Miracle-dz/UniFAR-FTUs huggingface dataset:
Discovery of aspirin-triggered eicosanoid-like mediators in a Drosophila metainflammation blood tumor model

ABSTRACT Epidemiologic studies have linked the use of aspirin to a decline in chronic inflammation that underlies many human diseases, including some cancers. Aspirin reduces the levels of cyclooxygenase-mediated pro-inflammatory prostaglandins, promotes the production of pro-resolution molecules, and triggers the production of anti-inflammatory electrophilic mono-oxygenated (EFOX) lipid mediators. We investigated the effects of aspirin in fruit fly models of chronic inflammation. Ectopic Toll/NF-κB and JAK/STAT signaling in mutant D. melanogaster results in overproliferation of hematopoietic blood progenitors resulting in the formation of granuloma-like tumors. Ectopic JAK-STAT signaling also leads to metabolic inflammation. We report that aspirin-treated mutant flies experience reduction in metabolic inflammation, mitosis, ectopic immune signaling, and macrophage infiltration. Moreover, these flies synthesize 13-HODE, and aspirin triggers 13-oxoODE (13-EFOX-L2) production. Providing the precursor of 13-HODE, linoleic acid, or performing targeted knockdown of the transcription factor STAT in inflammatory blood cells, boosts 13-EFOX-L2 levels while decreasing metabolic inflammation. Thus, hematopoietic cells regulate metabolic inflammation in flies, and their effects can be reversed by pharmaceutical or dietary intervention, suggesting deep phylogenetic conservation in the ability of animals to resolve inflammation and repair tissue damage. These findings can help identify novel treatment targets in humans. Summary: Aspirin administration shrinks blood tumors and resolves inflammation in fruit flies. These effects correlate with high levels of linoleic acid-derived bioactive lipids.
Perceived quality of instruction: The relationship among indicators of students' basic needs, mastery goals, and academic achievement.

BACKGROUND Students' mastery goals are positively related to adaptive learning behaviour. Moreover, these goals often mediate the relation between perceived classroom characteristics and academic achievement. Research generally shows a decline of academic achievement and mastery goals after transition to middle school. Creating a learning environment at middle school according to students' basic needs for autonomy, competence, and social relatedness might help to reduce these declines. However, little is known about the relationship between perceived fulfilment of needs, mastery goals, and academic achievement. AIMS We investigate the relationship between indicators of students' perceived fulfilment of needs and their graded performance to determine whether the connection is indirect via mastery goals. SAMPLE We surveyed 2,105 students during the first year in middle school. METHODS We assessed the amount of the students' perceived autonomy, recognition of competence and support from the teacher (as indicators of competence and social relatedness) in class, their mastery goals, and their grades. Multilevel models were calculated. RESULTS Perceived fulfilment of needs correlated significantly with mastery goals and graded performance. Mastery goals predicted graded performance; however, when perceived fulfilment of needs and mastery goals were analysed simultaneously, the correlation between mastery goals and graded performance was no longer significant. There was no indirect relation between perceived fulfilment of needs and graded performance via mastery goals. CONCLUSIONS Results indicate that creating the learning environment according to the students' basic needs is positively related to their mastery goals and graded performance during the first year at middle school.
Five-Year Outcomes after PCI or CABG for Left Main Coronary Disease.

METHODS We randomly assigned 1905 patients with left main coronary artery disease of low or intermediate anatomical complexity (according to assessment at the participating centers) to undergo either PCI with fluoropolymer-based cobalt–chromium everolimuseluting stents (PCI group, 948 patients) or CABG (CABG group, 957 patients). The primary outcome was a composite of death, stroke, or myocardial infarction.
Role of β-glucosidase 2 in aberrant glycosphingolipid metabolism: model of glucocerebrosidase deficiency in zebrafish[S]

β-glucosidases [GBA1 (glucocerebrosidase) and GBA2] are ubiquitous essential enzymes. Lysosomal GBA1 and cytosol-facing GBA2 degrade glucosylceramide (GlcCer); GBA1 deficiency causes Gaucher disease, a lysosomal storage disorder characterized by lysosomal accumulation of GlcCer, which is partly converted to glucosylsphingosine (GlcSph). GBA1 and GBA2 also may transfer glucose from GlcCer to cholesterol, yielding glucosylated cholesterol (GlcChol). Here, we aimed to clarify the role of zebrafish Gba2 in glycosphingolipid metabolism during Gba1 deficiency in zebrafish (Danio rerio), which are able to survive total Gba1 deficiency. We developed Gba1 (gba1−/−), Gba2 (gba2−/−), and double (gba1−/−:gba2−/−) zebrafish knockouts using CRISPR/Cas9 and explored the effects of both genetic and pharmacological interventions on GlcCer metabolism in individual larvae. Activity-based probes and quantification of relevant glycolipid metabolites confirmed enzyme deficiency. GlcSph increased in gba1−/− larvae (0.09 pmol/fish) but did not increase more in gba1−/−:gba2−/− larvae. GlcCer was comparable in gba1−/− and WT larvae but increased in gba2−/− and gba1−/−:gba2−/− larvae. Independent of Gba1 status, GlcChol was low in all gba2−/− larvae (0.05 vs. 0.18 pmol/fish in WT). Pharmacologic inactivation of zebrafish Gba1 comparably increased GlcSph. Inhibition of GlcCer synthase (GCS) in Gba1-deficient larvae reduced GlcCer and GlcSph, and concomitant inhibition of GCS and Gba2 with iminosugars also reduced excessive GlcChol. Finally, overexpression of human GBA1 and injection of recombinant GBA1 both decreased GlcSph. We determined that zebrafish larvae offer an attractive model to study glucosidase actions in glycosphingolipid metabolism in vivo, and we identified distinguishing characteristics of zebrafish Gba2 deficiency.
Transcriptional downregulation of miR‐127‐3p by CTCF promotes prostate cancer bone metastasis by targeting PSMB5

Prostate cancer (PCa) is one of the most common cancers in males and particularly tends to metastasize to bone. Currently, metastatic bone disease is incurable, and new therapies need to be developed. Our study aims to determine the role of miR‐127‐3p in PCa metastasis to bone. The results demonstrate that miR‐127‐3p is markedly reduced in bone metastasis‐positive PCa tissues relative to that in bone metastasis‐negative PCa tissues. Furthermore, overexpressing miR‐127‐3p inhibits PCa cell invasion and migration in vitro by targeting the proteasome β‐subunit PSMB5. Moreover, CCCTC‐binding factor (CTCF) transcriptionally inhibits miR‐127‐3p by interacting with the miR‐127‐3p promoter. Collectively, this study uncovers a novel mechanism of the CTCF/miR‐127‐3p/PSMB5 axis in promoting PCa bone metastasis, indicating that miR‐127‐3p could function as a promising therapeutic target against bone metastasis.
Wide Field-of-View Ultrafast Curved Array Imaging Using Diverging Waves

Ultrafast ultrasound imaging provides great opportunities for very high frame rate applications, such as shear wave elastography and microvascular imaging. However, ultrafast imaging with curved array transducers remains challenging in terms of element directivity and a limited field-of-view (FOV) for a fully synthetic area. In this paper, a wide FOV ultrafast curved array imaging method based on diverging wave transmissions is presented for high frame rate abdominal ultrasound applications. For this method, a theoretical model for a diverging wave solution based on a virtual point source originating from a circular line is proposed, and the FOV and element directivity are analyzed by this model. Furthermore, an integrated model for plane wave and diverging wave imaging along the location of the virtual point source is derived. The proposed method was evaluated with simulation, phantom, and in vivo studies. In the simulation and phantom studies, the image quality (i.e., spatial resolution, cystic resolution, and contrast-to-noise ratio), and effective FOV were assessed. For the in vivo study, a preliminary result from abdominal microvascular imaging, where diverging wave excitation was utilized to depict the vasculature, was also presented. In the renal cortex microvessels, the diverging wave imaging yielded a higher signal-to-clutter ratio value than the plane wave imaging, i.e., 6.35 vs. 4.26 dB, due to the wider synthetic field. These studies demonstrated that the proposed ultrafast curved array imaging technique based on diverging wave excitation allowed for an extended FOV with high spatiotemporal resolution.
Patients and their family members prioritize post‐transplant survival over waitlist survival when considering donor hearts for transplantation

Heart transplant providers often focus on post‐transplant outcomes when making donor decisions, potentially at the expense of higher waitlist mortality. This study aimed to assess public opinion regarding the selection of donor hearts and the balance between pre‐ and post‐transplant risk. The authors generated a survey to investigate public opinion regarding donor acceptance. The survey was shared freely online across social media platforms in April‐May 2019. A total of 718 individuals responded to the survey, with an equal distribution between patients and family members. Respondents consistently favored post‐transplant outcomes over waitlist outcomes. About 83.9% of respondents favored a hospital with longer waitlist times, worse waitlist outcomes, but excellent post‐transplant survival over a hospital with short waitlist times, a high waitlist survival, and inferior post‐transplant survival. This preference was no different between pediatric and adult populations (P = .7), patient and family members (P = .935), or those with a pre‐ vs post‐transplant perspective (P = .985). Patients and their family members consistently favor improved post‐transplant survival over waitlist survival when considering the risks of accepting a donor organ. These findings suggest that current practice patterns of donor selection align with the opinions of patients and family members with heart failure or who have undergone heart transplantation.
Recovery and reuse of alginate in an immobilized algae reactor

ABSTRACT The use of microalgae for nutrients removal from wastewater has attracted more attention in recent years. More specifically, immobilized systems where algae cells are entrapped in beads in a matrix of a polysaccharide such as alginate have shown a great potential for nutrients removal from wastewater to low levels with reduced retention times and hence smaller footprint. However, a significant operational cost in the up-scaling of alginate-immobilized algae reactors will be the gelling agent alginate. To reduce expenditure of this consumable a proof-of-concept is given for an alginate recycling method using sodium citrate as a dissolving agent. Using algae beads made from virgin and recycled alginate yielded comparable removal rates for both phosphorus and nitrogen compounds from wastewater. At labscale, an alginate recovery of approximately 70% can be achieved which would result in a net operational cost reduction of about 60%. GRAPHICAL ABSTRACT
Physicochemical, Spectroscopic and Chromatographic Analyses in Combination with Chemometrics for the Discrimination of Four Sweet Cherry Cultivars Grown in Northern Greece

A total of 56 sweet cherry samples belonging to four cultivars (Ferrovia, Canada Giant, Lapins, and Germersdorfer) grown in northern Greece were characterized and differentiated according to botanical origin. For the above purpose, the following parameters were determined: conventional quality parameters (titratable acidity (TA), pH, total soluble solids (TSS), total phenolic content (TPC), mechanical properties and sensory evaluation, sugars by High Performance Liquid Chromatography (HPLC), volatile compounds by GC/MS, and minerals by ICP-OES. Statistical treatment of the data was carried out using Multivariate Analysis of Variance (MANOVA) and Linear Discriminant Analysis (LDA). The results showed that the combination of volatile compounds and conventional quality parameters provided a correct classification rate of 84.1%, the combination of minerals and conventional quality parameters 86.4%, and the combination of minerals, conventional quality parameters and sugars provided the highest correct classification rate of 88.6%. When the above four cherry cultivars were combined with previously studied Kordia, Regina, Skeena and Mpakirtzeika cultivars, collected from the same regions during the same seasons, the respective values for the differentiation of all eight cultivars were: 85.5% for the combination of conventional quality parameters, volatiles and minerals; and 91.3% for the combination of conventional quality parameters, volatiles, minerals, and sugars.
Interpersonal problems as a predictor of pain catastrophizing in patients with chronic pain

Abstract Background and aims Pain catastrophizing has consistently been related to a variety of negative outcomes within chronic pain conditions, but competing models exist explaining the role of catastrophizing. According to the fear-avoidance model (FAM), catastrophizing is primarily related to the appraisal of pain (i.e. “intrapersonal”), whereas the communal coping model (CCM) suggests that catastrophizing is a strategy to elicit support (i.e. “interpersonal”). In order to examine the interpersonal nature of catastrophizing, this cross-sectional study examined interpersonal problems as a predictor of pain catastrophizing in a sample of patients (n = 97) with chronic pain. Methods Self-report data was taken from patients entering a multidisciplinary, inpatient rehabilitation program. The four quadrants of the Inventory of Interpersonal Problems circumplex model (Hostile-Dominant, Hostile-Submissive, Friendly-Submissive, Friendly-Dominant) were used as predictors of pain catastrophizing in a series of separate, hierarchical regression analyses. Results After controlling for relevant confounding variables such as demographics (gender, age), pain severity, psychiatric symptoms (anxiety/depression, fatigue, insomnia), adverse life experiences and perceived social support, higher levels of Hostile-Dominant interpersonal problems predicted higher levels of pain catastrophizing (p ≤ 0.01, d = 0.56). Conclusions The results add support to the notion that pain catastrophizing may serve a communicative functioning, as predicted by the CCM, with cold, dominant and controlling behaviors as a maladaptive interpersonal strategy to elicit support. It may thus be useful to consider the broader interpersonal context of the individual, and not only the patient’s appraisal of pain, when conceptualizing the role of pain catastrophizing in patients with chronic pain. Implications Future psychosocial research and treatment of chronic pain could be informed by including interpersonal theory as a useful theoretical framework, which may help shed more light on how interpersonal problems relates to pain catastrophizing.
Overexpression of a Novel Cytochrome P450 Promotes Flavonoid Biosynthesis and Osmotic Stress Tolerance in Transgenic Arabidopsis

Flavonoids are mainly associated with growth, development, and responses to diverse abiotic stresses in plants. A growing amount of data have demonstrated the biosynthesis of flavonoids through multienzyme complexes of which the membrane-bounded cytochrome P450 supergene family shares a crucial part. However, the explicit regulation mechanism of Cytochrome P450s related to flavonoid biosynthesis largely remains elusive. In the present study, we reported the identification of a stress-tolerant flavonoid biosynthetic CtCYP82G24 gene from Carthamus tinctorius. The transient transformation of CtCYP82G24 determined the subcellular localization to the cytosol. Heterologously expressed CtCYP82G24 was effective to catalyze the substrate-specific conversion, promoting the de novo biosynthesis of flavonoids in vitro. Furthermore, a qRT-PCR assay and the accumulation of metabolites demonstrated that the expression of CtCYP82G24 was effectively induced by Polyethylene glycol stress in transgenic Arabidopsis. In addition, the overexpression of CtCYP82G24 could also trigger expression levels of several other flavonoid biosynthetic genes in transgenic plants. Taken together, our findings suggest that CtCYP82G24 overexpression plays a decisive regulatory role in PEG-induced osmotic stress tolerance and alleviates flavonoid accumulation in transgenic Arabidopsis.
Optimal Isolation Method of Small Extracellular Vesicles from Rat Plasma

Small extracellular vesicles (sEVs) mediate cell–to–cell communication. We recently reported that circulating sEVs regulate systolic blood pressure in an animal model of human systemic hypertension. However, the underlying mechanisms still remain to be elucidated. As the first step for detailed analyses, we sought to increase the yield and purity of sEVs isolated from rat plasma. We compared the concentration and size distribution of sEVs as well as protein expression of the sEV marker and contaminants among plasma sEVs isolated by the ultracentrifugation (UC) method, the precipitation with polyethylene-glycol and ultracentrifugation (PEG-UC) method, or the precipitation with polyethylene-glycol (PEG) method. Effects of anticoagulants were also examined. The total concentration of plasma sEVs isolated by the PEG or PEG-UC method was much higher than that of the UC method. In the plasma sEVs isolated by the PEG-UC method, contaminating proteins were lower, while the protein expression of certain sEV markers was higher than that of the PEG method. There was no significant difference in total concentration or protein expression of sEV markers in sEVs isolated from rat plasma treated with three different anticoagulants (heparin, ethylenediaminetetraacetic acid, or acid citrate dextrose buffer) by the PEG-UC method. We, for the first time, determined that the PEG-UC method was optimal for sEV isolation from rat plasma.
Fatal Immunohaemolysis after the Consumption of the Poison Pax Mushroom: A Focus on the Diagnosis of the Paxillus Syndrome with the Aid of Two Case Reports

This retrospective report focuses on the diagnosis of the Paxillus syndrome, based on two fatal cases of haemolysis following the consumption of Paxillus involutus. These mushrooms are still consumed regularly, despite earlier reports of life-threatening autoimmune haemolytic anaemia. Such cases are nevertheless rare, and thus far no toxin could be identified that causes this unusual form of mushroom poisoning. All these factors contribute to the difficulty in diagnosing the Paxillus syndrome. The following aspects support the diagnosis in the two cases presented here: Both patients consumed the mushroom oftentimes before, yet allegedly without ill effects. Symptoms occurred 2–3 h after the last consumption, exacerbating into circulatory collapse, multiorgan failure, and death. Disseminated intravascular coagulation was identified as cause of death by autopsy of patient 1. Patient 2 died of multiorgan failure, mainly hepatic. Our mycological analyses could identify the consumed mushroom in both cases as Paxillus involutus. Furthermore, we could exclude anticoagulants and several other drugs as trigger for the haemolysis by post-mortem toxicological analysis. However, findings in each of the two cases may have led to the haemolysis, independent of the consumption of Paxillus involutus. Patient 1 carried the anti-erythrocytic antibody, auto-anti-e. Patient 2 contracted chronic hepatitis C years prior to the current incident. Considering the rarity of the Paxillus syndrome, our findings suggest that these patients were particularly susceptible for haemolysis after consuming this mushroom over a prolonged period. Occurrence of the Paxillus syndrome may thus be restricted to regular consumers of Paxillus involutus mushrooms with an existing predisposition for haemolysis.
A forecasting tool for prediction of epileptic seizures using a machine learning approach

ECG and EEG signals are very helpful in the early diagnosis of epileptic seizures. The research focuses on analysis of ECG and EEG signals applying a deep learning technique to study early prediction of epileptic seizure. Signal processing methods like Empirical Mode Decomposition, spectral analysis, and statistical methods were used. The algorithms were implemented in MATLAB, and the EEG and ECG data were collected from Physiobank and EPILEPSIAE databases. In the window‐based analysis of low‐frequency spectral area of EEG signals, 78.5% of the cases displayed a significant change as the windows progressed and the onset of seizure was approached. The spectral area of IMF components indicated a possible seizure prediction in 68.9% of the analyzed cases. Considering signals from individual EEG electrodes, the least percentage of seizure prediction was indicated by signals from T4 and F4 electrodes (52.3% and 40.7%, respectively, for spectral peaks and 23.8% and 29.6%, respectively, for spectral area). The results of regression analysis show that prediction of seizures can be possible around 20‐30 minutes prior to the actual occurrence of seizures.
Title : Is perivitelline space morphology of the oocyte associated with pregnancy outcome in intracytoplasmic sperm injection cycles ?

Objective(s): To investigate the effect of perivitelline space (PVS) abnormalities on the outcomes of intracytoplasmic sperm injection (ICSI) cycles in which the entire cohort was affected. Study Design: Data from 9,752 oocytes obtained from 1,151 ICSI cycles performed from June/2010 to August/2016 in a private university-affiliated IVF centre. Cycles were divided into four groups according to the presence or absence of PVS abnormalities: PVS-L group (cycles with the entire oocyte cohort affected by large PVS, n=265), PVS-G group (cycles with the entire oocyte cohort affected by PVS granularity, n=280), PVS-L + PVS-G group (cycles with the entire oocyte cohort affected by PVS-L and PVS-G, n=204), and control group (cycles with the entire oocyte cohort free of PVS abnormalities, n=402). The effect of PVS abnormalities on ICSI outcomes was assessed by GLM adjusted for potential confounders. Results: Groups with PVS abnormalities presented substantially higher FSH/follicle (p<0.001) and FSH/oocyte (p<0.001) ratios, and lower numbers of follicles (p<0.001), oocytes (p<0.001) and embryos (p=0.002) compared to the control group. PVS-L + PVS-G implantation (p=0.044) and pregnancy (p=0.004) rates were significantly lower than in cycles with isolated PVS abnormalities and controls. Conclusion(s): Cycles in which the entire oocyte cohort is affected by both large PVS and PVS granularity have compromised implantation and pregnancy rates.