Draft genome sequence of an enterococcus faecalis strain ( 24 FS )

Enterococcus faecalis 24FS is a bacteriocin-producing, multiply antibioticresistant, and potentially virulent bacterium isolated from healthy infant feces. The draft 2.9-Mb genome sequence revealed 2,968 protein-encoding genes; 11 antibiotic resistance, 8 virulence, and 3 bacteriocin genes; and 2 plasmids, 4 prophages, 30 insertion sequence (IS) elements, 1 transposon, and 1 integron. Enterococcus faecalis is a common commensal of the human intestine (1, 2) but is also a frequent cause of hospital-acquired enterococcal infections (3). An E. faecalis fecal isolate (24FS) from a healthy 6-month-old infant (Alexandria, Egypt) displayed multipleantibiotic resistance, strong antibacterial properties, and numerous virulence factors and thus provided the opportunity to study the emergence of pathogenic/resistance properties in a nonclinical strain from a developing nation. Strain 24FS was isolated from infant feces (in 2016) on bile esculin azide agar. Genomic DNA was isolated using a GeneJET purification kit. Genome sequencing was performed by MicrobesNG (Birmingham, UK) on MiSeq and HiSeq 2500 platforms (Illumina) with 30 sequence coverage, giving 924,992 reads with a median insert size of 529 bases and a 136-fold coverage of the genome. The reads were trimmed using Trimmomatic (4) (v 0.38) by identification of adapter sequences, and the quality of trimmed reads was assessed using in-house scripts combined with the BWA-MEM software (5) (v 0.7.9). De novo assembly was performed using SPAdes (6) (v 3.7.0), yielding 46 contigs of more than 1,000 bp. Assembly quality was assessed using QUAST (7). The 2,984,798-bp draft genome has 2,968 protein-encoding genes, 54 tRNA genes, and 111 pseudogenes and a G C content of 37.38%, as predicted with Rapid Annotations using Subsystem Technology (RAST) (8, 9). E. faecalis 24FS showed a broad-spectrum antibacterial activity (in spot on the lawn assays [10]), inhibiting the pathogens Listeria monocytogenes 10403S, Salmonella enterica PT4, Staphylococcus aureus, and Pseudomonas aeruginosa PAO1. The PATtyFam algorithm identified three bacteriocin genes encoding a colicin V (20,317 Da), the class IIa bacteriocin hiracin-JM79 (7,737 Da), and an enterocin A (13,264 Da). Strain 24FS is -hemolytic (11), which is consistent with the observed presence of yqfA, encoding hemolysin III. It also has high adherence and biofilm-formation capacity (12), correlating with the presence of virulence-related adherence/biofilm genes, namely, gelE (gelatinase), esp (gene encoding a surface protein), ace (collagen adhesin), cvfB (conserved virulence factor B), and ebpABC (pili-encoding genes). The disk diffusion method (13) showed that the strain is resistant to chloramphenicol, tetracycline, and erythromycin but sensitive to penicillin and streptomycin, which is consistent with the presence of corresponding resistance genes (cat, chloramphenicol; tetA, tetC, tetL, tetR, and ykkCD, Citation El Halfawy NM, El-Naggar MY, Andrews SC. 2019. Draft genome sequence of an Enterococcus faecalis strain (24FS) that was isolated from healthy infant feces and exhibits high antibacterial activity, multiple-antibiotic resistance, and multiple virulence factors. Microbiol Resour Announc 8:e00047-19. https://doi.org/10.1128/MRA.00047-19. Editor Jason E. Stajich, University of California, Riverside Copyright © 2019 El Halfawy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Simon C. Andrews, s.c. andrews@reading.ac. uk. Received 11 January 2019 Accepted 27 February 2019 Published 28 March 2019 GENOME SEQUENCES