Miracle-dz/UniFAR-FTUs huggingface dataset:
The Study of the Probiotic Potential of the Beneficial Bacteria Isolated from Kefir Grains

Abstract The aim of this study was to identify beneficial bacteria with probiotic potential from kefir grains. The lactobacilli isolated from kefir grains were characterised as: Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus paracasei, and Lactobacillus kefiri. The strains Lb. plantarum 1Ž, Lb. paraplantarum S10, and Lb. paracasei 2Ž tolerated better the test gastric juice at pH 2 and 2.6 during 120 min of incubation in comparison with the strains Lb. kefiri. On the other hand, the strains Lb. kefiri were resistant to 0.3 % bile acid salts. The Lb. paracasei 2Ž showed the significantly highest survival (P < 0.001) at pH 2 in comparison with all other strains tested and was also able to tolerate 0.3 % concentration of the bile salts. All strains produced medium to strong biofilms on abiotic surfaces and inhibited the growth of selected potential pathogens with varying intensity. All kefir isolates were susceptible to the antibiotics tested and exhibited positive β-galactosidase activity with the exception of Lb. paracasei 2Ž which did not show any activity of undesirable enzymes, such as β-glucosidase and β-glucuronidase. Additional testing and validation of the biological properties and safety of the strain Lb. paracasei 2Ž under in vivo conditions are needed to confirm the prospective use of this strain in practice.
An Alternative of Phosphopeptide Identification using Inclusion List-Driven Targeted Liquid-Tandem Mass Spectrometry for Label-Free Quantitative Tyrosine Phosphoproteomics: Application of Src Inhibitor-Related Signaling Pathway Analyses in Lung Cancer

Tyrosine phosphorylation plays a critical role in cell biological functions, particularly in signal transduction. Mass spectrometry (MS)-based proteomic analysis of tyrosine-phosphorylated (pTyr) proteins remains challenging due to their relatively low abundance. The methods of phosphopeptide enrichment are developed prosperously such as IMmuno Affinity Chromatography (IMAC) and Metal Oxide Affinity Chromatography (MOAC). However, the complexity of experimental design for sample separation induces the unnecessary variations, particularly in label-free study designs. Here, we report an alternative approach, Inclusion List-driven Targeted LCtandem MS (ILT LC-tandem MS), and demonstrate the results obtained are comparable with that of phosphopeptide enrichment for increasing the sequencing possibility of low abundant pTyr peptides. In the present study, ILT LC-M/MS was applied for comparative labelfree quantitative tyrosine phosphor proteomic analysis between Src inhibitor (dasatinib)-treated and untreated CL1-5 lung cancer cells. A total of 512 distinct pTyr sites corresponding to 491 peptides had been identified. Copyright: © 2014 AJRTGP. This is an open-access article distributed under the terms of the Creative Commons Attribution License, Version 3.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Volume 1 • Issue 1 • 103 www.aperito. org http://dx.doi. org/10.14437/AJRTGP-1-103 Page 2 of 19 Citation: Pao-Chi Liao (2014), An Alternative of Phosphopeptide Identification using Inclusion List-Driven Targeted Liquid-Tandem Mass Spectrometry for Label-Free Quantitative Tyrosine Phosphoproteomics: Application of Src Inhibitor-Related Signaling Pathway Analyses in Lung Cancer. Aperito J Recent Trends Genome Proteom 1:103
It takes time to see the menu from the body: an experiment on stable isotope composition in freshwater crayfishes

Key-words: nutrition, body composition, noble crayfish, signal crayfish, tissue anabolism For many applications and ecological studies in which wild individuals are brought to laboratory it would be essential to know accurately how fast novel diet is reflected in composition of different tissues. To study the effects of two different diets on the stable isotope composition of freshwater crayfish muscle and hemolymph, we conducted a three month experiment on noble crayfish (Astacus astacus) and signal crayfish (Pacifastacus leniusculus) by feeding them sweet corn (Zea mays) or Baltic herring (Clupea harengus membras) as novel food. During the experiment, the crayfish were given 0.4 g of selected food daily and the amount consumed was recorded. The samples for the stable isotope analyses were taken at the commencement of the experiment (initial control) and three times (hemolymph) or twice (muscle tissue) during the experiment. We found that stable isotope changes can be similarly, and rather slowly, detected from muscle tissue and hemolymph under studied conditions. Hemolymph sampling, being non-lethal, can be recommended as a practical sampling method. Our results confirm earlier reports according to which diet changes reflect to crayfish isotope ratios slowly implying that isotope ratios indicate long-term diet.
The Behavioural Consequences of Unfair Punishment

Experimental evidence from public good games with punishment suggests that punishment works when subjects assign it fairly by sanctioning non-cooperators. This paper reports an experiment in which punishment is assigned unfairly in the sense that it is not linked to individual behaviour and is melted out to all group members (irrespective of their prior behaviour). We test whether unfair punishment generates different contribution and punishment behaviour relative to the standard punishment game. Our findings suggest different dynamics of average contributions in the presence of unfair punishment relative to the standard punishment game. Contribution levels are significantly different only when subjects have obtained experience from both games. We also find that, although the assignment of punishment is unaffected after the experience of an environment with unfair punishment, a history of unfair punishment makes a difference regarding reactions to alleviation, reward and punishment received.
Local Economic Development-Based on Farming Manufacture of Coconut Sugar in West Sei Kepayang District

The existence of coconut commodity abundance in the district of West Sei Kepayang of Asahan regency of North Sumatra province has not been utilized to the fullest. Though the potential of this commodity can be the basis of local economic development (LED). Of some its derivative products, coconut sugar has the economic potential to improve farmers welfare due to various advantages as well as a good market share prospect.This study aims to analyze the potential for the farm manufacture of coconut sugar to be used as a base of LED in the district of West Sei Kepayang. Data collected by survey method and then analyzed by descriptive quantitative by a financial feasibility analysis those are the Net Present Value (NPV), Net Benefit Cost Ratio (NBCR), Internal Rate of Return (IRR) and Payback of Period (PP) and by descriptive qualitative. The results of a financial feasibility analysis showed that the NPV is positive, NBCR is greater than one, the IRR is greater than the actual interest rate and PP can be restored in a relatively short time. Keywords : Local Economic Development, Coconut Sugar, Financial Feasibility Analysis.
Morphological analysis of leaf and tiller number dynamics of wheat (Triticum aestivum L.): responses to temperature and light intensity

In recent literature on Gramineae species, leaf and tiller number dynamics have been studied by analysing site filling and the phyllochron of the mainstem. However, site filling is influenced by three components: (1) the phyllochron of the mainstem and daughter tillers; (2) specific site usage (i.e. fraction of buds that ultimately develop into a visible tiller at a specific site); and (3) HS-delay (i.e. difference in Haun Stage (HS) between the parent tiller and daughter tiller above the point where the daughter tiller appears). These three morphological components affecting site filling were studied under different environmental conditions in a growth chamber experiment with spring and winter wheat (Triticum aestivumL.). Treatments were temperature (daily average 10.5, 15.5 or 20.5 °C) and light intensity (111, 191 or 286 μmol m−2s−1). Effects of temperature and light intensity on phyllochron were well described by equations already reported in the literature. Specific site usage was higher at cooler temperatures and greater light intensities and was related to tiller position. It is proposed that these effects on specific site usage reflect differences in availability of local assimilate for tiller appearance. HS-delay of a tiller was shorter if the expected tiller appearance was later and was only slightly affected by light intensity or temperature. This new concept, combining HS-delay and specific site usage, can be useful in constructing more general models of the effects of environmental factors on the dynamics of leaf number and leaf area ofGramineaespecies.
Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters

Acute myeloid leukemia (AML) is a hematological malignancy of myeloid progenitor cells characterized by anomalous proliferation, inhibition of differentiation and expansion of leukemic cells blocked at the early stage of hematopoiesis. The molecular markers have become a smart tool to further division of AML patients into subgroups. Nucleophosmin (NPM1) mutations are found in approximately 30% of adult AML patients and are associated with a favorable outcome when detected in absence of FLT3-ITD mutation. Over 50 molecular NPM1 mutation variants have been identified; the most common one is NPM1-A mutation. The current study aims to detect; the frequency of NPM1-A in Iraqi adult newly diagnosed AML patients using real time – PCR technique and evaluate the relationship of NPM1-A with age, gender, total white blood cell (WBC) count and FAB subtypes of the disease. The frequency of NPM1-A mutation in newly diagnosed AML patients was 18.86%. Age was non-significantly higher in NPM1-A mutated patients than in patients without mutation (P=0.538). The NPM1-A mutation was predominantly seen in male (P= 0.069). The mutation was non-significantly higher among monocytic subtypes (M5+M4) compared with the other subtypes of the disease (P=0.916). In the NPM1 mutated group, the total WBC count was non-significantly higher than the non-mutated group (P=0.302). These findings suggest; Real time-PCR technique using TaqMan probe was very specific molecular test for this mutation. Mutated NPM1-A patients were associated with increased age, a higher total WBC count, male predominance and the monocytic subtypes of the disease.
Framing Differences Between Local and National Media in the Trayvon Martin Case

The purpose of this study was to explore the difference in media representations in local and national news by examining the 2012 Trayvon Martin case between the parameters of February 26, 2012April 12, 2012. In this case, an African American adolescent was shot and killed in Sanford, Florida, by a neighborhood watch coordinator by the name of George Zimmerman. Through a mix method approach, the research used content and discourse analysis as tools to analyze and gather data from the New York Times and Orlando Sentinel in the coverage of the case. Content analysis was used to give an overview of the data set, while discourse analysis helped describe the interpreted meanings that were constructed within the articles’ text to shed light on media framing. To further examine the research question, the study also analyzed if there was a difference in the Orlando Sentinel’s coverage before and after Trayvon Martin case became national news. The findings discovered that the New York Times emphasizes race more than the Orlando Sentinel. Further analysis showed that once the New York Times picked up the coverage on the Trayvon Martin case on March 16, 2012, there was a difference in the Orlando Sentinel’s headlines and language used within their article. Additionally, the findings revealed that the news used its ‘power’ to subtly reinforce and maintain longstanding racialized stereotypes of Blacks with the use of media framing. The Trayvon Martin case gave me the opportunity to examine the complexity and dynamics of the creation of racialized stereotypes and how the ‘powerful’ role news plays in reinforcing, perpetuating, and creating discourses.
An Exploration into the Impact of the Resettlement Experience, Traditional Health Beliefs and Customs on Mental Ill‐Health and Suicide Rates in the Ethiopian Community in London

Forced migration and the resettlement experience combine to produce a set of social, cultural, economic and psychological challenges for forced migrants which may affect integration, mental and physical health, and access to health and social care. There is very little research on the resettlement experience of Ethiopian forced migrants in London, particularly on causes of mental illness and access to mental health care. Few studies have examined whether and how traditional beliefs and customs affect the experiences of this group in health care. The paper reports on a pilot study consisting of interviews with an Ethiopian priest, community leaders and Ethiopians working in the community health sector with the aims of improving our understanding of the issues, and to inform further study. Initial analysis suggests that this group faces multiple forms of disadvantage which affect mental health. A further interesting dynamic is the relation between lack of ‘help seeking behaviour’, due to cultural expectations and norms, and lack of access and engagement with Western treatments. Religious mechanisms and activities were also reported as bolstering coping mechanisms. Perhaps most significant was concern about the increasing suicide rate among this group, many respondents suggesting a direct causal link between suicide and maladjustment in exile.
The wellbeing of childless men and fathers in mid-life

ABSTRACT Using data from the first wave of the Netherlands Kinship Panel Study conducted in 2002–03, this paper examines the economic, psychological and social wellbeing among 1,467 men aged 40–59 years with different parenthood histories and circumstances: the childless, fathers who live with their children, non-co-resident fathers, and ‘empty-nest fathers’. The gerontological interest is whether there are variations in wellbeing by parenting, and whether they persist in old age. The results showed that fathers have higher incomes than childless men, regardless of their partner history. As regards psychological wellbeing, men's partner history counts, not their parenthood status. Being single contributes to low levels of psychological wellbeing. The findings provide evidence of the socially integrating effects of parenthood and for men's ‘good-provider’ role. Childless men and non-co-resident fathers report poorer quality family relationships. In addition, childless men were least likely to report helping others in the community. Overall, more support is found for the notion that fatherhood is a transforming event than that the wellbeing benefits derive from fathering activities. The paper concludes with a discussion of the implications of the findings for inequalities in wellbeing and informal support among the male members of the cohort born during 1943–63 when they reach old age.
Predictors of intention to leave of public secondary school teachers in Tanzania: Exploring the impact of demographic factors, intrinsic and extrinsic satisfaction

This study reports part of a larger survey that was conducted in three public secondary schools in Dodoma region, Tanzania. It was agreed by researchers and participating schools that school names will not appear in any publications but abbreviations (S1, S2 and S3). The purpose of this paper was to investigate the relationship and impact of job satisfaction dimensions (intrinsic and extrinsic satisfaction) on intention to leave among teachers in public secondary schools. Further, it explored levels of job satisfaction, intention to leave and whether demographic characteristics had impact on teachers' intention to leave level. Respondents were randomly selected through "instant invite and questionnaire take" technique and given self-administered questionnaire to fill. Statistical techniques (Correlation, Regression, t-test and ANOVA) were used to analyze and interpret results. Results indicated moderate general satisfaction, intrinsic satisfaction and extrinsic satisfaction meanwhile it showed high intention to leave in teachers. Results also revealed intrinsic and extrinsic satisfaction had significant negative relationship with intention to leave and intrinsic satisfaction indicated stronger prediction of teachers' intention to leave. While gender and location did not show differences in intention to leave mean scores, education and age revealed significant differences. Implications for responsible officials/agencies and strategies for improvement were discussed. Keywords: Demographic characteristics, Extrinsic satisfaction, Intrinsic satisfaction, Intention to leave, Job satisfaction, Tanzania
Acoustic Analysis of Machineries in the Cement Industry

A study has been conducted at the Tehran cement factory to recognize and analyze the major noise emission source machineries. Cement industry has many process units. Basically all of these units or stages can be considered as a source of noise. Since noise pollution is defined based on its offensive hearing effects, the importance of the noise sources depends directly on the number of workers in the unit. In this survey, first, the mean A-weighted sound pressure level and number of the workers in each unit have been measured and the obtained data were analyzed in Microsoft Excel. The noisiest units have been recognized and some applicable suggestions offered to reduce the sound exposure level. Afterwards, based on the mentioned ISO, some experiments have been done to calculate the A-weighted acoustic power level of the crusher which emitted the highest level of noise. By calculating the sound power levels and considering the affecting design parameters on the sound power generated by the crusher, an empirical equation has been presented to calculate the acoustic power level as a function of physical parameters horsepower, number of hammers and the weight of each hammer.
Review and guide to a future naming system of African Bemisia tabaci species

Once a pest has been correctly identified, its genus and species name can provide a link to valuable indications of its ecology, biology and life history that are critical for developing control strategies. Importantly, this link should exist even when the pest was known under other names (synonyms), or was not considered a pest at all (National Research Council, 1968). Many examples have shown that incorrect identification or classification of a pest has led to fruitless searches for biocontrol agents in the native range, incorrect assignments as disease vectors, and costly, yet misdirected, suppression measures. As new approaches for delimiting species based on molecular information become more widely used, the process of correctly identifying a species has become even more complex. Fortunately, we have good systematic frameworks and nomenclatural systems that are able to cope with these challenges. Here we review challenges associated with classification and identification within the Bemisia tabaci (Gennadius) species complex. These pests and the viruses they transmit have emerged in the past few decades as among the most damaging to food and fibre crops globally (Varma & Malathi, 2003; Pimental et al., 2005; Seal et al., 2006), especially in sub‐Saharan Africa (SSA). The systematics of the B. tabaci species group has been a highly debated topic for years (Boykin, 2014). Putative species are indistinguishable morphologically, so other biological data have been collected to investigate the species in the complex. Based on genetic differences (Colvin et al., 2004; Sseruwagi et al., 2005; Boykin et al., 2007; Boykin et al., 2013; Hsieh et al., 2014) and mating incompatibility (Colvin et al., 2004; Liu et al., 2007; Xu et al., 2010), B. tabaci is now recognized as a species complex that consists of at least 34 putative species (Boykin et al., 2012). The rapid discovery of significant species diversity has led to many changes in the informal names used over the last 10 years (Boykin, 2014), creating confusion in the literature.
Randomization-based models for multitiered experiments: I. A chain of randomizations

We derive randomization-based models for experiments with a chain of randomizations. Estimation theory for these models leads to formulae for the estimators of treatment effects, their standard errors and expected mean squares in the analysis of variance. We discuss the practicalities in ﬁtting these models and outline the difﬁculties that can occur, many of which do not arise in two-tiered experiments.
Experimental behavior of circular foundations on oil contaminated sand

This paper studies the behavior of circular footings placed on the gasoil and kerosene-contaminated soil. The ultimate objective of this study is to determine the effect that oil contamination has on the sand bearing capacity. The contaminated sand layers were mixed with different levels of between 1 and 4 percent of gasoil and kerosene contamination. The influence of the contamination depth and type of contamination was examined in this study. Laboratory tests were conducted on small scale models and performed in load-controlled manner. The results showed that the contamination can influence the load-settlement curves and lead to a reduction in its load-bearing capacity. An increase in the depth and percentage of contamination decreased the bearing capacity. Some formulas have been provided in which by knowing the depth and percentage of contamination one can predict the third bearing capacity factor of a circular foundation
Improvement of silver nanoparticle impregnation on cotton fabrics using a binder

In this work, silver nanoparticles were synthesized by the polyol process, which reduces silver nitrate with ethylene glycol. The temperature effect and the polyvinylpyrrolidone (PVP) amount were studied. The temperatures used were 100, 120 and 140 °C. Three ratios of 0.00, 0.25, and 0.50 (% w/w) of PVP/AgNO 3 were established. Nanoparticles with sizes less than 30 nm were obtained in conditions of 120 °C and 0.5 % w/w of PVP/AgNO 3 . Posteriorly, nanoparticles in concentrations of 10 and 20 ppm were deposited on cotton fabric by the pad-dry-cure technique, in order to analyze their bactericidal properties against the Gram positive bacteria Staphylococcus aureus 25923. The tests showed that cotton fabric with nanoparticles in concentrations of 10 and 20 ppm had good bactericidal properties, reducing bacterial colonies by over 98 %. Finally, a washing stability study of the fabrics impregnated with silver nanoparticles was performed, and an acrylic binder was added during the impregnation process. The results were obtained by bacteriological analyzes.