Miracle-dz/UniFAR-FTUs huggingface dataset:
iNOS Activity Is Required for the Therapeutic Effect of Mesenchymal Stem Cells in Experimental Systemic Sclerosis

Objectives: Fibrosis is a hallmark of systemic sclerosis (SSc), an intractable disease where innovative strategies are still being sought. Among novel anti-fibrotic approaches, mesenchymal stromal/stem cell (MSC)-based therapy appears promising. Previously, we reported anti-fibrotic effects of MSC in an experimental model of SSc, through various mechanisms (tissue remodeling, immunomodulation, anti-oxidant defense). Since immunomodulation is a pivotal mechanism for MSC therapeutic effects, we investigated the specific role of critical molecules associated with MSC immunosuppressive properties and hypothesized that MSC defective for these molecules would be less effective in reducing fibrosis in SSc. Methods: SSc was induced by 6-week daily intradermal injections of hypochlorite (HOCl) in mice. MSC were isolated from the bone marrow of wild type mice (WT) or mice knockout for IL1RA, IL6, or iNOS (IL1RA−/−, IL6−/−, or iNOS−/− MSC, respectively). Treated-mice received 2.5 × 105 MSC intravenous infusion at d21. Skin thickness, histological and biological parameters were evaluated in skin and blood at d42. Results: IL1RA−/− and IL6−/− MSC exerted similar anti-fibrotic properties as WT MSC, with a reduction of skin thickness together with less collagen deposition. Conversely, iNOS−/− MSC did not exert anti-fibrotic functions as shown by a similar skin thickness progression as non-treated HOCl-SSc mice. Compared with WT MSC, iNOS−/− MSC kept some immunosuppressive and tissue remodeling properties, but lost their capacity to reduce oxidative stress in HOCl-SSc mice. Conclusion: Our study highlights the crucial role of iNOS, whose activity is required for the anti-fibrotic properties of MSC in experimental SSc, with a special emphasis on NO-related anti-oxidant functions.
Novel Antibiotic Testing Approaches Reveal Reduced Antibiotic Efficacy Against Shiga Toxin-Producing Escherichia coli O157:H7 Under Simulated Microgravity

As a foodborne and environmental pathogen, Shiga toxin-producing Escherichia coli O157:H7 could pose a health threat to immunocompromised astronauts during a space mission. In this study, novel approaches, including real-time testing and direct evaluation of resistance mechanisms, were used to evaluate antibiotic efficacy against E. coli O157:H7 under low-shear modeled microgravity (LSMMG) produced using a rotary cell culture system. When compared with normal gravity (NG), bacterial growth was increased under LSMMG in the presence of sub-inhibitory nalidixic acid concentrations and there was an accompanying up-regulation of stress-related genes. LSMMG also induced transcriptional changes of the virulence genes stx1 and stx2, highlighting the potential risk of inappropriate antibiotic use during a spaceflight. The degree of bacterial cell damage induced by the antibiotics was reduced under LSMMG, suggesting low induction of reactive oxygen species. Efflux pumps were also shown to play an important role in these responses. Increased cell filamentation was observed under LSMMG upon ampicillin treatment, possibly reflecting a protective mechanism against exposure to antibiotics. These observations indicate that, in the presence of antibiotics, the survival of E. coli O157:H7 is greater under LSMMG than under NG, indicating that antibiotic therapies may need to be adjusted during space missions.
Use of Radiomics Combined With Machine Learning Method in the Recurrence Patterns After Intensity-Modulated Radiotherapy for Nasopharyngeal Carcinoma: A Preliminary Study

Objective: To analyze the recurrence patterns and reasons in patients with nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiotherapy (IMRT) and to investigate the feasibility of radiomics for analysis of radioresistance. Methods: We analyzed 306 NPC patients treated with IMRT from Jul-2009 to Aug-2016, 20 of whom developed with recurrence. For the NPCs with recurrence, CT, MR, or PET/CT images of recurrent disease were registered with the primary planning CT for dosimetry analysis. The recurrences were defined as in-field, marginal or out-of-field, according to dose-volume histogram (DVH) of the recurrence volume. To explore the predictive power of radiomics for NPCs with in-field recurrences (NPC-IFR), 16 NPCs with non-progression disease (NPC-NPD) were used for comparison. For these NPC-IFRs and NPC-NPDs, 1117 radiomic features were quantified from the tumor region using pre-treatment spectral attenuated inversion-recovery T2-weighted (SPAIR T2W) magnetic resonance imaging (MRI). Intraclass correlation coefficients (ICC) and Pearson correlation coefficient (PCC) was calculated to identify influential feature subset. Kruskal-Wallis test and receiver operating characteristic (ROC) analysis were employed to assess the capability of each feature on NPC-IFR prediction. Principal component analysis (PCA) was performed for feature reduction. Artificial neural network (ANN), k-nearest neighbor (KNN), and support vector machine (SVM) models were trained and validated by using stratified 10-fold cross validation. Results: The median follow up was 26.5 (range 8–65) months. 9/20 (45%) occurred in the primary tumor, 8/20 (40%) occurred in regional lymph nodes, and 3/20 (15%) patients developed a primary and regional failure. Dosimetric and target volume analysis of the recurrence indicated that there were 18 in-field, and 1 marginal as well as 1 out-of-field recurrence. With pre-therapeutic SPAIR T2W MRI images available, 11 NPC-IFRs (11 of 18 NPC-IFRs who had available pre-therapeutic MRI) and 16 NPC-NPDs were subsequently employed for radiomic analysis. Results showed that NPC-IFRs vs. NPC-NPDs could be differentiated by 8 features (AUCs: 0.727–0.835). The classification models showed potential in prediction of NPC-IFR with higher accuracies (ANN: 0.812, KNN: 0.775, SVM: 0.732). Conclusion: In-field and high-dose region relapse were the main recurrence patterns which may be due to the radioresistance. After integration in the clinical workflow, radiomic analysis can be served as imaging biomarkers to facilitate early salvage for NPC patients who are at risk of in-field recurrence.
Spontaneous emission of semiconductors in the Wigner approach

This paper presents a first step towards combining two well-established methods used in semiconductor physics—semiconductor Bloch equations and the Wigner approach to quantum transport. This combination provides the possibility of including spontaneous emission, i.e., the spontaneous recombination of excited electron–hole pairs in semiconductors, into the Wigner approach, which so far has been used only for systems with fixed particle number. The theory is presented and first numerical results for a three-dimensional system are shown.
Sprint Interval Training and Continuous Aerobic Exercise Training Have Similar Effects on Exercise Motivation and Affective Responses to Exercise in Patients With Major Depressive Disorders: A Randomized Controlled Trial

Background: Sprint interval training (SIT) has become increasingly popular and is seen as a promising exercise strategy to increase fitness in healthy people. Nevertheless, some scholars doubt the appropriateness of a SIT training protocol for largely physically inactive populations. SIT might be too arduous, and therefore contribute to feelings of incompetence, failure, and lower self-esteem, which may undermine participants' exercise motivation. Therefore, we examined whether participation in 12 SIT sessions would lead to different changes in self-determined motivation, affective responses to exercise, cardiorespiratory fitness, physical activity, and depressive symptom severity compared to aerobic exercise training (CAT) in a sample of patients with major depressive disorders (MDD). Methods: Two groups of 25 patients (39 women, 11 men) with unipolar depression were randomly assigned to the SIT or CAT condition (M = 36.4 years, SD = 11.3). Data were assessed at baseline and post-intervention (three weekly 35-min sessions of SIT/CAT over a 4-week period). Self-determined exercise motivation was assessed with a 12-item self-rating questionnaire, affective valence was assessed in each session, prior, during, and after the exercise training using the Feeling Scale (FS). Cardiovascular fitness was measured with a maximal bicycle ergometer test, self-perceived fitness with a 1-item rating scale, physical activity with the International Physical Activity Questionnaire (IPAQ-SF), and depressive symptom severity with the Beck Depression Inventory II (BDi-II). Results: The SIT and CAT groups did not differ with regard to their changes in self-determined motivation from baseline to post-intervention. Participants in the SIT and CAT group showed similar (positive) affective responses during and after the training sessions. Cardiorespiratory fitness, self-perceived fitness and depressive symptom severity similarly improved in the SIT and CAT group. Finally, significant increases were observed in self-reported physical activity from baseline to post-intervention. However, these increases were larger in the CAT compared to the SIT group. Conclusion: From a motivational point of view, SIT seems just as suited as CAT in the treatment of patients with MDD. This is a promising finding because according to self-determination theory, it seems advantageous for patients to choose between different exercise therapy regimes, and for their preferences with regard to exercise type and intensity to be considered.
Assessment of Co-Morbidities and Self Esteem in Obese Population of Urban Slum of Mumbai

Obesity is perhaps the most prevalent form of malnutrition. As a chronic disease, prevalent in both developed and developing countries , and affecting both children and adults replacing the more traditional public health concerns including under-nutrition. For industrialized countries, it has been suggested that such increase in body weight have been caused primarily by reduced levels of physical activity, rather than by changes in food intake or other factors. The present study was carried out to assess the various co-morbidities associated with obesity and to assess self-esteem score of obese patients in an urban slum of Mumbai. A descriptive epidemiological study was conducted at Cheetah Camp urban slum which is a field practice area of Department of Preventive and Social Medicine, TN Medical College, Mumbai, India. Obese and overweight patients above 40 years of age were included in this study. The information was gathered by personal interview using semi-structured questionnaires. Out of 350 subjects 163 (46.57%) were males. Mean Self-esteem score was 19.47 Patients having high body mass index had poor control over blood pressure and blood sugar. Thus society for elderly should be formed where various life style changes to prevent geriatric problems including obesity and associated co-morbidities like diabetes, hypertension, etc. should be encouraged.
Underweight increases the risk of pulmonary tuberculosis in adult

Background Tuberculosis (TB) is a leading cause of morbidity and mortality, especially in middle- and low-income countries. The risk of developing TB may be related to nutritional status. Socioeconomic and behavioral factors are also shown to increase the susceptibility to TB infection. The objective of this study was to determine nutritional factors as risk factors of pulmonary TB in adult. Methods This was an observational study of case control design. The study subjects were community members consisting of 19 adult cases of pulmonary tuberculosis and 38 controls. Data on nutritional intakes were obtained by semiquantitative food frequency questionnaire (FFQ), while smoking behavior, history of DM, body mass index, education, and income were obtained by structured interviews. The data were analyzed by independent t-test and logistic regression for calculation of the odds ratio (OR). Results The bivariate test showed that the adequacy levels for energy (OR=6.8; 95% CI: 1.51-30.54), protein (OR=5.1; 95% CI: 1.52-17.14), vitamin A (OR=4.2; 5% CI: 1.31-13.54), vitamin C (OR=3.8;95% CI: 1.21-12.36), selenium (OR=4.2; 95% CI: 1.34-13.58), body mass index (OR=4.4; 95% CI: 1.32-14.35) and smoking behavior (OR=3.7; 95% CI: 1.15-11.9), were significant risk factors for pulmonary tuberculosis. Multiple logistic regression test showed that low body mass index (<18.5 kg/m2) (OR=6.0; 95% CI: 1.32-27.18) was a the most influential risk factor of pulmonary tuberculosis. Conclusion Low body mass index is the most influential risk factor for pulmonary tuberculosis incidence in adult. Nutrition profile in adult is an important determinant of TB incidence.
Interpersonal Influences on Body Representations in the Infant Brain

Within cognitive neuroscience, there is burgeoning interest in how the body is represented in the adult brain. However, there are large gaps in the understanding of neural body representations from a developmental perspective. Of particular interest are the interconnections between somatosensation and vision, specifically infants’ abilities to register correspondences between their own bodies and the bodies of others. Such registration may play an important role in social learning and in engendering feelings of connectedness with others. In the current study, we further explored the interpersonal aspects of neural body representations by examining whether responses to tactile stimulation in 7-month-old infants are influenced by viewing another’s body. During EEG recording, infants (N= 60) observed a live presentation of an experimenter’s hand or foot being touched. During the presentation of touch to the adult’s hand or foot, the infant received a brief tactile touch to their right hand or right foot. This resulted in four conditions: (i) receive hand stimulation/observe hand stimulation, (ii) receive hand stimulation/observe foot stimulation, (iii) receive foot stimulation/observe hand stimulation, and (iv) receive foot stimulation/observe foot stimulation. Analyses compared responses overlying hand and foot regions when the observed limb matched the stimulated limb (congruent) and did not match (incongruent). In line with prior work, tactile stimulation elicited a somatotopic pattern of results in the somatosensory evoked potential (SEP) and the sensorimotor mu rhythm (6–9 Hz). Cross-modal influences were observed in the beta rhythm (11–13 Hz) response and in the late potential of the SEP response (400–600 ms). Beta desynchronization was greater for congruent compared to incongruent conditions. Additionally, tactile stimulation to the foot elicited larger mean amplitudes for congruent compared to incongruent conditions. The opposite was true for stimulation to the hand. This set of novel findings suggests the importance of considering cross-modal effects in the study of neural body representations in the infant brain. Continued work in this new area of infant neuroscience research can inform how interpersonal aspects of body representations may serve to undergird early social learning.
Analysis of Bacterial Community Characteristics, Abundance of Antibiotics and Antibiotic Resistance Genes Along a Pollution Gradient of Ba River in Xi’an, China

The microbial communities in freshwater have raised concerns about the ecosystem and human health. Many ecological environmental problems have been found in urban river because of the unreasonable use and long-term wastewater discharge. In this study, we explored the bacterial community composition, abundance of 14 antibiotics and 21 antibiotic resistance genes (ARGs), and water environment features in seven water samples and seven sediment samples from Ba River in Xi’an, China. Results showed Proteobacteria and Bacteroidetes were the dominant phyla in all samples, and sediment samples had a higher bacterial diversity and richness than it in water. Bacterial communities of site 5 and 6 were clustered in discrepant patterns compared to those at remaining sites from other samples. It might be influenced by nutrients, heavy metals and antibiotics. Antibiotics concentrations ranged from 1.26 to 1.61 × 103 ng L-1 in water samples and 1.55 to 4.05 × 102 μg kg-1 in sediment samples. Sulfamerazine (SM1) and erythromycin (ERY) were the chief antibiotics in water samples, while the level of oxytetracycline (OTC) and cefazolin (CFZ) were higher in sediment samples. Canonical correspondence analysis showed that trimethoprim (TMP) was significantly related to Acinetobacter in W6, and that SM1 and OTC had positive correlation with Arcobacter in W5. The tetC, blaTEM, ermF and sul1 had higher pollution abundance ranging from 10-4 to 100 copies/16S rRNA gene copies in all samples. Significant correlations were observed between ARGs and matching antibiotics, suggesting that antibiotics can pose the selective pressure on ARGs in this river. In summary, these finding might provide some new data to the limited information available on the bacterial community characteristics, abundance of antibiotics and ARGs in urban river of China.
A Systematic DFT Approach for Studying Mechanisms of Redox Active Enzymes

When DFT has been applied to study mechanisms of redox processes a common procedure has been to study the results for many different functionals. For redox reactions involving the first row transition metals, this approach has given very different results for different functionals. The conclusion has been that DFT cannot be used for these reactions. In the meantime, results with strong predictability have been generated, most noteworthy for photosystem II, where all DFT predictions have been verified by experiments performed later. In order to obtain these predictive results using DFT, an alternative, systematic approach has been used, where the key differences between the results for different functionals can be rationalized by using a single parameter, rather than using the very large number of differences in the functionals.
A Unified Framework for Solving Multiagent Task Assignment Problems

Abstract : This research presents a unified approach to representing and solving the multiagent task assignment problem for complex problem domains using ideas central to multiagent task allocation, project scheduling, constraint satisfaction, and coalition formation, forming the basis of the constrained multiagent task scheduling (CMTS) problem. The CMTS descriptor represents a wide range of classical and modern problems, such as job shop scheduling, the traveling salesman problem, vehicle routing, and cooperative multi-object tracking. Problems using the CMTS representation are solvable by a suite of algorithms ranging from simple random scheduling to state-of-the-art biologically inspired approaches incorporating evolutionary algorithms, dynamic coalition formation, auctioning, and behavior-based robotics to highlight different solution generation strategies. The framework includes a distributed process to show how to scale adapted algorithms to solve increasingly larger domain problems. This approach introduces several methods for problem decomposition and recomposition without significantly compromising solution quality. Decomposition techniques show methods to reduce the search space by several orders of magnitude allowing for improved search efficiency.
Successive Oligopolies with Differentiated Firms and Endogeneous Entry

We develop a model of successive oligopolies with endogenous entry, allowing for varying degrees of product differentiation and entry costs in both markets. We show that downstream conditions dominate the overall profitability of the two-tier structure while upstream conditions mainly affect the distribution of profits. We analyze how two-part tariffs and resale price maintenance shape the endogenous market structure and study their welfare effects. In contrast to previous literature, we find that welfare under linear prices can be larger than under twopart tariffs although the latter avoids double marginalization. This is because linear prices induce more downstream market entry.
Increasing Interpersonal Interactions in an Online Course: Does Increased Instructor Email Activity and Voluntary Meeting Time in a Physical Classroom Facilitate Student Learning?.

Distance learning is expanding rapidly in universities. While theoretical and qualitative literature stress the critical role of effective interpersonal interactions in motivating students and facilitating learning in online environments, quantitative evidence on the benefits of increased interpersonal interactions on student learning outcomes is limited. This study examines the effect of providing a voluntary in-person meeting time in a physical classroom and increasing instructor email activity in a fully online precalculus course at a public university. We examine student final exam score and course grade as outcome variables. Student selection into courses was minimal since students only had access to one treatment condition at a time. We further used a propensity score matching strategy to address demographic variations in student characteristics across cohorts. Our results indicate that the increased interpersonal interaction opportunities increased final exam scores by 0.22 standard deviations and improved passing rates by 19 percentage points. Rosenbaum’s sensitivity analysis indicates that it is unlikely that these results are due to omitted variable bias.
TIBIAL ROTATIONS DURING STEP UP EXERCISE DO NOT CHANGE KNEE EXTENSOR ACTIVITY IN THE PATELLOFEMORAL PAIN SYNDROME

The purpose of this study was to compare the electromyographic activity of vastus medialis (VM) and vastus lateralis (VL) muscles during step up exercises at different tibial rotations, between individuals with and without patellofemoral pain syndrome. The electromyographic activity of the VM and VL muscles during step up exercises was evaluated in three tibial rotations: without rotation, medial and lateral rotation. Thirteen patients with patellofemoral pain syndrome and 16 control subjects performed this exercise three times. The results showed a significant lower electromyographic activity of VM muscle in both groups, compared to the electromyographic activity of VL muscle (p<0.05). Moreover, a significant difference in electromyographic activity between muscles for all rotations and groups (p<0.001) was also found. The results of the present study suggest that tibial rotations performed during step up exercise did not change the electromyographic activity of VM and VL muscles.
Student perceptions of assessment and student self-efficacy in competence-based education

The purpose of this study was to provide insight into the interplay between student perceptions of competence-based assessment and student self-efficacy, and how this influences student learning outcomes. Results reveal that student perceptions of the form authenticity aspect and the quality feedback aspect of assessment do predict student self-efficacy, confirming the role of mastery experiences and social persuasions in enhancing student self-efficacy as stated by social cognitive theory. Findings do not confirm mastery experiences as being a stronger source of self-efficacy information than social persuasions. Study results confirm the predictive role of students’ self-efficacy on their competence outcomes. Mediation analysis results indicate that student’s perceptions of assessment have an indirect effect on student’s competence evaluation outcomes through student’s self-efficacy. Study findings highlight which assessment characteristics, positively influencing students’ learning, contribute to the effectiveness of competence-based education. Limitations of the study and directions for future research are indicated.