Miracle-dz/UniFAR-FTUs huggingface dataset:
Histone Variant H2A.Z Functions in Sister Chromatid Cohesion in Saccharomyces cerevisiae

ABSTRACT H2A.Z is a highly conserved variant of histone H2A with well-characterized roles in transcriptional regulation. We previously reported that H2A.Z and Mcd1, a subunit of the cohesin complex, regulate the establishment of transcriptional silencing at telomeres in Saccharomyces cerevisiae and that H2A.Z broadly dissociated from chromatin during the anaphase-to-telophase transition, coincident with the dissociation of Mcd1 from chromosomes and dissolution of cohesion. In this study, we show that depletion of H2A.Z causes precocious loss of sister chromatid cohesion in yeast without loss of Mcd1 from chromosomes. H2A.Z is deposited into chromatin by the SWR1 complex and is subject to acetylation of its four N-terminal tail lysine residues by the NuA4 and SAGA histone acetyltransferase complexes. We found that cells compromised for function of the SWR1 complex were defective in cohesion, as were cells expressing a form of H2A.Z not subject to acetylation. Finally, inactivation of H2A.Z in metaphase-blocked cells led immediately to cohesion defects, suggesting a direct role for H2A.Z in the maintenance of sister chromatid cohesion.
The Impact of a Computer Network on Pediatric Pain and Anxiety

Abstract This was the third randomized controlled clinical trial (RCCT) of Starbright World (SBW)-a private, interactive computer network for hospitalized children. The present randomized controlled clinical trial (RCCT) consisted of a series of 39 replicated single system designs (SSDs) that compared children's self-reports of pain intensity, pain aversiveness and anxiety in the SBW condition with those in a standard clinical practice condition. Findings from the SSDs were aggregated using meta-analysis. Although children experienced slightly less pain intensity, pain aversiveness, and anxiety in the SBW condition, these results did not achieve statistical significance. The relationship of these findings to the two prior studies of the effectiveness of SBW is discussed.
Evaluation of renal growth by magnetic resonance imaging and computerized tomography volumes.

PURPOSE Magnetic resonance imaging (MRI) and computerized tomography (CT) are commonly used to image complex medical conditions but limited data have been reported concerning normal renal volumetric measurement with these imaging techniques. We examined whether normative renal growth curves could be constructed from data derived from these imaging modalities, and from these curves assessed normal and abnormal renal development. MATERIALS AND METHODS Patients who had undergone prior renal MRI or CT were identified. Total renal volume and renal cortical fraction (CF, cortical/total volume) were calculated, and growth curves were derived. To examine the curve utility for abnormal growth assessment, renal ultrasonography of children with reflux nephropathy was examined, and MRI and radionuclide scans were compared. RESULTS A total of 60 patients 2 months to 39 years old who underwent MRI were included in the growth curve. The CF of the 120 kidneys was 75.8 +/- 4.3% and independent of sex and age. In 19 patients with vesicoureteral reflux 13 kidneys had cortical scarring, and the CF was decreased (p <0.001, 63.65 +/- 5.72%), indicating disproportionate cortical loss. No difference between CF for normal and vesicoureteral reflux unscarred kidneys was found. Differential renal function on radionuclide study correlated highly with MRI renal volume (r = 0.91). CT was performed in 70 children 1 to 15 years old (mean age 7.9) volume correlated with age and renal length, and the left kidney was larger than right kidney on MRI and CT. CONCLUSIONS Normative renal growth curves can be constructed from CT and MRI derived renal volumes. Cortical fraction is consistent, and sex and age independent. In reflux nephropathy the CF is reduced and renal differential function on nuclear scan correlates with MRI derived differential volume. This concept may be useful for predicting abnormal renal growth and differential function.
Synthesis of phosphatidylcholine, a typical eukaryotic phospholipid, is necessary for full virulence of the intracellular bacterial parasite Brucella abortus

Phosphatidylcholine (PC) is a typical eukaryotic phospholipid absent from most prokaryotes. Thus, its presence in some intracellular bacteria is intriguing as it may constitute host mimicry. The role of PC in Brucella abortus was examined by generating mutants in pcs (BApcs) and pmtA (BApmtA), which encode key enzymes of the two bacterial PC biosynthetic routes, the choline and methyl‐transferase pathways. In rich medium, BApcs and the double mutant BApcspmtA but not BApmtA displayed reduced growth, increased phosphatidylethanolamine and no PC, showing that Pcs is essential for PC synthesis under these conditions. In minimal medium, the parental strain, BApcs and BApmtA showed reduced but significant amounts of PC suggesting that PmtA may also be functional. Probing with phage Tb, antibiotics, polycations and serum demonstrated that all mutants had altered envelopes. In macrophages, BApcs and BApcspmtA showed reduced ability to evade fusion with lysosomes and establish a replication niche. In mice, BApcs showed attenuation only at early times after infection, BApmtA at later stages and BApcspmtA throughout. The results suggest that Pcs and PmtA have complementary roles in vivo related to nutrient availability and that PC and the membrane properties that depend on this typical eukaryotic phospholipid are essential for Brucella virulence.
Potent Costimulation of Effector T Lymphocytes by Human Collagen Type I1

Purified, resting peripheral blood T lymphocytes were previously reported to undergo β1 integrin-dependent activation when cultured with anti-CD3 mAb coimmobilized with fibronectin, but not type I collagen. However, the extravascular T cells that encounter immobilized extracellular matrix proteins and are involved in disease pathogenesis have different properties from resting peripheral blood cells. In this study, we confirm that resting CD4+ and CD8+ T cells from peripheral blood are costimulated by immobilized fibronectin, but not type I collagen. In contrast, Ag- or mitogen-stimulated CD4+ and CD8+ T cell lines, used as models of the effector cells involved in disease, are more potently costimulated by type I collagen than fibronectin. The collagen-induced effects are similar in assays with serum-free medium and in more physiological assays in which anti-CD3 mAb is replaced by a threshold concentration of Ag and irradiated autologous PBMC as APC. The responses are β1 integrin dependent and mediated largely by very late Ag (VLA) 1 and 2, as shown by their up-regulation on the T cell lines as compared with freshly purified resting PBL, and by the effects of blocking mAb. Reversed phase HPLC located the major costimulatory sequence(s) in the α1 chain of type I collagen, the structure of which was confirmed by amino acid sequencing. The results demonstrate the potential importance of type I collagen, an abundant extracellular matrix protein, in enhancing the activation of extravascular effector T cells in inflammatory disease, and point to a new immunotherapeutic target.
The Impact of Audit Characteristics on Firm Performance: An Empirical Study from an Emerging Economy

The auditor, an important instrument of corporate governance, ensures the transparency and accountability of the firm to the stakeholders. The objective of this paper is to explore the impact of audit characteristics on firm performance. In this study, external audit quality (BIG4), frequencies of audit committee meetings, and audit committee size are used as the proxies of audit characteristics and firm performance is measured through ROA, profit margin and EPS. A total of 503 firm years are considered as sample size from the listed manufacturing firms of Dhaka Stock Exchange (DSE) during the period of 2013 to 2017 to find out the impact of audit characteristics on firm performance. In this study, multivariate regression analysis is conducted using the pooled OLS method. Moreover, time dummy and lag model of multivariate analysis are also analyzed as robust check. The multivariate regression results find that external audit quality (BIG4) and audit committee size are significantly positively associated with firm performance. This study also finds that there is a significant negative relationship between audit committee meeting and firm performance. This study recommends that the regulatory authority and audit committee should review the frequencies of audit committee meeting to make it more effective to ensure better firm performance.
Does financial volatility help in explaining and predicting economic activity

Driven by the difficulty to predict the last financial crisis and possible distortion of predictive power of the conventional financial indicators on economic activity, this thesis provides insample and out-of-sample analyses whether financial volatility helps in explaining and forecasting economic activity. Several measures of financial volatility were constructed, such as: realized volatility, volatility following a Generalized Autoregressive Conditional Heteroskedasticity (GARCH) process, common long-run component of volatility estimated by Dynamic Factor Model, Principal Component Analysis and cyclical components of financial volatilities filtered out with Baxter-King filter. I find that statistically there are measures of financial volatility that help in explaining economic activity. Moreover, out-of-sample analysis suggests that the model with term-spread and volatility of financial volatility (volatility of valueweighted returns of market portfolio volatility) performs best in forecasting economic activity. The inclusion of a volatility measure reduces the noise in estimated probabilities of expansions and leads to the lowest number of uncertain periods, i.e. periods for which probability of recession is between 16.86% (percentage of recessions in the sample) and 50%, an event that in some studies is already considered as a recession. Thus, a certain financial volatility measure improves forecasts from the conventional financial indicators, especially during less volatile times. Moreover, the most parsimonious measure of volatility predicts business cycles best. On the other hand, industrial production growth seems to be barely affected by financial volatility measures, which tend to be a better predictor for the direction of the future path of the economy than the actual growth rate.
Nicotine‐induced enhancement of synaptic plasticity at CA3–CA1 synapses requires GABAergic interneurons in adult anti‐NGF mice

The hippocampus, a key structure for learning and memory processes, receives an important cholinergic innervation and is densely packed with a variety of nicotinic acetylcholine receptors (nAChRs) localized on principal cells and interneurons. Activation of these receptors by nicotine or endogenously released acetylcholine enhances activity‐dependent synaptic plasticity processes. Deficits in the cholinergic system produce impairment of cognitive functions that are particularly relevant during senescence and in age‐related neurodegenerative pathologies. In particular, Alzheimer's disease (AD) is characterized by a selective loss of cholinergic neurons in the basal forebrain and nAChRs in particular regions controlling memory processes such as the cortex and the hippocampus. Field excitatory postsynaptic potentials were recorded in order to examine whether nicotine was able to regulate induction of long‐term potentiation at CA3–CA1 synapses in hippocampal slices from adult anti‐NGF transgenic mice (AD 11), a comprehensive animal model of AD, in which cholinergic deficits due to nerve growth factor depletion are accompanied by progressive Alzheimer‐like neurodegeneration. Both AD 11 and wild‐type (WT) mice exhibited short‐ and long‐lasting synaptic plasticity processes that were boosted by nicotine. The effects of nicotine on WT and AD 11 mice were mediated by both α7‐ and β2‐containing nAChRs. In the presence of GABAA receptor antagonists, nicotine failed to boost synaptic plasticity in AD 11 but not in WT mice, indicating that in anti‐NGF transgenic mice GABAergic interneurons are able to compensate for the deficit in cholinergic modulation of glutamatergic transmission. This compensation may occur at different levels and may involve the reorganization of the GABAergic circuit. However, patch‐clamp whole‐cell recordings from principal cells failed to reveal any change in spontaneous release of GABA following pressure application of nicotine to nearby GABAergic interneurons. Together, these experiments indicate that in AD 11 mice a rearrangement of the GABAergic circuit can ‘rescue’ nicotine‐induced potentiation of synaptic plasticity. This may be relevant for developing proper therapeutic tools useful for the treatment of AD.
Asymmetry of the Pitch Vestibulo‐Ocular Reflex in Patients with Cerebellar Disease

Abstract: Responses to pitch head impulses were measured in 15 patients with cerebellar degeneration and downbeat nystagmus and in 5 control subjects, using three‐axis search coils. For each subject, response gains were calculated as (1) the ratio of instantaneous vertical eye velocity to pitch head velocity (at 30 ms and at 70 ms into the response), and (2) the ratio of peak eye velocity to peak head velocity. Gains varied more widely among patients. When calculated at 70 ms and using peak values, patients had higher gains for downward pitch, and normal subjects had symmetric gains. At 30 ms, gains were more symmetric in patients, but at that point control subjects actually had lower gains for downward pitch. Thus, the pitch gain ratio (ratio of downward gain to upward gain) was consistently greater in patients than in normal subjects. Because downward impulses were generally faster, eye velocities during downward and upward pitch for equivalent head speeds were also compared. Again, patients had higher gains for downward pitch. These results are consistent with the authors' hypothesis that cerebellar disease results in a higher sensitivity of anterior than posterior semicircular canal pathways, perhaps through loss of inhibition from the flocculus/paraflocculus complex on anterior canal secondary neurons in the vestibular nuclei. The pitch gain asymmetry was larger than, and did not correlate with, the velocity of spontaneous upward drift. This supports the notion that other mechanisms are likely to contribute to downbeat nystagmus in these patients.
Contrast Sensitivity for Letter and Grating Targets under Various Stimulus Conditions

Measurement of visual acuity for letters of different contrasts has been suggested as a clinical way to evaluate contrast sensitivity in patients with vision abnormalities. If variable-contrast letter acuity provides information similar to the contrast sensitivity function (CSF), then comparable effects should be seen in stimulus manipulations which simulate decreased vision. Using both our own and published data, we compared the effects of diffusive blur, dioptric blur, and eccentric viewing on contrast sensitivity for letter and grating targets. A diffuser placed close to the eye reduces contrast sensitivity fairly evenly across all spatial frequencies, with similar results for letters and gratings. However, dioptric blur reduces sensitivity substantially more to letters than to comparably fine gratings. Eccentric viewing also produces a larger sensitivity loss for letters than for gratings. Because some stimulus manipulations produce dissimilar changes in contrast sensitivity for letters and gratings, it is questionable whether the results of one measure can be used to draw inferences about the other. It is proposed that local or relative phase discrimination has an important role in explaining the different responses to letter and grating targets.
Prevalence of Malnutrition and Vitamin A Deficiency in Nigerian Preschool Children Subsisting on High Intakes of Carotenes

The prevalence of malnutrition and vitamin A deficiency was determined in 204 preschool children of both sexes aged 3–57 months. The children were recruited from 2 rural communities of Atakumosa Local Government Area of Osun State in South West Nigeria. Dietary vitamin A intake was estimated from frequency of consumption of locally available vitamin A containing food items. Vitamin A status of the children was assessed from concentration of retinol in plasma. Nutritional status was assessed from height and weight compared with international reference standards. The results indicate widespread malnutrition among the children. The prevalence of stunting (low height for age) was 60.8% while prevalence of wasting (low weight for height) was 7.4% and of underweight (low weight for age) 27.5%. Dietary vitamin A intake appeared to be adequate in the children. Intake of vitamin A is predominantly from plant sources. At least 43% of the children consumed the carotene rich red palm oil 6 or more times per week in contrast to less than 1% who consumed eggs or milk for 6 or more times per week. Vitamin A deficiency was low in the children. Only 11.3% of the children had plasma retinol concentration <0. 70µmol/L. The results indicate that childhood malnutrition of public health magnitude can coexist with adequate dietary vitamin A intakes or vitamin A status.
Assessment of Factors Associated With the Delayed Transfer of Pediatric Trauma Patients: An Emergency Physician Survey

Objectives The purpose of this study was to identify, among emergency department (ED) physicians, the potential barriers impacting the appropriate and timely transfer of injured children to pediatric trauma centers. Methods Surveys assessed pediatric trauma knowledge and experience, transfer and imaging decisions, and perceived barriers to patient transfer. Two scenarios were created; one with a child meeting the state trauma triage criteria and one who did not. In April 2010, 936 surveys were mailed to randomly selected ED physicians. Respondents could answer by mail or online until June 30, 2010. Results A total of 486 surveys were returned, and 109 were excluded, leaving 377 included in the study. A majority reported limited experience in the care of the critically ill child, with 93%, 99%, 99%, and 100% respectively, having performed less than 5 intubations, intraosseous line, central line, or chest tube placements in the last year. In the scenario in which the child met criteria to be transferred, 74% appropriately transferred the patient, whereas in the other scenario, 34% transferred the patient. As much as 56% of the respondents reported they would perform a head computed tomography before transfer, mainly to avoid missed injuries and medicolegal concerns. Among those who would not transfer either patient, 27% reported not having an on-call surgeon at all times. Conclusions Innovative measures should be developed so that ED physicians gain a greater understanding of the proper identification of pediatric patients requiring a timely transfer to a pediatric trauma center.
Salmonella Escape from Antigen Presentation Can Be Overcome by Targeting Bacteria to Fcγ Receptors on Dendritic Cells1

Dendritic cells (DCs) are professional APCs with the unique ability to activate naive T cells, which is required for initiation of the adaptive immune response against pathogens. Therefore, interfering with DC function would be advantageous for pathogen survival and dissemination. In this study we provide evidence suggesting that Salmonella enterica serovar typhimurium, the causative agent of typhoid disease in the mouse, interferes with DC function. Our results indicate that by avoiding lysosomal degradation, S. typhimurium impairs the ability of DCs to present bacterial Ags on MHC class I and II molecules to T cells. This process could correspond to a novel mechanism developed by this pathogen to evade adaptive immunity. In contrast, when S. typhimurium is targeted to FcγRs on DCs by coating bacteria with Salmonella-specific IgG, bacterial Ags are efficiently processed and presented on MHC class I and class II molecules. This enhanced Ag presentation leads to a robust activation of bacteria-specific T cells. Laser confocal microscopy experiments show that virulent S. typhimurium is rerouted to the lysosomal degradation pathway of DCs when internalized through FcγR. These observations are supported by electron microscopy studies demonstrating that internalized S. typhimurium shows degradation signs only when coated with IgG and captured by FcγRs on DCs. Therefore, our data support a potential role for bacteria-specific IgG on the augmentation of Ag processing and presentation by DCs to T cells during the immune response against intracellular bacteria.
Sparse decomposition of convolutional features for scene recognition

Scene recognition is an important and challenging problem in the field of computer vision owing to the variations in the same class and the similarities between different classes. This paper presents a novel approach that learns a reasonable dictionary from convolutional features to effectively describe the distinctive and shared properties in scene images. Substantial convolution operations in Deep Convolutional Neural Networks (DCNN) make the output features have redundant and irrelevant information for scene recognition. Sparse Decomposition of Convolutional Features (SDCF) aims to extract the effective components for scene recognition. Convolutional features are apt to capture the holistic appearance and ignore the partial details because the DCNN is trained by the whole images. So we propose to take advantage of the convolutional features of densely sampled patches to compensate for the lack of the local information. The experimental result shows that our approach outperforms the state-of-the-art results on the benchmark.
Neuropsychological correlates of apathy and depression in patients with dementia

Objective: To investigate the association between apathy and depression, and specific cognitive deficits in AD. Background: Apathy and depression are frequent behavioral disorders in patients with AD. However, the neuropsychological correlates of these disorders have rarely been examined. Methods: A comprehensive neuropsychological and psychiatric evaluation was carried out in 72 patients with AD with apathy and depression, 29 patients with AD with apathy only, 31 patients with AD with depression only, and 52 patients with AD with neither apathy nor depression (control group). Results: Patients with apathy had significantly lower scores on tests of verbal memory, naming, set shifting, and verbal fluency compared with patients without apathy. The association of depression and apathy produced significantly more severe deficits compared with apathy only on a test of abstract thinking. Finally, depression in the absence of apathy was not associated with more severe cognitive impairments compared with the AD control group. Conclusions: Apathy, but not depression, is associated with significantly more severe frontal lobe related cognitive deficits in AD.
The power of connections: Psychological sense of community as a predictor of volunteerism

ABSTRACT Two studies explored psychological antecedents of volunteerism, including several dispositional constructs and psychological sense of community (PSOC). In Study 1, 140 retirees completed measures of empathy, self-esteem, generativity, and PSOC, as well as involvement in volunteer organizations and weekly volunteering hours at two points in time. PSOC predicted concurrent and future volunteerism even after controlling for the other predictors. In Study 2 (n = 427), PSOC and measures of environmental concern and connectedness were used to predict current environmental volunteerism and activism. PSOC was the only measure reliably and uniquely related to these behaviors. Across two different domains and operationalizations of PSOC, the findings support the validity and utility of PSOC for understanding general and issue-specific volunteerism. More generally, they highlight social relationships and psychological connections as potential pathways to volunteerism and social action.