Miracle-dz/UniFAR-FTUs huggingface dataset:
Liver transplantation following donation after cardiac death: An analysis using matched pairs

Grafts from donation after cardiac death (DCD) donors are used to increase the number of organs available for liver transplantation. There is concern that warm ischemia may impair graft function. We compared our DCD recipients with a case‐matched group of donation after brain death (DBD) recipients. Between January 2002 and April 2008, 39 DCD grafts were transplanted. These were matched with 39 DBD recipients on the basis of identified variables that had a significant impact on mortality. These were used to individually match DCD and DBD patients with similar predictive mortality. We compared patient/graft survival, primary non‐function (PNF), and rates of complications. Of all liver transplants, 6.1% were DCD grafts. PNF occurred twice in the DCD group. The incidence of nonanastomotic biliary strictures (NABS; 20.5% versus 0%, P = 0.005) and hepatic artery stenosis (HAS; 12.8% versus 0%, P = 0.027) in the DCD group was higher. One‐year (79.5% versus 97.4%, P = 0.029) and 3‐year (63.6% versus 97.4%, P = 0.001) graft survival was lower in the DCD group. Three‐year patient survival was also lower (68.2% versus 100%, P < 0.0001). Our study is the first to use case‐matched patients and compare groups with similar predictive mortality. There was a higher incidence of NABS and HAS in the DCD group. NABS were likely a result of warm ischemia. HAS may have been due to ischemia or arterial injury during retrieval. The DCD group had significantly poorer outcomes, but DCD grafts remain a valuable resource. With careful donor/recipient selection, minimization of ischemia, and good postoperative care, acceptable results can be achieved. Liver Transpl 15:1072–1082, 2009. © 2009 AASLD.
C-Terminal Domain of the Epstein-Barr Virus LMP2A Membrane Protein Contains a Clustering Signal

ABSTRACT The latency-regulated transmembrane protein LMP2A interferes with signaling from the B-cell antigen receptor by recruiting the tyrosine kinases Lyn and Syk and by targeting them for degradation by binding the cellular E3 ubiquitin ligase AIP4. It has been hypothesized that this constitutive activity of LMP2A requires clustering in the membrane, but molecular evidence for this has been lacking. In the present study we show that LMP2A coclusters with chimeric rat CD2 transmembrane molecules carrying the 27-amino-acid (aa) intracellular C terminus of LMP2A and that this C-terminal domain fused to the glutathione-S-transferase protein associates with LMP2A in cell lysates. This molecular association requires neither the cysteine-rich region between aa 471 and 480 nor the terminal three aa 495 to 497. We also show that the juxtamembrane cysteine repeats in the LMP2A C terminus are the major targets for palmitoylation but that this acylation is not required for targeting of LMP2A to detergent-insoluble glycolipid-enriched membrane microdomains.
Scheduling Algorithms for Simultaneous Software Updates of Electronic Devices in Vehicles

Today's upper-class passenger cars have various interconnected electronic devices. Each device performs complex functions, enabled by software that can be stored in a flash memory. Of these, the devices in the multimedia and infotainment domain contain by far the most software with a size in the order of one Gbyte. In this domain, the devices are the performance bottlenecks, not the communication systems. Throughout the vehicle life cycle, parts of the software have to be frequently updated during maintenance. Today, the software of the devices is updated in a consecutive manner. Due to performance bottlenecks caused by the affected devices, the update can take a long time that leads to high costs. Therefore, the objective is to reduce the total update time by a higher utilization of the common bus resource. In this paper, we introduce and investigate algorithms that update the software of multiple devices simultaneously and evaluate the efficiency of these algorithms. We focus on scheduling algorithms on the Application layer and the logical link control (LLC) layer and model the update process by means of Petri nets. Our studies show that it is most promising to combine a simple scheduling algorithm on the Application layer with Round Robin on the LLC layer.
Immature HIV-1 lattice assembly dynamics are regulated by scaffolding from nucleic acid and the plasma membrane

Significance In order for HIV to proliferate, viral proteins and genomic dimers are assembled at host cell membranes and released as immature virions. Disrupting this key intermediate step in viral replication is a potential target for treatment. However, a detailed molecular view of this process remains lacking. Here, we elucidate a network of constitutive interactions that regulate viral assembly dynamics through a combined computational and experimental approach. Specifically, our analysis reveals the active roles of nucleic acid and the membrane as scaffolds that promote the multimerization of Gag polyprotein, which proceeds through multistep and self-correcting nucleation. Our findings also illustrate the functional importance of the N-terminal, C-terminal, and spacer peptide 1 protein domains. The packaging and budding of Gag polyprotein and viral RNA is a critical step in the HIV-1 life cycle. High-resolution structures of the Gag polyprotein have revealed that the capsid (CA) and spacer peptide 1 (SP1) domains contain important interfaces for Gag self-assembly. However, the molecular details of the multimerization process, especially in the presence of RNA and the cell membrane, have remained unclear. In this work, we investigate the mechanisms that work in concert between the polyproteins, RNA, and membrane to promote immature lattice growth. We develop a coarse-grained (CG) computational model that is derived from subnanometer resolution structural data. Our simulations recapitulate contiguous and hexameric lattice assembly driven only by weak anisotropic attractions at the helical CA–SP1 junction. Importantly, analysis from CG and single-particle tracking photoactivated localization (spt-PALM) trajectories indicates that viral RNA and the membrane are critical constituents that actively promote Gag multimerization through scaffolding, while overexpression of short competitor RNA can suppress assembly. We also find that the CA amino-terminal domain imparts intrinsic curvature to the Gag lattice. As a consequence, immature lattice growth appears to be coupled to the dynamics of spontaneous membrane deformation. Our findings elucidate a simple network of interactions that regulate the early stages of HIV-1 assembly and budding.
Special Education Today in Ghana

Abstract The Ghana chapter on special education begins with the history of service provisions for persons with disabilities. It includes information on educational and rehabilitation services, special schools and integrated education. Detailed data is related concerning prevalence and incidence rates and special needs among the Ghana population. This is followed by a comprehensive section on regular and special education teacher roles, expectations, and training. An important aspect of Ghana’s special education is its movement towards inclusive education. The support for this movement comes from the Ministry of Education’s policy, namely, The Education Strategic Plan (ESP), which adopts inclusive education and promotes it as the future special education direction for the country. The chapter provides detailed information on the issues related to the implementation of the ESP plan, four models that have been developed for inclusive education, the progress and effort that Ghana has made towards inclusive education as well as significant challenges that are present.
Hepatitis B, hepatitis C, and HIV in correctional populations: a review of epidemiology and prevention

The 2 million persons incarcerated in US prisons and jails are disproportionately affected by hepatitis B virus (HBV), hepatitis C virus (HCV) and HIV, with prevalences of infection two to ten times higher than in the general population. Infections are largely due to sex- and drug-related risk behaviors practised outside the correctional setting, although transmission of these infections has also been documented inside jails and prisons. Public health strategies to prevent morbidity and mortality from these infections should include hepatitis B vaccination, HCV and HIV testing and counseling, medical management of infected persons, and substance abuse treatment in incarcerated populations.
The Efficiency of Equity REIT Prices

Executive Summary. Given the broad array of investment vehicles that investors can choose from in today's financial and capital markets, the knowledge of the efficiency of asset prices and the relative price volatility is essential to informed decision making. The purpose of this research is to determine if real estate investment trust (REIT) prices have been efficient. In order to test the efficiency of REIT prices, two statistical tests were performed—a runs test and an autocorrelation test. The results of both tests suggest a degree of inefficiency in REIT prices.
Campylobacter jejuni Antibodies in Nigerian Children

Titers of complement-fixing (CF) antibody to Campylobacter jejuni were demonstrated in 87 (36.7%) of 237 infants 6 to 15 months old in Jos, Nigeria. Of the total number of children examined, 81 had acute diarrhea and 27 of them (33.3%) were found to have CF antibodies in their serum. The remaining 156 children were asymptomatic, and 60 (38.4%) of them had CF antibodies. In the diarrheal group, 27 of 75 children 6 to 8 months old were CF antibody positive. There was no significant difference in the incidence of CF C. jejuni antibodies in the diarrheal and nondiarrheal infants (P greater than 0.05). Also, infants 9 to 15 months old had a higher incidence of CF antibodies (46.5%) than those 6 to 8 months of age (25%). The data suggest that the infants whose sera were CF antibody positive had had an exposure to C. jejuni. All 33 infants 6 to 8 months of age who had no diarrhea were CF antibody negative.
A novel in vitro model for haematogenous spreading of S. aureus device biofilms demonstrating clumping dispersal as an advantageous dissemination mechanism

Staphylococcus aureus is able to disseminate from vascular device biofilms to the blood and organs, resulting in life‐threatening infections such as endocarditis. The mechanisms behind spreading are largely unknown, especially how the bacterium escapes immune effectors and antibiotics in the process. Using an in vitro catheter infection model, we studied S. aureus biofilm growth, late‐stage dispersal, and reattachment to downstream endothelial cell layers. The ability of the released biofilm material to resist host response and disseminate in vivo was furthermore studied in whole blood and phagocyte survival assays and in a short‐term murine infection model. We found that S. aureus biofilms formed in flow of human plasma release biofilm thromboemboli with embedded bacteria and bacteria‐secreted polysaccharides. The emboli disseminate as antibiotic and immune resistant vehicles that hold the ability to adhere to and initiate colonisation of endothelial cell layers under flow. In vivo experiments showed that the released biofilm material reached the heart similarly as ordinary broth‐grown bacteria but also that clumps to some extend were trapped in the lungs. The clumping dispersal of S. aureus from in vivo‐like vascular biofilms and their specific properties demonstrated here help explain the pathophysiology associated with S. aureus bloodstream infections.
Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle

The nature of the deficit underlying age‐related muscle wasting remains controversial. To test whether it could be due to a poor anabolic response to dietary amino acids, we measured the rates of myofibrillar and sarcoplasmic muscle protein synthesis (MPS) in 44 healthy young and old men, of similar body build, after ingesting different amounts of essential amino acids (EAA). Basal rates of MPS were indistinguishable, but the elderly showed less anabolic sensitivity and responsiveness of MPS to EAA, possibly due to decreased intramuscular expression, and activation (phosphorylation) after EAA, of amino acid sensing/signaling proteins (mammalian target of rapamycin, mTOR; p70 S6 kinase, or p70S6k; eukaryotic initiation factor [eIF]4BP‐1; and eIF2B). The effects were independent of insulin signaling since plasma insulin was clamped at basal values. Associated with the anabolic deficits were marked increases in NFκB, the inflammation‐associated transcription factor. These results demonstrate first, EAA stimulate MPS independently of increased insulin availability; second, in the elderly, a deficit in MPS in the basal state is unlikely; and third, the decreased sensitivity and responsiveness of MPS to EAA, associated with decrements in the expression and activation of components of anabolic signaling pathways, are probably major contributors to the failure of muscle maintenance in the elderly. Countermeasures to maximize muscle maintenance should target these deficits.
Extracting information from flowcharts and contingency statements: the effects of age and practice

Two studies are reported which aim to test the generality of the conclusions drawn from previous literature on the effectiveness of flowcharts with adult participants. In Experiment One secondary school children of different ages took part, and in Experiment Two a group of senior citizens participated. In both experiments, the effectiveness of a flow chart was compared with that of short contingency statements for finding a solution to a complex problem. In Experiment One there was an initial advantage for the flow chart over the contingency statements in terms of speed but this was not the case in Experiment Two. Furthermore, the results showed there to be no significant differences in error rates between the two modes of presentation in both experiments. Participants who were particularly accurate responders did not respond any more slowly (or quickly) in Experiment One. Practice at the task had marked effects. The differences in speed between the two modes of presentation on trial 1 were markedly reduced on trial 2 in both experiments and performance speeded up with practice. Age also played a part. In Experiment One, older children performed more rapidly than younger ones. In Experiment Two, the elderly participants found both formats very difficult to use. It seems that the generally accepted view that flowcharts are superior to contingency statements might not hold for younger participants or for the elderly, especially when accuracy is the main criterion.
Multi-Rate Broadcasting: Analysis and Design of Stateless Algorithms

We look at the problem of network wide broadcast using the multi-rate feature of a wireless ad hoc network. Existing research has primarily focused on achieving minimum latency by construction of minimum weight connected dominating sets (WCDS) based on neighbourhood information. In this paper, we are interested in stateless multi-rate broadcasting algorithms in which nodes determine their broadcasting behaviour based on neighbourhood transmissions. The primary contribution of this paper is that we show how broadcast effectiveness at different data rates are related and how this relationship can be used to optimize algorithm design. We propose three stateless broadcasting algorithms and demonstrate the performance improvements achievable. Our simulation results show that significant benefits can be obtained in terms of minimizing both the number of forwarding nodes as well as the broadcast latency.
Structural higher education reform - design and evaluation: synthesis report

This study analyses how different types of system-level (or ‘landscape’) structural reforms in higher education have been designed and implemented in selected higher education systems. In the 12 case studies that form the core of the project, the researchers examine reforms aimed at: - Increasing horizontal differentiation between different types of higher education institutions (for example reforms to introduce or modify the role of universities of applied science); - Increasing vertical differentiation through increasing or decreasing positional or status differences between higher education institutions (for example, reforms aimed at concentrating research in a limited number of universities) and; - Changing institutional interrelationships between higher education institutions (for example, through mergers, the formation of associations of institutions). In each case, the researchers set out to understand the origins and objectives of the reforms examined, the why they were designed and implemented, the extent to which they achieved their objectives and the factors affecting success or failure. The overall objective is to provide policy makers at the European, national and institutional levels with policy relevant conclusions concerning the design, implementation and evaluation of structural reforms
Hemeoxygenase expression in human placenta and placental bed implies a role in regulation of trophoblast invasion and placental function

The purpose of this study was to examine the expression of hemeoxygenases HO‐1 and HO‐2, which are responsible for the production of carbon monoxide (CO), in the human placenta and placental bed and to determine the role of inhibitors of HO on placental perfusion pressure. We hypothesized that HO is expressed within the placenta and that invading cytotrophoblast cells (CTB) express HO isoforms. The expression of HO‐1 and HO‐2 was studied on placenta and placental bed biopsies, obtained using a transcervical sampling technique, from normal human pregnancies between 8 and 19 wk gestation and at term. In the placenta, HO‐2 immunostaining was prominent in syncytiotrophoblast in the first trimester and reduced toward term (P<0.0005). HO‐2 endothelial immunostaining was weak in the first trimester, but increased by term (P<0.0005). Within the placental bed, HO‐2 was expressed by CTB in cell columns, the cytotrophoblast shell, and cell islands. Both intravascular CTB and interstitial CTB expressed HO‐2. HO‐1 immunostaining was low in the placenta but intense on the CTB within the placental bed. A striking feature was the absence of HO‐1 from the proximal layers of cell columns, with strong expression on the more distal CTB layers of the cell columns. In placental perfusion studies, a significant dose‐dependent increase in perfusion pressure was observed in the presence of zinc protoporphyrin, an inhibitor of HO. These results suggest a role for CO in placental function, trophoblast invasion, and spiral artery transformation. Hemeoxygenase expression in human placenta and placental bed implies a role in regulation of trophoblast invasion and placental function. Lyall, F. , Barber, A. , Myatt, L. , Bulmer, J. N. , Robson, S. C. Hemeoxygenase expression in human placenta and placental bed implies a role in regulation of trophoblast invasion and placental function. FASEB J. 14, 208–219(2000)
Haemoglobin S and C affect the motion of Maurer's clefts in Plasmodium falciparum‐infected erythrocytes

The haemoglobinopathies S and C protect carriers from severe Plasmodium falciparum malaria. We have recently shown that haemoglobin S and C interfere with host‐actin remodelling in parasitized erythrocytes and the generation of an actin network that seems to be required for vesicular protein trafficking from the Maurer's clefts (a parasite‐derived intermediary protein secretory organelle) to the erythrocyte surface. Here we show that the actin network exerts skeletal functions by anchoring the Maurer's clefts within the erythrocyte cytoplasm. Using a customized tracking tool to investigate the motion of single Maurer's clefts, we found that a functional actin network restrains Brownian motion of this organelle. Maurer's clefts moved significantly faster in wild‐type erythrocytes treated with the actin depolymerizing agent cytochalasin D and in erythrocytes containing the haemoglobin variants S and C. Our data support the model of an impaired actin network being an underpinning cause of cellular malfunctioning in parasitized erythrocytes containing haemoglobin S or C, and, possibly, for the protective role of these haemoglobin variants against severe malaria.
Clear cell sarcoma of tendons and aponeuroses

Clear cell sarcoma (CCS) of tendons and aponeuroses (malignant melanoma of soft parts) is a rare melanocytic soft tissue sarcoma. The objective of this study was to determine the clinical features, prognostic factors, and optimal treatment policy for patients with this rare disease.
Differences between questionnaire- and interview-based measures of activities of daily living (ADL) ability and their association with observed ADL ability in women with rheumatoid arthritis, knee osteoarthritis, and fibromyalgia

Objectives: Although self-report based on questionnaire is the common method to obtain information about activities of daily living (ADL) ability in rheumatic diseases, little is known about the relationship between measures of ADL ability based on questionnaire, interview, and observation. The present study examined whether measures of self-reported ADL ability based on questionnaire and interview yielded different results, determined whether the magnitude of the difference varied among women with rheumatoid arthritis (RA), knee osteoarthritis (OA), and fibromyalgia (FM), and investigated the relationships between self-reported and observed ADL ability. Method: The 47 ADL tasks of the ADL taxonomy were used to evaluate self-reported ADL ability based on questionnaire (ADL-Q) and interview (ADL-I), and the Assessment of Motor and Process Skills (AMPS) was used to obtain measures of observed ADL ability. Results: Participants across diagnostic groups reported significantly more ADL ability based on the ADL-Q than on the ADL-I. Moderate correlations were found between the ADL-Q and ADL-I ability measures. Although low to moderate correlations were seen between measures based on the AMPS ADL motor scale and the ADL-Q and ADL-I, respectively, correlations between measures based on AMPS ADL process scale and ADL-Q and ADL-I were generally low. Overall, there was no difference in how the measures based on the two modes of self-report related to the observed ADL ability measures. Conclusion: Measures of self-reported ADL ability based on either questionnaire or interview have limited relationship to each other or to observed performance of ADL tasks.
What Autoassociative Network Models May Tell us about the Neurobiology of Memory

In learning matrix associative memory networks, the choice of threshold value is one of the most significant factors for determining the recall performance. Choice of threshold is especially important for multi-step recall, as each network state is dependent on the prior states. Recently, Gibson and Robinson used a statistical approximation to formalize the dynamics of partially connected recurrent networks. Using this formalism, we evaluate all of the possible thresholding sequences and find the sequence that results in the highest storage capacity. The resulting optimal strategy can be closely approximated by a threshold that is proportional to the activation of the network plus an offset. The performance of a simulated associative memory is shown to match well with the predictions of the theory. This strategy corresponds to one of the simplest putative roles of interneurons which provide a linear output proportional to the total unweighted input from the principal cells.