Miracle-dz/UniFAR-FTUs huggingface dataset:
Comprehensive survivorship care with cost and revenue analysis.

PURPOSE The 2015 Commission on Cancer standards require that cancer survivors receive an individualized survivorship care plan (SCP). To meet this new standard, St Luke's Mountain States Tumor Institute (MSTI), with support from the National Community Cancer Centers Program, implemented a successful survivorship model. PATIENTS AND METHODS At MSTI, the patient's SCP is prepared in the electronic health record by a registered health information technician. This document is reviewed during an appointment with a nurse practitioner and social worker. The provider's dictation is mailed to the primary care physician with the SCP. From August 2011 to Oct 2012, 118 patients with breast cancer were seen for survivorship appointments. Medical record audit and follow-up telephone call were completed to evaluate patient survivorship needs and satisfaction with the appointment. Patient accounts were reviewed for reimbursement. RESULTS From medical record review, the most common patient concerns were weight management (35%), fatigue (30%), sexuality (27%), anxiety (23%), caregiver stress (17%), and depression (16%). Telephone calls showed high patient satisfaction and understanding. Patients rated the following statements on a Likert scale from 1 (strongly disagree) to 5 (strongly agree): I understand my treatment summary and care plan (88% strongly agree or agree), and I feel the survivorship visit met my survivorship needs (86% strongly agree or agree). At 1 month, 80% of participants were still working on wellness goals. Patient accounts analysis showed revenue covered costs. CONCLUSION Survivorship care at MSTI meets new standards, allows for patient engagement and satisfaction, and improves care coordination. Costs are covered by reimbursement.
Characterization, expression and evolution analysis of Toll‐like receptor 1 gene in pufferfish (Tetraodon nigroviridis)

The Toll‐like receptor (TLR) family is a pathogen‐associated molecular pattern recognition receptor family that plays key roles in the activation of innate immune system. As an important member of TLRs, TLR1 is involved in the recognition of triacylated lipoproteins and mycobacterial products by binding to TLR2 to form a heterodimer. Although partial sequences of TLR1 has been predicted in Fugu and Danio by bioinformatics, the molecular cloning and characterization of this gene in fish still remains largely unclear. Here we report the full‐length sequence of TLR1 gene in the pufferfish (Tetraodon nigroviridis), providing experimental evidence to its existence in fish. The cloned Tetraodon TLR1 (TnTLR1) cDNA exhibited 2587 bp in length and contains a 122‐bp 5′ untranslated region (UTR), a 2391‐bp open reading frame and a 74‐bp 3′ UTR. Expression of TLR1 transcripts in most selected tissues were detected at lower levels; however, the transcripts in spleen was markedly increased (threefold) after stimulation with lipopolysaccharide, indicating that TLR1 in fish may be involved in the inflammatory responses to bacterial endotoxin or Gram‐negative bacteria infection. Gene organization of TnTLR1 is similar to mammalian and avian, and a high degree of conservation of chromosome synteny exists between them. The protein sequence shares 33.7–77.3% identity with known TLR1 members. Phylogenetic analysis grouped TnTLR1 with other fish TLRs on a separated branch, excluded from mammalian and avian TLR1s. TnTLR1 with 796 amino acids has a calculated molecular mass of 90.69 kDa and a theoretical pI of 8.43. Structurally, TnTLR1 has a Toll/interleukin‐1 receptor (TIR) domain, a transmembrane domain and leucine‐rich repeat domain which are the hallmarks of TLR family. Phylogenetic analysis provided evidence that the TnTLR1 was very close to mammalian TLR1, TLR6 and TLR10 and seemed to be their common ancestor. This report of the full‐length TLR1 gene in Tetraodon will provide us further insights into the study of both function and evolution of fish TLRs as a whole.
The 1A protein of respiratory syncytial virus is an integral membrane protein present as multiple, structurally distinct species

The respiratory syncytial virus (RSV) 1A protein was previously identified as a 7.5-kilodalton (kDa) nonglycosylated species that, on the basis of its predicted sequence determined from the sequence of its mRNA, contains a hydrophobic central domain that was suggestive of membrane interaction. Here, four major, structurally distinct intracellular species of the 1A protein were identified in cells infected by RSV or by a recombinant vaccinia virus expressing the 1A gene. The four species of 1A were: (i) the previously described, nonglycosylated 7.5-kDa species that appeared to be the full-length, unmodified 1A protein; (ii) a nonglycosylated 4.8-kDa species that was carboxy-coterminal with the 7.5-kDa species and might be generated by translational initiation at the second AUG in the sequence; (iii) a 13- to 15-kDa species that contained one or two N-linked carbohydrate side chains of the high-mannose type; and (iv) a 21- to 30-kDa glycosylated species that appeared to be generated from the 13- to 15-kDa species by further modification of the N-linked carbohydrate. All four forms of the 1A protein were synthesized and processed on intracellular membranes, and several lines of biochemical evidence showed that all four species were integral membrane proteins. Thus, the 1A protein is a third RSV integral membrane protein and is present as such in both glycosylated and nonglycosylated forms. With the use of antiserum raised against a synthetic peptide representing the C terminus of the 1A protein, indirect immunofluorescence showed that the 1A protein was expressed at the cell surface. Antibody-antigen complexes formed at the surface of intact infected cells were immunoprecipitated, showing that the 7.5-kDa, 13- to 15-kDa, and 21- to 30-kDa, but not the 4.8-kDa, species, were accessible to extracellular antibodies. Thus, the 1A protein is a candidate to be a viral surface antigen. The small size, gene map location integral membrane association, and cell surface expression of the 1A protein strongly suggested that it is a counterpart to the SH protein that has been described for simian virus type 5. We suggest that, in the future, the RSV 1A protein be given the same designation, namely, SH.
High incidence of EMMPRIN expression in human tumors

Extracellular matrix metalloproteinase inducer expressed by tumor cells stimulates peritumoral fibroblasts to produce matrix metalloproteinases, thus contributing to tumor invasion and metastasis. To assess its suitability as potential therapeutic target, the overall incidence of EMMPRIN expression in normal and neoplastic tissues was analyzed. EMMPRIN expression was detected immunohistochemically using monoclonal antibodies MEM‐M6/1 and HIM6 and tissue microarrays with 2,348 and 608 tissue samples from 129 distinct tumor types and 76 different normal tissues, respectively. Expression and glycosylation state of EMMPRIN in human breast cancer cells were analyzed by Western blot analysis with monoclonal antibodies recognizing distinct carbohydrate structures and biochemical methods. EMMPRIN expression was found in 112 of 129 tumor entities analyzed with malignant tumors being EMMPRIN positive more frequently than benign tumors. A remarkable heterogeneity in EMMPRIN expression between tumor entities was observed. Among others, squamous‐cell carcinomas (60–100%), pancreatic (87%), chromophobic kidney (83%), hepatocellular (83%) or medullary breast (83%) adenocarcinomas as well as glioblastoma multiforme (79%) presented with a particular high incidence of EMMPRIN expression. There were a limited number of EMMPRIN‐positive normal cell types including proliferatively active and differentiating epithelial cells, germ cells, myocardial cells in the left heart ventricle or vascular endothelial cells of the brain. We could further demonstrate that breast cancer cells expressed EMMPRIN isoforms differing in the presence or absence of LewisX glycan structures. Our results may assist in defining the suitability of EMMPRIN as therapeutic target and predicting negative side effects. © 2006 Wiley‐Liss, Inc.
Precise limitation of concerted evolution to ORFs in mosquito Hsp82 genes

Two Hsp82 genes were isolated from the malaria vector Anopheles albimanus in a single lambda phage clone. The two genes are in a head‐to‐head arrangement separated by approx. 0.9 kbp. Northern hybridizations and 5' RACE demonstrate that both genes are transcribed, have moderate levels of constitutive transcription, and are also heat‐inducible with maximum transcript accumulation occurring after 40°C heat shocks. Both genes have typical heat‐shock promoters and conserved intron boundaries in the untranslated leaders. The open reading frames are 99.6% identical differing in only nine silent nucleotide positions in the 2166 bp ORFs. However, precisely outside the ORFs, the flanking DNA of the two genes shows no evidence of common derivation. The high degree of identity between the two ORFs appears to be a result of gene conversion occurring by a process similar to that previously suspected in the A. albimanus Hsp70 genes and several D. melanogaster genes arranged as palindromes. This process probably involves a stem‐loop intermediate and is restricted in extent by flanking sequence divergence. These Hsp82 genes clearly demonstrate the extreme precision with which gene conversion can lead to proteincoding‐region homogeneity yet allow flanking DNA divergence.
Rapid screening for early detection of mother-to-child transmission of human immunodeficiency virus type 1

The testing of dried blood spots (DBSs) for the presence of human immunodeficiency type 1 (HIV-1) proviral DNA by PCR was first described in 1991. The technology has proven to be particularly valuable for resolving the infection status in HIV-1-indeterminate infants born to HIV-1-seropositive mothers. To broaden the applicability of DBS PCR, we adapted it to a standardized, commercially available microwell plate amplification and detection kit, Amplicor HIV-1, produced by Roche Diagnostic Systems. The microwell assay is rapid and easy to perform and uses equipment that is readily available in routine diagnostic laboratories. The high level of performance of the assay was demonstrated in 1,168 duplicate tests performed on 584 DBSs from 178 uninfected and 100 HIV-1-infected individuals, including 56 children with perinatally acquired HIV-1. Of 12 infants who were followed prospectively from birth, 3 (25%) were infected in utero (PCR positive at birth) and 9 (75%) were infected intrapartum (PCR negative, culture negative at birth). Overall, HIV-1 DNA was identified in 3 of 11 (27.3%) DBSs collected from infected infants during the first 4 days of life, 8 of 9 (88.9%) DBSs collected between 10 and 15 days postpartum, and 166 of 167 (99.4%) DBSs collected after 15 days of age. All 320 DBSs from uninfected children were PCR DNA negative. These findings indicate that use of the Roche microwell DBS PCR assay provides a powerful new approach for large-scale perinatal screening programs and population-based studies of vertical transmission.
IL-1beta and IFN-gamma induce the expression of diverse chemokines and IL-15 in human and rat pancreatic islet cells, and in islets from pre-diabetic NOD mice.

AIMS/HYPOTHESIS Cytokines and chemokines are important mediators of immune responses due to their ability to recruit and activate leukocytes. Using microarray analysis we observed that rat beta cells exposed to IL-1beta and IFN-gamma have increased mRNA levels of chemokines and IL-15. The aim of this study was to characterize the expression of IP-10, MIP-3alpha, fractalkine and IL-15 in rat beta cells, human pancreatic islets, and in islets isolated from NOD mice, both during the pre-diabetic period and following islet transplantation. METHODS FACS-purified rat beta cells and human islets were cultured with IL-1beta, IFN-gamma and/or TNF-alpha. Islets were isolated from NOD or BALB/c mice at different ages. For syngeneic islet transplantation, 2- or 3-week-old NOD islets were grafted under the kidney capsule of spontaneously diabetic NOD recipients. Chemokine and IL-15 mRNA expression and protein release were evaluated, respectively, by RT-PCR and ELISA. RESULTS Human islets and rat beta cells express IP-10, MIP-3alpha, fractalkine and IL-15 mRNAs upon exposure to cytokines. The expression of IL-15, IP-10 and fractalkine is regulated by IFN-gamma, while the expression of MIP-3alpha is IL-1beta-dependent. Moreover, cytokines induced IL-15, IP-10, Mig, I-TAC and MIP-3alpha protein accumulation in culture medium from human islets. In vivo, there was an age-related increase in IL-15, IP-10 and MIP-3alpha expression in islets isolated from NOD mice. Following syngeneic islet transplantation, increased expression of IL-1beta, IFN-gamma, fractalkine, IP-10, MCP-1 and MIP-3alpha mRNAs were observed in the grafts. CONCLUSION/INTERPRETATION Cytokine-exposed islets or beta cells express chemokines and IL-15. This could contribute to the recruitment and activation of mononuclear cells and development of insulitis in early Type 1 diabetes and during graft destruction.
Loss of heterozygosity on the long arm of chromosome 22 in pheochromocytoma

To identify the putative common deleted region on the long arm of chromosome 22 in pheochromocytoma, restriction fragment length polymorphism analysis was performed in 17 pheochromocytomas. All cases were heterozygous for at least one of the eight marker loci on 22q. Loss of heterozygosity (LOH) was observed in nine pheochromocytomas, of which eight were hereditary and one nonhereditary. Three pheochromocytomas had interstitial deletions that enabled us to localize the commonly deleted region as distal to D22S10 and proximal to D22S22. Hereditary pheochromocytoma frequently occurs in association with medullary thyroid carcinoma (MTC). Therefore, we also studied allelic loss on 22q in 23 hereditary MTCs. Only one of the MTCs showed LOH on 22q. Recent studies have mapped tumor suppressor loci associated with meningioma and neurofibromatosis type 2 (NF2) to 22q. The commonly deleted region in pheochromocytoma found by us encompasses the regions to which tumor suppressor genes associated with NF2 and meningioma have been mapped. The exact role of the pheochromocytoma tumor suppressor gene on 22q and its relationship to the suppressor genes involved in NF2 and meningioma remain unknown. © 1992 Wiley‐Liss, Inc.
Visual Function in Young IDDM Patients Over 8 Years of Age: A 4-year longitudinal study

OBJECTIVE To carry out a longitudinal study of visual functions in young patients over the age of 8 years with IDDM and to assess the impact of metabolic control on the presence of diabetic retinopathy. RESEARCH DESIGN AND METHODS There were 37 young IDDM patients from the Paediatric and Adolescent Clinic at the University Hospital of Wales studied annually for 4 years, with a control group of 24 healthy subjects observed over a 2-year period. Assessment of visual functions included visual acuity, color vision, and contrast sensitivity. Ophthalmoscopy and retinal photography were used to determine the presence or absence of diabetic retinopathy. In addition, pubertal status and metabolic control (glycosylated hemoglobin) were determined at each visit. RESULTS Patients with IDDM demonstrate abnormal color vision and contrast sensitivity compared with the control group (P < 0.05), but visual acuity was unaffected. Visual functions were not significantly different between those IDDM patients with and without retinopathy. After 4 years, diabetic retinopathy was present in 43% of the group and was related to diabetes duration and metabolic control (P < 0.05). CONCLUSIONS Visual function testing could not distinguish between those IDDM patients with and without retinopathy, but the color vision and contrast sensitivity in those with IDDM were significantly impaired compared with the control group. The presence of retinopathy was related to the duration of diabetes and metabolic control. It is important to ensure that good glycemic control and regular attendance for retinopathy screening is encouraged in the adolescent patients.
An Improved Mass Determination for M31 from Its Satellite Galaxies

The High-Resolution Echelle Spectrometer on the Keck I telescope has been used to measure the first radial velocities for stars belonging to the Andromeda I and III dwarf spheroidal galaxies. Our radial velocity for And III matches that reported by Blitz & Robishaw for an adjacent H I gas complex, supporting the association of this galaxy with a high-velocity cloud. New and previously published radial velocities for a sample of confirmed or suspected M31 satellites are combined with a homogeneous set of distance estimates to calculate the total mass of M31. Assuming the satellite orbits are isotropic, we find a median mass of MA ≃ (7.9 ± 0.5) × 1011 M☉ from eight candidate satellites having deprojected distances from M31 in excess of RA ≃ 100 kpc. If the orbits are radial, the inferred mass increases to MA ≃ (21.5 ± 3.8) × 1011 M☉; for circular orbits, the enclosed mass is MA ≃ (3.7 ± 0.4) × 1011 M☉. These masses are somewhat lower than those found for luminous spiral galaxies based on the ensemble dynamics of the their satellites, although the estimates are nevertheless consistent given the large uncertainties.
Making innovative use of academic knowledge to enhance corporate technology innovation impact

This paper reviews 100 papers on the topic of IndustryUniversity (I/U) collaborations to identify the drivers and main-barriers to such collaborations. It reveals that the greatest challenges from the companies' point-of-view reside in steering the collaborative project towards business objectives, and internalising the results for corporate technology innovation impact. Our review also reveals that there is only limited advice in the literature on how to handle these challenges. New knowledge is developed through deduction from theory and induction from qualitative in-depth studies with ten leaders of innovation in high-tech industries. The most relevant case is described in this paper illustrating how Bang and Olufsen (B&O) spun off their core technology into a separate company so as to radically improve their steering and internalisation of academic knowledge in this area. Managerial conclusions are drawn from the theoretical framework and the case to suggest how to explore and exploit academic knowledge for global technology innovation impact.
Clostridium perfringens type A enterotoxin induces tissue damage and fluid accumulation in rabbit ileum.

Rabbit ileal loops were treated with purified Clostridium perfringens enterotoxin (CPE) to compare the onset of toxin-induced tissue damage with the onset of fluid transport changes (i.e. diarrhoea). Mild changes in fluid transport were detectable after 15 minutes of toxin treatment and then increased progressively with time. Histopathologic studies on toxin-treated ileal loops and measurement of toxin-treated loop fluid protein contents (an indirect marker for tissue damage) both indicated that detectable CPE-induced tissue damage also occurred within 15 minutes of toxin treatment and then progressively increased for at least the first 30 minutes of CPE treatment. Luminal fluid from CPE-treated loops contained elevated Ca2+ levels compared to control luminal fluid, but these elevated Ca2+ levels were not required to initiate in vivo CPE pathophysiologic effects. Since the onsets and severity of tissue damage and fluid transport changes coincided for at least the first 30 minutes of CPE treatment in our study, these results are consistent with CPE-induced tissue damage having a role in the initiation and extent of diarrhoea occurring during C. perfringens type A food poisoning.
Human-Dog Interactions in a Guide-Dog Training Program

We analyzed dyadic interactions between 12 neutered dogs (6 females and 6 males) and 44 humans (20 women, 14 men, and 10 girls) who were unfamiliar with each other. We also examined the effect of sex differences in dogs and humans as well as age differences in humans on human-dog interactions in a guide-dog turning program. Female dogs more actively regulated their distance from humans than male dogs. Dogs made contact with women more frequently than with men, and men nude contact with dogs more frequently than women. Girls initiated interactions with dogs more frequently than women; girls formed reciprocal interactions with dogs less frequently than women.
THE EFFECT OF ADRENERGIC β‐RECEPTOR BLOCKADE ON CORONARY CIRCULATION IN MAN DURING WORK

The effect of an adrenergic β‐receptor blocker (sotalol) on coronary circulation and heart metabolism has been studied in six healthy subjects at rest and during work. Sotalol did not produce any uniform effects at rest. During work sotalol reduced the increase of cardiac output, oxygen consumption and coronary blood flow as well as the lowering of the total peripheral resistance which is produced by muscle activity. Despite the fact that sotalol reduced the work of the heart musculature and its oxygen consumption, oxygen extraction from coronary blood was increased. The pyruvate/lactate quotient in coronary venous blood was decreased during work by sotalol. The mechanism for reduction of metabolic vasodilatation by sotalol is discussed. It seems probable that after β‐receptor blockade the contracting effect of adrenergic α‐receptor stimulation gains an increased significance.
A checklist of the Cymothoidae (Crustacea, Isopoda) recorded from Indian fishes

A checklist of the parasitic Cymothoidae of Indian fishes was compiled from parasitological records published between 1783 and 2011. The checklist is arranged alphabetically, providing valid names, synonyms and authorities of the parasite species, as well as valid names and synonyms of the host fish, its capture sites, author(s) and date of published records. The host list consists of all parasites species listed under the host species. A total of 47 nominal species corresponding to 36 valid species are listed from 74 host species belonging to 34 families. Several parasites not identified to species level and parasite species without the host data or where the parasite was found not associated with a fish are also included in this checklist.
Coxibs and cardiovascular disease.

The coxibs are a subclass of nonsteroidal antiinflammatory drugs (NSAIDs) designed to inhibit selectively cyclooxygenase-2 (COX-2). 1 Their development was based on the hypothesis that COX-2 was the source of prostaglandins E2 and I2, which mediate inflammation, and that cyclooxygenase-1 (COX-1) was the source of the same prostaglandins in gastric epithelium, where they afford cytoprotection. Three coxibs — celecoxib, rofecoxib, and valdecoxib — have been approved for use by the Food and Drug Administration (FDA); a fourth, etoricoxib, has been approved by the European regulatory authority, and it and a fifth, lumiracoxib, are currently under consideration for FDA .
Communication Patterns in Medical Encounters for the Treatment of Child Psychosocial Problems: Does Pediatrician–Parent Concordance Matter?

This study examined how pediatrician–parent social status concordance related to communication patterns in medical encounters during which children received treatment for psychosocial problems indicating attention deficit disorder or attention deficit hyperactivity disorder. Using data from 28 pediatric medical encounters occurring in a large southeastern metropolitan city during 2003, we focused on concordance according to race, gender, and education, and its relation to laughter, concern, self-disclosure, question asking, and information-giving utterances, and patient-centeredness. Results indicated that race-concordant pediatricians and parents frequently laughed, whereas parents asked many biomedical questions in gender-concordant encounters. Education-concordant pediatricians and parents expressed concern repeatedly, exchanged biomedical information freely, and shared communication control. Pediatricians also self-disclosed when interacting with college-educated parents.