Miracle-dz/UniFAR-FTUs huggingface dataset:
Enterovirus as trigger of coeliac disease: nested case-control study within prospective birth cohort

Abstract Objective To determine whether infection with human enterovirus or adenovirus, both common intestinal viruses, predicts development of coeliac disease. Design Case-control study nested within Norwegian birth cohort recruited between 2001 and 2007 and followed to September 2016. Setting Norwegian population. Participants Children carrying the HLA genotype DR4-DQ8/DR3-DQ2 conferring increased risk of coeliac disease. Exposures Enterovirus and adenovirus detected using real time polymerase chain reaction in monthly stool samples from age 3 to 36 months. Main outcome measure Coeliac disease diagnosed according to standard criteria. Coeliac disease antibodies were tested in blood samples taken at age 3, 6, 9, and 12 months and then annually. Adjusted odds ratios from mixed effects logistic regression model were used to assess the relation between viral infections before development of coeliac disease antibodies and coeliac disease. Results Among 220 children, and after a mean of 9.9 (SD 1.6) years, 25 children were diagnosed as having coeliac disease after screening and were matched to two controls each. Enterovirus was found in 370 (17%) of 2135 samples and was significantly more frequent in samples collected before development of coeliac disease antibodies in cases than in controls (adjusted odds ratio 1.49, 95% confidence interval 1.07 to 2.06; P=0.02). The association was restricted to infections after introduction of gluten. High quantity samples (>100 000 copies/μL) (adjusted odds ratio 2.11, 1.24 to 3.60; P=0.01) and long lasting infections (>2 months) (2.16, 1.16 to 4.04; P=0.02) gave higher risk estimates. Both the commonly detected enterovirus species Enterovirus A and Enterovirus B were significantly associated with coeliac disease. The association was not found for infections during or after development of coeliac disease antibodies. Adenovirus was not associated with coeliac disease. Conclusions In this longitudinal study, a higher frequency of enterovirus, but not adenovirus, during early childhood was associated with later coeliac disease. The finding adds new information on the role of viral infections in the aetiology of coeliac disease.
Skill acquisition as a function of age, hand and task difficulty: Interactions between cognition and action

Some activities can be meaningfully dichotomised as ‘cognitive’ or ‘sensorimotor’ in nature—but many cannot. This has radical implications for understanding activity limitation in disability. For example, older adults take longer to learn the serial order of a complex sequence but also exhibit slower, more variable and inaccurate motor performance. So is their impaired skill acquisition a cognitive or motor deficit? We modelled sequence learning as a process involving a limited capacity buffer (working memory), where reduced performance restricts the number of elements that can be stored. To test this model, we examined the relationship between motor performance and sequence learning. Experiment 1 established that older adults were worse at learning the serial order of a complex sequence. Experiment 2 found that participants showed impaired sequence learning when the non-preferred hand was used. Experiment 3 confirmed that serial order learning is impaired when motor demands increase (as the model predicted). These results can be captured by reinforcement learning frameworks which suggest sequence learning will be constrained both by an individual’s sensorimotor ability and cognitive capacity.
Pre‐exposure prophylaxis initiation and adherence among Black men who have sex with men (MSM) in three US cities: results from the HPTN 073 study

Randomized clinical trials have demonstrated the efficacy of antiretroviral pre‐exposure prophylaxis (PrEP) in preventing HIV acquisition among men who have sex with men (MSM). However, limited research has examined initiation and adherence to PrEP among Black MSM (BMSM) in the United States (US) who are disproportionately represented among newly HIV infected and late to care individuals. This research reports on the HIV Prevention Trials Network 073 (HPTN 073) study aimed to examine PrEP initiation, utilization and adherence among Black MSM utilizing the theoretically principled, culturally informed and client‐centered care coordination (C4) model.
Do professional facial image comparison training courses work?

Facial image comparison practitioners compare images of unfamiliar faces and decide whether or not they show the same person. Given the importance of these decisions for national security and criminal investigations, practitioners attend training courses to improve their face identification ability. However, these courses have not been empirically validated so it is unknown if they improve accuracy. Here, we review the content of eleven professional training courses offered to staff at national security, police, intelligence, passport issuance, immigration and border control agencies around the world. All reviewed courses include basic training in facial anatomy and prescribe facial feature (or ‘morphological’) comparison. Next, we evaluate the effectiveness of four representative courses by comparing face identification accuracy before and after training in novices (n = 152) and practitioners (n = 236). We find very strong evidence that short (1-hour and half-day) professional training courses do not improve identification accuracy, despite 93% of trainees believing their performance had improved. We find some evidence of improvement in a 3-day training course designed to introduce trainees to the unique feature-by-feature comparison strategy used by facial examiners in forensic settings. However, observed improvements are small, inconsistent across tests, and training did not produce the qualitative changes associated with examiners’ expertise. Future research should test the benefits of longer examination-focussed training courses and incorporate longitudinal approaches to track improvements caused by mentoring and deliberate practice. In the absence of evidence that training is effective, we advise agencies to explore alternative evidence-based strategies for improving the accuracy of face identification decisions.
For Whom Is Anti-Bullying Intervention Most Effective? The Role of Temperament

Studying moderators of the effects of anti-bullying universal interventions is essential to elucidate what works for whom and to tailor more intensive, selective, and indicated programs which meet the needs of non-responders. The present study investigated whether early adolescents’ temperament—effortful control (EC), negative emotionality (NE), and positive emotionality (PE)—moderates the effects of the KiVa anti-bullying program. The sample consisted of 13 schools, with 1051 sixth-grade early adolescents (mean age = 10.93; SD = 0.501), randomly assigned to the KiVa intervention (seven schools; n = 536) or to the control condition (six schools; n = 516). Adolescents reported bullying and victimization before the intervention (pre-test) and after (post-test). Temperament was assessed by a self-report pre-test. Findings showed that EC and NE moderated intervention effects on bullying, indicating that subgroups with high levels of EC, and with low and medium levels of NE were those who benefited most from the intervention. The low-EC subgroup showed a lower increase compared to the control condition, with a considerable effect size. Conversely, the high-NE subgroup did not show any positive effects compared to the control group. Regarding victimization, findings showed that early adolescents with high and medium levels of PE were the subgroups who benefited the most from the intervention, whereas the low-PE subgroup was the most resistant. The present study confirms the relevance of considering temperament as a moderator of intervention effects, since interventions tailored to early adolescents with specific traits might yield larger effects.
3D SSY Estimate of EPFM Constraint Parameter under Biaxial Loading for Sensor Structure Design†

Conventional sensor structure design and related fracture mechanics analysis are based on the single J-integral parameter approach of elastic-plastic fracture mechanics (EPFM). Under low crack constraint cases, the EPFM one-parameter approach generally gives a stress overestimate, which results in a great cost waste of labor and sensor components. The J-A two-parameter approach overcomes this limitation. To enable the extensive application of the J-A approach on theoretical research and sensor engineering problem, under small scale yielding (SSY) conditions, the authors developed an estimate method to conveniently and quickly obtain the constraint (second) parameter A values directly from T-stress. Practical engineering application of sensor structure analysis and design focuses on three-dimensional (3D) structures with biaxial external loading, while the estimate method was developed based on two-dimensional (2D) plain strain condition with uniaxial loading. In the current work, the estimate method was successfully extended to a 3D structure with biaxial loading cases, which is appropriate for practical sensor design. The estimate method extension and validation process was implemented through a thin 3D single edge cracked plate (SECP) specimen. The process implementation was completed in two specified planes of 3D SECP along model thickness. A wide range of material and geometrical properties were applied for the extension and validation process, with material hardening exponent value 3, 5 and 10, and crack length ratio 0.1, 0.3 and 0.7.
Analysis of Obesity among Malaysian University Students: A Combination Study with the Application of Bayesian Structural Equation Modelling and Pearson Correlation

In obesity modelling studies, researchers have been seeking to identify the effective indicators of obesity by using appropriate statistical or mathematical techniques. The main objective of the present study is addressed in three stages. First, a new framework for modelling obesity in university students is introduced. The second stage involves data analysis based on Bayesian Structural Equation Modelling (BSEM) for estimating the Body Mass Index (BMI) (representative of the obesity level) of students at three university levels: Bachelor, Master and PhD. In the third stage, the highest significant correlation is determined between the BMI and other variables in the research model that were found significant through the second phase. The data for this study were collected from students at selected Malaysian universities. The results indicate that unhealthy food intake (fast food and soft drinks), social media use and stress exhibit the highest weightage contributing to overweight and obesity issues for Malaysian university students.
Dynamics of ACTH-Mediated Regulation of Gene Transcription in ATC1 and ATC7 Adrenal Zona Fasciculata Cell Lines

Abstract We tested the hypothesis that mouse ATC1 and ATC7 cells, the first adrenocortical cell lines to exhibit a complete zona fasciculata (ZF) cell phenotype, respond to dynamic ACTH stimulation in a similar manner as the adrenal gland in vivo. Exploiting our previous in vivo observations that gene transcription within the steroidogenic pathway is dynamically regulated in response to a pulse of ACTH, we exposed ATC1 and ATC7 cells to various patterns of ACTH, including pulsatile and constant, and measured the transcriptional activation of this pathway. We show that pulses of ACTH administered to ATC7 cells can reliably stimulate a pulsatile pattern of transcriptional activity that is comparable to that observed in adrenal ZF cells in vivo. Hourly pulses of ACTH stimulate dynamic increases in CREB phosphorylation (pCREB) and transcription of genes involved in critical steps of steroidogenesis including signal transduction (e.g., MRAP), cholesterol delivery (e.g., StAR), and steroid biosynthesis (e.g., CYP11A1), as well as those relating to transcriptional regulation of steroidogenic factors (e.g., SF-1 and Nur-77). In contrast, constant ACTH stimulation results in a prolonged and exaggerated pCREB and steroidogenic gene transcriptional response. We also show that when a large dose of ACTH (100 nM) is applied after these treatment regimens, a significant increase in steroidogenic transcriptional responsiveness is achieved only in cells that have been exposed to pulsatile, rather than constant, ACTH. Our data support our in vivo observations that pulsatile ACTH is important for the optimal transcriptional responsiveness of the adrenal. Importantly, our data suggest that ATC7 cells respond to dynamic ACTH stimulation.
Interleukin-13 maintains the stemness of conjunctival epithelial cell cultures prepared from human limbal explants

To use human limbal explants as an alternative source for generating conjunctival epithelium and to determine the effect of interleukin-13 (IL-13) on goblet cell number, mucin expression, and stemness. Human limbal explants prepared from 17 corneoscleral rims were cultured with or without IL-13 (IL-13+ and IL-13-, respectively) and followed up to passage 2 (primary culture [P0]–P2). Cells were characterized by alcian blue/periodic acid–Schiff (AB/PAS) staining (goblet cells); immunofluorescent staining for p63α (progenitor cells), Ki-67 (proliferation), MUC5AC (mucin, goblet cells), and keratin 7 (K7, conjunctival epithelial and goblet cells); and by quantitative real-time polymerase chain reaction for expression of the p63α (TP63), MUC5AC, MUC4 (conjunctival mucins), K3, K12 (corneal epithelial cells), and K7 genes. Clonogenic ability was determined by colony-forming efficiency (CFE) assay. Using limbal explants, we generated epithelium with conjunctival phenotype and high viability in P0, P1, and P2 cultures under IL-13+ and IL-13- conditions, i.e., epithelium with strong K7 positivity, high K7 and MUC4 expression and the presence of goblet cells (AB/PAS and MUC5AC positivity; MUC5AC expression). p63α positivity was similar in IL-13+ and IL-13- cultures and was decreased in P2 cultures; however, there was increased TP63 expression in the presence of IL-13 (especially in the P1 cultures). Similarly, IL-13 increased proliferative activity in P1 cultures and significantly promoted P0 and P1 culture CFE. IL-13 did not increase goblet cell number in the P0–P2 cultures, nor did it influence MUC5AC and MUC4 expression. By harvesting unattached cells on day 1 of P1 we obtained goblet cell rich subpopulation showing AB/PAS, MUC5AC, and K7 positivity, but with no growth potential. In conclusion, limbal explants were successfully used to develop conjunctival epithelium with the presence of putative stem and goblet cells and with the ability to preserve the stemness of P0 and P1 cultures under IL-13 influence.
Analytical investigation of convective heat transfer of a longitudinal fin with temperature-dependent thermal conductivity, heat transfer coefficient and heat generation

In this article, the heat transfer through a longitudinal fin is studied. The heat transfer coefficient, thermal conductivity and heat generation are variables and supposed to be temperature-dependent. The temperature distribution in fin with longitudinal rectangular profile was carried out by using the differential transformation method (DTM) which is an analytical solution technique. For validation of the analytical solution, the heat equation is solved numerically. The temperature distribution is shown for different values of the embedding parameters. The DTM results indicate that the fin tip temperature increases with an increase in the heat generation gradient. Results reveal that DTM is very effective and convenient. Comparison of the results (DTM and numerical) was shown that the analytical method and numerical data are in a good agreement with each other. Key words: Fins, temperature dependent thermal properties, heat generation, analytical solutions, differential transformation method (DTM).
Acute and Chronic Effects of Cocaine on Cardiovascular Health

Cardiac complications resulting from cocaine use have been extensively studied because of the complicated pathophysiological mechanisms. This study aims to review the underlying cellular and molecular mechanisms of acute and chronic effects of cocaine on the cardiovascular system with a specific focus on human studies. Studies have consistently reported the acute effects of cocaine on the heart (e.g., electrocardiographic abnormalities, acute hypertension, arrhythmia, and acute myocardial infarction) through multifactorial mechanisms. However, variable results have been reported for the chronic effects of cocaine. Some studies found no association of cocaine use with coronary artery disease (CAD), while others reported its association with subclinical coronary atherosclerosis. These inconsistent findings might be due to the heterogeneity of study subjects with regard to cardiac risk. After cocaine use, populations at high risk for CAD experienced coronary atherosclerosis whereas those at low risk did not experience CAD, suggesting that the chronic effects of cocaine were more likely to be prominent among individuals with higher CAD risk. Studies also suggested that risky behaviors and cardiovascular risks may affect the association between cocaine use and mortality. Our study findings highlight the need for education regarding the deleterious effects of cocaine, and access to interventions for cocaine abusers.
Promoting Healthcare Sustainability in Developing Countries: Analysis of Knowledge Management Drivers in Public and Private Hospitals of Pakistan

Investing in a sustainable future has no alternative; the healthcare sector in developing countries has failed to achieve sustainability objectives. Knowledge management (KM) is a concrete application of sustainability in healthcare, as organizations (hospitals) that manage their knowledge assets will gain sustainable competitive advantage. Several organizations in developed countries are moving towards the adoption of knowledge management so that they can manage their knowledge well and improve their performance. Due to the effective implementation of KM in developed countries, developing countries are also considering adopting KM in their healthcare. In this study, an attempt has been made to identify the drivers of KM adoption in public and private hospitals of Pakistan. With the help of an extensive literature review and expert opinion, the drivers were identified and a hierarchical structure was developed. Nineteen drivers were identified and screened out by experts. The experts identified the contextual relationships between the drivers during a brainstorming session. The hierarchical model of the drivers for KM in the healthcare of Pakistan was eventually developed using interpretive structural modeling (ISM). The structure has 10 levels, in which “developed competitive advantage” formed the foundation of the structure and “job creation” and “improvement in the reputation of healthcare” formed the topmost level. The “Matrices d’Impacts Croises Multiplication Appliqué a un Classement” (MICMAC) analysis classified the drivers by categorizing them according to their driving and dependence powers. One driver is identified as autonomous, six drivers as dependent, seven drivers as linkage, and five drivers as independent. The analysis of KM drivers will provide a good understanding of the interdependence and interactions between them and support the effect adoption of KM in developing countries especially in Pakistan.
Efficacy of Gum Arabic as an esculent film on shelf life extension of tomato (Solanum lycopersicum L) fruit

Effect of Gum Arabic (GA) as a non-toxic outer layer to prolong the shelf-life of tomato was carried out in the present study. Tomatoes were coated with 0% (Control) (A), 5% w/v (B), 10% w/v (C), 15% w/v (D) and 20% w/v (E) GA and stored at ambient (33°C; 70%). The polygalacturonase and β-galacturonase activities were carried out. Weight loss, titratable acidity, pH, lycopene, β-Carotene and antioxidant property of the fruits were determined at day 0, 5, 10, 15 and 20 during the experimental duration. Results indicated that polygalacturonase activity ranged from 0.753-1.138 unit/mg, β-galacturonase activity ranged from 1.55-2.78 x 103 miller units, β-carotene ranged from 1.78-23.89 mg/g, antioxidant property ranged from 66.26-192. 12μg/g, titratable acidity was from 0.595-0.95% while the lycopene ranged from 0.77-17.62 mg/g. These results showed that control had significant rapid weight loss with faster rate of softening while the tomatoes coated with GA demonstrated a significant (p<0.05) deferment in ripening as well as maintained its nutritional quality up to 20 days. This indicates that GA may be utilized as an edible coating for tomato. Key words: Edible film, Gum Arabic, ripening, shelf life.
Use of healthcare services at the end of life in decedents compared to their surviving counterparts: A case-control study among adults born before 1946 in Friuli Venezia Giulia

Background There is a heterogeneous literature on healthcare utilization patterns at the end of life. The objective of this study is to examine the impact of closeness to death on the utilization of acute hospital-based healthcare services and some primary healthcare services and compare differences in gender, age groups and major causes of death disease specific mortality. Methods A matched case-control study, nested in a cohort of 411,812 subjects, linked to administrative databases was conducted. All subjects were residents in the Friuli Venezia Giulia Region (Italy), born before 1946, alive in January 2000 and were followed up to December 2014. Overall, 158,571 decedents/cases were matched by gender and year of birth to one control, alive at least one year after their matched case’s death (index-date). Hospital admissions, emergency department visits, drug prescriptions, specialist visits and laboratory tests that occurred 365 days before death/index-date, have been evaluated. Odds Ratios (ORs) for healthcare utilization were estimated through conditional regression models, further adjusted for Charlson Comorbidity Index and stratified by gender, age groups and major causes of death. Results Decedents were significantly more likely of having at least one hospital admission (OR 7.0, 6.9–7.1), emergency department visit (OR 5.2, 5.1–5.3), drug prescription (OR 2.8, 2.7–2.9), specialist visit (OR 1.4, 1.4–1.4) and laboratory test (OR 2.7, 2.6–2.7) than their matched surviving counterparts. The ORs were generally lower in the oldest age group (95+) than in the youngest (55–74). Healthcare utilization did not vary by sex, but was higher in subjects who died of cancer. Conclusion Closeness to death appeared to be strongly associated with healthcare utilization in adult/elderly subjects. The risk seems to be greater among younger age groups than older ones, especially for acute based services. Reducing acute healthcare at the EOL represents an important issue to improve the quality of life in proximity to death.