Miracle-dz/UniFAR-FTUs huggingface dataset:
Gene expression analysis following hypoxia–reoxygenation in rat gastric epithelial cells using a high-density oligonucleotide array

Abstract Recent investigations have demonstrated that the signaling of hypoxia–re-oxygenation is a major contributing pathway leading to gastric mucosal injury induced by stress, non-steroidal anti-inflammatory drugs, and Helicobacter pylori. The aim of the present study was to perform a gene expression analysis on the gastric mucosal cellular response to hypoxia–reoxygenation using a high-density oligonucleotide array. Cells were subjected to hypoxia with 95% N2 and 5% CO2 at 37°C for 2 h. Reoxygenation was initiated by placing the cells in an environment of normoxia for 2 h. Total RNA was extracted, and differences in gene expression profiles between the normoxia and hypoxia–reoxygenation groups were investigated using a GeneChip of Rat Toxicology U34 array (Affymetrix). Hypoxia–reoxygenation up-regulated the stress-related genes (heat shock protein-70 [HSP-70], catalase). The enhanced expression of HSP-70 was confirmed by Western blot analysis. In conclusion, these results suggest that up-regulation of the HSP-70 gene after reoxygenation may play a role in maintaining cell survival and supporting cell function as a molecular chaperone.
A light-weight cooling vest enhances performance of athletes in the heat

During the 1990s, emphasis on the health and safety of people who exercise in hot, humid conditions increased and many organizations became aware of the need for protection against heat-related disorders. A practical, pre-cooling strategy applicable to several sporting codes, which is low cost, easy to use, light-weight and which enhances cooling of the human body prior to and following exercise, was developed and tested. Eight males and eight females participated in a maximal oxygen consumption ([Vdot]O2max) test and four trials: a control (without cooling) and wearing each of three different cooling vests (A, B, C). Vests were worn during the rest, stretch, warm-up (50% [Vdot]O2max) and recovery stages of the protocol, but not during the 30 min run (70% [Vdot]O2max). Core and skin temperatures during exercise were reduced (by approximately 0.5°C, rectal; 0.1 – 1.4°C, abdominal skin temperature) and sweat rates were lower (by approximately 10 – 23%). Endurance times for running at 95% of [Vdot]O2max were increased by up to 49 s. Perceptions of the thermal state and skin wetness showed changes to greater levels of satisfaction. Physiological and sensory responses were related to design features of the vests.
Mechanisms of Macrolide Resistance in Clinical Group B Streptococci Isolated in France

ABSTRACT Macrolide susceptibility was investigated in clinical group B streptococci obtained from neonates or pregnant women in 2000 in France. Of 490 consecutive isolates, 18% were resistant to erythromycin. The erm(B), erm(A) subclasserm(TR), and mef(A) genes were harbored by 47, 45, and 6% of these strains, respectively. Two isolates did not harborerm or mef genes.
Radio Wave Characterization and Modeling in Underground Mine Tunnels

Results are presented on wideband radio propagation measurements and statistical modeling at 2.4 GHz and 5.8 GHz in real underground mine tunnels. This peculiar type of confined environment is characterized by very rough surfaces and a frequent absence of a line-of-sight between transmitting and receiving antennas. The resulting propagation characteristics differ from those frequently encountered in more typical indoor environments such as offices and corridors. Indeed, the rms delay spread shows little or no correlation with respect to transmitter-receiver distance and, in addition, no impulse response path arrival clustering effect is observed. However, the path amplitude distribution does tend to follow a Rice distribution in the line-of-sight case, and a Rayleigh distribution otherwise.
EUVE Observations of Jupiter During the Impact of Comet Shoemaker-Levy 9

The Extreme Ultraviolet Explorer (EUVE) satellite conducted extensive observations of the jovian system before, during, and after the impact of the fragments of comet Shoemaker-Levy 9 in July 1994. About 2 to 4 hours after the impacts of several of the larger fragments, the brightness of the neutral helium (He I) resonance line at 58.4 nanometers temporarily increased by a factor of about 10. The transient 58.4-nanometer brightenings are most simply explained by resonant scattering of sunlight from the widespread high-altitude remnants of the larger impact plumes. Other possible sources of emission, such as electron impact excitation of He or radiative recombination of He+, may contribute to the observed signal.
Skeletal muscle lipid peroxidation in exercised and food-restricted rats during aging.

The effects of chronic endurance exercise and food restriction on nonenzymatic lipid peroxidation (LP) of gastrocnemius muscle during aging were studied in male, Fischer 344 rats. One set of rats aged 6 and 18 mo were assigned to an exercise group (treadmill running) or an age-matched sedentary control group. After 6 mo (at the ages of 12 and 24 mo), LP and levels of alpha-tocopherol and its oxidized form, alpha-tocopheryl quinone, were measured. The extent of LP was determined in homogenates by measuring the content of thiobarbituric acid-reactive substances. After homogenization, the muscles were immediately evaluated for basal LP and also incubated in the presence of oxidant stressors for 2 h to assess antioxidant capacity (AOC) and for 24 h to estimate total peroxidizable lipid (TPL). Basal LP was not affected by age or exercise. AOC was not affected by exercise at either age. However aging significantly decreased AOC and increased alpha-tocopheryl quinone in both sedentary and exercised groups. TPL was not affected by age, but was increased by exercise training (P less than 0.05). Another set of rats was divided into the following three groups at 3 mo of age: sedentary, fed ad libitum (S); sedentary, caloric restricted by alternate day feeding (R); and exercised by forced treadmill running (E). Two years later, when the rats were 27 mo of age, the extent of LP was assessed. (ABSTRACT TRUNCATED AT 250 WORDS)
FAM2 Regimen in Disseminated Gastric Cancer

Forty-four previously untreated patients with advanced inoperable and/or disseminated gastric carcinoma were given an i.v. combination (FAM2) chemotherapy of 5-fluorouracil, 400 mg/m2 on days 1, 2 and 3, and 21, 22 and 23; adriamycin, 40 mg/m2 on days 2 and 22; and mitomycin C, 10 mg/m2 on day 1, with a recycle on day 42 (1 cycle = 41 days). Forty patients have completed 2 cycles and are evaluable (median number of cycles 5; range 3 to 8): 26 of these achieved a partial remission, with a response rate of 65 %; 4 (10 %) gained a stable situation for 3 to 6 months, and 10 (25 %) showed progression of disease. Median duration of partial remissions was 10 months, and median survival was 15 months for responders and 5 months for nonresponders. A fall in WBC (< 2500/μl) occurred in 7 % and of platelets (< 80,000/μl) in 4.5 %. Total alopecia occurred in 20 of 40 patients and nausea and or weakness were common findings. No drug-related infection, bleeding or death was observed. Patients with advanced gastric carcinoma can derive useful palliation from FAM2 chemotherapy.
Customer Satisfaction, Product Quality and Performance of Companies

Abstract This paper presents an analysis of quality, customer satisfaction and business performance in food industry. The main objective of the research is to determine the influence of quality on customer satisfaction and on business performance and competitiveness. In particular, this paper answers the following research question: Does the quality of a product result in a satisfied customer and thereby in a well-performing business? Customer satisfaction is defined as the satisfaction of the customer with a product and the business performance as a capability to generate profit. Therefore, satisfaction was examined by the means of a survey using questionnaires, and the performance was measured by financial data. We managed to find a correlation between the main factors, although partial results were due more factors mostly statistically insignificant.
Parallel Gene Expression Monitoring using Oligonucleotide Probe Arrays of Multiple Transcripts with an Animal Model of Focal Ischemia

High density oligonucleotide arrays offer tremendous potential to study gene changes occurring in disease states. The authors described the first case of using a custom designed high density oligonucleotide probe array containing 750 genes to monitor the changes in mRNA transcript levels occurring after focal ischemia for a period of 3 hours. Permanent middle cerebral artery occlusion in the rat resulted in neuronal degeneration in the dorsolateral cortex and striatum over a time course of 24 hours. Comparing the changes in hybridization levels in the frontal and parietal cortices and the striatum, between the ipsilateral and contralateral sides of the brain using the probe arrays resulted in the up-regulation of 24 genes, which showed greater than a twofold change. Very few genes were found to be downregulated after the ischemic insult. Many of the immediate early genes (IEGs) such as c-fos, NGFI-A, NGFI-C, and Krox-20 were found to be robustly up-regulated in the three different regions studied. Other genes that were up-regulated in perifocal regions included Arc, Inhibin-β-A, and the phosphatases MKP-1 and MKP-3. The hybridization signal intensity from the probe arrays enabled quantification of many genes relative to one another, and robust changes in expression were obtained with very little interanimal variability. Furthermore, the authors were able to validate the increased expression of NGFI-C and Arc using in situ hybridization. This represented the first example of using high density oligonucleotide probe arrays in studying the expression of many genes in parallel and in discrete brain regions after focal ischemia.
Similarity of cardiovascular responses to exercise and to diencephalic stimulation.

Devices to measure left ventricular pressure, diameter and heart rate in animals with closed chests were placed on the hearts of dogs. After recovery from this operation the dogs were trained to exercise on a treadmill and the cardiovascular responses to this exercise were recorded. Stimulating electrodes were then stereotaxically placed in the diencephalon. In some dogs the electrodes were chronically implanted, and the stimulation was carried out after recovery from this second operation. In other animals stimulation was carried out immediately while they were under chloralose anesthesia. Stimulation of the H1 and H2 fields of Forel and the periventricular gray of the third ventricle resulted in cardiovascular responses similar to those which result from exercise.
Two-effective-source method for the calculation of in-air output at various source-to-detector distances in wedged fields.

A simple algorithm was developed for calculation of the in-air output at various source-to-detector distances (SDDs) on the central axis for wedged fields. In the algorithm we dealt independently with two effective sources, one for head scatter and the other for wedge scatter. Varian 2100C with 18 and 8 MV photon beams was used to examine this algorithm. The effective source position for head scatter for wedged fields was assumed to be the same as that for open fields, and the effective source position for wedge scatter was assumed to be a certain distance upstream from the physical location of the wedge. The shift of the effective source for wedge scatter, w, was found to be independent of field size. Moreover, we observed no systematic dependency of w on wedge angle or beam energy. One value, w = 5.5 cm, provided less than 1% difference in in-air outputs through the whole experimental range, i.e., 6 x 6 to 20 x 20 cm2 field size (15 x 20 cm2 for 60 degrees wedge), 15 degrees-60 degrees wedge angle, 80-130 cm SDD, and both 18 and 8 MV photon beams. This algorithm can handle the case in which use of a tertiary collimator with an external wedge makes the field size for the determination of wedge scatter different from that for head scatter. In this case, without the two-effective-source method, the maximum of 4.7% and 2.6% difference can be given by the inverse square method and one-effective-source method in a 45 degrees wedged field with 18 MV. Differences can be larger for thicker wedges. Enhanced dynamic wedge (EDW) fields were also examined. It was found that no second effective source is required for EDW fields.
Contribution of Explicit Solvent Effects to the Binding Affinity of Small‐Molecule Inhibitors in Blood Coagulation Factor Serine Proteases

The prevention of blood coagulation is important in treating thromboembolic disorders, and several serine proteases involved in the coagulation cascade have been classified as pharmaceutically relevant. Whereas structure‐based drug design has contributed to the development of some serine protease inhibitors, traditional computational methods have not been able to fully describe structure–activity relationships (SAR). Here, we study the SAR for a number of serine proteases by using a method that calculates the thermodynamic properties (enthalpy and entropy) of the water that solvates the active site. We show that the displacement of water from specific subpockets (such as S1–4 and the ester binding pocket) of the active site by the ligand can govern potency, especially for cases in which small chemical changes (i.e., a methyl group or halogen) result in a substantial increase in potency. Furthermore, we describe how relative binding free energies can be estimated by combining the water displacement energy with complementary terms from an implicit solvent molecular mechanics description binding.
Characterizing antiprion compounds based on their binding properties to prion proteins: Implications as medical chaperones

A variety of antiprion compounds have been reported that are effective in ex vivo and in vivo treatment experiments. However, the molecular mechanisms for most of these compounds remain unknown. Here we classified antiprion mechanisms into four categories: I, specific conformational stabilization; II, nonspecific stabilization; III, aggregation; and IV, interaction with molecules other than PrPC. To characterize antiprion compounds based on this classification, we determined their binding affinities to PrPC using surface plasmon resonance and their binding sites on PrPC using NMR spectroscopy. GN8 and GJP49 bound specifically to the hot spot in PrPC, and acted as “medical chaperones” to stabilize the native conformation. Thus, mechanisms I was predominant. In contrast, quinacrine and epigallocathechin bound to PrPC rather nonspecifically; these may stabilize the PrPC conformation nonspecifically including the interference with the intermolecular interaction following mechanism II. Congo red and pentosan polysulfate bound to PrPC and caused aggregation and precipitation of PrPC, thus reducing the effective concentration of prion protein. Thus, mechanism III was appropriate. Finally, CP‐60, an edarabone derivative, did not bind to PrPC. Thus these were classified into mechanism IV. However, their antiprion activities were not confirmed in the GT + FK system, whose details remain to be elucidated. This proposed antiprion mechanisms of diverse antiprion compounds could help to elucidate their antiprion activities and facilitate effective antiprion drug discovery.
Alveolar-to-Rhotic Coarticulation in North American English: A Preliminary EPG Study

This paper reports results of an electropalatographic (EPG) study of alveolar-torhotic coarticulation in North American English. Data with alveolars /d/ and /n/ occurring in various rhotic contexts were collected from a single female speaker. The results showed a continuum of backing of the primary constriction from alveolar to post-alveolar or retroflex as a function of the absence or presence of one or more rhotic segments in the word and their proximity to the alveolar. These findings are interpreted as coarticulation of alveolars to the more constrained rhotic approximant and rhotacized vowels, and to different degrees of overlap of alveolar and rhotic gestures. The results, albeit preliminary, provide evidence for retroflexed allophones of English alveolars and point to articulatory sources of phonological patterns of retroflexion.
A study of bedside ocular ultrasonography in the emergency department.

UNLABELLED The use of ocular ultrasonography for the evaluation of emergency patients has recently been described in the emergency medicine (EM) literature. There are a number of potential uses that may greatly aid the emergency physician (EP) and avoid lengthy consultation or other diagnostic tests. OBJECTIVE To examine the accuracy of bedside ultrasonography as performed by EPs for the evaluation of ocular pathology. METHODS This prospective, observational study took place in a high-volume, suburban community hospital with an EM residency program. All patients arriving with a history of eye trauma or acute change in vision were eligible to participate in the study. A 10-MHz linear-array transducer was used for imaging. All imaging was performed through a closed eyelid, using water-soluble ultrasound gel. Investigators filled out standardized data sheets and all examinations were taped for review. All ultrasound examinations were followed by orbital computed tomography or complete ophthalmologic evaluation from the ophthalmology service. Statistical analysis included sensitivity, specificity, and positive and negative predictive values. RESULTS Sixty-one patients were enrolled in the study; 26 were found to have intraocular pathology on ultrasound. Of these, three had penetrating globe injuries, nine had retinal detachments, one had central retinal artery occlusion, and two had lens dislocations. The remaining pathology included vitreous hemorrhage and vitreous detachment. Emergency sonologists were in agreement with the criterion standard examination in 60 out of 61 cases. CONCLUSIONS Emergency bedside ultrasound is highly accurate for ruling out and diagnosing ocular pathology in patients presenting to the emergency department. Further, it accurately differentiates between pathology that needs immediate ophthalmologic consultation and that which can be followed up on an outpatient basis.
Attitudes Towards the Use of Mobile Learning: A Case Study from the Gulf Region

In the last few years, the way we learn has been shifted dramatically from traditional classrooms depending on printed papers into E-learning depending on digital pages. Mobile learning (M-learning) is a recent technology that has been developed rapidly to deliver E-learning using personal mobile devices without posing any restrictions on time and location. In this work, we investigate students and faculty members’ attitudes towards the use of M-learning in higher educational institutions within two countries in the Gulf Region (Oman & UAE). Two questionnaire surveys have been conducted: one for students and another for faculty members. In these surveys, 383 students and 54 instructors have taken part within the study. An independent sample t-test was performed to examine whether there exist a significant difference among the students’ attitudes and the faculty members’ attitudes towards the use of M-learning with regard to gender and country. Results indicated that students in the UAE were more positive towards the use of M-learning than those in Oman. Moreover, results revealed that 99% of the students own mobile devices, in particular smartphones and tablets, while only 1% has not. Results of this study could help policy makers for better decision making in building the M-learning infrastructure in the higher educational institutions in general and specifically within the Arab Gulf region.
Treatment of invasive infections due to rare or emerging yeasts and moulds

Emerging fungal infections represent a serious problem in an immunocompromised host. Rapid developments in in vitro antifungal susceptibility testing and the availability of several new antifungal agents have provided excellent opportunities to treat infections that are caused by various Candida spp. and to some extend by Aspergillus spp. However, recently the epidemiology of fungal infections has significantly changed and several new pathogens have emerged. This article attempts to summarise the available data on the management of emerging infections with fungal infections that have recently gained importance. Updated recommendations on antifungal treatment are also discussed.
Diplodia scrobiculata found in the southern hemisphere

Summary Diplodia scrobiculata, a latent pathogen of Pinus spp. and other conifers with a limited distribution in the United States, Mexico and southern Europe, has not been reported previously in the southern hemisphere. This is unlike its close relative Diplodia pinea that is found in most parts of the world where pines are native or have been introduced. During an intensive D. pinea survey conducted in Pinus patula plantations in eastern parts of South Africa, a small number of isolates atypical of D. pinea were found. Morphological studies and DNA sequence comparisons showed that these isolates represent D. scrobiculata. Microsatellite analyses suggest that the South African isolates of D. scrobiculata might have originated from California. Pathogenicity tests showed that some of the D. scrobiculata isolates were as pathogenic as those of D. pinea on Pinus radiata and Pinus elliottii.
Septoplasty with or without postoperative nasal packing? Prospective study

Anterior nasal packing is carried out in a number of nasal surgeries, especially in septoplasty. However, it is not an innocuous procedure and for this its benefit has been challenged. Objective To assess the need for anterior nasal packing and the quality of life of patients submitted to septoplasty. Method Patients submitted to septoplasty with or without inferior turbinoplasty were randomized to receive or not anterior nasal packing postoperatively. We recorded and compared postoperative data (pain and bleeding). Quality of life was assessed before and after surgery. This is a randomized prospective study. Results We had 73 patients (37 packed and 36 who did not receive a nasal packing) with a minimum follow-up of 3 months. Patients with nasal packing complained more of nasal pain and headache in the immediate postoperative period. Of these patients, 75.7% reported moderate/intense pain upon nasal packing removal. Bleeding was more frequent in those patients who did not receive a nasal packing, and only 1 patient required packing. All the patients enjoyed an improvement in quality of life. Conclusion Septoplasty improves the quality of life of patients with septal deviation and nasal obstruction. Routine use of anterior nasal packing should be challenged for not presenting proven benefit.