued that these were orientations to Relationship, Experience and Integration, as well as the recognition of the practicalities of communicating as a marginal group with other Australians who used English differently.
Is a ventilator-associated pneumonia rate of zero really possible?

Purpose of review The increasing number of hospitals reporting ventilator-associated pneumonia (VAP) rates at or close to zero begs the question of whether zero should become the national benchmark for VAP. This article explores the significance of very low VAP rates, reviews differences in surveillance and clinical rates, proposes reasons for their discrepancies, and suggests possible objective alternatives for surveillance. Recent findings Surveillance rates of VAP are decreasing, whereas clinical diagnoses and antibiotic prescribing remain prevalent. This growing discrepancy reflects the lack of objective and definitive signs to diagnose VAP. External reporting pressures may be encouraging stricter interpretation of subjective signs and other surveillance initiatives that can artifactually lower rates. It is impossible to disentangle the relative contribution of care improvements versus surveillance effects to currently observed low VAP rates. Summary The increasing mismatch between surveillance rates and clinical diagnoses limits the utility of official VAP rates to estimate disease burden and guide quality improvement. Advocates are advised to consider objective alternatives such as average duration of mechanical ventilation, length of stay, mortality, and antibiotic prescribing. Emerging surveillance definitions that use more objective criteria may better reflect and inform future clinical practice.
Emotional Vulnerability and Coping Styles for Resolving Decisional Conflict

ABSTRACT This investigation supplements the study by D. Bouckenooghe, K. Vanderheyden, S. Mestdagh, and S. van Laethem (2007) on the role of cognitive dispositions in coping patterns for resolving decisional conflict. Literature suggests emotional vulnerabilities may significantly affect decision making. Thus, the present authors assessed the role of trait anxiety and depression in decision coping styles as specified by I. L. Janis and L. Mann's (1977) conflict-theory model. The participants—100 young adults—completed the Taylor Manifest Anxiety Scale (J. A. Taylor, 1953), Beck's Depression Inventory (A. T. Beck, R. A. Steer, & G. M. Garbin, 1988), and the Melbourne Decision-Making Questionnaire (L. Mann et al., 1998), which measures 4 coping strategies: vigilance, buck-passing, procrastination, and hypervigilance. Hierarchical multiple regression analysis, controlling for demographic and lifestyle factors, revealed trait anxiety and depression as significant predictors of procrastination and hypervigilance. Depression failed to predict buck-passing but functioned as an important moderator variable whereby trait anxiety better predicted hypervigilance in nondepressed participants. Consistent with past research, emotional dispositions failed to predict vigilance. Overall, these findings implicate emotional vulnerabilities in the quality of decision making but raise important questions about their unique and conditional effects.
Playing the Women’s Card: How Women Respond to Female Candidates’ Descriptive Versus Substantive Representation

When presented the choice between a male and female candidate, it is commonly assumed that women prefer a female candidate. But as more policy and ideologically diverse women run for office, this assumption may not hold true. Using an experimental design embedded in a nationally representative survey, I test how voters respond to female candidates with ideologies and abortion positions similar and contrary to their own preferences. I find that women, generally, prefer a female candidate, but support for a female candidate among women decreases significantly when she has a contrary ideology or policy position. Whether women prefer descriptive or substantive representation also is conditioned on individual-level characteristics. This study advances our understanding of voters’ responses to female candidates’ varying ideological and issue positions, which is increasingly important as more women run for office. Although women are more likely than men to give female candidates the benefit of the doubt, not just any female candidate will do—she needs to appeal to women on issue and ideological grounds too.
Enzyme Replacement Therapy in Patients Who Have Mucopolysaccharidosis I and Are Younger Than 5 Years: Results of a Multinational Study of Recombinant Human α-l-Iduronidase (Laronidase)

OBJECTIVE. Our objective was to evaluate the safety, pharmacokinetics, and efficacy of laronidase in young, severely affected children with mucopolysaccharidosis I. METHODS. This was a prospective, open-label, multinational study of 20 patients who had mucopolysaccharidosis I and were <5 years old (16 with Hurler syndrome, 4 with Hurler-Scheie syndrome) and were scheduled to receive intravenous laronidase at 100 U/kg (0.58 mg/kg) weekly for 52 weeks. Four patients underwent dosage increases to 200 U/kg for the last 26 weeks because of elevated urinary glycosaminoglycan levels at week 22. RESULTS. Laronidase was well tolerated at both dosages. Investigators reported improved clinical status in 94% of patients at week 52. The mean urinary glycosaminoglycan level declined by ∼50% at week 13 and was sustained thereafter. A more robust decrease in urinary glycosaminoglycan was observed in patients with low antibody levels and those who were receiving the 200 U/kg dosage. On examination, the liver edge was reduced by 69.5% in patients with a palpable liver at baseline and week 52 (n = 10). The proportion of patients with left ventricular hypertrophy decreased from 53% to 17%. Global assessment of sleep studies showed improvement or stabilization in 67% of patients, and the apnea/hypopnea index decreased by 5.8 events per hour (−8.5%) in those with abnormal baseline values. The younger patients with Hurler syndrome (<2.5 years) and all 4 patients with Hurler-Scheie syndrome showed normal mental development trajectories during the 1-year treatment period. CONCLUSIONS. Laronidase seems to be well tolerated and to provide clinical benefit in patients who have severe mucopolysaccharidosis I and are <5 years old. Enzyme replacement therapy is not curative and may not improve all affected organs and systems in individuals when irreversible changes have developed. The long-term clinical outcome and effects of antibodies and laronidase dosing on glycosaminoglycan reduction warrant additional investigation.
Effectiveness of a multidisciplinary rehabilitation program in elderly patients with hip fractures.

BACKGROUND AND PURPOSE Hip fracture is a significant health challenge to the elderly, with a high risk of complications, long hospital stay, and functional deterioration. The purpose of this study was to evaluate the effectiveness of a multidisciplinary rehabilitation program (MRP) in older patients with hip fractures. METHODS A before-and-after quasi-experimental design was used. Data were collected at a large teaching hospital in Taipei. The first 105 consecutive patients admitted received conventional care (control group). The next 50 consecutive patients received physical therapy, nurse-supervised exercise, and discharge planning (intervention group). Subjects were assessed upon admission, on the fourth postoperative day, and on the discharge day. The 155 patients recruited met the following criteria: 60 years of age or older, and hospitalized to receive either closed reduction and internal fixation, or hip arthroplasty. RESULTS Compared with the control group, the intervention group had a shorter average hospital stay (9.9 vs 11.6 days, p = 0.01), earlier ambulation (5.7 vs 6.5 days postoperatively), and a lower rate of bowel incontinence (4.0% vs 23.8%) and pressure sores (14.0% vs 24.8%) during hospitalization. The intervention group experienced less decline in mobility (relative to pre-fracture status) than the control group. No significant difference was found in other outcome variables between the control and intervention groups. CONCLUSIONS The findings of this study indicate that an MRP involving physical therapy, nurse-supervised exercise, and discharge planning positively affects health outcomes in older patients with hip fractures.
Spillovers from US Monetary Policy: Evidence from a Time-varying Parameter GVAR Model

This paper develops a global vector autoregressive (GVAR) model with time-varying parameters and stochastic volatility to analyze whether international spillovers of US monetary policy have changed over time. The proposed model allows assessing whether coefficients evolve gradually over time or are better characterized by infrequent, but large breaks. Our findings point towards pronounced changes in the international transmission of US monetary policy throughout the sample period, especially so for the reaction of international output, equity prices, and exchange rates against the US dollar. In general, the strength of spillovers has weakened in the aftermath of the global financial crisis. Using simple panel regressions, we link the variation in international responses to measures of trade and financial globalization. We find that a broad trade base and a high degree of financial integration with the world economy tend to cushion risks stemming from a foreign shock such as a US monetary policy tightening, whereas a reduction in trade barriers and/or a liberalization of the capital account increase these risks.
Effect of Sodium Valproate on Tardive Dyskinesia

Summary The effect of sodium valproate, a drug which has been demonstrated to increase gamma-aminobutyric acid levels in the CNS, on tardive dyskinesia and psychiatric symptoms was investigated in a double-blind cross-over study on 32 chronic psychiatric patients. The oro-facial dyskinesias were totally or significantly relieved in 17 cases. During the active treatment period the involuntary movements of the extremities and dystonic spasms were also significantly relieved in 7 out of 9 patients. In two patients, however, the extrapyramidal symptoms became slightly worse. A significant improvement was noted in the psychiatric symptoms of 14 out of 32 patients during sodium valproate administration. The psychiatric state of 4 out of 32 patients deteriorated. There was no correlation between the serum concentration of sodium valproate and its effect on the dyskinesia or on the psychiatric symptoms. Some of the elderly subjects showed a slight accumulation of the drug.
2‐hydroxyisocaproic acid is fungicidal for Candida and Aspergillus species

The amino acid derivative 2‐hydroxyisocaproic acid (HICA) is a nutritional additive used to increase muscle mass. Low levels can be detected in human plasma as a result of leucine metabolism. It has broad antibacterial activity but its efficacy against pathogenic fungi is not known. The aim was to test the efficacy of HICA against Candida and Aspergillus species. Efficacy of HICA against 19 clinical and reference isolates representing five Candida and three Aspergillus species with variable azole antifungal sensitivity profiles was tested using a microdilution method. The concentrations were 18, 36 and 72 mg ml−1. Growth was determined spectrophotometrically for Candida isolates and by visual inspection for Aspergillus isolates, viability was tested by culture and impact on morphology by microscopy. HICA of 72 mg ml−1 was fungicidal against all Candida and Aspergillus fumigatus and Aspergillus terreus isolates. Lower concentrations were fungistatic. Aspergillus flavus was not inhibited by HICA. HICA inhibited hyphal formation in susceptible Candida albicans and A. fumigatus isolates and affected cell wall integrity. In conclusion, HICA has broad antifungal activity against Candida and Aspergillus at concentrations relevant for topical therapy. As a fungicidal agent with broad‐spectrum bactericidal activity, it may be useful in the topical treatment of multispecies superficial infections.
MANNHEIMIA (PASTEURELLA) HAEMOLYTICA LEUKOTOXIN UTILIZES CD18 AS ITS RECEPTOR ON BIGHORN SHEEP LEUKOCYTES

Pneumonia caused by Mannheimia (Pasteurella) haemolytica is a highly fatal disease of bighorn sheep (Ovis canadensis). Leukotoxin (Lkt), secreted by M. haemolytica, is an important virulence factor of this organism, and is cytolytic to bighorn sheep leukocytes. Previously, we have shown that CD18, the β subunit of β 2 integrins, serves as the receptor for Lkt on bovine leukocytes. Furthermore, anti-CD18 antibodies inhibit Lkt-induced cytotoxicity of bighorn sheep leukocytes. Therefore, we hypothesized that Lkt utilizes CD18 as its receptor on bighorn sheep leukocytes. Confirmation of bighorn sheep CD18 as a receptor for Lkt requires the demonstration that the recombinant expression of bighorn sheep CD18 in Lkt-nonsusceptible cells renders them susceptible to Lkt. Therefore, we transfected cDNA encoding CD18 of bighorn sheep into a Lkt-nonsusceptible murine cell line. Cell surface expression of bighorn sheep CD18 on the transfectants was tested by flow cytometry with anti-CD18 antibodies. Transfectants stably expressing bighorn sheep CD18 on their surface were subjected to flow cytometric analysis for detection of Lkt binding, and cytotoxicity assays for detection of Lkt-induced cytotoxicity. Leukotoxin bound to the transfectants. More importantly, the transfectants were effectively lysed by Lkt in a concentration-dependent manner, whereas the parent cells were not. These results clearly indicate that M. haemolytica Lkt utilizes CD18 as a receptor on bighorn sheep leukocytes. Identification of CD18 as a receptor for Lkt on bighorn sheep leukocytes should enhance our understanding of the pathogenesis of pneumonia, which in turn should help in the development of control measures against this fatal disease of bighorn sheep.
Organizational Change in the Land-Grant System: A Qualitative Evaluation.

Individuals representing different organizations within the Land-Grant University System (LGUS) were asked to provide their insights on organizational change using a focus group approach. The study focused on three key components to organizational change in the LGUS: what initiates change, the hindrances to change, and what sustains change. Themes were analyzed relative to the existing literature and synthesized into a new conceptual model of organizational change in the LGUS. Results indicated change can be effectively initiated at lower levels of the organization where individuals have the appropriate span of control to drive change. Bureaucratic inertia was identified as the primary hindrance to change in the LGUS. Finally, relationships were identified as most important for sustaining change. Based on the results recommendations suggest that organizations within the LGUS employ a balance between proactive and reactive change and establish a clear communication plan and to ensure the plan emphasizes the respect due to those that will be involved or affected. Furthermore, to mitigate the effects of bureaucratic inertia, any organizational change effort within the LGUS should be accompanied by enough time to analyze the culture and build organizational support and buy-in. Additional recommendations were provided based on the results of the study.
Analytical challenges of extracellular vesicle detection: A comparison of different techniques

The interest in extracellular vesicles (EVs) has grown exponentially over the last decade. Evolving evidence is demonstrating that these EVs are playing an important role in health and disease. They are involved in intercellular communication and have been shown to transfer proteins, lipids, and nucleic acids. This review focuses on the most commonly used techniques for detection of EVs, to include microparticles, 100–1,000 nm in size, and exosomes, 50–100 nm in size. Conventional flow cytometry is the most prevalent technique, but nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), and resistive pulse sensing have also been used to detect EVs. The accurate measurement of these vesicles is challenged by size heterogeneity, low refractive index, and the lack of dynamic measurement range for most of the available technologies. Sample handling during the preanalytical phase can also affect the accuracy of measurements. Currently, there is not one single method which allows phenotyping, sizing, and enumerating the whole range of EVs and, therefore, providing all the necessary information to truly understand the biology of these particles. A combination of methods is probably needed which might also include electron and atomic force microscopy and full RNA, lipid, and protein profiling. © 2015 International Society for Advancement of Cytometry
Accuracy in measurement of distance using limited cone-beam computerized tomography.

PURPOSE The purpose of this study was to evaluate the accuracy of measurement of distance on the images produced by limited cone-beam computerized tomography (CT). MATERIALS AND METHODS Five cadaver mandibles were examined by spiral computerized tomography (SCT) and limited cone-beam computerized tomography (LCBCT). The vertical distance from a reference point to the alveolar ridge was measured by caliper on the sliced mandible, and measurement error on the CT images was calculated in percentages based on the actual values and the measurement values obtained from the CT images. RESULTS Measurement error was determined to range from 0 to 1.11 mm (0% to 6.9%) on SCT and from 0.01 to 0.65 mm (0.1% to 5.2%) on LCBCT, with measurement errors of 2.2% and 1.4%, respectively (P < .0001). DISCUSSION This study suggests that distance can be measured accurately using LCBCT. The size of the rectangular solid images obtained using LCBCT (30 mm wide and 42.7 mm long) is thought to be adequate for observation of mandibular bony structure and for preoperative assessment before dental implant placement. CONCLUSION In this experiment on cadaver mandibles, LCBCT was shown to be a useful tool for preoperative evaluation in dental surgery because the relatively small field size of its images limits the patient's exposure to radiation.
Effects of Government Spending on Employment: Evidence from Winners and Runners-Up in Procurement Auctions

To estimate demand for labor, we use a combination of detailed employment data and the outcomes of procurement auctions, and compare the employment of the winner of an auction with the employment of the second ranked firm (i.e. the runner-up firm). Assuming similar ex-ante winning probabilities for both firms, we may view winning an auction as an exogenous shock to a firm's production and its demand for labor. We utilize daily data from almost 900 construction firms and about 3,000 auctions in Austria in the time period 2006 until 2009. Our main results show that the winning firm significantly increases labor demand in the weeks following an auction but only in the years before the recent economic crisis. It employs about 80 workers more after the auction than the runner-up firm. Most of the adjustment takes place within one month after the demand shock. Winners predominantly fire fewer workers after winning than runner-up firms. In the crisis, however, firms do not employ more workers than their competitors after winning an auction. We discuss explanations like labor hoarding and productivity improvements induced by the crisis as well discuss implications for fiscal and stimulus policy in the crisis.
Photo-Accentuated Eruption and Vascular Deposits of Immunoglobulin A Associated with Hepatitis A Virus Infection

Skin rash associated with hepatitis A virus infection has rarely been reported. We describe a patient with hepatitis A virus infection who presented a rubelliform rash markedly accentuated in sun-exposed areas; direct immunofluorescence studies of the lesion revealed immunoglobulin (Ig) A deposition on the endothelial cells in the upper dermis. Oral rechallenge tests of the previously administered drugs failed to reproduce the eruption. The preferential setting of immune complexes containing IgA at sites of sun exposure and sunlight as a triggering factor might have been responsible for the development of the eruption in this patient. Eruptions associated with hepatitis A virus infections may be more frequent than commonly thought. Because of difficulty to exclude the possibility of drug eruptions, these cases might have been overlooked. In patients with such a disorder, a careful clinical workup such as IgM antibodies for hepatitis A virus at diagnosis and during follow-up is especially recommended.