Miracle-dz/UniFAR-FTUs huggingface dataset:
Dissection of the Transcriptional Program Regulating Secondary Wall Biosynthesis during Wood Formation in Poplar1[C][W][OA]

Wood biomass is mainly made of secondary cell walls; hence, elucidation of the molecular mechanisms underlying the transcriptional regulation of secondary wall biosynthesis during wood formation will be instrumental to design strategies for genetic improvement of wood biomass. Here, we provide direct evidence demonstrating that the poplar (Populus trichocarpa) wood-associated NAC domain transcription factors (PtrWNDs) are master switches activating a suite of downstream transcription factors, and together, they are involved in the coordinated regulation of secondary wall biosynthesis during wood formation. We show that transgenic poplar plants with dominant repression of PtrWNDs functions exhibit a drastic reduction in secondary wall thickening in woody cells, and those with PtrWND overexpression result in ectopic deposition of secondary walls. Analysis of PtrWND2B overexpressors revealed up-regulation of the expression of a number of wood-associated transcription factors, the promoters of which were also activated by PtrWND6B and the Eucalyptus EgWND1. Transactivation analysis and electrophoretic mobility shift assay demonstrated that PtrWNDs and EgWND1 activated gene expression through direct binding to the secondary wall NAC-binding elements, which are present in the promoters of several wood-associated transcription factors and a number of genes involved in secondary wall biosynthesis and modification. The WND-regulated transcription factors PtrNAC150, PtrNAC156, PtrNAC157, PtrMYB18, PtrMYB74, PtrMYB75, PtrMYB121, PtrMYB128, PtrZF1, and PtrGATA8 were able to activate the promoter activities of the biosynthetic genes for all three major wood components. Our study has uncovered that the WND master switches together with a battery of their downstream transcription factors form a transcriptional network controlling secondary wall biosynthesis during wood formation.
Functional and clinical consequences of Fc receptor polymorphic and copy number variants

Receptors for immunoglobulins (Fc receptors) play a central role during an immune response, as they mediate the specific recognition of antigens of almost infinite diversity by leucocytes, thereby linking the humoral and cellular components of immunity. Indeed, engagement of Fc receptors by immunoglobulins initiates a range of immunoregulatory processes that might also play a role in disease pathogenesis. In the circulation, five main types of immunoglobulins (Ig) exist – namely IgG, IgA, IgE, IgM and IgD and receptors with the ability to recognize and bind to IgG (Fcγ receptor family), IgE (FcεRI and CD23), IgA (CD89; Fcα/µR) and IgM (Fcα/µR) have been identified and characterized. However, it is astonishing that nearly all the known human Fc receptors display extensive genetic variation with clear implications for their function, thus representing a substantial genetic risk factor for the pathogenesis of a range of chronic inflammatory disorders.
Immunization delivered by lentiviral vectors for cancer and infectious diseases

Summary: The increasing level of understanding of the lentivirus biology has been instrumental in shaping the design strategy of creating therapeutic lentiviral delivery vectors. As a result, lentiviral vectors have become one of the most powerful gene transfer vehicles. They are widely used for therapeutic purposes as well as in studies of basic biology, due to their unique characteristics. Lentiviral vectors have been successfully employed to mediate durable and efficient antigen expression and presentation in dendritic cells both in vitro and in vivo, leading to the activation of cellular immunity and humoral responses. This capability makes the lentiviral vector an ideal choice for immunizations that target a wide range of cancers and infectious diseases. Further advances into optimizing the vector system and understanding the relationship between the immune system and diseases pathogenesis will only augment the potential benefits and utility of lentiviral vaccines for human health.
Fully Coupled Nonequilibrium Electron–Phonon Transport in Nanometer-Scale Silicon FETs

Heat conduction from transistors and interconnects is a critical design consideration for computing below the 20-nm milestone. This paper reviews detailed heat generation and transport mechanisms in silicon devices with a focus on the nonequilibrium behavior of electrons and phonons. Fully coupled and self-consistent ballistic phonon and electron simulations are developed in order to examine the departure from equilibrium within the phonon system and its relevance for properly simulating the electrical behavior of devices. We illustrate the manner in which nanoscale-transport phenomena are critically important for a broad variety of low-dimensional silicon-based devices, including FinFETs and depleted substrate transistors.
Long‐term outcome of intravenous therapy with rituximab in patients with primary cutaneous B‐cell lymphomas

The monoclonal antibody rituximab directed against the B‐cell antigen CD20 was approved for the treatment of B‐cell lymphomas and maintenance therapy in follicular lymphomas more than a decade ago. However, median follow‐up in case series of intravenous rituximab therapy in primary cutaneous B‐cell lymphomas (CBCL) lasts only up to 3 years. We retrospectively analysed a cohort of CBCL patients treated with rituximab to gain more long term information.
Omega-conotoxin sensitivity and presynaptic inhibition of glutamatergic sensory neurotransmission in vitro

Synaptic transmission between embryonic chick dorsal root ganglion (DRG) neurons and spinal cord neurons was studied in dissociated cell culture. Stimulation of DRG neurons evoked monosynaptic and polysynaptic excitatory responses in the spinal neurons. These responses could be reversibly blocked by application of 6-cyano-7- nitroquinoxaline-2,3-dione (a selective non-NMDA receptor antagonist) and irreversibly eliminated through the presynaptic action of omega- conotoxin GVIA (a selective N-type calcium channel antagonist). As N- type calcium channels in DRG neuron somata are targets for modulation via GABAB receptors, we tested the role of these receptors as regulators of synaptic transmission. Baclofen (a selective GABAB receptor agonist) reversibly inhibited synaptic transmission via a presynaptic, pertussis toxin-sensitive mechanism; CGP 35348 (a selective GABAB receptor antagonist) blocked the actions of baclofen. Taken together, these results demonstrate that N-type calcium channels play a dominant role in glutamatergic sensory neurotransmission. They suggest, in addition, that modulation of N-channel activity may underlie, at least in part, presynaptic inhibition of synaptic transmission between DRG neurons and their targets in the intact spinal cord.
Incidence of intracranial meningiomas in Denmark, Finland, Norway and Sweden, 1968–1997

It has been reported that the incidence of meningioma increased in several industrialized countries in the late 1970s and early 1980s. The aim of this study was to evaluate the time trends in incidence of meningiomas in Denmark, Finland, Norway and Sweden, with emphasis on the age distribution and sex ratio. Information about cases of meningiomas in people aged 15–84 years was obtained from the cancer registries of these Nordic countries for the years 1968–1997, and estimates of person‐years at risk were calculated from information provided by the national population registries. Age‐specific incidence rates per 100,000 and incidence rate ratios were calculated for 3‐year periods. The female:male ratios were also evaluated. The combined incidence among men increased from 1.4 to 1.9 per 100,000 during the follow‐up period, the corresponding rates for women were 2.6 and 4.5. The female:male ratio increased over time for several age groups and was as high as 3.5:1 in the group aged 40–44 years in the latest follow‐up period (1993–1997). In summary, our results provide some support for the idea that the introduction of computed tomography in the late 1970s has had an impact on the detection of cases in people aged 60 and over. The decrease in the rate or detection postmortem has affected the incidence time trend, but it also coincides with widespread use of new imaging technologies. The increasing trend shown for the female:male ratio in the group aged 35–59 years is consistent with the possibility that increasing use of hormones may affect the incidence of meningiomas in women. © 2005 Wiley‐Liss, Inc.
Successful treatment of psoriasis with low‐dose per os interleukins 4, 10, and 11

Roberti et al. (1) have recently reported on the successful immunomodulating treatment with simultaneously administered low-dose interleukins (ILs)-4, -10, and -11 in the treatment of psoriasis vulgaris. This treatment is an example of therapies based on the Physiological Regulation Medicine concepts. Physiological Regulation Medicine represents the most up-to-date integration of low-dose medicine with the innovative therapeutic concept of restoring physiology through activated low dose of communicating molecules such as hormones, neuropeptides, interleukins, and growth factors. The low-dose preparation method, called sequential kinetic activation (SKA), seems to make communicating molecules more effective, providing a biofeedback mechanism capable of restoring the psyco-neuro-immuno-endocrine homeostatic balance (2–5). This concept represents the “functional core” of low-dose medicine. Thanks to SKA technology (the “technical core” of low-dose medicine), it is now possible to use lower doses of communicating molecules (in the range of pg/mL) with therapeutic results that are apparently comparable with those induced by high concentrations but with reduced side effects as demonstrated by both preclinical and clinical studies (1,5). This innovative approach enables the provision of synergic, effective, and safe molecules that are the cornerstone of low-dose medicine. The authors (1) have administered simultaneously per os 10 fg/mL IL-10, IL-11 and IL-4 to 48 psoriatic subjects affected by moderate recalcitrant plaque psoriasis, in multicenter, double-blind, randomized, placebo-controlled clinical trial according to a crossover model using either the cytokines treatment or placebo, alternatively. Patients have been followed up for 8 months. Forty-one out of 48 subjects have completed the study. In the 41 treated patients PASI significantly decreased (Friedman test: p = 0.00960) and in parallel DLQI significantly improved (Friedman test: p = 0.00007). The efficacy and safety of the treatment with low-dose cytokines (IL-10, IL-11, and IL-4) simultaneously administered per os was shown. No adverse effect was reported in any of the psoriatic patients treated. The availability of low-dose remedies containing diluted and SKA-activated cytokines represents a unique opportunity for an innovative immunological therapeutic approach for psoriasis and other dermatological immune-mediated diseases.
Quantitative Assessment of &bgr;-Cell Apoptosis and Cell Composition of Isolated, Undisrupted Human Islets by Laser Scanning Cytometry

Background. Assays for assessing human islet cell quality, which provide results before transplantation, would be beneficial to improve the outcomes for islet transplantation therapy. Parameters such as percent &bgr;-cell apoptosis and cell composition are found to vary markedly between different islet preparations and may serve as markers of islet quality. We have developed fluorescence-based assays using laser scanning cytometry for assessing &bgr;-cell apoptosis and islet cell composition on serial sections of intact isolated islets. Methods. Isolated human islets were fixed in formalin and embedded in paraffin. Serial sections were immunostained for the pancreatic hormones and acinar and ductal cell markers. DNA fragmentation was used to label apoptotic cells. Stained cells were quantified using an iCys laser scanning cytometer. Results. Islet preparations from 102 human pancreatic islet isolations were analyzed. For the whole set of islet preparations, we found a mean islet cell composition of 54.5%±1.2% insulin-positive, 33.9%±1.2% glucagon, 12.1%±0.7% somatostatin, and 1.5%±0.2% pancreatic polypeptide-positive cells. The apoptotic &bgr; cells were 2.85%±0.4% with a range of 0.27% to 18.3%. The percentage of apoptotic &bgr; cells correlated well (P<0.0001, n=59) with results obtained in vivo by transplantation of the corresponding islets in diabetic NODscid mice. Conclusions. The analysis of whole, nondissociated islets for cell composition and &bgr;-cell apoptosis using laser scanning cytometry gives reliable and reproducible results and could be performed both before islet transplantation and on preserved cell blocks at any time in future. Thus, they can be a powerful tool for islet quality assessment.
Electrophoretic analysis of seed storage proteins from gymnosperms

Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), pore gradient gel electrophoresis (PGGE) followed by SDS‐PAGE and Western blot analysis were used to characterize the seed storage proteins from seven gymnosperm species from the families Pinaceae (Abies alba, Cedrus atlantica and Picea abies), Cupressaceae (Biota orientalis, Chamaecyparis lawsoniana and Cupressus arizonica), and Taxaceae (Taxus baccata). SDS‐PAGE and PGGE X SDS‐PAGE indicate the presence of proteins with characteristics similar to the 7S globulins in all the species studied. Antibodies to a 7S globulin subunit from Pinus pinaster cross‐reacted with homologous polypeptides from Pinaceae species, but not with corresponding subunits from species belonging to other families. Also detected in each of the studied species, with the exception of A. alba and T. baccata, were proteins potentially homologous with 11S proteins. Of these proteins, only those of C. atlantica may be extracted by saline buffer, while the remainder require a dissociating agent. Antibodies raised against the small subunit from P. pinaster 11S protein recognized only the corresponding polypeptides from Pinaceae species. Overall, these results help clarify our knowledge of gymnosperm seed storage proteins.
The effects of self-threats and affirmations on romantic relationship functioning: The moderating roles of self-esteem and relationship-contingent self-esteem

Although romantic relationships are an important source of self-esteem, individuals vary in the degree to which romantic relationships determine their self-esteem. For individuals with relationship-contingent self-esteem (RCSE), self-esteem is based on the quality of perceived romantic relationship functioning. In contrast, global selfesteem is derived from a variety of domains, not specifically relationship quality. The present study investigated the moderating effects of RCSE and global self-esteem on the effects of relationship specific or relationship-unrelated threats and selfaffirmations. Individuals with low global self-esteem react to threats by distancing themselves from their romantic partners. For those low in RCSE, this should occur only when the threats are relationship specific, whereas those low in global self-esteem distance themselves regardless of what type of threat they experience. Exposure to self-affirmations limits this defensive distancing in individuals with low global selfesteem. Prior studies examining the effects of induced self-threats and self-affirmations on perceived relationship functioning have been limited by reliance on college student samples, whose relationships are often shorter in duration than older adults. The current studies examined self-esteem and RCSE within the context of older participants in longer romantic relationships. Specifically, Study 1 examined how self-threats and self-affirmations interacted with dispositional levels of RCSE and self-esteem to predict romantic relationship outcomes. Participants wrote about past experiences to induce self-affirmations or self-threats, which were either relational (i.e., about their current relationship) or non-relational (i.e., about their personal lives), and then completed measures of relationship functioning. Contrary to predictions, there were no significant interactions between either RCSE or self-esteem and the experimental conditions. There was a significant main effect for self-esteem, such that participants with low self-esteem reported less commitment, closeness, and satisfaction as compared to participants with high self-esteem. In Study 2, participants completed one of two writing prompts: a prompt to induce high RCSE, or a control prompt. Participants then wrote about past experiences to induce selfthreats that were either relational or non-relational. Contrary to hypotheses, inducing high levels of RCSE and exposing participants to a relational self-threat did not result in decreased relationship commitment, closeness, or satisfaction. The results of both Study 1 and Study 2 are contrary to previous research; potential explanations for this discrepancy and implications are discussed.
Increased risk of colorectal neoplasia in patients with primary sclerosing cholangitis and ulcerative colitis: a meta-analysis.

BACKGROUND Published data on the risk of colorectal neoplasia in patients with ulcerative colitis with and without primary sclerosing cholangitis are conflicting. A meta-analysis was performed to synthesize available publications and to compare the risk of colorectal neoplasia in patients with ulcerative colitis with and without primary sclerosing cholangitis. METHODS By using MEDLINE and manual search methods, studies were identified that compared the risk of colorectal neoplasia (dysplasia and carcinoma) in patients with ulcerative colitis with and without primary sclerosing cholangitis. In addition, citations were reviewed in relevant articles and proceedings from gastroenterology meetings, and investigators were contacted when data were incomplete. The summary odds ratio (OR) was then calculated for the risk for patients with ulcerative colitis and primary sclerosing cholangitis of having colorectal neoplasia develop compared with that of patients with ulcerative colitis without primary sclerosing cholangitis. RESULTS Eleven studies met all eligibility criteria for the meta-analysis. Patients with ulcerative colitis and primary sclerosing cholangitis are at increased risk of colorectal dysplasia and carcinoma compared with patients with ulcerative colitis alone; OR 4.79: 95% CI [3.58, 6.41] with the Mantel-Haenszel method, and OR 5.11: 95% CI [3.15, 8.29] with the Der Simonian and Laird method. This increased risk is present even when the risk of colorectal carcinoma alone is considered; OR 4.09: 95% CI [2.89, 5.76] and OR 4.26: 95% CI [2.80, 6.48] by using, respectively, the Mantel-Haenszel and the Der Simonian and Laird methods. CONCLUSIONS Patients with ulcerative colitis and primary sclerosing cholangitis have a significantly higher risk for the development of colorectal neoplasia than patients with ulcerative colitis but not primary sclerosing cholangitis. More intensive colonoscopic surveillance should be considered for patients with ulcerative colitis and primary sclerosing cholangitis.
[Experimental study on effect of Buchang Naoxintong containing serum for antagonizing hypoxia-induced apoptosis of cortical neurons].

OBJECTIVE To investigate the effect of Buchang Naoxintong containing serum (BNCS) for antagonizing hypoxia-induced apoptosis of primary cultured cortical neurons with the sero-pharmacological method. METHODS Primary cortical neurons from neonate rats (within 24 h) were cultured and induced into hypoxia model on the 7th day, which were then treated with different concentrations of BNCS. Cell apoptosis was detected qualitatively and quantitatively; and further verified by agarose gel electrophoresis through analyzing in-ternucleosomal DNA fragmentation of the neurons. Besides, neuron viability was tested by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and change of nuclear morphology was observed by Hoechst 33258 staining. RESULTS After treatment with BNCS, the viability of the hypoxia neurons improved with significantly reduced neuron apoptosis. CONCLUSION Buchang Naoxintong can protect cortical neurons from hypoxia-induced apoptosis.
The Structural Burden of Caregiving: Shared Challenges in the United States and Canada

In contrasting health care structures, we each served as caregivers to elderly parents where a shared and unexpected theme in our experiences was the substantial burden of negotiating and managing long-term care (LTC) services within our respective health and social care systems. In this article, we introduce and elucidate an under recognized source of caregiver burden in the United States and Canada: the structural burden of caregiving. We draw on shared and unique experiences cross-nationally, along with the literature, to illustrate that (a) today’s caregiving is increasingly characterized by interactions with formal health and social systems in negotiating and managing services, (b) these systems are hampered by discontinuous and fragmented care which increase caregiver stress, and (c) this structural burden likely exacerbates inequity for both care recipients and caregivers. In conclusion, we call for theoretical models of caregiving to highlight health and social systems as creating burden and for measurement of caregiver burden to explicitly consider the time and stress stemming from interactions with formal health and social systems. Finally, we call for future policy evaluation to incorporate structural burden as an additional outcome in considering changes to LTC provisions and funding.
Comparison of standard and task-specific enhancement of Digora storage phosphor images for approximal caries diagnosis.

OBJECTIVES To compare approximal caries detection on Digora storage phosphor images pre-enhanced with the automatic "caries-specific Oslo enhancement procedure" ("Oslo-enhanced" method) and storage phosphor images individually enhanced by observers particularly experienced in digital imaging using standard brightness and contrast functions of the Digora system ("Digora-enhanced" method). METHODS Seven staff members from four oral radiology departments rated 240 approximal surfaces for caries with regard to lesion depth in the inner and outer half of the enamel and dentine, using a 5-point confidence scale. The observations were validated histologically. A receiver operating characteristic (ROC) analysis and an analysis of variance with three dependent variables (observer confidence, observer signed error and observer absolute error) were performed. RESULTS The most evident difference between the methods as elucidated by the ROC analysis was the highly significant smaller interobserver and intraobserver variance with the Oslo-enhanced method for all but one observer. The methods were not different with regard to average diagnostic accuracy (A(z) values) as tested with paired t-tests, and there was no correlation between A(z) across methods. The method and lesion main effects, as well as the lesion by method interaction effect, were multivariately significant (P<0.001) in favour of the Oslo-enhanced method. On a univariate level, the method main effect was not significant for the absolute observer error (P=0.330). All other univariate effects were significant (P<0.001). CONCLUSIONS The accuracy of approximal caries detection with Digora storage phosphor images pre-enhanced and images individually enhanced was similar, but interobserver and intraobserver variability improved with the Oslo-enhanced method. Since image manipulation is not performed with the Oslo-enhanced method, the increased speed of the diagnostic procedure combined with the improved observer variability would probably be even more pronounced for the general dental practitioner.