Miracle-dz/UniFAR-FTUs huggingface dataset:
Isolation and characterization of a Lactobacillus amylovorus mutant depleted in conjugated bile salt hydrolase activity: relation between activity and bile salt resistance

Growth experiments were conducted on Lactobacillus amylovorus DN‐112 053 in batch culture, with or without pH regulation. Conjugated bile salt hydrolase (CBSH) activity was examined as a function of culture growth. The CBSH activity increased during growth but its course depended on bile salts type and culture conditions. A Lact. amylovorus mutant was isolated from the wild‐type strain of Lact. amylovorus DN‐112 053 after mutagenesis with N‐methyl‐N′‐nitro‐N‐nitrosoguanidine. An agar plate assay was used to detect mutants without CBSH activity. In resting cell experiments, the strain showed reduced activity. Differences between growth parameters determined for wild‐type and mutant strains were not detected. Comparative native gel electrophoresis followed by CBSH activity staining demonstrated the loss of proteins harbouring this activity in the mutant. Four protein bands corresponding to CBSH were observed in the wild‐type strain but only one was detected in the mutant. The specific growth rate of the mutant strain was affected more by bile salts than the wild‐type strain. Nevertheless, bile was more toxic for the wild‐type strain. In viability studies in the presence of nutrients, it was demonstrated that glycodeoxycholic acid exerted a higher toxicity than taurodeoxycholic acid in a pH‐dependent manner. No difference was apparent between the two strains. In the absence of nutrients, the wild‐type strain died after 2 h whereas no effect was observed for the mutant. The de‐energization experiments performed using the ionophores nigericin and valinomycin suggested that the chemical potential of protons (ZΔpH) was involved in Lactobacillus bile salt resistance.
Clinical and cognitive diversity of psychotic states arising in late life (late paraphrenia)

SYNOPSIS This study aimed to explore the heterogeneity of paranoid psychosis with onset in late life by using cognitive factors in a centroid method of cluster analysis. Forty-seven subjects were allocated to two different clusters, the first with 24 (51·1 %) and the second with 23 (48·9 %) patients. Their cognitive attainment was evaluated against the performance of 33 elderly controls, all groups being matched for age, sex, and the numbers of years of education. Patients in cluster 2 showed a pattern of widespread cognitive impairment, which involved general measures of cognitive performance (MMSE, CAMCOG, WAIS-R verbal and performance scores), memory (digit and spatial span, delayed matching-to-sample, recognition memory for words and faces), and executive functions (verbal fluency, extra and intra-dimensional shift ability, spatial working memory, and planning). In contrast, patients in cluster 1 were only impaired on their extra-dimensional set shift and planning abilities, suggesting a more specific and restricted executive functioning deficit. We also analysed the impact that the use of antipsychotic medication could have had on patients' cognitive performance, which was shown to be negligible. In addition, there was no difference between the clusters with regard to the number of patients using neuroleptics, suggesting that the medication was unlikely to have introduced a performance bias in the two patient clusters. The validity of the subdivision of these patients into two separate groups was further supported by other clinical findings. Patients in cluster 1 exhibited more severe psychotic symptoms, as measured by the SAPS, than their counterparts in cluster 2, and were also more likely to display first-rank symptoms of Schneider. Conversely, cluster 2 membership was strongly associated with the presence of neurological signs and negative symptoms. We suggest that psychotic states arising in late life are a heterogeneous condition that may be best divided in two: ‘type A’, including patients with a wide range of psychotic symptoms, mild increase in the frequency of neurological signs, and cognitive deficits restricted to executive functions, and ‘type B’, which includes patients with less complex psychotic symptoms associated with a marked increase in the frequency of neurological signs and generalized cognitive impairment. The basis for this subdivision and the prospect for future studies are discussed.
One session of partial‐body cryotherapy (−110 °C) improves muscle damage recovery

To evaluate the effects of a single session of partial‐body cryotherapy (PBC) on muscle recovery, 26 young men performed a muscle‐damaging protocol that consisted of five sets of 20 drop jumps with 2‐min rest intervals between sets. After the exercise, the PBC group (n = 13) was exposed to 3 min of PBC at −110 °C, and the control group (n = 13) was exposed to 3 min at 21 °C. Anterior thigh muscle thickness, isometric peak torque, and muscle soreness of knee extensors were measured pre, post, 24, 48, 72, and 96 h following exercise. Peak torque did not return to baseline in control group (P < 0.05), whereas the PBC group recovered peak torques 96 h post exercise (P > 0.05). Peak torque was also higher after PBC at 72 and 96 h compared with control group (P < 0.05). Muscle thickness increased after 24 h in the control group (P < 0.05) and was significantly higher compared with the PBC group at 24 and 96 h (P < 0.05). Muscle soreness returned to baseline for the PBC group at 72 h compared with 96 h for controls. These results indicate that PBC after strenuous exercise may enhance recovery from muscle damage.
Municipal land allocations: a key for understanding tenure and social mix patterns in Stockholm

ABSTRACT A socially mixed population is a political ambition in Stockholm. By providing a mix of tenure alternatives throughout all neighbourhoods this objective could, at least partially, be fulfilled. Since current tenure proportions display a weak balance in many neighbourhoods it could be assumed that governing politicians – by primarily utilizing Stockholm’s vast landownership and municipal housing developers – attempt to bridge observed gaps. Distribution of new rental and ownership apartments in municipal land allocations should acknowledge the existing tenure composition in a neighbourhood. Methodically this article focuses on all (nearly 50,000) apartments channelled through Stockholm’s land allocation system between 2002 and 2012. After classification of all apartments based on tenure, location, year and developer (private or municipal) the information is merged with yearly housing stock characteristics for 128 neighbourhoods. The outcome is a unique data set allowing for statistical assessment of whether Stockholm’s tenure (and in extension social) mix ambition is reflected in practice. The present article aims to highlight the crucial importance of landownership in Swedish municipalities with an aspiration to achieve or maintain a balanced tenure mix. While the findings indicate Stockholm is complying fairly well with its ambition, the results do reveal some contradicting signs.
Mammary Glands of Aly Mice: Developmental Changes and Lactation‐Related Expression of Specific Proteins, α‐Casein, GlyCAM‐1 and MAdCAM‐1

PROBLEM: (1) Aly mouse is an autosomal recessive mutant mouse that lacks lymph nodes and Peyer's patches. Defects of mammary glands might explain why these mice fail to nurture their pups. (2) Expressions of glycosylation‐dependent cell adhesion molecule 1 (GlyCAM‐1) and mucosal addressin cell adhesion molecule‐1 (MAdCAM‐1) in mammary glands of lactating mice have been suggested, but only scant information is available. METHODS: The thickness and weight of mammary glands were measured directly. The expression of specific proteins, α‐casein, GlyCAM‐1, and MAdCAM‐1 in mammary glands was detected by reverse transcription‐polymerase chain reaction (RT‐PCR). MAdCAM‐1 expression was also confirmed by immunohistochemistry (IHC). RESULTS: Fat pads of mammary glands of homozygous (aly/aly) mice were small and weighed less than those of heterozygous (aly/+) mice. An increase in end‐bud development was evident in aly/aly mice. During pregnancy, growth of the mammary gland and alveolar formation were noted in both groups. The expression of α‐casein, GlyCAM‐1 and MAdCAM‐1 was detected in both groups of mice by RT‐PCR from late‐pregnancy. Immunohistochemically, MAdCAM‐1 was noted in small vessels around the mammary globules. CONCLUSIONS: Our results demonstrated marked changes in the structure of the mammary glands of aly/aly mice. The expression of lactation‐related proteins was detected during development, pregnancy, and lactation in both aly/aly and aly/+ mice.
Effect of Enthesitis on 1505 Brazilian Patients with Spondyloarthritis

Objective. To analyze the clinical effect of enthesitis in a large Brazilian cohort of patients with spondyloarthritis (SpA). Methods. A common protocol of investigation was prospectively applied to 1505 patients with SpA in 29 centers in Brazil. Clinical and demographic variables and disease indexes were investigated. The Maastricht Ankylosing Spondylitis Enthesitis Score was used to investigate the enthesitis component. Ankylosing spondylitis was the most frequent disease in the group (65.4%). Others were psoriatic arthritis (18.4%), undifferentiated SpA (6.7%), reactive arthritis (3.3%), and enteropathic arthritis (3.2%). Results. At least 1 affected enthesis was observed in 54% of the patients with SpA, with a mean of 2.12 ± 2.98 entheses affected. According to the clinical presentation, enthesitis was significantly more frequent in patients with axial + peripheral joint involvement compared to isolated axial or peripheral involvement (p < 0.001). There was a statistical association between the presence of enthesites and axial symptoms (buttock pain, cervical pain, and hip pain), and peripheral symptoms (lower limb arthritis, number of painful and swollen joints; p < 0.05). Patients with enthesitis also presented higher mean scores of Bath Ankylosing Spondylitis Functional Index (BASFI; p < 0.001), Bath Ankylosing Spondylitis Disease Activity Index (p < 0.001), and Ankylosing Spondylitis Quality of Life (ASQoL; p < 0.001). Multivariate logistic regression showed that BASFI (p < 0.0001; OR 74.839), ASQoL (p = 0.0001; OR 14.645), and Achilles tendonitis (p = 0.0059; OR 7.593) were associated with work incapacity. Conclusion. The clinical presence of enthesitis in this large cohort of patients with SpA was frequent and was associated with a significant increase in disease activity and decline in functional capacity and quality of life.
Neuroscience and Biobehavioral Reviews Emotion regulation: Quantitative meta-analysis of functional activation and deactivation involve distinct subsets of frontocortical structures.

Emotion regulation is hypothesized to be a multifaceted process by which individuals willfully modulate the intensity and direction of emotional response via prefrontally mediated inhibition of subcortical response-related regions of the brain. Here we employ activation likelihood estimation (ALE) meta- analysis of functional magnetic resonance imaging studies to (1) reveal a consistent network of structures active during emotion regulation, (2) identify the target regions inactivated by the willful regulation pro- cess, and (3) investigate the consistency of activated structures associated with downregulation and upregulation. Results reveal signal change in bilateral amygdala/parahippocampal gyrus that decreased in downregulated states and increased in upregulated states, while cortical regions including superior frontal gyrus, cingulate, and premotor areas exhibited enhanced activity across all regulation conditions. These results provide consistent evidence for the role of amygdala activity in experienced emotional intensity, where intentional dampening and exaggeration are clearly expressed. However, the execution of emotional upregulation and downregulation may
The clinical effect of glucocorticoids in patients with rheumatoid arthritis may be masked by decreased use of additional therapies.

OBJECTIVE Our previous analysis of patients with early active rheumatoid arthritis (RA) treated with prednisone or placebo revealed the following discrepancy: although a significant retardation of joint damage was observed in the prednisone group compared with the placebo group, no differences in clinical variables between the 2 groups were observed, due to greater use of additional therapy in the placebo group. We sought to investigate whether this discrepancy would extend to variables of well-being. METHODS We conducted a double-blind, randomized, placebo-controlled clinical trial of prednisone (10 mg) in patients with RA; the duration of the study was 2 years. Following the placebo-controlled trial, a 1-year open-label followup study was conducted in 81 patients with early (</=1 year) active, previously untreated RA. Forty-one patients were allocated to receive oral prednisone, 10 mg/day, and 40 patients were assigned to the placebo group. Analgesics, nonsteroidal antiinflammatory drugs (NSAIDs), local injections of a glucocorticoid (only when absolutely necessary), and use of physiotherapy were allowed in both groups. After 6 months, sulfasalazine (2 gm/day) could be prescribed as rescue therapy in both groups. At the beginning of the study and every 6 months thereafter, 2 questionnaires (the VDF [Dutch version of the Health Assessment Questionnaire] and the IRGL [Dutch version of the Arthritis Impact Measurement Scales]) were administered. A visual analog scale (VAS) for morning pain was administered every 3 months. Disease activity and radiologic scores were assessed. RESULTS VDF scores in the 2 groups were not statistically significantly different. No statistically significant differences between groups were observed in almost all parameters of the IRGL. In the prednisone group (and only at 6 months), the VAS scores for morning pain and general well-being showed improvement comparable with the transient improvement in some of the disease activity variables. In the prednisone group, use of NSAIDs, analgesics, local injections of glucocorticoids, and physiotherapy sessions was approximately 50% that in the placebo group. CONCLUSION Although significant retardation of joint damage in the prednisone group indicates better disease control, no differences between the groups for variables of well-being were found. This discrepancy may be attributed to greater use of additional therapy in the placebo group. In future clinical trials, the use of additional therapies should be taken into account when analyzing the differences in effect between drugs.
Centralization of strategic decisions during the Great Recession

This study analyzes which types of firm - level shocks were associated with the centralization of strategic decision - making during the recession of 2008 - 09. We use a unique survey dataset of more than 14000 manufacturing firms from seven European countries which includes direct information on whether the firms centralized or decentralized their strategic decision - making process. Motivated by theoretical approaches claiming that organizations under considerable stress are more likely to centralize, we use multinomial logit models to test whether firms facing a larger fall in turnover, employment, investment or having to postpone their innovations were more likely to change their decision - making process. We find evidence that employment change and po stponing innovations are indeed associated with centralization even when we control for ownership, group structure, financing, management, and strategy.
Alternative and rescue treatment regimens for Helicobacter pylori eradication

Eradication therapy has been incorporated into clinical practice. The regimens currently recommended for first-line treatment include a 2-week bismuth-based triple therapy (mainly in developing countries), a 1 – 2 week proton pump inhibitor (PPI)-based triple therapy and a 1-week ranitidine bismuth citrate (RBC)-based triple therapy. However, these regimens fail to eradicate Helicobacter pylori in up to 20% of patients due to poor compliance, inadequate treatment duration, smoking, old age and bacterial resistance to nitroimidazoles and/or macrolides in particular. Therefore, alternative regimens that avoid nitroimidazoles and/or macrolides or overcome bacterial resistance to these drugs, improve compliance, minimise side effects and/or reduce costs have been evaluated. One-week quadruple therapy, which adds a PPI or histamine receptor 2-blocker to bismuth-based triple therapy, usually achieves an eradication rate of 90% when used as an alternative first-line therapy but the efficacy decreases when used as a rescue therapy. Several new triple therapies that may be used as alternative and/or rescue therapies have been evaluated. Among these are furazolidone-based (furazolidone plus an antibiotic and a bismuth salt, a PPI or RBC), fluoroquinolone-based (levofloxacin or moxifloxacin plus an antibiotic and a PPI) and ecabet sodium-based (ecabet plus two antibiotics) triple therapies. Recently, rifabutin has been used in combination with a PPI and amoxycillin as a rescue therapy, with satisfactory eradication rates. In addition, a number of new antimicrobial agents are currently under investigation in in vitro studies but the clinical values of these agents needs to be confirmed.
Trust: A phenomenological study of leaders and volunteers in LDS Seminaries and Institutes

The researcher conducted a qualitative student to develop a deeper understanding of how volunteers and leaders in the Seminaries and Institute (S&I) program of The Church of Jesus Christ of Latter-day Saints perceived trust between one another. Participants were coordinators and volunteer seminary and institute instructors from the US Southern Plains area of S&I. Data were collected from participant interviews and survey responses. The researcher identified three overarching categories as relevant to volunteer perception of trust in coordinators: effective teaching, helpful and discouraging training, and genuine or self-serving administrating. Further the researcher identified three overarching categories as relevant in recognizing the way coordinators perceived they were trusted by volunteers: superior teaching, implementation of coordinator instruction, and maintaining relationships. The findings of this study have implications for coordinators and volunteers who labor within the S&I organization. One glaring finding of this study was that the antecedents of trust, namely ability, benevolence, and integrity, were more readily
Cox Models With Smooth Functional Effect of Covariates Measured With Error

We propose, develop, and implement a fully Bayesian inferential approach for the Cox model when the log hazard function contains unknown smooth functions of the variables measured with error. Our approach is to model nonparametrically both the log-baseline hazard and the smooth components of the log-hazard functions using low-rank penalized splines. Careful implementation of the Bayesian inferential machinery is shown to produce remarkably better results than the naive approach. Our methodology was motivated by and applied to the study of progression time to chronic kidney disease as a function of baseline kidney function and applied to the Atherosclerosis Risk in Communities study, a large epidemiological cohort study. This article has supplementary material online.
Genetic Analysis of Dioxin Dioxygenase ofSphingomonas sp. Strain RW1: Catabolic Genes Dispersed on the Genome

ABSTRACT The dioxin dioxygenase of Sphingomonas sp. strain RW1 activates dibenzo-p-dioxin and dibenzofuran for further metabolism by introducing two atoms of oxygen at a pair of vicinal carbon atoms, one of which is involved in one of the bridges between the two aromatic rings, i.e., an angular dioxygenation. ThedxnA1 and dxnA2 cistrons encoding this dioxygenase have been cloned and shown to be located just upstream of a hydrolase gene which specifies an enzyme involved in the subsequent step of the dibenzofuran biodegradative pathway. Genes encoding the electron supply system of the dioxygenase are not clustered with the dioxygenase gene but rather are located on two other distinct and separate genome segments. Moreover, whereas expression ofdxnA1A2 is modulated according to the available carbon source, expression of the dbfB gene encoding the ring cleavage enzyme of the dibenzofuran pathway, which is located in the neighborhood of dxnA1A2 but oriented in the opposite direction, is constitutive. The scattering of genes for the component proteins of dioxin dioxygenase system around the genome ofSphingomonas sp. strain RW1, and the differential expression of dioxin pathway genes, is unusual and contrasts with the typical genetic organization of catabolic pathways where component cistrons tend to be clustered in multicistronic transcriptional units. The sequences of the α and β subunits of the dioxin dioxygenase exhibit only weak similarity to other three component dioxygenases, but some motifs such as the Fe(II) binding site and the [2Fe-2S] cluster ligands are conserved. Dioxin dioxygenase activity in Escherichia coli cells containing the cloned dxnA1A2 gene was achieved only through coexpression of the cognate electron supply system from RW1. Under these conditions, exclusively angular dioxygenation of dibenzofuran and dibenzo-p-dioxin was obtained. The dioxin dioxygenase was not active in E. colicells coexpressing a class IIB electron supply system. In the course of the isolation of the dxnA1 and dxnA2 cistrons, a number of other catabolic genes dispersed over different genome segments were identified, which may indicate greater catabolic potential than was previously suspected. This finding is consistent with the catabolic versatility of members of the genusSphingomonas, which is becoming increasingly evident, and may indicate a less well evolved and regulated but more dynamic genetic organization in this organism than is the case for better-studied pathways in organisms such as Pseudomonas species.
Lung adenocarcinoma global profiling identifies type II transforming growth factor-beta receptor as a repressor of invasiveness.

RATIONALE Lung adenocarcinoma histology and clinical outcome are heterogeneous and associated with tumor invasiveness. OBJECTIVES We hypothesized that invasiveness is associated with a distinct molecular signature and that genes differentially expressed in tumor or adjacent stroma will identify cell surface signal transduction and matrix remodeling pathways associated with the acquisition of invasiveness in lung adenocarcinoma. MAIN RESULTS Microarray analysis of microdissected noninvasive bronchioloalveolar carcinoma (BAC) and invasive adenocarcinoma and adenocarcinoma-mixed type with BAC features identified transcriptional profiles of lung adenocarcinoma invasiveness. Among the signature set that was lower in adenocarcinoma-mixed compared with BAC was the type II transforming growth factor beta (TGF-beta) receptor, suggesting downregulation of TGFbetaRII is an early event in lung adenocarcinoma metastasis. Immunostaining in independently acquired specimens demonstrated a correlation between TbetaRII expression and length of tumor invasion. Repression of TGFbetaRII in lung cancer cells increased tumor cell invasiveness and activated p38 mitogen-activated protein kinases. Microarray analysis of invasive cells identified potential downstream mediators of TGFbetaRII with differential expression in lung adenocarcinomas. CONCLUSIONS The repression of type II TGF-beta receptor may act as a significant determinant of lung adenocarcinoma invasiveness, an early step in tumor progression toward metastasis.