Miracle-dz/UniFAR-FTUs huggingface dataset:
Structural determinant of the species-specific transcription of the mouse rRNA gene promoter

Mammalian ribosomal DNA (rDNA) transcription has a certain species specificity such that, both in vivo and in vitro, human rDNA cannot be transcribed by mouse machinery and vice versa. This is due to a species-dependent transcription factor, TFID (Y. Mishima, I. Financsek, R. Kominami, and M. Muramatsu, Nucleic Acids Res. 10:6659-6670, 1982). On the basis of the information obtained from 5' and 3' substitution mutants, we prepared a chimeric gene in which the mouse sequence from positions -32 to -14 was inserted into the corresponding location of the human rDNA promoter. The chimeric gene could be transcribed by mouse extracts nearly as efficiently as the wild-type mouse promoter. The chimeric gene could also sequester transcription factor TFID at an efficiency similar to that for the mouse promoter. Partially purified mouse TFID that could not protect the human rDNA promoter against DNase I produced a clear footprint on this chimeric gene that was similar to that on mouse rDNA promoter. The basic structure of the mouse rDNA core promoter is discussed in relation to the interaction with TFID.
Correlations between dynamic urethral profilometry and perivaginal pelvic muscle activity

Of 208 ambulatory female subjects evaluated for complaints of urinary incontinence, complete history, physical findings, and urodynamic dala were available on 163 patients allowing correlation of measures of perivaginal muscle activity to urcthral profilometry measurement of sphincter strength. Perivaginal measures included pelvic digital exam score as well as vaginal electromyography with a modified perinometer. Urethral profilometry was performed at rest and during pelvic muscle contractions in both supine and standing positions. There was a moderate and significant correlation (r = 0.19 to 0.32) between profilometry measures of voluntary sphincter contractions and perivaginal EMG parameters of endurance peak and area, as well as to the digital lest parameters of pressure and displacement. The correlation values between the vaginal EMG and the Digital Test scale of perivaginal strength were higher (r = 0.28 to 0.74). When the patients with pure stress incontinence were stratified by degrees of incontinence (mild, moderate, severe), urethral profilometry measures were a more accurate indicator of severity of incontinence than measures of perivaginal strength or the degree of bladder neck mobility as measured by the Q‐tip test. © 1994 Wiley‐Liss, Inc.
Evaluation of Uridine Metabolism in Human and Animal Spermatozoa

The objective of this study was to elucidate the role of uridine for spermatozoa, since this pyrimidine nucleoside was found in millimolar concentration in human seminal plasma. Here, the degradative activity of uridine-phosphorylase [EC 2.4. 2.3] and the salvage activity of uridine kinase [EC 2.7. 1.48] were detected in human spermatozoa. HPLC analysis depicted the uptake of exogeneous 14C-labelled adenine, but not of uridine and of hypoxanthine, into nucleotide pools of boar spermatozoa. On addition of uridine, the computer-assisted semen analysis (CASA) of human cells revealed a reduction of the percentage of motile spermatozoa in contrast to an elevation of some velocity parameters. It is concluded that exogeneous uridine could function as suppressor for early capacitation and as a substrate for phosphorolysis, if ribose is needed, rather than to satisfy a demand for intracellular pyrimidine nucleotides.
Effect of ablation of medullary emetic chemoreceptor trigger zone on vomiting responses to cerebral intraventricular injection of adrenaline, apomorphine and pilocarpine in the cat

Vomiting is a frequent response to drugs injected into the cerebral ventricles in cat, dog and man (Cushing, 1931; Feldberg & Sherwood, 1954; Weinberg & Haley, 1955). Ablation of the emetic chemoreceptor trigger zone in the medulla oblongata abolishes vomiting in response to certain drugs given by systemic routes of administration in dog and cat (Borison & Wang, 1953). The experiments reported here show that in cats ablation of the chemoreceptor trigger zone abolishes the emetic response to adrenaline and apomorphine injected into the cerebral ventricles and reduces the incidence of vomiting in response to intraventricular pilocarpine. A preliminary account of the results has been given to the Physiological Society (Borison, 1958).
Clopidogrel bioavailability: absence of influence of food or antacids.

Two open, randomized, crossover bioavailability studies were carried out to assess the influence of concurrent antacid medication and food on the bioavailability of clopidogrel. A fed/fasting study was conducted in 12 elderly male subjects. Each subject took a single 75 mg dose of clopidogrel on two occasions-in the morning after an overnight fast, either during a standardized breakfast, or with breakfast delayed by 4 hours after dosing. A washout period of 7 days was observed between the two dosings. Twelve healthy male subjects participated in the antacid study. They fasted overnight and for 4 hours after dosing and took a single 75 mg dose of clopidogrel at 8:00 a.m. on two occasions separated by a washout period of 14 days. For one dose, Maalox 2 x 400 mg tablets were taken 1 hour before the clopidogrel dose. Pharmacokinetic parameters of SR26334, the main circulating metabolite of clopidogrel, were derived from plasma concentrations of the latter compound determined before and at regular intervals over 36 hours after dosing. For the fed/fasting study, mean Cmax values (+/-SD) were 2.7+/-0.62 mg/L and 2.1+/-0.96 mg/L for the fasting state and the fed state, respectively and the 90% CI of Cmax ratio was [0.57 - 0.97]. Mean AUC(0-obs) values (AUC to the last observed value) were 7.1+/-1.6 mg.h/L and 7.4+/-1.64 mg.h/L, respectively, and the 90% Cl of AUC ratios were [0.90 - 1.02] and [0.89 - 0.97], respectively. For the antacid study, mean Cmax values were 2.6+/-0.84 mg/L and 2.5+/-0.87 mg/L for the no-antacid regimen and the antacid regimen, respectively, and the 90% CI of Cmax ratio was [0.74 - 1.16]. Mean AUC(0-obs) values were 6.3+/-1.34 mg.h/L and 5.8+/-1.33 mg.h/L, respectively, and the 90% CI of AUC ratios was [0.89+/-0.97]. Thus, exposure to SR26334, and therefore net absorption of clopidogrel, was not significantly modified by food or by prior antacid ingestion.
An efficient method for prefilter computation for reduced-state equalization

In advanced TDMA mobile communications systems, reduced-state equalization algorithms have to be employed because a high-level modulation is used in order to improve the spectral efficiency. Such equalizers only have a high performance, if the overall discrete-time system to be equalized is minimum-phase. Therefore, in general, a discrete-time prefilter has to be inserted in front of equalization. In the literature, several approaches have been proposed for computation of a suitable FIR or IIR prefilter. We present an approach for FIR prefilter computation, which is quite robust and requires an only moderate computational complexity. The prefilter consists of the cascade of a channel-matched filter and a prediction-error filter, which can be calculated via the Levinson-Durbin algorithm. Simulation results are given, which demonstrate that the performance of the proposed approach is essentially equivalent to the case of reduced-state equalization combined with ideal allpass prefiltering.
Daytime sleepiness and changes of sleep architecture in patients with epilepsy.

OBJECTIVE The relationship between epilepsy and sleep has been known for many years. Yet is still not well understood because of it's reciprocal and intrinsic influences. Epileptic manifestations during sleep may lead to fragmentation of sleep stages. On the other hand insomnia or other sleep disorders may cause sleep deprivation and increase number of epileptic seizures in patients with epilepsy. The study is designed to compare daytime sleepiness and architecture of sleep in patients with epilepsy and control subjects. We tried to evaluate factors that have influence on sleep architecture of patients with epilepsy. METHODS We evaluated the daytime sleepiness through a certain type of questionnaire called Epworth Scale of Sleepiness (ESS). The questionnaire was filled out by patients with epilepsy (83 patients) and a group of healthy controls (80 persons). Furthermore we evaluated the quatity of night sleep in both groups using polysomnograph (electroencephalography - EEG, electrooculography - EOG, electromyography - EMG). Sleep stages were scored according to Manual of Standardized Terminology, Techniques and Scoring System for Sleep Stages of Human Subjects (Rechtschaffen, Kales, 9). Multiple sleep latency test (MSLT) was performed in the group of patients with epilepsy in order to objectivise daytime sleepiness. RESULTS Measuring by Epworth scale of sleepiness we found out that the patients with epilepsy suffered from a significantly higher daytime sleepiness than the healthy controls. Patients with epilepsy also experienced significant changes in sleep architecture, the reduction of NREM S3, S4 and REM stages and the increase of NREM S2 stage. These changes were not related to used antiepileptic medication.
Cholinergic activation and tonic excitation induce persistent gamma oscillations in mouse somatosensory cortex in vitro

1 Concomitant application of the cholinergic agonist carbachol and nanomolar doses of kainate can elicit persistent gamma frequency oscillations in all layers of the mouse somatosensory cortex in vitro. Receptor pharmacology with bath‐applied antagonists indicated that oscillatory network activity depended crucially on the participation of cholinergic muscarinic, (S)‐α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA)/kainate and GABAA receptors. 2 The timing of action potentials and the occurrence of excitatory as well as inhibitory postsynaptic events was highly correlated with the phasic change of extracellularly recorded population activity. Firing probability was lowest during the peak negativity of IPSPs and gradually increased during their ensuing decay. In conjunction with the effect of a barbiturate to decrease the frequency of gamma oscillations, this suggests a crucial role of IPSPs in phasing the suprathreshold activity of principal neurons. 3 At nearby (< 1 mm) sites contained within any given cortical layer, oscillatory extra‐ and intracellular activity was highly synchronous with no apparent phase lag. However, interlaminar mapping experiments demonstrated a phase reversal of both extra‐ and intracellularly recorded activity near the lower border of thalamo‐recipient layer 4, thus corroborating findings that have been obtained in vivo. 4 In conclusion, a modest increase of tonic excitatory drive in conjunction with the activation of cholinergic muscarinic receptors can elicit persistent gamma frequency network oscillations in the rodent somatosensory cortex. These findings (re)emphasize the role of the cholinergic ascending system in the cortical processing of sensory information.
Distributed Virtual Network Embedding with Bloom Filter

As a disruptive technology, Network Virtualization plays an increasingly important role in Cloud Computing by allowing multiple heterogeneous virtual networks to run on a shared infrastructure as independent slices. However, it is a big challenge to map multiple virtual networks onto specific nodes and links in the shared substrate network, known as the Virtual Network Embedding (VNE) problem. Most existing solutions are provided in a centralized way, which may have hot spot problem. In this paper, by leveraging the learning and inference technology, a novel VNE algorithm is proposed to achieve the embedding in a distributed way. Instead of flooding, Bloom Filter is introduced to synchronize the mapping information, without generating massive communication overhead. The results of comparison between existing algorithms and the proposed algorithm show that our distributed algorithm has acceptable, even better performance in terms of long-term average revenue and acceptance ratio.
28GHz millimeter wave propagation models based on ray-tracing in urban scenario

The millimeter wave spectrum is widely studied as one of the most important resources for the IMT (International Mobile Telecommunication) system towards to the 5G for the large bandwidth. In this paper, its investigated the propagation characteristics of 28GHz millimeter wave using Ray-Tracing in Chengdu city of China. Both the close-in-reference and floating intercept path loss fitting methods combining with the shadow fading are studied. Based on the Ray-Tracing, different path loss fitting method combining different antenna pattern and outage threshold are studied. According to observation of the comparison with these two fitting path loss methods, it is found that close-in-reference method can get more robust attenuation exponent. In addition, its shadow fading deviation will be less than floating intercept method in shorter transmit distance, and only increase less than 2dB for longer transmit distance. A certain amount of outage receiver point are found and the influence to path loss are analyzed. For the high direction antenna pattern, if it can not capture the strong path, it will also influence the path loss results.
Changes in the population of larger cities of the USSR, 1979-1989.

"An overview of the dynamics of Soviet cities of over 100,000 population for the period 1979-1989 is presented, based largely on maps and tables depicting five key 'subsets' or city groupings: (a) cities increasing by over 100,000 inhabitants; (b) the fastest growing cities in percentage terms; (c) their comparison with fastest growing cities, 1959-1979; (d) the slowest growing cities in percentage terms; and (e) their comparison with slowest growing cities, 1959-1979. The paper, by focusing on these parameters and utilizing extensively graphic and cartographic methods of data presentation, provides.insights into city growth trends. "
A Sleeping Beauty transposon-mediated screen identifies murine susceptibility genes for adenomatous polyposis coli (Apc)-dependent intestinal tumorigenesis

It is proposed that a progressive series of mutations and epigenetic events leads to human colorectal cancer (CRC) and metastasis. Furthermore, data from resequencing of the coding regions of human CRC suggests that a relatively large number of mutations occur in individual human CRC, most at low frequency. The functional role of these low-frequency mutations in CRC, and specifically how they may cooperate with high-frequency mutations, is not well understood. One of the most common rate-limiting mutations in human CRC occurs in the adenomatous polyposis coli (APC) gene. To identify mutations that cooperate with mutant APC, we performed a forward genetic screen in mice carrying a mutant allele of Apc (ApcMin) using Sleeping Beauty (SB) transposon-mediated mutagenesis. ApcMin SB-mutagenized mice developed three times as many polyps as mice with the ApcMin allele alone. Analysis of transposon common insertion sites (CIS) identified the Apc locus as a major target of SB-induced mutagenesis, suggesting that SB insertions provide an efficient route to biallelic Apc inactivation. We also identified an additional 32 CIS genes/loci that may represent modifiers of the ApcMin phenotype. Five CIS genes tested for their role in proliferation caused a significant change in cell viability when message levels were reduced in human CRC cells. These findings demonstrate the utility of using transposon mutagenesis to identify low-frequency and cooperating cancer genes; this approach will aid in the development of combinatorial therapies targeting this deadly disease.
No place like home: Testosterone responses to victory depend on game location

Several studies have demonstrated that a variety of factors influence testosterone responses to competitive interactions. This study examined the extent to which game location would influence testosterone responses to human competition. Male amateur ice hockey players (n = 10) provided saliva samples before and after competing against the same opponent on two separate occasions (one game at home and one game away). Although both games resulted in similar victories, the home victory was associated with a significantly larger rise in testosterone concentrations relative to the away victory. The factors responsible for the different testosterone responses are not known, however, it is possible that a rise in status in front of the home crowd is more rewarding to athletes, and thus, a more potent stimulus for the endocrine system. Am. J. Hum. Biol., 2009. © 2009 Wiley‐Liss, Inc.
Electrostatic Tuning to Achieve Higher Stability Microelectromechanical Composite Resonators

Electrostatic tuning of the frequency in micromachined Si-SiO2 composite resonators for temperature compensation is demonstrated and analyzed. Electrostatic tuning exploits the bias voltage dependence of frequency for the compensation. Si-SiO2 composite resonators have intrinsically small frequency variation over temperature, thus being appropriate for electrostatic tuning. We developed a tuning procedure and applied it to a flexural-mode composite resonator. Experimental results show ±2.5-ppm stability over a 90°C-wide temperature range.
T lymphocytes derived from skin lesions of patients with psoriasis vulgaris express a novel cytokine pattern that is distinct from that of T helper type 1 and T helper type 2 cells

In various immunological disorders the pathomechanisms of tissue damage are causally associated with specific patterns of locally produced cytokines. To study the molecular and cellular mechanisms involved in the manifestation of psoriasis vulgaris we have assessed the cytokine mRNA profile expressed in lesional psoriatic skin and in T cell clones (TCC) that were established from skin lesions of patients with psoriasis. As demonstrated by use of the polymerase chain reaction (PCR), psoriasis lesions consistently exhibit transcription of a complex pattern of cytokines. It includes mediators selectively produced by T lymphocytes [interferon (IFN)‐γ, tumor necrosis factor (TNF)‐β, interleukin (IL)‐2, IL‐3 and IL‐5] as well as cytokines secreted by various cell types [transforming growth factor (TGF)‐α/‐β, TNF‐α, IL‐6/‐8 and granulocyte‐macrophage‐colony stimulating factor], while IL‐4 is missing. With the exception of TGF‐α, this cytokine profile was also observed in lesional psoriatic T cell clones yielding supernatants mitogenic for keratinocytes in vitro (MTCC), but not in T cell clones yielding supernatants that inhibited keratinocyte proliferation (STCC).
Projective depth: A geometric invariant for 3D reconstruction from two perspective/orthographic views and for visual recognition

The author addresses the problems of reconstructing 3-D space in a projective framework from two views and of artificially generating novel views of the scene from two given views. It is shown that with the correspondences coming from four non-coplanar points in the scene and the corresponding epipoles, it is possible to define and reconstruct a projective invariant, referred to as projective depth, that can be used later to reconstruct the projective or affine structure of the scene or directly to generate novel views of the scene. The derivation has the advantage that the viewing transformation matrix need not be recovered in the course of computations. <<ETX>>
On the decompression melting structure at volcanic arcs and back‐arc spreading centers

Mantle dynamics can strongly affect melting processes beneath spreading centers and volcanic arcs. A 2‐D numerical model of the Tonga subduction zone, with the slab viscously coupled to the mantle beneath the brittle‐ductile transition but faulted above, shows that induced corner flow may cause asymmetric melting at the Lau back‐arc spreading center, 400 km away. The down‐going slab also entrains the high‐viscosity base of the overlying lithosphere, drawing hot, low‐viscosity asthenosphere upwards into the gap, triggering decompression melting in the wedge. Because the slab is decoupled from the brittle overlying plate, a cold upper corner develops, inhibiting melting where the slab is shallow. The cold corner is consistent with seismic attenuation and heat flow at arcs. Decompression melting may be a substantial fraction of magma production at some arcs, but less at others. Possibly more important, the shallow decompression melting structure may govern the pathways of melt extraction beneath volcanic arcs.
Tobacco consumption and its association with illicit drug use among men in Bangladesh.

AIMS This study examined the association of tobacco consumption (smoking and chewing) with illicit drug use among Bangladesh males. DESIGN Cross-sectional survey data from the Bangladesh Demographic and Health Survey 2004 were used. SETTING Bangladesh. PARTICIPANTS A total of 4297 males aged 15-54 years. MEASUREMENTS Age, education, religion, marital status, place of residence; tobacco consumption such as cigarette and bidi smoking, chewing sada, pata, tobacco leaves, gul, betel quid with zarda; taking illicit drugs such as ganja, charas, heroin, pethedine, phensidyl; having sexually transmitted diseases (STDs). FINDINGS Overall prevalence of tobacco consumption was 59%. Bidi smoking (29.6%), cigarette smoking (27.8%) and chewing betel quid with tobacco/zarda (17.5%) were predominant. Overall prevalence of illicit drug use was 4%. Ganja was the main drug (3%), followed by phensidyl (0.8%), heroin (0.3%) and charas (0.3%). Age, education, place of residence, marital status, having STDs, premarital and extra-marital sex were associated significantly with tobacco smoking. Almost all variables were also associated significantly with illicit drug use. Smoking cigarettes and bidi and eating tobacco leaves/shada pata/gul showed significantly positive associations with illicit drug use when adjusted for other variables. CONCLUSIONS Tobacco consumption is common and associated positively with the illicit drug use among males in Bangladesh.
A Clinico-Epidemiological Study of Erythroderma in a Tertiary Care Center in Jharkhand

Erythroderma is an inflammatory skin condition which presents with extensive erythema and scaling of skin. It may be complicated by various metabolic abnormalities and is a life threatening condition. We conducted a prospective study to evaluate the epidemiology, clinical features and etiology of erythroderma in patients attending the dermatology clinic in Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, between December 2015 and April 2017. A total of 34 cases were included in the study. The male to female ratio was 2.4 :1 and the age group most affected was 2130 years. The most common cause of erythroderma was psoriasis(38.23%) followed by drug induced erythroderma(20.59%), atopic dermatitis, pityriasis rubra pilaris, scabies, congenital ichthyosiform erythroderma and others.