Miracle-dz/UniFAR-FTUs huggingface dataset:
Rigor and reproducibility via laboratory studies of eating behavior: A focused update and conceptual review.

OBJECTIVE The eating behavior of individuals with eating disorders has been examined in laboratory settings over the last 30 years. In this focused review, we build on prior research and highlight several feeding laboratory paradigms that have successfully demonstrated quantifiable and observable behavioral disturbances, and thereby add rigor and reproducibility to the examination of disturbances of eating behavior. This review describes the measures commonly obtained via these laboratory techniques. Supporting Information Appendices with detailed information about implementation are provided to allow for the reproducible execution of these techniques across labs. METHODS/RESULTS Literature documenting the existence of objective abnormalities in eating behavior among individuals with eating disorders or in comparison to healthy controls (n > 40) is briefly summarized. These protocols, conducted across at least 17 independent labs, are sensitive and reproducible, can be used to assess subjective and physiological parameters associated with eating, and elucidate the impact of treatment. Laboratory studies from patients with eating disorders compared with healthy controls reproducibly demonstrate both that patients with Anorexia Nervosa ingest fewer calories and that individuals with Bulimia Nervosa and Binge-Eating Disorder ingest more calories when asked to binge-eat. DISCUSSION Feeding laboratory studies have the potential for quantifying the characteristic behavioral psychopathology of patients with eating disorders, and may provide a useful tool to explore the potential utility of new treatments for individuals with Anorexia Nervosa, Bulimia Nervosa, and Binge-Eating Disorder.
Choosing Wisely clinical decision support adherence and associated inpatient outcomes.

OBJECTIVES To determine whether utilization of clinical decision support (CDS) is correlated with improved patient clinical and financial outcomes. STUDY DESIGN Observational study of 26,424 patient encounters. In the treatment group, the provider adhered to all CDS recommendations. In the control group, the provider did not adhere to CDS recommendations. METHODS An observational study of provider adherence to a CDS system was conducted using inpatient encounters spanning 3 years. Data comprised alert status (adherence), provider type (resident, attending), patient demographics, clinical outcomes, Medicare status, and diagnosis information. We assessed the associations between alert adherence and 4 outcome measures: encounter length of stay, odds of 30-day readmission, odds of complications of care, and total direct costs. The associations between alert adherence and the outcome measures were estimated using 4 generalized linear models that adjusted for potential confounders, such as illness severity and case complexity. RESULTS The total encounter cost increased 7.3% (95% CI, 3.5%-11%) for nonadherent encounters versus adherent encounters. We found a 6.2% (95% CI, 3.0%-9.4%) increase in length of stay for nonadherent versus adherent encounters. The odds ratio for readmission within 30 days increased by 1.14 (95% CI, 0.998-1.31) for nonadherent versus adherent encounters. The odds ratio for complications increased by 1.29 (95% CI, 1.04-1.61) for nonadherent versus adherent encounters. CONCLUSIONS Consistent improvements in measured outcomes were seen in the treatment group versus the control group. We recommend that provider organizations consider the introduction of real-time CDS to support adherence to evidence-based guidelines, but because we cannot determine the cause of the associations between CDS interventions and improved clinical and financial outcomes, further study is required.
In vitro chromosomal radiosensitivity in patients with common variable immunodeficiency

Common variable immunodeficiency (CVID) is one of the predominant antibody deficiency disorders, some evidence of which indicates that chromosome instability is present in these patients. An increased risk of cancer in patients with CVID has been documented. This study was undertaken to highlight radiation sensitivity in CVID patients and to clarify the genetic basis of this defect in these cases. Stimulated lymphocytes of the studied subjects were exposed to low-dose gamma-rays in the G2 phase or the G0 phase of the cell cycle and chromosomal aberrations were scored. Lymphocytes of healthy individuals, ataxia telangiectasia (AT) cases and a group of acute lymphoblastic leukemia (ALL) patients were investigated in the same way as controls. By two methods of analysis (one-way ANOVA and unpaired t-test), the CVID cases were significantly more radiosensitive than healthy controls based on the results of the G2 and the G0 assays. First-degree relatives of CVID patients were radiosensitive by the micronucleus assay which showed a significant difference as compared with normal controls (p = 0.001). In conclusion, this study may support that chromosomal radiosensitivity in CVID patients is a marker of genetic predisposition to the disease. The results might be a clue to describe the increased risk of cancer in CVID patients.
An allogenic therapeutic strategy for canine spinal cord injury using mesenchymal stem cells

This study was conducted to characterize canine bone marrow‐derived mesenchymal stem cells (BMSCs); in vivo tracking in mice, and therapeutic evaluation in canine clinical paraplegia cases. Canine BMSCs were isolated, cultured, and characterized in vitro as per International Society for Cellular Therapy criteria, and successfully differentiated to chondrogenic, osteogenic, and adipogenic lineages. To demonstrate the homing property, the pGL4.51 vector that contained luciferase reporter gene was used to transfect BMSCs. Successfully transfected cells were injected around the skin wound in mice and in vivo imaging was done at 6, 12 and 24 hr post MSCs delivery. In vivo imaging revealed that transfected BMSCs migrated and concentrated predominantly toward the center of the wound. BMSCs were further evaluated for allogenic therapeutic potential in 44 clinical cases of spinal cord injuries (SCI) and compared with conventional therapy (control). Therapeutic potential as evaluated by different body reflexes and recovery score depicted significantly better results in stem cell‐treated group compared to control group. In conclusion, allogenic canine BMSCs can serve as potent therapeutic candidate in cell‐based therapies, especially for diseases like SCI, where the conventional medication is not so promising.
MicroRNA-221 silencing attenuates the degenerated phenotype of intervertebral disc cells

The aim of this study was to investigate the role of an antichondrogenic factor, MIR221 (miR-221), in intervertebral disc degeneration (IDD), and provide basic information for the development of a therapeutic strategy for the disc repair based on specific nucleic acid based drugs, such as miR-221 silencing. We established a relatively quick protocol to minimize artifacts from extended in vitro culture, without selecting the different types of cells from intervertebral disc (IVD) or completely disrupting extracellular matrix (ECM), but by using the whole cell population with a part of resident ECM. During the de-differentiation process miR-221 expression significantly increased. We demonstrated the effectiveness of miR-221 silencing in driving the cells towards chondrogenic lineage. AntagomiR-221 treated cells showed in fact a significant increase of expression of typical chondrogenic markers including COL2A1, ACAN and SOX9, whose loss is associated with IDD. Moreover, antagomiR-221 treatment restored FOXO3 expression and increased TRPS1 expression levels attenuating the severity grade of degeneration, and demonstrating in a context of tissue degeneration and inflammation not investigated before, that FOXO3 is target of miR-221. Data of present study are promising in the definition of new molecules useful as potential intradiscal injectable biological agents.
Comparative analyses of the complete mitochondrial genomes of Cyathostomum pateratum and Cyathostomum catinatum provide new molecular data for the evolution of Cyathostominae nematodes

Abstract The parasite Cyathostomum pateratum, which occurs in the large intestine of equines, is a common species of the subfamily Cyathostominae. Cyathostominae nematodes are a complex nematode group for which only limited genetic information has been reported. To re-examine the phylogenetic relationships among Cyathostominae nematodes, we sequenced the complete mitochondrial (mt) genome of Cy. pateratum and compared it with the mt genome of the congeneric species Cyathostomum catinatum. The complete mtDNA sequence of Cy. pateratum was 13,822 bp in length, 16 bp shorter than that of Cy. catinatum. The mtDNA sequences of both species contained 12 protein-coding genes, two rRNA genes and 22 tRNA genes, and all 36 genes were transcribed in the same direction and in the same strand. Pairwise comparisons of the 12 predicted amino acid sequences between Cy. catinatum and Cy. pateratum revealed differences of 0.4–3.1%; the least conserved sequence was that of cytochrome c oxidase subunit 3 (cox3). Phylogenetic analysis of the concatenated amino acid sequences using Bayesian inference and maximum likelihood methods showed that Cy. catinatum and Cy. pateratum clustered together with very high nodal support, and Cylicostephanus goldi was closer to the Cyathostomum nematodes than to other Cyathostominae nematodes. The mtDNA sequence of Cy. pateratum is reported here for the first time. The study will shed some light on the genetic evolution among parasitic nematodes in Cyathostomum.
Long noncoding RNA SNHG16 contributes to the development of bladder cancer via regulating miR‐98/STAT3/Wnt/β‐catenin pathway axis

This study aimed to investigate the role and the possible mechanism of the long noncoding small nucleolar RNA host gene 16 (SNHG16) in bladder cancer development. The expression of SNHG16 in the tumor tissues and plasma of patients with bladder cancer as well as bladder cancer cell lines was detected. T24 cells were then transfected with sh‐SNHG16 to further investigate the effects of suppression of SNHG16 on T24 cell proliferation, apoptosis, migration, and invasion. In addition, the regulatory relationships between SNHG16 and miR‐98 as well as the target of miR‐98 were explored. Besides, the association between SNHG16 and the Wnt/β‐catenin pathway was further elucidated. The SNHG16 expression was upregulated in the tumor tissues and plasma of patients with bladder cancer, as well as bladder cancer cells. Suppression of SNHG16 inhibited T24 cell proliferation, promoted apoptosis, and suppressed migration and invasion in vitro. In addition, SNHG16 negatively regulated miR‐98 expression and regulated the malignant behaviors of T24 cells through sponging miR‐98. Moreover, signal transducer and activator of transcription 3 (STAT3) was identified as a functional target of miR‐98, and miR‐98 regulated the malignant behaviors of bladder cancer cells by targeting STAT3. Besides, suppression of SNHG16 inhibited the activation of the Wnt/β‐catenin pathway, which was further regulated by miR‐98 and STAT3, indicating that the effects of SNHG16/miR‐98/STAT3 on T24 cells were achieved through the Wnt/β‐catenin pathway. Our findings reveal that long noncoding RNAs SNHG16 is upregulated in bladder cancer and contributes to the development of bladder cancer possibly via regulating the miR‐98/STAT3/Wnt/β‐catenin pathway axis. The SNHG16/miR‐98/STAT3/Wnt/β‐catenin pathway axis may provide a new strategy for bladder cancer treatment.
A qualitative investigation of the role of paediatric rehabilitation professionals in rural South Africa: Rehabilitation professionals’ perspectives

Purpose To investigate the role that rehabilitation professionals play in the rehabilitation of children with disabilities in the rural and under-resourced community of Giyani in South Africa. Method A qualitative, exploratory and descriptive approach was used. Semi-structured face-to-face interviews were used to collect data from a convenient sample of eight rehabilitation professionals. Data were transcribed verbatim by two trained students and verified by the main researcher. An inductive approach to qualitative data analysis was used. In vivo and open coding were used to generate codes. Results Analysis of data resulted in 21 codes, 9 subcategories, 5 categories and 1 theme. The role of rehabilitation professionals was described in terms of the five categories which are to examine newborn babies and children at risk, support caregivers of children with disabilities, impart skills training for caregivers of children with disabilities, rehabilitate children with disabilities and conduct follow-ups in communities where the children with disabilities reside. Conclusion The role that rehabilitation professionals play in the rural and under-resourced community of Giyani in South Africa is similar to the role played in high-income countries. The role that rehabilitation professionals play is not only focused on the child but also on the family.
Stretchable Optical Sensing Patch System Integrated Heart Rate, Pulse Oxygen Saturation, and Sweat pH Detection

<italic>Objective:</italic> Continuous measurement of key physiological parameters, such as heart rate (HR), pulse oxygen saturation (SpO<sub>2</sub>), and sweat pH value, has very broad applications in healthcare, disease surveillance, and fitness and sports training. In this study, a stretchable optical sensing patch system was developed for real-time continuous noninvasive monitoring of the HR, SpO<sub>2,</sub> and sweat pH, and the sensing data were transmitted to a smartphone through Bluetooth. <italic>Methods</italic>: The sensor patch system adopted serpentine stretchable interconnects between the optical sensor and microcontroller chip with wireless function on a flexible substrate. The pH sensing function was implemented by coating a pH sensitive organically modified silicate film on the surface of a commercial blood oxygen sensor, achieving simultaneous measurement of HR, SpO<sub>2,</sub> and sweat pH with a single sensor. <italic>Results</italic>: Real-time on-body assessment was carried out to evaluate the sensor patch system, showing its excellent repeatability and applicability. The sensor patch system could withstand up to 35% extension and exhibited a pH sensitivity of 4.42 mV/pH from 4.0 to 8.0, while the accuracy of HR from 25 to 250 b/min and SpO<sub>2</sub> from 70% to 100% sensing were ±1 b/min and ±2%, respectively. <italic>Conclusion</italic>: The triple sensing functions was achieved through a single optical sensor on a flexible substrate while holding excellent contact with the body. <italic>Significance</italic>: The sensor patch system can be used for fitness guidance, skin disease detection, and wound monitoring and management by replacing related sensitive films.
Combined transcranial direct current stimulation and breathing-controlled electrical stimulation for management of neuropathic pain after spinal cord injury.

OBJECTIVE To determine whether transcranial direct current stimulation augments the analgesic effect of breathing-controlled electrical stimulation in patients with spinal cord injury who have chronic neuropathic pain. DESIGN Sham-controlled, single-blinded, single-centre, cross-over study of 12 participants with incomplete spinal cord injury. The treatment protocol included a 20-min transcranial direct current stimulation (sham or active), followed by a 20-min breathing-controlled electrical stimulation to the median nerve on the dominant side. The treatment session with sham or control transcranial direct current stimulation was given on different days in a randomized order. Visual analogue scale was used to assess neuropathic pain at baseline, 10 min after transcranial direct current stimulation, and 10 min after breathing-controlled electrical stimulation. RESULTS Participants were blinded to the status of transcranial direct current stimulation. Out of the 12 participants, 10 completed sessions of both sham and active transcranial direct current stimulation, while the other 2 completed only active transcranial direct current stimulation and breathing-controlled electrical stimulation treatment. Out of the 12 participants, 7 showed analgesic effects after active transcranial direct current stimulation, while sham transcranial direct current stimulation produced some analgesic effects in 4 out of 10 participants. At the group level, there was no difference between active and sham transcranial direct current stimulation treatment. All except one participant responded positively to breathing-controlled electrical stimulation in all sessions. Visual analogue scale score for pain decreased significantly after breathing-controlled electrical stimulation combined with either active transcranial direct current stimulation or sham transcranial direct current stimulation treatment. CONCLUSION The immediate analgesic effect of breathing-controlled electrical stimulation was confirmed. However, this effect was not augmented after one session of transcranial direct current stimulation treatment.
Are Parents Patient? The Influence of Parenting Role Salience and Parental Status on Impatience

In the classic intertemporal discounting task (Thaler, 1981), individuals make tradeoff decisions between smaller-sooner and larger-later monetary rewards. We explored how parental role salience and parental status influences individual’s choice between smaller-sooner and larger-later choices. Parental role salience is manipulated among both parents and non-parents in this research. Our results show a significant interaction between parental status and manipulated parental role salience. Specifically, we found that parents are more impatient than non-parents. Additionally, non-parents become more impatient after parental role salience manipulation, similar to parents. Theoretical implications of our findings are discussed.
Underestimation of treatment effects in sequentially monitored clinical trials that did not stop early for benefit

In recent years, there has been a prominent discussion in the literature about the potential for overestimation of the treatment effect when a clinical trial stops at an interim analysis due to the experimental treatment showing a benefit over the control. However, there has been much less attention paid to the converse issue, namely, that sequentially monitored clinical trials which did not stop early for benefit tend to underestimate the treatment effect. In meta-analyses of many studies, these two sources of bias will tend to balance each other to produce an unbiased estimate of the treatment effect. However, for the interpretation of a single study in isolation, underestimation due to interim analysis may be an important consideration. In this paper, we discuss the nature of this underestimation, including theoretical and simulation results demonstrating that it can be substantial in some contexts. Furthermore, we show how a conditional approach to estimation, in which we condition on the study reaching its final analysis, may be used to validly inflate the observed treatment difference from a sequentially monitored clinical trial. Expressions for the conditional bias and information are derived, and these are used in supplied R code that computes the bias-adjusted estimate and an associated confidence interval. As well as simulation results demonstrating the validity of the methods, we present a data analysis example from a pivotal clinical trial in cardiovascular disease. The methods will be most useful when an unbiased treatment effect estimate is critical, such as in cost-effectiveness analysis or risk prediction.
A New Combined CNN-RNN Model for Sector Stock Price Analysis

The combination of Convolutional Neural Networks (CNNs) and Recurrent Neural Networks (RNNs) have played important roles in deep learning in recent years to improve the prediction performance, especially in the context of temporal data analysis. Previous research has shown that certain time series could have common time-dependent characteristics. Therefore, in order to make good prediction, it is necessary to take into account the correlation between different temporal data in modeling. However, general RNN models have serious limitation to achieve this goal. In this paper, a new architecture, Deep and Wide Neural Networks (DWNN), is proposed, where CNN's convolution layer is added to the RNN's hidden state transfer process. CNN is combined with RNN to extract the correlation characteristics of different RNN models while RNNs running along the time steps. This new architecture not only has the depth of RNN in the time dimension, but also has the width of the number of temporal data. The intuition behind the DWNN model, as well as different kinds of DWNN model structures are discussed in this paper. We use stock data from the sandstorm sector of Shanghai Stock Exchange for our experiment. As shown in the result, our proposed DWNN model can reduce the prediction mean squared error by 30% compared with the general RNN model.
Association between the symptoms of benign prostatic hyperplasia and social disparities: Does social capital promote prostate health?

This cross‐sectional study investigated the relationships between socioeconomic factors and social capital and benign prostatic hyperplasia symptoms. The participants were 100,000 adult men who participated in the Korea Community Health Survey. The surveyors used the International Prostate Symptom Score. As regards occupation, the prevalence of benign prostatic hyperplasia was higher in men with blue‐collar occupations or those who were unemployed than in those with white‐collar jobs. In terms of marital status, the prevalence of benign prostatic hyperplasia was 1.319 times higher among divorced men than married men. As regards social capital, the prevalence of benign prostatic hyperplasia in men with positive attitudes towards one's community scores that reflected good, poor and very poor community scores was 1.228, 1.246 and 1.447 times higher than that of men who had very good scores respectively. The groups with good, poor, and very poor community participation scores had 1.115, 1.202 and 1.364 times higher prevalence of benign prostatic hyperplasia than the group with very good scores. Social disparities and social capital of a community were associated with the prevalence of benign prostatic hyperplasia. Thus, the use of social capital in the community setting will be effective in the management of the condition.
Age-related Changes in Training Stimuli and Performance in Masters Swimmers

Abstract Age-related decreases in performance are thought to be driven by decreases in exercise training stimuli. We determined the influence of changes in training stimuli with advancing age on swimming performance using cross-sectional and longitudinal data analyses. Totals of 692 and 98 competitive swimmers belonging to the US Masters Swimming Association were analyzed in cross-sectional and longitudinal analyses. Swimming times increased with advancing age, and age was the strongest predictor of swimming performance, followed by training volume, in both the cross-sectional and longitudinal analyses. In the cross-sectional data analyses, an increase in training volume by 10 km/month, was associated with improved performance by 0.69 s regardless of age. In the longitudinal analyses, training volume was not a significant predictor for younger swimmers. In middle-aged swimmers, however, increases in training volume resulted in faster swimming times, and its effect was more pronounced in older swimmers. We concluded that there was a graded positive relationship between yearly increases in training volume and improved swimming performance, and that such effects were greater with advancing age.