Miracle-dz/UniFAR-FTUs huggingface dataset:
Growth patterns and body composition of transgenic mice expressing mutated bovine somatotropin genes.

The objectives of this study were to determine growth rates, feed intakes, feed efficiencies, and chemical composition of mice from three transgenic lines in 10-d periods from weaning to near maturity. Lines M4, M11, and G119K express bovine somatotropin (bST) mutations E117L, L121P-E126G, and G119K and display phenotypes of large, near normal, and small body size, respectively. M4 mice were 28% larger at 28 d and 84% larger at 68 d than non-transgenic control (NTC) mice. M11 mice were the same size at 28 d as NTC but were 25% larger at 68 d. G119K mice were 34% and 25% smaller than NTC at 28 and 68 d, respectively. Growth rates of G119K mice and NTC were similar, whereas growth rates of M11 and M4 mice were increased (P < .05). Feed intakes of M4 and M11 mice were greater than those of NTC mice (P < .05), whereas feed intakes of G119K mice were lower than those of NTC mice (P < .05). Feed efficiency (gain/feed) was improved in M4 and M11 mice (P < .05) and not altered in G119K mice compared to that of NTC mice (P > .05). Chemical composition was also altered by expression of bST analogs in transgenic mice. G119K and M4 mice had increased body fat percentages and decreased body protein percentages in comparison to M11 and NTC mice (P < .05). (ABSTRACT TRUNCATED AT 250 WORDS)
Image-guided Craniotomy for Cerebral Metastases: Techniques and Outcomes

OBJECTIVEThe purpose of the present study was to analyze the outcomes after craniotomies for brain metastases in a modern series using image-guided technologies either in the regular operating room or in the intraoperative magnetic resonance imaging unit. METHODSNeurosurgical outcomes were analyzed for 49 patients who underwent 55 image-guided craniotomies for excision of brain metastases during a 5-year period. Tumors were located in critical and noncritical function regions of the brain. A total of 23 craniotomies for tumors in critical brain were performed using intravenous sedation anesthesia; craniotomies for noncritical function brain regions were completed under general anesthesia. The patients were also divided into Radiation Therapy Oncology Group recursive partitioning analysis (RPA) classes on the basis of age, Karnofsky Performance Scale scores, state of primary disease, and presence or absence of extracranial metastases. RESULTSThere was no perioperative mortality. Gross total resection, as verified by postoperative contrast-enhanced computed tomography or magnetic resonance imaging, was achieved in 96% of patients. The median anesthesia time was 4.25 hours, and the median length of hospital stay was 3 days. In 51 symptomatic cases, there was complete resolution of symptoms in 70% (n = 36), improvement in 14% (n = 7), and no change in 12% (n = 6) postoperatively. No patient who was neurologically intact preoperatively deteriorated after surgery, and 93% of patients maintained or improved their functional status. Only two patients (3.6%) with significant preoperative deficits had increased long-term deficits postoperatively. The mean follow-up was 1 year, and the local recurrence rate was 16%. The median survival of the entire group was 16.23 months (17.5 mo in RPA Class I, 22.9 mo in RPA Class II, and 9.8 mo in RPA Class III). CONCLUSIONGross total resection of brain metastases, including those involving critical function areas, can be safely achieved with a low morbidity rate using contemporary image-guided systems. RPA Class I and II patients with controlled primary disease benefit from aggressive treatment by surgery and radiation.
A 60-GHz Millimeter-wave CMOS Marchand Balun

A novel 60-GHz wideband Marchand balun fabricated with a standard 0.18 mum 6-metal-layer CMOS process for millimeter-wave applications is presented. A technique for achieving good balance with the fourth metal layer microstrip conductor is used in the designed Marchand balun. The fabricated CMOS balun uses multilayer coupling with the top two layers. An output matching network is added to improve the output return loss. The measured amplitude imbalance was about plusmn 1.5 dB from 25 to 65 GHz , over the 89% operating frequency band.
Update on toll-like receptor-directed therapies for human disease

Innate responses to microbes are mediated in large part by toll-like receptors (TLRs), which recognise a diverse range of molecules produced by viruses, bacteria and fungi. Great effort has been directed towards translating this knowledge into the development of new therapies for a wide spectrum of diseases, including infectious, malignant, autoimmune and allergic diseases. This review will provide a brief update on completed, ongoing and planned clinical trials of TLR ligand-based therapies for the treatment of diseases in humans.
Existence of Distinct GB Virus C/Hepatitis G Virus Variants with Different Tropism

ABSTRACT To study the existence of GB virus C/hepatitis G virus (GBV-C/HGV) variants with different tropism, we have analyzed the heterogeneity and quasispecies composition of GBV-C/HGV isolated from in vitro-infected peripheral blood mononuclear cells (PBMC) and from sera, livers, and PBMC from two chronically infected patients. For this purpose, the GBV-C/HGV 5′ noncoding region (5′NCR) was amplified by reverse transcription-PCR and the amplified products were cloned and sequenced. These analyses showed that the master 5′NCR sequences isolated from the in vitro-infected PBMC and from the PBMC isolated from the patient whose serum was used as the inoculum were identical but different from that of the inoculum. Furthermore, phylogenetic analysis revealed that all PBMC sequences grouped together into a branch which was separate from those of the inoculum. For one of the two chronically infected patients, all the sequences from the PBMC and one from the liver clustered into a single branch while the sequences from the serum and all the other liver sequences grouped together in the other branch. For the other patient, the sequences from the serum and PBMC and three sequences from the liver grouped together into one branch, while the remaining five sequences from the liver were separated in a different cluster. In conclusion, our results support the existence of different GBV-C/HGV variants with different tissue tropism.
The Labor Market Channel of Macroeconomic Uncertainty

Uncertainty has recently become a major concern for policymakers and academics. Spikes in uncertainty are often associated with recessions and have detrimental effects on the aggregate economy. This paper analyzes the effects of uncertainty on firms' hiring and investment decisions, both empirically and theoretically. Empirically, VAR estimates show the negative effects of uncertainty on economic performance and in particular on the labor market. Counterfactual experiments highlight the significant role of hiring decisions as a transmission channel for uncertainty. The empirical findings are rationalized through a DSGE model with search and matching frictions in the labor market and stochastic volatility. The model is able to replicate the observed co-movement among consumption, investment, output and labor market outcomes generated by an uncertainty shock. Price stickiness greatly amplifies the reaction of the economy. Simulations show that monetary policy can mitigate the adverse effects of uncertainty by adopting a strong anti-inflationary policy.
Chronic cocaine disrupts neurovascular networks and cerebral function: optical imaging studies in rodents

Abstract. Cocaine abuse can lead to cerebral strokes and hemorrhages secondary to cocaine’s cerebrovascular effects, which are poorly understood. We assessed cocaine’s effects on cerebrovascular anatomy and function in the somatosensory cortex of the rat’s brain. Optical coherence tomography was used for in vivo imaging of three-dimensional cerebral blood flow (CBF) networks and to quantify CBF velocities (CBFv), and multiwavelength laser-speckle-imaging was used to simultaneously measure changes in CBFv, oxygenated (Δ[HbO2]) and deoxygenated hemoglobin (Δ[HbR]) concentrations prior to and after an acute cocaine challenge in chronically cocaine exposed rats. Immunofluorescence techniques on brain slices were used to quantify microvasculature density and levels of vascular endothelial growth factor (VEGF). After chronic cocaine (2 and 4 weeks), CBFv in small vessels decreased, whereas vasculature density and VEGF levels increased. Acute cocaine further reduced CBFv and decreased Δ[HbO2] and this decline was larger and longer lasting in 4 weeks than 2 weeks cocaine-exposed rats, which indicates that risk for ischemia is heightened during intoxication and that it increases with chronic exposures. These results provide evidence of cocaine-induced angiogenesis in cortex. The CBF reduction after chronic cocaine exposure, despite the increases in vessel density, indicate that angiogenesis was insufficient to compensate for cocaine-induced disruption of cerebrovascular function.
Dietary patterns and their association with sociodemographic factors in Lithuanian adult population.

SUMMARY The aim of the study was to determine the association between dietary patterns and sociodemographic factors using the data of Lithuanian health behavior monitoring. MATERIAL AND METHODS In 1998-2004, four cross-sectional surveys were carried out within the Finbalt Health Monitor project. For every survey, a random sample of 3000 Lithuanians aged 20-64 years was taken from the National Population Register by mailed questionnaires. The response rates varied from 61.7% to 74.4%. Factor analysis was employed in order to reduce the number of food items. Four main factors were defined: "light food," "sweets," "heavy food," and "cereals" factors. The associations between dietary patterns and sociodemographic factors were examined by applying a logistic regression analysis. RESULTS Four major factors accounted for 45.9% of the total variance in food intake in men and 46.5% in women. The "sweets" factor was inversely associated with age both in men and women. A strong positive association was found between age and the "cereals" factor. Men and women with a higher level of education were more likely to follow "light food" and "sweets" pattern and less likely "heavy food" if compared with people with a lower level of education. The odds of consuming "light food" were higher in cities than in rural areas. Married men and women were more likely to follow "heavy food" pattern compared to unmarried. CONCLUSION This study identified four main dietary patterns in Lithuanian population. Dietary patterns of people with a higher level of education and inhabitants of cities are closer to the recommendations on healthy nutrition.
[The expression of cytokines in experimental otitis media with effusion].

OBJECTIVE To explore the relationship between type I allergic reaction and OME and the expression of Th1, Th2 cytokines in the middle ear mucosa in OME. METHOD BALB/C mice were randomly divided into experimental group (Exp), control group I (Con1) and control group II (Con2), BALB/C mice were sensitized to ovalbumin (OVA) and challenged transtympanically. The thickness of mucosa, the infiltration of eosinophils were detected by hematotoxylin-eosin staining (HE staining), the expression of IL-4, IL-5, IFN-gamma and IL-12 were examined by immunohistochemistry. RESULT In experimental group, the expression of IL-4, IL-5, the thicknesss of mucosa and the numbers of eosinophils were significantly higher than those of Con1, Con2. But the expression of IFN-gamma, IL-12 were both significantly low. The concentration of IL-5 were positively correlated with the number of eosinophils in mucosa. CONCLUSION This study demonstrates that type I allergic reaction, Th2 cytokines have an important effect on OME.
A Semimechanistic Pharmacokinetic-Enzyme Turnover Model for Rifampin Autoinduction in Adult Tuberculosis Patients

ABSTRACT The currently recommended doses of rifampin are believed to be at the lower end of the dose-response curve. Rifampin induces its own metabolism, although the effect of dose on the extent of autoinduction is not known. This study aimed to investigate rifampin autoinduction using a semimechanistic pharmacokinetic-enzyme turnover model. Four different structural basic models were explored to assess whether different scaling methods affected the final covariate selection procedure. Covariates were selected by using a linearized approach. The final model included the allometric scaling of oral clearance and apparent volume of distribution. Although HIV infection was associated with a 30% increase in the apparent volume of distribution, simulations demonstrated that the effect of HIV on rifampin exposure was slight. Model-based simulations showed close-to-maximum induction achieved after 450-mg daily dosing, since negligible increases in oral clearance were observed following the 600-mg/day regimen. Thus, dosing above 600 mg/day is unlikely to result in higher magnitudes of autoinduction. In a typical 55-kg male without HIV infection, the oral clearance, which was 7.76 liters · h−1 at the first dose, increased 1.82- and 1.85-fold at steady state after daily dosing with 450 and 600 mg, respectively. Corresponding reductions of 41 and 42%, respectively, in the area under the concentration-versus-time curve from 0 to 24 h were estimated. The turnover of the inducible process was estimated to have a half-life of approximately 8 days in a typical patient. Assuming 5 half-lives to steady state, this corresponds to a duration of approximately 40 days to reach the induced state for rifampin autoinduction.
Determining the prevalence of Oesophagostomum bifurcum and Necator americanus infections using specific PCR amplification of DNA from faecal samples

Until recently infection of humans with Oesophagostomum bifurcum was regarded as a rare zoonosis. But in northern Togo and Ghana its prevalence is 50% or more in certain villages. Diagnosis is hampered by the fact that the eggs of O. bifurcum are morphologically identical to those of the hookworm Necator americanus. Stools have to be cultured for 7 days to allow eggs to hatch to the characteristic third‐stage (L3) larvae. We evaluated the applicability of specific polymerase chain reactions (PCRs) to amplify DNA from faecal samples as an alternative method for the differential diagnosis of the two infections. Oesophagostomum bifurcum‐PCR was positive in 57 of 61 faecal samples known to contain O. bifurcum L3 larvae in coproculture. Necator americanus PCR was positive in 137 of 146 faecal samples known to contain N. americanus L3 larvae in coproculture. PCR also detected 26 additional O. bifurcum cases in 72 samples from O. bifurcum endemic villages in which no O. bifurcum larvae were found and 45 N. americanus cases in 78 samples in which no N. americanus larvae were found in coproculture. No O. bifurcum DNA was detected in 91 stool samples from individuals from two non‐endemic villages. These results prove the usefulness of specific PCR assays as epidemiological tools to estimate the prevalence of O. bifurcum and N. americanus infections in human populations.
Multiple sclerosis with and without CSF bands: Clinically indistinguishable but immunogenetically distinct

We sought to determine whether Swedish patients with multiple sclerosis (MS) with and without oligoclonal bands (OCBs) in the CSF constitute distinct subpopulations, clinically and immunogenetically. Our findings indicate that OCB-negative MS shares the same clinical features as OCB-positive MS regarding female predominance, age at onset, proportion of primary progressive cases, rate of MRI positivity, and disease severity. Our HLA-DRB1 genotyping results suggest, however, that OCB-positive and OCB-negative MS are immunogenetically distinct.
Knee bracing after ACL reconstruction: effects on postural control and proprioception.

PURPOSE To evaluate the effects an anterior cruciate ligament (ACL) brace has on various measures of knee proprioception and postural control. METHODS Thirty subjects (mean age 27 +/- 11 yr) having undergone unilateral ACL reconstruction were tested with and without wearing their own custom-fit brace on their involved limb. Proprioception was assessed using joint angle replication tests completed on an isokinetic dynamometer. Postural control was assessed using a series of single-limb standing balance tests completed on a force platform. The balance tests included: 1) standing on the stable platform with eyes open, 2) standing on a foam mat placed over the platform with eyes open, 3) standing on the platform with eyes closed, and 4) standing on the platform after landing from a maximal single-limb forward hop. RESULTS The brace provided a small but statistically significant improvement in proprioception (mean reduction in error scores between target and reproduced angles = 0.64 +/- 1.4 degrees, P = 0.02). For the postural control tests, there was a significant brace condition by test situation interaction (P = 0.02), with the brace providing a small but statistically significant improvement during the test completed on the stable platform with eyes open (mean reduction in center of pressure path length = 4.2 +/- 8.4 cm, P = 0.02) but not during the other more challenging test situations. Additional post hoc analyses indicated that the relationship between knee proprioception and postural control measures were low and not significant (r = 0.003 to 0.19, P > 0.32), consistent with the suggestion that changes in knee proprioception can occur in the absence of substantial changes in postural control. Also, standing balance tests that challenged the somatosensory contribution to postural control (i.e., those completed on foam, or with eyes closed) were significantly related to single-limb forward hop distances (r = -0.4, P < 0.05), whereas performance during the proprioception test was not (r = 0.1, P > 0.50). CONCLUSIONS In general, bracing appears to improve performance during tasks characterized by relatively limited somatosensory input but not during tasks characterized by increased somatosenory input. The small magnitude of the improvements, coupled with their apparent lack of carry over to more difficult and functionally relevant tasks, questions the clinical benefit of the present effects of bracing.
Effects of cell swelling on intracellular calcium and membrane currents in bovine articular chondrocytes

Chondrocytes experience a dynamic extracellular osmotic environment during normal joint loading when fluid is forced from the matrix, increasing the local proteoglycan concentration and therefore the ionic strength and osmolarity. To exist in such a challenging environment, chondrocytes must possess mechanisms by which cell volume can be regulated. In this study, we investigated the ability of bovine articular chondrocytes (BAC) to regulate cell volume during a hypo‐osmotic challenge. We also examined the effect of hypo‐osmotic stress on early signaling events including [Ca2+]i and membrane currents. Changes in cell volume were measured by monitoring the fluorescence of calcein‐loaded cells. [Ca2+]i was quantified using fura‐2, and membrane currents were recorded using patch clamp. BAC exhibited regulated volume decrease (RVD) when exposed to hypo‐osmotic saline which was inhibited by Gd3+. Swelling stimulated [Ca2+]i transients in BAC which were dependent on swelling magnitude. Gd3+, zero [Ca2+]o, and thapsigargin all attenuated the [Ca2+]i response, suggesting roles for Ca2+ influx through stretch activated channels, and Ca2+ release from intracellular stores. Inward and outward membrane currents significantly increased during cell swelling and were inhibited by Gd3+. These results indicate that RVD in BAC may involve [Ca2+]i and ion channel activation, both of which play pivotal roles in RVD in other cell types. These signaling pathways are also similar to those activated in chondrocytes subjected to other biophysical signals. It is possible, then, that these signaling events may also be involved in a mechanism by which mechanical loads are transduced into appropriate cellular responses by chondrocytes. J. Cell. Biochem. 86: 290–301, 2002. © 2002 Wiley‐Liss, Inc.
A prospective study of weight gain associated with chronotype among college freshmen

A prospective study of chronotype as a predictor of increased weight gain and body mass index (BMI) among college freshman was undertaken. At baseline, 137 college freshmen were characterized as morning, neutral, or evening types using the reduced version of the Morningness-Eveningness Questionnaire. Additionally, information was collected regarding weight, BMI, and health habits (e.g., junk food and alcohol consumption). These additional measures consisted of a descriptive questionnaire, the Pittsburgh Sleep Quality Index, the International Physical Activity Questionnaire, the Gray-Donald Eating Patterns Questionnaire, and the Positive and Negative Affect Scale. Participants included 79 females and 80 males with a mean age of 18.25 (SD = 0.56) yrs. Eight weeks later, participants returned (N = 54) to complete follow-up measures, which were identical to baseline assessments with the exception of the descriptive questionnaire, in which demographic questions were removed. Evening types had a significantly greater BMI gain (p < 0.05) when compared with morning/neutral types. Health behaviors did not differ by chronotype. Future studies should seek to clarify the mechanisms underlying the chronotype-BMI/weight gain relationship. (Author correspondence: jdk29@drexel.edu)
Sustained linear growth and preserved renal function in 10‐year survivors of pediatric liver transplantation

The aim of this study was to characterize the clinical outcomes of children and adolescents who achieved survival of more than 10 years following liver transplantation (LT) in a single center in Korea. From June 1996 to October 2003, 57 pediatric LTs were performed. The medical records of 44 patients who had survived more than 10 years were reviewed retrospectively. Median age of patients at LT was 0.8 years. Forty‐one patients received living donor LT, and three patients received deceased donor LT. Biliary atresia was the most common indication (65.9%). Thirty‐five patients were on tacrolimus monotherapy at 10 years post‐LT with a mean trough level of 2.73 ng/ml, and five patients were maintaining stable graft function without any immunosuppression. There were no patients receiving antihypertensive medication and one case of diabetes mellitus. Renal dysfunction was seen in two patients (4.5%), while none required renal replacement therapy. Mean height z‐score prior to LT was −1.35 and at 10 years post‐transplant was 0.05. Good linear growth was sustained in this cohort throughout the 10 years, approaching the 50th percentile. Also, there were remarkably low incidences of renal dysfunction and patients requiring medications for glycemic or hypertensive control, all hallmarks of continued use of immunosuppressive agents.