Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of schedule of reinforcement on cue-elicited reinstatement of cocaine-seeking behavior

Cocaine-associated cues can elicit incentive motivational effects that drive cocaine-seeking behavior and contribute to relapse. The extinction/reinstatement model is commonly used to measure these effects in animals. This study examined the influence of training and testing schedules of reinforcement on cue-elicited reinstatement. Lever presses during training resulted in cues and cocaine (0.75 mg/kg/IV) on either continuous or partial reinforcement schedules [fixed ratio (FR) 1 or 11, variable ratio (VR) 5 or 11]. Animals then underwent extinction training, followed by a test for cue-elicited reinstatement of extinguished cocaine-seeking behavior by response-contingent cue presentations on either a continuous (FR 1) or a partial reinforcement schedule (FR 11). Partial reinforcement during training resulted in higher response rates during cue-elicited reinstatement relative to continuous reinforcement. In contrast, delivery of cues on a continuous reinforcement schedule during testing yielded higher response rates relative to delivery on a partial reinforcement schedule. Finally, the shift from a partial to a continuous reinforcement schedule across training and testing phases did not alter response rates. These findings provide important information for choosing parameters for reinstatement of drug-seeking behavior that would allow the most sensitive method to detect changes in response rate after an experimental manipulation.
Liver‐specific loss of β‐catenin results in delayed hepatocyte proliferation after partial hepatectomy

Recent studies have suggested that β‐catenin is involved in the regulation of hepatocyte proliferation in multiple contexts, including organ development and tumorigenesis. We explored the role of β‐catenin during liver regeneration using T cell factor/lymphoid enhancer factor (TCF/LEF)‐reporter mice (TOPGal mice) and liver‐specific β‐catenin knockout mice. Liver‐specific β‐catenin knockout mice showed a delayed onset of DNA synthesis after hepatectomy, whereas recovery of liver mass was not affected. Among putative β‐catenin target genes examined, the induction of Ccnd1 expression was reduced, whereas the expression of Myc and Egfr was unaffected. Furthermore, cyclin D1 protein levels were not induced, and the expression of cyclins A, E, and proliferating cell nuclear antigen was delayed. Intriguingly, the analysis of TOPGal mice showed that hepatocytes with active TCF/LEF transcription are confined to the pericentral zone and are not increased in number during regeneration, indicating an uncoupling between β‐catenin/TCF signaling activity and hepatocyte proliferation. Conclusion: Our results indicate that β‐catenin is critical for the proper regulation of hepatocyte proliferation during liver regeneration; however, the activity of β‐catenin/TCF signaling does not correlate with hepatocyte proliferation, suggesting that this regulation might be indirect/secondary. (HEPATOLOGY 2007;45:361–368.)
Mutagenic selectivity at the HPRT locus in V-79 cells: comparison of mutations caused by bay-region benzo[a]pyrene 7,8-diol-9,-10-epoxide enantiomers with high and low carcinogenic activity.

Earlier studies from our laboratories characterized the mutation profile of the optically active (+)-7R,8S-dihydroxy-9S,10R-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene [(+)-BPDE--the ultimate carcinogenic metabolite of benzo[a]pyrene] in the coding region of the hypoxanthine (guanine) phosphoribosyltransferase (HPRT) gene of Chinese hamster V-79 cells. In the present study, we evaluated the mutation profile of (-)-7S,8R-dihydroxy-9R, 10S-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene [(-)-BPDE-a weakly carcinogenic or inactive enantiomer] and compared its mutation profile with that of (+)-BPDE. In both diol epoxide enantiomers, the benzylic 7-hydroxy group and epoxide oxygen are trans. The mutation frequency for V-79 cells treated with DMSO vehicle or with a low, non-cytotoxic dose (0.5 microM) or a high cytotoxic dose (2.0 microM) of (-)-BPDE was 1, 25 or 185 8-azaguanine-resistant colonies/10(5) survivors, respectively. Independent 8-azaguanine-resistant clones were isolated, and complementary DNAs were prepared by reverse transcription. The coding region of the HPRT gene was amplified by the polymerase chain reaction and sequenced. Altogether, 92 (-)-BPDE-induced mutant clones were examined. At both doses, base substitutions were the most prevalent mutations observed (present in approximately 7% of the mutant clones), followed by exon deletions (present in approximately 22% of the mutant clones) and frame shift mutations (present in approximately 6% of the mutant clones) in the cDNAs analyzed. At the high cytotoxic dose, 5 out of 36 base substitutions occurred at AT base pairs (14%) and 31 at GC base pairs (86%). At the low, non-cytotoxic dose, 7 out of 34 base substitutions were at AT base pairs (21%) and 27 were at GC base pairs (79%). Although there was a trend towards an increase in the proportion of mutations at AT base pairs when the dose of (-)-BPDE was decreased, this trend was not statistically significant. The data also indicated no dose-dependent differences in the kinds of base substitutions or exon deletions in cDNAs induced by (-)-BPDE. Ninety-one per cent of the (-)-BPDE-induced mutations that occurred at guanine were on the non-transcribed strand of DNA and 9% were on the transcribed strand. In contrast to these results, 50% of the (-)-BPDE-induced mutations that occurred at adenine were on the transcribed strand and 50% on the non-transcribed strand. (ABSTRACT TRUNCATED AT 400 WORDS)
Purification and Molecular Characterization of cGMP-dependent Protein Kinase from Apicomplexan Parasites

The trisubstituted pyrrole 4-[2-(4-fluorophenyl)-5-(1-methylpiperidine-4-yl)-1H-pyrrol-3-yl]pyridine (Compound 1) inhibits the growth of Eimeria spp. bothin vitro and in vivo. The molecular target of Compound 1 was identified as cGMP-dependent protein kinase (PKG) using a tritiated analogue to purify a ∼120-kDa protein from lysates of Eimeria tenella. This represents the first example of a protozoal PKG. Cloning of PKG from several Apicomplexan parasites has identified a parasite signature sequence of nearly 300 amino acids that is not found in mammalian or DrosophilaPKG and which contains an additional, third cGMP-binding site. Nucleotide cofactor regulation of parasite PKG is remarkably different from mammalian enzymes. The activity of both native and recombinantE. tenella PKG is stimulated 1000-fold by cGMP, with significant cooperativity. Two isoforms of the parasite enzyme are expressed from a single copy gene. NH2-terminal sequence of the soluble isoform of PKG is consistent with alternative translation initiation within the open reading frame of the enzyme. A larger, membrane-associated isoform corresponds to the deduced full-length protein sequence. Compound 1 is a potent inhibitor of both soluble and membrane-associated isoforms of native PKG, as well as recombinant enzyme, with an IC50 of <1 nm.
Demand analysis in Angola seaports

ABSTRACT This paper presents a demand analysis of Angola seaports from 1996 to 2013 using the Berry, Levinsohn, and Pakes (BLP) demand model. The BLP is a random coefficient Logit demand model that takes into account the endogeneity of the price in the demand equation. The model reveals that seaports on Angola is explained by the average price, the price of maritime transport services, the price of substitute imports by airports, and by the income in the port region. The price is endogenous in demand equation and the endogeneity is taken into account in demand estimation. The price of air transportation is negative, and therefore it is a complementary good. The price of container handling is positive, and therefore it is a substitution good. Policy implication is also derived.
Some observations on the possible nutritional significance of vitamin B12-and folate-binding proteins in milk. Absorption of [58Co] cyanocobalamin by suckling piglets

1. A study was made of the absorption of [58Co]cyanocobalamin in suckling piglets. Cyanocobalamin given at birth and at 7 d of age was efficiently absorbed from the intestine and retained within the body, mostly in the liver. A 10 μg test dose was absorbed no less efficiently than 0.5 μg, despite the virtual absence of intrinsic factor in the gut. In piglets given a 10 μg test dose at different ages between 0.5 and 56 d, there was a marked decrease in the efficiency of retention between about 7 and 21 d of age. 2. Vitamin B12-binding capacity in the gastric mucosa increased with age, from 40 ng at birth to about 2000 ng at 14 d and 7000 ng at 35 d. This binder-protein was largely endogenous, whereas much of the unsaturated binder-protein in intestinal mucosa was apparently derived from milk. 3. The chyme in the stomach and small intestine contained unsaturated binder-protein, partly endogenous and partly deriving from milk, which prevented uptake of added [G-3H]-cyanocobalamin into the ‘solids’ phase of the intestinal contents. The intestinal chyme contained large numbers (log10 7.0–9.1/ml) of bacteria, some of which were isolated and shown to take up cyanocobalamin or folic acid or both. 4. The findings are discussed in relation to the concept that for some days or weeks after the cessation of transport of intact protein across the neonatal gut epithelium (‘closure’), protein-bound vitamins may continue to be taken up into the epithelial cells and there released for transport into the circulation. It is concluded that unsaturated vitamin-binders may strongly influence the ecology of the intestinal microflora.
Capital formation in Latin America: one and a half century of macroeconomic dynamics

Macroeconomic studies indicate that physical capital formation has played a pivotal role in long-term economic growth. These studies have been hampered, however, by a data constraint: in order to pinpoint exactly what the role of capital formation has been, a larger empirical database –larger in terms of both the time span and the geographical area covered– is needed. This study addresses that problem by providing new and very extensive series on capital formation in Latin America. It also describes the different series used to identify long, medium and short-term movements. One of the outstanding features of these investment trends were their marked instability up to 1950. Another salient aspect has been the more robust growth in investment seen in the second half of the nineteenth century, which actually outdistanced the growth spurt that occurred during the “golden age” of 1950-1980.
Genetic variation in Rhodomyrtus tomentosa (Kemunting) populations from Malaysia as revealed by inter-simple sequence repeat markers.

Kemunting (Rhodomyrtus tomentosa) from the Myrtaceae family, is native to Malaysia. It is widely used in traditional medicine to treat various illnesses and possesses significant antibacterial properties. In addition, it has great potential as ornamental in landscape design. Genetic variability studies are important for the rational management and conservation of genetic material. In the present study, inter-simple sequence repeat markers were used to assess the genetic diversity of 18 R. tomentosa populations collected from ten states of Peninsular Malaysia. The 11 primers selected generated 173 bands that ranged in size from 1.6 kb to 130 bp, which corresponded to an average of 15.73 bands per primer. Of these bands, 97.69% (169 in total) were polymorphic. High genetic diversity was documented at the species level (H(T) = 0.2705; I = 0.3973; PPB = 97.69%) but there was a low diversity at population level (H(S) = 0.0073; I = 0 . 1085; PPB = 20.14%). The high level of genetic differentiation revealed by G(ST) (73%) and analysis of molecular variance (63%), together with the limited gene flow among population (N(m) = 0.1851), suggests that the populations examined are isolated. Results from an unweighted pair group method with arithmetic mean dendrogram and principal coordinate analysis clearly grouped the populations into two geographic groups. This clear grouping can also be demonstrated by the significant Mantel test (r = 0.581, P = 0.001). We recommend that all the R. tomentosa populations be preserved in conservation program.
MILLIMETER OBSERVATIONS OF A SAMPLE OF HIGH-REDSHIFT OBSCURED QUASARS

We present observations at 1.2 mm with Max-Planck Millimetre Bolometer Array (MAMBO-II) of a sample of z ≳ 2 radio-intermediate obscured quasars, as well as CO observations of two sources with the Plateau de Bure Interferometer. The typical rms noise achieved by the MAMBO observations is 0.55 mJy beam−1 and five out of 21 sources (24%) are detected at a significance of ⩾3σ. Stacking all sources leads to a statistical detection of 〈S1.2 mm〉 = 0.96 ± 0.11 mJy and stacking only the non-detections also yields a statistical detection, with 〈S1.2 mm〉 = 0.51 ± 0.13 mJy. At the typical redshift of the sample, z = 2, 1 mJy corresponds to a far-infrared luminosity LFIR∼4 × 1012 L☉. If the far-infrared luminosity is powered entirely by star formation, and not by active galactic nucleus heated dust, then the characteristic inferred star formation rate is ∼700 M☉ yr−1. This far-infrared luminosity implies a dust mass of Md∼3 × 108 M☉, which is expected to be distributed on ∼kpc scales. We estimate that such large dust masses on kpc scales can plausibly cause the obscuration of the quasars. Combining our observations at 1.2 mm with mid- and far-infrared data, and additional observations for two objects at 350 μm using SHARC-II, we present dust spectral energy distributions (SEDs) for our sample and derive a mean SED for our sample. This mean SED is not well fitted by clumpy torus models, unless additional extinction and far-infrared re-emission due to cool dust are included. This additional extinction can be consistently achieved by the mass of cool dust responsible for the far-infrared emission, provided the bulk of the dust is within a radius ∼2–3 kpc. Comparison of our sample to other samples of z ∼ 2 quasars suggests that obscured quasars have, on average, higher far-infrared luminosities than unobscured quasars. There is a hint that the host galaxies of obscured quasars must have higher cool-dust masses and are therefore often found at an earlier evolutionary phase than those of unobscured quasars. For one source at z = 2.767, we detect the CO(3–2) transition, with SCOΔν = 630 ± 50 mJy km s−1, corresponding to LCO(3-2) = 3.2 ×107 L☉, or a brightness-temperature luminosity of L′CO(3-2) = 2.4 × 1010 K km s−1 pc2. For another source at z = 4.17, the lack of detection of the CO(4–3) line suggests the line to have a brightness-temperature luminosity L′CO(4-3) < 1 × 1010 K km s−1 pc2. Under the assumption that in these objects the high-J transitions are thermalized, we can estimate the molecular gas contents to be M☉ and <8 × 109 M☉, respectively. The estimated gas depletion timescales are τg = 4 Myr and <16 Myr, and low gas-to-dust mass ratios of Mg/Md = 19 and <20 are inferred. These values are at the low end but consistent with those of other high-redshift galaxies.
Intended Use of Proceeds, Underwriter Quality and the Long‐Run Performance of SEOs in the UK

We document that prospectus disclosure of (i) the motives for a seasoned equity offering, and (ii) the choice of underwriter explain the long-run performance of equity issuers in the UK. Firms citing investment needs show no abnormal performance after the offering and have higher investment rates post-issue compared to the period before the offering. Issuers that state general corporate purposes and recapitalisation motives underperform, have similar investment rates pre- and post-issue, and their leverage tends to increase after the offering. Further, consistent with the certifying role of underwriters, equity issues underwritten by high-quality brokers show no evidence of post-issue abnormal returns, but offerings taken public by low-quality underwriters exhibit negative abnormal performance. Together, our results document the significant role that prospectus information on the intended use of offering proceeds and on the underwriter play in predicting issuers post-offering performance in the UK.
THE STRUCTURE OF LATTICES OF EXISTENTIAL FORMULAS FOR FRAGMENT OF JONSSON SETS

This article is related to one of the branches of Model Theory, more precisely to the study of Jonsson sets. This part of Model Theory is concerned with the study of incomplete inductive theories, namely with Jonsson theories and some of their generalizations. It examines the model-theoretical properties of Jonsson theories. In particular the lattice of special formulas is considered. In the study of complete theories one of the main methods is to use the properties of a topological space Sn(T ). In the case of Jonsson theory, we can consider the lattice En(Fr(X)) of existential formulas, which is a sublattice of the Boolean algebra Fn(Fr(X)). The main aim of this article is to develop the basic concepts and methods of that part of the Model Theory which will provide an opportunity to fruitful studies of Jonsson theories and some of its generalizations. Our technique is a standard method of the study of incomplete theories. It involves the translation of the elementary properties of the center of a Jonsson theory into the theory
Acne scarring treatment using skin needling

Background. Acne is a common condition seen in up to 80% of people between 11 and 30 years of age and in up to 5% of older adults. In some patients, it can result in permanent scars that are surprisingly difficult to treat. A relatively new treatment, termed skin needling (needle dermabrasion), seems to be appropriate for the treatment of rolling scars in acne.
Wnt5a is required for proper epithelial-mesenchymal interactions in the uterus

Epithelial-mesenchymal interactions play a crucial role in the correct patterning of the mammalian female reproductive tract (FRT). Three members of the Wnt family of growth factors are expressed at high levels in the developing FRT in the mouse embryo. The expression of Wnt genes is maintained in the adult FRT, although levels fluctuate during estrous. Wnt4 is required for Müllerian duct initiation, whereas Wnt7a is required for subsequent differentiation. In this study, we show that Wnt5a is required for posterior growth of the FRT. We further demonstrate that the mutant FRT has the potential to form the posterior compartments of the FRT using grafting techniques. Postnatally, Wnt5a plays a crucial role in the generation of uterine glands and is required for cellular and molecular responses to exogenous estrogens. Finally, we show that Wnt5a participates in a regulatory loop with other FRT patterning genes including Wnt7a, Hoxa10 and Hoxa11. Data presented provide a mechanistic basis for how uterine stroma mediates both developmental and estrogen-mediated changes in the epithelium and demonstrates that Wnt5a is a key component in this process. The similarities of the Wnt5a and Wnt7a mutant FRT phenotypes to those described for the Hoxa11 and Hoxa13 mutant FRT phenotypes reveal a mechanism whereby Wnt and Hox genes cooperate to pattern the FRT along the anteroposterior axis.
Audiometric Outcome Comparison between Titanium Prosthesis and Molded Autologous Material

Objective To evaluate and compare the audiometric outcomes of ossicular reconstruction with titanium prosthesis and autologous material. Study Design Retrospective cohort study. Setting Tertiary referral center. Subjects and Methods Audiometric results of consecutive patients who performed ossiculoplasty with titanium prosthesis (n = 43) or autologous material (n = 48), from October 2008 to December 2011, were reviewed retrospectively and compared. The association between air-bone gain and age, ossiculoplasty material (autologous or titanium), preoperative diagnosis (chronic otitis media without cholesteatoma, cholesteatoma, or conductive hearing loss with intact tympanic membrane), and type of surgery (tympanoplasty, canal wall-down mastoidectomy, or canal wall-up mastoidectomy) was explored using regression analysis. Results Preoperative audiometric evaluation did not show differences between titanium and autologous groups in air-bone gap and pure-tone average. A postoperative decrease of 11.0 dB in air-bone gap and 12.4 dB in pure-tone average was observed in titanium ossiculoplasty compared with a reduction of 4.0 dB in air-bone gap and 5.1 dB in pure-tone average when autologous reconstruction was used. Mann-Whitney test revealed superior results in the titanium group compared with autologous reconstruction patients in air-bone gap (P = .02) and pure-tone average (P = .02). However, no statistically significant associations were observed after multivariate linear regression analysis of air-bone gap gain when adjusted for age, ossiculoplasty material, type of surgery, and preoperative diagnosis. One titanium prosthesis extrusion occurred during follow-up (2%). Conclusion Audiometric results achieved by titanium prosthesis are promising, but significant differences compared with autologous ossiculoplasty were not present.
Phytoestrogenic activity of blackcurrant (Ribes nigrum) anthocyanins is mediated through estrogen receptor alpha.

SCOPE Blackcurrants (Ribes nigrum L., Grossulariaceae) contain high amounts of anthocyanin polyphenols, which have antioxidant and anti-carcinogenic health benefits. This study analyzed the potential phytoestrogenic effects of blackcurrant extract (BCE) in breast cancer (MCF-7) and human endometrial cancer (Ishikawa) cell lines that over-express estrogen receptor alpha (ERα), as well as in immature female rats. METHODS AND RESULTS Microarray analysis and Ingenuity® Pathway Analysis showed that BCE activated the ERα pathway, whereas quantitative-PCR confirmed that BCE and four types of anthocyanins up-regulated genes downstream of ERα. BCE (0.1-1.0 μg/mL) and anthocyanins (0.1-10 μM) induced MCF-7 cell proliferation; however, this effect was blocked by ER antagonist fulvestrant. Flow cytometry showed that anthocyanins reduced and increased the number of MCF-7 cells in the G0/G1 and G2/M phases, respectively. Anthocyanins stimulated ERα transcriptional activity in human ERα reporter assays and induced alkaline phosphatase activity in Ishikawa cells. Competition assays and in silico analysis indicated that anthocyanins bind to ERα. Finally, BCE focally induced stratification of columnar epithelial cells in the rat uterus and increased cytoplasmic mucin levels in these cells. CONCLUSION These results suggest that blackcurrant anthocyanins act as phytoestrogens in vitro and in vivo.