Miracle-dz/UniFAR-FTUs huggingface dataset:
ACE-Inhibitory Procyanidins from Lespedeza capitata

Extracts of Lespedeza capitata Michx. (Fabaceae) have been reported to show diuretic activity and to enhance the renal excretion of nitrogenous compounds of persons with hyperazotemia (1). Until now, phytochemical studies on Lespedeza capitata leaves have been limited to the isolation of flavonoids (2, 3). In the course of an in vitro screening program for inhibitory activity on angiotensin I-converting enzyme (ACE) (4), which has been established for detecting potential anti-hypertensive plant constituents, the ethyl acetate fraction of the ethanol extract obtained from the leaves
Marriage, Divorce, and Alcohol Use in Young Adulthood

Marital status is a robust predictor of alcohol consumption in young adulthood; however, the extent to which observed associations are due to socialization or selection processes is unclear. The current study examined associations between marital status and alcohol use, assessed in a sample of 5,150 young adults (ages 18–30) from the National Longitudinal Survey of Youth. A longitudinal sibling-comparison design controlled for family-level environmental and genetic selection factors and for an individual’s premarital trajectory of alcohol use. Nested model comparisons tested whether gender and age moderated the effects of marriage and divorce. Controlling for selection factors, the transition into marriage predicted decreases in alcohol consumption, and this effect was consistent across gender and age. Divorce predicted increased consumption, particularly for men. Findings support a causal relationship between changes in marital status and alcohol use, rather than an association due to selection factors and suggest gender-specific changes in alcohol use following divorce.
Humeral locking nail: a preliminary report.

Cette technique diminue le temps operatoire, conduit a la formation precoce d'un cal osseux, permet un traitement fonctionnel immediat, le principe de l'enclouage, la technique operatoire, le materiel, le traitement postoperatoire et les resultats chez 80 patients, sont presentes
Purification and characterization of an oxygen-labile, NAD-dependent alcohol dehydrogenase from Desulfovibrio gigas

A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (Km, 0.15 mM) and 1-propanol (Km, 0.28 mM). N-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.
Shortage of donation despite an adequate number of donors: a professional attitude?

Background. A major problem in the field of transplantation is the persistent shortage of donor organs and tissues for transplantation. This study was initiated to (1) chart the donor potential for organs and tissue in The Netherlands and (2) to identify factors influencing whether donation is discussed with next of kin. Methods. A registration form was constructed to obtain information at time of death of patients about the demographic characteristics, diagnosis, and medical suitability for donation. A prospective study was conducted among 11 hospitals in The Netherlands that gathered 4,877 filled-in forms equaling 8% to 10% of the people dying in a hospital in The Netherlands per year. Results. In the year of the study, organs were retrieved from 22 donors and tissues from 264 donors in the 11 hospitals. The organ potential is estimated at a maximum of 38.7 per million population per year. A mere 5% of the physicians got a 100% score on criteria and contraindications for donation. Factors of influence on receiving consent for donation were the will of the donor, using a protocol, giving verbal information to the relatives, and presence of the partner of the deceased patient. For 26% of the potential tissue donors and 69% of the potential organ donors, donation was discussed with the relatives. Consent for tissue donation was obtained in 27%, and consent for organ donation was obtained in 60%. Conclusions. In The Netherlands, when taking into account current refusal percentages, 320 to 360 organ donations and 5,800 tissue donations could be effectuated if organ donation is posed to all possible donors. For this, knowledge of medical criteria and contraindications for donation by the physicians and their willingness to discuss donation with next of kin must be improved.
Conceptualising Recovery in Mental Health Rehabilitation

Recovery as a concept has gained increased attention in the field of mental health. There is an expectation that service providers use a recovery framework in their work. This raises the question of what recovery means, and how it is conceptualised and operationalised. It is proposed that service providers approach the application of recovery principles by considering systematically individual recovery goals in multiple domains, encompassing clinical recovery, personal recovery, social recovery and functional recovery. This approach enables practitioners to focus on service users' personal recovery goals while considering parallel goals in the clinical, social, and role-functioning domains. Practitioners can reconceptualise recovery as involving more than symptom remission, and interventions can be tailored to aspects of recovery of importance to service users. In order to accomplish this shift, practitioners will require effective assessments, access to optimal treatment and care, and the capacity to conduct recovery planning in collaboration with service users and their families and carers. Mental health managers can help by fostering an organisational culture of service provision that supports a broader focus than that on clinical recovery alone, extending to client-centred recovery planning in multiple recovery domains.
Analysis of the Capital Structure of Ecuadorian Pharmaceutical Companies Impact of Covid-19 on Debt Levels

This paper analyses the variables influencing the capital structure of Ecuadorian pharmaceutical companies, which have experienced variations in their internal design in recent years, and evaluates the effect of the covid-19 pandemic on their debt levels. The leverage ratio is the proxy for capital structure with independent variables as growth, percentage of fixed assets (tangibility), profitability (ROA), firm size, and liquidity. Including a dichotomous variable to assess the effect of covid-19 in 2020. The panel data model helps to analyze the data varying across firms and years. The coefficients are estimated using the Pooled Ordinary Least Squares (POLS) method and the fixed-effects method. The results show that the profitability and liquidity variables are negatively correlated. While the growth, tangibility, and firm size variables are not statistically significant. During 2020 the covid-19 pandemic did not significantly influence the financial statements of the sector. In general, correct management of observed debt levels would allow the industry to face possible external shocks and not present payment problems in the short term.
Markedly elevated serum alkaline phosphatase level in an uncomplicated pregnancy

In normal pregnancy, although maternal serum alkaline phosphatase increases and reaches the two-fold of non-pregnant serum level, above this level, hepatic, renal, osteal diseases and malignancies should be kept in mind. We report a case of 28-year-old pregnant woman with increased maternal serum level of alkaline phosphatase activity (17-fold) due to an increased level of placental isozyme. She delivered vaginally a healthy female baby. After delivery, maternal serum alkaline phosphatase activity returned to normal reference range. The aim of this case report is to show this exaggerated isozyme activity of alkaline phosphatase in pregnancy, and to differentiate it from other causes of elevated level of alkaline phosphatase.
Nutritional value and consumption of black ants (Carebara vidua Smith) within the Lake Victoria region in Kenya.

The edible insects of the Lake Victoria region which provided food and medicine, have suffered the effects of mismanaged environment. Our case study of Carebara vidua Smith (black ant) which is an endangered insect currently threatened with extinction due to human's activities, have provided unique source of protein and medicinal value. C. vidua is an endangered species of Heminoptera. This paper discusses the nutritional value and medicinal potential of the black ant. It is one of the most sought after edible insects because of its nutritional and medicinal value. The samples were collected from Kisumu and Siaya counties along the Lake Victoria region. Standard nutrient analysis methods were used to determine the nutritional value. The insect has between 39.79 to 44.64% protein and about 42.07 to 49.77% fat content depending on the body part. The insect is also rich in iron, zinc, magnesium, potassium and phosphorus. From the fatty acid profile, the edible insects recorded high content of Palmitic, Oleic and Linoleic acids. No Linolenic acid was found in the samples analysed. The elderly Luos of Kenya collect and consume the black ants to manage several body ailments probably due to the essential nutrients found in the insect. C. vidua Smith is fairly similar to Polyrhachis vicina Roger in China which has been processed and commercialised as medicinal to manage several chronic diseases. Further research is needed to highlight the potential medicinal value of C. vidua Smith in Kenya and to save the insect from total disappearance.
Nurse‐coordinated collaborative disease management improves the quality of guideline‐recommended heart failure therapy, patient‐reported outcomes, and left ventricular remodelling

Heart failure (HF) pharmacotherapy is often not prescribed according to guidelines. This longitudinal study investigated prescription rates and dosages of angiotensin‐converting enzyme inhibitors/angiotensin receptor blockers (ACEi/ARB), beta‐blockers, and mineralocorticoid receptor antagonists (MRA), and concomitant changes of symptoms, echocardiographic parameters of left ventricular (LV) function and morphology and results of the Short Form‐36 (SF‐36) Health Survey in participants of the Interdisciplinary Network Heart Failure (INH) programme.
Impact of mild heat stress on dry matter intake, milk yield and milk composition in mid-lactation Holstein dairy cows in a temperate climate

The aim of the present study was to evaluate the impact of summer temperatures in a temperate climate on mid-lactation Holstein dairy cows. Therefore, a data set was examined comprising five trials with dairy cows conducted at the experimental station of the Friedrich-Loeffler-Institute in Braunschweig, Germany. The temperature–humidity index (THI) was calculated using temperature and humidity data from the barns recorded between January 2010 and July 2012. By using a generalised additive mixed model, the impact of increasing THI on dry matter intake, milk yield and milk composition was evaluated. Dry matter intake and milk yield decreased when THI rose above 60, whilst water intake increased in a linear manner beyond THI 30. Furthermore, milk protein and milk fat content decreased continuously with increasing THI. The present results revealed that heat stress exists in Lower Saxony, Germany. However, further research is necessary to describe the mode of action of heat stress. Especially, mild heat stress has to be investigated in more detail and appropriate heat stress thresholds for temperate climates have to be developed.
Knowledge, attitude, and practice of general practitioners in Dammam, Saudi Arabia towards Vitamin D supplementation to infants

Background: With the report of high prevalence rate of Vitamin D deficiency in the literature since the 1980s, the condition can be considered an epidemic in Saudi Arabia. However, no specific steps have been taken by the Ministry of Health to tackle the problem. Moreover, little is known about general practitioners′ (GPs) knowledge, attitude, and practice (KAP) of Vitamin D supplementation for infants. Objectives: To measure the KAP of GPs, of Vitamin D supplementation for infants in Dammam, Saudi Arabia. Materials and Methods: This was a cross-sectional study of GPs practicing in primary healthcare centers in the Dammam area of Saudi Arabia. All the 110 GPs working in primary health centers (PHCs) in Dammam were invited to participate in the study. Data was collected with the help of a self-administered questionnaire. Data was analyzed using SPSS 20.0. Statistical analysis included descriptive analysis of all the variables, expressed as the mean (standard deviation [SD]) or the median if not normally distributed. Results: Ninety-three GPs working in the Dammam area participated in the study. Thirty percent had excellent knowledge of Vitamin D. Ninety two percent had a positive attitude toward Vitamin D supplementation and sixty percent regularly prescribed Vitamin D supplementation for infants. Conclusion: The majority of GPs had a positive attitude toward Vitamin D supplementation for infants. However, their knowledge and practice need improvement.
Autosomal recessive distal renal tubular acidosis associated with Southeast Asian ovalocytosis.

BACKGROUND A defect in the anion exchanger 1 (AE1) of the basolateral membrane of type A intercalated cells in the renal collecting duct may result in a failure to maintain a cell-to-lumen H+ gradient, leading to distal renal tubular acidosis (dRTA). Thus, dRTA may occur in Southeast Asian ovalocytosis (SAO), a common AE1 gene abnormality observed in Southeast Asia and Melanesia. Our study investigated whether or not this renal acidification defect exists in individuals with SAO. METHODS Short and three-day NH4Cl loading tests were performed in 20 individuals with SAO and in two subjects, including their families, with both SAO and dRTA. Mutations of AE1 gene in individuals with SAO and members of the two families were also studied. RESULTS Renal acidification in the 20 individuals with SAO and in the parents of the two families was normal. However, the two clinically affected individuals with SAO and dRTA had compound heterozygosity of 27 bp deletion in exon 11 and missense mutation G701D resulting from a CGG-->CAG substitution in exon 17 of the AE1 gene. Red cells of the two subjects with dRTA and SAO and the family members with SAO showed an approximate 40% reduction in sulfate influx with normal 4,4'-di-isothiocyanato-stilbene-2,2'-disulfonic acid sensitivity and pH dependence. CONCLUSION These findings suggest that compound heterozygosity of abnormal AE1 genes causes autosomal recessive dRTA in SAO.
Comparing effects of clonazepam and zolpidem on sleep quality of patients on maintenance hemodialysis.

INTRODUCTION Poor sleep quality is very common among maintenance hemodialysis patients and has negative impacts on patients' quality of life. Benzodiazepines have traditionally been used in this population; however, they may induce physical dependence and sleep apnea. Nonbenzodiazepine hypnotic medications with less side effects are introduced as alternatives. This study was designed to compare the effect of zolpidem and clonazepam on sleep quality of hemodialysis patients. MATERIALS AND METHODS In a randomized crossover study on 23 hemodialysis patients, sleep quality was assessed using the Pittsburgh Sleep Quality Index at baseline, at the initiation of a 1-week washout period after a 2-week treatment with zolpidem (1 mg) and clonazepam (5 mg to 10 mg), and after the second 2 weeks of treatment. Patients who suffer from any concurrent situations that may affect sleep quality or psychiatric disorders and those on medications affecting sleep quality were excluded. RESULTS The prevalence of poor sleep quality was 87.8% of the 88 hemodialysis patients who were initially approached. There was a significant negative correlation between iron deficiency and poor sleep quality. Both clonazepam and zolpidem significantly improved sleep quality; however, clonazepam was more effective in decreasing the Pittsburgh Sleep Quality Index scores (P = .03). Zolpidem was better tolerated in the hemodialysis patients. CONCLUSIONS Clonazepam was more effective than zolpidem in the improvement of sleep quality of hemodialysis patients, while zolpidem was better tolerated in these patients.
Cloning and expression of Mycobacterium bovis BCG DNA in "Streptomyces lividans"

The ability of "Streptomyces lividans" to use the expression signals of genes from Mycobacterium bovis BCG was tested in vivo by using gene fusions. Random DNA fragments from M. bovis BCG were inserted into promoter-probe plasmids in Escherichia coli and in "S. lividans. " Comparison with promoter activity detected with random DNA fragments from the respective hosts suggested that "S. lividans" efficiently utilizes a high proportion of mycobacterial promoters, whereas a smaller fraction are expressed, and expressed more weakly, in E. coli. M. bovis BCG DNA fragments were also inserted into the specially constructed translational fusion vector (pIJ688) in "S. lividans. " pIJ688 contains the kanamycin phosphotransferase gene (neo) from transposon Tn5, truncated at its amino terminus, as the indicator. The results suggested that "S. lividans" uses M. bovis BCG translational signals almost as efficiently as its own signals. Moreover, several hybrid proteins with an M. bovis BCG-derived amino terminus seemed to be reasonably stable in "S. lividans. " These experiments indicate that "S. lividans" may be a suitable host for the expression of Mycobacterium leprae and Mycobacterium tuberculosis genes from their own signals. This is a precondition for the expression of entire biosynthetic pathways, which could be valuable in the production of diagnostic and therapeutic agents. The vectors may also have wider applications for the analysis of gene expression in Streptomyces.
Monolayer Transition Metal Dichalcogenide Channel-Based Tunnel Transistor

We investigate the gate controlled direct band-to-band tunneling (BTBT) current in monolayer transition-metal dichalcogenide (MX<sub>2</sub>) channel-based tunnel field effect transistor (TFET). Five MX<sub>2</sub> materials (MoS<sub>2</sub>,MoSe<sub>2</sub>,MoTe<sub>2</sub>,WS<sub>2</sub>,WSe<sub>2</sub>) in their 2-D sheet forms are considered for this purpose. We first study the real and imaginary band structure of those MX<sub>2</sub> materials by density-functional theory (DFT), which is then used to evaluate the gate-controlled current under the Wentzel-Kramers-Brillouin (WKB) approximation. It is shown that all five MX<sub>2</sub> support direct BTBT in their monolayer sheet forms and offer an average ON current and subthreshold slope of 150 μA/μm (at V<sub>d</sub>=0.1 V) and 4 mV/dec, respectively. Furthermore, we also demonstrate the strain effect on the complex band structures and the performances of MX<sub>2</sub> based TFETs. It is observed that a certain tensile strain becomes favorable for the improvement of ON-current performances.
Simple Purification of Nicotiana benthamiana-Produced Recombinant Colicins: High-Yield Recovery of Purified Proteins with Minimum Alkaloid Content Supports the Suitability of the Host for Manufacturing Food Additives

Colicins are natural non-antibiotic bacterial proteins with a narrow spectrum but an extremely high antibacterial activity. These proteins are promising food additives for the control of major pathogenic Shiga toxin-producing E. coli serovars in meats and produce. In the USA, colicins produced in edible plants such as spinach and leafy beets have already been accepted by the U. S. Food and Drug Administration (FDA) and U. S. Department of Agriculture (USDA) as food-processing antibacterials through the GRAS (generally recognized as safe) regulatory review process. Nicotiana benthamiana, a wild relative of tobacco, N. tabacum, has become the preferred production host plant for manufacturing recombinant proteins—including biopharmaceuticals, vaccines, and biomaterials—but the purification procedures that have been employed thus far are highly complex and costly. We describe a simple and inexpensive purification method based on specific acidic extraction followed by one chromatography step. The method provides for a high recovery yield of purified colicins, as well as a drastic reduction of nicotine to levels that could enable the final products to be used on food. The described purification method allows production of the colicin products at a commercially viable cost of goods and might be broadly applicable to other cost-sensitive proteins.
Isotopic profiles imply strong convective influence on water near the tropical tropopause

The influence of deep convection on water vapor in the Tropical Tropopause Layer (TTL), the region just below the high ($\sim$18 km), cold tropical tropopause, remains an outstanding question in atmospheric science. Moisture transport to this region is important for climate projections because it drives the formation of local cirrus (ice) clouds, which have a disproportionate impact on the Earth's radiative balance. Deep cumulus towers carrying large volumes of ice are known to reach the TTL, but their importance to the water budget has been debated for several decades. We show here that profiles of the isotopic composition of water vapor can provide a quantitative estimate of the convective contribution to TTL moistening. Isotopic measurements from the ACE satellite instrument, in conjunction with ice loads inferred from CALIOP satellite measurements and simple mass-balance modeling, suggest that convection is the dominant source of water vapor in the TTL up to near-tropopause altitudes. The relatively large ice loads inferred from CALIOP satellite measurements can be produced only with significant water sources, and isotopic profiles imply that these sources are predominantly convective ice. Sublimating ice from deep convection appears to increase TTL cirrus by a factor of several over that expected if cirrus production were driven only by large-scale uplift; sensitivity analysis implies that these conclusions are robust for most physically reasonable assumptions. Changes in tropical deep convection in future warmer conditions may thus provide an important climate feedback.
Unexpected Protein Families Including Cell Defense Components Feature in the N-Myristoylome of a Higher Eukaryote*

N-Myristoylation is an irreversible modification that affects the membrane binding properties of crucial cytoplasmic proteins from signal transduction cascades. We characterized the two putative N-myristoyltransferases of Arabidopsis thaliana as a means of investigating the entire N-myristoylation proteome (N-myristoylome) in a higher eukaryote. AtNMT1 compensated for the nmt1 defect in yeast, whereas AtNMT2 and chimeras of the two genes did not. Only AtNMT1 modified known N-myristoylated proteins in vitro. AtNMT1 is therefore responsible for the A. thaliana N-myristoylome, whereas AtNMT2 does not seem to have usual myristoylation activity. We began with the whole set of N-myristoylated G proteins in the A. thaliana proteome. We then used a reiterative approach, based on the in vitro N-myristoylation of more than 60 different polypeptides, to determine the substrate specificity of AtNMT1. We found that the positive charge on residue 7 of the substrate was particularly important in substrate recognition. The A. thaliana N-myristoylome consists of 437 proteins, accounting for 1.7% of the complete proteome. We demonstrated the N-myristoylation of several unexpected protein families, including innate immunity proteins, thioredoxins, components of the protein degradation pathway, transcription factors, and a crucial regulatory enzyme of glycolysis. The role of N-myristoylation is discussed in each case; in particular, this process may underlie the “guard” hypothesis of innate immunity.