Miracle-dz/UniFAR-FTUs huggingface dataset:
Variability in Clinical Outcomes for Youths Treated for Subthreshold Severe Mental Disorders at an Early Intervention Service.

OBJECTIVE The study assessed the longitudinal clinical and functional outcomes of young people receiving early intervention services for subthreshold manifestations of severe mental disorders at a youth mental health service in Sydney, Australia. METHODS The six-month, prospective, longitudinal study collected data on clinical outcomes of 243 young people treated for subsyndromal presentations of putative major mental disorders. Reliable change index scores and effect sizes were calculated to compare results at baseline, three months, and six months for measures of symptoms (Brief Psychiatric Rating Scale [BPRS]), psychological distress (Kessler 10 [K10]), and social functioning (Social and Occupational Functioning Assessment Scale [SOFAS]). RESULTS There was variability in outcomes across each of the outcome measures, with 25%, 33%, and 23% of patients showing reliable improvement and 9%, 13%, and 5% showing reliable deterioration for the SOFAS, K10, and BPRS, respectively. Many individuals did not show linear improvement or deterioration, with reliable change within individuals varying significantly between the zero- to three-month and three- to six-month time points. After the analyses were controlled for covariates, baseline severity or impairment and number of sessions with a psychiatrist or psychologist contributed a small (4%-8%) but significant amount to the total variance in outcomes at six months. CONCLUSIONS Most individuals did not show significant deterioration, and about one in four showed reliable improvement by six months. However, individual patterns of change were diverse, highlighting the importance of further research into factors that influence treatment outcomes among youths with subthreshold presentations of severe mental disorders.
Fabrication of silver nanoparticles using Arnebia hispidissima (Lehm.) A. DC. root extract and unravelling their potential biomedical applications

Abstract The present study reports the biosynthesis of silver nanoparticles using aqueous root extract of Arnebia hispidissima. They were prepared by adding 10 mL root extract in 90 mL silver nitrate (0.5 mM) solution and heating at 60 ± 2 °C for 12 min at pH 7.5. Characterization of the biosynthesized silver nanoparticles was done using UV-Visible spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, transmission electron microscopy, X-ray diffraction analysis, dynamic light scattering measurements and Fourier-transform infrared spectroscopy. The synthesized silver nanoparticles were crystalline in nature exhibiting different shapes like sphere, rod, triangle, hexagon and polygon. Their zeta potential was −23.6 mV confirming their high stability. Fourier-transform infrared spectroscopy analysis showed the presence of phenolics, flavonoids and proteins as reducing, capping and stabilizing agents. The synthesized nanoparticles showed effective in vitro anti-oxidant activity against DPPH (IC50 = 9.86 µg/mL) and H2O2 (IC50 = 53.78 µg/mL) radicals. The nanoparticles showed dose-dependent cytotoxicity against HeLa (cervical cancer, IC50 = 4.44 µg/mL) cells and were non-toxic towards normal L20 B cells (non-malignant mouse cell line). They also exhibited strong anti-microbial activity against Candida albicans, Candida tropicalis, Geotrichum candidum (fungal strains); Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae (bacterial strains). This is the first report of synthesis of silver nanoparticles using A. hispidissima root extract validating their bioefficacy against HeLa cancer cells and diverse microorganisms.
A Functional Unfolded Protein Response Is Required for Normal Vegetative Development1[OPEN]

Although the unfolded protein response is known as a stress response in plants, it also functions in normal plant development. The unfolded protein response (UPR) is activated in plants in response to endoplasmic reticulum stress and plays an important role in mitigating stress damage. Multiple factors act in the UPR, including the membrane-associated transcription factor, BASIC LEUCINE ZIPPER 17 (bZIP17), and the membrane-associated RNA splicing factor, INOSITOL REQUIRING ENZYME1 (IRE1). We have analyzed an Arabidopsis (Arabidopsis thaliana) ire1a ire1b bzip17 triple mutant, with defects in stress signaling, and found that the mutant is also impaired in vegetative plant growth under conditions without externally applied stress. This raised the possibility that the UPR functions in plant development in the same manner as it does in responding to stress. bZIP17 is mobilized to the nucleus in response to stress, and through the analysis of a mobilization-defective bZIP17 mutant, we found that to support normal plant development bZIP17 must be capable of mobilization. Likewise, through the analysis of ire1 mutants defective in either protein kinase or RNase activities, we found that both must be operative to promote normal development. These findings demonstrate that the UPR, which is associated with stress responses in plants, also functions under unstressed conditions to support normal development.
Structures of ATP-bound DNA ligase D in a closed domain conformation reveal a network of amino acid and metal contacts to the ATP phosphates

DNA ligases are the sine qua non of genome integrity and essential for DNA replication and repair in all organisms. DNA ligases join 3′-OH and 5′-PO4 ends via a series of three nucleotidyl transfer steps. In step 1, ligase reacts with ATP or NAD+ to form a covalent ligase-(lysyl-Nζ)–AMP intermediate and release pyrophosphate (PPi) or nicotinamide mononucleotide. In step 2, AMP is transferred from ligase-adenylate to the 5′-PO4 DNA end to form a DNA-adenylate intermediate (AppDNA). In step 3, ligase catalyzes attack by a DNA 3′-OH on the DNA-adenylate to seal the two ends via a phosphodiester bond and release AMP. Eukaryal, archaeal, and many bacterial and viral DNA ligases are ATP-dependent. The catalytic core of ATP-dependent DNA ligases consists of an N-terminal nucleotidyltransferase domain fused to a C-terminal OB domain. Here we report crystal structures at 1.4–1.8 Å resolution of Mycobacterium tuberculosis LigD, an ATP-dependent DNA ligase dedicated to nonhomologous end joining, in complexes with ATP that highlight large movements of the OB domain (∼50 Å), from a closed conformation in the ATP complex to an open conformation in the covalent ligase-AMP intermediate. The LigD·ATP structures revealed a network of amino acid contacts to the ATP phosphates that stabilize the transition state and orient the PPi leaving group. A complex with ATP and magnesium suggested a two-metal mechanism of lysine adenylylation driven by a catalytic Mg2+ that engages the ATP α phosphate and a second metal that bridges the ATP β and γ phosphates.
Path analysis for key factors influencing long-term quality of life of patients following a percutaneous coronary intervention

Supplemental Digital Content is available in the text. Objectives This cross-sectional study aimed to investigate the long-term quality of life (QOL) influencing of patients following a percutaneous coronary intervention (PCI) as well as its influencing factors. Patients and methods From June 2013 to April 2014, 428 PCI patients were enrolled in this questionnaire survey. The demographic and clinical data, Social Support Rating Scale, Medical Coping Modes Questionnaire, Social Disability Screening Schedule, and Short Form 36 Health Status Questionnaire were collected. Statistical analyses for data and path analyses for influencing factors were then carried out. Results PCI patients received considerable social support from family and society, and most PCI patients adopted negative coping styles (avoidance and acceptance-resignation). Approximately 70.3% of PCI patients had a serious functional defect, and 96.97% of patients had an average (79.91%) or better (17.06%) QOL. Multiple linear regression analysis showed that long-term QOL of PCI patients was correlated positively with social support and sleep quality, but correlated negatively with the acceptance-resignation coping style, social function defects, and number of adverse cardiac events. Path analysis further showed that social support, acceptance-resignation coping style, social function defects, number of adverse cardiac events, and sleep quality exerted important effects on long-term QOL of PCI patients in descending order. Conclusion Most PCI patients had an average medium-term or better long-term QOL. Social support, acceptance-resignation coping style, social function defects, number of adverse cardiac events, and sleep quality were key influencing factors.
A Cdc42 GEF, Gef1, through endocytosis organizes F-BAR Cdc15 along the actomyosin ring and promotes concentric furrowing

ABSTRACT During cytokinesis, fission yeast coordinates actomyosin ring constriction with septum ingression, resulting in concentric furrow formation by a poorly defined mechanism. We report that Schizosaccharomyces pombe cells lacking the Cdc42 activator Gef1, combined with an activated allele of the formin, Cdc12, display non-concentric furrowing. Non-concentrically furrowing cells display uneven distribution of the scaffold Cdc15 along the ring. This suggests that, after ring assembly, uniform Cdc15 distribution along the ring enables proper furrow formation. We find that, after assembly, Cdc15 is recruited to the ring in an Arp2/3 complex-dependent manner and is decreased in the activated cdc12 mutant. Cdc15 at cortical endocytic patches shows increased levels and extended lifetimes in gef1 and activated cdc12 mutants. We hypothesize endocytosis helps recruit Cdc15 to assembled rings; uneven Cdc15 distribution at the ring occurs when endocytic patches contain increased Cdc15 levels and the patch-association rate is slow. Based on this, we developed a mathematical model that captures experimentally observed Cdc15 distributions along the ring. We propose that, at the ring, Gef1 and endocytic events promote uniform Cdc15 organization to enable proper septum ingression and concentric furrow formation. Highlighted Article: Gef1 and endocytic events at the assembled actomyosin ring facilitate uniform F-BAR protein Cdc15 distribution along the ring thus enabling concentric furrow formation.
Safety of direct oral anticoagulants in real–world clinical practice: translating the trials to everyday clinical management

ABSTRACT Introduction: Direct oral anticoagulants (DOACs) may be regarded as some of the most successful innovations in recent times. These drugs which were specifically developed to overcome the challenges posed by warfarin did just that and in the process, have changed the outlook towards stroke prevention with anticoagulation. The decade of experience with these drugs that has resulted in the availability of large scale data on their safety profile has aided this. Areas covered: This review examines existing real-world studies (RWS) and their interpretation to better appreciate how they either complement or contradict findings from the hallmark trials. Specific focus has been made on the safety of DOACs, on their risks of major bleeding, intra-cranial haemorrhage (ICH), gastro-intestinal (GI) bleeding and all-cause mortality compared to warfarin and each other. DOAC use in the elderly and other sub-groups are briefly discussed. Expert opinion: Results for safety outcomes according to ‘real world evidence’ (RWE) are in-keeping with randomised controlled trials (RCTs) and currently, all 4 DOACs have been deemed at least as effective as warfarin, while demonstrating superiority in some aspects. While real world studies act as a complementary source of knowledge, traditional RCTs remain the gold standard for determining cause-effect relationships.
Influence of hatch time and access to feed on intramuscular adipose tissue deposition in broilers.

The effect of hatch time and subsequent access to feed on intramuscular adipose tissue deposition was studied in the pectoralis major muscle of male Ross 308 broiler chickens. Based on their hatch time chicks were classified as early (EH), midterm (MH), or late (LH) hatchers, with an average incubation duration of 497.7 h for EH, 508.8 h for MH, and 514.5 h for LH birds. Chicks were provided access to feed either immediately at hatch, or 24 h after the conclusion of the hatch window. Expression of the adipogenic regulatory genes peroxisome proliferator-activated receptor gamma (PPARγ), and stearoyl-CoA desaturase (SCD), were measured at the time of hatch, and zero, one, 4, 7, 28, and 40 d. Intramuscular adipocyte cell width and visualization of adipose tissue deposition was observed at 28 and 40 d. Expression of PPARγ was increased in the pectoralis major of LH birds at the time of hatch, zero, and one d. The expression of PPARγ at one and 7 d, and SCD at 7 d were increased in all birds that received delayed access to feed. At 28 d, adipocyte cell width was increased in LH birds with delayed access to feed, compared to EH and MH birds with delayed access to feed and LH birds with immediate access to feed. At 40 d, adipocyte cell width was increased in all birds that received delayed access to feed. Also at 40 d, there was a trend (P = 0.078) for more extensive intramuscular adipose tissue deposition in LH than EH birds, and in birds with delayed access to feed (P = 0.075). These data indicate delayed access to feed increases intramuscular adipose tissue deposition in the pectoralis major muscle, and suggest that hatch time influences this regulation.
The First Decade of Web-Based Sports Injury Surveillance: Descriptive Epidemiology of Injuries in US High School Girls' Basketball (2005-2006 Through 2013-2014) and National Collegiate Athletic Association Women's Basketball (2004-2005 Through 2013-2014).

CONTEXT The advent of Web-based sports injury surveillance via programs such as the High School Reporting Information Online system and the National Collegiate Athletic Association Injury Surveillance Program has aided the acquisition of girls' and women's basketball injury data. OBJECTIVE To describe the epidemiology of injuries sustained in high school girls' basketball in the 2005-2006 through 2013-2014 academic years and collegiate women's basketball in the 2004-2005 through 2013-2014 academic years using Web-based sports injury surveillance. DESIGN Descriptive epidemiology study. SETTING Online injury surveillance from basketball teams in high school girls (annual average = 100) and collegiate women (annual average = 57). PATIENTS OR OTHER PARTICIPANTS Girls' and women's basketball players who participated in practices and competitions during the 2005-2006 through 2013-2014 academic years in high school or the 2004-2005 through 2013-2014 academic years in college. MAIN OUTCOME MEASURE(S) Certified athletic trainers collected time-loss (≥24 hours) injury and exposure data. Injury rates per 1000 athlete-exposures (AEs) were calculated. Injury rate ratios (IRRs) with 95% confidence intervals (CIs) were used to compare injury rates by school size or division, time in season, event type, and competition level. RESULTS The High School Reporting Information Online system documented 2930 time-loss injuries during 1 609 733 AEs; the National Collegiate Athletic Association Injury Surveillance Program documented 3887 time-loss injuries during 783 600 AEs. The injury rate was higher in college than in high school (4.96 versus 1.82/1000 AEs; IRR = 2.73; 95% CI = 2.60, 2.86). The injury rate was higher in competitions than in practices for both high school (IRR = 3.03; 95% CI = 2.82, 3.26) and collegiate (IRR = 1.99; 95% CI = 1.86, 2.12) players. The most common injuries at both levels were ligament sprains, concussions, and muscle/tendon strains; the majority of injuries affected the ankle, knee, and head/face. These injuries were often caused by contact with another player or a noncontact mechanism. CONCLUSIONS Injury rates were higher in collegiate than in high school athletes and in competitions than in practices. Similarities in distributions of injuries by body parts, specific diagnoses, and mechanisms of injury suggest that both levels may benefit from similar injury-prevention strategies.
Somatic Mutation Is a Function of Clone Size and Depth in Orbicella Reef-Building Corals

In modular organisms, the propagation of genetic variability within a clonal unit can alter the scale at which ecological and evolutionary processes operate. Genetic variation within an individual primarily arises through the accretion of somatic mutations over time, leading to genetic mosaicism. Here, we assess the prevalence of intraorganismal genetic variation and potential mechanisms influencing the degree of genetic mosaicism in the reef corals Orbicella franksi and Orbicella annularis. Colonies of both species, encompassing a range of coral sizes and depths, were sampled multiple times and genotyped at the same microsatellite loci to detect intraorganismal genetic variation. Genetic mosaicism was detected in 38% of corals evaluated, and mutation frequency was found to be positively related with clonal size and negatively associated with coral depth. We suggest that larger clones experience a greater number of somatic cell divisions and consequently have an elevated potential to accumulate mutations. Furthermore, corals at shallower depths may be exposed to abiotic conditions such as elevated thermal regimes, which promote increased mutation rates. The results highlight the pervasiveness of intraorganismal genetic variation in reef-building corals and emphasize potential mechanisms generating somatic mutations in modular organisms.
Mitogenomes of genus Pristipomoides, Lutjanus and Pterocaesio confirm Caesionidae nests in Lutjanidae

Abstract In this study, the complete mitochondrial genome of Goldbanded jobfish (Perciformes: Lutjanidae: Etelinae: Pristipomoides multidens) was sequenced using long-PCR amplification (Accession No.: KF430626). The mitochondrial genome is highly homologous to those of other teleosts. Consensus tree based on phylogeny analysis of nucleotide sequences using the protein-coding regions combined sequences (apart from ND6) in 11 species supports the fact that Pristipomoides multidens of Etelinae and the cluster of Subfamily Lutjaninae remain the sister relationship, consistent with the traditional taxonomy, while the fusiliers (Pterocaesio tile) of family Caesionidae nested within the genus Lutjanus of family Lutjanidae and showed more closely relationship with the non-reddish snappers cluster including L. russellii and L. argentimaculatus. Conclusively, this mitogenomic phylogeny indicates that the Caesionidae is a synonym of family Lutjanidae. Given the morphologic and feeding diversity, we hypothesize that the variation of feeding systems of genus Lutjanus may drive the speciation of snappers and fusiliers.
Dynamic Saliency-Aware Regularization for Correlation Filter-Based Object Tracking

With a good balance between tracking accuracy and speed, correlation filter (CF) has become one of the best object tracking frameworks, based on which many successful trackers have been developed. Recently, spatially regularized CF tracking (SRDCF) has been developed to remedy the annoying boundary effects of CF tracking, thus further boosting the tracking performance. However, SRDCF uses a fixed spatial regularization map constructed from a loose bounding box and its performance inevitably degrades when the target or background show significant variations, such as object deformation or occlusion. To address this problem, we propose a new dynamic saliency-aware regularized CF tracking (DSAR-CF) scheme. In DSAR-CF, a simple yet effective energy function, which reflects the object saliency and tracking reliability in the spatial–temporal domain, is defined to guide the online updating of the regularization weight map using an efficient level-set algorithm. Extensive experiments validate that the proposed DSAR-CF leads to better performance in terms of accuracy and speed than the original SRDCF.
Association of Nutritional Factors with Hearing Loss

Hearing loss (HL) is a major public health problem. Nutritional factors can affect a variety of diseases, such as HL, in humans. Thus far, several studies have evaluated the association between nutrition and hearing. These studies found that the incidence of HL was increased with the lack of single micro-nutrients such as vitamins A, B, C, D and E, and zinc, magnesium, selenium, iron and iodine. Higher carbohydrate, fat, and cholesterol intake, or lower protein intake, by individuals corresponded to poorer hearing status. However, higher consumption of polyunsaturated fatty acids corresponded to better hearing status of studied subjects. In addition to malnutrition, obesity was reported as a risk factor for HL. In studies of the relationship between middle ear infection and nutrition in children, it was reported that lack of vitamins A, C and E, and zinc and iron, resulted in poorer healing status due to vulnerability to infection. These studies indicate that various nutritional factors can affect hearing. Therefore, considering that multifactorial nutritional causes are responsible, in part, for HL, provision of proper guidelines for maintaining a proper nutritional status is expected to prevent some of the causes and burden of HL.
An audit and service evaluation of the use of cone beam computed tomography (CBCT) in a paediatric dentistry department.

OBJECTIVES CBCT exposes the paediatric patient to a higher X-ray dose and risk than normal dental radiographs. This study has two components: an audit and service evaluation. The audit aims to assess whether the use of CBCT in a Paediatric Dentistry department at a London hospital complies with European guidelines (SEDENTEXCT). The service evaluation aims to explore the influence of CBCT on treatment planning. METHODS Two 6 month audit cycles were completed, where CBCT requests were audited to check whether image justifications comply with SEDENTEXCT. For the service evaluation, a total of 50 patient records were examined for the effect of CBCT on definitive treatment plans. RESULTS The first audit demonstrated 94% compliance with SEDENTEXCT. After instituting staff training in CBCT, compliance improved to 100%. In the service evaluation, 100% of CBCTs were found to provide information that impacted on the clinicians' treatment planning, diagnosis and/or management. Of most significance, 44% of treatment plans were changed because of new information provided by CBCT. CONCLUSIONS There are few studies investigating the use of CBCT in paediatric dentistry and the impact of this investigation. This service evaluation shows that CBCT can play an important role in optimising paediatric patient outcomes. The need for robust staff training in CBCT referrals to prevent over prescription is demonstrated in the audit cycles.