Miracle-dz/UniFAR-FTUs huggingface dataset:
Determination of Chemical Components of Benzene/Ethanol Extractive of Beech Wood by GC/MS

Beech tree has high adaptability to environment and tolerance to water stress, and a long history of utilization and plantation in many countries. Moreover, beech wood is world- widely used as high-grade timber of paper making for its dendrological characteristics of fast growing, high biomass, medium density, high impact ductility, compressively bend strength. However, researches on Beech wood were mostly focused on the dendrological analyses and utilizations of its wood, especially papermaking, and lacked those to analyze the chemical components of extractive of Beech wood, which was very important to recover and utilize the polluting wastewater from pretreated Beech wood. Therefore, in order to separate top value-added bioactive components from wastewater of treated Beech wood and hence decrease its pollution to water environment, we attempted to analyze the chemical components of benzene/ethanol extractive of Beech wood by means of GC/MS. Relative content of each component was determined by area normalization, and 16 compounds representing 83.4% of the extractives were identified. The most abundant constituents were as: 1,2-benzenedicarboxylic acid,4-hydroxy- (43.53%), 6-(2- formylhydrazino)-N,N'-bis(isopropyl)-1,3,5-triazine-2,4-diamine (9.50%), benzofuran,2,3-dihydro- (6.08%), 4-morpholineethanol, (5.11%), p-xylene (4.94%), cyclohexane,1,1'-oxybis- (3.35%), phenol,4-(3-hydroxy-1-propenyl)-2-methoxy- (3.15%), phenol,2,6-dimethoxy- (3.13%), 1-hexanol,2-ethyl- (3.13%), bicyclo[4.1. 0]heptan-2-one, 6-methyl- (2.00%), etc. As the first report here, our result by GC/MS showed that the benzene- methanol extractive of freeze-dried beech wood can be developed into top value-added materials of medicines, cosmetics, biofuel, and industrial solvents.
Expression of functional Candida antarctica lipase B in a cell‐free protein synthesis system derived from Escherichia coli

This article reports the cell‐free expression of functional Lipase B from Candida antarctica (CalB) in an Escherichia coli extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led to the soluble expression of CalB. In addition, the functional enzyme was generated by applying the optimal redox potential. When examined using p‐nitrophenyl palmitate as a substrate, the specific activity of the cell‐free synthesized CalB was higher than that of the reference protein produced in Pichia pastoris. These results highlight the potential of cell‐free protein synthesis technology as a powerful platform for the rapid expression, screening and analysis of industrially important enzymes. © 2009 American Institute of Chemical Engineers Biotechnol. Prog. , 2009
Melatonin‐induced gene expression changes and its preventive effects on adriamycin‐induced lipid peroxidation in rat liver

Abstract: Adriamycin (ADR) provokes lipid peroxidation process, while melatonin (MEL) is a free radical scavenger that has been found to protect against lipid peroxidation in vitro and in many experimental models. In the present study, the effects of ADR and the combination of ADR and MEL were analyzed on the modulation of fatty acid composition, lipid peroxidation and gene expression in rat liver. Sixty genes were selected for the study of relative gene expression changes in the liver. ADR treatment decreased the polyunsaturated fatty acids C22:6 n‐3 and C20:4 n‐6 in rat liver mitochondria. When the treatment of ADR was followed by MEL, decrease in these fatty acids could not be detected. A significant increase in lipid peroxidation was observed after administration of ADR, which was restored to control values by post‐treatment with MEL. Gene expression profiles of ADR‐ versus ADR + MEL‐treated rat livers indicated that both treatments induced significant changes. Quantitative real‐time polymerase chain reaction analysis of 60 genes involved in oxidative stress revealed that cyp1b1, which is involved in electron transport, cyclin‐dependent kinase inhibitor 1a that possesses cyclin‐dependent protein kinase inhibitor activity, was induced at a more pronounced level in the ADR + MEL‐treated samples than in the ADR‐treated ones. Several genes having roles in heat‐shock response were downregulated in MEL‐treated animals, such as hsp40, hsp70 and hsp90 proteins reflecting the reduced oxidative stress in these animals. Global gene expression analysis will highlight the gene expression changes accompanying oxidative damage and its prevention in more details.
Chromium (VI) induces insulin resistance in 3T3-L1 adipocytes through elevated reactive oxygen species generation

Reactive oxygen species (ROS) have been proposed to be involved in the development of insulin resistance, although the exact molecular link between ROS and insulin resistance remains to be determined. Chromium (Cr(VI)) is known as an inducer of ROS. Therefore, this study examined whether Cr(VI) could induce insulin resistance. It demonstrated that Cr(VI) treatment significantly inhibited insulin-stimulated glucose uptake and attenuated insulin signalling. Moreover, Cr(VI) treatment markedly increased the intracellular levels of superoxide anion, hydrogen peroxide and hydroxyl radical. N-acetylcysteine, superoxide dismutase and catalase can block the ROS generation and alleviate the insulin resistance induced by Cr(VI) treatment. In addition, Cr(VI) treatment induced endoplasmic reticulum (ER) stress and JNK activation and these effects were diminished by N-acetylcysteine. These results suggested that ROS generation through Cr(VI) treatment cause ER stress, JNK activation and insulin resistance in adipocytes. Therefore, the oxidative stress could be a potential interventional target for insulin-resistance related diseases.
The effect of microablative fractional CO2 laser on vaginal flora of postmenopausal women

Abstract Objectives: To assess the effect of microablative fractional CO2 laser (MFCO2-Laser) therapy on the vaginal microenvironment of postmenopausal women. Methods: Three laser therapies at monthly intervals were applied in postmenopausal women with moderate to severe symptoms of genitourinary syndrome of menopause, pH of vaginal fluid >4.5 and superficial epithelial cells on vaginal smear <5%. Vaginal fluid pH values, fresh wet mount microscopy, Gram stain and aerobic and anaerobic cultures were evaluated at baseline and 1 month after each subsequent therapy. Nugent score and Hay-Ison criteria were used to evaluate vaginal flora. Results: Fifty-three women (mean age 57.2 ± 5.4 years) participated and completed this study. MFCO2-Laser therapy increased Lactobacillus (p < 0.001) and normal flora (p < 0.001) after the completion of the therapeutic protocol, which decreased vaginal pH from a mean of 5.5 ± 0.8 (initial value) to 4.7 ± 0.5 (p < 0.001). The prevalence of Lactobacillus changed from 30% initially to 79% after the last treatment. Clinical signs and symptoms of bacterial vaginosis, aerobic vaginitis or candidiasis did not appear in any participant. Conclusion: MFCO2-Laser therapy is a promising treatment for improving the vaginal health of postmenopausal women by helping repopulate the vagina with normally existing Lactobacillus species and reconstituting the normal flora to premenopausal status.
Correlates of improvement in substance abuse among dually diagnosed veterans with post-traumatic stress disorder in specialized intensive VA treatment.

Substantial rates of substance use comorbidity have been observed among veterans with Post-Traumatic Stress Disorder (PTSD), highlighting the need to identify patient and program characteristics associated with improved outcomes for substance abuse. Data were drawn from 12,270 dually diagnosed veterans who sought treatment from specialized intensive Veterans Health Administration PTSD programs between 1993 and 2011. The magnitude of the improvement in Addiction Severity Index (ASI) alcohol and drug use composite scores from baseline was moderate, with effect sizes (ES) of -. 269 and -. 287, respectively. Multivariate analyses revealed that treatment in longer-term programs, being prescribed psychiatric medication, and planned participation in reunions were all associated with slightly improved outcomes. Reductions in substance use measures were associated with robust improvements in PTSD symptoms and violent behavior. These findings suggest not only synergistic treatment effects linking improvement in PTSD symptoms with substance use disorders among dually diagnosed veterans with PTSD, but also to reductions in violent behavior. Furthermore, the findings indicate that proper discharge planning in addition to intensity and duration of treatment for dually diagnosed veterans with severe PTSD may result in better outcomes. Further dissemination of evidence-based substance abuse treatment may benefit this population.
Whole‐cell recording from honeybee olfactory receptor neurons: ionic currents, membrane excitability and odourant response in developing workerbee and drone

Whole‐cell recording techniques were used to characterize ionic membrane currents and odourant responses in honeybee olfactory receptor neurons (ORNs) in primary cell culture. ORNs of workerbee (female) and drone (male) were isolated at an early stage of development before sensory axons connect to their target in the antennal lobe. The results collectively indicate that honeybee ORNs have electrical properties similar, but not necessarily identical to, those currently envisaged for ORNs of other species. Under voltage clamp at least four ionic currents could be distinguished. Inward currents were made of a fast transient, tetrodotoxin‐sensitive sodium current. In some ORNs a cadmium‐sensitive calcium current was detected. ORNs showed heterogeneity in their outward currents: either outward currents were made of a delayed rectifier type potassium current, which was partially blocked by tetraethyl ammonium or quinidine, or were composed of a delayed rectifier type and a transient calcium‐dependent potassium current, which was cadmium‐sensitive and abolished by removal of external calcium. The proportion of each of the two outward currents, however, was different within the ORNs of the two sexes suggesting a gender‐specific functional heterogeneity. ORNs showed heterogeneity in action potential firing properties: depolarizing current steps elicited either one action potential or, as in most of the cells, it led to repetitive spiking. Action potentials were tetrodotoxin‐sensitive suggesting they are carried by sodium. Odourant stimulation with different mixtures and pure substances evoked depolarizing receptor potentials with superimposed action potentials when spike threshold was reached. In summary, honeybee ORNs are remarkably mature at early stages in their development.
Effects of short-term glucocorticoids on cardiovascular biomarkers.

CONTEXT Glucocorticoids are known to acutely increase blood pressure, suppress inflammation, and precipitate insulin resistance. However, the short-term effects of glucocorticoids on other cardiovascular risk factors remain incompletely characterized. OBJECTIVE Our objective was to determine the effects of a short course of dexamethasone on multiple cardiovascular biomarkers and to determine whether suppression of morning cortisol in response to low-dose dexamethasone is correlated with cardiovascular risk markers in healthy volunteers. DESIGN We conducted a randomized, double-blind, placebo-controlled study. SETTING The study took place in a tertiary care hospital. STUDY SUBJECTS Twenty-five healthy male volunteers, ages 19-39 yr, participated in the study. INTERVENTION Subjects received either 3 mg dexamethasone twice daily or placebo for 5 d. Subjects also underwent a low-dose (0.5 mg) overnight dexamethasone suppression test. MEASURES Parameters examined before and after the 5-d intervention included heart rate, blood pressure, weight, fasting lipid variables, homocysteine, renin, aldosterone, insulin resistance (homeostasis model assessment), high-sensitivity C-reactive protein, B-type natriuretic peptide, flow-mediated and nitroglycerin-mediated brachial artery dilatation, and heart rate recovery after exercise. All measurements were done in the morning hours in the fasting state. RESULTS Dexamethasone increased systolic blood pressure, weight, B-type natriuretic peptide, and high-density-lipoprotein-cholesterol. Dexamethasone decreased resting heart rate, high-sensitivity C-reactive protein, and aldosterone and tended to attenuate nitroglycerin-mediated vasodilatation. There was no effect on flow-mediated vasodilatation, diastolic blood pressure, triglycerides, low-density-lipoprotein-cholesterol, nonesterified fatty acids, homocysteine, or heart rate recovery. The response of circulating cortisol to low-dose dexamethasone had no significant correlation with any of the cardiovascular risk markers. CONCLUSIONS Short-term glucocorticoids elicits both favorable and unfavorable effects on different cardiovascular risk factors. Manipulation of specific glucocorticoid-responsive physiological pathways deserves further study.
Changes in Anterior and Posterior Corneal Parameters in Patients with Keratoconus After Intrastromal Corneal-ring Segment Implantation

Abstract Purpose: To compare changes in the anterior and the posterior corneal parameters and the anterior chamber depth (ACD) measured by a rotating Scheimpflug camera and scanning-slit topography before and after intrastromal corneal ring segment (ICRS) implantation. Methods: Twenty-five eyes of 21 keratoconus patients who had Intacs (Addition Technology, Sunnyvale, CA) ICRS implantation were included. The anterior segment parameters of the participants were measured before and 3 months after Intacs ICRS implantation with a rotating Scheimpflug camera and scanning-slit topography. Outcome measures included uncorrected (UDVA) and corrected (CDVA) distance visual acuities, refraction and corneal topography. Results: The UDVA improved in 21 eyes (84%) and CDVA in 18 eyes (72%). The anterior average keratometry decreased from 51.80 D preoperatively to 49.59 D postoperatively (p < 0.001) using the rotating Scheimpflug camera and Sim K decreased from 51.21 to 47.44 D using the scanning-slit topography. The posterior average keratometry decreased from −8.03 D preoperatively to −8.42 D postoperatively (p < 0.001) using the rotating Scheimpflug camera. ACD decreased from 3.44 to 3.32 mm (p < 0.001) and from 3.28 to 3.19 mm (p < 0.001) using the rotating Scheimpflug camera and scanning-slit topography, respectively. Conclusions: The implantation of Intacs segments induced corneal flattening of the anterior surface and corneal steepening of the posterior surface. Both anterior corneal flattening and posterior corneal steepening contributed to the decrease in the keratometric power.
Spatial Variation and Factors Impacting Grassland Circulation Price in Inner Mongolia, China

As the circulation of grassland use rights in China increases, relatively little is known about the factors that influence circulation price. This paper examines the spatial distribution of grassland circulation prices and the impact of various attributes on grassland circulation prices in Inner Mongolia Autonomous Region (IMAR). Spatial autocorrelation tests and quantile regression methods are applied to data from an online land-circulation website covering the period from January to October 2017. The spatial analysis found that grassland circulation price does vary greatly throughout IMAR but that no significant spatial autocorrelation is evident. The quantile regression analysis revealed significant, though varied, quantile effects across the price distribution indicating that local market structures, strong demand for grazing land in desert steppe, high demand of poor herders for smaller plots, and high demand of richer herders for larger plots all play an important role in determining circulation prices. These nuanced findings should enable policy makers, grassland users, and other grassland actors to better understand how grassland price is determined with respect to a range of factors across the quantiles of price as well as the spatial pattern of price characteristics. This information and understanding are a crucial step in improving grassland circulation.
Efficient Virus-Induced Gene Silencing in Arabidopsis1

Virus-induced gene silencing (VIGS) is a plant RNA-silencing technique that uses viral vectors carrying a fragment of a gene of interest to generate double-stranded RNA, which initiates the silencing of the target gene. Several viral vectors have been developed for VIGS and they have been successfully used in reverse genetics studies of a variety of processes occurring in plants. This approach has not been widely adopted for the model dicotyledonous species Arabidopsis (Arabidopsis thaliana), possibly because, until now, there has been no easy protocol for effective VIGS in this species. Here, we show that a widely used tobacco rattle virus-based VIGS vector can be used for silencing genes in Arabidopsis ecotype Columbia-0. The protocol involves agroinfiltration of VIGS vectors carrying fragments of genes of interest into seedlings at the two- to three-leaf stage and requires minimal modification of existing protocols for VIGS with tobacco rattle virus vectors in other species like Nicotiana benthamiana and tomato (Lycopersicon esculentum). The method described here gives efficient silencing in Arabidopsis ecotype Columbia-0. We show that VIGS can be used to silence genes involved in general metabolism and defense and it is also effective at knocking down expression of highly expressed transgenes. A marker system to monitor the progress and efficiency of VIGS is also described.
Comparison of Two Preterai Formulas With or Without Addition of Medium‐Chain Triglycerides (MCTs). II: Effects on Mineral Balance

Medium-chain triglycerides (MCTs) are included in the fat blend of several preterm formulas because of their complete absorption and rapid oxidation. The effects of two different fat blend compositions on calcium (Ca), phosphorus (P), and magnesium (Mg) balances and plasma levels and on plasma levels of parathyroid hormone (PTH), alkaline phosphatase (AP), and 1,25-dihydroxyvitamin D [1,25-(OH)2D] were investigated in 28 healthy very-low-birth weight infants at 4 weeks of age. A preterm formula with a traditional corn oil/MCT blend containing 38% MCTs (MCT group) was compared to a new fat blend, designed to resemble human milk more, containing 6% MCTs (LCT group). There was a higher absorption of Ca in the MCT group (73% vs. 60%. p < 0.005), and an equal absorption of P (both 92%). The excretion of Ca correlated with the excretion of fat (p < 0.00005). The LCT group showed a higher median PTH level (MCT: 2.1 pmol/L, LCT: 4.7 pmol/L, p < 0.01) and a higher urinary P excretion (p < 0.001). Mg absorption was also lower with LCT, but retention of Mg exceeded intrauterine values in both groups. Mineral plasma levels were in the normal range in both groups. AP was not different between groups and in the upper part of the reference range, whereas 1,25-(OH)2D levels were above the normal range and also not different between groups. We conclude that with the LCT formula, Ca absorption is slightly lower than with the MCT formula, whereas P absorption is unaffected. The resulting imbalance in absorption is compensated for by an increased urinary excretion of P. We conclude that current recommendations for the maximum Ca/P ratio in preterm formulas (2:1) might be too low in formulas containing only LCT.
Expression of interleukin 1 alpha, interleukin 1 beta and interleukin 1 receptor antagonist mRNA in mouse brain: regulation by bacterial lipopolysaccharide (LPS) treatment.

Lipopolysaccharide (LPS) stimulation is known to induce interleukin-1 (IL-1) mRNA expression in various immune cell types. Since IL-1 synthesis has been suggested to occur locally in brain tissue, we investigated the expression of IL-1 (alpha and beta) and IL-1 receptor antagonist (IL-1ra) mRNAs in various structures of the central nervous system, as well as in the spleen, following intraperitoneal injection of LPS (100 micrograms/mouse). After RNA extraction and amplification by the reverse transcription-polymerase chain reaction (RT-PCR), the PCR products were separated on an agarose gel, transferred and hybridized with digoxigenin-labeled probes synthetized by nested PCR. Glyceraldehyde phosphate deshydrogenase mRNA was used as an internal control. Under basal conditions the expression of IL-1 alpha, IL-1 beta and IL-1ra mRNAs in the brain was extremely low for the three cytokines; in the spleen these mRNAs were clearly detectable. Following LPS stimulation, mRNAs were strongly increased in all the tested tissues (cortex, hippocampus, hypothalamus, cerebellum, pituitary and spleen). The kinetics of mRNAs expressions in the brain were similar for all the tested regions, with a maximum at 6 h and a decrease up to 24 h after LPS administration. In the spleen the maximum was observed as soon as 1 h following stimulation. In conclusion, peripheral LPS stimulation induces a strong and transient expression of IL-1 alpha and IL-1 beta mRNAs in the brain. IL-1ra mRNA is also stimulated by LPS in various regions of the brain. (ABSTRACT TRUNCATED AT 250 WORDS)
Towards building circular economy

Purpose Extant literature provides insights about consumers’ purchase intentions (PI) and willingness to pay for reconstructed products but does not capture cross-cultural differences. The purpose of this paper is to understand consumers’ PI for reconstructed products in a cross-cultural setting for the development of circular economy. Design/methodology/approach The sample includes consumers from two diverse societies (India and the USA). The authors conduct two sets of analyses in this paper. The first analysis attempts to identify the difference between the Indian and US consumers’ PI for reconstructed products, who are residing permanently in their home countries. In the second analysis, the authors investigate the impact of socio-cultural norms on PI, for reconstructed products, of the Indian (USA) consumers relocated to the USA (India). In-depth interviews are conducted for data collection. Subsequently, data analysis was carried out using thematic analysis approach. Findings The results indicate that US consumers: possess altruistic buying behavior; are eco-centric toward waste disposal; strictly follow the regulations and socio-cultural norms; and have harmony orientation toward nature. In contrast, Indian consumers: possess utilitarian buying behavior; demonstrate anthropocentric attitude toward waste disposal; do not necessarily follow the regulations; and have mastery orientation toward nature. Practical implications At policy level, government in emerging economies should formulate regulations that promote the use of reconstructed products. At firm level, firms should exploit promotional and advertisement efforts to enhance awareness about reconstructed products. Originality/value The study is an early attempt in the literature to capture cross-cultural differences for reconstructed products.