Miracle-dz/UniFAR-FTUs huggingface dataset:
Maternal Stress During Pregnancy Is Associated with Decreased Cortisol and Cortisone Levels in Neonatal Hair

Background: Hair glucocorticoids (GCs) offer a retrospective view on chronic GC exposure. We assessed whether maternal pre- and postnatal stress was associated with neonatal and maternal hair GCs postpartum (pp). Methods: On the first day pp 172 mother-infant pairs donated hair, of whom 67 had consulted a centre of expertise for psychiatric disorders during pregnancy. Maternal stress was scored on the Hospital Anxiety and Depression Scale during the first/second (n = 46), third trimester (n = 57), and pp (n = 172). Hair cortisol and cortisone levels were determined by liquid chromatography-tandem mass spectrometry, and associations with maternal hospital anxiety subscale (HAS) and hospital depression subscale (HDS) scores, and antidepressant use were analyzed with linear regression. Results: Neonatal hair GCs were negatively associated with elevated HAS-scores during the first/second trimester, log 10 (β [95% CI]) cortisol –0.19 (–0.39 to 0.02) p = 0.07, cortisone –0.10 (–0.25 to 0.05) p = 0.17; third trimester, cortisol –0.17 (–0.33 to 0.00) p = 0.05, cortisone –0.17 (–0.28 to –0.05) p = 0.01; and pp, cortisol –0.14 (–0.25 to –0.02) p = 0.02, cortisone –0.07 (–0.16 to 0.02) p = 0.10. A similar pattern was observed for elevated HDS-scores. Maternal hair GCs were positively associated with elevated HAS-scores pp (cortisol 0.17 [0.01 to 0.32] p = 0.04, cortisone 0.18 [0.06 to 0.31] p = 0.01), but not prenatally or with elevated HDS-scores. Antidepressant use was associated with elevated maternal hair GCs (p ≤ 0.05), but not with neonatal hair GCs. Conclusion: Exposure to excessive pre- and perinatal maternal stress was associated with a decrease in neonatal hair GCs, while elevated stress-scores around birth were associated with increased maternal hair GCs and elevated stress-scores earlier in gestation were not associated with maternal hair GCs pp. Further studies are needed to test associations with infant neurodevelopment.
The effect of Honey-Spots on the Consumers' Responsiveness towards Mobile Advertisement

This paper aims to investigate the impact of context on the consumers’ responsiveness towards Location-Based Advertisement (LBA) on mobile devices. Based on existing theories and models, we show in a real-life scenario that the receiving context is crucial for the effectiveness of LBA. Our scenario promotes the idea of choosing a more distant location with a more suitable receiv-ing context to increase positive factors (e.g. perceived relevance) while simultaneously reducing negative factors (e.g. perceived intrusiveness) which both ultimately affect the consumers’ ad responsiveness. We conclude that an ideal location (or receiving context) to trigger mobile ads does not necessarily need to be in the immediate proximity to the offer. We call these locations with a particularly high level of ad responsiveness “honey-spot”. To provide empirical evidence for the importance of the receiving context, we conducted a field experiment among 110 stu-dents for five weeks. Our results support the idea of honey-spots and show a significant impact on the perception of ads. Further, by increasing the spatial distance, we can keep positive fac-tors intact and simultaneously reduce disturbing factors. Our findings contradict the predomi-nant view in previous research that distance is the most important factor for the effectiveness of LBA.
MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo

Significance Mitochondria are intracellular hubs of metabolism that play critical roles in mammals, but knowledge of the metabolic landscape within mitochondria in mammalian tissues remains incomplete. Prior studies have often relied on interrogating metabolites within whole tissue, but this approach does not reveal the behavior of metabolites within specific organelles and cell types. To address this, we generated a transgenic mouse that can express a mitochondrially localized epitope tag (MITO-Tag) with spatiotemporal control to allow for rapid, cell-type-specific immunoisolation of mitochondria from tissues. We demonstrate that these MITO-Tag Mice can be utilized for profiling a variety of molecular components of mitochondria, such as proteins, lipids, and polar metabolites, and thus believe that MITO-Tag Mice will be useful for studying mitochondrial physiology. Mitochondria are metabolic organelles that are essential for mammalian life, but the dynamics of mitochondrial metabolism within mammalian tissues in vivo remains incompletely understood. While whole-tissue metabolite profiling has been useful for studying metabolism in vivo, such an approach lacks resolution at the cellular and subcellular level. In vivo methods for interrogating organellar metabolites in specific cell types within mammalian tissues have been limited. To address this, we built on prior work in which we exploited a mitochondrially localized 3XHA epitope tag (MITO-Tag) for the fast isolation of mitochondria from cultured cells to generate MITO-Tag Mice. Affording spatiotemporal control over MITO-Tag expression, these transgenic animals enable the rapid, cell-type-specific immunoisolation of mitochondria from tissues, which we verified using a combination of proteomic and metabolomic approaches. Using MITO-Tag Mice and targeted and untargeted metabolite profiling, we identified changes during fasted and refed conditions in a diverse array of mitochondrial metabolites in hepatocytes and found metabolites that behaved differently at the mitochondrial versus whole-tissue level. MITO-Tag Mice should have utility for studying mitochondrial physiology, and our strategy should be generally applicable for studying other mammalian organelles in specific cell types in vivo.
Pre-exposure Prophylaxis Awareness and Use Among Cisgender Women at a Sexually Transmitted Disease Clinic

Methods: We report on the demographic and behavioral characteristics of all HIV-negative, cis-women who presented to the Clinic and of those who met Centers for Disease Control and Prevention (CDC) indications for PrEP. In a logistic regression analysis, we evaluated PrEP awareness among cis-women by year and demographic characteristics. We compared PrEP awareness among ciswomen with PrEP awareness among cisgender men who have sex with men and among cisgender men who inject drugs presenting to the Clinic.
Analysis and insights into recombination signals in lumpy skin disease virus recovered in the field

Wide spread incidences of vaccine-like strains of lumpy skin disease virus (LSDV) have recently been reported in a Russian region with a neighboring country that actively vaccinate with a live attenuated LSD vaccine. The use of live-attenuated viruses (LAVs) as vaccines during an active outbreak, creates potential ground for coinfection of hosts and emergence of a strain combining genetic fragments of both parental vaccine and field strains. In this study, we analyse the vaccine-like strain LSDV RUSSIA/Saratov/2017 detected in Saratovskaya oblast, a region sharing border with Kazakhstan. To gain insight into possible recombination signals, a full-genome next-generation sequencing of the viral genome was performed using the Illumina platform. The genome contains the backbone of a live-attenuated vaccine with a patchwork of wild-type field virus DNA fragments located throughout. A total of 27 recombination events were identified. The average distance between the recombination sites was 3400 base pairs (bp). The impact of the recombination events on the virulence and transmission capacity of the identified virus remains to be clarified. These findings provide evidence for the first time of genetic exchanges between closely related strains of capripoxviruses in the field and a vaccine strain, and prompt a revisiting of the vaccination issue for a safe and efficacious prevention and control strategy of LSD.
Equal Access for Everyone? A Digital Divide Cascade for retired Senior Citizens

Society has arrived in the digital era. The continuous transition from an analogue into a digital world provides many benefits for of our daily lives. Yet, digital access is still unevenly distributed and accom- panied by unequal use of digital resources, resulting in social disparities. Especially senior citizens are lagging behind in making use of the digital media environment – despite having the necessary access to IT. To understand the causes and consequences of digital inequalities, we propose and empirically val- idate a digital divide cascade targeted at the retired senior audience in the context of online health information use. We analyze the role of the prior workplace experiences have on exploratory IT behav- ior. The results demonstrate how the digital divide ultimately leads to unequal health knowledge. We thereby contribute to the discussion of seniors’ use of digital resources and derive implications for practice and policy.
Phyllanthin and hypophyllanthin from Phyllanthus amarus ameliorates immune-inflammatory response in ovalbumin-induced asthma: role of IgE, Nrf2, iNOs, TNF-α, and IL’s

Abstract Background: Asthma is a chronic airway immunoinflammatory disorder characterized by airway remodeling. Phyllanthus amarus has been reported to possess antioxidant and anti-inflammatory potential. Aim: To evaluate the possible mechanism of action of isolated phytoconstituents from P. amarus (PA) against ovalbumin (OVA)-induced experimental airway hyperresponsiveness (AHR). Material and method: Phyllanthin and hypophyllanthin were isolated and characterized (HPLC) from the methanolic extract of PA. AHR was induced in Sprague-Dawley rats by OVA-challenged, and animals were treated with PA (50, 100, and 200 mg/kg, p.o.) for 28 days. Results: The HPLC analysis showed the presence of phyllanthin and hypophyllanthin in methanolic extract of PA at RT of 25.243 and 26.832 min, respectively. OVA-induced alterations in hemodynamic parameters, lung functions test, peripheral blood oxygen level, total, and differential cell count in Bronchoalveolar Lavage Fluid was significantly attenuated (p < .05) by PA (100 and 200 mg/kg). It also significantly decreased (p < .05) the levels of total protein and albumin in serum, BALF, and lungs. OVA-induced increase in IgE (total and OVA-specific), and oxido-nitrosative stress (SOD, GSH, MDA, and NO) levels were significantly (p < .05) decreased by PA. RT-PCR analysis revealed that elevated oxido-nitrosative stress (Nrf2 and iNOs), immune-inflammatory makers (HO-1, TNF-α, IL-1β, and TGF-β1), Th2 cytokines (IL-4 and IL-6) levels were significantly attenuated (p < .05) by PA. PA also attenuated histological and ultrastructural aberrations induced by OVA. Conclusion: Results of the present investigation demonstrated that the presence of phyllanthin and hypophyllanthin in P. amarus alleviated Th2 response in OVA-induced AHR via modulation of endogenous markers in a murine model of asthma. Thus, phyllanthin and hypophyllanthin may be a new therapeutic approach for the management of asthma.
Do Verified Consumer Reviews Affect Sales? An Empirical Analysis of Mixed Review Systems in the Film Industry

Consumers refer to reviews in online review systems to collect information for their purchasing decisions. Therefore, the introduction of ‘verified purchase badges’ in some review systems can make an interesting and dynamic impact on product sales. The main objective of this study is to find the differences in the effect of verified and non-verified purchase reviews on product sales from the perspective of WOM metrics (i.e., volume, valence, and helpfulness). Therefore, this study performs an empirical analysis by collecting 866,221 online movie reviews. The results show that volume and valence have a positive effect on the movie sale. However, two group analyses based on whether the ‘verified purchase badge’ or not show some contrasting results. Valence, helpfulness of verified reviews, and volume of non-verified reviews have positive effects on ticket sales. Furthermore, in terms of reviewers’ rating patterns, as the ratio of clusters with a high ratings pattern in the verified group and the ratio of clusters with a varied ratings pattern in the mixed group increase, the effect on sales also increases. Our results break the preconception that all verified purchase reviews always affect sales.
Efficacy of Andrographis paniculata compared to Azadirachta indica, Curcuma longa, and sodium hypochlorite when used as root canal irrigants against Candida albicans and Staphylococcus aureus: An in vitro antimicrobial study

Aim: The aim is to test the antimicrobial activity of Andrographis Paniculata, Azadirachta indica (neem), and Curcuma Longa (curcumin) as a root canal irrigant, against Staphylococcus aureus and Candida albicans using agar diffusion test. Sodium hypochlorite (NaOCl) served as a standard control for comparisons. Materials and Methods: The bacterial strains of C. albicans and S. aureus culture were grown overnight (18–20 h) in the brain heart infusion broth at 37°C and inoculated in Mueller–Hinton agar plates. Antibacterial inhibition was assessed using agar well-diffusion method using the methanolic extracts of the three plants to be tested and NaOCl. Bacterial inhibition zone around each well was recorded. The results were tabulated and analyzed statistically for significance. Results: The novel A. paniculata showed significantly higher zone of inhibition against C. albicans (P < 0.0001) compared to the experimental irrigants. Against S. aureus, it exhibited similar results as that shown by NaOCl (P > 0.05). Conclusion: Zones of inhibition exhibited by novel herbal agent A. paniculata were higher against C. albicans and similar against S. aureus, when compared to NaOCl.
Daytime variation of perioperative myocardial injury in non-cardiac surgery and effect on outcome

Objective Recently, daytime variation in perioperative myocardial injury (PMI) has been observed in patients undergoing cardiac surgery. We aim at investigating whether daytime variation also occurs in patients undergoing non-cardiac surgery. Methods In a prospective diagnostic study, we evaluated the presence of daytime variation in PMI in patients at increased cardiovascular risk undergoing non-cardiac surgery, as well as its possible impact on the incidence of acute myocardial infarction (AMI), and death during 1-year follow-up in a propensity score–matched cohort. PMI was defined as an absolute increase in high-sensitivity cardiac troponin T (hs-cTnT) concentration of ≥14 ng/L from preoperative to postoperative measurements. Results Of 1641 patients, propensity score matching defined 630 with similar baseline characteristics, half undergoing non-cardiac surgery in the morning (starting from 8:00 to 11:00) and half in the afternoon (starting from 14:00 to 17:00). There was no difference in PMI incidence between both groups (morning: 50, 15.8% (95% CI 12.3 to 20.3); afternoon: 52, 16.4% (95% CI 12.7 to 20.9), p=0.94), nor if analysing hs-cTnT release as a quantitative variable (median morning group: 3 ng/L (95% CI 1 to 7 ng/L); median afternoon group: 2 ng/L (95% CI 0 to 7 ng/L; p=0.16). During 1-year follow-up, the incidence of AMI was 1.2% (95% CI 0.4% to 3.2%) among morning surgeries versus 4.1% (95% CI 2.3% to 6.9%) among the afternoon surgeries (corrected HR for afternoon surgery 3.44, bootstrapped 95% CI 1.33 to 10.49, p log-rank=0.03), whereas no difference in mortality emerged (p=0.70). Conclusions Although there is no daytime variation in PMI in patients undergoing non-cardiac surgery, the incidence of AMI during follow-up is increased in afternoon surgeries and requires further study. Clinical trial registration NCT02573532;Results.
Computational and In-Vitro Validation of Natural Molecules as Potential Acetylcholinesterase Inhibitors and Neuroprotective Agents.

BACKGROUND Cholinesterase inhibitors are the first line of therapy for the management of Alzheimer's disease (AD), however, it is now established that they provide only temporary and symptomatic relief, besides, having several inherited side-effects. Therefore, an alternative drug discovery method is used to identify new and safer 'disease-modifying drugs'. METHODS Herein, we screened 646 small molecules of natural origin having reported pharmacological and functional values through in-silico docking studies to predict safer neuromodulatory molecules with potential to modulate acetylcholine metabolism. Further, the potential of the predicted molecules to inhibit acetylcholinesterase (AChE) activity and their ability to protect neurons from degeneration was determined through in-vitro assays. RESULTS Based on in-silico AChE interaction studies, we predicted quercetin, caffeine, ascorbic acid and gallic acid to be potential AChE inhibitors. We confirmed the AChE inhibitory potential of these molecules through in-vitro AChE inhibition assay and compared results with donepezil and begacestat. Herbal molecules significantly inhibited enzyme activity and inhibition for quercetin and caffeine did not show any significant difference from donepezil. Further, the tested molecules did not show any neurotoxicity against primary (E18) hippocampal neurons. We observed that quercetin and caffeine significantly improved neuronal survival and efficiently protected hippocampal neurons from HgCl2 induced neurodegeneration, which other molecules, including donepezil and begacestat, failed to do. CONCLUSION Quercetin and caffeine have the potential as "disease-modifying drugs" and may find application in the management of neurological disorders such as AD.
The Role of Social Media for Collective Behavior Development in response to Natural Disasters

With the emergence of social media, user-generated content from people affected by disasters has gained significant importance. Thus far, research has focused on identifying categories and taxonomies of the types of information being shared among users during times of disasters. However, there is a lack of theorizing with the dynamics of and relationships between the identified concepts. In our current research, we applied probabilistic topic modelling approach to identify topics from Chennai disaster Twitter data. We manually interpreted and further clustered the topics into generic categories and themes, and traced their development over the days of the disaster. Finally, we build a process model to explore an emerging phenomenon on social media during a disaster. We argue that the conditions/activities such as collective awareness, collective concern, collective empathy and support are necessary conditions for people to feel, respond, and act as forms of collective behaviour.
The Use of Victim Video Statements in Family Violence Cases Increases the Rate of Early Guilty Pleas

A natural experiment is used to determine the effect of victim video statements (VVS) on the rate of early guilty pleas in cases of domestic violence. Participants were part of a trial conducted in 2017-2018 by New Zealand Police, in Counties Manukau District, South Auckland. Cases that met the eligibility criteria but did not result in a VVS formed the control group with written statements taken. Analyses were restricted to cases that proceeded to a court hearing (VVS group: n = 168, control group: n = 108). The key question was whether taking a VVS would increase the rate of early guilty pleas. A logistic regression model controlled two additional factors: (a) being bailed or remanded and (b) the seriousness of charges. VVS cases resulted in 44% early guilty pleas compared with 30% for those with written statements. VVS have a statistically significant increase in the odds of making a guilty plea, adjusted odds ratio (AOR) =1.77, 95% confidence interval (CI) = [1.02, 3.06], when controlling for the other factors. The results indicate that VVS can be used to reduce demand pressure on the criminal justice system. The findings are considered in terms of the complex relationship between offenders and victims that commonly results in the retraction of written statements.
Corticosteroid use and increased CXCR2 levels on leukocytes are associated with lumacaftor/ivacaftor discontinuation in cystic fibrosis patients homozygous for the F508del CFTR mutation

Cystic fibrosis (CF) is the most common life-shortening genetic disease and is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Several current therapies aim at improving availability and/or function of the mutant CFTR proteins. The combination therapeutic lumacaftor/ivacaftor (Orkambi, luma/iva) partially corrects folding and potentiates CFTR function impaired by the F508del mutation. Despite the potential for clinical benefit, a substantial number of patients discontinue treatment due to intolerable adverse effects. The aim of the present study is to identify differences between individuals who continued treatment and those who discontinued due to adverse respiratory effects to potentially inform treatment decisions. Clinical data from the year prior to treatment initiation were analyzed from 82 patients homozygous for the F508del mutation treated at the Colorado Adult CF Program. Blood samples were collected from 30 of these subjects before initiation of treatment to examine expression of circulating leukocyte surface antigens and cytokines. Clinical and demographic characteristics were analyzed along with inflammatory markers to determine biomarkers of drug discontinuation. The use of oral prednisone and/or nasal budesonide in the year prior to luma/iva initiation was more prevalent in CF subjects who did not tolerate luma/iva (82% vs. 43%). Increased age, but not gender or initial lung function, was associated with higher probability of discontinuing treatment due to side effects overall. Worse lung function (lower ppFEV1, ppFEF25-75 ≤ 60%) was associated with higher incidence of discontinuing treatment due to pulmonary adverse effects. In a nested cohort of patients, increased surface levels of CXCR2 on CD14+CD16- monocytes were associated with discontinuation. Overall, the patients who tolerated luma/iva were distinguishable from those who did not tolerate the drug based on clinical and cellular markers obtained prior to treatment initiation.
Understanding the Consequences of Technostress: A Non-Linear Perspective

Despite the rise in technostress research, two significant gaps have been overlooked. First, although studies on stress proposed curvilinear relationships, such interactions have rarely been examined in the technostress literature. Second, despite stress being multi-disciplinary and theoretically related to emotions, past technostress studies have rarely adopted transdisciplinary approaches. This paper aims to address these knowledge gaps by adopting the triphasic stress model, the appraisal theory of emotions, and the activation theory to investigate and explain the presence of curvilinear relationships within a mediated and moderated model. Data were collected and analyzed by surveying 215 employees from four different medium-sized US organizations. Our findings suggest that antecedents such as ICT-self-efficacy and presenteeism significantly relate to technostressors through cubic S-shaped interactions, while technostressors exhibit a quadratic U-shaped relation with technoexhaustion, whereas technoexhaustion shows a positive linear relationship with discontinuous usage intention. Furthermore, our results partially support the moderating influence of negative affectivity and mediation effects of technoexhaustion. Through this study, we offer a different theoretical perspective and an innovative understanding of the true nature of the technology and stressors. It also offers insights on designing effective organizational ICT tools.