Miracle-dz/UniFAR-FTUs huggingface dataset:
Characteristics of lower airway inflammatory changes in the minimal persistent inflammation of allergic rhinitis in mice

ABSTRACT Objective: This study aims to establish an experimental mouse model of minimal persistent inflammation (MPI), observe the features of inflammation and hyper-responsiveness of the upper/lower airways, and explore the relationship between inflammation and hyper-responsiveness in the upper/lower airways. Methods: Sixty-four female BALB/c mice were randomly divided into four groups: allergic rhinitis (AR) group as positive control, MPI group, negative control group and blank control group. Mice were given high and low-concentrated ovalbumin solution after basic and intensive sensitization to establish AR model and MPI model. Nasal mucosa and lung tissues were stained to observe eosinophil infiltration, goblet cell hyperplasia, and expression of intercellular adhesion molecule 1 (ICAM-1). Airway hyper-responsiveness was assessed. Levels of specific immunoglobulin E (sIgE), interleukin (IL)-4 and IL-5 in peripheral blood, nasal lavage fluid (NLF), and bronchoalveolar lavage fluid (BALF) were detected by Enzyme-linked immunosorbent assay. Results: The eosinophil infiltration and expression of ICAM-1 on nasal mucosa and in lung tissues in the AR and MPI groups were significantly elevated compared to control groups. Goblet cells count increased only in the nasal mucosa and not in lung tissues. Eosinophil and neutrophil count of NLF and BALF in the AR and MPI groups increased significantly compared to control groups. Level of IL-4 did not increase significantly, but sIgE and IL-5 did. Conclusions: Mice in the MPI status exhibits lower airway inflammation and hyper-responsiveness with increase in eosinophil count, goblet cells, ICAM-1, IL-4, and IL-5. These results provide further evidence for the importance of MPI of AR in lower airway diseases.
A Role for MeCP 2 in Switching Gene Activity via Chromatin Unfolding and HP 1 gamma Displacement

Methyl-CpG-binding protein 2 (MeCP2) is generally considered to act as a transcriptional repressor, whereas recent studies suggest that MeCP2 is also involved in transcription activation. To gain insight into this dual function of MeCP2, we assessed the impact of MeCP2 on higher-order chromatin structure in living cells using mammalian cell systems harbouring a lactose operator and reporter gene-containing chromosomal domain to assess the effect of lactose repressor-tagged MeCP2 (and separate MeCP2 domains) binding in living cells. Our data reveal that targeted binding of MeCP2 elicits extensive chromatin unfolding. MeCP2-induced chromatin unfolding is triggered independently of the methyl-cytosine-binding domain. Interestingly, MeCP2 binding triggers the loss of HP1c at the chromosomal domain and an increased HP1c mobility, which is not observed for HP1a and HP1b. Surprisingly, MeCP2-induced chromatin unfolding is not associated with transcriptional activation. Our study suggests a novel role for MeCP2 in reorganizing chromatin to facilitate a switch in gene activity. Citation: Brink MC, Piebes DGE, de Groote ML, Luijsterburg MS, Casas-Delucchi CS, et al. (2013) A Role for MeCP2 in Switching Gene Activity via Chromatin Unfolding and HP1c Displacement. PLoS ONE 8(7): e69347. doi:10.1371/journal.pone. 0069347 Editor: Nicoletta Landsberger, University of Insubria, Italy Received February 8, 2013; Accepted June 7, 2013; Published July 2 , 2013 Copyright: 2013 Brink et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: Support was provided by the Netherlands Organization for Scientific Research VIDI-016041311, NWO-Meervoud-83607001 to PJV, VIDI-91786373 to MGR and Deutsche Forschungsgemeinschaft to MCC. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interest exist. * E-mail: P.J.Verschure@uva.nl . These authors contributed equally to this work. ¤ Current address: Department of Toxicogenetics, Leiden University Medical Center, Leiden, the Netherlands
The effect of soil‐particle size on hydrocarbon entrapment near a dynamic water table

Abstract The entrapment of residual hydrocarbon globules by water table fluctuations can produce a long‐term contamination threat to groundwater supplies that is difficult to remove. The mobilization of entrapped hydrocarbon globules depends on the balance between capillary and gravitational forces represented by the Bond number. It is important to estimate the potential for hydrocarbon entrapment at a spill site due to its influence on the effectiveness of remediation efforts. The present work focuses on the influence of particle diameter on hydrocarbon entrapment for a typical LNAPL (light nonaqueous‐phase liquid). Laboratory column tests have been conducted using a dual‐beam gamma densitometer to measure saturations of the three phases (water, air, and hydrocarbon). Soltrol 170®, a solvent manufactured by Phillips 66 Co., is used as the hydrocarbon. Residual saturation of the Soltrol is measured after fluctuations in water table level to establish the distribution and consistency of hydrocarbon entrapm.
Corrigendum to “Evacuation after the Fukushima Daiichi Nuclear Power Plant Accident Is a Cause of Diabetes: Results from the Fukushima Health Management Survey”

The Great East Japan Earthquake and Fukushima Daiichi nuclear disaster in 2011 forced the evacuation of a large number of residents and created changes in the lifestyle of the evacuees. These changes may have affected the evacuees' glucose metabolism, thereby leading to an increase in the incidence of diabetes. This study included Japanese men and women who were living near the Fukushima Daiichi Nuclear Power Plant in Fukushima prefecture before the disaster. Subjects subsequently underwent annual health checkups with a focus on metabolic syndromes, which were conducted under the Health Care Insurers. Using the Comprehensive Health Check survey, we analyzed changes in the glucose metabolism before and after the disaster. A total of 27,486 subjects underwent follow-up examinations after the disaster, with a mean follow-up period of 1.6 years. After the disaster, the prevalence of diabetes increased significantly, and we observed that the incidence of diabetes was significantly greater among evacuees than among nonevacuees. Furthermore, multivariate logistic regression analysis revealed that evacuation was significantly associated with the incidence of diabetes. In conclusion, this is the first study to demonstrate that evacuation is associated with the incidence of diabetes. This information may be used to guide follow-up recommendations for evacuees.
Learning in adaptive spaces:how customer experience professionals experience learning during technology-mediated interaction, and implications for organisational learning

This research investigates how customer experience (CX) professionals experience learning through their use of digital technologies in organisations, and considers the implications for organisational learning. A phenomenographic methodology was used to compare the variation in employees’ experiences of learning, and the research employed a conceptual framework of post-structuralism and complexity to investigate how digital technologies affect organisational learning and knowledge management. Complexity Leadership Theory was used as a way to interpret the complexity dynamics that occur through digitally mediated interactions in organisations, and provided a way to conceptualise these interactions as taking place in ‘adaptive spaces’. The research found that a lack of etiquette regarding the use of digital tools can adversely affect processes of meaning-creation during the technology-mediated work of CX professionals. The findings indicate that a more intentional use of technology – a ‘digital etiquette’ – can be viewed as a dynamic capability, and has the potential to improve the way in which CX professionals contribute to organisational learning. The findings also demonstrate that improving digital etiquette in adaptive spaces is an appropriate response to problems of knowledge management under conditions of complexity. The research will be of interest to those seeking a clearer understanding of the potential of the CX function to contribute to organisational learning, and also to those aiming to design programmes of learning that prepare students effectively for complex environments.
The motion of a falling liquid filament

When a liquid drop falls from a fluid source with a slow flow rate, it remains attached to the source by an elongating liquid filament until the filament pinches off. For many fluids, this pinch-off occurs first near the end of the filament, where the filament joins to the liquid drop. For other fluids, the filament pinches off at one or more interior points. In this paper, we study the motion of this filament, and we make two points. First, the flow in this filament is not that of a uniform jet. Instead, we show experimentally that a different solution of the Navier–Stokes equations describes the motion of this filament before it pinches off. Second, we propose a criterion for the location of the first pinch-off. In particular, we analyze the linearized stability of the exact solution, both for an inviscid fluid and for a very viscous fluid. Our criterion for pinch-off is based on this stability analysis. It correctly predicts whether a given filament pinches off first near its ends or at points within i.
Linking clinical care to community resources for cardiovascular disease prevention: the North Carolina Enhanced WISEWOMAN project.

BACKGROUND The Well-Integrated Screening and Evaluation for Women Across the Nation (WISEWOMAN) project addresses the need for effective cardiovascular disease (CVD) prevention among underserved, midlife women. We describe an Enhanced Intervention that addressed environmental and individual factors within the context of a clinic-based intervention. We also present the study design and baseline results of the randomized trial to evaluate the enhanced intervention. METHODS The multicomponent behavior change intervention addressed many elements of the Chronic Care Model (CCM), including the community resources and policy element, wherein Enhanced Intervention participants were encouraged to overcome environmental barriers to a healthy lifestyle by using community resources. Study participants were enrolled at one community health center; all were low-income, underinsured, midlife (40-64 years) women. RESULTS A total of 236 participants were randomized to receive the Enhanced Intervention or the Minimum Intervention. At baseline, over three fourths of the participants were overweight or obese. Participants reported a variety of problematic neighborhood characteristics, including a paucity of restaurants with healthy food choices (41% reported as a problem); not enough farmer's markets or produce stands (50%), not enough affordable exercise places (52%), not enough physical activity programs that met women's needs (42%), heavy traffic (47%), and speeding drivers (53%). Overall, women knew little about affordable exercise venues and nutrition classes. CONCLUSIONS In this clinic-based intervention, we addressed environmental factors related to a healthy lifestyle. Results indicate the need for effective and feasible intervention strategies to address the environments in which individuals are making behavior changes. The effectiveness of the WISEWOMAN Enhanced Intervention will be assessed in a randomized trial.
Perceptions and attitudes of modern and traditional medical practitioners about traditional medical practice in Eritrea

In Eritrea1.traditional medicine has long roots and it has been in existence even before the advent of modern medicine. It is still widely used in prevention and treatment of physical and mental disorders as well as social imbalance. In Eritrea, the traditional medical system includes herbalists, traditional birth attendants (TBAs), spiritual healers, druggist, magicians, bone-settlers etc. Traditional medicine is the sum total of knowledge or practices whether explicable or inexplicable, used in diagnosing, preventing or eliminating a physical, mental or social disease which may rely exclusively on past experience or observations handed down from generation to generation, verbally or in writing [2]. It also comprises therapeutic practices that have been in existence often for hundreds of years before the development of modern scientific medicine and are still in use today without any documented evidence of adverse effects. Volume 11 Issue 1 2018
Large eddy simulation of ship tracks in the collapsed marine boundary layer: a case study from the Monterey area ship track experiment

Abstract. For the first time, a large eddy simulation (LES) coupled to a bulk aerosol scheme is used to simulate an aircraft-sampled ship track. The track was formed by the M/V Sanko Peace on 13 June 1994 in a shallow drizzling boundary layer with high winds but very low background aerosol concentrations (10 cm −3 ). A Lagrangian framework is used to simulate the evolution of a short segment of track as it is advected away from the ship for 8 h (a downwind distance exceeding 570 km). Using aircraft observations for initialization, good agreement is obtained between the simulated and observed features of the ambient boundary layer outside the track, including the organization of the cloud into mesoscale rolls. After 8 h, a line of aerosol is injected to start the ship track. The simulation successfully reproduces the significant albedo enhancement and suppression of drizzle observed within the track. The aerosol concentration within the track dilutes as it broadens due to turbulent mixing. A sensitivity study shows the broadening rate strongly depends on the alignment between the track and the wind-aligned boundary layer rolls, as satellite images of ship tracks suggest. Entrainment is enhanced within the simulated track, but the observed 100 m elevation of the ship track above the surrounding layer is not simulated, possibly because the LES quickly sharpens the rather weak observed inversion. Liquid water path within the simulated track increases with time even as the ambient liquid water path is decreasing. The albedo increase in the track from liquid water and cloud fraction enhancement (second indirect effect) eventually exceeds that from cloud droplet number increases (first indirect or Twomey effect). In a sensitivity study with a higher initial ambient aerosol concentration, stronger ship track aerosol source, and much weaker drizzle, there is less liquid water inside the track than outside for several hours downwind, consistent with satellite estimates for such situations. In that case, the Twomey effect dominates throughout, although, as seen in satellite images, the albedo enhancement of the track is much smaller.
On the dark side of work: a longitudinal analysis using self-determination theory

ABSTRACT As the nature of work has changed in recent decades, employees are increasingly exposed to psychological demands in the workplace, which have associated consequences for employees, organizations, and society. Using self-determination theory, this study examined the dark side of work, in which frustration of basic psychological needs is associated with higher levels of work-related stress. In this model, work-related stress is associated with higher levels of somatic symptom burden, which in turn is associated with higher levels of emotional exhaustion, turnover intention, and absenteeism. Results of a longitudinal analysis using data from four time points over 15 months supported these predictions. Taken together, this study advances the literature towards an understanding of the (potential) detrimental impact that need-thwarting work contexts can have on employee wellness and work-related outcomes.
Preliminary FISH-Based Assessment of External Dose for Residents Exposed on the Techa River

This paper presents the results of a feasibility cytogenetic study using the fluorescence in situ hybridization (FISH) translocation assay for residents of villages located on the Techa River (Southern Urals, Russia) contaminated with liquid radioactive wastes from the Mayak plutonium facility in 1949–1956. The study was conducted with two groups of donors that differed in their main pathways of exposure. The first group comprised 18 residents of the middle Techa region who were exposed predominantly from ingestion of radionuclides (mostly 89,90Sr) via the river water and local foodstuffs. The second group included 20 residents of Metlino, the closest village to the site of releases, who were exposed to external γ radiation from the contaminated river bank and exposed internally from dietary intake of radionuclides. A significant linear dependence between the radiation-induced translocation frequency and individual red bone marrow dose from incorporated 89,90Sr, calculated with the Techa River Dosimetry System (TRDS), was found in the first group of donors. This allowed us to take the contribution of 89,90Sr to the total radiation-induced translocation frequency into account for the second group of donors and to analyze translocations resulting from external γ-ray exposure. Individual doses from external exposure derived from the corrected translocation frequency for the second group of donors (Metlino residents), using a linear dose–response coefficient of 0.015 translocation/cell/Gy recommended by Edwards et al. in 2005, were shown to vary up to 2.1 Gy, with an average value of 0.48 Gy, which was in agreement with TRDS-based external dose estimates for Metlino residents.
An investigation into the relationship between customer relationship marketing and customer retention: superstore retailing context in Bangladesh

The context of this study is Bangladesh`s food retailing sector. The main purpose of this study is to investigate the relationship between Customer Relationship Marketing (CRM) and customer retention. The core aim of Relationship Marketing is to build long lasting mutually bonded relationships with customers and various other important stakeholders. The concept has attracted considerable attention among scholars in recent decades and has appeared in service marketing literature as a new marketing paradigm. The concept is critical to the success of any organisation as it has been an accepted phenomenon that maintains that existing customers are far easier to retain than is the process of acquiring new customers. In order to stay in business and cope with the challenging business dynamism, organisations are continuously searching for reliable and serviceable strategies to be employed in order to increase customer retention. However, there is a lack of consensus among researchers on the core antecedents of relationship marketing that can be used to achieve the above aims, especially while the concept is new in the context of organised retailing sectors in Bangladesh. In response, the study developed a conceptual framework of customer retention strategy which incorporates bonds, service quality and relational quality into one relationship model. The model establishes eleven hypotheses. A sample of 202 grocery food retail customers were selected in a random sample from four selected superstores. The results support hypothesized relationships built on the model. The findings indicate that service quality, trust, bond and customer satisfactions are vital for creating positive customer loyalty which in turn creates customer retention. Corresponding author: Palto Datta Email addresses for corresponding author: paltodatta@yahoo.co.uk First submission received: 10th August 2017 Revised submission received: 24th May 2018 Accepted: 9th July 2018
Hemodynamic effects of lactotripeptides from casein hydrolysate in Mediterranean normotensive subjects and patients with high-normal blood pressure: a randomized, double-blind, crossover clinical trial.

Contrasting data partially support a certain antihypertensive efficacy of lactotripeptides derived from enzymatic treatment of casein hydrolysate. We carried out a randomized, double-blind, crossover clinical study to investigate the antihypertensive efficacy of a short-term treatment with lactotripeptides in Mediterranean subjects with normal or high-normal blood pressure (BP). We consecutively enrolled 55 untreated subjects (men:women = 30:25), 40.3 ± 9.8 years old, with normal or high-normal BP. After 4 weeks of dietary standardization, they were allocated to treatment with a fruit juice containing 3 mg of added Ile-Pro-Pro/Val-Pro-Pro lactotripeptides or with placebo for 4 weeks. After a 4-week washout period, they were then assigned to the alternative treatment for a further period of 4 weeks. Overall, no significant difference has been observed in office BP comparing baseline data with those posttreatment. Repeating the analysis by basal BP level, a mild but significant reduction in systolic BP (-1.7 ± 2.3 mm Hg; t = 3.5, P = .002) has been observed only in subjects with high-normal BP after treatment with lactotripeptides. With regard to 24-hour BP measurement, after lactotripeptide treatment only, the subjects experienced a significant reduction in diurnal diastolic BP (-1.6 ± 5.4 mm Hg; P = .042), diurnal mean BP (-2.1 ± 5.9 mm Hg; P = .19), and 24-hour (-5.4 ± 14.2 mm Hg; P = .011) and diurnal (-7.1 ± 19.2%; P = .014) diastolic BP value measurements relative to normal values. No modification has been observed in relation to plasma renin activity and aldosteronemia. In conclusion, diurnal diastolic BP is significantly reduced by lactrotripeptide supplementation in untreated Mediterranean subjects with normal or high-normal BP. Office systolic BP is reduced only in subjects with high-normal BP.
Hepatocyte Nuclear Factor 1 Regulates the Expression of the Organic Cation Transporter 1 via Binding to an Evolutionary Conserved Region in Intron 1 of the OCT 1 Gene s

The organic cation transporter 1 (OCT1), also known as solute carrier family 22 member 1, is strongly and specifically expressed in the human liver. Here we show that the hepatocyte nuclear factor 1 (HNF1) regulates OCT1 transcription and contributes to the strong, liver-specific expression of OCT1. Bioinformatic analyses revealed strong conservation of HNF1 binding motifs in an evolutionary conserved region (ECR) in intron 1 of the OCT1 gene. Electrophoretic mobility shift and chromatin immunoprecipitation assays confirmed the specific binding of HNF1 to the intron 1 ECR. In reporter gene assays performed in HepG2 cells, the intron 1 ECR increased SV40 promoter activity by 22-fold and OCT1 promoter activity by 13-fold. The increase was reversed when the HNF1 binding sites in the intron 1 ECR were mutated or the endogenous HNF1a expression was downregulated with small interfering RNA. Following HNF1a overexpression in Huh7 cells, the intron 1 ECR increased SV40 promoter activity by 11fold and OCT1 promoter activity by 6-fold. Without HNF1a overexpression, the increases were only 3and 2-fold, respectively. Finally, in human liver samples, high HNF1 expression was significantly correlated with high OCT1 expression (r5 0.48, P5 0.002, n 5 40). In conclusion, HNF1 is a strong regulator of OCT1 expression. It remains to be determined whether genetic variants, disease conditions, or drugs that affect HNF1 activity may affect the pharmacokinetics and efficacy of OCT1-transported drugs such as morphine, tropisetron, ondansetron, tramadol, and metformin. Beyond OCT1, this study demonstrates the validity and usefulness of interspecies comparisons in the discovery of functionally relevant genomic sequences.