Miracle-dz/UniFAR-FTUs huggingface dataset:
Neonatal necrotizing enterocolitis: an overview

Purpose of review Necrotizing enterocolitis represents a disease entity that remains quite challenging for neonatologists all around the world, in that its aetiology has yet to be revealed, but it is the cause of death for many premature infants each year, affecting up to 28% of very low birthweight infants. This is an attempt to improve the management of affected babies and stimulate more research concerning new diagnostic tools. Recent findings Current trends in the field of (early) diagnosis, such as: (1) imaging techniques, e.g. contrast radiography, portal vein ultrasonography, magnetic resonance, radionuclide scanning; (2) gastrointestinal tonometry; (3) the detection of biochemical markers, cytokines, growth factors; and (4) the determination of the mean peak hydrogen: carbon dioxide ratio excreted in breath, are only some of those mentioned. Various novel preventive techniques are also presented, among which platelet-activating factor acetyl hydrolase activity enhancement, platelet-activating factor receptor antagonists and probiotics, such as Bifidobacterium infantis and Lactobacillus acidophilus, seem quite promising. Regarding treatment, the use of oxygenated perfluorocarbon has added to the limited alternatives available. These data along with other recent discoveries concerning the risk factors and pathogenesis of this disease create a full picture of the current opinion on this topic. Summary Keeping in mind that the key to confronting such a devastating disorder as necrotizing enterocolitis is early diagnosis and prevention, both clinically applicable and experimental advances are presented with the hope of improving the survival rates of patients affected.
The Motivations, Relationships, and Decision-Making of Western Pennsylvania Public School Board Members

The local control of public schools in the United States provides opportunities for everyday citizens to participate in governance and shape educational programs (Tyack, 2003). This study surveyed the people who volunteered to serve on Western Pennsylvania school boards, specifically examining their motivations for service including the recruitment process, experiences or interactions that motivated board service, interests, and socialization methods once on the board. The study also uncovered information regarding the decision-making processes employed by school board members and their relationships and patterns of communication with others in the school community. Additionally, the study collected information on the challenges of school board leadership and what would help board members in their service. The study found that the majority of respondents were self-motivated to seek election and were interested in giving back to the community, being most often interested in curricular issues or financial issues. Respondents chiefly used resources provided by the district to gather information for decision-making and learned about their roles primarily from other school board members or through state organizations. Respondents regularly communicated with other school board members and the superintendent, primarily in face-to-face conversations
Adolescent Intentional Abuse Ingestions: Overall 10-Year Trends and Regional Variation

Objective Adolescent intentional ingestions remain a significant public health problem in the United States with little research to date on the over-the-counter or prescription medicines that adolescents abuse. These data are important for anticipatory guidance by primary care providers, preventive health, and poison center outreach. Methods This was an observational study using the American Association of Poison Control Centers National Poison Data System. The study population consisted of all cases of patients aged 13 to 19 years from 2004 to 2013 with a coding of “intentional abuse. ” Results There were 95,695 patient calls that were coded for intentional abuse between 2004 and 2013 for adolescents aged 13 to 19 years. The most common agent reportedly ingested in intentional-abuse cases was antihistamine and/or decongestant with dextromethorphan, and this agent remained the most common throughout the 10-year study period. The next 4 most common agents remained similar across the study period as well and included ethanol, benzodiazepines, dextromethorphan alone, and marijuana. These 5 agents remained the most commonly reported across the study period for all US regions (West, Midwest, South Northeast, and US territories). Conclusions Over a recent 10-year period, common cough preparations remain the most commonly reported intentional abuse ingestion among all years and regions for adolescents.
Acute Mortality and Complications of Cervical Spine Injuries in the Elderly at a Single Tertiary Care Center

Study Design Retrospective database review of all traumatic cervical spine injuries at a single tertiary care center. Objective To determine the acute survival of patients aged 65 and over with a variety of cervical spine injuries, regardless of operative or conservative treatment. Summary of Background Data Elderly patients with cervical spine injuries have historically suffered from high mortality rates. More recent literature has demonstrated improved outcomes among operatively treated elderly, but has suggested that the nonoperative treatment of cervical injuries in this population may itself contribute to increased mortality rates. Methods One thousand seventy-three consecutive patients were identified and initial hospitalization records reviewed. Ninety-four patients were excluded for incomplete data. The remaining 979 patients were divided by age into young and elderly groups. Sex distribution, mechanism, injury type, comorbidities, and mortality and complication rates were compared. Elderly patients were further divided into operative and nonoperative groups and acute outcomes were compared. Results The overall acute mortality rate for all patients with cervical spine injuries was 5.92%. Eighty-six percent of all patients 65 and over survived, as did 96.1% of younger patients. Seventy-three percent of elderly patients with complete injuries survived, as did 80% of those with incomplete injuries, and 95.6% of intact elderly. Acute mortality rates were statistically comparable in both the operatively and nonoperatively treated groups of elderly. Conclusions In this large comprehensive series of elderly patients with cervical spine injuries, statistically comparable survival rates were achieved in both operatively treated and nonoperatively treated patient populations. This finding challenges the conclusion that the nonoperative treatment of the elderly necessarily results in increased acute mortality.
The Governance of Developmental Disability Supports for Older Adults in Ontario and Québec

ABSTRACT The Governance of Developmental Disability Supports for Older Adults in Ontario and Quebec Daniel Dickson This project aims to analyze the effects of Canadian provincial governance structures on support work provision for older adults with developmental disabilities. Drawing from multilevel governance literature, it compares Ontario's more centralized and policy-driven governance structure with Quebec's more disentangled and multi-jurisdictional structure, which gives more autonomy to developmental support agencies in planning support provision. To facilitate this comparison the project uses semi-structured interviews with personal support workers for older adults with developmental disabilities in both provinces. By using an 'institutional ethnography' interview methodology, the work experiences of primary support workers are situated within operant policies and rules, specifically with respect to supporting their clients in 'social inclusion', a widely recognized core domain for quality of life outcomes for adults with developmental disabilities. Owing to dramatic improvements in life expectancy resulting from healthcare advancements and deinstitutionalization, Canadians with developmental disability are increasingly living into older age. Consequently, support work practice is challenged by the intersection of social constructions of aged and disabled identities, which can act against the social inclusion of this ‘new’ population. By comparing these two divergent provincial governance structures from the important perspectives of frontline workers, this project contributes to a discussion of best practices in Canadian policy and administration.
A preliminary study for non-invasive quantification of manganese in human hand bones

Manganese (Mn) is a nutrient essential for regulating neurological and skeletal functions in the human body, but it is also toxic when humans are excessively exposed to Mn. Blood (or serum/plasma) and other body fluids reflect only the most recent exposure and rapidly return to within normal ranges, even when there has been a temporary excursion in response to exposure. In this context, we have been developing a non-invasive measurement of Mn stored in bone, using in vivo neutron activation analysis. Following feasibility studies, a first pilot study, using neutron activation analysis to measure Mn in the bones of the hand of ten healthy male human subjects, was conducted with the approval of the concerned research ethics boards. The participants of this study had no known history of exposure to Mn. Two volunteers were excluded from this study due to technical problems with their measurements. The inverse variance weighted mean value of Mn/Ca for the participants of this study is 0.12 ± 0.68 µg Mn/g Ca which is comparable within uncertainties with the estimated range of 0.16–0.78 µg Mn/g Ca and mean value of 0.63 ± 0.30 µg Mn/g Ca derived from cadaver data. It is recommended to investigate the use of the diagnostic technique for in vivo measurements of workers exposed occupationally to excessive amounts of Mn who could develop many-fold increased levels of Mn in bones as demonstrated through various animal studies. The technique needs further development to improve the precision of in vivo measurements in the non-exposed population.
Sidewinding with minimal slip: Snake and robot ascent of sandy slopes

Limbless organisms such as snakes can navigate nearly all terrain. In particular, desert-dwelling sidewinder rattlesnakes (Crotalus cerastes) operate effectively on inclined granular media (such as sand dunes) that induce failure in field-tested limbless robots through slipping and pitching. Our laboratory experiments reveal that as granular incline angle increases, sidewinder rattlesnakes increase the length of their body in contact with the sand. Implementing this strategy in a physical robot model of the snake enables the device to ascend sandy slopes close to the angle of maximum slope stability. Plate drag experiments demonstrate that granular yield stresses decrease with increasing incline angle. Together, these three approaches demonstrate how sidewinding with contact-length control mitigates failure on granular media. Robots based on sidewinder rattlesnakes are used to understand motion on sloped granular terrain. [Also see Perspective by Socha] What's that coming over the hill—is it a robot? Crossing a slope can be difficult, particularly if it is made of sand. Sidewinder rattlesnakes manage to climb sandy hills by adjusting the length of their body in contact with the sand. Marvi et al. designed robots based on this idea to determine what affects climbing ability on sandy slopes (see the Perspective by Socha). Based on the behavior of the robots, the authors performed further animal studies, and used an iterative approach to improve the robots' capabilities and to better understand animal motion. Science, this issue p. 224; see also p. 160
Thioltransferase in human red blood cells: kinetics and equilibrium.

Thioltransferase from human red blood cells (HRBC TTase), coupled to GSSG reductase, catalyzed glutathione (GSH)-dependent reduction of prototype substrates hydroxyethyl disulfide (HEDS) and sodium S-sulfocysteine as well as of other homo- and heterodisulfides, including the protein mixed disulfide albumin-S-S-cysteine. Whereas apparent KM values for the substrates varied over more than a 20-fold range, the Vmax values agreed quite closely, usually within less than a factor of 2, suggesting that initial interaction of oxidized substrate with enzyme is not rate determining. HRBC TTase was inactivated by iodoacetamide (IAA), and this was prevented by pretreatment with disulfides. The pH dependence of IAA inactivation gave a remarkably low apparent pKa of 3.5, which was independent of ionic strength (0.05-2 M). At pH 6, one radiolabeled carboxyamidomethyl moiety was bound to the enzyme after treatment with [14C]IAA. This unusual thiol reactivity suggests that the active-site cysteine moiety of the TTase may be involved in a hydrogen bond with a carboxylate moiety. In contrast, the pH dependence for GSH-dependent TTase catalysis of disulfide reduction displayed an inflection point near pH 8.0, also suggesting that the initial reaction of oxidized substrate with the active-site thiol is not involved in rate determination. Two substrate kinetic studies of HRBC TTase and rat liver TTase (e.g., [GSH] and [HEDS] varied independently) gave patterns of intersecting lines on double-reciprocal plots (1/v vs 1/S), indicating a sequential mechanism for the TTase reactions, rather than a ping-pong mechanism. (ABSTRACT TRUNCATED AT 250 WORDS)
Mitophagy confers resistance to siderophore-mediated killing by Pseudomonas aeruginosa

Significance Pathogens express virulence factors to support their growth and reproduction while hosts activate various immune processes to promote pathogen clearance and minimize damage. In this study, we establish a new role for pyoverdin, an iron-binding siderophore produced by the bacterium Pseudomonas aeruginosa. In addition to promoting growth by acquiring iron, pyoverdin serves as a secreted bacterial toxin that disrupts mitochondria and iron homeostasis in Caenorhabditis elegans. We show that exposure to pyoverdin triggers mitochondrial damage and subsequent mitophagy (lysosomal degradation of damaged mitochondria). Importantly, mitophagy confers a protective effect against exposure to either pyoverdin or to a synthetic iron chelator, demonstrating a function for mitophagy in innate immunity. Finally, we show that iron chelation causes mitophagy in mammalian cells. In the arms race of bacterial pathogenesis, bacteria produce an array of toxins and virulence factors that disrupt core host processes. Hosts mitigate the ensuing damage by responding with immune countermeasures. The iron-binding siderophore pyoverdin is a key virulence mediator of the human pathogen Pseudomonas aeruginosa, but its pathogenic mechanism has not been established. Here we demonstrate that pyoverdin enters Caenorhabditis elegans and that it is sufficient to mediate host killing. Moreover, we show that iron chelation disrupts mitochondrial homeostasis and triggers mitophagy both in C. elegans and mammalian cells. Finally, we show that mitophagy provides protection both against the extracellular pathogen P. aeruginosa and to treatment with a xenobiotic chelator, phenanthroline, in C. elegans. Although autophagic machinery has been shown to target intracellular bacteria for degradation (a process known as xenophagy), our report establishes a role for authentic mitochondrial autophagy in the innate immune defense against P. aeruginosa.
Study of the Mechanism of TCR Antagonism Using Dual-TCR-Expressing T Cells1

The mechanism of action of TCR antagonists is incompletely understood. T cells expressing two distinct TCRs have been used to test competition for TCR occupancy as a potential mechanism. Previous studies with CD4 T cells showed that an antagonist for one TCR inhibited the response to the other TCR (cross-antagonism), whereas studies with CD8 cells failed to demonstrate cross-antagonism. To determine whether CD4 and CD8 cells were intrinsically different or whether the differences were the result of the use of different effector assays, we studied both CD4 and CD8 dual-TCR-expressing T cells. In the CD4 system, consistent with previous reports, cross-antagonism of proliferation was observed. In the CD8 system, cross-antagonism was observed using proliferation as readout but not when target cell cytolysis was used. These results suggest that different mechanisms may be involved in the inhibition of proliferation and inhibition of cytotoxic effector function, the latter only involving competition for TCR occupancy. Inhibition of proliferation appears to be more complex and other mechanisms such as sequestration of signaling molecules or negative signaling may be involved. The fact that 10- to 20-fold more antagonist was needed to achieve cross-antagonism compared with inhibition of the cognate TCR is consistent with the hypothesis that competition for TCR occupancy is also a major, albeit not sole, mechanism of antagonism of the proliferative responses of CD4 and CD8 cells.
A Randomized Trial on the Efficacy of Prophylactic Active Drainage in Prevention of Complications after Pancreaticoduodenectomy

Background and Aims: This randomized controlled trial was conducted to examine whether prophylactic active drainage decreases morbidity associated with pancreati-coduodenectomy. Material and Methods: A prospective, randomized controlled trial was conducted between April 2010 and May 2015 when 160 consecutive patients following elective pancreaticoduodenectomy were randomized intraoperatively to either prophylactic active drainage group or conventional passive drainage group. The main objectives were the incidence of postoperative pancreatic fistula and the associated clinical outcomes. Results: There were 82 patients in the active drain group and 78 patients in the passive drain group. The overall pancreatic fistula rate occurred similarly in the two groups (11.0% (9/82) vs 14.1% (11/78), p = 0.360). Grade C pancreatic fistula in active drain group was significantly less than that in passive drain group (0% (0/82) vs 6.4% (5/78), p = 0.026). The mean postoperative hospital stay and parenteral nutrition support time in active drainage group were shorter than those in passive drainage group (12.6 days vs 14.5 days, p = 0.037; 6.9 days vs 8.6 days, p = 0.047, respectively). Conclusion: Prophylactic active drainage reveals significant reduction in severity of complications associated with pancreatic fistula and might be recommended as an alternative for patients with high risk of developing serious pancreatic fistula after pancreaticoduodenectomy.
Complement receptor-mediated uptake and tumor necrosis factor-alpha-mediated growth inhibition of Mycobacterium tuberculosis by human alveolar macrophages.

The relative phagocytosis and intracellular fate of Mycobacterium tuberculosis (MTB) (H37Ra) in human alveolar macrophages (AM) and their precursors blood monocytes (MN) was investigated. Uptake of MTB by MN and AM was confirmed by electron microscopy. At an infection ratio of 100:1 (MTB:target cell), the percentage of infected AM and the number of MTB per AM was > MN (p < 0.001, p < 0.0001, respectively). Uptake of MTB was increased by increasing concentrations of serum and decreased in the presence of heat-inactivated serum. Among complement receptors (CR) CR1, CR3, and CR4, the major CR mediating uptake of MTB by MN were CR1 and CR3, whereas for AM, CR4 was the major CR. When MN and AM were infected with MTB and cultured for up to 7 days, AM limited intracellular growth of MTB more effectively than MN as determined by a CFU assay. MTB stimulated production of TNF-alpha by mononuclear phagocytes and by AM > MN (p < 0.007). Pentoxifylline inhibited TNF-alpha production by mononuclear phagocytes and concurrently increased MTB growth (AM > MN). A polyclonal neutralizing antibody to TNF-alpha also increased MTB growth in AM. Thus, AM are more efficient in phagocytosis of MTB than MN, and uptake is mediated through CR4 to a greater extent than CR1 or CR3. The slowed replication of MTB in AM is associated with an increase in TNF-alpha production, and intracellular growth is promoted by pentoxifylline and neutralizing antibody to TNF-alpha. These data suggest that AM may play a prominent and efficient role in the primary defense of the lung in tuberculosis through CR-mediated uptake, predominantly CR4, and TNF-alpha-mediated killing of MTB.
Detection of unknown words and its evaluation

Especially in recognition of spontaneous speech it is necessary to be able to cope with the occurance of unknown words. We present an approach to unknown word detection integrated with speech recognition. Though several schemes exist for the evaluation of detection algorithms all suffer from some deficiencies. Therefore, we also present a new evaluation measure called detection accuracy which is similar to the widely accepted word accuracy. We applied this measure to the evaluation of our approach on a large spontaneous speech recognition task from the German Verbmobil project.
The psychological well‐being and associated factors of caregivers of outpatients with schizophrenia in Taiwan

Abstract The purpose of this study attempted to understand the psychological well‐being of caregivers of individuals with schizophrenia and factors affecting the caregivers’ psychological well‐being. More specifically, quality of life and psychiatric morbidity were used to represent the psychological well‐being. Personal interviews using questionnaires were administrated to 57 caregivers. Data regarding knowledge of schizophrenia, perceived causes of schizophrenia, quality of life and psychiatric morbidity was collected. Psychiatrists assessed the symptoms severity of the schizophrenic patients. Compared with the controls, the psychological well‐being of caregivers of patients with schizophrenia was poorer. There was a significant association between the positive symptoms score and the psychological well‐being of caregivers. Life quality and psychiatric morbidity were affected by caregivers’ knowledge of schizophrenia and beliefs about the cause of schizophrenia. Health care providers should consider the patients and their caregivers as a whole and provide psychoeducaiton to reduce the negative psychological impact of caregivers.
A Pyrosequencing Investigation of Differences in the Feline Subgingival Microbiota in Health, Gingivitis and Mild Periodontitis

Periodontitis is the most frequently diagnosed health problem in cats yet little is known about the bacterial species important for the disease. The objective of this study was to identify bacterial species associated with health, gingivitis or mild periodontitis (<25% attachment loss) in feline plaque. Knowledge of these species is a first step in understanding the potential for improving oral health of cats via dietary interventions that alter the proportions of influential species. Subgingival plaque samples were collected from 92 cats with healthy gingiva, gingivitis or mild periodontitis. Pyrosequencing of the V1-V3 region of the 16S rDNA from these plaque samples generated more than one million reads and identified a total of 267 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all gingival health categories, particularly in health along with Moraxella and Fusobacteria. The Peptostreptococcaceae were the most abundant family in gingivitis and mild periodontitis. Logistic regression analysis identified species from various genera that were significantly associated with health, gingivitis or mild periodontitis. The species identified were very similar to those observed in canine plaque in the corresponding health and disease states. Such similarities were not observed between cat and human at the bacterial species level but with disease progression similarities did emerge at the phylum level. This suggests that interventions targeted at human pathogenic species will not be effective for use in cats but there is more potential for commonalities in interventions for cats and dogs.