Miracle-dz/UniFAR-FTUs huggingface dataset:
Kinetic analysis of the influenza A virus HA/NA balance reveals contribution of NA to virus-receptor binding and NA-dependent rolling on receptor-containing surfaces

Interactions of influenza A virus (IAV) with sialic acid (SIA) receptors determine viral fitness and host tropism. Binding to mucus decoy receptors and receptors on epithelial host cells is determined by a receptor-binding hemagglutinin (HA), a receptor-destroying neuraminidase (NA) and a complex in vivo receptor-repertoire. The crucial but poorly understood dynamics of these multivalent virus-receptor interactions cannot be properly analyzed using equilibrium binding models and endpoint binding assays. In this study, the use of biolayer interferometric analysis revealed the virtually irreversible nature of IAV binding to surfaces coated with synthetic sialosides or engineered sialoglycoproteins in the absence of NA activity. In addition to HA, NA was shown to be able to contribute to the initial binding rate while catalytically active. Virus-receptor binding in turn contributed to receptor cleavage by NA. Multiple low-affinity HA-SIA interactions resulted in overall extremely high avidity but also permitted a dynamic binding mode, in which NA activity was driving rolling of virus particles over the receptor-surface. Virus dissociation only took place after receptor density of the complete receptor-surface was sufficiently decreased due to NA activity of rolling IAV particles. The results indicate that in vivo IAV particles, after landing on the mucus layer, reside continuously in a receptor-bound state while rolling through the mucus layer and over epithelial cell surfaces driven by the HA-NA-receptor balance. Quantitative BLI analysis enabled functional examination of this balance which governs this dynamic and motile interaction that is expected to be crucial for penetration of the mucus layer and subsequent infection of cells by IAV but likely also by other enveloped viruses carrying a receptor-destroying enzyme in addition to a receptor-binding protein.
On Robustness of Massive MIMO Systems against Passive Eavesdropping under Antenna Selection

In massive MIMO wiretap settings, the base station can significantly suppress eavesdroppers by narrow beamforming toward legitimate terminals. Numerical investigations show that by this approach, secrecy is obtained at no significant cost. We call this property of massive MIMO systems "secrecy for free" and show that it not only holds when all the transmit antennas at the base station are employed, but also when only a single antenna is set active. Using linear precoding, the information leakage to the eavesdroppers can be sufficiently diminished, when the total number of available transmit antennas at the base station grows large, even when only a fixed number of them are selected. This result indicates that passive eavesdropping has no significant impact on massive MIMO systems, regardless of the number of active transmit antennas.
Endogenous glucose production after sequential meals in humans: evidence for more prolonged suppression after ingestion of a second meal.

Single-meal studies have shown that carbohydrate ingestion causes rapid and persistent suppression of endogenous glucose production (EGP). However, little is known about the regulation of EGP under real-life eating patterns in which multiple carbohydrate-containing meals are consumed throughout the day. Therefore, we aimed to characterize the regulation of EGP in response to sequential meals, specifically during the breakfast-lunch transition. Nine healthy individuals (5 men, 4 women; 32 ± 2 yr; 25.0 ± 1.4 kg/m2) ingested two identical mixed meals, each containing 25 g of glucose, separated by 4 h, and EGP was determined by the variable infusion tracer-clamp approach. EGP was rapidly suppressed after both meals, with the pattern and magnitude of suppression being similar over the initial 75-min postmeal period. However, EGP suppression was more transient after breakfast compared with lunch, with EGP returning to basal rates 3 h after breakfast. In contrast, EGP remained in a suppressed state for the entire 4-h postlunch period. This occurred despite each meal eliciting similar plasma glucose and insulin responses. However, there was greater suppression of plasma glucagon levels after lunch, likely contributing to this response. These findings highlight the potential for distinct regulation of EGP with each meal of the day and suggest that EGP may be in a suppressed state for much of the day, since EGP did not return to basal rates even after a lunch meal containing a modest amount of carbohydrate.
Chemically induced inflammation and nerve damage affect the distribution of vasoactive intestinal polypeptide‐like immunoreactive (VIP‐LI) nervous structures in the descending colon of the domestic pig

The enteric nervous system (ENS), situated in the wall of the gastrointestinal tract, regulates the majority of intestinal activities in physiological conditions and during pathological processes. Enteric neurons are diversified in terms of active substance expression. One of the most important neuropeptides within the ENS is vasoactive intestinal polypeptide (VIP). It seems to be one among the important inhibitory peptides in addition to neuropeptide Y (NPY), nitric oxide (NO), and adenosine triphosphate (ATP) of the intestinal motility and secretion, however, many issues connected with distribution and roles of VIP in the large intestine, especially during pathological states, still remain unknown.
Network-Coded Cooperation With Outdated CSI

The impact of outdated channel state information (CSI) on the performance of multiple-source, multiple-relay network-coded cooperation (NCC) with relay selection (RS) is investigated. In RS-based NCC, <inline-formula> <tex-math notation="LaTeX">$K$ </tex-math></inline-formula> relays are selected (from <inline-formula> <tex-math notation="LaTeX">$M$ </tex-math></inline-formula> cooperative relays). Each of these will decode <inline-formula> <tex-math notation="LaTeX">$N>1$ </tex-math></inline-formula> sources’ messages, uses a non-binary network coding based on maximum distance separable codes to form a network-coded packet, and forwards it to the destination. We derive the exact outage probability and simple and accurate outage approximations (asymptotics) at high signal-to-noise ratio. We show that the full diversity gain of <inline-formula> <tex-math notation="LaTeX">$d=M+1$ </tex-math></inline-formula> is only achieved when <inline-formula> <tex-math notation="LaTeX">$K \geq N$ </tex-math></inline-formula> and CSI are perfect. Outdated CSI, however, degrades the system performance, and the asymptotic diversity gain is always equal to <inline-formula> <tex-math notation="LaTeX">$d=K+1$ </tex-math></inline-formula>. We also give simulation results to validate the theoretical study.
Microstructural White Matter Abnormalities in the Dorsal Cingulum of Adolescents with IBS

Abstract Alterations in fractional anisotropy (FA) have been considered to reflect microstructural white matter (WM) changes in disease conditions; however, no study to date has examined WM changes using diffusion tensor imaging (DTI) in adolescents with irritable bowel syndrome (IBS). The objective of the present study was two-fold: (1) to determine whether differences in FA, and other non-FA metrics, were present in adolescents with IBS compared to healthy controls using whole-brain, region of interest (ROI)-restricted tract-based spatial statistics (TBSS) and canonical ROI DTI analyses for the cingulum bundle, and (2) to determine whether these metrics were related to clinical measures of disease duration and pain intensity in the IBS group. A total of 16 adolescents with a Rome III diagnosis of IBS (females = 12; mean age = 16.29, age range: 11.96–18.5 years) and 16 age- and gender-matched healthy controls (females = 12; mean age = 16.24; age range: 11.71–20.32 years) participated in this study. Diffusion-weighted images were acquired using a Siemens 3-T Trio Tim Syngo MRI scanner with a 32-channel head coil. The ROI-restricted TBSS and canonical ROI-based DTI analyses revealed that adolescents with IBS showed decreased FA in the right dorsal cingulum bundle compared to controls. No relationship between FA and disease severity measures was found. Microstructural WM alterations in the right dorsal cingulum bundle in adolescents with IBS may reflect a premorbid brain state or the emergence of a disease-driven process that results from complex changes in pain- and affect-related processing via spinothalamic and corticolimbic pathways.
Conduction processes in gas-insulated HVDC equipment: from saturated ion currents to micro-discharges

Charge generation in gas-insulated high-voltage direct current (HVDC) equipment has been investigated since surface charge accumulation was detected as the main challenge to insulation coordination. Over the years, researchers have made enormous progress using continuous ion current measurements or surface charging experiments with model spacers downscaled from gas-insulated components. Whereas the low sensitivity of the former method limits its measurement range, the latter method is timeconsuming and demands comprehensive modeling. In this paper, more than 3,000 hours of surface charge measurements are presented, covering the full range of charge generation processes from ion currents owing to natural ionization to the inception of micro-discharges. In accordance with gas-insulated equipment, Al2O3- filled epoxy resin was used for the solids, and sulfur hexafluoride (SF6) was used for the gaseous insulation. It has been demonstrated that the ion pair generation from natural ionization in the gas is significantly influenced by the surrounding solid materials, and therefore, scales with the insulation volume. From the onset of micro-discharges, sharp and locally limited charge patterns are formed, as surface conductivity does not appear to influence the investigated charge distributions. Whereas the primary charge dissipation from surface areas centered at the distribution could be explained in terms of the gas volume size, the discharge currents could not be reproduced with common ion-current models. At low electric fields, the observed charge decay indicates the presence of low-field conduction processes that cannot be explained by charge generation from natural ionization in the gas.
Early start of the West Nile fever transmission season 2018 in Europe

In Europe, surveillance indicates that the 2018 West Nile fever transmission season started earlier than in previous years and with a steeper increase of locally-acquired human infections. Between 2014 and 2017, European Union/European Economic Area (EU/EEA) and EU enlargement countries notified five to 25 cases in weeks 25 to 31 compared with 168 cases in 2018. Clinicians and public health authorities should be alerted to ensure timely implementation of prevention measures including blood safety measures.
More is not always better: An experimental individual-level validation of the randomized response technique and the crosswise model

Social desirability and the fear of sanctions can deter survey respondents from responding truthfully to sensitive questions. Self-reports on norm breaking behavior such as shoplifting, non-voting, or tax evasion may thus be subject to considerable misreporting. To mitigate such response bias, various indirect question techniques, such as the randomized response technique (RRT), have been proposed. We evaluate the viability of several popular variants of the RRT, including the recently proposed crosswise-model RRT, by comparing respondents’ self-reports on cheating in dice games to actual cheating behavior, thereby distinguishing between false negatives (underreporting) and false positives (overreporting). The study has been implemented as an online survey on Amazon Mechanical Turk (N = 6, 505). Our results from two validation designs indicate that the forced-response RRT and the unrelated-question RRT, as implemented in our survey, fail to reduce the level of misreporting compared to conventional direct questioning. For the crosswise-model RRT we do observe a reduction of false negatives. At the same time, however, there is a non-ignorable increase in false positives; a flaw that previous evaluation studies relying on comparative or aggregate-level validation could not detect. Overall, none of the evaluated indirect techniques outperformed conventional direct questioning. Furthermore, our study demonstrates the importance of identifying false negatives as well as false positives to avoid false conclusions about the validity of indirect sensitive question techniques.
Low-Cost IoT: A Holistic Approach

The key factors for a successful smart-city project are its initial cost and its scalability. The initial cost depends on several inter-related aspects that cannot be designed and optimized separately. After the pilot deployment, scaling-up takes place only if the cost remains affordable: an initial financial support may induce dependencies from technologies that become unsustainable in the long period. In addition, the initial adoption of an emerging technology that fails to affirm may jeopardize investment return. This paper investigates a smart-village use case, the success of which strongly depends on the initial cost and scalability, exploring a low-cost way for Internet of Things (IoT). We propose a simple conceptual framework for cost evaluation, and we verify its effectiveness with an exhaustive use case: a prototype sensor designed and tested with its surrounding eco-system. Using experimental results, we can estimate both performance and cost for a pilot system made of fifty sensors deployed in an urban area. We show that such cost grows linearly with system size, taking advantage of widely adopted technologies. The code and the design of the prototype are available, so that all steps are reproducible.
Primary biocompatibility tests of poly(lactide-co-glycolide)-(poly-L-orithine/fucoidan) core–shell nanocarriers

Layer-by-layer (LbL) self-assembly is the technology used in intermolecular static electricity, hydrogen bonds, covalent bonds and other polymer interactions during film assembling. This technology has been widely studied in the drug carrier field. Given their use in drug delivery systems, the biocompatibility of these potential compounds should be addressed. In this work, the primary biocompatibility of poly(lactide-co-glycolide)-(poly-L-orithine/fucoidan) [PLGA-(PLO/fucoidan)] core–shell nanoparticles (NPs) was investigated. Atomic force microscopy revealed the PLGA-(PLO/Fucoidan)4 NPs to be spherical, with a uniform size distribution and a smooth surface, and the NPs were stable in physiological saline. The residual amount of methylene chloride was further determined by headspace gas chromatography, in which the organic solvent can be volatilized during preparation. Furthermore, cell viability, acridine orange/ethidium bromide staining, haemolysis and mouse systemic toxicity were all assessed to show that PLGA-(PLO/fucoidan)4 NPs were biocompatible with cells and mice. Therefore, these NPs are expected to have potential applications in future drug delivery systems.
Multiscaled Fusion of Deep Convolutional Neural Networks for Screening Atrial Fibrillation From Single Lead Short ECG Recordings

Atrial fibrillation (AF) is one of the most common sustained chronic cardiac arrhythmia in elderly population, associated with a high mortality and morbidity in stroke, heart failure, coronary artery disease, systemic thromboembolism, etc. The early detection of AF is necessary for averting the possibility of disability or mortality. However, AF detection remains problematic due to its episodic pattern. In this paper, a multiscaled fusion of deep convolutional neural network (MS-CNN) is proposed to screen out AF recordings from single lead short electrocardiogram (ECG) recordings. The MS-CNN employs the architecture of two-stream convolutional networks with different filter sizes to capture features of different scales. The experimental results show that the proposed MS-CNN achieves 96.99% of classification accuracy on ECG recordings cropped/padded to 5 s. Especially, the best classification accuracy, 98.13%, is obtained on ECG recordings of 20 s. Compared with artificial neural network, shallow single-stream CNN, and VisualGeometry group network, the MS-CNN can achieve the better classification performance. Meanwhile, visualization of the learned features from the MS-CNN demonstrates its superiority in extracting linear separable ECG features without hand-craft feature engineering. The excellent AF screening performance of the MS-CNN can satisfy the most elders for daily monitoring with wearable devices.
Learning Linear Transformations for Fast Arbitrary Style Transfer

Given a random pair of images, an arbitrary style transfer method extracts the feel from the reference image to synthesize an output based on the look of the other content image. Recent arbitrary style transfer methods transfer second order statistics from reference image onto content image via a multiplication between content image features and a transformation matrix, which is computed from features with a pre-determined algorithm. These algorithms either require computationally expensive operations, or fail to model the feature covariance and produce artifacts in synthesized images. Generalized from these methods, in this work, we derive the form of transformation matrix theoretically and present an arbitrary style transfer approach that learns the transformation matrix with a feed-forward network. Our algorithm is highly efficient yet allows a flexible combination of multi-level styles while preserving content affinity during style transfer process. We demonstrate the effectiveness of our approach on four tasks: artistic style transfer, video and photo-realistic style transfer as well as domain adaptation, including comparisons with the state-of-the-art methods.
Non-steroidal anti-inflammatory drugs and the risk of out-of-hospital cardiac arrest: a case–control study

Abstract Aims Non-steroidal anti-inflammatory drugs (NSAIDs), particularly selective COX-2 inhibitors, are associated with an increased risk of cardiovascular adverse events. However, the association between these drugs and out-of-hospital cardiac arrest with electrocardiogram-documented ventricular tachycardia/ventricular fibrillation (VT/VF-OHCA) has not been studied yet. This study was aimed to evaluate the association between the use of selective COX-2 inhibitors or conventional NSAIDs and VT/VF-OHCA compared with non-use. Methods and results A case–control study was conducted among 2483 cases with VT/VF-OHCA from the AmsteRdam REsuscitation STudies (ARREST) registry, an ongoing Dutch registry of OHCA, and 10 441 non-VT/VF-OHCA-controls from the Dutch PHARMO Database Network, containing drug dispensing records of community pharmacies, over the period July 2005–December 2011. Up to five controls were matched for age and sex to one case at the date of VT/VF-OHCA (index date). Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by conditional logistic regression analysis. Of the cases, 0.5% was currently exposed at the index date to selective COX-2 inhibitors and 2.5% to conventional NSAIDs. Neither current use of selective COX-2 inhibitors nor conventional NSAIDs were associated with an increased risk of VT/VF-OHCA (adjusted OR 1.11, 95% CI: 0.79–1.56 and adjusted OR 0.97, 95% CI: 0.86–1.10, respectively) compared with non-use. Stratification for VT/VF-OHCA with presence/absence of acute myocardial infarction did not change these results. Conclusion Exposure to selective COX-2 inhibitors or conventional NSAIDs was not associated with an increased risk of VT/VF-OHCA compared with non-use.
Bug Patterns Localization Based on Topic Model for Bugs in Program Loop

Statistic fault localization technique is a popular technique to perform fault localization via comparing the different statistics of failed executions and successful executions. They measure suspiciousness for each program component and rank them descending by their suspiciousness to assist programmers to identify root cause of failures. Unfortunately, since these techniques based on hit-spectrum information, which use binary flags to register whether a program component is covered or not during a program execution, their results are inherently limited, especially when the bug(s) occur in complex program loops. To resolve this issue, we propose a bug patterns localization technique based on topic model for the bugs in complex loops. We also conduct experiments involving 28 program versions contained bugs in program loops in Siemens program suite to compare the effectiveness of the proposed approach against Dstar. The results show that our approach is promising.
Oligodendrocyte precursor survival and differentiation requires chromatin remodeling by Chd7 and Chd8

Significance Oligodendrocyte precursor cells (OPCs) constitute the main proliferative cells in the adult brain and deregulation of OPC proliferation-differentiation balance results in either glioma formation or defective (re)myelination. Mutations in chromatin remodelers CHD7 and CHD8 are the cause of CHARGE syndrome and some autism spectrum disorders (ASD). Here we show that Chd7 protects OPCs from apoptosis by chromatin closing and gene repression of p53, while Chd7 induces chromatin opening and gene activation of OPC-differentiation regulators. Chd7 is, however, dispensable for oligodendrocyte stage progression, consistent with Chd8 compensatory function, as suggested by their common chromatin-binding profiles, including ASD-risk–associated genes. Our results thus involve oligodendroglia in ASD and CHARGE and offer new avenues to understand and modulate CHD7/CHD8 functions in normal and pathological brain development. Oligodendrocyte precursor cells (OPCs) constitute the main proliferative cells in the adult brain, and deregulation of OPC proliferation-differentiation balance results in either glioma formation or defective adaptive (re)myelination. OPC differentiation requires significant genetic reprogramming, implicating chromatin remodeling. Mounting evidence indicates that chromatin remodelers play important roles during normal development and their mutations are associated with neurodevelopmental defects, with CHD7 haploinsuficiency being the cause of CHARGE syndrome and CHD8 being one of the strongest autism spectrum disorder (ASD) high-risk–associated genes. Herein, we report on uncharacterized functions of the chromatin remodelers Chd7 and Chd8 in OPCs. Their OPC-chromatin binding profile, combined with transcriptome and chromatin accessibility analyses of Chd7-deleted OPCs, demonstrates that Chd7 protects nonproliferative OPCs from apoptosis by chromatin closing and transcriptional repression of p53. Furthermore, Chd7 controls OPC differentiation through chromatin opening and transcriptional activation of key regulators, including Sox10, Nkx2.2, and Gpr17. However, Chd7 is dispensable for oligodendrocyte stage progression, consistent with Chd8 compensatory function, as suggested by their common chromatin-binding profiles and genetic interaction. Finally, CHD7 and CHD8 bind in OPCs to a majority of ASD risk-associated genes, suggesting an implication of oligodendrocyte lineage cells in ASD neurological defects. Our results thus offer new avenues to understand and modulate the CHD7 and CHD8 functions in normal development and disease.