Miracle-dz/UniFAR-FTUs huggingface dataset:
Hypervitaminosis A in experimental nonhuman primates: evidence, causes, and the road to recovery

One of the great underlying assumptions made by all scientists utilizing primate models for their research is that the optimal nutritional status and health of the animals in use has been achieved. That is, no nutrient deficiency or excess has compromised their health in any detectable way. To meet this assumption, we rely on the National Research Council's (NRC's) nutritional recommendations for nonhuman primates to provide accurate guidance for proper dietary formulations. We also rely on feed manufacturers to follow these guidelines. With that in mind, the purpose of this commentary is to discuss three related points that we believe have significant ramifications for the health and well being of captive primates as well as for their effective use in biomedical research. First, our laboratory has shown that most experimental primates are likely in a state of hypervitaminosis A. Second, it is apparent that many primate diets are providing vitamin A at levels higher than the NRC's recommendation. Third, the recommendation itself is based on inadequate information about nutrient needs and is likely too high, especially when compared with human requirements. Am. J. Primatol. 71:813–816, 2009. © 2009 Wiley‐Liss, Inc.
The Clinical Impact of Islet Transplantation

Islet cell transplantation has recently emerged as one of the most promising therapeutic approaches to improving glycometabolic control in diabetic patients and, in many cases, achieving insulin independence. Unfortunately, many persistent flaws still prevent islet transplantation from becoming the gold standard treatment for type 1 diabetic patients. We review the state of the art of islet transplantation, outcomes, immunosuppression and—most important—the impact on patients' survival and long‐term diabetic complications and eventual alternative options. Finally, we review the many problems in the field and the challenges to islet survival after transplantation. The rate of insulin independence 1 year after islet cell transplantation has significantly improved in recent years (60% at 1 year posttransplantation compared with 15% previously). Recent data indicate that restoration of insulin secretion after islet cell transplantation is associated with an improvement in quality of life, with a reduction in hypoglycemic episodes and potentially with a reduction in long‐term diabetic complications. Once clinical islet transplantation has been successfully established, this treatment could even be offered to diabetic patients long before the onset of diabetic complications.
A randomized experiment to examine unintended consequences of dietary supplement use among daily smokers: taking supplements reduces self-regulation of smoking.

This letter is a reaction to the paper by Chiou and others, in the December 2011 issue of Addiction, on how the use of dietary supplements may induce illusory invulnerability that, in turn, disinhibit smoking [1]. The findings run counter to other research findings of large population samples regarding healthy behaviors of individuals who consume dietary supplements regularly. Recent analysis of the US National Health and Nutrition Examination Survey (NHANES) data finds that dietary supplement use is associated with the avoidance of smoking among American consumers [2,3]. NHANES is a large government program designed to assess the health and nutritional status of adults and children in the United States. Furthermore, the Healthy Habits of Supplement Users and Non-Users survey found that a larger proportion of non-users smoke cigarettes (26% versus 15%, respectively) [4]. Even if there is a potential ‘licensing’ effect that causes a small portion of dietary supplement users to smoke more, the large body of evidence suggests that this does not trump the overall healthier habits of supplement users, including full avoidance from smoking. This leads to the question that if individuals in the study took dietary supplements continuously (not just a placebo), would they be more prone to stop smoking and live healthier life-styles? The outcomes of the study may not be generalized, as the prevalence and attitudes towards smoking are different in this subpopulation of undergraduate Taiwanese students. The study may have also induced bias among the participants, as it was not a blind study (the undergraduate students knew which treatment group they were in as well, as the proposed hypothesis of the study). The authors of this study should consider monitoring the long-term benefits of actual dietary supplement use in university students as a mechanism to help them develop healthier life-style habits.
Impaired glucose tolerance, diabetes, and cardiovascular disease.

OBJECTIVE To analyze the association among impaired glucose tolerance (IGT), diabetes, and cardiovascular disease (CVD). METHODS We review current studies that have addressed the foregoing relationship and summarize the results of diagnostic and therapeutic interventions. RESULTS A major cause of the reduction in life expectancy in patients with diabetes is CVD and cardiovascular complications. Both prediabetes and diabetes predispose to cardiovascular alterations. IGT and even the upper normal values of nondiabetic glucose levels are associated with an increased cardiovascular risk. The risk of heart disease can be increased as early as 15 years before the diagnosis of diabetes. Patients with chronic or acute CVD and no previous diagnosis of diabetes frequently present with either IGT or diabetes. Thus, such patients should undergo screening for diabetes with an oral glucose tolerance test. In acute coronary syndromes, lowering of glucose levels to the near-normal range by administration of insulin is highly beneficial. Early, rigorous interventions to improve metabolic control will yield better cardiovascular outcomes in patients with dysglycemia. CONCLUSION Aggressive preventive and treatment strategies, which can include multiple interventions, are needed to minimize the potential effects of CVD in patients with diabetes or IGT.
Genital warts-a venereal disease.

The number of cases of genital warts (condyloma accuminata, verruca accuminata) has been noted to increase tremendously on the establishment of a replacement center at Camp Atterbury, Indiana. After treating approximately 50 cases of these lesions and seeing the high incidence in the troops returning from overseas compared to civilians or soldiers from the United States, we have felt that a closer evaluation should be made. After a study of 70 men and 24 women, we are making a preliminary report because we feel the medical profession should be alerted to this problem for the reasons to be discussed. TREATMENT IN MEN Complete studies of 70 men have been carried out (fig. 1). Of these patients, 65 had been stationed in Korea and Japan, 4 had never been out of the United States, and one was in Okinawa. All 65 patients who were in the Far East admitted contact with
Endoscopic ultrasound-guided fine needle aspiration: The wet suction technique

Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) has become a fundamental tool in obtaining cytopathological diagnosis of pancreatic tumors. When sampling solid lesions of the pancreas, the endosonographer can use two suction techniques to enhance tissue acquisition; the dry and the wet suction techniques. The standard dry suction technique relies on applying negative pressure suction on the proximal end of the needle after the stylet is removed with a pre-vacuum syringe. The wet suction technique relies on pre-flushing the needle with saline to replace the column of air with fluid followed by aspiration the proximal end by using a prefilled syringe with saline. A new modified wet suction technique (hybrid suction technique) relies on preloading the needle with saline, but having continuous negative pressure with a pre-vacuum syringe to avoid manual intermittent suction. Tissue acquisition can be enhanced by applying fluid dynamic principles to the current aspiration techniques, such as the column of water used in the needle of the wet technique. In this review, we will focus on EUS-FNA using the wet suction technique for sampling of pancreatic solid lesions.
Two Independent Regions of Human Telomerase Reverse Transcriptase Are Important for Its Oligomerization and Telomerase Activity*

Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, contains motifs conserved among reverse transcriptases. Several nucleic acid-dependent polymerases that share a “fingers, palm, and thumb substructure” were shown to oligomerize. Here we demonstrate that hTERT also has this ability using partially purified recombinant hTERTs and mammalian cells co-expressing differently tagged hTERTs. Human template RNA (hTR), by contrast, has no effect on the structural oligomerization of hTERTs. Therefore, hTERT has an intrinsic ability of oligomerization in the absence of hTR. We identified two separate regions as essential for the oligomerization. The regions, amino acids 301–538 (amino-terminal region) and amino acids 914–928 (carboxyl-terminal region), are outside the fingers and palm substructure covering motif T to D and interact with each otherin vivo. A substituted mutant of hTERT, hTERT-D712A-V713I, which was reported as a dominant negative form of hTERT, bound to the wild-type hTERT and inhibited its telomerase activity transiently expressed in telomerase-negative finite normal human fibroblast. The truncated forms of hTERT containing the binding region to the wild-type hTERT partially inhibited the telomerase activity, probably by preventing the wild-type hTERT from forming an oligomer. Taken together, the oligomerization of hTERT is an important step for telomerase activity.
Vascular response to overlapping everolimus-eluting stents. - Comparison with paclitaxel-eluting stents -.

BACKGROUND Overlapping drug-eluting stents might be associated with an adverse vessel response because of increased drug/polymer toxicity and lesion rigidity. METHODS AND RESULTS Lesions treated with overlapping everolimus- (EES=36) or paclitaxel-eluting stents (PES=38) were analyzed for 8-9-months by 3-dimensional intravascular ultrasound. EES were associated with significantly greater neointimal suppression in the single-strut regions than PES, with a similar trend in the overlap region. PES had significant vessel expansion in all regions, whereas there were no changes with EES. Neither stent fracture nor late incomplete stent apposition (LISA) in the overlap region was observed. CONCLUSIONS Overlapping EES appears to be effective without vessel expansion, stent fracture or LISA for up to 8-9 months.
An evaluation of the effect of gonadotropin-releasing hormone analogs and medroxyprogesterone acetate on uterine leiomyomata volume by magnetic resonance imaging: a prospective, randomized, double blind, placebo-controlled, crossover trial.

The purpose of this study was to prospectively compare the effectiveness of administering medroxyprogesterone acetate (MPA; 20 mg/day) in either the first (protocol A) or last (protocol B) 12-week period along with a 6-month course of the GnRH analog (GnRH-a; leuprolide acetate; 1 mg/day, sc) on uterine and leiomyomata volumes and hormone (estradiol, LH, and FSH) and serum lipid (total cholesterol, triglycerides, and high and low density lipoprotein) levels. Sixteen women were randomized into protocol A or B, received either MPA or placebo along with GnRH-a, respectively, and were then crossed over at 12 weeks to placebo or MPA, respectively, for the final 12-week interval of GnRH-a therapy. Total, myoma, and nonmyoma uterine volumes were determined by magnetic resonance imaging, and serum studies were performed at the beginning of the study and at 12 and 24 weeks. In both protocols, LH and estradiol levels declined by 80-90% (P < 0.03) and 55-72% (P < 0.02) of the baseline, respectively, at 12 weeks and remained at this level at 24 weeks. There were no significant changes in the other laboratory tests between protocols or longitudinally over time. Total uterine volume decreased to 73% of the baseline at 12 weeks in protocol B (P < 0.04), but did not change in protocol A. After crossover at 12 weeks, the total uterine volume of women in protocol A decreased to 74% of the baseline (P < 0.02) at 24 weeks. Between-protocol comparisons demonstrated a greater decline in total uterine volume in protocol B than A at 12 weeks, but after cross-over, MPA addition was associated with a significant increase in total uterine volume (protocol B) compared to a decrease in protocol A at 24 weeks (P < 0.005). In contrast, although myoma volume declined in both protocols, no significant changes in myoma volume were detected within or between groups over the treatment period. Nonmyoma volume changes in protocols A and B roughly paralleled total uterine volume changes, with MPA coadministration showing a correlation with a reversal in the GnRH-a-associated decrease in nonmyomatous tissue volume. (ABSTRACT TRUNCATED AT 400 WORDS)
The KBG syndrome: Confirmation of autosomal dominant inheritance and further delineation of the phenotype

We report on a Turkish family in which the father and his two sons were diagnosed as having the KBG syndrome. Large upper central incisors were the diagnostic finding in all three patients along with mental retardation, cryptorchidism, skeletal abnormalities, and short stature. Our report clearly confirms that the inheritance is autosomal dominant in KBG syndrome, although a high male to female ratio has been observed in published cases. © 2004 Wiley‐Liss, Inc.
Risk for new onset of depression during the menopausal transition: the Harvard study of moods and cycles.

CONTEXT Transition to menopause has long been considered a period of increased risk for depressive symptoms. However, it is unclear whether this period is one of increased risk for major depressive disorder, particularly for women who have not had a previous episode of depression. OBJECTIVE To examine the association between the menopausal transition and onset of first lifetime episode of depression among women with no history of mood disturbance. DESIGN Longitudinal, prospective cohort study. SETTING A population-based cross-sectional sample. PARTICIPANTS Premenopausal women, 36 to 45 years of age, with no lifetime diagnosis of major depression (N = 460), residing in 7 Boston, Mass, metropolitan area communities. Main Outcome Measure Incidence of new onset of depression based on structured clinical interviews, Center for Epidemiologic Studies Depression Scale scores, and an operational construct for depression. RESULTS Premenopausal women with no lifetime history of major depression who entered the perimenopause were twice as likely to develop significant depressive symptoms as women who remained premenopausal, after adjustment for age at study enrollment and history of negative life events. The increased risk for depression was somewhat greater in women with self-reported vasomotor symptoms. CONCLUSIONS The current study suggests that within a similarly aged population of women with no lifetime history of depression, those who enter the menopausal transition earlier have a significant risk for first onset of depression. Further studies are needed to determine more definitively whether other factors, such as the presence of vasomotor symptoms, use of hormone therapy, and the occurrence of adverse life events, independently modify this risk. Physical symptoms associated with the menopausal transition and mood changes seen during this period may affect many women as they age and may lead to a significant burden of illness.
T-cell responses to highly conserved CD4 and CD8 epitopes on the outer membrane protein of bovine leukemia virus: relevance to vaccine development

Bovine leukemia virus (BLV) is a retrovirus that infects cattle and sheep and may provide a model for studying human leukemia. Cell-mediated immune mechanisms may play a major role in protection against BLV infection. We describe here for the first time the identification of proliferative (CD4) and cytotoxic T-lymphocyte (CD8) epitopes of the gp51 envelope (env) protein of BLV. This protein and a recombinant form expressed by a vaccinia virus construct have been shown to be potential vaccine candidates. A complete series of overlapping peptides, 20 amino acids in length, was prepared to identify epitopes from gp51. These peptides were tested for the ability to elicit peripheral blood lymphocyte proliferation and cytotoxic T-lymphocyte responses in infected and uninfected cattle and sheep. Peptides 51-70 and 61-80 produced a proliferative response in lymphocytes from only uninfected animals (both sheep and cattle), and this was shown by T-cell subset deletion to be a CD4-mediated response. Seven BLV-infected sheep did not show a response to either peptide. Cytotoxic T-lymphocyte activity, however, was associated only with peptides 121-140 and 131-150. In this case, the response was demonstrated to be CD8 dependent and was found only in BLV-infected animals (sheep). Knowledge of the location of these T-cell recognition domains will complement data available on B-cell epitopes in gp51 and may be useful in the design of a subunit vaccine.
Withdrawal from repeated administration of morphine alters histamine-induced anxiogenic effects produced by intra-ventral hippocampal microinjection

In the present study, the influence of withdrawal from repeated administration of morphine on intra-ventral hippocampal microinjection of histamine-induced anxiety-like behavior was investigated in male Wistar rats. Three days subcutaneous administration of morphine (5—10 mg/kg) followed by five days free of the drug decreased the percentage open arm time and the percentage open arm entries. Intra-ventral hippocampal administration of histamine (2.5—7.5 µg/rat) decreased percentage open arm time and percentage open arm entries. Intra-ventral hippocampal histamine-induced anxiogenic effect was reversed in animals that had previously received the three days morphine (7.5 mg/kg) followed by five days free of the drug. Intra-ventral hippocampal administration of pyrilamine (5—20 µg/rat) or ranitidine (10—40 µg/rat) decreased percentage open arm time and percentage open arm entries. Pyrilamine- or ranitidine-induced anxiogenic effect was not changed in animals that had previously received the three days morphine (7.5 mg/kg) followed by five days free of the drug. Intra-ventral hippocampal injections of clobenpropit increased percentage open arm time. The percentage open arm time and percentage open arm entries were decreased in the morphine-treated animals compared with non-morphine-treated controls. Percentage open arm entries and locomotor activity was reduced with some doses of clobenpropit. It can be concluded that the histamine system is involved in anxiety-like behavior, and repeated injections of morphine followed by five days free of the drugs interact with histamine receptor mechanism.
The Pre-Iroquoian Pottery of New York State

The establishment of ceramic type categories for the pre-Iroquoian horizons of New York state, exclusive of the Tidewater area, was undertaken for a number of reasons. Primarily, it was believed that such an analysis, breaking down the existing ware divisions into finer type inventories, would prove useful in obtaining a more minute chronological differentiation of the Owasco and Point Peninsula cultures than would be possible on any other basis, and in so doing, would aid in clarifying their possible relationships as well as their conceivable affinities with other major ceramic manifestations in the New York area. It was also hoped that our survey might reveal significant areal subdivisions which eventually might be referred to historic tribal units. Finally, the achievement of a pottery typology for New York would elucidate problems of prehistoric culture diffusion and development over the Northeastern area and might, indeed, contribute to broader interpretations of cultural dynamics and processes of acculturation involved in the complex interconnections of sequential archaeological components.
Proceedings of the 12th International Workshop on High-Level Parallel Programming Models and Supportive Environments

The 12th HIPS workshop is a full-day meeting held at the IPDPS 2007 focusing on highlevel programming of chip multi-processors (multi-core PCs), computing clusters, and massively-parallel machines. Like its predecessors, the workshop seeks cross-fertilizing research in areas of parallel applications, language design, compilers, run-time systems, and programming tools. It provides a timely and lightweight forum for scientists and engineers to present the latest ideas, findings, and tools in these rapidly changing fields.
Diagnosis and prognosis of acute pulmonary embolism: focus on serum troponins

Pulmonary embolism is a common disease associated with high mortality. Death due to pulmonary embolism occurs mainly before hospital admission or in the first hours of the hospital stay. Prompt diagnosis and prognostic stratification and more intensive treatment in patients with estimated high risk for adverse outcomes have the potential to reduce mortality due to pulmonary embolism. Significant advances have recently been made in the risk stratification for adverse outcomes in patients with pulmonary embolism and normal blood pressure. In these patients, right ventricle overload assessed by echocardiography and probably by helical computerized tomography is a predictor of in-hospital mortality. Serum troponin is rapidly available in the emergency room, and has a critical role in the management of patients with acute coronary syndromes. The role of serum troponin in patients with pulmonary embolism has been explored recently: it seems to be marginal in diagnosis while it can significantly contribute to prognostic stratification. Elevated serum levels of troponins are associated with right ventricular overload and adverse in-hospital outcomes in patients with pulmonary embolism and normal blood pressure.
How to empower employees: using training to enhance work units’ collective empowerment

Purpose - – The purpose of this paper is to examine, theoretically and empirically, whether an employee training program can enhance the collective perception of empowerment of work units within an organization. The authors hypothesized that training participation relates to empowerment by enhancing the potency, meaningfulness, impact, and autonomy of the employees. Design/methodology/approach - – The authors collected data at two time points, before and after the training intervention. Over the two periods, the sample consisted of an average of 2,383 employees nested in 36 work units of a large multinational company. Findings - – The results indicated a positive relationship between training participation and increased levels of collective psychological empowerment, with differential effects on the dimensions of empowerment. Practical implications - – This study provides evidence of the positive relationship between training and empowerment, suggesting training effects across levels of analysis. The results indicated dimensions of empowerment that are more and such that are less prone to training. Such knowledge may help to inform organizations in developing training strategies. The authors provide recommendations for a respective training program. Originality/value - – This is one of the first studies to investigate the relationship between training participation of individual employees and shared empowerment perceptions within their work units, adding an important antecedent to the research on empowerment. In addition, the authors propose ways of how individual employees can affect shared perceptions among work-unit members. The study offers insights into the development of empowered work units, the vertical transfer of training across levels of analysis and implications for training programs.