Miracle-dz/UniFAR-FTUs huggingface dataset:
Rumen fermentation and liveweight gain in beef cattle treated with monensin and grazing lush forage.

OBJECTIVE To determine the prevalence of subacute rumen acidosis (SARA) in beef cattle grazing lush pasture and the effect of monensin on reducing SARA and improving animal performance. DESIGN Commercial Angus and Murray Grey steers received a monensin slow-release capsule (n = 19) or remained untreated (n = 19). Cattle grazed an oats crop or tetraploid ryegrass pasture for a total of 91 days. Rumen fluid pH, volatile fatty acids (VFA) and lactic acid concentrations and body weight data were collected prior to treatment and again 28, 56 and 91 days after treatment. Changes in measures over time were analysed using mixed model repeated measures analysis. Differences in average daily gain between treatment groups were determined. RESULTS The prevalence of SARA was low during the study, with only one animal satisfying criteria for SARA at one time point. Cattle treated with monensin capsules were 11.9 kg heavier at the completion of the study compared with untreated controls (414.5 ± 3.88 kg vs 402.6 ± 4.03 kg, P = 0.04). Rumen VFA and L- and D-lactate levels did not differ between cattle treated with monensin and untreated cattle. However, the ratio of propionate to acetate plus two times butyrate was higher (P < 0.001) when cattle were treated with monensin. CONCLUSIONS Subacute rumen acidosis was not consistently detected under the conditions of the study. The higher body weight of cattle treated with monensin may have been due to improved energy utilisation of the pasture, indicated by increased propionate proportions in the rumen, rather than prevention of SARA.
Electroacupuncture Promotes the Differentiation of Transplanted Bone Marrow Mesenchymal Stem Cells Overexpressing TrkC into Neuron-Like Cells in Transected Spinal Cord of Rats

Our previous study indicated that electroacupuncture (EA) could increase neurotrophin-3 (NT-3) levels in the injured spinal cord, stimulate the differentiation of transplanted bone marrow mesenchymal stem cells (MSCs), and improve functional recovery in the injured spinal cord of rats. However, the number of neuron-like cells derived from the MSCs is limited. It is known that NT-3 promotes the survival and differentiation of neurons by preferentially binding to its receptor TrkC. In this study, we attempted to transplant TrkC gene-modified MSCs (TrkC-MSCs) into the spinal cord with transection to investigate whether EA treatment could promote NT-3 secretion in the injured spinal cord and to determine whether increased NT-3 could further enhance transplanted MSCs overexpressing TrkC to differentiate into neuron-like cells, resulting in increased axonal regeneration and functional improvement in the injured spinal cord. Our results showed that EA increased NT-3 levels; furthermore, it promoted neuron-phenotype differentiation, synaptogenesis, and myelin formation of transplanted TrkC-MSCs. In addition, TrkC-MSC transplantation combined with EA (the TrkC-MSCs + EA group) treatment promoted the growth of the descending BDA-labeled corticospinal tracts (CSTs) and 5-HT-positive axonal regeneration across the lesion site into the caudal cord. In addition, the conduction of cortical motor-evoked potentials (MEPs) and hindlimb locomotor function increased as compared to controls (treated with the LacZ-MSCs, TrkC-MSCs, and LacZ-MSCs + EA groups). In the TrkC-MSCs + EA group, the injured spinal cord also showed upregulated expression of the proneurogenic factors laminin and GAP-43 and downregulated GFAP and chondroitin sulfate proteoglycans (CSPGs), major inhibitors of axonal growth. Together, our data suggest that TrkC-MSC transplantation combined with EA treatment spinal cord injury not only increased MSC survival and differentiation into neuron-like cells but also promoted CST regeneration across injured sites to the caudal cord and functional improvement, perhaps due to increase of NT-3 levels, upregulation of laminin and GAP-43, and downregulation of GFAP and CSPG proteins.
Cooperation of adapter molecules in proximal signaling cascades during allergic inflammation

Summary: Activation of mast cells through their high‐affinity immunoglobulin E receptor (FcεRI) plays an important role in allergic disorders. Other mast cell‐activating stimuli, such as Toll‐like receptor (TLR) ligands, synergize with FcεRI to enhance allergic inflammation. Thus, there is much interest in understanding how signaling occurs downstream of these receptors. One key event for FcεRI‐mediated mast cell activation is the inducible formation of multimolecular proximal signaling complexes. These complexes are nucleated by adapter proteins, scaffolds that localize various signaling molecules through their multiple molecule‐binding domains. Here we review recent findings in proximal signaling cascades with an emphasis on how adapter molecules cooperate with each other to generate an optimal signal in mast cells, and we discuss how signals crosstalk between FcεRI and TLRs in enhancing mast cell activation. Deciphering the molecular mechanisms leading to mast cell activation will hopefully bring new ideas for the development of novel therapeutics to control allergic diseases.
A Comparative Study of Variables That Have an Impact on Noncancer End-of-Life Diagnoses

By way of analysis of variance, this secondary analysis compared three groups of noncancer home hospice patients (AIDS, amyotrophic lateral sclerosis [ALS],and other) for psychological correlates of adaptation and well-being, pain, and physical function, as measured by the Life Closure Scale (LCS), the Affect Balance Scale, the McGill-Melzack Pain Questionnaire Part I, and the Karnofsky Performance Status Scale. The sample included 10 AIDS patients (Group 1), 6 ALS patients (Group 2), and 5 patients with other noncancer, end-stage diseases (Group 3). A significant difference was found between Group 2 (ALS) and Group 3 (other) on the LCS, F(2, 18) = 4.3, p = . 03. Scheffe and Duncan range post hoc tests and at test at the p≤. 05 level (t9 = 4.1, p = .003) confirmed the finding. No significant group differences were found among the ALS, AIDS, and other groups in measures of psychological well-being, pain, and physical function.
Supplementary motor area stimulation for Parkinson disease

Objective: To explore the efficacy and stimulation frequency dependence of repetitive transcranial magnetic stimulation (rTMS) over the supplementary motor area (SMA) in Parkinson disease (PD). Methods: In this randomized, double-blind, sham-controlled, multicenter study with a parallel design, a weekly intervention was performed 8 times. The effects were monitored up to 20 weeks. By central registration, participants were assigned to 1 of 3 arms of the study: low-frequency (1-Hz) rTMS, high-frequency (10-Hz) rTMS, and realistic sham stimulation. The primary end point was the score change of the Unified Parkinson's Disease Rating Scale (UPDRS) part III from the baseline. Several nonmotor symptom scales such as the Hamilton Rating Scale for Depression, apathy score, and nonmotor symptoms questionnaire were defined as secondary end points. Results: Of the 106 patients enrolled, 36 were allocated to 1-Hz rTMS, 34 to 10-Hz rTMS, and 36 to realistic sham stimulation. Results show 6.84-point improvement of the UPDRS part III in the 1-Hz group at the last visit of the 20th week. Sham stimulation and 10-Hz rTMS improved motor symptoms transiently, but their effects disappeared in the observation period. Changes in nonmotor symptoms were not clear in any group. No severe adverse event was reported. Conclusions: The 1-Hz rTMS over the SMA was effective for motor, but not nonmotor, symptoms in PD. Level of evidence: This study provides Class I evidence that 1-Hz rTMS over the SMA is effective for motor symptoms in PD.
4D CT amplitude binning for the generation of a time-averaged 3D mid-position CT scan

The purpose of this study was to develop a method to use amplitude binned 4D-CT (A-4D-CT) data for the construction of mid-position CT data and to compare the results with data created from phase-binned 4D-CT (P-4D-CT) data. For the latter purpose we have developed two measures which describe the regularity of the 4D data and we have tried to correlate these measures with the regularity of the external respiration signal. 4D-CT data was acquired for 27 patients on a combined PET-CT scanner. The 4D data were reconstructed twice, using phase and amplitude binning. The 4D frames of each dataset were registered using a quadrature-based optical flow method. After registration the deformation vector field was repositioned to the mid-position. Since amplitude-binned 4D data does not provide temporal information, we corrected the mid-position for the occupancy of the bins. We quantified the differences between the two mid-position datasets in terms of tumour offset and amplitude differences. Furthermore, we measured the standard deviation of the image intensity over the respiration after registration (σregistration) and the regularity of the deformation vector field (Δ|J|¯ ? >) to quantify the quality of the 4D-CT data. These measures were correlated to the regularity of the external respiration signal (σsignal). The two irregularity measures, Δ|J|¯ ? > and σregistration, were dependent on each other (p < 0.0001, R2 = 0.80 for P-4D-CT, R2 = 0.74 for A-4D-CT). For all datasets amplitude binning resulted in lower Δ|J|¯ ? > and σregistration and large decreases led to visible quality improvements in the mid-position data. The quantity of artefact decrease was correlated to the irregularity of the external respiratory signal. The average tumour offset between the phase and amplitude binned mid-position without occupancy correction was 0.42 mm in the caudal direction (10.6% of the amplitude). After correction this was reduced to 0.16 mm in caudal direction (4.1% of the amplitude). Similar relative offsets were found at the diaphragm. We have devised a method to use amplitude binned 4D-CT to construct motion model and generate a mid-position planning CT for radiotherapy treatment purposes. We have decimated the systematic offset of this mid-position model with a motion model derived from P-4D-CT. We found that the A-4D-CT led to a decrease of local artefacts and that this decrease was correlated to the irregularity of the external respiration signal.
National patterns and predictors of beta-blocker use in patients with coronary artery disease.

BACKGROUND Prior studies suggest underuse of beta-blockers in patients with coronary artery disease, but these studies have been based on selected populations of recently hospitalized patients. OBJECTIVE To describe national patterns and determinants of beta-blocker use in the ambulatory setting. METHODS We analyzed 11745 visits by patients with coronary artery disease to randomly selected, office-based physicians in the National Ambulatory Medical Care Surveys for 1980, 1981, 1985, and 1989 through 1996. We used multiple logistic regression to determine the independent effect of sociodemographic and clinical factors on beta-blocker use. OUTCOME MEASURE Beta-blocker use at patient visits. RESULTS Beta-blocker use was reported in only 20.9% of office visits by patients with coronary artery disease and no strong contraindications between 1993 and 1996. In multivariate analyses, age younger than 75 years, residence in the Northeast, and visits to cardiologists and internists compared with family and general practitioners predicted greater use of beta-blocker therapy. White race and private insurance also were significant predictors of beta-blocker use between 1980 and 1996. Longitudinal analyses revealed a significant decline in beta-blocker use from 1980 to 1990, followed by a gradual increase in recent years. CONCLUSIONS Beta-blockers appear to be underused in ambulatory patients with coronary artery disease. Our data suggest that nonclinical factors may influence rates of use, indicating the need for closer scrutiny of variations in physician prescribing practices.
Hydrodynamics of spur and groove formations on a coral reef

[1] Spur and groove (SAG) formations are found on the fore reefs of many coral reefs worldwide. Although these formations are primarily present in wave-dominated environments, their effect on wave-driven hydrodynamics is not well understood. A two-dimensional, depth-averaged, phase-resolving nonlinear Boussinesq model (funwaveC) was used to model hydrodynamics on a simplified SAG system. The modeling results show that the SAG formations together with shoaling waves induce a nearshore Lagrangian circulation pattern of counter-rotating circulation cells. The mechanism driving the modeled flow is an alongshore imbalance between the pressure gradient (PG) and nonlinear wave (NLW) terms in the momentum balance. Variations in model parameters suggest the strongest factors affecting circulation include spur-normal waves, increased wave height, weak alongshore currents, increased spur height, and decreased bottom drag. The modeled circulation is consistent with a simple scaling analysis based on the dynamical balance of NLW, PG, and bottom stress terms. Model results indicate that the SAG formations efficiently drive circulation cells when the alongshore SAG wavelength allows for the effects of diffraction to create alongshore differences in wave height without changing the mean wave angle.
Endoscopic Sphincterotomy and Recurrence of Acute Pancreatitis in Gallstone Patients Considered Unfit for Surgery

The aim of the present prospective study was to investigate whether endoscopic sphincterotomy may be useful in preventing recurrence of acute pancreatitis in patients with gallstones and a high anesthesiological risk of cholecystectomy. Twenty-six elderly patients with severe cardiopulmonary, hepatic, and renal diseases were considered. Endoscopic sphincterotomy was successful in 19 cases (group A: mean age, 78.4 years; range, 71–87) and failed in 7 patients (group B: mean age, 79.8 years; range 73–85). In the follow-up period biliary pain without an increase in pancreatic serum enzymes occurred in six patients in group A and in all patients in group B (p = 0.002); recurrence of acute biliary pancreatitis with a need for hospitalization occurred in one patient in group A and in four patients in group B (p = 0.01). These results suggest that endoscopic sphincterotomy may be considered a very useful option in reducing the recurrence of acute biliary pancreatitis in elderly patients with gallstones and a high anesthesiological risk of cholecystectomy.
THE HUMAN INTESTINAL CYTOCHROME P450 “PIE”

Cytochromes P450 (P450s) 3A, 2C, and 1A2 constitute the major “pieces” of the human liver P450 “pie” and account, on average, for 40, 25, and 18%, respectively, of total immunoquantified P450s (J Pharmacol Exp Ther 270:414–423, 1994). The P450 profile in the human small intestine has not been fully characterized. Therefore, microsomes prepared from mucosal scrapings from the duodenal/jejunal portion of 31 human donor small intestines were analyzed by Western blot using selective P450 antibodies. P450s 3A4, 2C9, 2C19, and 2J2 were detected in all individuals and ranged from 8.8 to 150, 2.9 to 27, <0.6 to 3.9, and <0.2 to 3.1 pmol/mg, respectively. CYP2D6 was detected in 29 individuals and ranged from <0.2 to 1.4 pmol/mg. CYP3A5 was detected readily in 11 individuals, with a range (average) of 4.9 to 25 (16) pmol/mg that represented from 3 to 50% of total CYP3A (CYP3A4 + CYP3A5) content. CYP1A1 was detected readily in three individuals, with a range (average) of 3.6 to 7.7 (5.6) pmol/mg. P450s 1A2, 2A6, 2B6, 2C8, and 2E1 were not or only faintly detected. As anticipated, average CYP3A content (50 pmol/mg) was the highest. Excluding CYP1A1, the remaining enzymes had the following rank order: 2C9 > 2C19 > 2J2 > 2D6 (8.4, 1.1, 0.9, and 0.5 pmol/mg, respectively). Analysis of a pooled preparation of the 31 donor specimens substantiated these results. In summary, as in the liver, large interindividual variation exists in the expression levels of individual P450s. On average, CYP3A and CYP2C9 represents the major pieces of the intestinal P450 pie, accounting for 80 and 15%, respectively, of total immunoquantified P450s.
Interleukin 1 enhances T-dependent immune responses by amplifying the function of dendritic cells

The function of exogenous murine recombinant IL-1 alpha as a T lymphocyte-activating molecule was examined. IL-1 did not induce IL-2 release or responsiveness in purified T cells regardless of their state of activation: unprimed lymphocytes, freshly sensitized lymphocytes, or memory cells derived from the blasts. Nor did IL-1 synergize with mitogens, or with antigens, to stimulate proliferation. For example the combinations of IL-1 plus Ia+ peritoneal macrophages, or IL-1 plus Con A, were less than 5% as effective in triggering T cell growth as a low dose (1%) of dendritic cells. However, when IL-1 was added at the onset of culture, the response to limiting doses of dendritic cells was increased 3- to 10-fold in several systems: the syngeneic and allogeneic MLR, Con A- and periodate-induced polyclonal mitogenesis, and T-dependent antibody formation against foreign red cells. The amplifying effect of IL-1 could be obtained if the dendritic cells but not the responding lymphocytes were exposed to IL-1 before use as accessory cells. Optimal activation of dendritic cells required a dose of 5 U/ml (50 pM) and 18 h of exposure, and was not due to carryover of IL-1 into the lymphocyte culture. IL-2, IL-3, and cachectin/TNF did not amplify dendritic cell function, while IFN-gamma diminished it. The enhanced function of IL-1-treated dendritic cells was due to an enhanced clustering with helper T lymphocytes in the first day of the MLR response. Therefore IL-1 does not seem to act as an activating factor for most peripheral T lymphocytes. Instead, IL-1 enhances the function of accessory dendritic cells and represents the first molecule that has been shown to enhance the immune response at this critical level.
Longitudinal quantification and visualization of intracerebral haemorrhage using multimodal magnetic resonance and diffusion tensor imaging

Abstract Objective: To demonstrate a set of approaches using diffusion tensor imaging (DTI) tractography whereby pathology-affected white matter (WM) fibres in patients with intracerebral haemorrhage (ICH) can be selectively visualized. Methods: Using structural neuroimaging and DTI volumes acquired longitudinally from three representative patients with ICH, the spatial configuration of ICH-related trauma is delineated and the WM fibre bundles intersecting each ICH lesion are identified and visualized. Both the extent of ICH lesions as well as the proportion of WM fibres intersecting the ICH pathology are quantified and compared across subjects. Results: This method successfully demonstrates longitudinal volumetric differences in ICH lesion load and differences across time in the percentage of fibres which intersect the primary injury. Conclusions: Because neurological conditions such as intracerebral haemorrhage (ICH) frequently exhibit pathology-related effects which lead to the exertion of mechanical pressure upon surrounding tissues and, thereby, to the deformation and/or displacement of WM fibres, DTI fibre tractography is highly suitable for assessing longitudinal changes in WM fibre integrity and mechanical displacement.
Nonlinear System Identification Using a Subband Adaptive Volterra Filter

This paper presents a flexible and efficient subband adaptive second-order Volterra filter (SBVF) structure for nonlinear system identification. The structure is first described in detail, where the underlying filter-bank scheme and adaptive filtering algorithms are explained, followed by a computational complexity analysis. Simulation results are then presented, showing that the proposed structure can achieve equal system-identification performance compared with that of a fullband second-order Volterra structure at a much-reduced complexity. In addition, the structure provides a more precise system model compared with that of a linear-only structure at a potentially similar computational expense. The results also demonstrate the suggested structure's ability to exploit a priori knowledge of the nature of the system nonlinearity through selectable nonlinear subband filtering, resulting in further complexity savings. The simulation results are experimentally verified under a practical acoustic-echo-cancellation scenario. It is shown that the SBVF structure can achieve up to a 10-dB lower mean-square error than that of a linear-only model at a comparable complexity.
Personality scale validities increase throughout medical school.

Admissions and personnel decisions rely on stable predictor-criterion relationships. The authors studied the validity of Big Five personality factors and their facets for predicting academic performance in medical school across multiple years, investigating whether criterion-related validities change over time. In this longitudinal investigation, an entire European country's 1997 cohort of medical students was studied throughout their medical school career (Year 1, N = 627; Year 7, N = 306). Over time, extraversion, openness, and conscientiousness factor and facet scale scores showed increases in operational validity for predicting grade point averages. Although there may not be any advantages to being open and extraverted for early academic performance, these traits gain importance for later academic performance when applied practice increasingly plays a part in the curriculum. Conscientiousness, perhaps more than any other personality trait, appears to be an increasing asset for medical students: Operational validities of conscientiousness increased from . 18 to . 45. In assessing the utility of personality measures, relying on early criteria might underestimate the predictive value of personality variables. Implications for personality measures to predict work performance are discussed.
Menopausal symptom experience: an online forum study.

AIM The aim of the study was to explore the menopausal symptom experience of White midlife women. BACKGROUND Recent cross-cultural investigations have indicated important ethnic differences in menopausal symptoms and have challenged the universality of these symptoms. Currently available cultural knowledge on menopausal experience, however, is inadequate to guide appropriate and adequate care even for White women in the menopausal transition. METHOD A cross-sectional qualitative online forum study was conducted in 2007 with 23 midlife women who self-identified as non-Hispanic Whites using convenience sampling. Seven topics related to menopausal symptom experience were used to guide the online forum for 6 months. The data were analysed using thematic analysis. RESULTS The experiences of menopause caused women to redefine themselves within their busy daily life schedules. They were optimistic about their symptoms, and tried to laugh at the experience to boost their inner strength and motivate themselves to persevere. Many thought that both generational and life-style differences were much more important than ethnic differences in menopausal symptom experiences. In seeking assistance with the symptoms of menopause, women were not satisfied with the guidance of their physicians. CONCLUSION Nurses need to listen carefully to what women themselves say about their own experiences with menopausal symptoms and avoid imposing predetermined symptom management strategies. Further studies of women's perceptions of life-style influences are needed to guide symptom management. In addition, studies of generational changes in menopausal symptom experience are needed.