Miracle-dz/UniFAR-FTUs huggingface dataset:
Steady-state concentrations of messenger ribonucleic acid encoding cytochrome P450 side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase/delta 5,delta 4 isomerase in ovine corpora lutea during the estrous cycle.

The objective of these experiments was to determine the pattern of mRNA expression for cytochrome P450 side-chain cleavage (P450scc) and 3 beta-hydroxysteroid dehydrogenase/delta 5,delta 4 isomerase (3 beta-HSD) during luteinization of the follicle and in ovine luteal tissue on Days 3, 6, 9, 12, and 15 of the estrous cycle. Mean concentration of mRNA for P450scc was not different in follicles collected 4 or 24 h after the onset of estrus but increased (p < 0.05) 3-fold by 48 h (corpus hemorrhagicum). With the methods used, mRNA for 3 beta-HSD was not detected until after ovulation and formation of the corpus hemorrhagicum (48 h after onset of estrus). In luteal tissue, mean concentration of mRNA for P450scc increased from Days 3 to 9 (p < 0.05) and had decreased (p < 0.05) by Day 15. Mean concentration of mRNA for P450scc was higher (p < 0.05) in small luteal cells on Day 9 than on Day 15, with values on Days 6 and 12 being intermediate. In large luteal cells, mean concentrations of P450scc mRNA increased (p < 0.05) between Days 6 and 12 and then decreased (p < 0.05) on Day 15. Mean concentration of mRNA for 3 beta-HSD was not different (p = 0.33) in luteal tissue on any day examined. In small luteal cells, mean concentrations of mRNA for 3 beta-HSD decreased between Days 6 and 15 (p < 0.05) while in large luteal cells, mean concentrations decreased (p < 0.05) between Days 12 and 15. (ABSTRACT TRUNCATED AT 250 WORDS)
Microbiological Evaluation of the New VITEK 2 Neisseria-Haemophilus Identification Card

ABSTRACT VITEK 2 is an automated identification system for diverse bacterial and fungal species. A new card (the Neisseria-Haemophilus [NH] card) for the identification of Neisseria spp. , Haemophilus spp. , and other fastidious gram-negative or gram-variable microorganisms has been developed, but its performance in a routine clinical laboratory has not yet been evaluated. In this study, a total of 188 bacterial strains belonging to the genera Actinobacillus, Campylobacter, Capnocytophaga, Cardiobacterium, Eikenella, Gardnerella, Haemophilus, Kingella, Moraxella, and Neisseria were investigated. The NH card was able to identify 171 strains (91%) correctly without the need for extra tests; one strain (0.5%) was misidentified, and five strains (2.7%) could not be classified. Eleven strains (5.8%) were identified with a low level of discrimination, and simple additional tests were required to increase the correct-identification rate to 96.8%. The results were available within 6 h. Based on these results, the new VITEK 2 NH card appears to be a good method for the identification of diverse groups of fastidious organisms, which would otherwise require testing with multiple systems. However, more work is needed to evaluate the performance of VITEK 2 with regard to Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella bacteria because of the insufficient number of strains tested in this study. Moreover, further reduction of the detection time would be desirable.
Evaluation of retinal nerve fiber layer progression in glaucoma: a study on optical coherence tomography guided progression analysis.

PURPOSE To evaluate optical coherence tomography (OCT) retinal nerve fiber layer thickness (RNFLT) measurement for glaucoma progression analysis. METHODS One hundred sixteen eyes of 64 patients with glaucoma who were observed within a period of 5 years were included. All eyes had at least four serial RNFL measurements obtained with the Stratus OCT (Carl Zeiss Meditec, Dublin, CA) and with the first and last measurements separated by at least 3 years. Visual field (VF) testing was performed on the same day as RNFL imaging. Serial average RNFLTs were evaluated with guided progression analysis (GPA). VF progression was assessed with trend analysis of the visual field index (VFI). Factors associated with the rate of change in RNFLT were examined with a linear mixed model. RESULTS A total of 1101 OCT scans and 1029 VFs were analyzed. Twenty-one and 22 eyes had progression according to RNFL and VF measurements, respectively, and 3 eyes had progression according to both measurements. The rate of change in VFI and RNFLT ranged between -0.5% and -7.2% per year (median loss, -3.0%/y) and between -1.2 and -15.4 microm per year (median loss, -3.3 microm/y), respectively. The sector at seven o'clock (right eye orientation) was the most frequent location that showed progression. A greater baseline RNFLT was associated with an increased rate of reduction of RNFLT (P = 0.034). CONCLUSIONS OCT GPA offers a new approach to augment glaucoma progression analysis. The rate of RNFLT thinning was variable among patients with glaucoma, with an increased rate of loss in patients with a higher baseline RNFLT.
Galectin-8 Induces Apoptosis in Jurkat T Cells by Phosphatidic Acid-mediated ERK1/2 Activation Supported by Protein Kinase A Down-regulation*

Galectins have been implicated in T cell homeostasis playing complementary pro-apoptotic roles. Here we show that galectin-8 (Gal-8) is a potent pro-apoptotic agent in Jurkat T cells inducing a complex phospholipase D/phosphatidic acid signaling pathway that has not been reported for any galectin before. Gal-8 increases phosphatidic signaling, which enhances the activity of both ERK1/2 and type 4 phosphodiesterases (PDE4), with a subsequent decrease in basal protein kinase A activity. Strikingly, rolipram inhibition of PDE4 decreases ERK1/2 activity. Thus Gal-8-induced PDE4 activation releases a negative influence of cAMP/protein kinase A on ERK1/2. The resulting strong ERK1/2 activation leads to expression of the death factor Fas ligand and caspase-mediated apoptosis. Several conditions that decrease ERK1/2 activity also decrease apoptosis, such as anti-Fas ligand blocking antibodies. In addition, experiments with freshly isolated human peripheral blood mononuclear cells, previously stimulated with anti-CD3 and anti-CD28, show that Gal-8 is pro-apoptotic on activated T cells, most likely on a subpopulation of them. Anti-Gal-8 autoantibodies from patients with systemic lupus erythematosus block the apoptotic effect of Gal-8. These results implicate Gal-8 as a novel T cell suppressive factor, which can be counterbalanced by function-blocking autoantibodies in autoimmunity.
Computed Tomography Versus Water-Soluble Contrast Swallow in the Detection of Intrathoracic Anastomotic Leak Complicating Esophagogastrectomy (Ivor Lewis): A Prospective Study in 97 Patients

Purpose:Water-soluble contrast swallow (CS) is usually performed before refeeding for anastomosis assessment after esophagectomy with intrathoracic anastomosis but the sensitivity of CS is low. Another diagnostic approach is based on analysis of computed tomography (CT) scan with oral contrast and of CT mediastinal air images. We undertook to compare them prospectively. Methods:Ninety-seven patients with an esophageal carcinoma operated by intrathoracic anastomosis were included prospectively in a study based on a CT scan at postoperative day 3 (without oral and intravenous contrast) and CT scan and CS at day 7. CT scan analysis consisted of assessing contrast and air leakage. In case of doubt, an endoscopy was done. Results:A diagnosis of anastomotic leak was made in 13 patients (13.4%), in 2 cases before day 7 and in 3 beyond day 7. At day 3, 94 CT scans were performed, but the diagnostic value was poor. In 95 patients with both CS and CT scan at day 7, CS disclosed a leak in 5 of 11, and CT scan was abnormal in 8 of 11. Leakage of contrast and/or presence of mediastinal gas had the best negative predictive value (95.8%). Endoscopy was done in 16 patients with only mediastinal gas at day 7 CT scan. It disclosed a normal anastomosis in 11, fibrin deposits in 4, and a leak in 1. Conclusions:In comparison with CS only, CT at day 7 improves the sensitivity and negative predictive value for diagnosing an anastomotic leak. In case of doubt endoscopy is advisable. This approach provides an accurate assessment of the anastomosis before refeeding.
Expression of CD44s, vascular endothelial growth factor, matrix metalloproteinase-2 and Ki-67 in peritoneal, rectovaginal and ovarian endometriosis.

OBJECTIVE To understand the pathogenetic mechanisms of endometriosis by examining the expression of adhesion molecules (CD44s), angiogenic factor (VEGF) and matrix protease and to perform Ki-67 labeling for evaluation of proliferative activity. STUDY DESIGN Twenty-nine peritoneal endometriosis lesions (9 red, 12 black and 8 white), 11 rectovaginal and 22 ovarian were obtained. Immunohistochemical staining was performed with antibodies for CD44, VEGF, MMP-2 and Ki-67. RESULTS CD44s were expressed mainly in stroma and showed higher expression in glandular epithelium of peritoneal endometriosis than in rectovaginal and ovarian endometriosis. The stroma in red and white lesions showed higher MMP-2 expression than in black lesions. The stromal cells in rectovaginal endometriosis showed significantly lower expression of Ki-67 (p = 0.002) than in peritoneal and ovarian endometriosis. When endometriosis was analyzed according to the revised American Fertility Society classification, Ki-67 expression was high in glandular epithelium in stages I and II (p = 0.025), whereas MMP-2 expression in stromal cells was significantly high (p < 0.001) in stages III and IV. CONCLUSION CD44, VEGF and MMP-2 were consistently expressed in endometriotic epithelial and stromal cells. White lesions of peritoneal endometriosis should not be regarded as an inactive state, and MMP-2 in stromal cells may be responsible for the progression of endometriosis. The macroscopic appearance of endometriotic lesions should not be used as a criterion to define the degree of activity.
“Dude, I’m Seventeen … It’s Okay Not to Have Sex by This Age”: Feelings, Reasons, Pressures, and Intentions Reported by Adolescents Who Have Not Had Sexual Intercourse

This study investigated feelings, reasons, pressures, and previous sexual experiences reported by students who have not had sexual intercourse and how these factors are associated with self-rated likelihood of having sex during the next year. Using data from the Fifth National Survey of Australian Secondary Students and Sexual Health (n = 783), this study found, in general, students had positive feelings about not having sex. Reasons for not having sex such as being proud to say no and not being ready were rated higher in importance than fear of potential outcomes or religious/cultural beliefs. Students reported limited pressure from parents and friends and, despite not having sexual intercourse, more than half of the sample had experienced some form of sexual activity. Stronger likelihoods of having sex during the next year were reported by students who had previously engaged in other sexual practices, reported more pressure from friends to have sex, and had negative feelings about not having sex. Sexuality educators can use these findings to guide approaches to sex education, emphasizing feelings, intentions, and reasoning over fear tactics. Discussion of a range of sexual practices will address more closely the experiences of young students as they begin their sexual lives.
Gestational trophoblastic disease: the significance of vaginal metastases.

PURPOSE To evaluate patients with vaginal lesions in gestational trophoblastic disease and determine prognostic and therapeutic implications applicable to management. METHODS Twelve patients among 75 cases of gestational trophoblastic neoplasia were analyzed retrospectively between 1990 and 1997. Vaginal metastases were documented by physical examination and biopsy. Two patients received MAC III regimen (5 and 7 courses), 4 patients received EMA-CO regimen for 2 to 11 courses, while 6 were administered methotrexate alone. Remission was defined as 3 weekly beta hCG levels below assay sensitivity (<5 mIU/ml). RESULTS The mean age of the patients was 25.4 years. While 10 of the patients presented initially with hemorrhage and bloody leukorrhea, the remaining 2 were diagnosed during a routine study of hydatidiform mole. The sites of involvement were almost always the anterior distal vaginal wall. Five cases had additional lung and 1 case had lung, liver, spleen and brain metastases. Three of the patients who received methotrexate as monotherapy did not respond to therapy and were switched to EMA-CO. Overall survival was 91.6%. One patient died in the first month of the initial therapy. CONCLUSION The presence of large vaginal metastases should be classified as a high-risk factor and these patients must be treated by multiple agent chemotherapy.
The role of mucosal immunity and host genetics in defining intestinal commensal bacteria

Purpose of review Dramatic advances in molecular characterization of the largely noncultivable enteric microbiota have facilitated better understanding of the composition of this complex ecosystem at broad phylogenetic levels. This review outlines current understanding of mechanisms by which commensal bacteria are controlled and shaped into functional communities by innate and adaptive immune responses, antimicrobial peptides produced by epithelial cells and host genetic factors. Recent findings Secretory IgA, which targets enteric bacteria, regulates the number, composition, and function of luminal bacteria. Likewise, epithelial production of antimicrobial peptides helps control enteric microbiota growth, translocation, and perhaps composition. The developing role of innate signaling pathways, such as Toll-like receptors and NOD2, is beginning to be studied, with dysbiosis following their genetic deletion. Inflammation and effector immune responses lead to decreased diversity and selective alterations of functionally active bacterial species such as Escherichia coli and Faecalibacterium prausnitzii that have proinflammatory and protective activities, respectively. Studies of humans, mice, and comparative species indicate that both genetic and early environmental factors influence the development of a stable intestinal microbiota. Summary Genetic and mucosal immunity strongly influence the composition and function of enteric commensal bacteria. This understanding should help develop strategies to correct dysfunctional altered microbiota in genetically susceptible individuals, better diagnose and correct potential dysbiosis in high-risk individuals at a preclinical stage, and therapeutically target pathogenic bacterial species that help drive chronic inflammatory conditions.
Simvastatin inhibits growth via apoptosis and the induction of cell cycle arrest in human melanoma cells

Competitive inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase (the statins) that inhibit the synthesis of mevalonic acid are in wide use for treatment of hypercholesterolemia. Although antitumor effects on a variety of cell types have been reported for statins, the effect of simvastatin (one of the statins) on human melanoma cell lines is not known. Here, we report antitumor effects of simvastatin on human melanoma cell lines. We treated human melanoma cell lines, A375M, G361, C8161, GAK, and MMAc with simvastatin in various concentrations for 1 to 3 days. To investigate the antitumor effect of simvastatin, we analyzed cell viability, morphologic changes, reversibility of inhibition by geranylgeranyl pyrophosphate and farnesyl pyrophosphate, apoptosis and the cell cycle. Simvastatin treatment reduced cell viability in all five melanoma cell lines. The different melanoma cell lines, however, displayed different sensitivities to simvastatin. The addition of geranylgeranyl pyrophosphate to A375M and G361 cells in the presence of simvastatin completely restored the viability of cells, but the addition of farnesyl pyrophosphate did not. DNA fragmentation assay showed that simvastatin induced apoptosis in A375M and G361 cells. Simvastatin caused a G1 arrest in G361 and MMAc cells. Consistent with the cell cycle arrest, simvastatin caused an increase in the mRNA levels of p21 and p27 on G361 and MMAc cells.We conclude that simvastatin has an antitumor effect on human melanoma cells in vitro via apoptosis and cell cycle arrest. These results suggest that simvastatin may be an effective anticancer drug for malignant melanoma.
PULMONARY TOXICITY OF SIMULATED LUNAR AND MARTIAN DUSTS IN MICE: I. HISTOPATHOLOGY 7 AND 90 DAYS AFTER INTRATRACHEAL INSTILLATION

NASA is contemplating sending humans to Mars and to the moon for further exploration. Volcanic ashes from Arizona and Hawaii with mineral properties similar to those of lunar and Martian soils, respectively, are used to simulate lunar and Martian environments for instrument testing. Martian soil is highly oxidative; this property is not found in Earth's volcanic ashes. NASA is concerned about the health risk from potential exposure of workers in the test facilities. Fine lunar soil simulant (LSS), Martian soil simulant (MSS), titanium dioxide, or quartz in saline was intratracheally instilled into groups of 4 mice (C57BL/6J) at 0.1 mg/mouse (low dose, LD) or 1 mg/mouse (high dose, HD). Separate groups of mice were exposed to ozone (0.5 ppm for 3 h) prior to MSS instillation. Lungs were harvested for histopathological examination 7 or 90 days after the single dust treatment. The lungs of the LSS-LD groups showed no evidence of inflammation, edema, or fibrosis; clumps of particles and an increased number of macrophages were visible after 7 days but not 90 days. In the LSS-HD-7d group, the lungs showed mild to moderate alveolitis, and perivascular and peribronchiolar inflammation. The LSS-HD-90d group showed signs of mild chronic pulmonary inflammation, septal thickening, and some fibrosis. Foci of particle-laden macrophages (PLMs) were still visible. Lung lesions in the MSS-LD-7d group were similar to those observed in the LSS-HD-7d group. The MSS-LD-90d group had PLMs and scattered foci of mild fibrosis in the lungs. The MSS-HD-7d group showed large foci of PLMs, intra-alveolar debris, mild-to-moderate focal alveolitis, and perivascular and peribronchiolar inflammation. The MSS-HD-90d group showed focal chronic mild-to-moderate alveolitis and fibrosis. The findings in the O 3 -MSS-HD-90d group included widespread intra-alveolar debris, focal moderate alveolitis, and fibrosis. Lung lesions in the MSS groups were more severe with the ozone pretreatment. The effects of O 3 and MSS coexposure appeared to be more than additive. Results for the TiO 2 and quartz controls were consistent with the known pulmonary toxicity of these compounds. The overall severity of lung injury was TiO 2 < LSS < MSS < O 3 + MSS < quartz. Except for TiO 2, the increased duration of dust presence in the lung from 7 to 90 days transformed the acute inflammatory response to a chronic inflammatory lesion. This study showed that LSS and MSS are more hazardous in the lungs than nuisance dusts.
Public perception of a range of potential food risks in the United Kingdom

This study aimed to use a standard questionnaire to obtain a nationally representative sample of opinions on a range of potential food risks. Participants were a national sample of 1182 subjects selected using three different approaches: random and sentinel postal samples and a telephone survey. A modified psychometric questionnaire (the Perceived Food Risk Index) was administered to subjects on three occasions, spanning five time-points. Baseline data collection was undertaken from October to December 1998 (phase 1). The second wave of data collection was undertaken over three time-points in February, April and July 1999 (one-third of respondents to phase 1 at each time-point - data combined as phase 2), and the final phase of data collection was between October and December 1999 (phase 3). Principal components analysis was used to assess the intercorrelations between the items on the questionnaire. Two main components were identified as 'dread' and 'knowledge'. Saturated fats were perceived as the least dreaded and the most known of the potential risks considered, while bovine spongiform encephalopathy and Salmonella were the risks dreaded the most. There was a slight perception that the potential risks had become more known over the year, especially for growth hormones. This study has raised a number of important issues for risk communicators. Despite current policy aimed at reducing fat intake, this will be difficult to achieve at a population level since people are not worried about its impact, yet food safety continues to be a significant concern to the public.
Analytic determination of the resolution-equivalent effective diameter of a pinhole collimator

To account for photon penetration, the formulas used to calculate the geometric resolution of a pinhole collimator use an equivalent diameter d/sub e/ rather than the physical diameter of the aperture. The expression commonly used for d/sub e/, however, was originally derived to account for penetration in sensitivity calculations. In this paper, we show that the concept of equivalent diameter is also applicable to resolution calculations, propose angular-dependent expressions for d/sub e/ specific to resolution calculations, and discuss the limits of their applicability and how they compare to other expressions. Results show that for normal incidence Paix's expression for d/sub e/ tends to overestimate the resolution-equivalent diameter for full-width-at-half-maximum resolution, whereas Anger's is a better approximation, but may produce underestimates for submillimeter resolution imagers, especially in the case of high-energy photons. For grazing incidence, both expressions may result in significant overestimates.
Role of dopaminergic gene polymorphisms (DBH 19 bp indel and DRD2 Nco I) in genetic susceptibility to migraine in North Indian population.

OBJECTIVE The dopaminergic system plays a major role in migraine. We aimed to look for association of polymorphisms in dopaminergic genes in genetic susceptibility to migraine in North Indian population. In the present study, two polymorphisms, DBH 19 bp indel (rs no. 72393728) and DRD2 Nco I (rs no. 6275), were selected. DESIGN Using case-control design, 301 migraine patients (202 migraine without aura and 99 migraine with aura) and 202 healthy controls were recruited in the study. Genotyping was done using polymerase chain reaction (PCR) (DBH 19 bp indel) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (DRD2 Nco I). Logistic regression analysis was used to find the contribution of these polymorphisms in migraine. Bonferroni's correction was applied for multiple comparisons. RESULTS In DBH 19 bp indel polymorphism, a significant association was observed at allelic level (P = 0.027) especially in females (P = 0.016) on comparing migraine patients with healthy controls. In DRD2 Nco I polymorphism, no significant association was observed in migraine patients or any subgroups. CONCLUSION Our study suggests a potentially significant role of DBH 19 bp indel polymorphism in migraine susceptibility.