Miracle-dz/UniFAR-FTUs huggingface dataset:
Bystander signaling between glioma cells and fibroblasts targeted with counted particles

Radiation‐induced bystander effects may play an important role in cancer risks associated with environmental, occupational and medical exposures and they may also present a therapeutic opportunity to modulate the efficacy of radiotherapy. However, the mechanisms underpinning these responses between tumor and normal cells are poorly understood. Using a microbeam, we investigated interactions between T98G malignant glioma cells and AG01522 normal fibroblasts by targeting cells through their nuclei in one population, then detecting cellular responses in the other co‐cultured non‐irradiated population. It was found that when a fraction of cells was individually irradiated with exactly 1 or 5 helium particles (3He2+), the yield of micronuclei (MN) in the non‐irradiated population was significantly increased. This increase was not related to the fraction of cells targeted or the number of particles delivered to those cells. Even when one cell was targeted with a single 3He2+, the induction of MN in the bystander non‐irradiated population could be increased by 79% for AG01522 and 28% for T98G. Furthermore, studies showed that nitric oxide (NO) and reactive oxygen species (ROS) were involved in these bystander responses. Following nuclear irradiation in only 1% of cells, the NO level in the T98G population was increased by 31% and the ROS level in the AG0 population was increased by 18%. Treatment of cultures with 2‐(4‐carboxyphenyl)‐4,4,5,5‐tetramethyl‐imidazoline‐1‐oxyl‐3‐oxide (c‐PTIO), an NO scavenger, abolished the bystander MN induction in non‐irradiated AG01522 cells but only partially in non‐irradiated T98G cells, and this could be eliminated by treatment with either DMSO or antioxidants. Our findings indicate that differential mechanisms involving NO and ROS signaling factors play a role in bystander responses generated from targeted T98G glioma and AG0 fibroblasts, respectively. These bystander interactions suggest that a mechanistic control of the bystander effect could be of benefit to radiotherapy. © 2005 Wiley‐Liss, Inc.
A hypercoagulable state follows orthotopic liver transplantation

Orthotopic liver transplantation may be associated during the postoperative period with hepatic artery thrombosis, a catastrophic occurrence generally necessitating emergency retransplantation. To assess the contribution of the coagulation mechanism to this complication, the levels of procoagulant and anticoagulant proteins were followed in 41 liver transplant patients during the first 10 postoperative days. The mean activities of all procoagulant factors reach normal values on day 1 except for factors V and VII, which achieve normal activity by day 3. Supernormal levels of factór VIII activity and antigen are noted (peak values on day 5 of 334% ± 113% and 481% ± 260%, respectively). The anticoagulant proteins show delayed recovery, with deficient antithrombin III levels seen in 81% of patients on day 3 and 57% on day 5. Similarly, proteins C and S are subnormal in 24% and 21%, respectively on day 3, and 20% and 10%, respectively, on day 5. During this period, elevated levels of thrombin/antithrombin complexes are encountered, reflecting in vivo activation of the coagulation mechanism. Activated thrombin is, therefore, being generated at a time when a decrease in the major regulatory anticoagulant proteins exists. These data suggest an imbalance between the hemostatic and thrombotic mechanisms and indicate a sustained prothrombotic state that may contribute to the risk for hepatic artery thrombosis. Using a regimen of low‐dose heparin and fresh frozen plasma infusion, no thromboses have been seen in 65 consecutive liver transplants. (HEPATOLOGY 1990;12:553–558).
WOMEN , GIRLS , AND STRUCTURAL VIOLENCE : A GLOBAL ANALYSIS

Given that the natural birth ratio is 106 males to 100 females, and that females tend to have longer life expectancies, it is shocking to learn that “more than 100 million girls and women are missing” (Sen, 1990, p. 61). Birth ratios of males and females would predict that there should be more women living today, particularly in Asia (Bunch, 1995). But in some parts of the world, men outnumber women by 20:1 (United Nations, 1996). To comprehend this staggering problem, 100 million “missing” women is equivalent to the total number of war-related deaths of 100 Pol Pot–initiated famines and massacres in Cambodia (1975 to 1978), 200 of the genocidal civil wars recently witnessed in Rwanda (1994 to 1995), and 2.5 times the deaths of World War II (1939 to 1945) (Sivard, 1996). But how many have even noticed, let alone demanded accountability for, this killing of girls and women? A primary reason for this disregard is that much of the violence
Psychological Trait Resilience Within Ecological Systems Theory: The Resilient Systems Scales

ABSTRACT This project describes the development of the Resilient Systems Scales, created to address conceptual and methodological ambiguities in assessing the ecological systems model of resilience. Across a number of samples (total N = 986), our findings suggest that the Resilient Systems Scales show equivalence to a previously reported assessment (Maltby, Day, & Hall, 2015) in demonstrating the same factor structure, adequate intercorrelation between the 2 measures of resilience, and equivalent associations with personality and well-being. The findings also suggest that the Resilient Systems Scales demonstrate adequate test–retest reliability, compare well with other extant measures of resilience in predicting well-being, and map, to varying degrees, onto positive expression of several cognitive, social, and emotional traits. The findings suggest that the new measure can be used alongside existing measures of resilience, or singly, to assess positive life outcomes within psychology research.
Total and soluble fluoride content in commercial dentifrices in Chile

Abstract Objective. Regardless of the total amount of fluoride (F) in a dentifrice, only the soluble fraction presents anti-caries activity and its concentration must be above 1000 ppm. No information regarding the quality of F in Chilean toothpastes is available. The aim of this study, therefore, was to determine the F concentration present in commercially-available toothpastes in Chile. Materials and methods: Eighteen toothpastes of five different brands were tested, each purchased from three different chain drugstores in Chile. For each toothpaste, total, total soluble and ionic F concentrations were assessed and expressed as ppm of F (μg F/g). Results. Among the dentifrices evaluated, 77.8% contained NaF (sodium fluoride), 16.7% MFP (monofluorphosphate) and 5.6% both types of F salts; 77.2% of the dentifrices showed similar total F content to that declared by the manufacturer in the labeling with only five with lower concentration than expected, beyond a 5% disagreement tolerance range. Total soluble F ranged from 958.8–1496.3 ppm (mean ± SD: 1270.5 ± 183.7). Five toothpastes showed lower total soluble F than total F. Only one toothpaste showed soluble salt levels below the recommended dose for this type of dentifrice. Conclusion: Most Chilean dentifrices evaluated contained soluble F within the range of 1000–1500 ppm and, therefore, may have anti-caries activity.
Response of Alveolar Macrophages from Inducible Nitric Oxide Synthase Knockout or Wild-Type Mice to an in Vitro Lipopolysaccharide or Silica Exposure

The role of nitric oxide (NO) in pulmonary disease has been controversial with both antiinflammatory (scavenging radicals and inhibiting NF-êB activation) and proinflammatory (forming highly reactive peroxynitrite and augmenting NF-êB activation by inflammatory agents) actions reported. Therefore, a study has been initiated to determine whether deletion of the inducible nitric oxide synthase (iNOS) gene in the C57BL/6J mouse alters the pulmonary macrophage response to lipopolysaccharide (LPS) or silica. The objective of the initial phase of this study was to determine the difference in responsiveness of alveolar macrophages (AMs), harvested from naive wild-type (WT) or iNOS knockout (iNOS KO) mice, to an in vitro LPS or silica exposure. Primary AMs were obtained by bronchoalveolar lavage (BAL) from age- and weightmatched iNOS KO and WT mice. The cells were treated with interferon-gamma (IFN-ã) (50 U/ml), IFN-ã (50 U/ml) + LPS (1 g/ml), LPS (0.01-100 g/ml), or silica (25-250 g/ml). The following parameters were measured: nitrate and nitrite (NOx), tumor necrosis factor-á (TNF-á), macrophage inflammatory protein-2 (MIP-2), intracellular generation of the reactive oxygen species (ROS) hydrogen peroxide (H2O2) and superoxide (O ” . 2 ), and basal (unstimulated) total antioxidant capacity. Data show a significant increase in NOx production upon exposure to IFN-ã - LPS in the WT but not iNOS KO AMs. NOx production by iNOS KO or WT AMs was not altered by in vitro exposure to LPS or silica alone. LPS, but not silica, induced TNF-á and MIP-2 production in both iNOS KO and WT AMs. Statistical analysis of concentrationresponse curves found a significant tendency for greater mediator production in the iNOS KO versus WT AMs. Basal intracellular production of H2O2 and O ” . 2 was significantly greater in the iNOS KO compared to WT AMs. In contrast, LPS- (10 g/ml) or silica- (100 g/ml) stimulated intracellular oxidant production was lower in iNOS KO AMs, but overall (basal + stimulated) inflammatory capacity was similar between the cell types. The basal total antioxidant production of the iNOS KO AMs was approximately twofold higher than the WT AMs. In conclusion, certain compensatory changes appear to occur in AMs from iNOS KO mice. In response to the inability to induce NO production, iNOS KO AMs exhibit significantly higher basal generation of H2O2 and O ” . 2 as well as higher total antioxidant levels. In addition, LPSinduced TNF-á and MIP-2 production tend to be higher in AMs from iNOS KO mice. Such compensatory changes in the AM response may affect the response of iNOS KO mice to inflammatory exposures.
The effect of calcium-deficient diet on the reproductive organs of the hen (Gallus domesticus).

Abstract INTRODUCTION THE restriction of dietary calcium affects profoundly the reproductive performance of the laying hen. Earlier investigations on the subject are reviewed by Mehring and Titus (1964). When hens are fed on a calcium-deficient diet they normally stop laying after 10–14 days (Taylor et al., 1962). The cessation of egg production is due to the failure of gonadotrophin secretion by the anterior pituitary which causes the bird to stop ovulating. If exogenous gonadotrophins are administrated to calcium-deficient hens, they continue ovulating and laying soft shelled eggs. It is speculated that the hypothalamic stimulation of the pituitary is reduced by the fall in the diffusible calcium level of the blood of calcium-depleted hens. (Taylor, 1965). The egg shell calcium content declines progressively from about 2 gm. per egg shell to about 1 gm. per egg shell during the first 7 days in hens placed on calciumrestricted diets. The recovery of…
phylogeny and phylogenetic taxonomy of dipsacales, with special reference to sinadoxa and tetradoxa (adoxaceae)

To further clarify phylogenetic relationships within Dipsacales,we analyzed new and previously published rbcL sequences, alone and in combination with morphological data. We also examined relationships within Adoxaceae using rbcL and nuclear ribosomal internal transcribed spacer (ITS) sequences. We conclude from these analyses that Dipsacales comprise two major lineages:Adoxaceae and Caprifoliaceae (sensu Judd et al.,1994), which both contain elements of traditional Caprifoliaceae. Within Adoxaceae, the following relationships are strongly supported: (Viburnum (Sambucus (Sinadoxa (Tetradoxa, Adoxa)))). Combined analyses of C ap ri foliaceae yield the fo l l ow i n g : ( C ap ri folieae (Diervilleae (Linnaeeae (Morinaceae (Dipsacaceae (Triplostegia, Valerianaceae)))))). On the basis of these results we provide phylogenetic definitions for the names of several major clades. Within Adoxaceae, Adoxina refers to the clade including Sinadoxa, Tetradoxa, and Adoxa. This lineage is marked by herbaceous habit, reduction in the number of perianth parts,nectaries of multicellular hairs on the perianth,and bifid stamens. The clade including Morinaceae, Valerianaceae, Triplostegia, and Dipsacaceae is here named Valerina. Probable synapomorphies include herbaceousness,presence of an epicalyx (lost or modified in Valerianaceae), reduced endosperm,and distinctive chemistry, including production of monoterpenoids. The clade containing Valerina plus Linnaeeae we name Linnina. This lineage is distinguished by reduction to four (or fewer) stamens, by abortion of two of the three carpels,and possibly by supernumerary inflorescences bracts.
The Demise of Novell Netware—Did Perceptions Related to Network Administration Play a Role?

In this study the authors examine some possible reasons for Novell Netware's fall from dominance in the 1990s to its near disappearance. The authors examine the role of the external variables security, productivity, and sharing on an administrator's perceptions and intention to use the software within the Technology Acceptance Model (TAM). The results show that difficulty in administration was not responsible for Netware's demise; on the contrary, Netware's environment resulted in a positive intention to use it.
The Association between Asthma and Absenteeism among Working Adults in the United States: Results from the 2008 Medical Expenditure Panel Survey

Objective. The purpose of this study was to evaluate the association between asthma status and the occurrence and length of work absences among the US working adults. Methods. A cross-sectional study was conducted using the 2008 Medical Expenditure Panel Survey (MEPS). Employed respondents between ages 18 and 55 years were included. The association between asthma status (whether respondents have asthma or not) and occurrence of absences and the length of time per absence was evaluated using a two-part model. A multivariate logistic regression as the first part of the model was to estimate the probability of being absent from work at least once during the observation period as a function of asthma status. A multivariate negative binomial regression as the second part of the model was used to assess whether the length of each absence from work was associated with asthma status among respondents who reported at least one absence from work. Sociodemographic, socioeconomic, employment-related, health status, and comorbidity variables were included in each model as covariates. Results. Of 12,161 respondents, 8.2% reported having asthma, which accounted for 10.4 million working adults in the United States in 2008. Employed adults with asthma were more likely to report having at least one absence from work compared to those without asthma in bivariate analyses (26.2% vs. 16.2%, p < .01). After adjusting for the number of comorbid chronic conditions and other covariates, there was no significant difference between having asthma and absenteeism among respondents (odds ratio (OR) = 1.31, 95% confidence interval (CI) = 0.99–1.72, rate ratio (RR) = 1.25, 95% CI = 0.91–1.72). Conclusions. Overall burden of illness as measured by comorbidity indices and perceived health status, but not asthma alone, contributes to absenteeism as well as the number of days off during each occurrence among employed people. It is important for health services researchers to consider overall burden of illness when examining the association between a general outcome such as absence from work and specific conditions such as asthma.
Incidental and context-responsive activation of structure- and function-based action features during object identification.

Previous studies suggest that action representations are activated during object processing, even when task-irrelevant. In addition, there is evidence that lexical-semantic context may affect such activation during object processing. Finally, prior work from our laboratory and others indicates that function-based ("use") and structure-based ("move") action subtypes may differ in their activation characteristics. Most studies assessing such effects, however, have required manual object-relevant motor responses, thereby plausibly influencing the activation of action representations. The present work uses eyetracking and a Visual World Paradigm task without object-relevant actions to assess the time course of activation of action representations, as well as their responsiveness to lexical-semantic context. In two experiments, participants heard a target word and selected its referent from an array of four objects. Gaze fixations on nontarget objects signal activation of features shared between targets and nontargets. The experiments assessed activation of structure-based (Experiment 1) or function-based (Experiment 2) distractors, using neutral sentences ("S/he saw the.") or sentences with a relevant action verb (Experiment 1: "S/he picked up the."; Experiment 2: "S/he used the."). We observed task-irrelevant activations of action information in both experiments. In neutral contexts, structure-based activation was relatively faster-rising but more transient than function-based activation. Additionally, action verb contexts reliably modified patterns of activation in both Experiments. These data provide fine-grained information about the dynamics of activation of function-based and structure-based actions in neutral and action-relevant contexts, in support of the "Two Action System" model of object and action processing (e.g., Buxbaum & Kalénine, 2010).
Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40.

S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base pairs, which are at or below the limit of detectability by conventional electron microscopic analysis of heteroduplex DNAs, have been located in this way. To map a deletion, a mixture of unit length, linear DNA, prepared from the SV40 deletion mutant and its wild-type parent, are denatured and reannealed to form heteroduplexes. S1 nuclease can cut such heteroduplexes at the nonbase-paired region to produce fragments whose lengths correspond to the position of the deletion. Similarly, specific fragments are produced when S1 nuclease cleaves a heteroduplex formed from the DNAs of SV40 temperature-sensitive mutants and either their revertants or wild-type parents. Thus, the positions of the nonhomology between these DNAs can be determined.
Interplay of Aro80 and GATA activators in regulation of genes for catabolism of aromatic amino acids in Saccharomyces cerevisiae

Aro80, a member of the Zn2Cys6 family proteins, activates expression of the ARO9 and ARO10 genes involved in catabolism of aromatic amino acids in response to aromatic amino acids that act as inducers. ARO9 and ARO10 are also under the control of nitrogen catabolite repression, but the direct roles for GATA factors, Gat1 and Gln3, in this regulation have not yet been elucidated. Here, we demonstrate that Aro80 is constitutively bound to its target promoters and activated by inducers at the level of transactivation. Although Aro80 also binds to its own promoter, ARO80 expression is induced only by rapamycin, but not by tryptophan. We show that Aro80 is absolutely required for Gat1 binding to the ARO9, ARO10 and ARO80 promoters upon rapamycin treatment. Gln3 binding to these promoters shows a partial requirement for Aro80. Rapamycin‐dependent Gat1 and Gln3 binding to the Aro80 target promoters is not affected by tryptophan availability, suggesting that transactivation activity of Aro80 is not necessary for the recruitment of GATA factors. Rapamycin‐dependent induction of Aro80 target genes also requires PP2A phosphatase complex, but not Sit4 phosphatase, acting downstream of TORC1.
In vivo measurement of polyethylene wear in cementless total hip arthroplasty.

PURPOSE OF THE STUDY Recent years have seen an increase in cementless total hip endoprosthesis (THP) implantations. Easy radiological measurement of wear in cups coated with X-ray dense material, such as metal or ceramics, is not applicable. An alternative technique has thus been developed to radiographically measure wear on cementless spherical implants. MATERIAL AND METHODS The authors present a method in which the center of the head of the prosthesis relative to the center of the cup was defined by graphical extrapolation. Changes between the two centers on subsequent images allowed the estimation of wear. Data from 148 randomly selected patients with cemented THP were compared with that from 50 with cementless THP (head diameter always 32 mm). RESULTS Within the cemented group metal heads showed significant more wear than ceramic heads (1.244 mm vs. 0.504 mm). However, calculating the wear rate per year showed no significant difference. No significant differences were observed comparing cemented and cementless group. A comparison of the own results with those of the literature revealed analogous values. DISCUSSION Data of the cementless group were comparable with those obtained from conventional wear measurements on cemented hip cups thus validating the technique. CONCLUSION The method offers the potential to define wear of metal cups with a polyethylene inlay or with X-ray dense components such as ceramic or metal inlays.
Distinct regulation of the interleukin-1 and interleukin-6 response elements of the rat haptoglobin gene in rat and human hepatoma cells

The transcription rate of the haptoglobin (Hp) gene is stimulated by interleukin-1 (IL-1), IL-6, and dexamethasone in rat hepatoma (H-35) cells. To identify the cis-acting regulatory elements responsive to these hormones, various lengths of 5' Hp gene-flanking regions, including the promoter, were inserted into chloramphenicol acetyltransferase gene expression vectors and transiently introduced into H-35 cells. The first 4 kb of 5' region mediated a severalfold increase in expression after treatment with IL-6 and dexamethasone. No response to IL-1 was detectable. When, however, upstream sequences were deleted to position -165 relative to the transcription start site, a significant stimulation by IL-1 was gained without appreciably affecting the IL-6 response. With the apparent removal of an inhibitory sequence, the promoter-proximal 165-bp region also displayed a severalfold enhanced response to the combination of dexamethasone, IL-1, and IL-6. The sequence from -165 to -147, termed the A-element, was found to be crucial for all hormone regulatory functions. Two copies of the A-element linked to a heterologous promoter responded to the three hormones, but to a lesser degree than in the Hp gene promoter context. The regulatory elements of the rat Hp gene were similarly active in human hepatoma cells. Optimal regulation by IL-6 in HepG2 cells was, however, independent of the A-element. The A-element functioned in these cells exclusively as an IL-1 response sequence. The results suggest that genomic sequences upstream of the rat Hp gene suppress the regulation by specific cytokines more prominently in transient expression assays than in the normal chromosomal context. Moreover, the functional comparison indicated that specific regulatory regions of the rat Hp gene do not function identically in different hepatic cell types.