Miracle-dz/UniFAR-FTUs huggingface dataset:
2001 College of Occupational Therapists' Research and Development Strategic Vision and Action Plan

The Research and Development Strategic Vision and Action Plan proposes a flexible approach for the next 5 years, being responsive to changes in the policy context and reflecting the needs of members, consumers and carers. Equal importance is attached both to research and to development. This strategic shift will support action to produce quality outcomes from all occupational therapy personnel, together with the production of the national research agenda by the College of Occupational Therapists.
Interstitial telomeric sequences are not preferentially involved in the chromosome damage induced by the methylating compound streptozotocin in chinese hamster cells

The effect of the methylating compound streptozotocin (STZ) on interstitial telomeric sequences (ITSs) was investigated in Chinese hamster ovary (CHO) cells by using peptide nucleic acid‐fluorescence in situ hybridization with a pantelomeric probe. Cells were exposed to increasing concentrations of STZ, and chromosomal aberrations were analyzed at the first mitosis after treatment. The frequency of chromosomal aberrations directly involving ITSs increased in STZ‐treated cells by a factor of 2.6 (2 mM) and 3.6 (4 mM) when compared with the frequency of these aberrations in control cells (P < 0.05). However, no significant differences were found between control and exposed cells in the percentage of aberrations directly involving ITSs, demonstrating that these repeat regions were not preferentially involved in the chromosome damage induced by STZ. In addition, STZ did not alter telomerase activity, suggesting that this enzyme may not be involved in the induction of chromosomal aberrations by this compound. Environ. Mol. Mutagen. , 2013. © 2012 Wiley Periodicals, Inc.
Management of uterine fibroids at the University of Ilorin Teaching Hospital: A 5 year review

Uterine fibroid is a cause of significant morbidity in women of reproductive age group and could lead to death. This was a retrospective review of the case notes of all surgically treated cases of uterine fibroids at the University of Ilorin Teaching Hospital from the 1 st of January 2006 to the 31 st of December 2011. The results show that uterine fibroids constituted 3.0% of new gynaecology admissions. More than onethird of the patients (39.6%) were within the 30-39 years age-group and 62% occurred among women with the parity of 0 and 1. The majority (42.9%) of the women were nulipara. Infertility (78.4%), menstrual disturbances (70.5%) and abdominal swelling (52%) were common presenting features. Evidence of pelvic inflammatory disease in association with uterine fibroids was demonstrated in 58.8% of the patients. Abdominal Myomectomy was the commonest method of treatment in 60.4% of patients; the choice mainly based on age, parity and patient’s desire to maintain her reproductive potential. Anaemia occurred in 40.7% of patients mainly due to intraoperative heamorrhage. Sarcomatous change in the fibroids was present in two (0.6%) of the patients. Surgical operations for uterine fibroids are common kind of gynaecological operations at the University of Ilorin Teaching Hospital. It is therefore suggested that administration of vasopressin into the myoma bed will go a long way to reduce intraoperative haemorrhage.
A critical review of diabetes, glycemic control, and dental implant therapy.

OBJECTIVES To systematically examine the evidence guiding the use of implant therapy relative to glycemic control for patients with diabetes and to consider the potential for both implant therapy to support diabetes management and hyperglycemia to compromise implant integration. MATERIAL AND METHODS A systematic approach was used to identify and review clinical investigations directly assessing implant survival or failure for patients with diabetes. A MEDLINE (PubMED) database search identified potential articles for inclusion using the search strategy: (dental implants OR oral implants) AND (diabetes OR diabetic). Inclusion in this review required longitudinal assessments including at least 10 patients, with included articles assessed relative to documentation of glycemic status for patients. RESULTS Although the initial search identified 129 publications, this was reduced to 16, for inclusion. Reported implant failure rates for diabetic patients ranged from 0% to 14.3%. The identification and reporting of glycemic control was insufficient or lacking in 13 of the 16 studies with 11 of these enrolling only patients deemed as having acceptable glycemic control, limiting interpretation of findings relative to glycemic control. Three of the 16 studies having interpretable information on glycemic control failed to demonstrate a significant relationship between glycemic control and implant failure, with failure rates ranging from 0% to 2.9%. CONCLUSIONS Clinical evidence is lacking for the association of glycemic control with implant failure while support is emerging for implant therapy in diabetes patients with appropriate accommodations for delays in implant integration based on glycemic control. The role for implants to improve oral function in diabetes management and the effects of hyperglycemia on implant integration remain to be determined.
Material attrition and bone micromorphology after conventional and ultrasonic implant site preparation.

OBJECTIVES Little is known about the recently introduced ultrasonic implant site preparation. The purpose of this study was to compare material attrition and micromorphological changes after ultrasonic and conventional implant site preparations. MATERIAL AND METHODS Implant site preparations were performed on fresh bovine ribs using one conventional (Straumann, Freiburg, Germany) and two ultrasonic (Piezosurgery; Mectron Medical Technology, Carasco, Italy and Variosurg; NSK, Tochigi, Japan) systems with sufficient saline irrigation. Sections were examined by environmental scanning electron microscopy (ESEM). Energy-dispersive X-ray spectroscopy (EDX) was performed to evaluate the metal attrition within the bone and the irrigation fluid. RESULTS ESEM After conventional osteotomy, partially destroyed trabecular structures of the cancellous bone that were loaded with debris were observed, whereas after ultrasonic implant site preparations, the anatomic structures were preserved. EDX: None of the implant site preparation methods resulted in metal deposits in the adjacent bone structures. However, within the irrigation liquid, there was significantly higher metal attrition with ultrasonic osteotomy (P < 0.0001 and P < 0.0001 for Mectron and NSK, respectively). Whereas for Straumann system used, 15.5% of the SEM/EDX findings were drill-origin metals, this percentage increased to 37.3% and 37.9% with the application of Mectron and NSK, respectively. CONCLUSIONS Ultrasonic implant site preparation is associated with the preservation of bone microarchitecture and with the increased attrition of metal particles. Therefore, copious irrigation seems to be even more essential for ultrasonic implant site preparation than for the conventional method.
Impact of cell cycle delay on micronucleus frequency in TK6 cells

Previous studies with TK6 cells have shown that extending the recovery period after pulse treatment allows for greater micronucleus expression for some compounds. This study explores the role of cell cycle delay in micronucleus expression after pulse treatment with three model genotoxins [mitomycin C, etoposide (ETOP), vinblastine]. Cells were treated for 4 hr and allowed to recover for 36 hr with samples removed at various time points during the recovery period and analyzed for cell cycle distribution, apoptosis and micronucleus frequency. Our results show that mitomycin C causes cell cycle delay for 20 hr after pulse treatment and cell cycle perturbation is no longer evident after 36 hr of recovery. The micronucleus frequency of cells sampled at 36 hr is doubled when compared with cells sampled at 20 hr after mitomycin C removal. When cells were treated with indirect acting genotoxins (ETOP, vinblastine), cell cycle perturbation was not observed at the 20 hr time point. Micronucleus frequency after treatment with either ETOP or vinblastine did not differ between the 20 hr and the 36 hr time point. All three compounds induced similar levels of apoptosis ranging from 4.5 to 5.6% with maximum induction occurring at the 36‐hr time point. We conclude that TK6 cells exhibit extended cell cycle arrest after exposure to MMC and can go on to express micronuclei, after overcoming cell cycle arrest. Environ. Mol. Mutagen. 55:64–69, 2014. © 2013 Wiley Periodicals, Inc.
The effect of diabetes on bone formation following application of the GBR principle with the use of titanium domes.

OBJECTIVE The aim of the study was to evaluate the effect of experimental diabetes and metabolic control on de novo bone formation following the GBR principle under titanium dome with a hydrophobic or hydrophilic surface. MATERIAL AND METHODS Three groups of equal number of randomly allocated Wistar strain rats were created: (a) uncontrolled, streptozotocin-induced diabetes (D); (b) insulin-controlled diabetes (CD); (c) healthy (H). Each group was then further divided into two groups according to either 7 or 42 days of healing period, which received either a hydrophobic (SLA: A) or a hydrophilic (SLActive: B) dome. The undecalcified sections were evaluated by qualitative and quantitative histological analysis and the differences between means for the groups (D, CD, and H) and the type of domes (SLA and SLActive) at each of two observational periods (i.e. 7 and 42 days) were assessed by performing a two-way analysis of variance (ANOVA). RESULTS In all experimental groups, significant de novo bone formation under the domes was observed at 42 days of healing. There was a tendency of increased new total bone (TB) formation in H and CD groups compared to D group at 42 days of healing. Also, the SLActive titanium surface showed a trend of promoting superior TB formation at the early observational period among the experimental groups, however these differences did not reach statistical significance. In regards to the bone-to-implant contact (BIC%) under the both dome treatments (SLA and SLActive), there was no statistically significant difference among the H, CD, and D groups at both 7 and 42 days. CONCLUSION Despite of the presence of uncontrolled diabetes, substantial de novo bone formation can be achieved in titanium domes with a hydrophobic and a hydrophilic surface. The use of SLActive titanium surface may present a tendency to promote new bone formation in healthy and diabetic conditions at 7 days of healing, however the obtained data do not allow any robust conclusions.
Determining Whether Or When To Adopt New Versions Of Psychological And Neuropsychological Tests: Ethical And Professional Considerations

Test selection has significant implications for inferences that can be drawn from test data. Some tests undergo revisions, typically to improve their psychometric properties, normative data, relevance of stimuli, and ease of administration. Although revisions of psychological and neuropsychological tests are published periodically, little information is available regarding whether or when clinicians should transition to the most recent versions of the tests. The 2002 APA Ethics Code (Standard 9.08b) requires that psychologists not base their assessment or intervention decisions or recommendations “on tests and measures that are obsolete and not useful for the current purpose. ” However, there is no consensus regarding when tests should no longer be considered acceptable, and there may be sound reasons for delaying or foregoing the purchase and use of new versions of assessment measures. Determining whether or when to transition to a new version of a test can be particularly difficult for clinicians in psychological specialties because it can take years after publication of a revised test for research with special patient populations to be performed and published. As a result, different clinicians may adopt newer versions of tests at different times or elect not to use the newest version, depending on the specific patient population and referral questions. Decisions regarding transitioning to new test revisions should be based on the scientific merits of the tests, not on an arbitrarily defined time frame. Clinicians ultimately must use their judgment regarding which test version is best for a given patient at a given point in time.
A cross-sectional study on the prevalence of marginal bone loss among implant patients.

OBJECTIVE The aim of the present cross-sectional retrospective study was to determine bone loss in a sample of subjects restored with implant-supported prostheses and the prevalence and severity of peri-implantitis in a sub-sample. MATERIAL AND METHODS A total of 139 patients who had attended a follow-up visit in 2007 were considered for inclusion. Subjects with implants that had been in function for less than 3 years or had poor quality radiographs were excluded. The final study population comprised 133 subjects with a total of 407 implants. Radiographic measurements identified subjects who had ≥1 implant site exhibiting marginal bone loss of >0.5 mm; 40 subjects met this criterion and were recalled for a clinical examination. Of the 40 subjects that were recalled for the clinical examination, 30 attended. The following parameters were recorded at mesial, distal, buccal, and lingual/palatal aspects of all implants: oral hygiene standard (plaque), bleeding on probing, probing pocket depth (PPD). RESULTS The mean interval between the baseline (1-year post-loading) and the follow-up radiographs was 4.8 ± 2.3 years. In the total subject sample (133 subjects and 407 implants), the mean amount of marginal bone loss that had occurred was 0.2 ± 1.2 mm. Ninety-three subjects with 246 implant sites exhibited no bone level alteration (group A), whereas 40 subjects with 161 implant sites (group B) displayed marginal bone loss of >0.5 mm at ≥1 implant (loser site). Sixty-eight implant sites in group B exhibited bone loss of >0.5 mm. However, only 20% of subjects and 11% of sites had lost >1 mm marginal bone, and 8% of subjects and 4% of sites had lost >2 mm bone. The total amount of bone loss that had occurred in group B was (i) 0.88 ± 1.5 mm and (ii) among the loser sites 2.1 ± 1.4 mm. Thirty subjects from group B were exposed to a clinical examination; out of 37 sites with bone loss >0.5 mm in this subgroup, 29 sites had a PPD value of ≥4 mm. CONCLUSION Marginal bone loss (>0.5 mm) at implants was observed in 30% of subjects and 16% of implant sites. More advanced loss of marginal bone occurred in much fewer subjects and sites. Sites with marginal bone loss was in the sub-sample characterized by bleeding on probing, but only occasionally with deep (≥6 mm) pockets.
A comparison of cone beam computed tomography and conventional periapical radiography at detecting peri-implant bone defects.

OBJECTIVE To compare the diagnostic accuracy of conventional periapical radiography and cone beam computed tomography (CBCT) at detecting peri-implant bone defects. MATERIALS AND METHODS Implants were placed in fresh bovine ribs in osteotomy sites of varying diameter (five with no peri-implant space, five with a 0.35 mm space, five with a 0.675 mm space) and imaged using (i) digital long cone periapical radiographs (LCPAs), (ii) limited volume CBCT using 3D Accuitomo 80(®) and (iii) large volume CBCT using i-CAT Next Generation(®). Images from each were randomly presented to nine examiners on two occasions. Confidence in diagnosing the presence or absence of a peri-implant radiolucency was recorded on a five-point scale. Receiver Operating Characteristic (ROC) analysis and Kappa tests were performed. RESULTS Digital LCPAs were better at diagnosing a peri-implant bone defect when the peri-implant space was 0.35 mm (P < 0.02). As the peri-implant space increased to 0.675 mm, there was no significant difference in diagnostic accuracy between the three imaging methods. Sensitivity of LCPAs (100) and Accuitomo (97.8) was better than i-CAT (64.4) (P < 0.02). LCPAs and i-CAT had significantly better specificity and positive predictive value than Accuitomo. The negative predictive value of LCPA was significantly better than i-CAT. LCPAs showed better intra-examiner and inter-examiner agreement than CBCT. CONCLUSION Within the limitations of this study, LCPAs are a reliable and valid method of detecting circumferential peri-implant bone defects and performed significantly better than CBCT.
Micronucleus formation in human keratinocytes is dependent on radiation quality and tissue architecture

The cytokinesis‐block micronucleus (MN) assay was used to assess the genotoxicity of low doses of different types of space radiation. Normal human primary keratinocytes and immortalized keratinocytes grown in 2D monolayers each were exposed to graded doses of 0.3 or 1.0 GeV/n silicon ions or similar energies of iron ions. The frequencies of induced MN were determined and compared to γ‐ray data. RBEmax values ranged from 1.6 to 3.9 for primary keratinocytes and from 2.4 to 6.3 for immortalized keratinocytes. At low radiation doses ≤0.4 Gy, 0.3 GeV/n iron ions were the most effective at inducing MN in normal keratinocytes. An “over‐kill effect” was observed for 0.3 GeV/n iron ions at higher doses, wherein 1.0 GeV/n iron ions were most efficient in inducing MN. In immortalized keratinocytes, 0.3 GeV/n iron ions produced MN with greater frequency than 1.0 GeV/n iron ions, except at the highest dose tested. MN formation was higher in immortalized keratinocytes than in normal keratinocytes for all doses and radiation qualities investigated. MN induction was also assessed in human keratinocytes cultured in 3D to simulate the complex architecture of human skin. RBE values for MN formation in 3D were reduced for normal keratinocytes exposed to iron ions, but were elevated for immortalized keratinocytes. Overall, MN induction was significantly lower in keratinocytes cultured in 3D than in 2D. Together, the results suggest that tissue architecture and immortalization status modulate the genotoxic response to space radiation, perhaps via alterations in DNA repair fidelity. Environ. Mol. Mutagen. 56:22–31, 2015. © 2014 Wiley Periodicals, Inc.
PPARγ delivered by Ch-GNPs onto titanium surfaces inhibits implant-induced inflammation and induces bone mineralization of MC-3T3E1 osteoblast-like cells.

OBJECTIVES To deliver the efficacy and safety of Ch-GNPs (Chitosan gold nanoparticles) conjugated anti-inflammatory molecules peroxisome proliferator activated receptor gamma (PPARγ) on implant surface titanium (Ti) to reduce implant-induced inflammation. MATERIALS AND METHODS The Ch-GNPs were conjugated with the PPARγ cDNA through a coacervation process. Conjugation was cast over Ti surfaces by dipping, and cells were seeded on different sizes (6 × 6 × 0.1 cm and 1 × 1 × 0.1 cm; n = 3) of Ti surfaces. The size of Ch-GNPs and surface characterization of Ti was performed using UV-vis spectroscopy, TEM (Transmission electron microscopy) and EDX (energy-dispersive X-ray). The DNA conjugation and transfection capacity of Ch-GNPs were simultaneously confirmed by agarose gel electrophoresis, β-galactosidase staining, and immunoblotting. RESULTS The Ch-GNPs were well dispersed and spherical in shape, with average size around 10-20 nm. Ti surfaces coated with Ch-GNPs/LacZ, as transfection efficacy molecule, showed strong β-galactosidase staining in MC-3T3 E1 cells. Cells cultured on Ch-GNPs/PPARγ-coated Ti surfaces were able to inhibit implant-induced inflammation by simultaneously suppressing the expression of tumor necrosis factor- alpha (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and matrix metalloproteinase-2 (MMP-2). The inhibition mechanism of Ch-GNPs/PPARγ was due to inhibition of both reactive oxygen species (ROS) and nitric oxide (NO) secretion (n = 3; P < 0.05). In addition, Ch-GNPs/PPARγ was able to increase expression of bone morphogenetic protein (BMP-7) and runt-related transcription factor-2 (RUNX-2). Furthermore, alkaline phosphatase activity (ALP) was also increased than that in control (n = 3; P < 0.01). Whereas, expression of receptor activator of NF-κB ligand (RANKL) was decreased. CONCLUSIONS The novel gene delivery materials, like Ch-GNPs, can carry the PPARγ cDNA into the required areas of the implant surfaces, thus aiding to inhibit inflammation and promote osteoblast function. Thus, the PPARγ on implant surfaces may promote its clinical application on peri-implantitis or periodontitis like diseases.
TiO2 nanoparticles induce DNA double strand breaks and cell cycle arrest in human alveolar cells

TiO2 nanoparticles (NPs) have the second highest global annual production (∼3000 tons) among the metal‐containing NPs. These NPs are used as photocatalysts for bacterial disinfection, and in various other consumer products including sunscreen, food packaging, therapeutics, biosensors, surface cleaning agents, and others. Humans are exposed to these NPs during synthesis (laboratory), manufacture (industry), and use (consumer products, devices, medicines, etc.), as well as through environmental exposures (disposal). Hence, there is great concern regarding the health effects caused by exposure to NPs and, in particular, to TiO2 NPs. In the present study, the genotoxic potential of TiO2 NPs in A549 cells was examined, focusing on their potential to induce ROS, different types of DNA damage, and cell cycle arrest. We show that TiO2 NPs can induce DNA damage and a corresponding increase in micronucleus frequency, as evident from the comet and cytokinesis‐block micronucleus assays. We demonstrate that DNA damage may be attributed to increased oxidative stress and ROS generation. Furthermore, genomic and proteomic analyses showed increased expression of ATM, P53, and CdC‐2 and decreased expression of ATR, H2AX, and Cyclin B1 in A549 cells, suggesting induction of DNA double strand breaks. The occurrence of double strand breaks was correlated with cell cycle arrest in G2/M phase. Overall, the results indicate the potential for genotoxicity following exposure to these TiO2 NPs, suggesting that use should be carefully monitored. Environ. Mol. Mutagen. 56:204–217, 2015. © 2014 Wiley Periodicals, Inc.
Review of Five Instruments for the Assessment of Asperger's Disorder in Adults

Due to the recent inclusion of Asperger's Disorder (AD) in the Diagnostic and Statistical Manual of Mental Disorders (APA, 1994) and the International Classification of Diseases (WHO, 1993), concerns regarding diagnosis of AD, particularly in adults, are emerging. Many existing instruments used to assess Pervasive Developmental Disorder are more appropriate for identifying Autistic Disorder (AU) in children, and their usefulness for assessing AD in adults is questionable. We describe and critically review five instruments created specifically for identifying AD in adults. Overall, the normative information provided is limited and evidence of the reliability and validity for each instrument is relatively poor. Further research and development is required before we would recommend one instrument over another for the assessment of AD in adults.