Miracle-dz/UniFAR-FTUs huggingface dataset:
Italian Validation of the Meaning in Life Questionnaire: Factor Structure, Reliability, Convergent, and Discriminant Validity

Several studies highlighted the role of meaning in life as a major component of well-being and researchers have developed different measures to assess the features of this construct. In the present study, the psychometric properties of the Meaning in Life Questionnaire were investigated in the Italian context. The Meaning in Life Questionnaire is a 10-item scale measuring perceived presence of and search for meaning in life, conceptualized as two separate factors. The former refers to perceived meaning and purpose in life, the latter to the active commitment to find meaning in life. Participants were 464 adults aged 20 to 60 years (M = 39.34; SD = 10.86; 54.7% women). Factor structure was inspected through exploratory and confirmatory factor analyses using a split-sample approach. Internal consistency was assessed through Cronbach’s alphas, interitem and item-scale correlations. Convergent and discriminant validity with measures of well-being, personality, mental health, and physical health were also evaluated. Factor analyses supported the adequacy of the Meaning in Life Questionnaire two-factor structure in the Italian context; internal consistency measures corroborated the measure’s reliability; and correlation matrix coefficients sustained convergent and discriminant validity. Results showed that the Meaning in Life Questionnaire is a valid and reliable measure to assess meaning in life and its relationship with well-being within the Italian context.
Beyond stability constraints: a biophysical model of enzyme evolution with selection for stability and activity

Proteins trace trajectories in sequence space as their amino acids become substituted by other amino acids. The number of substitutions per unit time, the rate of evolution, varies among sites because of biophysical constraints. Several properties that characterize sites’ local environments have been proposed as biophysical determinants of site-specific evolutionary rates. Thus, rate increases with increasing solvent exposure, increasing flexibility, and decreasing local packing density. For enzymes, rate increases also with increasing distance from the protein’s active residues, presumably due to functional constraints. The dependence of rates on solvent accessibility, packing density, and flexibility has been mechanistically explained in terms of selection for stability. However, as I show here, a stability-based model fails to reproduce the observed rate-distance dependence, overestimating rates close to the active residues and underestimating rates of distant sites. Here, I pose a new biophysical model of enzyme evolution with selection for stability and activity (MSA) and compare it with a stability-based counterpart (MS). Testing these models on a structurally and functionally diverse dataset of monomeric enzymes, I found that MSA fits observed rates better than MS for most proteins. While both models reproduce the observed dependence of rates on solvent accessibility, packing, and flexibility, MSA fits these dependencies somewhat better. Importantly, while MS fails to reproduce the dependence of rates on distance from the active residues, MSA accounts for the rate-distance dependence quantitatively. Thus, the variation of evolutionary rate among enzyme sites is mechanistically underpinned by natural selection for both stability and activity.
Pharmacokinetics of an intravenous and oral dose of enrofloxacin in white rhinoceros (Ceratotherium simum)

South Africa currently loses over 1000 white rhinoceros (Ceratotherium simum) each year to poaching incidents, and numbers of severely injured victims found alive have increased dramatically. However, little is known about the antimicrobial treatment of wounds in rhinoceros. This study explores the applicability of enrofloxacin for rhinoceros through the use of pharmacokinetic-pharmacodynamic modelling. The pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin were evaluated in five white rhinoceros after intravenous (i.v.) and after successive i.v. and oral administration of 12.5 mg/kg enrofloxacin. After i.v. administration, the half-life, area under the curve (AUCtot ), clearance and the volume of distribution were 12.41 ± 2.62 hr, 64.5 ± 14.44 μg ml-1 hr-1 , 0.19 ± 0.04 L h-1 kg-1 , and 2.09 ± 0.48 L/kg, respectively. Ciprofloxacin reached 26.42 ± 0.05% of the enrofloxacin plasma concentration. After combined i.v. and oral enrofloxacin administration oral bioavailability was 33.30 ± 38.33%. After i.v. enrofloxacin administration, the efficacy marker AUC24 : MIC exceeded the recommended ratio of 125 against bacteria with an MIC of 0.5 μg/mL. Subsequent intravenous and oral enrofloxacin administration resulted in a low Cmax: MIC ratio of 3.1. The results suggest that intravenous administration of injectable enrofloxacin could be a useful drug with bactericidal properties in rhinoceros. However, the maintenance of the drug plasma concentration at a bactericidal level through additional per os administration of 10% oral solution of enrofloxacin indicated for the use in chickens, turkeys and rabbits does not seem feasible.
Humidity Sensing Behavior of Endohedral Li-Doped and Undoped SWCNT/SDBS Composite Films

We have investigated single-walled carbon nanotube (SWCNT) networks wrapped with the cationic surfactant sodium dodecyl-benzenesulfonate (SBDS) as promising candidates for water detection. This is the first time that the humidity behavior of endohedral Li-doped (Li@) and undoped SWCNTs/SDBS has been shown. We identified a strong and almost monotonic decrease in resistance as humidity increased from 11 to 97%. Sensitivities varied between −3 and 65% in the entire humidity range. Electrical characterization, Raman spectroscopy, and high-resolution transmission electron microscopy (HRTEM) analysis revealed that a combination of the electron donor behavior of the water molecules with Poole-Frenkel conduction accounted for the resistive humidity response in the Li@SWCNT/SDBS and undoped SWCNT/SDBS networks. We found that Li@SWCNTs boosted the semiconducting character in mixtures of metallic/semiconducting SWCNT beams. Moreover, electrical characterization of the sensor suggested that endohedral Li doping produced SWCNT beams with high concentration of semiconducting tubes. We also investigated how frequency influenced film humidity sensing behavior and how this behavior of SWCNT/SDBS films depended on temperature from 20 to 80 °C. The present results will certainly aid design and optimization of SWCNT films with different dopants for humidity or gas sensing in general.
Disk-to-Disk Data Transfer using A Software Defined Networking Solution

There have been efforts towards improving the network performance using software defined networking solutions. One such work is Steroid OpenFlow Service (SOS), which utilizes multiple parallel TCP connections to enhance the network performance transparently to the user. SOS has shown significant improvements in the memory-to-memory data transfer throughput; however, it’s performance for disk-to-disk data transfer hasn’t been studied. For computing applications involving big data, the data files are stored on non-volatile storage devices separate from the computing servers. Before computing can occur, large volumes of data must be fetched from the “remote” storage devices to the computing server’s local storage device. Since hard drives are the most commonly adopted storage devices today, the process is often called “disk-to-disk” data transfer. For production high performance computing facilities, specialized high throughput data transfer software will be provided for users to copy the data first to a data transfer node before copying to the computing server. Disk-to-Disk data transfer’s throughput performance depends on the network throughput between servers and disk access performance between each server and its storage device. Due to large data sizes the storage devices are typically parallel file systems spanning multiple disks. Disk operations in the disk-to-disk data transfer includes disk read and write operations. The read operation in the transfer is to read the data from the disks and store it in memory. The second step in the transfer is to send out the data to the network through the network interface. Data reaching the destination server is then stored to the disk. Data transfer is faced by multiple delays and is limited at each step of the transfer. To date, one commonly adopted data transfer solution is GridFTP developed by the Argonne National Laboratory. It requires custom application installations and configurations on the hosts. SOS, on the other hand, is a transparent network application without special user software. In this thesis, disk-to-disk data transfer performance is studied with both
Isolation stress impacts Met-enkephalin in the hypothalamo-pituitary-adrenocortical axis in growing Polish Mountain sheep: a possible role of the opioids in modulation of HPA axis

Abstract It was hypothesized that there is cross-talk between the classical constituents of the hypothalamo-pituitary-adrenocortical axis (HPA) and Met-enkephalin in the HPA axis. The study examined effects of isolation stress, sex, and age on concentrations of native Met-enkephalin and pro-enkephalin (PENK) gene expression in tissues of the HPA (hypothalamus, pituitary gland and adrenal cortex) in 3-, 6- and 9-month old female and male lambs. In addition, the effects of isolation stress on in vitro release Met-enkephalin from fragments of the hypothalamus or adrenal cortex were examined. Isolation stress was followed by decreases in the concentration of Met-enkephalin in both the pituitary gland and adrenal cortex. There were also increases in the hypothalamic concentration of Met-enkephalin together with increases in PENK gene expression in the HPA in 6- and 9-months old females and males. There were reductions in release of Met-enkephalin from hypothalamic and adrenocortical tissue in vitro after isolation stress. In the presence of naltrexone, there were increases in basal release in vitro of Met-enkephalin from hypothalamic tissue from control and stressed female lambs but a decrease in tissue from stressed male lambs. In a somewhat similar manner, the presence of naltrexone was associated with increases in the basal release of Met-enkephalin from adrenocortical tissue from control female lambs but a decrease with tissue from stressed female and both stressed and control male lambs. Lay summary The present studies examine the impact of isolation stress on Met-enkephalin in growing female and male lambs. The results clearly showed the involvement of Met-enkephalin modulation of the psychological stress response in growing female and male lambs.
Injury to hypothalamic Sim1 neurons is a common feature of obesity by exposure to high‐fat diet in male and female mice

The hypothalamus is essential for regulation of energy homeostasis and metabolism. Feeding hypercaloric, high‐fat (HF) diet induces hypothalamic arcuate nucleus injury and alters metabolism more severely in male than in female mice. The site(s) and extent of hypothalamic injury in male and female mice are not completely understood. In the paraventricular nucleus (PVN) of the hypothalamus, single‐minded family basic helix‐loop helix transcription factor 1 (Sim1) neurons are essential to control energy homeostasis. We tested the hypothesis that exposure to HF diet induces injury to Sim1 neurons in the PVN of male and female mice. Mice expressing membrane‐bound enhanced green fluorescent protein (mEGFP) in Sim1 neurons (Sim1‐Cre:Rosa‐mEGFP mice) were generated to visualize the effects of exposure to HF diet on these neurons. Male and female Sim1‐Cre:Rosa‐mEGFP mice exposed to HF diet had increased weight, hyperleptinemia, and developed hepatosteatosis. In male and female mice exposed to HF diet, expression of mEGFP was reduced by > 40% in Sim1 neurons of the PVN, an effect paralleled by cell apoptosis and neuronal loss, but not by microgliosis. In the arcuate nucleus of the Sim1‐Cre:Rosa‐mEGFP male mice, there was decreased alpha‐melanocyte‐stimulating hormone in proopiomelanocortin neurons projecting to the PVN, with increased cell apoptosis, neuronal loss, and microgliosis. These defects were undetectable in the arcuate nucleus of female mice exposed to the HF diet. Thus, injury to Sim1 neurons of the PVN is a shared feature of exposure to HF diet in mice of both sexes, while injury to proopiomelanocortin neurons in arcuate nucleus is specific to male mice.
Suitability study of current endovascular aortic repair devices based on real-life anatomic data

ABSTRACT Background: There are a number of stent-grafts in the market, designed to handle difficult anatomies during endovascular aortic repair. The aim of this study is to determine which stent-graft is the most appropriate to accommodate the majority of infra-renal aortic aneurysms cases. An additional aim is to determine what stent-graft characteristics influence the suitability of the stent-graft. Research design and methods: Retrospective cohort study on the suitability of available stent-grafts for infrarenal aneurysm (AAA) repair based on real-life anatomic data. The anatomical characteristics of 235 AAA were measured manually using specialized software. All measurements were compared to the official instructions-for-use (IFU) using a purpose-specific server-side script. The proper function of the script was additionally confirmed. Results: The highest suitability rate was achieved by Medtronic Endurant. The majority of stent-grafts failed to meet the respective AAA anatomy by one IFU characteristic. Stent-grafts that failed by only one IFU characteristic by ≥80% of unsuitable cases tend to have higher suitability rates. Neck dimensions and CIA diameter are the two characteristics significantly influencing the suitability rate. Conclusions: Any stent-graft with high suitability rates should be available off-the-shelf in any vascular department in order to cover the majority of cases. For challenging cases, special advantages of the stent-grafts should be taken under consideration.
“Stay balanced” – effectiveness of evidence-based balance training for older adults transferred into a physical therapy primary care setting – a pilot study

Abstract Purpose: To evaluate the effects of the Stay Balanced program when this is transferred into a clinical setting regarding balance, gait speed, leg muscle strength, concerns about falling, and physical activity. Method: Implementation pilot study with a pre-post intervention design. Fifteen older adults, 75–91 years of age, participated in a progressive balance training program with a focus on divided attention. The balance training was performed in group sessions twice a week for 10 weeks at a primary care physical therapy clinic. Training efficacy was evaluated after completion of training as well as after 3 months using the Mini-Balance Evaluation Systems Test (Mini-BESTest), 10-meter walk test, 30-s chair stand test, Fall Efficacy Scale-International (FES-I), and steps/day. Results: Significant improvements were shown at the 10-week follow up for balance, gait speed, leg muscle strength, and concerns about falling (p < 0.008). At the 3-month follow-up balance, leg muscle strength and concerns about falling showed persistent improvement compared to baseline (p < 0.045). No significant differences were found for physical activity. Conclusions: This study confirms the results of our previous randomized controlled trials (RCTs), and suggests that the Stay Balanced program can be transferred to clinical physiotherapy practice. The program was appreciated by the participants and proved to be safe, effective, and feasible in primary care. Implication for rehabilitation The Stay Balance program can easily be transferred to clinical practice without losing the effectiveness of the intervention in older adults with balance problems. The program was appreciated by the participants and proved to be safe, effective, and feasible when executed in primary care. Stay Balance program is an individually adjusted and progressive group balance training including exercises with divided attention that can easily be transferred to tasks in daily life.
Changes in high endothelial venules in lymph nodes after vascularized and nonvascularized lymph node transfer in a murine autograft model

Vascularized lymph node transfer (LNT) is gaining popularity in the treatment of lymphedema. However, it is unclear whether the vascularization of transferred lymph nodes (LNs) contributes to functional improvement. High endothelial venules (HEVs) are specialized vessels that allow lymphocytes to enter LNs. In this study, we compared the numbers of HEVs and lymphocytes in LNs after vascularized and nonvascularized LNT.
Acceptance and Commitment Therapy for Parental Management of Childhood Asthma: An RCT

Through an RCT, we show that ACT plus asthma education for parents improves their psychological well-being and children’s asthma outcomes. BACKGROUND: Few trials have been conducted to address the psychological difficulties of parents in managing their child’s asthma. Fostering parental psychological flexibility through Acceptance and Commitment Therapy (ACT) may help parents to accept these psychological difficulties and improve their management of childhood asthma. METHODS: In this randomized controlled trial, a 4-session, group-based ACT plus asthma education (ACT group) was compared with an asthma education talk plus 3 telephone follow-ups (control group) to train parents of children diagnosed with asthma. The use of health care services due to asthma exacerbations in children and the psychological well-being of their parents were assessed before, immediately after, and at 6 months after the intervention. RESULTS: A total of 168 parents and their children aged 3 to 12 years with asthma were consecutively recruited in a public hospital in Hong Kong. When compared with the control group, children whose parents were in the ACT group made significantly fewer emergency department visits (adjusted 6-month incidence rate ratio = 0.20; confidence interval [CI] 0.08 to 0.53; P = .001) due to asthma exacerbations at 6 months postintervention. These parents also reported a decrease in psychological inflexibility (mean difference = −5.45; CI −7.71 to −3.30; P = .014), less anxiety (mean difference = −2.20; CI −3.66 to −0.73; P = .003), and stress (mean difference = −2.50; CI −4.54 to −0.47; P = .016). CONCLUSIONS: Integrating ACT into parental asthma education was effective at decreasing parental anxiety and stress and reducing the asthma-related emergency department visits of children at 6 months postintervention.
Estimating the Fitness Effect of Deleterious Mutations During the Two Phases of the Life Cycle: A New Method Applied to the Root-Rot Fungus Heterobasidion parviporum

Many eukaryote species, including taxa such as fungi or algae, have a lifecycle with substantial haploid and diploid phases. A recent theoretical model predicts that such haploid-diploid lifecycles are stable over long evolutionary time scales when segregating deleterious mutations have stronger effects in homozygous diploids than in haploids and when they are partially recessive in heterozygous diploids. The model predicts that effective dominance—a measure that accounts for these two effects—should be close to 0.5 in these species. It also predicts that diploids should have higher fitness than haploids on average. However, an appropriate statistical framework to conjointly investigate these predictions is currently lacking. In this study, we derive a new quantitative genetic model to test these predictions using fitness data of two haploid parents and their diploid offspring, and genome-wide genetic distance between haploid parents. We apply this model to the root-rot basidiomycete fungus Heterobasidion parviporum—a species where the heterokaryotic (equivalent to the diploid) phase is longer than the homokaryotic (haploid) phase. We measured two fitness-related traits (mycelium growth rate and the ability to degrade wood) in both homokaryons and heterokaryons, and we used whole-genome sequencing to estimate nuclear genetic distance between parents. Possibly due to a lack of power, we did not find that deleterious mutations were recessive or more deleterious when expressed during the heterokaryotic phase. Using this model to compare effective dominance among haploid-diploid species where the relative importance of the two phases varies should help better understand the evolution of haploid-diploid life cycles.
Molecular evidence of field cancerization initiated by diabetes in colon cancer patients

The potential involvement of type 2 diabetes mellitus (T2DM) as a risk factor for colon cancer (CC) has been previously reported. While several clinical studies show a higher incidence of CC and a lower survival rate in diabetics, others report no association. Our own experience indicates that diabetes does not seem to worsen the prognosis once the tumor is present. Despite this controversy, there are no wide‐spectrum molecular studies that delve into the impact of T2DM‐related mechanisms in colon carcinogenesis. Here, we present a transcriptomic and proteomic profiling of paired tumor and normal colon mucosa samples in a cohort of 42 CC patients, 23 of which have T2DM. We used gene set enrichment and network approaches to extract relevant pathways in diabetics, referenced them to current knowledge, and tested them using in vitro techniques. Through our transcriptomics approach, we identified an unexpected overlap of pathways overrepresented in diabetics compared to nondiabetics, in both tumor and normal mucosa, including diabetes‐related metabolic and signaling processes. Proteomic approaches highlighted several cancer‐related signaling routes in diabetics found only in normal mucosa, not in tumors. An integration of the transcriptome and proteome analyses suggested the deregulation of key pathways related to colon carcinogenesis which converged on tumor initiation axis TEAD/YAP‐TAZ as a potential initiator of the process. In vitro studies confirmed upregulation of this pathway in nontumor colon cells under high‐glucose conditions. In conclusion, T2DM associates with deregulation of cancer‐related processes in normal colon mucosa adjacent to tissue which has undergone a malignant transformation. These data support that in diabetic patients, the local microenvironment in normal colon mucosa may be a factor driving field cancerization promoting carcinogenesis. Our results set a new framework to study links between diabetes and colon cancer, including a new role of the TEAD/YAP‐TAZ complex as a potential driver.