Miracle-dz/UniFAR-FTUs huggingface dataset:
Determination of appropriate growth medium for production of sclerotia in Pleurotus tuber-regium

This study determined appropriate growth medium for production of sclerotia in Pleurotus tuber-regium. Due to climate change and other anthropogenic activities, such as forest depletion, sclerotia sourcing form the wild is tending towards extinction. An alternative production of sclerotia is therefore imperative. This research was conducted in the Mushroom Farm of the University of Port-Harcourt, Nigeria. Fresh and Composted sawdust were used for the cultivation of Pleurotus tuber-regium to determine a better substrate medium for sclerotia production. The composted sawdust was left to decompose for two weeks while the fresh sawdust was used without decomposition. Mycelia ramification and sclerotia production were observed in the course of the research. Full mycelia ramification for composted substrate took more days (26 days from inoculation of substrate with mushroom spawn) compared with fresh sawdust (25 days from inoculation of substrate with mushroom spawn). However, there was no significant difference for mycelia ramification between the substrates. Sclerotia production took shorter days on composted substrate (47 days from inoculation of substrate with mushroom spawn)and significantly different (P≤0.05) from production on the fresh sawdust (49 days from inoculation of substrate with mushroom spawn). Fresh weight of sclerotia for the composted sawdust weighed 21.6± 5.6 grams while the fresh weight of sclerotia for the fresh sawdust weighed 15.6±3.9 grams; dry weight of sclerotia for the composted sawdust was 11.6±4.5 grams while that of the fresh sawdust was 8.2±3.0 grams. Composted sawdust is recommended for the cultivation of Pleurotus tuber-regium for production of sclerotia.Keywords: Pleurotus tuber-regium, Sclerotia sustainability, Fresh sawdust, Composted sawdust
Effect of spleen size on splenectomy outcome. A comparison of open and laparoscopic surgery.

BACKGROUND Laparoscopic splenectomy (LS) is gaining acceptance as an alternative to open splenectomy (OS). However, splenomegaly presents an obstacle to LS, and massive splenomegaly has been considered a contraindication. Analyses comparing the procedure with the open approach are lacking. The purpose of this study was to analyze the effect of spleen size on operative and immediate clinical outcome in a series of 105 LS compared with a series of 81 cases surgically treated by an open approach. METHODS Between January 1990 and November 1998, 186 patients underwent a splenectomy for a wide range of splenic disorders. Of these patients, 105 were treated by laparoscopy (group I, LS; data prospectively recorded) and 81 were treated by an open approach (group II, OS analyzed retrospectively). Patients also were classified into three groups according to spleen weight: group A, <400 g; group B, 400-1000 g; and group C, >1000 g. Age, gender, operative time, perioperative transfusion, spleen weight, conversion rate, mode of spleen retrieval (bag or accessory incision), postoperative analgesia, length of stay, and morbidity were recorded in both main groups. RESULTS Operative time was significantly longer for LS than for OS. However, LS morbidity, mortality, and postoperative stay were all lower at similar spleen weights. Spleens weighing more than 3,200 g required conversion to open surgery in all cases. When LS outcome for hematologic malignant diagnosis was compared with LS outcome for a benign diagnosis, malignancy did not increase conversion rate, morbidity, and transfusion, even though malignant spleens were larger and accessory incisions were required more frequently. Postoperative hospital stay was significantly longer in malignant than in benign diagnosis (5 +/- 2.4 days vs. 4 +/- 2.3 days; p < 0. 05). CONCLUSIONS In patients with enlarged spleens, LS is feasible and followed by lower morbidity, transfusion rate, and shorter hospital stay than when the open approach is used. For the treatment of this subset of patients, who usually present with more severe hematologic diseases related to greater morbidity, LS presents potential advantages.
Back to Butterworth - a Fourier basis for 3D surface relief hole filling within RGB-D imagery

We address the problem of hole filling in RGB-D (color and depth) images, obtained from either active or stereo based sensing, for the purposes of object removal and missing depth estimation. This is performed independently on the low frequency depth information (surface shape) and the high frequency depth detail (relief) by way of a Fourier space transform and classical Butterworth high/low pass filtering. The high frequency detail is then filled using a texture synthesis method, whilst the low frequency shape information is inpainted using structural inpainting. Here, a classical non-parametric sampling approach is extended, using the concept of query expansion, to perform high frequency depth synthesis with the final output then recombined in Fourier space. In order to improve the overall depth relief (D) and edge detail accuracy, color information (RGB) is also used to constrain the sampling process within high frequency component completion. Experimental results demonstrate the efficacy of the proposed method outperforming prior work for generalized depth filling in the presence of high frequency surface relief detail.
Are Material Weaknesses in Internal Controls Associated with Poor M&A Decisions? Evidence from Goodwill Impairment

SUMMARY This paper examines the effect of the quality of a firm's internal control over financial reporting (ICFR) on the quality of corporate M&A decisions. We use material weaknesses in internal control (ICMWs) from SOX 404 audits as a proxy for the quality of a firm's ICFR and use future goodwill impairment to proxy for the quality of managers' M&A decisions. We find that goodwill recognized from acquisitions in years concurrent with ICMWs has a greater rate of impairment in subsequent years than goodwill recognized from acquisitions in years without ICMWs, thereby establishing a link between ICMW and goodwill impairment. We further show that disclosure and remediation of ICMWs appear to improve valuations of subsequent acquisitions. Our study contributes to the literature on internal controls by documenting unanticipated benefits of SOX 404 audits on managerial performance, and to the goodwill literature by identifying ICMWs as a determinant of goodwill impairment.
Towards Understanding Generalization of Deep Learning: Perspective of Loss Landscapes

It is widely observed that deep learning models with learned parameters generalize well, even with much more model parameters than the number of training samples. We systematically investigate the underlying reasons why deep neural networks often generalize well, and reveal the difference between the minima (with the same training error) that generalize well and those they don't. We show that it is the characteristics the landscape of the loss function that explains the good generalization capability. For the landscape of loss function for deep networks, the volume of basin of attraction of good minima dominates over that of poor minima, which guarantees optimization methods with random initialization to converge to good minima. We theoretically justify our findings through analyzing 2-layer neural networks; and show that the low-complexity solutions have a small norm of Hessian matrix with respect to model parameters. For deeper networks, extensive numerical evidence helps to support our arguments.
Galanin Receptor 1 Has Anti-proliferative Effects in Oral Squamous Cell Carcinoma*

In the United States, oral cancer accounts for more deaths annually than cervical cancer, leukemias, or Hodgkin's lymphoma. Studies have shown that aberrations of chromosome 18q develop with tumor progression and are associated with significantly decreased survival in head and neck cancer patients. The G-protein-coupled receptor, galanin receptor 1 (GALR1), maps to this region of chromosome 18q. Although the role of GALR1 has been well characterized in neuronal cells, little is known regarding this receptor in non-neuronal cells. In this study, the expression, mitogenic function, and signaling mechanism of GALR1 are investigated in normal and malignant oral epithelial cells. mRNA expression was determined via reverse transcriptase-PCR. Protein quantification was done via immunoblot analysis and enzyme-linked immunosorbent assay. For functional and signaling studies, an inhibitory antibody was generated to the N-terminal ligand binding domain of GALR1. GALR1 protein and mRNA expression and GAL secretion were detected at variable levels in immortalized human oral keratinocytes and human oropharyngeal squamous cell carcinoma cell lines. Upon competitive inhibition of GALR1, proliferation was up-regulated in immortalized and malignant keratinocytes. Furthermore, studies with the inhibitory antibody and U0126, the MAPK inhibitor, show that GALR1 inhibits proliferation in immortalized and malignant keratinocytes by inactivating the MAPK pathway. GALR1s inhibitory effects on proliferation in epithelial cells raises the possibility that inactivation or disregulation of this receptor can lead to uncontrolled proliferation and neoplastic transformation.
Prognosis of ARF in hospitalized elderly patients.

BACKGROUND Differentiation between hospital-acquired acute renal failure (ARF) and community-acquired ARF may have epidemiological implications that lead to different prognoses in hospitalized patients. Such a comparison has not yet been made among elderly individuals. METHODS We performed a 3-year prospective study in a tertiary referral hospital of 325 patients aged 60 years or older who presented with ARF. Patients were divided into 2 groups; those with hospital-acquired ARF (n = 154) and community-acquired ARF (n = 171), and were followed up in relation to mortality. Multiple logistic regression was used in the analysis. RESULTS The overall mortality rate in this elderly population was 54%; 59% for the group with hospital-acquired ARF and 41% for the group with community-acquired ARF (P < 0.001). Groups differed (P < 0.01) in relation to cause of ARF, preexisting diseases, organ failure, sepsis, and performance of dialysis, among other factors. The adjusted mortality risk for the group with hospital-acquired ARF was 2.23 times greater than for the group with community-acquired ARF (95% confidence interval [CI], 1.21 to 4.08). In the group with hospital-acquired ARF, factors associated with mortality were neurological failure (odds ratio [OR], 2.97; 95% CI, 1.17 to 7.60), hematologic failure (OR, 4.30; 95% CI, 1.63 to 11.34), and oliguria (OR, 12.14; 95% CI, 4.62 to 31.87). In the group with community-acquired ARF, significant factors were neoplasia, cardiac disease, hepatic disease, cardiovascular failure, oliguria, and sepsis. CONCLUSION Differentiation between hospital-acquired ARF and community-acquired ARF is important in determining the prognosis of ARF in the elderly. Mortality risk factors are different in these 2 groups, and knowledge of their characteristics may allow better management of such patients.
Optimization of Receptor-G Protein Coupling by Bilayer Lipid Composition I

The role of membrane composition in modulating the rate of G protein-receptor complex formation was examined using rhodopsin and transducin (Gt) as a model system. Metarhodopsin II (MII) and MII-Gt complex formation rates were measured, in the absence of GTP, via flash photolysis for rhodopsin reconstituted in 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (18:0,18:1PC) and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (18:0,22:6PC) bilayers, with and without 30 mol% cholesterol. Variation in bilayer lipid composition altered the lifetime of MII-Gt formation to a greater extent than the lifetime of MII. MII-Gt formation was fastest in 18:0,22:6PC and slowest in 18:0,18:1PC/30 mol% cholesterol. At 37 °C and a Gt to photolyzed rhodopsin ratio of 1:1 in 18:0,22:6PC bilayers, MII-Gt formed with a lifetime of 0.6 ± 0.06 ms, which was not significantly different from the lifetime for MII formation. Incorporation of 30 mol% cholesterol slowed the rate of MII-Gt complex formation by about 400% in 18:0,18:1PC, but by less than 25% in 18:0,22:6PC bilayers. In 18:0,22:6PC, with or without cholesterol, MII-Gt formed rapidly after MII formed. In contrast, cholesterol in 18:0,18:1PC induced a considerable lag time in MII-Gt formation after MII formed. These results demonstrate that membrane composition is a critical factor in determining the temporal response of a G protein-coupled signaling system.
Plasma Kallikrein Mediates Angiotensin II Type 1 Receptor–Stimulated Retinal Vascular Permeability

Hypertension is a leading risk factor for the development and progression of diabetic retinopathy and contributes to a variety of other retinal diseases in the absence of diabetes mellitus. Inhibition of the renin-angiotensin system has been shown to provide beneficial effects against diabetic retinopathy, both in the absence and presence of hypertension, suggesting that angiotensin II (Ang II) and the Ang II type 1 receptor may contribute to retinal vascular dysfunction. We investigated the effects of the Ang II type 1 receptor antagonist candesartan on retinal vascular permeability (RVP) in normotensive rats with streptozotocin-induced diabetes mellitus and in rats with Ang II–induced hypertension. We showed that candesartan treatment decreased diabetes mellitus– and Ang II–stimulated RVP by 58% (P<0.05) and 79% (P<0.05), respectively, compared with untreated controls, suggesting that activation of the Ang II type 1 receptor contributes to blood-retinal barrier dysfunction. We found that plasma kallikrein levels are increased in the retina of rats with Ang II–stimulated hypertension and that intravitreal injection of either plasma kallikrein or bradykinin is sufficient to increase RVP. We showed that a novel small molecule inhibitor of plasma kallikrein, 1-benzyl-1H-pyrazole-4-carboxylic acid 4-carbamimidoyl-benzylamide, delivered systemically via a subcutaneous pump, decreased Ang II–stimulated RVP by 70% (P<0.05) and ameliorates Ang II–induced hypertension, measured from the carotid artery by telemetry, but did not reduce Ang II–induced retinal leukostasis. These findings demonstrate that activation of the Ang II type 1 receptor increases RVP and suggest that systemic plasma kallikrein inhibition may provide a new therapeutic approach for ameliorating blood-retinal barrier dysfunction induced by hypertension.
Bidirectional, High-Power DC Transformer

This paper studies a bidirectional dc-dc converter concept which is capable of achieving very high stepping ratios with MW level power transfers. The converter can find potential application in connecting high-power dc sources, interfacing to high-voltage dc transmission or to flexible ac transmission system elements. The converter is based on two resonant circuits which share a common ac capacitor. The topology is simple and utilizes thyristors with potentially all soft switchings. Complete analytical modelling is presented which enables systematic design procedure for the converter. The detailed digital simulation on PSCAD platform confirms satisfactory operation on a 5-MW test system, which connects 4-kV dc source to an 80-kV high voltage dc grid. The converter shows good responses to rapid changes in power magnitude/direction and it is concluded that robustness to terminal voltage disturbances is excellent. Since the highest-power phase-control thyristors are employed, the converter can potentially be used at much higher power levels. The passive components are of reasonable size.
FDG PET studies during treatment: Prediction of therapy outcome in head and neck squamous cell carcinoma

Positron emission tomography (PET) provides metabolic information of tissues in vivo. The purpose of this study was to assess the value of PET with 2‐[18 F] fluoro‐2‐deoxy‐D‐glucose (FDG) in prediction of therapy outcome (tumor response, survival, and locoregional control) in locally advanced HNSCC.
Cerebellar Ataxia by Enhanced CaV2.1 Currents Is Alleviated by Ca2+-Dependent K+-Channel Activators in Cacna1aS218L Mutant Mice

Mutations in the CACNA1A gene are associated with neurological disorders, such as ataxia, hemiplegic migraine, and epilepsy. These mutations affect the pore-forming α1A-subunit of CaV2.1 channels and thereby either decrease or increase neuronal Ca2+ influx. A decreased CaV2.1-mediated Ca2+ influx has been shown to reduce the regularity of cerebellar Purkinje cell activity and to induce episodic cerebellar ataxia. However, little is known about how ataxia can be caused by CACNA1A mutations that increase the Ca2+ influx, such as the S218L missense mutation. Here, we demonstrate that the S218L mutation causes a negative shift of voltage dependence of CaV2.1 channels of mouse Purkinje cells and results in lowered thresholds for somatic action potentials and dendritic Ca2+ spikes and in disrupted firing patterns. The hyperexcitability of Cacna1aS218L Purkinje cells was counteracted by application of the activators of Ca2+-dependent K+ channels, 1-EBIO and chlorzoxazone (CHZ). Moreover, 1-EBIO also alleviated the irregularity of Purkinje cell firing both in vitro and in vivo, while CHZ improved the irregularity of Purkinje cell firing in vitro as well as the motor performance of Cacna1aS218L mutant mice. The current data suggest that abnormalities in Purkinje cell firing contributes to cerebellar ataxia induced by the S218L mutation and they advocate a general therapeutic approach in that targeting Ca2+-dependent K+ channels may be beneficial for treating ataxia not only in patients suffering from a decreased Ca2+ influx, but also in those suffering from an increased Ca2+ influx in their Purkinje cells.
The psmα Locus Regulates Production of Staphylococcus aureus Alpha-Toxin during Infection

ABSTRACT Staphylococcus aureus is a leading cause of human bacterial infection, causing a wide spectrum of disease ranging from skin and soft tissue infections to life-threatening pneumonia and sepsis. S. aureus toxins play an essential role in disease pathogenesis, contributing to both immunomodulation and host tissue injury. Prominent among these toxins are the membrane-active pore-forming cytolysin alpha-toxin (Hla) and the amphipathic α-helical phenol-soluble modulin (PSM) peptides. As deletion of either the hla or psm locus leads to a phenotypically similar virulence defect in skin and soft tissue infection, we sought to determine the relative contribution of each locus to disease pathogenesis. Here we show that production of Hla can be modulated by PSM expression. An S. aureus mutant lacking PSM expression exhibits a transcriptional delay in hla mRNA production and therefore fails to secrete normal levels of Hla at early phases of growth. This leads to attenuation of virulence in vitro and in murine skin and lung models of infection, correlating with reduced recovery of Hla from host tissues. Production of Hla and restoration of staphylococcal virulence can be achieved in the psm mutant by plasmid-driven overexpression of hla. Our study suggests the coordinated action of Hla and PSMs in host tissue during early pathogenesis, confirming a major role for Hla in epithelial injury during S. aureus infection. These findings highlight the possibility that therapeutics targeting PSM production may simultaneously prevent Hla-mediated tissue injury.
The sirtuin SIRT6 regulates stress granule formation in C. elegans and mammals

Summary SIRT6 is a NAD+-dependent deacetylase that modulates chromatin structure and safeguards genomic stability. Until now, SIRT6 has been assigned to the nucleus and only nuclear targets of SIRT6 are known. Here, we demonstrate that in response to stress, C. elegans SIR-2.4 and its mammalian orthologue SIRT6 localize to cytoplasmic stress granules, interact with various stress granule components and induce their assembly. Loss of SIRT6 or inhibition of its catalytic activity in mouse embryonic fibroblasts impairs stress granule formation and delays disassembly during recovery, whereas deficiency of SIR-2.4 diminishes maintenance of P granules and decreases survival of C. elegans under stress conditions. Our findings uncover a novel, evolutionary conserved function of SIRT6 in the maintenance of stress granules in response to stress.
Transfer Literacy: Assessing Informational Symmetries and Asymmetries

This research examines the extent to which the Ontario college-to-university transfer information system is performing efficiently and identifies symmetries and asymmetries in stakeholders’ understanding of this process. Recognized symmetries were identified in areas where shared (or uniform) knowledge exists. Asymmetries were identified in areas where some stakeholder(s) would directly benefit from additional information from the other(s) that is not being fully disseminated, resulting in non-uniform knowledge. As a lens to analyze and interpret results, the study utilizes concepts from contract theory, a branch of economic research which investigates how stakeholders interact and form contractual arrangements often in the presence of asymmetric information, and behavioural economics (Martimort, 2008). A qualitative methodology was employed, which included a review of over 70 documents representative of credit transfer deliberations in the province from 1999-2012, as well as focus groups with institutional administrators involved in the advisement of students and/or the assessment of transfer credit. Overall, 13 Ontario postsecondary institutions (six colleges and seven universities) participated in this research.
Fracture of the Lateral Process of the Talus in Children: A Kind of Ankle Injury With Frequently Missed Diagnosis

Objective: To analyze the clinical characteristics, the treatment, and the outcome of lateral process fracture of the talus in children. Methods: From March 2011 to October 2013, 12 children with lateral process fracture of the talus were treated in our hospital. The study contained 3 female and 9 male patients, including 8 patients affected on the left side and 4 on the right side. The age at the time of injury ranged from 8 to 13 years. Concomitant injuries included undisplaced calcaneus fractures in 1 case and distal fibula epiphysis injury in 1 case. The patients presented to our hospital from 2 hours to 2 months after injury. All cases were classified by the Hawkins fracture system. Treatment included immobilization and not bearing weight for 4 weeks for nondisplaced fractures or open reduction and fixation for significantly displaced fractures. Therapeutic effects were evaluated on the basis of the AOFAS (The American Orthopaedic Foot and Ankle Society) Ankle Hindfoot Scale. Results: Seven of the cases were initially diagnosed in our department, and the diagnosis was missed in 5 cases. The missed diagnosis rate was 42%. All patients were followed up for 18 months on an average. Follow-up radiographs did not show avascular necrosis of the talus, nonunion, and malunion in any patient. The mean AOFAS hindfoot score was 96 points. The clinical result was found to be excellent in 10 patients, good in 1 patient, and fair in 1 patient (the success rate was 92%). Conclusions: The lateral process of talus fracture is a frequently missed injury. The diagnosis must rely on thorough check-ups combined with standard radiographs and computed tomographic scan. Depending on the type and the displacement of the fracture, proper treatment options could be implemented for desirable clinical effects.