Miracle-dz/UniFAR-FTUs huggingface dataset:
Integrin‐dependent response to laminin‐511 regulates breast tumor cell invasion and metastasis

The basement membrane protein, laminin (LM)‐511, is a potent adhesive and migratory substrate for metastatic breast tumor cells in vitro. Its expression correlates with tumor grade and metastatic potential in vivo. These observations suggest that responsiveness to autocrine or paracrine‐derived LM‐511 may be an important property regulating breast cancer metastasis in vivo. To address this, we compared the metastatic potential of 4T1 mammary carcinoma cells to that of 4T1 variants isolated by repeated chemotactic migration toward LM‐511 in vitro (4T1LMF4) followed by serial injection into the mammary gland and recovery of spontaneous metastases from bone (4T1BM2). Variant subpopulations exhibited a distinct morphology on LM‐511 and increased expression of β1 and β4 integrins compared to parental 4T1 cells. Importantly, mice inoculated with 4T1LMF4 and 4T1BM2 variants showed a 2.5‐ to 4‐fold increase in the incidence of spontaneous metastasis to bone compared to 4T1 tumor‐bearing mice. Functionally, 4T1BM2 variants were more adherent and more invasive toward LM‐511 than parental 4T1 cells. Treatment of 4T1BM2 cells with lebein‐1, a disintegrin with selectivity toward LM‐type integrin receptors, potently inhibited their migration and invasion toward LM‐511. Similarly, α3β1 integrin‐dependent migration and invasion of human MDA‐MB‐231 breast carcinoma cells toward LM‐511 were significantly inhibited by lebein‐1. Taken together, these results provide strong evidence that LM‐511 contributes to the metastasis of breast tumors and suggest that targeting integrin‐LM‐511 interactions with lebein‐1 or other inhibitors of LM‐511 receptors may have therapeutic potential for patients with advanced breast cancer.
Mlc Circuit in the Frame of CNN

A CNN-based nonautonomous chaotic oscillator circuit design is presented. Murali–Lakshmanan–Chua circuit, known as MLC circuit, is modeled by using CNN cells. The circuit implementation is supported by an eigenvalue study of the introduced system. The proposed model gives an alternative to MLC circuit with inductorless RC-based circuit realization.
Evaluation of Alternative Planting Strategies to Reduce Wheat Stem Sawfly (Hymenoptera: Cephidae) Damage to Spring Wheat in the Northern Great Plains

ABSTRACT Wheat, Triticum aestivum L. , producers are often reluctant to use solid-stemmed wheat cultivars resistant to wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), due to concerns regarding yield, efficacy or market opportunities. We evaluated the impact of several planting strategies on wheat yield and quality and wheat stem sawfly infestation at two locations over a three-year period. Experimental units consisted of large plots (50 by 200 m) located on commercial farms adjacent to wheat stem sawfly-infested fields. Compared with a monoculture of a hollow-stemmed cultivar (‘AC Barrie’), planting a monoculture of a solid-stemmed cultivar (‘AC Eatonia’) increased yield by an average of 16% (0.4 mg ha-1) and increased the grade of wheat by one unit at the two most heavily infested site-years. Planting a 1:1 blend of AC Eatonia and AC Barrie increased yield by an average of 11%, whereas planting 20- or 40-m plot margins to AC Eatonia increased yield by an average of 8%. High wheat stem sawfly pressure limited the effectiveness of using resistant cultivars in field margins because plants were often infested beyond the plot margin, with uniform infestation down the length of the plots at the two most heavily infested site-years. The effectiveness of AC Eatonia to reduce wheat stem sawfly survivorship was modest in this study, probably due to weather-related factors influencing pith expression and to the high abundance of wheat stem sawfly. Greater benefits from planting field margins to resistant cultivars or planting a blend of resistant and susceptible cultivars might be achievable under lower wheat stem sawfly pressure.
Axis I psychiatric comorbidity and its relationship to historical illness variables in 288 patients with bipolar disorder.

OBJECTIVE Bipolar disorder often co-occurs with other axis I disorders, but little is known about the relationships between the clinical features of bipolar illness and these comorbid conditions. Therefore, the authors assessed comorbid lifetime and current axis I disorders in 288 patients with bipolar disorder and the relationships of these comorbid disorders to selected demographic and historical illness variables. METHOD They evaluated 288 outpatients with bipolar I or II disorder, using structured diagnostic interviews and clinician-administered and self-rated questionnaires to determine the diagnosis of bipolar disorder, comorbid axis I disorder diagnoses, and demographic and historical illness characteristics. RESULTS One hundred eighty-seven (65%) of the patients with bipolar disorder also met DSM-IV criteria for at least one comorbid lifetime axis I disorder. More patients had comorbid anxiety disorders (N=78, 42%) and substance use disorders (N=78, 42%) than had eating disorders (N=9, 5%). There were no differences in comorbidity between patients with bipolar I and bipolar II disorder. Both lifetime axis I comorbidity and current axis I comorbidity were associated with earlier age at onset of affective symptoms and syndromal bipolar disorder. Current axis I comorbidity was associated with a history of development of both cycle acceleration and more severe episodes over time. CONCLUSIONS Patients with bipolar disorder often have comorbid anxiety, substance use, and, to a lesser extent, eating disorders. Moreover, axis I comorbidity, especially current comorbidity, may be associated with an earlier age at onset and worsening course of bipolar illness. Further research into the prognostic and treatment response implications of axis I comorbidity in bipolar disorder is important and is in progress.
Re-evaluating the use of the L1 in the L2 classroom: students vs. teachers

Abstract The present paper examines the effects of the monolingual and the bilingual approach in the second language (L2) classroom. The outcomes of two Likert type questionnaires and classroom observations have been employed to explore teachers’ and learners’ opinions and actions in order to evaluate how the use of the native language (L1) and the L2 is used by both learners and practitioners. Data analysis shows that there is a place for both languages and, when used in a balanced manner, they can comprise a positive cognitive effect due to the fact that the language learner actively draws in interlanguage development. The present study offers clear direction for further research as there is an evident lack of knowledge of principles of L1 use in similar EFL contexts.
Repair of the degenerate retina by photoreceptor transplantation

Despite different aetiologies, age-related macular degeneration and most inherited retinal disorders culminate in the same final common pathway, the loss of photoreceptors. There are few treatments and none reverse the loss of vision. Photoreceptor replacement by transplantation is proposed as a broad treatment strategy applicable to all degenerations. Recently, we demonstrated restoration of vision following rod-photoreceptor transplantation into a mouse model of stationary night-blindness, raising the critical question of whether photoreceptor replacement is equally effective in different types and stages of degeneration. We present a comprehensive assessment of rod-photoreceptor transplantation across six murine models of inherited photoreceptor degeneration. Transplantation is feasible in all models examined but disease type has a major impact on outcome, as assessed both by the morphology and number of integrated rod-photoreceptors. Integration can increase (Prph2+/Δ307), decrease (Crb1rd8/rd8, Gnat1−/−, Rho−/−), or remain constant (PDE6βrd1/rd1, Prph2rd2/rd2) with disease progression, depending upon the gene defect, with no correlation with severity. Robust integration is possible even in late-stage disease. Glial scarring and outer limiting membrane integrity, features that change with degeneration, significantly affect transplanted photoreceptor integration. Combined breakdown of these barriers markedly increases integration in a model with an intact outer limiting membrane, strong gliotic response, and otherwise poor transplantation outcome (Rho−/−), leading to an eightfold increase in integration and restoration of visual function. Thus, it is possible to achieve robust integration across a broad range of inherited retinopathies. Moreover, transplantation outcome can be improved by administering appropriate, tailored manipulations of the recipient environment.
Lower limb vascular conductance and resting popliteal blood flow during head‐up and head‐down postural challenges

This study hypothesized that central and local reflex mechanisms affecting vascular conductance (VC) through the popliteal artery compensated for the reduction in muscle perfusion pressure (MPP) to maintain popliteal blood flow (PBF) during head‐down tilt (35˚ HDT), but not in head‐up tilt (45˚ HUT). Resting measurements were made on 15 healthy men in prone position to facilitate the access to the popliteal artery, on two separate days in random order during horizontal (HOR), HDT or HUT. In each body position, the body was supported, and the ankles were maintained in relaxed state so that there was no muscle tension, as with normal standing. Popliteal blood flow velocity and popliteal arterial diameter were measured by ultrasound, and PBF was calculated. MPP was corrected to mid‐calf from measured finger cuff pressure, and VC was estimated by dividing PBF by MPP. The MPP in HDT (48 ± 2 mmHg) was ~100mmHg less than in HUT (145 ± 2 mmHg). PBF was similar between HOR (51 ± 18 ml min−1) and HDT (47 ± 13 ml min−1), but was lower in HUT (30 ± 9 ml min−1). VC was different between HDT (1·0 ± 0·3 ml min−1 mmHg−1), HOR (0·6 ± 0·2 ml min−1 mmHg−1) and HUT (0·2 ± 0·1 ml min−1 mmHg−1). In conclusion, the interactions of central and local regulatory mechanisms resulted in a disproportionate reduction of VC during HUT lowering PBF even though MPP was higher, while in HDT, increased VC contributed to maintain PBF at the same level as the HOR control condition.
Commissural Gain Control Enhances the Midbrain Representation of Sound Location

Accurate localization of sound sources is essential for survival behavior in many species. The inferior colliculi (ICs) are the first point in the auditory pathway where cues used to locate sounds, ie, interaural time differences (ITDs), interaural level differences (ILDs), and pinna spectral cues, are all represented in the same location. These cues are first extracted separately on each side of the midline in brainstem nuclei that project to the ICs. Because of this segregation, each IC predominantly represents stimuli in the contralateral hemifield. We tested the hypothesis that commissural connections between the ICs mediate gain control that enhances sound localization acuity. We recorded IC neurons sensitive to either ITDs or ILDs in anesthetized guinea pig, before, during, and following recovery from deactivation of the contralateral IC by cryoloop cooling or microdialysis of procaine. During deactivation, responses were rescaled by divisive gain change and additive shifts, which reduced the dynamic range of ITD and ILD response functions and the ability of neurons to signal changes in sound location. These data suggest that each IC exerts multiplicative gain control and subtractive shifts over the other IC that enhances the neural representation of sound location. Furthermore, this gain control operates in a similar manner on both ITD- and ILD-sensitive neurons, suggesting a shared mechanism operates across localization cues. Our findings reveal a novel dependence of sound localization on commissural processing. SIGNIFICANCE STATEMENT Sound localization, a fundamental process in hearing, is dependent on bilateral computations in the brainstem. How this information is transmitted from the brainstem to the auditory cortex, through several stages of processing, without loss of signal fidelity, is not clear. We show that the ability of neurons in the auditory midbrain to encode azimuthal sound location is dependent on gain control mediated by the commissure of the inferior colliculi. This finding demonstrates that commissural processing between homologous auditory nuclei, on either side of the midline, enhances the precision of sound localization.
Prognostic Applications of Gene Expression Signatures in Breast Cancer

Analysis by DNA microarrays has led to the identification of molecular subtypes of breast carcinomas that show a distinct expression profile. Several studies have demonstrated that this ‘intrinsic subtype’ classification has a strong prognostic value. In addition, gene expression profiling techniques have been used to identify gene signatures that could be associated with the outcome of breast cancer patients. Several different genomic tests have been shown to better define the prognosis of early-stage breast cancer patients as compared with conventional clinical and pathological characteristics of the tumors, and some assays are already commercially available. However, it must be emphasized that the prognostic power of these genetic classifiers has not been confirmed yet in prospective trials. Genetic signatures that might predict the activity of specific chemotherapy agents have also been developed by using gene expression profiling techniques. The same approach has been used to identify gene signatures associated with the activation of oncogenic pathways that might represent targets for molecular therapy of breast cancer. By using these approaches, gene expression techniques might significantly improve our ability to predict the risk of recurrence and to tailor the treatment for each individual breast cancer patient.
The Cost of Financial Flexibility: Evidence from Share Repurchases

Over the last two decades, share repurchases have emerged as the dominant payout channel, offering a more flexible means of returning excess cash to investors. However, little is known about the costs associated with payout-related financial flexibility. Using a unique identification strategy, we document a significant cost. We find that actual repurchase investments underperform hypothetical investments that mechanically smooth repurchase dollars through time by approximately two percentage points per year on average. This cost of financial flexibility is correlated with earnings management, managerial entrenchment, and less institutional monitoring.
Evaluation of selenium status in pure bred duroc sows and their progeny.

OBJECTIVES The aim of this trial was to determine selenium status in pure bred duroc sows and their progeny and to compare it to Czech Large White x Landrace breed. DESIGN The pregnant duroc sows (n=12) and pregnant Czech Large White x Landrace sows (n=12) were fed identical diets supplemented with sodium selenite. RESULTS During lactation significantly higher serum Se concentrations (p<0.001) were found in duroc piglets. Also significantly higher serum GSH-Px activities (p<0.001) were found in duroc sows and piglets. No differences in concentrations of Se in colostrums and milk were found between the two breeds. CONCLUSION Our findings support the hypothesis that there may be breed differences in indices of selenium status in pigs.
Visualisation of Linked Data - Reprise

Linked Data promises to serve as a disruptor of traditional approaches to data management and use, promoting the push from the traditional Web of documents to a Web of data. The ability for data consumers to adopt a follow your nose approach, traversing links defined within a dataset or across independently-curated datasets, is an essential feature of this new Web of Data, enabling richer knowledge retrieval thanks to synthesis across multiple sources of, and views on, interrelated datasets. But for the Web of Data to be successful, we must design novel ways of interacting with the corresponding very large amounts of complex, interlinked, multi-dimensional data throughout its management cycle. The design of user interfaces for Linked Data, and more specifically interfaces that represent the data visually, play a central role in this respect. Contributions to this special issue on Linked Data visualisation investigate different approaches to harnessing visualisation as a tool for exploratory discovery and basic-to-advanced analysis. The papers in this volume illustrate the design and construction of intuitive means for end-users to obtain new insight and gather more knowledge, as they follow links defined across datasets over the Web of Data.
Severe dengue with acute abdomen- a diagnosis dilemma

Dengue is an emerging mosquito-borne viral infection transmitted between humans by Aedes spp . The rapid growth of dengue cases globally created a public health challenge in managing it. Since the dengue is a viral disease, a patient usually presented with non- specific clinical features with no definite drug or vaccine proven to treat it. Hence, a simple diagnostic tool for the early phase of the illness, early supportive treatment and efficient vector control measures are the essential elements in a holistic approach to managing a dengue infection. However, with a non-specific presentation of the disease and high variability of rapid diagnostic test sensitivity and specificity, the diagnosis is more challenging. The failure of detecting dengue infection, especially the severe variety may lead to a grave consequence. This case illustrates how a non- specific presentation with false negative diagnostic test result leads to unnecessary surgical intervention in a severe dengue patient. Keywords : acute abdominal pain; dengue serology; severe dengue; Cite as: Azizul Fadzli Wan Jusoh, Nik Ahmad Shaiffudin Nik Him, Abdul Hafiz Shaharudin, et al (2019) Severe dengue with acute abdomen- a diagnosis dilemma. Asian Journal of Medicine & Biomedicine. Vol 3 (1): Date received: February 13 th , 2019 Date accepted: April 15 th , 2019
Potential prognostic long non-coding RNA identification and their validation in predicting survival of patients with multiple myeloma

Multiple myeloma, a typical hematological malignancy, is characterized by malignant proliferation of plasma cells. This study was to identify differently expressed long non-coding RNAs to predict the survival of patients with multiple myeloma efficiently. Gene expressing profiles of diagnosed patients with multiple myeloma, GSE24080 (559 samples) and GSE57317 (55 samples), were downloaded from Gene Expression Omnibus database. After processing, survival-related long non-coding RNAs were identified by Cox regression analysis. The prognosis of multiple myeloma patients with differently expressed long non-coding RNAs was predicted by Kaplan–Meier analysis. Meanwhile, stratified analysis was performed based on the concentrations of serum beta 2-microglobulin (S-beta 2m), albumin, and lactate dehydrogenase of multiple myeloma patients. Gene set enrichment analysis was performed to further explore the functions of identified long non-coding RNAs. A total of 176 long non-coding RNAs significantly related to the survival of multiple myeloma patients (p < 0.05) were identified. In dataset GSE24080 and GSE57317, there were 558 and 55 patients being clustered into two groups with significant differences, respectively. Stratified analysis indicated that prediction of the prognoses with these long non-coding RNAs was independent from other clinical phenotype of multiple myeloma. Gene set enrichment analysis–identified pathways of cell cycle, focal adhesion, and G2-M checkpoint were associated with these long non-coding RNAs. A total of 176 long non-coding RNAs, especially RP1-286D6.1, AC008875.2, MTMR9L, AC069360.2, and AL512791.1, were potential biomarkers to evaluate the prognosis of multiple myeloma patients. These long non-coding RNAs participated indispensably in many pathways associated to the development of multiple myeloma; however, the molecular mechanisms need to be further studied.
HIV envelope induces a cascade of cell signals in non-proliferating target cells that favor virus replication

Certain HIV-encoded proteins modify host-cell gene expression in a manner that facilitates viral replication. These activities may contribute to low-level viral replication in nonproliferating cells. Through the use of oligonucleotide microarrays and high-throughput Western blotting we demonstrate that one of these proteins, gp120, induces the expression of cytokines, chemokines, kinases, and transcription factors associated with antigen-specific T cell activation in the absence of cellular proliferation. Examination of transcriptional changes induced by gp120 in freshly isolated peripheral blood mononuclear cells and monocyte-derived-macrophages reveals a broad and complex transcriptional program conducive to productive infection with HIV. Observations include the induction of nuclear factor of activated T cells, components of the RNA polymerase II complex including TFII D, proteins localized to the plasma membrane, including several syntaxins, and members of the Rho protein family, including Cdc 42. These observations provide evidence that envelope-mediated signaling contributes to the productive infection of HIV in suboptimally activated T cells.
Regional striatal DOPA transport and decarboxylase activity in Parkinson's disease.

METHODS We measured blood-brain barrier transport and decarboxylation of 6-[18F]fluoro-L-DOPA (FDOPA) using PET in patients with Parkinson's disease (n = 7, 57 +/- 7 yr) and age-matched control subjects (n = 7, 60 +/- 6 yr). To visually present regional changes of FDOPA uptake in Parkinson's disease, we introduced maps of FDOPA uptake relative to occipital cortex, averaged across control subjects and Parkinson's disease patients in an MRI-based stereotaxic coordinate space. RESULTS There was no significant changes in the blood-to-brain transport of FDOPA (KD1) in Parkinson's disease. The KD1 values of the head of caudate were lower than those of putamen in both normal subjects and Parkinson's disease patients. In Parkinson's disease, the activity of L-DOPA decarboxylase (DDC) was differentially reduced in subdivisions of striatum. The residual DDC activity was 63% of the control value in the head of caudate nucleus, 54% in the anterior putamen and 39% in the posterior putamen. The DDC activity in frontal and occipital cortices remained unchanged by the disease. Subtraction of averaged FDOPA uptake maps (control minus Parkinson's disease) visualized a spatial pattern of pathological changes in FDOPA uptake common to Parkinson's disease patients. CONCLUSION The striatal blood-to-brain transport of FDOPA remained unchanged while the DDC activity was differentially reduced within the striatum in Parkinson's disease. We found the FDOPA uptake maps useful in identifying altered patterns of FDOPA metabolism common in Parkinson's disease.