Miracle-dz/UniFAR-FTUs huggingface dataset:
The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition.

Thermosensitive mutations in the DBF2 gene arrest the cell cycle during nuclear division. Although the chromatin has divided in arrested cells, an elongated mitotic spindle is present and Cdc28 protein kinase activity remains high, indicating that nuclear division is incomplete. By execution point analysis we show that Dbf2 carries out an essential cell cycle function after the metaphase to anaphase transition and is therefore required during anaphase and/or telophase. This cell cycle stage‐specific requirement for the function of Dbf2 coincides with the cell cycle regulation of Dbf2/Dbf20 protein kinase activity, which can be detected in immunoprecipitates containing Dbf2 or Dbf20. The kinase activity is specific for serine/threonine residues and Dbf2 accounts for the bulk of the activity, with Dbf20 playing a minor role. Furthermore, Dbf2 is a phosphoprotein and, significantly, the dephosphorylated form appears with the same cell cycle timing as the kinase activity, suggesting a role for dephosphorylation in the activation mechanism. In addition, we show that the DBF2 transcript, which is under cell cycle control, is expressed in advance of the activation of the kinase, but that cell cycle‐regulated expression of the mRNA is not required for activation of the Dbf2 kinase during M phase. Thus, Dbf2/Dbf20 kinase activity is precisely regulated in the cell cycle by a post‐translational mechanism and phosphorylates its target substrates for an event that occurs during anaphase and/or telophase.
The C677T MTHFR gene mutation is not predictive of risk for recurrent fetal loss

We have investigated the potency of the C677T mutation in the methylene tetrahydrofolate reductase (MTHFR) gene as a genetic risk factor in women with a history of early (12 weeks gestation) and/or late (>12 weeks gestation) recurrent miscarriage (three or more consecutive pregnancy losses). Fifty‐seven of the total 173 (32.9%) patients were heterozygous for the MTHFR mutation, 14/173 (8.1%) were homozygous (allele frequency 0.25). The prevalence of the MTHFR mutation in these women did not differ significantly from that in the control group of parous women with uneventful pregnancies, where 30/67 (44.8%) were heterozygous and 6/67 (9.0%) homozygous for the mutation (allele frequency 0.31; odds ratio for homozygous T/T 0.90, 95% CI 0.30–2.4). There was no association between the trimester of pregnancy loss and MTHFR genotype. We conclude that the C677T MTHFR mutation is not a risk predictor in women with a history of early or late recurrent miscarriage.
Postoperative mediastinitis in open heart surgery patients. Treatment with unilateral or bilateral pectoralis major muscle flap?

AIM the treatment of mediastinitis, after median sternotomy, in open heart surgery operated patients, remains an important and challenging problem in cardiac surgery. The management of this severe complication by means of surgical debridement of the sternum followed by mediastinal closure with pectoralis major muscle flaps, represents one of the best effective and most common modalities of treatment. The aim of the present publication is to demonstrate the good and comparable result in the mediastinal closure by using a single pectoralis muscle flap instead of two pectoralis muscle flaps. METHODS in our hospital, between July 1998 and December 2004, 32 patients with mediastinitis were treated after adult open heart surgery with sternal debridement and pectoralis major muscle flaps. In 19 cases the sternal closure was performed with a single pectoralis muscle (group U) and in 13 cases with both pectoralis muscles (Group B). RESULTS comparing the group U and group B, we did not found statistical differences in the variables of age, sex, associated diseases, previous myocardial infarction, kind of cardiac surgery undertaken, quality of the sternum, type of germen, number of previous sternal debridement performed and time of hospitalization. Patients in group U showed a statistically significant (P=0.001) shorted plastic reconstructive surgery time, an earlier extubation time (non statistically significant) and less need of blood transfusion (non statistically significant). The morbidity and hospital mortality in group U and B did not show any statistical differences. There were three cases of flap related complications in group B and two in group U. One death was recorded in group U (7.69%) and another in group B (5.26%). CONCLUSION mediastinal infection after cardiac surgery can be effectively managed by surgical debridement followed by plastic coverage with a single pectoralis muscle flap. In our experience, this unilateral pectoralis muscle flap technique showed similar results to the classic closure with both pectoralis muscle flaps. The unilateral technique represents a relative low aggressive operation and preserves intact the contralateral pectoralis muscle. Comparing the single pectoralis muscle flap technique with the bilateral pectoralis musle technique, the former is faster, the extubation is earlier and there is less need of postoperative blood transfusions.
Physicians’ perceptions, expectations, and experience with pharmacists at Hamad Medical Corporation in Qatar

Objectives: The purpose of this study was to investigate the physicians’ perceptions, and expectations of their experiences with the pharmacists at Hamad Medical Corporation (HMC) in Qatar. Method: A cross-sectional study was conducted at HMC between January and March 2006 using a validated questionnaire. The self-administered questionnaire was distributed to 500 physicians who were working at HMC comprising Hamad General Hospital, Women’s Hospital, Rumaila Hospital, Al-Amal Hospital, Al Khor Hospital, and primary health centers. The questionnaire was composed of four parts, investigating the physicians’ expectations, experiences, and perceptions of the pharmacists. Results: A total of 205 questionnaires were completed (response rate 41%). A total of 183 physicians (89%) expected the pharmacist to educate patients about safe and appropriate use of drugs, whereas 118 (57%) expected the pharmacist to be available for health-care team consultation during bedside rounds. The indices of physicians showing how comfortable they were with pharmacists, and their expectations of pharmacists, were 61% and 65%, respectively, whereas the index on experience of physicians with pharmacists was lower (15%). Conclusions: Physicians were comfortable with pharmacists and had high expectations of pharmacists in performing their duties. However, physicians reported a poor experience with pharmacists, who infrequently informed them about the effectiveness of alternative drugs, patients experiencing problems with prescribed medications, and who took personal responsibility to resolve any drug-related problem.
Cerebrospinal fluid leak after microsurgical surgery in vestibular schwannomas via retrosigmoidal craniotomy

Abstract Objective: Cerebrospinal fluid (CSF) leak is still a common complication in surgery of vestibular schwannoma, increasing morbidity and prolonging hospital stay. Our single center study was performed to determine the incidences of CSF leaks after microsurgical removal of vestibular schwannoma via a retrosigmoidal approach with two different surgical closure techniques. Methods: Between January 2003 and December 2009 in 81 patients, microsurgical tumor resection using a suboccipital, retrosigmoidal approach was performed with an interdisciplinary ENT and neurosurgical management was performed. In 41 cases, the dural closure was done using a sandwich technique: subdural closure with TissuFleece® respectively Spongostan®, and after that dural suture and epidural Tachosil® were fixed on. In 40 cases, the dura was sealed epidurally with Tachosil after suture. In 65 cases, the posterior wall of the petrous bone was drilled. The closure was performed using muscle and FibrinGlue®. All patients had a minimal follow-up of 1 year. Results: Seven patients (8·6%) developed a CSF fistula. Three patients (3·7%) underwent surgical procedure because of persisting CSF fistula while in four cases (4·9%) spontaneous closure under lumbar drain was observed. Comparing the different techniques of dural sealing, we found in 41 patients with sandwich technique three CSF leaks (7·3%) while there were four CSF leaks (10%) in 40 patients with a single epidurally sealed dural closure (P=0·69). No rhinorrhea or otorhinorrhea was observed. No intracranial infection or meningitis in case of CSF leak occurred. Conclusion: Suture and occlusion of the dura is an important step to prevent CSF leak and postoperative infection. By comparing sandwich technique and single-layer dural sealing, no significant difference could be shown.
Instructive role of aPKCζ subcellular localization in the assembly of adherens junctions in neural progenitors

In the neurogenic phase of CNS development, the proliferating progenitors are found medially within the neuroepithelium. The adherens junctions on the apical membrane of proliferating neural progenitors allow for cell–cell adhesion and medial stratification. In contrast, differentiating neuronal precursors delaminate and migrate laterally, establishing the laminar layers. Apical adherens junctions also establish the apical–basal polarity in neural progenitors, which in turn is postulated to lead to asymmetric inheritance of cell fate determinants during neurogenic divisions. The signaling pathways and cellular mechanisms that regulate the assembly and asymmetric localization of adherens junctions in neural progenitors remain elusive. Here we show that atypical PKCζ/λ (aPKCζ/λ) localizes at the apical membrane of proliferating neural stem cells, but not postmitotic neuronal precursors, in the developing chicken neural tube. This precise subcellular compartmentalization of the kinase activity provides an instructive signal for apical assembly of adherens junctions in a PI3K, Rac/Cdc42 signaling-dependent pathway. Apical aPKCζ coordinates neural stem cell proliferation and the overall stratification of cell types within the neural tube.
A time–motion analysis of professional rugby league match-play

Abstract The aim of this study was to analyse movement patterns of professional rugby league players during matches played as part of Australia's National Rugby League (NRL) competition. The movement patterns of one player from each of the three positional groups (hit-up forwards, adjustables, and outside backs) during three competition matches were examined using time–motion analysis. Positional groups differed in distances covered (P = 0.001), and covered shorter distances than those described in earlier research. The mean exercise-to-rest ratio was 1:6 for the outside backs and hit-up forwards and 1:5 for the adjustables. However, such ratios did not reflect the most demanding periods of the game, which included repeated high-intensity efforts interspersed with recovery periods of short duration. These periods of repeated high-intensity exercise often occurred at crucial phases of the game, when players were either attacking or defending the try-line. Furthermore, patterns of movement during repeated high-intensity periods of play differed among positional groups. To prepare for the most highly intense periods of match-play, professional rugby league players should adopt position-specific training that includes the highest and lowest exercise-to-rest ratios likely to be experienced in competition.
Endonuclease IV (nfo) mutant of Escherichia coli

A cloned gene, designated nfo, caused overproduction of an EDTA-resistant endonuclease specific for apurinic-apyrimidinic sites in DNA. The sedimentation coefficient of the enzyme was similar to that of endonuclease IV. An insertion mutation was constructed in vitro and transferred from a plasmid to the Escherichia coli chromosome. nfo mutants had an increased sensitivity to the alkylating agents methyl methanesulfonate and mitomycin C and to the oxidants tert-butyl hydroperoxide and bleomycin. The nfo mutation enhanced the killing of xth (exonuclease III) mutants by methyl methanesulfonate, H2O2, tert-butyl hydroperoxide, and gamma rays, and it enhanced their mutability by methyl methanesulfonate. It also increased the temperature sensitivity of an xth dut (dUTPase) mutant that is defective in the repair of uracil-containing DNA. These results are consistent with earlier findings that endonuclease IV and exonuclease III both cleave DNA 5' to an apurinic-apyrimidinic site and that exonuclease III is more active. However, nfo mutants were more sensitive to tert-butyl hydroperoxide and to bleomycin than were xth mutants, suggesting that endonuclease IV might recognize some lesions that exonuclease III does not. The mutants displayed no marked increase in sensitivity to 254-nm UV radiation, and the addition of an nth (endonuclease III) mutation to nfo or nfo xth mutants did not significantly increase their sensitivity to any of the agents tested.
Assessment of prior image induced nonlocal means regularization for low‐dose CT reconstruction: Change in anatomy

Purpose Repeated computed tomography (CT) scans are prescribed for some clinical applications such as lung nodule surveillance. Several studies have demonstrated that incorporating a high‐quality prior image into the reconstruction of subsequent low‐dose CT (LDCT) acquisitions can either improve image quality or reduce data fidelity requirements. Our proposed previous normal‐dose image induced nonlocal means (ndiNLM) regularization method for LDCT is an example of such a method. However, one major concern with prior image based methods is that they might produce false information when the prior image and the current LDCT image show different structures (for example, if a lung nodule emerges, grows, shrinks, or disappears over time). This study aims to assess the performance of the ndiNLM regularization method in situations with change in anatomy. Method We incorporated the ndiNLM regularization into the statistical image reconstruction (SIR) framework for reconstruction of subsequent LDCT images. Because of its patch‐based search mechanism, a rough registration between the prior image and the current LDCT image is adequate for the SIR‐ndiNLM method. We assessed the performance of the SIR‐ndiNLM method in lung nodule surveillance for two different scenarios: (a) the nodule was not found in a baseline exam but appears in a follow‐up LDCT scan; (b) the nodule was present in a baseline exam but disappears in a follow‐up LDCT scan. We further investigated the effect of nodule size on the performance of the SIR‐ndiNLM method. Results We found that a relatively large search‐window (e.g., 33 × 33) should be used for the SIR‐ndiNLM method to account for misalignment between the prior image and the current LDCT image, and to ensure that enough similar patches can be found in the prior image. With proper selection of other parameters, experimental results with two patient datasets demonstrated that the SIR‐ndiNLM method did not miss true nodules nor introduce false nodules in the lung nodule surveillance scenarios described above. We also found that the SIR‐ndiNLM reconstruction shows improved image quality when the prior image is similar to the current LDCT image in anatomy. These gains in image quality might appear small upon visual inspection, but they can be detected using quantitative measures. Finally, the SIR‐ndiNLM method also performed well in ultra‐low‐dose conditions and with different nodule sizes. Conclusions This study assessed the performance of the SIR‐ndiNLM method in situations in which the prior image and the current LDCT image show substantial anatomical differences, specifically, changes in lung nodules. The experimental results demonstrate that the SIR‐ndiNLM method does not introduce false lung nodules nor miss true nodules, which relieves the concern that this method might produce false information. However, there is insufficient evidence that these findings will hold true for all kinds of anatomical changes.
Evaluation of methods for estimation of total arterial compliance.

Seven classic and recently proposed methods used for the estimation of total arterial compliance have been evaluated for their accuracy and applicability in different physiological conditions. The pressure and flow data are taken from a computer model that provides realistic simulations of the nonlinear-distributed systemic arterial tree. Besides the great flexibility in simulating different physiological or pathological cases, the major advantage of the computer model is that it allows precise knowledge of the pressure-dependent total arterial compliance, which is the variable of interest. The results show that the methods based on the two-element windkessel (WK) model are more accurate than those based on the three-element WK model. The classic exponential decay and the diastolic area method yield essentially similar results, and their compliance estimates are accurate within 10% except at high heart rates. The later part of diastole, i.e., from the time that the systolic pressure wave has reached all peripheral beds, gives the best results. The newly proposed two-area and pulse pressure methods, both based on the two-element WK model, are accurate (errors in general < 10%) and can be applied to other locations in the arterial tree where the decay time and area method cannot. Methods based on the three-element WK model consistently overestimate total arterial compliance (> or = 25%). The errors in the methods based on the three-element WK model arise from the fact that the input impedance in that model deviates significantly from the true input impedance at low frequencies. The strong dependence of compliance on pressure (elastic nonlinearity) does not invalidate the compliance estimates. (ABSTRACT TRUNCATED AT 250 WORDS)
Determinants of severe hypoglycemia complicating type 2 diabetes: the Fremantle diabetes study.

CONTEXT There are limited published data characterizing severe hypoglycemia complicating type 2 diabetes. OBJECTIVE The objective of the study was to determine the incidence and predictors of severe hypoglycemia in community-dwelling type 2 patients. DESIGN This was a longitudinal observational cohort study. SETTING This was a community-based study. PATIENTS There were 616 patients (mean age 67.0 yr, 52.3% males, median diabetes duration 7.7 yr) assessed in 1998 and followed up to the end of June 2006. MAIN OUTCOME MEASURES Severe hypoglycemia defined as that requiring ambulance attendance, emergency department services, and/or hospitalization. Cox proportional hazards modeling was used to determine predictors of first episode, and Poisson, negative binomial, zero-inflated Poisson, and zero-inflated negative binomial regression models identified predictors of frequency. RESULTS Fifty-two (8.4%) experienced 66 episodes during 3953 patient-years (incidence 1.7 per 100 patient-years). Those experiencing severe hypoglycemia had one to four episodes. Significant independent predictors of time to first episode were duration of insulin treatment, estimated glomerular filtration rate less than 60 ml/min per 1.73 m(2), peripheral neuropathy, education beyond primary level, and past severe hypoglycemia. The zero-inflated negative binomial provided the best model of severe hypoglycemia frequency. Lower fasting serum glucose and higher glycosylated hemoglobin were significantly associated with frequency, whereas patients at minimal risk of repeated severe hypoglycemia were unlikely to use insulin or to have short-duration insulin treatment, to have renal impairment or peripheral neuropathy, or to be educated beyond primary level. CONCLUSIONS Duration of insulin treatment was confirmed as an independent risk factor for severe hypoglycemia. The novel association with educational attainment suggests knowledge-driven intensive glycemic self-management. The positive relationship between frequency and glycosylated hemoglobin may identify patients with unstable glycemic control.
EVALUATION OF FERMENTED PALM KERNEL MEAL AND FERMENTED COPRA MEAL PROTEINS AS SUBSTITUTE FOR SOYBEAN MEAL PROTEIN IN LAYING HENS DIETS

Two hundred and ten (210) laying hens of Black Harco breed at 37 weeks in lay were fed experimental layer diets, in which fermented palm kernel meal (PKM) and copra meal (CM) were used independently to substitute for soybean meal (SBM) on protein content basis at 0%, 25%, 50% and 75%, to give seven treatments in a completely randomized design feeding trial that lasted 12 weeks. Performance characteristics and some haematological indices were evaluated in this study. Fermentation for seven days increased the crude protein of PKM (from 20.04% to 23.42%) and that of CM (from 19.63% to 23.11%). The crude fibre of the fermented PKM and CM decreased (from 15.47% to 12.44 % and 16.00% to 11.63% respectively). The feed intake (FI) was significantly highest (P 0.05) by the substitution of PKM and CM for SBM. The study showed that SBM protein could be substituted optimally at 50% by either PKM or CM protein.
Time and harmonic study of strongly controllable group systems, group shifts, and group codes

In this paper we give a complementary view of some of the results on group systems by Forney and Trott. We find an encoder of a group system which has the form of a time convolution. We consider this to be a time domain encoder while the encoder of Forney and Trott is a spectral domain encoder. We study the outputs of time and spectral domain encoders when the inputs are the same, and also study outputs when the same input is used but time runs forward and backward. In an abelian group system, all four cases give the same output for the same input, but this may not be true for a nonabelian system. Moreover, time symmetry and harmonic symmetry are broken for the same reason. We use a canonic form, a set of tensors, to show how the outputs are related. These results show there is a time and harmonic theory of group systems.