Miracle-dz/UniFAR-FTUs huggingface dataset:
A B23-interacting sequence as a tool to visualize protein interactions in a cellular context

We report the characterization of a nucleolar localization sequence (NoLS) that targets the green fluorescent protein (GFP) into the granular component (GC) of nucleoli. This NoLS interacts in vitro specifically and directly with the major nucleolar protein B23 and more precisely with the region of B23 including the two acidic stretches. The affinity of NoLS for B23 is stronger than that of the HIV-1 Rev protein in vitro. Moreover, B23-NoLS interaction also occurs in vivo. Indeed, (1) NoLS confers on the GFP the behavior of B23 throughout the cell cycle, (2) the GFP-NoLS fusion and B23 remain colocalized after drug treatments, (3) a selective delocalization of B23 from nucleoli to nucleoplasm induces a concomitent delocalization of the GFP-NoLS fusion, and (4) the fusion of NoLS to fibrillarin makes it possible to colocalize fibrillarin and B23. Interestingly, by fusing NoLS to fibrillarin, both fibrillarin and the fibrillarin partner Nop56 are mislocalized in the GC of nucleoli. Similarly, by fusing the NoLS to MafG, part of the nuclear transcription factor NF-E2 composed of both MafG and p45 NF-E2, NF-E2 is redirected from the nucleoplasm to the nucleoli. Thus, we propose that the NoLS may be used as a tool to visualize and prove protein interactions in a cellular context.
Twelve tips for developing and sustaining a programme of student selected components

Background: Student selected components (SSCs) represent a significant component of medical curricula in the UK and a new approach in medical education. Despite the prominence given to SSCs by the General Medical Council in each of its seminal papers regarding undergraduate medical education, there remains a diverse view of the purpose, outcomes, structure and assessment of SSCs. Many Schools have adopted their own perspective of SSCs and created different but often innovative courses. Aims: This article brings together the Scottish Medical Schools and their experience in organising SSCs, highlights some of the challenges and offers possible solutions to some of the difficulties encountered. Method: The SSC Director from each of the Scottish medical schools each contributed their own ‘12 Tips’. From these a consensus was achieved. Results: Even though the Scottish medical schools have a wide range of curriculum and timetable formats, there was a great deal of agreement in the challenges and problems encountered in their SSC programmes, as expressed through these 12 Tips. Conclusion: There is much diversity in SSC programmes at different medical schools, although there is also much commonality in the challenges that arise. We hope that this paper will promote thought and discussion amongst those involved, and be useful to those involved in curriculum and programme development and also to those new to medical education.
Expression and Mutation Analysis of Her2 in Head and Neck Squamous Cell Carcinoma

ABSTRACT We analyzed mutation and expression status of human epidermal growth factor receptor 2 (Her2) in head and neck squamous cell carcinoma (HNSCC) using single strand conformation polymorphism (SSCP) mutation analysis and immunohistochemistry (IHC). Mutations were absent in all 85 cases. Out of 57 cases available for IHC, Her2 protein expression was negative (0) in 40 tumors (70%). Seventeen tumors (29.8%) expressed Her2, among these 13 tumors (22.8%) showed a weak (+1) expression and 4 (7%) showed a moderate expression (+2), none showed a strong (+3) expression. There was not a significant association between expression and any of the patients’ clinical variables or prognosis. Our results suggest that Her2 may not be useful as a molecular target in HNSCC.
Éthique de la recherche avec les peuples autochtones. Qu’en pensent les principaux intéressés ?

La transition vers la recherche participative et vers un plus grand controle des peuples autochtones sur les orientations et la realisation des travaux de recherche a amene les agences canadiennes de financement de la recherche a integrer un chapitre consacre a la recherche avec les peuples autochtones a leur Enonce de politique des trois conseils : ethique de la recherche avec des etres humains. Nous resumons ici les propos des participants a deux seminaires sur l’ethique de la recherche avec les Peuples autochtones tenus en 2009 et en 2011 a l’Universite du Quebec en Abitibi-Temiscamingue, afin de determiner comment les principes de respect des personnes, de preoccupation pour le bien-etre et de justice devraient etre appliques concretement, sur le terrain. A l’apparent paradoxe selon lequel il y aurait a la fois trop et pas assez de recherches avec les Peuples autochtones nous opposons un constat de manque de recherches pertinentes, du point de vue autochtone.
Evaluation of the Minolta bilirubin meter as a screening device in white and black infants.

The only currently available method for the determination of the serum bilirubin concentration is blood sampling followed by analysis in the laboratory. Yamanouchi et al1 have presented data concerning a two-wavelength (460, 555 nm) transcutaneous bilirubin meter (Minolta Camera Company, Ltd). They found a significant correlation with serum bilirubin concentrations of 0 to 20 mg/100 ml in full-term Oriental infants. However, there are no published data concerning the use of the instrument in white or black full-term or premature infants. The purpose of this study was to evaluate the Minolta transcutaneous bilirubin meter in these populations. MATERIALS AND METHODS During a two-month period, measurements were taken with the Minolta transcutaneous bilirubin meter on any infant for whom a serum bilirubin determination had been ordered.
EFECTOS ESCOLARES DE FACTORES SOCIO-AFECTIVOS. UN ESTUDIO MULTINIVEL PARA IBEROAMÉRICA

One of the classic themes of study in the research of school effectiveness is to estimate the magnitude of school effects, i.e., the weight of school on student performance. However, most studies have focused on the study of school effects for cognitive product variables, such as mathematics or language performance, ignoring socio-emotional variables such as self-concept, student attitude or behavior. This article shows the results of a study whose objectives are to estimate the magnitude of the effect of school, classroom and country for four socio-emotional variables, and to analyse the consistency of the effects among these. We carried out a multilevel study of 4 levels of analysis with data from 5,600 students from Spain and 8 Latin American countries, in 248 classrooms in 98 schools. The results suggest that school effects are very low for the socio-effective product variables and that there is little consistency among them.
Baicalin Alleviates Lipopolysaccharide-Induced Liver Inflammation in Chicken by Suppressing TLR4-Mediated NF-κB Pathway

As a kind of potent stimulus, lipopolysaccharide (LPS) has the ability to cause cell damage by activating toll-like receptor(TLR)4, then nuclear factor kappa B (NF-κB) translocates into the nucleus and changes the expression of related inflammatory genes. Baicalin is extracted from Radix Scutellariae, which possesses anti-inflammation, antioxidant and antibacterial properties. However, the effects of it on LPS-induced liver inflammation have not been fully elucidated. This study aims to investigate the anti-inflammatory effects of Baicalin on the LPS-induced liver inflammation and its underlying molecular mechanisms in chicken. The results of histopathological changes, serum biochemical analysis, NO levels and myeloperoxidase activity showed that Baicalin pretreatment ameliorated LPS-induced liver inflammation. ELISA and qPCR assays showed that Baicalin dose-dependently suppressed the production of IL-1β, IL-6, and TNF-α. Furthermore, the mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were significantly decreased by Baicalin. TLR4 is an important sensor in LPS infection. Molecular studies showed that the expression of TLR4 was inhibited by Baicalin pretreatment. In addition, Baicalin pretreatment inhibited NF-kB signaling pathway activation. All results demonstrated the protective effects of Baicalin pretreatment against LPS-induced liver inflammation in chicken via negative regulation of inflammatory mediators through the down-regulation of TLR4 expression and the inhibition of NF-kB activation.
Girdlestone resection arthroplasty – A limb salvage procedure after failed hip surgery in a rural setup

Background: Excision arthroplasty of the hip was first documented in surgical practice over a century ago. However, it was made popular in 1943 by Gathorne Girdlestone who used the technique for the treatment for septic arthritis of the hip.1 Thus his name has been synonymous with the procedure, which is currently used to address problems arising from failed hip surgery including peri-prosthetic infections or recurrently dislocating prostheses. Materials & Methods: Between 2001 and 2016, the case notes and theatre charts of 57 patients who had undergone Girdle stone resection arthroplasty were reviewed retrospectively. The indications were sepsis after a hip arthroplasty in 35 cases, aseptic loosening of the prosthesis in 5, recurrent dislocation of a hip arthroplasty in 2 and failed internal fixation of a femoral neck fracture in 15. The final subjective outcome was evaluated by questionnaire performed by a single assessor. Subjective assessment was recorded in terms of pain, leg length discrepancy, functional activities, infection control, use of a walking aid, walking ability and overall satisfaction status according to Harris Hip Scoring Questionnaire. Results: After recovery from operative intervention 33% of patients experienced mild pain to no pain at all and were considered to have adequate pain control. Finally, 79.16% were happy with the outcome, 12.5% of patients were unhappy with the long-term result, and 8.33% were indifferent. Conclusions: This study found that the Girdlestone resection arthroplasty was a viable option to salvage irreversibly failed operated hip fractures in medically and economically suboptimal patients in rural setup.
HIDRELÉTRICAS NO RIO MADEIRA: REFLEXÕES SOBRE IMPACTOS AMBIENTAIS E SOCIAIS

A hidroeletricidade e a principal tecnologia na oferta de eletricidade no Brasil, sob o argumento de que ha vantagens comparativas. Estudos ja demonstraram que os problemas sociais e ambientais sao obstaculos, e que ha alternativas tecnologicas viaveis. Esta em andamento a construcao de usinas hidreletricas na Amazonia, apesar da destruicao da biodiversidade local e danos as estruturas sociais que causam
Microfoundations of Internal and External Absorptive Capacity Routines

The 20 years following the introduction of the seminal construct of absorptive capacity (AC) by Cohen and Levinthal (Cohen, W. M., D. A. Levinthal. 1989. Innovation and learning: The two faces of RD Cohen, W. M., D. A. Levinthal. 1990. Absorptive capacity: A new perspective on learning and innovation. Admin. Sci. Quart.35(1) 128--152) have seen the proliferation of a vast literature citing the AC construct in over 10,000 published papers, chapters, and books, and interpreting it or applying it in many areas of organization science research, including organization theory, strategic management, and economics. However, with very few exceptions, the specific organizational routines and processes that constitute AC capabilities remain a black box. In this paper, we propose a routine-based model of AC as a first step toward the operationalization of the AC construct. Our intent is to direct attention to the importance of balancing internal knowledge creating processes with the identification, acquisition, and assimilation of new knowledge originating in the external environment. We decompose the construct of AC into two components, internal and external AC capabilities, and identify the configuration of metaroutines underlying these two components. These higher-level routines are expressed within organizations by configurations of empirically observable practiced routines that are idiosyncratic and firm specific. Therefore, we conceptualize metaroutines as the foundations of practiced routines. The ability of organizations to discover and implement complementarities between AC routines may explain why some firms are successful early adopters and most firms are imitators. Success as an early adopter of a new management practice or an innovation is expected to depend on the extent to which an organization evolves, adapts, and implements the configuration of its internal and external absorptive capacity routines.
Accurate model for time-dependent dielectric breakdown of high-k metal gate stacks

Time-dependent dielectric breakdown (TDDB) in high-k (HK) dielectric stacks is characterized by short breakdown times and shallow Weibull slopes. In this work, these observations are explained by a percolation model with different defect generation rates in the HK layer and interfacial SiOx layer that form the stack. The difference in defect generation rate impacts the statistics of breakdown of the stack and bimodal distributions are obtained with a transition from a shallow to steep Weibull slope for large areas. It is shown that for a HK layer with a low initial defect density, long breakdown times and steep Weibull slopes are obtained for typical product areas, mitigating TDDB as a reliability show-stopper for HK dielectrics.
User experience rating scales with 7, 11, or 101 points: does it matter?

There is a large body of work on the topic of the optimal number of response options to use in multipoint items. The takeaways from the literature are not completely consistent, most likely due to variation in measurement contexts (e.g., clinical, market research, psychology) and optimization criteria (e.g., reliability, validity, sensitivity, ease-of-use). There is also considerable research literature on visual analog scales (VAS), which are endpoint-anchored lines on which respondents place a mark to provide a rating. Typically, a VAS is a 10-cm line with the marked position converted to a 101-point scale (0-100). Multipoint rating items are widely employed in user experience (UX) research. The use of the VAS, on the other hand, is relatively rare. It seems possible that the continuous structure of the VAS could offer some measurement advantages. Our objective for this study was to compare psychometric properties of individual items and multiitem questionnaires using 7- and 11-point Likert-type agreement items and the VAS in the context of UX research. Some characteristics (e.g., means and correlations) of the VAS were different from the Likert-style (7- and 11-point items), so the VAS does not appear to be interchangeable with the Likert-style items. There were no differences in the classical psychometric properties of reliability and concurrent validity. Thus, we did not find any particular measurement advantage associated with the use of 7-point, 11-point, or VAS items. With regard to measurement properties, it doesn't seem to matter (but the literature suggests multipoint items are easier to use).
Multigenerational Perceptions of Mental Health Services of Deaf Adults in Florida

The objective of the study was to better understand the perceptions and needs of multigenerational Deaf adults related to mental health services. A survey sampled participants who were between 20 and 85 years old and Deaf. Questions were developed to identify the perspectives of Deaf adults related to the availability of mental health services, preferences for these services, and current utilization of services. Participants were grouped into age (years) categories: young adult (18–34), middle adult (35–54), older adult (55–65), and oldest (66–). Category response trends were examined using chi-square analysis. The analysis showed significant differences in the preferences and utilization of mental health care. These data also suggested preferences for service delivery. These data indicate areas of importance related to the development of programs and services for Deaf adults and to indicate where funding for services would be best utilized.
Normative Data for the Multiple Affect Adjective Check List

The development of the Multiple Affect Adjective Check List (MAACL) has been described previously ( 1 ) . Table 1 presents data for 2 normal samples and 5 psychiatric samples. The job applicant sample was drawn from a population of 1200 job applicants at the Indiana University Medical Center personnel office. These 200 Ss were stratified by age, sex, and education to match the census distribution of these variables. The college students were obtained from introductory psychology classes at Adelphi College in New York. Two samples of psychiatric patients were obtained from Larue D. Carter Memorial Hospital in Indiana; another sample of patients was tested at Central Islip Hospital in New York, and 2 samples of V.A. patients were obtained from the Mr. Alto V.A. in Washington, D. C. , and the St. Cloud V.A. Hospital in Minnesota. All patients, except those from St. Cloud V.A. , were tested within a few weeks after admission. The St. Cloud patients were part of a drug srudy and were rested after the completion of drug treatment. The Mr. Alco V.A. patients were retested with the MAACL 8 days after the first test. Significant differences berween normal and patient samples are indicated.
The role of antibody in the treatment of acute herpes simplex virus disease and in the pathogenesis of latency in newborn guinea pigs.

To the EditorIn their study of herpes simplex virus (HSV) antibody in the treatment of experimental neonatal HSV infection in guinea pigs, Bravo et al. [1] emphasized the potential therapeutic benefit of antibody in the treatment of acute disease. Multiple sites of infection were monitored, and infection was decreased after administration of antibody; however, there was no effect on HSV recurrences, suggesting no effect on HSV latency. A colleague and I previously reported similar data on decreased mortality of newborn guinea pigs treated with antibody to HSV, but we noted decreased latency [2]. In that study, newborn guinea pigs were inoculated intraperitoneally (ip) with antibody to HSV or were born of females recently immunized against HSV. Mortality was similarly decreased in both groups. Latency was decreased in the ip-immunized group and was prevented in the newborns born of immunized females. Since the latter group of newborns had higher antibody titers than the former, a dose-dependent effect was suggested (although the role of other transplacental factors could not be ruled out). The suggestion by Bravo et al. [1] that decreased latency did not result from antibody treatment, although decreased HSV replication presumably did occur, is in keeping with recent reports that HSV replication is not necessary for the establishment of latency [3-7]. Two points can be raised, however, in consideration of these observations: First, these data were obtained with replicationdefective HSV mutants, not wild type HSV; second, decreased wild type HSV replication has been correlated with decreased latency. Decreased latency was measured by decreased HSV reactivation [2, 8] or decreased expression of HSV latency-associated
The dream in contemporary psychiatry.

OBJECTIVE This article offers selective reviews of cogent sectors of research regarding the dream in contemporary psychiatry. METHOD First, the author discusses relatively recent research (1953-1999) on the neurobiology and clinical psychophysiology of dreaming sleep; second, he reviews experimental cognitive neuroscientific studies of perception, emotion, and memory and the putative interrelationships among them in generating dream imagery; and third, he interprets psychoanalytic studies (1900-1999) on related aspects of dreams and the dream process. RESULTS Exploration for interrelationships among information from these three areas entails discussion of the mind/brain problem. These considerations illuminate some of the logical and interpretive dilemmas that enter into debates about Freud's theory of the dream. CONCLUSIONS The author proposes a preliminary psychobiologic concept of the dream process and discusses, in light of the foregoing considerations, the importance of collaborative research for developing a realistic perspective concerning the proper place of the dream in contemporary psychiatry.
Representações sociais sobre pessoas com HIV/AIDS entre enfermeiros: uma análise estrutural e de zona muda

Trata-se de um estudo quanti-qualitativo, descritivo, objetivando: descrever e analisar o conteudo e a estrutura das representacoes sociais em torno da pessoa com HIV/AIDS entre enfermeiros. Foram 30 sujeitos/enfermeiros de um hospital universitario da capital do Rio de Janeiro/Brasil. Os dados foram coletados por Questionario de Caracterizacao e pela tecnica de Mise en Cause . Os resultados indicaram um processo de mudanca representacional sobre o HIV/AIDS e seus portadores, considerando o deslocamento do termo morte da centralidade para a periferia da representacao; e a incorporacao da cognicao tratamento naquela posicao. Foi corroborada a existencia de uma possivel zona muda na representacao, composta por elementos de carater contranormativo. Concluiu-se que as tecnicas empregadas contribuiram para a melhor compreensao do conteudo e da estrutura das representacoes em foco, sendo necessario maior investimento em estudos sobre o papel da normatividade social neste processo.
Deep learning for extracting protein-protein interactions from biomedical literature

State-of-the-art methods for protein-protein interaction (PPI) extraction are primarily feature-based or kernel-based by leveraging lexical and syntactic information. But how to incorporate such knowledge in the recent deep learning methods remains an open question. In this paper, we propose a multichannel dependency-based convolutional neural network model (McDepCNN). It applies one channel to the embedding vector of each word in the sentence, and another channel to the embedding vector of the head of the corresponding word. Therefore, the model can use richer information obtained from different channels. Experiments on two public benchmarking datasets, AIMed and BioInfer, demonstrate that McDepCNN provides up to 6% F1-score improvement over rich feature-based methods and single-kernel methods. In addition, McDepCNN achieves 24.4% relative improvement in F1-score over the state-of-the-art methods on cross-corpus evaluation and 12% improvement in F1-score over kernel-based methods on “difficult” instances. These results suggest that McDepCNN generalizes more easily over different corpora, and is capable of capturing long distance features in the sentences.
Polymorphisms in Inflammasome' Genes and Susceptibility to HIV-1 Infection

Abstract:The involvement of inflammasome genes in the susceptibility to HIV-1 infection was investigated. Twelve single nucleotide polymorphisms within NLRP1, NLRP3, NLRC4, CARD8, CASP1, and IL1B genes were analyzed in 150 HIV-1–infected Brazilian subjects and 158 healthy controls. The 2 polymorphisms rs10754558 in NLRP3 and rs1143634 in IL1B were significantly associated to the HIV-1 infection. These findings supported the previously hypothesized involvement of NALP3-inflammasome in HIV-1 pathogenesis, underlining once more the key role of inflammation and innate immunity in the susceptibility to HIV-1 infection.