Miracle-dz/UniFAR-FTUs huggingface dataset:
Closing the loop on impulsivity via nucleus accumbens delta-band activity in mice and man

Significance We reveal prominent delta oscillations in the nucleus accumbens preceding food reward in mice and use them to guide responsive neurostimulation to suppress binge-like behavior. Similar electrographic signatures are observed in human nucleus accumbens during reward anticipation as well, suggesting their translational potential in the development of a treatment for loss of impulse control in obesity and perhaps additional brain disorders. Reward hypersensitization is a common feature of neuropsychiatric disorders, manifesting as impulsivity for anticipated incentives. Temporally specific changes in activity within the nucleus accumbens (NAc), which occur during anticipatory periods preceding consummatory behavior, represent a critical opportunity for intervention. However, no available therapy is capable of automatically sensing and therapeutically responding to this vulnerable moment in time when anticipation-related neural signals may be present. To identify translatable biomarkers for an off-the-shelf responsive neurostimulation system, we record local field potentials from the NAc of mice and a human anticipating conventional rewards. We find increased power in 1- to 4-Hz oscillations predominate during reward anticipation, which can effectively trigger neurostimulation that reduces consummatory behavior in mice sensitized to highly palatable food. Similar oscillations are present in human NAc during reward anticipation, highlighting the translational potential of our findings in the development of a treatment for a major unmet need.
ntioxidant properties of Baccharis trimera in the neutrophils of Fisher rats runo

Ethnopharmacological relevance: Baccharis trimera (Less.) (Asteraceae) is a native plant of Brazil. Also known as “carqueja”, it has been popularly used to treat liver diseases, diabetes, as well as digestive disorders. Other studies have described the hepatoprotective, antioxidant and anti-inflammatory activities of the species. Aim of the study: The aim of the present study was to investigate the antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats in both in vitro and in vivo experimental models. Material and methods: In the in vitro assay, the neutrophils of male rats were isolated and incubated with
Adolescents' Anxiety in Dating Situations: The Potential Role of Friends and Romantic Partners

This study examined adolescents' interpersonal functioning, including the qualities of their closest friendships and romantic relationships, as predictors of dating/heterosocial anxiety. An ethnically diverse sample of 781 adolescents (57% girls; ages 15–19 years) completed measures that assessed the number and type of close friends, the presence of a romantic relationship, the qualities of their best same-sex friendship and their romantic relationship (using the Network of Relationships Inventory–Revised), and levels of dating or heterosocial anxiety (using the Dating Anxiety Scale for Adolescents). Most adolescents were romantically involved, and girls were more likely to have a romantic partner than boys. Adolescents with fewer other-sex friends and those with less positive and more negative interactions with their best friends reported high levels of dating anxiety. In addition, adolescents who reported never having a romantic relationship, who did not have a current romantic partner, and who had less positive and more negative interactions with their romantic partners reported higher levels of dating anxiety. Variations were noted for different aspects of dating anxiety. The findings indicate that multiple aspects of adolescents' social relations may be independently and uniquely related to feelings of distress in dating or heterosocial situations. Adolescents' social relationships have the potential to support or interfere with the development of successful romantic relationships.
The SopEΦ Phage Integrates into the ssrA Gene of Salmonella enterica Serovar Typhimurium A36 and Is Closely Related to the Fels-2 Prophage

ABSTRACT Salmonella spp. are enteropathogenic gram-negative bacteria that use a large array of virulence factors to colonize the host, manipulate host cells, and resist the host's defense mechanisms. Even closely related Salmonella strains have different repertoires of virulence factors. Bacteriophages contribute substantially to this diversity. There is increasing evidence that the reassortment of virulence factor repertoires by converting phages like the GIFSY phages and SopEΦ may represent an important mechanism in the adaptation of Salmonella spp. to specific hosts and to the emergence of new epidemic strains. Here, we have analyzed in more detail SopEΦ, a P2-like phage from Salmonella enterica serovar Typhimurium DT204 that encodes the virulence factor SopE. We have cloned and characterized the attachment site (att) of SopEΦ and found that its 47-bp core sequence overlaps the 3′ terminus of the ssrA gene of serovar Typhimurium. Furthermore, we have demonstrated integration of SopEΦ into the cloned attB site of serovar Typhimurium A36. Sequence analysis of the plasmid-borne prophage revealed that SopEΦ is closely related to (60 to 100% identity over 80% of the genome) but clearly distinct from the Fels-2 prophage of serovar Typhimurium LT2 and from P2-like phages in the serovar Typhi CT18 genome. Our results demonstrate that there is considerable variation among the P2-like phages present in closely related Salmonella spp.
Insulin Receptor Substrate Proteins Create a Link between the Tyrosine Phosphorylation Cascade and the Ca2+-ATPases in Muscle and Heart*

Following phosphorylation by the insulin receptor kinase, the insulin receptor substrates (IRS)-1 and IRS-2 bind to and activate several Src homology 2 (SH2) domain proteins. To identify novel proteins that interact with IRS proteins in muscle, a human skeletal muscle cDNA expression library was created in the λEXlox system and probed with baculovirus-produced and tyrosine-phosphorylated human IRS-1. One clone of the 10 clones which was positive through three rounds of screening represented the C terminus of the human homologue of the adult fast twitch skeletal muscle Ca2+-ATPase (SERCA1) including the cytoplasmic tail and part of transmembrane region 10. Western blot analysis of extracts of rat muscle demonstrated co-immunoprecipitation of both IRS-1 and IRS-2 with the skeletal muscle Ca2+-ATPase (SERCA1) and the cardiac muscle isoform (SERCA2). In both cases, injection of insulin stimulated a 2- to 6-fold increase in association of which was maximal within 5 min. In primary cultures of aortic smooth muscle cells and C2C12 cells, the insulin-stimulated interaction between IRS proteins and SERCA1 and -2 was dose-dependent with a maximum induction at 100 nm insulin. This interaction was confirmed in a “pull down” experiment using a glutathioneS-transferase fusion protein containing the C terminus of the human SERCA isoform and phosphorylated IRS-1 in vitroand could be blocked by a FLVRES-like domain peptide present in the human SERCA sequence. Affinity chromatography of phosphopeptide libraries using the glutathione S-transferase fusion protein of the C terminus of SERCA1 indicated a consensus sequence for binding of XpYGSS; this is identical to potential tyrosine phosphorylation sites at position 431 of human IRS-1 and at position 500 of human IRS-2. In streptozotocin diabetic rats the interaction between IRS proteins and SERCA1 in skeletal muscle and SERCA2 in cardiac muscle was significantly reduced. Taken together, these results indicate that the IRS proteins bind to the Ca2+-ATPase of the sarcoplasmic reticulum in an insulin-regulated fashion, thus creating a potential link between the tyrosine phosphorylation cascade and effects of insulin on calcium.
Longitudinal outcome of youth oppositionality: irritable, headstrong, and hurtful behaviors have distinctive predictions.

OBJECTIVE Oppositional behavior in youths is one of the strongest predictors of a wide range of psychiatric disorders. We test the hypothesis that oppositionality encompasses an Irritable, a Headstrong, and a Hurtful dimension, each with distinct predictions. METHOD Longitudinal design combining data from two British national surveys and their respective 3-year follow-ups (N = 7,912). The Developmental and Well-Being Assessment was used to generate DSM-IV diagnoses. RESULTS The Irritable dimension was the sole predictor of emotional disorders at follow-up and was particularly associated with distress disorders (depression and anxiety) rather than fear disorders (phobias, separation anxiety, and panic disorder), both before and after adjustment for baseline psychopathology. The Headstrong dimension was the only predictor of attention-deficit/hyperactivity disorder at follow-up. Both Headstrong and Hurtful predicted conduct disorder, although only the Headstrong dimension did so after adjustment for baseline psychopathology. The Hurtful dimension was the strongest predictor of aggressive conduct disorder symptoms. CONCLUSIONS Our data suggest a developmental model of mental disorder whereby oppositionality is an interim shared manifestation of different dimensions of psychopathology with distinct outcomes.
Thyroid Doppler ultrasonography and resistive index in the evaluation of the need for ablative or antithyroid drug therapy in Graves' hyperthyroidism.

BACKGROUND AND PURPOSE Graves' disease (GD) is the most common hyperthyroid disorder, but the therapeutic strategy for choosing between medical or ablative therapy has not been standardized. Thyroid hypervascularity is an important diagnostic feature in GD. This study collected Doppler ultrasonography data from patients with GD, Hashimoto's thyroiditis, and simple goiter to develop a hemodynamic index for use in the evaluation of when antithyroid drugs (ATDs) should be withdrawn or ablative therapy given in GD. MATERIAL AND METHODS Thyroid Doppler ultrasonography was used to measure the resistive index (RI) and pulsatility index (PI) in various thyroid diseases. We studied 88 patients, including 13 untreated GD patients, 14 euthyroid GD patients after withdrawal of ATDs for more than 12 months, 14 euthyroid GD patients with normal thyroid stimulating hormone (TSH) concentrations after regular ATD treatment for 12 months (well controlled), 16 hyperthyroid GD patients with undetectable TSH concentrations after regular ATD treatment for more than 12 months (poorly controlled), 13 Hashimoto's thyroiditis patients, and 18 patients with simple goiters. RESULTS The PI and RI were significantly different between patients with untreated (median PI/RI 1.36/0.79) or medically well-controlled (median PI/RI 0.66/0.51) GD, but no significant differences in PI and RI were found between patients with untreated or medically poorly controlled (median PI/RI 1.24/0.74) GD. An RI cut-off of at least 0.7 with undetectable TSH was found to be suggestive of the need for ablative therapy in GD patients who had received regular ATD treatment for more than 12 months, because of its statistically high sensitivity and specificity in all untreated and poorly controlled GD patients. An RI of less than 0.6 with normal TSH was suggestive of the need for withdrawal of ATDs in GD patients receiving regular medical treatment, because of its statistically high sensitivity and specificity in all treated euthyroid GD patients. CONCLUSIONS This study has developed an RI-derived hemodynamic index that determines the need for ablative or ATD therapy in patients with GD. A large-scale, prospective study is needed to confirm its clinical value.
Effect of age on bronchodilator response in acute severe asthma treatment.

STUDY OBJECTIVES This study was designed to evaluate the effects of age on bronchodilator response to salbutamol in patients with acute severe asthma in the emergency department. SUBJECTS AND METHODS Sixty-four sequential patients (mean age, 34.2+/-10.7 years) with acute asthma were enrolled in the trial. Using age as a major criterion, we divided the sample in two groups: the young one (age < or = 35 years, n=30) and the older (> 35 years, n=34). All patients were treated with salbutamol delivered with metered-dose inhaler into a spacer device, in a dose of four puffs every 10 min (100 microg per actuation) during 3 h. RESULTS Mean FEV1 improved significantly over baseline values for both groups (p=0.001). At final disposition, the mean percent of predicted FEV1 was 55.1+/-16.3% in the young group and 58.0+/-20.9% in the older group. There were no significant differences between both groups for FEV1 percent response at any point studied. A significant increase in heart rate over baseline was seen in the older group (p=0.001). Older patients also presented a higher incidence in nausea and tremor. Young and older patients with acute asthma achieved equivalent bronchodilation response to salbutamol. CONCLUSIONS We concluded that age is not a predictor of response to beta-agonists.
Urine protein profiling with surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry.

BACKGROUND In the last few years there has been an increasing interest in exploring the human proteome. In particular, efforts have focused on developing strategies to generate reproducible protein maps of normal cells, tissues, and biologic fluids, from which studies can then compare protein expression between different groups (e.g., healthy individuals vs. those with a specific pathologic state). METHODS Various extrinsic factors (instrument settings, matrix composition, urine storage post void, freeze-thaw cycles) and intrinsic factors (blood in urine, urine dilution, first-void vs. midstream urine) were analyzed with respect to their impact on urine protein profiling using surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). RESULTS Extrinsic factors that critically influenced reproducibility and peak detection of urine protein profiling were matrix composition and instrument settings, while freeze-thaw cycles had minimal impact. Midstream urines samples did not undergo changes in their protein profile when stored for three days at 4 degrees C. Intrinsic factors that influenced normal urine protein profiling were blood in the urine and urine dilution. Female first-void urine had a significantly different ratio of proteins present compared to a midstream urine sample. Limitations of the SELDI-TOF-MS technique included ion suppression and quantification of individual proteins when protein composition was complex. CONCLUSION SELDI-TOF-MS offers a unique platform for high throughput urine protein profiling; however, standardization of analysis conditions is critical, and both extrinsic and intrinsic factors must be taken into account for accurate data interpretation.
Women's Motives for Violent and Nonviolent Behaviors in Conflicts

Drawing from past research on women's motives for intimate partner violence (IPV) perpetration, correlates of women's perpetration, and correlates of nonviolent conflict, we created a scale containing 125 possible motives, representing 14 broad domains (e.g., self-defense, retaliation). Participants were an ethnically diverse sample of women who had perpetrated no physical IPV against their current partner (n = 243), threats but not physical IPV (n = 70), nonsevere physical IPV (n = 193), and at least one act of severe (e.g., choke) physical IPV (n = 93). An exploratory factor analysis yielded a seven-factor solution, representing Partners' Negative Behaviors, Increase Intimacy, Personal Problems, Retaliation, Childhood Experiences, Situation/Mood, and Partners' Personal Problems. Differences by women's IPV perpetration and race and/or ethnicity were tested with means representing these seven factors and a computed variable representing self-defense. Although motives differed by perpetration type, main effects for Partners' Negative Behavior, Personal Problems, Retaliation, and Childhood Experiences were modified by interactions, suggesting ethnicity should be considered when developing interventions.
Anisotropic Reentry in the Epicardial Border Zone of Myocardial Infarcts a

In a currently used classification of arrhythmogenic mechanisms, there are two major classes of reentry-anatomical and functional. The former is dependent on anatomically defined reentrant pathways that may course around an obstacle as originally shown in Mines”-* and Garrey’s3 studies on excised rings of cardiac muscle. It is exemplified in the intact heart by bundle branch reentry,4 reentry utilizing an accessory AV pathway in the preexcitation syndrome5 and reentry in the ring of muscle around the tricuspid valve causing atrial flutter.6 On the other hand, functional reentry is dependent on heterogeneities of the electrophysiological properties of the cardiac fibers caused by local differences in the transmembrane action potentials, for example, resting potentials, time course of repolarization, and recovery of excitability. It does not require an anatomically defined reentrant pathway. The mechanism for functional reentry that has been characterized in the most detail is the “leading circle mechanism” that can occur in normal atrial tissue. ’ Recent studies indicate that the anatomy of cardiac muscle may be important for reentry in a way other than simply providing a road over which the reentrant impulse travels. Anatomy may participate in determining functional properties of cardiac muscle, since the structural characteristics of heart muscle can influence both conduction and refractor ine~s. ~. ~ These structural characteristics include the orientation of the myocardial fibers and the way the fibers or bundles of fibers are connected to each other. The influence of structure on functional properties is described by the concept of anisotropy (see paper by Spach et al., this volume).
Development of a fuzzy decision support framework for complex multi‐attribute decision problems: A case study for the selection of skilful basketball players

The selection of skilful players is a complicated process due to the problem criteria consisting of both qualitative and quantitative attributes as well as vague linguistic terms. This study seeks to develop a decision support framework for the selection of candidates eligible to become basketball players through the use of a fuzzy multi‐attribute decision making (MADM) algorithm. The proposed model is based on fuzzy analytic hierarchy process (FAHP) and Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) methods. The model was employed in the Youth and Sports Center of Mugla, Turkey, with the participation of seven junior basketball players aged between 7 and 14. In the present study, physical fitness measurement values and observation values of technical skills were utilized. FAHP was used to determine the weights of the criteria and the observation values of technical skills by decision makers. Physical fitness measurement values were converted to fuzzy values by using a fuzzy set approach. Subsequently, the overall ranking of the candidate players was determined by the TOPSIS method. Results were compared with human experts’ opinions. It is observed that the developed model is more reliable to be used in decision making. The model architecture and experimental results along with illustrative examples are further demonstrated in the study.
Person entity linking in email with NIL detection

For each specific mention of an entity found in a text, the goal of entity linking is to determine whether the referenced entity is present in an existing knowledge base, and if so to determine which KB entity is the correct referent. Entity linking has been well explored for dissemination‐oriented sources such as news stories, blogs, and microblog posts, but the limited work to date on “conversational” sources such as email or text chat has not yet attempted to determine when the referent entity is not in the knowledge base (a task known as “NIL detection”). This article presents a supervised machine learning system for linking named mentions of people in email messages to a collection‐specific knowledge base, and that is also capable of NIL detection. This system learns from manually annotated training examples to leverage a rich set of features. The entity linking accuracy for entities present in the knowledge base is substantially and significantly better than the best previously reported results on the Enron email collection, comparable accuracy is reported for the challenging NIL detection task, and these results are for the first time replicated on a second email collection from a different source with comparable results.
Vaginal douching and reduced fertility.

OBJECTIVES This study investigated douching and reduced fertility. METHODS The monthly probability of conception for douchers and nondouchers was compared in a sample of 840 married, parous women in King County, Washington. Data on the number of months required to conceive were analyzed. RESULTS In comparison with nondouchers, women who douched were 30% less likely to become pregnant each month they attempted pregnancy. This relationship remained after adjustment for covariates, and it could not be explained by women douching for medical reasons. The reduction was not related to the type of douching preparation used. Young women who douched had significantly greater reductions in monthly fertility than older women (50% reduction for women 18 to 24 years old, 29% reduction for women 25 to 29 years old, and 6% reduction for women 30 to 39 years old). CONCLUSIONS Douching was associated with reduced fertility. Further research is needed to determine whether the relationship is casual and, if so, to what extent it is mediated by pelvic infection. In the meantime, women should be informed that douching may have adverse effects.