Miracle-dz/UniFAR-FTUs huggingface dataset:
Evidence of Alternative Splicing as a Regulatory Mechanism for Kissr2 in Pejerrey Fish

Kisspeptin receptors are G-Protein-Coupled Receptors that regulate GnRH synthesis and release in vertebrates. Here, we report the gene structure of two kisspeptin receptors (kissr2 and kissr3) in pejerrey fish. Genomic analysis exposed a gene structure with 5 exons and 4 introns for kissr2 and 6 exons and 5 introns for kissr3. Two alternative variants for both genes, named kissr2_v1 and _v2, and kissr3_v1 and v2, were revealed by gene expression analyses of several tissues. For both receptors, these variants were originated by alternative splicing retaining intron 3 and intron 4 for kissr2_v2 and kissr3_v2, respectively. In the case of kissr2, the intron retention introduced two stop codons leading to a putatively truncated protein whereas for kissr3, the intron retention produced a reading shift leading to a stop codon in exon 5. Modeling and structural analysis of Kissr2 and Kissr3 spliced variants revealed that truncation of the proteins may lead to non-functional proteins, as the structural elements missing are critical for receptor function. To understand the functional significance of splicing variants, the expression pattern for kissr2 was characterized on fish subjected to different diets. Fasting induced an up-regulation of kissr2_v1 in the hypothalamus, a brain region implicated in control of reproduction and food intake, with no expression of kissr2_v2. On the other hand, fasting did not elicit differential expression in testes and habenula. These results suggest that alternative splicing may play a role in regulating Kissr2 function in pejerrey.
Vocal and visual attractiveness are related in women

Abstract We investigated the relation between visual and vocal attractiveness in women as judged by men. We recorded 34 women speaking four vowels and measured the peak frequency, the first five harmonic frequencies, the first five formant frequencies and formant dispersion. The women were also photographed (head shot), several body measures were taken and their ages were recorded. The voices were played to male judges who were asked to assess the women's age and vocal attractiveness from the recording. The men were then asked to assess the attractiveness of the photographs. Men were in strong agreement on which was an attractive voice and face; and women with attractive faces had attractive voices. Higher-frequency voices were assessed as being more attractive and as belonging to younger women (the lowest frequency produced is a good indicator of age in women in general). Larger women had lower voices and were judged as having less attractive faces and voices. Taller women had narrower formant dispersion as predicted. The results imply that different measures of attractiveness are in agreement and signal similar qualities, such as female age, body size and possibly hormonal profile. Copyright 2003 Published by Elsevier Science Ltd on behalf of The Association for the Study of Animal Behaviour.
Identification and Expression Analyses of SBP-Box Genes Reveal Their Involvement in Abiotic Stress and Hormone Response in Tea Plant (Camellia sinensis)

The SQUAMOSA promoter binding protein (SBP)-box gene family is a plant-specific transcription factor family. This family plays a crucial role in plant growth and development. In this study, 20 SBP-box genes were identified in the tea plant genome and classified into six groups. The genes in each group shared similar exon-intron structures and motif positions. Expression pattern analyses in five different tissues demonstrated that expression in the buds and leaves was higher than that in other tissues. The cis-elements and expression patterns of the CsSBP genes suggested that the CsSBP genes play active roles in abiotic stress responses; these responses may depend on the abscisic acid (ABA), gibberellic acid (GA), and methyl jasmonate (MeJA) signaling pathways. Our work provides a comprehensive understanding of the CsSBP family and will aid in genetically improving tea plants.
Active accumulation of spawning substrate: a determinant of extreme polygyny in a shell-brooding cichlid fish

Abstract Abstract. Observations on the reproductive behaviour and polygynous mating system of an endemic shell-brooding cichlid fish, Lamprologus callipterus , revealed that territorial males actively transported and accumulated spawning substrate (empty gastropod shells) to make nests. In contrast to conventional resource defence polygyny, males of this species can therefore create a defensible resource distribution. Females successively visited the nests, chose one shell, spawned the whole clutch inside the shell and stayed inside until the young became independent. Males were freed from direct parental care but defended the territory. Nest usurpation by larger males and frequent shell stealing among males were observed; these resulted in cannibalism of the young fathered by the former males. Large males accumulated more shells and consequently monopolized more females than did smaller males. Maximum size of females was apparently limited by the size of available shells, resulting in extreme sexual dimorphism. Extreme polygyny in this species, associated with intense intra-sexual competition and extreme sexual dimorphism, was based mainly on transportability of spawning substrate and the specific behavioural competence of males to use it.
Care enough to cheat? How lay beliefs of self-mastery guide unethical behavior

http://repository.ust. hk/ir/ This version is available at HKUST Institutional Repository via If it is the author’s pre-published version, changes introduced as a result of publishing processes such as copy-editing and formatting may not be reflected in this document. For a definitive version of this work, please refer to the published version. Care enough to cheat? How lay beliefs of self-mastery guide unethical behavior Johar, Gita Venkataramani; Sengupta, Jaideep; Mukhopadhyay, Anirban
Components of attack response inhibition in fencing: Components of attack response inhibition in fencing

Abstract Applying the Go/No-Go paradigm to fencing, we investigated the relationship between the moment at which the No-Go signal appeared after a movement had been initiated and the time required by fencers to suppress the motor execution of a step-lunge. Secondarily, we determined a time threshold from which movement inhibition results in an error. The No-Go stimulus was represented by a real attack movement. 18 elite fencers and a fencing master were included in the study. Four force plates measured the horizontal components of the fencer's and master's reaction forces, which were used to calculate the time components of the attack and the response inhibition process. Also, the velocity and displacement of the master's and fencer's respective centres of mass were estimated using inverse dynamics. In all cases, cognitive inhibition processes were completed after the onset of movement. Movement time was calculated using four time components (muscle activation, muscle deactivation, transition and braking time). The results obtained revealed that cognitive processes were not significantly affected by the timing of the appearance of the No-Go signal. In contrast, movement time and its time components tended to decrease when the time delay between the No-Go stimulus and the onset of the fencer's movement increased. In conclusion, any attempt to withhold an attack movement when it has already started leads to an error that increases the risk of being hit by the opponent, especially when attack is inhibited within 150 ms after the movement has started.
Fungal Endophytic Communities of Two Wild Rosa Varieties With Different Powdery Mildew Susceptibilities

Powdery mildew (PM) is one of the most devastating and wide spread fungal diseases of rose, which seriously decrease its productivity and commercial value. In the present study, the endophytic fungal communities of two wild Rosa varieties (Rosa multiflora Thunb and R. multiflora var. carnea Redouté and Thory) with different PM susceptibilities were studied through Illumina MiSeq sequencer. A total of 14,000,424 raw reads were obtained from 60 samples, and 6,862,953 tags were produced after merging paired-end reads. 4462 distinct OTUs were generated at a 97% similarity level. It was found that only 34.2% of OTUs shared between two plant varieties. All of the OTUs were assigned into four fungal phyla, 17 classes, 43 orders, 86 families, 157 genera, and 208 species. Members of Ascomycota were found to be the most common fungal endophytes (EF) among all plant samples (93.7% relative abundance), followed by Basidiomycota (4.7% relative abundance), while Zygomycota and Glomeromycota were found to be rare and incidental. At each developmental stage of plants, the diversity and community structure of EF between two Rosa varieties showed significant differences. Both PCoA plots and PERMANOVA analyses indicated that developmental stage was the major factor contributing to the difference between the Rosa varieties (R2 = 0.348, p < 0.001). In addition, plant varieties and tissues were also important factors contributing to the difference (R2 = 0.031, p < 0.05; R2 = 0.029, p < 0.05). Moreover, Neofusicoccum, Rhodosporidium, and Podosphaera, etc., were found to be significantly different between two Rosa varieties, and some of the endophytes may play a role in PM resistance. These finding are encouraging to testify the potential use of these fungi in the biocontrol of PM in future studies.
A widespread thermodynamic effect, but maintenance of biological rates through space across life’s major domains

For over a century, temperature compensation (maintenance of biological rates with changing temperatures) has remained controversial. An alternative idea, that fitness is greater at higher temperatures (the thermodynamic effect), has gained increasing traction, and is being used to understand large-scale biodiversity responses to environmental change. Yet evidence in favour of each of these contrasting hypotheses continues to emerge. In consequence, the fundamental nature of organismal thermal responses and its implications remain unresolved. Here we investigate these ideas explicitly using a global dataset of 619 observations of four categories of organismal performance, spanning 14 phyla and 403 species. In agreement with both hypotheses, we show a positive relationship between the temperature of maximal performance rate (Topt) and environmental temperature (Tenv) for all traits. Next we demonstrate that relationships between Tenv and the temperature of maximal performance rate (Umax) are rarely significant and positive, as expected if a thermodynamic effect predominates. By contrast, a positive relationship between Topt and Umax is always present, but markedly weaker than theoretically predicted. These outcomes demonstrate that while some form of thermodynamic effect exists, ample scope is present for biochemical and physiological adaptation to thermal environments in the form of temperature compensation.
Comparative Proteomic Profiling of Tumor-Associated Proteins in Human Gastric Cancer Cells Treated with Pectolinarigenin

Pectolinarigenin (PEC), a natural flavonoid that is present in citrus fruits, has been reported to exhibit antitumor effects in several cancers. Though the mechanism of PEC-induced cytotoxicity effects has been documented, the proteomic changes that are associated with the cellular response to this flavonoid are poorly understood in gastric cancer cells. In this study, a comparative proteomic analysis was performed to identify proteins associated with PEC-induced cell death in two human gastric cancer cell lines: AGS and MKN-28. Two-dimensional gel electrophoresis (2-DE) revealed a total of 29 and 56 protein spots with significant alteration were screened in AGS and MKN-28 cells respectively. In total, 13 (AGS) and 39 (MKN28) proteins were successfully identified by mass spectrometry from the differential spots and they are known to be involved in signal transduction, apoptosis, transcription and translation, cell structural organization, and metabolism, as is consistent with multiple effects of PEC on tumor cells. Notably, novel target proteins like Probable ATP-dependent RNA helicase DDX4 (DDX4) and E3 ubiquitin-protein ligase LRSAM1 (LRSAM1) along with the commonly differential expressed proteins on both the cell lines that are treated with PEC were confirmed by immunoblotting. The DDX4 accelerates cell cycle progression by abrogating the G2 checkpoint when overexpressed in cancer cells, while the aberrant expression of LRSAM1 may be involved in the cancer pathology. Thus, proteomic analysis provides vital information about target proteins that are important for PEC-induced cell death in gastric cancer cells.
Spatial benefits afforded by high rank in white-faced capuchins

Group living is a source of both costs and benefits for animals. Benefits may include decreased predation risk, and an increased ability tofind food and defend clumped resources; the most prominent cost is probably increased competition for food within the group. Presumably, animals will always try to minimize the cost they receive relative to the corresponding benefit. Since costs and benefits will vary between spatial positions within the group, animals should prefer those spatial positions with the lowest costs relative to benefits. For groups whose members are organized by a social dominance hierarchy, access to preferred spatial positions may be a benefit of high rank. We examined the relationship between dominance rank and spatial patterns in two groups of white-faced capuchins,Cebus capucinus. We expected the animals to be faced with two cost-benefit gradients: predation risk increasing from centre to edge, and depletion costs increasing from front to back. Depletion was a significant factor in the dry season but not in the wet season; therefore, presumably only the predation risk gradient was present in the wet season. Dominant animals were more central than their subordinate counterparts during both seasons, and within the centre, they preferred the most forward position during the dry season but not during the wet season. The absence of variation in agonism across spatial positions suggests that active exclusion of subordinates by dominant animals cannot explain the spatial patterns observed. Instead, we conclude that subordinates avoid dominant animals as a strategy to reduce contest competition. 1997 The Association for the Study of Animal Behaviour
Extended song learning in wild European starlings

Abstract The songs of wild adult European starlings,Sturnus vulgaris, were recorded over successive years to determine whether repertoire size and composition changed between years. Repertoire-size estimates increased between the first and last year of recording for five of seven males that were at least 2 years old when first recorded. All seven birds showed extensive changes in the composition of their repertoires. Many phrase types were dropped from the repertoire, and others were modified. All birds added many new phrase types to their repertoires between years. These results indicate that extended song learning occurs in adult starlings, even in birds that are at least 4 years old, and that repertoire size is correlated with age in this species. The influence of song repertoire size on male-male competition and possibly female mate choice may be related to its value as an indicator of male age.
Towards a Simple Approach to Multi-step Model-based Reinforcement Learning

When environmental interaction is expensive, model-based reinforcement learning offers a solution by planning ahead and avoiding costly mistakes. Model-based agents typically learn a single-step transition model. In this paper, we propose a multi-step model that predicts the outcome of an action sequence with variable length. We show that this model is easy to learn, and that the model can make policy-conditional predictions. We report preliminary results that show a clear advantage for the multi-step model compared to its one-step counterpart.
A survey of public attitudes towards third-party reproduction in Japan in 2014

Objective: The objective of this study was to examine public attitudes towards third-party reproduction and the disclosure of conception through third-party reproduction. Methods: We conducted the web-based survey for the public attitude towards third-party reproduction in February 2014. Twenty-five hundred people were recruited with equal segregation of age (20s, 30s, 40s, and 50s) and gender. We analyzed the association between gender, age, infertility, and ethical view using a questionnaire regarding donor sperm, donor oocyte, donor embryo, gestational surrogacy, and disclosure to offspring. Results: Of the respondents, 36.2% approved and 26.6% disapproved with gamete or embryo donation. The frequency of those who approved was the lowest in females in the 50-59 years age group, and was significantly higher in males or females with infertility. Secondly, 40.9% approved and 21.8% disapproved with gestational surrogacy. The frequency of those who approved gestational surrogacy was higher in males or females with infertility. Thirdly, 46.3% of respondents agreed and 20.4% disagreed with “offspring have the right to know their origin”. Those who disagreed were primarily in the 50-59 age group of both genders, and disagreement was significantly higher in the infertility group compared with non-infertility group. Conclusion: In this study, public attitudes were affected by gender, age, and experience of infertility. These study findings are important in understanding the attitude towards third-party reproduction and disclosure to the offspring. Respondents having indecisive attitudes were >30%, which might indicate an increased requirement for information and education to enhance the discussion on the ethical consensus on third-party reproduction in Japan.
Male size determines reproductive output in a paternal mouthbrooding fish

Size can have strong effects on reproductive success in both males and females, and in many species large individuals are preferred as mates. To estimate the potential benefits from mate choice for size in both sexes, I studied the effects of the size of each sex on the reproductive output of pairs of Banggai cardinalfish, Pterapogon kauderni, a sexually monomorphic obligate paternal mouthbrooder. When pairs were allowed to form freely, a size-assortative mating pattern was observed and larger pairs had a higher reproductive output as determined by total clutch weight and egg size. To separate the potential benefits from mate choice for size for each sex, I subsequently used these pairs to form reversed size-assortative pairs, that is, the largest male paired to the smallest female and vice versa. I found a positive correlation between male size and clutch size: relatively heavier clutches were found in pairs where females were given a larger male. This suggests that the size of the male influences clutch weight. For egg size, however, the size of both sexes seemed important. The study reveals the benefits of mutual mate choice on size in this species: larger females provide larger eggs and larger males can brood heavier clutches. Furthermore, these results suggest that females differentially allocate resources into the eggs according to the size of the mate.
Reproductive competition and conflicts in colonies of the ant Formica sanguinea


Abstract Abstract. The ant Formica sanguinea is extremely suitable for examining reproductive competition among co-existing queens and queen-worker conflicts within colonies, because its colonies are often weakly polygynous and the queens are polyandrous. The mean relatedness, estimated by enzyme gene markers, was significantly lower among worker pupae (0·38) than among sexual female pupae (0·47) produced by the same nests. The most likely interpretation is that one of the co-existing queens dominates others in sexual production. The population sex ratio was female-biased (investment in males was 0·40), as predicted if sex allocation is controlled by workers. When the relatedness asymmetry varies between colonies (variation in the levels of polygyny and polyandry), worker control is expected to lead to split sex ratios provided that workers can assess the type of colony they are in. The distribution of colonial sex ratios was bimodal, but the colonial sex ratios were not associated with the relatedness asymmetry in the way predicted by the split sex-ratio hypothesis. This indicates that workers are unable to assess the relatedness asymmetry and/or to use that information for maximizing their inclusive fitness.
Primary Adrenal Insufficiency During Lenvatinib or Vandetanib and Improvement of Fatigue After Cortisone Acetate Therapy

Abstract Context Two tyrosine kinase inhibitors (TKIs), lenvatinib and vandetanib, are often used to treat advanced radioiodine-refractory differentiated thyroid cancer (RAI-R DTC) and medullary thyroid cancer (MTC), respectively. Fatigue is a common adverse event during treatment with these and other TKIs and a common cause of drug discontinuation or dosage reduction. Cases Description We evaluated the basal and stimulated adrenal function in 12 patients with advanced RAI-R DTC and MTC treated with lenvatinib or vandetanib, respectively. Ten patients complaining of fatigue showed a progressive ACTH increase with normal cortisol levels. Moreover, six of 10 patients had a blunted cortisol response after ACTH stimulation, thus confirming the diagnosis of primary adrenal insufficiency (PAI). The causal relationship between TKIs and PAI onset was also demonstrated by the repeated testing of adrenal function before and during treatment. Patients with PAI received cortisone acetate replacement therapy, with a substantial and prompt improvement in the degree of fatigue, as assessed by the Common Terminology Criteria for Adverse Events version 4.03, thus supporting the major impact of impaired adrenal function in the genesis of this adverse event. Conclusions We show that the occurrence of PAI may be a common cause of fatigue during lenvatinib and vandetanib treatment, and we therefore recommend testing adrenal function for a prompt start of replacement therapy to avoid treatment discontinuation, dosage reduction, and potentially severe PAI complications.
Neocortex size and social network size in primates

Primates use social grooming to service coalitions and it has been suggested that these directly affect the fitness of their members by allowing them to reduce the intrinsic costs associated with living in large groups. We tested two hypotheses about the size of grooming cliques that derive from this suggestion: (1) that grooming clique size should correlate with relative neocortex size and (2) that the size of grooming cliques should be proportional to the size of the groups they have to support. Both predictions were confirmed, although we show that, in respect of neocortex size, there are as many as four statistically distinct grades within the primates (including humans). Analysis of the patterns of grooming among males and females suggested that large primate social groups often consist of a set of smaller female subgroups (in some cases, matrilinearly based coalitions) that are linked by individual males. This may be because males insert themselves into the interstices between weakly bonded female subgroups rather than because they actually hold these subunits together.