Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii

Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content.
A case report of an aggressive rhabdomyosarcoma associated with non-puerperal uterine inversion

We report a case of Non-puerperal Uterine Inversion secondary to an aggressive embryonal rhabdomyosarcoma. Nonpuerperal Uterine Inversion is a rare clinical entity and many gynaecologists may not come across this in their practice (Rattray et al. 2000). Non-puerperal uterine inversions can be caused by benign (70%), most commonly submucous leiomyomas with a fundal attachment, or malignant tumours (20%) (Mwinyoglee et al. 1997). Malignant mixed mesodermal tumours (MMMTs) or carcinosarcomas are an uncommon cause of uterine inversion (occur in 5% of all uterine tumours) (Kanthan and Senger 2011; Banik et al. 2012). Rhabdomyosarcoma (RMS), is an aggressive and highly malignant tumour that may arise anywhere in the body, predominant locations include: the head and neck, genitourinary areas and the retroperitoneum. According to the Intergroup Rhabdomyosarcoma study (IRSG) of 1972–1984, approximately 3% of RMS originates in the uterus and the vagina. Vaginal RMS is most commonly seen in infants and young children with an average age of 23.5months, while uterine RMS is more common in adolescents, the mean age being 14 years (Maurer et al. 1988). To-date, 32 cases of uterine inversion due to sarcoma have been reported with only five cases of rhabdomyosarcoma (RMS) including our case since 1887 (Occhionero et al. 2012).
DNA sequence models of genome-wide Drosophila melanogaster Polycomb binding sites improve generalization to independent Polycomb Response Elements

Abstract Polycomb Response Elements (PREs) are cis-regulatory DNA elements that maintain gene transcription states through DNA replication and mitosis. PREs have little sequence similarity, but are enriched in a number of sequence motifs. Previous methods for modelling Drosophila melanogaster PRE sequences (PREdictor and EpiPredictor) have used a set of 7 motifs and a training set of 12 PREs and 16-23 non-PREs. Advances in experimental methods for mapping chromatin binding factors and modifications has led to the publication of several genome-wide sets of Polycomb targets. In addition to the seven motifs previously used, PREs are enriched in the GTGT motif, recently associated with the sequence-specific DNA binding protein Combgap. We investigated whether models trained on genome-wide Polycomb sites generalize to independent PREs when trained with control sequences generated by naive PRE models and including the GTGT motif. We also developed a new PRE predictor: SVM-MOCCA. Training PRE predictors with genome-wide experimental data improves generalization to independent data, and SVM-MOCCA predicts the majority of PREs in three independent experimental sets. We present 2908 candidate PREs enriched in sequence and chromatin signatures. 2412 of these are also enriched in H3K4me1, a mark of Trithorax activated chromatin, suggesting that PREs/TREs have a common sequence code.
Food Security Status and Hair Cortisol among Low-income Mother-Child Dyads

This cross-sectional study among low-income mother-child dyads examined the relationship between food security status and hair cortisol concentration (HCC), and how mother and child food security status was interactively related to mothers’ perceived distress and coping. Data from 32 dyads were analyzed. Approximately 46.9% had household food insecurity, 37.5% children had food insecurity, and 34.4% mothers had food insecurity. Children with food insecurity had significantly higher HCC than those with food security. A significant interaction effect occurred between child food security status and race on children’s HCC, with White children’s HCC being more likely to be influenced by food security status. Mother food security and child food security status together explained about 6% of the variance in mothers’ HCC, 27% in perceived distress, and 18% in perceived coping. These findings indicate that assisting low-income families with food insecurity to get enough food can benefit both children’s and mothers’ mental health.
Prevalence of depression and anxiety and their association with cardiovascular risk factors in Northeast Brasil primary care patients.

BACKGROUND Depression and anxiety disorders (DAD) are the most prevalent mental health conditions worldwide. Among the adult population served in basic care, it is estimated that depression affects about 14.3% of these individuals worldwide, and between 21.4% and 31% in Brasil. Anxiety affects up to 33.7% of the population during their lifetimes. OBJECTIVES estimate the prevalence proportions of DAD among patients in a municipality in Northeast Brasil and study the association between DAD and cardiovascular risk factors. METHODS a cross-sectional study with the medical records of patients from primary care centers in Jaboatão dos Guararapes, Pernambuco. Patients aged ≥ 18 years and regularly followed-up were included. Exclusion criteria: a history of traumatic brain injury, alcohol or drug abuse, previous stroke, medical conditions or medications that mimic DAD symptoms. Subjects were divided into two groups depending on the presence or absence of DAD, and cardiovascular risk factors were compared between groups. RESULTS A total of 1030 subjects were initially included, of whom 215 (20%) were excluded. No-DAD subjects had more history of myocardial infarction and alcoholism. The prevalence of depression was 10.3%, anxiety disorder was 27.1%, and mixed DAD represented 4.5%. There was a significant association between DAD and hypertension (OR = 2.11; 95%CI: 1.16 -3.84; p=0.01), obesity (OR = 4.47; 95%CI: 1.74 -11.46; p=0.002), and hyperlipidemia (OR = 3.88; 95%CI: 1.81-8.3; p<0.001). CONCLUSION DAD were associated with an increased risk for arterial hypertension, obesity, and hyperlipidemia.
Area Effects of Color-identification on A CRT Display in the Twilight Condition

An experiment was conducted to investigate the area effects on the color-identification performance in the twilight conditions. The independent variable was the area of color targets whose diameters range from 1.5mm to 14mm. Correct rate was analysed. The results showed that the area effect of color targets was significant. The color-identification performance got better when the diameters of color targets changed from 1.5mm to 14mm.
The Arsenic Binding Aptamer Cannot Bind Arsenic: Critical Evaluation of Aptamer Selection and Binding.

An arsenic binding aptamer named Ars-3 was reported in 2009, and it has been used for detection of As(III) in more than two dozen papers. In this work, we performed extensive binding assays using isothermal titration calorimetry, DNA staining dyes, and gold nanoparticles, respectively. By carefully comparing Ars-3 and a few random control DNA sequences, no specific binding of As(III) was observed in each case. Therefore, we conclude that Ars-3 cannot bind As(III). Possible reasons for some of the previously reported binding and detection were speculated to be related to the adsorption of As(III) onto gold surfaces, which were used in many related sensor designs, and As(III)/Au interactions were not considered before. The selection data in the original paper were then analyzed from sequence alignment, secondary structure prediction, and dissociation constant measurement. These steps need rigorous testing before confirming specific binding of newly selected aptamers. This study calls for attention to the gap between aptamer selection and biosensor design, and the gap needs to be carefully filled by careful binding assays to further the growth of the aptamer field.
Genetic polymorphisms and transcription profiles associated with intracranial aneurysm: a key role for NOTCH3

Intracranial aneurysm (IA) incidence is about 1~2%. However, the specific mechanisms of IA onset and development need further study. Our objective was to discover novel IA-related genes to determine possible etiologies further. We performed next-generation sequencing on nineteen Chinese patients with familial IA and one patient with sporadic IA. We obtained mRNA expression data of 129 samples from Gene Expression Omnibus (GEO) and made statistical computing to discover differentially expressed genes (DEGs). The screened IA-related gene NOTCH3 was determined by bioinformatic data mining. We verified the IA-related indicators of NOTCH3. Association was found between IA and the NOTCH3 SNPs rs779314594, rs200504060 and rs2285981. Levels of NOTCH3 mRNA were lower in IA tissue than in control tissue, but higher in peripheral blood neutrophils from IA patients than in neutrophils from controls. Levels of NOTCH3 protein were lower in IA tissue than in cerebral artery tissue. NOTCH3 also decreased the expression of angiogenesis factors in human umbilical vein endothelial cells. Variation in NOTCH3 and alteration of its expression in cerebral artery or neutrophils may contribute to IA. Our findings also describe a bioinformatic-experimental approach that may prove useful for probing the pathophysiology of other complex diseases.
Biomarker Profiling of Microbial Mats in the Geothermal Band of Cerro Caliente, Deception Island (Antarctica): Life at the Edge of Heat and Cold

Abstract Substrate–atmosphere interfaces in Antarctic geothermal environments are hot–cold regions that constitute thin habitable niches for microorganisms with possible counterparts in ancient Mars. Cerro Caliente hill in Deception Island (active volcano in the South Shetland Islands) is affected by ascending hydrothermal fluids that form a band of warm substrates buffered by low air temperatures. We investigated the influence of temperature on the community structure and metabolism of three microbial mats collected along the geothermal band of Cerro Caliente registering 88°C, 8°C, and 2°C at the time of collection. High-throughput sequencing of small subunit ribosomal ribonucleic acid (SSU rRNA) genes and Life Detector Chip (LDChip) microarray immunoassays revealed different bacterial, archaeal, and eukaryotic composition in the three mats. The mat at 88°C showed the less diverse microbial community and a higher proportion of thermophiles (e.g., Thermales). In contrast, microbial communities in the mats at 2°C and 8°C showed relatively higher diversity and higher proportion of psychrophiles (e.g., Flavobacteriales). Despite this overall association, similar microbial structures at the phylum level (particularly the presence of Cyanobacteria) and certain hot- and cold-tolerant microorganisms were identified in the three mats. Daily thermal oscillations recorded in the substrate over the year (4.5–76°C) may explain the coexistence of microbial fingerprints with different thermal tolerances. Stable isotope composition also revealed metabolic differences among the microbial mats. Carbon isotopic ratios suggested the Calvin–Benson–Bassham cycle as the major pathway for carbon dioxide fixation in the mats at 2°C and 8°C, and the reductive tricarboxylic acid cycle and/or the 3-hydroxypropionate bicycle for the mat at 88°C, indicating different metabolisms as a function of the prevailing temperature of each mat. The comprehensive biomarker profile on the three microbial mats from Cerro Caliente contributes to unravel the diversity, composition, and metabolism in geothermal polar sites and highlights the relevance of geothermal-cold environments to create habitable niches with interest in other planetary environments.
Survey on the research of focused crawling technique

The survey of focused crawling starts with the motivation for this new research and an introduction on basic concepts of focused crawling.The key issues in focused crawling are reviewed,such as webpage analyzing algorithms and the searching strategy on the Web.How to crawl relevant data and information according to different requirements is discussed in detail and three representative architectures of focused crawler systems are analyzed.Some future works for focused crawling research are indicated,including crawling for data analysis and data mining,topic description,finding relevant Web pages,Web data cleaning,and the extension of search space.
Spectral methods for multimodal data analysis

Spectral methods have proven themselves as an important and versatile tool in a wide range of problems in the fields of computer graphics, machine learning, pattern recognition, and computer vision, where many important problems boil down to constructing a Laplacian operator and finding a few of its eigenvalues and eigenfunctions. Classical examples include the computation of diffusion distances on manifolds in computer graphics, Laplacian eigenmaps, and spectral clustering in machine learning. In many cases, one has to deal with multiple data spaces simultaneously. For example, clustering multimedia data in machine learning applications involves various modalities or “views” (e.g., text and images), and finding correspondence between shapes in computer graphics problems is an operation performed between two or more modalities. In this thesis, we develop a generalization of spectral methods to deal with multiple data spaces and apply them to problems from the domains of computer graphics, machine learning, and image processing. Our main construction is based on simultaneous diagonalization of Laplacian operators. We present an efficient numerical technique for computing joint approximate eigenvectors of two or more Laplacians in challenging noisy scenarios, which also appears to be the first general non-smooth manifold optimization method. Finally, we use the relation between joint approximate diagonalizability and approximate commutativity of operators to define a structural similarity measure for images. We use this measure to perform structure-preserving color manipulations of a given image. v
Composing across modes: a comparative analysis of adolescents’ multimodal composing processes

ABSTRACT Although the shift from page to screen has dramatically redefined conceptions of writing, very little is known about how youth compose with multiple modes in digital environments. Integrating multimodality and multiliteracies theoretical frameworks, this comparative case study examined how urban twelfth-grade students collaboratively composed across three multimodal projects when responding to and analyzing literature. Data sources included screen capture and video observations, student design interviews, written reflections, and multimodal products. Findings revealed that multimodal composing was a complex, dynamic, and varied process mediated by the interaction of multiple factors. Students exhibited modal preferences when working with open and flexible digital tools – spending a majority of time working with that particular mode and relying on it to carry the communicative weight of their compositions. The development of multimodal composing timescapes for this study provided new insights into students’ rapid and frequent cross-modal traversals as they worked on their digital projects.
THE E-LEARNING COMPONENT OF A BLENDED LEARNING COURSE

Using new technologies in the academic field has be come more and more visible in Poland in the recent years. In the past, digital learning res ources were used as supplementary materials helping to support face-to-face instruction. Nowada ys, we have the opportunity not only to apply ‘traditional’ methods but also to use more sophisticated approaches such as e-learning and blended learning (BL) to provide more effective and flexible ways of delivering knowledge to students. E-learning can become a very effective part of ‘blended learning’ where a part of the course content is delivered onl ine. This article provides a brief overview of the basic issues that need to be taken into conside ration while designing a blended learning course. More precisely, it focuses on the e-learnin g aspect of blended learning. It also aims at providing the reader with adopted solutions and enc ou tered problems in the process of BL course design.
Development of biosurfactant-based graphene quantum dot conjugate as a novel and fluorescent theranostic tool for cancer

Background Biosurfactants are amphipathic molecules of microbial origin that reduce surface and interfacial tension at gas–liquid–solid interfaces. Earlier, the biosurfactant was isolated and characterized in our laboratory from Candida parapsilosis. The property of the biosurfactant is further explored in this study by using quantum dots (QDs) as nanocarrier. Materials and methods Graphene quantum dots (GQDs) were synthesized by bottom-up approach through pyrolysis of citric acid. GQDs were conjugated with both biosurfactant and folic acid (FA) using carbodiimide chemistry. The prepared GQD bioconjugate was studied for diagnostic and therapeutic effects against cancer cells. Results and discussion Photoluminescence quantum yield (QY) of plain GQDs was measured as 12.8%. QY for biosurfactant conjugated GQDs and FA-biosurfactant conjugated GQDs was measured as 10.4% and 9.02%, respectively, and it was sufficient for targeting cancer cells. MTT assay showed that more than 90% of cells remained viable at concentration of 1 mg/mL, hence GQDs seemed to be non-toxic to cells. Biosurfactant conjugated GQDs caused 50% reduction in cellular viability within 24 hours. FA conjugation further increased the specificity of bioconjugated GQDs toward tumor cells, which is clearly evident from the drug internalization studies using confocal laser scanning microscopy. A higher amount of drug uptake was observed when bioconjugated GQDs were decorated with FA. Conclusion The ability of GQD bioconjugate could be used as a theranostic tool for cancer. It is foreseen that in near future cancer can be detected and/or treated at an early stage by utilizing biosurfactant conjugated GQDs. Therefore, the proposed study would provide a stepping stone to improve the life of cancer patients.
Handbook Of Acoustical Measurements And Noise Control

handbook of acoustical measurements and noise control is available in our digital library an online access to it is set as public so you can download it instantly. Our digital library saves in multiple countries, allowing you to get the most less latency time to download any of our books like this one. Merely said, the handbook of acoustical measurements and noise control is universally compatible with any devices to read.
Assessing Preference of Informal Environmental Education Sources for Greek Primary School Students

This study aims to examine the attitudes of primary school students regarding their interests and habits in relation to the media they use to receive information about them. Using the responses of 412 students from the 5th and 6th grade of primary school in the region of Evros, it is concluded that students’ TV preferences, as well as the means of information they use, differ depending on their interests. The school, TV and the Internet constitute important sources for them, while, concerning TV programmes, comedy series are dominant.
Perception of Teacher Candidates with Regard to Use of Theatre and Drama Applications in Education

Abstract The goal of this study is to identify the perception of mother tongue teachers with regard to the use of theatre and drama in education. In light of this goal, 35 teacher candidates trained in Kutahya Dumlupinar University in the academic year of 2012-2013 were selected as samples. Phenomenology pattern was used in the study as one of the qualitative research patterns, where a survey of 20 open ended questions was used as data collection technique, and the data was analyzed with content analysis. According to the findings of the research, use of theatre or drama in education is shown as the most important reasons for building self-esteem in students and a permanent education for the same. Apart from these, it is also declared that they provide cooperation between students, develop lingual skills and especially speaking skills, they teach how to establish empathy, increase courage, socialize the individual, and strengthen communication. It is also stated as an entertaining and powerful visual art on its own. For these reasons, Theatre and Drama Applications or courses with similar applications should be frequently used in all fields of education.
Raza , Política y Seguridad Pública : Un Estudio de las Patrullas de Autopistas y la Fuerzas Policiales Estatales de los Estados Unidos , 1981-2015

This study assesses the social, political, economic, and traffic-/travel-related predictors of sworn highway patrol and state police strength in the United States between 1981 and 2015. Fixed-effects estimates based on analyses of 1,635 stateyears indicate that theoretical accounts centered on racial threat theory, partisan politics, and gendered politics in part explain variation in this outcome. Findings suggest that changes in population density, the tax base, the percentage of the population without a high school degree, violent crime rates, and spending on social welfare at the state level, as well as shifts in local law enforcement strength, also influence state police and patrol organization strength over this period. Surprisingly, fluctuations in the number of state traffic fatalities per million vehicle miles traveled and the number of driver’s licenses per 100,000 state population—two seemingly important traffic-/travel-related factors—have no impact on the rate of state police and patrol officers per 100,000 population.
Age and the effects of news media attention and social media use on political interest and participation: Do social media function as leveller?

This article investigates how media use differs across age groups- and whether this matters for people’s inclination to participate politically. More specifically, the study investigates the impact of social media use for political purposes and of attention to political news in traditional media, on political interest and offline political participation. The findings, based on a four-wave panel study conducted during the 2010 Swedish national election campaign, show (1) clear differences in media use between age groups and (2) that both political social media use and attention to political news in traditional media increase political engagement over time. Thus, this study suggests that frequent social media use among young citizens can function as a leveller in terms of motivating political participation.