Miracle-dz/UniFAR-FTUs huggingface dataset:
Concomitant montelukast and loratadine as treatment for seasonal allergic rhinitis: a randomized, placebo-controlled clinical trial.

BACKGROUND Nasal challenge studies have suggested histamine and cysteinyl leukotrienes are important proinflammatory mediators in allergic rhinitis. This study was designed to determine the efficacy of montelukast, a cysteinyl leukotriene receptor antagonist, administered alone or concomitantly with loratadine, an H(1)-receptor antagonist, in seasonal allergic rhinitis. OBJECTIVE The purpose of this study was to determine the effect of concomitant use of montelukast and loratadine in the treatment of seasonal allergic rhinitis. METHODS In this multicenter (N = 12) double-blind, randomized, parallel-group, placebo-controlled 2-week trial, 460 men and women, aged 15 to 75 years, with spring seasonal allergic rhinitis were randomly allocated to receive 1 of the following 5 treatments: montelukast 10 or 20 mg, loratadine 10 mg, montelukast 10 mg with loratadine 10 mg, or placebo, once daily in the evening. The primary end point was daytime nasal symptoms score (average of congestion, rhinorrhea, itching, and sneezing). Other end points were eye symptoms, nighttime symptoms, individual daytime nasal symptoms, global evaluations (patient's and physician's), and rhinoconjunctivitis quality-of-life scores. RESULTS Concomitant montelukast with loratadine improved the primary end point significantly (P <.001) compared with placebo and each agent alone. Compared with placebo, montelukast with loratadine also significantly improved eye symptoms, nighttime symptoms, individual daytime nasal symptoms, global evaluations, and quality of life. Montelukast alone and loratadine alone caused modest improvements in rhinitis end points. All treatments were similarly well tolerated. CONCLUSIONS Concomitant montelukast with loratadine provided effective treatment for seasonal allergic rhinitis and associated eye symptoms with a safety profile comparable with placebo.
A Comparison Between Unilateral Transverse Process-Pedicle and Bilateral Puncture Techniques in Percutaneous Kyphoplasty

Study Design. A prospective comparative study. Objective. To assess the clinical and radiological outcomes for the treatment of osteoporotic vertebral compression fractures using unilateral transverse process-pedicle and bilateral percutaneous kyphoplasty (PKP). Summary of Background Data. PKP is a widely used vertebral augmentation procedure for treating painful vertebral compression fractures. A percutaneous bilateral approach is typically used to access the vertebral body. Many previous studies have reported excellent clinical results with PKP. In contrast, numerous complications and problems have also been reported, such as puncture difficulty, cement leakage, and adjacent vertebral fracture. Methods. This prospective study included 316 patients with single-level lumbar osteoporotic vertebral compression fracture, 224 females and 92 males with a mean age of 71.5 years. Randomized patients underwent PKP using 2 different puncture techniques. The patients were followed up postoperatively and were assessed mainly with regard to clinical and radiological outcomes. Clinical outcomes were evaluated mainly using the visual analogue scale for pain and 36-Item Short Form Health Survey (SF-36) questionnaire for health status. Radiological outcomes were assessed mainly on the basis of radiation dose, bone cement distribution, vertebral body height, and kyphotic angle. Results. Patients were followed up from 12 to 28 months, with an average of 16.8 months. One hundred fifty-eight patients were treated with unilateral method and 151 patients were treated with bilateral method. In the unilateral group, the volume of the injected cement and radiation dose were significantly less than that in the bilateral group. All patients in both groups had significantly less pain after the procedures, compared with their preoperative period pain. No statistically significant differences were observed when visual analogue scale and 36-Item Short Form Health Survey were compared between the groups. Both unilateral and bilateral groups showed insignificant decrease in the kyphotic angle during the follow-ups. The kyphotic angle in the unilateral group improved more significantly than in the bilateral group. In the bilateral group, 16 patients had obvious pain in the puncture sites at 1 month postoperatively caused by facet joint violation. With local block treatment, the pain disappeared in all patients at the last follow-up. Conclusion. Both bilateral and unilateral PKP are relatively safe and provide effective treatment for patients with painful osteoporotic vertebral compression fracture. However, unilateral PKP received less radiation dose and operation time, it also offered a higher degree of deformity correction and resulted in less complication than bilateral PKP. Level of Evidence: 3
Residents' place image: a cluster analysis and its links to place attachment and support for tourism

ABSTRACT While there is a plethora of studies segmenting the lucrative tourism market, limited attention has been given to identifying potential segments of local residents based on their image of the place they live in as a tourist destination. This study aims to address this gap by (a) clustering local residents of a tourist destination based on their images of that place; and (b) identifying whether those image-based resident groups share similar/different levels of place attachment and intentions toward tourism (support for tourism, intention to recommend it to others). Analysis was based on a sample of 368 residents of Eilat, Israel. The findings suggest the presence of three resident groups with different images of Eilat – called Nature Aesthete, Appreciator, and Critical – and provide support that these groups exhibit dissimilar levels of attachment and intentions/behavior toward tourism. The Appreciator (residents with the most favorable image) were reported exhibiting higher levels of place attachment, support for tourism and were more likely to recommend their place to others as a tourist destination than the Critical (residents with the least favorable image). The implications of these findings to tourism theory and practice are discussed.
Constraints on speciation suggested by comparing lake‐stream stickleback divergence across two continents

Adaptation to ecologically distinct environments can coincide with the emergence of reproductive barriers. The outcome of this process is highly variable and can range along a continuum from weak population differentiation all the way to complete, genome‐wide divergence. The factors determining how far diverging taxa will move along this continuum remain poorly understood but are most profitably investigated in taxa under replicate divergence. Here, we explore determinants of progress towards speciation by comparing phenotypic and molecular divergence within young (<150 years) lake‐stream stickleback pairs from Central Europe to divergence in older (thousands of years) archetypal lake‐stream pairs from Vancouver Island, Canada. We generally find relatively weak divergence in most aspects of foraging morphology (gill raker number, body shape) in the European pairs, although substantial adaptive divergence is seen in gill raker length. Combined with striking overall phenotypic differences between the continents, this argues for genetic and time constraints on adaptive divergence in the European pairs. The European lake‐stream pairs also do not display the strong habitat‐related differentiation in neutral (microsatellite) markers seen in the Canadian watersheds. This indicates either the lack of strong reproductive barriers owing to weak adaptive divergence, or alternatively that neutral markers are poorly suited for detecting reproductive barriers if these emerge rapidly. Overall, our comparative approach suggests constraints on speciation due to genetic architecture and limited time for divergence. The relative importance of these factors remains to be quantified by future investigation.
In vitro fertilization with preimplantation genetic screening.

BACKGROUND Pregnancy rates in women of advanced maternal age undergoing in vitro fertilization (IVF) are disappointingly low. It has been suggested that the use of preimplantation genetic screening of cleavage-stage embryos for aneuploidies may improve the effectiveness of IVF in these women. METHODS We conducted a multicenter, randomized, double-blind, controlled trial comparing three cycles of IVF with and without preimplantation genetic screening in women 35 through 41 years of age. The primary outcome measure was ongoing pregnancy at 12 weeks of gestation. The secondary outcome measures were biochemical pregnancy, clinical pregnancy, miscarriage, and live birth. RESULTS Four hundred eight women (206 assigned to preimplantation genetic screening and 202 assigned to the control group) underwent 836 cycles of IVF (434 cycles with and 402 cycles without preimplantation genetic screening). The ongoing-pregnancy rate was significantly lower in the women assigned to preimplantation genetic screening (52 of 206 women [25%]) than in those not assigned to preimplantation genetic screening (74 of 202 women [37%]; rate ratio, 0.69; 95% confidence interval [CI], 0.51 to 0.93). The women assigned to preimplantation genetic screening also had a significantly lower live-birth rate (49 of 206 women [24%] vs. 71 of 202 women [35%]; rate ratio, 0.68; 95% CI, 0.50 to 0.92). CONCLUSIONS Preimplantation genetic screening did not increase but instead significantly reduced the rates of ongoing pregnancies and live births after IVF in women of advanced maternal age. (Current Controlled Trials number, ISRCTN76355836 [controlled-trials.com].).
E-Service Quality, E-Satisfaction and E-Loyalty of Online Shoppers in Business to Consumer Market; Evidence form Malaysia

The growing usage of internet and online shopping in Malaysia presents a huge prospect in e-commerce market, specifically for B2C segment. As a result, electronic service quality (e-SQ), electronic satisfaction (e-Satisfaction) and electronic loyalty (e-Loyalty) become vital for online retailers to attract and retain online shoppers in this virtual environment. The association between e-SQ, e-Satisfaction and e-Loyalty should be continuously examined to cope with the advancement in information and communication technology, and the changing expectation of online shoppers. However, construct of e-SQ for online retailers in B2C market is still debatable. In this research, E-SERVQUAL was integrated with the other e-SQ scales to measure e-SQ of a prominent online retailer in Malaysia. Specifically, the e-SQ constructs are Efficiency, Privacy and Trust, Fulfilment, Responsiveness, Contact and Website Design. 390 sets of completed and usable questionnaires were gathered using online questionnaire and convenience sampling procedure. The result indicated that the five proposed dimensions of e-SQ constitute e-SQ of online retailer in B2C market. All the dimensions of e-SQ were found to have positive and significant effect on e-Satisfaction of online shoppers. Responsiveness of e-SQ had the strongest impact on e-satisfaction of online shoppers. The shoppers e-Satisfaction was positively and significantly affected their e-Loyalty towards continuous usage of online retailer's website. Managerial and theoretical implications are discussed based on the results of the study.
Factors that affect the flow of patients through triage

Objective: To use observational methods to objectively evaluate the organisation of triage and what issues may affect the effectiveness of the process. Design: A two-phase study comprising observation of 16 h of triage in a London hospital emergency department and interviews with the triage staff to build a qualitative task analysis and study protocol for phase 2; observation and timing in triage for 1870 min including 257 patients and for 16 different members of the triage staff. Results: No significant difference was found between grades of staff for the average triage time or the fraction of time absent from triage. In all, 67% of the time spent absent from triage was due to escorting patients into the department. The average time a patient waited in the reception before triage was 13 min 34 s; the average length of time to triage for a patient was 4 min 17 s. A significant increase in triage time was found when patients were triaged to a specialty, expected by a specialty, or were actively “seen and treated” in triage. Protocols to prioritise patients with potentially serious conditions to the front of the queue had a significantly positive effect on their waiting time. Supplementary tasks and distractions had varying effects on the timely assessment and triage of patients. Conclusions: The human factors method is applicable to the triage process and can identify key factors that affect the throughput at triage. Referring a patient to a specialty at triage affects significantly the triage workload; hence, alternative methods or management should be suggested. The decision to offer active treatment at triage increases the time taken, and should be based on clinical criteria and the workload determined by staffing levels. The proportion of time absent from triage could be markedly improved by support from porters or other non-qualified staff, as well as by proceduralised handovers from triage to the main clinical area. Triage productivity could be improved by all staff by becoming aware of the effect of the number of interruptions on the throughput of patients.
Human immunodeficiency virus type 1 nef quasispecies in pathological tissue

The role of the nef gene in human immunodeficiency virus type 1 (HIV-1) infection is poorly understood. To provide a basis for studies on the role of nef in AIDS, we used targeted polymerase chain reaction amplification and DNA sequencing to determine the structure of nef genes in pathologic tissue from HIV-1-infected children and adults. We find that the nef reading frame is open in 92% of clones derived from both brain and lymphocytic tissue of children, suggesting that nef is expressed in these tissues. One HIV-1 clone, BRVA, obtained by coculture from the brain of an adult AIDS patient with progressive dementia, was previously shown to contain a duplicated region in nef. We show here that similar duplications are widespread in both adults and children with AIDS. However, coculture strongly selects against the broad spectrum of nef quasispecies found in tissue. These findings suggest functional selection for nef quasispecies in pathologic tissues during HIV-1 infection of the human host.
DPPA3 prevents cytosine hydroxymethylation of the maternal pronucleus and is required for normal development in bovine embryos

Dppa3 has been described in mice as an important maternal factor contributed by the oocyte that participates in protecting the maternal genome from oxidation of methylated cytosines (5mC) to hydroxymethylated cytosines (5hmC). Dppa3 is also required for normal mouse preimplantation development. This gene is poorly conserved across mammalian species, with less than 32% of protein sequence shared between mouse, cow and human. RNA-seq analysis of bovine oocytes and preimplantation embryos revealed that DPPA3 transcripts are some of the most highly abundant mRNAs in the oocyte, and their levels gradually decrease toward the time of embryonic genome activation (EGA). Knockdown of DPPA3 by injection of siRNA in germinal vesicle (GV) stage oocytes was used to assess its role in epigenetic remodeling and embryo development. DPPA3 knockdown resulted in increased intensity of 5hmC staining in the maternal pronucleus (PN), demonstrating a role for this factor in the asymmetric remodeling of the maternal and paternal PN in bovine zygotes. Also, DPPA3 knockdown decreased the developmental competence of parthenogenetic and in vitro fertilized embryos. Finally, DPPA3 knockdown embryos that reached the blastocyst stage had significantly fewer ICM cells as compared with control embryos. We conclude that DPPA3 is a maternal factor important for correct epigenetic remodeling and normal embryonic development in cattle, indicating that the role of DPPA3 during early development is conserved between species.
How Familial Is Family Social Capital? Analyzing Bonding Social Capital in Family and Nonfamily Firms

Family social capital (FSC) is acknowledged to be a unique asset that can provide firms with competitive advantages. Certain scholars, however, have questioned whether nonfamily firms can reproduce FSC and benefit from its advantages. If so, FSC may not be as unique as has been assumed. Our study analyzes three types of bonding social capital: capital held by nonfamily firms, and capital held by family and nonfamily members of family firms. We assess the effects that these types of bonding social capital exert on performance. Our empirical analysis of 400 firms suggests that FSC is unique and that nonfamily firms can only attempt to imitate it imperfectly.
Military Expenditure and Economic Growth in SAARC Countries: Pooled and Panel Data Analysis

The purpose of this study is to examine the relationship between military expenditure and economic growth of five SAARC countries by employing the data during the period of 2002 to 2014. Pooled and panel data analyses have been applied on the data. In addition, Pooled Ordinary Least Square Model is used to analyze the regression results. The empirical results show that there is significant and positive relationship between military expenditure and economic growth. This study contributes in the literature and the results of this study have implications for military and economic policymaking.
The Internet Memes

The increasing role of the internet in everyday life has affected the process of transmission of cultural ideas – today cultural phenomenons known as memes can be transferred across the borders of societies, states and cultures faster than ever, rapidly reaching the peak of popularity, self-replicating and mutating not unlike the genes of a living organism. Learn more about the concept of a meme, the science that studies these units of culture, as well as some of the most popular internet phenomena in this book.
BREEDING AND GENETICS Development of an Improved Method of Mitotic Metaphase Chromosome Preparation Compatible for Fluorescence in situ Hybridization in Cotton

Molecular cytogenetic techniques, especially Fluorescence In situ Hybridization (FISH) and Genomic In situ Hybridization (GISH), are excellent tools to study the structure and function of chromosomes, polyploidy, aneuploidy, alien gene introgression and genome evolution and physical mapping of genes. For many applications, in situ hybridizations require reliable and efficient methods of chromosome preparation with well preserved and dispersed chromosomes and little or no cell wall debris. However, such protocols have not yet been published for cotton mitotic chromosome preparations. In the current study, an improved method for preparing mitotic metaphase chromosomes of tetraploid cotton was developed. Root tips were collected from lab-grown three-day-old cotton seedlings and pre-treated with high-pressure nitrous oxide (N2O). Following fixation with acetic acid, root tip meristems were removed, enzymatically digested, gently macerated, and then processed to create suspensions of protoplasts in acetic acid and methanol. Suspended protoplasts were then dropped onto a glass slide in order to distribute the protoplasts and spread the chromosomes. Using this approach, acceptable mitotic indexes and high-quality chromosomal spreads with no cell wall debris were obtained. This drop method of preparing chromosome spreads was tested for its compatibility for in situ hybridization using rDNA as probe in FISH. Additionally, varying pre-treatment times with N2O were investigated. Our results show that the combination of a 95-minute N2O pre-treatment, a 55-minute enzymatic degradation, and the “drop method” efficiently produce cotton mitotic metaphase preparations. This new method will expectedly improve the efficiency and efficacy of GISH, FISH, and single-copy multicolor FISH, which will have a multitude of ramifications in cotton genetics and breeding.
Validation of stress indicators for the assessment of animal welfare and prediction of pork meat quality variation at commercial level

The objectives of this study were to assess the relationship between blood lactate variation measured at the plant, and pork quality variation on a large sample size and under commercial preslaughter handling conditions. A total of 600 pigs were randomly chosen on arrival at a commercial slaughter plant and blood samples taken from the ear vein at unloading (UN), after lairage (LA), in the restrainer (RE; before stunning), and at exsanguination (EX) were analysed for lactate content using a Lactate Scout Analyzer (LSA). In order to have a large range of measures, pigs were distributed into two groups; one kept in lairage overnight (G1) and the other for 2-3 h (G2) before slaughter. Meat quality was assessed in the Longissimus thoracis (LT), Semimembranosus (SM) and Adductor (AD) muscles by measuring the pH 30 min post-mortem (pH1) and at 24 h post-mortem (pHu), the colour and the drip loss. Blood lactate levels did not differ between G1 and G2 (P > 0.05). A reduced muscle lactate
Photosynthetic microbial fuel cells with positive light response

The current study introduces an aerobic single‐chamber photosynthetic microbial fuel cell (PMFC). Evaluation of PMFC performance using naturally growing fresh‐water photosynthetic biofilm revealed a weak positive light response, that is, an increase in cell voltage upon illumination. When the PMFC anodes were coated with electrically conductive polymers, the rate of voltage increased and the amplitude of the light response improved significantly. The rapid immediate positive response to light was consistent with a mechanism postulating that the photosynthetic electron‐transfer chain is the source of the electrons harvested on the anode surface. This mechanism is fundamentally different from the one exploited in previously designed anaerobic microbial fuel cells (MFCs), sediment MFCs, or anaerobic PMFCs, where the electrons are derived from the respiratory electron‐transfer chain. The power densities produced in PMFCs were substantially lower than those that are currently reported for conventional MFC (0.95 mW/m2 for polyaniline‐coated and 1.3 mW/m2 for polypyrrole‐coated anodes). However, the PMFC did not depend on an organic substrate as an energy source and was powered only by light energy. Its operation was CO2‐neutral and did not require buffers or exogenous electron transfer shuttles. Biotechnol. Bioeng. 2009; 104: 939–946. © 2009 Wiley Periodicals, Inc.