Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of the time required to perform three retreatment techniques with dental microscope and ultrasonic activation for removing filling material from the oval root canal

Background When endodontic treatment fails, retreatment consists of the complete removal of the root canal filling material for thorough cleaning and reobturation. Various techniques are available for the filling removal procedure, which present varying degrees of efficacy, and take a varying length of time to perform. The aim of this study was to compare the time required to carry out reciprocating, rotary, and manual techniques with dental microscope and ultrasonic activation for removing filling material from root canals. Material and Methods Ninety-nine extracted single-rooted teeth with straight and oval-shaped canals were selected. The samples were instrumented with ProTaper Gold System up to file F2 and obturated with AH Plus sealer and GuttaCore. The teeth were randomly divided into three groups (n=33) according to the instruments used for removal of filling material: Group (1) Reciproc blue R50 instrument; Group (2) ProTaper Retreatment instruments; Group (3) manual (Hedstrom files and Gates-Glidden drills), under dental microscope at X10 magnification with Ultrasonic retreatment tip. The time required to remove the filling materials was measured with a chronometer. Data were analyzed statistically applying the Kruskal-Wallis test followed by the Mann-Whitney U-test (p<0.05). Results The time required to remove filling material was significantly shorter in Group 1, followed by Group 2, the slowest being Group 3 (P<0.05). Conclusions The reciprocating technique was the fastest method for removing root canal filling material. Key words:Endodontic retreatment, hedstrom file, ProTaper retreatment, reciproc blue.
Assessing mechanisms of benefit in adult day programs: the adult day services process and use measures

Abstract Objectives: A limitation of adult day service (ADS) research is that there remains little understanding of how these community-based long-term care programs operate to benefit clients or family caregivers (i.e. the process of ADS use). The purpose of this study was to validate the ‘ADS Process and Use Measures’ (APUM) which were developed to assess such mechanisms. Method: Participant observation and semi-structured interviews in two ADS settings resulted in qualitative data to inform a conceptual model, subscales, and Likert-scale items. Three experts in ADS research reviewed the initial 129-item version of the APUM to establish content validity, and 27 family caregivers of current or prior ADS clients provided feedback on face validity of a subsequent 58-item version. Results: Principal components and confirmatory factor analyses on a sample of 269 family members of ADS clients recruited from 90 programs throughout the U.S. established a measure featuring 5 domains, 12 reliable subscales, and 49 items. Analysis of discriminant and convergent validity found that various subscales from four of the domains (Why ADS is Used, Events Prior to Use, Why ADS Does Not Work, and Pathways to Benefits) were significantly associated (p < 0.05) with family caregiver distress and ADS client quality of life variables. Conclusion: The ADS Process and Use Measures effectively assess mechanisms of program benefit and could help to enhance the overall quality of these critical community-based long-term care options for older persons and their families.
Oldest skeleton of a fossil flying squirrel casts new light on the phylogeny of the group

Flying squirrels are the only group of gliding mammals with a remarkable diversity and wide geographical range. However, their evolutionary story is not well known. Thus far, identification of extinct flying squirrels has been exclusively based on dental features, which, contrary to certain postcranial characters, are not unique to them. Therefore, fossils attributed to this clade may indeed belong to other squirrel groups. Here we report the oldest fossil skeleton of a flying squirrel (11.6 Ma) that displays the gliding-related diagnostic features shared by extant forms and allows for a recalibration of the divergence time between tree and flying squirrels. Our phylogenetic analyses combining morphological and molecular data generally support older dates than previous molecular estimates (~23 Ma), being congruent with the inclusion of some of the earliest fossils (~36 Ma) into this clade. They also show that flying squirrels experienced little morphological change for almost 12 million years.
The structure of the ovarian ball and oogenesis in Moniliformis dubius (Acanthocephala)

Seven to nine days after infection of the definitive host (rat) by cystacanths, the genital primordium of the female acanthocephalan is transformed from a fragmented mass of cells into discrete ovarian balls. This is accomplished by envelopment of free germinal cells by somatic tissue which originates from the ligament sac primordium. Germinal cell nuclei then undergo repeated mitoses until about 21 days of development, with concurrent formation of oogonial syncytia which occupy the interior of the ovarian balls. Oocytes, derived from these oogonia, move to the periphery of the germinal syncytia for differentiation, growth, fertilization, shell formation, and release from the ovarian ball. After oogonial proliferation ceases, continued growth of the ovarian ball apparently results from increase in size of already present cells.
Does the Improvement of Position Sense Following Motor Imagery Practice Vary as a Function of Age and Time of Day?

ABSTRACT The effectiveness of motor imagery practice is known to depend on age and on the ability to form motor images. In the same individual, motor imagery quality changes during the day, being better late in the morning for older adults and in the afternoon for younger adults. Does this mean that motor imagery practice should be done at specific time of the day depending on the age of participants to maximize motor learning? To examine whether the effect of motor imagery practice varies as a function of time of day and age, the authors used an arm configuration reproduction task and measured position sense accuracy before and after 135 kinesthetic motor imagery trials. Younger and older participants were randomly assigned to either a morning or an afternoon session. Data showed that the accuracy for reproducing arm configurations improved following imagery practice regardless of time of day for both younger and older adults. Moreover, the authors observed that the position sense was less accurate in the afternoon than in the morning in older participants (before and after motor imagery practice), while performance did not change during the day in younger participants. These results may have practical implications in motor learning and functional rehabilitation programs. They highlight the effectiveness of motor imagery practice for movement accuracy in both younger and older adults regardless of time of day. By contrast, they reveal that the assessment of position sense requires that the time of day be taken into account when practitioners want to report on the older patients’ progress without making any mistakes.
Selective Effects of the Loss of NMDA or mGluR5 Receptors in the Reward System on Adaptive Decision-Making

Visual Abstract Selecting the most advantageous actions in a changing environment is a central feature of adaptive behavior. The midbrain dopamine (DA) neurons along with the major targets of their projections, including dopaminoceptive neurons in the frontal cortex and basal ganglia, play a key role in this process. Here, we investigate the consequences of a selective genetic disruption of NMDA receptor and metabotropic glutamate receptor 5 (mGluR5) in the DA system on adaptive choice behavior in mice. We tested the effects of the mutation on performance in the probabilistic reinforcement learning and probability-discounting tasks. In case of the probabilistic choice, both the loss of NMDA receptors in dopaminergic neurons or the loss mGluR5 receptors in D1 receptor-expressing dopaminoceptive neurons reduced the probability of selecting the more rewarded alternative and lowered the likelihood of returning to the previously rewarded alternative (win-stay). When observed behavior was fitted to reinforcement learning models, we found that these two mutations were associated with a reduced effect of the expected outcome on choice (i.e., more random choices). None of the mutations affected probability discounting, which indicates that all animals had a normal ability to assess probability. However, in both behavioral tasks animals with targeted loss of NMDA receptors in dopaminergic neurons or mGluR5 receptors in D1 neurons were significantly slower to perform choices. In conclusion, these results show that glutamate receptor-dependent signaling in the DA system is essential for the speed and accuracy of choices, but at the same time probably is not critical for correct estimation of probable outcomes.
Barred Pipe Modification - Does It Affect the Learning Results?

: This research aims to find out about the effect of basketball learning modification on ‘layup shot’ learning result. The method used in this research is the experimental method, with the research design of ‘Randomize Pre-test – Post-test Control Group Design’. The population in this research consists of 11th graders from SMAN 1 Sindangwangi, consisting of 103 students from four different classes. The sample in this research was acquired using ‘cluster random sampling’ method. The sample acquired consisted of 50 students from two different classes, each class consisted of 25 students. One class becomes an experimental group, and the other one becomes the control group. The instrument in this research is ‘layup shot’ test. This research was conducted for four weeks; three meetings were done each every week. The data analysis technique is SPSS 18 through ‘Paired Samples t-Test’ and ‘Independent t-Test’. The outputs from this research are: 1) There’s a significant increase of score between the pre-test and post-test score on the students’ layup shot learning result that used the barred pipe modification learning; 2) There was no significant increase in score between the pre-test score and post-test score on the layup shot learning result of the students that used the conventional learning.
Additional Biomarkers beyond RAS That Impact the Efficacy of Cetuximab plus Chemotherapy in mCRC: A Retrospective Biomarker Analysis

Purpose We aimed to identify new predictive biomarkers for cetuximab in first-line treatment for patients with RAS wild-type metastatic colorectal cancer (mCRC). Methods The study included patients with KRAS wild-type unresectable liver-limited mCRC treated with chemotherapy with or without cetuximab. Next-generation sequencing was done for single nucleotide polymorphism according to custom panel. Potential predictive biomarkers were identified and integrated into a predictive model within a training cohort. The model was validated in a validation cohort. Results Thirty-one of 247(12.6%) patients harbored RAS mutations. In training cohort (N=93), six potential predictive genes, namely, ATP6V1B1, CUL9, ERBB2, LY6G6D, PTCH1, and RBMXL3, were identified. According to predictive model, patients were divided into responsive group (n=66) or refractory group (n=27). In responsive group, efficacy outcomes were significantly improved by addition of cetuximab to chemotherapy. In refractory group, no benefit was observed. Interaction test was significant across all endpoints. In validation cohort (N=123), similar results were also observed. Conclusions In the first-line treatment of mCRC, the predictive model integrating six new predictive mutations divided patients well, indicating a promising approach to further refine patient selection for cetuximab on the basis of RAS mutations.
Anaesthesia care team improves outcomes in surgical patients compared with solo anaesthesiologist: An observational study

BACKGROUND In anaesthesiology, little attention has been drawn to the role of anaesthesia nurses as support personnel on quality of care. OBJECTIVES To compare an anaesthesiologist alone (solo anaesthesiologist) with an anaesthesia care team (anaesthesiologist and anaesthesia nurse). DESIGN An observational study. SETTING A single centre study. PARTICIPANTS Anaesthesiologists and anaesthesia nurses. INTERVENTION Anaesthesia performed by solo anaesthesiologists compared with anaesthesia care teams. MAIN OUTCOME MEASURES 30-day postoperative mortality and hospital length of stay. Propensity score matching was performed by logistic regression to adjust for baseline differences between the two groups and pairs of perfectly matched patients were formed. RESULTS Anaesthesia was performed by solo anaesthesiologists in 2832 patients and by an anaesthesia care team in 2842 patients. Matching with 2095 pairs of perfectly matched patients was formed. The two groups were comparable in respect of sex and duration of anaesthesia but differed notably for age, American Society of Anesthesiologists’ physical status score and type of surgery. Logistic regression showed a significantly lower 30-day mortality rate for the anaesthesia care teams compared with solo anaesthesiologists (0.76 vs. 1.56%, P = 0.0014). Length of hospital stay was also significantly reduced when an anaesthesia nurse was present (4.9 ± 10.1 vs. 5.6 ± 11.5 days, P = 0.0011). CONCLUSION Anaesthesia given by teams of anaesthesiologists and anaesthesia nurses is associated with decreased 30-day postoperative mortality and shorter length of stay when compared with solo anaesthesiologists. Even without any demonstration of causality, this emphasises the benefits of the anaesthesia care team model. TRIAL REGISTRATION CCB 325201730849.
Monocytes of Different Subsets in Complexes with Platelets in Patients with Myocardial Infarction

Abstract Acute myocardial infarction (AMI) is associated with activation of various cells, including platelets that form monocyte–platelet complexes (MPCs). Here, we analysed MPC in vivo and in vitro and investigated the abilities of different monocyte subclasses to form MPC, the characteristics of the cells involved in MPC formation and MPC changes in AMI. We identified MPC by co-staining for platelet antigen CD41a and monocyte antigens CD14 and CD16. Platelet activation was evaluated from expression of phosphatidylserine as revealed by annexin V. Our results confirm published data and provide new information regarding the patterns of MPC in AMI patients. We found that the patterns of platelet aggregation with monocytes were different in AMI patients and controls: (1) in AMI patients, MPC formed by intermediate monocytes carry more platelets whereas in healthy controls more platelets aggregated with classical monocytes; (2) the numbers of MPC in AMI patients, being already higher than in controls, were further increased if these patients suffered various in-hospital complications; (3) on the basis of the CD41a fluorescence of the antibody-stained MPC, some of the aggregates seem to consist of monocytes and platelet-derived extracellular vesicles (EVs); (4) aggregation of monocytes with platelet EV occurred in in vitro experiments; and (5) these experiments demonstrated that monocytes from AMI patients aggregate with both platelets and platelet EVs more efficiently than do monocytes from controls. MPC in AMI patients may play an important role in this pathology.
Kinetic properties of persistent Na+ current orchestrate oscillatory bursting in respiratory neurons

The rhythmic pattern of breathing depends on the pre-Bötzinger complex (preBötC) in the brainstem, a vital circuit that contains a population of neurons with intrinsic oscillatory bursting behavior. Here, we investigate the specific kinetic properties that enable voltage-gated sodium channels to establish oscillatory bursting in preBötC inspiratory neurons, which exhibit an unusually large persistent Na+ current (INaP). We first characterize the kinetics of INaP in neonatal rat brainstem slices in vitro, using whole-cell patch-clamp and computational modeling, and then test the contribution of INaP to rhythmic bursting in live neurons, using the dynamic clamp technique. We provide evidence that subthreshold activation, persistence at suprathreshold potentials, slow inactivation, and slow recovery from inactivation are kinetic features of INaP that regulate all aspects of intrinsic rhythmic bursting in preBötC neurons. The slow and cumulative inactivation of INaP during the burst active phase controls burst duration and termination, while the slow recovery from inactivation controls the duration of the interburst interval. To demonstrate this mechanism, we develop a Markov state model of INaP that explains a comprehensive set of voltage clamp data. By adding or subtracting a computer-generated INaP from a live neuron via dynamic clamp, we are able to convert nonbursters into intrinsic bursters, and vice versa. As a control, we test a model with inactivation features removed. Adding noninactivating INaP into nonbursters results in a pattern of random transitions between sustained firing and quiescence. The relative amplitude of INaP is the key factor that separates intrinsic bursters from nonbursters and can change the fraction of intrinsic bursters in the preBötC. INaP could thus be an important target for regulating network rhythmogenic properties.
Inter-BMV: Interpolation with Block Motion Vectors for Fast Semantic Segmentation on Video

Models optimized for accuracy on single images are often prohibitively slow to run on each frame in a video. Recent work exploits the use of optical flow to warp image features forward from select keyframes, as a means to conserve computation on video. This approach, however, achieves only limited speedup, even when optimized, due to the accuracy degradation introduced by repeated forward warping, and the inference cost of optical flow estimation. To address these problems, we propose a new scheme that propagates features using the block motion vectors (BMV) present in compressed video (e.g. H.264 codecs), instead of optical flow, and bi-directionally warps and fuses features from enclosing keyframes to capture scene context on each video frame. Our technique, interpolation-BMV, enables us to accurately estimate the features of intermediate frames, while keeping inference costs low. We evaluate our system on the CamVid and Cityscapes datasets, comparing to both a strong single-frame baseline and related work. We find that we are able to substantially accelerate segmentation on video, achieving near real-time frame rates (20+ frames per second) on large images (e.g. 960 x 720 pixels), while maintaining competitive accuracy. This represents an improvement of almost 6x over the single-frame baseline and 2.5x over the fastest prior work.
Congestive heart failure in older adults diagnosed with follicular lymphoma: A population‐based study

To the authors’ knowledge, there is limited information regarding the long‐term risk of congestive heart failure (CHF) among patients with follicular lymphoma, a prevalent non‐Hodgkin lymphoma diagnosis among those aged >65 years, especially within the context of therapeutic exposures and preexisting comorbidities.
Decreased RORC expression and downstream signaling in HTLV‐1‐associated adult T‐cell lymphoma/leukemia uncovers an antiproliferative IL17 link: A potential target for immunotherapy?

Retinoic acid-related drugs have shown promising pre-clinical activity in Adult T-cell Leukemia/Lymphoma (ATL), but RORC (Retinoic acid Orphan Receptor C) signaling has not been explored. Therefore, we investigated transcriptome-wide interactions of the RORC pathway in Human T-cell Leukemia Virus-1 (HTLV-1) infection and ATL, using our own and publicly available gene expression data for ATL and other leukemias, HTLV-1-infected individuals and healthy controls. Gene expression data from ATL patients were analyzed using Weighted Gene Correlation Network Analysis (WGCNA) to determine gene modules and their correlation to clinical and molecular data. Both PBMCs and CD4+ T-cells showed decreased RORC expression in four different ATL cohorts. A small subset of RORChi ATL patients was identified with significantly lower pathognomonic CADM1 and HBZ levels but similar levels of other ATL markers (CD4/CD25/CCR4), hinting at a less aggressive ATL subtype. In addition, an age-dependent decrease in RORC expression was found in HTLV-1-infected individuals, but not in healthy controls, suggesting an early molecular event predisposing to leukemogenesis. Genes upstream of RORC signaling were members of a proliferative gene module (containing proliferation markers PCNA/MKI67), whereas downstream members clustered in an antiproliferative gene module. IL17C transcripts showed the strongest negative correlation to PCNA in both ATL cohorts, which was replicated in two large cohorts of T- and B-cell acute leukemias. In conclusion, decreased RORC expression and downstream signaling might represent an early event in ATL pathogenesis. An antiproliferative IL17C/PCNA link is shared between ATL, T-ALL and B-ALL, suggesting (immuno)therapeutic benefit of boosting RORC/IL17 signaling. Abbreviations HTLV-1 Human T-cell Leukemia Virus-1 ATL Adult T-cell Leukemia/Lymphoma WGCNA Weighted Gene Correlation Network Analysis ATRA All-trans retinoic acid RARa Retinoic acid receptor alpha RORC Retinoic acid orphan receptor C TBLV Type B Leukomogenic Virus AML Acute Myeloid Leukemia T-ALL T-cell acute lymphoblastic leukemia IL Interleukin PCNA Proliferating cell nuclear antigen ID Infectious Dermatitis
Diabetic Enteropathy: From Molecule to Mechanism-Based Treatment

The incidence of the micro- and macrovascular complications of diabetes is rising, mirroring the increase in the worldwide prevalence. Arguably, the most common microvascular complication is neuropathy, leading to deleterious changes in both the structure and function of neurons. Amongst the various neuropathies with the highest symptom burden are those associated with alterations in the enteric nervous system, referred to as diabetic enteropathy. The primary aim of this review is to provide a contemporaneous summary of pathophysiology of diabetic enteropathy thereby allowing a “molecule to mechanism” approach to treatment, which will include 4 distinct aspects. Firstly, the aim is to provide an overview of the diabetes-induced structural remodelling, biochemical dysfunction, immune-mediated alterations, and inflammatory properties of the enteric nervous system and associated structures. Secondly, the aim is to provide a synopsis of the clinical relevance of diabetic enteropathy. Thirdly, the aim is to discuss the various patient-reported outcome measures and the objective modalities for evaluating dysmotility, and finally, the aim is to outline the clinical management and different treatment options that are available. Given the burden of disease that diabetic enteropathy causes, earlier recognition is needed allowing prompt investigation and intervention, which may lead to improvements in quality of life for sufferers.