Miracle-dz/UniFAR-FTUs huggingface dataset:
The morphology of collicular and retinal axons ending on small relay (W-like) cells of the primate lateral geniculate nucleus

Abstract The lateral geniculate nucleus (LGN) of every primate examined contains a set of small relay cells in addition to separate sets of magnocellular and parvocellular relay cells. These small cells receive a direct retinal projection, and an indirect retinal projection via the superior colliculus (SC). Receptive-field analyses of the small LGN cells in the bush baby, a lorisiform primate, indicate that this cell class is composed of subclasses, similar in physiology to cat W cells. In an effort to identify some of these subclasses, we have examined the morphological features of retinal and collicular axonal arbors that end on small W-like cells in the LGN of the bush baby, Galago crassicaudatus. Small cells in this species are found in a prominent pair of koniocellular (K) layers as well as the interlaminar zones (ILZs). Retinal arbors were examined by bulk iontophoretic injection of horseradish peroxidase into the optic tract. Collicular arbors were filled via iontophoretic injection of biocytin into the superficial layers of the SC. Forty-eight axon arbors were completely reconstructed and quantitatively evaluated. Our findings show that retinal and collicular axon terminals differ in morphology on the basis of a number of criteria. Our analyses also suggest that retinal axons may have a stronger influence on K cells and collicular axons have a stronger influence on ILZ cells. The ramifications of these findings are provocative since these small LGN cells are known to project directly to the cytochrome-oxidase (CO) blobs within striate cortex. This relationship suggests that CO blob cells receive complex visual input not only from magnocellular and parvocellular LGN cells, but also from small cell pathways that are differentially influenced by retinal and collicular cells.
Up‐regulation of Ca2+ influx mediated by store‐operated channels in HL60 cells induced to differentiate by 1α,25‐Dihydroxyvitamin D3

The physiologically active form of vitamin D, 1α,25‐dihydroxyvitamin D3 (1,25D3), induces promyelocytic HL60 cells to differentiate towards monocyte‐like cells. During this differentiation increased cytosolic calcium (Cai2+) and expression of surface receptors for chemotactic factors “prime” the cell for the activation of monocyte functions and the triggering of the respiratory burst pathway. We examined whether the Ca2+ influx mediated by store‐operated channels (SOC) contributed to the increased Cai2+ following exposure of HL60 cells to 10−7 M 1,25D3. Cells treated with 1,25D3 for 72 hr demonstrated a rapid transient rise in Cai2+ followed by a second, phasic, increase in Cai2+ in response to the purinergic agonist ATP. This second Cai2+ transient was blocked by Ni2+, SKF 96365, or withdrawal of extracellular Ca2++. In cells suspended in Ca2+‐free medium, peak changes (Δ) in [Ca2+]i elicited by ATP‐induced Ca2+ mobilization occurred with similar EC50 values in differentiated and vehicle (EtOH)‐treated cells; however, peak [Ca2+]i was reduced by 55% in 1,25D3‐treated cells. Decreased Ca2+ mobilization was associated with a 25–35% reduction in intracellular Ca2+ stores (determined with ionomycin). 1,25D3‐treated cells exposed to ATP or thapsigargin (Tg) in Ca2+‐free medium for 3 min with subsequent addition of 1 mM Ca2+ exhibited a respective 80% or 120% stimulation in peak [Ca2+]i compared to EtOH‐treated cells. Enhanced Ca2+ influx mediated by SOC was also seen in these cells as an increase in the rate of Mn2+ entry after exposure to ATP or Tg. At 96 hr after addition of 1,25D3, when differentiated phenotype was established, basal Ca2+i and Ca2+ entry mediated by SOC returned to control values, but Ca2+ store size remained reduced. Up‐regulation of Ca2+ influx via the SOC pathway during 1,25D3‐induced differentiation may contribute to the functional properties of the maturing monocyte, or to the resetting of molecular programs responsible for the changing phenotype. J. Cell. Physiol. 172:284–295, 1997. © 1997 Wiley‐Liss, Inc.
The management of differentiated thyroid cancer using 123I for imaging to assess the need for 131I therapy

BackgroundFollow-up of 131I whole-body scanning after 131I ablation is associated with potential stunning. Previous studies have suggested that, for scanning, 123I is more sensitive than 131I in identifying thyroid tissue, but its specificity when positive is less certain. AimThe use of 123I as an imaging agent in place of serial 131I imaging has been evaluated in the surveillance and treatment of differentiated thyroid carcinoma. ResultsA total of 186 studies in 136 patients with differentiated thyroid carcinoma were evaluated after total or near total thyroidectomy followed by 131I ablation. In 125 studies 123I scanning was negative and no 131I therapy was given; four patients were positive on 123I scanning but for other reasons no 131I therapy was given. In 48/49 patients a positive 123I scan was followed by positive 131I therapeutic uptake. Only one patient failed to show positive uptake of 131I when first treated and she subsequently demonstrated uptake on a second therapy. ConclusionHigh-dose 123I imaging is the correct predictor of the 131I post-therapy scan findings in most cases, at an administered activity that avoids stunning. As a diagnostic agent it is preferable to 131I in differentiated thyroid carcinoma.
Risk Disclosures, International Orientation, and Share Price Informativeness: Evidence from China

This paper examines the impact of textual risk disclosure on the amount of firm-specific information incorporated into share prices, as measured by stock price synchronicity. Using a sample of Chinese listed firms, we find that synchronicity is inversely associated with the extent of risk disclosure, suggesting that risk disclosure is firm specific and useful to investors. In addition, our results show that the usefulness of risk information is statistically and economically more pronounced among internationally oriented firms than domestically oriented ones. This result is consistent with the necessity for risk disclosure being more meaningful when it relates to greater uncertainty. Finally, we find that internationally oriented firms tend to disclose more risk factors than their domestically oriented counterparts. Overall, our results shed light on the current debate over whether narrative sections of annual reports convey useful information to investors.
Dasatinib inhibits the development of metastases in a mouse model of pancreatic ductal adenocarcinoma.

BACKGROUND & AIMS Pancreatic ductal adenocarcinoma (PDAC) is a highly invasive and metastatic disease for which conventional treatments are of limited efficacy. A number of agents in development are potential anti-invasive and antimetastatic agents, including the Src kinase inhibitor dasatinib. The aim of this study was to assess the importance of Src in human PDAC and to use a genetically engineered mouse model of PDAC to determine the effects of dasatinib on PDAC progression. METHODS Src expression and activity was measured by immunohistochemistry in 114 human PDACs. Targeting expression of Trp53(R172H) and Kras(G12D) to the mouse pancreas results in the formation of invasive and metastatic PDAC. These mice were treated with dasatinib, and disease progression monitored. Cell lines were derived from mouse PDACs, and in vitro effects of dasatinib assessed. RESULTS Src expression and activity were up-regulated in human PDAC and this correlated with reduced survival. Dasatinib inhibited the migration and invasion of PDAC cell lines, although no effects on proliferation were seen at concentrations that inhibited Src kinase activity. In addition, dasatinib significantly inhibited the development of metastases in Pdx1-Cre, Z/EGFP, LSL-Kras(G12D/+), LSL-Trp53(R172H/+) mice. However, there was no survival advantage in the dasatinib-treated animals owing to continued growth of the primary tumor. CONCLUSIONS This study confirms the importance of Src in human PDAC and shows the usefulness of a genetically engineered mouse model of PDAC for assessing the activity of potential antimetastatic agents and suggests that dasatinib should be evaluated further as monotherapy after resection of localized invasive PDAC.
Are Cash Flows Better Stock Return Predictors Than Profits

Although various income statement–based measures predict the cross section of stock returns, direct method cash flow measures have even stronger predictive power. We transform indirect method cash flow statements into disaggregated and more direct estimates of cash flows from operations and other sources and form portfolios on the basis of these measures. Stocks in the highest-cash-flow decile outperform those in the lowest by over 10% annually (risk adjusted). Our results are robust to investment horizons and across risk factors and sector controls. We also show that, in addition to operating cash flow information, cash taxes and capital expenditures provide incremental predictive power.
Guanylin and E. coli Heat-Stable Enterotoxin Induce Chloride Secretion through Direct Interaction with Basolateral Compartment of Rat and Human Colonic Cells

We previously detected specific binding activity of Escherichia coli heat-stable enterotoxin (ST), the guanylin exogenous ligand, in rat colonic basolateral membranes. Because guanylin circulates in the bloodstream, we tested the hypothesis that it modulates intestinal ion transport by acting on the serosal side of intestinal cells. The effects of the mucosal and serosal addition of ST and guanylin on ion transport were investigated in the rat proximal colon and in Caco-2 cells in Ussing chambers, by monitoring short-circuit current (Isc). cGMP concentration was measured in Caco-2 cells by RIA. Mucosal ST addition induced an increase in Isc in rat proximal colon consistent with anion secretion. Serosal addition induced the same effects but to a lesser extent. The electrical effects observed in Caco-2 cells paralleled those observed in rat proximal colon. A pattern similar to the electrical response was observed with cGMP concentration. Guanylin addition to either side of Caco-2 cells induced the same effects as ST, although to a lesser extent. In all conditions, the electrical effect disappeared in the absence of chloride. ST directly interacts with basolateral receptors in the large intestine inducing chloride secretion through an increase of cGMP. However, the serosal effects are less pronounced compared with those observed with mucosal addition. Guanylin shows the same pattern, suggesting that it plays a role in the regulation of ion transport in the colon, but the relative importance of serosally mediated secretion remains to be determined.
Extended treatment duration for hepatitis C virus type 1: comparing 48 versus 72 weeks of peginterferon-alfa-2a plus ribavirin.

BACKGROUND & AIMS The treatment of patients infected with hepatitis C virus (HCV) type 1 remains a challenge necessitating innovative strategies to improve treatment outcome. The extension of treatment duration beyond 48 weeks is one possible strategy to address this problem. METHODS The efficacy and safety of 48 weeks (group A, N = 230) vs 72 weeks (group B, N = 225) of treatment with pegylated-interferon-alfa-2a (180 microg/wk) plus ribavirin (800 mg/day) were studied in treatment-naive patients with HCV type 1 infection. On-treatment and sustained virologic response (SVR) 24 weeks after stopping treatment was assessed by qualitative reverse-transcription polymerase chain reaction (sensitivity 50 IU/mL). RESULTS Overall, no significant differences could be observed in the treatment outcome between both groups. End-of-treatment and SVR rates in groups A and B were 71% vs 63% and 53% vs 54%, respectively. Patients with undetectable HCV-RNA levels already at weeks 4 and 12 had excellent SVR rates ranging from 76% to 84% regardless of treatment group, whereas patients shown to be still HCV-RNA positive at week 12 achieved significantly higher SVR rates when treated for 72 instead of 48 weeks (29% vs 17%, P = .040). A particular benefit from extended treatment duration was seen in patients with low-level viremia (<6000 IU/mL) at week 12. The frequency and intensity of adverse events was similar between the 2 groups. CONCLUSIONS Extended treatment duration generally is not recommended in HCV type 1 infection and should be reserved only for patients with slow virologic response defined as HCV-RNA positive at week 12 but negative at week 24.
The Evolution of Unintentional Injury Mortality Among Elderly in Europe

Objectives: To compare cause-specific unintentional injury mortality trends among elderly (65+) in the European Union over a 10-year period. Method: Overall and cause-specific data for 23 out of the 29 EU and European Free Trade Association countries with population ≥ 1,000,000 were retrieved from the World Health Organization (WHO), and age-standardized mortality rates for the first and last 3 available years of the study period were calculated. Proportional mortality changes were estimated through linear regression. Results: Circa 1993, country-specific rates varied widely (>fourfold), but this gap is closing and a statistically significant downward trend in overall mortality is noted circa 2002, in about half of the countries. Rates from falls were reduced by 4.3%, from motor vehicle traffic by 3.1%, and from smoke, fire, and flames by 3.1%. Discussion: A large proportion of EU countries enjoys steady declining trends by major unintentional injury mortality category. Success factors and barriers underlying these benchmarking patterns should be further explored to accelerate the process of injury reduction.
Perceived challenges in university branch campuses : a case study of five Western campuses in a GCC state

This study explores the challenges perceived by senior officers in Western university branch campuses in a Gulf Cooperation Council (GCC) state and aims to provide insights into how leaders dealt with them. Thus, the study addresses four research questions. First, how campus senior officers perceive the challenges? Second, to what extent are these challenges a result of operating in a different cultural context? Third, how do senior officers deal with these challenges and maintain the quality of their home universities? Finally, how can the study findings be theorised to inform future policy and practice? As a form of transnational higher education, some of the GCC states have established branch campuses of leading Western universities to provide quality higher education to their citizens locally and build knowledge economies. Campus managers, usually from their home Western universities, face challenges related to the local cultural context. These challenges include the GCC state societal views of the campuses, the Western University approach to co-education, the GCC state development efforts to grow a Knowledge Economy through quality Western-style education, and national students’ academic preparation. This research was conducted as an exploratory qualitative multiple case study of five branch campuses. Data were collected through interviews with senior officers from the campuses and the host organization in the GCC state, and the five campuses' annual reports. Data were analysed through open and axial coding. Then thematic analysis was used to identify themes from the data. The study revealed that the major challenges for campus officers are recruiting academically strong students from the pool of citizens, and understanding the local culture and adjusting aspects of the curriculum to fit the local context especially for campuses that offer social sciences and arts programmes. Campus officers engaged in efforts to make school students more qualified for admission by the campuses, and some leaders made adjustments to the curriculum to better suit their students’ abilities.
Endoscopic ligation of esophageal varices: An endoscopist's opinion

One hundred consecutive patients with bleeding esophageal varices were treated with a new endoscopic ligating device that effects strangulation of varices using small elastic “O” rings. Treatments were continued after initial hospitalization to achieve variceal eradication. Follow‐up ranged from 6 to 26 (mean: 15) months. Bleeding was controlled until discharge from hospital or death in 18 of 21 patients who were actively bleeding at index endoscopy. Overall, 26 patients died during the study, 12 during the index hospitalization. Cause of death was organ failure in 21, exsanguination in 3, and cancer in 2. Forty‐one of 88 initial survivors experienced 72 episodes of recurrent bleeding (1 to 4 per patient). All but five rebleeds occurred before eradication. Sixty of 88 patients (68%) who survived index hospitalization had their varices eradicated. A median of 5 (1 to 12) treatments was required. Nine patients eventually had other forms of treatment for recurrent bleeding. Only 3 non‐bleeding complications resulted from 462 endoscopic treatment sessions. We conclude that endoscopic ligation controls active variceal bleeding and eradicates varices with efficacy similar to that of sclerotherapy and with minimal risk of complications.
Specificity of Cellular DNA-Binding Sites of Microbial Populations in a Florida Reservoir

The substrate specificity of the DNA-binding mechanism(s) of bacteria in a Florida reservoir was investigated in short- and long-term uptake studies with radiolabeled DNA and unlabeled competitors. Thymine oligonucleotides ranging in size from 2 base pairs to 19 to 24 base pairs inhibited DNA binding in 20-min incubations by 43 to 77%. Deoxynucleoside monophosphates, thymidine, and thymine had little effect on short-term DNA binding, although several of these compounds inhibited the uptake of the radiolabel from DNA in 4-h incubations. Inorganic phosphate and glucose-1-phosphate inhibited neither short- nor long-term binding of [3H]- or [32P]DNA, indicating that DNA was not utilized as a phosphorous source in this reservoir. RNA inhibited both short- and long-term radiolabeled DNA uptake as effectively as unlabeled DNA. Collectively these results indicate that aquatic bacteria possess a generalized nucleic acid uptake/binding mechanism specific for compounds containing phosphodiester bonds and capable of recognizing oligonucleotides as short as dinucleotides. This binding site is distinct from nucleoside-, nucleotide-, phosphomonoester-, and inorganic phosphate-binding sites. Such a nucleic acid-binding mechanism may have evolved for the utilization of extracellular DNA (and perhaps RNA), which is abundant in many marine and freshwater environments.
“ Management of land use systems for enhanced food security : conflicts , controversies and resolutions ” Social Capital and Agricultural Technology Adoption among Ethiopian Farmers

Ethiopia is one of the largest countries in Africa in terms of population and land size. Despite the efforts in recent years, food production and distribution is still a major challenge in the country. Hence, improving living conditions and food security of smallholder farmers through increasing agricultural growth remains the main objective of decision makers in the country. Many studies suggested that the way forward is fostering smallholders’ productivity, which mainly hinges on the adoption of suitable new and/or improved agricultural technologies. Understanding the determinants of agricultural technology adoption is crucial for designing appropriate policies. While previous researches highlighted the importance of socioeconomic and demographic factors, they mostly overlooked the role of social capital in technology adoption. In the Ethiopian context, however, social capital and support systems are expected to have a substantial effect on decisions related to testing and adopting new technologies since social capital is capable of creating collective action, relaxing financial constraints, reducing transaction cost, and disseminating information among farmers. This study uses socio-economic data of 404 Ethiopian farming households living in a 200 km radius around Awassa town. First, it describes the characteristics of farming households with a special focus on different social capital indicators and the agricultural innovations available in the communities. Second, using a probit model and other regression analysis methods like OLS, it assesses the effects of social capital on the adoption of purchasable agricultural technologies (e.g. fertiliser, chemicals, etc.) and non-purchasable improved practices of land-water resources management (e.g. terracing, crop rotation, etc). The findings of the study are expected to clearly identify social capital indicators relevant for the research area and estimate the effects of social capital on technology adoption. This provides the empirical foundation based on which relevant policy options for smallholders’ livelihood improvement can be recommended.
ERK-1/2 and p38 in the regulation of hypertrophic changes of normal articular cartilage chondrocytes induced by osteoarthritic subchondral osteoblasts.

OBJECTIVE Previous studies have shown the influence of subchondral bone osteoblasts (SBOs) on phenotypical changes of articular cartilage chondrocytes (ACCs) during the development of osteoarthritis (OA). The molecular mechanisms involved during this process remain elusive, in particular, the signal transduction pathways. The aim of this study was to investigate the in vitro effects of OA SBOs on the phenotypical changes in normal ACCs and to unveil the potential involvement of MAPK signaling pathways during this process. METHODS Normal and arthritic cartilage and bone samples were collected for isolation of ACCs and SBOs. Direct and indirect coculture models were applied to study chondrocyte hypertrophy under the influence of OA SBOs. MAPKs in the regulation of the cell-cell interactions were monitored by phosphorylated antibodies and relevant inhibitors. RESULTS OA SBOs led to increased hypertrophic gene expression and matrix calcification in ACCs by means of both direct and indirect cell-cell interactions. In this study, we demonstrated for the first time that OA SBOs suppressed p38 phosphorylation and induced ERK-1/2 signal phosphorylation in cocultured ACCs. The ERK-1/2 pathway inhibitor PD98059 significantly attenuated the hypertrophic changes induced by conditioned medium from OA SBOs, and the p38 inhibitor SB203580 resulted in the up-regulation of hypertrophic genes in ACCs. CONCLUSION The findings of this study suggest that the pathologic interaction of OA SBOs and ACCs is mediated via the activation of ERK-1/2 phosphorylation and deactivation of p38 phosphorylation, resulting in hypertrophic differentiation of ACCs.