Miracle-dz/UniFAR-FTUs huggingface dataset:
The Rise Of Income Inequality In Oecd Countries

The objective of this paper is to identify the determinants of the increase in income inequality that rich countries have experienced over the last two decades. My hypothesis is that along with the financialisation of economies that has taken place since 1990, inequality increased because labour flexibility intensified, labour market institutions weakened as trade unions lost power, and public social spending started to retrench and did not compensate the vulnerabilities created by the globalization process. Using data from 34 OECD countries from 1990 to 2013, I empirically evaluate this hypothesis. My results clearly suggest that the increase in inequality over the last two decades is caused by an increase in financialisation, a deepening of labour flexibility, the weakening of trade unions, and the retrenchment of the welfare state.
English Vowels and Consonants Mispronunciation of the Seventh President of Republic of Indonesia in His Official English Speeches and Its Influencing Factors

This research aimed to find out the mispronunciation of English vowels and consonants of the seventh president of Republic of Indonesia, Mr. Joko Widodo in his official English speeches based on the standard of British English Received Pronunciation (RP) and the factors influencing his English vowels and consonants mispronunciation. This research is a descriptive qualitative research. In collecting data, the researchers used observation method with non-participatory technique (Sudaryanto, 2015). In analyzing the data, the researchers used articulatory identity method (Sudaryanto, 2015). It was found that the consonant sounds /θ/, /ð/, /v/, /z/, /ʃ/ were pronounced incorrectly, the vowel sounds/ə/, /ɒ/, /ɛ/, /i/, /e/, /ɪ/ were pronounced inconsistently, and the diphthong sounds /ɪə/, /eɪ/, /əʊ/ and /aʊ/ were pronounced incorrectly. The consonant sound /l/ in the middle of the word was not pronounced. The consonant sound /j/ in the middle of the word is omitted. The consonant sounds /g/, /tʃ/, and /r/ were pronounced the same as the spelling. The consonant sounds /t/, /s/, /k/ at the end of the words were omitted. The letter y sounded /ɪ/ at the end of the word was pronounced as /e/. The diphthong sounds /ɪə/, /eɪ/, /əʊ/ and /aʊ/ were pronounced as /ɪ/, /ʌ/, /ɛ/, /ə/, /e/, and /ͻ/. The factors influencing the mispronunciation of English vowel and consonant sounds were mother tongue interference, sound system differences between Indonesian and English, the influence of spelling on pronunciation, educational background, and environmental background.
Cooking Fuel Choice in Urban Zambia: Implications on Forest Cover

This study examined the use of various sources of cooking energy among urban households in Zambia, and analyzed urban households’ energy choice and charcoal consumption decisions using econometric models. Overall, charcoal is the most common source of main cooking energy in urban areas, followed by electricity, and lastly the other non-specific sources, such as gas, and kerosene. Of the three main sources considered in this study (i.e., electricity only, charcoal only, and a mix of charcoal and electricity) charcoal accounted for almost half (44%) of urban households, followed by a mix of charcoal and electricity representing 38%, and lastly electricity only accounting for 17% of urban households.
Both the Hydrophobicity and a Positively Charged Region Flanking the C-Terminal Region of the Transmembrane Domain of Signal-Anchored Proteins Play Critical Roles in Determining Their Targeting Specificity to the Endoplasmic Reticulum or Endosymbiotic Organelles in Arabidopsis Cells[W]

This study uses in vivo targeting, subcellular fractionation, and bioinformatics approaches to investigate the signal sequences that determine whether signal-anchored proteins are targeted to the endoplasmic reticulum or to endosymbiotic organelles. Proteins localized to various cellular and subcellular membranes play pivotal roles in numerous cellular activities. Accordingly, in eukaryotic cells, the biogenesis of organellar proteins is an essential process requiring their correct localization among various cellular and subcellular membranes. Localization of these proteins is determined by either cotranslational or posttranslational mechanisms, depending on the final destination. However, it is not fully understood how the targeting specificity of membrane proteins is determined in plant cells. Here, we investigate the mechanism by which signal-anchored (SA) proteins are differentially targeted to the endoplasmic reticulum (ER) or endosymbiotic organelles using in vivo targeting, subcellular fractionation, and bioinformatics approaches. For targeting SA proteins to endosymbiotic organelles, the C-terminal positively charged region (CPR) flanking the transmembrane domain (TMD) is necessary but not sufficient. The hydrophobicity of the TMD in CPR-containing proteins also plays a critical role in determining targeting specificity; TMDs with a hydrophobicity value >0.4 on the Wimley and White scale are targeted primarily to the ER, whereas TMDs with lower values are targeted to endosymbiotic organelles. Based on these data, we propose that the CPR and the hydrophobicity of the TMD play a critical role in determining the targeting specificity between the ER and endosymbiotic organelles.
SUMO E3 Ligase SlSIZ1 Facilitates Heat Tolerance in Tomato

High temperature has become a major abiotic stress that limits crop productivity. Heat shock transcription factors (HSFs) and heat shock proteins (HSPs) play important roles in enhancing thermotolerance of plants. SUMOylation is an important post-translational modification in regulating cellular functions in eukaryotes. SIZ1, a well-characterized SUMO E3 ligase, mediates the process of SUMOylation. In this study, SUMO conjugations were clearly induced by high temperature. Overexpression of SIZ1 SUMO E3 ligase (SlSIZ1) in tomato could enhance the tolerance to heat stress in tomato. The RNA interference (RNAi) plants were more wilted than the wild type with heat treatment. Under heat stress, SlSIZ1 could decrease the accumulation of reactive oxygen species (ROS) and induce some genes of HSF and HSP transcription. Furthermore, overexpression of SlSIZ1 could increase the level of Hsp70 under high temperature. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays showed that SlSIZ1 could interact with SlHsfA1 to mediate the SUMOylation of SlHsfA1 and consequently enhance thermotolerance of tomato. In conclusion, overexpression of SlSIZ1 enhanced heat tolerance by regulating the activities of HsfA1 and increasing the content Hsp70.
Preliminary Molecular Characterization of Cryptosporidium parvum Isolates of Wildlife Rodents From Poland

Isolates of Cryptosporidium were collected from 3 species of woodland and field rodents (Clethrionomys glareolus, Microtus arvalis, and Apodemus flavicollis) and were characterized by polymerase chain reaction amplification and sequencing of fragments of the oocyst wall protein (COWP) gene and of the 18S ribosomal RNA gene. Sequence analysis of these markers revealed that the animals were infected with C. parvum, and that the genotype involved was almost identical to the mouse genotype previously described from Mus musculus. Thus, small rodents should be considered as an important reservoir of C. parvum genotypes closely related to the zoonotic genotype 2 and potentially hazardous to humans.
Predictors of Outcome in Term Infants With Neonatal Seizures Subsequent to Intrapartum Asphyxia

The objective of this study was to define potential clinical prognostic factors for term infants with neonatal seizures subsequent to intrapartum asphyxia. The authors completed a retrospective analysis of 62 term infants with clinical neonatal seizures subsequent to intrapartum asphyxia. Logistic regression analysis was applied to determine the independent prognostic indicators of an adverse outcome. A total of 23 (37%) infants had a normal outcome, 34 (55%) survived with 1 or more neurodevelopmental impairments (23 cerebral palsy, 28 global developmental delay, 15 epilepsy, with 18 combination of two, and 9 all three), and 5 (8%) died. Six variables were associated with an adverse outcome, but only the presence of meconium aspiration, a low (≤ 3) 1-minute Apgar score, seizure type other than focal clonic, and moderately severely abnormal electroencephalography (EEG) background findings were independently associated with an adverse outcome. Signs of acute distress are predictors of adverse outcome, alongside seizure semiology and moderate to severe EEG background abnormalities.
Cell‐penetrating and endoplasmic reticulum‐locating TAT‐IL‐24‐KDEL fusion protein induces tumor apoptosis

Interleukin‐24 (IL‐24) is a unique IL‐10 family cytokine that could selectively induce apoptosis in cancer cells without harming normal cells. Previous research demonstrated that intracellular IL‐24 protein induces an endoplasmic reticulum (ER) stress response only in cancer cells, culminating in apoptosis. In this study, we developed a novel recombinant fusion protein to penetrate into cancer cells and locate on ER. It is composed of three distinct functional domains, IL‐24, and the targeting domain of transactivator of transcription (TAT) and an ER retention four‐peptide sequence KDEL (Lys‐Asp‐Glu‐Leu) that link at its NH2 and COOH terminal, respectively. The in vitro results indicated that TAT‐IL‐24‐KDEL inhibited growth in bladder cancer cells, as well as in non‐small cell lung cancer cell line and breast cancer cell line, but the normal human lung fibroblast cell line was not affected, indicating the cancer specificity of TAT‐IL‐24‐KDEL. Western blot analysis showed that apoptosis activation was induced by TAT‐IL‐24‐KDEL through the ER stress‐mediated cell death pathway. Treatment with TAT‐IL‐24‐KDEL significantly inhibited the growth of human H460 xenografts in nude mice, and the tumor growth inhibition was correlated with increased hematoxylin and eosin (H&E) staining and terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) staining. These findings suggest that the artificially designed recombinant fusion protein TAT‐IL‐24‐KDEL may be highly effective in cancer therapy and worthy of further evaluation and development. J. Cell. Physiol. 230: 84–93, 2016. © 2015 Wiley Periodicals, Inc.
Natural Killer Cells in Antifungal Immunity

Invasive fungal infections are still an important cause of morbidity and mortality in immunocompromised patients such as patients suffering from hematological malignancies or patients undergoing hematopoietic stem cell transplantion. In addition, other populations such as human immunodeficiency virus-patients are at higher risk for invasive fungal infection. Despite the availability of new antifungal compounds and better supportive care measures, the fatality rate of invasive fungal infection remained unacceptably high. It is therefore of major interest to improve our understanding of the host–pathogen interaction to develop new therapeutic approaches such as adoptive immunotherapy. As experimental methodologies have improved and we now better understand the complex network of the immune system, the insight in the interaction of the host with the fungus has significantly increased. It has become clear that host resistance to fungal infections is not only associated with strong innate immunity but that adaptive immunity (e.g., T cells) also plays an important role. The antifungal activity of natural killer (NK) cells has been underestimated for a long time. In vitro studies demonstrated that NK cells from murine and human origin are able to attack fungi of different genera and species. NK cells exhibit not only a direct antifungal activity via cytotoxic molecules but also an indirect antifungal activity via cytokines. However, it has been show that fungi exert immunosuppressive effects on NK cells. Whereas clinical data are scarce, animal models have clearly demonstrated that NK cells play an important role in the host response against invasive fungal infections. In this review, we summarize clinical data as well as results from in vitro and animal studies on the impact of NK cells on fungal pathogens.
Diets of denning female Pacific martens vary with the developmental stage of their kits

Abstract Food resources can be a limiting factor and natural and anthropogenic influences that alter the abundance of food resources can affect population performance and persistence. Reproduction in mammals is energetically costly; therefore, understanding how food resources influence reproduction is essential, especially for species of conservation concern. The objectives of this study were to characterize Pacific marten (Martes caurina) diets during the denning period and determine whether diets differed by sex or by phase of the denning period. We used 943 scats to reconstruct sex‐specific diets of martens in the Sierra Nevada mountains during the denning period to evaluate sex‐specific hypotheses of predation patterns. During the lactation phase, females preyed primarily on large‐sized prey (62.5% metabolizeable energy) 5.7‐times more than males. This likely optimized both energy gain and minimized time spent away from dependent young. During the weaning phase, females preyed primarily on medium‐ (90–200 g) and large‐sized prey (87.7% metabolizeable energy). During the predispersal phase, females exhibited a 4.7‐fold increase in use of small‐sized (<50 g) prey during the time kits are learning to hunt. Male overall diet and predation patterns appear to fit an optimal foraging strategy that is influenced primarily by prey profitability and abundance, with males preying primarily on medium‐sized prey, closest to meeting their lower energetic needs. In contrast, females appear to fit the predictions for a central place forager that is also influenced by prey profitability, but also the increased energetic and maternal demands of denning, leading them to use larger prey than males over the phases of the denning period when kits are growing. We hypothesize that switching to smaller prey is related to females assisting their kits in developing hunting skills and experience that may increase their chances of survival once they disperse.
Post‐natal development of the retinal and cerebellar projections onto the lateral suprasylvian area in the cat.

1. Post‐natal development of the retinal and cerebellar projections onto the medial bank of the lateral suprasylvian visual area was examined by using the field potential method and, additionally, by the orthograde horseradish peroxidase method. 2. Optic nerve stimulation elicited a surface‐positive, depth‐negative field potential in the medial bank of the lateral suprasylvian area of adult cats. By contrast, in kittens younger than 3 weeks old, a surface‐negative, depth‐positive field potential was evoked. The response grew adult‐like by 1 month of age. Corticocortical response, activated by stimulation of cortical areas 17 and 18, underwent a similar developmental change. 3. Cerebellar stimulation evoked a surface‐negative, depth‐positive wave from birth up to adulthood. Thalamocortical afferents from the ventroanterior and ventrolateral nuclei of the thalamus to the medial bank of the lateral suprasylvian area, which is presumed to be responsible for this cerebellar response, terminate mostly in layer I in both new‐born kittens and adult cats. 4. The present results, and our previous morphological findings on the projections from the extrageniculate visual thalamus and visual cortical areas 17 and 18 onto the medial bank of the lateral suprasylvian area, were correlated with reference to the maturation of the neuronal circuit in the cortex.
Analysis of Temperature-Dependent Electrical Characteristics of n-ZnO Nanowires (NWs)/p-Si Heterojunction Diodes

This paper presents the electrical characteristics of n-zinc oxide (ZnO) nanowires (NWs)/p-Si (100) heterojunction diodes fabricated by the oxidation of thermally deposited metallic Zn on Al:ZnO-coated p-Si 〈1 0 0〉 substrates. The electrical parameters of the n-ZnO NWs/p-Si diodes have been estimated by using the room temperature capacitance-voltage (C-V) and temperature-dependent current-voltage (I-V) characteristics of the heterojunction. The carrier concentration of the ZnO NW film and the barrier height of the diode estimated from the C-V characteristics at room temperature are 1.54 × 10 15 cm -3 and 0.75 eV, respectively. The thermionic emission model was used to analyze the temperature-dependent measured I-V characteristics to estimate the parameters of the diode. The estimated values of the barrier height and ideality factor at room temperature were 0.715 eV and 2.13, respectively. The spatial barrier inhomogeneity was included in the aforementioned analysis by assuming a Gaussian distribution for the barrier height at the n-ZnO NWs/p-Si heterojunction. The Richardson constant A* of ZnO was found to be increased from a relatively low value of 9.75 ×10 - 8 A ·cm - 2 ·K - 2 to a more realistic value of 49A ·cm - 2 ·K - 2 after incorporating the barrier inhomogeneity phenomenon in the aforementioned analysis.
Vaccination with Different M2e Epitope Densities Confers Partial Protection against H5N1 Influenza A Virus Challenge in Chickens

Objective: Currently, research is focused on universal influenza vaccines based on various ectodomains of the influenza matrix protein 2 (M2e). Such vaccines are tested mostly using mouse-adapted influenza viruses and in mouse or ferret models. The aim of this study was to investigate in a chicken model the protective efficacy of vaccines based on avian-type M2e at different epitope densities. Methods: On the basis of the optimized avian-type M2e gene, recombinant plasmids that contained tandem copies of different M2e were constructed and expressed in Escherichia coli. The expression and immunogenicity of the proteins were confirmed by SDS-PAGE and Western blot, as well as immunofluorescence assay and enzyme-linked immunosorbent assay. Animals were immunized with fusion proteins emulsified with an appropriate adjuvant and then infected with highly pathogenic influenza virus of A/chicken/Guangdong/04 (H5N1). Antibody levels, survival rate and weight loss were investigated. Results: Multiple copies of M2e were highly expressed; higher epitope density engendered better protection but there was not a linear increase. Among the fusion proteins, the MBP-3·M2e fusion protein showed the best protective efficacy. Conclusions: This study has provided evidence that the immune response to avian-type M2e-based subunit vaccines was greater in chickens than that in mice. In addition, higher M2e epitope density can yield better protection, but not in a linear fashion.
Face Recognition, Musical Appraisal, and Emotional Crossmodal Bias

Recent research on the crossmodal integration of visual and auditory perception suggests that evaluations of emotional information in one sensory modality may tend toward the emotional value generated in another sensory modality. This implies that the emotions elicited by musical stimuli can influence the perception of emotional stimuli presented in other sensory modalities, through a top-down process. The aim of this work was to investigate how crossmodal perceptual processing influences emotional face recognition and how potential modulation of this processing induced by music could be influenced by the subject's musical competence. We investigated how emotional face recognition processing could be modulated by listening to music and how this modulation varies according to the subjective emotional salience of the music and the listener's musical competence. The sample consisted of 24 participants: 12 professional musicians and 12 university students (non-musicians). Participants performed an emotional go/no-go task whilst listening to music by Albeniz, Chopin, or Mozart. The target stimuli were emotionally neutral facial expressions. We examined the N170 Event-Related Potential (ERP) and behavioral responses (i.e., motor reaction time to target recognition and musical emotional judgment). A linear mixed-effects model and a decision-tree learning technique were applied to N170 amplitudes and latencies. The main findings of the study were that musicians' behavioral responses and N170 is more affected by the emotional value of music administered in the emotional go/no-go task and this bias is also apparent in responses to the non-target emotional face. This suggests that emotional information, coming from multiple sensory channels, activates a crossmodal integration process that depends upon the stimuli emotional salience and the listener's appraisal.
Pulmonary vein isolation with high-intensity focused ultrasound: results from the HIFU 12F study.

AIMS High-intensity focused ultrasound (HIFU) applied via a balloon catheter (BC) is a novel technology for simplified pulmonary vein isolation (PVI). Safety and efficacy of the third generation HIFU-BC were assessed. METHODS AND RESULTS In 22 patients (10 male, mean age 65 +/- 6 years) with paroxysmal atrial fibrillation (PAF), a PVI during real-time PV spike registration and oesophageal temperature measurement was attempted. In 15 patients, a steerable sheath was used along with the HIFU-BC. In 67 of 83 PVs (81%), PVI was achieved exclusively using HIFU. Using the steerable sheath, the acute PVI rate rose from 50% (10/20 PVs) to 90% (57/63 PVs). In the latter, PVI was achieved with a single HIFU application in 60% (38/63 PVs) and mean sonication time of 11 +/- 7 s. The mean procedure time was 166 +/- 74 min including 58 +/- 25 min of HIFU-BC left atrial indwelling time. In four patients, peri-procedural complications occurred (one transient ischaemic attack, one phrenic nerve palsy, and two vascular access complications). During a median follow-up of 342 days (range 272-378 days), 71% patients remained free of any AF/AT recurrence without antiarrhythmic drugs after a single procedure. CONCLUSION The novel defocused 12F HIFU-BC used in conjunction with a steerable sheath allows for very rapid PVI in patients with PAF. The enthusiasm for rapid PVI is still dampened by the potential risk of collateral damage.
Advancing patient-centered care for structurally vulnerable drug-using populations: a qualitative study of the perspectives of people who use drugs regarding the potential integration of harm reduction interventions into hospitals.

AIMS To explore the perspectives of structurally vulnerable people who use drugs (PWUD) regarding: (1) the potential integration of harm reduction interventions (e.g. supervised drug consumption services, opioid-assisted treatment) into hospitals; and (2) the implications of these interventions for patient-centered care, hospital outcomes and drug-related risks and harms. DESIGN Semi-structured qualitative interviews. SETTING Vancouver, Canada. PARTICIPANTS Thirty structurally vulnerable PWUD who had been discharged from hospital against medical advice within the past 2 years, and hospitalized multiple times over the past 5 years. MEASUREMENTS Semi-structured interview guide including questions to elicit perspectives on hospital-based harm reduction interventions. FINDINGS Participant accounts highlighted that hospital-based harm reduction interventions would promote patient-centered care by: (1) prioritizing hospital care access and risk reduction over the enforcement of abstinence-based drug policies; (2) increasing responsiveness to subjective health needs (e.g. pain and withdrawal symptoms); and (3) fostering 'culturally safe' care. CONCLUSIONS Hospital-based harm reduction interventions for people who use drugs, such as supervised drug consumption services and opioid-assisted treatment, can potentially improve hospital care retention, promote patient-centered care and reduce adverse health outcomes among people who use drugs.