Miracle-dz/UniFAR-FTUs huggingface dataset:
Multiairport Ground Holding Problem: A Computational Evaluation of Exact Algorithms

Congestion in the air traffic network is becoming an increasingly serious problem that causes inconvenience to passengers, losses to airlines and, last but not least, threats to airspace safety. One way of reducing the amount of congestion is to use Ground Holding policies, i.e., to impose on selected aircraft a ground holding prior to their departure so that congestion during peak periods of time may be smoothed away. In this paper we restrict our attention to the Multiairport Ground Holding problem, where congestion may arise only at the airports due to limited arrival capacity. There are a few algorithms that, under suitable hypotheses, find an "optimal" policy for the Multiairport Ground Holding problem. In this paper we evaluate and compare comput ationally three of them, namely, the one recently proposed by Vranas, Bertsimas and Odoni, the one suggested by Andreatta and Tidona and that due to Bertsimas and Stock. The computational evaluation is based on two sets of test problems. The first set consists of seven problems taken from the literature. The second set consists of 32 "realistic" test problems. The results indicate the superiority of the Bertsimas and Stock approach among the three models considered.
Lithium treatment in affective disorders: The significance of strongly elevated erythrocyte choline levels

In manic-depressive patients treated with lithium salts the transport of choline over the erythrocyte membrane is strongly inhibited, resulting in dramatically increased erythrocyte choline levels (for review see ref. 1). Whether or not there is a relationship between this effect and treatment response is not clear. Data on this issue are scarce possibly because the measurement of treatment response in lithium prophylaxis is very difficult and time consuming. Also the effect on erythrocyte choline is specific for lithium and not for manic-depressive illness. We will address here the question of a possible relation between erythrocyte choline and clinical effects of lithium.
Supportive presumptive diagnosis of Plasmodium vivax malaria. Thrombocytopenia and red cell distribution width.

OBJECTIVE To evaluate hemoglobin, leukocyte, platelet counts and red cell distribution width values during acute vivax malaria. METHODS This study, which comprises 90 symptomatic vivax malaria patients compared with 52 healthy controls, investigated hemoglobin, leukocyte, platelet counts and red cell distribution width values during acute disease prior to the treatment in vivax malaria, from May 2002 to December 2004 in Adana, Cukurova region, located in the southern part of Turkey, along the Mediterranean coast. RESULTS Mean values for hemoglobin, leukocyte and platelet counts in the vivax malaria group in our study were found to be significantly lower in comparison to the control group. Anemia and thrombocytopenia were also observed in the malaria group while not in the control group (p<0.05, p<0.0001). Mean red cell distribution width values were found to be significantly higher in the malaria group (p<0.0001). CONCLUSION Our findings indicated that routinely used laboratory findings such as low hemoglobin, leukocyte or platelet counts and especially high red cell distribution width values could present a more supportive clue in the diagnosis of vivax malaria in endemic areas.
Dual substrate removal by an axenic bacterial culture

A pure bacterial culture capable of utilizing either L‐lysine or 2‐chlorophenol (2‐CP) as sole carbon source was isolated and used in continuous culture experiments to determine its response to dual substrate limitation by those two compounds. Dilution rate and feed composition were each set at three levels in a two factorial experimental design. The total chemical oxygen demand (COD) of the feed was fixed at 225 mg/L and its composition was varied by changing the ratio of lysine to 2‐CP. The effects of the two independent variables (dilution rate and feed composition) on the concentrations of cells, lysine, COD, and dissolved organic carbon (DOC) in the reactors were systematic whereas the effects on the 2‐CP concentration were less predictable. The concentrations of the two substrates responded to the two independent variables in a complex interactive manner which is not explained by existing models for dual, substitutable substrates. Rather, the results suggested that the prediction of the fate of a single organic component in a reactor receiving a multicomponent feed is a very difficult task.
Dual polarization THz time domain spectroscopy system

In many THz Time Domain Spectroscopy configurations, the separate acquisition of background and sample measurements have significant impact on data quality due to the need to perform subsequent measurements as well as the change in beam coupling geometry due to the removal of the sample in the reference measurement. In this short paper, we report on the design and build of a dual-polarization TDS system to perform synchronous background/sample measurements. This is achieved through the splitting of the PCA-generated THz beam with a THz polarizer to obtain two orthogonal polarized beams which are balanced and modulate the indices of two separate crystals on which the probe beam (also split) is reflected off before being detected. The two branches of the system show good similarity, and can be used to perform background and sample measurements synchronously, thus not being affected by changes in temperature and humidity, as well as small fluctuations in source power. Small differences in detector optical efficiency can be calibrated out at the start of a measurement session.
The surgical anatomy of the relationships between the perforating and the leptomeningeal arteries.

OBJECTIVE Despite detailed studies of the perforating arteries, their relationships with the leptomeningeal arteries are almost unknown. These relationships can be of great significance during neurosurgical operations. METHODS The arteries of the hemispheres, which ranged in number from 17 to 36, were injected with india ink or methylmethacrylate. RESULTS The perforating vessels were noted to arise from the following leptomeningeal arteries: the subcallosal branch of the anterior communicating artery (26.6%); the median artery of the corpus callosum (6.6%); the medial orbitofrontal (6.6%) and the olfactory branch (3.3%) of the anterior cerebral artery; the accessory middle cerebral artery (3.3%); the frontal and temporal branches of the middle cerebral artery (66.6%); the temporal branches of the internal carotid and the anterior choroidal arteries (25% each); the peduncular branch of the posterior communicating artery (4.8%); the peduncular, collicular and medial posterior choroidal branches of the posterior cerebral artery (40%); the cerebellar branches (100%); the long pontine branches (20-26.6%); the anterolateral branches (33.3%) of the basilar artery; and the anterolateral or the lateral medullary branches (35.3%) of the vertebral artery. From 19.4 to 100% of some leptomeningeal vessels originated in the large perforating arteries. CONCLUSION From 4.8 to 100% of certain groups of the perforating vessels originated in the leptomeningeal arteries. Occlusion of a leptomeningeal artery that gives rise to the perforating vessel(s) may lead to superficial and deep infarcts in the same patient.
Lipoarabinomannan Localization and Abundance during Growth of Mycobacterium smegmatis

ABSTRACT Lipoarabinomannan (LAM) is a structurally heterogeneous amphipathic lipoglycan present in Mycobacterium spp. and other actinomycetes, which constitutes a major component of the cell wall and exhibits a wide spectrum of immunomodulatory effects. Analysis of Mycobacterium smegmatis subcellular fractions and spheroplasts showed that LAM and lipomannan (LM) were primarily found in a cell wall-enriched subcellular fraction and correlated with the presence (or absence) of the mycolic acids in spheroplast preparations, suggesting that LAM and LM are primarily associated with the putative outer membrane of mycobacteria. During the course of these studies significant changes in the LAM/LM content of the cell wall were noted relative to the age of the culture. The LAM content of the M. smegmatis cell wall was dramatically reduced as the bacilli approached stationary phase, whereas LM, mycolic acid, and arabinogalactan content appeared to be unchanged. In addition, cell morphology and acid-fast staining characteristics showed variations with growth phase of the bacteria. In the logarithmic phase, the bacteria were found to be classic rod-shaped acid-fast bacilli, while in the stationary phase M. smegmatis lost the characteristic rod shape and developed a punctate acid-fast staining pattern with carbolfuchsin. The number of viable bacteria was independent of LAM content and phenotype. Taken together, the results presented here suggest that LAM is primarily localized with the mycolic acids in the cell wall and that the cellular concentration of LAM in M. smegmatis is selectively modulated with the growth phase.
Fixed or loose? Dichotomy in RSA data for cemented cups

Background and purpose Roentgen stereometric analysis (RSA) cannot discern whether a single prosthesis is fixed or migrating below the detection level. Samples of patients usually show migration values that appear to be continuously distributed. Is there such continuity, or is there a dichotomy between stable and migrating prostheses? The hypothesis of a dichotomy has, to our knowledge, not been tested. We present an exploratory evaluation of such a dichotomy using a mixture distribution algorithm. Methods We analyzed the migration (as determined by RSA) of 147 cemented acetabular cups of 7 different designs by using a new set of algorithms for frequency distribution analysis called Rmix. Results We first analyzed a migration vector, regardless of direction. After 2 years there was a significant dichotomy between 2 lognormal subgroups within the sample. Although some types of cups were over‐represented in one of the subgroups, neither cup design, sex, nor operating department could explain the dichotomy into two groups, which appears to reflect the existence of two basically different types of behavior of the cups. We next analyzed the migration along the 3 axes in space, and found a similar dichotomy. During the second year, around 80% of the patients belonged to a distinct, normally distributed subgroup with a mean not different from 0 mm and a small variation. The remainder differed significantly from this subgroup and showed migration. Interpretation There is a dichotomy in migration pattern. During the second postoperative year, most cups belonged to a subpopulation that appeared stable. The remainder is probably at risk of loosening. For a single type of prosthesis, the relative size of the stable subgroup may be a good index of the expected performance. The possibility of detecting subgroups within a seemingly continuous sample might be useful in many fields of medicine.
Nucleoprotein‐specific nonneutralizing antibodies speed up LCMV elimination independently of complement and FcγR

CD8+ T cells have an essential role in controlling lymphocytic choriomeningitis virus (LCMV) infection in mice. Here, we examined the contribution of humoral immunity, including nonneutralizing antibodies (Abs), in this infection induced by low virus inoculation doses. Mice with impaired humoral immunity readily terminated infection with the slowly replicating LCMV strain Armstrong but showed delayed virus elimination after inoculation with the faster replicating LCMV strain WE and failed to clear the rapidly replicating LCMV strain Docile, which is in contrast to the results obtained with wild‐type mice. Thus, the requirement for adaptive humoral immunity to control the infection was dependent on the replication speed of the LCMV strains used. Ab transfers further showed that LCMV‐specific IgG Abs isolated from LCMV immune serum accelerated virus elimination. These Abs were mainly directed against the viral nucleoprotein (NP) and completely lacked virus neutralizing activity. Moreover, mAbs specific for the LCMV NP were also able to decrease viral titers after transfer into infected hosts. Intriguingly, neither C3 nor Fcγ receptors were required for the antiviral activity of the transferred Abs. In conclusion, our study suggests that rapidly generated nonneutralizing Abs specific for the viral NP speed up virus elimination and thereby may counteract T‐cell exhaustion.
Effects on Proliferation and Differentiation of Multipotent Bone Marrow Stromal Cells Engineered to Express Growth Factors for Combined Cell and Gene Therapy

A key mechanism for mesenchymal stem cells/bone marrow stromal cells (MSCs) to promote tissue repair is by secretion of soluble growth factors (GFs). Therefore, clinical application could be optimized by a combination of cell and gene therapies, where MSCs are genetically modified to express higher levels of a specific factor. However, it remains unknown how this overexpression may alter the fate of the MSCs. Here, we show effects of overexpressing the growth factors, such as basic fibroblast growth factor (bFGF), platelet derived growth factor B (PDGF‐BB), transforming growth factor β1 (TGF‐β1), and vascular endothelial growth factor (VEGF), in human bone marrow‐derived MSCs. Ectopic expression of bFGF or PDGF‐B lead to highly proliferating MSCs and lead to a robust increase in osteogenesis. In contrast, adipogenesis was strongly inhibited in MSCs overexpressing PDGF‐B and only mildly affected in MSCs overexpressing bFGF. Overexpression of TGF‐β1 blocked both osteogenic and adipogenic differentiation while inducing the formation of stress fibers and increasing the expression of the smooth muscle marker calponin‐1 and the chondrogenic marker collagen type II. In contrast, MSCs overexpressing VEGF did not vary from control MSCs in any parameters, likely due to the lack of VEGF receptor expression on MSCs. MSCs engineered to overexpress VEGF strongly induced the migration of endothelial cells and enhanced blood flow restoration in a xenograft model of hind limb ischemia. These data support the rationale for genetically modifying MSCs to enhance their therapeutically relevant trophic signals, when safety and efficacy can be demonstrated, and when it can be shown that there are no unwanted effects on their proliferation and differentiation. STEM CELLS 2011;29:1727–1737
Antioxidant Potential of the Extracts, Fractions and Oils Derived from Oilseeds

The polyphenolic extracts and oils were obtained from ajwain, mustard, fenugreek and poppy seeds. The extracts were partitioned into acidic and neutral polyphenolic fractions and following estimation of total phenolics in the crude extract, acidic and neutral fractions and oil, all were analyzed for their DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging potential, ferric reducing ability and chelating power. The highest amount of polyphenols was found in ajwain (8330 ± 107), then in mustard seeds (2844 ± 56.00) and in fenugreek (1130 ± 29.00), and least in poppy seeds (937 ± 18.52). The higher amounts of polyphenols were estimated in neutral fraction compared to acidic (p < 0.05). % Inhibition of DPPH by the crude extract and fractions of all oilseeds was quite significant, being higher for acidic than neutral. The highest % DPPH inhibition was shown by ajwain extract than mustard > fenugreek and least by poppy seed extracts (p < 0.05). The reducing power and the chelating effect of the oilseeds followed the same order as DPPH, but higher % chelation was shown by neutral than acidic fraction (p < 0.05). Though low in polyphenols, the oil fractions were as strong antioxidants as the acidic one. Though oilseeds are used in very small quantity in food, they are potential sources of natural antioxidants and may replace synthetic ones.
Non-normality, Uncertainty and Inflation Forecasting: An Analysis of China's Inflation

Economic forecasting is important because it can affect the decision making processes of individuals, firms and governments so as to affect their behaviours. In this thesis, I discuss different methodologies for forecasting and forecast evaluation. I also discuss the role of assumption of normality and the role of uncertainty in economic forecasting. The first chapter is the introduction of the thesis. In second chapter, I conduct a Monte Carlo simulation to investigate the performances of forecast combination and the forecast encompassing test under the forecast errors non-normality. In third chapter, I examines the relationship between inflation forecast uncertainties and macroeconomic uncertainty for China by using different measures of uncertainties. I also investigate the relationship between inflation forecast uncertainties and inflation itself. In fourth chapter, I compute the probabilities of deflation for China by applying density forecast based on the theories and methodologies from previous two chapters. Particularly, I construct density forecasts for different forecast horizons by a joint distribution using Student-t copula. The fifth chapter is conclusion.
Distinct epitopes recognized by I-Ad-restricted T-cell clones within antigenic site E on influenza virus hemagglutinin

A total of 14 I-Ad-restricted helper T-cell clones specific for the hemagglutinin (HA) molecule of influenza virus were isolated from spleens of BALB/c or (BALB/c X C57BL/10)F1 mice immunized with the H3 subtype influenza virus A/Memphis/71 (Mem 71) and from lymph nodes of BALB/c mice primed with purified HA. The specificity of these T-cell clones was assessed in proliferation assays by reactivity with naturally occurring strains of viruses that arose by antigenic drift and contain known amino acid sequence changes in HA and with a panel of monoclonal antibody (MAb)-selected mutants of Mem 71 with single amino acid substitutions in HA. The HA genes of those mutant viruses that failed to stimulate one or more of the T-cell clones were sequenced. The clones could be allocated to at least four groups, each group having a distinct pattern of reactivity with the panel of natural field strains. The epitopes recognized by the four groups of clones were found, by reactivity with MAb-selected mutants, to be in very close proximity to one another and probably overlapping. All of the distinct epitopes recognized by the T-cell clones were adversely affected by a single amino acid substitution, either at residue 60 or at residue 63 in the HA1 polypeptide chain, within the region known from antibody-binding studies as site E. Some, but not all, of the epitopes may be influenced by the addition of a carbohydrate side chain to the HA of a particular MAb-selected mutant and certain field strains containing an Asp----Asn substitution at residue 63. Site E is therefore a major site of H-2d helper T-cell recognition on the H3 HA.
Risk of malignancy in patients with rheumatoid arthritis after anti-tumor necrosis factor therapy: results from Korean National Health Insurance claims data

Background/Aims Inhibition of tumor necrosis factor (TNF) is an effective treatment for rheumatoid arthritis (RA), but safety concerns about malignancy remain. The aim of this study was to evaluate cancer risk in RA patients treated with TNF inhibitors (TNFi), based on Korean Nationwide Health Insurance claims data. Methods Patients with seropositive RA were selected from the health insurance database containing all citizens’ medical information, based on both RA diagnosis codes and medications. Between 2010 and 2014, RA patients treated with conventional synthetic disease-modifying anti-rheumatic drugs (csDMARDs) and TNFi were enrolled and followed up. We compared the cancer incidence between patients treated with TNFi and csDMARDs using incidence rate ratios (IRRs) after adjustment for age, gender, and observational periods. Results Of 45,423 selected patients with seropositive RA, 2,337 were treated with TNFi and 43,086 were treated with csDMARDs. The TNFi group was younger and was followed-up for a longer duration. During the observational period, 1,732 and 49 cases of cancer were detected in patients treated with csDMARDs and TNFi, respectively. Old age and male sex were associated with cancer occurrence. Adjusted IRRs for all cancers and common cancers demonstrated that cancer incidence did not differ significantly between the TNFi group and csDMARDs group (IRR = 0.913 for all cancers, p = 0.546). Conclusions This study revealed that cancer incidence was similar in RA patients treated with TNFi and csDMARDs. Anti-TNF therapy may be a safe therapeutic option for RA treatment, in terms of malignancy.
Aliskiren reduces prorenin receptor expression and activity in cultured human aortic smooth muscle cells

Introduction: The recent discovery of a specific receptor for renin/prorenin (PRR) has added new interest to the potential pharmacological actions of aliskiren, the first direct renin inhibitor. Materials and methods: In the present study, to gain new insights into the pharmacological properties of aliskiren, we investigated the effect of aliskiren on PRR expression and activity in cultured human smooth muscle cells (HSMCs). Results: Co-incubation of HSMCs with angiotensinogen (ANG) (1.5× 10-7M) and prorenin (10-8–10-7M) resulted in an efficient production (within 4h) of angiotensin I, almost completely inhibited by 10-5M aliskiren (-86.0 ± 14.0%). In HSMCs stimulated with both ANG and prorenin, a 24h incubation with aliskiren (10-6–10-5M) resulted in a concentration-dependent reduction of PRR mRNA levels (IC50 4.6 × 10-6M). The cell surface expression of PRR determined by flow cytometry analysis was also reduced after incubation with aliskiren in a concentration-dependent manner. The lower levels of PRR were associated with a reduced expression of TGF-β, PAI-1 and type I collagen mRNA. Conclusions: These results suggest a direct pharmacological action of aliskiren on PRR expression and its signalling pathway in HSMCs. This reported action of aliskiren may reveal a new scenario of the pharmacological properties of aliskiren.
Typical noise exposure in daily life

Abstract Objective: Identify the distribution of typical noise levels present in daily life and identify factors associated with average sound levels. Design: This was an observational study. Study sample: Participants (N = 286) were 20 to 68 year old men and women, drawn from the general population of Kalamazoo County, Michigan. A total of 73 000 person-hours of noise monitoring were conducted. Results: Median overall daily average levels were 79 and 77 dBLeqA,8,equiv , with average levels exceeding EPA recommended levels for 70% of participants. Median levels were similar between the hours of 9 a.m. and 9 p.m. , and varied little across days of the week. Gender, occupational classification, and history of occupational noise exposure were related to average noise levels, but age, educational attainment, and non-occupational noise exposures were not. Conclusions: A large portion of the general population is exposed to noise levels that could result in long-term adverse effects on hearing. Gender and occupation were most strongly related to exposure, though most participants in this study had occupations that are not conventionally considered noisy.