Miracle-dz/UniFAR-FTUs huggingface dataset:
Changes in the Chondrocyte and Extracellular Matrix Proteome during Post-natal Mouse Cartilage Development*

Skeletal growth by endochondral ossification involves tightly coordinated chondrocyte differentiation that creates reserve, proliferating, prehypertrophic, and hypertrophic cartilage zones in the growth plate. Many human skeletal disorders result from mutations in cartilage extracellular matrix (ECM) components that compromise both ECM architecture and chondrocyte function. Understanding normal cartilage development, composition, and structure is therefore vital to unravel these disease mechanisms. To study this intricate process in vivo by proteomics, we analyzed mouse femoral head cartilage at developmental stages enriched in either immature chondrocytes or maturing/hypertrophic chondrocytes (post-natal days 3 and 21, respectively). Using LTQ-Orbitrap tandem mass spectrometry, we identified 703 cartilage proteins. Differentially abundant proteins (q < 0.01) included prototypic markers for both early and late chondrocyte differentiation (epiphycan and collagen X, respectively) and novel ECM and cell adhesion proteins with no previously described roles in cartilage development (tenascin X, vitrin, Urb, emilin-1, and the sushi repeat-containing proteins SRPX and SRPX2). Meta-analysis of cartilage development in vivo and an in vitro chondrocyte culture model (Wilson, R., Diseberg, A. F., Gordon, L., Zivkovic, S., Tatarczuch, L., Mackie, E. J., Gorman, J. J., and Bateman, J. F. (2010) Comprehensive profiling of cartilage extracellular matrix formation and maturation using sequential extraction and label-free quantitative proteomics. Mol. Cell. Proteomics 9, 1296–1313) identified components involved in both systems, such as Urb, and components with specific roles in vivo, including vitrin and CILP-2 (cartilage intermediate layer protein-2). Immunolocalization of Urb, vitrin, and CILP-2 indicated specific roles at different maturation stages. In addition to ECM-related changes, we provide the first biochemical evidence of changing endoplasmic reticulum function during cartilage development. Although the multifunctional chaperone BiP was not differentially expressed, enzymes and chaperones required specifically for collagen biosynthesis, such as the prolyl 3-hydroxylase 1, cartilage-associated protein, and peptidyl prolyl cis-trans isomerase B complex, were down-regulated during maturation. Conversely, the lumenal proteins calumenin, reticulocalbin-1, and reticulocalbin-2 were significantly increased, signifying a shift toward calcium binding functions. This first proteomic analysis of cartilage development in vivo reveals the breadth of protein expression changes during chondrocyte maturation and ECM remodeling in the mouse femoral head.
THE EFFECT OF STRATEGIC LEADERSHIP AND ROLE CONFLICT TO EMPLOYEE’S COMMITMENTS WITH WORK STRESS AS MEDIATION

The purpose of this research is to analyze the impact of strategic leadership and role conflict on employee commitment with job stress as mediation. The study used 60 samples of employees from BPR Nusamba Ngunut Tulungagung as respondents. Research data were obtained through questionnaires and analyzed through SmartPLS. The results show significance for the impact of strategic leadership to job stress, role conflict to job stress, role conflict to employee commitment, job stress to employee commitment, and insignificant results on the influence of strategic leadership to employee commitment. Job stress mediates the influence of strategic leadership and role conflict to employee commitment.
Development of Thyroid-Stimulating Hormone Beta Subunit-Producing Cells in the Chicken Embryonic Pituitary Gland

In previous studies, the distribution of thyrotropes in the chicken pituitary gland has been analyzed by immunohistochemistry using heterologous antibodies. In this study, we examined the distribution of thyroid-stimulating hormone beta subunit-immunopositive (TSHbeta-ip) cells and the expression of TSHbeta mRNA in the pituitary glands of chicken embryos by immunohistochemistry using a specific antiserum to the chicken TSHbeta, in situ hybridization and RT-PCR. Immunohistochemical and morphometric analyses revealed that the TSHbeta-ip cells first appeared on embryonic day 10 (E10) in the pituitary gland and were mainly distributed in the cephalic lobe and that the cell density on E20 was almost 4 times greater than that on E10. The chicken TSHbeta-ip cells could be classified into two types based on morphological characteristics: round-shaped cells and club-shaped cells, which have long cytoplasmic processes. In situ hybridization analysis revealed that TSHbeta mRNA-expressing cells were expressed from E9 in the cephalic lobe and that the extent of TSHbeta mRNA-expressing cells coincided with that of TSHbeta-ip cells. RT-PCR also showed that TSHbeta mRNA was expressed from E9 and that Pit-1 mRNA was expressed from E5. These results clearly demonstrated that the expression of chicken TSHbeta mRNA starts on E9, that TSHbeta-ip cells appear on E10, mainly in the cephalic lobe, and that TSHbeta-ip cells can be classified into two cell types (round- and club-shaped cells).
An integrative approach to the study of Helicotylenchus (Nematoda: Hoplolaimidae) Colombian and Brazilian populations associated with Musa crops

Abstract In total, 10 populations of Helicotylenchus associated with Musa spp. , six from Colombia, and four from Brazil were identified to the species level. Morphological and morphometric data were recorded for each population, performed a principal components analysis (PCA), a conglomerate, along with consensus sequences of D2 to D3 expansion segment of the large subunit of ribosomal DNA (28S) for different populations. Identify of species were performed using the basic local alignment search tool (BLAST), and the evolutionary relationships of species were explored using a phylogeny study. Four species of Helicotylenchus were identified based on morphological and morphometric data: H. multicinctus, H. dihystera, H. erythrinae, and H. californicus. PCA and conglomerate analysis clearly separated these species. BLAST and phylogenetic analysis confirmed the presence of these species associated with Musa spp. crops. This is the first report of these species in Colombia through integrative taxonomy.
Critical exponents of the fully frustrated two-dimensional XY model.

We present a detailed study of the critical properties of the two-dimensional (2D) XY model with maximal frustration in a square lattice. We use extensive Monte Carlo simulations to study the thermodynamics of the spin and chiral degrees of freedom, concentrating on their correlation functions. The gauge-invariant spin-spin correlation functions are calculated close to the critical point for lattice sizes up to 240\ifmmode\times\else\texttimes\fi{}240; the chiral correlation functions are studied on lattices up to 96\ifmmode\times\else\texttimes\fi{}96. We find that the critical exponents of the spin-phase transition are \ensuremath{\nu}=0.3069 and \ensuremath{\eta}=0.1915, which are to be compared with the unfrustrated XY model exponents \ensuremath{\nu}=1/2 and \ensuremath{\eta}=0.25. We also find that the critical exponents of the chiral transition are ${\ensuremath{\nu}}_{\mathrm{\ensuremath{\chi}}}$=0.875, 2\ensuremath{\beta}=0.1936, 2\ensuremath{\gamma}=1.82, and 2\ensuremath{\gamma}'=1.025, which are different from the expected 2D Ising critical exponents. The spin-phase transition occurs at ${\mathit{T}}_{\mathit{U}(1)}$=0.446, which is about 7% above the estimated chiral critical temperature ${\mathit{T}}_{\mathit{Z}2}$=0.4206. However, because of the size of the statistical errors, it is difficult to decide with certainty whether the transitions occur at the same or at slightly different temperatures. Finally, the jump in the helicity modulus in the fully frustrated system is found to be about 23% below the unfrustrated universal value. The most important consequence of these results is that the fully frustrated XY model appears to be in a novel universality class. Recent successful comparisons of some of these results with experimental data are also briefly discussed.
Pediatric End-of-life Care Barriers and Facilitators: Perception of Nursing Professionals in Jordan

Introduction: End-of-life care is a critical issue for pediatric population with terminal illness to ensure the best possible quality of care for them and their families. A survey was conducted to identify the barriers and facilitators to provide pediatric end-of-life care. Methods: A descriptive cross-sectional study was conducted at three tertiary centers providing end-of-life care in Jordan. Two hundred critical care nurses were surveyed (response rate 93%). Results: Nurses reported moderate level of experience in all areas of delivering pediatric end-of-life care. The highest scoring of barriers respectively were patients-families barriers having deal with angry family member; health-care professional barriers multiple physicians, involved with one patient, who differ in opinion about the direction care, and where plan of care should go; and organizational barriers not available support person for the family. The highest scoring of facilitators respectively were of patients families facilitators having family members accept that the patient is dying; health-care professional-facilitators having a physician agrees about the direction of care, and organizational facilitators providing family members adequate time to be alone with the pediatric after he or she has died. Conclusion: Nurses perceived that patient-family, health-care professionals, and organizational related barriers and facilitators were had the most influence in providing of pediatric end-of-life care. Findings highlighted the need for additional education and support for pediatric staff, across professions, in providing pediatric end-of-life care. A pediatric end-of-life care team should be developed to assist in improving patients' quality of care and increasing the awareness for the need for a standardized tool to evaluate the nursing competency level concerning pediatric end-of-life care.
The Barcelona approach: Diagnosis, staging, and treatment of hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is the fifth most common neoplasm in the world, and the third most common cause of cancer‐related death. It affects mainly patients with cirrhosis of any etiology. Patients with cirrhosis are thus usually included in surveillance plans aiming to achieve early detection and effective treatment. Only patients who would be treated if diagnosed with HCC should undergo surveillance, which is based on ultrasonography and α‐fetoprotein every 6 months. Upon diagnosis, the patients have to be staged to define tumor extent and liver function impairment. Thereafter, the best treatment option can be indicated and a prognosis estimate can be established. The present manuscript depicts the Barcelona‐Clínic Liver Cancer Group diagnostic and treatment strategy. This is based on the analysis of several cohort and randomized controlled studies that have allowed the continuous refinement of treatment indication and application. Surgical resection is considered the first treatment option for early stage patients. It is reserved for patients with solitary tumors without portal hypertension and normal bilirubin. If these conditions are not met, patients are considered for liver transplantation (cadaveric or live donation) or percutaneous ablation if at an early stage (solitary ≤ 5 cm or up to 3 nodules ≤ 3 cm). These patients will reach a 5‐year survival between 50 and 75%. If patients are diagnosed at an intermediate stage and are still asymptomatic and have preserved liver function, they may benefit from chemoembolization. Their 3‐year survival will exceed 50%. There is no effective treatment for patients with advanced disease and thus, in such instances, the patients have to be considered for research trials with new therapeutic options. Finally, patients with end‐stage disease should receive only palliative treatment to avoid unnecessary suffering. (Liver Transpl 2004;10:S115–S120.)
Comparison of Monotherapy Versus Combination Antihypertensive Therapy in Elderly Patients With Essential Hypertension

Authors sought to compare the efficacy of monotherapy versus combination antihypertensive therapy in elderly patients. Patients in this study, aged 65 to 85 years, were divided into 4 groups and entered an 8-week treatment period. First group: 22 patients, amlodipine 5 mg/d increasing to 10 mg; second: 20 patients, eprosartan 600 mg/d increasing to 600 mg twice a day; third: 21 patients, amlodipine 5 mg/d and indapamide 2.5 mg/d, increasing amlodipine to 10 mg/d; fourth: 23 patients, imidapril 10 mg/d and indapamide 2.5 mg/d, imidapril doubled to 20 mg/d. A greater drop in systolic and in diastolic blood pressure was obtained by combination of amlodipine and indapamide compared with amlodipine or eprosartan monotherapy. Imidapril and indapamide showed similar efficacy compared with eprosartan monotherapy but not with amlodipine monotherapy. Amlodipine and indapamide appeared more effective than imidapril and indapamide in diastolic blood pressure. Combination treatment with amlodipine and indapamide or imidapril and indapamide effectively reduces blood pressure in elderly patients with essential hypertension.
Differential Ly49e Expression Pathways in Resting versus TCR-Activated Intraepithelial γδ T Cells

The Ly49 NK receptor family in mice is composed of several members that recognize MHC class I (MHC-I) or MHC-I–related molecules. We and others have shown before that Ly49E is a unique member, with a different expression pattern on NK cells and being triggered by the non–MHC-I–related protein urokinase plasminogen activator. Among the entire Ly49 receptor family, Ly49E is the only Ly49 member expressed by epidermal-localized γδ T cells and their fetal thymic TCRγδ precursors, and it is the most abundantly expressed member on intestinal intraepithelial γδ T cell lymphocytes. In this study, we provide mechanistic insights into the regulation of Ly49e expression in γδ T cells. First, we demonstrate that TCR-mediated activation of intraepithelial γδ T cells significantly increases Ly49E expression. This results from de novo Ly49E expression and is highly selective, because no other Ly49 family members are induced. TCR-mediated Ly49E induction is a conserved feature of skin- and gut-residing intraepithelial-localized γδ T cell subsets, whereas it is not observed in spleen γδ T cells. By investigating Ly49e promoter activities and lymphotoxin (LT) αβ dependency in resting versus TCR-activated intraepithelial γδ T cells, we reveal two separate regulatory pathways for Ly49E expression, as follows: a LTαβ-dependent pathway leading to basal Ly49E expression in resting cells that is induced by Pro2-mediated Ly49e transcription, and a LTαβ-independent pathway leading to elevated, Pro3-driven Ly49E expression in TCR-stimulated cells.
Abnormal Audit Fees and Earnings Management Using Classification Shifting

Prior research has intensively debated whether abnormal audit fees purely indicate audit effort or client-auditor economic bonding (Francis 2011). However, this empirical debate is mostly based on the findings of the associations between audit fees and accrual-based earnings management. Unlike accrual-based earnings management, earnings management through classification shifting does not change the bottom-line numbers and thus involves lower litigation costs. Given the difference in litigation costs between the two forms of earnings management, the effect of abnormal audit fees on the incentives of auditor in dealing with the behavior of earnings management could be different. It is thus interesting to examine how audit fees affect auditors’ incentives on their clients’ earnings management with potential lower litigation risks. Using data from years 2000-2010, we find a significant and positive cross-sectional association between the magnitude of abnormal audit fees and the level of classification shifting, a result supporting the notion that greater abnormal audit fees allow for more earnings management through classification shifting. This observed result further indicates that using abnormal audit fees to purely measure audit effort or economic bonding might be questionable as the effect of abnormal audit fees on opportunistic accounting practices could differ, depending on the specific form of earnings management activities associated with the level of potential litigation costs.
Policy Brief on Women’s Entrepreneurship

This policy brief on women in entrepreneurship was produced by the OECD and the European Commission. It explores the gender gap in entrepreneurship, the differences between male- and female-owned businesses, and the unique challenges that female entrepreneurs face when it comes to starting a business. Policy makers can support female entrepreneurs by promoting a positive attitude and female role models, offering training courses and mentoring, facilitating access to finance, and ensuring that their policies help women to participate in the labour market. This policy brief is part of a series of documents produced by the OECD and the European Commission on inclusive entrepreneurship. The series includes policy briefs on youth entrepreneurship, senior entrepreneurship, social entrepreneurship, evaluation of inclusive entrepreneurship programmes, access to business start-up finance for inclusive entrepreneurship and entrepreneurship by the disabled as a well as a report on ‘The Missing Entrepreneurs’. All these documents are available in English, French and German. They are available at http://www.oecd. org/cfe/leed/inclusive-entrepreneurship.htm.
Current trends and future implications in the developing role of clinical skills centres

Clinical skills centres (CSCs) have contributed significantly to undergraduate medical education. Using alternative venues for training in communication skills, clinical examination and practical procedures they avoid jeopardising patient-care in traditional clinical settings. Using simulation they enable tutors to structure learning opportunities, provide standardized and reproducible experiences and create learner-centred environments where mistakes are permissible. However, recent changes in patients' attitudes and expectations and in students' and doctors' needs indicate that further changes in the delivery and learning of clinical skills are required. Four current trends in teaching and learning are emerging in CSCs. The range of delivery methods available is expanding; educational strategies are being adopted; a variety of assessment opportunities are being identified and the need for support mechanisms for students and tutors is being recognized. The implementation of these trends gives rise to a number of resource implications that must be met. Appropriate planning is required to ensure that the future contribution of CSCs will be successful in training healthcare professionals for practice in contemporary society.
Correlates of smoking before, during, and after pregnancy.

OBJECTIVES To use recent, national data to examine correlates of smoking before, during, and after pregnancy; quitting smoking during pregnancy; and relapsing by 24 months postpartum. METHODS Multivariate logistic regression models used data from the Early Childhood Longitudinal Study-Birth Cohort and accounted for relevant socio-demographic, clinical, and contextual covariates. RESULTS Although socio-demographic characteristics including education, income, race/ethnicity, and marital status were associated with smoking at all time points, contextual factors including alcohol consumption, breast-feeding, and living with other smokers were also strongly associated with smoking, quitting, and relapsing. CONCLUSIONS Results of this study may facilitate the creation of more targeted interventions focusing on women at highest risk for smoking during this critical period.
Aging Gut Microbiota at the Cross-Road between Nutrition, Physical Frailty, and Sarcopenia: Is There a Gut–Muscle Axis?

Inadequate nutrition and physical inactivity are the mainstays of primary sarcopenia–physiopathology in older individuals. Gut microbiota composition is strongly dependent on both of these elements, and conversely, can also influence the host physiology by modulating systemic inflammation, anabolism, insulin sensitivity, and energy production. The bacterial metabolism of nutrients theoretically influences skeletal muscle cell functionality through producing mediators that drive all of these systemic effects. In this study, we review the scientific literature supporting the concept of the involvement of gut microbiota in primary sarcopenia physiopathology. First, we examine studies associating fecal microbiota alterations with physical frailty, i.e., the loss of muscle performance and normal muscle mass. Then, we consider studies exploring the effects of exercise on gut microbiota composition. Finally, we examine studies demonstrating the possible effects of mediators produced by gut microbiota on skeletal muscle, and intervention studies considering the effects of prebiotic or probiotic administration on muscle function. Even if there is no evidence of a distinct gut microbiota composition in older sarcopenic patients, we conclude that the literature supports the possible presence of a “gut–muscle axis”, whereby gut microbiota may act as the mediator of the effects of nutrition on muscle cells.
Involvement of the rat prefrontal cortex in a delayed reinforcement operant task

To determine whether the rat medial prefrontal cortex (PFC) is involved in delayed reinforcement operant behavior, we studied the effects of transient inactivation of the medial PFC (or the hippocampus as a control) during a delayed reinforcement lever-press task. We demonstrated the involvement of the PFC in this task: PFC inactivation but not hippocampal inactivation significantly impaired performance. In a separate experiment, we also recorded the prefrontal multiple unit activities during the task to determine the roles of the PFC in detail. Neuronal activity decreased during the delay period, suggesting that this decrease plays a role in delayed reinforcement operant behavior.
Don't look back: Retroactive, dynamic costs and benefits of emotional capture

When people search for targets within rapid streams of images, irrelevant emotional distractors—relative to neutral distractors—spontaneously demand attention and impair subsequent target detection, an effect that can be likened to an emotion-induced “attentional blink”. But what happens when emotional distractors appear after a target has already come and gone? Here, we describe new findings of retroactive emotion-induced effects on target awareness. First, emotion-induced impairments of target awareness extended even to targets that appeared immediately before emotional distractors (Experiment 1). Second, when targets preceded distractors by two items—rather than by one item—negative distractors led to enhanced target processing relative to when distractors were neutral (Experiment 2). In contrast, when a target appeared after an emotional distractor, target awareness was impaired regardless of whether it was the first or second subsequent item. These results potentially implicate separable impacts of emotion on target processing, which can be distinguished by their facilitatory versus disruptive effects and by their temporal dynamics.