Miracle-dz/UniFAR-FTUs huggingface dataset:
Perception: a concept analysis.

PURPOSE Concept analysis methodology by Walker and Avant (2005) was used to define, describe, and delimit the concept of perception. DATA SOURCE Nursing literature in the Medline database was searched for definitions of "perception. " DATA SYNTHESIS Definitions, uses, and defining attributes of perception were identified; model and contrary cases were developed; and antecedents, consequences, and empirical referents were determined. CONCLUSIONS An operational definition for the concept was developed. IMPLICATIONS FOR NURSING PRACTICE Nurses need to be cognizant of how perceptual differences impact the delivery of nursing care. In research, a mixed methodology approach may yield a richer description of the phenomenon and provide useful information for clinical practice.
Carotenoid-binding Sites of the Major Light-harvesting Complex II of Higher Plants*

Recombinant light-harvesting complex II (LHCII) proteins with modified carotenoid composition have been obtained byin vitro reconstitution of the Lhcb1 protein overexpressed in bacteria. The monomeric protein possesses three xanthophyll-binding sites. The L1 and L2 sites, localized by electron crystallography in the helix A/helix B cross, have the highest affinity for lutein, but also bind violaxanthin and zeaxanthin with lower affinity. The latter xanthophyll causes disruption of excitation energy transfer. The occupancy of at least one of these sites, probably L1, is essential for protein folding. Neoxanthin is bound to a distinct site (N1) that is highly selective for this species and whose occupancy is not essential for protein folding. Whereas xanthophylls in the L1 and L2 sites interact mainly with chlorophyll a, neoxanthin shows strong interaction with chlorophyll b, inducing the hyperchromic effect of the 652 nm absorption band. This observation explains the recent results of energy transfer from carotenoids to chlorophyllb obtained by femtosecond absorption spectroscopy. Whereas xanthophylls in the L1 and L2 sites are active in photoprotection through chlorophyll-triplet quenching, neoxanthin seems to act mainly in 1O2 *scavenging.
Health Sciences Librarians' Research on Medical Students' Use of Information for Their Studies at The Medical School, University of Queensland, Australia

This study reports the findings of research undertaken by health sciences librarians at the University of Queensland Library into how medical students use information for their studies, particularly resources and services provided by the Library. The methods utilized were an online survey and focus groups. Results indicated that students favor print resources over electronic, value accessing resources on a one-stop basis, and prefer training to be delivered flexibly. The implication of these results for future resource selection, service provision, and instructional design and delivery is discussed.
Secretion and transport of mouse epididymal proteins after injection of 35S-methionine.

The presence of epididymal secretory proteins in crude luminal fluids of the epididymis of mice was investigated at varying times after i.v. injection of 35S-methionine or after incubation of epididymal minces with 35S-methionine. The amount of label incorporated into luminal proteins after in vivo injection was not significantly different at 4, 8, 12, and 16 h. The quantity of labeled proteins in the crude luminal fluids of Regions 1-3 (caput) was about two and four times higher than in Regions 4 (corpus) and 5 (cauda), respectively. Increasing the dosage of 35S-methionine strongly increased the amount of labeled protein present. Approximately half of the labeled protein present in the epididymis was found in the luminal fluid. Polyacrylamide gel electrophoresis revealed comparable patterns of proteins at 8 h after injection of isotope or after a 5-h in vitro incubation of minced epididymal tissues with isotope. The protein patterns from the five regions, however, were markedly different from each other and highly characteristic. Two proteins (25 kDa and 18 kDa) were found in crude luminal fluids of Regions 2 and 3 eight hours after in vivo injection, but not in Regions 4 and 5. One protein (29 kDa) was found in high amounts in Regions 4 and 5 eight hours after injection. Five days after injection, the three proteins were found in Regions 4 and 5. However, the 25-kDa protein was present in reduced amount, whereas the 18- and 29-kDa proteins accumulated in caudal fluid. (ABSTRACT TRUNCATED AT 250 WORDS)
Repeat motifs of tau bind to the insides of microtubules in the absence of taxol

The tau family of microtubule‐associated proteins has a microtubule‐binding domain which includes three or four conserved sequence repeats. Pelleting assays show that when tubulin and tau are co‐ assembled into microtubules, the presence of taxol reduces the amount of tau incorporated. In the absence of taxol, strong binding sites for tau are filled by one repeat motif per tubulin dimer; additional tau molecules bind more weakly. We have labelled a repeat motif with nanogold and used three‐dimensional electron cryomicroscopy to compare images of microtubules assembled with labelled or unlabelled tau. With kinesin motor domains bound to the microtubule outer surface to distinguish between α‐ and β‐tubulin, we show that the gold label lies on the inner surface close to the taxol binding site on β‐tubulin. Loops within the repeat motifs of tau have sequence similarity to an extended loop which occupies a site in α‐tubulin equivalent to the taxol‐binding pocket in β‐tubulin. We propose that loops in bound tau stabilize microtubules in a similar way to taxol, although with lower affinity so that assembly is reversible.
[Short-term efficacy comparison between Ivor-Lewis approach and McKeown approach in minimally invasive esophagectomy].

OBJECTIVE To compare the perioperative complications between Ivor-Lewis approach and McKeown approach in minimally invasive esophagectomy and gastric tube reconstruction for the treatment of middle and lower thoracic esophageal cancer. METHODS Retrospective analysis of clinical data was performed on 288 patients with middle and lower thoracic esophageal cancer who underwent completely minimally invasive esophagectomy by one surgical team in Fujian Medical University Union Hospital from December 2010 to March 2014. Among the 288 patients, 103 patients underwent combined laparoscopic and thoracoscopic esophagectomy and intrathoracic esophagogastric anastomosis using a transoral anvil(Orvil)(Ivor-Lewis group, 2-incision) and 185 patients underwent combined laparoscopic and thoracoscopic esophagectomy and cervical anastomosis(McKeown group, 3-incision). Patients were stratified by surgical approach and perioperative outcomes were compared between the two groups. RESULTS There were no statistical differences between two groups in intra-operative blood loss, conversion to open, extubation time, time to resume oral intake, postoperative hospital stay, the median number of lymph nodes resected. The operation time of Ivor-Lewis group was significantly shorter than that of McKeown group [(283.4±32.0) min vs. (303.6±43.7) min, P=0.003). The hospital cost of Ivor-Lewis group was significantly higher than that of McKeown group [(76 492±18 553) yuan vs. (68 923±17 331) yuan, P<0.01]. There were no statistical differences between two groups in chylothorax, delayed gastric emptying, atrial fibrillation, postoperative bleeding, admission to ICU, short-term postoperative mortality (P>0.05). The total postoperative complication morbidity of Ivor-Lewis group was significantly lower than that of McKeown group(16.5% vs. 31.4%, P<0.01). Ivor-Lewis group had lower pulmonary complication(8.7% vs. 25.9%, P<0.01), anastomotic leakage(1.9% vs. 13.0%, P<0.01), anastomotic stricture (0% vs. 4.9%, P<0.05), recurrent laryngeal nerve injury(1.0% vs. 7.0%, P<0.05). CONCLUSION Ivor-Lewis approach is associated with less postoperative complications, but higher cost as compared to McKeown approach in the treatment of middle and lower thoracic esophageal cancer.
Transgene-mediated cosuppression and RNA interference enhance germ-line apoptosis in Caenorhabditis elegans

Introduction of multiple copies of a germ-line–expressed gene elicits silencing of the corresponding endogenous gene during Caenorhabditis elegans oogenesis; this process is referred to as germ-line cosuppression. Transformed plasmids assemble into extrachromosomal arrays resembling extra minichromosomes with repetitive structures. Loss of the transgene extrachromosomal array leads to reversion of the silencing phenomenon. Cosuppression and RNAi depend upon some of the same genes. In the C. elegans germ line, about half the cells undergo a physiological programmed cell death that shares most genetic requirements with somatic apoptosis. In addition, apoptosis is stimulated by DNA damage and synaptic failure mediated through different apoptotic checkpoints. We found that both germ-line cosuppression and RNAi of germ-line–expressed genes enhance apoptosis during C. elegans oogenesis. In contrast, apoptosis is not enhanced by extrachromosomal arrays carrying genes not driven by germ-line–specific promoters that thus do not elicit transgene-mediated cosuppression/silencing. Similarly, introduction of doubled-stranded RNA that shares no homology with endogenous genes has no effect on apoptosis. “Silencing-induced apoptosis” is dependent upon sir-2.1 and cep-1 (the worm p53 ortholog), and is accompanied by a rise in RAD-51 foci, a marker for ongoing DNA repair, indicating induction of DNA double-strand breaks. This finding suggests that the DNA damage-response pathway is involved. RNAi and cosuppression have been postulated as defense mechanisms against genomic intruders. We speculate that the mechanism here described may trigger the elimination of germ cells that have undergone viral infection or transposon activation.
Emotional and physical health of informal caregivers of residents at the end of life: the role of social support.

OBJECTIVES The objectives of this study were to examine the relationship between selected decedent and caregiver characteristics, facility-related perceptions, and emotional and physical health of 434 informal caregivers (94% family) of recently deceased residents of residential care/assisted living facilities and nursing homes. We also examined potential mediating effects of social support (informal, staff, and spiritual). METHODS We analyzed data using linear mixed models. RESULTS Among caregivers, younger age, female gender, more education, financial burden, other dependents, poorer perceptions of care, and more care involvement were associated with more emotional strain. More staff support also was associated with more emotional strain and partially mediated the relationship between having a trusted staff member and emotional strain. Characteristics associated with poorer physical health included unemployment, financial burden, poorer physician communication, and trusted staff member. Informal social support was directly related to better physical health and mediated the relationships between physical health and both physician communication and financial burden. DISCUSSION Many characteristics related to end-of-life caregiving outcomes in long-term care are consistent with community-based studies, suggesting that end-of-life caregiving outcomes in long-term care are not markedly different than in other settings. However, the role of staff support may either facilitate or complicate emotional strain and merits additional study.
Low Osteoporosis Treatment Initiation Rate in Women after Distal Forearm or Proximal Humerus Fracture: A Healthcare Database Nested Cohort Study

Treatment initiation rates following fragility fractures have often been reported to be low and in recent years numerous programs have been implemented worldwide to increase them. This study aimed at describing osteoporosis (OP) treatment initiation in a representative sample of women who were hospitalized for a distal forearm fracture (DFF) or proximal humerus fracture (PHF) in 2009–2011 in France. The data source was a nationwide sample of 600,000 individuals, extracted from the French National Insurance Healthcare System database. All women aged 50 years and older who were hospitalized for a DFF or PHF between 2009 and 2011 and who had not received any OP treatment in the preceding 12 months were included in a retrospective cohort study. OP treatments initiated during the year following the fracture were analyzed. From 2009 to 2011, 729 women were hospitalized for a DFF or a PHF and 284 were on OP treatment at the time of the fracture occurrence. Among the 445 women who had no prevalent OP treatment, 131 (29.4%) received supplementation treatment only (vitamin D and/or calcium) and 42 (9.4%) received a pharmacologic OP treatment in the year following their fracture. Pharmacological OP treatments included bisphosphonates (n = 21), strontium ranelate (n = 14), hormone replacement therapy (n = 4), or raloxifene (n = 3). General practitioners prescribed 75% of initial OP treatments. Despite the guidelines published in 2006 and the numerous initiatives to promote post-fracture OP treatment, OP treatment initiation rate in women who were hospitalized for a fragility fracture remained low in 2009–2011 in France.
Molecular mimicry and autoimmunity.

Autoimmune disease is the consequence of an immune response against self-antigens that results in the damage and eventual dysfunction of target organs. Although the triggering event in most autoimmune diseases is unknown, an infectious cause has long been postulated to explain the development of autoimmunity. Molecular mimicry is one mechanism by which infectious agents (or other exogenous substances) may trigger an immune response against autoantigens. According to this hypothesis a susceptible host acquires an infection with an agent that has antigens that are immunologically similar to the host antigens but differ sufficiently to induce an immune response when presented to .
The Infectivity of prM-Containing Partially Mature West Nile Virus Does Not Require the Activity of Cellular Furin-Like Proteases

ABSTRACT Cleavage of the flavivirus prM protein by a cellular furin-like protease is a hallmark of virion maturation. While this cleavage is a required step in the viral life cycle, it can be inefficient. Virions that retain uncleaved prM may be infectious. We investigated whether cleavage by furin of prM on partially mature West Nile virus (WNV) during virus entry contributes to infectivity. Using quantitative assays of WNV infection, we found that virions incorporating considerable amounts of uncleaved prM protein were insensitive to treatment of cells with a potent inhibitor of furin activity. Thus, partially mature WNV does not require furin-like proteases for infectivity.
Light adaptation in the primate retina: Analysis of changes in gain and dynamics of monkey retinal ganglion cells

Abstract The responses of monkey retinal ganglion cells to sinusoidal stimuli of various temporal frequencies were measured and analyzed at a number of mean light levels. Temporal modulation tuning functions (TMTFs) were measured at each mean level by varying the drift rate of a sine-wave grating of fixed spatial frequency and contrast. The changes seen in ganglion cell temporal responses with changes in adaptation state were similar to those observed in human subjects and in turtle horizontal cells and cones tested with sinusoidally flickering stimuli; “Weber's Law” behavior was seen at low temporal frequencies but not at higher temporal frequencies. Temporal responses were analyzed in two ways: (1) at each light level, the TMTFs were fit by a model consisting of a cascade of low- and high-pass filters; (2) the family of TMTFs collected over a range of light levels for a given cell was fit by a linear negative feedback model in which the gain of the feedback was proportional to the mean light level. Analysis (1) revealed that the temporal responses of one class of monkey ganglion cells (M cells) were more phasic at both photopic and mesopic light levels than the responses of P ganglion cells. In analysis (2), the linear negative feedback model accounted reasonably well for changes in gain and dynamics seen in three P cells and one M cell. From the feedback model, it was possible to estimate the light level at which the dark-adapted gain of the cone pathways in the primate retina fell by a factor of two. This value was two to three orders of magnitude lower than the value estimated from recordings of isolated monkey cones. Thus, while a model which includes a single stage of negative feedback can account for the changes in gain and dynamics associated with light adaptation in the photopic and mesopic ranges of vision, the underlying physical mechanisms are unknown and may involve elements in the primate retina other than the cone.
Three-dimensional finite-element simulation of a piezoelectric vibrator under gyration

A three-dimensional finite element modeling is applied to the characteristic evaluation of the piezoelectric gyroscopes. The Coriolis' effect is incorporated with the three-dimensional finite element code that we have already developed. There is no limitation for the vibrational modes and the direction of the gyratory axis. The piezoelectric thin plate gyroscope in plane vibration is again considered for the demonstration. The solution characteristics are compared with those of the two-dimensional modeling and also with the experimental results. Reasonable agreement is achieved. The application is then extended to a bimorph-type gyroscope vibrating out of plane, which is only numerically considered for another example of demonstration. The examination shows that, with this configuration, not only the rotation but also the gyratory axis can be detected in the three-dimensional space.
Hinge‐mediated dimerization of SMC protein is essential for its dynamic interaction with DNA

Structural maintenance of chromosomes (SMC) proteins play central roles in regulating higher order chromosome dynamics from bacteria to humans. As judged by electron microscopy, the SMC homodimer from Bacillus subtilis (BsSMC) is composed of two antiparallel, coiled‐coil arms with a flexible hinge. Site‐directed cross‐linking experiments show here that dimerization of BsSMC is mediated by a hinge–hinge interaction between self‐folded monomers. This architecture is conserved in the eukaryotic SMC2–SMC4 heterodimer. Analysis of different deletion mutants of BsSMC unexpectedly reveals that the major DNA‐binding activity does not reside in the catalytic ATPase domains located at the ends of a dimer. Instead, point mutations in the hinge domain that disturb dimerization of BsSMC drastically reduce its ability to interact with DNA. Proper hinge function is essential for BsSMC to recognize distinct DNA topology, and mutant proteins with altered hinge angles cross‐link double‐stranded DNA in a nucleotide‐dependent manner. We propose that the hinge domain of SMC proteins is not a simple dimerization site, but rather it acts as an essential determinant of dynamic SMC–DNA interactions.
Cutting Edge: CTLA-4–B7 Interaction Suppresses Th17 Cell Differentiation

Th cells that produce IL-17 (Th17 cells) are a distinct subset of Th cells implicated in several autoimmune diseases. Although CD28–B7 interaction has been shown to be involved in Th17 differentiation in vitro, the role of CTLA-4 in controlling Th17 development is completely unknown. We report in this paper that blocking the CTLA-4–B7 interaction potentiates Th17 cell differentiation in vitro and in vivo. Furthermore, blocking CTLA-4–B7 interaction in vivo confers the susceptibility of experimental autoimmune myocarditis to CD28−/− mice or increases the severity of experimental autoimmune myocarditis in wild-type mice. The enhanced disease susceptibility is mediated by heightened Th17 responses. With these results, we are the first to demonstrate that CTLA-4–B7 interaction inhibits Th17 differentiation in vitro and in vivo and suppresses Th17-mediated autoimmunity.
Using Super-Pixels and Human Probability Map for Automatic Human Subject Segmentation

Human body segmentation has many applications in a wide variety of image processing tasks, from intelligent vehicles to entertainment. A substantial amount of research has been done in the field of segmentation and it is still one of the active research areas, resulting in introduction of many innovative methods in literature. Still, until today, a method that can overcome the human segmentation problems and adapt itself to different kinds of situations, has not been introduced. Many of methods today try to use the graph-cut framework to solve the segmentation problem. Although powerful, these methods rely on a distance penalty term (intensity difference or RGB color distance). This term does not always lead to a good separation between two regions. For example, if two regions are close in color, even if they belong to two different objects, they will be grouped together, which is not acceptable. Also, if one object has multiple parts with different colors, e.g. humans wear various clothes with different colors and patterns, each part will be segmented separately. Although this can be overcome by multiple inputs from user, the inherent problem would not be solved. In this paper, we have considered solving the problem by making use of a human probability map, super-pixels and Grab-cut framework. Using this map relives us from the need for matching the model to the actual body, thus helps to improve the segmentation accuracy. As a result, not only the accuracy has improved, it also became comparable to the state-of-the-art interactive methods. key words: human segmentation, Grab-cut, super-pixel, human probability map, texture map
Variations in hospitalization rates among nursing home residents: the role of discretionary hospitalizations.

OBJECTIVE To examine variations in hospitalization rates among nursing home residents associated with discretionary hospitalization practices. DATA SOURCES Quarterly Medicaid case-mix reimbursement data from the state of Massachusetts served as the core data source for this study, which was linked with data from the Medicare Provider Analysis and Review file (MEDPAR) to specify hospitalization status, nursing facility attribute data from the state of Massachusetts to specify facility-level organizational and structural attributes, and data from the Area Resource File (ARF) to specify area market-level attributes. Data spans three years (1991-1993) to produce a longitudinal analytical file containing 72,319 person-quarter-level observations. STUDY DESIGN Two-step, multivariate logistic regression models were estimated for highly discretionary hospitalizations versus those containing less discretion, and low discretionary hospitalizations versus those containing greater amounts of physician discretion. PRINCIPAL FINDINGS Findings indicate that facility case-mix levels and area hospital bed supply levels contribute to variations in hospitalization rates among nursing home residents. Highly discretionary hospitalizations appear to be most sensitive to patient diagnoses best described as chronic, ambulatory care sensitive conditions. CONCLUSIONS Findings suggest that defining hospitalizations simply in terms of whether an event occurs versus otherwise may obscure valuable information regarding the contribution of various risk factors to highly discretionary versus low discretionary hospitalization rates.