Miracle-dz/UniFAR-FTUs huggingface dataset:
Investigation of 0.2 W m ¢lterable bacteria from the Western Mediterranean Sea using a molecular approach : dominance of potential starvation forms

Although the existence of 0.2 W m filterable bacteria has been known since the early 80’s, they are not taken into consideration when modeling microbial food webs, due to an overall lack of information concerning this specific size class. According to physiological studies on starvation forms and investigations on small bacterial cells in marine ecosystems, a 0.2 W m filtrate may consist of different phenotypes: starvation forms of typical marine bacteria, ultramicrobacteria or bacterial cells, even larger than 0.2 W m, but flexible enough to pass the nominal filter pore-size. In this pilot study we examined three filtered seawater fractions from the Western Mediterranean Sea (Bay of Calvi, Corsica/France) ^ the total bacterial population, the bacterial fraction above 0.2 W m and the 0.2 W m filtrate ^ to investigate the bacterial community structure of each of those fractions by the molecular approach of denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments. The analysis of the resulting DGGE profiles revealed different patterns of dominant bands for the 0.2 W m filterable and the total bacterial populations within the samples. Additionally the 0.2 W m filterable bacterial compartment exhibited obvious differences in band patterns for winter and summer samples, which were not observed for the total bacterial fraction. According to the current knowledge concerning the status of 0.2 W m filterable bacteria, DGGE patterns indicate that most of the fragments representing 0.2 W m filterable bacteria were rather starvation forms of marine bacteria than ultramicrobacteria. The sequencing of excised and cloned DNA bands of the DGGE profiles characterized the phylogenetic affiliation of the corresponding 0.2 W m filterable bacteria, clustering mainly with known, typical marine isolates of both K -subclass and Q -subclass of the Proteobacteria and the Cytophaga-Flavobacterium-Bacteroides branch. (cid:223) 1999 Published by Elsevier Science B.V. All rights reserved.
Why do consumers buy socially responsible products

This paper describes a study into consumers' reasons for buying socially responsible (SR) products, such as Fair Trade products and organic meat. As opposed to other studies, we use a qualitative approach based on 25 in-depth interviews and include several different products in the research. This leads to several new results, such as: (1) buying SR products is perceived as an imperfect moral duty; (2) low quality of SR products is a dissatisfier, but high quality not a satisfier; (3) the attitude towards SR products is related to the reputation of charitable funds; (4) the demand for SR products is negatively related to the frequency of purchasing SR products; (5) reflection on SR products raises the demand for SR products; (6) consumers that have witnessed the social problems that SR products aim to alleviate purchase more SR products. Finally, we find that the demand for different SR products is correlated: if a consumer buys one SR product, it is more likely that (s)he purchases other SR products as well.
Factors affecting carriage of Neisseria meningitidis among Greek military recruits

SUMMARY Greek military recruits (993) were examined for carriage of meningococci during July 1990. Blood, saliva and throat swab specimens were obtained and each recruit answered a questionnaire providing information on age, education (a measure of socioeconomic level), place of residence, smoking habits and recent infections. The overall carriage rate was 25% but differed between the two camps: 79/432 (18%) in Camp A and 168/561 (30%) in Camp B (P < 0·0005). In Camp B, there were significantly higher proportions of recruits who were non-secretors (P < 0·0005), and/or heavy smokers (P < 0·0005). They were also younger ( < 19 years old) (P < 0·001), and on the whole had fewer years of education (P < 0·0005). By univariate analysis, carriage was significantly associated with smoking. By multiple logistic regression analysis, carriage was associated with smoking (P < 0·001), age (P < 0·01) and the camp in which the recruits were based (P < 0·01). Among recruits in Camp B, 15/38 (40%) of those with recent viral infections were carriers compared with 30% for the camp in general.
Pauline Arnold (1894‐1974): pioneer in market research

Purpose – The purpose of this paper is to provide a biographical sketch of Pauline Arnold focusing on her pioneering contributions to the field of market research.Design/methodology/approach – Archival source material included the Pauline Arnold Collection at the University of Minnesota and the Lucy Sallick Papers including correspondence, unpublished documents, and the transcript of a 1995 oral history interview with Matilda White Riley, who was Pauline Arnold's stepdaughter. Primary historical source material includes the scholarship, both published and unpublished, of the subject. An important primary, published source for this study is the periodical, Market Research, to which Arnold contributed under the auspices of the Market Research Corporation of America from 1934 through to 1938.Findings – Pauline Arnold's contributions to the field of market research are documented.Originality/value – Pauline Arnold has been cited as having made important but neglected contributions to market research, includin.
Torque decomposition and control in an iron core linear permanent magnet motor

This paper concerns the decomposition and control of the torque produced by an iron core linear permanent magnet motor. The proposed method is based on the dq0-decomposition of the three-phase currents using Park's transformation. The torque is decomposed into a reluctance component and two electromagnetic components which depend on the d- and q-axis currents. By curve fitting a harmonic model on finite-element results and measurements, the torque components are modeled as a function of the motor position. To control the torque at any position, the required d- and q-axis currents are solved from the torque model. It is shown that the proposed controller successfully controls the torque in both FE-simulations and during measurements.
Beneficial versus adverse effects of long‐term use of clenbuterol in mdx mice

Long‐term administration of the β2‐adrenergic agonist clenbuterol in mdx mice was used to test the hypothesis that increasing contractile protein content in skeletal muscle will decrease the progression of muscular dystrophy. C57BL/10SNJ (control) and dystrophic (mdx) mice were given clenbuterol (1.0–1.5 mg/kg body weight/day) in the drinking water. Ventilatory function and morphological and functional characteristics of soleus (SOL) and diaphragm (DIA) muscles were evaluated. Clenbuterol administration was associated with increased SOL muscle weight, and SOL muscle weight to body weight ratio in control and mdx mice at both ages. There was a 22% increase in myosin concentration of mdx DIA at 1 year of age, correlating well with increased normalized active tension in mdx DIA at this age. Also, absolute tetanic tension increased in control and mdx SOL with clenbuterol at both ages. Ventilatory function was significantly impaired in mdx mice at both ages and clenbuterol administration did not alleviate this. Clenbuterol treatment was associated with a 30–40% increase in fatigability in DIA and SOL muscles of control and mdx mice at both ages. Furthermore, 1‐year‐old mdx mice receiving clenbuterol exhibited deformities in hindlimbs and spine. These results suggest that long‐term clenbuterol treatment has a positive effect on muscle growth and force generation, but has adverse side effects such as increased muscle fatigability and development of deformities. © 1995 John Wiley & Sons, Inc.
Secondary surgical cytoreduction for advanced ovarian carcinoma.

BACKGROUND We evaluated the effect of adding secondary cytoreductive surgery to postoperative chemotherapy on progression-free survival and overall survival among patients who had advanced ovarian cancer and residual tumor exceeding 1 cm in diameter after primary surgery. METHODS Women were enrolled within six weeks after primary surgery. If, after three cycles of postoperative paclitaxel plus cisplatin, a patient had no evidence of progressive disease, she was randomly assigned to undergo secondary cytoreductive surgery followed by three more cycles of chemotherapy or three more cycles of chemotherapy alone. RESULTS We enrolled 550 women. After completing three cycles of postoperative chemotherapy, 216 eligible patients were randomly assigned to receive secondary surgical cytoreduction followed by chemotherapy and 208 to receive chemotherapy alone. Surgery was declined by or medically contraindicated in 15 patients who were assigned to secondary surgery (7 percent). As of March 2003, 296 patients had died and 82 had progressive disease. The likelihood of progression-free survival in the group assigned to secondary surgery plus chemotherapy, as compared with the chemotherapy-alone group, was 1.07 (95 percent confidence interval, 0.87 to 1.31; P=0.54), and the relative risk of death was 0.99 (95 percent confidence interval, 0.79 to 1.24; P=0.92). CONCLUSIONS For patients with advanced ovarian carcinoma in whom primary cytoreductive surgery was considered to be maximal, the addition of secondary cytoreductive surgery to postoperative chemotherapy with paclitaxel plus cisplatin does not improve progression-free survival or overall survival.
Compliance With Infection Prevention Guidelines By Health Care Workers at Ronald Ross General Hospital Mufulira District

Objective: To determine the level of health-care workers’ compliance with Infection Prevention Guidelines and identify factors that influence compliance at Ronald Ross General Hospital, Mufulira District. Methods: A quantitative study was carried out in 2007. Convenient sampling method was used. Data was obtained using a self administered interview schedule and an observation checklist. A total of 77 health care workers who included Doctors, Registered Midwives and Nurses, Enrolled Midwives and Nurses, clinical Officers, Laboratory Technicians and physiotherapists took part in the study. Additionally, 40 out of the 77 interviewed health workers were observed carrying out at least one procedure requiring compliance with the Infection Prevention (IP) guidelines. Results: The study revealed that, high compliance was associated with inclusion of Guidelines in the Curricular, high knowledge of infection prevention/hospital acquired infections, positive attitude towards infection prevention and availability of materials for infection prevention. The study further reviewed revealed varied levels of compliance on different components of infection prevention. The highest level of compliance (100%) was with single use of needles and syringes while the lowest (35.1) was with decontamination of needles and syringes with 0.5% chlorine solution prior to disposal. Compliance with hand hygiene was moderate (61%). Conclusion: The study findings suggest a need for inclusion of Infection Prevention Guidelines in the health workers’ curricular, provision of in-service training in infection prevention protocols and improvements in the supply of materials for infection prevention.
Evolution of uncontrolled proliferation and the angiogenic switch in cancer.

The major goal of evolutionary oncology is to explain how malignant traits evolve to become cancer ``hallmarks. " One such hallmark---the angiogenic switch---is difficult to explain for the same reason altruism is difficult to explain. An angiogenic clone is vulnerable to ``cheater" lineages that shunt energy from angiogenesis to proliferation, allowing the cheater to outcompete cooperative phenotypes in the environment built by the cooperators. Here we show that cell- or clone-level selection is sufficient to explain the angiogenic switch, but not because of direct selection on angiogenesis factor secretion---angiogenic potential evolves only as a pleiotropic afterthought. We study a multiscale mathematical model that includes an energy management system in an evolving angiogenic tumor. The energy management model makes the counterintuitive prediction that ATP concentration in resting cells increases with increasing ATP hydrolysis, as seen in other theoretical and empirical studies. As a result, increasing ATP hydrolysis for angiogenesis can increase proliferative potential, which is the trait directly under selection. Intriguingly, this energy dynamic allows an evolutionary stable angiogenesis strategy, but this strategy is an evolutionary repeller, leading to runaway selection for extreme vascular hypo- or hyperplasia. The former case yields a tumor-on-a-tumor, or hypertumor, as predicted in other studies, and the latter case may explain vascular hyperplasia evident in certain tumor types.
An in vitro examination of the antioxidant and anti-inflammatory properties of buckwheat honey.

OBJECTIVE Hydroxyl radical and hypochlorite anion formed at the wound site from superoxide anion produced by activated polymorphonuclear neutrophils (PMNs) are considered important factors in impaired wound healing. Superoxide anion may also react with nitric oxide produced by macrophages to form peroxynitrite, a third strong oxidant that damages surrounding tissue. In order to select honey for use in wound-healing products, different samples were compared for their capacity to reduce levels of reactive oxygen species (ROS) in vitro. METHOD Honey samples were tested in assays for inhibition of ROS production by activated human PMNs, antioxidant activity (scavenging of superoxide anion in a cell-free system) and inhibition of human complement (reducing levels of ROS by limiting formation of complement factors that attract and stimulate PMNs). For buckwheat honey (NewYork, US), moisture and free acid content were determined by refractive index measurement and potentiometric titration respectively. Honey constituents other than sugars were investigated by thin layer chromatography, using natural product reagent to detect phenolic compounds. Constituents with antioxidant properties were detected by spraying the chromatogram with DPPH. RESULTS Although most honey samples were shown to be active, significant differences were observed, with the highly active honey exceeding the activities of samples with minor effects by factors of 4 to 30. Most pronounced activities were found for American buckwheat honey from the state of NewYork. Phenolic constituents of buckwheat honey were shown to have antioxidant activity. CONCLUSION As buckwheat honey was most effective in reducing ROS levels, it was selected for use in wound-healing products. The major antioxidant properties in buckwheat honey derive from its phenolic constituents, which are present in relatively large amounts. Its phenolic compounds may also exert antibacterial activity, whereas its low pH and high free acid content may assist wound healing.
Gestural-Sonorous Objects: embodied extensions of Schaeffer's conceptual apparatus

One of the most remarkable achievements of Pierre Schaeffer's musical thought is his proposal of the sonorous object as the focus of research. The sonorous object is a fragment of sound, typically in the range of a few seconds (often even less), perceived as a unit. Sonorous objects are constituted, studied, and evaluated according to various criteria, and sonorous objects that are found suitable are regarded as musical objects that may be used in musical composition. In the selection and qualification of these sonorous objects, we are encouraged to practise what Schaeffer called ‘reduced listening’, meaning disregarding the original context of the sound, including its source and signification, and instead focus our listening on the sonorous features. However, it can be argued that this principle of ‘reduced listening’ is not in conflict with more fundamental principles of embodied cognition, and that the criteria for the constitution, and the various feature qualifications, of sonorous objects can be linked to gestural images. Also, there are several similarities between studying sound and gestures from a phenomenological perspective, and it is suggested that Schaeffer's theoretical concepts may be extended to what is called gestural-sonorous objects.
Changes in physical activity among Brazilian adults over a 5-year period

Background The aim of this study was to document changes in physical activity of Brazilian adults by comparing two surveys carried out 5 years apart. Methods Two population-based cross-sectional surveys were carried out in the city of Pelotas, Brazil, in 2002 and 2007. Their multistage sampling strategies were virtually identical. The first study included 3182 and the second 2986 adults aged 20 years or older. The short version of the International Physical Activity Questionnaire (IPAQ) was used in both surveys, and individuals were classified as insufficiently active if reporting less than 150 min per week, according to a score combining moderate-intensity and vigorous-intensity physical activity. Results Prevalence of insufficient physical activity increased from 41.1% (95% CI 37.4 to 44.9) in 2002 to 52.0% (95% CI 49.1 to 53.8) in 2007. A 70% increase in prevalence of insufficient physical activity (p=0.008) was observed among poor individuals, whereas there was no significant change in the better-off. In contrast to the direct association between insufficient physical activity and socioeconomic level found in 2002, the 2007 survey showed no association. In the 2007 multivariable analysis, insufficient physical activity was directly associated with age and inversely with schooling. Conclusion Effective interventions for the promotion of physical activity are urgently required in order to overcome the decline in physical activity levels in this population, particularly among the poor.
Failure of the Smallpox Vaccine To Develop a Skin Lesion in Vaccinia Virus-Naïve Individuals Is Related to Differences in Antibody Profiles before Vaccination, Not After

ABSTRACT Successful vaccination against smallpox with conventional vaccinia virus is usually determined by the development of a vesicular skin lesion at the site of vaccinia inoculation, called a “take. ” Although previous vaccination is known to be associated with attenuation of the take, the immunology that underlies a no-take in vaccinia-naïve individuals is not well understood. We hypothesized that antibody profiling of individuals before and after receiving vaccinia virus would reveal differences between takes and no-takes that may help better explain the phenomenon. Using vaccinia virus proteome microarrays and recombinant protein enzyme-linked immunosorbent assays (ELISAs), we first examined the antibody response in vaccinia-naïve individuals that failed to take after receiving different doses of the replication-competent DryVax and Aventis Pasteur (APSV) smallpox vaccines. Most that received diluted vaccine failed to respond, although four no-takes receiving diluted vaccine and four receiving undiluted vaccine mounted an antibody response. Interestingly, their antibody profiles were not significantly different from those of controls that did show a take. However, we did find elevated antibody titers in no-takes prior to receiving DryVax that were significantly different from those of takes. Although the sample size studied was small, we conclude the failure to take in responders correlates with preexisting immunity of unknown etiology that may attenuate the skin reaction in a way similar to previous smallpox vaccination.
Repair of porcine articular cartilage defect with a biphasic osteochondral composite

Autologous chondrocyte implantation (ACI) has been recently used to treat cartilage defects. Partly because of the success of mosaicplasty, a procedure that involves the implantation of native osteochondral plugs, it is of potential significance to consider the application of ACI in the form of biphasic osteochondral composites. To test the clinical applicability of such composite construct, we repaired osteochondral defect with ACI at low cell‐seeding density on a biphasic scaffold, and combined graft harvest and implantation in a single surgery. We fabricated a biphasic cylindrical porous plug of DL‐poly‐lactide‐co‐glycolide, with its lower body impregnated with β‐tricalcium phosphate as the osseous phase. Osteochondral defects were surgically created at the weight‐bearing surface of femoral condyles of Lee‐Sung mini‐pigs. Autologous chondrocytes isolated from the cartilage were seeded into the upper, chondral phase of the plug, which was inserted by press‐fitting to fill the defect. Defects treated with cell‐free plugs served as control. Outcome of repair was examined 6 months after surgery. In the osseous phase, the biomaterial retained in the center and cancellous bone formed in the periphery, integrating well with native subchondral bone with extensive remodeling, as depicted on X‐ray roentgenography by higher radiolucency. In the chondral phase, collagen type II immunohistochemistry and Safranin O histological staining showed hyaline cartilage regeneration in the experimental group, whereas only fibrous tissue formed in the control group. On the International Cartilage Repair Society Scale, the experimental group had higher mean scores in surface, matrix, cell distribution, and cell viability than control, but was comparable with the control group in subchondral bone and mineralization. Tensile stress–relaxation behavior determined by uni‐axial indentation test revealed similar creep property between the surface of the experimental specimen and native cartilage, but not the control specimen. Implanted autologous chondrocytes could survive and could yield hyaline‐like cartilage in vivo in the biphasic biomaterial construct. Pre‐seeding of osteogenic cells did not appear to be necessary to regenerate subchondral bone. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:1277–1290, 2007
A Novel Role for an Insect Apolipoprotein (Apolipophorin III) in β-1,3-Glucan Pattern Recognition and Cellular Encapsulation Reactions 1

Lipoproteins and molecules for pattern recognition are centrally important in the innate immune response of both vertebrates and invertebrates. Mammalian apolipoproteins such as apolipoprotein E (apoE) are involved in LPS detoxification, phagocytosis, and possibly pattern recognition. The multifunctional insect protein, apolipophorin III (apoLp-III), is homologous to apoE. In this study we describe novel roles for apoLp-III in pattern recognition and multicellular encapsulation reactions in the innate immune response, which may be of direct relevance to mammalian systems. It is known that apoLp-III stimulates antimicrobial peptide production in insect blood, enhances phagocytosis by insect blood cells (hemocytes), and binds and detoxifies LPS and lipoteichoic acid. In the present study we show that apoLp-III from the greater wax moth, Galleria mellonella, also binds to fungal conidia and β-1,3-glucan and therefore may act as a pattern recognition molecule for multiple microbial and parasitic invaders. This protein also stimulates increases in cellular encapsulation of nonself particles by the blood cells and exerts shorter term, time-dependent, modulatory effects on cell attachment and spreading. All these responses are dose dependent, occur within physiological levels, and, with the notable exception of β-glucan binding, are only observed with the lipid-associated form of apoLp-III. Preliminary studies also established a beneficial role for apoLp-III in the in vivo response to an entomopathogenic fungus. These data suggest a wide range of immune functions for a multiple specificity pattern recognition molecule and may provide a useful model for identifying further potential roles for homologous proteins in mammalian immunology, particularly in terms of fungal infections, pneumoconiosis, and granulomatous reactions.