Miracle-dz/UniFAR-FTUs huggingface dataset:
Macular Pigment Optical Density and Its Relationship with Refractive Status and Foveal Thickness in Chinese School-aged Children

Purpose: To investigate macular pigment optical density (MPOD) and its relationship with refractive status and foveal thickness in Chinese school-aged children. Methods: Ninety-four healthy Chinese children, 6 to 12 years old, were recruited to the study. MPOD was measured with a heterochromatic flicker photometer (HFP), and foveal thickness, including both minimum and central foveal thicknesses (MFT and CFT, respectively), were measured by optical coherence tomography (OCT) with fast macular map scan. A noncontact tonometer was used to measure intraocular pressure (IOP) followed by determination of the refraction using an autorefractor after cycloplegia. Information on body mass index (BMI) was obtained. The correlation between MPOD values and foveal thickness, spherical equivalent (SE) refraction, IOP, BMI, sex, and age was statistically analyzed using SAS 8.2 statistical software. Results: The MPOD in examined school-aged children was 0.56 ± 0.25, without any significant difference between boys and girls (p = 0.12). MPOD showed no significant association with age, BMI, IOP, SE, MFT, or CFT. In the myopia group, however, there was an inverse relationship between MPOD and MFT (R =–0.66, p = 0.028) and a positive relationship between MPOD and CFT (R = 0.67, p = 0.025). Conclusions: MPOD was inversely related to MFT and positively related to CFT in Chinese school-age children with low-to-moderate myopia. MPOD showed no significant association with age, BMI, IOP, SE or foveal thickness.
Sex differences in the thermoregulation and evaporative water loss of a heterothermic bat, Lasiurus cinereus, during its spring migration

SUMMARY This study quantifies sex differences in thermoregulation and water loss of a small (20-35 g) insectivorous heterothermic mammal, the hoary bat Lasiurus cinereus, during its spring migration. We measured body temperature, metabolic rate and evaporative water loss, and calculated wet thermal conductance, for bats exposed to air temperatures ranging from 0 to 40°C for periods of 2-5 h. Pregnant females maintained normothermic body temperatures (35.7±0.7°C; mean ± s.e.m.) independent of air temperature. In contrast, males became torpid during the majority (68%) of exposures to air temperatures <25°C. The thermal neutral zone (TNZ) ranged between approximately 30°C and 34°C in both sexes and, within the TNZ, females had lower mass-specific metabolic rates (6.1±0.2 mW g-1) than males (9.0±0.9 mW g-1). Wet thermal conductance values in torpid bats (0.7±0.5 mW g-1 deg.-1) were lower than those of normothermic individuals (1.1±0.3 mW g-1 deg.-1). Mass-specific rates of evaporative water loss in males were consistently higher than in females at most air temperatures and rates of water loss in torpid bats were 63±6% of normothermic values. These results suggest that male and pregnant female L. cinereus employ different thermoregulatory strategies during their spring migration. Females defend normothermic body temperatures, presumably to expedite embryonic growth, while males use torpor, presumably to minimize energy and water deficits. These variable thermoregulatory strategies may reflect continental differences in the summer distribution of the sexes.
Maximum Finger Force Prediction Using a Planar Simulation of the Middle Finger

Maximum isometric finger-grip forces were predicted using a biomechanical model for plane motion of the middle finger. In the course of this study, mathematical representations of tendon displacement, the moment arm of tendon at the finger joints and muscle force-length relationship were investigated. The information gathered was applied to the model to estimate the maximum grip force of the middle finger gripping cylinders of different sizes. Muscle force per unit physiological cross-section area of 30 N/cm2 resulted in good agreement with measured force. However, for finger postures having an acute proximal interphalangeal joint angle, the estimated force was greater than that measured. Various joint angles were applied to the model to simulate the wrist and finger postures not limited to the cylinder grip. In general, the finger force was greatest with the wrist in its extended position and at acute flexion of the proximal interphalangeal joint. The maximum finger force occurred at reduced metacarpophalangeal joint angles as the wrist joint changed from an extended position to a flexed one. It is also postulated that muscle force-length relationship is an important factor in muscle force predictions. The data obtained by this research are useful for the design of handles and the current model is applicable to the analysis of hand postures for workers using hand tools.
High‐Resolution Insight into Materials Criticality: Quantifying Risk for By‐Product Metals from Primary Production

Many advanced energy and environmentally relevant technologies rely on metals that have been identified as critical, or whose availability may be limited. Several of these elements are produced mostly as by‐products of mining other base metals (carriers). This by‐product dependence has been proposed as a significant supply‐risk indicator by the materials criticality community. This article provides new quantitative evidence that, in several cases, by‐product metals’ availability may not be directly limited by carrier supply. We perform an assessment based on characteristics essential to by‐product metals, including physical concentration, market value of metals, and extraction technology efficiency. We analyze 40 carrier/by‐product pairs and identify five ‘high‐by‐product’ pairs. We assess the supply responsiveness of these metals. Our analysis suggests that rather than limited primary production of carrier, lack of incentive for improving recovery efficiency may limit availability of the by‐product. This behavior is found in the zinc‐indium and copper‐selenium systems. For germanium, on the other hand, we instead propose influence from the by‐product market itself leading to price inelasticity of supply. As a complement to other quantitative methods developed for material systems, such as material flow analysis, we provide an essential technoeconomic analysis of the by‐product metals problem by employing cluster analysis and econometric modeling. This approach provides insight into supply‐risk mitigation strategies related to extraction efficiency and supply‐chain structure.
Purification and characterization of a novel secondary fimbrial protein from Porphyromonas gulae

Background : Porphyromonas gulae are black-pigmented anaerobic bacteria isolated from the gingival sulcus of various animal hosts and are distinct from Porphyromonas gingivalis originating in humans. We previously reported the antigenic similarities of 41-kDa fimbriae between P. gulae ATCC 51700 and P. gingivalis ATCC 33277. In this study, to clarify the presence of another type of fimbriae of P. gulae, we have purified and characterized the secondary fimbrial protein from P. gulae ATCC 51700. Methods : The secondary fimbrial protein was purified from P. gulae ATCC 51700 using an immunoaffinity column coupling with antibodies against the 41-kDa fimbrial protein. The expression of fimbriae on the cell surface of P. gulae ATCC 51700 was investigated by transmission electron microscopy. The N-terminal amino acid sequence was determined by an amino acid sequencer system. Results : The molecular mass of this protein was approximately 53-kDa, as estimated by SDS-PAGE. The polyclonal antibodies against the 53-kDa protein did not react with the 41-kDa fimbrial protein of P. gulae ATCC 51700. Immunogold electron microscopy revealed that anti-53-kDa fimbrial serum bound to fimbria on the cell surface of P. gulae ATCC 51700. The amino acid sequence of the N-terminal 15 residues of the 53-kDa fimbrial protein showed only 1 of 15 residues identical to the 41-kDa fimbrial protein. Conclusion : The 53-kDa fimbriae are different in molecular weight and antigenicity from the 41-kDa fimbrial protein of P. gulae ATCC 51700. These results clearly suggest that the 41-kDa and the 53-kDa fimbriae are distinct types of fimbriae expressed simultaneously by this organism.
Molecular analysis of thiopurine S‐methyltransferase alleles in Taiwan aborigines and Taiwanese

Background: Thiopurine S‐methyltransferase (TPMT) is a cytosolic enzyme involved in the metabolism of these thiopurine drugs. Methylation of thiopurine drugs by TPMT competes with the formation of their active 6‐thioguanine nucleotide metabolite, thereby potentially modulating the therapeutic and toxic effects of these drugs.
Binding and surface exposure characteristics of the gonococcal transferrin receptor are dependent on both transferrin-binding proteins

Neisseria gonorrhoeae is capable of iron utilization from human transferrin in a receptor-mediated event. Transferrin-binding protein 1 (Tbp1) and Tbp2 have been implicated in transferrin receptor function, but their specific roles in transferrin binding and transferrin iron utilization have not yet been defined. We utilized specific gonococcal mutants lacking Tbp1 or Tbp2 to assess the relative transferrin-binding properties of each protein independently of the other. The apparent affinities of the wild-type transferrin receptor and of Tbp1 and Tbp2 individually were much higher than previously estimated for the gonococcal receptor and similar to the estimates for the mammalian transferrin receptor. The binding parameters of both of the mutants were distinct from those of the parent, which expressed two transferrin-binding sites. Tbp2 discriminated between ferrated transferrin and apotransferrin, while Tbp1 did not. Results of transferrin-binding affinity purification, and protease accessibility experiments were consistent with the hypothesis that Tbp1 and Tbp2 interact in the wild-type strain, although both proteins were capable of binding to transferrin independently when separated in the mutants. The presence of Tbp1 partially protected Tbp2 from trypsin proteolysis, and Tbp2 also protected Tbp1 from trypsin exposure. Addition of transferrin to wild-type but not mutant cells protected Tbp1 from trypsin but increased the trypsin susceptibility of Tbp2. These observations indicate that Tbp1 and Tbp2 function together in the wild-type strain to evoke binding conformations that are distinct from those expressed by the mutants lacking either protein.
Jatropha curcas methyl ester oil obtaining as vegetable insulating oil

This paper presents a new vegetable oil that is jatropha curcas seeds oil as a substitute for mineral oil. This product has several advantages among which the fact that it is non-food crops and may be grown on low fertile soils and areas where annual rainfall levels are significantly lower than those required by other plants used to extract other vegetable oils such as rape-seeds, sunflower, corn, soybeans, grape-seeds and sesame. The considered natural ester oil is processed by alkali base catalyzed esterification process using potassium hydroxide (KOH) to produce jatropha curcas methyl ester oil (JMEO). The physicochemical (water content, relative density, viscosity, acidity, visual examination, color, iodine number, corrosivity, flash point, pour point) and electrical (breakdown voltage under ac, dc and impulse voltages) properties of JMEO are presented and discussed. A gas chromatography (GC) analysis is also achieved to identify the methyl ester components in JMEO. The breakdown voltage of JMEO is compared to that of mineral oil (MO). It is found that the average breakdown voltage of JMEO and MO under different voltage waveforms are too close.
A Bacterial Transgene for Catalase Protects Translation of D1 Protein during Exposure of Salt-Stressed Tobacco Leaves to Strong Light[OA]

During photoinhibition of photosystem II (PSII) in cyanobacteria, salt stress inhibits the repair of photodamaged PSII and, in particular, the synthesis of the D1 protein (D1). We investigated the effects of salt stress on the repair of PSII and the synthesis of D1 in wild-type tobacco (Nicotiana tabacum ‘Xanthi’) and in transformed plants that harbored the katE gene for catalase from Escherichia coli. Salt stress due to NaCl enhanced the photoinhibition of PSII in leaf discs from both wild-type and katE-transformed plants, but the effect of salt stress was less significant in the transformed plants than in wild-type plants. In the presence of lincomycin, which inhibits protein synthesis in chloroplasts, the activity of PSII decreased rapidly and at similar rates in both types of leaf disc during photoinhibition, and the observation suggests that repair of PSII was protected by the transgene-coded enzyme. Incorporation of [35S]methionine into D1 during photoinhibition was inhibited by salt stress, and the transformation mitigated this inhibitory effect. Northern blotting revealed that the level of psbA transcripts was not significantly affected by salt stress or by the transformation. Our results suggest that salt stress enhanced photoinhibition by inhibiting repair of PSII and that the katE transgene increased the resistance of the chloroplast's translational machinery to salt stress by scavenging hydrogen peroxide.
When Is Being Known or Adored by Romantic Partners Most Beneficial? Self-Perceptions, Relationship Length, and Responses to Partner's Verifying and Enhancing Appraisals

An experiment tested the hypothesis that relationship length moderates preferences for both verifying and enhancing appraisals from romantic partners, particularly for people with negative self-perceptions. One hundred and three romantically involved couples participated in this research. Participants with both negative and positive self-perceptions were randomly assigned to receive either verifying or enhancing feedback ostensibly created through comparison of their self-ratings and their partner's appraisals. The critical test was for those with negative self-ratings who received verifying feedback. For these participants, results revealed that those in longer relationships felt their partners were seeing the best in them more than did those in shorter relationships, whereas the opposite pattern of results was observed for those who were enhanced. Individuals with negative self-ratings who were verified also reported greater feelings of intimacy in the relationship when in long-term relationships. The importance of relationship length in moderating responses to partner's appraisals is discussed.
The Relationship Between Geographic Access to Plastic Surgeons and Breast Reconstruction Rates Among Women Undergoing Mastectomy for Cancer

Introduction Despite a national health care policy requiring payers to cover breast reconstruction, rates of postmastectomy reconstruction are low, particularly among minority populations. We conducted this study to determine if geographic access to a plastic surgeon impacts breast reconstruction rates. Methods Using 2010 inpatient and ambulatory surgery data from 10 states, we identified adult women who underwent mastectomy for breast cancer. Data were aggregated to the health service area (HSA) level and hierarchical generalized linear models were used to risk-standardize breast reconstruction rates (RSRR) across HSAs. The relationship between an HSA's RSRR and plastic surgeon density (surgeons/100,000 population) was quantified using correlation coefficients. Results The final cohort included 22,997 patients across 134 HSAs. There was substantial variation in plastic surgeon density (median, 1.4 surgeons/100,000; interquartile range, [0.0–2.6]/100,000) and the use of breast reconstruction (median RSRR, 43.0%; interquartile range, [29.9%–62.8%]) across HSAs. Higher plastic surgeon density was positively correlated with breast reconstruction rates (correlation coefficient = 0.66, P < 0.001) and inversely related to time between mastectomy and reconstruction (correlation coefficient = −0.19, P < 0.001). Non-white and publicly insured women were least likely to undergo breast reconstruction overall. Among privately insured patients, racial disparities were noted in high surgeon density areas (white = 79.0% vs. non-white = 63.3%; P < 0.001) but not in low surgeon density areas (34.4% vs 36.5%; P = 0.70). Conclusions The lack of geographic access to a plastic surgeon serves as a barrier to breast reconstruction and may compound disparities in care associated with race and insurance status. Future efforts to improve equitable access should consider strategies to ensure access to appropriate clinical expertise.
Reoperative thyroid surgery.

BACKGROUND Patients with thyroid cancer are sometimes denied repeat thyroid operations for fear of an increased risk of complications. METHODS We therefore reviewed our experience in 114 patients with benign or malignant thyroid tumors who underwent 116 thyroid reoperations with or without other procedures. All patients had undergone at least one prior thyroid operation and 16 patients had undergone from two to four thyroid operations before referral. The initial histologic diagnosis before reoperation was thyroid carcinoma in 79 patients, papillary carcinoma in 47 patients, follicular carcinoma in 17 patients, medullary carcinoma in 9 patients, and Hürthle cell carcinoma in 6 patients. Benign disease was present in 35 patients. In 62 patients with cancer, reoperations were performed because of suspected persistent or recurrent disease; one of these patients underwent two reoperations by us. In 17 patients reoperation was to complete total thyroidectomy, primarily so that radioactive iodine could be used to scan for and treat metastatic disease. RESULTS Among the 116 reoperations, 102 were completion total thyroidectomy, 8 were near-total or subtotal thyroidectomy, and 6 were completion lobectomy. Histologic examination at reoperation revealed thyroid carcinoma in 51 cases (64%) among the 79 patients who had undergone 80 operations for previous thyroid cancer. Recurrent or persistent cancer was present in 49 of 63 (78%) reoperations for patients with papillary, medullary, and Hürthle cell cancer but in only 2 of 17 (12%) patients with follicular cancer. Cancer also occurred in 8 cases (22%) of the 36 reoperations in 35 patients who initially had benign lesions. Complications included one permanent and one transient palsy of the recurrent laryngeal nerve; both occurred on the side of a previous partial or subtotal lobectomy. Other complications included temporary hypoparathyroidism in four patients, seromas in two patients, and a keloid in one patient. CONCLUSIONS This study documents that reoperations can be performed with minimal morbidity. Thus patients should not be denied the chance to undergo removal of a persistent tumor or the remnant normal thyroid tissue because of the fear of complications.
Increased Urinary Excretion of &agr;1-Microglobulin at 6 Months after Transplantation is Associated with Urinary Excretion of Transforming Growth Factor-&bgr;1 and Indicates Poor Long-Term Renal Outcome

Background. Albumin and &agr;1-microglobulin (&agr;1M) are absorbed by two specific receptors in tubular epithelial cells. Any cell injury will disturb the reabsorption of these proteins, The increased urinary excretions of albumin or &agr;1M could thus serve as a marker of subclinical graft lesions and as an early indicator of chronic allograft dysfunction. Methods. We measured 24-hour urinary excretions of albumin, &agr;1M, and transforming growth factor (TGF)-&bgr;1 at 6 months after transplantation in 79 renal-graft recipients and recorded the changes in 24-hour creatinine clearance an average 51 (range 14–72) posttransplant follow-up months. Results. At 6 months from transplantation, 46 of 79 (58%) patients were normoalbuminuric, 25 (32%) microalbuminuric, and 8 (10%) macroalbuminuric. In normoalbuminuric patients, urinary &agr;1M/creatinine ratio was 10 times, and TGF-&bgr;1/creatinine ratio approximately 5 times, higher than in the healthy subjects but lower than in albuminuric patients. In all patients, urinary &agr;1M correlated with urinary TGF-&bgr;1 (r=0.508, P<0.001), with albumin (r=0.220, P<0.05), and with the annual changes in 24-hour creatinine clearance (r=−0.273, P<0.05). During follow-up, renal function deteriorated in 20 of 33 (60%) patients with &agr;1M/creatinine ratio greater than 5 mg/mmol, but only in 1 of 46 (2%) patients whose ratio was less than 5 mg/mmol (P<0.01), giving the ratio 5 mg/mmol or greater a 95% sensitivity to detect patients with poor long-term outcome. Conclusions. We show proximal tubular injury, measured by increased urinary &agr;1M, to be present even in normoalbuminuric patients and to be associated with increased excretion of TGF-&bgr;1 and with the annual deterioration of glomerular filtration rate. These findings show increased &agr;1M/creatinine ratio to be an early and sensitive indicator of poor long-term outcome in renal-transplant patients
Enhancement of extracapsular polysaccharide synthesis in Klebsiella pneumoniae by RmpA2, which shows homology to NtrC and FixJ

We determined the complete nucleotide sequence of a 2.1-kb HindIII-EcoRI fragment that was cloned from a resident large plasmid of Klebsiella pneumoniae Chedid, a highly virulent and mucoviscous strain of the O1:K2 serotype. This fragment encoded an ability to enhance K2 capsular polysaccharide synthesis in K. pneumoniae, and a 636-bp open reading frame (rmpA2) was found. The 411-bp rmpA reported to be involved in the virulence and mucoid phenotypes of K. pneumoniae by Nassif et al. (Mol. Microbiol. 3:1349-1359, 1989) was a part of rmpA2. Eighty percent homology in nucleotide sequence was found between rmpA2 and rmpA in the corresponding regions. The central domain of the deduced amino acid sequence of RmpA2 showed considerable homology to the central domains of NtrC of K. pneumoniae and Escherichia coli, to which the sigma factor of RNA polymerase binds. The C-terminal domain of RmpA2 also demonstrated considerable homology with the putative helix-turn-helix motifs of LuxR of Vibrio fischeri and FixJ of Rhizobium meliloti. Moreover, RmpA2 also showed some homology in its N- and C-terminal regions to those of RcsA, a transcriptional activator for colanic acid synthesis in E. coli. On the other hand, a sequence upstream of rmpA2 was found to be highly homologous to insertion sequence 3 of members of the family Enterobacteriaceae. Southern hybridization analysis suggested that rmpA2 exists on the large plasmids of all mucoviscous virulent K2 strains but not on those of the slightly mucoviscous avirulent strains. Freeze substitution electron microscopy and fluorescent-antibody staining with anti-K2 serum revealed that K. pneumoniae Chedid has a dense and thick capsule (180 nm) with dense extracapsular substance, whereas K. pneumoniae K2-215, one of the slightly mucoviscous and avirulent strains, has a capsule which is looser and thinner (120 nm) than that of strain Chedid and no extracapsular substance. Introduction of rmpA2 into K2-215 as well as reference strains K. pneumoniae K9 and K72 resulted in a change of the colony phenotype to highly mucoviscous through abundant production of extracapsular substance which reacted with anti-K2, -K9, or -K72, respectively, as did their parental strains. From these results, it is suggested that RmpA2 belongs to the family of transcriptional regulators and confers a highly mucoviscous phenotype on cells of various serotypes of K. pneumoniae by enhancing extracapsular polysaccharide synthesis.
Trends in Assortative Mating and Offspring Outcomes

We study assortative mating of Norwegian parents over five decades and its consequences for offspring outcomes. Parents are characterized by the earnings decile of their parents (the offspring's grandparents) as an indicator of social class. While assortative mating has remained stable across decades, parenthood has become more skewed toward the higher classes. Examining the influence on offspring education and employment, we find that the marginal effect of one parent's class is smaller the higher is the class of the other. Overall, mating trends have contributed to slight improvements in average education and employment and reduced inequality in the offspring generation.