Miracle-dz/UniFAR-FTUs huggingface dataset:
Validity and reliability of the assessment and diagnosis of musculoskeletal elbow disorders using telerehabilitation

We examined the validity and reliability of a physiotherapy examination of the elbow, using telerehabilitation. The patho-anatomical diagnoses, systems diagnosis and physical examination findings of face-to-face physiotherapy examinations were compared with telerehabilitation examinations. Ten participants attended a single session, during which they were interviewed, a face-to-face physical examination was performed and a remote physical examination was conducted, guided by an examiner at a different location via a telerehabilitation system. Conventional face-to-face physiotherapy physical examination test results, diagnoses and systems diagnoses were compared to those produced by an examiner using the telerehabiliation system. There was substantial agreement for systems diagnosis (73%; P = 0.013) for validity and almost perfect agreement for intra-rater reliability (90%; P = 0.001). The inter-rater reliability had a weaker and non-significant agreement (64%; P = 0.11). Physical examination data demonstrated >68% agreement across all three datasets, between the examination methods. Performing a telerehabilitation physical examination to determine a musculoskeletal diagnosis of the elbow joint complex is both valid and reliable.
Effect of LAK cells against three-dimensional tumor tissue. In vitro study using multi-cellular human glioma spheroids as targets.

The anti-tumor mechanisms of local LAK cell therapy are difficult to study in vivo. We describe a method to study in vitro the action of LAK cells against three-dimensional tumor tissue. Spherical cell aggregates (spheroids) grown from human glioma cell lines H-2 and U-251 were labeled with 51Cr and then incubated for up to 24 h with LAK cells. After the incubation, most spheroids were still macroscopically identifiable, and the measured reduction of volume did not correlate to the extent of damage. LAK cells infiltrated into spheroid tissue slowly as a frontier which explains why the specific 51Cr release was clearly slower from spheroids than corresponding single cell suspensions. The infiltrated area was at 1 to 2 h very thin but by 8 to 12 h consisted already of several cell layers. Most H-2 spheroids became totally infiltrated by 16 to 24 h whereas in U-251 spheroids the infiltration usually remained peripheral. In accordance with the different extent of infiltration, H-2 spheroids were clearly more sensitive to LAK cells than U-251 spheroids: at E/T ratio 10:1 the mean specific 51Cr release by 24 h was 63 and 36%, respectively. A single exposure to LAK cells released 51Cr from H-2 spheroids approximately 12 h but over 24 h from U-251 spheroids. The spheroid model can be used to study the infiltrative capacity and cytotoxicity of LAK cells against three-dimensional tumor tissue, and the method may help to find an optimal mode of local LAK cell therapy, i.e., proper combination of lymphokines and LAK cells, and proper timing of their administration.
Psychosocial predictors of posttreatment pain after nonmetastatic breast cancer treatment: a systematic review and meta-analysis of prospective studies

Background The search for risk factors of pain after breast cancer, which affects a considerable proportion of the women, has primarily focused on clinical factors. The aim of this meta-analysis was to explore the less well-studied psychosocial predictors of pain after breast cancer treatment. Methods Two independent searches were conducted in PubMed, PsycINFO, Web of Science, and CINAHL. Eligible studies were prospective, observational studies of women aged ≥18 years, diagnosed and treated for nonmetastatic breast cancer ≥3 months previously. Additional inclusion criteria were that studies had assessed at least one pain outcome and at least one psychosocial predictor. The psychosocial predictors investigated included: 1) psychological–behavioral states, 2) psychological traits, and 3) social support. Effect size correlations (ESr) were chosen as the effect size and pooled using a random effects model. We also explored a number of study characteristics as possible moderators of the effect with meta-regression. Results Of the total of 13 eligible studies identified, most studies measured psychosocial predictors at presurgery. Neither psychological–behavioral states (ESr: 0.05; p=0.13; K=11) nor psychological traits (ESr: 0.02; p=0.48; K=6) emerged as statistically significant predictors of pain. In contrast, higher levels of social support were statistically significantly associated with less pain (ESr: −0.24; p<0.001; K=4). In studies of psychological–behavioral states, longer follow-up was associated with smaller effect sizes (p=0.023). Furthermore, older mean sample age was associated with larger effect sizes for both psychological–behavioral states (p=0.0004) and psychological traits (p=0.035). Conclusion The results of this meta-analysis suggest that psychosocial factors measured at presurgery may only be of modest predictive value in identifying women at risk of developing pain after breast cancer treatment. While speculative, psychosocial factors may play a larger role in the postsurgery trajectory, which could be valuable to investigate in future studies.
IL-8 Decreases HIV-1 Transcription in Peripheral Blood Lymphocytes and Ectocervical Tissue Explants

IL-8 is enhanced in the peripheral blood and lymphoid tissue of HIV-infected individuals, suggesting that IL-8 is important in the pathogenesis of HIV-1 infection and progression to AIDS. Characterizing the mechanisms of IL-8 regulation of HIV-1 replication may be relevant in addressing the role of IL-8 as a therapeutic target in HIV-1 infection. We evaluated replication of primary R5-tropic HIV-1 in peripheral blood lymphocytes and ectocervical tissue explants infected in vitro in the presence of physiological concentrations of IL-8 found in the serum and genital tract secretions of HIV-infected individuals. To identify the specific stages of the viral life cycle targeted by IL-8, we performed real-time polymerase chain reaction to detect HIV-1 reverse transcription, integration, and transcription. Early during the infection, IL-8 decreased HIV-1 reverse transcription and viral integration. This effect was transient, as on day 4 after infection, we detected no differences on HIV-1 DNA or proviral DNA in peripheral blood lymphocyte. IL-8 decreased HIV-1 transcription in both lymphocytes and ectocervical tissue explants. The decrease in viral RNA expression was associated with reduced HIV-1 replication, as measured by viral p24 release in the culture supernatants. This is the first report to suggest that IL-8 decreases replication of primary R5-tropic HIV-1 by transcriptional mechanisms.
Rationally Controlled Synthesis of CdSexTe1−x Alloy Nanocrystals and Their Application in Efficient Graded Bandgap Solar Cells

CdSexTe1−x semiconductor nanocrystals (NCs), being rod-shaped/irregular dot-shaped in morphology, have been fabricated via a simple hot-injection method. The NCs composition is well controlled through varying molar ratios of Se to Te precursors. Through changing the composition of the CdSexTe1−x NCs, the spectral absorption of the NC thin film between 570–800 nm is proved to be tunable. It is shown that the bandgap of homogeneously alloyed CdSexTe1−x active thin film is nonlinearly correlated with the different compositions, which is perceived as optical bowing. The solar cell devices based on CdSexTe1−x NCs with the structure of ITO/ZnO/CdSe/CdSexTe1−x/MoOx/Au and the graded bandgap ITO/ZnO/CdSe(w/o)/CdSexTe1−x/CdTe/MoOx/Au are systematically evaluated. It was found that the performance of solar cells degrades almost linearly with the increase of alloy NC film thickness with respect to ITO/ZnO/CdSe/CdSe0.2Te0. 8/MoOx/Au. From another perspective, in terms of the graded bandgap structure of ITO/ZnO/CdSe/CdSexTe1−x/CdTe/MoOx/Au, the performance is improved in contrast with its single-junction analogues. The graded bandgap structure is proved to be efficient when absorbing spectrum and the solar cells fabricated under the structure of ITO/ZnO/CdSe0.8Te0. 2/CdSe0.2Te0. 8/CdTe/MoOx/Au indicate power conversion efficiency (PCE) of 6.37%, a value among the highest for solution-processed inversely-structured CdSexTe1−x NC solar cells. As the NC solar cells are solution-processed under environmental conditions, they are promising for fabricating solar cells at low cost, roll by roll and in large area.
Commentary on “Consensus Recommendations on Sulfonylurea and Sulfonylurea Combinations in the Management of Type 2 Diabetes Mellitus: International Task Force”

Sulfonylureas (SUs) have been in use for treatment of type 2 diabetes mellitus (DM) for more than 50 years. The confidence developed with their use in controlling blood glucose to target has been tainted by its untoward effect of hypoglycemia. The newer oral agents have emerged as an alternative to SUs, and the risk of hypoglycemia is no more concern but the efficacy and cost of treatment. Additional benefits such as sustenance of effects and passing the test of cardiac safety are justification for preference of these agents over SUs. Despite a long track record of clinical use of SUs, their cardiac safety has always been a concern due to the paucity of studies designed to address this issue. Cardiac safety of SUs is not feasible to test in randomized controlled trials (RCTs) now, and it is left to resort to analysis of clinical data of the previous studies for safety issues. Efficacy and economical benefit of SUs with unclear cardiac risk continue to place the drug in the choice ladder of oral antidiabetic agents with variable positioning. Known cardiovascular risk factors such as weight gain, fluid retention, and hypoglycemia, are inherent problem with SUs. [1] In contrast, meta-analyses of sulfonylurea randomized controlled trials (RCTs) have produced conflicting findings with respect to cardiovascular events and mortality. [2-4] However, none of these were designed or powered to detect cardiovascular events, and the RCTs used different comparators.
Cognitive effects of head‐movements in stray fields generated by a 7 Tesla whole‐body MRI magnet

The study investigates the impact of exposure to the stray magnetic field of a whole‐body 7 T MRI scanner on neurobehavioral performance and cognition. Twenty seven volunteers completed four sessions, which exposed them to ∼1600 mT (twice), 800 mT and negligible static field exposure. The order of exposure was assigned at random and was masked by placing volunteers in a tent to hide their position relative to the magnet bore. Volunteers completed a test battery assessing auditory working memory, eye–hand co‐ordination, and visual perception. During three sessions the volunteers were instructed to complete a series of standardized head movements to generate additional time‐varying fields (∼300 and ∼150 mT·s−1 r.m.s.). In one session, volunteers were instructed to keep their heads as stable as possible. Performance on a visual tracking task was negatively influenced (P<.01) by 1.3% per 100 mT exposure. Furthermore, there was a trend for performance on two cognitive‐motor tests to be decreased (P<.10). No effects were observed on working memory. Taken together with results of earlier studies, these results suggest that there are effects on visual perception and hand–eye co‐ordination, but these are weak and variable between studies. The magnitude of these effects may depend on the magnitude of time‐varying fields and not so much on the static field. While this study did not include exposure above 1.6 T, it suggests that use of strong magnetic fields is not a significant confounder in fMRI studies of cognitive function. Future work should further assess whether ultra‐high field may impair performance of employees working in the vicinity of these magnets. Bioelectromagnetics 28:247–255, 2007. © 2007 Wiley‐Liss, Inc.
Effects of Warming-up on Physical Performance: A Systematic Review With Meta-analysis

Fradkin, AJ, Zazryn, TR, and Smoliga, JM. Effects of warming-up on physical performance: a systematic review with meta-analysis. J Strength Cond Res 24(1): 140-148, 2010-The value of warming-up is a worthy research problem because it is not known whether warming-up benefits, harms, or has no effect on individuals. The purpose of this study was to review the evidence relating to performance improvement using a warm-up. A systematic review and meta-analysis were undertaken. Relevant studies were identified by searching Medline, SPORTDiscus, and PubMed (1966-April 2008). Studies investigating the effects of warming-up on performance improvement in physical activities were included. Studies were included only if the subjects were human and only if the warm-up included activities other than stretching. The quality of included studies was assessed independently by 2 assessors using the Physiotherapy Evidence Database scale. Thirty-two studies, all of high quality (6.5-9 [mean = 7.6] of 10) reported sufficient data (quality score >6) on the effects of warming-up on performance improvement. Warm-up was shown to improve performance in 79% of the criterions examined. This analysis has shown that performance improvements can be demonstrated after completion of adequate warm-up activities, and there is little evidence to suggest that warming-up is detrimental to sports participants. Because there were few well-conducted, randomized, controlled trials undertaken, more of these are needed to further determine the role of warming-up in relation to performance improvement.
Native pipefish Entelurus aequoreus are promoted by the introduced seaweed Sargassum muticum in the northern Wadden Sea, North Sea

After the major loss of eelgrass habitats in the 1930s the snake pipefish Entelurus aequoreus was considered to be rare in the Wadden Sea (south-eastern North Sea, German Bight). We hypothesize that a sudden increase in pipefish abundance observed in the area since 2004 is related to new habitats provided by the introduced Japanese seaweed Sargassum muticum. SCUBA observations conducted near the Island of Sylt during July 2006 showed that approximately every second S. muticum thallus was inhabited by E. aequoreus, whereas no pipefish could be found on sedimentary areas dominating the Wadden Sea. In a field experiment, we removed thalli from a S. muticum bed and transplanted them to an unvegetated site and to the donor S. muticum bed, respec- tively. Nine days after the transplantation, abundances of pipefish per thallus were almost equally high within all transplanted S. muticum units at vegetated and originally unvegetated sites. Abun- dances of E. aequoreus in transplanted thalli did not significantly differ from those found in unaf- fected control thalli located in the donor S. muticum bed. To explain high pipefish densities within S. muticum beds we sampled the zooplankton community inside and outside the S. muticum bed and compared it with gut contents of pipefish. Zooplankton densities were significantly higher inside the S. muticum bed. Harpacticoid copepods were the dominant group associated with S. muticum and also represented the major prey of E. aequoreus. We conclude that stocks of E. aequoreus are pro- moted by S. muticum beds since they benefit from higher food supply during the spawning season, probably in addition to increased shelter by habitat complexity. Results of the present study suggest that introductions of habitat-forming species such as S. muticum may cause beneficial effects on abundance and diversity of native species.
Investigating perceived barriers to the use of open educational resources in higher education in Tanzania

The past few years have seen increasingly rapid development and use of open educational resources (OER) in higher education institutions (HEIs) in developing countries. These resources are believed to be able to widen access, reduce the costs, and improve the quality of education. However, there exist several challenges that hinder the adoption and use of these resources. The majority of challenges mentioned in the literature do not have empirically grounded evidence and they assume Sub-Saharan countries face similar challenges. Nonetheless, despite commonalities that exist amongst these countries, there also exists considerable diversity, and they face different challenges. Accordingly, this study investigated the perceived barriers to the use of OER in 11 HEIs in Tanzania. The empirical data was generated through semi-structured interviews with a random sample of 92 instructors as well as a review of important documents. Findings revealed that lack of access to computers and the Internet, low Internet bandwidth, absence of policies, and lack of skills to create and/or use OER are the main barriers to the use of OER in HEIs in Tanzania. Contrary to findings elsewhere in Africa, the study revealed that lack of trust in others’ resources, lack of interest in creating and/or using OER, and lack of time to find suitable materials were not considered to be barriers. These findings provide a new understanding of the barriers to the use of OER in HEIs and should therefore assist those who are involved in OER implementation to find mitigating strategies that will maximize their usage.
African meliponine bees (Hymenoptera: Apidae) maintained in man-made hives as potential hosts for the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae)

Previous studies have shown that natural honeybee and bumble bee colonies are hosts of the small hive beetle (SHB) Aethina tumida, a pest of honeybee colonies in various regions of the world. Recent studies also reported the presence of SHBs in colonies of certain meliponine bee species. In this study, we investigated whether SHBs detect odors of African meliponine bees and their hive matrix components. We also compared the chemical profiles of the honeybee Apis mellifera scutellata and meliponine bee odors in order to identify common potential semiochemicals between the two bee species. We used dual-choice olfactometric assays to test the responses of adult male and female SHBs to intact colony odors from six meliponine bee species, namely Hypotrigona gribodoi, Meliponula ferruginea (black), M. ferruginea (reddish-brown), Plebeina hildbrandti, M. bocandei and M. lendiliana and their hive matrix components including pot honey, pot pollen, cerumen (involucrum) and propolis (batumen). We found that female SHBs responded more strongly to odors from intact colonies, pot honey and pollen from five out of the six species tested than male beetles. Chemical analysis identified several common components in colony odors emitted by both honeybees and a representative meliponine bee species, M. ferruginea (black). In particular, nine of these common components previously have been shown in honeybee volatiles to be semiochemicals for the SHB, suggesting that African meliponine bees can also serve as potential alternate hosts for the beetle. The implications of these results are discussed in the context of domesticating African meliponine bees in man-made hives for the pollination of crops.
Transforming the delivery of health and social care: the case for fundamental change.

The majority of my workload at the minute as a GP trainee in a busy maternity unit is the diametric opposite to the care we give people at the end of their lives. Babies are planned for meticulously, their birth plans are discussed with their parents, and the mother is meticulously screened to ensure that the pregnancy has every chance of succeeding. People decide how and when they are to attend hospital, in what circumstances they will deliver, the …
Template‐free generation of RNA species that replicate with bacteriophage T7 RNA polymerase.

A large variety of different RNA species that are replicated by DNA‐dependent RNA polymerase from bacteriophage T7 have been generated by incubating high concentrations of this enzyme with substrate for extended time periods. The products differed from sample to sample in molecular weight and sequence, their chain lengths ranging from 60 to 120. The mechanism of autocatalytic amplification of RNA by T7 RNA polymerase proved to be analogous to that observed with viral RNA‐dependent RNA polymerases (replicases): only single‐stranded templates are accepted and complementary replica strands are synthesized. With enzyme in excess, exponential growth was observed; linear growth resulted when the enzyme was saturated by RNA template. The plus strands, present at 90% of the replicating RNA species, were found to have GG residues at both termini. Consensus sequences were not found among the sequences of the replicating RNA species. The secondary structures of all species sequenced turned out to be hairpins. The RNA species were specifically replicated by T7 RNA polymerase; they were not accepted as templates by the RNA polymerases from Escherichia coli or bacteriophage SP6 or by Qbeta replicase; T3 RNA polymerase was partially active. Template‐free production of RNA was completely suppressed by addition of DNA to the incubation mixture. When both DNA and RNA templates were present, transcription and replication competed, but T7 RNA polymerase preferred DNA as a template. No replicating RNA species were detected in vivo in cells expressing T7 RNA polymerase.
A 12-month follow-up study of the course of body dysmorphic disorder.

OBJECTIVE This study investigated the course of body dysmorphic disorder (BDD), a relatively common and severe disorder, in the first prospective follow-up study, to the authors' knowledge. METHOD In this study, the authors obtained data with the Longitudinal Interval Follow-Up Evaluation on weekly BDD symptom status and treatment received over 1 year for 183 broadly ascertained subjects. Probabilities of full remission, partial remission, and relapse during this year were examined. Full remission was defined as minimal or no BDD symptoms and partial remission, as meeting less than full DSM-IV criteria for at least 8 consecutive weeks. Relapse was defined as meeting full BDD criteria for at least 2 consecutive weeks after attaining partial or full remission from BDD. RESULTS Over 1 year, the probability of full remission from BDD was only 0.09, and the probability of partial remission was 0.21. Although 84.2% of the subjects received mental health treatment during the 1-year period, mean BDD severity scores during the year reflected full DSM-IV criteria for BDD, and the mean proportion of time that the subjects met full BDD criteria was 80%. Gender and ethnicity did not significantly predict remission from BDD. Among the subjects whose BDD symptoms partially or fully remitted, the probability of relapse was 0.15. CONCLUSIONS These findings indicate that BDD tends to be chronic. Remission probabilities were lower than reported for mood disorders, most anxiety disorders, and personality disorders in studies with similar methods.