Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of novel cysteine proteases from germinating cotyledons of soybean [Glycine max (L.) Merrill].

The enzymatic properties of novel cysteine proteases D3-alpha and beta which were purified from germinating soybean cotyledons were investigated. The enzyme activities were exhibited in the presence of a thiol reagent, such as 2-mercaptoethanol, and apparently inhibited by E-64, a cysteine protease inhibitor. Hydrolytic activities toward carbobenzoxy-Phe-Arg-MCA were detected at a pH above 4.0. The optimum temperature for activities was about 40 degrees C. The isoelectric point of D3-alpha and beta was 4.4 and 4. 7, respectively. The molecular mass of D3-alpha and beta, measured by MALDI/TOF mass spectrometry, was 26,178 and 26,429 Da, respectively. The substrate specificities of the enzymes were examined using peptide-MCAs and peptides, and cathepsin L-like broad specificity was observed at pH 4.0. These results demonstrated that these enzymes are cysteine endopeptidases [EC 3.4. 22. -] like papain [EC 3.4. 22.2].
Rapid screening and treatment for sexually transmitted diseases in arrestees: a feasible control measure.

OBJECTIVES The purpose of this study was to determine the feasibility and usefulness of rapid screening and immediate presumptive treatment for sexually transmitted diseases among arrestees at a county jail. METHODS The rapid plasma reagin test (for syphilis) and the urine leukocyte esterase test (for urethritis) were used in establishing routine screening and presumptive treatment for sexually transmitted diseases among arrestees. RESULTS Of 4757 arrestees screened, 82 (1.7%) had untreated syphilis, and, of 4174 male arrestees screened, 541 (13%) had a positive leukocyte esterase test. The syphilis prevalence rate was higher among women than among men (3.1% vs 1.5%), and the rate was higher among Blacks than among Whites (2.3% vs 0.7%). Although leukocyte esterase test positivity was higher among persons reporting a urethral discharge (57% vs 12%), 88% of persons with a positive test result did not report such symptoms. CONCLUSIONS The program described here has reached persons with sexually transmitted diseases who are unlikely to use traditional health care services and thus are likely to remain untreated. Rapid screening and treatment for sexually transmitted diseases among arrestees is feasible and should be considered in areas with high sexually transmitted disease rates.
Hydrolysis of soluble, linear, un-cross-linked peptidoglycans by endogenous bacterial N-acetylmuramoylhydrolases

Soluble, linear, uncross-linked peptidoglycans, prepared from two autolysis-defective mutants of Streptococcus faecium ATCC 9790 and from Micrococcus leuteus, were used as substrates for studies of hydrolysis by an N-acetylmuramoylhydrolase (muramidase). The kinetics of hydrolysis of these substrates and the ability of the muramidases isolated from S. faecium ATCC 9790 and from two autolysis-defective mutants, Lyt-14 and Aut-3, to carry out transglycosylation reactions were compared with the action of hen egg white lysozyme (EC 3.2.1.17). Hydrolysis of these substrates by the endogenous streptococcal muramidases resulted in the production of disaccharide-peptide monomers with the structure (formula; see text) as nearly the sole product. As estimated from increases in reducing groups, hydrolysis proceeded at a linear rate for extended intervals, with consumption of up to 75% of the substrate, even at substrate concentrations well below the Km value. Apparent Km and relative Vmax values for the three streptococcal enzymes were indistinguishable from each other or from those for hen egg white lysozyme. These results indicate that the autolysis-defective phenotype of these mutants cannot be attributed to differences in their muramidases. In contrast to the action of hen egg white lysozyme, the streptococcal muramidase failed to catalyze transglycosylations. The extended periods of hydrolysis at constant rates are consistent with the occurrence of multiple catalytic events after the formation of the enzyme-substrate complex.
Chinese Import Competition, Crime, and Government Transfers in US

This paper exploits the exogenous rise of Chinese imports in US to investigate the effect of import competition on crime at the county level. The results indicate that counties with high exposure to Chinese import competition have high crime rates. The exposure effect on property crime is much larger than that on violent crime. A one standard deviation increase in exposure causes 32 more violent crimes in the county, while such increase in exposure causes 256 more property crimes. Interestingly, we find that the crime impact of exposure to Chinese import competition becomes smaller in counties with high government transfers.
Applying Recovery Biomarkers to Calibrate Self-Report Measures of Energy and Protein in the Hispanic Community Health Study/Study of Latinos

We investigated measurement error in the self-reported diets of US Hispanics/Latinos, who are prone to obesity and related comorbidities, by background (Central American, Cuban, Dominican, Mexican, Puerto Rican, and South American) in 2010–2012. In 477 participants aged 18–74 years, doubly labeled water and urinary nitrogen were used as objective recovery biomarkers of energy and protein intakes. Self-report was captured from two 24-hour dietary recalls. All measures were repeated in a subsample of 98 individuals. We examined the bias of dietary recalls and their associations with participant characteristics using generalized estimating equations. Energy intake was underestimated by 25.3% (men, 21.8%; women, 27.3%), and protein intake was underestimated by 18.5% (men, 14.7%; women, 20.7%). Protein density was overestimated by 10.7% (men, 11.3%; women, 10.1%). Higher body mass index and Hispanic/Latino background were associated with underestimation of energy (P < 0.05). For protein intake, higher body mass index, older age, nonsmoking, Spanish speaking, and Hispanic/Latino background were associated with underestimation (P < 0.05). Systematic underreporting of energy and protein intakes and overreporting of protein density were found to vary significantly by Hispanic/Latino background. We developed calibration equations that correct for subject-specific error in reporting that can be used to reduce bias in diet-disease association studies.
Everolimus: A Proliferation Signal Inhibitor with Clinical Applications in Organ Transplantation, Oncology, and Cardiology

Everolimus, a proliferation signal inhibitor in the mammalian target of rapamycin (mTOR) drug class, has many clinical applications, including in organ transplantation, oncology, and cardiology. It currently has United States Food and Drug Administration (FDA) approval for prophylaxis against rejection in de novo renal transplant recipients, treatment of renal cell carcinoma, and use as a drug‐eluting stent. To review the pharmacology, pharmacokinetics, efficacy, and safety of everolimus, we performed a search of the MEDLINE database (January 1997–April 2010) for all English‐language articles of in vitro and in vivo studies that evaluated everolimus, as well as abstracts from recent scientific meetings and the manufacturer. In transplantation, everolimus demonstrates immunosuppressive properties and has been used to prevent acute rejection in cardiac, liver, lung, and renal transplant recipients. It appears that this agent may be potent enough to allow for the minimization or removal of calcineurin inhibitors in the long‐term management of renal transplant recipients. In oncology, everolimus has been proven effective for the management of treatment‐resistant renal cell carcinoma. In cardiology, everolimus is available as a drug‐coated stent and is used in percutaneous coronary interventions for prevention of restenosis. In transplant recipients and patients with renal cell carcinoma, everolimus appears to have an extensive adverse‐event profile. The pharmacologic properties of everolimus differentiate this agent from other drugs used in these clinical areas, and its pharmacokinetic properties differentiate it from sirolimus.
Investigating housing tenures beyond homeownership: a study of informal settlements in Kolkata, India

Abstract Improving the lives of households in informal settlements is a major development challenge. Though ownership is the predominant housing tenure arrangement pursued in national and international housing policies, there is a growing consensus that forms of tenure beyond homeownership may provide greater benefits to some households. In Kolkata, informal settlements (bastis) are the primary source of affordable housing for the urban poor. Relying on detailed household survey data, we investigate the utility gained from the diversity of housing tenure arrangements in bastis and the factors that increase the length of time a household lives in a settlement, using multinomial logit and ordinary least squares regression specifications. Our analyses show that in comparison to ownership, leasing or renting benefits large households and new migrants. Our findings suggest that policies targeted at households who rent and lease, along with improving housing and supporting infrastructure, can be effective mechanisms for improving household well-being.
Moving Closer or Drifting Apart: Distributional Effects of Monetary Policy

The heating debate about increasing income inequality forces monetary policy makers and academia to (re‐)assess the nexus between (unconventional) monetary policy and inequality. We use a VAR framework to unveil the distributional effects of monetary policy and the role of redistribution in six advanced economies. While all of them experience an increase in Gini coefficients of gross income due to an expansionary monetary policy shock, only countries with relatively little redistribution display a significant response of net income inequality as well. To examine the underlying transmission channels we take a closer look at the sources of income, i.e. labor and capital income. Our findings suggest that the disproportional surge in capital income is the driving force behind the increase in net income inequality.
The effect of suicide-related Internet use on users' mental health.

BACKGROUND Previous studies have shown that suicide-related Internet use can have both negative and positive psychological effects. AIMS This study examined the effect of suicide-related Internet use on users' suicidal ideation, depression/anxiety tendency, and loneliness. METHOD A two-wave panel study of 850 Internet users was conducted via the Internet. RESULTS Suicide-related Internet use (e.g., browsing websites about suicide methods) had negative effects on suicidal ideation and depression/anxiety tendency. No forms of suicide-related Internet use, even those that would generally be considered positive, were found to decrease users' suicidal ideation. In addition, our results suggest that the greater the suicidal ideation and feelings of depression and loneliness of Internet users, the more they used the Internet. CONCLUSION Since suicide-related Internet use can adversely influence the mental health of young adults, it is necessary to take measures to reduce their exposure to such information.
Differential activity in left inferior frontal gyrus for pseudowords and real words: An event‐related fMRI study on auditory lexical decision

After Newman and Twieg ([ 2001 ]: Hum Brain Mapp 14:39–47) and others, we used a fast event‐related functional magnetic resonance imaging (fMRI) design and contrasted the lexical processing of pseudowords and real words. Participants carried out an auditory lexical decision task on a list of randomly intermixed real and pseudo Chinese two‐character (or two‐syllable) words. The pseudowords were constructed by recombining constituent characters of the real words to control for sublexical code properties. Processing of pseudowords and real words activated a highly comparable network of brain regions, including bilateral inferior frontal gyrus, superior, middle temporal gyrus, calcarine and lingual gyrus, and left supramarginal gyrus. Mirroring a behavioral lexical effect, left inferior frontal gyrus (IFG) was significantly more activated for pseudowords than for real words. This result disconfirms a popular view that this area plays a role in grapheme‐to‐phoneme conversion, as such a conversion process was unnecessary in our task with auditory stimulus presentation. An alternative view was supported that attributes increased activity in left IFG for pseudowords to general processes in decision making, specifically in making positive versus negative responses. Activation in left supramarginal gyrus was of a much larger volume for real words than for pseudowords, suggesting a role of this region in the representation of phonological or semantic information for two‐character Chinese words at the lexical level. Hum Brain Mapp 25:212–221, 2005. © 2005 Wiley‐Liss, Inc.
Road crash casualties: characteristics of police injury severity misclassification.

BACKGROUND In most countries, epidemiologic knowledge of road crash injury is mainly based on police data, as they very often are the only available data at the nation-wide level. However their validity is of some concern. We focus here on the police severity classification of 'serious' and 'slight' casualties in France. We want to know how the police classification compares with a trauma severity scale, so that we could correctly interpret police based studies. METHOD The study is based on the Rhône county (population 1.6 million) during the 1997 to 2001 period. Police data have been linked with a road trauma registry, so that both police and New Injury Severity Score (NISS) classifications are available on 14,342 casualties. The police classification of 'slight' and 'serious' casualties is compared with the registry classification grouped into NISS 1-15 and NISS 16-75 categories. We conduct multivariate analyses of the probability of police severity misclassification, over and under-classification, as a function of crash and casualty characteristics. RESULTS Kappa is estimated at 0.41; the sensitivity of the police classification is 72% and the positive predictive value is 35%. Pedestrian and motorcyclist casualties are the most likely to be over-classified (Relative Risk [RR] = 1.4 and RR = 1.2, respectively compared with car occupants). The 'rural police' are more likely to over-classify than the other police forces (RR = 3.1). Over-classification decreased during the 1997 to 2001 period whereas under- classification increased. CONCLUSION These misclassification characteristics must be kept in mind when interpreting severity results based on police data. We are working on obtaining unbiased nation-wide estimates of severity figures.
THERAPEUTIC RESULTS IN BRIEF‐INTERVAL UNILATERAL ECT

The study reported comprised 104 patients with endogenous depression who were given unilateral electroconvulsive therapy (ECT). The patients were divided into two equally large groups, one receiving two and the other four treatments per week. The latter group was selected openly, while the former had been collected by a double‐blind technique on a previous occasion. The severity of depression was assessed before the first treatment and on the days after the sixth and the last treatment, and at these times memory testing by WMS Forms I and II and EEG were also performed.
Model-based Evaluation: from Dependability Theory to Security

How to quantify security is a classic question in the security community that until today has had no plausible answer. Unfortunately, current security evaluation models are often either quantitative but too specific (i.e., applicability is limited), or comprehensive (i.e., system-level) but qualitative. The importance of quantifying security cannot be overstated, but doing so is difficult and complex, for many reason: the “physics” of the amount of security is ambiguous; the operational state is defined by two confronting parties; protecting and breaking systems is a cross-disciplinary mechanism; security is achieved by comparable security strength and breakable by the weakest link; and the human factor is unavoidable, among others. Thus, security engineers face great challenges in defending the principles of information security and privacy. This thesis addresses model-based system-level security quantification and argues that properly addressing the quantification problem of security first requires a paradigm shift in security modeling, addressing the problem at the abstraction level of what defines a computing system and failure model, before any system-level analysis can be established. Consequently, we present a candidate computing systems abstraction and failure model, then propose two failure-centric model-based quantification approaches, each including a bounding system model, performance measures, and evaluation techniques. The first approach addresses the problem considering the set of controls. To bound and build the logical network of a security system, we extend our original work on the Information Security Maturity Model (ISMM) with Reliability Block Diagrams (RBDs), state vectors, and structure functions from reliability engineering. We then present two different groups of evaluation methods. The first mainly addresses binary systems, by extending minimal path sets, minimal cut sets, and reliability analysis based on both random events and random variables. The second group addresses multi-state security systems with multiple performance measures, by extending Multi-state Systems (MSSs) representation and the Universal Generating Function (UGF) method. The second approach addresses the quantification problem when the two sets of a computing system, i.e., assets and controls, are considered. We adopt a graph-theoretic approach using Bayesian Networks (BNs) to build an asset-control graph as the candidate bounding system model, then demonstrate its application in a novel risk assessment method with various diagnosis and prediction inferences. This work, however, is multidisciplinary, involving foundations from many fields, including security engineering; maturity models; dependability theory, particularly reliability engineering; graph theory, particularly BNs; and probability and stochastic models.
Safety Outcomes of Apixaban Compared With Warfarin in Patients With End-Stage Renal Disease

Background: Current guidelines make no specific recommendations on the selection of direct oral anticoagulants for the prevention and treatment of venous thromboembolism in patients with end-stage renal disease (ESRD) receiving hemodialysis. Based on these guidelines, warfarin remains the anticoagulant of choice in these patients. Objective: To compare bleeding rates in patients receiving apixaban or warfarin with ESRD undergoing chronic hemodialysis. Methods: This was a single-center, retrospective, institutional review board–approved cohort analysis. Patients with ESRD undergoing chronic hemodialysis and receiving anticoagulation therapy with either apixaban or warfarin were included in this study. All data were collected from paper charts and electronic medical records and included documentation of bleeding events and related interventions. The primary outcome of this study was clinically relevant major bleeding events. Secondary outcomes included clinically relevant nonmajor bleeding events and minor bleeding events. Results: A total of 160 patients were included in this study (warfarin group, n = 120; apixaban group, n = 40). There were 7 major bleeding events in the warfarin group compared with zero in the apixaban group (P = 0.34). There were similar rates of clinically relevant nonmajor bleeding events (12.5% vs 5.8%, P = 0.17) and minor bleeding (2.5% vs 2.5%, P = 0.74) events in patients receiving apixaban and warfarin. Conclusions: There were no observed differences in bleeding rates in patients receiving apixaban compared with those receiving warfarin. Apixaban may be a cautious consideration in hemodialysis patients until there is further insight into the effect of subsequent, multiple doses on drug accumulation and clinical outcomes.
Acclimatization to high-variance habitats does not enhance physiological tolerance of two key Caribbean corals to future temperature and pH

Corals are acclimatized to populate dynamic habitats that neighbour coral reefs. Habitats such as seagrass beds exhibit broad diel changes in temperature and pH that routinely expose corals to conditions predicted for reefs over the next 50–100 years. However, whether such acclimatization effectively enhances physiological tolerance to, and hence provides refuge against, future climate scenarios remains unknown. Also, whether corals living in low-variance habitats can tolerate present-day high-variance conditions remains untested. We experimentally examined how pH and temperature predicted for the year 2100 affects the growth and physiology of two dominant Caribbean corals (Acropora palmata and Porites astreoides) native to habitats with intrinsically low (outer-reef terrace, LV) and/or high (neighbouring seagrass, HV) environmental variance. Under present-day temperature and pH, growth and metabolic rates (calcification, respiration and photosynthesis) were unchanged for HV versus LV populations. Superimposing future climate scenarios onto the HV and LV conditions did not result in any enhanced tolerance to colonies native to HV. Calcification rates were always lower for elevated temperature and/or reduced pH. Together, these results suggest that seagrass habitats may not serve as refugia against climate change if the magnitude of future temperature and pH changes is equivalent to neighbouring reef habitats.
Affirming the Self to Promote Agreement With Another

Two studies investigated the capacity of a self-affirmation intervention to lower a psychological barrier to conflict resolution. Study 1 used a role-play scenario in which a student negotiated with a professor for greater rewards for work on a collaborative project. A self-affirmation manipulation, in which participants focused on an important personal value, significantly reduced their tendency to derogate a concession offered by the professor relative to one that had not been offered. Study 2 replicated this effect and showed that the phenomenon did not depend on the self-affirmed participant’s experience of a heightened sense of deservingness or a tendency to make positive attributions about the professor. Distraction and explicit mood enhancement were also ruled out as mediators of the self-affirmation effect, which appears to stem from motivational rather than explicit cognitive processes.
Measuring the Constructs of an Islamic Human Development Model (I-Hdm) for Nigeria’s Rural Community

This paper seeks to validate the Islamic human development model (I-HDM) by providing empirical evidence on the interrelatedness of social justice and human rights (exogenous and the three contextalised factors of education, health, and income (endogenous) based on the Maqasid Shariah framework. Conventionally, human development is measured through education, income and health, however, other integral components of development such as social justice and human rights are not taken into consideration in this mainstream model. This paper employed quantitative research design and used a survey questionnaire to collect the data. The findings support the hypothesised model with the model fit, significant inter-factor relationship, and high overall internal consistency of 0.804. The findings further show that many people living in rural Nigeria are unaware of their social and economic rights and the government responsibility to fulfilling them.