Miracle-dz/UniFAR-FTUs huggingface dataset:
Proton Irradiation Augments the Suppression of Tumor Progression Observed with Advanced Age

Proton radiation is touted for improved tumor targeting, over standard gamma radiation, due to the physical advantages of ion beams for radiotherapy. Recent studies from our laboratory demonstrate that in addition to these targeting advantages, proton irradiation can inhibit angiogenic and immune factors critical to “hallmark” processes that impact cancer progression, thereby modulating tumor development. Outside the therapeutic utilization of protons, high-energy protons constitute a principal component of galactic cosmic rays and thus are a consideration in carcinogenesis risk for space flight. Given that proton irradiation modulates fundamental biological processes known to decrease with aging (e.g. angiogenesis and immunogenicity), we investigated how proton irradiation impacts tumor advancement as a function of host age, a question with both therapeutic and carcinogenesis implications. Tumor lag time and growth dynamics were tracked, after injection of murine Lewis lung carcinoma (LLC) cells into syngeneic adolescent (68 day) vs. old (736 day) C57BL/6 mice with or without coincident irradiation. Tumor growth was suppressed in old compared to adolescent mice. These differences were further modulated by proton irradiation (1 GeV), with increased inhibition and a significant radiation-altered molecular fingerprint evident in tumors grown in old mice. Through global transcriptome analysis, TGFβ1 and TGFβ2 were determined to be key players that contributed to the tumor dynamics observed. These findings suggest that old hosts exhibit a reduced capacity to support tumor advancement, which can be further reduced by proton irradiation.
Do pre-drinkers consume more alcohol than non-pre-drinkers on an event-specific night out? A cross-national panel mobile survey of young people's drinking in England and Denmark.

INTRODUCTION AND AIMS Young people drinking heavily before going out to bars and clubs is associated with alcohol-related harm and therefore of great public concern. This study examines whether pre-drinkers consume more alcohol than non-pre-drinkers on an event-specific night out in England and Denmark--two European countries known for their excessive youth drinking. DESIGN AND METHODS An event-specific survey of 1298 young people conducted in 50 bars, pubs and nightclubs in England and Denmark and follow-up interviews conducted via mobile surveys (n = 580). The questionnaire measured demographics, socioeconomic status, frequency of intoxication and alcohol unit intake before and during the young people's night out. RESULTS A mixed linear model performed on the panel mobile survey shows that pre-drinkers in England and Denmark consume 9.185 (P < 0.001) and 7.554 (P < 0.001) units, respectively, more than the non-pre-drinkers. However, in both countries pre-drinkers consume 3.430 (P < 0.05) and 3.141 (P < 0.001) units less alcohol on-premises than the non-pre-drinkers. DISCUSSION AND CONCLUSION Pre-drinking is a widespread phenomenon in England and Denmark, with more than half of young people pre-drinking on an event-specific night out. Pre-drinking contributes significantly to high-intensity drinking, as it does not preclude further drinking in bars, clubs and pubs. Thus, pre-drinking is a major target for public measures seeking to reduce young people's intoxication-related drinking and alcohol-related harm.
The ultrastructural organization of the patch matrix compartments in the human striatum

The mammalian striatum is a heterogeneous structure characterized by striosomes and matrix. The synaptic organization of the striatum has been described previously in various mammalian species including human; however, potential ultrastructural differences in striosomal organization have not been well studied. Samples (n = 7) of striatal tissue were obtained from the Maryland Brain Collection (mean age, 37.7 ± 9.4 years; and mean PMI, 5.3 ± 1.4 hours). Tissue was prepared for calbindin immunocytochemistry to identify striosomal (patch) and extrastriosomal matrix (matrix) compartments and subsequently prepared for electron microscopy. Synaptic density was determined, using stereologic methods, for all synapses combined and for various subsets of synapses such as asymmetric, symmetric, axospinous, axodendritic, and perforated in the patch and matrix of the caudate (CP, CM) and putamen (PP, PM). An ANOVA revealed significant between‐group (CP, CM, PP, PM) differences (P < 0.05) for the following types of synapses: total combined, asymmetric, axospinous, and asymmetric axospinous. Each of these four types was significantly increased in density in the CP vs the PP, whereas the matrix (CM vs PM) showed no significant differences in density in these or other synapses. In the caudate (CP vs CM), the synaptic density of the types of synapses studied did not vary significantly between the patch and the matrix. In the putamen, the matrix (PM) had higher synaptic densities than that of the patches (PP) for total synapses, symmetric dendritic, and perforated. These data show that the patch and matrix compartments are heterogeneous at the ultrastructural level, imparting another level of complexity to the striatum—a fact that should be taken into consideration when studying diseases of this brain region at the electron microscopic level. J. Comp. Neurol. 452:128–138, 2002. © 2002 Wiley‐Liss, Inc.
Screening and Brief Intervention for Alcohol Problems Among College Students Treated in a University Hospital Emergency Department

Abstract The authors evaluated a protocol to screen and provide brief interventions for alcohol problems to college students treated at a university hospital emergency department (ED). Of 2,372 drinkers they approached, 87% gave informed consent. Of those, 54% screened positive for alcohol problems (Alcohol Use Disorders Identification Test score < 6). One half to two thirds of the students who screened positive drank 2 to 3 times a week, drank 7 or more drinks per typical drinking day, or had experienced alcohol dependence symptoms within the past year. Ninety-six percent of screen-positive students accepted counseling during their ED visit. Three quarters of those questioned at 3-month follow-up reported that counseling had been helpful and that they had decreased their alcohol consumption. The prevalence of alcohol problems, high rates of informed consent and acceptance of counseling, and improved outcomes suggest that the ED is an appropriate venue for engaging students at high risk for alcohol problems.
A Recently Identified Rhinovirus Genotype Is Associated with Severe Respiratory-Tract Infection in Children in Germany

Abstract Acute respiratory infection is a significant cause of morbidity and mortality in children worldwide. Accurate identification of causative agents is critical to case management and to prioritization in vaccine development. Sensitive multiplex diagnostics provide us with an opportunity to investigate the relative contributions of individual agents andmayalso facilitate the discovery of new pathogens. Recently, application of MassTag polymerase chain reaction (PCR) to undiagnosed infuenza-like illness in New York State led to the discovery of a novel rhinovirus genotype. Here we report the investigation, by MassTag PCR, of pediatric respiratory-tract infections in Germany, studying 97 cases for which no pathogen was identified through routine laboratory evaluation. Respiratory viruses were identified in 49 cases (51%); of the 55 identified viruses, 41 (75%) were rhinoviruses. The novel genotype represented 73% of rhinoviruses and 55% of all identified viruses. Infections with the novel genotype were associated with upper-respiratory-tract symptoms but, more frequently, with bronchitis, bronchiolitis, and pneumonia.
Quantitative Relation between Conflict and Response Inhibition in the Flanker Task

The 2001 conflict monitoring hypothesis of Botvinick and colleagues posits that the amount of conflict raised by incongruent stimuli in a flanker task affects subsequent cognitive control, such as response inhibition. The present experiment yielded empirical evidence of the quantitative relation between conflict and response inhibition. Participants judged the direction of a target arrow flanked by distractor arrows presented above and below the target. The amount of conflict was manipulated by varying the distance between the target and the directional distractors. Analysis showed that response times were longer for incongruent trials than for congruent trials, and response times on incongruent trials were longer for the small distance than for the large distance conditions. In addition, the response times in congruent trials became longer as the amount of conflict in the preceding trial increased. These results are consistent with Botvinick, et al.'s hypothesis that the conflict-detection mechanism determines the amount of response inhibition depending on the amount of conflict. Responses on incongruent trials were faster and more accurate when the preceding trial was incongruent than when it was congruent, and the size of this response facilitation was not influenced by the amount of conflict. These results suggest that the conflict detection mechanism modulates the subsequent behaviors by two forms of control which are differently affected by the amount of conflict.
Forelimb anatomy and the discrimination of the predatory behavior of carnivorous mammals: The thylacine as a case study

Carnivorous mammals use their forelimbs in different ways to capture their prey. Most terrestrial carnivores have some cursorial (running) adaptations, but ambush predators retain considerable flexibility in their forelimb movement, important for grappling with their prey. In contrast, predators that rely on pursuit to run down their prey have sacrificed some of this flexibility for locomotor efficiency, in the greater restriction of the forelimb motion to the parasagittal plane. In this article, we measured aspects of the forelimb anatomy (44 linear measurements) in 36 species of carnivorous mammals of known predatory behavior, and used multivariate analyses to investigate how well the forelimb anatomy reflects the predatory mode (ambush, pursuit, or pounce‐pursuit). A prime intention of this study was to establish morphological correlates of behavior that could then be applied to fossil mammals: for this purpose, five individuals of the recently extinct thylacine (Thylacinus cynocephalus) were also included as unknowns. We show that the three different types of predators can be distinguished by their morphology, both in analyses where all the forelimb bones are included together, and in the separate analyses of each bone individually. Of particular interest is the ability to distinguish between the two types of more cursorial predators, pursuit and pounce‐pursuit, which have previously been considered as primarily size‐based categories. Despite a prior consideration of the thylacine as a “pounce‐pursuit” or an “ambush” type of predator, the thylacines did not consistently cluster with any type of predatory carnivores in our analyses. Rather, the thylacines appeared to be more generalized in their morphology than any of the extant carnivores. The absence of a large diversity of large carnivorous mammals in Australia, past and present, may explain the thylacine's generalized morphology. J. Morphol. 275:1321–1338, 2014. © 2014 Wiley Periodicals, Inc.
Correlation of adiponectin receptor expression with cytokines and insulin sensitivity in growth hormone (GH)-treated children with Prader-Willi syndrome and in non-GH-treated obese children.

CONTEXT Prader-Willi syndrome (PWS), a genetic disorder characterized by obesity in early childhood, is reported to have elevated levels of adiponectin. The effects of adiponectin are mediated by adiponectin receptors (ADIPORs) that include ADIPOR1 and ADIPOR2. There is evidence that several cytokines, including adiponectin, TNF-alpha, and IL-6, are involved in insulin sensitivity. OBJECTIVE AND METHODS We measured the relative expression of adiponectin, ADIPORs, several proinflammatory cytokines including TNF-alpha, and IL-6 expression in peripheral blood mononuclear cells (PBMCs) of children with PWS and obese comparators using real-time PCR. Their correlation with homeostasis model assessment insulin resistance index (HOMA-IR) was analyzed. PATIENTS Thirty children with PWS (median age 7.1 yr, 18 males, 12 females) that were being treated with GH and 32 obese children not receiving GH treatment (median age 9.1 yr, 15 males, 17 females) for comparison were enrolled. RESULTS The PWS children had increased expression of ADIPOR2 (P = 0.02) and decreased expression of IL-6 (P = 0.03) compared with the comparison group. Moreover, there was a significant positive correlation between the ADIPORs and TNF-alpha (ADIPOR1 vs. TNF-alpha: r = 0.66, P < 0.001 in PWS, r = 0.80, P < 0.001 in comparison group; ADIPOR2 vs. TNF-alpha: r = 0.69, P < 0.001 in comparison group). The ADIPORs in the comparison group showed significant negative correlation with HOMA-IR (ADIPOR1 vs. HOMA-IR; rho = -0.41, P = 0.02, ADIPOR2 vs. HOMA-IR; rho = -0.46, P < 0.01). CONCLUSION The results of this study showed that inflammatory cytokine expression was closely associated with the expression of the ADIPORs in the PBMCs of both the children with PWS and the comparison group. Moreover, ADIPOR2 expression was highly expressed in the PBMCs of the children with PWS. A further study on the mechanism of increased expression of ADIPOR2 and its correlation with the expression of TNF-alpha in the PBMCs using the non-GH-treated PWS and obese control will be warranted because this study compared GH-treated PWS with an obese comparator group.
Increased cortical inhibition in persons with schizophrenia treated with clozapine

It has been previously demonstrated that unmedicated persons with schizophrenia have deficits in cortical inhibition (CI) as indexed with transcranial magnetic stimulation (TMS). This inhibition is largely mediated by cortical GABAergic mechanisms. It has also been demonstrated that these inhibitory deficits may be normalized with the use of atypical antipsychotic medications. The purpose of this study, therefore, was to examine the effects of clozapine on TMS measures of CI and to compare these effects to unmedicated persons with schizophrenia and healthy subjects. We used two TMS inhibitory paradigms: short interval intra-cortical inhibition (SICI) and the cortical silent period (CSP) to evaluate CI in 10 clozapine-treated persons with schizophrenia, 6 unmedicated persons with schizophrenia and 10 healthy subjects. Clozapine-treated persons with schizophrenia had significantly longer CSPs compared with healthy subjects and unmedicated persons with schizophrenia. There were no significant differences in SICI between groups, however, the severity of psychotic symptoms was correlated with reduced SICI across all persons with schizophrenia. Our findings suggest that clozapine treatment is associated with greater CI in persons with schizophrenia and this increase may be related to potentiation of cortical GABAergic receptor mediated inhibitory neurotransmission. Our results also confirm previous findings suggesting that deficits in CI are related to the severity of psychotic symptoms in persons with schizophrenia.
Severe Maternal Morbidity During Delivery Hospitalizations.

INTRODUCTION Severe maternal morbidities include 25 complications resulting from, or exacerbated by, pregnancy. Nationally, in the last decade, these rates have doubled. OBJECTIVE This study describes trends in the rates of severe maternal morbidities at the time of hospitalization for delivery among different groups of Wisconsin women. METHODS Hospital discharge data and ICD-9-CM diagnosis and procedure codes were used to identify delivery hospitalizations and rates of severe maternal morbidity among Wisconsin women from 2000 to 2014. Subsequent analyses focused on recent years (2010-2014). Rates of severe maternal morbidity were calculated per 10,000 delivery hospitalizations for all 25 severe maternal morbidity conditions as well as 24 conditions (excluding blood transfusions). Rates and rate ratios were calculated overall and for racial/ethnic groups, age groups, public health region of residence, and hospital payer. Median hospital length of stay and median hospital charges were compared for delivery hospitalizations with increasing severe maternal morbidities. RESULTS Severe maternal morbidity rates increased 104% from 2000 to 2014 (P for trend <0.01). After excluding blood transfusions, rates increased 15% (P for trend <0.05). From 2010 to 2014, overall rates were stable over time, but varied by maternal age, race/ethnicity, payer, and public health region of residence. Median hospital charges and length of stay increased as the number of morbidities increased. CONCLUSIONS Monitoring severe maternal morbidities adds valuable information to understanding perinatal health and obstetric complications in order to identify opportunities for prevention of severe morbidities and improvements in the quality of maternity care.
Efficacy of Low-dose Ketamine as Sole Analgesic Agent in Maintaining Analgesia and Intraoperative Hemodynamics During Laparoscopic Gynecological Surgeries

Background: Ketamine, in low doses, is known to possess intense analgesic properties. The available literature shows wide variation regarding the time and dose of administration of ketamine during surgery. Aim: The aim of this study was to evaluate the effect of intraoperative administration of ketamine when used as sole analgesic in low doses, on hemodynamics and postoperative analgesia in patients undergoing laparoscopic gynecological surgery and compared on the basis of duration of surgery. Settings and Design: This prospective, observational study was conducted from July to December 2015, over a period of 6 months in a tertiary care medical college and hospital. Materials and Methods: Seventy patients between 23 and 55 years planned for laparoscopic gynecological surgery were recruited. Ketamine was given in a dose of 0.5 mg/kg preoperatively and then repeated every ½ hourly in a dose of 0.25 mg/kg throughout the surgery. Hemodynamic parameters, time to the first rescue analgesia and complications were recorded for the first 8 h. Statistical evaluation was done and result expressed as percentage. Paired t-test was employed for the comparison of numerical variables within the group. Results: Seventy percent of the patients did not require any postoperative rescue analgesia during the first 8 h after surgery. None of the patients complained of pain immediately after extubation, and 16% of the patients had minor postoperative complications. The intraoperative hemodynamic profile was significantly altered. Duration of surgery and dose of ketamine required did not affect the duration of analgesia. Conclusion: Ketamine in low dose proved to be an efficacious analgesic even in the long duration laparoscopic gynecological surgeries. It stabilizes intraoperative hemodynamics thereby reducing the requirement of other anesthetic and antihypertensive agents.
Patient satisfaction in a dental school.

PURPOSE The objective of this study was to measure patients' satisfaction with the facility, services and treatment received at a dental school clinic. METHOD The 31-item questionnaire consisted of demographic items and items in a 5-point Likert-type format addressing issues such as security, satisfaction with the facility, helpfulness of the staff, progress of treatment, fees, universal precautions taken, quality of care and treatment with dignity and compassion. The questionnaire also asked whether the patient would recommend the school to others seeking dental care. In 1997, patients completed the survey in the clinic waiting room prior to their dental appointment for that day. The survey was conducted over 3 months until 500 patients had participated, representing about 16% of the active patient population. %s of responses were computed and cross tabulations were used to compare responses based on demographics. RESULTS The most frequently reported reason for wanting to be a patient at the dental school was low cost (67%) followed by up-to-date care (19%). 45% of patients indicated that a friend, neighbour or relative referred them, followed by 34% indicating referral by a dentist. More than 90% responded positively to the items concerning issues related to treatment except for satisfaction with progress of treatment and comparison of care received at the school to care elsewhere. For these 2 items, at least 80% responded positively. 99% indicated they would recommend the dental school to others seeking dental care. CONCLUSION Results indicate that the vast majority of the patients surveyed are satisfied with the facility, services and treatment received. Comments that addressed areas of concern included the length of time to get an appointment and the length of time the appointment took, and it was recommended that the process of treatment be expedited.
Tension responses to joule temperature jump in skinned rabbit muscle fibres.

1. Joule temperature jumps (T‐jumps) from 5‐9 degrees C up to 40 degrees C were used to study the cross‐bridge kinetics and thermodynamics in skinned rabbit muscle fibres. To produce a T‐jump, an alternating current pulse was passed through a fibre 5 s after removing the activating solution (pCa congruent to 4.5) from the experimental trough. The pulse frequency was congruent to 30 kHz, amplitude less than or equal to 3 kV, and duration 0.2 ms. The pulse energy liberated in the fibre was calculated using a special analog circuit and then used for estimation of the T‐jump amplitude. 2. The T‐jump induced a tri‐exponential tension transient. Phases 1 and 2 had rate constants k1 = 450‐1750 s‐1 and k2 = 60‐250 s‐1 respectively, characterizing the tension rise, whereas phase 3 had a rate constant k3 = 5‐10 s‐1 representing tension recovery due to the fibre cooling. 3. An increase from 13 to 40 degrees C for the final temperature achieved by the T‐jump led to an increase in the amplitudes of phases 1 and 2. After T‐jumps to 30‐40 degrees C during phase 1, tension increased by 50‐80%. During phase 2 an approximately 2‐fold tension increase continued. Rate constants k1 and k2 increased with temperature and temperature coefficients (Q10) were 1.6 and 1.7, respectively. 4. To study which processes in the cross‐bridges are involved in phases 1 and 2, a series of experiments were made where step length changes of ‐9 to +3 nm (hs)‐1 (nanometres per half‐sarcomere length) were applied to the fibre 4 ms before the T‐jump. 5. After the step shortening, the rate constant of phase 1 increased, whereas its amplitude decreased compared to those without a length change. This indicates that phase 1 is determined by some force‐generating process in the cross‐bridges attached to the thin filaments. This process is, most probably, the same as that producing the early tension recovery following the length change. The enthalpy change (delta H) associated with the reaction controlling this process was estimated to be positive (15‐30 kJ mol‐1). 6. Both the rate constant k2 and the maximal tension achieved at the end of phase 2 were practically independent of the preceding length changes. This means that phase 2 is accompanied by the cross‐bridge detachment and reattachment to new sites on the thin filaments. (ABSTRACT TRUNCATED AT 400 WORDS)