Miracle-dz/UniFAR-FTUs huggingface dataset:
Type 1 IFN Mediates Cross-Talk between Innate and Adaptive Immunity That Abrogates Transplantation Tolerance12

TLR activation of innate immunity prevents the induction of transplantation tolerance and shortens skin allograft survival in mice treated with costimulation blockade. The mechanism by which TLR signaling mediates this effect has not been clear. We now report that administration of the TLR agonists LPS (TLR4) or polyinosinic:polycytidylic acid (TLR3) to mice treated with costimulation blockade prevents alloreactive CD8+ T cell deletion, primes alloreactive CTLs, and shortens allograft survival. The TLR4- and MyD88-dependent pathways are required for LPS to shorten allograft survival, whereas polyinosinic:polycytidylic acid mediates its effects through a TLR3-independent pathway. These effects are all mediated by signaling through the type 1 IFN (IFN-αβ) receptor. Administration of IFN-β recapitulates the detrimental effects of TLR agonists on transplantation tolerance. We conclude that the type 1 IFN generated as part of an innate immune response to TLR activation can in turn activate adaptive immune responses that abrogate transplantation tolerance. Blocking of type 1 IFN-dependent pathways in patients may improve allograft survival in the presence of exogenous TLR ligands.
Understanding Nonresponse Rates: Insights from 600,000 Opinion Surveys

Despite the central role of surveys in empirical research, academics have not paid enough attention to the factors that affect response rates. This is especially concerning since survey response rates—of both household and opinion surveys—have been declining over time (Meyer, Mok, and Sullivan, 2015), which might lead to distorted survey results. This paper explores how the underlying design of each survey—such as its length and the day of the week in which the survey was conducted—affects the response rates of the survey.
The Small GTPase ROP6 Interacts with NFR5 and Is Involved in Nodule Formation in Lotus japonicus1[C][W][OA]

Nod Factor Receptor5 (NFR5) is an atypical receptor-like kinase, having no activation loop in the protein kinase domain. It forms a heterodimer with NFR1 and is required for the early plant responses to Rhizobium infection. A Rho-like small GTPase from Lotus japonicus was identified as an NFR5-interacting protein. The amino acid sequence of this Rho-like GTPase is closest to the Arabidopsis (Arabidopsis thaliana) ROP6 and Medicago truncatula ROP6 and was designated as LjROP6. The interaction between Rop6 and NFR5 occurred both in vitro and in planta. No interaction between Rop6 and NFR1 was observed. Green fluorescent protein-tagged ROP6 was localized at the plasma membrane and cytoplasm. The interaction between ROP6 and NFR5 appeared to take place at the plasma membrane. The expression of the ROP6 gene could be detected in vascular tissues of Lotus roots. After inoculation with Mesorhizobium loti, elevated levels of ROP6 expression were found in the root hairs, root tips, vascular bundles of roots, nodule primordia, and young nodules. In transgenic hairy roots expressing ROP6 RNA interference constructs, Rhizobium entry into the root hairs did not appear to be affected, but infection thread growth through the root cortex were severely inhibited, resulting in the development of fewer nodules per plant. These data demonstrate a role of ROP6 as a positive regulator of infection thread formation and nodulation in L. japonicus.
Weighted Least‐Squares Approach for Comparing Correlated Kappa

Summary. In the medical sciences, studies are often designed to assess the agreement between different raters or different instruments. The kappa coefficient is a popular index of agreement for binary and categorical ratings. Here we focus on testing for the equality of two dependent kappa coefficients. We use the weighted least‐squares (WLS) approach of Koch et al. (1977, Biometrics33, 133–158) to take into account the correlation between the estimated kappa statistics. We demonstrate how the SAS PROC CATMOD can be used to test for the equality of dependent Cohen's kappa coefficients and dependent intraclass kappa coefficients with nominal categorical ratings. We also test for the equality of dependent Cohen's kappa and dependent weighted kappa with ordinal ratings. The major advantage of the WLS approach is that it allows the data analyst a way of testing dependent kappa with popular SAS software. The WLS approach can handle any number of categories. Analyses of three biomedical studies are used for illustration.
Na(+)-H+ exchanger isoform 1 phosphorylation in normal Wistar-Kyoto and spontaneously hypertensive rats.

Increased activity of the cellular Na(+)-H+ exchanger (NHE) has been documented in various cell types in essential hypertension and in vascular myocytes of the spontaneously hypertensive rat (SHR). The mechanism underlying this abnormality is unclear. Because the NHE can be activated by phosphorylation, we examined phosphorylation of the Na(+)-H+ exchanger isoform 1 (NHE-1) as one possible mechanism for its increased turnover number in cultured vascular myocytes of the SHR. A polyclonal rabbit antibody against a fusion protein consisting of beta-galactosidase and the C-terminus of NHE-1 was used to immunoprecipitate 32P-labeled NHE-1 from cell extracts of SHR and Wistar-Kyoto (WKY) rat vascular myocytes in the absence and presence of 10% fetal calf serum. Immunoprecipitates were separated by SDS-PAGE, and 32P-labeled NHE-1 was quantified from autoradiographs. Similar amounts of NHE-1 protein were detected on Western blots of the cultured vascular myocytes from SHR and WKY rats. In quiescent cells, NHE-1 was significantly more phosphorylated in SHR myocytes than in WKY myocytes (2.17 +/- 0.06-fold enhancement [mean +/- SEM]; P < .001, n = 8). The addition of fetal calf serum to quiescent cells had no significant effect on the phosphorylation of NHE-1 in SHR myocytes. However, NHE-1 phosphorylation fell transiently in serum-treated WKY myocytes, with recovery to control levels after 20 minutes. Measurement of NHE activity using fluorometry confirmed elevated activity in the quiescent SHR myocytes compared with WKY myocytes. Fetal calf serum led to further enhancement of NHE activity in both cell types. These findings suggest that the increased NHE activity in quiescent SHR myocytes may be correlated with enhanced NHE-1 phosphorylation and that serum stimulates NHE activity in both cell types without a further increase in total NHE-1 phosphorylation, indicating a role for non-phosphorylation-dependent regulatory mechanisms.
Adaptation of Listeria monocytogenes in a simulated cheese medium: effects on virulence using the Galleria mellonella infection model

The aim of this study was to evaluate the effect of the acid and salt adaptation in a cheese‐based medium on the virulence potential of Listeria monocytogenes strains isolated from cheese and dairy processing environment using the Galleria mellonella model. Four L. monocytogenes strains were exposed to a cheese‐based medium in conditions of induction of an acid tolerance response and osmotolerance response (pH 5·5 and 3·5% w/v NaCl) and injected in G. mellonella insects. The survival of insects and the L. monocytogenes growth kinetics in insects were evaluated. The gene expression of hly, actA and inlA genes was determined by real‐time PCR. The adapted cells of two dairy strains showed reduced insect mortality (P < 0·05) in comparison with nonadapted cells. Listeria monocytogenes Scott A was the least virulent, whereas the cheese isolate C882 caused the highest insect mortality, and no differences (P > 0·05) was found between adapted and nonadapted cells. The gene expression results evidenced an overexpression of virulence genes in cheese‐based medium, but not in simulated insect‐induced conditions. Our results suggest that adaptation to low pH and salt in a cheese‐based medium can affect the virulence of L. monocytogenes, but this effect is strain dependent.
N 2 teeth in the line of mandibular fractures : epidemiology , management and complications

I. Senior Resident, Division of Oral and Maxillofacial Surgery, Curitiba Dental School, State University of Paraná, Curitiba, Paraná, Brazil. II. Professor, Division of Oral and Maxillofacial Surgery, Curitiba Dental School, State University of Paraná, Curitiba, Paraná, Brazil. III. Professor, Division of Oral and Maxillofacial Surgery, Piracicaba Dental School and Division of Plastic Surgery, School of Medical Sciences, State University of Campinas Unicamp, São Paulo, Brazil. IV. Resident of Oral and Maxillofacial Surgery, Federal University of Paraná, Curitiba, PR, Brazil. V. Professor of the Resident of Oral and Maxillofacial Surgery, Federal University of Paraná, Curitiba, PR, Brazil. ABstrAct Purpose: To examine the incidence, the regions most affected, the management as well as the complications found in teeth involved in the line of mandibular fractures. Methods: Data were collected from patients records treated of mandibular fractures. It was included information such as demographic data, fracture location, presence of teeth in the line of fracture, treatment and complications. Results: The sample has presented a total of 310 lines of fracture, from these, 144 had teeth involved. The most affected area was the angle, followed by the parasymphysis, body, alveolar process and symphysis. There were 196 “fracture line” teeth, 60 of which had been removed. Postoperative complications occurred in 8.6% of the sample. From the cases that presented complications, patients without teeth involved in the fracture line ranged 43.75% and patients with teeth involved in the fracture line reached 56.25%. When the tooth was removed 8.47% of the patients presented complications and when the tooth was retained 2.85%. Conclusion: This study demonstrated that the presence of teeth in the line of fracture is not a limiting factor for the treatment. The complications are more frequently related to the chosen method of treatment than to the involvement of teeth in the line of fracture. Key-Words: mandibular fracture, jaw fracture, complications, tooth nonvitality.
Behaviour problems in pre-school children.

SUMMARY This paper Describes the prevalence and types of behaviour problems found in a sample of 418 pre-school children in North London. The data presented are based on information obtained from the parents of the children concerned. An association between behaviour problems and speech and language delay was found in the children of 2 Years and over. The implications for services to meet the needs of families with pre-school children are discussed.
Maternal separation and early stress cause long‐lasting effects on dopaminergic and endocannabinergic systems and alters dendritic morphology in the nucleus accumbens and frontal cortex in rats

A considerable amount experimental studies have shown that maternal separation (MS) is associated with adult offspring abnormal behavior and cognition disorder. Accordingly, this experimental procedure has been proposed as a predictor for alcohol and drug dependence based on the neurodevelopmental soon after birth. Endocannabinoid system (eCBs) has been implicated in reward processes, including drug abuse and dependence. MS and associated stress causes changes in the eCBs that seem to facilitate alcohol consumption. In this study, we seek to evaluate potential morphological changes in neurons of the frontal cortex (FCx) and nucleus accumbens (NAcc), in the expression of receptors and enzymes of the endocannabinoid and dopamine systems and in second messengers, such as Akt, in adult rats subjected to MS and early stress (MS + ES; 2 × 180 min daily) vs. nonseparated rats (NMS). Results showed that MS + ES induces higher D2R expression and lower D3R, FAAH, and MAGL expression compared with NMS rats. Alterations in total dendritic length were also detected and were characterized by increases in the NAcc while there were decreases in the FCx. We believe MS + ES‐induced changes in the dopaminergic and endocannabinergic systems and in the neuronal microstructure might be contributing to alcohol seeking behavior and, potential vulnerability to other drugs in rats. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 819–831, 2016
Overview of low molecular weight heparin for preventative treatment of adverse obstetric outcomes related to abnormal placentation

Different proportions of cases of preterm and severe preeclampsia, placental abruption, fetal growth restriction, and fetal death share a common causal pathway of abnormal placental implantation. Documentation of an association between the risk of such adverse pregnancy outcomes (APOs) and inherited thrombophilias prompted initial studies to evaluate the benefit of anticoagulants for the prevention of recurrences both in patients with and without inherited thrombophilias. Prenatal administration of low molecular weight heparin (LMWH) has been evaluated in case control, cohort and randomized clinical trials. The evidence suggests a benefit of LMWH in the reduction of recurrences of APOs, with a number needed to treat of 6 (95% confidence interval: 4–10) to prevent one case of recurrent APOs. Such benefit is independent of the presence of inherited thrombophilias or the administration of low dose aspirin. Further studies are needed to establish the optimal duration for the prophylaxis, to better delineate the mechanism of action of LMWH and to explore the role, if any, of maternal serum markers and uterine artery Doppler findings in the modulation of the LMWH prophylaxis. © 2014 John Wiley & Sons, Ltd.
Chronic Q fever.

Sixteen cases of chronic Q fever are described. In eight there was a history of exposure to infection from farms or farm products. All had valvular heart disease, involving the mitral valve in nine and the aortic valve in seven. Infection occurred on a prosthetic valve in two patients. Arterial embolism was common. Venous thrombosis occured in three patients, and pulmonary embolism occurred in three other patients. Complement fixing antibodies to phase 1 antigen were found in a titre of 1:200 or greater in all except two patients. In one of these post-mortem examination revealed rickettsial bodies in mitral valve vegetations, and in the other Coxiella burneti was isolated from heart valve tissue. The majority presented with infective endocarditis but two presented primarily with liver disease. All patients had evidence of liver involvement and in one this led to death from cirrhosis. Abnormal tests of liver function, particularly hyperglobulinaemia, raised alkaline phsophatase and abnormal bromsulphthalein retention were found in all patients. Hepatic histology was abnormal in all eight patients in whom it was studied. The commonest features were mononuclear cell infiltration of the portal tracts and prominence of the sinusoidal Kupffer cells. Patchy focal necrosis of parenchymal cells, granulomata, fatty change, and eosinophilia of the sinusoidal walls were also noted in several patients and cirrhosis developed in one. Six patients had a purpuric rash, and in 12 there was thrombocytopenia. It is suggested that the presence of hepatomegaly and liver involvement and thrombocytopenia may help to differentiate Q fever endocarditis from bacterial endocarditis. Raised serum IgM and IgA levels occured frequently, but with only a moderate dominance of IgM. Sheep cell agglutination and latex fixation tests for rheumatoid factor were occasionally positive. Several features of the disease suggest the possibility that immune-complex mechanisms may play a role in chronic Q fever. Treatment was with prolonged courses of tetracycline usually combined with lincomycin. Seven patients underwent valve replacement surgery for haemodynamic reasons. Five patients died; two from heart failure, one from cirrhosis, one seven days after valve replacement and one from intraperitoneal haemorrhage following percutaneous liver biopsy. Three patients have survived for more than five years, and another six for more than three and a half years after diagnosis. Of these nine patients, three received medical therapy alone and six required valve replacement as well. Antibiotics have been discontinued in four patients who have had valve surgery and three others. Six patients had received antibiotics for continuous periods varying from 29-62 months. In the period after stopping therapy varying from 15-21 months, no relapse has occured. A seventh patient, who had received antibiotics for four months prior to valve replacement, has survived 43 months after the withdrawal of antibiotics.
Abuse Subtypes and Nonsuicidal Self-Injury: Preliminary Evidence of Complex Emotion Regulation Patterns

Research has identified complex relationships between abuse experiences, emotion regulation, and nonsuicidal self-injury (NSSI). Data generally indicate that individuals with an abuse experience, or those with NSSI, have difficulties with emotion regulation. However, it is unknown whether there are specific patterns of emotion regulation difficulties across abuse subtypes that are uniquely associated with engaging in NSSI. Using a sample of 2238 college students (n = 419; 18.1% with NSSI histories), the present study examined differences in emotion regulation difficulties across specific abuse types between those with and without a history of NSSI. Results indicate significantly greater difficulties with emotion regulation among abused and self-injuring participants as well as significant differences on specific emotion regulation problems between self-injuring and noninjuring participants within the physical abuse and combination physical/sexual abuse subtypes. Possible explanations and implications of these findings are discussed.
On the methods for the estimation of salinity tolerance of ciliates

Summary Critical review of research methods for tolerance and potential tolerance of ciliates is given. Using several Paramecium species as an example the original approaches to potential tolerance estimation is presented. Connection between resistance, tolerance and potential tolerance is shown. Model for explanation of the connection is proposed. Several application aspects of diagrams of the connection between acclimation salinity and value of upper salinity tolerant limit in the ciliates are considered.
Segmental Instability in Cervical Spondylotic Myelopathy With Severe Disc Degeneration

Study Design. A retrospective study was conducted. Objective. To investigate relationships between severe disc degeneration (SDD) and segmental instability in cervical spondylotic myelopathy (CSM) and to discuss surgical treatment for CSM with severe disc degeneration. Summary of Background Data. Information on relationships between segmental instability and spinal cord compression in CSM with severe disc degeneration is scarce. Methods. Radiographs and magnetic resonance images of patients with CSM with (n = 42) and without (n = 75) SDD were reviewed retrospectively. Cervical instability and spinal cord compression factors were analyzed. Outcomes of anterior cervical decompression and fusion (ACDF) and expansive laminoplasty (ELAP) were evaluated in medical records of follow-up clinics. Results. Segmental instability was found in 71.4% of patients with SDD and 22.7% of patients without SDD. Spinal cord compression was found at the intervertebral space of SDD and upper adjacent disc space. The recovery rate of ACDF and ELAP was 60.8% and 57.1%, respectively. Conclusion. The upper adjacent vertebra above SDD has inclination of segmental instability. There is static spinal cord compression in intervertebral spaces of SDD and dynamic compression in upper adjacent intervertebral spaces. Multilevel anterior cervical decompression and fusion or expansive laminoplasty should be used for surgical treatment.
U.S. Earnings Levels and Earnings Inequality: A Review of Recent Trends and Proposed Explanations

The article studies the U.S. earning trends since 1950 and gives explanations for the inequality in earnings. Both slow growth and increased inequality appear in the comparison of adult male earnings distributions for 1979 and 1987. Trends in women's earnings paint a somewhat brighter picture. Women, like men, have experienced slow hourly wage growth and growing wage inequality. But in terms of annual earnings, both factors have been offset by changes in hours worked. The result is a significant increase in the proportion of women who earn $20,000 a year or more. A combination of shifts in supply and shifts in demand is necessary to explain the observed trends between these groups. A critical aspect of supply shifts was the entry into the labor market of the well-educated baby boom generation. Demand shifts can be characterized as a long-term trend toward increasing relative demand for highly skilled workers. The growth in within group earnings inequality has many potential explanations, but it is not well understood and contains opportunities for future research.
Deconstructing Positive Affect in Later Life: A Differential Functionalist Analysis of Joy and Interest

Positive affect, an index of psychological well-being, is a known predictor of functionality and health in later life. Measures typically studied include joy, happiness, and subjective well-being, but less often interest—a positive emotion with functional properties that differ from joy or happiness. Following differential emotions theory, the present study measured trait joy and interest in a population-based sample of 1,118 adults aged 65–86 years. As predicted, trait joy was associated with greater religious participation, while trait interest was associated with greater education. Joy was associated with lower morbidity and stress while interest was not. Interest was, in fact, associated with greater stress. Both emotions were positively associated with social support. We use the pattern of predictors to develop a functionalist conceptualization of these two emotions in later life, concluding that it is worthwhile to treat interest and joy as partially-independent positive affects contributing differentially to human emotionality and later life adaptation.
Enlargement of human cerebral ischemic lesion volumes measured by diffusion‐weighted magnetic resonance imaging

We aimed to determine the frequency and time course of the enlargement of ischemic cerebral lesions following human stroke and to study the effect of the state of perfusion on lesion enlargement. Acute lesion volumes were measured on diffusion‐weighted magnetic resonance images and compared with lesion volumes measured on T2‐weighted images at 7 days or later. Forty‐four measurements were performed between 2 and 53 hours after stroke onset in 28 patients. Thirteen patients also had magnetic resonance perfusion imaging performed. In 12 (43%) of 28 patients the initial lesion volume increased by 20% or more. The number of studies showing enlargement of the ischemic lesion volume ranged from 12 (43%) of 28 at or after 2 hours to 10 (38%) of 26 at or after 6 hours, 5 (33%) of 15 at or after 24 hours, and 2 (33%) of 6 at or after 48 hours. In 7 of the 10 patients in whom the hypoperfusion volume acutely exceeded the volume of the abnormality on diffusion‐weighted images, lesion volume increased by 20% or more. This study provided evidence that substantial enlargement of human cerebral ischemic lesion volumes can occur beyond the first 6, 12 or 24 hours after onset. A mismatch acutely between the region of hypoperfusion (larger) and the region of diffusion abnormality (smaller) may be predictive of ischemic lesion enlargement.