Miracle-dz/UniFAR-FTUs huggingface dataset:
Combination of copeptin and troponin assays to rapidly rule out non-ST elevation myocardial infarction in the emergency department.

OBJECTIVES The aim of this study was to analyze the diagnostic accuracy and the clinical usefulness of the combination of troponin I (cTnI) and copeptin measured at presentation with an automated assay to rapidly rule out non-ST elevation myocardial infarction (NSTEMI) in patients with suspected cardiac chest pain presenting to an emergency department (ED). METHODS This study was an ancillary analysis of a prospective observational study. Copeptin and cTnI levels were sampled at presentation in 641 consecutive patients admitted to the ED for chest pain with onset within the last 12 hours and without ST elevation on a 12-lead electrocardiogram (ECG). Copeptin was measured with an automated assay and troponin with conventional assay. The performance of a combination of cTnI and copeptin for NSTEMI diagnosis was studied, the clinical utility was assessed by multivariate analysis, and an area under the curve (AUC) calculation was used to determine accuracy. RESULTS NSTEMI was diagnosed in 95 patients (15%). The sensitivity and negative predictive value (NPV) of the combination of copeptin and cTnI measures were 90.4% (95% confidence interval [CI] = 88.2% to 92.7%) and 97.6% (95% CI = 96.4% to 98.7%) versus 55.3% (95% CI = 51.5% to 59.2%) and 92.8% (95% CI = 90.8% to 94.8%) with cTnI alone. The AUC of the combination of copeptin and cTnI was 0.89 (95% CI = 0.85% to 0.92%) and was significantly higher than the AUC of cTnI alone (0.77, 95% CI = 0.72% to 0.82%, p < 0.05). The patient classification was slightly improved when copeptin was added to the usual diagnostic tools used for NSTEMI management. CONCLUSIONS In this study, determination of copeptin, in addition to cTnI, improves early diagnostic accuracy of NSTEMI. However, the sensitivity of this combination even using a conventional troponin assay remains insufficient to safely rule out NSTEMI at the time of presentation.
‘You just got to eat healthy’: The topic of CAM in the general practice consultation

Abstract New Zealand research suggests that CAM use by GPs has decreased, while referral to CAM practitioners by GPs has increased, and that patients often do not tell their health practitioners when they are using CAM. The New Zealand Medical Council has developed guidelines for GPs who use CAM. However, there is no research in New Zealand that looks at how patients and GPs respond to CAM issues in the consultation. This paper uses data collected for two research projects on doctor – patient interaction. For this research, consultations between 105 patients and nine GPs were video-recorded. In this data set, all doctors but one were ‘orthodox’ and to some degree reserved judgement on CAM, albeit remaining cautious in how they made this evident. Patients on the other hand demonstrated a variety of strategies to get CAM on the agenda, and GPs were careful to couch any criticism in such a way as to protect the face’ of patients.
Social factors associated with chest pain in the colored South Africans: results from the CRISIC study

Factors associated with chest pain were investigated in an independent dataset in the colored South Africans outside hospitals. Data were extracted from a cross-sectional study during 1982 in the Cape Peninsula. Information on lifestyle was obtained by interview. Regression modelings were performed. In multivariate analysis, only age (OR 1.54, 95%CI 1.11 to 2.14), mother having high blood pressure (OR 1.53, 95%CI 1.10 to 2.12), and father having stroke (OR 2.02, 95%CI 1.14 to 3.57) remained in the model. The results provide insight into the role of social factors that should be considered in the identification of future prevention and treatment.
Protective effect of wild Corni fructus methanolic extract against acute alcoholic liver injury in mice

ABSTRACT Background: In Chinese folk medicine, Corni fructus (C. fructus) has traditionally been used to improve liver function, although the mechanism underlying its activity remains unclear. The aim of the present study was to evaluate the protective effects of wild C. fructus methanolic extract against acute alcoholic liver injury. Methods: Alcohol was administered to mice for three consecutive days, either alone or in combination with C. fructus methanolic extract (50, 100, or 200 mg/kg body weight/d). Serum and liver tissue were collected from the animals and subjected to biochemical and histopathological analyses. Results: C. fructus signiﬁcantly alleviated alcohol-induced liver injury by reducing serum alanine aminotransferase, aspartate aminotransferase, and thiobarbituric acid reactive species, inhibiting hydroxyl radicals (•OH), and increasing total superoxide dismutase, glutathione peroxidase, and glutathione in the liver (P < 0.05). In addition, the C. fructus treatment inhibited the expression and activity of cytochrome P450 2E1 (P < 0.05). Conclusions: C. fructus could be a promising natural substance for ameliorating acute alcohol-induced oxidative stress and hepatic injury. GRAPHICAL ABSTRACT
Morinda citrifolia mitigates rotenone-induced striatal neuronal loss in male Sprague-Dawley rats by preventing mitochondrial pathway of intrinsic apoptosis

ABSTRACT Objectives: Parkinson disease (PD) is a neurodegenerative disorder affecting mainly the motor system, as a result of death of dopaminergic neurons in the substantia nigra pars compacta. The present scenario of research in PD is directed to identify novel molecules that can be administered individually or co-administered with L-Dopa to prevent the L-Dopa-Induced Dyskinesia (LID) like states that arise during chronic L-Dopa administration. Hence, in this study, we investigated whether Morinda citrifolia has therapeutic effects in rotenone-induced Parkinson’s disease (PD) with special reference to mitochondrial dysfunction mediated intrinsic apoptosis. Methods: Male Sprague-Dawley rats were stereotaxically infused with rotenone (3 µg in both SNPc and VTA) and co-treated with the ethyl acetate extract of Morinda citrifolia and levodopa. Results: The results revealed that rotenone-induced cell death was reduced by MCE treatment as measured by decline in the levels of pro-apoptotic proteins. Moreover, MCE treatment significantly augmented the levels of anti-apoptotic Bcl2 and blocks the release of cytochrome c, thereby alleviating the rotenone-induced dopaminergic neuronal loss, as evidenced by tyrosine hydroxylase (TH) immunostaining in the striatum. Discussion: Taken together, the results suggest that Morinda citrifolia may be beneficial for the treatment of neurodegenerative diseases like PD.
Galangin, a dietary flavonoid, improves antioxidant status and reduces hyperglycemia-mediated oxidative stress in streptozotocin-induced diabetic rats

ABSTRACT Objective: To examine the effect of galangin on hyperglycemia-mediated oxidative stress in streptozotocin (STZ)-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal administration of low-dose STZ (40 mg/kg body weight (BW)) into male albino Wistar rats. Galangin (8 mg/kg BW) or glibenclamide (600 µg/kg BW) was given orally, once daily for 45 days to normal and STZ-induced diabetic rats. Results: Diabetic rats showed significantly increased levels of plasma glucose, thiobarbituric acid reactive substances, lipid hydroperoxides, and conjugated dienes. The levels of insulin and non-enzymatic antioxidants (vitamin C, vitamin E, reduced glutathione) and the activity of enzymatic antioxidants (superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase (GST)) were decreased significantly in diabetic control rats. These altered plasma glucose, insulin, lipid peroxidation products, enzymatic and non-enzymatic antioxidants ions were reverted to near-normal level after the administration of galangin and glibenclamide. Conclusion: The present study shows that galangin decreased oxidative stress and increased antioxidant status in diabetic rats, which may be due to its antidiabetic and antioxidant potential.
Expression of Gpr177, a Wnt trafficking regulator, in mouse embryogenesis

Wls/Evi/Srt encoding a multipass transmembrane protein has been identified as a regulator for proper sorting and secretion of Wnt in flies. We have previously demonstrated that Gpr177 is the mouse ortholog required for axis determination. Gpr177 is a transcriptional target of Wnt that is activated to assist its subcellular distribution in a feedback regulatory loop. We, therefore, proposed that reciprocal regulation of Wnt and Gpr177 is essential for the Wnt‐dependent developmental and pathogenic processes. Here, we examine the expression pattern of Gpr177 in mouse development. Gpr177 is expressed in a variety of tissues and cell types during organogenesis. Furthermore, Gpr177 is a glycoprotein primarily accumulating in the Golgi apparatus in signal‐producing cells. The glycosylation of Gpr177 is necessary for proper transportation in the secretory pathway. Our findings suggest that the Gpr177‐mediated regulation of Wnt is crucial for organogenesis in health and disease. Developmental Dynamics 239:2102–2109, 2010. © 2010 Wiley‐Liss, Inc.
Mice lacking the orphan receptor ror1 have distinct skeletal abnormalities and are growth retarded

Ror1 is a member of the Ror‐family receptor tyrosine kinases. Ror1 is broadly expressed in various tissues and organs during mouse embryonic development. However, so far little is known about its function. The closely related family member Ror2 was shown to play a crucial role in skeletogenesis and has been shown to act as a co‐receptor for Wnt5a mediating non‐canonical Wnt‐signaling. Previously, it has been shown that during embryonic development Ror1 acts in part redundantly with Ror2 in the skeletal and cardiovascular systems. In this study, we report that loss of the orphan receptor Ror1 results in a variety of phenotypic defects within the skeletal and urogenital systems and that Ror1 mutant mice display a postnatal growth retardation phenotype. Developmental Dynamics 239:2266–2277, 2010. © 2010 Wiley‐Liss, Inc.
‘They haven’t a clue!’ A qualitative study of staff perceptions of 11–14 year-old female clinic attenders

Against a background of decreasing age at sexual debut, local Brook staff voiced concerns about the increase in clinic attenders under the age of 14 years, who appeared to engage in risky behaviours. This exploratory study, carried out with service providers in a young persons sexual health clinic, aimed to discover what issues staff felt were important to 11–14 year-old girls, in order that a future study be conducted with the young girls themselves. Service providers from two Brook Advisory Centres were interviewed. The interviews were semi-structured and analysed using content analysis. Many of the issues that staff identified as important to young girls mirrored current research findings with older adolescents. These were: alcohol consumption, peer pressure, low self-esteem and confidence, and a lack of knowledge around sexually transmitted infections. However, a new issue to emerge was the high proportion of young girls attending the clinic for the emergency contraceptive pill despite not knowing whether they had had intercourse or not. The study also found that staff perceived the need to understand issues from the perspective of the young girls themselves. Staff identified a necessity for improved sex education and education around peer pressure and assertiveness, whilst topics for future research which emerged from the study findings included issues of self-esteem, dynamics of peer/media pressure and why young girls feel that they need to have sex.
The Context of Risk Management in Mental Health Social Work

Managing risk is a key component of mental health social work practice, with the literature detailing two unique approaches to risk management — the dominant risk minimisation strategies and the less favoured risk-taking approaches. Due to a lack of research it is unclear, however, whether this is a practice reality, how professionals reconcile the tension between the two approaches and practice dilemmas, and the impact of wider factors perceived to influence these decisions. This paper aims to address these questions by drawing on 2010 research that undertook qualitative semi-structured interviews with seven Mental Health Officers in a Scottish local authority. Whilst the study found risk was generally constructed as relating to harm and danger, in practice a more measured approach to risk management was identified, with both approaches being employed, and a new acceptance of risk as potentially positive by organisations and practitioners was recognised. Participants illustrated how decisions are reached, without feeling inhibited by the ‘blame culture’, but clarified that this involved dilemmas and was a fraught area of practice. The paper concludes that more scope for positive risk-taking is desirable and requires the support of the policy context, legislation and organisations.
Stable, conditional, and muscle‐fiber‐specific expression of electroporated transgenes in chick limb muscle cells

Limb muscle formation is spread out over time and, consequently, muscle cells are not easy to target at any particular stages. We aimed to design a technique to study gene function in the different steps of limb muscle formation. We have associated transposon‐mediated gene transfer and a tetracycline‐dependent activation method with forelimb somite electroporation. In addition, we have established a new vector combining a differentiated‐muscle‐cell‐specific promoter and the transposon system, which allows stable gene expression in limb differentiated muscle cells and not in muscle progenitors. Using these methods, we observed that conditional Fgf4 expression in muscle cells at the onset of fetal muscle differentiation and specific Fgf4 expression in differentiated muscle cells alter muscle fiber formation in chick limbs. Limb somite electroporation with these set of vectors allowing stable, conditional, and differentiated‐muscle‐specific expression of transgenes opens new perspectives for investigating gene function at various steps of limb muscle formation. Developmental Dynamics, 2011. © 2010 Wiley‐Liss, Inc.
Pattern of acute blood transfusion reactions in Kano, North-Western Nigeria

Background : Advancement in transfusion medicine has led to the use of large quantities of blood and blood components in modern clinical practice. The purpose of this study is to determine the pattern of acute blood transfusion reactions among adults in North-Western Nigeria. Materials and Methods: This was a descriptive observational study over a 3-month period that evaluated 302 blood transfusions in 180 consenting recipients in Aminu Kano Teaching Hospital. Data was analyzed using descriptive and inferential statistics, and P -values of ≤ 0.05 were regarded as significant. Results: The mean age of the recipients was 37.2 ± 13.7 (95% CI: 35.2 - 39.2) years. The incidence of acute transfusion reaction (ATRs) was 3.6%, out of which 3.3% were febrile non-hemolytic transfusion reaction (FNHTR) and 0.3% were acute allergic reaction (AAR). All the recipients that reacted presented clinically with fever and more than 90% of them had associated chills and/or rigors. There was a significant relationship between ATRs and history of previous transfusion (χ 2 = 4.12; P = 0.04) and stored blood of more than 3 days (χ 2 = 5.20; P = 0.02). Conclusion: Patients with previous history of transfusion should be monitored closely as they have a higher risk of developing transfusion reaction, and transfusion of blood stored for more than 3 days should as much as possible be avoided, especially in patients that have a potential risk of transfusion reaction.
Short communication: A longitudinal study of serum adiponectin during normal pregnancy

Adiponectin is secreted from adipose tissue. Serum adiponectin levels are inversely correlated with body mass index (BMI) and also insulin resistance, independent of the BMI. A role for adiponectin in the development of insulin resistance has been implied in pregnancy. However, no studies have been performed to describe the individual longitudinal course of adiponectin in normal pregnancies. Therefore, we measured serum adiponectin during and after normal pregnancy in 11 healthy women. Serum levels peaked in midpregnancy and the lowest levels were seen in late pregnancy. An inverse association with maternal BMI was observed.
Embryonic development of the emu, Dromaius novaehollandiae

The chick, Gallus gallus, is the traditional model in avian developmental studies. Data on other bird species are scarce. Here, we present a comparative study of the embryonic development of the chick and the emu Dromaius novaehollandiae, a member of Paleognathae, which also includes the ostrich, rhea, tinamou, kiwi, and cassowary. Emu embryos ranging from Hamburger and Hamilton (HH) equivalent stages 1 to 43 were collected and their gross morphology analyzed. Its early development was studied in detail with time‐lapse imaging and molecular techniques. Emu embryos in general take 2–3 times longer incubation time to reach equivalent chicken stages, requiring 1 day for HH2, 2.5 days for HH4, 7 days for limb bud initiation, 23 days for feather germ appearance, and approximately 50–56 days for hatching. Chordin gene expression is similar in emu and chick embryos, and emu Brachyury is not expressed until HH3. Circulation is established at approximately the 27‐ to 30‐somite stage. Forelimb buds are formed and patterned initially, but their growth is severely retarded. The size difference between an emu and a chick embryo only becomes apparent after limb bud formation. Overall, emu and chick embryogenesis proceeds through similar stages, but developmental heterochrony between these two species is widely observed. Developmental Dynamics, 2011. © 2010 Wiley‐Liss, Inc.
A novel hypomorphic Looptail allele at the planar cell polarity Vangl2 gene

Vangl2 forms part of the planar cell polarity signalling pathway and is the gene defective in the Looptail (Lp) mouse mutant. Two previously described alleles, Lp and Lpm1Jus, segregate in a semi‐dominant fashion, with heterozygotes displaying the looped‐tail appearance, while homozygotes show the neural tube defect called craniorachischisis. Here, we report a novel experimentally induced allele, Lpm2Jus, that carries a missense mutation, R259L, in Vangl2. This mutation was specific to the Lp phenotype and absent from both parental strains and 28 other inbred strains. Notably, this mutation segregates in a recessive manner with all heterozygotes appearing normal and 47% of homozygotes showing a looped‐tail. Homozygous Lpm2Jus embryos showed spina bifida in 12%. Lpm2Jus genetically interacts with Lp with 77% of compound heterozygotes displaying craniorachischisis. Vangl2R259L behaved like the wild‐type allele in overexpression and morpholino knockdown/rescue assays in zebrafish embryos. These data suggest that Lpm2Jus represents a new hypomorphic allele of Lp. Developmental Dynamics 240:839–849, 2011. © 2011 Wiley‐Liss, Inc.
Control of pelvic girdle development by genes of the Pbx family and Emx2

Genes expressed in the somatopleuric mesoderm, the embryonic domain giving rise to the vertebrate pelvis, appear important for pelvic girdle formation. Among such genes, Pbx family members and Emx2 were found to genetically interact in hindlimb and pectoral girdle formation. Here, we generated compound mutant embryos carrying combinations of mutated alleles for Pbx1, Pbx2, and Pbx3, as well as Pbx1 and Emx2, to examine potential genetic interactions during pelvic development. Indeed, Pbx genes share overlapping functions and Pbx1 and Emx2 genetically interact in pelvic formation. We show that, in compound Pbx1;Pbx2 and Pbx1;Emx2 mutants, pelvic mesenchymal condensation is markedly perturbed, indicative of an upstream control by these homeoproteins. We establish that expression of Tbx15, Prrx1, and Pax1, among other genes involved in the specification and development of select pelvic structures, is altered in our compound mutants. Lastly, we identify potential Pbx1‐Emx2–regulated enhancers for Tbx15, Prrx1, and Pax1, using bioinformatics analyses. Developmental Dynamics 240:1173–1189, 2011. © 2011 Wiley‐Liss, Inc.
Stress and Deception in Speech: Evaluating Layered Voice Analysis *

Abstract: This study was designed to evaluate commonly used voice stress analyzers—in this case the layered voice analysis (LVA) system. The research protocol involved the use of a speech database containing materials recorded while highly controlled deception and stress levels were systematically varied. Subjects were 24 each males/females (age range 18–63 years) drawn from a diverse population. All held strong views about some issue; they were required to make intense contradictory statements while believing that they would be heard/seen by peers. The LVA system was then evaluated by means of a double blind study using two types of examiners: a pair of scientists trained and certified by the manufacturer in the proper use of the system and two highly experienced LVA instructors provided by this same firm. The results showed that the “true positive” (or hit) rates for all examiners averaged near chance (42–56%) for all conditions, types of materials (e.g., stress vs. unstressed, truth vs. deception), and examiners (scientists vs. manufacturers). Most importantly, the false positive rate was very high, ranging from 40% to 65%. Sensitivity statistics confirmed that the LVA system operated at about chance levels in the detection of truth, deception, and the presence of high and low vocal stress states.
Zebrafish nephrogenesis involves dynamic spatiotemporal expression changes in renal progenitors and essential signals from retinoic acid and irx3b

Kidney nephrons are composed of proximal and distal tubule segments that perform unique roles in excretion. The developmental pathways that establish nephron segment identities from renal progenitors are poorly understood. Here, we used the zebrafish pronephros to study nephron segmentation. We found that zebrafish nephron progenitors undergo elaborate spatiotemporal expression changes of many genes before adopting a segment fate. Initially, two domains of nephron progenitors are established, then are subdivided and demarcate individual nephron segments. Using genetic and chemical genetic models of retinoic acid (RA) deficiency, we discovered that RA modulates rostral progenitor formation. To delineate downstream pathways, we knocked down the irx3b transcription factor and found it regulates proximal tubule segment size and distal segment differentiation. Our results suggest a model whereby RA patterns the early field of nephron progenitors, with subsequent factors like irx3b acting to refine later progenitor subdomains and ensure activation of segment‐specific gene programs. Developmental Dynamics 240:2011–2027, 2011. © 2011 Wiley‐Liss, Inc.
l 1-gain performance analysis and positive filter design for positive discrete-time Markov jump linear systems : A linear programming approach

This paper is concerned with the l1-gain performance analysis and positive filter design for a positive discrete-time Markov jump linear system (MJLS) by using a linear programming (LP) approach. First, by constructing a linear stochastic Lyapunov function and introducing an ‘‘equivalent’’ deterministic positive discrete-time linear system, necessary and sufficient conditions in the form of LP are derived for stochastic stability and l1-gain performance of the positive discrete-timeMJLS. Then a sufficient condition on the existence of the desired positive l1-gain filter is provided. The desired positive l1-gain filter can be designed by solving a standard LP problem. The pest’s structured population dynamic model is employed to illustrate the effectiveness of the proposed method. © 2014 Elsevier Ltd. All rights reserved.