Miracle-dz/UniFAR-FTUs huggingface dataset:
The Relationship Between Active and Neural Measures in Patients With Nonspecific Low Back Pain

Study Design. Cross-sectional study of 50 individuals with nonspecific low back pain (LBP). Objective. To evaluate the relationship between active and neural measurements in individuals with nonspecific LBP. Summary of Background Data. The Panjabi model for spinal stability consists of 3 interacting subsystems. Specific musculoskeletal measurements can be identified within the active and neural systems. The relationships among measures within this model have not been identified. Methods. A total of 50 individuals with nonspecific LBP were evaluated for measures of the active and neural stability systems, and analyzed using a canonical correlation analysis, which is used to measure the relationship between 2 multidimensional variables. Results. There were 2 significant relationships identified. The first identified a relationship among fatigue, flexion relation, and the feedforward abdominal response (r = 0.764, P < 0.001). The second relationship was between the Sorenson endurance test and feedforward abdominal response (r = 0.697, P = 0.006). Conclusion. Identification of relationships between back pain measurements may allow discrimination between individuals with different histology and pain levels. This process may assist the specificity of treatment programs prescribed for patients with back pain.
Fast Kinetic Methods with Photodiode Array Detection in the Study of the Interaction and Electron Transfer Between Flavodoxin and Ferredoxin NADP+-Reductase

The primary function of Photosystem I (PSI)1 during photosynthesis is to provide reducing equivalents, in the form of NADPH, that will then be used in CO2 assimilation (Golbeck, 2006). In plants, this occurs via reduction of the soluble [2Fe-2S] Ferredoxin (Fd) by PSI. Subsequent reduction of NADP+ by Fdrd is catalysed by the FAD-dependent FerredoxinNADP+ reductase (FNR) (Eox/hq = -374 mV at pH 8.0 and 25oC) through the formation of a ternary complex (Arakaki et al., 1997; Nogues et al., 2004; Sancho et al., 1990). In most cyanobacteria and some algae under low iron conditions the FMN-dependent Flavodoxin (Fld), particularly its Fldsq/Fldhq couple (Eox/sq = -256 mV, Esq/hq = -445 mV at pH 8.0 and 25oC), substitutes for the Fdox/Fdrd pair in this reaction (Bottin & Lagoutte, 1992; Goni et al., 2009; Medina & Gomez-Moreno, 2004). Thus, two Fldhq molecules transfer two electrons to one FNRox that gets fully reduced after formation of the FNRsq intermediate. FNRhq transfers then both electrons simultaneously to NADP+ (Medina, 2009) (Figure 1). Additionally to their role in photosynthesis, Fld and FNR are ubiquitous flavoenzymes that deliver low midpoint potential electrons to redox-based metabolisms in plastids, mitochondria and bacteria in all kingdoms (Muller, 1991). They are also basic prototypes for a large family of di-flavin electron transferases that display common functional and structural properties, where the electron transfer (ET) flow supported by the Fld/FNR modules occurs in reverse direction to the photosynthesis (Brenner et al., 2008; Wolthers & Scrutton, 2004). The better understanding of the ET flavin-flavin mechanism in this system should witness a greater comprehension of the many physiological roles that Fld and FNR, either free or as modules in multidomain proteins, play. In these chains there are still many
Black-box modeling to estimate tissue temperature during radiofrequency catheter cardiac ablation: feasibility study on an agar phantom model

The aim of this work was to study linear deterministic models to predict tissue temperature during radiofrequency cardiac ablation (RFCA) by measuring magnitudes such as electrode temperature, power and impedance between active and dispersive electrodes. The concept involves autoregressive models with exogenous input (ARX), which is a particular case of the autoregressive moving average model with exogenous input (ARMAX). The values of the mode parameters were determined from a least-squares fit of experimental data. The data were obtained from radiofrequency ablations conducted on agar models with different contact pressure conditions between electrode and agar (0 and 20 g) and different flow rates around the electrode (1, 1.5 and 2 L min−1). Half of all the ablations were chosen randomly to be used for identification (i.e. determination of model parameters) and the other half were used for model validation. The results suggest that (1) a linear model can be developed to predict tissue temperature at a depth of 4.5 mm during RF cardiac ablation by using the variables applied power, impedance and electrode temperature; (2) the best model provides a reasonably accurate estimate of tissue temperature with a 60% probability of achieving average errors better than 5 °C; (3) substantial errors (larger than 15 °C) were found only in 6.6% of cases and were associated with abnormal experiments (e.g. those involving the displacement of the ablation electrode) and (4) the impact of measuring impedance on the overall estimate is negligible (around 1 °C).
Calcium transients in mammalian ventricular muscle.

The calcium-sensitive photoprotein aequorin was microinjected into cells of rat and cat ventricular muscle. During subsequent stimulation of the muscle light emission could be detected and this signal is a function of the intracellular [Ca++]. The time course and amplitude of the intracellular [Ca++] transient occurring during contraction is described. The effects on tension and light emission of changing external [Ca++] and stimulus frequency and of adding adrenaline and caffeine to the bathing solution are described. These results show that changes in external calcium and stimulus frequency alter tension by means of changes in the intracellular [Ca++] whereas adrenaline in addition alters the sensitivity of the contractile system to intracellular [Ca++]. The results also suggest that although a fall in intracellular [Ca++] always precedes relaxation, the time course of the fall of [Ca++] is not generally a rate limiting step in the lime course of relaxation.
Neural evidence for moral intuition and the temporal dynamics of interactions between emotional processes and moral cognition

Behavioral and neurological studies have revealed that emotions influence moral cognition. Although moral stimuli are emotionally charged, the time course of interactions between emotions and moral judgments remains unknown. In the present study, we investigated the temporal dynamics of the interaction between emotional processes and moral cognition. The results revealed that when making moral judgments, the time course of the event-related potential (ERP) waveform was significantly different between high emotional arousal and low emotional arousal contexts. Different stages of processing were distinguished, showing distinctive interactions between emotional processes and moral reasoning. The precise time course of moral intuition and moral reasoning sheds new light on theoretical models of moral psychology. Specifically, the N1 component (interpreted as representing moral intuition) did not appear to be influenced by emotional arousal. However, the N2 component and late positive potential were strongly affected by emotional arousal; the slow wave was influenced by both emotional arousal and morality, suggesting distinct moral processing at different emotional arousal levels.
Fit and Healthy Family Camp for Engaging Families in a Child Obesity Intervention: A Community Health Center Pilot Project

Childhood obesity has become a significant problem in the United States, especially in poor communities, and we know that children who have at least 1 obese parent have a much higher risk of being obese. Combined behavioral lifestyle interventions and family-based intervention programs to address pediatric obesity have the strongest evidence of effectiveness. In this pilot study, we delineate our collaborative model of family group medical visits at a community health center combined with healthy living workshops involving families with at least 1 child and 1 parent or guardian who are obese as a unique approach to address obesity in children.
GABAergic tonic inhibition of substantia gelatinosa neurons in mouse spinal cord

We used whole-cell recording to identify, for the first time, GABAergic tonic current in subpopulations of substantia gelatinosa neurons in mouse spinal cord slices. Application of the &ggr;-aminobutyric acid type A receptor antagonist bicuculline revealed tonic currents in 49 of 73 substantia gelatinosa neurons. These tonic currents were dissected into three groups according to their diverse pharmacological properties. In the first group, furosemide failed to block the tonic current while midazolam and propofol potentiated it. In the second group, furosemide blocked the tonic current but midazolam and propofol failed to enhance the current. In the third group, furosemide blocked and 3&agr;, 21-dihydroxy-5&agr;-pregnan-20-one enhanced the tonic current. The presence of mRNAs for &agr;4, &agr;5, δ and ϵ subunits in the substantia gelatinosa (reverse-transcriptase polymerase chain reaction) suggest that &ggr;-aminobutyric acid type A receptors comprising &agr;5&bgr;x&ggr;2, &agr;x&bgr;xϵ and &agr;4&bgr;xδ subunits are responsible for tonic currents.
Incidence of Polysaccharide Storage Myopathy: Necropsy Study of 225 Horses

Muscle samples were obtained at necropsy from 225 horses and ponies 1 year of age or older. Samples were processed in routine manner and were stained with hematoxylin and eosin and with periodic acid-Schiff for glycogen. Sections were examined for abnormal glycogen content and amylase- resistant complex polysaccharide and for chronic myopathic change (excessive fiber size variation, increase in number of internal nuclei). A total of 101 horses and ponies with lesions of polysaccharide storage myopathy were identified. Age of affected horses ranged from one to 30 years, with a mean of 14.7 years. Mean age of nonaffected horses was 12 years. Incidence of polysaccharide storage myopathy varied depending on breed; Thoroughbreds had the lowest (27%) and draft-related horses had the highest (86%) incidence. Chronic myopathic changes were more severe in polysaccharide storage myopathy-affected horses than in nonaffected horses. Results of this study indicate that polysaccharide storage myopathy is a common disorder of many breeds of horses and ponies.
Progesterone and estrogen regulation of rat decidual cell expression of proliferating cell nuclear antigen.

This study was an examination of the role of progesterone (P4) and estradiol-17beta (E2) as stromal cell mitogens in the decidua basalis (DB) of the rat during pregnancy. Pregnant rats were ovariectomized (Ovx) on Days 8 and 12 of pregnancy, treated with P4, E2, or both, and killed on Days 10 and 14, which correspond to times of stromal cell proliferation and regression, respectively. In some experiments, rats received pellets of the anti-progestin RU-486 on Day 9 and were killed 6, 12, and 24 h later. The mitotic index (MI) and in situ image analysis of expression of proliferating cell nuclear antigen (PCNA) were used to assess cell cycle progression. Highest expression of PCNA occurred on Days 8-12 of pregnancy, and MI was maximum; MI became zero and PCNA expression decreased dramatically thereafter (i.e., Days 14, 17, 21). Percentage of cells expressing intense PCNA on Day 10 (40%) declined to 5% after Ovx and Ovx + E2 (p < 0.05), whereas Ovx + P4 maintained PCNA. By Day 14, only 1% of stromal cells expressed intense PCNA, which was not significantly altered by Ovx, Ovx + E2, or Ovx + P4 but increased after Ovx + P4 and E2 (p < 0.05). By 6 h of RU-486, MI declined 3-fold, and intense PCNA expression was essentially lost. These changes preceded loss of histological integrity of the DB. Cells with undetectable PCNA steadily increased from 8% at 6 h to 28% by 24 h (p < 0.05). Thus RU-486 appeared to block cell cycle progression and enhanced PCNA turnover. P4 was essential for stromal cell proliferation during early pregnancy (Days 8-10), but this action was lost by Day 14.
Extracorporeal shock wave therapy for plantar fasciitis: randomised controlled multicentre trial

Objective To determine the effectiveness of extracorporeal shock wave therapy compared with placebo in the treatment of chronic plantar fasciitis. Design Randomised, blinded, multicentre trial with parallel group design. Setting Nine hospitals and one outpatient clinic in Germany. Participants 272 patients with chronic plantar fasciitis recalcitrant to conservative therapy for at least six months: 135 patients were allocated extracorporeal shock wave therapy and 137 were allocated placebo. Main outcome measures Primary end point was the success rate 12 weeks after intervention based on the Roles and Maudsley score. Secondary end points encompassed subjective pain ratings and walking ability up to a year after the last intervention. Results The primary end point could be assessed in 94% (n=256) of patients. The success rate 12 weeks after intervention was 34% (n=43) in the extracorporeal shock wave therapy group and 30% (n=39) in the placebo group (95% confidence interval - 8.0% to 15.1%). No difference was found in the secondary end points. Few side effects were reported. Conclusions Extracorporeal shock wave therapy is ineffective in the treatment of chronic plantar fasciitis.
Protective role of complement in experimental Escherichia coli endocarditis

Fourteen strains of Escherichia coli were tested for ability to cause infective endocarditis in rabbits prepared by prior placement of an intracardiac catheter. Strains that were resistant to the bactericidal action of serum caused E. coli endocarditis in 91.4% of rabbits, whereas serum-sensitive strains usually failed to cause persisting infection (11.3% infected, P less than 0.001). Although serum-sensitive E. coli lodged on heart valves within 1 h after intravenous injection, they survived less than 24 h in most normal rabbits. In contrast to normals, all five C6-deficient rabbits injected with a serum-sensitive strain of E. coli developed infective endocarditis (P less than 0.005). No correlation was found between the presence of K1 antigen and the incidence of experimental E. coli endocarditis. Thus, the ability of strains of E. coli to establish persisting endocardial infection in rabbits appears to be directly associated with resistance to the complement-mediated serum bactericidal system. These findings may explain in part the rarity of gram-negative bacillary endocarditis in patients; they also indicate that in certain special circumstances the serum bactericidal system can play a decisive role in host defense.
Influence of saline infusion on blood-tissue albumin transport.

Anesthetized rats were infused with lactated Ringer solution (LR) at constant rate for 30 or 60 min; delivered volume loads ranged from 0.03 to 0.08 ml/g body wt. Controls were given only a sustaining infusion of saline at 0.002 ml.g-1.h-1. Only 7-14% of the LR remained in the plasma at the end of the infusion; 76-88% entered the interstitial compartment, and 7-17% was excreted. The amount of plasma protein lost from the circulation with the extravasated fluid was studied simultaneously by two methods: 1) material balance in the whole animal and 2) changes in 131I-labeled albumin uptake (VA) and water content (VW) in individual tissues. The extravasation of 0.03-0.06 ml fluid/g body wt (75-160% initial plasma volume) did not significantly increase plasma protein extravasation in the whole rat. Nearly all of the sampled tissues of LR-infused rats had higher VW than controls. Tissue VA tended to increase with VW, but the regression slopes (delta VA/delta VW), a measure of the tracer albumin concentration of capillary filtrate relative to plasma, were low; skin, 0.006; paw, 0.018; skeletal muscles, 0.007; heart, 0.057; jejunum, 0.095; ileum, 0.045; cecum, 0.026; and colon, 0.027. These ratios are consistent with the very small loss of total plasma protein observed and attest to high solvent-drag reflection coefficients (sigma approximately equal to 1 - delta VA/delta VW): greater than 0.98 in capillaries of skeletal muscles, skin, and paw and 0.91-0.97 in heart and intestine.
Exploiting the Annexin A1 pathway for the development of novel anti‐inflammatory therapeutics

The appreciation that the inflammatory reaction does not ‘spontaneously’ finish, but rather that inflammatory resolution is an active phenomenon brought about by endogenous anti‐inflammatory agonists opens multiple opportunities for a reassessment of the complexity of inflammation and its main mediators. This review dwells on one of these pathways, the one centred around the glucocorticoid‐regulated protein Annexin A1 and its G protein‐coupled receptor. In recent years, much of the knowledge detailing the processes by which Annexin A1 expresses its anti‐inflammatory role on innate immunity has been produced. Moreover, the generation of the Annexin A1 null mouse colony has provided important proof‐of‐concept experiments demonstrating the inhibitory properties of this mediator in the context of inflammatory and/or tissue‐injury models. Therefore, Annexin A1 acts as a pivotal homeostatic mediator, where if absent, inflammation would overshoot and be prolonged. This new understanding scientific information could guide us onto the exploitation of the biological properties of Annexin A1 and its receptor to instigate novel drug discovery programmes for anti‐inflammatory therapeutics. This line of research relies on the assumption that anti‐inflammatory drugs designed upon endogenous anti‐inflammatory mediators would be burdened by a lower degree of secondary effects as these agonists would be mimicking specific pathways activated in our body for safe disposal of inflammation. We believe that the next few years will produce examples of such new drugs and the validity of this speculation could then be assessed.
Retired status and older adults' 10-year drinking trajectories.

OBJECTIVE Little research has examined the role of retirement in shaping late-life drinking careers, and it has generally been limited to cross-sectional designs or short-term follow-ups that emphasize group-level comparisons of retirees and nonretirees. The purpose of this study was to determine the following: (a) the effect of retired status on older adults' 10-year within-person drinking trajectories and (b) whether age, gender, income, health, and problem-drinker status account for or moderate this effect. METHOD We first estimated older adults' (baseline M= 62 years; n = 595) 10-year within-person drinking trajectories using three successively predictive multilevel regression models: unconditional growth, retired status alone, and retired status controlling for covariates. Next, we determined whether inclusion of Retired Status x Covariate interactions would improve prediction of the trajectories. RESULTS Participants' drinking frequency declined moderately over the 10-year interval, and retired status hastened the decline. However, this effect disappeared once covariates were added to the model: Baseline poorer health, lower income, and current problem-drinker status predicted steeper decline in drinking frequency, whereas former problem-drinker status predicted slower decline. Lower income and current drinking problems also predicted steeper declines in amount of alcohol consumed. There were no statistically significant or uniquely contributive interactions between retired status and age, gender, health, income, or drinking problems for predicting late-life drinking trajectories. CONCLUSIONS Baseline health, income, and problem-drinking history are more important than retired status for predicting older adults' long-term within-person drinking trajectories. These factors-and recency of drinking problems-should be considered in future studies of retirement and late-life drinking patterns.
Induction of CspA, an E. coli major cold-shock protein, upon nutritional upshift at 37 degrees C.

BACKGROUND The synthesis of CspA, the major cold-shock protein of Escherichia coli, is dramatically induced upon cold shock. It was recently reported that there is massive presence of CspA under nonstress conditions, and it is thus claimed that CspA as the cold-shock protein is a misnomer. RESULTS Here, we re-examined and confirmed that CspA is induced upon culture dilution at 37 degrees C. However, its induction level is one-sixth of the cold-shock-induced level, clearly indicating that the major stress that induces CspA is cold shock. It was further found that CspA induction can be achieved not only by culture dilution but also by the simple addition of nutrients, and that it was almost completely abolished in the presence of rifampicin or nalidixic acid. Nutritional upshift causes the induction of only CspA but not other cold-shock-inducible CspA homologues. The amount of cspA mRNA rapidly and transiently increased by culture dilution, but its stability was not significantly changed. CONCLUSIONS These results suggest that CspA is a nutritional-upshift stress protein as well as a cold-shock stress protein, and that CspA induction following nutritional upshift may be due to transcriptional activation.
Antidepressant Prescription Medication Use Patterns among Insured, Low Income Pregnant Women

Despite of the controversy of antidepressant use during the gestational period, the reported antidepressant use at any time during pregnancy has increased. Our study assessed antidepressant prescription medication use patterns during pregnancy among insured, low-income women in the United States. This retrospective study used South Carolina Medicaid claims data (2004-2009). Included were pregnant women (≥ 18 years) with single or multiparous live birth(s) with a diagnosis for major depression or reported pharmacy claims for antidepressant prescriptions 1 year before becoming pregnant or while pregnant. Antidepressant prevalence prior to and during pregnancy, type of antidepressant, and trimester when the antidepressant use started, characterized antidepressant prescription use patterns. Among 24,077 pregnant women with live births, the prevalence of antidepressant use before becoming pregnant and during pregnancy was 5.5% and 6% respectively. A total of 3,237 pregnant women met inclusion criteria. Of the 1,456 women prescribed antidepressants during pregnancy, 16% showed a history of major depression predating their pregnancy and 67% initiated antidepressant medications after becoming pregnant. Nearly 80% took selective serotonin reuptake inhibitors (SSRIs) during pregnancy, and 32% initiated antidepressants during the first trimester. Age, race, and preexisting depression were associated with antidepressant use. In pregnant, insured low-income women, the majority prescribed antidepressants were new users. Pharmacologic treatment for pregnant women with depressive symptoms, particularly in newly diagnosed major depression remains challenging.
Granulopoiesis in chronic myeloid leukaemia. I. In vitro cloning of blood and bone marrow cells in agar culture.

Peripheral blood and bone marrow cells from patients with chronic myeloid leukaemia were grown in agar culture. The number of colonies grown from the blood was 1,000–60,000 times greater than in normal controls. Some correlations was found between the concentration of leucocytes and colony forming cells of the blood. The autostimulation found in some cases might indicate increased production of the colony stimulating factor in chronic myeloid leukaemia. Mature granulocytes from both healthy and leukaemic donors inhibited to about the same degree colony formation from low density marrow cells of control donors also in the, presence of a feeder layer of mononuclear blood leucocytes. It is possible that a control mechanism of granulopoiesis involves feed back inhibition by substances produced by mature granulocytes. In a few cases of chronic myeloid leukaemia the colony forming cells seemed to be resistant to the inhibitory action of mature granulocytes.