Miracle-dz/UniFAR-FTUs huggingface dataset:
Radiological Anatomy – Evaluation of Integrative Education in Radiology

Abstract Purpose: Evaluation and analysis of the integrative course “Radiological Anatomy” established since 2007 at the Medical School Hannover (MHH) in comparison with conventional education. Materials and Methods: Anatomy and radiology are usually taught separately with a considerable time lag. Interdisciplinary teaching of these associated subjects seems logical for several reasons. Therefore, the integrative course “Radiological Anatomy” was established in the second year of medical education, combining these two closely related subjects. This interdisciplinary course was retrospectively evaluated by consideration of a student questionnaire and staff observations. The advantages and disadvantages of integrative teaching in medical education are discussed. Results: The course ratings were excellent (median 1; mean 1.3 on a scale of 1 to 6). This is significantly (p < 0.001) better than the average of all evaluated courses in the respective term (grade 2.8). The course improved the anatomical comprehension (90 %) and the students stated that the topics were relevant for their future medical education (90 %). Furthermore, interest in the subject’s anatomy and radiology increased during the course (88 %). According to the students’ suggestions the course was enhanced by a visitation in the Department of Radiology and the additional topic central nervous system. Conclusion: Integrative teaching of anatomy and radiology was well received by the students. Both, anatomical and radiological comprehension and the motivation to learn were improved. However, it should be considered, that the amount of work and time required by the teaching staff is considerably increased compared to traditional teaching. Key Points: Combined teaching of anatomy and radiology may improve anatomical and radiological comprehension. Integrative teaching enhances the students’ motivation to learn and their interest in the subjects. The students’ ratings of our integrative course was better than those of conventional courses. Citation Format: Dettmer S, Schmiedl A, Meyer S et al. Radiological Anatomy – Evaluation of Integrative Education in Radiology. Fortschr Röntgenstr 2013; 185: 838 – 843
Centennial‐to‐millennial hydrologic trends and variability along the North Atlantic Coast, USA, during the Holocene

Geophysical and sedimentary records from five lakes in Massachusetts reveal regionally coherent hydrologic variability during the Holocene. All of the lakes have risen since ~9.0 ka, but multicentury droughts after 5.6 ka repeatedly lowered their water levels. Quantified water level histories from the three best‐studied lakes share >70% of their reconstructed variance. Four prominent low‐water phases at 4.9–4.6, 4.2–3.9, 2.9–2.1, and 1.3–1.2 ka were synchronous across coastal lakes, even after accounting for age uncertainties. The droughts also affected sites up to ~200 km inland, but water level changes at 5.6–4.9 ka appear out of phase between inland and coastal lakes. During the enhanced multicentury variability after ~5.6 ka, droughts coincided with cooling in Greenland and may indicate circulation changes across the North Atlantic region. Overall, the records demonstrate that current water levels are exceptionally high and confirm the sensitivity of water resources in the northeast U.S. to climate change.
Mutagenic and chemotherapeutic activity in L1210 leukemia of several monofunctional alkylating agents.

the cells immunize toward cells of the qualitatively for to mutagenic agents. known intentional exposure of cells to mutagenic agents is a requisite for of variant cells having the properties is streptomycin, and gentamicin are not mutagenic cells. ) in vivo with very relevant in vitro observations. repeated experimental observations over several years on in vitro spon taneously occurring changes of leukemogenic and antigenic properties in L1210 cells are quali tatively similar to our results obtained in vivo with mutagenic agents. It that they found loss in leukemogenicity so frequently and showed antigenic changes as the main cause of it. They conclude that known intentional exposure of cultured cells to mutagenic agents is not a prerequisite for generation of variant cells having loss of tumorigenicity.
Effects of Social Isolation on Neuromuscular Excitability and Aggressive Behaviors in Drosophila: Altered Responses by Hk and gsts1, Two Mutations Implicated in Redox Regulation

Social deprivation is known to trigger a variety of behavioral and physiological modifications in animal species, but the underlying genetic and cellular mechanisms are not fully understood. As we described previously, adult female flies reared in isolation show increased frequency of aggressive behaviors than those reared in a group. Here, we report that isolated rearing also caused significantly altered nerve and muscle excitability and enhanced synaptic transmission at larval neuromuscular junctions (NMJs). We found that mutations of two genes, Hyperkinetic (Hk) and glutathione S-transferase-S1 (gsts1), alter the response to social isolation in Drosophila. Hk and gsts1 mutations increased adult female aggression and larval neuromuscular hyperexcitability, even when reared in a group. Unlike wild type, these behavioral and electrophysiological phenotypes were not further enhanced in these mutants by isolated rearing. Products of these two genes have been implicated in reactive oxygen species (ROS) metabolism. We previously reported in these mutants increased signals from an ROS probe at larval NMJs, and this study revealed distinct effects of isolation rearing on these mutants, compared to the control larvae in ROS-probe signals. Our data further demonstrated modified nerve and muscle excitability by a reducing agent, dithiothreitol. Our results suggest that altered cellular ROS regulation can exert pleiotropic effects on nerve, synapse, and muscle functions and may involve different redox mechanisms in different cell types to modify behavioral expressions. Therefore, ROS regulation may take part in the cellular responses to social isolation stress, underlying an important form of neural and behavioral plasticity.
TNF revisited: TNF‐independent antitumor activity in sera of mice sensitized with Propionibacterium acnes and challenged with lipopolysaccharide

Sera of mice sensitized with bacteria and subsequently challenged with lipopolysaccharide promote hemorrhagic necrosis of tumors in vivo and display cytotoxic activity against tumor cells in vitro, which has been attributed to the induction of tumor necrosis factor (TNF). Here, we describe the induction of a previously unrecognized antitumor activity in such sera, which is distinct from TNF but displays tumor‐specific cytocidal activity in vitro as well as potent tumor‐regressing activity in vivo. Biochemical analysis of this activity yielded a molecular mass of ∼150 kDa, closely resembling a novel tumoricidal factor of murine macrophages (Mφ) termed MTC 170 (Mφ tumor cytotoxin, approximate molecular mass 170 kDa), which we have previously proposed to constitute a major effector pathway for the destruction of tumor cells by activated Mφ.
Estimating time‐varying drug adherence using electronic records: extending the proportion of days covered (PDC) method

Accurate measurement of drug adherence is essential for valid risk‐benefit assessments of pharmacologic interventions. To date, measures of drug adherence have almost exclusively been applied for a fixed‐time interval and without considering changes over time. However, patients with irregular dosing behaviour commonly have a different prognosis than patients with stable dosing behaviour.
Exogenous Learning, Seller-Induced Learning, and Marketing of Durable Goods

When learning of product characteristics takes some time, a firm introducing a new durable faces the trade-off between releasing early to an uninformed market and deferring release to a better-informed market. In a two-period monopoly, we examine the strategic interaction between exogenous learning (EL) and seller-induced learning (SIL) and the firm's product release and pricing strategies. The familiar, direct effect of strong learning is to facilitate a higher price for informed customers. We point out its indirect effect of inducing a higher period 1 price for uninformed customers (by lowering their expected utility from learning). These two effects underlie three major results. First, a strong learning intensity does not always imply deferred release. Surprisingly, for medium unit costs, the firm releases late (early) when learning intensity is weak (strong). Second, SIL facilitates different product release strategies, depending on the unit cost level. Potential SIL investment facilitates early release for low or medium unit costs, but may facilitate deferred release for high unit costs. Lastly, when customers have heterogeneous prior valuation, the high-end customers may buy early at a lower price and the low-end customers may buy later at a higher price, contrary to the usual skim pricing with informed customers. This paper was accepted by Preyas Desai, marketing.
Pharmacokinetic-pharmacodynamic evaluation of ceftazidime continuous infusion vs intermittent bolus injection in septicaemic melioidosis.

AIMS Experimental studies have suggested that constant intravenous infusion would be preferable to conventional intermittent bolus administration of beta-lactam antibiotics for serious Gram-negative infections. Severe melioidosis (Burkholderia pseudomallei infection) carries a mortality over 40% despite treatment with high dose ceftazidime. The aim of this study was to measure the pharmacokinetic and pharmacodynamic effects of continuous infusion of ceftazidime vs intermittent bolus dosing in septicaemic melioidosis. METHODS Patients with suspected septicaemic melioidosis were randomised to receive ceftazidime 40 mg kg(-1) 8 hourly by bolus injection or 4 mg kg(-1) h(-1) by constant infusion following a 12 mg kg(-1) priming dose and pharmacokinetic and pharmacodynamic parameters were compared. RESULTS Of the 34 patients studied 16 (59%) died. Twenty patients had cultures positive for B. pseudomallei of whom 12 (60%) died. The median MIC90 of B. pseudomallei was 2 mg l(-1), giving a minimum target concentration (4*MIC) of 8 mg l(-1). The median (range) estimated total apparent volume of distribution, systemic clearance and terminal elimination half-lives of ceftazidime were 0.468 (0.241-0. 573) l kg(-1), 0.058 (0.005-0.159) l kg(-1) h(-1) and 7.74 (1.95-44.71) h, respectively. Clearance of ceftazidime and creatinine clearance were correlated closely (r = 0.71; P < 0.001) and there was no evidence of significant nonrenal clearance. CONCLUSIONS Simulations based on these data and the ceftazidime sensitivity of the B. pseudomallei isolates indicated that administration by constant infusion would allow significant dose reduction and cost saving. With conventional 8 h intermittent dosing to patients with normal renal function, plasma ceftazidime concentrations could fall below the target concentration but this would be unlikely with a constant infusion. Correction for renal failure, which is common in patients with meliodosis is Clearance = k(*) creatinine clearance where k = 0.72. Calculation of a loading dose gives median (range) values of loading dose, DL of 18.7 mg kg(-1) (9.5-23) and infusion rate I = 3.5 mg k(-1) h(-1) (0.4-13) (which equals 84 mg kg(-1) day(-1)). A nomogram for adjustment in renal failure is given.
BRAF-Mutated Colorectal Cancer Exhibits Distinct Clinicopathological Features from Wild-Type BRAF-Expressing Cancer Independent of the Microsatellite Instability Status

In patients with colorectal cancer (CRC), the BRAF V600E mutation has been reported to be associated with several clinicopathological features and poor survival. However, the prognostic implications of BRAF V600E mutation and the associated clinicopathological characteristics in CRCs remain controversial. Therefore, we reviewed various clinicopathological features, including BRAF status, in 349 primary CRCs and analyzed the relationship between BRAF status and various clinicopathological factors, including overall survival. Similar to previous studies conducted in Eastern countries, the incidence of the BRAF V600E mutation in the current study was relatively low (5.7%). BRAF-mutated CRC exhibits distinct clinicopathological features from wild-type BRAF-expressing cancer independent of the microsatellite instability (MSI) status. This mutation was significantly associated with a proximal tumor location (P = 0.002); mucinous, signet ring cell, and serrated tumor components (P < 0.001, P = 0.003, and P = 0.008, respectively); lymphovascular invasion (P = 0.004); a peritumoral lymphoid reaction (P = 0.009); tumor budding (P = 0.046); and peritoneal seeding (P = 0.012). In conclusion, the incidence of the BRAF V600E mutation was relatively low in this study. BRAF-mutated CRCs exhibited some clinicopathological features which were also frequently observed in MSI-H CRCs, such as a proximal location; mucinous, signet ring cell, and serrated components; and marked peritumoral lymphoid reactions.
CE with LED‐based detection: An update

CE detectors based on LEDs as light sources are attracting considerable attention and having increasing applications. They show excellent stable output with less energy consumption, have long lifetime, and are low‐cost, small in size, and able to work at various wavelengths. This review updates our last review article published in 2007 (Electrophoresis 2007, 28, 233–242) and focuses on the development and application of CE detectors based on LEDs reported in recent years. It describes the exciting new approaches and improved techniques. Three configurations of absorption and fluorescence detection techniques including right‐angle‐type, collinear‐type, and optical‐fiber fluorescence detector are introduced and compared. Lastly, it also discusses their applications and future perspectives in this field.
Pulsed Doppler sonography of the hilar renal artery: differentiation of obstructive from nonobstructive hydronephrosis in children.

BACKGROUND/PURPOSE It is difficult to detect the arcuate or cortical renal arteries when performing pulsed Doppler sonography (PDS) for congenital hydronephrosis. This study was undergone to assess the usefulness of PDS of the hilar renal artery to differentiate obstructive from nonobstructive hydronephrosis. METHODS The authors performed PDS of the hilar renal artery in 80 normal children: 20 aged 0 to 1 months (group I), 20 aged 1 to 12 months (group II), 20 aged 1 to 6 years (group III), and 20 aged 7 to 15 years (group IV). Based on diuretic renography findings, 22 kidneys from 19 children with a ureteropelvic junction (UPJ) stricture were divided into 7 dilated obstructed and 15 dilated nonobstructed kidneys. The peak-systolic velocity (PSV), end-diastolic velocity (EDV), mean average velocity (Vm) and the resistive index (RI = [PSV - EDV]/PSV) were measured at the hilar renal artery. RESULTS There was a significant difference in the RI of the hilar renal artery between obstructive and nonobstructive hydronephrosis. CONCLUSION A pulsed Doppler evaluation of the hilar renal artery is useful for detecting an obstructive UPJ stricture compared with assessing arcuate or cortical renal arteries.
The Proto-Oncogene Bcl3, Induced by Tax, Represses Tax-Mediated Transcription via p300 Displacement from the Human T-Cell Leukemia Virus Type 1 Promoter

ABSTRACT The etiology of human T-cell leukemia virus type 1 (HTLV-1)-induced adult T-cell leukemia is linked to the expression of the viral oncoprotein Tax. Although the mechanism of retroviral transformation is unknown, Tax interferes with fundamental cellular processes, including proliferation and apoptosis, and these events may directly link Tax to early steps in malignant progression. In this study, we examined the interplay between Tax and the potent proto-oncogene B-cell chronic leukemia protein 3 (Bcl3). Bcl3 is a critical regulator of cell survival and proliferation and is overexpressed in HTLV-1-infected cells. We found that Tax induced Bcl3 expression through stimulation of the NF-κB pathway. An intronic NF-κB binding site within the Bcl3 gene served as the primary target of Tax-induced NF-κB activation. We next considered the consequence of Bcl3 overexpression on Tax function. Interestingly, we found that Bcl3 formed a stable complex with Tax and that this complex potently inhibited Tax-dependent HTLV-1 transcription. Importantly, Bcl3 associated with the HTLV-1 promoter in a Tax-dependent manner and inhibited the binding of the critical cellular coactivator p300. The conserved ankyrin repeat domain of Bcl3 mediated both Tax binding and inhibition of p300 recruitment to the HTLV-1 promoter. Together, these data suggest that Tax-induced Bcl3 overexpression benefits the virus in two important ways. First, Bcl3 may promote cell division and thus clonal proliferation of the virus. Second, Bcl3 may attenuate virion production, facilitating immune evasion. One consequence of this regulatory loop may be Bcl3-induced malignant transformation of the host cell.
Outdoor and Indoor Cultivation of Spirulina platensis in the Extreme South of Brazil

Water supplemented with 10% or 20% (v/v) of Zarrouk medium was used to cultivate Spirulina platensis in closed and open bioreactors under controlled conditions (30 °C, 32.5 μmol m−2 s−1, 12 h light/dark photoperiod) and in a greenhouse (9.4 to 46 °C, up to 2800 μmol m−2 s−1, variable day length photoperiod) using different initial biomass concentrations (X0) in the extreme south of Brazil (32.05° S, 52.11° W). Under controlled conditions the maximum specific growth rate (μmax) was 0.102 d−1, the biomass doubling time (td) was 6.8 d, the maximum dry biomass concentration (Xmax) was 1.94 g LD1 and the maximum productivity (Pmax) was 0.059 g L−1 d−1, while the corresponding values in the greenhouse experiments were μmax = 0.322 d−1, td = 2.2 d, Xmax = 1.73 g L−1 and Pmax = 0.112 g L−1 d−1. Under controlled conditions the highest values for these parameters occurred when X0 = 0.15 g L−1, while in the greenhouse X0 = 0.4 g L−1 produced the highest values. These results show that the cultivation of S. platensis in greenhouses in the extreme south of Brazil is technically viable and that the S. platensis inoculum and the concentration of Zarrouk medium can be combined in such a way as to obtain growth and productivity parameters comparable, or superior, to those occurring in bioreactors under controlled conditions of temperature, illuminance and photoperiod.
The effects of inulin, dried Jerusalem artichoke tuber and a multispecies probiotic preparation on microbiota ecology and immune status of the large intestine in young pigs

ABSTRACT The study aimed at determining the effect of two types of prebiotics and a multispecies probiotic on microbiota activity and composition, as well as mucosal immunity in the large intestine of young pigs. In total 48 piglets were divided into 6 groups (n = 8), which received from day 10 of life probiotic-unsupplemented (PU) or probiotic-supplemented (PS) diets. Probiotics were added at 0.5 g/kg diet and contained: Lactococcus lactis, Carnobacterium divergens, Lactobacillus casei, Lactobacillus plantarum and Saccharomyces cerevisiae. The PU and PS diets were formulated without prebiotic addition (control) or with addition of 2% of inulin from chicory root (IN) or 4% of dried Jerusalem artichoke tubers (DJA). After 40 days of feeding, digesta and tissue samples were taken from the caecum and three sections of the colon for analyses of microbiota activity and composition, secretory immunoglobulin A (sIgA) and intraepithelial lymphocytes (IEL). IN diets decreased the caecal digesta pH and β-glucosidase activity but increased propionic, valeric and total short chain fatty acid (SCFA) concentrations compared to control diets. Feeding DJA diets increased caecal valeric acid level, decreased the concentration of isoacids in the colon, reduced β-glucosidase and β-glucuronidase activity in the middle colon and increased Bifidobacterium spp. populations in the proximal and distal colon. PS diets increased the caecal acetic acid and total SCFA level, and Clostridium spp. populations in the distal colon. Neither probiotic nor prebiotics affected sIgA level or IEL number in the large intestine. In conclusion, DJA modified the microbiota ecology in the large intestine of young pigs to a greater extent than IN and the applied probiotic did not enhance effects of prebiotics.
Improving test pattern generation in presence of unknown values beyond restricted symbolic logic

Test generation algorithms considering unknown (X) values are pessimistic if standard n-valued logic algebras are used. This results in an overestimation of the number of signals with X-values and an underestimation of the fault coverage. In contrast, algorithms based on quantified Boolean formula (QBF), are accurate in presence of X-values but have limits with respect to runtime, scalability and robustness. Recently, an algorithm based on restricted symbolic logic (RSL) has been presented which is more accurate than classical three-valued logic and faster than QBF. Nonetheless, this RSL-based approach is still pessimistic and is unable to detect all testable faults. Additionally, it does not allow the accurate identification of untestable faults. In this paper, we improve test pattern generation based on RSL in two directions in order to reduce the accuracy-gap to QBF further. First, we present techniques to go beyond the accuracy of RSL when generating test patterns. Second, we include a check which is able to accurately identify untestable faults. Experimental results show the high efficiency of the proposed method. It is able to classify almost all faults - either by generating a test pattern or proving untestability.
Prevalence and functions of non-suicidal self-injury in Spanish adolescents.

BACKGROUND This study examined the prevalence, characteristics and functions of Non-suicidal Self-injury (NSSI) among Spanish adolescents. METHOD The sample consisted of 1,864 adolescents aged between 12 and 19 years (Mean Age = 15.32, SD = 1.97, 51.45% girls). The participants completed a modified version of the self-report scale Functional Assessment of Self-Mutilation (FASM; Lloyd, Kelley, & Hope, 1997) to assess rates and methods of NSSI used during the last 12 months. They also indicated the functions of NSSI. RESULTS NSSI behaviors are common among Spanish adolescents. More than half of the sample showed such behavior in the past year, and 32.2% had carried out severe NSSI behaviors. The functions of NSSI were examined by using confirmatory factor analyses. Results supported a hierarchical model consisting of two second-order factors: automatic reinforcement, which explained both positive and negative automatic reinforcement, and social reinforcement, which explained both positive and negative social reinforcement. CONCLUSIONS These dimensions are critical to understand the factors that maintain NSSI behavior and have implications for treatments.
Laboratory studies of binge eating disorder.

INTRODUCTION Because of questions about the accuracy of reported food intake, and in order to study psychological and environmental factors which might affect eating, a number of investigators have examined the eating behavior of individuals with binge eating disorder (BED) under controlled conditions that permit food consumption to be measured. METHODS Published studies that evaluated the eating behavior of individuals with BED in a laboratory were reviewed. RESULTS In laboratory meals, individuals with BED consumed more kilocalories than individuals of similar weight who did not meet criteria for BED. These differences are observed consistently when subjects are asked to binge eat and when they are not. CONCLUSIONS In a laboratory setting, individuals with BED exhibit objectively abnormal eating behavior. These data support, but do not prove, the validity of BED as a diagnostic entity.