Miracle-dz/UniFAR-FTUs huggingface dataset:
RAG and HMGB1 create a large bend in the 23RSS in the V(D)J recombination synaptic complexes

During V(D)J recombination, recombination activating gene proteins RAG1 and RAG2 generate DNA double strand breaks within a paired complex (PC) containing two complementary recombination signal sequences (RSSs), the 12RSS and 23RSS, which differ in the length of the spacer separating heptamer and nonamer elements. Despite the central role of the PC in V(D)J recombination, little is understood about its structure. Here, we use fluorescence resonance energy transfer to investigate the architecture of the 23RSS in the PC. Energy transfer was detected in 23RSS substrates in which the donor and acceptor fluorophores flanked the entire RSS, and was optimal under conditions that yield a cleavage-competent PC. The data are most easily explained by a dramatic bend in the 23RSS that reduces the distance between these flanking regions from >160 Å in the linear substrate to <80 Å in the PC. Analysis of multiple fluorescent substrates together with molecular dynamics modeling yielded a model in which the 23RSS adopts a U shape in the PC, with the spacer located centrally within the bend. We propose that this large bend facilitates simultaneous recognition of the heptamer and nonamer, is critical for proper positioning of the active site and contributes to the 12/23 rule.
Vitamin E and lycopene reduce coal burning fluorosis-induced spermatogenic cell apoptosis via oxidative stress-mediated JNK and ERK signaling pathways

Although fluoride has been widely used in toothpaste, mouthwash, and drinking water to prevent dental caries, the excessive intake of fluoride can cause fluorosis which is associated with dental, skeletal, and soft tissue fluorosis. Recent evidences have drawn the attention to its adverse effects on male reproductive system that include spermatogenesis defect, sperm count loss, and sperm maturation impairment. Fluoride induces oxidative stress through the activation of mitogen activated protein kinase (MAPK) cascade which can lead to cell apoptosis. Vitamin E (VE) and lycopene are two common antioxidants, being protective to reactive oxygen species (ROS)-induced toxic effects. However, whether and how these two antioxidants prevent fluoride-induced spermatogenic cell apoptosis are largely unknown. In the present study, a male rat model for coal burning fluorosis was established and the histological lesions and spermatogenic cell apoptosis in rat testes were observed. The decreased expression of clusterin, a heterodimeric glycoprotein reported to regulate spermatogenic cell apoptosis, was detected in fluoride-treated rat testes. Interestingly, the co-administration with VE or lycopene reduced fluorosis-mediated testicular toxicity and rescued clusterin expression. Further, fluoride caused the enhanced Jun N-terminal kinase (JNK, c-Jun) and extracellular signal-regulated protein kinase (ERK) phosphorylation, which was reduced by VE or lycopene. Thus, VE and lycopene prevent coal burning fluorosis-induced spermatogenic cell apoptosis through the suppression of oxidative stress-mediated JNK and ERK signaling pathway, which could be an alternative therapeutic strategy for the treatment of fluorosis.
Clinical evaluation of 121 lithium disilicate all-ceramic crowns up to 9 years.

OBJECTIVE The aim of this prospective clinical study was to evaluate the clinical performance of lithium disilicate allceramic crowns for a period from 12 to 156 months. METHOD AND MATERIALS One hundred and twenty five lithium disilicate all-ceramic crowns were placed in 35 patients between 2001 and 2007. One patient who received four all-ceramic crowns was excluded from the study. The remaining 34 patients received 121 all-ceramic crowns and were recalled. Ninety-eight anterior and 23 posterior crowns were inserted. All crowns were applied on vital teeth except for 11 crowns which were placed on endodontically treated teeth. Follow-up appointments were performed 6 months after insertion, then annually. Replacement of a restoration was defined as failure. Marginal integrity, marginal discoloration, secondary caries, sensitivity, color match, and ceramic surface were evaluated following modified California Dental Association/Ryge criteria. The survival rate of the crowns was determined using Kaplan- Meier statistical analysis and log-rank test (P = .05). RESULTS Ten crowns were fractured. The cumulative survival rate according to Kaplan-Meier was 87.1% after mean 104.6 months (range 12 to 156 months). Location (anterior vs posterior) of all-ceramic crown did not significantly affect the survival rate according to log-rank test (P = .89). Endodontically treated teeth without post-and-core restorations exhibited a higher failure rate after all-ceramic crown application according to log-rank test (P < .001). CONCLUSION In this in vivo study, lithium disilicate all-ceramic crowns exhibited a satisfactory clinical performance with an estimated survival probability of 87.1% over 104.6 months and they can be used clinically in the anterior and posterior region. Restoring endodontically treated teeth with post-and-core restorations should be considered before all-ceramic crown application.
Moving object detection in the H.264/AVC compressed domain

This paper presents a moving object detection algorithm for H.264/AVC video streams that is applied in the compressed domain. The method is able to extract and analyze several syntax elements from any H.264/AVC-compliant bit stream. The number of analyzed syntax elements depends on the mode in which the method operates. The algorithm is able to perform either a spatiotemporal analysis in a single step or a two-step analysis that starts with a spatial analysis of each frame, followed by a temporal analysis of several subsequent frames. Thereby, in each mode either only (sub-)macroblock types and partition modes or, additionally, quantization parameters are analyzed. The evaluation of these syntax elements enables the algorithm to determine a “weight” for each 4×4 block of pixels that indicates the level of motion within this block. A final segmentation after creating these weights segments each frame to foreground and background and hence indicates the positions and sizes of all moving objects. Our experiments show that the algorithm is able to efficiently detect moving objects in the compressed domain and that it is configurable to process a large number of parallel bit streams in real time.
Role of women in economic development.

Women in rural India play a dual role--producers of goods and services as well as their domestic chores and wives and mothers--yet their contribution to economic development has been neglected. This study aimed to assess the socioeconomic conditions of 200 rural women respondents from the villages of Makanpur (100 women) and Varsaitpur (100 women). In terms of general household activities, women were found to contribute 73% of the labor in Makanpur and 70% in Varsaitpur. Women's contributions were greater in the Scheduled Castes and among Muslims. In terms of agricultural activities, women contributed 40% in Makanpur (66% of agricultural labor) and 40% in Varsaitpur (59% of agricultural labor). Taken together, women's contribution to economic activities was 52% in Makanpur--the less prosperous village--and 49% in Varsaitpur--the village more influenced by technology. The problems most often cited by survey respondents included health, malnutrition, repeated childbearing, and education. If women's participation in economic development is to be enhanced, women must receive the following services: training in income generating activities, easy access to low-interest loans, and family planning services to limit childbearing.
On the Relationship Between Circumplexes: Affect and Wiggins' IAS

This article offers a new method to describe the relationship between two circumplexes, illustrated empirically with Wiggins' (1995) Interpersonal Adjective Scales (IAS) and Yik, Russell, and Steiger's (2004) 12-Point Affect Circumplex Scales (12-PACS). Michael Browne's CIRCUM-extension procedure was used to place each circumplex within the other. Simulations showed this procedure can reveal the number of dimensions of overlap between the two and can estimate the magnitude and location of the overlap. The IAS space overlaps the 12-PACS space on one axis, which falls at 35 degrees within the 12-PACS space (pleasant activated versus unpleasant deactivated) and at 71 degrees within the IAS space, which is close to where Extraversion (Factor I of the Five Factor Model) also lies.
A Controlled Study of the Effect of Sodium Valproate on Photosensitive Epilepsy and Its Prognosis

The effect of sodium valproate on photosensitive epilepsy was studied. The range of flash rates of intermittent photic stimulation to which patients were sensitive (photosensitive range) was tested prior to treatment, and the reliability of this measure established by repeated tests on 70 patients. The photosensitive range was measured on 50 patients prior to and during treatment with sodium valproate. In 27 patients photosensitivity was abolished and in a further 12 patients photosensitivity was significantly reduced. A group of 167 patients followed without treatment did not show significant improvement over a 7 year period. Sixteen patients had drug treatment withdrawn, and in 7 months their photosensitive range had returned to its predrug level.
Gysel type unbalanced-to-balanced equal Power Divider

This paper proposes an Unbalanced-to-Balanced (UTB) Gysel type equal Power Divider (PD). Theoretical analysis has been performed to obtain design equations for the PD. UTB equal PD is design at 2 GHz. Based on the design equations, a prototype has been fabricated and measurement has been performed. The proposed PD shows in-phase characteristic. Greater than 20 dB isolation between two balanced ports is achieved. Greater than 20 dB common mode suppression to two balanced ports is achieved. Differential power is equally divided from unbalanced port to balanced ports.
Increased Dietary Inflammatory Index (DII) Is Associated With Increased Risk of Prostate Cancer in Jamaican Men

Prostate cancer is the most common nonskin malignancy, and it accounts for the most cancer deaths among Jamaican males. Diet has been implicated in the etiology of prostate cancer, including through its effects on inflammation. We examined the association between a newly developed dietary inflammatory index (DII) and prostate cancer in a case-control study of 40–80 yr old Jamaican males. A total of 229 incident cases and 250 controls attended the same urology out-patient clinics at 2 major hospitals and private practitioners in the Kingston, Jamaica metropolitan area between March 2005 and July 2007. The DII was computed based on dietary intake assessed using a previously validated food frequency questionnaire (FFQ) that was expanded to assess diet and cancer in this Jamaican population. Multivariable logistic regression was used to estimate odds ratios, with DII as continuous and expressed as quartiles. Logistic regression analysis adjusted for age, total energy intake, education, body mass index, smoking status, physical activity, and family history of prostate cancer. Men in the highest quartile of the DII were at higher risk of prostate cancer [odds ratio (OR) = 2.39; 95% confidence interval (CI) = 1.14–5.04; Ptrend = 0.08] compared to men in the lowest DII quartile. These data suggest a proinflammatory diet, as indicated by increasing DII score, may be a risk factor for prostate cancer in Jamaican men.
Subclinical Abnormal Gyration Pattern, a Potential Anatomic Marker of Epileptogenic Zone in Patients With Magnetic Resonance Imaging–Negative Frontal Lobe Epilepsy

BACKGROUND:Epilepsy surgery for magnetic resonance imaging (MRI)-negative patients has a less favorable outcome. OBJECTIVE:Detection of subclinical abnormal gyration (SAG) patterns and their potential contribution to assessment of the topography of the epileptogenic zone (EZ) is addressed in MRI-negative patients with frontal lobe epilepsy. METHODS:Between September 1998 and July 2005, 12 MRI-negative frontal lobe epilepsy patients underwent stereoelectroencephalography with postcorticectomy follow-up of longer than 1 year (average, 3.3 years). Original software (BrainVISA/Anatomist, http://brainvisa.info) trained on a database of normal volunteers was used to determine which sulci had morphology out of the normal range (SAG). Topography of the EZ, SAG pattern, corticectomy, postoperative seizure control, and histopathology were analyzed. RESULTS:At last follow-up, 8 of 12 patients (66.7%) were Engel class I (7 IA and 1 IB), 2 class II, and 2 class IV. Small focal cortical dysplasia was histologically diagnosed in 9 of the 12 patients (75%), including 7 of 8 seizure-free patients (87.5%). A SAG pattern was found to be in the EZ area in 9 patients (75%), in the ipsilateral frontal lobe out of the EZ in 2, and limited to the contralateral hemisphere in 1. CONCLUSION:SAG patterns appear to be associated with the topography of the EZ in MRI-negative frontal lobe epilepsy and may have a useful role in preoperative assessment. Small focal cortical dysplasia not detected with MRI is often found on histopathological examination, particularly in the depth of the posterior part of the superior frontal sulcus and intermediate frontal sulcus, suggesting a specific developmental critical zone in these locations.
Learning to modulate transmitter release: themes and variations in synaptic plasticity.

Learning is the modification of behavior by experience, and memory is the retention of that modification over time. As learning and memory have become accessible to study with the techniques of cellular and molecular biology, a variety of cellular mechanisms of neuronal plasticity have now been identified that are thought to contribute to different forms of learning in invertebrates and vertebrates (for reviews, see Alkon & Rasmussen 1988; Byrne 1987; Carew & Sahley 1986; Hawkins et al 1987; Ito 1 989; Nicoll et a11988; Thompson 1986). Therefore, researchers can now attempt to define basic principles of learning by comparing these mechanisms and asking: What do they have in common, and how are they different? Until quite recently, these questions proved surprisingly difficult to answer. Different forms of neuronal plasticity appeared to predominate in different forms oflearning, in different regions of the brain, and in different species. In mammals, for example, at least two quite different synaptic mechanisms appeared to operate in different regions of the hippocampus, a structure critical for learning. Moreover, these two mechanisms seemed to differ from those encountered in such invertebrates as Aplysia, Her­ missenda, and Drosophila. However, recent cellular studies on hippo-
Evaluation of Morphological Diversity, Mitotic Instability and Fertility in Tritipyrum

Tritipyrum is prone to mitotic instability, stiff straw and low fertility. A complete randomized design was used to evaluate morphological diversity, fertility and grain yield of 7 new synthetic Tritipyrum lines and their F1 offspring’sin a cross with bread wheat. Fertility, harvest index, biological yield, 1000-grains weight (TGW) and grain yield of Tritipyrum derived genotypes were significantly lower than bread wheat and Triticale. The morphology of Tritipyrum was found to be similar with wheat lines and hybridization between Tritipyrum and bread wheat was efficient for developing early maturelines and reducing stiff straw and plant height. Results showed that mitotic instability in small size grains (TGW 30 g) in both parental and F2progenies . Euploidyof Tritipyrum derived genotypes were significantly lower than Triticale and bread wheat. Results suggested that the larger and heavier grains show higher frequency of euploidy and that can be useful for maintenance of the genetic stability of Tritipyrum genotypes. Tritipyrum as a new man made cereal is a good genetic resource for wheat breeding and may complements the role of bread wheat wherever environmental condition is not suitable for wheat cultivation.
Suppression of renal cell proliferation, induction of apoptosis and cell cycle arrest: Cytotoxicity of vanadium in broilers

The aims of this study were to clarify the effects of high vanadium on the renal cell cycle and apoptosis in broilers. 420 one-day-old avian broilers were divided into six groups and fed on a control diet (vanadium 0.073 mg/kg), and five high vanadium diets (vanadium 5 mg/kg, high vanadium group I; 15 mg/kg, high vanadium group II; 30 mg/kg, high vanadium group III; 45 mg/kg, high vanadium group IV; 60 mg/kg, high vanadium group V) throughout the experimental period of 42 days. As tested by flow cytometry, the percentage of apoptotic renal cells was increased in high vanadium group II, III, IV and V when compared with that of control group. The Proliferating index (PI) of renal cell and the ratio of S, G2 + M phase cells were markedly decreased and population of G0/G1 cells was increased in high vanadium group II, III, IV and V. The results showed that dietary vanadium in excess of 15 mg/kg was toxic to kidney by the renal cells cycle arrest and increased apoptosis, which caused the growth depression of the kidney in broilers.
Expected usability is not a valid indicator of experienced usability

Usability is a core construct of website evaluation and inherently defined as interactive. Yet, when analysing first impressions of websites, expected usability, i.e., before use, is of interest. Here we investigate to what extend ratings of expected usability are related to (a) experienced usability, i.e., ratings after use, and (b) objective usability measures, i.e., task performance. Furthermore, we try to elucidate how ratings of expected usability are correlated to aesthetic judgments. In an experiment, 57 participants submitted expected usability ratings after the presentation of website screenshots in three viewing-time conditions (50, 500, and 10,000 ms) and after an interactive task (experienced usability). Additionally, objective usability measures (task completion and duration) and subjective aesthetics evaluations were recorded for each website. The results at both the group and individual level show that expected usability ratings are not significantly related either to experienced usability or objective usability measures. Instead, they are highly correlated with aesthetics ratings. Taken together, our results highlight the need for interaction in empirical website usability testing, even when exploring very early usability impressions. In our study, user ratings of expected usability were no valid proxy neither for objective usability nor for experienced website usability. Subjects Human–Computer Interaction, World Wide Web and Web Science
Mediastinectomy for management of chronic pyogranulomatous pleural disease in dogs

The medical records of 12 dogs with chronic pyogranulomatous pleural disease unresponsive to medical management were reviewed retrospectively. Resection of the mediastinal pleura (mediastinectomy) was performed through a median sternotomy to remove all diseased and surgically accessible mediastinal pleural tissue. Dogs were re-examined two weeks postoperatively, and long-term outcome was evaluated by contacting owners by phone. Twelve dogs underwent mediastinectomy; additional surgeries included subtotal pericardiectomy (8), lung lobectomy (4) and partial diaphragmatic resection (2). Histology of resected tissue consistently revealed neutrophilic, pyogranulomatous cellulitis/serositis. Foreign material was evident in the mediastinal tissue of five dogs and microorganisms were recovered from three dogs. Two dogs developed pneumothorax immediately postoperatively; one dog developed haemothorax one month postoperatively and was euthanased. Median follow-up time was eight months (range: 6–43 months); eleven dogs were alive and considered to be symptom-free by their owners. Mediastinectomy resulted in complete resolution of symptoms in most dogs (92 per cent) and was associated with a low incidence of major complications. The results of this study indicated that mediastinectomy results in favourable outcome for dogs with chronic pleural pyogranulomatous pleural disease unresponsive to medical management.
Geographical patterns of adaptation within a species’ range: interactions between drift and gene flow

We use individual‐based stochastic simulations and analytical deterministic predictions to investigate the interaction between drift, natural selection and gene flow on the patterns of local adaptation across a fragmented species’ range under clinally varying selection. Migration between populations follows a stepping‐stone pattern and density decreases from the centre to the periphery of the range. Increased migration worsens gene swamping in small marginal populations but mitigates the effect of drift by replenishing genetic variance and helping purge deleterious mutations. Contrary to the deterministic prediction that increased connectivity within the range always inhibits local adaptation, simulations show that low intermediate migration rates improve fitness in marginal populations and attenuate fitness heterogeneity across the range. Such migration rates are optimal in that they maximize the total mean fitness at the scale of the range. Optimal migration rates increase with shallower environmental gradients, smaller marginal populations and higher mutation rates affecting fitness.
Spatial analysis of visceral leishmaniasis in the municipality of Rondonópolis, in the Brazilian State of Mato Grosso, from 2003 to 2012: human, canine and vector distribution in areas of disease transmission.

INTRODUCTION Visceral leishmaniasis (VL) is a zoonosis of great importance to public health and is considered a neglected disease by the World Health Organization. The disease has expanded and become more prevalent in urban areas in Brazil. METHODS Geospatial analyses were performed and thematic maps of the triad of the disease were produced for the study period (2003-2012) in the urban area of the municipality of Rondonópolis in the midwestern State of Mato Grosso (MT), Brazil, TerraView 4.2. 2 software was used for the analyses. RESULTS A total of 87.9% of the 186 confirmed human cases of VL were cured. Children between the ages of 1 and 4 were the most affected. Registered deaths were predominant among adults aged 60 years or older. The urban area of the municipality consists of eight strata and 12 census districts include 237 neighborhoods. All sectors had confirmed cases of VL. During the study period, human cases of the disease were recorded in 90 neighborhoods. The 23 deaths from the disease were distributed in 21 neighborhoods. Sandflies carrying the parasite were captured in 192 out of 200 neighborhoods evaluated for the presence of the VL vector. The presence of dogs carrying the parasite was confirmed in, 140 out of 154 surveyed neighborhoods. CONCLUSIONS The data demonstrated the endemic nature of VL, with a high percentage of infected children, a high distribution of canine infection, and a wide adaptation and dispersal of the vectors in the urban environment. These results, illustrate the process of urbanization of VL in the municipality of Rondonópolis, MT, Brazil.