Miracle-dz/UniFAR-FTUs huggingface dataset:
Down-Regulation by Receptor Gene cAMP of Angiotensin II Type Expression in PC 12 Cells 2

The rat angiotensin II type 2 receptor (AT2-R) gene was isolated, and cis-regulatory regions in its 5"flanking area were analyzed. Primer extension and RNase protection analyses revealed a single transcriptional initiation site at the position 24 by downstream of the TATA box. The 5 '-flanking region of AT2-R contained several cis-regulatory elements, such as AP-1, AP-2, C/EBP, NF-1, NF-1L6, NEKB, and glucocorticoidand cAMP-responsive elements (CRE). The treatment of PC12 cells with dibutyryl cAMP caused a marked decrease (90%) in the AT2-R mRNA level, which was blocked by the inhibitor of protein kinase A and did not require new protein synthesis. The protein level was also reduced 84% after a 24-h exposure to cAMP and the binding affinity was unchanged. The half-life of the AT2-R mRNA decreased -66% by cAMP as compared with control (18.4 ± 0.4 h). Deletion and mutation analyses of the 5 '-flanking region (1.2 Kb) revealed that there were one negative (-1,199 to -739) and two positive cis-regulatory regions ( -739 to -436 and -59 to +45), and that the CRE motif located at -426 repressed (-23%) the promoter activity of the rat AT2-R gene. The region between -59 and +45 containing TATA box and AP-2 site accounted for 70% of the promoter activity. These findings indicate that the promoter activity of the rat AT2-R gene is modulated by several cis-regulatory regions and that cAMP markedly downregulates the expression of the AT2-R mainly by inducing AT2-R mRNA destabilization rather than CRE-mediated inhibition of the gene transcription. Thus, humoral factors that transduce cAMP as an intracellular signal may modulate AT2-R-mediated function of Ang II by reducing AT2-R expression. (Hypertens Res 1996; 19: 271-279)
Comparison of Respiratory Pathogen Detection in Upper versus Lower Respiratory Tract Samples Using the BioFire FilmArray Respiratory Panel in the Immunocompromised Host

Background The FilmArray Respiratory Panel (FARP) (BioFire Diagnostics, Inc.) is a multiplex, polymerase chain reaction (PCR) technique that can detect 17 respiratory viruses and 3 bacterial targets in a single reaction. Immunocompromised hosts (ICH) with respiratory illnesses often undergo bronchoscopy with bronchoalveolar lavage (BAL). This prospective study aimed to evaluate the yield and concordance of NP and BAL FARP testing when performed on the same patient concurrently. Methods From February to December 2016, 125 patients (100 ICH and 25 non-ICH) were enrolled. NP swabs and BAL samples were sent for FARP testing. Results The yield of the BAL FARP among ICH and non-ICH was 24% (24/100) and 8% (2/25), respectively. The yield of positive NP swabs in ICH was 27% (27/100) versus 4% (1/25) in non-ICH. The majority of patients (89%; 111/125) had concordant results between NP and BAL specimens. Of the 24 ICH patients who had a positive BAL FARP, the majority (79%) had the same pathogen detected from the NP swab. Conclusion The FARP may be useful in the ICH. Given the high concordance, in patients whom a pathogen is identified on the NP FARP, a FARP performed on BAL will likely yield the same result. However, if the NP FARP is negative, performing the test on a BAL sample may have an incremental yield.
A successful approach to conrol burning mouth syndrome using matricaria recutita and cognitive therapy

The burning mouth syndrome (BMS) has no specific clinical and laboratory signs. Its etiology is yet to be elucidated, but it is considered to be affected by multifactorial, psychological, and local and systemic factors. This condition is considered of great morbidity, and the main complaint of patients maybe associated with xerostomia, thirst, and altered taste. The present study aims to report two cases of BMS and to evaluate the outcome of cognitive therapy (CT) plus phytotherapy in the control of BMS. The patients were female, Caucasian, and aged between 58 and 69 years. The most BMS-affected anatomical parts were the lips and the tongue. In the clinical approach, oral and systemic evaluation, and disease management with CT plus chamomile tea were done. The patients were reassured, and their response to therapy one year after was found to be excellent despite few exacerbations in periods of great emotional stress. Thus, we conclude that psychological treatment is vital in the management of BMS, as CT, along with Matricaria recutita phytotherapy, displayed excellent results in the control of BMS. Key words:Anxiety, chamomile tea, xerostomia, psychosomatic.
Viscous anisotropy of textured olivine aggregates: 2. Micromechanical model

The significant viscous anisotropy that results from crystallographic alignment (texture) of olivine grains in deformed upper mantle rocks strongly influences a large variety of geodynamic processes. Our ability to explore the effects of anisotropic viscosity in simulations of these processes requires a mechanical model that can predict the magnitude of anisotropy and its evolution. Unfortunately, existing models of olivine textural evolution and viscous anisotropy are calibrated for relatively small deformations and simple strain paths, making them less general than desired for many large‐scale geodynamic scenarios. Here we develop a new set of micromechanical models to describe the mechanical behavior and textural evolution of olivine through a large range of strains and complex strain histories. For the mechanical behavior, we explore two extreme scenarios, one in which each grain experiences the same stress tensor (Sachs model) and one in which each grain undergoes a strain rate as close as possible to the macroscopic strain rate (pseudo‐Taylor model). For the textural evolution, we develop a new model in which the director method is used to control the rate of grain rotation and the available slip systems in olivine are used to control the axis of rotation. Only recently has enough laboratory data on the deformation of olivine become available to calibrate these models. We use these new data to conduct inversions for the best parameters to characterize both the mechanical and textural evolution models. These inversions demonstrate that the calibrated pseudo‐Taylor model best reproduces the mechanical observations. Additionally, the pseudo‐Taylor textural evolution model can reasonably reproduce the observed texture strength, shape, and orientation after large and complex deformations. A quantitative comparison between our calibrated models and previously published models reveals that our new models excel in predicting the magnitude of viscous anisotropy and the details of the textural evolution. In addition, we demonstrate that the mechanical and textural evolution models can be coupled and used to reproduce mechanical evolution during large‐strain torsion tests. This set of models therefore provides a new geodynamic tool for incorporating viscous anisotropy into large‐scale numerical simulations.
Internal and External Factors That Support Children's Minority First Language and English.

Purpose Sequential bilingual children in the United States often speak 2 languages that have different social statuses (minority-majority) and separate contexts for learning (home-school). Thus, distinct factors may support the development of each language. This study examined which child internal and external factors were related to vocabulary skills in a minority language versus English. Method Participants included 69 children, aged 5-8 years, who lived in Southern California, spoke Vietnamese as the home language, and received school instruction in English. All participants had at least 1 foreign-born parent, and most mothers reported limited English proficiency. Parents completed a telephone survey, and children completed measures of receptive and expressive vocabulary in each language. Using correlations and stepwise regression, we examined predictors of vocabulary skills in each language that were internal to the child (age, gender, analytical reasoning, phonological memory) or that pertained to the surrounding environment (cumulative exposure, quantity and quality of input/output). Results Vietnamese vocabulary outcomes were related to multiple external factors, of which input and enrichment activities were the best predictors. In contrast, English vocabulary outcomes were related to internal factors, of which age and phonological memory were the best predictors. Parental use of Vietnamese contributed to children's Vietnamese vocabulary outcomes but was not related to children's English vocabulary outcomes. Conclusions Vietnamese exposure does not hinder English development. Children from immigrant families are learning English with or without familial support. Rich and frequent exposure and opportunities for practice are essential for the continued development of a minority first language.
Isopropyl Caffeate: A Caffeic Acid Derivative—Antioxidant Potential and Toxicity

Phenolic compounds, among them isopropyl caffeate, possess antioxidant potential, but not without toxicity and/or adverse effects. The present study aimed to evaluate the antioxidant activity and toxicity of isopropyl caffeate through in silico, in vitro and in vivo testing. The results showed that isopropyl caffeate presents no significant theoretical risk of toxicity, with likely moderate bioactivity: GPCR binding, ion channel modulation, nuclear receptor binding, and enzyme inhibition. Isopropyl caffeate induced hemolysis only at the concentrations of 500 and 1000 μg/ml. We observed types A and O erythrocyte protection from osmotic stress, no oxidation of erythrocytes, and even sequestrator and antioxidant behavior. However, moderate toxicity, according to the classification of GHS, was demonstrated through depressant effects on the central nervous system, though there was no influence on water and food consumption or on weight gain, and it did present possible hepatoprotection. We conclude that the effects induced by isopropyl caffeate are due to its antioxidant activity, capable of preventing production of free radicals and oxidative stress, a promising molecule with pharmacological potential.
Abiotic and Biotic Stress Responses in Solanum tuberosum Group Phureja DM1‐3 516 R44 as Measured through Whole Transcriptome Sequencing

This study conducted an in‐depth analysis of the potato (Solanum tuberosum L.) transcriptome in response to abiotic (salinity, drought, and heat) and biotic (Phytophthora infestans, DL‐β‐amino‐n‐butyric acid, and acibenzolar‐s‐methyl) stresses and plant hormone treatment (abscisic acid, 6‐benzylaminopurine, gibberellic acid, and indole‐3‐acetic acid) using ribonucleic acid sequencing (RNA‐seq) of the doubled monoploid S. tuberosum Group Phureja DM1‐3 516 R44 clone. Extensive changes in gene expression were observed with 37% of the expressed genes being differentially expressed in at least one comparison of stress to control tissue. Stress‐inducible genes known to function in stress tolerance or to be involved in the regulation of gene expression were among the highest differentially expressed. Members of the MYB, APETALA2 (AP2)/ethylene‐responsive element binding factor (ERF), and NAM, ATAF1/2, and CUC2 (NAC) transcription factor families were the most represented regulatory genes. A weighted gene co‐expression network analysis yielded 37 co‐expression modules containing 9198 genes. Fifty percent of the genes within these co‐expression modules were specific to a stress condition indicating condition‐specific responses. Cross‐species comparison between potato and Arabidopsis thaliana (L.) Heynh. uncovered differentially expressed orthologs and defined evolutionary conserved genes. Collectively, the transcriptional profiling of RNA‐seq data presented here provide a valuable reference for potato stress responses to environmental factors that is supported by statistically significant differences in expression changes, highly interconnected genes in co‐expression networks, and evidence of evolutionary conservation.
Neuroendocrine Factors and Head and Neck Squamous Cell Carcinoma: An Affair to Remember

Head and neck squamous cell carcinoma (HNSCC) is one of the most aggressive malignancies. Therefore, the major goal of cancer treatment is inhibition of tumor cell growth and of metastasis development. In order to choose the best management option for HNSCC patients, we need to identify reliable prognostic factors and to develop new molecular techniques in order to obtain a better understanding of therapy resistance. By acting as neurohormones, neurotransmitters, or neuromodulators, the neuroendocrine factors are able to signal the maintenance of physiological homeostasis or progression to malignant disease. Certain neuropeptides possess strong antitumor properties acting as tumor suppressors and immunomodulators, providing additional benefits for future potential therapeutic strategies. In light of the current understanding, cancer starts as a localized disease that can be effectively treated if discovered on proper time. Unfortunately, more than often cancer cells migrate to the surrounding tissues generating distant metastases, thus making the prognosis and survival in this stage much worse. As cellular migration is mandatory for tumor invasion and metastasis development, searching for alternate controllers of these processes, such as the neuroendocrine factors, it is an active tremendous task.
Aspirin Effect on Adverse Pregnancy Outcomes Associated With Stage 1 Hypertension in a High-Risk Cohort

Recently, the American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines revised the recommendations for diagnosis of chronic hypertension. The new classification system includes a diagnosis of stage 1 hypertension in adults with blood pressures 130 to 139/80 to 89 mm Hg. We sought to compare outcomes among women at high risk for preeclampsia with stage 1 hypertension and assessed whether women with stage 1 hypertension had benefit from aspirin treatment compared with high-risk normotensive women. We performed a secondary analysis of the high-risk aspirin trial and included women with prior preeclampsia or diabetes mellitus. Among these women, 827 (81%) were classified as normotensive, whereas 193 (19%) were classified as stage 1 hypertensive. Among women receiving placebo, preeclampsia occurred significantly more often in women with stage 1 hypertension compared with normotensive high-risk women after adjustment for maternal age and body mass index (39.1% versus 15.1%; risk ratio, 2.49; 95% confidence interval, 1.74–3.55). Further, women with stage 1 hypertension had a significant risk reduction related to aspirin prophylaxis (risk ratio, 0.61; 95% confidence interval, 0.39–0.94) that was not seen in normotensive high-risk women (risk ratio, 0.97; 95% confidence interval, 0.70–1.34). Application of the American College of Cardiology/American Heart Association guidelines in a high-risk population demonstrates that in the setting of other risk factors, the presence of stage 1 hypertension is associated with a significantly increased risk of preeclampsia when compared with high-risk normotensive women. These findings emphasize the importance of recognition of stage 1 hypertension as an additive risk factor in women at high risk for preeclampsia and the benefit of aspirin.
Spectral Efficiency Analysis of Cell-Free Massive MIMO Systems With Zero-Forcing Detector

In this paper, we firstly derive two approximations of the achievable uplink rate with the perfect/imperfect channel state information (CSI) in cell-free massive multi-input multi-output (MIMO) systems, and all these approximations are not only in the simple, but also converge into the classical bounds achieved in conventional massive MIMO systems where the base-station (BS) antennas are co-located. It is worth noting that the obtained two approximations with perfect CSI could be regarded as the special cases of the obtained two approximations with imperfect CSI when the pilot sequence power becomes infinite, respectively. Moreover, the theory analysis shows that all obtained approximations with perfect/imperfect CSI have an asymptotic lower bound <inline-formula> <tex-math notation="LaTeX">$\frac {\alpha }{2}\log _{2} L$ </tex-math></inline-formula> thanks to the extra <italic>distance diversity</italic> offered by massively distributed antennas, where <inline-formula> <tex-math notation="LaTeX">$L$ </tex-math></inline-formula> is the number of BS antennas and the path-loss factor <inline-formula> <tex-math notation="LaTeX">$\alpha >2$ </tex-math></inline-formula>, except for the free space environment. Obviously, these results indicate that the cell-free massive MIMO system has huge potential of spectral efficiency than the conventional massive MIMO system with the asymptotically tight bound <inline-formula> <tex-math notation="LaTeX">$\log _{2} L$ </tex-math></inline-formula>.
Spinal Tuberculosis: Rethinking an Old Disease

Despite of the fact that the earliest cases of Spinal Tuberculosis date from 2400 BC, the first modern description was made in 1779 by Sir Percival Pott in the European population. The improvement of public health measures and the introduction of effective anti-tuberculosis drugs have made the infection virtually eradicated in developed countries. However, migration phenomena, the infection by human immunodeficiency virus (HIV) and other causes of immunodeficiency as diabetes and cancer chemotherapy have led to a resurgence of tuberculosis in parts of the world where the disease was sporadic or unknown. It is currently considered a public health problem, both in developed and developing countries. Recently the clinical and radiological features of Spinal Tuberculosis have changed considerably. Atypical presentations are more common nowadays. The improvement of diagnosis and terapeutic management has lead to better clinical outcomes. However, early diagnosis and appropriate treatment remain the mainstay predictors of successful outcomes, preventing the most serious complications of Spinal Tuberculosis: neurological deficits and spinal deformities. The main aim of this review is to discuss the historical aspects of the disease management as well as the most recent challenges. The authors included articles with acceptable design, clearly explained results and justified conclusions according to the data, regardless of their time of publication.
Association between Rheumatoid Arthritis and Respiratory Allergic Diseases in Korean Adults: A Propensity Score Matched Case-Control Study

Rheumatoid arthritis (RA) and allergic diseases are result of a poor functioning immune system, giving dominance to either T-helper 1 (Th1) or T-helper 2 (Th2) diseases, respectively. Studies have stated that there seems to be a relationship present between the immune response subsets. This study was designed to examine the association between RA and respiratory allergic diseases in Korean adults. The study utilized the KNHANES 2013–2015 data and excluded individuals diagnosed with RA before being diagnosed with allergic diseases, using age at clinical diagnosis. Total of 253 RA patients were matched 1 : 1 with non-RA patients by a propensity score, using sex and age as matched variables. Multivariate conditional logistic regression analyses were used to evaluate for association between RA and respiratory allergic diseases in the matched 506 participants. RA was associated with an increased risk of prevalence of respiratory allergic diseases with an OR of 1.51 (95% CI, 1.31–1.75), adjusted for socioeconomic demographic variables. The adjusted OR for prevalence of RA among participants with prevalence of asthma and allergic rhinitis was as follows: 3.12 (95% CI, 2.77–3.51) and 1.39 (95% CI, 1.16–1.67). Participants with prevalence of asthma in particular had an increased risk of developing prevalence of RA. Based on our findings, Th1 and Th2 diseases may indeed coexist, and one pathway may stimulate or contribute towards the onset of the other.
Ganoderma lucidum and Auricularia polytricha Mushrooms Protect against Carbofuran-Induced Toxicity in Rats

The current study aimed to investigate the ameliorative effects of two types of mushrooms, Ganoderma lucidum (GL) and Auricularia polytricha (AP), against carbofuran- (CF) induced toxicity in rats. Male Wistar rats (n = 42) were divided into six equal groups. The rats in the negative control group received oral administration of CF at 1 mg/kg with the normal diet for 28 days. The treatment groups received oral administration of ethanolic extract of GL or AP at 100 mg/kg followed by coadministration of CF at 1 mg/kg with the normal diet for the same experimental period, respectively. In the CF alone treated group, there were significant decreases in the erythrocytic and thrombocytic indices but increases in the concentrations of the total leukocytes, including the agranulocytes. A significant increase in all of the liver function biomarkers except albumin, in lipid profiles except high-density lipoprotein, and in the kidney function markers occurred in the negative control group compared to the rats of the normal control and positive control groups. The coadministration of mushroom extracts significantly ameliorated the toxic effects of the CF. The GL mushroom extract was more efficacious than that of the AP mushroom, possibly due to the presence of high levels of phenolic compounds and other antioxidants in the GL mushroom.
Use of a 3D Floating Sphere Culture System to Maintain the Neural Crest-Related Properties of Human Dental Pulp Stem Cells

Human dental pulp is considered an interesting source of adult stem cells, due to the low-invasive isolation procedures, high content of stem cells and its peculiar embryological origin from neural crest. Based on our previous findings, a dental pulp stem cells sub-population, enriched for the expression of STRO-1, c-Kit, and CD34, showed a higher neural commitment. However, their biological properties were compromised when cells were cultured in adherent standard conditions. The aim of this study was to evaluate the ability of three dimensional floating spheres to preserve embryological and biological properties of this sub-population. In addition, the expression of the inwardly rectifying potassium channel Kir4.1, Fas and FasL was investigated in 3D-sphere derived hDPSCs. Our data showed that 3D sphere-derived hDPSCs maintained their fibroblast-like morphology, preserved stemness markers expression and proliferative capability. The expression of neural crest markers and Kir4.1 was observed in undifferentiated hDPSCs, furthermore this culture system also preserved hDPSCs differentiation potential. The expression of Fas and FasL was observed in undifferentiated hDPSCs derived from sphere culture and, noteworthy, FasL was maintained even after the neurogenic commitment was reached, with a significantly higher expression compared to osteogenic and myogenic commitments. These data demonstrate that 3D sphere culture provides a favorable micro-environment for neural crest-derived hDPSCs to preserve their biological properties.