Miracle-dz/UniFAR-FTUs huggingface dataset:
MDCT has the potential to predict percutaneous coronary intervention outcome in swine model: microscopic validation

Background Volumes and sizes of dislodged coronary microemboli vary during PCI so their effects at the left ventricular (LV) and cellular levels cannot be quantified. Furthermore, biopsy for tissue characterization is not an option in PCI patients. Purpose To characterize and validate microinfarct size, LAD territory where microinfarct were found using multidetector computed tomography (MDCT), histochemical staining and microscopy as a function of microemboli volumes and to scale the effects of microemboli volumes on LV function. Material and Methods Under X-ray guidance, a 3F catheter was inserted into LAD coronary artery of 14 pigs for delivering 16 mm3 or 32 mm3 of 40-120 μm microemboli. MDCT imaging/histochemical staining/ microscopy were performed 3 days later and used to characterize regional and global structural and functional changes in LV by threshold/planimetric methods. Results MDCT and ex-vivo methods were able to quantify microinfarct size and LAD territory where microinfarct was found as a function of volumes. However, MDCT and histochemical staining significantly underestimated microinfarct size and territory where microinfarct was found compared with microscopy. MDCT demonstrated the functional changes and showed a moderate correlation between LV ejection fraction and microinfarct size (r = 0.53). Microscopy provided higher spatial resolution for measuring islands of necrotic cells, which explains the difference in measuring structural changes. Conclusion MDCT showed the difference in microinfarct size and LAD territory as a function of microemboli volumes and scaled the changes in LV function. This experimental study gives clinicians a reference for the effects of defined microemboli volumes on myocardial viability and LV function and the under-estimation of microinfarct on MDCT.
Xylitol Absorption in Healthy Men

Xylitol absorption from the human intestinal tract was studied directly, by aspiration and analysis of ileul contents after ingestion of xylitol (5 to 30 gm.) mixed with glucose anda nonabsorbable marker, polyethylene glycol. Studies in five healthy subjects showed that xylitolabsorption ranged from 49 to 95 per cent. Chronic xylitol administration (30 gm, daily for two tothree weeks) did not alter significantly the rate of xylitol absorption. Neither symptoms nor laboratory abnormalities were observed following chronic xylitol ingestion. Nearly complete intestinal absorption must be taken into account in considering the usefulness of xylitol in the diet, since untoward responses to intravenous xylitol infusions have been reported.
Pediatric blunt abdominal injury: age is irrelevant and delayed operation is not detrimental.

BACKGROUND During the past 40 years, management of solid organ injury in pediatric trauma patients has shifted to highly successful nonoperative management. Our purpose was to characterize children requiring operative intervention. We hypothesized that older children would be more likely to require operative intervention. In particular, we wanted to examine potential outcome disparities between children who were operated upon immediately and those in whom attempted nonoperative management failed. Additionally, we asked whether attempted nonoperative management, when failed, put children at higher risk for mortality or morbidities such as increased blood product transfusions or lengths of stays. METHODS Retrospective cohorts from seven Level I pediatric trauma centers were identified. Blunt splenic, hepatic, renal, or pancreatic injuries were documented in 2,944 children <1 to 19 years of age from January 1993 to December 2002. Data collected included demographics, hemodynamics, blood transfusions, Glasgow Coma Scale score, Injury Severity Score, hospital length of stay (LOS), intensive care unit (ICU) LOS, and mortality. Analysis involved 140 (4.8%) of 2,944 patients requiring operation. Two cohorts were characterized: (1) immediate operation (IO), defined as laparotomy </=3 hours after arrival (n = 81; 58%) and (2) failed nonoperative management (F-NOM), defined as laparotomy >3 hours after arrival (n = 59; 42%). RESULTS Comparing the two cohorts, no age differences were found. Compared with F-NOM, IO had significantly worse hemodynamics, Injury Severity Score, and Glasgow Coma Scale score and was associated with liver injuries. Pancreatic injuries were significantly associated with F-NOM. While controlling for injury severity to compare IO versus F-NOM, linear regression revealed equivalent blood transfusions, ICU LOS, hospital LOS, and mortality rates. CONCLUSION IO and F-NOM are rare events and independent of age. When operated upon for appropriate physiology, the timing of operation in pediatric solid organ injury is irrelevant and not detrimental with respect to blood transfusion, mortality, ICU and hospital LOS, and resource utilization.
Computer-assisted Reconstruction and Assessment of E. J. Lowe's Modal Ontological Argument

Computers may help us to understand –not just verify– philosophical arguments. By utilizing modern proof assistants in an iterative interpretive process, we can reconstruct and assess an argument by fully formal means. Through the mechanization of a variant of St. Anselm’s ontological argument by E. J. Lowe, which is a paradigmatic example of a natural-language argument with strong ties to metaphysics and religion, we offer an ideal showcase for our computer-assisted interpretive method.
Revisiting the historical distribution of Seasonally Dry Tropical Forests: new insights based on palaeodistribution modelling and palynological evidencegeb

Aim To investigate the potential distribution of Seasonally Dry Tropical Forests (SDTFs) during the Quaternary climatic fluctuations; to reassess the formerly proposed ‘Pleistocenic arc hypothesis’ (PAH); and to identify historically stable and unstable areas of SDTF distributions in the light of palaeodistribution modelling. Location SDTFs in lowland cis-Andean eastern-central South America. Methods We first developed georeferenced maps depicting the current distributional extent of SDTFs under two distinct definitions (narrow and broad). We then generated occurrence datasets, which were used with current and past bioclimatic variables to predict SDTF occurrence by implementing the maximum entropy machine-learning algorithm. We obtained historical stability maps by overlapping the presence/absence projections of each of three climatic scenarios [current, 6 kyr bp during the Holocene, and 21 kyr bp during the Last Glacial Maximum (LGM)]. Finally, we checked the consistencies of the model prediction with qualitative comparisons of vegetation types inferred from available fossil pollen records. Results The present-day SDTF distribution is disjunct, but we provide evidence that it was even more disjunct during the LGM. Reconstructions support a progressive southward and eastward expansion of SDTFs on a continental scale since the LGM. No significant expansion of SDTFs into the Amazon Basin was detected. Areas of presumed long-term stability are identified and confirmed (the three nuclear regions, Caatinga, Misiones and Piedmont, plus the Chiquitano region), and these possibly acted as current and historical refugial areas. Main conclusions The LGM climate was probably too dry and cold to support large tracts of SDTF, which were restricted to climatically favourable areas relative to the present day (in contrast with the PAH, as it was originally conceived). Expansions of SDTFs are proposed to have occupied the southern portion of Caatinga nucleus more recently during the early–middle Holocene transition. We propose an alternative scenario amenable to further testing of an earlier SDTF expansion (either at the Lower Pleistocene or the Tertiary), followed by fragmentation in the LGM and secondary expansion in the Holocene. The stability maps were used to generate specific genetic predictions at both continental and regional scales (stable areas are expected to have higher genetic diversity and endemism levels than adjacent unstable areas) that can be used to direct field sampling to cover both stable (predicted refugia) and unstable (recently colonized) areas. Lastly, we discuss the possibility that SDTFs may experience future expansion under changing climate scenarios and that both stable and unstable areas should be prioritized by conservation initiatives.
Necessity of an adequate nerve supply for regeneration of the amputated pectoral fin in the teleost Fundulus.

The present work deals with determination of the threshold of nerve fibers per unit of amputation surface necessary for regeneration of the pectoral fins of a teleost, Fundulus. Partial denervation of the amputated pectoral fins, i.e., resection of one or two of the three nerves of the brachial (=pectoral) plexus revealed that the presence of a single one allows the amputated fin to regenerate. From these data and others obtained previously, it is concluded that the nervous requirements for a teleost fin to regenerate are similar or slightly lower than those for tetrapods, for example in the newt, which are capable of appendage regeneration.
The Temporospatial and Kinematic Characteristics of Typical Tai Chi Movements: Repulse Monkey and Wave-hand in Cloud

Tai Chi movements share many similar biomechanical features with normal walking. The purpose of the study was to examine and to compare the temporospatial and kinematic features of two typical Tai Chi movements with forward walking. Experienced male Tai Chi practitioners (n = 15) between the ages of 65 to 75, performed ‘Repulse Monkey (RM)’, ‘Wave-hand in Cloud (WHIC)’, and forward walking. The results showed that stride width was larger for WHIC compared with walking; double-support time was longer for RM while both Tai Chi movements spent less time in single-support. The Tai Chi movements elicit larger vertical and mediolateral displacement of the centre of mass compared with walking. During RM and WHIC, the knees remained flexed while RM had notably larger abduction and adduction of the knee joint. The gentle and fluid Tai Chi movements have unique biomechanical features that may result in special training to postural control capacity.
From Many Hosts, One Accidental Pathogen: The Diverse Protozoan Hosts of Legionella

The 1976 outbreak of Legionnaires' disease led to the discovery of the intracellular bacterial pathogen Legionella pneumophila. Given their impact on human health, Legionella species and the mechanisms responsible for their replication within host cells are often studied in alveolar macrophages, the primary human cell type associated with disease. Despite the potential severity of individual cases of disease, Legionella are not spread from person-to-person. Thus, from the pathogen's perspective, interactions with human cells are accidents of time and space—evolutionary dead ends with no impact on Legionella's long-term survival or pathogenic trajectory. To understand Legionella as a pathogen is to understand its interaction with its natural hosts: the polyphyletic protozoa, a group of unicellular eukaryotes with a staggering amount of evolutionary diversity. While much remains to be understood about these enigmatic hosts, we summarize the current state of knowledge concerning Legionella's natural host range, the diversity of Legionella-protozoa interactions, the factors influencing these interactions, the importance of avoiding the generalization of protozoan-bacterial interactions based on a limited number of model hosts and the central role of protozoa to the biology, evolution, and persistence of Legionella in the environment.
Ablation of the transcription factors E2F1-2 limits neuroinflammation and associated neurological deficits after contusive spinal cord injury

Traumatic spinal cord injury (SCI) induces cell cycle activation (CCA) that contributes to secondary injury and related functional impairments such as motor deficits and hyperpathia. E2F1 and E2F2 are members of the activator sub-family of E2F transcription factors that play an important role in proliferating cells and in cell cycle-related neuronal death, but no comprehensive study have been performed in SCI to determine the relative importance of these factors. Here we examined the temporal distribution and cell-type specificity of E2F1 and E2F2 expression following mouse SCI, as well as the effects of genetic deletion of E2F1-2 on neuronal cell death, neuroinflammation and associated neurological dysfunction. SCI significantly increased E2F1 and E2F2 expression in active caspase-3+ neurons/oligodendrocytes as well as in activated microglia/astrocytes. Injury-induced up-regulation of cell cycle-related genes and protein was significantly reduced by intrathecal injection of high specificity E2F decoy oligodeoxynucleotides against the E2F-binding site or in E2F1-2 null mice. Combined E2F1+2 siRNA treatment show greater neuroprotection in vivo than E2F1 or E2F2 single siRNA treatment. Knockout of both E2F1 and E2F2 genes (E2Fdko) significantly reduced neuronal death, neuroinflammation, and tissue damage, as well as limiting motor dysfunction and hyperpathia after SCI. Both CCA reduction and functional improvement in E2Fdko mice were greater than those in E2F2ko model. These studies demonstrate that SCI-induced activation of E2F1-2 mediates CCA, contributing to gliopathy and neuronal/tissue loss associated with motor impairments and post-traumatic hyperesthesia. Thus, E2F1-2 provide a therapeutic target for decreasing secondary tissue damage and promoting recovery of function after SCI.
Genetic analysis of herd life in Canadian dairy cattle on a lactation basis using the Survival Kit

Longevity is a highly desirable trait that considerably affects overall profitability. With increased longevity the mean production of the herd increases because a greater proportion of the culling decisions are based on production and the proportion of mature cows, which produce more milk than young, cows is increased. An increased longevity enables higher selection intensity of females and thus greater selection response.
Impact of Geriatric Conditions Versus Medical Diagnoses on ADL Disability Among Nonagenarians and Centenarians

Objective: The authors investigated the relationship of activities of daily living (ADL) disability with medical diseases and geriatric conditions among nonagenarians and centenarians. Method: Cross-sectional, n = 870 Chinese (age range: 90-108 years). Self-reported medical diseases and geriatric-specific conditions were obtained by face-to-face interviews. Biomedical measurements included systolic/diastolic blood pressure, BMI (body mass index), albumin, fasting glucose, creatinine clearance, hemoglobin, and lipid panel. Results: In bivariate analyses, 4 of 7 geriatric conditions (hearing problems, falls, cognitive impairment, fracture), and 6 of 11 biomedical measurements (fasting glucose, cholesterol, LDL, creatinine clearance, hemoglobin, albumin), but none of 10 medical diseases and no degree of comorbidities, were associated with ADL disability. In four different multivariate logistic regression models, two geriatric conditions (impaired cognition, fracture) were significant in three models, respiratory disease in one model, and fasting glucose in two models. Discussion: This study emphasizes the importance of geriatric conditions and their association with ADL disability among the oldest-old adult population.
Efficacy and Safety of Long-Term Sirolimus Therapy for Asian Patients with Lymphangioleiomyomatosis.

RATIONALE Sirolimus has been shown in a randomized, controlled clinical trial to stabilize lung function in patients with lymphangioleiomyomatosis (LAM) treated for a 12-month time period; however the pretreatment decline in lung function after the drug was discontinued indicated that continued exposure is required to suppress disease progression. OBJECTIVES To elucidate the durability and tolerability of long-term sirolimus treatment in Asian patients with LAM. METHODS We conducted a single-arm, open-label, investigator-initiated safety and efficacy study of sirolimus in 63 women with LAM at 9 sites in Japan. Subjects received sirolimus for 2 years at doses adjusted to maintain a trough blood level of 5-15 ng/ml. MEASUREMENTS AND MAIN RESULTS Fifty-two subjects (82.5%) completed the trial with mean drug compliance of more than 80% overall during the study. The number of adverse events was greatest during the initial 6 months of therapy, but they continued to occur with declining frequency throughout the 2-year study period. Of the 1,549 adverse events, 27 were classified as serious, including reversible sirolimus pneumonitis in 3 patients. New hypercholesterolemia occurred in 30 patients (48%); microcytosis in 10 patients; loss of body weight in 33 patients; and increase in blood pressure that required treatment in 5 patients. FEV1, FVC, and quality-of-life parameters were stable in the overall study cohort during the study period, but baseline to 2-year improvements in lung function occurred in the subset of patients with a prior history of chylothorax. CONCLUSIONS Although long-term sirolimus treatment of Asian patients with LAM was associated with a large number of adverse events, including three episodes of pneumonitis, most patients completed the 2-year course of medication with good drug compliance and stable quality of life and lung function.
Excretion of β-Lactam Antibiotics in Sweat—a Neglected Mechanism for Development of Antibiotic Resistance?

ABSTRACT The concentrations of β-lactam antibiotics after standard doses were measured in blood and apocrine (axilla) and eccrine (forearm) sweat from six adult healthy persons. All persons had ceftazidime (axilla, 28.4 μg/ml; forearm, 11 μg/ml) and ceftriaxone (axilla, 8.9 μg/ml; forearm, 2.5 μg/ml) in sweat, and one person had cefuroxime in sweat (axilla, 7.8 μg/ml) (all data are mean peaks). Three persons had benzylpenicillin (axilla, 2.6 to 0.1 μg/ml) and one had phenoxymethylpenicillin (axilla, 0.4 μg/ml) in sweat. Excretion of β-lactam antibiotics in the sweat may explain why staphylococci so rapidly become resistant to these drugs.
A combined electromagnetic and microwave antenna system simulator for radio astronomy

A generic numerical tool has been developed to compute/optimize the sensitivity of array antenna receiver systems. This antenna system simulator is comprised of an ElectroMagnetic (EM) simulator and a MicroWave simulator (MW) for modeling the signal and noise characteristics of each receiver component. This analysis includes accurate modeling of the background noise (sky+ground), which is essential in radio astronomical applications, as well as the losses, reflections and noise contributions that occur in the microwave beamforming network. Validation has been carried out for a 4x1 monopole array receiver system. The modeled absolute power levels for a noisy 'hot' and 'cold' environment are in very good agreement with the measured noise power levels.
Magnetic resonance imaging striatal volumes: A biomarker for clinical trials in Huntington's disease

An abundance of research shows that magnetic resonance imaging (MRI) striatal volumes decrease long before diagnosis of Huntington's disease (HD) and closely track disease progression. Additional research indicates that these volumetric measures meet important criteria for a biomarker that can be used in clinical trials: They are 1) objectively measureable; 2) able to predict known endpoints; and 3) associated with known mechanisms of pathology of the disease. Researchers should consider formal application to regulatory agencies for biomarker status of volumetric MRI striatal measures, because these measures are anticipated to contribute significantly in the assessment of treatment effectiveness in HD. © 2014 International Parkinson and Movement Disorder Society
NUTRITIONAL ASSESSMENT OF PERIPHERAL BLOOD STEM CELL TRANSPLANTATION IN CHILDREN

Peripheral blood stem cell transplantation (PBSCT) has many advantages for patients because hematopoiesis and general condition return to normal more rapidly than they do following bone marrow transplantation. Thus, the authors hypothesize that the nutritional condition of patients also returns to normal more rapidly after PBSCT. The duration of insufficient nutrition was investigated in children undergoing PBSCT. The subjects of this study were 8 patients with malignant diseases. The factors measured were body weight, body fat, cholesterol, albumin, pre-albumin, and retinol-binding protein. These parameters were measured a day before transplantation, and then once a week for 4 weeks after transplantation. All parameters were recovered until day 28 from the lowest level in transplantation. In this study, all parameters returned to normal comparatively early. PBSCT causes little damage to patients' nutrition.
Adenosine 2A receptor promotes collagen production by human fibroblasts via pathways involving cyclic AMP and AKT but independent of Smad2/3

Activation of adenosine A2A receptor (A2AR) promotes fibrosis and collagen synthesis. However, the underlying mechanism is still unclear, not least because cAMP, its principal effector, has been found to inhibit TGFβ1‐induced collagen synthesis. Here, we show that in primary normal human dermal fibroblasts, A2AR stimulation with CGS21680 elicits a modest cAMP increase (150±12% of control; EC50 54.8 nM), which stimulates collagen1 (Col1) and collagen3 (Col3), but maximal cAMP resulting from direct activation of adenylyl cyclase by forskolin (15,689±7038% of control; EC50 360.7 nM) inhibits Col1 and increases Col3. Similar to Col1 expression, fibroblast proliferation increased following physiological cAMP increases by CGS21680 but was inhibited by cAMP increases beyond the physiological range by forskolin. The A2AR‐mediated increase of Col1 and Col3 was mediated by AKT, while Col3, but not Col1, expression was dependent on p38 and repressed by ERK. TGFβ1 induced phosphorylation of Smad2/3 and increased Col3 expression, which was prevented by Smad3 depletion. In contrast, CGS21680 did not activate Smad2/3, and Smad2/3 knockdown did not prevent CGS21680‐induced Col1 or Col3 increases. Our results indicate that cAMP is a concentration‐dependent switch for collagen production via noncanonical, AKT‐dependent, Smad2/3‐independent signaling. These observations explain the paradoxical effects of cAMP on collagen expression. —Perez‐Aso, M. , Fernandez, P., Mediero, A. , Chan, E. S. , and Cronstein, B. N. Adenosine 2A receptor promotes collagen production by human fibroblasts via pathways involving cyclic AMP and AKT but independent of Smad2/3. FASEB J. 28, 802–812 (2014). www.fasebj. org