Miracle-dz/UniFAR-FTUs huggingface dataset:
Biodegradation and in vivo biocompatibility of rosin: a natural film-forming polymer

The specific aim of the present study was to investigate the biodegradation and biocompatibility characteristics of rosin, a natural film-forming polymer. Both in vitro as well as in vivo methods were used for assessment of the same. The in vitro degradation of rosin films was followed in pH 7.4 phosphate buffered saline at 37°C and in vivo by subdermal implantation in rats for up to 90 days. Initial biocompatibility was followed on postoperative days 7, 14, 21, and 28 by histological observations of the surrounding tissues around the implanted films. Poly (DL-lactic-co-glycolic acid) (PLGA) (50∶50) was used as reference material for biocompatibility. Rate and extent of degradation were followed in terms of dry film weight loss, molecular weight (MW) decline, and surface morphological changes. Although the rate of in vitro degradation was slow, rosin-free films showed complete degradation between 60 and 90 days following subdermal implantation in rats. The films degraded following different rates, in vitro and in vivo, but the mechanism followed was primarily bulk degradation. Rosin films demonstrated inflammatory reactions similar to PLGA, indicative of good biocompatibility. Good biocompatibility comparable to PLGA is demonstrated by the absence of necrosis or abscess formation in the surrounding tissues. The study provides valuable insight, which may lead to new applications of rosin in the field of drug delivery.
Balance Performance in Male and Female Collegiate Basketball Athletes: Influence of Testing Surface

Sabin, MJ, Ebersole, KT, Martindale, AR, Price, JW, and Broglio, SP. Balance performance in male and female collegiate basketball athletes: Influence of testing surface. J Strength Cond Res 24(8): 2073-2078, 2010-The Star Excursion Balance Test (SEBT) is a simple and cost-effective balance test. Information on SEBT performance in athletic populations and under varying testing surfaces is needed to fully elucidate the clinical and training utility of this task. The purpose of this study was to examine SEBT performance in division I, collegiate basketball athletes (men = 9, women = 7) and in a healthy nonathlete control group (men = 7, women = 9). Each participant performed the SEBT with their dominant and nondominant limbs on stable and unstable testing surfaces while reaching in the anterior, medial, and posterior directions. No significant differences resulted between the dominant and nondominant limbs (p > 0.05). Significant differences were found between the basketball and control groups in all directions (p < 0.01) and the average reach score (p < 0.01) with the control group reaching 6-7% farther than the basketball group. A significant main effect for gender (collapsed across group and limb) was present in the posterior direction (p = 0.02). The SEBT performance in the medial and posterior directions and the average score were significantly (p < 0.01) reduced during testing on the unstable surface. These results suggest that athletic status may be a factor to consider when interpreting SEBT reach performance. Future research should examine the influence of SEBT testing in other athletic populations. Further, use of an unstable surface provided a greater challenge to balance. Additional research may provide insight into the role of using an unstable surface with the SEBT as part of an injury risk assessment and its use in identifying limb differences between injured and uninjured limbs.
Requirement for CD28 in the Effector Phase of Allergic Airway Inflammation1

Central to the pathogenesis of allergic airway inflammation are the activation and differentiation of T lymphocytes. This process requires the participation of the CD28 costimulatory receptor. Blockade of CD28 has been demonstrated to prevent inflammation and airway hyperreactivity in a murine model of asthma. Whether this is due specifically to defects in initial T cell activation or whether effector responses are also impaired has not been determined. Using adoptive transfer studies of Ag-specific lymphocytes, we demonstrate that CD28 has a critical role in both the induction and effector phase of allergic airway inflammation. Transfer of in vitro activated and Th2-differentiated Ag-specific lymphocytes from wild-type hosts restored inflammation, but not tissue eosinophilia in CD28-deficient recipients. Furthermore, similarly activated and differentiated CD28-deficient lymphocytes were ineffective at mediating inflammation in wild-type recipients. Secondary cytokine and proliferative responses of activated Th2 cells were highly dependent on CD28 in vitro. Moreover, eosinophil recruitment to both the lung and peritoneum is impaired by the lack of CD28, suggesting a generalized defect in the ability of eosinophils to accumulate at sites of inflammation in vivo. These data identify a novel role for CD28 in the effector phase of allergic airway inflammation and suggest that inhibition of this pathway may be a useful therapeutic intervention in previously sensitized individuals.
Increased levels of the T‐helper 22‐associated cytokine (interleukin‐22) and transcription factor (aryl hydrocarbon receptor) in patients with periodontitis are associated with osteoclast resorptive activity and severity of the disease

BACKGROUND AND OBJECTIVE Two new T-helper (Th) phenotypes have been recently described and named Th9 and Th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. This study was aimed to assess whether Th9 and Th22 lymphocytes, through interleukin (IL)-9 and IL-22 production, respectively, are associated with the severity of periodontitis and bone resorption. MATERIAL AND METHODS Gingival crevicular fluid samples and biopsies were obtained from patients with moderate-to-advanced chronic periodontitis and gingivitis, and healthy controls. The levels for the Th9 and Th22-associated cytokines and master-switch transcription factors Spi-B and aryl hydrocarbon receptor (AhR) were quantified by enzyme-linked immunosorbent assay, real-time reverse-transcription quantitative polymerase chain reaction and flow cytometry. In addition, the osteoclast activity in response to tissue homogenates from periodontitis and healthy samples was analyzed quantifying the number of TRAP-positive cells and areas of bone resorption pits produced, in the presence or absence of recombinant human IL-22 and anti-IL-22 neutralization antibody. RESULTS Higher levels of IL-22 and AhR were detected in patients with periodontitis compared with gingivitis and healthy individuals. In addition, higher levels of IL-9 and Spi-B were detected in gingivitis patients compared with periodontitis and healthy individuals. In patients with periodontitis, a significant positive correlation was detected between secreted levels of IL-22 and clinical attachment level of the sampled periodontal pockets. When osteoclasts were exposed to tissue homogenates obtained from patients with periodontitis, higher levels of resorptive activity were observed as compared with the same cells exposed to tissue homogenates obtained from healthy individuals, and this increment was dependent on the presence and neutralization of IL-22. CONCLUSION Increased levels of IL-22 produced by Th22 lymphocytes are associated with the pathogenesis of periodontitis, in particular, with osteoclast resorptive activity and severity of disease.
ABSTRACT On the Robustness of Brain Gain Estimates

Recent theoretical studies suggest that migration prospects can raise the expected return to human capital and thus foster education investment at home or, in other words, induce a brain gain. In a recent paper (Beine, Docquier and Rapoport, Economic Journal, 2008) we used the Docquier and Marfouk (2006) data set on emigration rates by education level to examine the impact of brain drain migration on gross (pre-migration) human capital formation in developing countries. We found a positive effect of skilled migration prospects on human capital growth in a cross-section of 127 developing countries, with an elasticity of about 5 percent. In this paper we assess the robustness of our results to the use of alternative brain drain measures, definitions of human capital, and functional forms. We find that the results hold using the Beine et al. (2007) alternative brain drain measures controlling for whether migrants acquired their skills in the home or in the host country. We also regress other indicators of human capital investment on skilled migration rates and find a positive effect on youth literacy while the effect on school enrolment depends on the exact specification chosen.
Selective ligand activity at Nur/retinoid X receptor complexes revealed by dimer‐specific bioluminescence resonance energy transfer‐based sensors

Retinoid X receptors (RXRs) play a role as master regulators because of their capacity to form heterodimers with other nuclear receptors (NRs). Accordingly, retinoid signaling is involved in multiple biologic processes, including development, cell differentiation, metabolism, and cell death. However, the role and function of RXRs in different heterodimer complexes remain unidentified, mainly because most RXR drugs (called rexinoids) are not selective of specific heterodimer complexes. The lack of selectivity strongly limits the use of rexinoids for specific therapeutic approaches. To better characterize rexinoids at specific NR complexes, we have developed and optimized luciferase (Luc) protein complementation (PCA)‐based bioluminescence resonance energy transfer (BRET) assays that can directly measure recruitment of a coactivator (CoA) motif fused to yellow fluorescent protein (YFP) by specific NR dimers. To validate the assays, we compared rexinoid modulation of CoA recruitment by the RXR homodimer and by the heterodimers Nur77/RXR and Nurr1/RXR. Results revealed that some rexinoids display selective CoA recruitment activities with homo‐ or heterodimer complexes. In particular, SR11237 (BMS649) has stronger potency for recruitment of CoA motif and transcriptional activity with the heterodimer Nur77/RXR than other complexes. This technology should be useful in identifying new compounds with specificity for individual dimeric species formed by NRs. —Giner, X. C. , Cotnoir‐White, D. , Mader, S. , Lévesque, D. Selective ligand activity at Nur/retinoid X receptor complexes revealed by dimer‐specific bioluminescence resonance energy transfer‐based sensors. FASEB J. 29, 4256‐4267 (2015). www.fasebj. org
In Vitro Interaction of the HIV Protease Inhibitor Ritonavir with Herbal Constituents: Changes in P-gp and CYP3A4 Activity

The purpose of this study was to evaluate in vitro interactions of commercially obtained pure herbal constituents with p-glycoprotein P-gp and cytochrome P-450 3A4 (CYP3A4) activities, which can further modulate the transcellular transport and metabolism kinetics of orally administered drugs. Caco-2 cells grown in the presence of 0.25 μmol/L 1α,25-dihydroxy vitamin D3 and multidrug-resistant 1 (MDR1) transfected MDCK cells were used as models to evaluate the effect of purified herbal constituents (quercetin, hypericin, hyperforin from St. John’s wort, kaempferol from ginseng, silibinin from milk thistle, and allicin from garlic) on P-gp–mediated efflux of the human immunodeficiency virus (HIV) protease inhibitor ritonavir. In addition, the inhibitory effect of these constituents on CYP3A4-mediated metabolism was determined by using cortisol as a model compound. Silibinin and hyperforin did not significantly alter cellular uptake of 3H-ritonavir in Caco-2 cells. A similar result was also observed for silibinin when tested in MDR1-MDCK cells. Quercetin, hypericin, and kaempferol exhibited a remarkable inhibition of P-gp–mediated efflux of ritonavir by increasing its cellular uptake in these models. These values were also comparable with the inhibitory effect of quinidine in Caco-2 cells, a well-known inhibitor of P-gp, on ritonavir efflux from Caco-2 cells. Allicin exhibited a concentration-dependent inhibition of ritonavir efflux when tested on MDR1-MDCK cells. There was a significant decrease in the Apical to Basal/Basal to Apical (AP-BL/BL-AP) transport ratio of ritonavir in presence of hypericin, kaempferol, and quercetin. These herbal constituents inhibited the CYP3A4 activity when tested with the Vivid CYP3A4 assay kit, whereas silibinin did not alter cortisol metabolism. Hypericin showed a significant inhibition in reduced nicotinamide adenine dinucleotide phosphate (NADPH)–dependent metabolism of cortisol with 64.6% of intact drug at the end of a 1-hour study. Similarly, kaempferol and quercetin also caused substantial inhibition of cortisol metabolism with 89.7% and 90.1% of intact cortisol, respectively, compared with 45.9% in the control. Prolonged exposure of quercetin resulted in significant increase of mRNA expression of both MDR1 and CYP3A4 levels in Caco-2 cells. However, hyperforin caused upregulation of CYP3A4 and downregulation of MDR1, whereas the effect of silibinin and kaempferol remained inconclusive on these gene expressions. Hypericin, kaempferol, quercetin, and allicin inhibit the efflux and CYP3A4-mediated metabolism of xenobiotics in vitro. Hence, this study warns against the use of herbal constituents along with prescribed HIV protease inhibitors that are substrates for P-gp and/or CYP3A4.
UNIONS, TRAINING, AND WAGES: EVIDENCE FOR BRITISH MEN *

In this paper we investigate the impact of trade unions on work-related training and wage formation for a sample of full-time men in Britain. We use data from the British Household Panel Survey for the period 1991-1996. Our main findings are that, relative to non-union workers, union-covered workers (i) are more likely to receive training, (ii) receive more days of training than their non-unionised counterparts, and (iii) experience a higher wage growth and a greater return to training. While findings (i) and (ii) have been found with other British data sets, the other results are new. They suggest a rethinking is warranted of the conventional view that union wage formation in Britain reduces the incentives to acquire work-related training.
Comparison of a food frequency questionnaire with an online dietary assessment tool for assessing preschool children's dietary intake.

BACKGROUND It is important to assess the dietary habits of children as they progress from early childhood to adolescence. However, there are few validated tools available to assess the diet of this group of children. The present study aimed to investigate the relative validity of a food frequency questionnaire (FFQ) developed for use in a biennial longitudinal study. METHODS The parents of 216 Belgian-Flemish preschool children completed a FFQ (including questions on 77 food groups) and an online dietary assessment tool for three nonconsecutive days on their child's diet. Intakes of energy, macronutrients, fibre, calcium and 20 food groups recorded by both methods were compared using Spearman's correlations, Wilcoxon's signed rank test and Bland and Altman analyses. RESULTS At the group level, good agreement was found for energy, fat and protein intake, an overestimation was found for carbohydrates (5.6%) and fibre (13.3%), and an underestimation was found for calcium (9%). For the food groups, overestimation as well as underestimation and good agreement were found, although overestimation was most common. Good agreement was found in ranking the children's fruit (r = 0.53), beverages (r = 0.58-0.76), soup (r = 0.51), breakfast cereals (r = 0.55) and calcium (r = 0.59) intake according to both methods. CONCLUSION The results obtained revealed that the FFQ was a useful alternative for estimating energy and macronutrient intake at a group level; however, when used to estimate fibre and calcium intake, respectively, over- and underestimation need to be considered. The short reference period (3 days) may have compromised agreement in ranking and agreement at the individual level.
Interleukin 2 enhances specific and nonspecific immune responses in experimental Chagas' disease

Mice infected with Trypanosoma cruzi develop an early and profound immunosuppression of responses to heterologous antigens. Recently it has been demonstrated that this immunosuppression is linked, in part, to deficiency in the production of interleukin 2 (IL-2), and that the addition of IL-2 to cultures of normally unresponsive spleen cells from infected mice will restore responsiveness to sheep erythrocytes (SRBC) and enhance parasite-specific immune responses. In the present study, the effect of administration of ultrapure or recombinant IL-2 on immune responses to SRBC and parasite-specific responses in vivo was examined. It was found that a single injection of 1,500 U of IL-2 provided at the same time as SRBC more than doubled the number of direct plaque-forming cells to SRBC and that multiple injections of 1,500 U of IL-2 were no more restorative than a single injection. Anti-SRBC responses of normal mice were unaffected by injection of IL-2. Single or multiple injections of recombinant human IL-2, with and without gelatin, into highly susceptible C3H(He) mice induced greater parasite-specific immunity as reflected by significantly reduced levels of parasitemia and increased longevity. Three injections of 1,500 U each of recombinant human IL-2 on days 10, 14, and 18 was found to be the most efficacious in reducing parasitemia and increasing longevity. Injection of IL-2 with gelatin did not enhance the effect of IL-2 alone.
No transmission of blood-borne viruses among hospital staff despite frequent blood exposure.

INTRODUCTION Exposure to blood and body fluids (BBF) is a major concern for health-care workers (HCWs) and implies a risk of infection with blood-borne pathogens. However, in Denmark, no exposure incidence studies among HCWs have been reported for the past ten years. The aims of this study were to provide an updated evaluation of the annual frequency of registered exposures during the 2003-2012 period, the prevalence and incidence of transmission of HIV, HBV and HCV among HCWs, the prevalence of HIV, HBV and HCV among source patients, the follow-up by HBV vaccination and blood sampling in exposed HCWs and, finally, reporting habits. MATERIAL AND METHODS All registered first-time cases of BBF exposure at Odense University Hospital during the 2003-2012 period were included. The exposed HCW and source patient were linked to a laboratory database to obtain the test results for HIV, HBV, HCV and the anti-HBs level at baseline and after exposure. For 2012, a detailed analysis of BBF exposure was performed. RESULTS A total of 2,274 first-time BBF exposures were analysed. We observed a 35% increase in the reported incidence of exposures in the period. The prevalence and incidence of HIV, HBV and HCV among HCWs was zero. The prevalence of anti-HIV among source patients was 0.9%, HBsAg 1.2% and anti-HCV/HCV-RNA 3.8%. In 2003-2012, 31.3% of the tested HCWs had an anti-HBs ≥ 10 IU/l at baseline and this increased to 76.1% after vaccination. In 2012, 95% of the HCWs had blood samples at the time of exposure, 35% had a three-month blood test and 17% had a six-month test. CONCLUSION Despite a high rate of exposure to BBF among HCWs, the risk of infection was low. FUNDING no external funding received. TRIAL REGISTRATION not relevant.
Should Private Sector Participate in Public Private Partnerships? Evidence from China and India

In this paper, we investigate whether Public Private Partnerships (PPPs), with readily pledgable Government assets, reduce the underinvestment problems faced by private sector firms in emerging markets. We analyse investment-cash flow sensitivity and its determinants for the partnering private sector firms in China and India. We find that, PPP announcements create wealth to the partnering private sector firms in both countries. However, the nature of firm that undertakes PPP projects varies. In China, older, matured, better valued firms with high cash inflows engage in PPPs while in India, younger, higher debt burden, cash-constrained firms engage in PPPs. This indicates that underinvestment problem is more significant reason for PPP investments in India compared to China. In the long run, PPP involvement reduces investment-cash flow sensitivity in both countries’ private sector firms, however, higher reliance on Government and political connections are more beneficial in China compared to India.
Hemostatic resuscitation during surgery improves survival in patients with traumatic-induced coagulopathy.

BACKGROUND Although hemostatic resuscitation with a 1:1 ratio of fresh-frozen plasma (FFP) to packed red blood cells (PRBC) after severe hemorrhage has been shown to improve survival, its benefit in patients with traumatic-induced coagulopathy (TIC) after >10 units of PRBC during operation has not been elucidated. We hypothesized that a survival benefit would occur when early hemostatic resuscitation was used intraoperatively after injury in patients with TIC. METHODS A 7-year retrospective study of patients with emergency department diagnosis of TIC after transfusion of >10 units of PRBC in the operating room. TIC was defined as initial emergency department international normalized ratio > 1.2, prothrombin time > 16 seconds, and partial thromboplastin time > 50 seconds. Patients were divided into FFP:PRBC ratios of 1:1, 1:2, 1:3, and 1:4. Patients with diagnosis of TIC who received transfusion of both FFP and PRBC during surgery were included. Other variables evaluated included age, gender, mechanism of injury, initial base deficit, mean operative time, trauma intensive care unit length of stay (TICU LOS) and Injury Severity Score. The primary outcome measure evaluated was the impact of the early FFP:PRBC ratio on mortality. RESULTS Four hundred thirty-five patients underwent emergency operations postinjury and received FFP with >10 units of PRBC in the operating room; 135 (31.0%) of these patients had TIC and 53 died (39.5% mortality). Mean operative time was 137 minutes (SD +/- 49). There were no differences with regard to age, gender, mechanism of injury, initial base deficit, or Injury Severity Score among all groups. A significant difference in mortality was found in patients who received >10 units of PRBC when FFP:PRBC ratio was 1:1 versus 1:4 (28.2% vs. 51.1%, p = 0.03). Intermediate mortality rates were noted in patients with 1:2 and 1:3 ratios (38% and 40%, respectively). From a linear regression model, 13 days of increased TICU LOS was observed among 1:4 group compared with 1:1 group (p < 0.01). CONCLUSION TIC is common after severe injury and is associated with a high mortality in patients transfused with >10 units of PRBC during surgery. Early hemostatic resuscitation during first hours after injury improves survival with shorter TICU LOS in patients with TIC.