Miracle-dz/UniFAR-FTUs huggingface dataset:
Quantifying Pseudomonas aeruginosa Adhesion to Contact Lenses1

Purpose. Pseudomonal keratitis can occur in soft contact lens wearers following compromise of the corneal epithelium and exposure to pathogens. This study was initiated to determine if Pseudomonas aeruginosa has the ability to adhere preferentially to unused contact lenses made from different FDA group polymers. Methods. Pseudomonas aeruginosa (strain PA01) was grown to its early stationary phase and diluted into phosphate-buffered saline to a concentration of 12,000 to 16,000 cells/mL. Samples from each of the four FDA-designated polymer classes used for the manufacture of soft lenses were incubated in pseudomonal inoculant for 4 hours. The lenses were removed and the number of bacteria bound was quantified using electrical impedance particle counting. Results. A lens constructed from a group I polymer (nonionic polymer with <50% water) bound the fewest bacteria (7.2% of total cells ± 1.0 SD) whereas a lens made from group II polymers (nonionic polymer with >50% water) showed the highest level of bacterial binding (42% of total cells ± 4.5 SD). Lenses constructed from group III and IV polymers showed intermediate levels of bacterial binding (28.4% ± 1.8 SD and 29.3% ± 1.7 SD, respectively). Conclusions. The polymer type used to construct the contact lens may influence subsequent bacterial adhesion events. Contact lenses made from nonionic polymers with high water content may carry higher risks of bacterial contamination.
Prevalence of homozygosity for the deleted alleles of glutathione S‐transferase mu (GSTMl) and theta (GSTTl) among distinct ethnic groutx from Brazil: relevance to enviromental carcinogenesis?

Arruda VR, Grignolli CE, Goncalves MS, Soares MC, Menezes R, Saad STO, Costa FF. Prevalence of homozygosity for the deleted alleles of glutathione S‐transferase mu (GSTMl) and theta (GSTTl) among distinct ethnic groups from Brazil: relevance to enviromental carcinogenesis? Clin Genet 1998: 54: 210–214. 0 Munksgaard, 1998
Molecular cytogenetic characterization of parental genomes in the partial amphidiploid Triticum aestivum × Thinopyrum ponticum

The wheat line PF 839197 and six hybrid derivatives from a cross between PF 839197 and Thinopyrum ponticum were cytologically characterized by fluorescent in situ hybridization (FISH). Probes for the 5S and 45S rDNA genes (pTa794 and pTa71, respectively), a highly repetitive rye sequence (pSc119.2), the synthetic oligonucleotide (AAG)5, and total genomic DNA from Th. ponticum and rye were used. In the wheat line, a 1RS.1BL translocation was revealed by the labeling patterns produced with pSc119.2 and (AAG)5, and confirmed by genomic in situ hybridization (GISH) using rye genomic DNA as a probe. Analyses of partial amphiploids confirmed previous results indicating mitotic instability, with a tendency to stabilize at 2n = 42 or 56. GISH with Th. ponticum genomic DNA showed that in one hybrid derivative, with lower chromosome numbers (2n = 42-45), chromosomes were not labeled, whereas in the hybrids with 2n = 48-56 up to 14 chromosomes were labeled. These data suggest that the original chromosome set of these hybrids was 2n = 56, and that chromosomes from both genomes were lost by mitotic instability. FISH using the rDNA probes and GISH with Thinopyrum genomic DNA suggested that cells with 2n = 56 contained an entire wheat genome plus two monoploid chromosome sets of Th. ponticum.
Applying bone-adaptive remodelling theory to ligamentous spine. Preliminary results of partial nucleotomy and stabilization

Remodelling theory is applied to show that a vertebral body of a spinal motion segment is an optimum structure. Then, the study is extended to analyze the changes in the shape of the vertebral bodies due to an "injury" to the disc and then the stabilization of the injured motion segment using a rigid fixation device. The salient finding of this study is the demonstration that it is feasible to quantify changes in the spinal structures following surgery. However, keeping in mind the complex nature of the spinal structures, the limitations inherent in this study, and the assumption made for the bone adaptive remodelling theory, one needs to very systematically increase the complexity of the spinal model, and also initiate in parallel experimental studies delineating the remodelling aspects of the spinal structures. <<ETX>>
Chebyshev-Vandermonde systems

A Chebyshev-Vandermonde matrix is obtained by replacing the monomial entries of a Vandermonde matrix by Chebyshev polynomials /> for an ellipse. The ellipse is also allowed to be a disk or an interval. We present a progressive scheme for allocating distinct nodes zk on the boundary of the ellipse such that the Chebyshev-Vandermonde matrices obtained are reasonably well-conditioned. Fast progressive algorithms for the solution of the Chebyshev-Vandermonde systems are described. These algorithms are closely related to methods recently presented by Higham. We show that the node allocation is such that the solution computed by the progressive algorithms is fairly insensitive to perturbations in the right-hand side vector. Computed examples illustrate the numerical behavior of the schemes. Our analysis can also be used to bound the condition number of the polynomial interpolation operator defined by Newton's interpolation formula. This extends earlier results of Fischer and the first author.
Localized limb cutaneous metastases

Cutaneous metastatic disease may be evident in a variety of forms and locations. Anatomically, it may on occasion be confined as localized limb metastases. We report on two patients with localized limb metastases, one from melanoma and the other from Merkel cell carcinoma. Patients with localized limb metastasis have a poor prognosis; however, treatment options not available for generalized cutaneous metastatic disease, such as amputation or isolated limb perfusion with chemotherapeutic agents, can be at times be beneficially employed. J. Surg. Oncol. 1998;67:261–264. © 1998 Wiley‐Liss, Inc.
Myelination by Schwann cells in the absence of extracellular matrix assembly

Assembly of extracellular collagen fibrils and Schwann cell basal lamina has previously been identified as a prerequisite for compact myelin formation in the peripheral nervous system. Synthesis of this extracellular matrix (ECM) in vitro required the presence of serum and ascorbic acid. Using rat embryonic dorsal root ganglion neurons and Schwann cells, we have developed a fully defined medium in which myelination occurs. In the absence of ascorbic acid, normal myelin was formed without ECM assembly. This demonstrates that although myelination and ECM assembly are usually closely linked, ECM formation is not a prerequisite for myelination in vitro. GLIA 23:383–388, 1998. © 1998 Wiley‐Liss, Inc.
Towards the Reduction of Medication Errors in Orthopedics and Spinal Surgery: Outcomes Using a Pharmacist-Led Approach

Study Design. Evaluation of medication ordering errors discovered on an orthopedic/spinal in-patient hospital unit and efforts initiated to reduce them. Objective. In this study the authors aimed to assess the frequency of medication ordering errors and to examine the impact of local measures set forth to reduce their occurrence. Summary of Background Data. Since the release of the 2000 Institute of Health report: “To Err is Human”; in-hospital medical errors have been recognized as being unacceptably high; the consequence being preventable death rates estimated near 125,000 patients per year. The most common of errors are those involving medications. Methods. The study consisted of 2 parts. In part 1, the charts from 82 consecutive patients admitted to the Orthopedic/Spine Surgical Unit were assessed to determine the frequency, type, and potential severity of medication ordering errors. Several programs to reduce such errors were subsequently instituted and included: improved chart surveillance by pharmacists, a newly developed medication/history form given to and reviewed with patients before surgery, in-service education of preoperative nursing staff, patient database form changes, and requests for patients to bring their medications on admission. Part 2, including 87 patients, assessed the impact of these measures. Results. In part 1, medication errors were detected in 62% of orders overall. Of these, 43% were found to be of moderate or high potential for harm. After the institution of the above measures (part 2), overall errors were reduced by 31%; moderate/high risk potential harm was reduced by 64%; and errors of omission were detected twice as often. Conclusion. Medication errors in ordering are common in orthopedics. We found in part 1 that a chart review and patient interview by the pharmacy team can detect and correct these before reaching the patient. Furthermore, we found in part 2 that the risk could be further reduced by the implementation of pharmacist-led: patient education, education of preoperative nursing personnel, improvement of forms used for data collection, and having the patients bring all of their medications on admission.
Bandwidth Limitations for Scattering of Higher Order Electromagnetic Spherical Waves With Implications for the Antenna Scattering Matrix

Various physical limitations in electromagnetic theory and antenna theory have received considerable attention recently. However, there are no previous limitations on the scattering of higher order electromagnetic vector spherical waves, despite the widespread use of spherical wave decompositions. In the present paper, bandwidth limitations on the scattering matrix are derived for a wide class of heterogeneous objects, in terms of their electrical size, shape and static material properties. In particular, it is seen that the order of the dominating term in the Rayleigh limit increases with the order of the spherical wave. Furthermore, it is shown how the limitations place bounds on the antenna scattering matrix, thus introducing a new approach to physical limitations on antennas. Comparisons to other types of antenna limitations are given, and numerical simulations for two folded spherical helix antennas and a directive Yagi-Uda antenna are included to illuminate and validate the theory. The results in this paper are derived using a general approach to derive limitations for passive systems: First, the low-frequency asymptotic expansion of the scattering matrix of a general scatterer is derived. This gives a set of sum rules, from which the limitations follow.
Safety and Immunogenicity of Sequential Rotavirus Vaccine Schedules

BACKGROUND AND OBJECTIVES: Although both licensed rotavirus vaccines are safe and effective, it is often not possible to complete the schedule by using the same vaccine formulation. The goal of this study was to investigate the noninferiority of the immune responses to the 2 licensed rotavirus vaccines when administered as a mixed schedule compared with administering a single vaccine formulation alone. METHODS: Randomized, multicenter, open-label study. Healthy infants (6–14 weeks of age) were randomized to receive rotavirus vaccines in 1 of 5 different schedules (2 using a single vaccine for all doses, and 3 using mixed schedules). The group receiving only the monovalent rotavirus vaccine received 2 doses of vaccine and the other 4 groups received 3 doses of vaccine. Serum for immunogenicity testing was obtained 1 month after the last vaccine dose and the proportion of seropositive children (rotavirus immunoglobulin A ≥20 U/mL) were compared in all the vaccine groups. RESULTS: Between March 2011 and September 2013, 1393 children were enrolled and randomized. Immune responses to all the sequential mixed vaccine schedules were shown to be noninferior when compared with the 2 single vaccine reference groups. The proportion of children seropositive to at least 1 vaccine antigen at 1 month after vaccination ranged from 77% to 96%, and was not significantly different among all the study groups. All schedules were well tolerated. CONCLUSIONS: Mixed schedules are safe and induced comparable immune responses when compared with the licensed rotavirus vaccines given alone.
Clinico-pathological profile of sinonasal masses: An experience in tertiary care hospital of Uttarakhand

Background: The purpose of this study was to classify various types of non-neoplastic and neoplastic lesions presenting as sinonasal mass and characterize their clinico-pathological profile in a tertiary care center in the state of Uttarakhand. Materials and Methods: This was a prospective study where 110 cases of sinonasal masses were included over a period of 12 months. Clinico-pathological study was carried out in these cases. A provisional diagnosis was made after clinical assessment and radiologic investigations, but final diagnosis was made after histopathologic examination. Observations: The number of non-neoplastic lesions were more than the neoplastic lesion, 60% versus 40% respectively. In the neoplastic group, 19.8% and 23.76% patients presented with benign and malignant lesion, respectively. The incidence was more predominant in the age group of 11-20 years (22.72%) with male to female ratio of 1.08:1. In our study, among non-neoplastic lesions the occurrence of sinonasal polyps was highest seen in 80.30% cases. In neoplastic lesions, angiofibroma was most common benign lesion seen in 35% cases. Carcinoma nasal cavity was the commonest malignant lesion seen in 45.83% cases. In 3.63% patients, clinical and radiologic diagnosis was not correlated with histopathologic diagnosis. Only two cases required immuno-histocytochemistry to confirm the final diagnosis. Conclusion: We concluded that for proper evaluation of a sinonasal mass, clinical, radiologic, and histopathologic evaluation should be carried out conjointly in all the cases. Histopathology always gives a confirmatory diagnosis but in few cases immuno-histocytochemistry becomes the ultimate diagnostic technique for correct and timely intervention.
On the site of impulse initiation in a neurone.

In the preceding paper (Moore & Westerfield, 1983) the effects of changes in membrane properties and non‐uniform geometry on impulse propagation and threshold parameters were investigated. In this paper the contributions of these and other parameters to the site of initiation of an impulse were determined by computer simulations using the Hodgkin‐Huxley membrane description, the cable equations, and geometry appropriate for a simplified motoneurone with a non‐myelinated axon. Antidromic invasion of action potentials into the soma was found to depend upon (a) the ionic channel rate constants (determined by the temperature), (b) the abruptness of the transition from the small‐diameter axon to the larger diameter (and increased load) of the soma‐dendrite, (c) extensions of active properties into the dendrite, and (d) density of ion channels. The location of the apparent site of initiation of impulses was not necessarily at the site of synaptic input nor the nearest active membrane. Its position depended upon (a) the fraction of the dendritic tree with excitable membrane, and secondarily on (b) the stimulus strength. Even with uniform excitability in the active membrane, the apparent site of initiation could be moved a considerable distance from the soma and the site of stimulation by appropriate choice of the various parameters noted above.
THE EFFECT OF EARLY POST CESAREAN FEEDING ON WOMEN’S SATISFACTION

Objective: Satisfaction and postoperative care of women who undergo cesarean section is an important aspect and demand due attention. Hunger and thirst are frequently encountered after cesarean delivery. These increase distressing symptom and unsatisfaction. The aim of this paper was to detect the effect of early post cesarean feeding on mothers' satisfaction. Materials and methods: This study was a controlled trial. The research population was women delivering by cesarean section in Ommolbanin hospital, Mashhad, Iran. The samples consist of 82 women randomly assigned in two groups of study and control. The study group started oral fluids 4 hours after surgery, followed by regular diet after return of bowel sounds. The control group started oral fluids 12 hours after surgery and solid food was allowed after defecation. The groups were compared about satisfaction rate before discharge with VAS scale. Data were analyzed using chi-square, Fisher exact test, Kruskal walis test, t test and Mann Whitney. Results: The mean of women's satisfaction was 96.2 in early feed group and 56.7 in the control group. There was a statistically significant difference between two groups (p=0.000). Conclusion: this study showed that early feeding increases women's satisfaction. It is a safe, easy and enforceable intervention that can be usual in surgical units.
Similar sequence specificity of mitoxantrone and VM-26 stimulation of in vitro DNA cleavage by mammalian DNA topoisomerase II.

The molecular mechanism of topoisomerase II trapping by antitumor drugs probably involves the formation of a ternary complex DNA-drug-topoisomerase II. Recent studies support the view that a drug molecule might be placed at the DNA cleavage site interacting with the two flanking base pairs and amino acid residues of the enzyme. In this work, the DNA sequence-dependent action of mitoxantrone on topoisomerase II DNA cleavage was investigated in SV40 DNA fragments and short oligonucleotides, in comparison to VM-26, 4-demethoxydaunorubicin, and mAMSA. Mitoxantrone and VM-26 had a much lower degree of selectivity than 4-demethoxydaunorubicin and mAMSA in stimulating DNA cleavage. DNA cleavage at sites that were always stimulated also by VM-26. In contrast, mitoxantrone and 4-demethoxydaunorubicin shared only 7% of cleavage sites, and about 70% of the 4-demethoxydaunorubicin-stimulated sites were also stimulated by VM-26. Unlike what is generally seen with anthracyclines, the structurally related drug, mitoxantrone, stimulated cleavage also at DNA sites observed without drugs. Local base preferences at the cleavage site as determined by statistical analysis showed that mitoxantrone preferentially cleaved the DNA at sites with a cytosine or a thymine at position-1. However, strong DNA cleavage stimulation by mitoxantrone was favored by specific base pairs at the next positions flanking the cleaved bond (positions -2 and +2) and at positions +8 and +9. Effects of base mutations on drug stimulation of DNA cleavage in short DNA oligonucleotides independently showed that a pyrimidine at position -1 is required for mitoxantrone action. (ABSTRACT TRUNCATED AT 250 WORDS)
Self‐Determination, Perception of Peer Pressure, and Drinking Among College Students1

Based on self-determination theory (Deci & Ryan, 1985b), the present research tested a model that incorporated motivational orientation, extrinsic reasons for drinking, and perceptions of peer pressure as predictors of drinking among college students. In a sample of undergraduates, support was found for a path model in which global motivation predicted extrinsic reasons for drinking, which predicted perceptions of peer pressure, which in turn predicted alcohol consumption. In addition, the relation between peer pressure and drinking was stronger for those who were oriented toward feeling controlled. Support was found for a similar model in a sample of fraternity students. Results support previous research on self-determination and health.
Vaccination with a synthetic peptide modulates lymphocytic choriomeningitis virus-mediated immunopathology

Vaccination with a nucleopeptide (NP 118; amino acids 118 to 132) representing a cytotoxic T-cell epitope of lymphocytic choriomeningitis virus (LCMV) can modulate immunopathology. Immunization with NP 118 protected H-2d mice against intracerebral infection with the LCMV-ARMSTRONG isolate. However, when NP 118-primed H-2d mice were challenged intracerebrally with an intermediate dose (5 x 10(4) PFU) of the LCMV-DOCILE strain, all mice primed with NP 118 emulsified in incomplete Freund's adjuvant died, whereas unprimed mice survived. Correspondingly, peptide vaccination enhanced specifically the cytotoxic T-cell response, influencing the critical balance between T-cell response and virus spread.
Premature discontinuation of clinical trial for reasons not related to efficacy, safety, or feasibility.

# Premature discontinuation of clinical trial for reasons not related to efficacy, safety, or feasibility {#article-title-2} Editorial by Evans and Pocock When investigators embark on a clinical trial, they naturally expect that the journey will end with the completion of the scheduled patient follow up and publication of the results. Some trials may sink en route because of organisational or ethical reasons, and such misfortunes must be accepted. Sometimes, however, trials are scuttled by their sponsors. Such premature discontinuation not only is frustrating for investigators but may have important medical implications. In this article we analyse the case of a clinical trial that was recently stopped for financial reasons, discuss the consequences of such discontinuations, and make some proposals to avoid recurrence. #### Summary points Some trials are discontinued prematurely by their sponsor for strategic reasons Clinical trials should be discontinued only for reasons pertaining to efficacy, safety, or feasibility Premature discontinuation of trials for strategic reasons deceives the patients, jeopardises the patient-doctor relationship, and harms the medical community Giving more power to steering committees that are mostly independent of the sponsor and include patient representatives, may limit the risk of premature discontinuation of trials for strategic reasons Public financial and scientific participation in some trials and increasing the length of patents may be useful Although two trials have shown the efficacy of statins for primary prevention of cardiovascular disease in middle aged people with hypercholesterolaemia, 1 2 the benefits and costs of such treatments in older men and women are unclear, especially in low cardiovascular risk populations.3-5 In April 1997, a group of French academics submitted to several pharmaceutical companies the protocol of the first placebo controlled trial of a statin for primary prevention in hypercholesterolaemic men and women aged 70 to 85 in low cardiovascular risk countries. Novartis agreed to fund the study in June 1998, when the company executives were convinced that this study was …
The use of coenzyme Q10 and DHEA during IUI and IVF cycles in patients with decreased ovarian reserve

Abstract Objective: The objective of this study is to compare the combination of dehydroepiandrosterone (DHEA) and coenzyme Q10 (CoQ10) (D + C) with DHEA alone (D) in intrauterine insemination (IUI) and in vitro fertilization (IVF) cycles among patients with decreased ovarian reserve. Methods: We retrospectively extracted data from patients charts treated by DHEA with/without CoQ10 during IUI or IVF between February 2006 and June 2014. Prestimulation parameters included age, BMI, day 3 FSH and antral follicular count (AFC). Ovarian response parameters included total gonadotropins dosage, peak serum estradiol, number of follicles > 16 mm and fertilization rate. Clinical outcomes included clinical and ongoing pregnancy rates. Results: Three hundred and thirty IUI cycles involved D + C compared with 467 cycles of D; 78 IVF cycles involved D + C and 175 D. In both IUI and IVF, AFC was higher with D + C compared with D (7.4 ± 5.7 versus 5.9 ± 4.7, 8.2 ± 6.3 versus 5.2 ± 5, respectively, p < 0.05). D + C resulted in a more follicles > 16 mm during IUI cycles (3.3 ± 2.3 versus 2.9 ± 2.2, respectively, p = 0.01), while lower mean total gonadotropin dosage was administered after D + C supplementation compared with D (3414 ± 1141 IUs versus 3877 ± 1143 IUs respectively, p = 0.032) in IVF cycles. Pregnancy and delivery rates were similar for both IUI and IVF. Conclusion: D + C significantly increases AFC and improves ovarian responsiveness during IUI and IVF without a difference in clinical outcome.