Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Low versus High Intravenous Amino Acid Intake on Very Low Birth Weight Infants in the Early Neonatal Period

Greater protein intakes are required than have been commonly used to achieve fetal in utero protein accretion rates in preterm neonates. To study the efficacy and safety of more aggressive amino acid intake, we performed a prospective randomized study in 28 infants [mean wt, 946 ± 40 g (SEM)] of 1 (low amino acid intake, LAA) versus 3 g·kg−1·d−1 (high amino acid intake, HAA) at 52.0 ± 3.0 h of life. After a minimum of 12 h of parenteral nutrition, efficacy was determined by protein balance and was significantly lower in the LAA versus HAA groups by both nitrogen balance (−0.26 ± 0.11 versus 1.16 ± 0.15 g·kg−1·d−1, p < 0.00005) and leucine stable isotope (0.184 ± 0.17 versus 1.63 ± 0.20 g·kg−1·d−1, p < 0.0005) methods. Leucine flux and oxidation and nonoxidative leucine disposal rates were all significantly higher in the HAA versus LAA groups (249 ± 13 versus 164 ± 8, 69 ± 5 versus 32 ± 3, and 180 ± 10 versus 132 ± 8 μmol·kg−1·h−1, respectively, p < 0.005), but leucine appearance from protein breakdown was not (140 ± 15 in HAA versus 128 ± 8 μmol·kg−1·h−1). In terms of possible toxicity with HAA, there were no significant differences between groups in the amount of sodium bicarbonate administered, degree of acidosis as determined by base deficit, or blood urea nitrogen concentration. Parenteral HAA versus LAA intake resulted in increased protein accretion, primarily by increasing protein synthesis versus suppressing protein breakdown, and appeared to be well tolerated by very preterm infants in the first days of life.
Action and Outcome Encoding in the Primate Caudate Nucleus

The basal ganglia appear to have a central role in reinforcement learning. Previous experiments, focusing on activity preceding movement execution, support the idea that dorsal striatal neurons bias action selection according to the expected values of actions. However, many phasically active striatal neurons respond at a time too late to initiate or select movements. Given the data suggesting a role for the basal ganglia in reinforcement learning, postmovement activity may therefore reflect evaluative processing important for learning the values of actions. To better understand these postmovement neurons, we determined whether individual striatal neurons encode information about saccade direction, whether a reward had been received, or both. We recorded from phasically active neurons in the caudate nucleus while monkeys performed a probabilistically rewarded delayed saccade task. Many neurons exhibited peak responses after saccade execution (77 of 149) that were often tuned for the direction of the preceding saccade (61 of 77). Of those neurons responding during the reward epoch, one subset showed direction tuning for the immediately preceding saccade (43 of 60), whereas another subset responded differentially on rewarded versus unrewarded trials (35 of 60). We found that there was relatively little overlap of these properties in individual neurons. The encoding of action and outcome was performed by largely separate populations of caudate neurons that were active after movement execution. Thus, striatal neurons active primarily after a movement appear to be segregated into two distinct groups that provide complimentary information about the outcomes of actions.
Integrated stereological and biochemical studies on hepatocytic membranes. II. Correction of section thickness effect on volume and surface density estimates

The basic stereological formulas for estimating volume (Vv) and surface (Sv) densities are strictly valid only for true infinitely thin sections; the use of "ultrathin" sections of finite thickness T introduces systematic errors, mostly in the sense of overestimation of the parameters. These errors depend on the size and shape of the structural elements and on T. Correction factors for this effect of T are derived by considering model structures that simulate the shape and arrangement of subcellular organelles: (a) spherical vesicles, (b) disks as models for rough endoplasmic reticulum (RER) cisternae, (c) cylindrical tublules as models for smooth endoplasmic reticulum (SER) tubules, microvilli, etc. For vesicles, a model of discrete convex spherical particles is assumed; the correction factors consider loss of caps due to grazing sections and size distribution of the vesicles. The disk and tubule models are used in connection with the new integral geometric formulas of R.E. Miles which consider random aggregates of "inter-penetrating" particles so that the resultant structure is non- convex and thus approximates in nature the networks characteristic of endoplasmic reticulum (ER). Some practical examples relative to liver cells show that the errors due to section thickness may be of the order of 20-40% or more. Computation formulas as well as graphs are given for the determination of the correction factors for Vv and Sv.
Male Circumcision and HIV Control in Africa

Jennifer Vines This is the fi rst of 12 Correspondence pieces in this issue that are in response to an important PLoS Medicine Research Article: Auvert B, Taljaard D, Lagarde E, Sobngwi-Tambekou J, Sitta R, et al. (2005) Randomized, controlled intervention trial of male circumcision for reduction of HIV infection risk: The ANRS 1265 trial. PLoS Med 2(11): e298. Readers wishing to add their own views may do so using our E-letters facility, where the debate continues.
Pseudoalteromonas arabiensis sp . nov . , a marine polysaccharide-producing bacterium

Correspondence Hidetoshi Matsuyama matsuyama@tspirit.tokai-u.jp School of Biological Science and Engineering, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan School of Marine Science and Technology, Tokai University, Orido 3-20-1, Shimizu-ku, Shizuoka 424-8610, Japan Center for advanced Marine Core Research, Kochi University, B200 Nankoku, Kochi 783-0032, Japan Bioprocess Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
Æminium: A Permission-Based Concurrent-by-Default Programming Language Approach

Writing concurrent applications is extremely challenging, not only in terms of producing bug-free and maintainable software, but also for enabling developer productivity. In this article we present the Æminium concurrent-by-default programming language. Using Æminium programmers express data dependencies rather than control flow between instructions. Dependencies are expressed using permissions, which are used by the type system to automatically parallelize the application. The Æminium approach provides a modular and composable mechanism for writing concurrent applications, preventing data races in a provable way. This allows programmers to shift their attention from low-level, error-prone reasoning about thread interleaving and synchronization to focus on the core functionality of their applications. We study the semantics of Æminium through μÆminium, a sound core calculus that leverages permission flow to enable concurrent-by-default execution. After discussing our prototype implementation we present several case studies of our system. Our case studies show up to 6.5X speedup on an eight-core machine when leveraging data group permissions to manage access to shared state, and more than 70% higher throughput in a Web server application.
The Oncogene PDRG1 Is an Interaction Target of Methionine Adenosyltransferases

Methionine adenosyltransferases MAT I and MAT III (encoded by Mat1a) catalyze S-adenosylmethionine synthesis in normal liver. Major hepatic diseases concur with reduced levels of this essential methyl donor, which are primarily due to an expression switch from Mat1a towards Mat2a. Additional changes in the association state and even in subcellular localization of these isoenzymes are also detected. All these alterations result in a reduced content of the moderate (MAT I) and high Vmax (MAT III) isoenzymes, whereas the low Vmax (MAT II) isoenzyme increases and nuclear accumulation of MAT I is observed. These changes derive in a reduced availability of cytoplasmic S-adenosylmethionine, together with an effort to meet its needs in the nucleus of damaged cells, rendering enhanced levels of certain epigenetic modifications. In this context, the putative role of protein-protein interactions in the control of S-adenosylmethionine synthesis has been scarcely studied. Using yeast two hybrid and a rat liver library we identified PDRG1 as an interaction target for MATα1 (catalytic subunit of MAT I and MAT III), further confirmation being obtained by immunoprecipitation and pull-down assays. Nuclear MATα interacts physically and functionally with the PDRG1 oncogene, resulting in reduced DNA methylation levels. Increased Pdrg1 expression is detected in acute liver injury and hepatoma cells, together with decreased Mat1a expression and nuclear accumulation of MATα1. Silencing of Pdrg1 expression in hepatoma cells alters their steady-state expression profile on microarrays, downregulating genes associated with tumor progression according to GO pathway analysis. Altogether, the results unveil the role of PDRG1 in the control of the nuclear methylation status through methionine adenosyltransferase binding and its putative collaboration in the progression of hepatic diseases.
Predictability of load/store instruction latencies

Presents a model of coarse grain dataflow execution. The authors present one top down and two bottom up methods for generation of multithreaded code, and evaluate their effectiveness. The bottom up techniques start from a fine-grain dataflow graph and coalesce this into coarse-grain clusters. The top down technique generates clusters directly from the intermediate data dependence graph used for compiler optimizations. The authors discuss the relevant phases in the compilation process. They compare the effectiveness of the strategies by measuring the total number of clusters executed, the total number of instructions executed, cluster size, and number of matches per cluster. It turns out that the top down method generates more efficient code, and larger clusters. However the number of matches per cluster is larger for the top down method, which could incur higher cluster synchronization costs. <<ETX>>
Exposure to a Highly Caloric Palatable Diet during the Perinatal Period Affects the Expression of the Endogenous Cannabinoid System in the Brain, Liver and Adipose Tissue of Adult Rat Offspring

Recent studies have linked gestational exposure to highly caloric diets with a disrupted endogenous cannabinoid system (ECS). In the present study, we have extended these studies by analyzing the impact of the exposure to a palatable diet during gestation and lactation on a) the adult expression of endocannabinoid-related behaviors, b) the metabolic profile of adult offspring and c) the mRNA expression of the signaling machinery of the ECS in the hypothalamus, the liver and the adipose tissue of adult offspring of both sexes. Exposure to a palatable diet resulted in a) sex-dimorphic and perinatal diet specific feeding behaviors, including the differential response to the inhibitory effects of the cannabinoid receptor inverse agonist AM251, b) features of metabolic syndrome including increased adiposity, hyperleptinemia, hypertriglyceridemia and hypercholesterolemia and c) tissue and sex-specific changes in the expression of both CB1 and CB2 receptors and in that of the endocannabinoid-degrading enzymes FAAH and MAGL, being the adipose tissue the most affected organ analyzed. Since the effects were observed in adult animals that were weaned while consuming a normal diet, the present results indicate that the ECS is one of the targets of maternal programming of the offspring energy expenditure. These results clearly indicate that the maternal diet has long-term effects on the development of pups through multiple alterations of signaling homeostatic pathways that include the ECS. The potential relevance of these alterations for the current obesity epidemic is discussed.
Cochlear Implants Versus Hearing Aids in a Middle-Income Country: Costs, Productivity, and Quality of Life.

OBJECTIVE In Colombia there are three main treatment approaches for bilateral profound sensorineural hearing loss, also known as profound deafness (PD): cochlear implants (CI), hearing aids (HA), and no treatment (NT). The objective of this study is to determine the optimal treatment approach for PD in terms of productivity and cost-effectiveness. STUDY DESIGN Hearing levels and SES data were obtained from audiometric tests conducted on 100 patients with CI. For each treatment approach, productivity and cost-effectiveness assessments were estimated using influence diagrams and Monte Carlo simulations. Productivity was calculated as the net present value of a patient's lifetime income divided by total health-related and educational costs. For the CI and HA, the cost-effectiveness indicator was calculated as quality-adjusted life-years (QALYs) gained in comparison with the NT approach divided by the total cost of the treatment option. RESULTS The results for the CI, the HA, and NT in terms of productivity ratio were 1.53, 0.94, and 1.47, respectively. Patients using CI had a gain of 5.7 QALYs, whereas patients using HA had a gain of 4.6 QALYs. The results for the CI and the HA in terms of cost-effectiveness were $15,169 and $15,430 per QALY, respectively. CONCLUSION The CI was found to be the optimal treatment for PD, as it was the most efficient and cost-effective in terms of improving patients' productivity and quality of life. We observed that children who had received CI developed hearing and speech abilities that contributed to their productivity and quality of life to a greater extent than those with HA.
The Hippocampus and Imagining the Future: Where Do We Stand?

24 25 Recent neuroimaging work has demonstrated that the hippocampus is engaged when 26 imagining the future, in some cases more than when remembering the past. It is possible that 27 this hippocampal activation reflects recombining details into coherent scenarios and/or the 28 encoding of these scenarios into memory for later use. However, inconsistent findings have 29 emerged from recent studies of future simulation in patients with memory loss and 30 hippocampal damage. Thus, it remains an open question as to whether the hippocampus is 31 necessary for future simulation. In this review, we consider the findings from patient studies 32 and the neuroimaging literature with respect to a new framework that highlights three 33 component processes of simulation: accessing episodic details, recombining details, and 34 encoding simulations. We attempt to reconcile these discrepancies between neuroimaging 35 and patient studies by suggesting that different component processes of future simulation may 36 be differentially affected by hippocampal damage. 37 Hippocampus and imagining the future 3
Acute stress affects risk taking but not ambiguity aversion

Economic decisions are often made in stressful situations (e.g., at the trading floor), but the effects of stress on economic decision making have not been systematically investigated so far. The present study examines how acute stress influences economic decision making under uncertainty (risk and ambiguity) using financially incentivized lotteries. We varied the domain of decision making as well as the expected value of the risky prospect. Importantly, no feedback was provided to investigate risk taking and ambiguity aversion independent from learning processes. In a sample of 75 healthy young participants, 55 of whom underwent a stress induction protocol (Trier Social Stress Test for Groups), we observed more risk seeking for gains. This effect was restricted to a subgroup of participants that showed a robust cortisol response to acute stress (n = 26). Gambling under ambiguity, in contrast to gambling under risk, was not influenced by the cortisol response to stress. These results show that acute psychosocial stress affects economic decision making under risk, independent of learning processes. Our results further point to the importance of cortisol as a mediator of this effect.
A Comprehensive Spectroscopic and Computational Investigation to Probe the Interaction of Antineoplastic Drug Nordihydroguaiaretic Acid with Serum Albumins

Exogenous drugs that are used as antidote against chemotheray, inflammation or viral infection, gets absorbed and interacts reversibly to the major serum transport protein i.e. albumins, upon entering the circulatory system. To have a structural guideline in the rational drug designing and in the synthesis of drugs with greater efficacy, the binding mechanism of an antineoplastic and anti-inflammatory drug Nordihydroguaiaretic acid (NDGA) with human and bovine serum albumins (HSA & BSA) were examined by spectroscopic and computational methods. NDGA binds to site II of HSA with binding constant (Kb) ~105 M-1 and free energy (ΔG) ~ -7.5 kcal.mol-1. It also binds at site II of BSA but with lesser binding affinity (Kb) ~105 M-1 and ΔG ~ -6.5 kcal.mol-1. The negative value of ΔG, ΔH and ΔS for both the albumins at three different temperatures confirmed that the complex formation process between albumins and NDGA is spontaneous and exothermic. Furthermore, hydrogen bonds and hydrophobic interactions are the main forces involved in complex formation of NDGA with both the albumins as evaluated from fluorescence and molecular docking results. Binding of NDGA to both the albumins alter the conformation and causes minor change in the secondary structure of proteins as indicated by the CD spectra.
Effect of Chronic Athletic Activity on Brown Fat in Young Women

Background The effect of chronic exercise activity on brown adipose tissue (BAT) is not clear, with some studies showing positive and others showing negative associations. Chronic exercise is associated with increased resting energy expenditure (REE) secondary to increased lean mass and a probable increase in BAT. Many athletes are in a state of relative energy deficit suggested by lower fat mass and hypothalamic amenorrhea. States of severe energy deficit such as anorexia nervosa are associated with reduced BAT. There are no data regarding the impact of chronic exercise activity on BAT volume or activity in young women and it is unclear whether relative energy deficiency modifies the effects of exercise on BAT. Purpose We assessed cold induced BAT volume and activity in young female athletes compared with non-athletes, and further evaluated associations of BAT with measures of REE, body composition and menstrual status. Methods The protocol was approved by our Institutional Review Board. Written informed consent was obtained from all participants prior to study initiation. This was a cross-sectional study of 24 women (16 athletes and8 non-athletes) between 18–25 years of age. Athletes were either oligo-amenorrheic (n = 8) or eumenorrheic (n = 8). We used PET/CT scans to determine cold induced BAT activity, VMAX Encore 29 metabolic cart to obtain measures of REE, and DXA for body composition. Results Athletes and non-athletes did not differ for age or BMI. Compared with non-athletes, athletes had lower percent body fat (p = 0.002), higher percent lean mass (p = 0.01) and trended higher in REE (p = 0.09). BAT volume and activity in athletes trended lower than in non-athletes (p = 0.06; p = 0.07, respectively). We found negative associations of BAT activity with duration of amenorrhea (r = -0.46, p = 0.02). BAT volume correlated inversely with lean mass (r = -0.46, p = 0.02), and positively with percent body fat, irisin and thyroid hormones. Conclusions Our study shows a trend for lower BAT in young female athletes compared with non-athletes, and shows associations of brown fat with menstrual status and body composition. Brown fat may undergo adaptive reductions with increasing energy deficit.
History of Mathematics in the Turkish Middle School Mathematics Curriculum and Textbooks

The purpose of this study was to analyze how the history of mathematics incorporated into Turkish middle school mathematics curriculum (5 th -8 th grades) and textbooks. The data were collected through document analysis from the Turkish middle school mathematics curriculum guide (5 th -8 th grades) and six Turkish middle school mathematics textbook series approved by the National Board of Education. According to the results gathered from the math curriculum, only one reference of the history of mathematics was found in the sixth-grade learning objectives and a few suggestions acknowledging the use of the history of mathematics in the learning and teaching process. No trace of the history of mathematics found in the content and assessment aspects of the curriculum. Further, the total number of the instances of the history of mathematics found in the six Turkish middle school mathematics textbooks for 5 th -8 th grade is only twenty-seven. Most of the references found in the math textbooks were placed in the introduction part of the topic. Based on the findings, it is obvious that the history of mathematics was used like a rarely-found appetizer both in the curriculum and the textbooks.
Neutrophils and the calcium-binding protein MRP-14 mediate carrageenan-induced antinociception in mice.

BACKGROUND: We have previously shown that the calcium-binding protein MRP-14 secreted by neutrophils mediates the antinociceptive response in an acute inflammatory model induced by the intraperitoneal injection of glycogen in mice. AIM: In an attempt to broaden the concept that neutrophils and MRP-14 controls inflammatory pain induced by different type of irritants, in the present study, after demonstrating that carrageenan (Cg) also induces atinociception in mice, we investigated the participation of both neutrophils and MRP-14 in the phenomenon. METHODS: Male Swiss mice were injected intraperitoneally with Cg and after different time intervals, the pattern of cell migration of the peritoneal exudate and the nociceptive response of animals submitted to the writhing test were evaluated. The participation of neutrophils and of the MRP-14 on the Cg effect was evaluated by systemic inoculation of monoclonal antibodies anti-granulocyte and anti-MRP-14. RESULTS: Our results demonstrate that the acute neutrophilic peritonitis evoked by Cg induced antinociception 2, 4 and 8 h after inoculation of the irritant. Monoclonal antibodies anti-granulocyte or anti-MRP-14 reverts the antinociceptive response only 2 and 8 h after Cg injection. The antibody anti-MRP-14 partially reverts the antinociception observed after 4 h of Cg injection while the anti-granulocyte antibody enhances this effect. This effect is reverted by simultaneous treatment of the animals with both antibodies. After 4 h of Cg injection in neutrophil-depleted mice a significant expression of the calcium-binding protein MRP-14 was detected in the cytoplasm of peritoneal macrophages. This suggests that the enhancement of the effect observed after treatment with the anti-neutrophil antibody may be due to secretion of MRP-14 by macrophages. It has also been demonstrated that endogenous opioids and glucocorticoids are not involved in the antinociception observed at the 4th hour after Cg injection. CONCLUSION: These data support the hypothesis that neutrophils and the calcium-binding protein MRP-14 are participants of the endogenous control of inflammatory pain in mice despite the model of acute inflammation used.