Miracle-dz/UniFAR-FTUs huggingface dataset:
MEAT AND FAT CONTENT OF CROSSBRED GILTS BORN AND KEPT IN POLAND IN BYDGOSZCZ BREEDING DISTRICT IN YEARS 1995-2004

The analysis covers findings of performance (tested in vivo) of fat and meat content of 45286 two-breeds crossbreds gilts tested in ten successive years (1995-2004) according to the same methodology observed in those years. Animals were produced in Poland in the area of Bydgoszcz Breeding District, covering Kujawy-Pomorze province. They came from two crossing variants of breeds Polish Large White (PLW) and Polish Landrace (PL): [PLW x PL] and [PL x PLW]; the sow’s breed is given in the first position. Two-factor ANOVA analysis of variance was employed in the research taking into consideration a crossing variant and ten years, i.e. 1995-2004 (referred to as groups 1-10, respectively) when animals’ performance was tested (in vivo). Computer program Statistica PL was used in calculations. Taking into consideration the total results from ten years (1995-2004) it should be noticed that crossbred gilts [PL x PLW] had significantly thinner backfat measured in P2 and P4 points, lower height of loin eye and higher carcass meat content (P≤0.01) compared with animals from [PLW x PL] crossing variant. The analysis of the results from year 1995 and year 2004 shows that in the case of crossbred gilts [PLW x PL] backfat thickness in P2 and P4 points decreased by 6.76 and 7.76 mm and meat content increased by 6.65%. Improvement in the mentioned traits occurred also in the case of gilts from the second tested crossing variant, i.e. [PL x PLW], because backfat thickness in P2 and P4 points decreased by 5.41 and 6.00 mm and carcass meat content increased by 4.80 %.
Metabolic Activation-related CD147-CD98 Complex*S

Cell surface CD147 protein promotes production of matrix metalloproteinases and hyaluronan, associates with monocarboxylate transporters and integrins, and is involved in reproductive, neural, inflammatory, and tumor functions. Here we combined covalent cross-linking, mass spectrometric protein identification, and co-immunoprecipitation to show selective CD147 association with three major types of transporters (CD98 heavy chain (CD98hc)-l-type amino acid transporter, ASCT2, and monocarboxylate transporters) as well as a regulator of cell proliferation (epithelial cell adhesion molecule). In the assembly of these multicomponent complexes, CD147 and CD98hc play a central organizing role. RNA interference knock-down experiments established a strong connection between CD147 and CD98hc expression and a strong positive association of CD147 (and CD98hc) with cell proliferation. As the CD147-CD98hc complex and proliferation diminished, AMP-activated protein kinase (a cellular “fuel gauge”) became activated, indicating a disturbance of cellular energy metabolism. Our data point to a CD147-CD98 cell surface supercomplex that plays a critical role in energy metabolism, likely by coordinating transport of lactate and amino acids. Furthermore we showed how covalent cross-linking, together with mass spectrometry, can be used to identify closely associated transmembrane proteins. This approach should also be applicable to many other types of transmembrane proteins besides those associated with CD98hc and CD147.
Bacterial lipopolysaccharide acts as an adjuvant to induce autoimmune arthritisin mice

We investigated the ability of lipopolysaccharide (LPS) as an adjuvant to induce autoimmune arthritis. LPS from Escherichia coli was intraperitoneally injected into DBA/1J mice together with the joint cartilage component type II collagen (CII) on day 0. Thereafter, the injection of CII and LPS was continued every 2 weeks up to day 56. The results showed that mice injected with CII plus LPS had signs of arthritis on day 55 and the joint inflammation reached a peak on day 75. Injection of CII or LPS alone induced no arthritis. Histologically, marked oedema of synovium and intense infiltration of inflammatory cells, including neutrophils, were observed 3 days after the onset of joint inflammation. Twenty‐one days later, there were marked proliferation of synovial tissues with many mononulear cells and destruction of cartilage. Anti‐CII immunoglobulin G (IgG) and IgG2a antibodies were markedly produced in mice injected with CII plus LPS. Pronounced secretion of cytokines, including interleukins‐12 and ‐1β, interferon‐γ and tumour necrosis factor‐α, was also observed in these animals. Arthritis was passively transferred into naive syngeneic mice with sera but not with lymphoid cells from mice given CII with LPS. Other types of LPS from Salmonella enteritidis, Salmonella typhimurium and Klebsiella pneumoniae as well as lipid A from E. coli, induced inflammation in joints when administered with CII. Polymixin B sulphate mixed with LPS or lipid A blocked the induction of joint inflammation. These results indicate that LPS appears to play an important role as an adjuvant in the induction of arthritis in which autoimmunity to CII is involved.
Ultrastructural Dynamics of Transendothelial Migration of Lymphocytes Through High Endothelial Venules (HEVs) of the Mucosa Associated Peyer’s Patches

Background and Purpose: Although the pivotal role of high endothelial venules (HEVs) in the migration of leukocytes from the blood into the secondary lymphoid parenchyma is well established, conflicting ideas concerning the cellular dynamics both of leukocytes and endothelial cells throughout the migratory processes have been present. Here we focused specifically on the cellular dynamics of HEVs from Peyer’s patches in an ultrastructural perspective. Materials and Methods: In order to determine the microstructural organization of transendothelial migration we used conventional methods for transmission electron microscopy. Results : Our results indicate that both lymphocytes and endothelial cells are highly active in the processes of transmigration steps, and a series of morphological and cellular alterations can occur depending upon their activity. Various types of cellular protrusions provide a direct contact between luminal lymphocytes and the endothelial cells at the initial phases of the migration. The endothelial protrusions subsequently embrace the lymphocytes and guide them into lymphoid stroma during the transcellular migration. Meanwhile, different sizes of vesicles show different cellular localizations according to their roles. The vesicles which are clustered near the lateral borders and the stand alone ones found only in the abluminal surfaces of endothelial cells might be involved in the paracellular migration. Concurrently, the other types of vesicles were smaller and appeared in the lateral border of the endothelial cells. Different from the clustered and abluminally localized vesicles, they were closely related with plasma membranes. Conclusions : These results indicated that not only adhesion molecules, but also cellular dynamics of leukocytes and endothelial cells regulate the leukocyte traffic into lymphoid stroma or vice versa.
Time for Living and Caring: An Intervention to Make Respite More Effective for Caregivers

This article presents the rationale for and description of a promising intervention, Time for Living and Caring (TLC), designed to enhance the effectiveness of respite services for family caregivers. It is guided by the theoretical principles of the Selective Optimization with Compensation (SOC) model, which individually coaches caregivers on how to assess their personal circumstances, identify their greatest needs and preferences, and engage in goal setting and attainment strategies to make better use of their respite time. Focusing on respite activities that match caregivers' unique needs is likely to result in improved well-being. We report on a pilot study examining TLC's feasibility and potential benefits and how caregivers viewed their participation. While additional research is needed to test and refine the intervention, we need to find more creative ways to enhance respite services.
Psychiatric and social reasons for frequent rehospitalization.

OBJECTIVE The authors attempted to identify factors that commonly contributed to the decision to rehospitalize patients who made heavy use of mental health services. METHODS The case notes of 50 patients with frequent readmissions to the South Australian Mental Health Services over a three-year period were examined to identify which of 15 factors most frequently contributed to hospital readmission. RESULTS Lack of insight or denial of illness was cited in 62.2 percent of the patients' 442 total admissions, followed by relationship problems (61.1 percent), suicidal ideation (44.8 percent), and noncompliance with medication (43.2 percent). When the 15 factors were combined into four major categories, social factors were found to contribute to 38.9 percent of admissions, followed by factors related to psychiatric and physical illness (31.1 percent), dangerousness to self or others (20.3 percent), and substance abuse (9.7 percent). CONCLUSIONS The substantial contribution of social factors to the readmission of patients to acute mental health services is strong evidence that the mental health system must provide appropriate targeted resources and assertive, continuous case management to avoid social crises. Issues surrounding drug and alcohol abuse among heavy users of services must be actively addressed.
Hearing in Drosophila: Development of Johnston's organ and emerging parallels to vertebrate ear development

In this review, I describe recent progress toward understanding the developmental genetics governing formation of the Drosophila auditory apparatus. The Drosophila auditory organ, Johnston's organ, is housed in the antenna. Intriguingly, key genes needed for specification or function of auditory cell types in the Drosophila antenna also are required for normal development or function of the vertebrate ear. These genes include distal‐less, spalt and spalt‐related, atonal, crinkled, nanchung and inactive, and prestin, and their vertebrate counterparts Dlx, spalt‐like (sall), atonal homolog (ath), myosin VIIA, TRPV, and prestin, respectively. In addition, Drosophila auditory neurons recently were shown to serve actuating as well as transducing roles, much like their hair cell counterparts of the vertebrate cochlea. The emerging genetic and physiologic parallels have come as something of a surprise, because conventional wisdom holds that vertebrate and invertebrate hearing organs have separate evolutionary origins. The new findings raise the possibility that auditory organs are more ancient than previously thought and indicate that Drosophila is likely to be a powerful model system in which to gain insights regarding the etiologies of human deafness disorders. Developmental Dynamics 232:550–558, 2005. © 2005 Wiley‐Liss, Inc.
A serum metabonomic study on the difference between alcohol- and HBV-induced liver cirrhosis by ultraperformance liquid chromatography coupled to mass spectrometry plus quadrupole time-of-flight mass spectrometry.

BACKGROUND Liver cirrhosis is the fatal consequence of chronic hepatitis, making early diagnosis of liver cirrhosis critical. Liver biopsy is still the standard diagnostic method for liver cirrhosis, although its use in a broad population with alcoholism or hepatitis B virus (HBV) infection remains difficult. In this study, we used a metabonomic approach to detect potential biomarkers for early diagnosis of liver cirrhosis. METHODS Serum specimens were collected prospectively from normal control subjects (n = 22) and patients with alcoholic cirrhosis (n = 18) or HBV-induced cirrhosis (n = 19). The serum metabonome was analyzed using ultraperformance liquid chromatography (LC)/time-of-flight mass spectrometry (MS) integrated with chemometrics. The acquired LC-MS data were normalized and processed using principal component analysis (PCA) and partial least squares discrimination analysis (PLS-DA). RESULTS Significant differences in the metabonomics among the three groups were observed. Lysophosphatidyl cholines (LPCs) (LPC C16:0, LPC C18:0, LPC C18:2, LPC C18:3, LPC C20:3, LPC C20:5) were decreased in the serum of patients with hepatic cirrhosis, whereas bile acids (glycocholic acid, glycochenodeoxycholic acid), hypoxanthine, and stearamide were increased in the serum of patients with hepatic cirrhosis. These metabolites are considered "common" biomarkers for hepatic cirrhosis. Oleamide and myristamide were increased in the serum of patients with alcoholic cirrhosis but decreased in those with HBV-induced cirrhosis. These could be specific biomarkers for differential diagnosis between alcohol- and HBV-induced hepatic cirrhosis. CONCLUSIONS There are significant metabonomic differences between alcohol- and HBV-induced liver cirrhosis. Metabonomics is a top-down systems biology tool for conducting research on clinical problems.
Quality assessment of computer‐assisted semen analysis (CASA) in the andrology laboratory

If quality is assessed with regard to computer‐assisted semen analysis (CASA), the evaluation of seminal fluid in the andrological laboratory has to be considered. Three levels of quality assessment are generally accepted: structure, process and results. Quality of structure mainly concerns the quality of laboratory assessment, in particular the skill of the staff and the equipment used. The quality of the CASA system itself is difficult to assess. Process quality concerns the quality of performing a diagnosis. When the parameter settings of the CASA system and the handling of the sample are defined, the reproducibility of the CASA values is clearly better than that of the visual estimation of motility. CASA systems are also superior to other methods regarding the documentation of laboratory values, as all the values are obtained directly online. Result quality comprises the precision, reliability and reproducibility of measurement as well as the significance of values with respect to their biological relevance. Concluding from the definitions as quoted above and from reports of the literature it may be stated that: (i) in the dimensions of structure and process quality, CASA is superior to other methods of measuring sperm motility; (ii) the evaluation of results and quality of results, however, is highly problematic; (iii) CASA systems do not appear to be superior to the visual estimation of sperm motility with respect to the fertilizing capacity of spermatozoa; (iv) the guidelines of the WHO task force form a basis for sufficient process quality; (v) further efforts should actually focus not on the improvement of investigation technology, but on the improvement in the qualification of investigators.
Molgula pugetiensis is a Pacific Tailless Ascidian Within the Roscovita Clade of Molgulids

The molgulid ascidians are a monophyletic group of tunicates that share specific morphological features, includ ing a heart/kidney complex and hermaphroditic gonads found on both sides of the adult. The larval body plan, however, is quite plastic, with tailless species evolving at least four times independently within the molgulids. The Roscovita clade of molgulid ascidians includes three spe cies that are found sympatrically in Roscojf, France, and includes both tailed and tailless species. Another species in this clade, Molgula pacifica, is a direct developing tailless molgulid ascidian within the Roscovita clade found on the outer coast of Vancouver Island. M. pacifica "larvae" never hatch, and they undergo metamorphosis within the chorion. Molgula pugetiensis is a molgulid ascidian found in the San Juan Islands, but its development has not been reported. Here, we show that this species, belonging to the Roscovita clade, is a tailless ascidian species with "larvae " that hatch from the chorion before metamorphosis, similar to the well described tailless Molgula occulta from Roscojf, France. Immediately after hatching, the tailless larvae develop am pullae and proceed to metamorphose into juveniles. Tunicates in the Molgulidae are a monophyletic group of solitary ascidians (Ascidiacea: Stolidobranchia) that are sis ter group to the families Styelidae and Pyuridae (1-3). Evidence from 18S rDNA shows that the stolidobranch tunicates may also be closely related to the appendicular ians, which include the pelagic oikopleurid tunicates that build mucous houses (2-4). Molgulidae is an interesting group for research on the evolution of body plans because it contains species that have urodele, tailed, chordate larvae with notochord and muscle as well as closely related species
Isolation of a putative prolyl‐tRNA synthetase (CaPRS) gene from Candida albicans

We have isolated a 4·0‐kb fragment from a genomic library of Candida albicans which contained two open reading frames (ORFs). One of them is homologous to a prolyl‐tRNA synthetase that catalyses the charging of a specific tRNA by proline (CaPRS). A deduced sequence of 575 amino acids representing a polypeptide of 66·2 kDa was determined. A FASTA search indicated that the CaPRSp had an overall similarity of 54·4% with the product of a Saccharomyces cerevisiae ORF (YER087) and 43·8% with the prolyl‐tRNA synthetase of Escherichia coli (COLIPRO). Consensus Class II aminoacyl‐tRNA synthetase sequences were identified by the PROSITE program. CaPRS was localized to chromosome R of the C. albicans genome and CaPRS DNA hybridized to a major RNA transcript of 1·7 kb under all conditions tested. The CaPRS sequence submitted to the EMBL data library is available under Accession Number U86341. © 1997 John Wiley & Sons, Ltd.
Detection of A/B toxin and isolation of Clostridium difficile and Clostridium perfringens from foals.

REASONS FOR PERFORMING THE STUDY Toxin detection and screening could contribute to knowledge of the transmission patterns, risk factors and epidemiology of Clostridium difficile and Clostridium perfringens. OBJECTIVE To isolate C. difficile and C. perfringens and to detect A/B toxins in faecal samples from diarrhoeic and nondiarrhoeic foals. STUDY DESIGN Cross-sectional observational study. METHODS A total of 153 samples from foals were collected: 139 samples from farms and 14 samples from diarrhoeic foals admitted to a veterinary hospital. The A/B toxins were detected by cytotoxicity assay. All suspected colonies of C. perfringens were subjected to polymerase chain reaction for detection of the major toxin genes (α, β, ε and ι) and for detection of β2-, NetB- and enterotoxin-encoding genes. Furthermore, C. difficile and C. perfringens isolates were evaluated for in vitro antimicrobial susceptibility. RESULTS Seven of 153 (4.6%) samples, all from diarrhoeic foals, were positive for C. difficile A/B toxin. Of these, 5 of 14 (35.7%) were from hospitalised foals, and only 2 of 63 (3.2%) diarrhoeic foal samples were from farms (P = 0.002). Clostridium perfringens was isolated from 31 (20.3%) foals, of which 21 of 76 (27.6%) were diarrhoeic and 10 of 76 (13.2%) were nondiarrhoeic, demonstrating a difference between these 2 groups (P = 0.045). Only 4 strains were positive for the β2-encoding gene (cpb2). All C. difficile and C. perfringens isolates were susceptible to metronidazole and vancomycin. CONCLUSIONS The present report highlights the need for laboratory diagnostics to differentiate C. difficile-associated infection in foals from other causes of diarrhoea to facilitate adequate antimicrobial therapy. POTENTIAL RELEVANCE More studies are needed to clarify the role of C. perfringens as a primary agent of diarrhoea in foals.
Power allocation in cognitive MISO wireless networks

In this paper, a spectrum sharing cognitive radio(CR) multiple input and single output (MISO) system is considered, which consists of a secondary user (SU) having multiple transmit antennas and a single receive antenna and a primary user (PU) have a single receive antenna. The channel state information (CSI) on the link between SU and PU is assumed to be perfectly known at the SU transmitter (SU-Tx). Such a system is subject to interference constraint and transmit power constraint. We formulate multi-constraint optimization problem in its equivalent form, and joint beamforming (BF) and power allocation to obtain the maximization capacity.
Onset of psoriasis with psoriatic arthropathy during rituximab treatment of non-Hodgkin lymphoma

We have monitored a 66-year-old woman who was diagnosed with non-Hodgkin’s lymphoma (NHL) based on a biopsy of her right breast in June 2006. Her previous including family history was unremarkable. Following administration of the first two cycles of rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP), she developed asymmetric arthritis that affected the distal interphalangeal joints, the left shoulder and ankles with achillodynia in July/August 2006. Of note, these manifestations emerged within the first 6–8 weeks following the first rituximab exposure. Initially, acute gouty arthritis was suspected following chemotherapy, but normal levels of uric acid were found. She did not respond sufficiently to non-steroidal anti-inflammatory drugs (NSAIDs) and allopurinole and her arthritis progressed. Subsequently, widespread arthritis and …
Cortical functional reorganization and its relationship with brain structural damage in patients with benign multiple sclerosis

Background: Patients with multiple sclerosis (MS) who have a favourable clinical status several years after disease onset are classified as ‘benign’. In many cases brain tissue damage does not differ between benign MS and the ‘classical’ MS forms. Objective: To assess whether the favourable clinical course in benign MS could be explained by the presence of an efficient functional cortical reorganization. Method: Twenty-five right-handed patients with benign MS (defined as having Expanded Disability Status Scale ≤ 3 and disease duration >15 years) underwent functional MRI during a simple motor task (right-hand tapping) to assess movement-associated brain activation. This was compared with that of 10 patients with relapsing—remitting MS and 10 normal controls. Benign MS patients also underwent conventional brain MRI and magnetization transfer imaging, which was compared with an identical examination obtained 1 year before. Quantitative structural magnetic resonance measures were baseline and changes over time in T2-lesion volume, magnetization transfer ratio in T2 lesions and normal-appearing brain and total brain volume. Results: Movement-related activation was greater in patients with benign MS than in those with relapsing—remitting MS or normal controls, extensively involving bilateral regions of the sensorimotor network as well as basal ganglia, insula and cerebellum. Greater activation correlated with lower T2-lesion magnetization transfer ratio, and with decreasing brain volume and increasing T2 lesion volume. Conclusions: The results suggest that bilateral brain networks, beyond those normally engaged in motor tasks, are recruited during a simple hand movement in patients with benign MS. This increased activation is probably the expression of an extensive, compensatory and tissue-damage related functional cortical reorganization. This can explain, at least in part, the favourable clinical expression of patients with benign MS.
Submicroscopic genomic rearrangements change gene expression in T‐cell large granular lymphocyte leukemia

To better understand the molecular pathogenesis of T‐cell large granular lymphocyte leukemia (T‐LGL), we decided to search for those genetic alterations in T‐LGL patients and MOTN‐1 cell line (established from T‐LGL patient) that have an impact on gene expression and as a result can influence cell biology.