Miracle-dz/UniFAR-FTUs huggingface dataset:
Clinical improvement in chronic plaque‐type psoriasis lesions after narrow‐band UVB therapy is accompanied by a decrease in the expression of IFN‐γ inducers – IL‐12, IL‐18 and IL‐23

Abstract: Type‐1 cytokine‐producing T cells are important in the pathogenesis of psoriasis vulgaris, for which efficient therapy is provided by means of narrow‐band ultraviolet‐B (NB‐UVB). The expression of the type‐1 cytokine interferon‐γ (IFN‐γ) is regulated by interleukin‐12 (IL‐12), IL‐15, IL‐18 and IL‐23; however, not much is known about the effect of this therapy on the levels of these cytokines in lesional psoriatic skin in situ. In this study, we investigated the effects of NB‐UVB therapy on the expression of IFN‐γ‐inducing cytokines. Ten patients with chronic plaque‐type psoriasis selected to be treated with NB‐UVB therapy were recruited for these experiments and the expression of cytokines IL‐12, IL‐15, IL‐18, IL‐23 and IFN‐γ in lesional psoriatic skin before, during and after therapy was determined with the help of immunohistochemistry. Double staining was performed in order to determine the cell types expressing these cytokines. The decrease in the psoriasis area and severity index was accompanied by a significant decrease in the expression of IFN‐γ, and concomitantly, significant reduction of IFN‐γinducers – IL‐12, IL‐18 and IL‐23. Thus, we concluded that the decrease of IFN‐γ expression in psoriasis lesions after NB‐UVB therapy could be a result of diminished expression of IL‐12, IL‐18 and IL‐23 in lesional skin. Therapies targeting these three cytokines should, therefore, be considered in the treatment of psoriasis.
A prospective study of acute poisonings in a sample of Greek patients.

OBJECTIVES Acute poisoning is one of the most common situations managed in the emergency department. Significant differences have been reported concerning the prevalence and etiology of poisoning. This study aims to present the epidemiologic and clinical characteristics of poisonings in the region of Thrace in Northern Greece, where no similar studies have been performed. METHODS A prospective study was carried out in our hospital's emergency department during 4 years (1999-2003). Atotal of 223 adult patients were admitted with acute poisoning, 87 males (39%) and 136 females (61%). RESULTS Mean age of male and female patients was 37.1 years (standard deviation 16.1) and 33.4 years (standard deviation 14.5), respectively. Intoxications were more common in summer (34.1%). The median time between poisoning and presentation was 4 hours. Poisonings were more common in the urban population (64.1%). Psychotropes were the leading cause of poisoning in patients with a psychiatric disease (74.1%) and analgesics in all the other patients groups (34.8%). The clinical status on admission was: conscious (45.7%), confused (35.4%), precoma/coma (18.9%). Haemodialysis was performed only in one patient and respiratory support was necessary in 10 patients. An antidote was administrated in 73 patients. Median hospital stay was 1 day. Two patients died (0.9%). CONCLUSIONS Poisoning's aetiology varies significantly in different countries. Previous suicide attempts are common in these patients. Precipitating factors can be major depressive, or dysthymic disorders. The mortality rate is low.
Multiple glucose 6-phosphate dehydrogenase-deficient variants correlate with malaria endemicity in the Vanuatu archipelago (southwestern Pacific).

In studying the relationship between genetic abnormalities of red blood cells and malaria endemicity in the Vanuatu archipelago in the southwestern Pacific, we have found that of 1,442 males tested, 98 (6.8%) were G6PD deficient. The prevalence of GdPD deficiency varied widely (0%-39%), both from one island to another and in different parts of the same island, and generally correlated positively with the degree of malaria transmission. The properties of G6PD from GdPD-deficient subjects were analyzed in a subset of 53 samples. In all cases the residual red-blood-cell activity was < 10%. There were three phenotypic patterns. PCR amplification and sequencing of the entire coding region of the G6PD gene showed that the first of these patterns corresponded to G6PD Union (nucleotide 1360C-->T; amino acid 454Arg-->Cys), previously encountered elsewhere. Analysis of samples exhibiting the second pattern revealed two new mutants: G6PD Vanua Lava (nucleotide 383T-->C; amino acid 128Leu-->Pro) and G6PD Namoru (nucleotide 208T-->C; amino acid 70Tyr-->His); in three samples, the underlying mutation has not yet been identified. Analysis of the sample exhibiting the third pattern revealed another new mutant: G6PD Naone (nucleotide 497G-->A; amino acid 166Arg-->His). Of the four mutations, G6PD Union and G6PD Vanua Lava have a polymorphic frequency in more than one island; and G6PD Vanua Lava has also been detected in a sample from Papua New Guinea. G6PD deficiency is of clinical importance in Vanuatu because it is a cause of neonatal jaundice and is responsible for numerous episodes of drug-induced acute hemolytic anemia.
Asymmetric Amplitude Modulations of Brain Oscillations Generate Slow Evoked Responses

Electrophysiological data measured by electroencephalography and magnetoencephalography (MEG) are widely used to investigate human brain activity in various cognitive tasks. This is typically done by characterizing event-related potentials/fields or modulations of oscillatory activity (e.g., event-related synchronization) in response to cognitively relevant stimuli. Here, we provide a link between the two phenomena. An essential component of our theory is that peaks and troughs of oscillatory activity fluctuate asymmetrically; e.g., peaks are more strongly modulated than troughs in response to stimuli. As a consequence, oscillatory brain activity will not “average out” when multiple trials are averaged. Using MEG, we demonstrate that such asymmetric amplitude fluctuations of the oscillatory alpha rhythm explain the generation of slow event-related fields. Furthermore, we provide a physiological explanation for the observed asymmetric amplitude fluctuations. In particular, slow event-related components are modulated by a wide range of cognitive tasks. Hence, our findings provide new insight into the physiological basis of cognitive modulation in event-related brain activity.
Evidence of exogenous reinfection and mixed infection with more than one strain of Mycobacterium tuberculosis among Spanish HIV-infected inmates.

BACKGROUND As prisons have a high prevalence of tuberculosis and HIV infection, we studied the possibility of multiple tuberculosis infections in a selected population of HIV-infected inmates. METHODS Two groups of patients with special characteristics were selected from 226 HIV-infected inmates diagnosed with tuberculosis in the prisons of Madrid (Spain) between 1993 and 1994. The first group contained nine patients who remained culture positive 4 months after the initiation of therapy and the second group contained 28 patients with Mycobacterium tuberculosis isolated from different anatomical sites. DNA typing with IS6110 was performed on all isolates from these patients. In some patients a secondary DNA typing with the plasmid pTBN12 was performed. RESULTS Two patients from group A had a second M. tuberculosis strain obtained 4 and 18 months after the initial isolate, with different IS6110 and pTBN12 patterns. In one patient the second strain was multidrug resistant and in the other patient both strains had the same drug-susceptible pattern. The clinical and microbiologic evidence in both patients was consistent with the presence of active tuberculosis caused by a new strain of M. tuberculosis. In group B, the isolates from 27 patients shared similar fingerprint pattern; however, in one patient isolates from sputum and urine showed different IS6110 and pTBN12 patterns, although both strains showed the same drug-susceptible phenotype. CONCLUSION This study provides evidence that HIV-infected inmates living under conditions of high environmental infectivity can be infected with two different strains of M. tuberculosis. This finding has implications for the tuberculosis-control programs in prison.
INFECTIOUS DISEASE AND TOXICOLOGICAL MONITORING OF STRANDED PACIFIC HARBOR SEALS (PHOCA VITULINA RICHARDSI) IN COOK INLET AS SURROGATES FOR MONITORING ENDANGERED BELUGAS (DELPHINAPTERUS LEUCAS)

Abstract: Pacific harbor seals (Phoca vitulina richardsi) and belugas (Delphinapterus leucas) eat many of the same prey species, occupy the same geographic area, and demonstrate site fidelity in Cook Inlet, Alaska. Although most direct research involving the critically endangered belugas is currently prohibited, studying harbor seals may provide important information about this beluga population. In recent years, harbor seal populations in Alaska have declined for unknown reasons. As part of its stranding program, the Alaska SeaLife Center (ASLC) managed 59 cases of live and dead stranded harbor seals from Cook Inlet between 1997 and 2011. Animals were screened for a variety of diseases and contaminants of concern. Animals were negative by serology to the following diseases: avian influenza, canine distemper virus, dolphin morbillivirus, porpoise morbillivirus, Leptospira canicola, L. grippotyphosa, L. pomona, Neospora caninum, Sarcocystis neurona, and Toxoplasma gondii. Positive titers were found against Brucella spp. , phocine distemper virus, seal herpesvirus-1, L. bratislava, L. hardjo, and L. icterohemorrhagiae. All titers were stable or declining except in one animal with an increasing titer for seal herpesvirus-1. Fecal pathogen screenings identified normal flora as well as stable or declining low levels of potentially pathogenic and opportunistic bacteria, though most were of little concern for seal health. In most animals, toxicology screening showed that the majority of tested contaminants were below detectable limits. The level of evidence of exposure to pathogens of concern was low in harbor seals. Although the infectious disease burden and contaminant levels in belugas in Cook Inlet cannot be definitively determined without direct testing, pathogen and contaminant exposure is expected to be similar to that found in harbor seals in this region, as the harbor seals and belugas share the habitat and food resources.
Safety and efficacy of administering 0.2 mg of recombinant human TSH for two consecutive days as an adjuvant to therapy with low radioiodine doses in elderly out-patients with large nontoxic multinodular goiter.

AIM The aim of this study was to evaluate the efficacy of recombinant human TSH (rhTSH) as an adjuvant to radioiodine therapy for nontoxic multinodular goiter (MNG) in elderly subjects. METHODS Twelve elderly out-patients with large MNG (group 1) were studied. The effect of adjuvant rhTSH administration (0.2 mg i.m. on 2 consecutive days) before low-dose (131)I was compared with that of radioiodine alone in 8 out-patients matched for age and MNG volume (group 2). The follow-up period was similar in both groups. RESULTS The number and severity of side-effects during the first month of treatment were similar in both groups. On final examination, the number of patients symptomatic for goiter was significantly lower in group 1 than in group 2 (P=0.03). In group 1, TSH levels peaked at 40.3+/-9.5 mU/L on day 3, from the baseline value of 0.5+/-0.1 mU/L (P<0.001). In group 2, baseline TSH was 0.4+/-0.1 mU/L. Although a marked increase in f-T3, f-T4 and Tg (P<0.001) was noted in both groups during the first 2 weeks of treatment, peak values were much higher in group 1 than in group 2. On final examination, a slightly significant increase (P=0.01) in TSH levels from the baseline was noted in both groups (group 1: 1.2+/-0.2 mU/L; group 2: 1.4+/-0.3 mU/L). The percentage of patients who did not need therapies to control TSH secretion at the last examination was higher in group 1 (83%) than in group 2 (38%). Only in group 1, a significant reduction was noted in mean anterior-posterior lobar width (31+/-1.7 mm) from the baseline value (24.5+/-1.7 mm, P=0.04). Thyroid volume was reduced from 78.1+/-11.7 mL to 49.4+/-13.4 mL (P=0.001) in group 1 and from 89.8+/-25.2 mL to 67.1+/-20.5 mL (P=0.04) in group 2. Six months after (131)I therapy, slight changes in thyroid length and tracheal lumen were noted in both groups. CONCLUSIONS This long-term controlled study demonstrates that 0.2 mg of rhTSH on 2 consecutive days increases the efficacy of ambulatory (131)I dosages in treating nontoxic MNG in elderly subjects. Adequate drug preparation generally prevents side-effects due to short-term but marked thyrotoxicosis that is aggravated by rhTSH administration. An increase in thyroid volume reduction seems to be the most important effect of rhTSH administered before (131)I.
Unexpected Opioid Activity Profiles of Analogues of the Novel Peptide Kappa Opioid Receptor Ligand CJ‐15,208

An alanine scan was performed on the novel κ opioid receptor (KOR) peptide ligand CJ‐15,208 to determine which residues contribute to the potent in vivo agonist activity observed for the parent peptide. These cyclic tetrapeptides were synthesized by a combination of solid‐phase peptide synthesis of the linear precursors, followed by cyclization in solution. Like the parent peptide, each of the analogues exhibited agonist activity and KOR antagonist activity in an antinociceptive assay in vivo. Unlike the parent peptide, the agonist activity of the potent analogues was mediated predominantly, if not exclusively, by μ opioid receptors (MOR). Thus analogues 2 and 4, in which one of the phenylalanine residues was replaced by alanine, exhibited both potent MOR agonist activity and KOR antagonist activity in vivo. These peptides represent novel lead compounds for the development of peptide‐based opioid analgesics.
Is inequality a political choice?

Is economic inequality the result of conscious policy choices? Or are growing inequalities the inevitable by-product of international trade, economic growth, structural changes in the economy, demographic changes, or other factors outside the scope of politics? By examining the effects of non-political forces on government redistribution of income within 16 OECD countries in the 1980-2000 period, this paper concludes that although international trade appears to reduce redistribution, high degrees of unionization maintain a significant positive impact. Of the parameters included in this study unionization and international trade appears to have the strongest effects on government inequality reduction, statistically significant even when controlling for fixed countryand year effects (Linear Mixed Model analysis). However, the data is inconclusive with respect to government partisanship’s effect on redistribution, and indicates that minority governments tend to reduce inequalities significantly more than majority governments. The paper ends with a remark about the need to separate empirical and normative arguments in studies of economic inequality.
Duodenal Microflora in Very-Low-Birth-Weight Neonates and Relation to Necrotizing Enterocolitis

ABSTRACT Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in the neonatal period. Small-bowel overgrowth with aerobic gram-negative bacteria has previously been implicated in the development of NEC. This prospective study performed quantitative bacteriology on 422 duodenal aspirates collected from 122 very-low-birth-weight (<1,500-g) newborns, at the time of routine changing of nasogastric tubes. Isolates ofEnterobacteriaceae were typed by repetitive extragenic, palindromic PCR and pulsed-field gel electrophoresis. One or more samples from 50% of these infants yielded gram-negative bacteria, predominantly Escherichia coli, Klebsiellaspp. , and Enterobacter spp. , with counts up to 108 CFU/g. The proportion of samples with gram-negative bacteria increased with postnatal age, while the percentage of sterile samples declined. Molecular typing revealed marked temporal clustering of indistinguishable strains. All infants had been fed prior to isolation of gram-negative organisms. Antibiotic use had no obvious effect on colonization with Enterobacteriaceae. There were 15 episodes of suspected NEC (stage I) and 8 confirmed cases of NEC (2 stage II and 6 stage III) during the study period. Duodenal aspirates were collected prior to clinical onset in 13 episodes of NEC. Seven of these yielded Enterobacteriaceae, of which five strains were also isolated from infants without NEC. Very-low-birth-weight infants have high levels of duodenal colonization withEnterobacteriaceae, with evidence of considerable cross-colonization with indistinguishable strains. There was no association between duodenal colonization with particular strains ofEnterobacteriaceae and development of NEC.
Medical Schools' Attitudes and Perceptions Regarding the Use of Central Institutional Review Boards

Purpose. To investigate the current practices, attitudes, perceptions, and future plans of U.S. medical schools regarding the use of central institutional review boards (IRBs) to review research involving human participants. Method. In 2003, a survey instrument was distributed via fax and e-mail to the deans of research at the 125 accredited U.S. medical schools. Each dean was asked to have the instrument completed by the official at that school who decided on the use of a central versus local IRB. The survey instrument consisted primarily of a variety of closed-ended questions. Results. Eighty-eight medical schools (69.8%) completed the instrument; 76% of these indicated that they had never used a central IRB and 24% had used a central IRB. Most of the respondents expressed no interest in using a central IRB in the future because they believed that their local IRB was working efficiently, and they were concerned about issues of institutional liability and the loss of local representation in the review process. Of the medical schools that had used a central IRB, most were pleased with the performance of the central IRB and would continue to use a central IRB in the future. Of interest, most of these respondents did not agree that a central IRB had helped them to attract industry-sponsored research. Conclusions. In spite of much discussion about the advantages of central IRBs in expediting overview of human subjects research, especially in multicenter trials, the majority of medical schools surveyed had never used a central IRB and expressed no interest in doing so.
Effect of dietary fiber intake on blood pressure: a meta-analysis of randomized, controlled clinical trials

Objective We conducted a meta-analysis of 25 randomized controlled trials published in English-language journals before February 2004, to assess the effect of dietary fiber intake on blood pressure (BP). Design Using a standardized protocol, information on study design, sample size, participant characteristics, duration of follow-up and change in mean BP, was abstracted. The data from each study were pooled using a random effects model to provide an overall estimate of dietary fiber intake on BP. Intervention Dietary fiber intake was the only significant intervention difference between the active and control groups. Results Overall, dietary fiber intake was associated with a significant −1.65 mmHg [95% confidence interval (CI), −2.70 to −0.61] reduction in diastolic BP (DBP) and a non-significant −1.15 mmHg (95% CI, −2.68 to 0.39) reduction in systolic BP (SBP). A significant reduction in both SBP and DBP was observed in trials conducted among patients with hypertension (SBP −5.95 mmHg, 95% CI, −9.50 to −2.40; DBP −4.20 mmHg, 95% CI, −6.55 to −1.85) and in trials with a duration of intervention ≥ 8 weeks (SBP −3.12 mmHg, 95% CI, −5.68 to −0.56; DBP −2.57 mmHg, 95% CI, −4.01 to −1.14). Conclusions Our results indicate that increased intake of dietary fiber may reduce BP in patients with hypertension and suggests a smaller, non-conclusive, reduction in normotensives. An intervention period of at least 8 weeks may be necessary to achieve the maximum reduction in BP. Our findings warrant conduct of additional clinical trials with a larger sample size and longer period of intervention to examine the effect of dietary fiber intake on BP.
Different Molecular Mechanisms for Rho Family GTPase-dependent, Ca2+-independent Contraction of Smooth Muscle*

Abnormal smooth muscle contraction may contribute to diseases such as asthma and hypertension. Alterations to myosin light chain kinase or phosphatase change the phosphorylation level of the 20-kDa myosin regulatory light chain (MRLC), increasing Ca2+ sensitivity and basal tone. One Rho family GTPase-dependent kinase, Rho-associated kinase (ROK or p160ROCK) can induce Ca2+-independent contraction of Triton-skinned smooth muscle by phosphorylating MRLC and/or myosin light chain phosphatase. We show that another Rho family GTPase-dependent kinase, p21-activated protein kinase (PAK), induces Triton-skinned smooth muscle contracts independently of calcium to 62 ± 12% (n = 10) of the value observed in presence of calcium. Remarkably, PAK and ROK use different molecular mechanisms to achieve the Ca2+-independent contraction. Like ROK and myosin light chain kinase, PAK phosphorylates MRLC at serine 19 in vitro. However, PAK-induced contraction correlates with enhanced phosphorylation of caldesmon and desmin but not MRLC. The level of MRLC phosphorylation remains similar to that in relaxed muscle fibers (absence of GST-mPAK3 and calcium) even as the force induced by GST-mPAK3 increases from 26 to 70%. Thus, PAK uncouples force generation from MRLC phosphorylation. These data support a model of PAK-induced contraction in which myosin phosphorylation is at least complemented through regulation of thin filament proteins. Because ROK and PAK homologues are present in smooth muscle, they may work in parallel to regulate smooth muscle contraction.
Electromagnetic Modeling of High-Frequency Links With High-Resolution Terrain Data

A quasi-deterministic model for the analysis of the electromagnetic propagation and estimation of main link parameters in fixed microwave and millimeter-wave links operating within urban or suburban environments is presented. The propagation geometry is modeled by a triangular mesh from the transformation of high-resolution LIDAR data. Subsequently, an efficient physical optics approximation is employed for the estimation of the mean scattered field power from surfaces within the main beams of the antennas. Random realizations of principal reflected and scattered fields constituents superimposed to the direct field allow for the prediction of worst-case link performance, in terms of outage probability, power delay spread, and cross-polar discrimination, for different design parameters: antenna position, gain, and height. The computations are compared to measurements of fixed links at 40 GHz.
Development of the Drosophila mushroom bodies: elaboration, remodeling and spatial organization of dendrites in the calyx

One Drosophila mushroom body (MB) is derived from four indistinguishable cell lineages, development of which involves sequential generation of multiple distinct types of neurons. Differential labeling of distinct MB clones reveals that MB dendrites of different clonal origins are well mixed at the larval stage but become restricted to distinct spaces in adults. Interestingly, a small dendritic domain in the adult MB calyx remains as a fourfold structure that, similar to the entire larval calyx, receives dendritic inputs from all four MB clones. Mosaic analysis of single neurons demonstrates that MB neurons, which are born around pupal formation, acquire unique dendritic branching patterns and consistently project their primary dendrites into the fourfold dendritic domain. Distinct dendrite distribution patterns are also observed for other subtypes of MB neurons. In addition, pruning of larval dendrites during metamorphosis allows for establishment of adult-specific dendrite elaboration/distribution patterns. Taken together, subregional differences exist in the adult Drosophila MB calyx, where processing and integration of distinct types of sensory information begin.