Miracle-dz/UniFAR-FTUs huggingface dataset:
Are we complying with NICE guidelines on the use of prostaglandin E2 for induction of labour? A survey of obstetric units in the UK

Summary To determine whether obstetric units in the UK comply with the recommendations by the National Institute for Clinical Excellence (NICE) on the maximum doses of intravaginal prostaglandin for induction of labour, a cross-sectional telephone survey of all obstetric units in the UK listed on Dr Foster's website was undertaken. The maximum doses recommended by NICE were exceeded by 86.4% (76/88) and 61.1% (55/90) of units that use intravaginal prostaglandin tablet and intravaginal prostaglandin gel, respectively. Units that use prostaglandin tablets were four times more likely to exceed the recommended maximum dose (OR = 4.03, 95% CI, 1.9 – 8.4), six times more likely to use 50% or more of the recommended maximum dose for nulliparous women (OR = 5.9, 95% CI, 3.1 – 11.0), and six times more likely to use 50% or more of the recommended maximum dose for multiparous women (OR = 6.5, 95% CI, 3.0 – 13.9). A majority of obstetric units in the UK exceed the maximum doses of intravaginal prostaglandin recommended by NICE for induction of labour.
New realizations of CMOS current controlled conveyors with variable current gain and negative input resistance

In this paper two new second-generation current controlled current-conveyors (CCCIIs)-based circuits are proposed. The proposed circuits are variable gain CCCII and CCCII with negative resistance. The design of these circuits is based on a simple CMOS CCCII that has high bandwidth with -3 dB cutoff frequency of 580 MHz. With no signal mirroring used in these configurations, lower harmonic distortion can be achieved. HSPICE simulation results for the proposed circuits are included.
Treatment of essential fatty acid deficiency with dietary triglycerides or phospholipids in a murine model of extrahepatic cholestasis6

Background: Essential fatty acid (EFA) deficiency during cholestasis is mainly due to malabsorption of dietary EFA. Theoretically, dietary phospholipids (PL) may have a higher bioavailability than dietary triglycerides (TG) during cholestasis. We developed murine models for EFA deficiency with and without extrahepatic cholestasis, and compared the efficacy of oral supplementation of EFA as PL or TG. Methods: EFA deficiency was induced in mice by feeding a high-fat EFA-deficient (EFAD) diet. After three weeks on this diet, bile duct ligation (BDL) was performed in a subgroup of mice to establish extrahepatic cholestasis. Cholestatic and noncholestatic EFA-deficient mice continued on the EFAD diet (controls), or were supplemented for three weeks with EFA-rich TG or EFA-rich PL. Fatty acid composition was determined in plasma, erythrocytes, liver and brain. Results: After four weeks of EFAD diet, induction of EFA deficiency was confirmed by a six-fold increased triene/tetraene ratio (T/T-ratio) in erythrocytes of non-cholestatic and cholestatic mice (p<0.001). EFA-rich TG and EFA-rich PL were equally effective in preventing further increase of the erythrocyte T/T-ratio, which was observed in cholestatic and non-cholestatic non-supplemented mice (12and 16-fold the initial value, respectively). In cholestatic mice, EFA-rich PL was superior to EFA-rich TG in decreasing T/T-ratios of liver triglycerides and phospholipids (each p<0.05), and in increasing brain phospholipid concentrations of the long-chain polyunsaturated fatty acids (LCPUFA) docosahexaenoic acid and arachidonic acid (each p<0.05). Conclusions: Oral EFA supplementation in the form of PL is more effective than in the form of TG in increasing LCPUFA concentrations in liver and brain of cholestatic EFA-deficient mice. 112 Chapter 6 proefschrift_def_v010605def. qxp 2-6-2005 1:20 Pagina 112
Effect of Radiofrequency Radiation from Telecommunication Base Stations on Microbial Diversity and Antibiotic Resistance

We aimed to investigate the effects of radiofrequency radiation from telecommunication on bacteria diversity and antibiotic sensitivity of surrounding bacteria micro-flora. In all cases of bacteria isolated, results indicated that radiation majorly influences and selected bacteria of specific morphology (rod-like, gram positive and spore formers) belonging to the genera Bacillus, Clostridium, Corynebacterium and Sporosarcina . Spore formation have been associated with prevention of DNA damage, this suggest that radiation may imposes stress detrimental to various non-spore forming, gram negative bacteria of nonrod morphology. The antibiotic resistance pattern indicated resistance above 50% in three of the ten common antibiotics tested with the highest of 96% recorded against zinnacef, followed by amoxicillin (70%) and ampiclox (56%). The highest resistance recorded was attributed to Bacillus spp while lowest resistance was recorded in Corynebacterium. The two largest groups of isolates; Bacillus and Clostridium have been reported as human pathogens and found to be multidrug resistant. © JASEM
The Management of a Multigenerational Nursing Team With Differing Qualifications: A Qualitative Study

Retiring age is no longer fixed at 39 years of working service by Italian law, so nurses with more than 40 years of service continue to work in the wards, creating a distinct older generation. Moreover, with the closure of nursing colleges and the introduction of university degree courses for nurses, nursing education has changed significantly. This article reports on a study which explored the experiences of chief nurses in the daily management of nursing teams composed of multigenerational nurses who differed in age and qualifications. Husserls' phenomenologic approach was used, interviewing at length a purposeful sample of 10 chief nurses. Five main areas of concern emerged: (a) The generation gaps between peers and team leaders; (b) nomadism versus being settled; (c) conflicting methods of working which result in nothing being accomplished: managing the "flat paradox"; (d) different nursing values, ideals, and visions; and (e) existing generation gaps with the younger staff. The number of younger nurses will increase with time and, as a consequence, the relevance of intergenerational differences must be seriously debated, not only difficulties with age differences but also those relating to nursing education.
Reciprocal white matter alterations due to 16p11.2 chromosomal deletions versus duplications

Copy number variants at the 16p11.2 chromosomal locus are associated with several neuropsychiatric disorders, including autism, schizophrenia, bipolar disorder, attention‐deficit hyperactivity disorder, and speech and language disorders. A gene dosage dependence has been suggested, with 16p11.2 deletion carriers demonstrating higher body mass index and head circumference, and 16p11.2 duplication carriers demonstrating lower body mass index and head circumference. Here, we use diffusion tensor imaging to elucidate this reciprocal relationship in white matter organization, showing widespread increases of fractional anisotropy throughout the supratentorial white matter in pediatric deletion carriers and, in contrast, extensive decreases of white matter fractional anisotropy in pediatric and adult duplication carriers. We find associations of these white matter alterations with cognitive and behavioral impairments. We further demonstrate the value of imaging metrics for characterizing the copy number variant phenotype by employing linear discriminant analysis to predict the gene dosage status of the study subjects. These results show an effect of 16p11.2 gene dosage on white matter microstructure, and further suggest that opposite changes in diffusion tensor imaging metrics can lead to similar cognitive and behavioral deficits. Given the large effect sizes found in this study, our results support the view that specific genetic variations are more strongly associated with specific brain alterations than are shared neuropsychiatric diagnoses. Hum Brain Mapp 37:2833–2848, 2016. © 2016 Wiley Periodicals, Inc.
Period prevalence, risk factors and consequent injuries of falling among the Saudi elderly living in Riyadh, Saudi Arabia: a cross-sectional study

Objectives Approximately 28% to 35% of people aged 65 and over fall each year. The consequent injuries of falls are considered a major public health problem. Falls account for more than half of injury-related hospitalisations among old people. The aim of this study was to measure a 1-year period prevalence of falling among old people in Riyadh, Saudi Arabia. In addition, this study described the most common risk factors and consequent injuries of falls. Setting and participants A cross-sectional survey was carried out in Riyadh, using a convenient sampling. The targeted population were Saudi citizens who were 60 years or above. Over a 6-month period, 1182 individuals were sampled (545 men and 637 women). Results The 1-year prevalence of falling among old Saudis (>=60 years) was 49.9%. Our results show that 74% of the participants who experienced falls had postfall injuries. Old participants who were uneducated and those with middle school certification were associated with falls (adjusted OR (aOR) 1.72; 95% CI 1.15 to 2.56, aOR 1.81; 95% CI 1.15 to 2.85, respectively). Those who live in rented houses had a higher risk of falls. Interestingly, having a caregiver was significantly associated with more falls (aOR 1.39; 95% CI 1.08 to 1.79). However, not using any medications was significantly related to fewer falls. In addition, old individuals using walking aids were more likely to fall than those who did not. Participants who mentioned ‘not having stressors were associated with less frequent falls (aOR 0.62; 95% CI 0.39 to 0.97). Cerebrovascular accidents were strongly associated with falls with an estimated OR of 2.75 (95% CI 1.18 to 6.43). Moreover, osteoporosis, poor vision and back pain were found to be predictors for falls among the elderly. Conclusion 49.9% of elderly Saudis had experienced one or more falls during a 12-month period. Several preventable risk factors could be addressed by routine geriatric assessment. Research on the impact of these risk factors is needed.
Modelling the determinants of 2000 m rowing ergometer performance: a proportional, curvilinear allometric approach

Previous studies have investigated the determinants of indoor rowing using correlations and linear regression. However, the power demands of ergometer rowing are proportional to the cube of the flywheel's (and boat's) speed. A rower's speed, therefore, should be proportional to the cube root (0.33) of power expended. Hence, the purpose of the present study was to explore the relationship between 2000 m indoor rowing speed and various measures of power of 76 elite rowers using proportional, curvilinear allometric models. The best single predictor of 2000 m rowing ergometer performance was power at V̇O2max ()0.28, that explained R2=95.3% in rowing speed. The model realistically describes the greater increment in power required to improve a rower's performance by the same amount at higher speeds compared with that at slower speeds. Furthermore, the fitted exponent, 0.28 (95% confidence interval 0.226–0.334) encompasses 0.33, supporting the assumption that rowing speed is proportional to the cube root of power expended. Despite an R2=95.3%, the initial model was unable to explain “sex” and “weight‐class” differences in rowing performances. By incorporating anaerobic as well as aerobic determinants, the resulting curvilinear allometric model was common to all rowers, irrespective of sex and weight class.
The Relative Importance of Clouds and Sea Ice for the Solar Energy Budget of the Southern Ocean

Abstract The effects of clouds and sea ice on the solar radiation budget are determined for the Southern Ocean around Antarctica between latitudes 50° and 80°S. Distributions of cloud optical depth are used, together with distributions of surface albedo, to estimate the geographical and seasonal variations of shortwave irradiance and cloud radiative forcing at the surface, both for the present climate and for altered surface and cloud conditions. Poleward of 68°S in spring, ice causes a greater reduction of solar energy input to the surface than does cloud. However, in summer the clouds are more important than ice at all latitudes in the Southern Ocean. In the present climate the clouds are optically thicker over open water than over sea ice, suggesting a possible negative feedback if the sea ice area shrinks with climatic warming. Compared to the present climate in spring, removing sea ice results in an increase in irradiance reaching the ocean surface, regardless of the type of cloud remaining. However,.
Health and population effects of rare gene knockouts in adult humans with related parents

Rare gene knockouts in adult humans On average, most people's genomes contain approximately 100 completely nonfunctional genes. These loss-of-function (LOF) mutations tend to be rare and/or occur only as a single copy within individuals. Narasimhan et al. investigated LOF in a Pakistani population with high levels of consanguinity. Examining LOF alleles that were identical by descent, they found, as expected, an absence of homozygote LOF for certain protein-coding genes. However, they also identified many homozygote LOF alleles with no apparent deleterious phenotype, including some that were expected to confer genetic disease. Indeed, one family had lost the recombination-associated gene PRDM9. Science, this issue p. 474 The total loss of protein-coding genes, even those with the potential to confer genetic diseases, can be tolerated. Examining complete gene knockouts within a viable organism can inform on gene function. We sequenced the exomes of 3222 British adults of Pakistani heritage with high parental relatedness, discovering 1111 rare-variant homozygous genotypes with predicted loss of function (knockouts) in 781 genes. We observed 13.7% fewer homozygous knockout genotypes than we expected, implying an average load of 1.6 recessive-lethal-equivalent loss-of-function (LOF) variants per adult. When genetic data were linked to the individuals’ lifelong health records, we observed no significant relationship between gene knockouts and clinical consultation or prescription rate. In this data set, we identified a healthy PRDM9-knockout mother and performed phased genome sequencing on her, her child, and control individuals. Our results show that meiotic recombination sites are localized away from PRDM9-dependent hotspots. Thus, natural LOF variants inform on essential genetic loci and demonstrate PRDM9 redundancy in humans.
Identification of a cytoskeletal protein localized in the myoplasm of ascidian eggs: localization is modified during anural development.

The myoplasm of ascidian eggs is a localized cytoskeletal domain that is segregated to presumptive larval tail muscle cells during embryonic development. We have identified a cytoskeletal protein recognized by a vertebrate neurofilament monoclonal antibody (NN18) which is concentrated in the myoplasm in eggs and embryos of a variety of ascidian species. The NN18 antigen is localized in the periphery of unfertilized eggs, segregates with the myoplasm after fertilization, and enters the larval tail muscle cells during embryonic development. Western blots of one-dimensional and two-dimensional gels showed that the major component recognized by NN18 antibody is a 58 x 10(3) Mr protein (p58), which exists in at least three different isoforms. The enrichment of p58 in the Triton X-100-insoluble fraction of eggs and its reticular staining pattern in eggs and embryos suggests that it is a cytoskeletal protein. In subsequent experiments, p58 was used as a marker to determine whether changes in the myoplasm occur in eggs of anural ascidian species, i.e. those exhibiting a life cycle lacking tadpole larvae with differentiated muscle cells. Although p58 was localized in the myoplasm in eggs of four urodele ascidian species that develop into swimming tadpole larvae, this protein was distributed uniformly in eggs of three anural ascidian species. The eggs of two of these anural species contained the actin lamina, another component of the myoplasm, whereas the third anural species lacked the actin lamina. There was no detectible localization of p58 after fertilization or segregation into muscle lineage cells during cleavage of anural eggs. NN18 antigen was uniformly distributed in pre-vitellogenic oocytes and then localized in the perinuclear zone during vitellogenesis of urodele and anural ascidians. Subsequently, NN18 antigen was concentrated in the peripheral cytoplasm of post-vitellogenic oocytes and mature eggs of urodele, but not anural, ascidians. It is concluded that the myoplasm of ascidian eggs contains an intermediate filament-like cytoskeletal network which is missing in anural species that have modified or eliminated the tadpole larva.
Comparison of voice F0 responses to pitch-shift onset and offset conditions.

In order to maintain a steady voice fundamental frequency (F0), it is assumed that people compare their auditory feedback pitch with an internal (memory) or external (acoustic) referent. In the present study we examined whether the internal referent is fixed or variable by comparing voice F0 responses to incorrect auditory feedback in two timing conditions. In one condition, the incorrect pitch was introduced during vocalization (ON condition). In the second, the incorrect auditory feedback pitch was presented before vocal onset and then removed during vocalization (OFF condition). These conditions were examined with pitch-shift stimuli of ±25, 100, and 200 cents. There were no differences in response latency or magnitude between the two timing conditions, indicating that for a sustained-pitch vocalization task, the internal referent is not fixed. Several alternative types of referencing are discussed, which include use of a pitch relative to that which existed at the onset of vocalization (a sample and h.
Marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough.

A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20T) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaCl concentration range for growth was 0-120 g l(-1), with the optimum at 10-20 g l(-1). The temperature range for growth at pH 7.0 was 4-50 degrees C, with the optimum at 37-40 degrees C. The optimum pH for growth was 7.0-8.0. The optimum pressure for growth was 0.1 MPa with tolerance up to 30 MPa. The main cellular phospholipids were phosphatidylglycerols (25 %), diphosphatidylglycerols (34 %) and a group of compounds tentatively identified as ammonium-containing phosphatidylserines (32 %); phosphatidylethanolamines (9 %) were minor components. The fatty acid composition was dominated by side chains of 16 : 0, 14 : 0 and 16 : 1. The G+C content of the genomic DNA was 42 mol%. On the basis of 16S rRNA gene sequence analysis and the secondary structure of the V6 region, this organism was found to belong to the genus Marinilactibacillus and was closely related to Marinilactibacillus psychrotolerans M13-2(T) (99 %), Marinilactibacillus sp. strain MJYP.25. 24 (99 %) and Alkalibacterium olivapovliticus strain ww2-SN4C (97 %). Despite the high similarity between their 16S rRNA gene sequences (99 %), the DNA-DNA hybridization levels were less than 20 %. On the basis of physiological and genetic characteristics, it is proposed that this organism be classified as a novel species, Marinilactibacillus piezotolerans sp. nov. The type strain is LT20T (=DSM 16108T=JCM 12337T).
Pattern Analysis of Lymph Node Metastasis and the Prognostic Importance of Number of Metastatic Nodes in Ampullary Adenocarcinoma

The aims of this study were to clarify the distribution and spread pattern of metastatic nodes and to evaluate the importance of the number, ratio, and location of positive nodes in ampullary adenocarcinoma. We analyzed the clinicopathologic data and survival of 52 patients who received curative pancreatoduodenectomy for ampullary adenocarcinoma between June 1994 and May 2009. Metastatic lymph nodes were found in 32 (61.5%) patients. The median number of evaluated nodes and positive nodes were 26 (range 10-60) and two (range 1-15), respectively. The most commonly involved nodes were the posterior pancreaticoduodenal nodes (26 patients) followed by the anterior pancreaticoduodenal nodes (11 patients). No positive hepatoduodenal and common hepatic artery nodes were found. In univariate analysis, number of positive nodes, and their ratio and location were significantly associated with survival. Only the factor of three or more metastatic nodes had the independent power in predicting a poor outcome in multivariate analysis (P < 0.001). Ampullary adenocarcinoma first spreads to the posterior pancreaticoduodenal nodes and then the anterior nodes. The number of positive lymph nodes, rather than their ratio and location, independently affects survival after curative resection in patients with ampullary carcinoma.
5-HT2C receptor activation by m-chlorophenylpiperazine detected in humans with fMRI

Functional brain imaging using selective drug probes offers the opportunity to investigate regional neuronal activation linked to receptor stimulation or inhibition. In a placebo-controlled, balanced order design in eight male volunteers, the 5-HT2C agonist, m-chlorophenylpiperazine (mCPP) increased blood oxygen level dependent (BOLD) signal in the hypothalamus, caudate, pallidum, amygdala and pyriform cortex, anterior cingulate gyrus and choroid plexus (p < 0.001 uncorrected), areas with high density of 5-HT2C receptors. Activation in the hypothalamus correlated significantly with the prolactin response (p < 0.05 small volume corrected). In a subsequent Go/NoGo task, mCPP enhanced activation in right lateral orbitofrontal cortex (p < 0.05 small volume corrected). These findings suggest that pharmacoMRI is a potentially powerful tool for investigating neurotransmitter function in humans.
Implemented myeloma management with whole-body low-dose CT scan: a real life experience

Abstract A total of 318 consecutive myeloma patients underwent whole-body low-dose CT scan (WBLDCT) at baseline and during follow-up as a radiological assessment of lytic lesions in place of skeletal X-ray survey. After WBLDCT baseline assessment, 60% had bone involvement. The presence of lytic lesions represented the only met CRAB (hyperCalcaemia, Renal insufficiency, Anaemia, Bone lesions) criteria in 29% of patients. Patients presenting with extramedullary masses were 10%. Radiological progression was documented in 9% of the population with available follow-up. Additional pathological incidental findings were detected in 28 patients (14.5%), most located in the chest region (68%). In conclusion, our real-life data shows that WBLDCT scan represents a reliable imaging tool for decision-making process for multiple myeloma management in different disease phases, providing significant additional information on the presence of soft tissues plasmacytomas detection as well as the presence of pathological incidental findings.