Miracle-dz/UniFAR-FTUs huggingface dataset:
βArrestin1 Regulates the Guanine Nucleotide Exchange Factor RasGRF2 Expression and the Small GTPase Rac-mediated Formation of Membrane Protrusion and Cell Motility*

Background: G protein-coupled receptors (GPCRs) and βarrestins have been shown to regulate cell motility. Results: βArrestin1 regulates cell migration through RasGRF2 (a dual guanine nucleotide exchange factor) gene expression and the small GTPase Rac activity. Conclusion: βArrestin1 may regulate cellular functions at the gene expression level. Significance: βArrestins may function at transcriptional and post-translational levels to regulate cell migration. βArrestin proteins shuttle between the cytosol and nucleus and have been shown to regulate G protein-coupled receptor signaling, actin remodeling, and gene expression. Here, we tested the hypothesis that βarrestin1 regulates actin remodeling and cell migration through the small GTPase Rac. Depletion of βarrestin1 promotes Rac activation, leading to the formation of multipolar protrusions and increased cell circularity, and overexpression of a dominant negative form of Rac reverses these morphological changes. Small interfering RNA library screen identifies RasGRF2 as a target of βarrestin1. RasGRF2 gene and protein expression levels are elevated following depletion of βarrestin1, and the consequent activation of Rac results in dephosphorylation of cofilin that can promote actin polymerization and formation of multipolar protrusions, thereby retarding cell migration and invasion. Together, these results suggest that βarrestin1 regulates rasgrf2 gene expression and Rac activation to affect membrane protrusion and cell migration and invasion.
Thermocapillary flows and interface deformations produced by localized laser heating in confined environment

The deformation of a fluid-fluid interface due to the thermocapillary stress induced by a continuous Gaussian laser wave is investigated analytically. We show that the direction of deformation of the liquid interface strongly depends on the viscosities and the thicknesses of the involved liquid layers. We first investigate the case of an interface separating two different liquid layers while a second part is dedicated to a thin film squeezed by two external layers of same liquid. These results are predictive for applications fields where localized thermocapillary stresses are used to produce flows or to deform interfaces in presence of confinement, such as optofluidics.
Post Hoc Analysis Decisions Drive the Reported Reading Time Effects in Hackl, Koster-Hale & Varvoutis (2012)

Hackl, Koster-Hale & Varvoutis (2012; hereafter HKV) provide data that suggest that in a null context, antecedent-contained deletion (ACD) relative clause structures modifying a quantified object noun phrase (NP) are easier to process than those modifying a definite object NP. HKV argue that this pattern of results supports a quantifier-raising (QR) analysis of both ACD structures and quantified NPs in object position: under the account they advocate, both ACD resolution and quantified NPs in object position require movement of the object NP to a higher syntactic position. The processing advantage for quantified object NPs in ACD is hypothesized to derive from the fact that—at the point where ACD resolution must take place—the quantified NP has already undergone QR, whereas this is not the case for definite NPs. Here, we question these conclusions. In particular, our analyses of HKV’s reading time data reveal several unreported choice points, errors and concerns regarding multiple comparisons in the original HKV data analysis. Importantly, most other plausible ways to analyze these data that we describe here result in the crucial interaction being non-significant. Putting this observation together with the failure to observe the crucial interaction in Gibson & Levy (2016), we conclude that the experiments reported by HKV should not be viewed as providing evidence for the ACD quantifier-raising processing effect. VC The Author, 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com Journal of Semantics, 2017, 1–8 doi: 10.1093/jos/ffx001
Marital satisfaction and father involvement during the transition to parenthood

Studies on the relationship between fathers’ marital satisfaction and involvement with their children have yielded conflicting results. As a partial test of a theoretical model of responsible fathering (Doherty, Kouneski, & Erickson, 1998), this study re-examined this relationship by using measures of both quantity and quality of father involvement and by exploring moderator effects and linear versus curvilinear relationships between the variables. Data were from a longitudinal study of 165 couples collected during the second trimester of pregnancy, and 6 and 12 months postpartum. Bivariate correlations, hierarchical multiple regression, and curve estimation were used to analyze the data. Results showed that fathers’ marital satisfaction and involvement are positively related, that mothers’ employment status and fathers’ attitudes toward father involvement are important moderators, and that the statistical relationships are linear.
Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock

ABSTRACT Background: There are two opposing possibilities for the main pathogenesis of trauma-induced coagulopathy: an acute coagulopathy of trauma shock and disseminated intravascular coagulation with the fibrinolytic phenotype. Objective: The objective of this study was to clarify the main pathogenesis of trauma-induced coagulopathy using a rat model of Noble-Collip drum trauma. Methods: Eighteen rats were divided into the control, trauma 0, and trauma 30 groups. The trauma 0 and 30 groups were exposed to Noble-Collip drum trauma. Blood samples were drawn without, immediately after, and 30 min after Noble-Collip drum trauma in the control, trauma 0, and trauma 30 groups, respectively. Coagulation and fibrinolysis markers were measured. Thrombin generation was assessed according to a calibrated automated thrombogram. Results: Spontaneous thrombin bursts resulting from circulating procoagulants were observed in the nonstimulated thrombin generation assay immediately after trauma. Soluble fibrin levels (a marker of thrombin generation in the systemic circulation) were 50-fold greater in the trauma groups than in the control group. The resultant coagulation activation consumed platelets, coagulation factors, and antithrombin. Endogenous thrombin potential and factor II ratio were significantly negatively correlated with antithrombin levels, suggesting insufficient control of thrombin generation by antithrombin. High levels of active tissue-type plasminogen activator induced hyperfibrin(ogen)olysis. Soluble thrombomodulin increased significantly. However, activated protein C levels did not change. Conclusions: The systemic thrombin generation accelerated by insufficient antithrombin control leads to the consumption of platelets and coagulation factors associated with hyperfibrin(ogen)olysis. These changes are collectively termed disseminated intravascular coagulation with the fibrinolytic phenotype.
Prosody contour prediction with long short-term memory, bi-directional, deep recurrent neural networks

Deep Neural Networks (DNNs) have been shown to provide state-of-the-art performance over other baseline models in the task of predicting prosodic targets from text in a speechsynthesis system. However, prosody prediction can be affected by an interaction of short- and long-term contextual factors that a static model that depends on a fixed-size context window can fail to properly capture. In this work, we look at a recurrent formulation of neural networks (RNNs) that are deep in time and can store state information from an arbitrarily large input history when making a prediction. We show that RNNs provide improved performance over DNNs of comparable size in terms of various objective metrics for a variety of prosodic streams (notably, a relative reduction of about 6% in F0 mean-square error accompanied by a relative increase of about 14% in F0 variance), as well as in terms of perceptual quality assessed through mean-opinion-score listening tests. Index Terms: speech synthesis,text-to-speech, prosody prediction, recurrent neural networks, deep learning
Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq

Cellular diversity in the brain revealed The mammalian brain has an extraordinarily large number of cells. Although there are quite a few different cell types, many cells in any one category tend to look alike. Zeisel et al. analyzed the transcriptomes of mouse brain cells to reveal more than meets the eye. Interneurons of similar type were found in dissimilar regions of the brain. Oligodendrocytes that seemed to be all of one class were differentiated by their molecular signatures into a half-dozen classes. Microglia associated with blood vessels were distinguished from look-alike perivascular macrophages. Thus, the complex microanatomy of the brain can be revealed by the RNAs expressed in its cells. Science, this issue p. 1138 A close look at the genes expressed by cells in the brain reveals hidden and coordinated cellular complexity. The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviors. Normal brain function relies on a diverse set of differentiated cell types, including neurons, glia, and vasculature. Here, we have used large-scale single-cell RNA sequencing (RNA-seq) to classify cells in the mouse somatosensory cortex and hippocampal CA1 region. We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex. We identified numerous marker genes, which allowed alignment with known cell types, morphology, and location. We found a layer I interneuron expressing Pax6 and a distinct postmitotic oligodendrocyte subclass marked by Itpr2. Across the diversity of cortical cell types, transcription factors formed a complex, layered regulatory code, suggesting a mechanism for the maintenance of adult cell type identity.
IKKα inactivation promotes Kras-initiated lung adenocarcinoma development through disrupting major redox regulatory pathways

Significance Reactive oxygen species (ROS) can promote tumorigenesis or kill cancer cells. How different cancer-associated genetic alterations regulate ROS balance and outcome is of great importance for the design of rational cancer treatments, many of which affect ROS metabolism and sensing. Kras activation induces a ROS defense system and cell senescence, which counteract its oncogenic activity. KRAS-activating mutations are accompanied by IKKα loss mutations that result in elevated NOX2 but decreased expression of the NRF2 ROS defense system. Thus, IKKα ablation turns the antitumorigenic effect of Kras-induced ROS to a protumorigenic effect that enhances Kras-induced progression of lung adenocarcinoma (ADC). Restoration of IKKα activity or inhibition of the pathways activated on its loss may offer new opportunities for ADC treatment. Lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC) are two distinct and predominant types of human lung cancer. IκB kinase α (IKKα) has been shown to suppress lung SCC development, but its role in ADC is unknown. We found inactivating mutations and homologous or hemizygous deletions in the CHUK locus, which encodes IKKα, in human lung ADCs. The CHUK deletions significantly reduced the survival time of patients with lung ADCs harboring KRAS mutations. In mice, lung-specific Ikkα ablation (IkkαΔLu) induces spontaneous ADCs and promotes KrasG12D-initiated ADC development, accompanied by increased cell proliferation, decreased cell senescence, and reactive oxygen species (ROS) accumulation. IKKα deletion up-regulates NOX2 and down-regulates NRF2, leading to ROS accumulation and blockade of cell senescence induction, which together accelerate ADC development. Pharmacologic inhibition of NADPH oxidase or ROS impairs KrasG12D-mediated ADC development in IkkαΔLu mice. Therefore, IKKα modulates lung ADC development by controlling redox regulatory pathways. This study demonstrates that IKKα functions as a suppressor of lung ADC in human and mice through a unique mechanism that regulates tumor cell-associated ROS metabolism.
Should Perineal Descent Be Managed as a Multifactorial Clinicopathologic Entity in Patients With Obstructed Defecation?

To the Editor—transverse perineal support is a surgical procedure that aims to create a support element to the pelvic floor in patients with obstructed defecation syndrome (oDs) and perineal descent (PD). our data, reported for the first time in the Journal, show that transverse perineal support has been usefully adopted to correct pathological PD in patients with oDs. the rationale of the transverse perineal support is that, in patients with a pathological PD, the Valsalva maneuver, because of the weakness of the perineum, can only determine a greater depth of the pelvis without achieving a reduction of the abdominal volume. thus, the expected increase of the intra-abdominal pressure, indispensable to the defecation, is not reached. Given these considerations, we are in agreement with the authors of comments regarding the fact that the effectiveness of the Valsalva maneuver is not only related to functional integrity of the perineum but also requires a synergic function of thoracic and abdominal muscles. hence, as suggested by Brusciano et al, the idea of being able to identify and rehabilitate the subgroup experiencing these thoraco-abdominal disorders among the oDs group of patients is certainly intriguing and potentially useful. the key question, however, remains the same: what we can do to treat a full-blown PD in patients with oDs? PD can be observed in 75% to 84% of patients with Should Perineal Descent Be Managed as a Multifactorial Clinicopathologic Entity in Patients With Obstructed Defecation?
cAMP signal transduction in the heart: understanding spatial control for the development of novel therapeutic strategies

3′‐5′‐cyclic adenosine monophosphate (cAMP) is a pleiotropic intracellular second messenger generated in response to activation of Gs protein‐coupled receptors. In the heart, cAMP mediates the catecholaminergic control on heart rate and contractility but, at the same time, it is responsible for the functional response to a wide variety of other hormones and neurotransmitters, raising the question of how the myocyte can decode the cAMP signal and generate the appropriate functional output to each individual extracellular stimulus. A growing body of evidence points to the spatial organization of the components of the cAMP signalling pathway in distinct, spatially segregated signalling domains as the key feature underpinning specificity of response and data is emerging, indicating that alteration of spatial control of the cAMP signal cascade associates with heart pathology. Most of the details of the molecular organization and regulation of individual cAMP signalling compartments are still to be elucidated but future research should provide the knowledge necessary to develop and test new therapeutic strategies that, by acting on a limited subset of downstream targets, would improve efficacy and minimize off‐target effects.
Detection and identification of the two Candidatus Liberobacter species associated with citrus huanglongbing by PCR amplification of ribosomal protein genes of the beta operon.

Huanglongbing, previously called greening, is a destructive disease of citrus in Asia and Africa. It is caused by an uncultured, phloem-restricted bacterium for which two species Candidatus Liberobacter asiaticum and Candidatus Liberobacter africanum have been characterized. In 1996, a polymerase chain reaction (PCR) detection method based on the amplification of the 16S ribosomal operon was developed and proved to be efficient for the detection of both liberobacter species. However, in order to distinguish between Candidatus Liberobacter asiaticum and Candidatus Liberobacter africanum, digestion of the amplified DNA with XbaI was required. We have now developed a new PCR detection method based on the amplification of ribosomal protein genes which allows direct identification of the liberobacter species by the size of the amplified DNA. This PCR method is as specific, and at least as sensitive as the previously described one for detection of the two liberobacter species
Dating deformation using crushed alkali feldspar : 1 40 Ar / 39 Ar geochronology of shear zones in the Wyangala

Diffusion parameters have been estimated for K6 feldspar in and adjacent to mylonite shear zones in the 7 Wyangala Batholith. The parameters obtained suggest that 8 deformation during mylonitization would have caused argon 9 systematics to reset because diffusion distances were reduced 10 by cataclasis, deformation and/or recrystallization. However, the 11 mineral lattice remained sufficiently retentive to allow 12 subsequently produced radiogenic argon to be retained. 13 40Ar/39Ar geochronology is thus able to constrain operation of 14 these biotite-grade ductile shear zones to the period from ~380 15 Ma to ~360 Ma, at the end of the Tabberabberan Orogeny. 16
Prevalence of Orofacial Clefts in Nigeria

Orofacial clefts are the most common malformations of the head and neck. In Africa, orofacial clefts are underascertained, with little or no surveillance system in most parts for clefts and other birth defects. A Nigerian craniofacial anomalies study, NigeriaCRAN, was established in 2006 to support cleft research specifically for epidemiological studies, treatment outcomes, and studies into etiology and prevention. We pooled data from seven of the largest Smile Train treatment centers in the six geopolitical zones in Nigeria. Data from September 2006 to June 2011 were analyzed and clefts compared between sides and genders using the Fisher exact test. A total of 2197 cases were identified during the study period, with an estimated prevalence rate of 0.5 per 1000. Of the total number of orofacial clefts, 54.4% occur in males and 45.6% in females. There was a significant difference (P=.0001) between unilateral left clefts and unilateral right clefts, and there was a significant difference (P=.0001) between bilateral clefts and clefts on either the left or right side. A significant gender difference (P=.03) was also observed for cleft palate, with more females than males. A total of 103 (4.7%) associated anomalies were identified. There were nine syndromic cleft cases, and 10.4% of the total number of individuals with clefts have an affected relative. The significant difference between unilateral clefts and the gender differences in the proportion of cleft palate only are consistent with the literature. The present study emphasizes the need for birth defects registries in developing countries in order to estimate the exact prevalence of birth defects including orofacial clefts.
The pros and cons of the inversion method approach to derive 3D dust emission properties in the ISM: the Hi-GAL field centred on (l, b) = (30°, 0°)

Herschel far-infrared continuum data obtained as part of the Hi-GAL survey have been used, together with the GLIMPSE 8 μm and MIPSGAL 24 μm data, to attempt the first 3D-decomposition of dust emission associated with atomic, molecular and ionized gas at 15 arcmin angular resolution. Our initial test case is a 2 × 2 square degrees region centred on (l, b) = (30°, 0°), a direction that encompasses the origin point of the Scutum–Crux Arm at the tip of the Galactic Bar. Coupling the IR maps with velocity maps specific for different gas phases (H i 21cm, ^(12)CO and ^(13)CO, and radio recombination lines), we estimate the properties of dust blended with each of the gas components and at different Galactocentric distances along the line of sight (LOS). A statistical Pearson's coefficients analysis is used to study the correlation between the column densities estimated for each gas component and the intensity of the IR emission. This analysis provides evidence that the 2 × 2 square degree field under consideration is characterized by the presence of a gas component not accounted for by the standard tracers, possibly associated with warm H_2 and cold H I. We demonstrate that the IR radiation in the range 8 < λ < 500 μm is systematically dominated by emission originating within the Scutum–Crux Arm. By applying an inversion method, we recover the dust emissivities associated with atomic, molecular and ionized gas. Using the DustEM model, we fit the spectral energy distributions for each gas phase, and find average dust temperatures of T_(d,H I) = 18.82 ± 0.47 K, T_(d,H_2) = 18.84 ± 1.06 K and T_(d,H II) = 22.56 ± 0.64 K, respectively. We also obtain an indication for polycyclic aromatic hydrocarbons depletion in the diffuse ionized gas. We demonstrate the importance of including the ionized component in 3D-decompositions of the total IR emission. However, the main goal of this work is to discuss the impact of the missing column density associated with the dark gas component on the accurate evaluation of the dust properties, and to shed light on the limitations of the inversion method approach when this is applied to a small section of the Galactic plane and when the working resolution allows sufficient de-blending of the gas components along the LOS.
Assessing language dominance in Mandarin–English bilinguals: Convergence and divergence between subjective and objective measures*

This study examines the convergence and divergence between subjective and objective measures of language proficiency for assessing language dominance in Mandarin–English bilinguals. Sixty-two young adults (Experiment 1) and 27 children (Experiment 2) provided self-ratings of proficiency level (or were rated by their parents), were interviewed for spoken proficiency, and named pictures in the Multilingual Naming Test (MINT) and (in Experiment 1 only) the Boston Naming Test. In Experiment 1, the four measures converged in the number of people classified into different dominance groups but both naming tests indicated greater English dominance than self-report and interview measures. In Experiment 2, parent report and interview measures converged in dominance classifications but the MINT indicated higher degrees of English dominance. To a large extent bilinguals were able to classify themselves (or their children) into dominance groups but some mismatches between measures in dominance classification were observed for all age and dominance groups. These results, together with previous findings with Spanish–English bilingual adults (Gollan et al., 2012), suggest that bilinguals may shift to English dominance in confrontation naming before they do so in conversational fluency, and that dominance shifts persist throughout the lifespan but may be relatively more pronounced in children. These findings caution against the use of self-reports as the sole means of classifying bilinguals into dominance groups and support a multi-measure approach including direct assessment of the relevant linguistic domain.