Miracle-dz/UniFAR-FTUs huggingface dataset:
Cuprizone‐induced demyelination in CNP::GFP transgenic mice

Cuprizone (bis‐cyclohexanone oxaldihydrazone) was previously shown to induce demyelination in white matter enriched brain structures. In the present study we used the cuprizone demyelination model in transgenic mice expressing the enhanced green fluorescent protein (GFP) under the 2′‐3′‐cyclic nucleotide 3′‐phosphodiesterase (CNPase) promoter. The use of these particular transgenic mice allows easy detection of cells belonging to the entire oligodendroglial (OLG) lineage, ranging from OLG precursors to mature myelinating OLGs. We were able to evaluate the precise extent of oligodendroglial cell damage and recovery within the murine adult central nervous system (CNS) after inducing demyelination by acute cuprizone intoxication. A generalized loss of GFP+ cells was observed after cuprizone exposure and correlated with a decline in myelin basic protein (MBP) expression. OLGs were depleted in many brain areas that were previously thought to be unaffected by cuprizone treatment. Thus, in addition to the well‐known cuprizone effects on the medial corpus callosum, we also found a loss of GFP+ cells in most brain structures, particularly in the caudatus putamen, cortex, anterior commissure, olfactory bulb, hippocampus, optic chiasm, brainstem, and cingulum. Loss of GFP+ cells was accompanied by extensive astrogliosis and microglial activation, although neurons were not affected. Interestingly, cuprizone‐treated animals showed both activation of GFAP expression and a higher proliferation rate in subventricular zone cells. A week after cuprizone removal from the diet, GFP+ oligodendroglial cells began repopulating the damaged structures. GFP expression precedes that of MBP and allows OLG detection before myelin restoration. J. Comp. Neurol. 518:2261–2283, 2010. © 2010 Wiley‐Liss, Inc.
Work related mortality from gastrointestinal diseases and alcohol among seafarers employed in British merchant shipping from 1939 to 2002.

OBJECTIVES To investigate work related mortality from gastrointestinal diseases and from alcohol among seafarers who were employed in British merchant shipping from 1939 to 2002. METHODS A longitudinal study, based on official mortality files from 1976-2002 and official mortality returns from 1939-1975, with a population of 7.29 million seafarer-years at risk. RESULTS From 1939 to 2002, there were 864 deaths from gastrointestinal diseases and 72 from alcoholism. Overall mortality from gastrointestinal diseases fell from 18.4 per 100,000 in 1939-49 to 9.3 in 1970-79 and 0.3 in 1990-2002. Mortality from alcoholism, and from alcohol-related diseases such as liver cirrhosis and diseases of the pancreas, increased up to the 1960s or 1970s, but fell thereafter. From 1950 to 1972, mortality from gastrointestinal diseases was 1.8 times higher among Asian seafarers compared to British seafarers, largely because of liver disease, peritonitis and peptic ulcer. At the time of the last censuses of seamen in 1961 and 1971, compared with the general British male working aged population, morality among British seafarers was greatly increased for peritonitis and alcoholism but not for most other gastrointestinal diseases. CONCLUSIONS Sharp reductions in mortality from gastrointestinal diseases and from alcoholism since the 1970s contrasts with increases among the general British population, and are largely because of the "flagging-out" of most British deep sea ships, and consequent reductions in long voyages, as well as reductions in alcohol consumption among seafarers at work. Largely because of the healthy worker effect, seafarers were usually only at increased risks from particularly acute diseases.
Anatomic and Electrophysiologic Evidence for a Proconvulsive Circuit in the Dentate Gyrus of Reeler Mutant Mice, an Animal Model of Diffuse Cortical Malformation

Although cortical malformations (CMs) are often associated with epilepsy, the underlying mechanisms are unknown. The reeler mouse is a model of CM with enhanced susceptibility to epileptiform activity, including the in vitro dentate gyrus, a region normally resistant to seizures. In this study, field potential recordings in hippocampal slices and the Timm stain were used to examine mossy fiber distribution in the dentate gyrus. In artificial cerebrospinal fluid containing bicuculline, 100% of reeler slices and 0% of control slices had spontaneous and antidromic evoked prolonged negative field potential shifts that were blocked by glutamate receptor antagonists. Sections from reeler mice, but not controls, exhibited a dark band of Timm’s stain at the molecular layer/granule cell layer border. These data reveal that mossy fiber distribution is altered in reeler mice and coincides with the presence of an abnormal proconvulsive glutamatergic circuit.
Temporal Profile of Stem Cell Division, Migration, and Differentiation from Subventricular Zone to Olfactory Bulb after Transient Forebrain Ischemia in Gerbils

The stage of neurogenesis can be divided into three steps: proliferation, migration, and differentiation. To elucidate their detailed relations after ischemia, the three steps were comprehensively evaluated, in the subventricular zone (SVZ) through the rostral migratory stream (RMS) to the olfactory bulb (OB), in adult gerbil brain after 5 minutes of transient forebrain ischemia. Bromodeoxyuridine (BrdU), highly polysialylated neural cell adhesion molecule (PSA-NCAM), neuronal nuclear antigen (NeuN), and glial fibrillary acidic protein (GFAP) were used as markers for proliferation, migration, and differentiation, respectively. The number of BrdU-labeled cells that coexpressed PSA-NCAM and the size of PSA-NCAM–positive cell colony increased in the SVZ with a peak at 10 d after transient ischemia. In the RMS, the number of BrdU-labeled cells that coexpressed PSA-NCAM increased, with a delayed peak at 30 d, when the size of RMS itself became larger and the number of surrounding GFAP-positive cells increased. In the OB, BrdU + NeuN double positive cells were detected at 30 and 60 d. NeuN staining and terminal deoxynucleotidyl dUTP nick-end labeling staining showed no neuronal cell loss around the SVZ, and in the RMS and the OB after transient ischemia. These findings indicate that transient forebrain ischemia enhances neural stem cell proliferation in the SVZ without evident neuronal cell loss, and has potential neuronal precursor migration with activation of GFAP-positive cells through the RMS to the OB.
Proteomic analysis on a high salt tolerance introgression strain of Triticum aestivum/Thinopyrum ponticum

Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt‐tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV‐irradiated Agropyron elongatum (Thinopyrum ponticum Podp). We report here a comparative proteomic analysis to investigate variety‐specific and salt‐responsive proteins between seedling‐roots of Shanrong No. 3 and Jinan 177. In total, 114 spots reproducibly presented differential expression patterns on 2‐DE maps. Of them, 34 were variety‐specific and 49 were salt‐responsive. We identified 110 spots by MALDI‐TOF MS and partially confirmed by MALDI‐TOF‐TOF MS, and functionally classified them into signal transduction, transcription and translation, transporting, chaperones, proteolysis and detoxification, etc. Meanwhile, we also found the alteration of protein expression of Shanrong No. 3 through inhibition of old proteins and production of novel ones, change in abundance and sensitivity of some nonsalt‐responsive and salt‐responsive proteins, as well as PTMs. Furthermore, comparison between proteome and transcripteome using cDNA microarray showed that there were only 20 proteins with abundances correlative to signal densities of corresponding EST probes. This study gives us a global insight into proteomic difference between Shanrong No. 3 and Jinan 177 in constitute and to salt‐response.
Fiscal Multipliers and Monetary Policy: Reconciling Theory and Evidence

Fiscal multipliers are typically observed to be moderate, which should, according to standard macroeconomic theory, be associated with real interest rates increasing with government spending. However, monetary policy rates have been found to decrease, which should – in theory – lead to large multipliers. In this paper, we rationalize these puzzling observations by accounting for responses of interest rates that are more relevant for private sector transactions than the monetary policy rate. We provide evidence that real interest rates on relatively illiquid assets and interest rate spreads which measure liquidity premia tend to increase after a government spending hike. We show that an otherwise standard macro model can explain diverging interest rate responses and moderate fiscal multipliers consistent with the data by accounting for an interest rate spread that decreases with the relative demand for less liquid assets. Our analysis indicates that neither a policy rate reduction nor a fixation at the zero lower bound are sufficient to induce large fiscal multipliers.
Demographic Challenges for the Future Business Leader: Evidence from a Greek Survey

Purpose The purpose of this paper is to identify the challenges posed to business leaders from major global demographic changes expected in the workforce composition, such as higher participation of women and more active involvement of elder and more experienced people in the future workforce. Design/methodology/approach The authors ran a survey among 733 middle managers, focusing on their ideal leadership behavior. The Global Leadership and Organizational Behavior project methodology was applied for data collection and analyses. Findings Several significant differences were established in leadership ideals according to gender, age and managerial experience. The differences based on gender had higher practical significance than the ones based on age and experience of the respondents. Research limitations/implications The paper adds up to the pertinent in the implicit leadership approach dialogue over the existence of stable, global and unchanging aspects of leadership. Practical implications Leader behaviors traditionally deemed as positive, such as the participative and charismatic/value-based leadership, are shown to be critical for the future business leader. Leadership development in organizations should focus on these two aspects. Social implications Leaders who can meet the expectations of diverse groups of people will be able to achieve inclusion of least privileged groups at the business level, leading to higher inclusion at the social, aggregate level. Originality/value The paper is the first to explore how documented demographic trends could affect the way that leadership will evolve in the near future.
Rule extraction using Support Vector Machine based hybrid classifier

Support Vector Machines (SVMs) have become an increasingly popular tool for machine learning tasks involving classification and regression, and have shown superior performance compared to other machine learning techniques. In this paper we propose a hybrid classification technique to extract fuzzy rules from the support vector machine and evaluate the rules against decision tree classifier constructed from the same support vector machine. The hybrid approach proceeds in three major steps. In the first step we use training patterns with class labels to build an SVM model that gives the support vectors with acceptable accuracy. Fuzzy rules are generated using the extracted support vectors during second step. In the final step the resulting rule set is tested using the test data of the problem. The quality of the extracted rules is then evaluated in terms of accuracy and fidelity. It is found that the proposed hybrid approach using fuzzy rules yielded highest accuracy and fidelity compared to hybrid with decision tree classifier.
Does it Pay to Communicate with Firms? Evidence from Firm Site Visits of Mutual Funds

By using a unique dataset on mutual fund visits to listed firms in China, we investigate whether mutual funds are able to obtain private information or benefit from their communication with firms. Our findings are as follows: 1) such communication significantly increases the subsequent trading magnitude of mutual funds; 2) mutual fund trades that rely on communication significantly predict the unexpected earnings of visited firms, which is further supported by IV-regressions that use the number of direct flights between two cities as the instrument of communication; and 3) comprehensiveness of communication topics and firms’ information environment significantly affect the benefits that mutual funds obtain from communication. Our results are robust to alternative measures and specifications, and provide insights for regulators who are concerned with fair disclosure.
Comparative localization of epileptic foci in partial epilepsy by PCT and EEG.

One or more interictal positron computed tomograms of 18F-fluorodeoxyglucose were obtained on 50 patients with partial seizure disorders. Ictal as well as interictal electroencephalographic (EEG) data were available for all 50 patients, with scalp, sphenoidal, and depth electrode recordings done on 27 and scalp and sphenoidal recordings alone on 23. Thirty-five patients demonstrated one or more abnormal interictal zones of hypometabolism, while combined EEG studies were localizing for 36. There were considerable disagreements between the location of metabolic deficits and the epileptic focus revealed by individual scalp and depth EEG recorded ictal and interictal epileptiform activity; however, there was good correlation between the site of focal hypometabolism and the epileptic focus determined by the combined results of all electrophysiological studies. When focal hypometabolism and focal nonepileptiform EEG abnormalities (i.e., slow waves and attenuation of fast rhythms) were both present in the same patient, their localization agreed completely. Metabolic and combined electrophysiological techniques both occasionally produced false positive as well as false negative results. When used together, the EEG can confirm that a hypometabolic zone is epileptogenic, while FDG scans may indicate whether an epileptic EEG focus represents a lesion or propagation from a distant site.
Journals Should Publish All “Null” Results and Should Sparingly Publish “Positive” Results

To the Editor: The editorial by Rebbeck et al. ([1][1]) is timely and important. Here, I share some thoughts on this debate. The X team (real but anonymous here) meets successfully most proposed criteria. X has published nine articles on mostly brand new (but also some replicated) gene-disease
Incidence of and risk factors for hospitalizations in systemic lupus erythematosus: a prospective study of the Hopkins Lupus Cohort.

OBJECTIVE To determine the incidence of and risk factors for hospitalization in systemic lupus erythematosus (SLE). METHOD A prospective study of hospitalizations in 1989 and 1990 in the Hopkins Lupus Cohort was conducted. RESULTS Of 261 patients with SLE in the Hopkins Cohort, 147 (56.3%) were hospitalized in 1989 or 1990. The incidence of hospitalization was 0.69 admissions/person-year. The mean length of stay was 9.6 days +/- 12.7 (SD). Activity of SLE accounted for 35% of admissions. Risk factors for hospitalization for active lupus in 1990 included activity of SLE in 1989 (measured by either the highest physician's global assessment in 1989, p = 0.004, highest lupus activity index (LAI) score in 1989, p = 0.002, or highest systemic lupus erythematosus disease activity index score in 1989, p = 0.008), an average prednisone dose greater than 10 mg in 1989 (p = 0.002), and the presence of neurologic lupus in 1989 (p = 0.02). Medical admissions other than for active SLE accounted for an additional 27% of admissions; one-half of these admissions (47%) were for complications of SLE and/or its treatment, with coronary artery disease the most common (21% of medical admissions). Infections were responsible for 14% of admissions. The majority of infections were bacterial (90%). Risk factors for hospitalization for infection in 1990 included active SLE in 1989 (p = 0.03), average prednisone dose of greater than 10 mg in 1989 (p = 0.04), immunosuppressive drug use in 1989 (p = 0.003), neurologic SLE in 1989 (p = 0.02) and previous hospitalization in 1989 (p = 0.03). CONCLUSION Hospitalization is common in the Hopkins Lupus Cohort, with active SLE (35%), infection (14%, infection and/or active SLE; 11%, infection alone) and medical complications of SLE (13%) as the 3 most common causes.
Stabilizing an Unstable Complex Economy

This paper analyzes a range of alternative specifications of the interest rate policy rule within a macroeconomic, stock-flow consistent, agent-based model. In this model, firms' leverage strategies evolve under the selection pressure of market competition. The resulting process of collective adaptation generates endogenous booms and busts along credit cycles. As feedback loops on aggregate demand affect the goods and the labor markets, the real and the financial sides of the economy are closely interconnected. The baseline scenario is able to qualitatively reproduce a wide range of stylized facts, and to match quantitative orders of magnitude of the main economic indicators. We find that, despite the implementation of credit and balance sheet related prudential policies, the emerging dynamics feature strong instability. Targeting movements in the net worth of firms help dampen the credit cycles, and simultaneously reduce financial and macroeconomic volatility, but does not eliminate the occurrence of financial crises along with high costs in terms of unemployment.
Collective action and labour militancy interrupted: Back-to-work legislation and the state of permanent exceptionalism at Air Canada

Using data collected from survey responses and interviews conducted in 2014, this study examines the consequences of back-to-work legislation from the perspective of customer service workers at Air Canada represented by Unifor Local 2002. By examining union attitudes, opinions of strikes, wildcat actions and back-to-work legislation deployed in 2011 and 2012, the study concludes that this type of legislation functioned to protect the interests of the employer in an ongoing process of corporate restructuring. Such ad hoc legislative measures, defined by political economists as ‘permanent exceptionalism’, further undermines the industrial pluralist regime that is the foundation of Canadian labour relations.
Mdm2 antagonists induce apoptosis and synergize with cisplatin overcoming chemoresistance in TP53 wild‐type ovarian cancer cells

Ovarian cancer (OVCa) is the leading cause of death from gynecological malignancies. Although treatment for advanced OVCa has improved with the introduction of taxane–platinum chemotherapy, the majority of patients will develop resistance to the treatment, leading to poor prognosis. One of the causes of chemoresistance is the reduced ability to undergo apoptosis. Cisplatin is a genotoxic drug that leads cells to apoptosis through the activation of the p53 pathway. Defective signaling in this pathway compromises p53 function, and thus cisplatin does not induce apoptosis. A new group of nongenotoxic small molecules called Nutlins have been developed to inhibit p53‐Mdm2 binding, inducing apoptosis in chemoresistant tumors through the activation of the p53 pathway. The wild‐type p53 cisplatin‐resistant ovarian cancer cell‐line A2780cis was used to test the effect of Nutlin‐3a (Nut3a) on apoptosis response. The results showed that Nut3a synergized with cisplatin, inducing cell‐cycle arrest in G2/M and potentiating apoptotic cell death. Increased apoptosis was also induced in wild‐type TP53 primary OVCa cultures by double cisplatin–Nut3a treatment. In conclusion, Nut3a appears to sensitize chemoresistant OVCa cells to cisplatin, inducing apoptosis. As increased response was generalized in primary tumors, this cisplatin–Nut3a combination could be useful for the treatment of patients harboring wild‐type TP53 who do not respond to standard chemotherapy.
Pulmonary , gastrointestinal and urogenital pharmacology E ff ect of cardamonin on hepatic ischemia reperfusion induced in rats : Role of nitric oxide

Ischemia reperfusion (I/R) injury is a cellular damage in a hypoxic organ following the restoration of oxygen delivery. It may occur during organ transplantation, trauma and hepatectomies. Nitric oxide (NO) effects during hepatic I/R are complicated. The iNOS-derived NO has a deleterious effect, whereas eNOS-derived NO has a protective effect in liver I/R. Cardamonin (CDN) is an anti-inflammatory molecule and a novel iNOS inhibitor, and Nω-Nitro-L-arginine (L-NNA) is a NOS inhibitor. L-Arginine is a precursor of NOS. This study was designed to investigate the possible protective effects of CDN on hepatic I/R and the role of NO. Wistar rats were randomly divided into 5 groups (Sham, I/R, CDN, L-NNA and L-arginine). Liver ischemia was induced for 45 min then reperfusion was allowed for 1 h. L-Arginine and CDN ameliorated the deleterious effects of I/R through reducing the oxidative stress and hepatocyte degeneration. Both molecules decreased the elevated inflammatory cytokines and increased the antiapoptotic marker, Bcl2. Both agents increased NO and eNOS expression and decreased iNOS expression. In conclusion, increased NO/eNOS and suppression of iNOS expression have protective effects on I/R injury. While inhibition of eNOS and reduction of NO have deleterious effects on I/R injury. For the first time, we demonstrated that cardamonin improved functional and structural abnormalities of the liver following I/R by improving oxidative stress and inflammation and increasing the availability of NO produced by eNOS. Treatment with cardamonin could be a promising strategy in patients with hepatic I/R injury in different clinical
Palatal anesthesia: comparison of four techniques for decreasing injection discomfort.

INTRODUCTION The aim of this study was to evaluate the patient's perceived pain response to injection and anesthetic deposition for the greater palatine nerve block. METHODS Heft-Parker Visual Analog Scale (VAS) pain scale measurements were used to compare the following techniques for the injection: (1) control (no concurrent stimulation), (2) pressure, (3) pressure and topical anesthetic (20% benzocaine), and (4) pressure and cold (TFE). Forty-two volunteers, 21 male and 21 female, participated in the study. A bilateral model was used on each patient to give an injection on each side of the palate with two different techniques followed by the next appointment (> or = two weeks later), when the two other injection techniques were used. Following injection given in the supine position, the patients were returned to an upright position and asked to rate their pain on a VAS. RESULTS Pain upon needle insertion appears less than that of anesthetic deposition. There was no statistically significant difference in perceived pain response among the four techniques, the visit, the order, the side, or patient gender at either time point. Following the application of Endo Ice, 81% of participants reported a sore on their palate occurring two to 48 hours after cold application and persisting for one to 10 days. The pain score for this injection had a mean value of 30% (51.4/170). CONCLUSIONS This prospective, single-blind study evaluating three injection techniques to reduce posterior palatal injection pain to a control injection method showed no significant reduction in pain with any of the three techniques. Furthermore, 1,1,1,2-tetrafluoroethane placed with pressure for 10 seconds appeared injurious to the oral mucosa.