Miracle-dz/UniFAR-FTUs huggingface dataset:
Pupillometry in Mice: Sex and Strain-Dependent Phenotypes of Pupillary Functioning

Purpose. Pupillometry is used as a phenotyping assay for investigating pupil responses in normal and genetically engineered mice. The mice used most often in contemporary ophthalmic research have not been adequately investigated by pupillometry. Methods. An infrared-video camera was used to assess pupillary functioning under standard dark-adapted conditions (30 min acclimatization in a ganzfeld chamber) and 30 min after the installation of either 10 &mgr;l tropicamide (1.0%) or 10 &mgr;l pilocarpine (5.0%) into the conjunctival cul-de-sac. The experiment was self-controlled using a repeated-measures analysis of variance to analyze 60 mice (30 males, 24 weeks of age) from three strains (C57BL/6, 129SvJ, and F1 hybrid). Results. The dark-adapted pupillary diameter of mice ranged from 2.3 mm (SD = 0.14) in female C57BL/6 to 2.9 mm (SD = 0.05) in male 129SvJ. Under dark-adapted conditions, all mice examined were sexual dimorphic (F = 19.5, dF = 2, 119, p < 0.001) and strain-dependent differences were observed between male C57BL/6-129SvJ and C57BL/6-F1 and female C57BL/6-F1 (F = 82.32, dF = 1, 119, p < 0.001). The mean pupillary diameter 30 min after the application of tropicamide ranged from 2.5 mm (SD = 0.16) in female F1 to 3.0 mm (SD = 0.13) in male C57BL/6. Tropicamide produced a sexual dimorphism in mydriasis for all mice (F = 56.30, dF = 1, 59, p < 0.001); however, strain-dependent differences were not observed (F = 1.31, dF = 2, 59, p < 0.280). The mean pupillary diameter 30 min after the application of pilocarpine ranged from 2.6 mm (SD = 0.28) in female C57BL/6 to 3.2 mm in both male F1 (SD = 0.22) and 129SvJ (SD = 0.04). Pilocarpine produced a sexual dimorphism in mydriasis for 129SvJ and F1 (F = 106.70, dF = 1, 59, p < 0.001) and strain-dependent differences were observed between female C57BL/6-F1 (F = 17.25, dF = 2, 59, p < 0.001). Conclusions. The experiment demonstrates that mice respond idiosyncratically in their pupillary response under standard dark-adapted conditions and to either tropicamide or pilocarpine depending on strain and/or sex of the mouse. The characteristic responses observed are likely due to subtle differences in the genetic expression of phenotype.
Evaluation of the added value of LAMEPS in contrast to global EPS

Um eine genauere Vorhersage von signifikanten Wetterereignissen zu ermoglichen, wurde das regionale Ensemblevorhersagesystem ALADIN-LAEF (Aire Limitee Adaption Dynamique Developpment International - Limited Area Forecasting) entwickelt. Dieses kann aufgrund seiner deutlich hoheren Auflosung im Vergleich zu globalen Ensemblevorhersagesystemen, lokale Gegebenheiten wie Gebirge besser berucksichtigen. Aus diesem Grund nimmt man an, dass die Qualitat der Prognosen klein skaligerWetterphanomene durch den Einsatz von regionalen Ensemblevorhersagesystemen steigt. Um diese Annahme zu uberprufen, wurden die Prognosen von ALADIN-LAEF und dem globalen ECMWF-EPS (European Centre for Medium Range Weather Forecasts - Ensemble Prediction System) verifiziert und anschlieSSend verglichen. Ziel der Verifikation war es, potentielle Vorteile des regionalen Ensemblevorhersagesystems ALADIN-LAEF herauszufiltern und Nachteile zu lokalisieren. Fur die Verifikation wurden samtliche Prognosen im Zeitraum vom 20. Juni 2007 bis 20. August 2007 uberpruft. Zur Beurteilung der Prognosequalitat wurde eine breite Auswahl an VerifikationsmaSSen berechnet. Dazu zahlen beispielsweise Bias, Brier Score oder auch CRPS. Der Vergleich der Ergebnisse der Bodenfelder zeigt, dass ALADIN-LAEF bessereWindund Niederschlagsprognosen erzeugt. Im Gegensatz dazu produziert das ECMWFEPS deutlich bessere Temperatur- und Bodendruckprognosen. Dabei sollte erwahnt werden, dass vor allem die Qualitat der Temperaturprognosen von ALADIN-LAEF durch Kalibration deutlich erhoht werden konnte. Die Verifikation der Hohenfelder zeigt, dass das ECMWF-EPS deutlich bessereWind-, Feuchte- und 500 hPa Geopotential- Prognosen erzeugt. Dagegen produziert ALADIN-LAEF bessere Temperaturprognosen fur das 500 hPa-Feld. Dabei muss erwahnt werden, dass die 500 hPa Temperaturprognosen des ECMWF-EPS einen deutlichen Bias aufweisen. Da systematische Fehler einen betrachtlichen Einfluss auf die Qualitat der Prognosen haben, sind Korrekturmethoden von besonderem Interesse. Kalibrationsmethoden fur die 2m Temperaturprognosen wurden an der ZAMG bereits erfolgreich getestet. Fazit dieser Untersuchung ist, dass der Mehrwert von ALADIN-LAEF im Vergleich zum ECWMF-EPS stark von dem betrachteten Parameter abhangt.
Intimate Partner Victimization Among College Students With and Without Disabilities

Prior research indicates that both college students and individuals with disabilities are at an increased risk of experiencing intimate partner victimization (IPV). However, little is known about IPV risk and its relationship to emotional well-being among the intersection of these two populations. Utilizing a sample of approximately 20,000 college students from the American College Health Association’s (ACHA) National College Health Assessment II (NCHA II), this study focuses on this overlooked intersection by examining IPV among college students with disabilities. Multivariate binary logistic regression models were used to estimate the relationship among disability, IPV, and emotional well-being. College students with disabilities were approximately twice as likely to experience IPV than their counterparts without disabilities. Students with mental disabilities and multiple disability types were found to have the greatest likelihood of experiencing IPV. Victims with disabilities were more likely than victims without disabilities to report experiencing depression symptoms, self-harm behavior, and stress. Recommendations for reducing and preventing IPV among a college student population are discussed.
Exploring the experiences of older Chinese adults with comorbidities including diabetes: surmounting these challenges in order to live a normal life

Background Many people with diabetes have comorbidities, even multimorbidities, which have a far-reaching impact on the older adults, their family, and society. However, little is known of the experience of older adults living with comorbidities that include diabetes. Aim The aim of this study was to explore the experience of older adults living with comorbidities including diabetes. Methods A qualitative approach was employed. Data were collected from a selected field of 12 patients with diabetes mellitus in a medical center in northern Taiwan. The data were analyzed by Colaizzi’s phenomenological methodology, and four criteria of Lincoln and Guba were used to evaluate the rigor of the study. Results The following 5 themes and 14 subthemes were derived: 1) expecting to heal or reduce the symptoms of the disease (trying to alleviate the distress of symptoms and trusting in health practitioners combining the use of Chinese and Western medicines); 2) comparing complex medical treatments (differences in physician practices and presentation, conditionally adhering to medical treatment, and partnering with medical professionals); 3) inconsistent information (inconsistent health information and inconsistent medical advice); 4) impacting on daily life (activities are limited and hobbies cannot be maintained and psychological distress); and 5) weighing the pros and cons (taking the initiative to deal with issues, limiting activity, adjusting mental outlook and pace of life, developing strategies for individual health regimens, and seeking support). Surmounting these challenges in order to live a normal life was explored. Conclusion This study found that the experience of older adults living with comorbidities including diabetes was similar to that of a single disease, but the extent was greater than a single disease. The biggest difference is that the elderly think that their most serious problem is not diabetes, but rather, the comorbidities causing life limitations. Therefore, compared to the elderly suffering from a single disease of diabetes, medical professionals not only care about physiological data of the elderly but also pay attention to the impact of comorbidity on their lives.
The Composition of Stigmatic Exudate from Lilium longiflorum: Labeling Studies with Myo-inositol, d-Glucose, and l-Proline.

Stigmatic exudate, a secretion product recovered from the upper surface of Lilium longiflorum pistils, has been examined. Over 99% of the exudate is accounted for as water, carbohydrate, and protein. Exclusive of water, 95% is a high molecular weight, protein-containing polysaccharide composed of galactose, arabinose, rhamnose, glucuronic acid, and galacturonic acid.Detached pistils supplied with myo-inositol-U-(14)C, myo-inositol-2-(3)H, d-glucose-1-(14)C, or l-proline-U-(14)C produce labeled stigmatic exudate. When myo-inositol is supplied, the exudate is rich in labeled arabinose and uronic acids, but some label also recycles through the hexose phosphate pool of secreting cells, causing label to appear in galactose and rhamnose residues. When glucose is provided, galactose is the major constituent labeled but all of the other carbohydrate constituents are also labeled. Proline produces a pattern very similar to that obtained with glucose.Stigmatic exudate also contains a small amount of low molecular weight carbohydrate. If myo-inositol is used to label exudate, free labeled myo-inositol cannot be detected in the low molecular weight fraction until it has been subjected to acid hydrolysis. Similarly, if d-glucose is the source of label, free labeled glucose is found in the low molecular weight fraction only after acid hydrolysis.
High trans but not saturated fat beverage causes an acute reduction in postprandial vascular endothelial function but not arterial stiffness in humans

A diet high in trans-fatty acids (TFAs) is associated with a higher risk of cardiovascular disease (CVD) than a diet high in saturated fatty acids (SFAs), but the mechanisms remain unclear. We hypothesized that a beverage high in TFAs would cause a larger reduction in postprandial endothelial function and an increase in arterial stiffness, in part from greater reductions in insulin sensitivity, compared with a beverage high in SFAs. Eleven healthy adults (aged 47±5 years) ingested a warm test beverage (520 kcal, 56 g total fat, 5 g carbohydrate, 1 g protein) high in either TFAs or SFAs in a randomized cross-over study. Ingestion of the beverage high in TFAs (p<0.01) but not high in SFAs (p=0.49) decreased endothelial function (brachial artery flow-mediated dilation, mmΔ) at 3–4 hours (p<0.01 for time; p=0.034 for interaction), but did not alter aortic stiffness or carotid β-stiffness. The homeostasis model of insulin resistance (interaction p=0.062) tended to decrease after SFAs but not TFAs. A beverage high in TFAs but not SFAs results in a postprandial reduction in endothelial function and a trend for decreased insulin sensitivity, potentially explaining the higher risk of CVD with a diet high in TFAs.
Work-related back pain in nurses.

This summary draws together the findings form over 80 studies published over three decades. The studies reviewed are categorized into three groups: (a) epidemiological; (b) 'testing out'; and (c) exploratory. There has been agreement on a number of points, in particular that nursing is among the high risk occupations with respect to low back problems, with a point prevalence of approximately 17%, an annual (period) prevalence of 40-50% and a lifetime prevalence of 35-80%. When considering the contributory factors there is some divergence, but one of the popular notions is generally proven, that more frequent patient handling appears to correlate with increased incidence of low back pain. However, the traditional approach of training in lifting and handling techniques alone has been shown to be of little, or no, long-term benefit and the value of ergonomics remains to be seen. Much work has also been done by taking aspects of nursing work into the laboratory, using experimental studies which have mostly focused on specific sub-tasks (of the generic task of patient handling), looking at specific transfers and procedures (e.g. bed to chair) or transfer techniques ('stoop versus squat'). Although a level of quantification can be made about the different techniques, it is questionable whether this is of any practical use, especially when considering the wide variation of loads encountered during manual handling of patients. The limitations of using quantitative methodologies is revealed in the very small number of exploratory studies. All of the studies cited in this review used methodologies based in the positivist paradigm. There does not appear to be any published work using participative or interview methods to obtain qualitative data which might identify contributory factors in the onset of occupational low back pain in nursing staff.
Endothelin-induced biphasic response of lactotrophs cultured under different conditions.

Endothelin (ET), a recently discovered vasoconstrictor peptide, is widely distributed in different tissues including brain and pituitary. Although evidence regarding the role of ET in neuroendocrine processes is still fragmentary, it appears that the release of several anterior pituitary (AP) hormones can be modulated by peptides of the ET family. In the present study, we compared the effects of ET-1 and ET-3 on the release of PRL from AP cells cultured in serum-free (SFM) or serum-containing (SCM) medium. AP obtained from adult male rats were enzymatically dispersed, and the cells were plated in either SFM or SCM. After 4 days, cells were incubated with ET-1 or ET-3 for designated periods of time, and PRL levels in the incubation media were measured by RIA. When AP cells maintained in SCM were exposed to ET for 20 min, both peptides inhibited PRL release, with ET-1 being a more potent inhibitor than ET-3. In contrast, a biphasic response was observed in cultures grown in SFM: low concentrations of ET peptides inhibited the secretion of PRL, while high concentrations caused a significant stimulation. Further characterization of the effects of ET-1 revealed that the stimulatory phase was relatively short (15-30 min) and was followed by inhibition of PRL release. The addition of either horse serum or fetal bovine serum to SFM on the day of plating resulted in a dose-dependent reduction in the ET-induced stimulation of lactotrophs. These findings indicate that the presence of serum in culture medium alters the expression of cell properties underlying inhibitory and stimulatory responses to ET in terms of PRL secretion. (ABSTRACT TRUNCATED AT 250 WORDS)
Apoptosis induced by troglitazone is both peroxisome proliferator‐activated receptor‐γ‐ and ERK‐dependent in human non‐small lung cancer cells

The role of the peroxisome proliferator‐activated receptor‐gamma (PPARγ) in cell differentiation, cell‐cycle arrest, and apoptosis has attracted increasing attention. We have recently demonstrated that PPARγ ligands‐troglitazone (TGZ) induced apoptosis in lung cancer cells. In this report, we further studied the role of ERK1/2 in lung cancer cells treated by TGZ. The result demonstrated that TGZ induced PPARγ and ERK1/2 accumulation in the nucleus, in which the co‐localization of both proteins was found. The activation of ERK1/2 resulted in apoptosis via a mitochondrial pathway. Both PPARγ siRNA and U0126, a specific inhibitor of ERK1/2, were able to block these effects of TGZ, suggesting that apoptosis induced by TGZ was PPARγ and ERK1/2 dependent. Inhibition of ERK1/2 by U0126 also led to a significant decrease in the level of PPARγ, indicating a positive cross‐talk between PPARγ and ERK1/2 or an auto‐regulatory feedback mechanism to amplify the effect of ERK1/2 on cell growth arrest and apoptosis. In addition to ERK1/2, TGZ also activated Akt. Interestingly, inhibition of ERK1/2 prevented the activation of Akt whereas the suppression of Akt had no effect on ERK1/2, suggesting that Akt was not necessary for TGZ‐PPARγ‐ERK pathway. However, the inhibition of Akt promoted the release of cytochrome c, suggesting the activation of Akt may have a negative effect on apoptosis induced by TGZ. In conclusion, our study has demonstrated that TGZ, a synthetic PPARγ ligand, induced apoptosis in NCI‐H23 lung cancer cells via a mitochondrial pathway and this pathway was PPARγ and ERK1/2 dependent. J. Cell. Physiol. 209: 428–438, 2006. © 2006 Wiley‐Liss, Inc.
Interleukin-1 Receptor Antagonist Gene Polymorphism, Vaginal Interleukin-1 Receptor Antagonist Concentrations, and Vaginal Ureaplasma urealyticum Colonization in Pregnant Women

ABSTRACT Ureaplasma urealyticum is the microorganism most frequently isolated from amniotic fluids of women in preterm labor. The relationship between vaginal colonization with U. urealyticum, vaginal interleukin-1 receptor antagonist (IL-1ra) levels, and the IL-1ra genotype in pregnant women was examined. Vaginal specimens, obtained with a cotton swab from 207 women in their first trimester of pregnancy, were tested for IL-1ra concentrations by enzyme-linked immunosorbent assay and for U. urealyticum and IL-1ra genotypes by PCR. U. urealyticum was detected in 85 (41.1%) women. The median IL-1ra level was 450 ng/ml in women positive for U. urealyticum, as opposed to 225 ng/ml in women negative for this microorganism (P < 0.0001). Sixty-two percent of the 16 women who were homozygous for allele 2 of the IL-1ra gene (IL-1RN*2) were colonized with U. urealyticum, as opposed to 47% of the 49 women who were IL-1RN*1/IL-1RN*2 heterozygotes and 34% of the 133 women who were IL-1RN*1 homozygotes (P < 0.05). Median IL-1ra levels were 750 ng/ml in IL-1RN*2 homozygotes, 300 ng/ml in IL-1RN*1/IL-1RN*2 heterozygotes, and 250 ng/ml in IL-1RN*1 homozygotes (P = 0.02). The vast majority of subjects had an uneventful pregnancy and delivered a healthy infant at term. The IL-1ra genotype or U. urealyticum colonization was unrelated to birth weight. Pregnant women who are colonized with U. urealyticum during the first trimester have elevated vaginal IL-1ra concentrations and a higher prevalence of the IL-1RN*2 homozygote genotype than do noncolonized women.
A one‐week quadruple eradication regimen for Helicobacter pylori in routine clinical practice

Background: The addition of omeprazole to 1 week of standard triple therapy (tripotassium dicitrato bismuthate, tetracycline and metronidazole) has given a 98% eradication rate in 54 patients at 4–6 weeks in a research setting. We report the result of a similar 1‐week regimen in 52 patients in routine clinical practice assessed at a mean of 8 months.
Transplantation for Type I Diabetes: Comparison of Vascularized Whole-Organ Pancreas With Isolated Pancreatic Islets

Objective:We sought to compare the efficacy, risks, and costs of whole-organ pancreas transplantation (WOP) with the costs of isolated islet transplantation (IIT) in the treatment of patients with type I diabetes mellitus. Summary Background Data:A striking improvement has taken place in the results of IIT with regard to attaining normoglycemia and insulin independence of type I diabetic recipients. Theoretically, this minimally invasive therapy should replace WOP because its risks and expense should be less. To date, however, no systematic comparisons of these 2 options have been reported. Methods:We conducted a retrospective analysis of a consecutive series of WOP and IIT performed at the University of Pennsylvania between September 2001 and February 2004. We compared a variety of parameters, including patient and graft survival, degree and duration of glucose homeostasis, procedural and immunosuppressive complications, and resources utilization. Results:Both WOP and IIT proved highly successful at establishing insulin independence in type I diabetic patients. Whole-organ pancreas recipients experienced longer lengths of stay, more readmissions, and more complications, but they exhibited a more durable state of normoglycemia with greater insulin reserves. Achieving insulin independence by IIT proved surprisingly more expensive, despite shorter initial hospital and readmission stays. Conclusion:Despite recent improvement in the success of IIT, WOP provides a more reliable and durable restoration of normoglycemia. Although IIT was associated with less procedure-related morbidity and shorter hospital stays, we unexpectedly found IIT to be more costly than WOP. This was largely due to IIT requiring islets from multiple donors to gain insulin independence. Because donor pancreata that are unsuitable for WOP can often be used successfully for IIT, we suggest that as IIT evolves, it should continue to be evaluated as a complementary alternative to rather than as a replacement for the better-established method of WOP.
Restoration of Blood Flow to the Proximal Femoral Epiphysis in Unstable Slipped Capital Femoral Epiphysis by Modified Dunn Procedure: A Preliminary Angiographic and Intracranial Pressure Monitoring Study

Background:The major complication of unstable slipped capital femoral epiphysis (SCFE) is avascular necrosis (AVN) of the femoral head. The purpose of this study was to document by angiography the preoperative and postoperative perfusion to the proximal femoral epiphysis following an unstable SCFE. A specific aim was to determine whether blood flow could be restored. A secondary aim was to determine the efficacy of an intracranial pressure (ICP) monitor to assess blood flow within the femoral head intraoperatively. Methods:Nine patients with an unstable SCFE underwent superselective angiogram of the medial circumflex femoral artery preoperatively, followed by operative fixation with an open reduction using a modified Dunn approach. Femoral head blood flow was evaluated with an ICP monitor. Angiography was repeated postoperatively. Patients were followed radiographically to assess for AVN. Results:Follow-up averaged 22 months. Six patients did not have arterial flow to the femoral head on the preoperative angiogram. Flow was restored postoperatively on angiogram in 4 of the 6 patients. Two patients developed AVN. One had no flow to the femoral head preoperatively or postoperatively on angiogram and complete tearing of the periosteum was noted. In 1 patient, there was no ICP waveform after the initial reduction. After removing more callous and repeating reduction, the waveform returned. Of the 2 patients with AVN, 1 had an ICP waveform after reduction. Conclusions:This study documents that some patients with unstable SCFE present with reduced femoral head blood supply due to SCFE. It also demonstrates blood flow restoration in 4 patients by angiogram and 5 by ICP monitor after surgical treatment. No patient immediately lost blood flow due to surgery. ICP monitor is a safe intraoperative tool for real-time assessment of femoral head blood flow during open reduction of unstable SCFE. Presence of flow by ICP is not a guarantee that AVN will not develop, but absence of flow was predictive of AVN. Level of Evidence:Therapeutic level I—prognostic. See Instructions for Authors for a complete description of levels of evidence.
IgG gene expression and its possible significance in prostate cancers

In spite of recent advances in treatment strategies, prostate cancer (PCa) remains the second leading cause of cancer death in men with its genetic and biologic behaviors still poorly understood. Recently, accumulating evidence indicates that cancer cells, as well as some normal cells can secret IgG. This study was designed to evaluate IgG gene expression and its possible significance in PCa tissue samples and cell lines.