Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Malva sylvestris and Its Isolated Polysaccharide on Experimental Ulcerative Colitis in Rats

Malva sylvestris is an edible plant that is consumed as a herbal supplement for its antiulcer and colon cleansing properties in traditional Persian medicine. This study was designed to evaluate its effects on ulcerative colitis, which is a chronic gastrointestinal inflammation. Colitis was induced by rectal instillation of acetic acid solution. Rats in different groups received aqueous, n-hexane, or ethanolic fractions of the plant before induction of colitis. Isolated polysaccharide of plant was also tested in 2 groups before and after induction of colitis. Macroscopic and microscopic evaluation of colitis showed that the aqueous fraction was very effective in preventing the inflammation and efficacy was lower for ethanolic and n-hexane fractions. Polysaccharide was effective in reducing signs of inflammation, especially as pretreatment. These beneficial effects provide evidences that this plant can be suggested for patients with this disease to improve their health condition or to reduce adverse effects of their medication.
Effectiveness of a Pilot Mindfulness Program in Volunteers of a Breast Cancer Association

It has been usually observed that medical and health personnel, volunteers, and social workers who work with cancer and chronic patients may have higher rates of compassion fatigue and symptoms of anxiety and depression. The purpose of this study was to examine the benefits of a pilot mindfulness program in a sample of 7 volunteers of a foundation that supports women with breast cancer. The variables analyzed were depression, anxiety, compassion fatigue, work stress, and negative and positive affect. The results showed that the mindfulness program had a positive impact, reducing the symptoms of anxiety and depression and increasing emotional well-being. Participants also reported that the program helped them be more relaxed, identify their emotions, enjoy their time and environment better, increasing the value of life, and facilitate communication among the volunteers. The program produced positive changes in participants, powering personal areas and increasing emotional well-being.
Distinguishing Core and Holoenzyme Mechanisms of Transcription Termination by RNA Polymerase III

ABSTRACT Transcription termination by RNA polymerase (Pol) III serves multiple purposes; it delimits interference with downstream genes, forms 3′ oligo(U) binding sites for the posttranscriptional processing factor, La protein, and resets the polymerase complex for reinitiation. Although an interplay of several Pol III subunits is known to collectively control these activities, how they affect molecular function of the active center during termination is incompletely understood. We have approached this using immobilized Pol III-nucleic acid scaffolds to examine the two major components of termination, transcription pausing and RNA release. This allowed us to distinguish two mechanisms of termination by isolated Saccharomyces cerevisiae Pol III. A core mechanism can operate in the absence of C53/37 and C11 subunits but requires synthesis of 8 or more 3′ U nucleotides, apparently reflecting inherent sensitivity to an oligo(rU·dA) hybrid that is the termination signal proper. The holoenzyme mechanism requires fewer U nucleotides but uses C53/37 and C11 to slow elongation and prevent terminator arrest. N-terminal truncation of C53 or point mutations that disable the cleavage activity of C11 impair their antiarrest activities. The data are consistent with a model in which C53, C37, and C11 activities are functionally integrated with the active center of Pol III during termination.
The p107/E2F Pathway Regulates Fibroblast Growth Factor 2 Responsiveness in Neural Precursor Cells

ABSTRACT We have previously shown that p107, a member of the retinoblastoma (Rb) cell cycle regulatory family, has a unique function in regulating the pool of neural precursor cells. As the pool of progenitors is regulated by a limiting supply of trophic factors, we asked if the Rb/E2F pathway may control the size of the progenitor population by regulating the levels of growth factors or their receptors. Here, we demonstrate that fibroblast growth factor 2 (FGF2) is aberrantly upregulated in the brains of animals lacking Rb family proteins and that the gene encoding the FGF2 ligand is directly regulated by p107 and E2F3. Chromatin immunoprecipitation assays demonstrated that E2F3 and p107 occupy E2F consensus sites on the FGF2 promoter in the context of native chromatin. To evaluate the physiological consequence of FGF2 deregulation in both p107 and E2F3 mutants, we measured neural progenitor responsiveness to growth factors. Our results demonstrate that E2F3 and p107 are each mediators of FGF2 growth factor responsiveness in neural progenitor cells. These results support a model whereby p107 regulates the pool of FGF-responsive progenitors by directly regulating FGF2 gene expression in vivo. By identifying novel roles for p107/E2F in regulating genes outside of the classical cell cycle machinery targets, we uncover a new mechanism whereby Rb/E2F mediates proliferation through regulating growth factor responsiveness.
An Rtt109-Independent Role for Vps75 in Transcription-Associated Nucleosome Dynamics

ABSTRACT The histone chaperone Vps75 forms a complex with, and stimulates the activity of, the histone acetyltransferase Rtt109. However, Vps75 can also be isolated on its own and might therefore possess Rtt109-independent functions. Analysis of epistatic miniarray profiles showed that VPS75 genetically interacts with factors involved in transcription regulation whereas RTT109 clusters with genes linked to DNA replication/repair. Additional genetic and biochemical experiments revealed a close relationship between Vps75 and RNA polymerase II. Furthermore, Vps75 is recruited to activated genes in an Rtt109-independent manner, and its genome-wide association with genes correlates with transcription rate. Expression microarray analysis identified a number of genes whose normal expression depends on VPS75. Interestingly, histone H2B dynamics at some of these genes are consistent with a role for Vps75 in histone H2A/H2B eviction/deposition during transcription. Indeed, reconstitution of nucleosome disassembly using the ATP-dependent chromatin remodeler Rsc and Vps75 revealed that these proteins can cooperate to remove H2A/H2B dimers from nucleosomes. These results indicate a role for Vps75 in nucleosome dynamics during transcription, and importantly, this function appears to be largely independent of Rtt109.
VisMOOC: Visualizing video clickstream data from Massive Open Online Courses

Massive Open Online Courses (MOOCs) platforms are becoming increasingly popular in recent years. With thousands of students watching course videos, enormous amounts of clickstream data are produced and recorded by the MOOCs platforms for each course. Such large-scale data provide a great opportunity for instructors and educational analysts to gain insight into online learning behaviors on an unprecedented scale. Nevertheless, the growing scale and unique characteristics of the data also pose a special challenge for effective data analysis. In this paper, we introduce VisMOOC, a visual analytic system to help analyze user learning behaviors by using video clickstream data from MOOC platforms. We work closely with the instructors of two Coursera courses to understand the data and collect task analysis requirements. A complete user-centered design process is further employed to design and develop VisMOOC. It includes three main linked views: the List View to show an overview of the clickstream differences among course videos, the Content-based View to show temporal variations in the total number of each type of click action along the video timeline, the Dashboard View to show various statistical information such as demographic information and temporal information. We conduct two case studies with the instructors to demonstrate the usefulness of VisMOOC and discuss new findings on learning behaviors.
Exploring Smoking Cessation Attitudes, Beliefs, and Practices in Occupational Health Nursing

The purpose of this study was to explore occupational health nurses’ attitudes, beliefs, and practices regarding the delivery of smoking cessation services to workers. The study included 707 members of the American Association of Occupational Health Nurses (AAOHN) who completed a one-time survey during the fall of 2012. Results indicated that occupational health nurses believed that evidence-based treatments are at least somewhat effective and that they should provide smoking cessation services to their workers; however, a majority of occupational health nurses reported that they did not have appropriate smoking cessation training or guidelines in their workplaces. Occupational health nurses would benefit from training in the use of smoking cessation guidelines and evidence-based smoking cessation interventions, which could be used in their clinical practice. Employers should ensure that workplace policies, such as providing coverage for cessation services, facilitate smokers’ efforts to quit. Employers can benefit from many of these policies through cost savings via reduced health care costs and absenteeism.
A study of the factors affecting the likelihood of patients participating in a campaign to improve staff hand hygiene

his study aimed to seek patient opinion on being asked to participate in a campaign to improve staff compliance with handwashing and to identify factors that may influence the likelihood of patients asking staff to wash their hands. A descriptive survey of 200 patients (150 participants, response rate 75 per cent) about to be discharged from an acute NHS Trust was completed. The age, gender, previous experience, opinions and personality traits of participants were explored. The results showed that 118 (79 per cent) thought they should be involved in helping staff improve hand hygiene and that extraversion was the only personality trait with a significant association with this tendency of wanting to be involved. Age was a significant factor in how likely participants were to ask different groups of staff and how they would feel about asking, with older people being more reluctant. Participants valued measures that facilitated them asking — staff wearing badges was the most popular of these. Groups of patients in this and other studies (National Patient Safety Agency, 2004) indicate that patients want to be involved in helping to improve hand hygiene. In practice, we need to acknowledge the complex nature of involving individuals in challenging staff to improve their own care, and also that hand hygiene is ultimately the responsibility of health professionals.
Employment Maintenance and Intimate Partner Violence

Intimate partner violence (IPV) is a major public health problem in the United States. Negative outcomes of IPV affect women’s attainment and maintenance of employment. The purpose of this study was to develop a theoretical framework that described and explained the process by which women who have experienced IPV attain and maintain employment. Grounded theory methodology was used to analyze interviews of 34 women who had experienced IPV. Analysis suggested that women who had experienced IPV could attain employment; however, they had difficulty maintaining employment. Entanglement of work and IPV was experienced by all 34 participants because of the perpetrator controlling their appearance, sabotaging their work, interfering with their work, or controlling their finances. Some women described ways in which they disentangled work from IPV through a dynamic unraveling process, with periods of re-entanglement, resulting in job security and satisfaction.
Work-Related Low Back Pain Among Physical Therapists in Riyadh, Saudi Arabia

Low back pain (LBP) is a common health problem. Professions like physical therapy (PT), involving frequent lifting, bending, or standing, are at risk for developing LBP. The objective of this study was to determine the prevalence of work-related LBP and factors associated with and consequences of work-related LBP among physical therapists in Riyadh, Saudi Arabia. A self-administered online questionnaire (i.e., demographic data, history of LBP before and after working as a physical therapist, work setting, and effect on daily activities) was sent to 600 members of the Saudi PT association. Data were analyzed using the Pearson chi-square test and Mann-Whitney U test. Eighty-eight percent of potential respondents completed the questionnaire. Of these, 89.65% of the therapists reported LBP after beginning their PT practice, and 35.6% reported LBP at the time of this survey. Gender, PT specialty, and duration of contact with patients were all found to be related to LBP. The prevalence of work-related LBP among physical therapist in Riyadh was high, affecting patient care and daily activities of the therapists. Both primary and secondary prevention strategies (e.g., introduce ergonomics into PT curricula, reduce therapist stress, and promote teamwork) are needed to decrease LBP among therapists, so they can effectively care for patients.
Ku Regulates Signaling to DNA Damage Response Pathways through the Ku70 von Willebrand A Domain

ABSTRACT The Ku heterodimer (Ku70/Ku80) is a main component of the nonhomologous end-joining (NHEJ) pathway that repairs DNA double-strand breaks (DSBs). Ku binds the broken DNA end and recruits other proteins to facilitate the processing and ligation of the broken end. While Ku interacts with many proteins involved in DNA damage/repair-related functions, few interactions have been mapped to the N-terminal von Willebrand A (vWA) domain, a predicted protein interaction domain. The mutagenesis of Ku70 vWA domain S155/D156 unexpectedly increased cell survival following ionizing radiation (IR) treatment. DNA repair appeared unaffected, but defects in the activation of apoptosis and alterations in the DNA damage signaling response were identified. In particular, Ku70 S155A/D156A affected the IR-induced transcriptional response of several activating transcription factor 2 (ATF2)-regulated genes involved in apoptosis regulation. ATF2 phosphorylation and recruitment to DNA damage-induced foci was increased in Ku70-deficient cells, suggesting that Ku represses ATF2 activation. Ku70 S155A/D156A substitutions further enhanced this repression. S155A substitution alone was sufficient to confer enhanced survival, whereas alteration to a phosphomimetic residue (S155D) reversed this effect, suggesting that S155 is a phosphorylation site. Thus, these findings infer that Ku links signals from the DNA repair machinery to DNA damage signaling regulators that control apoptotic pathways.
Intelligent driver drowsiness detection through fusion of yawning and eye closure

Driver drowsiness is a major factor in most driving accidents. In this paper we present a robust and intelligent scheme for driver drowsiness detection employing the fusion of eye closure and yawning detection methods. In this approach, the driver's facial appearance is captured via a camera installed in the car. In the first step, the face region is detected and tracked in the captured video sequence utilizing computer vision techniques. Next, the eye and mouth areas are extracted from the face; and they are studied to find signs of driver fatigue. Finally, in a fusion phase the driver state is determined and a warning message is sent to the driver if the drowsiness is detected. Our experiments prove the high efficiency of the proposed idea.
CD2AP Regulates SUMOylation of CIN85 in Podocytes

ABSTRACT Podocytes are highly differentiated and polarized epithelial cells located on the visceral side of the glomerulus. They form an indispensable component of the glomerular filter, the slit diaphragm, formed by several transmembrane proteins and adaptor molecules. Disruption of the slit diaphragm can lead to massive proteinuria and nephrotic syndrome in mice and humans. CD2AP is an adaptor protein that is important for the maintenance of the slit diaphragm. Together with its paralogue, CIN85, CD2AP belongs to a family of adaptor proteins that are primarily described as being involved in endocytosis and downregulation of receptor tyrosine kinase activity. We have shown that full-length CIN85 is upregulated in podocytes in the absence of CD2AP, whereas in wild-type cells, full-length CIN85 is not detectable. In this study, we show that full-length CIN85 is postranslationally modified by SUMOylation in wild-type podocytes. We can demonstrate that CIN85 is SUMOylated by SUMO-1, -2, and -3 and that SUMOylation is enhanced in the presence of CD2AP. Conversion of lysine 598 to arginine completely abolishes SUMOylation and leads to increased binding of CIN85 to nephrin. Our results indicate a novel role for CD2AP in regulating posttranslational modification of CIN85.
Is Trauma Memory Special? Trauma Narrative Fragmentation in PTSD

Seminal theories posit that fragmented trauma memories are critical to posttraumatic stress disorder (PTSD) and that elaboration of the trauma narrative is necessary for recovery. According to fragmentation theories, trauma narrative changes, particularly for those receiving trauma-focused treatment, should accompany symptom reduction. Trauma and control narratives in 77 men and women with chronic PTSD were examined pre- and posttreatment, comparing prolonged exposure (PE) and sertraline. Utilizing self-report, rater coding, and objective coding of narrative content, fragmentation was compared across narrative types (trauma, negative, positive) by treatment modality and response, controlling for potential confounds. Although sensory components increased with PE (d = 0.23–0.44), there were no consistent differences in fragmentation from pre- to posttreatment between PE and sertraline or treatment responders and nonresponders. Contrary to theories, changes in fragmentation may not be a crucial mechanism underlying PTSD therapeutic recovery.
A Switch of G Protein-Coupled Receptor Binding Preference from Phosphoinositide 3-Kinase (PI3K)–p85 to Filamin A Negatively Controls the PI3K Pathway

ABSTRACT Frequent oncogenic alterations occur in the phosphoinositide 3-kinase (PI3K) pathway, urging identification of novel negative controls. We previously reported an original mechanism for restraining PI3K activity, controlled by the somatostatin G protein-coupled receptor (GPCR) sst2 and involving a ligand-regulated interaction between sst2 with the PI3K regulatory p85 subunit. We here identify the scaffolding protein filamin A (FLNA) as a critical player regulating the dynamic of this complex. A preexisting sst2-p85 complex, which was shown to account for a significant basal PI3K activity in the absence of ligand, is disrupted upon sst2 activation. FLNA was here identified as a competitor of p85 for direct binding to two juxtaposed sites on sst2. Switching of GPCR binding preference from p85 toward FLNA is determined by changes in the tyrosine phosphorylation of p85- and FLNA-binding sites on sst2 upon activation. It results in the disruption of the sst2-p85 complex and the subsequent inhibition of PI3K. Knocking down FLNA expression, or abrogating FLNA recruitment to sst2, reversed the inhibition of PI3K and of tumor growth induced by sst2. Importantly, we report that this FLNA inhibitory control on PI3K can be generalized to another GPCR, the mu opioid receptor, thereby providing an unprecedented mechanism underlying GPCR-negative control on PI3K.
Analgesic and Antiinflammatory Effects of the Tannin Fraction from Myracrodruon urundeuva Fr. All.

The present work showed a significant antinociceptive activity in the tannin fraction (TF) extracted from the bark of Myracrodruon urundeuva Fr. All. This inhibitory effect was demonstrated not only against abdominal contractions but also in the formalin test in mice. In the first case, at doses of 0.1 and 1 mg/kg, i.p. the TF caused inhibitions of the order of 39.6% and 80.8%, respectively, and in the second one, inhibitions of 47.8% and 77.2% (phase I) and 59.2% and 100% (phase II), after the administration of 5 and 10 mg/kg, i.p. The antinociceptive effect was partially reverted by naloxone. The TF presented also an antioedematogenic effect in rat paw oedema induced by carrageenan as well as dextran. In the carrageenan model, significant inhibitions were seen at 2 h (29.7% and 41.7%) and 3 h (40.5% and 44.2%), after administration of 5 and 10 mg/kg, i.p. In the dextran induced oedema, the TF (10 mg/kg, p.o.) caused inhibitions of 29.2%, 42.4% and 54.5% at 2 h, 3 h and 4 h, respectively. The TF (10 and 25 mg/kg, i.p.) significantly inhibited the inflammatory events (vesical oedema and increased vascular permeability) which occur at the onset of the haemorrhagic cystitis induced by cyclophosphamide. After subcutaneous or oral administration, the TF (5–50 mg/kg) also blocked neutrophil migration induced by direct (fMLP) as well as indirect (carrageenan) stimuli. © 1997 by John Wiley & Sons, Ltd.
The Laforin-Malin Complex Negatively Regulates Glycogen Synthesis by Modulating Cellular Glucose Uptake via Glucose Transporters

ABSTRACT Lafora disease (LD), an inherited and fatal neurodegenerative disorder, is characterized by increased cellular glycogen content and the formation of abnormally branched glycogen inclusions, called Lafora bodies, in the affected tissues, including neurons. Therefore, laforin phosphatase and malin ubiquitin E3 ligase, the two proteins that are defective in LD, are thought to regulate glycogen synthesis through an unknown mechanism, the defects in which are likely to underlie some of the symptoms of LD. We show here that laforin's subcellular localization is dependent on the cellular glycogen content and that the stability of laforin is determined by the cellular ATP level, the activity of 5′-AMP-activated protein kinase, and the affinity of malin toward laforin. By using cell and animal models, we further show that the laforin-malin complex regulates cellular glucose uptake by modulating the subcellular localization of glucose transporters; loss of malin or laforin resulted in an increased abundance of glucose transporters in the plasma membrane and therefore excessive glucose uptake. Loss of laforin or malin, however, did not affect glycogen catabolism. Thus, the excessive cellular glucose level appears to be the primary trigger for the abnormally higher levels of cellular glycogen seen in LD.
The Relationship between Acute Functional Status and Long-Term Ambulation after Severe Traumatic Brain Injury

Objective. To correlate long-term physical impairments of patients with severe traumatic brain injury (sTBI) based on their functional status in an acute care setting. Methods. 46 patients with sTBI participated in this prospective study. The Extended Glasgow Outcome Scale (GOSE) and the FIM instrument were rated at discharge from the acute care setting and at followup. The Functional Ambulation Classification (FAC), the Five-Meter Gait Speed, a quantified measure of negotiating stairs (Stair Climbing Speed and Rails used), and the functional reach test were rated at followup. Results. The subject with a score of 6 on the GOSE at discharge remained nonfunctional ambulator at followup. None of the subjects with a GOSE score of 5 became independent ambulators. Fifty percent of the subjects with a GOSE score of 4 were dependent ambulators. 100% of the subjects with a GOSE score of 2 or 3 at discharge were independent ambulators. A higher FIM score at discharge was associated with a greater chance of ambulating independently at 2 to 5 years after TBI (𝜒KW22𝑑𝑓). Conclusions. These data will allow physical health professionals in acute rehabilitation settings to provide more precise long-term physical outcome information to patients and families.