Miracle-dz/UniFAR-FTUs huggingface dataset:
Rolipram, a Selective Phosphodiesterase Type‐IV Inhibitor, Prevents Induction of Heat Shock Protein HSP‐70 and hsp‐70 mRNA in Rat Retrosplenial Cortex by the NMDA Receptor Antagonist DizociIpine

The non‐competitive NMDA receptor antagonists, such as (+)‐MK‐801 (dizocilpine), cause the expression of heat shock protein HSP‐70 and pathomorphological damage in the retrosplenial cortex of the rat brain. However, the precise mechanism(s) underlying the neurotoxicity of NMDA receptor antagonists is unknown. The present study was undertaken to examine the role of phosphodiesterase type IV in the expression of heat shock genes induced by dizocilpine. Heat shock protein HSP‐70, which is known as a sensitive marker of neuron injury, was induced in the retrosplenial cortex of the rat brain 24 h after a single administration of dizocilpine (1 mg/kg). Pretreatment with the specific phosphodiesterase type IV inhibitor rolipram (2.5, 5 or 10 mg/kg, 15 rnin before dizocilpine) attenuated the expression of HSP‐70 and hsp‐70 mRNA induced by dizocilpine (1 mg/kg) in a dose‐dependent manner. Furthermore, another phosphodiesterase type IV inhibitor, Ro 20–1724 (5 or 10 mg/kg, 15 min before dizocilpine), and a non‐selective phosphodiesterase inhibitor, 3–isobutyl‐1–methylxanthine (IBMX) (5 or 10 mg/kg, 15 min before dizocilpine), significantly attenuated the expression of HSP‐70 protein and hsp‐70 mRNA induced in the retrosplenial cortex by dizocilpine. However, the induction of the immediate early gene c‐fos and microglial activation in the retrosplenial cortex after administration of dizocilpine was not attenuated by pretreatment with rolipram (5 or 10 mg/kg, 15 min before dizocilpine). Moreover, histopathological study indicated that pretreatment with rolipram (5 or 10 mg/kg, 15 min before dizocilpine) did not prevent the formation of vacuoles caused by treatment with dizocilpine. The present findings suggest that phosphodiesterase type IV may play a significant role in the expression of HSP‐70 protein and hsp‐70 mRNA in the rat retrosplenial cortex after administration of dizocilpine, and that phosphodiesterase type IV may not play a role in the neurotoxicity of NMDA receptor antagonists such as dizocilpine.
Detection of Giardia lamblia andCryptosporidium parvum Antigens in Human Fecal Specimens Using the ColorPAC Combination Rapid Solid-Phase Qualitative Immunochromatographic Assay

ABSTRACT The ColorPAC Giardia/Cryptosporidium (Becton Dickinson) is a solid-phase qualitative immunochromatographic assay that detects and distinguishes between Giardia lamblia andCryptosporidium parvum in human stool. Agreement between the Alexon-Trend ProSpecT Giardia Rapid EIA and the ColorPAC assay was 166 of 172 (96.5%). Agreement between the Alexon-Trend ProSpecT Cryptosporidium Rapid EIA and the ColorPAC assay was 169 of 171 (98.8%). No cross-reactions were seen with other parasites or human cells.
Vectorial approach to coupled nonlinear Schrödinger systems under nonlocal Cauchy conditions

The paper presents a vectorial approach for coupled general nonlinear Schrödinger systems with nonlocal Cauchy conditions. Based on fixed-point principles, the use of matrices with spectral radius less than one, and on basic properties of the Schrödinger solution operator, several existence results are obtained. The essential role of the support of the nonlocal Cauchy condition is emphasized and fully exploited.
Electronic band structure of the superconductor Sr2RuO4.

A local-density electronic-band-structure calculation was performed for a recently discovered non-copper-layered perovskite superconductor, Sr[sub 2]RuO[sub 4]. It was found that the electronic structure near the Fermi energy is essentially described by antibonding bands of the Ru [ital d][var epsilon] and O [ital p][pi] states. Although two holes in the bands are predominantly situated in a [ital d][var epsilon]([ital xy])-[ital p][pi] state in the [ital ab] plane, the hole occupations in the other [ital d][var epsilon]-[ital p][pi] states vertical to the plane are not negligibly small, possibly in conjunction with the smallness of tetragonal distortion of the RuO[sub 6] octahedron. Associated with the antibonding [ital d][var epsilon]-[ital p][pi] bands, the density of states at the Fermi energy is relatively high (4.36 states/eVcell) but not enough to account for the observed specific-heat constant [gamma][sub exp] and temperature-independent magnetic susceptibility [chi][sub exp]. We found a large Stoner factor, which may explain most of the mass enhancement involved in [chi][sub exp]. Certain similarities and dissimilarities in the electronic properties to the cuprate superconductors are discussed.
Evaluation of the accuracy of serum human epididymis protein 4 in combination with CA125 for detecting ovarian cancer: a prospective case-control study in a Korean population

Abstract Background: This study aimed to determine the serum concentrations of CA125 and human epididymis protein 4 (HE4) in patients with ovarian cancer, and to evaluate the sensitivity and specificity of these biomarkers for differentiating between patients with benign gynecological disease and those with ovarian cancer, when used alone and in combination in a Korean population. Methods: We consecutively recruited 159 women with an adnexal mass, including 78 women with ovarian cancer. A total of 224 healthy women served as controls. The serum concentrations of HE4 and CA125 were analyzed using immunochemiluminescence assays. The concentrations of the markers were compared among the different subgroups, and the diagnostic accuracy of each marker and the combination of the two markers was assessed by plotting receiver operating characteristic (ROC) curves. In addition, the Risk of Ovarian Malignancy Algorithm (ROMA) was utilized to categorize patients into low- and high-risk groups for epithelial ovarian cancer. Results: Serum HE4 and CA125 concentrations were significantly higher in the ovarian cancer patients compared with those seen in patients with benign disease or in the healthy controls (p<0.0001 in both). In patients with an adnexal mass, the area under the ROC curve was higher when the combination of the markers was used compared with use of CA125 only. Using ROMA, patients could be successfully classified into high- and low-risk group, with 87.5% sensitivity at a specificity of 93.8%. Conclusions: These findings suggest that measuring serum HE4 concentrations along with CA125 concentrations may provide higher accuracy for detecting ovarian cancer.
FATORES QUE INFLUENCIAM AS COMPETÊNCIAS EM DOCENTES DE CIÊNCIAS CONTÁBEIS

O tema competencia tem estado presente nas discussoes academicas e empresariais, nos ultimos anos, associado a diferentes instâncias de compreensao: no nivel da pessoa, das organizacoes e dos paises. Diante desse contexto, o presente estudo teve como objetivo verificar quais os fatores que influenciam no desenvolvimento de competencias nos professores de Ciencias Contabeis. Foi utilizada a pesquisa de campo, com aplicacao de questionario junto aos docentes dos cursos de Ciencias Contabeis localizados nas capitais de todos os Estados do Nordeste, obtendo-se uma amostra de 267 respondentes. Os resultados apontam que os fatores propostos apresentam diferenca significativa nas competencias utilizadas na pesquisa. Palavras-chave: Competencias. Docentes. Ciencias Contabeis.
Study of induced strain in silicon rib structures

This paper reports a theoretical and experimental study on induced strain in silicon-based rib structures. Simulations of induced stress and strain distribution were performed for nitride-strained silicon; moreover, locally-accurate strain measurements were performed on manufactured rib structures in proximity of the nitride-to-silicon interface employing the Convergent Beam Electron Diffraction (CBED) technique. The study resulted in good accordance between simulated and measured strain behaviors along the rib cross-section, indicating the possibility to achieve significant strain levels (e.g. higher than 2 mε for the εzz strain tensor component).
International Liquidity Rents

This paper presents a model of global liquidity shortages. Liquid claims are enforceable promises that play a transaction role. Since developed economies have a comparative advantage in creating liquidity, they export liquid claims to emerging economies, resulting in a permanent current account deficit. This model suggests that unrestricted liquidity flows are (a) welfare reducing for emerging economies and (b) Pareto inefficient. The inefficiency results both from excessive investment for the purpose of creating collateral-backed liquid claims, and from excessive global fragility with respect to collateral shocks.
Comparison of the characteristics of human gingival fibroblasts isolated by tissue explants and by enzyme digestion methods

Objective: To compare the characteristics of human gingival fibroblasts (hGFs) obtained by tissue-explant and enzyme-digestion methods. Methods: The hGFs were isolated using tissue explants and enzyme digestion methods and identified by immunocytochemical analysis. Cell-surface molecules were detected by flow cytometry analysis. The colony-forming activity was examined by CFU-F assay. Oil red O staining and alizarin red staining were performed to identify the adipogenic and osteogenic differentiation of hGFs and the differentiation capacity was quantified using spectrophotometric method. Results: Flow cytometry analysis showed that hGFs expressed CD44, CD29, Strol-1, but not CD34 and CD45. Strol-1 was significantly higher in enzyme-digestion hGFs [(14.84±2.00)%] than in tissue-explant hGFs [(6.64±0.95)%] (P<0.05). CFU-F assay showed that the self-renewal capacity of hGFs in enzyme-digestion group [(21±8)%] was significantly higher than that in tissue-explant group [(14±8)%] (P<0.05). The quantification test of adipogenic differentiation capacity showed the absorbance of enzyme-digestion group [0.66±0.03] was much higher than that tissue-explant group [0.45±0.04] (P<0.05). And the similar result was showed in the osteogenic differentiation capacity test that the absorbance of enzyme-digestion group [0.14±0.01] was obviously higher than that tissue-explant group [0.12±0.01] (P<0.05). Conclusions: Compared with tissueexplant hGFs, enzyme-digestion hGFsshowed much more mesenchymal stem cell-like properties with significantly higher self-renewal capacity and adipogenic and osteogenic differentiation ability.
A comparison of acute anterior cruciate ligament examinations

The preoperative examination, the examination under anesthesia, and the findings at surgery were compared for acutely injured knees that were found at surgery to have anterior cruciate ligament (ACL) tears. The pivot shift was initially positive in only 35% of the knees; however, under anesthesia 98% were pos itive. The Lachman test was initially positive in 99% of the knees, and under anesthesia, 100%. The anterior drawer sign was present initially in 70% of the knees, and under anesthesia this increased to 91 %. Of the 37 patients with isolated ACL tears, only 20 (54%) had initially positive anterior drawer signs. In the knees in which secondary restraints to anterior-poste rior motion were injured, this percentage increased to 69% in medial meniscus injury, 82% in lateral meniscus injury and 89.5% in rupture of the medial collateral ligament (MCL). The Lachman test seems relatively unaffected by associated ligamentous or meniscal in juries, but the end point in the Lachman test is absent in all complete ACL tears. The pivot shift phenomenon, which was demonstrable in 98% of patients when tested under anesthesia, may be diminished when there is a MCL injury or absent in partial ACL tears. Experience in performing the Lachman test and in appreciating the quality of the end point will give the examiner a high degree of accuracy in making the diagnosis of an ACL tear without resorting to anesthe sia, arthroscopy, or arthrography in most patients.
Detection of Muscle Dysmorphia symptoms in male weightlifters

espanolLa Dismorfia Muscular (DM) es un desorden dismorfico corporal en el que la persona que lo padece esta insatisfecho con su tamano corporal, y/o forma. La DM no ha sido estudiada en halterofilos, por lo que el objetivo de esta investigacion fue detectar sintomas en 32 halterofilos varones, asi como caracteristicas y comportamientos asociados. Este estudio requirio la cumplimentacion de un cuestionario sociodemografico ademas de una Escala de Satisfaccion Muscular, un Cuestionario de Autoconcepto Fisico y el Somatomorpic Matrix, asi como el registro de las medidas antropometricas de los participantes. Los resultados revelaron la existencia de insatisfaccion muscular entre los deportistas ademas del uso de sustancias con el objetivo de hacer crecer su musculatura y mejorar su rendimiento, comportamientos obsesivos con el fin de comprobar su musculatura, y bajas puntuaciones en autoconcepto fisico como consecuencia de la insatisfaccion muscular que sufrian. Se puede concluir que esta investigacion confirma la existencia de DM en esta modalidad deportiva EnglishMuscle dysmorphia (MD) is a body dismorphic disorder in which the sufferer is dissatisfied with their body size and shape. MD has yet to be studied in weightlifters, and the objective of this research study is to detect its symptoms in 32 male weightlifters as well as any related characteristics and behaviours. This study involved the distribution of a sociodemographic questionnaire in addition to the Escala de Satisfaccion Muscular, the Physical Self-Concept Questionnaire, and the Somatomorphic Matrix, with the anthropometric measurements of the participants also being recorded. The results revealed the existence of body dissatisfaction among athletes and, in some cases, muscle perception that does not equate to reality. The athletes also use substances to build their muscles and enhance their performance, display obsessive muscle checking behaviours, and have low general physical self-concept that stems from their muscle (dis)satisfaction. To conclude, this study confirms the existence of MD in this sport.
Quality of Life Outcomes in a Pediatric Thalassemia Population in Egypt

Abstract Thalassemia is a disorder of hemoglobin (Hb) synthesis characterized by chronic hemolysis. In β-thalassemias major (β-TM), patients require regular transfusion at an early age due to severe anemia. Subsequently, intensive chelation therapy is initiated to mitigate the effects of the resultant iron overload. Clinical disease burden and the demanding treatment can affect health-related quality of life (HRQoL) outcomes in this population. The aim of this study was to assess HRQoL outcomes in Egyptian pediatric thalassemia patients. Patients were enrolled simultaneously from the hematology clinic at the National Research Institute in Cairo, Egypt. The Arabic version of SF36 tool was used to assess HRQoL outcomes. Socioeconomic data were collected by patient and parent interviews. Clinical data were collected by review of medical records. One hundred and thirty patients and 60 controls were enrolled, with a mean age of 5.4 ± 3.2 years and 6.3 ± 3.0, respectively. The HRQoL outcome scores were lower in all domains in the thalassemia group compared to the control group (p = 0.0001). Transfusion-dependent (TD) patients had lower HRQoL scores compared to nontransfusion-dependent (NTD) patients (p = 0.0001). Patient education and maternal education were independently associated with better HRQoL scores (p = 0.007, p = 0.028, respectively). Residents of rural areas reported lower scores compared to urban residents (p = 0.026). Thalassemia was associated with lower HRQoL scores, in all domains, compared to HRQoL in unaffected controls. Chronic transfusion independence, patient education, and maternal education were all associated with higher HRQoL scores. Psychological, social, and economic support for families with thalassemia are all essential tools to improve HRQoL outcomes.
Electronic evidence of an insulator–superconductor crossover in single-layer FeSe/SrTiO3 films

Significance The doping-induced insulator-to-superconductor transition has been widely observed in cuprates, which provides important information for understanding the superconductivity mechanism. However, in the iron-based superconductors, no evidence of doping-induced insulator–superconductor transition (or crossover) has been reported so far. In this paper, to our knowledge, we report the first electronic evidence of an insulator–superconductor crossover observed in the single-layer FeSe film grown on a SrTiO3 substrate, which exhibits similar behaviors to that observed in the cuprate superconductors. The observed insulator–superconductor crossover may be associated with the two-dimensionality that enhances electron localization or correlation. The reduced dimensionality and the interfacial effect provide a new pathway in searching for new phenomena and novel superconductors with a high transition temperature. In high-temperature cuprate superconductors, it is now generally agreed that superconductivity is realized by doping an antiferromagnetic Mott (charge transfer) insulator. The doping-induced insulator-to-superconductor transition has been widely observed in cuprates, which provides important information for understanding the superconductivity mechanism. In the iron-based superconductors, however, the parent compound is mostly antiferromagnetic bad metal, raising a debate on whether an appropriate starting point should go with an itinerant picture or a localized picture. No evidence of doping-induced insulator–superconductor transition (or crossover) has been reported in the iron-based compounds so far. Here, we report an electronic evidence of an insulator–superconductor crossover observed in the single-layer FeSe film grown on a SrTiO3 substrate. By taking angle-resolved photoemission measurements on the electronic structure and energy gap, we have identified a clear evolution of an insulator to a superconductor with increasing carrier concentration. In particular, the insulator–superconductor crossover in FeSe/SrTiO3 film exhibits similar behaviors to that observed in the cuprate superconductors. Our results suggest that the observed insulator–superconductor crossover may be associated with the two-dimensionality that enhances electron localization or correlation. The reduced dimensionality and the interfacial effect provide a new pathway in searching for new phenomena and novel superconductors with a high transition temperature.
Experimental study of an injection-locked gyrotron backward-wave oscillator.

A Ka-band gyrotron backward-wave oscillator (gyro-BWO) experiment has been performed to investigate the phase control, fast tunability, and power capability. Efficient injection locking was accomplished by novel circuit arrangements. Maximum power of 113 kW at -19% efficiency was achieved, showing favorable scaling to still higher powers. Stable voltage tuning bandwidth of 5% at 67 kW peak power was also observed. The measured performance has demonstrated a strong potential of the gyro-BWO for new applications requiring high-power tunable millimeter-wave sources
Quercetin induces insulin secretion by direct activation of L‐type calcium channels in pancreatic beta cells

Quercetin is a natural polyphenolic flavonoid that displays anti‐diabetic properties in vivo. Its mechanism of action on insulin‐secreting beta cells is poorly documented. In this work, we have analysed the effects of quercetin both on insulin secretion and on the intracellular calcium concentration ([Ca2+]i) in beta cells, in the absence of any co‐stimulating factor.
Evaluation of a line immunoblot assay for detection of antibodies recognizing extractable nuclear antigens

Sera (n = 90) giving positive results in a screening test for antibodies to extractable nuclear antigens (ENAs) were tested in a line immunoblot assay that measures antibody reactivity with individual ENAs in a single test field. Results were then compared to those obtained in monospecific ENA antibody enzyme immunoassays (EIAs). Discordant results were resolved by immunodiffusion. Of 540 result pairs (90 sera tested for 6 ENAs [Sm/RNP, Sm, SSA, SSB, Scl‐70, Jo‐1]), 509 (94%) showed concordance. Immunodiffusion resolved 28 of 31 discordant result pairs in favor of the immunoblot result. After resolution of discordant data, the immunoblot assay exhibited 100% sensitivity for all ENA antibodies except those recognizing Scl‐70, for which the sensitivity was 89%; specificity was over 96% for all 6 ENA antibodies. These findings show that a line immunoblot assay for the characterization of ENA antibodies yields results comparable to those obtained using monospecific ENA antibody EIAs. The immunoblot assay is easier and less expensive to perform due to its utilization of a single test field. J. Clin. Lab. Anal. 12:320–324, 1998. © 1998 Wiley‐Liss, Inc.
U0126 and PD98059, Specific Inhibitors of MEK, Accelerate Differentiation of RAW264.7 Cells into Osteoclast-like Cells*

Osteoclasts are multinucleated cells that differentiate from hematopoietic cells and possess characteristics responsible for bone resorption. To study the involvement of mitogen-activated protein kinases (MAPKs) in osteoclastogenesis of the murine monocytic cell line RAW264.7, which can differentiate into osteoclast-like cells in the presence of the receptor activator of nuclear factor kappa B ligand (RANKL), we treated the cells with specific inhibitors of p38 MAPK, PD169316 and SB203580, and specific inhibitors of MAPK extracellular signaling-regulated kinase (ERK) kinase (MEK), U0126 and PD98059. Each inhibitor blocked differentiation into osteoclast-like cells when the cells were plated at the standard cell density (2000–4000 cells per well (96-well)). However, the effect of MEK inhibitors on osteoclastogenesis varied according to the initial cell density during culture, because cell growth was clearly inhibited by them. When the cells were plated at more than 8000 cells per well, marked enhancement and acceleration of the differentiation were observed. In addition, immunoblot analysis revealed that phosphorylation of ERK was increased by treatment with the p38 inhibitors, whereas the MEK inhibitors increased phosphorylation of p38, which implies a seesaw-like balance between ERK and p38 phosphorylation. We suggest that osteoclastogenesis is regulated under a balance between ERK and p38 pathways and that the MEK/ERK pathway negatively regulates osteoclastogenesis while the p38 pathway does so positively. This is the first report that an inhibitor of signal transduction enhanced osteoclastogenesis.