Miracle-dz/UniFAR-FTUs huggingface dataset:
Consequences of Team Job Demands: Role Ambiguity Climate, Affective Engagement, and Extra-Role Performance

In the absence of clearly established procedures in the workplace, employees will experience a negative affective state. This situation influences their well-being and their intention to behave in ways that benefit the organization beyond their job demands. This impact is more relevant on teamwork where members share the perception of ambiguity through emotional contagion (role ambiguity climate). In the framework of the job demands-resources model, the present study analyzes how high levels of role ambiguity climate can have such an effect to reduce employee affective engagement. Over time it has been associated with negative results for the organization due to a lack of extra-role performance. The sample included 706 employees from a multinational company, who were divided into 11 work teams. In line with the formulated hypotheses, the results confirm the negative influence of the role ambiguity climate on extra-role performance, and the mediated effect of affective engagement in the relationship between the role ambiguity climate and extra-role performance. These findings indicate that the role ambiguity climate is related to the adequate or inadequate functioning of employees within a work context.
Inhibition of Human CYP3A4, UGT1A6, and P-glycoprotein with Halogenated Xanthene Food Dyes and Prevention by Superoxide Dismutase

Synthetic food dyes are xenobiotics, and, after ingestion, portions of these dyes may be absorbed and metabolized by phase I and II drug-metabolizing enzymes, and excreted by transporters of phase III enzymes. In the previous report, it was shown that inhibition of UDP-glucuronosyltrasnferase 1A6 occurred following ingestion of phloxine, erythrosine, and rose bengal present in 12 permitted synthetic food dyes. In this report, the influence of dyes was examined on CYP3A4, a major phase I drug-metabolizing enzyme, and P-glycoprotein, a major transporter by synthetic food dyes. Human cytochrome P-450 (CYP) 3A4 and P-glycoprotein were inhibited by xanthene food dyes. The IC50 values of these dyes to inhibit CYP3A4 and P-glycoprotein were the same as the level of inhibition of UGT1A6 produced by three haloganated xanthene food dyes in the previous report, except acid red, which inhibited only CYP3A4. Data suggest that inhibition by dyes is not enzyme specific but may be in a membrane-specific or protein-specific manner, such as conformational changes in protein. In the previous study, it was suggested that inhibition by dyes depended upon light irradiation due to generation of 1O2 from these dyes. In this study, the influence of superoxide dismutase and catalase on inhibition by dyes was examined. Superoxide dismutase but not catalase was effective in preventing the inhibition of UGT1A6 by the dyes. Data suggest that superoxide anions, originating from dyes via light irradiation, may attack drug-metabolizing enzymes. It is possible that red cosmetics containing phloxine, erythrosine, or rose bengal react with proteins in skin and may lead to skin damage.
The Motivation/Attitude-Driven Behavior (MADB) Model in E-Learning and the Effects on Mouse Dynamics

This paper presents the application of Motivation/Attitude-driven Behavior MADB model proposed by Wang Wang, 2007a in the e-learning context. The authors' work demonstrates how mathematical models and formal cognitive processes is developed based on menu search task. The effects of menu design on stress perceptions, motivation and attitudes during the search tasks are being examined. The correlations between emotion stress perception, motivation, attitude, decision, behavior and mouse behavior are studied. The authors' findings are consistent with what was proposed by Wang. They also found that behavior is significantly correlated to mouse dynamics, such as mouse speed, mouse idle duration and mouse left click rate. Generally strong behavior strength results in slower mouse movements. The significant effect of behavior on mouse dynamics may be caused by motivation and decision, but not attitude and stress perception.
Changes in Trypanosoma cruzi Kinetoplast DNA Minicircles Induced by Environmental Conditions and Subcloning

ABSTRACT. Reversible changes in kinetoplast DNA (kDNA) minicircles sequences were observed in clones of Trypanosoma cruzi strain Y, following a number of passages during exponential growth phase or after subcloning in blood‐free medium. kDNA restriction patterns of clones were similar to those of the original uncloned strain, while subclones presented distinct kDNA restriction patterns. Homology experiments demonstrated strong hybridization between kDNA with the same electrophoretic mobility patterns while only weak signals were observed with kDNA of different patterns. The changes observed, which are unprecedented in T. cruzi clones, characterize transkinetoplastidy, and seem to be associated with similarly reversible changes both in zymodeme and in infectivity.
Structural constraints and importance of caffeic acid moiety for anti-hyperglycemic effects of caffeoylquinic acids from chicory.

SCOPE Chicory (Cichorium intybus L.) is a perennial herb often consumed as a vegetable, whereas the ground and roasted roots are blended as a coffee substitute. Caffeoylquinic or chlorogenic acids (CQA), the abundant intermediates of lignin biosynthesis in chicory, have been reported to improve glucose metabolism in humans, but the functional group in their structure responsible for this effect has not been yet characterized. METHODS AND RESULTS Here, we showed that three di-O-caffeoylquinic acids suppressed hepatic glucose production in H4IIE rat hepatoma cells by reducing expression of glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), two key enzymes that regulate hepatic gluconeogenesis. Direct comparisons between CQAs and their metabolites (3-caffeoylquinic, caffeic, and quinic acids) revealed the caffeic acid moiety alone was responsible for the observed effects. Further analysis suggested the activation of PI3K and MAPK pathways as a method of controlling gene expression was shared between caffeoylquinic and caffeic acids. These compounds promoted increased mitochondrial respiration and cellular metabolism, in part by inducing oxidative phosphorylation and proton leak. CONCLUSION We concluded that the caffeic acid moiety was important for suppression of hepatic gluconeogenesis and hyperglycemia, ultimately strengthening the link between dietary interventions based on caffeic acid-containing plant foods and healthy glucose metabolism.
A CIRCULAR-CIRCULAR REGRESSION MODEL

This paper provides a regression model in which both covariates and re- sponses are angular variables. The regression curve is expressed as a form of the Mobius circle transformation. The angular error is assumed to follow a wrapped Cauchy or, equivalently, circular Cauchy distribution. A bivariate circular distribu- tion is proposed to model our circular regression. Some properties of the regression, including estimation and testing procedures, are obtained. The proposed methods are applied to marine biology and wind direction data.
[Assessment of radiation dose to patients in hysterosalpingography].

INTRODUCTION The optimization principle applied to the patient radioprotection, recently introduced in Italian legislation, requires a careful analysis of the working procedures and clinical protocols generally used in radiological practice, to avoid unnecessary exposures with no loss in diagnostic information. We carried out this analysis in hysterosalpingography, which is the radiological examination usually performed on fertile women to detect uterine and tubal conditions. MATERIALS AND METHODS The dosimetric survey was carried out on 35 patients, 22 to 40 years old, to evaluate: a) entrance dose and dose x area product; b) doses to the most irradiated organs, that is ovaries and uterus; c) effective dose equivalent and effective dose. The doses were measured with LiF thermoluminescent dosimeters, while an ionization chamber enabled us to the calculate dose area product. Ovaries and uterus doses were calculated with a Monte Carlo program using skin entrance exposure data and some technical parameters of the examination. RESULTS The good agreement of the results obtained with both dosimetric methods demonstrated their equivalence in this specific case where the use of a clinical protocol, with no lateral projection, made the dosimeters lie always within the X-ray beam. DISCUSSION AND CONCLUSIONS The mean dose was 4.5 mGy for the ovaries and 6.2 mGy for the uterus. Following the recommendations of the International Commission on Radiological Protection, both effective dose equivalent, with a mean value of 2.0 mSv, and effective dose, with a mean value of 1.95 mSv, were estimated. In conclusion, our results show that the patient dose can be reduced by limiting the use of fluoroscopy, using small size films and decreasing the number of exposures.
Man to Man and Side by Side, They Cope with Prostate Cancer

Abstract Prostate cancer affects men and their loved ones; consequently, survivors and their wives can gain from social support throughout the illness experience. After observing meetings of a support group for prostate cancer survivors and their partners, the authors used the constant comparison method to draw conclusions about the types of support generated in the men's and women's divisions of the group. The authors concluded that both divisions served as sites of information but not as scenes of practical assistance. The authors also found that the discursive practices of the groups and the structural elements of the group meetings inhibited emotional support through topic turning, comparisons between members, and the role of group facilitators. The authors consider the study's implications for support group leaders and scholars.
Clinical and experimental studies in nickel allergy.

Kliniska och experimentella studier av nickelallergi Nickelallergi ar vanligt, sarskilt hos kvinnor. Nickelallergi leder ofta till periodiskt eller kroniskt eksem, framst pa handerna. Dessa eksem kan vara svarlakta och leda till langa sjukskrivningsperioder. Nickel finns overallt i omgivningen och det gar inte att helt undvika exposition for denna metall. Clinical and experimental studies in nickel allergy Contact allergy to nickel is very common among females and frequently leads to recurrent or chronic hand eczema, which often is hard to treat and leads to long sick-leave periods. Nickel is ubiquitous and total avoidance of exposure to the allergen is impossible. The aim of the present study was to get better understanding of various factors of possible significance for allergic contact dermatitis from nickel. A large inter- and intraindividual variation in nickel patch test reactivity was found in nickel-hypersensitive persons. Thus, no one had the same reactivity at repeated testing, and also completely negative tests were seen. A previous dermatitis was shown to be important for the elicitation of allergic contact dermatitis from nickel on topical nickel exposure. A previous nickel dermatitis gave an increased reactivity while a previous, irritant (SLS) contact dermatitis gave a decreased reactivity. The time interval from the previous allergic or irritant contact dermatitis was important for reactivity. Flare-up reactions after oral challenge with nickel were found to be correlated to nickel dose, intensity of a previous eczema, and time after the eczema. Orally administered nickel results in increased nickel in urine and less increase of nickel in faeces in atopics compared to controls. This may indicate increased nickel absorption in atopics. There was also a positive correlation between nickel in urine and TIBC in atopics which may indicate an interesting therapeutic possibility to interfere with the nickel absorption.
Role of pericellular matrix in development of a mechanically functional neocartilage.

The role of the chondrocyte pericellular matrix (PCM) was examined in a three-dimensional chondrocyte culture system to determine whether retention of the native pericellular matrix could stimulate collagen and proteoglycan accumulation and also promote the formation of a mechanically functional hyaline-like neocartilage. Porcine chondrocytes and chondrons, consisting of the chondrocyte with its intact pericellular matrix, were maintained in pellet culture for up to 12 weeks. Sulfated glycosaminoclycans and type II collagen were measured biochemically. Immunocytochemistry was used to examine collagen localization as well as cell distribution within the pellets. In addition, the equilibrium compressive moduli of developing pellets were measured to determine whether matrix deposition contributed to the mechanical stiffness of the cartilage constructs. Pellets increased in size and weight over a 6-week period without apparent cell proliferation. Although chondrocytes quickly rebuilt a PCM rich in type VI collagen, chondron pellets accumulated significantly more proteoglycan and type II collagen than did chondrocyte pellets, indicating a greater positive effect of the native PCM. After 5 weeks in chondron pellets, matrix remodeling was evident by microscopy. Cells that had been uniformly distributed throughout the pellets began to cluster between large areas of interterritorial matrix rich in type II collagen. After 12 weeks, clusters were stacked in columns. A rapid increase in compressive strength was observed between 1 and 3 weeks in culture for both chondron and chondrocyte pellets and, by 6 weeks, both had achieved 25% of the equilibrium compressive stiffness of cartilage explants. Retention of the in vivo PCM during chondrocyte isolation promotes the formation of a mechanically functional neocartilage construct, suitable for modeling the responses of articular cartilage to chemical stimuli or mechanical compression.
Serological comparison of bovine T-mycoplasmas.

Summary: Eight bovine T-mycoplasmas examined serologically by the metabolism inhibition test, growth inhibition on agar and by immunofluorescence showed considerable serological heterogeneity. None of the eight strains was serologically identical with any other, but three formed a group of similar organisms. No evidence was obtained for one particular serotype being confined to a specific anatomical site or being isolated typically from any particular pathological condition. Antisera to the bovine T-mycoplasmas were tested against eight serologically distinct human strains and a caprine, a simian and a canine T-mycoplasma. None of the non-bovine strains was identical to these bovine T-mycoplasmas.
Role of Type I IFNs in Pulmonary Complications of Pneumocystis murina Infection 1

Despite the advent of highly active antiretroviral therapy, pulmonary complications in AIDS are a common clinical problem. Pneumocystis jiroveci infection causes a life-threatening pneumonia, especially in individuals with CD4 T cell deficiencies as occurs in AIDS. Although Pneumocystis sp. is an extracellular fungal pathogen, CD8 T cells are the predominant lymphocyte recruited to the lung in CD4-deficient humans and mice during Pneumocystis pneumonia, and we have found that these CD8 T cells are responsible for subsequent lung damage in CD4 T cell-depleted mice. Comparing CD4 T cell-depleted IFN-α receptor knockout (KO) mice to wild-type mice, we found that this CD8 T cell recruitment and lung damage is type I IFN (IFN-αβ) dependent. However, in both CD4 competent, wild-type and IFN-α receptor (IFNAR) KO mice, Pneumocystis infection leads to an eosinophilic granulocyte influx with bronchial epithelial changes as seen in asthma. This response is delayed in IFNAR KO mice, as is pathogen clearance. Although the inflammation is transient in wild-type animals and resolves upon Pneumocystis clearance, it is more severe and persists through day 35 postinfection in IFNAR KO mice, leading to fibrosis. In addition, IFNAR KO, but not wild-type, mice mount a Pneumocystis-specific IgE response, an indicator of allergic sensitization. Thus, in the absence of IFNAR signaling and CD4 T cells, Pneumocystis-mediated lung damage does not occur, whereas in CD4-competent animals, the absence of IFNAR signaling results in an exacerbated Th2 response, asthma-like symptoms, and fibrosis. Therefore, both CD4 T cell- and type I IFN-mediated mechanisms can determine pulmonary complications from Pneumocystis infection.
Inactivation of mecA prevents recovery from the competent state and interferes with cell division and the partitioning of nucleoids in Bacillus subtilis

The development of genetic competence in Bacillus subtilis requires the synthesis of ComK, a transcription factor, which is normally produced as a culture enters the stationary phase. This synthesis is known to be regulated in part by the protein MecA. Loss‐of‐function mutations in mecA result in overexpression of ComK and its appearance early during exponential growth. We show here that mecA inactivation also causes a loss of colony‐forming ability, especially during stationary phase. This loss is accompanied by the appearance of cells in which normal nucleoid separation has failed to occur. Renografin gradient fractionation of mecA cultures grown to competence reveals that nearly 100% of the cells band at the low buoyant density characteristic of competent cells, and that this low density is competence‐related. The loss of viability, the low buoyant density and the nucleoid separation defect, are all comK‐dependent. The loss of viability can be reversed by even the transient introduction of mecA+. It is proposed that these effects of ComK overexpression are related to the DNA replication arrest normally exhibited by the competent cell fraction and that MecA is needed to reverse this arrest and to permit escape from the competent state. The shift of nearly 100% of the cells to light buoyant density in a mecA mutant culture strongly suggests that the MecA protein is a regulator of the cell‐type‐specific expression of competence.
Noninvasive ventilation during exercise training improves exercise tolerance in patients with chronic obstructive pulmonary disease.

PURPOSE In patients with chronic obstructive pulmonary disease, pulmonary rehabilitation has been demonstrated to increase exercise capacity and reduce dyspnea. In the most disabled patients, the intensity of exercise during the training sessions is limited by ventilatory pump capacity. This study therefore evaluated the beneficial effect of noninvasive ventilation (NIV) support during the rehabilitation sessions on exercise tolerance. METHODS This study included 14 patients with stabilized chronic obstructive pulmonary disease, ages 63 +/- 7 years, with a forced expiratory volume in 1 second (FEV(1)) 31.5% +/- 9.2% of predicted value. All 14 patients participated in an outpatient pulmonary rehabilitation program. Seven of the patients trained with NIV during the exercise sessions (NIV group), whereas the remaining seven patients breathed spontaneously (control group). Exercise tolerance was evaluated during an incremental exercise test and during constant work rate exercise at 75% of peak oxygen consumption (VO(2)) before and after the training program. RESULTS The application of noninvasive ventilation increased exercise tolerance, reduced dyspnea, and prevented exercise-induced oxygen desaturation both before and after training. The pressure support was well tolerated by all the patients during the course of the training program. In the NIV group, training induced a greater improvement in peak VO(2) (18% vs 2%; P <.05) and a reduced ventilatory requirement for maximal exercise, as compared with the control group. The constant work rate exercise duration increased similarly in both groups (116% vs 81%, nonsignificant difference), and posttraining blood lactate was decreased at isotime (P <.05 in both groups), but not at the end of the exercise. CONCLUSION In this pilot study, exercise training with noninvasive ventilation support was well tolerated and yielded further improvement in the increased exercise tolerance brought about by pulmonary rehabilitation in patients with chronic obstructive pulmonary disease. This improved exercise tolerance is partly explained by a better ventilatory adaptation during exercise.
Postsecondary Education and Dementia Risk in Older Jesuit Priests

Lack of early childhood education has been implicated as a risk factor for dementia in late life. It is unclear whether dementia risk is also associated with less education in the adult years. The purpose of the present study was to determine whether amount of postsecondary education (i.e., education beyond the high school or 12th-grade level) is associated with dementia after age 60. Cognitive function (assessed by a neuropsychological test battery) and the prevalence of dementia (assessed by clinical criteria) were determined in 86 Jesuit priests (age 60 to 98) who had from 0 to 23 years of postsecondary education. Logistic regression was used to estimate the odds of dementia as a function of postsecondary education. The risk of dementia was increased in those with less postsecondary education (odds ratio = 3.4; 95% confidence interval, 1.2 to 10.0) after adjustment for age, IQ, and depression. These findings support an association between amount of postsecondary education and risk of dementia in late life.
New approaches for cooperative use of multiple antennas in ad hoc wireless networks

The paper explores the interaction between cooperative diversity techniques, in the physical layer, and routing, in the network layer. Three approaches are proposed: relay-by-flooding; relay-assisted routing; relay-enhanced routing. In relay-by-flooding, the tradeoff between achieved rate and required power is studied for three selective decode-and-forward schemes: simple relay; space-time-coded relay; best-select relay. In relay-enhanced routing, cooperation is applied to each link of an existing route. Two relay selection approaches are proposed: best-select in the neighbor set; best-select in the decoded set. Best-select in the decoded set is shown to improve the performance significantly.