Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluating factors that affect the shade-matching ability of dentists, dental staff members and laypeople.

BACKGROUND The authors conducted a study to evaluate the influence of dentists' and nondentists' experience, age, sex, eye color and use of eyeglasses or contact lenses on tooth shade-matching ability. METHODS The authors included 120 participants in this study conducted in Istanbul (periodontists, oral and maxillofacial surgeons, orthodontists, endodontists, pediatric dentists, prosthodontists, restorative dentists, general dentists in private practice, dental technicians, dental assistants, dental assistant students and laypeople). The authors assigned participants to one of three groups: group 1 was composed of prosthodontists, restorative dentists and dental technicians; group 2 consisted of other dental specialists and general dentists; and group 3 included dental assistants, dental assistant students and laypeople. The authors asked participants to match the shades of three artificial maxillary right central incisors (Vitapan acrylic teeth [shades 2L1.5, 1M2, 2R1.5], Vita Zahnfabrik, Bad Säckingen, Germany) by using a shade guide system (Vita Toothguide 3D-Master, Vita Zahnfabrik). They calculated shade matching for the three color components (value, hue, chroma) and analyzed the results by using a chi(2) test. RESULTS The rate of success in matching the shade for IM2 was 53.3 percent for participants in group 1, 30 percent for participants in group 2 and 20 percent for participants in group 3 (P = .017). However, there were no significant differences between the three groups for shades 2L1.5 and 2R1.5. Professional experience (P = .003) and age (P = .027) were associated with shade-matching success for tooth shade 2L1.5 only. The results showed no statistically significant differences with respect to sex, eye color or use of eyeglasses or contact lenses. CONCLUSIONS Dental care professionals who routinely performed restorative procedures matched the shades better than did participants in other groups. Professional experience was associated positively with the outcome, while sex, eye color and use of eyeglasses or contact lenses did not have any effect on shade-matching results. CLINICAL IMPLICATIONS To improve shade-matching skills, clinicians should participate in hands-on courses, continuing education classes and other training programs.
Inconsistent Immunohistochemical Expression Patterns of Four Different CD133 Antibody Clones in Glioblastoma

The putative tumor stem cell marker CD133 is the marker of choice for identifying brain tumor stem cells in gliomas, but the use of different CD133 antibody clones possibly recognizing different CD133 splice variants with epitopes of different glycosylation status confuses the field. The aim was to investigate if current inconsistent CD133 observations could be a result of using different CD133 antibodies for immunohistochemical identification of CD133. Ten glioblastomas were immunohistochemically stained with four different CD133 antibody clones (AC133, W6B3C1, C24B9, and ab19898) and analyzed by quantitative stereology. Moreover, the CD133 staining pattern of each antibody clone was investigated in kidney, pancreas, and placenta tissue as well as in glioblastoma and retinoblastoma cultures and cell lines. All antibody clones revealed CD133+ niches and single cells in glioblastomas, but when using different clones, their distribution rarely corresponded. Morphology of identified single cells varied, and staining of various tissues, cultures, and cells lines was also inconsistent among the clones. In conclusion, the authors report inconsistent CD133 detection when using different primary CD133 antibody clones. Thus, direct comparison of studies using different antibody clones and conclusions based on CD133 immunohistochemistry should be performed with caution.
Nestin is a novel marker for renal tubulointerstitial injury in immunoglobulin A nephropathy

Aim: Nestin, an intermediate filament originally identified as a marker of neural progenitor cells, is transiently expressed in endothelial cells and tubuloepithelial cells during kidney development. However, in adult kidneys, podocytes are the only cells that express nestin. In this study, we examined tubulointerstitial nestin expression in human glomerulonephritis.
Melatonin regulates proteomic changes during leaf senescence in Malus hupehensis

Despite the relationship between melatonin and aging, the overall changes and regulation of proteome profiling by long‐term melatonin exposure during leaf senescence is not well understood. In this study, leaf senescence in Malus hupehensis plants was delayed when exogenous melatonin was regularly applied to the roots for 2 months compared with natural leaf senescence. Proteins of samples 0 and 50 day for both treatments were extracted and labeled with TMT regents before being examined via NanoLC‐MS/MS. The proteomics data showed that 622 and 309 proteins were altered by senescence and melatonin, respectively. Our GO analysis by Blast2GO revealed that most of the altered proteins that are involved in major metabolic processes exhibited hydrolase activity and were mainly located in the plastids. These proteins were classified into several senescence‐related functional categories, including degradation of macromolecules, redox and stress responses, transport, photosynthesis, development, and other regulatory proteins. We found that melatonin treatment led to the downregulation of proteins that are normally upregulated during senescence. The melatonin‐related delay in senescence might have occurred due to the altering of proteins involved in processes associated with senescence. And as well, there are many unknown regulatory proteins possibly being involved in the melatonin's function. This study is the first to demonstrate changes at the proteome level in response to exogenous melatonin in plants. Our findings provide a set of informative and fundamental data about the role of melatonin in apple leaf senescence.
Epidemiology of double aneuploidies involving chromosome 21 and the sex chromosomes

The chance of two chromosome abnormalities occurring in one conceptus is very small. However, some authors have suggested that double aneuplodies (DAs) might be more common than the product of their individual frequencies. The nonrandomness of such DA events was considered to be evidence that nondisjunction (NDJ) may be genetically determined. Data collected from the National Down syndrome Cytogenetic Register (NDSCR) in England and Wales and from the literature indicate that the frequencies of all nonmosaic DAs, except for 48,XXY,+21, are lower than expected, probably because of strong intrauterine selection against such pregnancies. Collectively, we identified 52 cases of nonmosaic 48,XXY,+21; 28 cases of 48,XYY,+21; and 14 cases of 48,XXX,+21 in liveborns and 13 cases of 48,XXY,+21; four cases of 48,XYY,+21; and two cases of 48,XXX,+21 after prenatal diagnoses. Among these cases, analysis of the published unbiased cytogenetic surveys of liveborn DS revealed 24 cases of 48,XXY,+21; nine cases of 48,XYY,+21; and seven cases of 48,XXX,+21. These figures are different from the expected proportion of 1:1:1 (P < 0.001), with carriers of XXY overrepresented in the group of carriers of DA. Mechanisms put forth to account for the higher occurrence of 48,XXY,+21 may include greater accessibility of disomic ovum to Y‐carrying sperm, and promotion of NDJ in ovum by Y‐bearing sperm. 48,XXY,+21 DA was found to be age‐dependent, as the proportion of mothers over age 35 (x = 33.0) was increased over the general population. This is in contrast to the apparently age‐independent 48,XYY,+21 DA, with a mean maternal age of 24.7 (P < 0.001). Paternal ages were also remarkably different between the groups, with a mean age of 37.9 in 48,XXY,+21 cases and a mean age of 27.9 in 48,XYY,+21 cases (P < 0.01). Maternal age‐related factors, rather than genetic predisposition, may play a more important role in the etiology of the most common DA, 48,XXY,+21. © 2005 Wiley‐Liss, Inc.
Psychometric parameters of the Persian version of the BriefCOPE among wives of patients under hemodialysis

Background: The Brief COPE is widely validated and popularly used in assessing coping strategies in major life stressors on various populations. This study carried out to determine the validity and reliability of the Persian version of the Brief COPE among wives of patients under hemodialysis. Methods: The Brief COPE was translated into Persian language according to the standard method and the psychometric properties of the instrument were assessed among 212 wives of patients under hemodialysis in Tehran, Iran, in 2016. Content validity was established, by 15 expert opinions. Face validity was determined by respondents and expert opinion. Construct validity was analyzed through confirmatory factor analysis. The Cronbach's alpha coefficient and intraclass correlation coefficient were used to determine the internal consistency and test-retest reliability, respectively. Results: All 28 Items of Brief COPE scale had content validity index greater than 0.7, suggesting a good validity value of the items in terms of relevancy (range 0.8 - 0.94). The wording of some items was modified to make them more readily understood by the participants, although the core meaning of each item was kept intact and to observe Iranian cultural adaptation; two items were adjusted. Result of confirmatory factor support 14 factor structure of the scale has good fit. Cronbach's alpha coefficient was acceptable for the total scale (α= 0.77) and for subscales (range 0.7 - 0.91). The intraclass correlation coefficient was acceptable for scale (r=0.76, P value= 0.001). Conclusion: The Persian translation of the Brief COPE is a valid and reliable instrument to determine coping strategies in women living with the husband on hemodialysis.
Declining transition rates to psychosis: the contribution of potential changes in referral pathways to an ultra–high‐risk service

On the basis of applying ‘ultra–high‐risk’ (UHR) criteria, initially high rates of transition to psychosis were reported. However, a decline in transition to psychosis has been observed in recent years. The current descriptive paper aims to investigate if this drop in transition rate may be due to potential changes in patterns of referral to a large UHR clinic.
AlGaInP microcavity light-emitting diodes at 650 nm on Ge substrates

We demonstrate microcavity light emitting diodes (MCLEDs) emitting at 650 nm on Ge substrates. Ge has the advantage of lower cost and higher strength compared to GaAs substrates. The multi-quantum well microcavity devices consisted of AlGaAs-based distributed Bragg reflector (DBR) mirrors, AlGaInP active material with an additional 5-/spl mu/m p-Al/sub 0.55/Ga/sub 0.45/As current spreading layer on top of the p-DBR. A maximum external quantum efficiency of 4.35% and an optical power higher than 5 mW was obtained for a device with 200-/spl mu/m diameter. The results indicate the potential use of MCLEDs on Ge for visible LEDs.
High‐grading bias: subtle problems with assessing power of selected subsets of loci for population assignment

Recognition of the importance of cross‐validation (‘any technique or instance of assessing how the results of a statistical analysis will generalize to an independent dataset’; Wiktionary, en.wiktionary.org) is one reason that the U.S. Securities and Exchange Commission requires all investment products to carry some variation of the disclaimer, ‘Past performance is no guarantee of future results. ’ Even a cursory examination of financial behaviour, however, demonstrates that this warning is regularly ignored, even by those who understand what an independent dataset is. In the natural sciences, an analogue to predicting future returns for an investment strategy is predicting power of a particular algorithm to perform with new data. Once again, the key to developing an unbiased assessment of future performance is through testing with independent data—that is, data that were in no way involved in developing the method in the first place. A ‘gold‐standard’ approach to cross‐validation is to divide the data into two parts, one used to develop the algorithm, the other used to test its performance. Because this approach substantially reduces the sample size that can be used in constructing the algorithm, researchers often try other variations of cross‐validation to accomplish the same ends. As illustrated by Anderson in this issue of Molecular Ecology Resources, however, not all attempts at cross‐validation produce the desired result. Anderson used simulated data to evaluate performance of several software programs designed to identify subsets of loci that can be effective for assigning individuals to population of origin based on multilocus genetic data. Such programs are likely to become increasingly popular as researchers seek ways to streamline routine analyses by focusing on small sets of loci that contain most of the desired signal. Anderson found that although some of the programs made an attempt at cross‐validation, all failed to meet the ‘gold standard’ of using truly independent data and therefore produced overly optimistic assessments of power of the selected set of loci—a phenomenon known as ‘high grading bias. ’
Development of Outer Hair Cells in Ames Waltzer Mice: Mutation in Protocadherin 15 Affects Development of Cuticular Plate and Associated Structures

The Ames waltzer (av) mouse mutant harbors a mutation in the protocadherin 15 gene (Pcdh15) and is a model for deafness in Usher syndrome 1F and nonsyndromic deafness DFNB23. Mutation in Pcdh15 affects stereocilia morphogenesis and polarity. Disruptions of apical cellular components in outer hair cells have also been described in av mutants. Organization of stereocilia and cell polarization may be dependent on proper orientation of structural components residing in the apical portion of the cell during development. We used electron and immunofluorescent microscopy to examine structural maturation of outer hair cells in av3J mice with emphasis on the fonticulus, basal body/centriole complex, actin mesh, and the microtubule network during initiation of bundle organization, between embryonic day (E) 16.5 and postnatal day 5 (P5). We found major ultrastructural rearrangements near the hair cell surface in av3J mice. Earliest changes were in kinocilia, basal body, and stereocilia positioning and microtubule arrangement once the kinocilia had lateralized to the side of the cell (between E16.5 and postnatal day [P] 0, before cuticular plate formation and stereocilia elongation). By P0, the developing fonticulus in av mice appeared enlarged, with a normal vesicle density. Stereocilia bundle disorganization increased after P0, with disruptions of the actin mesh within the cuticular plate. These observations support the hypothesis that mutations in Pcdh15 in av3J mice adversely affect coordinated maturation of apical cell components, resulting in disturbed stereocilia bundle polarity in av mice. Anat Rec, 2007. © 2008 Wiley‐Liss, Inc.
The severity of duodeno‐esophageal reflux influences the development of different histological types of esophageal cancer in a rat model

The mechanism through which each histological type of carcinoma arises from the esophageal mucosa remains unknown. This study was designed to investigate whether there is an association between the severity of duodeno‐esophageal reflux and the histological type of esophageal cancer. A series of 120 male Fischer rats, weighing ∼180 g, were randomized to receive one of the following procedures: duodeno‐forestomach reflux (DFR) with reduced exposure to duodenal contents, duodeno‐esophageal reflux (DER) with increased exposure to duodenal contents and three control operations (DFR, DER control and sham). The reflux of bile was estimated with 99mTc‐PMT scintigraphy. All animals were fed a standard diet without carcinogen. The esophageal mucosa was assessed 50 weeks after surgery for carcinoma. The median scanned fraction rate of duodeno‐esophageal reflux was significantly lower for the rodents in the DFR group than those in the DER group. Five of 28 rodents in the DFR group and 17 of the 22 rodents in the DER group developed esophageal carcinoma. None of the controls developed carcinoma. The five rodents in the DFR group developed SCC. Of 22 esophageal carcinomas for the DER group, nine were SCC, 12 ADC and one was adenosquamous carcinoma. The fraction of esophageal SCC for the DFR group was significantly higher than that for the DER group, while the fraction of esophageal ADC for the DFR group was significantly lower than that for the DER group. These observations suggest that the severity of duodeno‐esophageal reflux in rodents is related to the development of different histological types of esophageal carcinoma.
Risk factors influencing mortality of patients with severe acute pancreatitis within 24 hours after admission.

OBJECTIVE To study the risk factors for death of patients with severe acute pancreatitis (SAP) within 24 hours after admission. METHODS Clinical and laboratory data of 74 patients with SAP were analyzed retrospectively. The 27 possible risk factors for death within 24 hours after admission were investigated using logistic regression (SPSS software package 10.0), and the equation of logistic regression was set up. RESULTS Among the 27 possible risk factors, arterial pH, APACHE II scores, early shock, multiple organ failure were associated with mortality. Single logistic regression analysis of the 27 parameters showed that early shock, pleural effusion, arterial pH, complications and APACHE II scores were associated with death of SAP patients, but using multiple logistic regression analysis showed that only acidosis (pH<7.35) was associated with death. CONCLUSION To maintain the function of organs and correct dysequilibrium of water-electrolyte and acid-base in early treatment of SAP is essential to lower the mortality.
Modified elliptical rotation flap for sacrococcygeal pilonidal sinus disease

Although various surgical procedures have been described for pilonidal sinus disease, the best surgical technique is still controversial. The aim of this study was to evaluate the short‐term results of modified elliptical rotation flap (MERF) for pilonidal sinus disease in terms of post‐operative complications, recurrence and quality of life.
Homo‐ and heterodimerization of ROCO kinases: LRRK2 kinase inhibition by the LRRK2 ROCO fragment

Mutations in the gene encoding leucine‐rich repeat kinase 2 (LRRK2) are the most common cause of autosomal‐dominant familial and late‐onset sporadic Parkinson’s disease (PD). LRRK2 is a large multi‐domain protein featuring a GTP‐binding C‐terminal of Ras of complex proteins (ROC) (ROCO) domain combination unique for the ROCO protein family, directly followed by a kinase domain. Dimerization is a well‐established phenomenon among protein kinases. Here, we confirm LRRK2 self‐interaction, and provide evidence for general homo‐ and heterodimerization potential among the ROCO kinase family (LRRK2, LRRK1, and death‐associated protein kinase 1). The ROCO domain was critically, though not exclusively involved in dimerization, as a LRRK2 deletion mutant lacking the ROCO domain retained dimeric properties. GTP binding did not appear to influence ROCOLRRK2 self‐interaction. Interestingly, ROCOLRRK2 fragments exerted an inhibitory effect on both wild‐type and the elevated G2019S LRRK2 autophosphorylation activity. Insertion of PD mutations into ROCOLRRK2 reduced self‐interaction and led to a reduction of LRRK2 kinase inhibition. Collectively, these results suggest a functional link between ROCO interactions and kinase activity of wild‐type and mutant LRRK2. Importantly, our finding of ROCOLRRK2 fragment‐mediated LRRK2 kinase inhibition offers a novel lead for drug design and thus might have important implications for new therapeutic avenues in PD.
Acyl‐CoA synthetase as a cancer survival factor: its inhibition enhances the efficacy of etoposide

Lipid metabolism is often elevated in cancer cells and plays an important role in their growth and malignancy. Acyl‐CoA synthetase (ACS), which converts long‐chain fatty acids to acyl‐CoA, is overexpressed in various types of cancer. However, the role of ACS in cancer remains unknown. Here, we found that ACS enzyme activity is required for cancer cell survival. Namely, the ACS inhibitor Triacsin c induced massive apoptosis in glioma cells while this cell death was completely suppressed by overexpression of ACSL5, the Triacsin c–resistant ACS isozyme, but not by overexpression of a catalytically inactive ACSL5 mutant. ACS inhibition by Triacsin c markedly potentiated the Bax‐induced intrinsic apoptotic pathway by promoting cytochrome c release and subsequent caspase activation. These effects were abrogated by ACSL5 overexpression. Correspondingly, ACS inhibition synergistically potentiated the glioma cell death induced by etoposide, a well‐known activator of apoptosis. Furthermore, in a nude mouse xenograft model, Triacsin c at a non‐toxic dose enhanced the antitumor efficacy of a low‐dose chemotherapy with etoposide. These results indicate that ACS is an apoptosis suppressor and that ACS inhibition could be a rational strategy to amplify the antitumor effect of etoposide. (Cancer Sci 2009)
Mucin Core Peptide Expression Can Help Differentiate Barrett's Esophagus From Intestinal Metaplasia of the Stomach

It is important to distinguish Barrett's esophagus (BE) from intestinal metaplasia related to carditis because these conditions have a different natural history, risk of malignancy, and treatment. However, the distinction between these entities is difficult both clinically and pathologically. The aim of this study was to evaluate and compare the immunostaining pattern of five mucin core polypeptides in BE to cases of carditis or antritis with intestinal metaplasia. Routinely processed mucosal biopsies from 22 patients with intestinal-type BE, 24 patients with cardia intestinal metaplasia (10 Helicobacter pylori positive), 17 patients with antral intestinal metaplasia (all H. pylori positive), 20 control patients with a normal antrum, and 22 control patients with a normal cardia were immunostained with monoclonal antibodies against MUC1, MUC2, MUC3, MUC5AC, and MUC6 mucin core polypeptides. Staining was evaluated separately for goblet cells and non-goblet columnar cells and compared between all groups. A significantly higher number of BE cases (P < 0.05) showed goblet cell staining for MUC1 (55%) or MUC6 (32%) compared with patients with carditis with intestinal metaplasia (MUC1 14%, MUC6 7%) or antritis with intestinal metaplasia (MUC1 6%, MUC6 0%). BE also showed a higher frequency of MUC1 and MUC6 positivity in non-goblet columnar cells compared with carditis and antritis cases with intestinal metaplasia. Only cases of BE showed combined MUC1 and MUC6 staining (sensitivity 23%, specificity 100%). The sensitivity and specificity of MUC1 staining for BE are 55% and 96%, respectively, and for MUC6 staining 30% and 96%, respectively. Interestingly, normal gastric cardia mucosa also showed a significantly higher prevalence of MUC2 and MUC3 expression in glandular epithelium (29% and 38%, respectively) compared with the antrum (0% for both markers) (P < 0.05). In conclusion, MUC1 and MUC6 expression in BE is distinct from that of the cardia and antrum with intestinal metaplasia; thus, immunophenotyping for these markers may have some value in a subset of patients in helping to separate BE from patients with intestinal metaplasia of the cardia. Columnar epithelium in the “normal” gastric cardia has a partially intestinalized phenotype and, as a result, may represent an early form of metaplastic epithelium.