Miracle-dz/UniFAR-FTUs huggingface dataset:
Connexin45 mediates gap junctional coupling of bistratified ganglion cells in the mouse retina

Direction selectivity, a key feature of visual perception, originates in the retina and is transmitted by bistratified ganglion cells that, in the rabbit retina, exhibit a particular coupling pattern. We intracellularly labeled ganglion cells in different transgenic mouse lines, allowing a morphological classification of bistratified ganglion cells, an analysis of their coupling pattern, and the molecular identification of the connexins responsible for the coupling. Based on dendritic characteristics including co‐fasciculation with the dendrites of cholinergic starburst amacrine cells, we were able to distinguish three types of bistratified ganglion cells. Two of these co‐fasciculate with starburst amacrine cells and exhibit a specific homologous coupling pattern. Connexin45 (Cx45) appears to be the major component of the gap junctional channels because tracer coupling is absent in Cx45‐deficient animals whereas it persists in Cx36‐deficient animals. It is speculated that the transjunctional voltage dependence of Cx45 channels could support the transmission of direction selectivity. J. Comp. Neurol. 490:29–39, 2005. © 2005 Wiley‐Liss, Inc.
Significance of keratinized mucosa in maintenance of dental implants with different surfaces.

BACKGROUND The need for keratinized mucosa (KM) or immobile keratinized mucosa (i.e., attached mucosa [AM]) for the maintenance of osseointegrated endosseous dental implants has been controversial. The purpose of this study was to investigate the significance of KM in the maintenance of root-form dental implants with different surfaces. METHODS A total of 339 endosseous dental implants in place for at least 3 years in 69 patients were evaluated. The width of KM and AM, modified plaque index (mPI), gingival index (GI), modified bleeding index (mBI), probing depth (PD), and average annual bone loss (ABL) were measured clinically and radiographically by a masked examiner. Based on the amounts of KM or AM, implants were categorized as follows: 1) KM <2 mm (KL); 2) KM > or =2 mm (KU); 3) AM <1 mm (AL); and 4) AM > or =1 mm (AU). Implants were further subdivided into the following four subgroups based on their surface configurations: 1) smooth surface implants (SI) with KM <2 mm (SKL); 2) SI with KM > or =2 mm (SKM); 3) rough surface implants (RI) with KM <2 mm (RKL); or 4) RI with KM > or =2 mm (RKM); or 1) SI with AM <1 mm (SAL); 2) SI with AM > or =1 mm (SAM); 3) RI with AM <1 mm (RAL); or 4) RI with AM > or =1 mm (RAM). The effect of KM or AM on clinical parameters was evaluated by comparing the different KM/AM groups. In addition, the significance of the presence of KM on implant prostheses types (i.e., fixed versus removable) and on implant locations (i.e., anterior versus posterior) was evaluated. RESULTS Comparison of ABL among the four subgroups in KM or AM failed to reveal statistically significant differences (P >0.05); however, statistically significantly higher GI and mPI were present in SKL or SAL compared to the other three subgroups (P <0.05). GI and mPI were significantly higher in KL (0.94 and 1.51) than KU (0.76 and 1.26) and higher in AL (0.95 and 1.50) than AU (0.70 and 1.19) (P <0.05), respectively. The difference in GI between posterior implants with or without an adequate amount of KM was also significant (P <0.05). CONCLUSIONS The absence of adequate KM or AM in endosseous dental implants, especially in posterior implants, was associated with higher plaque accumulation and gingival inflammation but not with more ABL, regardless of their surface configurations. Randomized controlled clinical trials are needed to confirm the results obtained in this retrospective clinical study.
Association between bipolar spectrum features and treatment outcomes in outpatients with major depressive disorder.

CONTEXT It has been suggested that patients with major depressive disorder (MDD) who display pretreatment features suggestive of bipolar disorder or bipolar spectrum features might have poorer treatment outcomes. OBJECTIVE To assess the association between bipolar spectrum features and antidepressant treatment outcome in MDD. DESIGN Open treatment followed by sequential randomized controlled trials. SETTING Primary and specialty psychiatric outpatient centers in the United States. PARTICIPANTS Male and female outpatients aged 18 to 75 years with a DSM-IV diagnosis of nonpsychotic MDD who participated in the Sequenced Treatment Alternatives to Relieve Depression (STAR*D) study. INTERVENTIONS Open treatment with citalopram followed by up to 3 sequential next-step treatments. MAIN OUTCOME MEASURES Number of treatment levels required to reach protocol-defined remission, as well as failure to return for the postbaseline visit, loss to follow-up, and psychiatric adverse events. For this secondary analysis, putative bipolar spectrum features, including items on the mania and psychosis subscales of the Psychiatric Diagnosis Screening Questionnaire, were examined for association with treatment outcomes. RESULTS Of the 4041 subjects who entered the study, 1198 (30.0%) endorsed at least 1 item on the psychosis scale and 1524 (38.1%) described at least 1 recent maniclike/hypomaniclike symptom. Irritability and psychoticlike symptoms at entry were significantly associated with poorer outcomes across up to 4 treatment levels, as were shorter episodes and some neurovegetative symptoms of depression. However, other indicators of bipolar diathesis including recent maniclike symptoms and family history of bipolar disorder as well as summary measures of bipolar spectrum features were not associated with treatment resistance. CONCLUSION Self-reported psychoticlike symptoms were common in a community sample of outpatients with MDD and strongly associated with poorer outcomes. Overall, the data do not support the hypothesis that unrecognized bipolar spectrum illness contributes substantially to antidepressant treatment resistance.
Efficient design of non-binary LDPC codes for magnetic recording channels, robust to error bursts

In this work we consider an optimized design of q-ary low-density parity-check (LDPC) codes that takes into account their burst error correction capability. In recent works, the performance of LDPC decoding in presence of noise bursts has been related to the structure of the parity-check matrix. In particular, two approaches to characterize the burst error correction capabilities have been proposed in the literature. Following these ideas, we compare different matrix designs in order to choose the best matrix constraints to be maximized in a PEG construction. Several non-binary LDPC codes, generated with the proposed design methods are compared. Their performance are analyzed in the context of magnetic recording channels, where they are considered a promising alternative to the Reed Solomon (RS) codes.
Differential expression of proteins from cultured endothelial cells exposed to uremic versus normal serum.

BACKGROUND Deficient hemostasis and accelerated atherosclerosis coexist in patients with chronic kidney disease. Endothelial dysfunction may be involved in the high incidence of atherothrombotic events in these patients. We established an in vitro model of endothelial dysfunction by exposing endothelial cells to uremic media and applied a proteomic approach to characterize endothelial cell dysfunction in uremia. STUDY DESIGN Cross-sectional study. SETTING AND PARTICIPANTS Serum samples from 8 patients with chronic kidney disease on hemodialysis treatment were collected. PREDICTOR Exposure of cultured endothelial cells to normal and uremic serum. OUTCOME AND MEASUREMENTS: Proteins from lysed cells were characterized by isoelectric point and molecular weight by using 2-dimensional gel electrophoresis. Spots were visualized by means of silver staining and identified by using mass spectrometry. RESULTS Identification of the most prominent proteins showed molecules related to inflammation (high mobility group box 1, aldose reductase, and proteasome components) and oxidative stress (superoxide dismutase and glutathione peroxidase), both associated with chronic kidney disease. These changes may be caused by activation of the nuclear factor-kappaB transcription factor. Changes in expression of cytoskeletal proteins (destrin and vimentin) also were detected. LIMITATIONS In vitro study. CONCLUSION Proteomic techniques proved to be a powerful tool to investigate endothelial dysfunction in uremia. A more exhaustive analysis will provide answers and potential therapeutic targets in the near future.
Using stable nitrogen-isotopes to study weaning behavior in past populations.

Abstract Several different methods have been used to explore weaning behavior in past populations, including demographic profiles, non‐specific osteological indicators of stress, and bone chemistry studies. Stable nitrogen‐isotope ratios of prehistoric bone proteins provide an especially useful method for reconstructing the weaning patterns of archaeological populations. A demographic measure of fertility (the D30+/D5+ ratio) is compared with age‐related changes in stable nitrogen‐isotope ratios for human burials from one historic cemetery and two prehistoric ones. The stable nitrogen‐isotope ratios show that each population had a characteristic and distinctive combination of weaning time and rate, and that neither the timing nor tempo of weaning was clearly correlated with the demographic measure of fertility. These results demonstrate the feasibility of using stable nitrogen‐isotope ratios to compare weaning patterns and fertility in past populations. This example also shows that the relationship betwee.
Fertility-Sparing Therapy in Young Women With Endometrial Cancer: 2010 Update

Endometrial cancer (EC) in young and especially null gravid women is a challenging problem with increasing frequency due to both detailed investigations for infertile couples seeking treatment in in vitro fertilization centers and women delaying their plans for pregnancy. Considering very low number of such cases in the literature, fertility-sparing therapy for EC in young women is not standard, although it is widely used. In this review we searched the relevant literature, reviewed selection criteria, therapeutic options, response rates to therapy, special challenges, reproductive outcomes, and follow-up strategies for young women diagnosed with EC.
Acute Tubular Injury in Protocol Biopsies of Renal Grafts: Prevalence, Associated Factors and Effect on Long‐Term Function

Acute tubular injury (ATI) is commonly observed in renal allografts, especially early after transplantation. This study analyzes prevalence and associated clinical conditions of ATI in serial protocol biopsies (pBx) and indication biopsies (iBx), and its impact on long‐term graft function.
Are reproduction impairments of free spawning marine invertebrates exposed to zero-valent nano-iron associated with dissolution of nanoparticles?

Abstract Studies were carried out to assess the effects of coating applied to zero-valent nano-iron (nZVI) on early life stage development of three key marine invertebrate species Mytilus galloprovincialis, Ciona intestinalis and Psammechinus milliaris. Embryo development was assessed following a 2-h exposure of the sperm to concentrations of two nZVIs of up to 10 mg l-1 followed by in vitro fertilisation. Disruption of embryo development was most severe in sea squirts followed by mussel, while the urchin embryos were not significantly affected as compared with controls. An over twofold decrease in fertilisation success alongside significant delay in the embryo development was observed, and the effect was more severe with the coated form, possibly owing to its better colloidal stability. We provide in vitro evidence for the rapid dissolution (within 2 h) of nZVI in seawater to a degree that concentration of total solute Fe released from the coated ZVI particles exceeds safe limits of NOECs established for dissolved Fe.
SOLAR ENERGY AND ITS BIOLOGICAL – PHYSICAL INTERACTIONS IN THE SEA

For over 4 billion years, solar radiation has played a profound role in driving the physical circulation of the atmosphere, the oceans, and the biological processes responsible for sustaining life on Earth. The angular distribution of, and diel and seasonal changes in, solar radiation are dictated by the relation of Earth’s axis to the Sun, the orbital angular velocity of Earth, and the gravitational interactions between Earth, the Sun, and the neighboring planets. To a large extent, these physical interactions are deterministic and have been sustained throughout Earth’s history. The angular distribution of radiant energy and the seasonal variations in solar radiation drive large-scale thermohaline circulation of the ocean. This circulation pattern is manifested in an equator-to-pole heat transport superimposed on the surface of a rotating sphere. The meridional transport of heat and formation of deep water at high latitudes are dependent on the angular distribution of solar radiation and continental configuration. On geological time scales there is strong evidence of large thermal changes in both surface and deep waters that clearly must have affected nutrient fluxes, the
Bargaining Over a New Welfare State

The goal of this paper is twofold: First, to develop an estimable model of legislative politics in the US Congress, second, to provide a greater understanding of the objectives behind the New Deal. In the theoretical model, the distribution of federal funds across regions of the country is the outcome of bargaining game in which the President acts as the agenda-setter and Congress bargains over the final shape of the spending bill. For any given preferences (of the President) and distribution of seats in Congress, the model delivers a unique predicted allocation. Combined with data on New Deal programs, this is used to estimate the objectives of the Roosevelt administration. The results indicate that economic concerns for relief and recovery, though not necessarily for fundamental reform and development, largely drove New Deal spending. Political concerns also mattered, but more on the margin.
Pharmacokinetics and pharmacodynamics of single subcutaneous doses of tocilizumab administered with or without rHuPH20.

OBJECTIVES To investigate the safety, tolerability, pharmacokinetics (PK), and pharmacodynamics (PD) of tocilizumab with and without rHuPH20 (a recombinant human hyaluronidase) in healthy volunteers. METHODS This was an open-label, single ascending dose study. Subjects were assigned to tocilizumab 162 mg or tocilizumab 162, 324, or 648 mg plus rHuPH20. PK and PD samples were collected after dosing and were estimated with non-compartmental methods. Geometric mean ratio (GMR) for area under the plasma concentration-time curve from zero to infinity (AUC0-∞) and (maximum serum concentration) Cmax with and without rHuPH20 was estimated using one-way analysis of variance. Safety and tolerability were monitored throughout the study. RESULTS 48 subjects (12/cohort) received a single dose of tocilizumab with or without rHuPH20. For tocilizumab 162 mg, tocilizumab 162 mg/rHuPH20, tocilizumab 324 mg/rHuPH20, and tocilizumab 648 mg/rHuPH20, mean ± SD tocilizumab PK parameters were 2,510 ± 1,060, 2,860 ± 468, 10,800 ± 3,220, and 29,900 ± 5,280 μg×h/ml for AUC0-∞; 11.5 ± 3.7, 16.2 ± 2.8, 43.8 ± 12.4, and 77.8 ± 14.5 μg/ml for Cmax; and 89.1 ± 41.1, 54.0 ± 19.5, 66.0 ± 26.8, and 86.1 ± 50.6 h for tmax, respectively. Coadministration of tocilizumab 162 mg with rHuPH20 resulted in slightly increased exposure: GMR (90% confidence interval) for AUC0-∞, 1.20 (1.00 - 1.44) and Cmax, 1.45 (1.24 - 1.70). Increasing tocilizumab doses resulted in significant deviation from dose proportionality for tocilizumab Cmax (p = 0.0057) and AUC0-∞ (p < 0.0001). Changes in interleukin-6, soluble interleukin- 6 receptor, and C-reactive protein were also dose dependent and similar with and without rHuPH20. CONCLUSIONS Tocilizumab in combination with rHuPH20 resulted in slightly increased tocilizumab exposure compared with tocilizumab alone, whereas PD markers were comparable. Subcutaneous administration of tocilizumab with rHuPH20 was well tolerated.
The Influence of Epoch Length on Physical Activity Patterns Varies by Child's Activity Level

Purpose: Patterns of physical activity (PA) and sedentary time, including volume of bouted activity, are important health indicators. However, the effect of accelerometer epoch length on measurement of these patterns and associations with health outcomes in children remain unknown. Method: We measured activity patterns in 308 children (52% girls, age range = 8–11 years) using ActiGraph GT1M accelerometers with 15-s epochs and reintegrated to 60-s epochs. We calculated the volume (minutes per day) of moderate-to-vigorous PA (MVPA), sedentary time, light, moderate, and vigorous PA, as well as bouted MVPA and sedentary time (0–5 min, 5–10 min, 10–20 min, and > 20 min). Results: The difference between 15-s and 60-s epochs was statistically significant for all outcomes; however, effect sizes were small or negligible in 30% of comparisons. Bias ranged from 1.9 min/day (total MVPA) to 102.7 min/day (0–5 min sedentary bouts). Regression-based estimates of bias and 95% limits of agreement illustrated that the magnitude, and in some cases, the direction, of between-epoch differences varied with activity level. Correlations with body mass index and cardiovascular fitness were similar for 15-s (r = − .19 to .20) and 60-s (r = − .16 to .29) epochs. Estimated 15-s data (predicted from 60-s) were similar to measured data and had similar relationships with health outcomes. Conclusion: Epoch length influences measurement of PA and sedentary patterns and the effect is modified by activity level. However, associations with health outcomes were similar and epoch differences can be adjusted. Future research should clarify the accuracy of different epoch lengths for measuring bouted activity and evaluate whether epoch length alters relationships with additional health outcomes.
Secondary production, longevity and resource consumption rates of freshwater shrimps in two tropical streams with contrasting geomorphology and food web structure

SUMMARY 1. Freshwater shrimps often dominate the biomass of tropical island streams and are known to have strong effects on stream ecosystem structure and function, but little effort has been dedicated toward quantifying basic energetic and life history attributes such as growth, production and longevity. Such information is critical for understanding both the role of shrimps in ecosystem dynamics and the gravity of threats to shrimp populations posed by human activities such as shrimp harvesting, dam construction and water withdrawal. 2. We quantified growth rates and secondary production of dominant freshwater shrimps for 3 years in two Puerto Rican headwater streams that differ in food web structure because of the presence or absence of predatory fishes that are excluded from reaches above waterfalls. Using growth data, we constructed a minimum longevity model to explore the likely minimum life spans of the two dominant taxa (Atya spp. and Xiphocaris elongata). Finally, we used a bioenergetics model to quantify annual consumption rates of major basal resources by the two taxa. 3. Daily growth rates ranged from )0.001 to 0.011 day )1 , were inversely related to body size, and were higher for small individuals of X. elongata than Atya spp. Mean annual shrimp biomass and secondary production were an order of magnitude higher in the stream that lacked predatory fishes (biomass: 4.34 g AFDM m )2 ; production: 0.89 g AFDM m )2 year )1 ) than in the stream with predatory fishes (biomass: 0.12 g AFDM m )2 ; production: 0.02 g AFDM m )2 year )1 ). Production : biomass ratios ranged from 0.01 to 0.38. 4. Our longevity model predicted a minimum life span of 8 years for Atya spp. and 5 years for X. elongata in the stream lacking predatory fishes. In contrast, due to a larger average size of X. elongata in the stream with predatory fishes, our model predicted a minimum life span of 11 years. Actual life spans of these taxa are likely to be much longer based on long-term observations of marked individuals.
An Array of Coactivators Is Required for Optimal Recruitment of TATA Binding Protein and RNA Polymerase II by Promoter-Bound Gcn4p

ABSTRACT Wild-type transcriptional activation by Gcn4p is dependent on multiple coactivators, including SAGA, SWI/SNF, Srb mediator, CCR4-NOT, and RSC, which are all recruited by Gcn4p to its target promoters in vivo. It was not known whether these coactivators are required for assembly of the preinitiation complex (PIC) or for subsequent steps in the initiation or elongation phase of transcription. We find that mutations in subunits of these coactivators reduce the recruitment of TATA binding protein (TBP) and RNA polymerase II (Pol II) by Gcn4p at ARG1, ARG4, and SNZ1, implicating all five coactivators in PIC assembly at Gcn4p target genes. Recruitment of Pol II at SNZ1 and ARG1 was eliminated by mutations in TBP or by deletion of the TATA box, indicating that TBP binding is a prerequisite for Pol II recruitment by Gcn4p. However, several mutations in SAGA subunits and deletion of SRB10 had a greater impact on promoter occupancy of Pol II versus TBP, suggesting that SAGA and Srb mediator can promote Pol II binding independently of their stimulatory effects on TBP recruitment. Our results reveal an unexpected complexity in the cofactor requirements for the enhancement of PIC assembly by a single activator protein.
Interim analysis of a prospective phase I/II trial of SBRT for liver metastases

Stereotactic Body Radiation Therapy (SBRT) is a potent means of systemic cytoreductive therapy for selected patients with metastatic cancer. We here report an interim analysis of a prospective Phase I/II study of SBRT for liver metastases. Eligible patients with liver metastases met these criteria: (1) maximum tumor diameter < 6 cm; (2) ≤3 discrete lesions; (3) treatment planning confirmed ≥ 700 cm3 of normal liver receives ≤15Gy. The gross tumor volume (GTV) was expanded 5–10 mm to yield the planning target volume, which received 60 Gy in 3 fractions of SBRT over 3–14 days in the Phase II component of the trial. As of July, 2006, 36 patients have been enrolled: 18 in Phase I, 18 in Phase II. The median age was 58 years (range 27–91); the M:F ratio was 20:16. The most common primary sites were lung (n = 10), colorectal (n = 9), and breast (n = 4). Among 21 pts with ≥ 6 months post-SBRT follow-up (median 19 months, range 6–29), one instance of SBRT-related grade 3 toxicity occurred in subcutaneous tissue superficial to the liver. No grade IV toxicity occurred. For 28 discrete lesions treated (median GTV 14 cm3, range 1–98) the 18 month actuarial local control estimate is 93%. This interim analysis indicates that a very high rate of durable in-field tumor control can be safely achieved with SBRT to 1–3 liver lesions as administered in this protocol, to a prescription dose of 60 Gy in 3 fractions.
Investigation of BOLD contrast in fMRI using multi‐shot EPI

High resolution volume coverage of the brain in fMRI studies can be accomplished using multi‐slice, multi‐shot EPI. We demonstrate that the optimum contrast sensitivity in BOLD based fMRI experiments using multi‐shot EPI is achieved by using the shortest possible TR values beween segments and the longest tolerable echo train length. For a fixed paradigm time, acquiring the largest number of images under these conditions yields the best statistical detection thresholds. For a multi‐slice multi‐shot EPI fMRI study, the best approach is to perform the acquisition of all the segments in a single slice before continuing on to each of the slices in turn. Our results regarding the number of shots and TR are also applicable to other multi‐shot techniques such as interleaved spirals. © 1997 John Wiley & Sons, Ltd.