Miracle-dz/UniFAR-FTUs huggingface dataset:
CCAAT displacement protein, a regulator of differentiation-specific gene expression, binds a negative regulatory element within the 5' end of the human papillomavirus type 6 long control region

We have reported previously that a 636-bp fragment spanning the 5' two-thirds of the human papillomavirus type 6 (HPV6)-W50 long control region (LCR) functions as a transcriptional silencer (A. Farr, S. Pattison, B.-S. Youn, and A. Roman, J. Gen. Virol. 76:827-835, 1995). We have utilized nested deletion analyses to implicate a 66-bp sequence which appears to be critical for this activity. A comparison of the transcriptional regulatory activities of the LCRs of HPV6-W50 and HPV6b (which has a 94-bp deletion, resulting in the elimination of the 66-bp sequence) indicates that sequences within the 94-bp region negatively regulate the activity of the intact HPV6 LCR. Two sequence-specific DNA-protein interactions were visualized via electrophoretic mobility shift assays. One of the binding events is mediated by the transcriptional repressor CCAAT displacement protein (CDP), a factor which is active in undifferentiated cells but inactive in terminally differentiated cells. This conclusion is based on the following three lines of evidence: (i) a consensus CDP binding site oligonucleotide serves as a competitor in band shift assays, (ii) the band shift complex is not seen when a CDP-negative nuclear extract is used, and (iii) anti-CDP antiserum specifically inhibits the binding. These studies identify a DNA-protein interaction occurring within the 5' end of the LCR which may be important in maintaining the tight link between keratinocyte differentiation and HPV gene expression.
A monoclinic polymorph of (nitrato-κO)tetraphenylantimony(V)

The asymmetric unit of the title compound, [Sb(C6H5)4(NO3)], contains two crystallographically independent molecules. Each Sb atom exhibits a slightly distorted trigonal-bipyramidal geometry, with the O atom in the apical site. The crystal structure is stabilized by intermolecular C—H⋯O hydrogen bonds, forming a three-dimensional network.
A Novel UPLC-MS/MS Method for Determination of γ-Amino Butyric acid Analogue in Human Plasma: Application to Pharmacokinetic Study

Article history: Received on: 24/04/2013 Revised on: 16/05/2013 Accepted on: 05/05/2013 Available online: 27/06/2013 Pregabalin, a structural analogue of γ-amino butyric acid, is an anticonvulsant used for neuropathic pain. To facilitate its pharmacokinetic study in human subjects, ultra-performance liquid chromatography–mass spectrom etric method employing positive electrospray ionization was developed for the determination of Pregabalin concentration in human plasma. Pregabalin together with the internal standard was extracted from 125μl of human plasma by solid phase extraction. The chromatography was performed using an amide column and multiple reaction monitoring detection mode was used for the quantification of Pregabalin in plasma.The validation of the method including sensitivity, linearity, reproducibility and stability was examined.The lower limit of quantification (LLOQ) of the developed method for Pregabalin was 0.1 μg/mL and the linear calibration curve was acquired with r > 0.99 between 0.1 and 20 μg/ml. The intra-day and inter-day variation of the current assay was evaluated with the coefficient of variations within 10.73% at LLOQ and 9.8% for other quality control samples, whereas the mean accuracy ranged from 96.8% to 107.6%. The present method provides a robust, rapid and sensitive analytical tool for Pregabalin in human plasma and has been successfully applied to a clinical pharmacokinetic study where Pregabalin was orally administered in healthy subjects.
Factors influencing dry mouth in patients with primary Sjögren syndrome: usefulness of the ESSPRI index.

OBJECTIVE To evaluate health-related quality of life in a large series of primary SS patients using the recently-proposed ESSPRI questionnaire and to evaluate the relationship between the intensity of oral dryness and other signs and symptoms frequently found in these patients. METHODS We evaluated 90 primary SS patients seen consecutively; all fulfilled the current classification criteria. All patients completed the ESSPRI questionnaire. We compared the mean values of the ESSPRI-dry mouth item with other ESSPRI items related to sicca features, general symptoms, quality of life, quality of sleep, psychological and psychiatric features, extraglandular involvement, laboratory features and immunological markers and cardiovascular risk factors. Multivariate regression analysis with a backwards stepwise selection method was performed to identify those variables that were independently associated with dry mouth. RESULTS Mean intensity of oral dryness measured by the corresponding ESSPRI item was 7.17±0.23. Oral dryness correlated with age both at diagnosis and at study inclusion (p=0.013), but not with gender or with time of disease evolution. No significant correlation was found with the SF-36, HAQ and FIQ questionnaires. We found a significant correlation between the intensity of oral dryness and the quality of sleep (p=0.001), anxiety and depression measured by the GH28 (p=0.004 and 0.024, respectively), and a statistically-significant trend for anxiety and depression measured by the HADS (p=0.08 and 0.07, respectively). No significant correlation was found with the main extraglandular and immunological features; however, a significant correlation between oral dryness and hypertension (p=0.019), type II diabetes mellitus (p=0.005) and hypercholesterolemia (p=0.011) was found. Multivariate regression analysis shows that fatigue measured by ESSPRI (p=0.049), sleep quality (p=0.008) and hypercholesterolemia (p=0.008) were independently associated with dry mouth. CONCLUSION We report on the usefulness of the ESSPRI index in evaluating HRQOL associated with oral dryness in primary SS patients. Oral dryness correlated with age and the other sicca symptoms measured by ESSPRI, but not with the main systemic and immunological SS features. In contrast, oral dryness was strongly correlated with fatigue, pain, psychological distress, poor sleep and vascular risk factors. A multidisciplinary therapeutic approach may be the best way of minimizing oral dryness and its consequences in primary SS patients.
Acute portal vein thrombosis at the onset of a nephrotic syndrome.

Thromboembolic events are common but serious complications of the nephrotic syndrome. Even if multiple studies failed to demonstrate a direct link between occurrence of these complications and haemostatic abnormalities, the nephrotic syndrome is characterized by a hypercoagulable state resulting to various extent from (i) thrombocytosis and platelet hyperaggregability, (ii) increased plasma levels of factors V and VIII, and of fibrinogen, (iii) decreased plasma levels of antithrombin III, free protein S, and protein C, and (iv) alterations in the fibrinolytic system [1-3]. The most common sites of thrombosis during adult nephrotic syndrome are deep veins of the lower limbs and renal veins, the latter being especially involved in membranous nephropathy. Morphological studies with Doppler ultrasonography and ventilation/perfusion isotope scanning reveal a higher incidence of thrombotic complications than expected from clinical studies with numerous patients being symptomless [4]. Arterial thrombosis affecting mesenteric, axillary, femoral, ophthalmic, renal, pulmonary, and coronary arteries has also been reported, but mainly in children. We describe here a case of portal vein thrombosis that occurred 9 days after the onset of a nephrotic syndrome in an adult patient. Only two cases of this unusual thrombotic complication of the adult nephrotic syndrome have been reported [5,6], and then only in relapsing patients.
An ECoG Based Brain Computer Interface with Spatially Adapted Time-Frequency Patterns

In this paper we describe an adaptive approach for the classification of multichannel electrocorticogram (ECoG) recordings for a Brain Computer Interface. In particular the proposed approach implements a timefrequency plane feature extraction strategy from multichannel ECoG signals by using a dual-tree undecimated wavelet packet transform. The dual-tree undecimated wavelet packet transform generates a redundant feature dictionary with different time-frequency resolutions. Rather than evaluating the individual discrimination performance of each electrode or candidate feature, the proposed approach implements a wrapper strategy to select a subset of features from the redundant structured dictionary by evaluating the classification performance of their combination. This enables the algorithm to optimally select the most informative features coming from different cortical areas and/or time frequency locations. We show experimental classification results on the ECoG data set of BCI competition 2005. The proposed approach achieved a classification accuracy of 93% by using only three features.
Prevalence of food borne pathogens in market samples of chicken meat in Bangalore.

A study on the prevalence of common food borne pathogens (Salmonella, Staphylococcus and E. Coli) in chicken meat obtained from wet market in Bangalore under different processing conditions was carried out. Results revealed higher prevalence of Salmonella in the range of 25 to 65 per cent and the level of contamination of meat with Salmonella decreased with increase in sophistication of slaughter facility and that thigh muscle were highly prone for contamination compared to the breast muscle irrespective of the processing condition. Cent percent prevalence of Staphylococcus was observed in outlets with minimum facilities compared to those meat obtained from outlets with better facilities and hygiene. The prevalence of E. Coli followed a similar trend with a range between 42 to 88 per cent indicating the lower hygiene of the meat obtained from the wet market. Lack of hygiene and better facilities have been major cause of poor microbial quality of meat.
Iodine‐123 labeled reboxetine analogues for imaging of noradrenaline transporter in brain using single photon emission computed tomography

Preliminary investigation of the radioiodinated (S,S)‐reboxetine analogue, 123I‐INER, in baboons showed this tracer to have promise for imaging the noradrenaline transporter (NAT) using single photon emission computed tomography (SPECT). More recently, the radioiodinated (R,S)‐stereoisomer of 123I‐INER, 123I‐NKJ64, has been synthesized and preliminary evaluation in rats has been reported. This article reports the brain distribution and pharmacokinetic properties of 123I‐NKJ64 in baboons and compares results with 123I‐INER data in the same species. SPECT studies were conducted in two ovariectomized adult female baboons using two different protocols: (1) bolus of 123I‐INER or 123I‐NKJ64; and (2) bolus plus constant infusion of 123I‐NKJ64 with reboxetine (2.0 mg/kg) administration at equilibrium. Following bolus injection, both radiotracers rapidly and avidly entered the baboon brain. The regional brain accumulation of 123I‐NKJ64 did not match the known distribution of NAT in baboon brain, contrasting with previous results obtained in rats. Conversely, the regional distribution of 123I‐INER was consistent with known distribution of NAT in baboon brain. No displacement of 123I‐NKJ64 was observed following administration of reboxetine. This contrasts with previous data obtained for 123I‐INER, where 60% of specific binding was displaced by a lower dose of reboxetine. These data suggest that 123I‐NKJ64 may lack affinity and selectivity for NAT in baboon brain and 123I‐INER is the most promising iodinated reboxetine analogue developed to date for in vivo imaging of NAT in brain using SPECT. This study highlights the importance of species differences during radiotracer development and the stereochemical configuration of analogues of reboxetine in vivo. Synapse 66:923–930, 2012. © 2012 Wiley Periodicals, Inc.
The relationship between executive functions and IQ in Korean children and the comparison with Chinese children

ABSTRACT This study aimed to investigate the relationship between perceived/performance-based executive function and IQ. Additionally, the relationship between perceived executive function and intelligence was investigated cross-culturally between South Korea and China. Korean children (60; M = 34, F = 26, Mean age = 10.35) were included in study 1, and Korean children (43, M = 23, F = 20, Mean age = 10.05) and Chinese children (56; M = 29, F = 27, Mean age = 10.40) were included in study 2. The Korean-Wechsler Intelligence Scale for Children-IV, the Stroop test, the CTT-2, and the executive function questionnaire were used for Korean subjects, and the Raven's matrix test and the executive function questionnaire were used for Korean and Chinese subjects. Multiple regression showed that CTT-2(RT), emotional control difficulty, and Color Word within a 45’ Stroop test trial were significant predictors of total IQ. The cross-cultural analysis showed a statistically significant difference between the two countries in the emotional control aspect of perceived executive function. There were no interactions between country and intelligence. In conclusion, intelligence was related to overall executive function. Korean children and Chinese children showed cultural differences in processing emotion. These results are expected to contribute to developing therapeutic strategies for executive function in children and to exchanging these strategies between Korea and China.
An Improved Fault-Tolerant Five-Phase Induction Machine Using a Combined Star/Pentagon Single Layer Stator Winding Connection

One of the main merits offered by multiphase machines is their high fault-tolerant capability. Literature has demonstrated that the performance of multiphase induction machines under fault conditions is affected by the employed stator winding connection. In open-loop controlled five-phase machines, the star connection is favorable under healthy conditions while the pentagon connection is favorable and yields a lower derating factor under the open phase condition. In this paper, a new combined star/pentagon single layer winding layout that combines the advantages of both star and pentagon connections is proposed for a five-phase induction machine. Although the proposed winding is intrinsically an asymmetrical ten-phase machine, the proposed connection allows for only five-phase terminals. Moreover, the proposed winding not only yields better flux distribution compared to a conventional single layer winding, but also provides a complete cancellation of the third-order harmonic flux component caused by the induced third sequence currents due to the saturation effect and/or under unbalanced operation. Hence, the machine losses are decreased, which improves the overall machine efficiency. For the healthy case, the machine is similar to a conventional star connected five-phase machine. However, with one phase open, the proposed connection results in a lower derating factor compared to conventional connections for both open-loop and optimal current control techniques. A 1-kW prototype machine is used for experimental verification.
Disruptive Behaviors in the Medical Setting and Borderline Personality

Objective: Borderline personality disorder (BPD) is characterized in the DSM by anger. While previous studies have examined various types of externalized aggression and violence among individuals with BPD, we are aware of no investigations of disruptive behaviors perpetrated by patients with BPD in medical settings—the focus of the present study. Method: Using a cross-sectional, consecutive sample of internal medicine outpatients from a resident provider clinic, we surveyed 397 participants regarding 17 disruptive behaviors related to medical personnel and assessed BPD using two self-report measures, the BPD scale of the Personality Diagnostic Questionnaire-4 (PDQ-4) and the Self-Harm Inventory (SHI). Results: A statistically significantly greater proportion of respondents who exceeded the clinical cut-off scores for BPD on both the PDQ-4 and SHI endorsed at least one form of disruptive behavior. However, there were no differences between participants with versus without BPD symptomatology regarding the number of different forms of disruptive behaviors after that initial threshold of one such behavior was reached. Several specific disruptive behaviors were reported more often in those with BPD symptomatology (i.e., yelling, screaming, verbally threatening, and refusing to talk with medical staff as well as talking badly about medical staff to the patients' friends and family). Conclusions: BPD appears to be associated with a greater likelihood of disruptive behaviors in the medical setting, but not physical threat.
Differential expression of two plant-like enolases with distinct enzymatic and antigenic properties during stage conversion of the protozoan parasite Toxoplasma gondii.

The precise molecular mechanisms underlying the switch between the two developmental stages of Toxoplasma gondii, and the metabolic adaptations occurring during this stage conversion are poorly understood. Because inhibitors of mitochondrial respiration are known to trigger differentiation from tachyzoite into bradyzoite stages, we believe that some of the switch components may be sought in the regulation of central carbohydrate metabolism. We have previously described a cDNA encoding a bradyzoite-specific enolase, ENO1. We now report the isolation and characterization of another enolase-encoding cDNA (ENO2) that is expressed preferentially in the tachyzoite stage. The deduced amino acid sequences of ENO1 and ENO2 share 73.65 % identity. They both display significant homologies to plant enolases with the presence of two plant-like peptide insertions, a pentapeptide EWGW(Y)C(S) and a dipeptide EK (or DK). We demonstrate that deletions of the ENO1 pentapeptide motif on its own or together with the dipeptide reduce drastically the affinity for the 2PGA substrate, suggesting that the evolutionary acquisition of these peptides in enolases of land plants and apicomplexan parasites contribute a specific function to their enzymatic activities. T. gondii ENO1 and ENO2 were also expressed as active recombinant enzymes in Escherichia coli. While ENO1 and ENO2 display similar K(m) values, the pure tachyzoite-specific enzyme (ENO2) has a threefold specific activity at V(max) compared with that of the bradyzoite-specific enolase (ENO1). Moreover, immunoblot analyses performed using polyclonal antibodies raised against the recombinant enzymes revealed that the native enolase in tachyzoite and bradyzoite are also antigenically distinct. Taken together, our results indicate that the differences witnessed between the two activities may be instrumental in maintaining glycolysis in pace with the distinct stage-specific requirements of carbohydrate metabolism.
Transit Assignment for Congested Public Transport Systems: An Equilibrium Model

We propose a new formulation for the assignment problem over congested transit networks. The congestion effects due to insufficient capacity of system elements transit lines are considered to be concentrated at transit stops. Waiting times on access links are therefore dependent on passenger flows. A special formulation of the transit network is used in order to model correctly the congestion effects. Finally, algorithms for solution are analyzed.
Expression of the MHC Class II Pathway in Triple-Negative Breast Cancer Tumor Cells Is Associated with a Good Prognosis and Infiltrating Lymphocytes

The MHC II pathway is usually turned off in tumor cells. Expression in triple-negative breast tumors was correlated with antitumor responses and reduced relapse risk. MHC II expression may predict good prognosis, and inducing it may have therapeutic benefits. Triple-negative breast cancer (TNBC) is a subtype with heterogeneous patient outcomes. Approximately 40% of patients experience rapid relapse, while the remaining patients have long-term disease-free survival. To determine if there are molecular differences between primary tumors that predict prognosis, we performed RNA-seq on 47 macrodissected tumors from newly diagnosed patients with TNBC (n = 47; 22 relapse, 25 no relapse; follow-up median, 8 years; range, 2–11 years). We discovered that expression of the MHC class II (MHC II) antigen presentation pathway in tumor tissue was the most significant pathway associated with progression-free survival (HR, 0.36; log-rank P = 0.0098). The association between MHC II pathway expression and good prognosis was confirmed in a public gene expression database of 199 TNBC cases (HR, 0.28; log-rank P = 4.5 × 10–8). Further analysis of immunohistochemistry, laser-capture microdissected tumors, and TNBC cell lines demonstrated that tumor cells, in addition to immune cells, aberrantly express the MHC II pathway. MHC II pathway expression was also associated with B-cell and T-cell infiltration in the tumor. Together, these data support the model that aberrant expression of the MHC II pathway in TNBC tumor cells may trigger an antitumor immune response that reduces the rate of relapse and enhances progression-free survival. Cancer Immunol Res; 4(5); 390–9. ©2016 AACR.
Psychological and marital adjustment in couples following a traumatic brain injury (TBI): A critical review

The first part of this paper examines current data describing the psychological and marital adjustment of couples following a traumatic brain injury (TBI). Although these findings reveal some discrepancies, they highlight that adjustment following a TBI represents a genuine challenge for those involved in the process. The second part moves toward the examination of factors associated with psychological and marital adjustment in both couple partners. Here again, there exists a large diversity in empirical data and theoretical models informing this emerging area of interest. Nevertheless, cognitive variables such as coping skills are commonly seen as critical variables to explain the adjustment level in people with TBI and their spouse/caregivers. Concurrently with the discussion of the methodological issues and pitfalls encountered in this area of research, the conclusion provides suggestions of further steps to undertake in this endeavour toward a better understanding of the adjustment process following TBI.
Circadian temperature and melatonin rhythms , sleep , and neurobehavioral function in humans living on a 20h day

Wyatt, James K. , Angela Ritz-De Cecco, Charles A. Czeisler, and Derk-Jan Dijk. Circadian temperature and melatonin rhythms, sleep, and neurobehavioral function in humans living on a 20-h day. Am. J. Physiol. 277 (Regulatory Integrative Comp. Physiol. 46): R1152–R1163, 1999. —The interaction of homeostatic and circadian processes in the regulation of waking neurobehavioral functions and sleep was studied in six healthy young subjects. Subjects were scheduled to 15–24 repetitions of a 20-h rest/activity cycle, resulting in desynchrony between the sleep-wake cycle and the circadian rhythms of body temperature and melatonin. The circadian components of cognitive throughput, shortterm memory, alertness, psychomotor vigilance, and sleep disruption were at peak levels near the temperature maximum, shortly before melatonin secretion onset. These measures exhibited their circadian nadir at or shortly after the temperature minimum, which in turn was shortly after the melatonin maximum. Neurobehavioral measures showed impairment toward the end of the 13-h 20-min scheduled wake episodes. This wake-dependent deterioration of neurobehavioral functions can be offset by the circadian drive for wakefulness, which peaks in the latter half of the habitual waking day during entrainment. The data demonstrate the exquisite sensitivity of many neurobehavioral functions to circadian phase and the accumulation of homeostatic drive for sleep.