Miracle-dz/UniFAR-FTUs huggingface dataset:
Protection of intestinal injury during heat stroke in mice by interleukin‐6 pretreatment

Heat stroke afflicts thousands of humans each year, worldwide. The immune system responds to hyperthermia exposure resulting in heat stroke by producing an array of immunological proteins, such as interleukin‐6 (IL‐6). However, the physiological functions of IL‐6 and other cytokines in hyperthermia are poorly understood. We hypothesized that IL‐6 plays a protective role in conditions of heat stroke. To test this, we gave small IL‐6 supplements to mice prior to exposing them to hot environments sufficient to induce conditions of heat stroke. Pretreatment with IL‐6 resulted in improved ability to withstand heat exposure in anaesthetized mice, it protected the intestine from injury, reducing the permeability of the intestinal barrier, and it attenuated the release of other cytokines involved in inflammation. The results support the hypothesis that IL‐6 is a ‘physiological stress hormone’ that plays an important role in survival during acute life‐threatening conditions such as heat stroke.
Crystal Structure of Unsaturated Glucuronyl Hydrolase, Responsible for the Degradation of Glycosaminoglycan, from Bacillus sp. GL1 at 1.8 Å Resolution*

Unsaturated glucuronyl hydrolase (UGL) is a novel glycosaminoglycan hydrolase that releases unsaturated d-glucuronic acid from oligosaccharides produced by polysaccharide lyases. The x-ray crystallographic structure of UGL from Bacillus sp. GL1 was first determined by multiple isomorphous replacement (mir) and refined at 1.8 Å resolution with a final R-factor of 16.8% for 25 to 1.8 Å resolution data. The refined UGL structure consists of 377 amino acid residues and 478 water molecules, four glycine molecules, two dithiothreitol (DTT) molecules, and one 2-methyl-2,4-pentanediol (MPD) molecule. UGL includes an α6/α6-barrel, whose structure is found in the six-hairpin enzyme superfamily of an α/α-toroidal fold. One side of the UGL α6/α6-barrel structure consists of long loops containing three short β-sheets and contributes to the formation of a deep pocket. One glycine molecule and two DTT molecules surrounded by highly conserved amino acid residues in UGLs were found in the pocket, suggesting that catalytic and substrate-binding sites are located in this pocket. The overall UGL structure, with the exception of some loops, very much resembled that of the Bacillus subtilis hypothetical protein Yter, whose function is unknown and which exhibits little amino acid sequence identity with UGL. In the active pocket, residues possibly involved in substrate recognition and catalysis by UGL are conserved in UGLs and Yter. The most likely candidate catalytic residues for glycosyl hydrolysis are Asp88 and Asp149. This was supported by site-directed mutagenesis studies in Asp88 and Asp149.
Chronic progressive multiple sclerosis: Serial magnetic resonance brain imaging over six months

Eight patients in the chronic progressive stage of multiple sclerosis were studied prospectively with magnetic resonance imaging of the head and neurological examination every 2 weeks for 6 months. There were no clinical relapses and disability ratings did not change between the start and completion of the study. Despite the clinical inactivity, a total of 86 active events (new, reappearing, or enlarging lesions) were identified by magnetic resonance imaging over the 6 months, with 50 (51) of 98 follow‐up scans showing evidence of one or more active lesions. New and changing lesions were indistinguishable in appearance, distribution, and temporal pattern from those previously seen in patients who had relapsing and remitting multiple sclerosis. However, as noted previously, the patients in the chronic progressive stage had many more confluent lesions than did the group in relapse. These results strongly suggest that the frequently observed clinical evolution of multiple sclerosis into a chronic progressive course is not accompanied by a fundamental change in the disease process. In fact the chronic progressive stage seems associated with more active changes demonstrated by scans than does relapsing and remitting multiple sclerosis. This study also confirms our previous experience that serial magnetic resonance imaging is much more sensitive than clinical examination in detecting disease activity in multiple sclerosis.
Improved skeletal muscle oxidative enzyme activity and restoration of PGC-1 alpha and PPAR beta/delta gene expression upon rosiglitazone treatment in obese patients with type 2 diabetes mellitus.

OBJECTIVE To examine whether rosiglitazone alters gene expression of some key genes involved in mitochondrial biogenesis and oxidative capacity in skeletal muscle of type 2 diabetic patients, and whether this is associated with alterations in skeletal muscle oxidative capacity and lipid content. DESIGN measured in muscle biopsies obtained from diabetic patients, before and after 8 weeks of rosiglitazone treatment, and matched controls. Furthermore, whole-body insulin sensitivity and substrate utilization were assessed. SUBJECTS Ten obese type 2 diabetic patients and 10 obese normoglycemic controls matched for age and BMI. METHODS Gene expression and mitochondrial protein content of complexes I-V of the respiratory chain were measured by quantitative polymerase chain reaction and Western blotting, respectively. Histochemical staining was used to quantify lipid accumulation and complex II succinate dehydrogenase (SDH) activity. Insulin sensitivity and substrate utilization were measured during a hyperinsulinemic-euglycemic clamp with indirect calorimetry. RESULTS Skeletal-muscle mRNA of PGC-1 alpha and PPAR beta/delta--but not of other genes involved in glucose, fat and oxidative metabolism--was significantly lower in diabetic patients (P<0.01). Rosiglitazone significantly increased PGC-1 alpha ( approximately 2.2-fold, P<0.01) and PPAR beta/delta ( approximately 2.6-fold, P<0.01), in parallel with an increase in insulin sensitivity, SDH activity and metabolic flexibility (P<0.01). Surprisingly, none of the measured mitochondrial proteins was reduced in type 2 diabetic patients, nor affected by rosiglitazone treatment. No alterations were seen in muscular fat accumulation upon treatment. CONCLUSION These results suggest that the insulin-sensitizing effect of rosiglitazone may involve an effect on muscular oxidative capacity, via PGC-1 alpha and PPAR beta/delta, independent of mitochondrial protein content and/or changes in intramyocellular lipid.
Early increase in levels of soluble inter-cellular adhesion molecule-1 (sICAM-1); potential risk factor for the acute coronary syndromes.

BACKGROUND Studies have shown disparate results in relation to the role of plasma concentrations of cell adhesion molecules in atherosclerosis. Moreover, the differentiation of primary vs secondary alterations of these markers, in response to myocardial injury, has not been clear. We measured specific soluble cell adhesion molecules and inflammatory markers in men admitted acutely with chest pain and compared them to healthy controls. METHODS AND RESULTS We prospectively studied men (total n=241), admitted acutely with chest pain (7.4+/-9.4 h, 71% within 10 h), unstable angina (n=67), acute myocardial infarction (n=47) and chest pain without ischaemic heart disease (n=45) and compared them with a stratified sample of randomly selected healthy controls (n=82). Soluble intercellular adhesion molecule (sICAM-1), endothelial selectin, vascular cell adhesion molecule, interleukin-6 and C-reactive protein were measured by ELISA and P-selectin expression by flow cytometry. Multiple regression analysis was used to control for the impact of classical risk factors. At baseline ICAM-1, interleukin-6 and C-reactive protein were significantly elevated in patient groups whereas no difference in vascular cell adhesion molecule or endothelial selectin was found. At 3 month follow-up, ICAM-1 level was unchanged in ischaemic heart disease patients. In all groups C-reactive protein and interleukin-6 levels were lower at review. ICAM-1 levels at follow-up were higher in ischaemic heart disease groups (but not in chest pain without ischaemic heart disease) relative to controls and remained so only in the unstable angina group following regression. sICAM-1, interleukin-6 and C-reactive protein strongly correlated with smoking. In the acute phase, ICAM-1 was confounded by smoking following regression and C-reactive protein and interleukin-6 remained significant in both ischaemic heart disease groups after multiple regression. There was no relationship to events which occurred in 23% of ischaemic heart disease patients (further acute myocardial infarction 5.3%, sudden cardiac death 0.9% or recurrent angina 16.7%). CONCLUSION We found an inflammatory response with higher sICAM-1, interleukin-6 and C-reactive protein in patients presenting soon after developing an acute coronary syndrome. As sICAM-1 was not affected by the acute event this plasma marker may be an important risk factor for the development of the acute coronary syndrome, particularly unstable angina.
Active Intervention in Patients with Whiplash-Associated Disorders Improves Long-Term Prognosis: A Randomized Controlled Clinical Trial

Study Design. Three-year follow-up of a prospective randomized trial in 97 patients exposed to whiplash trauma in motor vehicle collisions. Objectives. To compare the long-term efficacy of active intervention with that of standard intervention and the effect of early versus delayed initiation of intervention. Summary of Background Data. There is no strong evidence for many treatments for whiplash-associated disorders. Some studies provide weak evidence supporting active intervention. Method. Patients were randomized to an intervention using frequent active cervical rotation complemented by assessment and treatment according to McKenzie’s principles or to a standard intervention of initial rest, recommended soft collar, and gradual self-mobilization. To test the time factor, interventions were either made within 96 hours or delayed 14 days from collision. The effects of the two interventions and the time factor on pain intensity, cervical range of motion, and sick leave were analyzed at 6 months and 3 years. Cervical range of motion at 3 years was also compared with that in matched, unexposed individuals. Results. Pain intensity and sick leave were significantly (P < 0.05) reduced if patients received active intervention compared with standard intervention. Delaying intervention 2 weeks did not affect outcome variables. However, at 3 years, only patients receiving early active intervention had a total cervical range of motion similar to that of matched unexposed individuals. Conclusion. In patients with whiplash-associated disorders, active intervention is more effective in reducing pain intensity and sick leave, and in retaining/regaining total range of motion than a standard intervention. Active intervention can be carried out as home exercises initiated and supported by appropriately trained health professionals.
The significance of political culture, economic context and instrument type for climate policy support: a cross-national study

ABSTRACT While many countries have pledged to reduce greenhouse gas (GHG) emissions, the choice of national climate policy measures demonstrates widespread variation. Although system of government, path-dependency and economic entanglements can explain a certain amount of variation in policy choice, research also points specifically towards the highly politicized nature of climate policy instruments and their sensitivity to public support as explanatory factors for cross-national differences. Previous studies hypothesize that various country-specific contextual factors determine both general preferences for environmental protection and the public’s preferences for different types of policy instruments. One suggestion is that countries’ prevailing political cultures have significant consequences for such public support. Another supposition is that, since countries differ in their economic dependency on climate detrimental industry such as fossil fuel production, this should be a significant factor determining both public attitudes and subsequent political decisions. This paper applies unique, original data from four countries with significant variation in (i) political-cultural contexts (Sweden and Norway vs New Zealand and Australia and (ii) economic dependency (Norway and Australia vs Sweden and New Zealand) to analyze how, and to what extent, these two contextual variables interact with, and moderate, the effect of individual-level factors on support for climate policy measures in the four countries. Furthermore, the paper explores variations in support for different types of CO2 taxes (directed towards individual consumers, industry, and fossil-fuel producers) in the four countries. Key policy insights Across contexts, public policy support is lower for taxes directed towards private consumption than for taxes directed towards industry, and the strongest for CO2 taxes on fossil fuel producing industry. Political culture and economic context influence the effect of individual-level factors on policy support. In a context of high economic dependency on the fossil-fuel industry, people are less likely to support the introduction of CO2 taxes. The effect of left-right ideology on policy support is sensitive to political-cultural context.
Cultural Tourism in a Replicated Old Town: Tourists’ Views

ABSTRACT The proliferation of replicated old towns for tourism purposes is widespread throughout China. Employing surveys, informal interviews, observations and secondary sources, this study explores tourists’ views of their experiences, including their perceptions of staged authenticity and levels of satisfaction at a newly built attraction in Yunnan, China. While some tourists hope to find objective authenticity in a simulated ethnic attraction, others look for fun and relaxation. Visitors experience mixed reactions, with greater satisfaction with the overall experience than with the specific qualities and authenticity of offerings. While some tourists are interested in accessing authentic culture, other aspects, such as enjoyment and relaxation, receive higher priority. Many visitors are concerned about over-commercialization, superficial cultural portrayals and a high concentration of tourist facilities. It is argued that increasing the level of authenticity may be a strategy to promote the overall attractiveness of the site.
A new central–peripheral corneal curvature method for intraocular lens power calculation after excimer laser refractive surgery

Purpose: To propose the central–peripheral (C‐P) method, which requires no data history to calculate intraocular lens (IOL) powers for eyes that underwent laser in situ keratomileusis (LASIK), and compare the accuracy of the C‐P method with other IOL formulas for eyes after LASIK.
Non‐invasive model predicting clinically‐significant portal hypertension in patients with advanced fibrosis

Background and Aims: Hepatic venous pressure gradient (HVPG) has been established as a predictor for the development of varices, clinical decompensation and death. In the present study, the primary objectives were to determine the diagnostic accuracy of the model developed by using readily‐available data in predicting the presence of significant portal hypertension and esophageal varices.
The Forebrain of Actinopterygians Revisited

The forebrain of actinopterygian fishes differs from that of other vertebrates in that it consists of a pair of solid lobes. Lateral ventricles surrounded by nervous tissue are entirely lacking. Comparative anatomical and embryological studies have shown that the unusual configuration of the forebrain in actinopterygians results from an outward bending or eversion of the dorsal portions of its lateral walls. Due to this eversion, the telencephalic roof plate is transformed into a wide, membranous structure which surrounds the dorsal and lateral parts of the solid lobes and is attached to their lateral or ventrolateral aspects. The taeniae, i.e. the lines of attachment of the widened roof plate, represent important landmarks in actinopterygian forebrains. In the present paper, the process of eversion is specified and quantified. It is pointed out that recent suggestions to modify the original eversion concept lack an empirical basis. Eversion is the antithesis of the inward bending or inversion that occurs in the forebrains of most other vertebrates. The forebrain lobes in actinopterygians, like those in other vertebrates, comprise a pallium and a subpallium, both of which include a number of distinct cell masses. The morphological interpretations of these cell masses over the past 130 years are reviewed and evaluated in light of a set of carefully selected criteria for homologous relationships. Special attention is paid to the interpretation of a cell mass known as Dp, situated in the caudolateral portion of the pallium in teleosts (by far the largest clade of living actinopterygians). Based on its position close to the taenia, and given the everted condition of the pallium in teleosts, this cell mass clearly corresponds with the medial pallium in inverted forebrains; however, Dp receives a dense olfactory input, and it shares this salient feature with the lateral pallium, rather than with the medial pallium of inverted forebrains. There is presently no consensus regarding the homology of Dp. Several recent authors [Wullimann and Mueller, 2004; Yamamoto et al., 2007] consider the lateral pallium in inverted forebrains and Dp in teleosts to be homologous because they believe that these cell masses originate from the same germinative zones, but that Dp attains its ultimate position only through migration. On the other hand, the present author believes that Dp is situated in the immediate vicinity of its germinative zone and that it represents a specialized part of the lateral pallial zone in teleosts, a zone that can be homologized topologically with the medial pallium in inverted forebrains. Further, it is proposed that the lateral olfactory tract in teleosts, which supplies most of the olfactory fibers to Dp, is not homologous to the same-named tract in the inverted forebrains of most other vertebrates.
Cardiac Shock Wave Therapy Attenuates H9c2 Myoblast Apoptosis by Activating the AKT Signal Pathway

Background: Previous studies have demonstrated that Cardiac Shock Wave Therapy (CSWT) improves myocardial perfusion and cardiac function in a porcine model of chronic myocardial ischemia and also ameliorates myocardial ischemia in patients with severe coronary artery disease (CAD). Apoptosis plays a key role in ischemic myocardial pathogenesis. However, it remains unclear whether CSWT is beneficial for ischemia/hypoxia (I/H)-induced myocardial cell apoptosis and by which mechanism CSWT could improve heart function. We put forward the hypothesis that CSWT might protect heart function during ischemia/hypoxia by decreasing apoptosis. Methods: We generated ischemia/hypoxia (I/H)-induced apoptosis in the H9c2 myoblast cell line to examine the CSWT function and possible mechanisms. H9c2 cells were treated under hypoxic serum-starved conditions for 24 h and then treated with or without CSWT (500 shots, 0.06, 0.09, 0.12mJ/mm2). The apoptotic cell rate was determined by flow cytometry assay, cell viability was examined by the MTT assay, nuclear fragmentation was detected by Hoechst 33342 staining, and the mitochondrial-mediated intrinsic pathway of apoptosis was assessed by the expression of Bax and Bcl-2 protein and Caspase3 activation. Results: First, apoptosis could be induced by ischemia/hypoxia in H9c2 cells. Second, CSWT attenuates the cell death and decreases the H9c2 cell apoptosis rate induced by ischemia and hypoxia. Third, CSWT suppresses the expression of apoptosis molecules that regulate the intrinsic pathway of apoptosis in H9c2 cells. Fourth, CSWT increases the phosphorylation of AKT, which indicates the activation of the PI3K-AKT pathway. Conclusions: These results indicate that CSWT exerts a protective effect against I/H-induced cell death, potentially by preventing the activation of components of the mitochondrial-dependent intrinsic apoptotic pathway. We also demonstrate that the PI3K-Akt pathway may be involved in the CSWT effects on apoptosis.
A theory for robust risk measures

In this paper, we address the uncertainty regarding the choice of a probability measure in the context of risk measures theory. To do that, we consider the notion of probability-based risk measurement and two general approaches to generate risk measures that are robust. The first approach is a worst-case situation, while the second one is based on weighted averaging. We develop results regarding issues on the theory of risk measures, such as financial and continuity properties, dual representations, law invariance and elicitability. Furthermore, we explore similar results for deviation measures under our framework.
Does Regulation Drive International Research Cooperation? Evidence from the Pharmaceutical Sector

To what extent is an increased stringency of the Intellectual Property Rights (IPR) system apt to stimulate research cooperation between developed and emerging economies? To address this question, we empirically investigate how international joint research projects in the pharmaceutical sector are affected by the regime of IPR in force in the two countries involved in the collaboration. Looking at the joint signature of both patent documents and scientific papers by researchers located in developed and emerging markets, our investigation indicates two opposite effects: joint publications are fostered by stricter IPR rules, whereas joint patents are discouraged. A recently proposed theory provides a plausible rationale for this apparently contradicting result.
HYBRIDIZATION COST OF DELAYED MATURATION OF SECONDARY SEXUAL TRAITS IN THE COLLARED FLYCATCHER

Abstract In many species, individuals do not attain their full adult coloration until one or several years after reaching sexual maturity, and this signaling of juvenile status is thought to enable young individuals to avoid aggression from older, dominant conspecifics. We propose that hybridization may be one of several costs and benefits associated with such delayed maturation. We tested this idea in a hybrid zone of collared (Ficedula albicollis) and pied (F. hypoleuca) flycatchers on the Baltic islands of Öland and Gotland. One-year-old (subadult) male collared flycatchers differed from older birds in many plumage traits, and approached male pied flycatchers in phenotype. On both islands, subadult male collared flycatchers hybridized at a higher rate than adults. Mate-choice experiments in aviaries suggest that this difference is at least partly due to female pied flycatchers having a preference for subadults when constrained to choose a heterospecific mate. Because novel morphologies are often derived from changes in ontology, juvenile forms may resemble adults of closely related taxa. When such juveniles are reproductively mature, their phenotypic similarity to the adults of closely related species may increase their risk of hybridization.
Development of a simple and rapid method for the specific identification of organism causing anthrax by slide latex agglutination

A specific latex agglutination test (LAT) based on anti‐PA (protective antigen) antibodies having detection limit of 5 × 104 formalin treated Bacillus anthracis cells or 110 ng of PA was optimized in this study. The optimized LAT could detect anthrax toxin in whole blood as well as in serum from the animal models of anthrax infection. The protocol is a simple and promising method for the specific detection of bacteria causing anthrax under routine laboratory, as well as in field, conditions without any special equipments or expertise.