Miracle-dz/UniFAR-FTUs huggingface dataset:
Charge transfer through DNA nanoscaled assembly programmable with DNA building blocks

DNA nanostructures based on programmable DNA molecular recognition have been developed, but the nanoelectronics of using DNA is still challenging. A more rapid charge-transfer (CT) process through the DNA nanoassembly is required for further development of programmable DNA nanoelectronics. In this article, we present direct absorption measurements of the long-range CT over a 140-Å DNA assembly based on a GC repetitive sequence constructed by simply mixing DNA building blocks. We show that a CT through DNA nanoscale assembly is possible and programmable with the designed DNA sequence.
Provision of Micronutrient-Fortified Food From 6 Months of Age Does Not Permit HIV-Exposed Uninfected Zambian Children to Catch Up in Growth to HIV-Unexposed Children: A Randomized Controlled Trial

Background:HIV-exposed, uninfected (HIV-EU) children represent a large proportion of children in southern Africa. The reasons for their poorer growth and higher morbidity and mortality than their HIV-unexposed peers are unclear. Objective:We compared anthropometry of 125 HIV-EU with 382 HIV-unexposed young Zambian children participating in a trial of micronutrient-fortified complementary/replacement food. Design:The randomized controlled trial provided children from age 6 to 18 months with a porridge flour containing either a basal or a rich level of micronutrients. Weight and length were measured 3 monthly and head and arm circumferences and triceps and subscapular skinfolds 6 monthly. Results:There were no significant anthropometric differences between the 2 treatment groups. In unadjusted analyses, most anthropometric Z scores of HIV-EU children were lower than those of HIV-unexposed children; after adjustment for treatment arm, socioeconomic factors, breastfeeding and sex, head and arm circumference Z scores remained lower. Subscapular skinfold Z scores were lower among HIV-EU than HIV-unexposed children at 6 months but not 18 months. Conclusions:Socioeconomic factors accounted for some but not all of the impaired growth of HIV-EU children. Micronutrient malnutrition may not be the socioeconomic factor responsible for the growth faltering. Factors acting earlier in life had irreversible effects.
The basal flux of Akt in the mitochondria is mediated by heat shock protein 90

Akt is a known client protein of heat shock protein 90 (HSP90). We have found that HSP90 is responsible for Akt accumulation in the mitochondria in unstimulated cells. Treatment of SH‐SY5Y neuroblastoma cells and human embryonic kidney cells with the HSP90 inhibitors novobiocin and geldanamycin caused substantial decreases in the level of Akt in the mitochondria without affecting the level of Akt in the cytosol. Moreover, intracerebroventricular injection of novobiocin into mice brains decreased Akt levels in cortical mitochondria. Knockdown of HSP90 expression with short interfering RNA also caused a significant decrease in Akt levels in the mitochondria without affecting total Akt levels. Using a mitochondrial import assay it was found that Akt is transported into the mitochondria. Furthermore, it was found that the mitochondrial import of Akt was independent of Akt activation as both an unmodified Akt and constitutively active mutant Akt; both readily accumulated in the mitochondria in an HSP90‐dependent manner. Interestingly, incubation of isolated mitochondria with constitutively active Akt caused visible alterations in mitochondrial morphology, including pronounced remodeling of the mitochondrial matrix. This effect was blocked when Akt was mostly excluded from the mitochondria with novobiocin treatment. These results indicate that the level of Akt in the mitochondria is dependent on HSP90 chaperoning activity and that Akt import can cause dynamic changes in mitochondrial configuration.
Effects of rapid tryptophan depletion on salivary and plasma cortisol in Alzheimer's disease and the healthy elderly

Serotonergic function is reduced in dementia of Alzheimer type (DAT) and abnormalities in the hypothalamic-pituitary-adrenal (HPA) axis are also common. There is considerable interaction between the two systems. Effects of lowering brain serotonin on salivary and plasma cortisol were assessed in patients with DAT and in control subjects. A double-blind, cross-over design involving administration of two nutritionally balanced amino acid mixtures with or without tryptophan was used. Salivary and plasma cortisol were measured at intervals before and after the drink. DAT patients had higher salivary cortisol than controls. Despite a reduction of approximately 70% in plasma free tryptophan after 4 h in both groups, there was no effect on salivary or plasma cortisol. We conclude that, in subjects with DAT and healthy elderly subjects, acute tryptophan depletion had no effect on cortisol secretion.
The isolation of strict anaerobes: the use of an anaerobic cabinet compared with a conventional procedure.

Summary An account is given of the design and development of an anaerobic cabinet for the manipulation of anaerobic cultures. Results of comparative studies on the recovery of anaerobic bacteria show that the quantitative recoveries obtainedb from cultures of strict anaerobes such as Clostridium oedematiens (C. novyi8 type D and C. tetani were not improved by the use of the cabinet. Total count of anaerobes from faecal samples, whether manipulated in the cabinet or at the bench by a careful conventional procedure, were almost identical. It is sub-mitted that the quantitative growth of many anaerobic human pathogens does not require the use of ultra-specialised equipment. We thank Dr B. S. Drasar for helpful advice and the Agricultural Research Council for financial support (Grant AG 15/57) that enabled us to build the anaerobic cabinet. The Medical Research Council (Grant G 971 /113/B) generously supported the investigations that we report here.
Critical care management of cerebral venous thrombosis

Purpose of reviewAlthough recent trials of intervention for acute ischemic stroke have been positive, similar benefit in acute cerebral venous thrombosis (CVT) remains largely unclear. This review aims to summarize the existing evidence regarding the management of CVT, including anticoagulation and endovascular therapy. Recent findingsThe mainstay of treatment in CVT is systemic anticoagulation even in the setting of intracerebral hemorrhage. Nonrandomized studies and case series suggest that endovascular therapy in CVT is relatively safe, and can improve outcomes in the small subset of CVT patients with neurologic deterioration despite anticoagulation. SummaryDespite a generally favorable prognosis, one in four patients with CVT develop neurological deterioration in the acute phase. Predisposing factors include a neurological deficit or seizures at onset, deep venous thrombosis, venous infarctions, or intracranial hemorrhage with mass effect and an underlying thrombophilia. More randomized trials are needed to compare the benefits of anticoagulation and endovascular therapy.
Effects of a 6-month incentive-based exercise program on adherence and work capacity.

The purpose of the study was to evaluate the effect of behavioral management techniques on exercise adherence linked to improvements in work capacity and maximal oxygen consumption (VO2max). One hundred thirty-seven participants in six different worksites on a university campus (five experimental and one comparison site) completed 6 months of a minimally supervised, incentive-based endurance exercise program. All participants in the experimental group contracted to engage in at least four bouts of 30 min of verified aerobic exercise within a prescribed target heart rate range each week for the duration of the program. Forty dollars deposited at the beginning of the program served as a response cost that could be lost as a result of failure to fulfill the weekly contracts. Individuals in the comparison group participated in a similar 6-month program but without the contracts and response cost strategies. Weekly adherence for both groups was strictly defined as verified fulfillment of all four bouts of exercise. Adherence for the experimental group was 97% by this definition, and adherence for the comparison group was 19% (P less than 0.01). VO2max increased 2.6% (P less than 0.01), and treadmill test time increased 16% (P less than 0.01) in the experimental group after the 6-month program, with no significant changes in the comparison group. Recovery heart rates at 2 and 4 min post-exercise were significantly lower at 6 months in the experimental group but not in the comparison group. These data provide evidence that adherence to a 6-month endurance exercise program can be improved significantly through the use of well conceived behavior management strategies. (ABSTRACT TRUNCATED AT 250 WORDS)
Management of salivary gland adenoid cystic carcinoma: institutional experience of a case series.

CONTEXT AND OBJECTIVE Salivary gland tumor management requires long-term follow-up because of tumor indolence and possible late recurrence and distant metastasis. Adenoid cystic carcinoma (ACC) accounts for 10-15% of such tumors. The aim here was to evaluate surgical and clinical management, staging and follow-up of ACC patients in one academic institution. DESIGN AND SETTING Retrospective study at Head and Neck Service, Universidade Estadual de Campinas. METHODS Data on 21 patients treated between 1993 and 2003 were reviewed. Management utilized clinical staging, histology and imaging. Major salivary gland tumor extent was routinely assessed by preoperative ultrasonography. Diagnosis, surgery type, margin type (negative/positive), postoperative radiotherapy and recurrence (presence/absence) were evaluated. RESULTS There were eleven major salivary gland tumors (52.3%), seven submandibular and four parotid. Ten patients (47.7%) had minor salivary gland ACC (all in palate), while the submandibular was the most frequently affected major one. Diagnoses were mostly via fine-needle aspiration (FNA) and incision biopsy. Frozen sections were used for six patients. There was good ultrasound/FNA correlation. Sixteen (76%) had postoperative radiotherapy. One (4.7%) died from ACC and five now have recurrent disease: three (14.2%) locoregional and two (9.5%) distant metastases. CONCLUSION Adenoid cystic carcinoma has locally aggressive behavior. In 21 cases, of ACC, the facial nerve was preserved in all except in the few with gross tumor involvement. Treatment was defined from physical examination, imaging, staging and histology.
Cloning and Functional Expression of a New Water Channel Abundantly Expressed in the Testis Permeable to Water, Glycerol, and Urea*

A new member of the aquaporin (AQP) family has been identified from rat testis. This gene, referred as aquaporin 7 (AQP7), encodes a 269-amino acid protein that contained the conserved NPA motifs of MIP family proteins. AQP7 has the amino acid sequence homology with other aquaporins (∼30%), and it is highest with AQP3 (48%), suggesting that both AQP3 and AQP7 belong to a subfamily in the MIP family. Injection of AQP7-cRNA into Xenopus oocytes expressed a 26-kDa protein detected by immunoblotting. The expression of AQP7 in oocytes stimulated the osmotic water permeability by 10-fold which was not inhibited by 0.3 mm mercury chloride. The Arrhenius activation energy for the stimulated water permeability was low (2.1 kcal/mol). AQP7 also facilitated glycerol and urea transport by 5- and 9-fold, respectively. The activation energy for glycerol was also low (5.3 kcal/mol after the correction of the endogenous glycerol permeability of oocytes). Northern blot analysis revealed a 1.5-kilobase pair transcript expressed abundantly in testis. In situ hybridization of testis revealed the expression of AQP7 at late spermatids in seminiferous tubules. The immunohistochemistry of testis localized the AQP7 expression at late spermatids and at maturing sperms. AQP7 may play an important role in sperm function.
Molecular Determinants and Role of An Anion Binding Site in the External Mouth of the CFTR Chloride Channel Pore

Chloride permeation through the cystic fibrosis transmembrane conductance regulator (CFTR) Cl− channel is blocked by highly lyotropic permeant anions which bind tightly within the pore. Here we show that several different substitutions of a positively charged amino acid residue, arginine R334, in the putative outer mouth of the CFTR pore, greatly reduce the block caused by lyotropic Au(CN)2− ions applied to the intracellular side of the channel. Fixed positive charge at this site appears to play a role in Au(CN)2− binding, as judged by multiple substitutions of differently charged amino acid side chains and also by the pH dependence of block conferred by the R334H mutant. However, non‐charge‐dependent effects also appear to contribute to Au(CN)2− binding. Mutation of R334 also disrupts the apparent electrostatic interaction between intracellular Au(CN)2− ions and extracellular permeant anions, an interaction which normally acts to relieve channel block. All six mutations studied at R334 significantly weakened this interaction, suggesting that arginine possesses a unique ability to coordinate ion‐ion interactions at this site in the pore. Our results suggest that lyotropic anions bind tightly to a site in the outer mouth of the CFTR pore that involves interaction with a fixed positive charge. Binding to this site is also involved in coordination of multiple permeant anions within the pore, suggesting that anion binding in the outer mouth of the pore is an important aspect in the normal anion permeation mechanism.
Green Premium Evidence from Climatic Areas: A Case in Southern Europe, Alicante (Spain)

The existence of a green premium in house (asking) prices in Alicante province, Spain, are analyzed using circa 9000 property observations. In developing the sample, information from energy efficiency certificates was matched with two other databases. The model tests for green premium by climatic zones using pool Ordinary Least Squares (pool-OLS) and Instrumental Variables (IV) hedonic models, adds new knowledge concerning the existence of green premiums from Southern Europe, explores differences in their estimation by climatic zone, debates the nature of the estimated green parameters, and explains the role of endogeneity in hedonic green premium models. The empirical evidence assesses the sensitivity of asking price to either energy consumption (KWh) or carbon dioxide emissions (CO2) with an apparent premium of 3%, and captures an association with efficiency rating from G to F of 1.8% and from F to E of 1.1%. Significantly, the results relating to price responses show a distinct variation between the coast and the cooler climatic zone of the interior. The paper shows that energy efficiency incentive policies should discriminate by climatic areas, and provides a price reference by which to assess the amount of incentives needed to achieve European Union (EU) objectives.
Dosimetric consequences of a translational isocenter correction based on image guidance for intensity modulated radiotherapy (IMRT) of the prostate

Interfractional prostate motion during radiotherapy can have deleterious clinical consequences. It has become clinical practice to re-position the patient according to ultrasound or other imaging techniques. We investigated the dosimetric consequences of the linear translational position correction (isocenter correction) when a conformal IMRT technique with nine fields was used. Treatment plans of seven patients with empty and distended rectums were analyzed. The reference plans were calculated on the CT with an empty rectum. The treatment plans were transferred to a second CT with a distended rectum for an uncorrected setup of the patient referenced to bony anatomy and a corrected setup after translational position correction of the isocenter. The dosimetric consequences (with and without correction) were analyzed. For single treatment fractions, organ motion decreased the volume of the prostate encompassed by the 95% isodose (V95%) by up to −24%-p (percentage points). The mean rectum dose increased by up to 41%-p. Linear translational correction increased V95% of the prostate by up to 17%-p while the mean rectum dose was reduced by up to −23%-p compared to the uncorrected setup. Linear translational correction can improve radiation treatment accuracy for prostate cancer if geometrical changes are within certain limits.
Sensory system function and postural stability in men aged 30–80 years

Objective. To examine age-related changes in postural stability and sensory system functioning in men aged 30–80 years. Design. Observational, cross-sectional study. Methods. One hundred six healthy men aged 30–80 years participated. Personal characteristics were recorded and outcome measures included: velocity of sway during bilateral stance on a firm and foam surface (eyes open (EO) and eyes closed (EC)), balancing on one leg (EO and EC), lower limb somatosensation (tactile acuity, vibration threshold and joint position error), high-contrast visual acuity (HCVA) and low-contrast visual acuity (LCVA), edge contrast sensitivity and vestibular-ocular reflex (VOR) control. Results. Men in their 60s and 70s were found to be less stable than the younger age decades when standing on a firm or foam surface. Reduced stability was evident from the 40s to 50s for one-leg-stance (EC). Lower limb somatosensation and HCVA and LCVA were significantly reduced by the 60s but edge contrast sensitivity reduced by the 50s. Age-related changes in VOR control did not emerge until the 70s in this study cohort. Conclusions. The results show reduction in postural stability and sensory system functioning in men by the 60s supporting pre-emptive assessment of workers in industries where falls are frequent.
Necessarily or Sufficiently Trade-Impeding for Africa?1 The Agri-food Safety Regulations

Abstract The dearth of empirical studies on Africa’s food export effects of the food safety regulations in the European Union has really affected evidence-based policies that could stimulate compliance. This study investigates whether the technical regulations necessarily or sufficiently constitute trade-impediment for Africa. This study adopts an augmented Helpman, Melitz and Rubenstein two-step selection model for all the applicable food safety regulations in this market. Three commodities are selected; Banana, Grape and Tomato at the HS-6-digit level covering the period from 1995 to 2016. Owing to the heterogeneity in exporters’ capacity and exporting performance as well as their size structure, this study finds that the food safety regulations are necessarily trade-impeding for the grapes and tomatoes but not for banana at the decision to export stage. However, at the intensity of the commodities’ exporting, the measures become sufficiently trade-enhancing due to quality standards upgrading, compliance and certification of exports.
A Comparative Policy Analysis of Elementary Teacher Education in the Top Performing Asian TIMSS Countries

ABSTRACT: This paper analyzed elementary TED (Teacher Education) programs in the top performing TIMSS ( Trends in International Mathematics and Science Study ) Asian, i.e. Japan, South Korea, and Singapore countries to explore how much emphasis should be placed on general content knowledge, versus general pedagogical knowledge, and versus methodological pedagogical knowledge. Three theoretical frames of reference were analyzed during this process: whether the programs were consecutive or concurrent; the model of partnership followed between universities and field experience institutions; and the overall status of teachers in the society as categorized by position-based or career-based. Methodologically, only peer-reviewed literature published between 2000 and 2018 was used. It was found that the top performing Asian TIMSS countries usually: have consecutive and concurrent options; provide very intensive TED experiences to their students focusing on diverse and practical field experiences; place more emphasis on academic subject expertise than pedagogy; require students to major in at least one academic subject; have national accreditation institutes for unifying standards; their sponsor countries enforce various types of induction and professional development once in the field; offer salaries competitive with other professions that require the same amount of years and training; and, most importantly, only accept the top academic achievers into their programs. One of the recommendations is that since Saudi Arabia has just initiated “Vision 2030”, a sort of privatization schema for the entire economy, it seems higher selection criteria will be critical to TED in line with this national vision. KEY WORDS: Teacher Education; Trends in International Mathematics and Science Study ; Asian Countries; Comparative Study. About the Author: Dr. Mohammed Sabrin is an Assistant Professor at the College of Education, Taibah University, Office SB-29, College of Education Building B-13, Al-Madinah al-Munawwarah, Kingdom of Saudi Arabia. E-mail: msabrin@taibahu.edu. sa Suggested Citation: Sabrin, Mohammed. (2018). “A Comparative Policy Analysis of Elementary Teacher Education in the Top Performing Asian TIMSS Countries” in EDUCARE: International Journal for Educational Studies , Volume 11(1), August, pp.1-16. Bandung, Indonesia: Minda Masagi Press owned by ASPENSI with ISSN 1979-7877 (print) and ISSN 2621-587X (online). A rticle Timeline : Accepted (February 17, 2018); Revised (May 2, 2018); and Published (August 30, 2018).
Granulocyte colony‐stimulating factor dosing in pegylated interferon alpha‐induced neutropenia and its impact on outcome of anti‐HCV therapy *

Summary. Many patients with chronic hepatitis C (HCV) infection undergoing treatment with pegylated interferon‐alpha (PEG‐IFN‐alpha) and ribavirin develop neutropenia requiring dose reduction or granulocyte colony‐stimulating factor (G‐CSF) supplement. We analysed the database of patients who completed treatment for chronic HCV infection between 2003 and 2006. Patients with absolute neutrophil counts below 1000 cells/μL were initiated on G‐CSF (G‐CSF group) while a matching group of patients who received anti‐HCV treatment without developing neutropenia were used as a control group (non‐G‐CSF group). Patients on the G‐CSF arm were divided into two subgroups based on the timing of G‐CSF administration relative to PEG‐IFN‐alpha administration. Of the 163 patients with HCV infection, 30 patients received G‐CSF, most of who were maintained on 300 μg of G‐CSF once a week. Administration of G‐CSF 2 days before or after each dose of PEG‐IFN‐alpha did not make a significant difference in the neutrophil counts. In the G‐CSF arm, 23 of 30 patients (77%) had undetectable end‐of‐treatment viral response which was comparable with 27 of 30 in the control group (90%; P = 0.17). There was no statistically significant difference in the sustained viral response between the two groups (61%vs 76%, P = 0.18). In most patients PEG‐IFN‐alpha induced neutropenia improved with a once‐a‐week dose of G‐CSF with a comparable virological outcome. Timing of G‐CSF administration did not make any significant impact on the patient's neutrophil counts but was better tolerated when given 2 days apart from PEG‐IFN‐alpha.