Miracle-dz/UniFAR-FTUs huggingface dataset:
Sustained off‐treatment viral control is associated with high hepatitis B surface antigen seroclearance rates in Caucasian patients with nucleos(t)ide analogue–induced HBeAg seroconversion

Nucleos(t)ide Analogue withdrawal has been described to result in significant Hepatitis B surface antigen (HBsAg) loss rates in patients with long-term viral suppression and negative HBeAg at the start of treatment. In these patients off-treatment HBsAg loss is often preceded by viral rebound with concomitant Alanine Aminotransferase flares. In the present study, we investigated off-treatment HBsAg loss in a multicentric, international observational cohort of patients with Nucleos(t)ide Analogue induced HBeAg seroconversion. Ninety eight mono-infected, predominantly male (74.4%) chronic hepatitis B patients of mixed ethnicity (43.9% Asians, 49.0% Caucasians) who stopped treatment at a median of 11.4 (6.1-18.0) months after HBeAg seroconversion were included. A total of 16 patients experienced Hepatitis B surface Antigen loss during a median follow-up of 42.8 (22.7-83.2) months after treatment cessation: 14 off-treatment and 2 after retreatment initiation with subsequent long-term viral suppression. All patients with off-treatment HBsAg loss showed persistently low ALT (<1.5xULN) and HBV DNA (<2000 IU/mL) levels after HBeAg seroconversion. Persistent viral control was associated with significantly higher annual HBsAg seroclearance rates (8.4%) than relapse with (1.5%; p=0.008) or without (0.0%; p=0.009) subsequent retreatment. In addition, Caucasian patients with sustained off-treatment viral control had >6-fold higher annual HBsAg loss rates compared to non-Caucasian patients (14.8% vs 2.3% respectively; p=0.004). Persistent viral control after treatment cessation following NA induced HBeAg seroconversion was associated with high HBsAg loss rates in Caucasian patients.
Bidirectional LSTM with a Context Input Window for Named Entity Recognition in Tweets

Lately, with the increasing popularity of social media technologies, applying natural language processing for mining information in tweets has posed itself as a challenging task and has attracted significant research efforts. In contrast with the news text and others formal content, tweets pose a number of new challenges, due to their short and noisy nature. Thus, over the past decade, different Named Entity Recognition (NER) architectures have been proposed to solve this problem. However, most of them are based on handcrafted-features and restricted to a particular domain, which imposes a natural barrier to generalize over different contexts. In this sense, despite the long line of work in NER on formal domains, there are no studies in NER for tweets in Portuguese (despite 17.97 million monthly active users). To bridge this gap, we present a new gold-standard corpus of tweets annotated for Person, Location, and Organization (PLO). Additionally, we also perform multiple NER experiments using a variety of Long Short-Term Memory (LSTM) based models without resorting to any handcrafted rules. Our approach with a centered context input window of word embeddings yields 52.78 F1 score, 38.68% higher compared to a state of the art baseline system.
Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

Synthetic surfactants are becoming increasingly unpopular in many applications due to previously disregarded effects on biological systems and this has led to a new focus on replacing such products with biosurfactants that are biodegradable and produced from renewal resources. Microbially derived biosurfactants have been investigated in numerous studies in areas including: increasing feed digestibility in an agricultural context, improving seed protection and fertility, plant pathogen control, antimicrobial activity, antibiofilm activity, wound healing and dermatological care, improved oral cavity care, drug delivery systems and anticancer treatments. The development of the potential of biosurfactants has been hindered somewhat by the myriad of approaches taken in their investigations, the focus on pathogens as source species and the costs associated with large‐scale production. Here, we focus on various microbial sources of biosurfactants and the current trends in terms of agricultural and biomedical applications.
Human Cardiac-Mesenchymal Stem Cell-Like Cells, a Novel Cell Population with Therapeutic Potential

Cardiac stem/progenitors are being used in the clinic to treat patients with a range of cardiac pathologies. However, improvements in heart function following treatment have been reported to be variable, with some showing no response. This discrepancy in response remains unresolved. Mesenchymal stem cells (MSCs) have been highlighted as a regenerative tool as these cells display both immunomodulatory and proregenerative activities. The purpose of this study was to derive a cardiac MSC population to provide an alternative/support to current therapies. We derived human cardiac-mesenchymal stem cell-like cells (CMSCLC), so named as they share some MSC characteristics. However, CMSCLC lack the MSC trilineage differentiation capacity, being capable of only rare adipogenic differentiation and demonstrating low/no osteogenic or chondrogenic potential, a phenotype that may have advantages following transplantation. Furthermore, CMSCLC expressed low levels of p16, high levels of MHCI, and low levels of MHCII. A lack of senescent cells would also be advantageous for cells to be used therapeutically, as would the ability to modulate the immune response. Crucially, CMSCLC display a transcriptional profile that includes genes associated with cardioprotective/cardiobeneficial effects. CMSCLC are also secretory and multipotent, giving rise to cardiomyocytes and endothelial cells. Our findings support CMSCLC as a novel cell population suitable for use for transplantation.
Multi-Crop Green LAI Estimation with a New Simple Sentinel-2 LAI Index (SeLI)

The spatial quantification of green leaf area index (LAIgreen), the total green photosynthetically active leaf area per ground area, is a crucial biophysical variable for agroecosystem monitoring. The Sentinel-2 mission is with (1) a temporal resolution lower than a week, (2) a spatial resolution of up to 10 m, and (3) narrow bands in the red and red-edge region, a highly promising mission for agricultural monitoring. The aim of this work is to define an easy implementable LAIgreen index for the Sentinel-2 mission. Two large and independent multi-crop datasets of in situ collected LAIgreen measurements were used. Commonly used LAIgreen indices applied on the Sentinel-2 10 m × 10 m pixel resulted in a validation R2 lower than 0.6. By calculating all Sentinel-2 band combinations to identify high correlation and physical basis with LAIgreen, the new Sentinel-2 LAIgreen Index (SeLI) was defined. SeLI is a normalized index that uses the 705 nm and 865 nm centered bands, exploiting the red-edge region for low-saturating absorption sensitivity to photosynthetic vegetation. A R2 of 0.708 (root mean squared error (RMSE) = 0.67) and a R2 of 0.732 (RMSE = 0.69) were obtained with a linear fitting for the calibration and validation datasets, respectively, outperforming established indices. Sentinel-2 LAIgreen maps are presented.
Automatically extracting highlights for TV Baseball programs

In today's fast-paced world, while the number of channels of television programming available is increasing rapidly, the time available to watch them remains the same or is decreasing. Users desire the capability to watch the programs time-shifted (on-demand) and/or to watch just the highlights to save time. In this paper we explore how to provide for the latter capability, that is the ability to extract highlights automatically, so that viewing time can be reduced. We focus on the sport of baseball as our initial target—it is a very popular sport, the whole game is quite long, and the exciting portions are few. We focus on detecting highlights using audio-track features alone without relying on expensive-to-compute video-track features. We use a combination of generic sports features and baseball-specific features to obtain our results, but believe that may other sports offer the same opportunity and that the techniques presented here will apply to those sports. We present details on relative performance of various learning algorithms, and a probabilistic framework for combining multiple sources of information. We present results comparing output of our algorithms against human-selected highlights for a diverse collection of baseball games with very encouraging results.
The Impact of Financial Toxicity on Psychological Well-Being, Coping Self-Efficacy, and Cost-Coping Behaviors in Young Adults with Cancer.

Purpose: The increase in cost-sharing between patients and payers has resulted in financial toxicity in cancer patients, particularly among young adult (YA) patients and survivors (<40 years of age). This study explored financial toxicity and its impact on psychological well-being, self-efficacy for coping with cancer, and cost-coping behaviors among a sample of YA cancer patients and survivors. Methods: One hundred forty YAs completed an anonymous online survey. The Comprehensive Score for Financial Toxicity measured financial toxicity and the Cancer Behavior Inventory-Brief measured coping self-efficacy. The Patient Health Questionnaire-4 and items from the Impact of Cancer-Young Adult and the Cancer Needs Questionnaire-Young People assessed psychological well-being. A single item assessed cost-coping behaviors (i.e., skipping or delaying treatment because of its cost). Analyses included Pearson and Spearman correlation matrices and multivariate regression modeling. Results: Worse financial toxicity was associated with lower insurance satisfaction (r = 0.52, p < 0.001), higher levels of depressive and anxiety symptoms (r = -0.42, p < 0.001), greater worry (p < 0.001), and lower self-efficacy in coping with cancer (i.e., maintaining independence and a positive attitude, r = 0.41, p < 0.001; coping and stress management, r = 0.43, p < 0.001; and managing negative effect, r = 0.20, p = 0.02). In multivariate modeling, financial toxicity related to skipping or delaying treatment and greater anxiety and depression symptomology, controlling for relevant covariates. Conclusion: The findings suggest financial toxicity negatively impacts many facets of the YA cancer experience. There is a need to address the cost of cancer care with patients to ensure they are informed about the financial implications of treatment decisions and to support financial planning as needed.
Physical linkage and mate preference generate linkage disequilibrium for behavioral isolation in two parapatric crickets

Behavioral isolation is a potent barrier to gene flow and a source of striking diversity in the animal kingdom. However, it remains unclear if the linkage disequilibrium (LD) between sex-specific traits required for behavioral isolation results mostly from physical linkage between signal and preference loci or from directional mate preferences. Here, we test this in the field crickets Gryllus rubens and G. texensis. These closely related species diverged with gene flow and have strongly diverged songs and preference functions for the mate calling song rhythm. We map quantitative trait loci for signal and preference traits (pQTL) as well as for gene expression associated with these traits (eQTL). We find strong, positive genetic covariance between song traits and between song and preference. Our results show that this is in part explained by incomplete physical linkage: although both linked pQTL and eQTL couple male and female traits, major effect loci for different traits were never on the same chromosome. We suggest that the finely-tuned, highly divergent preference functions are likely an additional source of LD between male and female traits in this system. Furthermore, pleiotropy of gene expression presents an underappreciated mechanism to link sexually dimorphic phenotypes.
The impact of human CES1 genetic variation on enzyme activity assessed by ritalinic acid/methylphenidate ratios

The present clinical trial investigated the impact of selected SNPs in CES1 on the metabolic activity of the enzyme. For this purpose, we used methylphenidate (MPH) as a pharmacological probe and the d‐RA/d‐MPH (metabolite/parent drug) ratios as a measure of enzymatic activity. This metabolic ratio (MR) was validated against the AUC ratios (AUCd‐RA/AUCd‐MPH). CES1 SNPs from 120 volunteers were identified, and 12 SNPs fulfilling predefined inclusion criteria were analysed separately, comparing the effect of each genotype on the metabolic ratios. The SNP criteria were as follows: presence of Hardy‐Weinberg equilibrium, a minor allele frequency ≥ 0.01 and a clearly interpretable sequencing result in at least 30% of the individuals. Each participant ingested 10 mg of racemic methylphenidate, and blood samples were drawn prior to and 3 hours after drug administration. The SNP analysis confirmed the considerable impact of rs71647871 (G143E) in exon 4 on drug metabolism. In addition, three volunteers with markedly lower median MR, indicating decreased CES1 activity, harboured the same combination of three SNPs in intron 5. The median MR for these SNPs was 8.2 for the minor allele compared to 16.4 for the major alleles (P = 0.04). Hence, one of these or the combination of these SNPs could be of clinical significance considering that the median MR of the G143E group was 5.4. The precise genetic relationship of this finding is currently unknown, as is the clinical significance.
Seasonal Effects on the Corneoconjunctival Microflora in a Population of Persian Cats in Iran

&NA; This study was performed to determine the normal seasonal aerobic and an‐aerobic corneoconjunctival bacterial flora in cats. Thirty eyes of 15 clinically normal client‐owned Persian cats were evaluated. All cats lived in a similar indoor/outdoor home environment being fed the same diet for the entire year. The cats did not receive any medications and were found to be clinically healthy 1 week prior to each microbial sampling. The cats were not exposed to other cats during the study period. Microbial samples were collected at the same time of day on the first day of the second month of each of the four seasons. During sample collection, a sterile swab was rolled over the corneoconjunctival surface avoiding contact with surrounding skin or hair. Immediately after sample collection, microbiologic aerobic and anaerobic cultures were initiated. Gram‐positive bacteria were the most prevalent isolates. The most commonly isolated bacterial organisms across all seasons were Staphylococcus epidermidis (41/95; 43.2%), &bgr;‐hemolytic streptococcus (18/95; 18.9%), Staphylococcus aureus (17/95; 17.9%), and Escherichia coli (11/95; 11.5%). Twenty‐five cultures of a total of 120 (20.8%) were negative. One negative culture was collected in the summer, while 21 cultures were negative in fall and winter. Gram‐positive bacteria were the predominant micro‐organisms of the normal ocular surface of healthy cats in all seasons in this study. This result is in agreement with previous publications.
Renal Innervation in Resistant Hypertension: A Review of Pathophysiology and Renal Denervation as Potential Treatment

Background Advances in treatment and increased awareness have improved the prognosis for many patients with hypertension (HTN). Resistant hypertension (RH) refers to a subset of hypertensive individuals who fail to achieve a desired blood pressure (BP) despite concurrent use of 3 different classes antihypertensive agents, one being a diuretic, and proper lifestyle changes. The prevalence and prognosis of RH are unclear owing to its heterogeneous etiologies, risk factors, and secondary comorbidities. Previous research has provided evidence that increased renal sympathetic nerve activity (RSNA) within the renal artery contributes to RH development. Renal denervation (RDN) is a procedure that attempts to ameliorate the effects of heightened RSNA via ablation renal sympathetic fibers. BP reductions associated with RDN may be attributed to decreased norepinephrine spillover, restoration of natriuresis, increasing renal blood flow, and lowering plasma renin activity. Early clinical trials perpetuated positive results, and enthusiasm grew exponentially. However, recent clinical trials have called into question RDN's efficacy. Numerous limitations must be addressed to discern the true effectiveness of RDN as a therapeutic option for RH. Objective We aimed to review the current understanding of RH, the anatomy of renal arteries, physiology of RH on renal arteries, anatomical pathways of the sympathetic involved in RH, RDN as a treatment option, and all relevant clinical trials treating RH with RDN. Methods We piloted a MEDLINE® database search of literature extending from 1980 to 2017, with emphasis on the previous five years, combining keywords such as “resistant hypertension” and “renal denervation. ” Conclusion A plethora of information is available regarding heightened RSNA leading to RH. RDN as a possible treatment option has shown a range of results. Reconciling RDN's true efficacy requires future trials to increased sites of nerve ablation, standardized protocol, increased anatomical understanding per individual basis, stricter guidelines regarding study design, increased operator experience, and integrating the use of a multielectrode catheter.
Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model

Photodynamic Therapy (PDT) is a treatment that requires light, a photosensitizing agent, and molecular oxygen. The photosensitizer is activated by light and it interacts with the oxygen that is present in the cellular microenvironment. The molecular oxygen is transformed into singlet oxygen, which is highly reactive and responsible for the cell death. Therefore, PS is an important element for the therapy happens, including its concentration. Curcumin is a natural photosensitizer and it has demonstrated its anti-inflammatory and anti-oxidant effects that inhibit several signal transduction pathways. PDT vascular effects of curcumin at concentrations varying from 0.1 to 10 mM/cm2 and topical administration were investigated in a chick Chorioallantoic Membrane (CAM) model. The irradiation was performed at 450 nm, irradiance of 50 mW/cm2 during 10 min, delivering a total fluence of 30 J/cm2. The vascular effect was followed after the application of curcumin, with images being obtained each 30 min in the first 3 h, 12 h, and 24 h. Those images were qualitatively and quantitatively analyzed with a MatLAB®. Curcumin was expected to exhibit a vascular effect due to its angio-inhibitory effect. Using curcumin as photosensitizer, PDT induced a higher and faster vascular effect when compared to the use of this compound alone.
Comparison of Mohs Surgery and Surgical Excision in the Treatment of Localized Sebaceous Carcinoma.

BACKGROUND It remains controversial if Mohs surgery is superior to surgical excision in treating localized sebaceous carcinoma. OBJECTIVE To compare Mohs surgery and surgical excision for treating patients with localized sebaceous carcinoma. MATERIALS AND METHODS The US National Cancer Database was used to identify patients with histologically confirmed Stage 0 to 2 sebaceous carcinoma from 2004 to 2014. Clinicopathologic and socioeconomic factors were compared between treatment groups using the chi-square test. Overall survival (OS) was evaluated by log-rank test, multivariable Cox proportional hazard regression, and propensity score-matched analysis. Relative survival analyses compared with age- and sex-matched US population were performed. RESULTS Of 1,265 patients, 234 received Mohs surgery and 1,031 received surgical excision. Mohs surgery had a higher rate of negative margin (p = .004). On multivariate Cox regression analysis, Mohs surgery was associated with longer OS than surgical excision (HR: 0.703, 95% CI: 0.496-0.995, p = .047). The survival benefit of Mohs surgery persisted on relative survival analysis and propensity score-matched analysis (p = .0385), after matching the 2 groups on patient and disease characteristics. CONCLUSION Patients who received Mohs surgery had significantly longer OS when compared with those who received surgical excision. Prospective clinical trials comparing these treatment paradigms are warranted.
Age‐dependent impairment of the erythropoietin response to reduced central venous pressure in HFpEF patients

Despite growing research interest in the pathophysiology of heart failure with preserved ejection fraction (HFpEF), it remains unknown whether central hemodynamic alterations inherently present in this condition do affect blood pressure and blood volume (BV) regulation. The present study sought to determine hemodynamic and endocrine responses to prolonged orthostatic stress in HFpEF patients. Central venous pressure (CVP) assessed via the internal jugular vein (IJV) aspect ratio with ultrasonography, arterial pressure and heart rate were determined at supine rest and during 2 hours of moderate (25–30°) head‐up tilt (HUT) in 18 stable HFpEF patients (71.2 ± 7.3 years), 14 elderly (EC), and 10 young (YC) healthy controls. Parallel endocrine measurements comprised main BV‐regulating hormones: pro‐atrial natriuretic peptide, copeptin, aldosterone, and erythropoietin (EPO). At supine rest, the IJV aspect ratio was higher (>30%) in HFpEF patients compared with EC and YC, while mean arterial pressure was elevated in HFpEF patients (98.0 ± 13.1 mm Hg) and EC (95.6 ± 8.3 mm Hg) versus YC (87.3 ± 5.0 mm Hg) (P < 0.05). HUT increased heart rate (+10%) and reduced the IJV aspect ratio (−52%), with similar hemodynamic effects in all groups (P for interaction ≥ 0.322). The analysis of endocrine responses to HUT revealed a group×time interaction for circulating EPO, which was increased in YC (+10%) but remained unaltered in HFpEF patients and EC. The EPO response to a given reduction in CVP is similarly impaired in HFpEF patients and elderly controls, suggesting an age‐dependent dissociation of EPO production from hemodynamic regulation in the HFpEF condition.
JAK3 Variant, Immune Signatures, DNA Methylation, and Social Determinants Linked to Survival Racial Disparities in Head and Neck Cancer Patients

To inform novel personalized medicine approaches for race and socioeconomic disparities in head and neck cancer, we examined germline and somatic mutations, immune signatures, and epigenetic alterations linked to neighborhood determinants of health in Black and non-Latino White (NLW) patients with head and neck cancer. Cox proportional hazards revealed that Black patients with squamous cell carcinoma of head and neck (HNSCC) with PAX5 (P = 0.06) and PAX1 (P = 0.017) promoter methylation had worse survival than NLW patients, after controlling for education, zipcode, and tumor–node–metastasis stage (n = 118). We also found that promoter methylation of PAX1 and PAX5 (n = 78), was correlated with neighborhood characteristics at the zip-code level (P < 0.05). Analyses also showed differences in the frequency of TP53 mutations (n = 32) and tumor-infiltrating lymphocyte (TIL) counts (n = 24), and the presence of a specific C → A germline mutation in JAK3, chr19:17954215 (protein P132T), in Black patients with HNSCC (n = 73; P < 0.05), when compared with NLW (n = 37) patients. TIL counts are associated (P = 0.035) with long-term (>5 years), when compared with short-term survival (<2 years). We show bio-social determinants of health associated with survival in Black patients with HNSCC, which together with racial differences shown in germline mutations, somatic mutations, and TIL counts, suggests that contextual factors may significantly inform precision oncology services for diverse populations.