Miracle-dz/UniFAR-FTUs huggingface dataset:
Macrophage galactose‐type C‐type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation

Molecular markers, especially surface markers associated with type II, cytokine‐dependent, alternatively activated macrophages (aaMF), remain scarce. Besides the earlier documented markers, macrophage mannose receptor and arginase 1, we demonstrated recently that murine aaMF are characterized by increased expression of found in inflammatory zone 1 (FIZZ1) and the secretory lectin Ym. We now document that expression of the two members of the mouse macrophage galactose‐type C‐type lectin gene family (mMGL1 and mMGL2) is induced in diverse populations of aaMF, including peritoneal macrophages elicited during infection with the protozoan Trypanosoma brucei brucei or the Helminth Taenia crassiceps and alveolar macrophages elicited in a mouse model of allergic asthma. In addition, we demonstrate that in vitro, interleukin‐4 (IL‐4) and IL‐13 up‐regulate mMGL1 and mMGL2 expression and that in vivo, induction of mMGL1 and mMGL2 is dependent on IL‐4 receptor signaling. Moreover, we show that expression of MGL on human monocytes is also up‐regulated by IL‐4. Hence, macrophage galactose‐type C‐type lectins represent novel surface markers for murine and human aaMF.
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or a review of the full text. Article exclusions were due to not meeting the inclusion criteria; in particular, the articles were not empirical qualitative research. For example one promising article was titled ‘Exploration of Australian and New Zealand indigenous people’s spirituality and mental health’ [28]. The abstract stated that the authors were going to explore the concept of spirituality and use mental health in a practice setting. However, the article did not use qualitative methodology, was not empirical research and did not include Indigenous people’s perspectives on the concept of spirituality and well-being, and hence was excluded. No articles that met the inclusion criteria were excluded upon subsequent review. While a meta-analysis has a robust methodology with clearly defined processes and steps [29], the qualitative meta-synthesis does not have a similar defined pathway. Some authors argue the value of being inclusive in decision-making surrounding which studies to include in a meta-synthesis [24]. Appraisement of the quality of qualitative articles for inclusion in a meta-synthesis is a contentious issue [22]. Checklists to determine the quality of qualitative studies have been used by some researchers [22,30,31] while other researchers do not include a quality appraisement of qualitative studies in their meta-synthesis [24,25]. We aimed for inclusivity rather than subjecting the studies to rigorous quality analysis, as would occur in a meta-analysis. We determined the suitability of the identified studies based on whether qualitative methods were used for data collection and analysis and the findings were reported in a descriptive qualitative style.
Neuraxial Medication Delivery: The Development and Maturity of a Concept for Treating Chronic Pain of Spinal Origin

Study Design. A literature review and synthesis were performed. Objective. To summarize the history, use, and innovation related to neuraxial drug delivery for the treatment of intractable back pain. Summary of Background Data. The discovery of opioid receptors in the early 1970s provided a rational basis for the delivery of opioid drugs intraspinally. Epidural or intrathecal infusions deliver drugs directly to opioid receptors, limit systemic exposure, and by decreasing the opioid dosage required for pain relief, generally reduce side effects. The benefits of short-term spinal analgesia led to investigation of longer-term continuous subarachnoid opioid infusions for the management of both cancer pain and noncancer pain, such as that of spinal origin. Methods. 650 650 650 Results. Unique features of this article include an updated pain continuum, updated indications for intrathecal therapy, a detailed comparison of trial techniques, a detailed comparison of the advantages of different types of pumps, a synopsis of troubleshooting for inadequate efficacy, and an updated statement regarding intrathecal pumps and radiologic procedures, including MRI scanning. Some challenges remain. Large-scale well-controlled studies could answer some perplexing questions regarding efficacy in patients with noncancer or neuropathic pain. Patient selection criteria undoubtedly will be refined and validated as more patients are treated. In addition, further investigation of specifically targeted medications or drug combinations for intraspinal use could increase efficacy, reduce side effects, and expand indications. Conclusions. Intraspinal medication delivery has become an effective technique for control of intractable pain in appropriately selected patients seen by spine surgeons.
Effects of systemic cell-mediated immunity on vaginal candidiasis in mice resistant and susceptible to Candida albicans infections

Studies to date with CBA/J mice suggest a limited role for systemic cell-mediated immunity (CMI) against vaginal Candida albicans infections. The results of the present study show that preinduced Candida-specific systemic CMI was equally nonprotective against C. albicans vaginal infections in mice with high (BALB/cJ), low (DBA/2), or intermediate (CBA/J) resistance to C. albicans infections. Similarly, the locally acquired partial protection against a second C. albicans vaginal infection was equally observed with BALB/cJ, DBA/2, and CBA/J mice. These results indicate that observations made previously with CBA/J mice were not murine strain specific and provide additional support for the hypothesis that systemic CMI does not represent a dominant host defense mechanism at the vaginal mucosa.
The costs of repression: a meta-analysis on the relation between repressive coping and somatic diseases.

OBJECTIVE When Freud introduced the term repression, he stated its pathogenic potential. Since then, this notion was adapted and continued to date. Surprisingly, there is no attempt to synthesize research on the effect of repressive coping on somatic diseases quantitatively. The current study closes this gap and examines the association between repressive coping as operationalized by Weinberger, Schwartz, and Davidson (1979) and the incidence of cancer, cardiovascular diseases, asthma, and diabetes by applying meta-analytic techniques. METHOD An extensive literature search identified 22 studies that met all inclusion criteria and assessed a total of 6,775 participants. The study set was analyzed both as a whole sample (k = 22 studies) and disease-specific (k = 2-10 studies; no study found for diabetes). RESULTS The results of the meta-analysis indicate a higher risk for repressive copers to suffer from one of the investigated diseases, especially cancer (logOdds after diagnosis: 0.41; 95% confidence interval, 0.09-0.73, p = .012) and hypertension (logOdds: 0.59; 95% confidence interval, 0.32-0.86, p < .0001). After application of a continuity correction due to a missing control group the results for coronary heart disease imply an increased risk for nonrepressors to be affected. Results for cardiovascular diseases in general, heart attack, and asthma did not reach significance. CONCLUSIONS The current meta-analysis revealed significant associations between repressive coping, cancer, and cardiovascular diseases, especially hypertension. These results add to the notion of repressive coping as a consequence of cancer as well as to its important role for the issue of hypertension.
[Effect of rhTFAR19 protein on cell cycle and apoptosis of MCF-7 induced by gamma-ray].

OBJECTIVE To investigate the effect of protein product of TFAR19, an apoptosis-related gene previously cloned from leukemia cell line TF-1 cultured in medium deprived of growth factor, on cell cycle and apoptosis of human breast cancer induced by gamma-ray. METHODS Cell morphology, fluorescence microscopy, laser scanning confocal microscopy, single-cell gel electrophoresis (SCGE) and flow cytometry (FCM) were applied to study cell cycle and apoptosis of breast cancer cell line MCF-7. RESULTS rhTFAR19 protein itself had no effect on cell cycle arrest and apoptosis of MCF-7 cell induced by gamma-ray. When MCF-7 cells were pretreated with 10 micrograms/ml rhTFAR19 and then irradiated with 12 Gy gamma-ray, cell apoptosis was observed. It was characterized morphologically by chromatin condensation, nucleoar disintegration, formation of apoptotic bodies, and typical "cornet" on SCGE. CONCLUSION TFAR19 protein has no apparent effect on MCF-7 cell cycle induced by gamma-ray. However, it synergizes with gamma-radiation in the induction and enhancement of apoptosis.
Programmed cell death in bovine mammary tissue during lactation and involution

Cessation of milk removal causes mammary tissue involution, which in rodents is characterized by extensive tissue degeneration and loss of the majority of luminal epithelial cells by apoptosis. In contrast, bovine mammary tissue shows little histological evidence of tissue remodelling between lactations. In this study, we combined histology with molecular biology to examine the cellular and molecular changes in bovine mammary tissue on cessation of milking. Oligonucleosomal laddering of genomic DNA extracted from lactating tissue indicated that a proportion of cells were dying by apoptosis. This was confirmed by terminal deoxynucleotide transferase‐mediated deoxyuridine nick end‐labelling of apoptotic cells in lactating tissue sections (TUNEL). One week after cessation of milking, alpha‐lactalbumin and alpha S1‐casein messenger RNA (mRNA) abundance had decreased by 99 and 85%, respectively, whereas lactoferrin mRNA had increased 20‐fold. Drying off was also accompanied by an increase in oligonucleosomal laddering of genomic DNA, and by an increase in the proportion of TUNEL‐positive cells, which were localized preferentially in regions where alveolar structure had deteriorated. Therefore, termination of lactation was associated with partial loss of the mammary cell population and dedifferentiation of the remainder.
Anthracycline-induced cardiotoxicity in the chemotherapy treatment of breast cancer: Preventive strategies and treatment

Anthracyclines are highly effective chemotherapeutic agents, used for a wide variety of malignancies. Cardiotoxicity is a well-recognized side effect of anthracycline therapy that limits the total amount of drug administered and can cause heart failure in some patients. Most experimental data support oxidative stress as the etiology of anthracycline-induced cardiotoxicity. The objective of this paper was to provide a review of the clinical classification, risk factors, monitoring and prevention of anthracycline-induced cardiotoxicity in patients with breast cancer.
Undernutrition and the incidence of tuberculosis in India: national and subnational estimates of the population-attributable fraction related to undernutrition.

BACKGROUND India has the largest global burden of tuberculosis (TB)-related morbidity and mortality as well as undernutrition. Undernutrition impairs cell-mediated immunity, is a risk factor for the development of TB, and has the largest potential impact on the incidence of TB in countries with a high burden of TB. METHODS We refined the national estimate of the population-attributable fraction (PAF) for undernutrition in India to report the first subnational estimates, and stratified these further for age, gender, residence, caste and socioeconomic status. We also compared the PAF related to undernutrition in India with that in 15 other countries with a high burden of TB. We used data on body mass index (BMI) from the National Family Health Survey-3 (NFHS-3), as well as risk estimates for a low BMI from a recently published population-based study which had controlled for several confounders. RESULTS The overall prevalence of undernutrition in the age group of 15-49 years was 35.6% among women and 34.2% among men. About half (55.4%; 95% CI 27.4- 75.9) of all cases of active TB among women and 54.4% (95% CI 26.5-75.2) of all cases among men were attributable to undernutrition. In the age group of 15-19 years, the PAFs for undernutrition were 62% and 67% among women and men, respectively. The PAF of undernutrition was higher in rural areas, in scheduled castes, scheduled tribes and other backward classes, and in the lower quintiles of the wealth index. The PAF of undernutrition exceeded 50% in most states, and the largest PAFs were seen among women of scheduled tribes in central India. Among countries with a high burden of TB, India had the highest PAF related to undernutrition. CONCLUSION Addressing the problem of endemic undernutrition among adolescents and adults in India could complement the current TB control strategy based on case management, and help reduce the incidence of TB in India in line with global targets.
Correlates of heart rate recovery over 20 years in a healthy population sample.

INTRODUCTION Slow HR recovery (HRR) from a graded exercise treadmill test (GXT) is a marker of impaired parasympathetic reactivation that is associated with elevated mortality. Our objective was to test whether demographic, behavioral, or CHD risk factors during young adulthood were associated with the development of slow HRR. METHODS Participants from the Coronary Artery Risk Development in Young Adults study underwent symptom-limited maximal GXT using a modified Balke protocol at baseline (1985-1986) and 20-yr follow-up (2005-2006) examinations. HRR was calculated as the difference between peak HR and HR 2 min after cessation of the GXT. Slow HRR was defined as 2-min HRR <22 beats·min(-1). RESULTS In 2730 participants who did not have slow HRR at baseline, mean ± SD HRR was 44 ± 11 beats·min(-1) at baseline and declined to 40 ± 12 beats·min(-1) in 2005-2006; slow HRR developed in 5% (n = 135) of the sample by 2005-2006. Female sex, black race, fewer years of education, obesity, cigarette smoking, higher depressive symptoms, higher fasting glucose, hypertension, metabolic syndrome, and physical inactivity and low fitness were each associated with incident slow HRR. In a multivariable model, higher body mass index, larger waist, low education, fasting glucose, and current smoking remained significantly associated with incident slow HRR. Increasing body mass index (per SD higher) during follow-up and incident hypertension, diabetes, and metabolic syndrome (in the subsets of participants who were free from those conditions at baseline) were each associated with significantly elevated odds of incident slow HRR. CONCLUSIONS On average, HRR declines with aging; however, the odds of having slow HRR in early middle age is significantly associated with traditional CHD risk factors.
Michael Porter’s cluster theory as a local and regional development tool: The rise and fall of cluster policy in the UK

There has been much written on industrial agglomeration, but it is Michael Porter’s cluster theory, above all others, which has come to dominate local and regional economic development policy. His work has been adopted by the OECD, EU, national and local governments the world over. He and his consultancy group have led reviews of national economic growth strategies in dozens of countries. This rise to prominence, however, is in the face of widespread critique from academics. Cluster theory’s theoretical foundations, its methodological approach and practical implementation have all been unpicked, leading some to label little more than a successful brand riding the wave of new regionalist fashions. Despite libraries of incredibly useful books and articles on clusters, there remains an absence of work which interrogates the translation of clusters into, and then through local and national policy. The aim of this article is to go some way to remedying the situation by examining the influence of Porter’s cluster theory charted through an examination of UK regional development policy in the 1990s and 2000s. To help map the journey of clusters into and through UK economic development policy actor-network theory is adopted as an explanatory framework.
Preliminary assessment of the safety and immunogenicity of live oral cholera vaccine strain CVD 103-HgR in healthy Thai adults

A single dose (5 x 10(8) organisms) of attenuated A- B+ Vibrio cholerae classical Inaba recombinant vaccine strain CVD 103-HgR or placebo was administered to 24 healthy young Thai adults in a randomized, placebo-controlled, double-blind trial of safety and immunogenicity. None of the volunteers experienced untoward reactions. The vaccine strain was recovered from 2 of 12 vaccines. The vibriocidal antibody response (the best immunological correlate of protection) was good: 11 of 12 vaccinees (92%) manifested significant serotype-homologous Inaba antibody rises with a peak reciprocal geometric mean titer (RGMT) postvaccination of 3,417; 9 of 12 exhibited significant serotype-heterologous Ogawa antibody rises (prevaccination RGMT, 180; peak RGMT, 2,874). Nine of 12 vaccinees had significant rises in serum antitoxin. None of the controls exhibited rises in vibriocidal or antitoxic antibody. This preliminary study further confirms the safety and immunogenicity of CVD 103-HgR live oral cholera vaccine and paves the way for larger community studies of this candidate cholera vaccine.
hsa-miR29b, a critical downstream target of non-canonical Wnt signaling, plays an anti-proliferative role in non-small cell lung cancer cells via targeting MDM2 expression

Summary In non-small cell lung cancer cell lines, activation of &bgr;-catenin independent signaling, via Wnt7a/Frizzled9 signaling, leads to reversal of cellular transformation, reduced anchorage-independent growth and induction of epithelial differentiation. miRNA expression profiling on a human lung adenocarcinoma cell line (A549) identified hsa-miR29b as an important downstream target of Wnt7a/Frizzled9 signaling. We show herein that hsa-miR29b expression is lost in non-small cell lung cancer (NSCLC) cell lines and stimulation of &bgr;-catenin independent signaling, via Wnt7a expression, in NSCLC cell lines results in increased expression of hsa-miR29b. Surprisingly, we also identify specific regulation of hsa-miR29b by Wnt7a but not by Wnt3, a ligand for &bgr;-catenin-dependent signaling. Interestingly, knockdown of hsa-miR29b was enough to abrogate the tumor suppressive effects of Wnt7a/Frizzled9 signaling in NSCLC cells, suggesting that hsa-miR29b is an important mediator of &bgr;-catenin independent signaling. Finally, we show for the first time that hsa-miR29b plays an important role as a tumor suppressor in lung cancer by targeting murine double mutant 2 (MDM2), revealing novel nodes for Wnt7a/Frizzled9-mediated regulation of NSCLC cell proliferation.
Pantoea agglomerans, a Plant Pathogen Causing Human Disease

ABSTRACT We present 53 pediatric cases of Pantoea agglomerans infections cultured from normally sterile sites in patients seen at a children's hospital over 6 years. Isolates included 23 from the bloodstream, 14 from abscesses, 10 from joints/bones, 4 from the urinary tract, and 1 each from the peritoneum and the thorax. P. agglomerans was most associated with penetrating trauma by vegetative material and catheter-related bacteremia.
Hygienic quality and composition of raw sheep's bulk milk samples on selected Slovak farms during year 2018

At the control of raw ewe's milk (REM) quality is a major microbiological criterion to the total bacterial count (TBC). The aim of our work was to determine the incidence of technologically important species of microorganisms in REM in Slovak Republic. At the monitored 28 ewe´s farms, we took bulk milk samples from evening or morning milking in spring, summer and autumn during year 2018. We analyzed nutrients (fat, protein, lactose and urea) and somatic cell count (SCC). We established technologically important microorganisms (MO) of psychotrophic MO, coliform MO, thermoresistant MO, spore-forming anaerobic MO. We have found a gradual increase in milk components, except for lactose, which is apparently related to the increasing cont of somatic cells during the milking period. We found that the TBC in raw sheep's milk complied an average of 132 x 103 CFU.mL-1 per spring (min 34 x 103 CFU.mL-1, max 501 x 103 CFU.mL-1), 300 x 103 CFU.mL-1 in summer (min. 31 x 103 CFU.mL-1, max 640 x 103 CFU.mL-1) and in autumn with an average value of 147 x 103 CFU.mL-1 (min 52 x 103 CFU.mL-1, max 276 x 103 CFU.mL-1). The enormous occurrence of psychrotrophic bacteria was found in one farm in northern Slovakia during spring and summer, in the summer we increased our number to 3 farms, in the autumn of 2 farms. At the other farms we evaluated the average value of 12 x 103 CFU.mL-1 per spring and 28 x 103 CFU.mL-1 in summer, 130.5 x 103 CFU.mL-1 in the autumn. The count of thermoresistent MO achieved 57 CFU.mL-1 per spring, 15 CFU.mL-1 in summer and 33 CFU.mL-1 in the autumn. The presence of spore-forming anaerobic MO in raw ewe´s milk was found during spring at six farms out of 15, but in the summer at just one in 9, in the autumn two farms.
A Novel Emergency Medical Services–Based Program to Identify and Assist Older Adults in a Rural Community

Rural‐dwelling older adults experience unique challenges related to accessing medical and social services. This article describes the development, implementation, and experience of a novel, community‐based program to identify rural‐dwelling older adults with unmet medical and social needs that leveraged the existing emergency medical services (EMS) system. The program specifically included geriatrics training for EMS providers; screening of older adult EMS patients for falls, depression, and medication management strategies by EMS providers; communication of EMS findings to community‐based case managers; in‐home evaluation by case managers; and referral to community resources for medical and social interventions. Measures used to evaluate the program included patient needs identified by EMS or the in‐home assessment, referrals provided to patients, and patient satisfaction. EMS screened 1,231 of 1,444 visits to older patients (85%). Of those receiving specific screens, 45% had fall‐related, 69% medication management–related, and 20% depression‐related needs identified. One hundred and seventy‐one eligible EMS patients who could be contacted accepted the in‐home assessment. Of the 153 individuals completing the assessment, 91% had identified needs and received referrals or interventions. This project demonstrated that screening by EMS during emergency care for common geriatric syndromes and linkage to case managers is feasible in this rural community, although many will refuse the services. Further patient evaluations by case managers, with subsequent interventions by existing service providers as required, can facilitate the needed linkages between vulnerable rural‐dwelling older adults and needed community‐based social and medical services.
The origin of human triploids

the data using a maximum‐likelihood method was that 66.4 yo of the triploids were the result of dispermy, 23.6 % the result of fertilization of a haploid ovum by a diploid sperm formed by failure of the first meiotic division in the male and 10 % the result of a diploid egg formed by failure of the first maternal meiotic division. The possible sources of error inherent in the technique me reviewed and our results compared with previously published data.