Effect of beta-glucan in preventing bacterial translocation in a model of experimental obstructive jaundice

Abstract Aim. Sepsis is the major cause of post-operative morbidity and mortality in obstructive jaundice as a result of bacterial translocation from the gut. This study was conducted to investigate the effect of beta-glucan in preventing bacterial translocation in an animal model where obstructive jaundice was developed by common bile duct ligation. Methods. Forty-five Wistar-albino rats were divided into three groups of fifteen animal each. Only laparotomy was administered to the firs t group. Bile duct ligation was administered to the seco nd group. Bile duct ligation and oral beta-glucan for ten days were administered to the thir d group. The animals were sacrified at the end of the tent h day. Blood, liver, spleen and mesenteric lymph nodes were cultured. The samples taken from terminal ileum and liver were examined histopathologically. Aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total bilirubin, direct bilirubin and C-reactive protein (CRP) analyses were done on the blood samples taken from the rats. Results. In the first group bacterial translocation was observed in one animal whereas bacterial translocation was observed in twelve animals in II’nd group. In thir d group bacterial translocation was determined in six animals that received beta-glucan. The differences between these three groups were statistically significant (p 0.05). Conclusion. The result of this study showed that beta-glucan, as a natural immune system activator, has an effect in preventing bacterial translocation in obstructive jaundice. Keywords: Obstructive jaundice, bacterial translocation, beta-glucan Ozet Amac. Sepsis, barsaktan kaynaklanan bakteriyel translokasyonun bir sonucu olarak tikanma sariliginda post-operatif morbidite ve mortalitenin en onemli sebebidir. Bu calisma, ortak safra kanali ligasyonu yoluyla tikanma sariligi gelistirilen bir hayvan modelinde bakteriyel translokasyonun onlenmesinde beta-glukanin etkisi arastirmak icin yapilmistir. Yontem. Kirk bes adet Wister albino rat, her birinde onbes hayvanin bulundugu uc gruba ayrildi. I.gruba sadece laparatomi yapildi. II. gruba safra kanali ligasyonu yapildi. III. gruba ise safra kanali ligasyonu ve on gun beta-glukan oral verildi. Onuncu gunun sonunda hayvanlar sakrifiye edildi. Kan, karaciger, dalak ve mezenter lenf nodu kulturleri ekildi. Terminal ileum ve karacigerden alinan ornekler histopatolojik olarak incelendi. Ratlardan alinan kan orneklerinden aspartate aminotransferaz (AST), alanine aminotransferaz (ALT), alkalin fosfatase (ALP), laktat dehidrogenaz (LDH), total bilirubin, direk Bilirubin ve C-reaktif protein (CRP) calisildi. Bulgular. Birincigrupta 1 hayvanda bakteriyel translokasyon gozlenirken, II. grupta bakteriyel translokasyon oniki hayvanda gozlendi. III. grupta beta-glukan verilen hayvanlarin alti tanesinde bakteriyel translokasyon tespit edildi. Bu uc grup arasindaki fark istatistiki acidan anlamliydi (p 0.05). Sonuc. Elde edilen sonuclar gostermistir ki, tikanma sariliginda gelisen bakteriyel translokasyonu onlemede dogal bir immun sistem aktivatoru olan beta glukanin etkinligi vardir. Anahtar sozcukler: Tikanma sariligi, beta-glukan, bakteriyal translokasyonu