Miracle-dz/UniFAR-FTUs huggingface dataset:
High Concentrations of Polyelectrolyte Complex Nanoparticles Decrease Activity of Osteoclasts

Fracture treatment in osteoporotic patients is still challenging. Osteoporosis emerges when there is an imbalance between bone formation and resorption in favor of resorption by osteoclasts. Thus, new implant materials for osteoporotic fracture treatment should promote bone formation and reduce bone resorption. Nanoparticles can serve as drug delivery systems for growth factors like Brain-Derived Neurotrophic Factor (BDNF), which stimulated osteoblast differentiation. Therefore, polyelectrolyte complex nanoparticles (PEC-NPs) consisting of poly(l-lysine) (PLL) and cellulose sulfate (CS), with or without addition of BDNF, were used to analyze their effect on osteoclasts in vitro. Live cell images showed that osteoclast numbers decreased after application of high PLL/CS PEC-NPs concentrations independent of whether BDNF was added or not. Real-time RT-PCR revealed that relative mRNA expression of cathepsin K and calcitonin receptor significantly declined after incubation of osteoclasts with high concentrations of PLL/CS PEC-NPs. Furthermore, Enzyme-Linked Immunosorbent Assay indicated that tartrate-resistant acidic phosphatase 5b activity was significantly reduced in the presence of high PLL/CS PEC-NPs concentrations. Consistent with these results, the pit formation analysis showed that less hydroxyapatite was resorbed by osteoclasts after incubation with high concentrations of PLL/CS PEC-NPs. BDNF had no influence on osteoclasts. We conclude that highly concentrated PLL/CS PEC-NPs dosages decreased osteoclastogenesis and osteoclasts activity. Moreover, BDNF might be a promising growth factor for osteoporotic fracture treatment since it did not increase osteoclast activity.
A difficulty predictor for perceptual category learning

Predicting human performance in perceptual categorization tasks in which category membership is determined by similarity has been historically difficult. This article proposes a novel biologically motivated difficulty measure that can be generalized across stimulus types and category structures. The new measure is compared to 12 previously proposed measures on four extensive data sets that each included multiple conditions that varied in difficulty. The studies were highly diverse and included experiments with both continuous- and binary-valued stimulus dimensions, a variety of different stimulus types, and both linearly and nonlinearly separable categories. Across these four applications, the new measure was the most successful at predicting the observed rank ordering of conditions by difficulty, and it was also the most accurate at predicting the numerical values of the mean error rates in each condition.
Psychological Effects of Abuse, Partner Pressure, and Alcohol: The Roles of in-the-Moment Condom Negotiation Efficacy and Condom-Decision Abdication on Women’s Intentions to Engage in Condomless Sex

Women’s experiences of intimate partner violence (IPV) from their male partners can include psychological, physical, and sexual control and abuse. The psychological effects of abuse (PEA) include terror, shame, and loss of power and control in relationships. While women’s experiences of IPV are associated with decreased condom use, limited research has examined the impact of PEA on women’s condom use. Intoxicated (breath alcohol content [BrAC] = .10%) versus sober women were evaluated to test the hypothesis that PEA would interact with intoxication and scenario-context partner pressure to forgo condom use and be associated with intentions to engage in condomless sex. After beverage administration, community women (N = 405) projected themselves into a computerized scenario depicting a male partner exerting high or low pressure for condomless sex. In-the-moment condom negotiation self-efficacy and condom-decision abdication—letting the man decide on condom use—were assessed. Path analysis examined the direct and indirect effects of PEA, alcohol, and partner pressure conditions on condomless sex intentions. PEA increased condomless sex intentions indirectly through decreased condom negotiation self-efficacy. Intoxication increased condomless sex intentions indirectly through decreased condom negotiation self-efficacy and increased condom-decision abdication. Intoxicated women in the low pressure condition were more likely to abdicate the condom decision than women in the high pressure condition. Women who have experienced greater PEA may benefit from interventions focusing on how condom negotiation self-efficacy, condom-decision abdication, and intoxication influence sexual decision-making.
Pregnancy-Related Mortality and Severe Maternal Morbidity in Rural Appalachia: Established Risks and the Need to Know More.

PURPOSE The Centers for Disease Control and Prevention (CDC) and the American College of Obstetrics and Gynecology have called for researchers to further elucidate medical and social determinants of pregnancy-related death and severe maternal morbidity. This report begins to answer this call in the context of rural Appalachia. METHODS This report identifies risk factors exposing women in rural Appalachia to pregnancy-related death and severe maternal morbidity. We also use CDC WONDER data to illustrate rural-urban differences in pregnancy-related death. FINDINGS Rural women nationally die of pregnancy-related causes at a greater rate than urban women. It is unknown how rurality specifically influences pregnancy-related death, but rural women more often embody multiple risk factors associated with negative maternal outcomes. Established risk factors, including high rates of chronic illness and substance abuse, place rural women at risk for severe maternal morbidity and pregnancy-related mortality. These women may also lack the resources to mitigate these risks, including access to high-risk obstetric care. NEXT STEPS To address these issues and the concerning lack of data, we propose 4 directions for future study: (1) a determination of the prevalence of pregnancy-related death and severe maternal morbidity in this population; (2) an examination of how rural women utilize existing pre- and perinatal resources; (3) better validation concerning surveillance methods of pregnancy-related death and severe maternal morbidity in rural areas; and (4) an exploratory qualitative study of rural women and health care providers.
Objective assessment of attention‐deficit/hyperactivity disorder in older adults compared with controls using the QbTest

Attention‐deficit/hyperactivity disorder (ADHD) persists into old age, with prevalence rates of 2.8% to 3.3% in adults over 60 years of age. Most diagnostic assessment tools are not validated for older adults. The Quantified behavioral Test (QbTest) is an objective assessment for the core symptoms of ADHD and is validated for children and younger adults. We investigated whether the QbTest can be used to differentiate between older adults with ADHD and healthy controls.
Overexpression of X Intrinsic Protein 1;1 in Nicotiana tabacum and Arabidopsis reduces boron allocation to shoot sink tissues

Abstract Major Intrinsic Proteins (MIP) are a family of channels facilitating the diffusion of water and/or small solutes across cellular membranes. X Intrinsic Proteins (XIP) form the least characterized MIP subfamily in vascular plants. XIPs are mostly impermeable to water but facilitate the diffusion of hydrogen peroxide, urea and boric acid when expressed in heterologous expression systems. However, their transport capabilities in planta and their impact on plant physiology are still unknown. Here, we demonstrated that overexpression of NtXIP1;1 in Nicotiana tabacum by the En2pPMA4 or the 35S CaMV promoter and in Arabidopsis, which does not contain any XIP gene, by the 35S CaMV promoter, resulted in boron (B)‐deficiency symptoms such as death of the shoot apical meristem, infertile flowers, and puckered leaves. Leaf B concentrations in symptomatic tissues and B xylem sap concentrations were lower in the overexpressors than in control plants. Importantly, expression of NtXIP1;1 under the control of the AtNIP5;1 promoter complemented the B deficiency phenotype of the Atnip5;1 knockout mutant, defining its ability to act as a boric acid channel in planta. Protein quantification analysis revealed that NtXIP1;1 was predominantly expressed in young B‐demanding tissues and induced under B‐deficient conditions. Our results strongly suggest that NtXIP1;1 plays a role in B homeostasis and its tissue‐specific expression critically contributes to the distribution of B within tobacco.
Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer

Objective MicroRNA (miR)-421 plays a key role in cancer progression. It has been reported that circulating miR-421may be a potential tumor marker for the diagnosis of several cancers. However, the role of miR-421 in plasma as a potential biomarker in the diagnosis of precancerous gastric lesions (Pre) and early-stage gastric cancer (GC) remains poorly understood. In this study, we investigated miR-421 in plasma as a novel potential biomarker for the detection of precancerous gastric lesions and early-stage (GC). Materials & Methods The miRNA content was determined by quantitative real-time polymerase chain reaction (qRT-PCR). MiR-421 content in all subjects was normalized by endogenous miRNA (miR-16). The diagnostic value of miR-421 for Pre and GC was assessed by comparing receiver operating characteristic (ROC) analysis with traditional tumor markers, including CEA, CA125, CA153, CA211 and CA50. The correlation between the expression of miR-421 and the pathological characteristics of Pre and GC was analyzed. Results Elevated expression of miR-421 in plasma can robustly distinguish the normal population from Pre and GC cases, especially in the early stages of gastric cancer cases (all p < 0.05). The ROC analyses showed that the area under the ROC curve (AUC), sensitivity, accuracy and Youden index of miR-421 were superior to traditional tumor markers (CEA, CA125, CA153, CA211, and CA50) in GC diagnosis, while its specificity was higher than CEA, CA153 and CA50 (all p < 0.05). MiR-421 in plasma had higher AUC value than AFP, CA153, CA211 and CA50 in the diagnosis of Pre (all p < 0.05), while specificity, accuracy and Youden index of miR-421 was only lower than CA211. The efficiency of miR-421 in the diagnosis of GC was significantly higher than that of CA211 and CA50, and it was significantly higher than CA153, CA211 and CA50 in the diagnosis of Pre (all p < 0.05). In addition, up-regulation of miR-421 occurred initially in precancerous gastric lesions as well as in the early stage of GC. Conclusions Overexpression of plasma miR-421 is a novel biomarker for the detection of precancerous lesions and early gastric cancer.
Legionella Pneumonia Following the Heavy Rain Event of July 2018 in Japan

We herein report the case of a 62-year-old man diagnosed with Legionella pneumonia while engaged in recovery work in a flooded area after the Heavy Rain Event of July 2018 in Japan. The patient was intubated and maintained on mechanical ventilation and continuous hemodiafiltration. He was also administered antimicrobial therapy with ciprofloxacin and azithromycin. After 53 days in the hospital, he was discharged. It is important to recognize the risk of Legionella infection and to take measures to prevent it during recovery work that involves exposure to water and soil after a flood disaster.
Utility of Circulating Cell-Free RNA Analysis for the Characterization of Global Transcriptome Profiles of Multiple Myeloma Patients

In this study, we evaluated the utility of extracellular RNA (exRNA) derived from the plasma of multiple myeloma (MM) patients for whole transcriptome characterization. exRNA from 10 healthy controls (HC), five newly diagnosed (NDMM), and 12 relapsed and refractory (RRMM) MM patients were analyzed and compared. We showed that ~45% of the exRNA genes were protein-coding genes and ~85% of the identified genes were covered >70%. Compared to HC, we identified 632 differentially expressed genes (DEGs) in MM patients, of which 26 were common to NDMM and RRMM. We further identified 54 and 191 genes specific to NDMM and RRMM, respectively, and these included potential biomarkers such as LINC00863, MIR6754, CHRNE, ITPKA, and RGS18 in NDMM, and LINC00462, PPBP, RPL5, IER3, and MIR425 in RRMM, that were subsequently validated using droplet digital PCR. Moreover, single nucleotide polymorphisms and small indels were identified in the exRNA, including mucin family genes that demonstrated different rates of mutations between NDMM and RRMM. This is the first whole transcriptome study of exRNA in hematological malignancy and has provided the basis for the utilization of exRNA to enhance our understanding of the MM biology and to identify potential biomarkers relevant to the diagnosis and prognosis of MM patients.
High Impact and Highly Cited Peer-Reviewed Journal Article Publications by Canadian Occupational Therapy Authors: A Bibliometric Analysis

Abstract A bibliometric analysis of high impact and highly cited peer-reviewed literature published between 1992 and 2016 by Canadian occupational therapy authors that were included in the Science Citation Index-Expanded (SCI-E) or Social Sciences Citation Index (SSCI) was completed. To complete the analysis, journal article titles, abstracts, author details, and keywords were searched. A second-filter identified articles where the first or corresponding author had a Canadian affiliation and occupational therapy qualification. The total number of times an article was cited since its initial publication and during 2016 in Web of Science Core Collection was recorded. A total of 919 retrieved articles met the inclusion criteria with 18 articles having 5 or more citations during 2016 alone and another 34 articles having 50 or more citations since their initial publication date. The top three journals where high impact and highly-cited articles were published were Archives of Physical Medicine and Rehabilitation, Disability and Rehabilitation, and American Journal of Occupational Therapy. The three institutions that generated the largest number of high impact and highly cited articles were McGill University, University of Toronto, and University of British Columbia. Therefore, as of 2016, Canadian occupational therapy authors published 18 high impact and 34 highly cited articles.
Automated lung nodule detection and classification based on multiple classifiers voting

Lung cancer is the most common cause of cancer‐related death globally. Currently, lung nodule detection and classification are performed by radiologist‐assisted computer‐aided diagnosis systems. However, emerged artificially intelligent techniques such as neural network, support vector machine, and HMM have improved the detection and classification process of cancer in any part of the human body. Such automated methods and their possible combinations could be used to assist radiologists at early detection of lung nodules that could reduce treatment cost, death rate. Literature reveals that classification based on voting of classifiers exhibited better performance in the detection and classification process. Accordingly, this article presents an automated approach for lung nodule detection and classification that consists of multiple steps including lesion enhancement, segmentation, and features extraction from each candidate's lesion. Moreover, multiple classifiers logistic regression, multilayer perceptron, and voted perceptron are tested for the lung nodule classification using k‐fold cross‐validation process. The proposed approach is evaluated on the publically available Lung Image Database Consortium benchmark data set. Based on the performance evaluation, it is observed that the proposed method performed better in the stateof the art and achieved an overall accuracy rate of 100%.
A DNA Prime Immuno-Potentiates a Modified Live Vaccine against the Porcine Reproductive and Respiratory Syndrome Virus but Does Not Improve Heterologous Protection

The porcine reproductive and respiratory syndrome virus (PRRSV), an RNA virus inducing abortion in sows and respiratory disease in young pigs, is a leading infectious cause of economic losses in the swine industry. Modified live vaccines (MLVs) help in controlling the disease, but their efficacy is often compromised by the high genetic diversity of circulating viruses, leading to vaccine escape variants in the field. In this study, we hypothesized that a DNA prime with naked plasmids encoding PRRSV antigens containing conserved T-cell epitopes may improve the protection of MLV against a heterologous challenge. Plasmids were delivered with surface electroporation or needle-free jet injection and European strain-derived PRRSV antigens were targeted or not to the dendritic cell receptor XCR1. Compared to MLV-alone, the DNA-MLV prime- boost regimen slightly improved the IFNγ T-cell response, and substantially increased the antibody response against envelope motives and the nucleoprotein N. The XCR1-targeting of N significantly improved the anti-N specific antibody response. Despite this immuno-potentiation, the DNA-MLV regimen did not further decrease the serum viral load or the nasal viral shedding of the challenge strain over MLV-alone. Finally, the heterologous protection, achieved in absence of detectable effective neutralizing antibodies, was not correlated to the measured antibody or to the IFNγ T-cell response. Therefore, immune correlates of protection remain to be identified and represent an important gap of knowledge in PRRSV vaccinology. This study importantly shows that a naked DNA prime immuno-potentiates an MLV, more on the B than on the IFNγ T-cell response side, and has to be further improved to reach cross-protection.
Transforming youth mental health services in a large urban centre: ACCESS Open Minds Edmonton

This paper outlines the transformation of youth mental health services in Edmonton, Alberta, a large city in Western Canada. We describe the processes and challenges involved in restructuring how services and care are delivered to youth (11‐25 years old) with mental health needs based on the objectives of the pan‐Canadian ACCESS Open Minds network.
Validation of the Arabic version of vocal tract discomfort scale

Abstract Aim: To explore the internal consistency, criterion validity, and discriminative validity of the Arabic version of the vocal tract discomfort scale (VTDS-Arab); and to establish cut-off scores for the VTDS-Arab. Methodology: An overall of 202 participants (31 patients with voice disorders of organic and functional causes, and 171 healthy subjects, mean age 25.3 ± 6.7) were recruited in this study. Participants completed the VTDS-Arab scale and the Arabic version of the voice handicap index (VHI-Arab). Results: The VTDS-Arab had a positive internal consistency confirmed by Cronbach’s α = 0.91. The criterion validity of the VTDS-Arab was confirmed by the strong positive correlation between the total scores of the VTDS-Arab and the VHI-Arab (r = 0.64, p < .001). Patients scored higher than the healthy subjects in all the subscales of the VTDS-Arab (p < .001) which confirmed the discriminative validity of the VTDS-Arab. Age and gender were not found to affect the scores of the VTDS as confirmed by Pearson r test (r = 0.02, p = .80) and insignificant p values of Levene’s test (F1, 200 = 0.43, p = .51), respectively, which supports the validity of the VTDS-Arab. The study posited interpretations for the ranges of scores of the VTDS-Arab based, where the cut-off score for the VTDS-Arab total was 26. Conclusion: The VTDS-Arab is a reliable and a clinically valid tool that can be used in the evaluation of voice disorders alongside other subjective and objective tools of evaluation.
Adipose-Derived Mesenchymal Stem Cells: A Promising Tool in the Treatment of Musculoskeletal Diseases

Chronic musculoskeletal (MSK) pain is one of the most common medical complaints worldwide and musculoskeletal injuries have an enormous social and economical impact. Current pharmacological and surgical treatments aim to relief pain and restore function; however, unsatiscactory outcomes are commonly reported. In order to find an accurate treatment to such pathologies, over the last years, there has been a significantly increasing interest in cellular therapies, such as adipose-derived mesenchymal stem cells (AMSCs). These cells represent a relatively new strategy in regenerative medicine, with many potential applications, especially regarding MSK disorders, and preclinical and clinical studies have demonstrated their efficacy in muscle, tendon, bone and cartilage regeneration. Nevertheless, several worries about their safety and side effects at long-term remain unsolved. This article aims to review the current state of AMSCs therapy in the treatment of several MSK diseases and their clinical applications in veterinary and human medicine.
Challenging treatment of in-stent restenosis in a coronary bifurcation by implantation of a bioresorbable scaffold under optical coherence tomography guidance

A 67-year-old male patient with stable angina, hypertension and hypercholesterolemia who underwent bare metal stent (BMS) implantation in the distal right coronary artery (RCA) (Azule 3 × 9 mm) and everolimus-eluting stent (EES) implantation in the first diagonal branch (D1) (Xience 2.25 × 18 mm) and in the proximal circumflex branch (LCx) (Xience 3 × 28 mm). One year subsequent to the precedure the patient was readmitted for relapse of the angina Canadian Cardiovascular Society scale II, exhibiting a positive exercise test. The coronary angiography showed a distal-edge in-stent restenosis (ISR) in the distal RCA, extending to the posterior descending artery (PDA), Medina 110 bifurcation (Fig. 1A). Optical coherence tomography (OCT) showed predominantly fibrolipidic restenotic tissue, with minimal lumen area (MLA) 1.95 mm, minimal lumen diameter (MLD) 1.57 mm, proximal reference vessel diameter (RVD) 3.1 mm, distal RVD 2.75 mm and lesion length 21.2 mm (Fig. 1B, C). Optical coherence tomography-guided implantation of a bioresorbable scaffold (BRS) to treat the bifurcation ISR was performed through a radial approach, using a 6 french guiding-catheter. Guidewires were placed in the PDA and in the posterolateral artery (PLA), in order to protect the side branch in case of an eventual occlusion. Predilation 1:1 with a non-compliant (NC) balloon 3.0 × 18 mm (16 atm) was performed until the balloon was completely expanded in angiography. A second OCT run verified fragmentation of restenotic tissue and sufficient luminal gain to ensure adequate scaffold expansion. A poly-lactide BRS (ABSORB 3 × 28 mm) was then slowly deployed at 12 atm, holding pressure for 60 s. Proximaloptimalization-technique with an NC-balloon 3.25 × 15 mm (16 atm) was then performed by placing the proximal edge of the distal marker of the balloon at the carina of the PDA-PLA bifurcation, with an optimal angiographic result (Fig. 1D). A final OCT pullback showed optimal apposition and expansion (MLA 5.3 mm/MLD 2.6 mm; Fig. 1E), structural integrity of the device and clear access to the PLA side branch through the scaffold struts (Fig. 1F). Three-month follow-up documented an optimal clinical and angiographical result (Suppl. Video 1). Poly-lactide BRS are supposed to resorb completely [1–5], depending on the specific device and on patient/local conditions. The resorption restores vasomotion and eventually normal endothelial INTERVENTIONAL CARDIOLOGY