Miracle-dz/UniFAR-FTUs huggingface dataset:
Two Pathways Underlying the Effects of Physical Education on Out-of-School Physical Activity

ABSTRACT One primary goal of physical education (PE) is to promote students’ lifelong physical activity (PA). This goal implies that PE should not only improve students’ PA in PE classes but also promote their PA outside of the school known as the “PE effect. ” Purpose: In this study, we proposed a two-pathway model of the “PE effect” and hypothesized that learning in PE and positive motivational experience in PE are two possible pathways through which the “PE effect” emerges. We tested the tenability of this two-pathway model from the perspective of knowledge learning and autonomous motivation in PE. Methods: A total of 394 eighth-grade students from five schools provided data on PA knowledge, out-of-school PA, and autonomous motivation toward PE and PA. Structural equation modeling was used to test the two-pathway model. Results: Results showed that the students’ knowledge had a direct effect (β = .18, p < .05) on their autonomous motivation for PA and an indirect effect (β = .02, p < .05) on out-of-school PA through influencing autonomous motivation toward PA. Students’ autonomous motivation for PE had a direct effect (β = .41, p < .05) on their autonomous motivation toward PA and an indirect effect (β = .04, p < .05) on out-of-school PA through autonomous motivation toward PA. Conclusion: These results indicate that the two-pathway model is tenable in terms of knowledge learning and autonomous motivation in PE and imply that teaching knowledge in an autonomy-supportive PE environment may be an effective way to promote students’ out-of-school PA.
Experimental study of the synergistic effect and network regulation mechanisms of an applied combination of BMP‐2, VEGF, and TGF‐β1 on osteogenic differentiation

The study aimed to explore the osteogenic effect induced by the combined use of bone morphogenetic protein‐2 (BMP‐2), vascular endothelial growth factor (VEGF), and transforming growth factor‐β1 (TGF‐β1), attain the best combination for osteogenic quality and efficiency, and explore the network regulation mechanisms of induced osteogenesis. MC3T3‐E1 cells were cultured in vitro, and BMP‐2, VEGF, and TGF β1 were added to osteogenic induction mediums in different combinations to conduct experiments. At 7 and 14 days, the alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of the applied BMP‐2 and VEGF combination were deeper and the quantitative analysis were higher than those of the other groups. After optimizing the time–effect relationship of the combined application, with BMP‐2, VEGF, and TGF‐β1 adding in the early stage and BMP‐2 and VEGF adding in the late, the ALP and ARS staining of these groups were deeper and the quantitative analyses were meaningfully higher than the BMP‐2 and VEGF combination group at 7 and 14 days. The expression of the RUNX2 gene and the Smad1 signaling pathway in the optimized combination group was also significantly higher. The results demonstrate that the combination of BMP‐2, VEGF, and TGF‐β1 applied according to the time–effect relationship can significantly promote osteogenic differentiation mainly through the classical BMP‐receptor‐Smad signal pathway.
Speech disfluencies in bilingual Yiddish-Dutch speaking children

ABSTRACT In this study, we investigated the frequency and types of stuttering-like (SLD) and other (OD) disfluencies in 59 typically developing bilingual Yiddish-Dutch (YD) speaking children. Participants were divided in two age categories: 6.01–7.07 and 9.00–10.04-year-olds. All children (1) were successive, bilingual YD speaking, (2) had Yiddish as their dominant language and (3) were sufficiently intelligible in both languages. A conversation sample of at least 300 syllables was collected in each of the two languages. The main findings in this study were (a) the total amount of SLD as well as OD were significantly higher in the non-dominant language. For the SLD, this was mainly caused by the higher frequency of monosyllabic word and syllable repetitions. For the OD, almost all disfluency types seem to have contributed to this. (b) The total amount of OD was significantly higher in the older group of bilingual YD children than in the younger group. This was primarily due to higher frequencies of phrase repetitions, lexical revisions and unfinished words. (c) The monolingual diagnostic guideline of three SLD per 100 words as a means to label stuttering cannot be used in this bilingual population. The majority of the non-stuttering children scored higher than the standard 3% SLD in both languages. In the dominant Yiddish language, 27 children (46%) scored above this percentage, in the non-dominant Dutch language, 46 children (78%). We conclude that bilingual YD-speaking children have a higher frequency of speech disfluencies in comparison to monolingual children. Consequently, monolingual stuttering guidelines cannot be used in this bilingual population.
Hand hygiene: knowledge and skill of caregivers in the hematopoietic stem cell transplantation.

OBJECTIVE to identify the knowledge and ability of caregivers on hand hygiene in hematopoietic stem cell transplantation. METHOD a prospective cross-sectional study. A questionnaire was used to identify the knowledge and it was observed the hygiene technique performance. Data were collected in two units (autologous and allogeneic transplant). RESULTS the 37 participants recognized the importance of hand hygiene and 95.5% related to removal of dirt or infection prevention. 91.9% stated that it was important to clean their hands with soap and water when entering and leaving the room, and 64.9% understood that it was necessary to apply alcoholic solution after washing. On average, the caregivers scored 6.16 steps, when demonstrating the washing technique and 3.91 steps in the friction with alcoholic solution. CONCLUSION although they recognize the importance of the procedure, there are deficits related to the moment and the correct way of doing it, evidencing the need for strategies aimed at improving this process.
Male Partner Linkage to Clinic-based Services for Sexually Transmitted infections and HIV.

BACKGROUND Home-based HIV testing and education has increased HIV test uptake and access to health services among men. We studied how a home-based antenatal intervention influenced male partner utilization of clinic-based HIV and sexually transmitted infection (STI) services, linkage to HIV care and medical circumcision. METHODS We conducted a secondary analysis within a randomized controlled trial of pregnant women attending antenatal care in Kenya. Women and their male partners received either a home-based couple intervention or an invitation letter for clinic-based couple HIV testing. The home-based intervention included education on STI symptoms, STI and HIV treatment and male circumcision for HIV prevention. Male self-reported outcomes were compared using relative risks at 6 months postpartum RESULTS: Among 525 women, we reached 487 of their male partners (93%); 247 men in the intervention arm and 240 men in the control arm. Men who received the intervention were more likely to report an STI consultation [n=47 vs. 16; RR=1.59; 95%CI=1.33-1.89]. Among 23 men with newly diagnosed HIV, linkage to HIV care was reported by 4 of 15 in the intervention (3 men had missing linkage data) and 3 of 5 men in the control arms [RR=0.66; 95%CI: 0.34-1.29 ]. While the intervention identified 3 times more men with new HIV infection, the study lacked power to find significant differences in linkage to HIV care. Few eligible men sought medical circumcision (4 of 72 intervention and 2 of 88 control). CONCLUSIONS Home-based couple education and testing increased STI consultations among male partners of pregnant women, but appeared insufficient to overcome the barriers involved in linkage to HIV care and medical circumcision.
Exosome-Mediated MiR-155 Transfer Contributes to Hepatocellular Carcinoma Cell Proliferation by Targeting PTEN

Background Most eukaryocytes release nano vesicles (30–120 nm), named exosomes, to various biological fluids such as blood, lymph, and milk. Hepatocellular carcinoma (HCC) is one of the tumors with the highest incidence rate in primary malignant carcinoma of the liver. However, the mechanism of HCC proliferation remains elusive. In this study, we aim to explore whether HCC cell-derived exosomes affect the proliferation of cancer cells. Material/Methods Exosomes were isolated from HCC cells by ultracentrifugation and were visualized the phenotype by transmission electron microscopy. Cell proliferation was detected by Cell Counting Kit-8 assays and EdU (5-ethynyl-2-deoxyuridine) incorporation assays. Dual-luciferase assays were performed to validate the paired correlation of miR-155 and 3′-UTR of PTEN (gene of phosphate and tension homology deleted on chromosome 10). A xenograft mice model was constructed to verify the effect of exosome-mediated miR-155 on cell proliferation in vivo. Results Our finding showed that miR-155 was enriched in exosomes released from HCC cells. The exosome-containing miR-155 transferred into new HCC targeted cells and lead to the elevation of HCC cells’ proliferation. Besides, the exosomal miR-155 directly bound to 3′-UTR of PTEN leading to the reduction of relevant targets in recipient liver cells. The knockdown of PTEN attenuated the proliferation of HCC cells treated with the exosomal miR-155. Moreover, nude-mouse experiment results revealed a promotional effect of the exosomal miR-155 on HCC cell-acquired xenografts. Conclusions Our study indicated that exosomal-specific miR-155 transfers to adjacent and/or more distant cells and stimulates the proliferation of HCC cells.
The Shared Subjective Frames of Interdisciplinary Practitioners Involved in Function-Focused Care in a Nursing Home: Q-Methodology.

BACKGROUND An interdisciplinary team-based approach in nursing homes has been suggested in the literature as a strategy for delaying functional decline in residents. Function-focused care is a philosophy-based approach in which interdisciplinary practitioners assess functional capacity and help older adults to optimize and maintain their remaining abilities. PURPOSE This study explored and described the shared subjective frames of interdisciplinary practitioners as regards function-focused care for nursing home residents. METHODS Q-methodology was used to analyze the subjectivity of each factor of function-focused care for nursing home residents. Data were collected from August to September 2016. Thirty-four Q-statements were selected and scored by the 30 interdisciplinary practitioners on a 9-point scale with a normal distribution. Data were analyzed using the PQ Method 2.33 program. RESULTS The results revealed four factors of function-focused care, including (a) using a wait-and-see approach to encourage self-care, (b) maintaining interactive communications to identify and respond to changes, (c) reinforcing residents' inner and outer strengths for homeostasis, and (d) using a tailored approach based on comparisons between the past and the present. Shared subjectivity may provide an important collaborative framework to identify and solve complex problems related to the functional needs of nursing home residents. CONCLUSIONS The results of this study elucidate the subjectivities of interdisciplinary practitioners and better enable their provision of effective care in support of the remaining functional abilities of older adults living in nursing homes. The findings may be used as a reference to establish communication methods and shared documentation for interdisciplinary practitioners in nursing homes and construct interdisciplinary function-focused care practice guidelines.
Access to RNA-sequencing data from 1,173 plant species: The 1000 Plant transcriptomes initiative (1KP)

Abstract Background The 1000 Plant transcriptomes initiative (1KP) explored genetic diversity by sequencing RNA from 1,342 samples representing 1,173 species of green plants (Viridiplantae). Findings This data release accompanies the initiative's final/capstone publication on a set of 3 analyses inferring species trees, whole genome duplications, and gene family expansions. These and previous analyses are based on de novo transcriptome assemblies and related gene predictions. Here, we assess their data and assembly qualities and explain how we detected potential contaminations. Conclusions These data will be useful to plant and/or evolutionary scientists with interests in particular gene families, either across the green plant tree of life or in more focused lineages.
Assessment of survival and prognostic factors in metastatic colorectal cancer patients treated with first-line bevacizumab-based therapy.

PURPOSE Colorectal cancer (CRC) is a significant cause of cancer mortality worldwide. Survival has improved with bevacizumab in metastatic CRC treatment. Our purpose was to analyse survival and prognostic factors in metastatic CRC patients treated with first-line bevacizumab-based treatment. METHODS Files of CRC patients were examined retrospectively and 360 patients treated with first-line bevacizumab were included. Objective response rates (ORRs), median progression-free and overall survival (PFS and OS) of the patients were calculated. Survival was analyzed with the Kaplan-Meier method. Log-rank test and Cox regression model were used for univariate and multivariate analyses, respectively. RESULTS Median age at diagnosis was 59.5 years. Of the patients 74.4% had initially stage IV disease. Median PFS was 8.5 months, median OS 25.3 months and ORR was 51.4%. ORRs, median PFS and OS of KRAS mutant and wild-type or unknown patients were statistically similar. In left-sided disease, median PFS and OS (9.6 and 27.1 months) were superior compared to right-sided disease (7.3 and 19.4 months) (p=0.005 and 0.02, respectively). Primary disease location, histopathologic grade, primary surgery and metastasectomy affected OS significantly. Histopathologic grade (hazard ratio=1.77, p=0.002) and metastasectomy (hazard ratio=0.48, p=0.001) were independent prognostic factors. CONCLUSIONS Our study confirmed that after bevacizumab-based treatment, KRAS status might not be a prognostic factor. We have also shown that left CRC have more favorable outcomes than right CRC in bevacizumab therapy. Additionally, even in metastatic setting histopathologic grade of the primary CRC together with metastasectomy are independent prognostic factors.
Vocal symptoms in pediatric population: Validation of the Brazilian version of the Pediatric Vocal Symptoms Questionnaire.

PURPOSE To validate the Pediatric Vocal Symptoms Questionnaire (PVSQ) for Brazilian Portuguese. METHODS The study was approved by the Ethics Committee (758,309). A total of 716 individuals participated in this research, of which 367 were children and adolescents, with and without vocal alteration, aged 6-18 years, and 349 were parents/guardians, who responded to the final version of the instrument. Among the interviewed of this version, 272 participated in the test-retest, and 32, of the sensitivity. Children and adolescents aged 6-18 years responded to the self-evaluation version of the PVSQ, and their parents/guardians to its parental version. RESULTS The PVSQ showed acceptable reliability and reproducibility for the Brazilian population and sensitivity to vocal treatment. CONCLUSION The PVSQ was validated for Brazilian Portuguese, being a good instrument of vocal self-evaluation, both in the parental version and in the self-evaluation version.
Determination of haemoglobin A1c levels using high-performance liquid chromatography of bloodstains

This study aimed to determine haemoglobin A1c (HbA1c) levels in bloodstains shed on glass and fabric surfaces on specified test dates. Blood samples were taken from 26 patients (13 diabetic and 13 non-diabetic). Initial HbA1c levels were detected by using high-performance liquid chromatography (HPLC), and bloodstains were created on both cotton fabric and glass surfaces. Samples were processed at different ages (0, 7, 14, 28 and 56 days) by diluting distilled water and then measuring HbA1c levels by HPLC again. In all stains, HbA1c levels could be determined by using HPLC, but there was a moderate rise in accordance with the age of the stains. A statistically significant difference was found for bloodstains on clothes compared to those on glass surfaces. Receiver operating curve analysis found a sensitivity of 1.0 and specificity of 0.923 (cut-off 6.55) for glass surfaces on the seventh day; a sensitivity of 1.0, a specificity of 0.846 (cut-off 6.45) for clothes on the seventh day; a sensitivity of 1.0 and a specificity of 0.923 (cut-off 6.85) for clothes on the 56th day; and a sensitivity of 1.0 and a specificity of 0.846 (cut-off 7.55) for glass surfaces on the 56th day. In conclusion, this study found that HbA1c levels could be measured with high reliability from forensic bloodstains by using HPLC. Thus, in cases where DNA data banks cannot identify individuals, it would make sense to turn to those who have a medical history of diabetes among the suspects with the results of high HbA1c levels.
A systematic evaluation and optimization of automatic detection of ulcers in wireless capsule endoscopy on a large dataset using deep convolutional neural networks

Compared with conventional gastroscopy which is invasive and painful, wireless capsule endoscopy (WCE) can provide noninvasive examination of gastrointestinal (GI) tract. The WCE video can effectively support physicians to reach a diagnostic decision while a huge number of images need to be analyzed (more than 50 000 frames per patient). In this paper, we propose a computer-aided diagnosis method called second glance (secG) detection framework for automatic detection of ulcers based on deep convolutional neural networks that provides both classification confidence and bounding box of lesion area. We evaluated its performance on a large dataset that consists of 1504 patient cases (the largest WCE ulcer dataset to our best knowledge, 1076 cases with ulcers, 428 normal cases). We use 15 781 ulcer frames from 753 ulcer cases and 17 138 normal frames from 300 normal cases for training. Validation dataset consists of 2040 ulcer frames from 108 cases and 2319 frames from 43 normal cases. For test, we use 4917 ulcer frames from 215 ulcer cases and 5007 frames from 85 normal cases. Test results demonstrate the 0.9469 ROC-AUC of the proposed secG detection framework outperforms state-of-the-art detection frameworks including Faster-RCNN (0.9014) and SSD-300 (0.8355), which implies the effectiveness of our method. From the ulcer size analysis, we find the detection of ulcers is highly related to the size. For ulcers with size larger than 1% of the full image size, the sensitivity exceeds 92.00%. For ulcers that are smaller than 1% of the full image size, the sensitivity is around 85.00%. The overall sensitivity, specificity and accuracy are 89.71%, 90.48% and 90.10%, at a threshold value of 0.6706, which implies the potential of the proposed method to suppress oversights and to reduce the burden of physicians.
Baseline mucus cytokines predict 22‐item Sino‐Nasal Outcome Test results after endoscopic sinus surgery

Mucus cytokines have been linked to baseline metrics of quality of life and olfactory function in patients with chronic rhinosinusitis (CRS). However, their potential utility in predicting postoperative outcomes has not been assessed. Therefore, in this study we evaluated the role of mucus cytokines in predicting 22‐item Sino‐Nasal Outcomes Test (SNOT‐22) scores after endoscopic sinus surgery (ESS) in a prospective cohort of CRS patients.
Chronic arsenic toxicity and it's relation with nutritional status: A Case Study in Purabasthali-II, Burdwan, West Bengal, India

Arsenic is a major environmental pollutant and exposure through environmental, occupational and medicinal sources. Exposure through drinking water is major public health problem affecting most of the areas of our states, one such arsenic affected area is Purabasthali blockII, Burdwan, West Bengal, India. Reviewed of literature indicates that the chronic arsenic exposure increased risk for various forms of cancer and numerous noncancerous effects including diabetes, skin diseases, chronic cough, and toxic effects on liver, kidney, cardiovascular system, and peripheral and central nervous systems. Present survey also shows similar diseases pattern mostly skin disease and hair falling along with other symptoms. The situation is particularly severe in places where low socioeconomics status, poor literacy and malnutrition are predominant. Early stage of detection and enhancement of awareness and subsequent improvement of nutritional status can help to relief to the affected population. The present article deals with the arsenic predominance and also establishes the relation between water depth and intensity at arsenic toxicities, subsequently their detoxification through normal balance diet.
Can’t forget: disruption of the right prefrontal cortex impairs voluntary forgetting in a recognition test

ABSTRACT The causal influence of the dorsolateral prefrontal cortex (DLPFC) in voluntary forgetting remains unclear. Here, we employed repetitive transcranial magnetic stimulation (rTMS) over the right DLPFC to temporarily disrupt function of this brain region and examined its influence on an item-method directed forgetting (DF) task with both neutral and negative emotional memories. Participants were assigned to either an active or a sham rTMS group, in which we administered stimulation for 20 min before the DF task. We then examined the explicit and implicit DF effects with an explicit recognition and an implicit word completion test. We found that while participants in the sham group showed the classic DF effects in both explicit and implicit memory tests, temporally disrupting activity of the right DLPFC selectively reduced the DF effect on explicit recognition, but not on implicit word completion test. Our findings provide novel evidence that the right DLPFC plays a causal role in voluntary forgetting and support the direct inhibition account of voluntary memory control. Intriguingly, preserved implicit DF effects in the active stimulation group suggest that unintentional expressions of unwanted memories may be more sensitive to DF and less dependent on the right DLPFC.