Miracle-dz/UniFAR-FTUs huggingface dataset:
Improved Serological Diagnosis Stresses the Major Role of Campylobacter jejuni in Triggering Guillain-Barré Syndrome

ABSTRACT Guillain-Barré syndrome (GBS) is a postinfectious autoimmune polyradiculoneuropathy. The most frequent antecedent pathogen is Campylobacter jejuni, followed by cytomegalovirus. However, more than 40% of GBS cases currently cannot be attributed to triggering events. This might be due to the shortcomings of the serological assays used for diagnosing infections, in particular for C. jejuni. In our study investigating 36 patients with acute GBS, standard serological methods identified the triggering viral or bacterial etiology in only 25% of cases. However, using a highly specific enzyme-linked immunosorbent assay based on two recombinant outer antigens encoded by C. jejuni genes Cj0017 (P39) and Cj0113 (P18), we found serological evidence of a preceding C. jejuni infection in 80.6% of the patients but in only 3.5% of the controls. We conclude that the role of C. jejuni in triggering GBS has been greatly underestimated.
Effects of three nickel salts on germinating seeds of Grevillea exul var. rubiginosa, an endemic serpentine Proteaceae.

BACKGROUND AND AIMS Serpentine soils are usually quite infertile, arid and toxic, mainly because they contain high levels of heavy metals such as Ni. The aim of the present work was to assess the effects of Ni on the germinating seeds of Grevillea exul var. rubiginosa, an endemic serpentine Proteaceae of New Caledonia. In addition, the distribution of macronutrients and the Ni levels in germinating seeds were examined. METHODS Seeds were sown in glass Petri dishes and exposed to increasing concentrations of Ni (5 to 500 mg Ni L(-1)) using Ni chloride, Ni sulphate and Ni acetate. The germination percentage and root length were measured after 40 d. Longitudinal frozen sections of germinating seeds growing in the presence of Ni (500 mg L(-1) for all three salts) were used for X-ray microanalysis and X-ray elemental mapping using scanning electron microscopy (SEM). KEY RESULTS Ni chloride resulted in the greatest reductions in germination and root growth, particularly at 500 mg L(-1), followed by Ni sulphate and Ni acetate. SEM images revealed Ca crystalline structures in the seed coat for all the samples. S/Ca and Mg/P/K/Mn were found to be distributed differently in Ni-treated samples, whereas they all followed the same pattern in the controls. For all three salts, the Ni added to the medium had accumulated in the seed coat, whereas the endosperm seemed to be devoid of Ni. CONCLUSIONS It is assumed that the seed coat is able to reduce the amount of Ni entering the seed, and that a high level of Ni induced the mobilization of macronutrients.
Duodenal bacterial and nonbacterial protein supply in steers fed forage and grain diets.

Four beef steers (avg wt 300 kg) fitted with duodenal re-entrant cannulae were used to study the effect of dietary concentrate to forage ratio on bacterial and nonbacterial N flow in the duodenum. According to a change-over design, the steers were designated to receive an all forage (83% alfalfa hay and 17% wheat straw) and an 80% sorghum grain diet. Lignin (ADL) and chromium oxide (Cr2O3) ratio techniques were compared with automated total collection (ATC) of digesta for quantitating duodenal protein flow and efficiency of bacterial N yield in the rumen. Estimates of bacterial protein synthesis and ruminal escape of feed protein based on Cr2O3 and lignin tended to be higher by 8 to 16% than those obtained by ATC. Efficiency of ruminal bacterial protein yield estimated by these two markers tended to be greater than that based on ATC (16 vs 12 g of bacterial protein/100 g ruminal true digestion of dry matter corrected for bacterial cell synthesis). Efficiency values did not differ between diets. Crude protein flow into the duodenum was about 33% greater (P less than .01) for the grain than the forage diet, although protein intake was about 10% less on the grain diet. Duodenal bacterial protein, rather than feed protein escaping ruminal degradation, accounted for most of this difference. Average duodenal flow of N, expressed as g/Mcal metabolizable energy (ME) intake, was 11.9 for the forage diet and 10.3 for the grain diet. (ABSTRACT TRUNCATED AT 250 WORDS)
Platelet APP isoform ratios correlate with declining cognition in AD

Background: Platelets and neurons both contain large quantities of two carboxyl-truncated 120 to 130 and 110 kDa Alzheimer amyloid precursor proteins (APPs). Platelets taken from patients with AD have been reported to contain a reduced ratio of these APPs. Objective: To further study the AD specificity of reduced platelet APP ratios and to determine whether, after 3 years, cognitive losses in AD are accompanied by similarly reduced platelet APP ratios. Methods: To test the AD specificity of reduced platelet APP ratios, we quantitated these APPs in eight patients with PD and six patients with hemorrhagic stroke (HS). To determine whether further cognitive losses correlate with platelet APP ratio reductions in patients with AD, the authors re-examined platelet APPs and Mini-Mental State Examination (MMSE) scores of 10 patients with AD and 11 controls, who were tested 3 years ago. APP ratios were determined by the average of six assays using Western blotting with m22C11 monoclonal antibody, enhanced chemoluminescence, and digital scanning of autoradiographs. Results: APP ratios were normal in the patients with PD and HS, further supporting the AD specificity of this assay. After 3 years, the MMSE scores and APP ratios of our control subjects changed by <4%. However, the average MMSE scores of our patients with AD declined from 16.4 to 8.3, and their average 120 to 130/110 kDa APP ratios declined from 5.8 to 3.6. The difference between AD and control APP ratios, with no overlap, is significant and the correlation between the 3-year decline in AD MMSE scores and reduced APP ratios (r = 0.69) was significant. Conclusions: Although the number of subjects analyzed was limited, reduced platelet APP ratios appear to be a specific biological marker of AD and a biological index of the severity of cognitive loss in AD.
Ly-6G+CCR2− Myeloid Cells Rather Than Ly-6ChighCCR2+ Monocytes Are Required for the Control of Bacterial Infection in the Central Nervous System1

Myeloid cell recruitment is a characteristic feature of bacterial meningitis. However, the cellular mechanisms important for the control of Streptococcus pneumoniae infection remain largely undefined. Previous pharmacological or genetic studies broadly depleted many myeloid cell types within the meninges, which did not allow defining the function of specific myeloid subsets. Herein we show that besides CD11b+Ly-6G+CCR2− granulocytes, also CD11b+Ly-6ChighCCR2+ but not Ly-6ClowCCR2− monocytes were recruited in high numbers to the brain as early as 12 h after bacterial challenge. Surprisingly, CD11b+Ly-6ChighCCR2+ inflammatory monocytes modulated local CXCL2 and IL-1β production within the meninges but did not provide protection against bacterial infection. Consistent with these results, CCR2 deficiency strongly impaired monocyte recruitment to the infected brains but was redundant for disease pathogenesis. In contrast, specific depletion of polymorphonuclear granulocytes caused elevated local bacterial titer within the brains, led to an aggravated clinical course, and enhanced mortality. These findings demonstrate that Ly-6ChighCCR2+ inflammatory monocytes play a redundant role for the host defense during bacterial meningitis and that predominantly CD11b+Ly-6G+CCR2− myeloid cells are involved in the restriction of the extracellular bacteria.
Skewness and the Relation Between Risk and Return

The relationship between risk and return has been one of the most important and extensively investigated issues in the financial economics literature. The theoretical results predict a positive relation between the two. Nevertheless, the empirical findings so far have been contradictory. Evidence presented in this paper shows that these contradictions are the result of negative skewness in the distribution of portfolio excess return and the fact that the estimation of intertemporal asset pricing models are based on symmetric log-likelihood specifications.Data, as supplemental material, are available at http://dx.doi. org/10.1287/mnsc.2015. 2201 . This paper was accepted by Jerome Detemple, finance .
Organizational structure and specialized marketing capabilities in SMEs

Purpose – This paper aims to develop and test a conceptual model of organizational structure design that incorporates some factors influencing strategy implementation. The research also aims to consider inter‐functionality in new product development (NPD) processes and marketing decisions, measured from the dispersion of these activities among functional areas.Design/methodology/approach – The research was conducted across 424 small and medium‐sized furniture manufacturing companies. In total, eight hypotheses were proposed and tested using structural equation modeling.Findings – Most important among the study's findings was that inter‐firm relationships and inter‐functional processes are relevant for the study of organizational structure design. It was found that the dispersion of the new product development process and of marketing decisions exert a positive influence on architectural marketing capabilities. The results showed that the dispersion of NPD processes and marketing decisions influence the de.
Image Classification Using Naive Bayes Classifier With Pairwise Local Observations

We propose a pairwise local observation-based Naive Bayes (NBPLO) classifier for image classification. First, we find the salient regions (SRs) and the Keypoints (KPs) as the local observations. Second, we describe the discriminative pairwise local observations using Bag-of-features (BoF) histogram. Third, we train the object class models by using random forest to develop the NBPLO classifier for image classification. The two major contributions in this paper are multiple pairwise local observations and regression object class model training for NBPLO classifier. In the experiments, we test our method using Scene-15 and Caltech-101 database and compare the results with the other methods.
Gene expression study of phase I and II metabolizing enzymes in RPTEC/TERT1 cell line: application in in vitro nephrotoxicity prediction

Abstract 1. The phase I and II metabolizing enzymes of kidneys play an important role in the metabolism of xenobiotic as well as endogenous compounds and proximal tubules of kidney constitute high concentration of these metabolizing enzymes compared with the other parts. 2. It has been shown previously that differential enzyme expression among human and rodent/non-rodent species can be a roadblock in drug discovery and development process. Currently, proximal tubule cell lines of human origin such as RPTEC/TERT1 and HK-2 are used to understand the pathophysiology of kidney diseases, therapeutic efficacy of drugs, and nephrotoxicity of compounds. 3. The purpose of the present study is to understand the metabolic enzymes present in RPTEC/TERT1 and HK-2 cell lines that would help to interpret and predict probable in vitro behavior of the molecule being tested. 4. We analyzed the expression of phase I and II metabolizing enzymes of RPTEC/TERT1 and HK-2 cell lines. We found equal expression of CYP1B1, 2J2, 3A4, 3A5, UGT1A9, SULT2A1 and GSTA, higher expression of 2B6, 2D6, 4A11, 4F2, 4F8, 4F11, UGT2B7, SULT1E1 in RPTEC/TERT1 and absence of GSTT in RPTEC/TERT1 compared to HK-2 at mRNA level. Such differences can affect the outcome of in vitro nephrotoxicity prediction.
Presence of filterable and nonfilterable cell-free mRNA in amniotic fluid.

Much current research focuses on the properties and clinical applications of circulating nucleic acids (1). The recent discovery of cell-free RNA in the plasma and serum of cancer patients has generated considerable interest (2)(3)(4)(5)(6). Circulating RNA is surprisingly stable, and Ng et al. (7) recently showed that a considerable proportion of plasma mRNA species is particle associated and thus possibly protected from nuclease degradation (8). Fetal-derived mRNA has also been found in the plasma and serum of pregnant women (9)(10)(11) and in amniotic fluid (12), and has many potential clinical applications (13)(14). Amniotic fluid is routinely collected during amniocentesis for fetal chromosome analysis or fetal lung maturity studies. However, little is known regarding the biology of circulating fetal mRNA or fetal mRNA in amniotic fluid. In this report, we explore whether cell-free fetal nucleic acids in amniotic fluid have properties similar to circulating DNA and mRNA. Expanding on the work of Ng et al. (7), we hypothesized that cell-free fetal mRNA in amniotic fluid might be present in a particle-associated form and could thus be filterable, whereas the non-particle–associated form of DNA would be present in such high concentrations that there would be no significant reduction in its quantity by filtration. Additionally, we wished to compare the quantities of nucleic acids in amniotic fluid containing cells with the quantities in filtered and unfiltered cell-free supernatant. We hypothesized that whole amniotic fluid containing amniocytes would contain a significantly larger amount of DNA and RNA than cell-free amniotic fluid. This study was performed with Institutional Review Board approval from Tufts-New England Medical Center. Seven amniotic fluid samples with a minimum volume of 3 mL each were obtained from women undergoing scheduled amniocenteses. One sample originated from a woman …
RUNX3 expression in primary and metastatic pancreatic cancer

Aim: Runx transcription factors are important regulators of lineage specific gene expression, cell proliferation, and differentiation. Runx3 expression is lost in a high proportion of gastric cancers, suggesting a tumour suppressive role in this malignancy. This study investigates the expression and localisation of Runx3 in pancreatic tissues. Methods: Quantitative polymerase chain reaction was used to measure Runx3 mRNA. Immunohistochemistry was carried out to localise Runx3 in normal pancreatic tissues, and in primary and metastatic pancreatic ductal adenocarcinoma (PDAC). Basal and transforming growth factor β1 (TGFβ1) induced Runx3 expression was analysed in cultured pancreatic cancer cell lines. Results: Runx3 expression was low to absent in normal pancreatic tissues, but increased in a third of cancer tissues. Runx3 was present only in islets in normal pancreas, whereas in pancreatic cancers, Runx3 was detected in the cancer cells of seven of 24 samples analysed. In addition, it was expressed by lymphocytes in six of the 16 cases with lymphocyte infiltration. In pancreatic cancer cell lines, Runx3 mRNA was present in Colo-357 and T3M4 cells, but was low to absent in the other cell lines tested. TGFβ1 repressed Runx3 mRNA expressed in Colo-357 cells, and had no effect on Runx3 expression in the other pancreatic cancer cell lines. Conclusion: Runx3 expression is restricted to islets in the normal pancreas. In contrast, a considerable proportion of pancreatic tumours express Runx3, and its expression is localised in the tumour cells and in the infiltrating lymphocytes. Thus, Runx3 might play a role in the pathogenesis of PDAC.
Retinal Nerve Fiber Layer Thickness Variability in Leber Hereditary Optic Neuropathy Carriers

Purpose. Recent investigations suggested that unaffected carriers of Leber hereditary optic neuropathy (LHON) may show subclinical visual alterations. Structural changes have also been detected by optical coherence tomography (OCT), which revealed a temporal thickening of the retinal nerve fiber layer (RNFL). These changes may reflect compensatory effects such as mitochondria accumulation within the RNFL axons. This study aimed to investigate whether the RNFL of LHON carriers shows greater than expected thickness variations, which may reflect transient subclinical changes, over the course of years. Methods. Using Stratus OCT, the RNFL thickness was measured yearly from 2005 to 2008 in 24 Brazilian LHON carriers, all with homoplasmic 11778/ND4 mtDNA mutation. An Italian sample of 20 healthy subjects served as a control. Data were compared also to a previously published sample (n=59) of glaucomatous eyes. Results. The LHON carriers showed test-retest standard deviations that were larger than normal controls in the temporal (p=0.004), superior (p<0.0001), and inferior quadrants (p=0.019). Compared to the glaucoma cases, no statistical differences were observed. Conclusions. The RNFL thickness in LHON carriers, when measured at different time points, has higher variability than in normal subjects. Transitory RNFL swelling may be caused either by compensatory mechanisms (increased mitochondrial biogenesis) or by axonal stasis preceding decompensation of retinal ganglion cells. In both situations, these changes may represent the origin of the visual alterations previously detected in LHON carriers. Alternatively, increased variability of RNFL thickness may be influenced by the LHON microangiopathy, as retinal blood vessels contribute to the OCT RNFL thickness measurements.
Assessment of novel combinations of biomarkers for the detection of colorectal cancer.

BACKGROUND Patients with colorectal cancer often present with advanced disease and concomitant poor prognosis. The best known serum biomarker, carcinoembryonic antigen (CEA) is not recommended for screening because of its limited specificity and sensitivity. A number of other circulating proteins have been suggested to be diagnostically useful but individually none of these has proved to be of sufficient sensitivity or specificity to establish a role in routine clinical practice. Here, we test the hypothesis that combining several of these biomarkers will improve diagnostic efficacy. METHODS To select the markers for our model we screened CEA and 26 other candidate biomarkers. Four candidates were selected and their concentrations determined in the serum of 239 patients (106 colorectal cancer patients and 133 non-cancer subjects). RESULTS Class prediction models based on CEA, DR-70 and sCD26 produced a modest increase in detection accuracy over CEA alone, particularly for early stage cancers. The sensitivity and specificity required for a clinically useful test was not reached. CONCLUSION It is unlikely that a biomarker panel comprised of the currently available serum markers will generate a clinically useful diagnostic test for colorectal cancer. Our findings reiterate the urgent need to discover novel biomarkers for the detection of colorectal cancer.
Uniformly Tensile-Strained Germanium Enabled by a Recessed Nitride Stressor for Efficient Integrated Photodetectors at Longer Wavelengths

Germanium photodetector, formed with a self-aligned dry etching method, together with a tensile silicon nitride sidewall stressor, exhibits a strain profile with improved uniformity and a $\sim 2\times$ enhancement on the quantum efficiency at the $L$ -band, © 2019 The Author(s)
1 CYP 1 A 2 polymorphism-1545 C > T ( rs 2470890 ) is associated with side effects of cl zapine

University of Tampere, Medical School, 33014 University of Tampe re, Finland Tampere Mental Health Centre, Hallituskatu 8 B, 33200 Tampere, Finland Seinäjoki Hospital District, Department of Psychiatry, Seinäjoki, Finla nd. Department of Psychiatry, Satakunta Hospital District, Fi-28500 Pori , Fin land Department of clinical chemistry, Fimlab Laboratories, School f Medicine University of Tampere, Tampere, Finland Tampere University Hospital, Department of Psychiatry, Tampere, Finl and
Fixed-order output feedback controller design for discrete-time T-S fuzzy affine systems using quantized measurements

This paper addresses the problem of robust fixed-order dynamic output feedback (DOF) control for a class of Takagi-Sugeno (T-S) fuzzy affine systems via utilizing quantized measurements. Through a state-input augmentation method and piecewise quadratic Lyapunov functions, some sufficient conditions for fixed-order piecewise affine DOF controller synthesis are developed. It is noted that by some convexification techniques and S-procedure, sufficient conditions for the DOF controller synthesis are obtained in terms of a set of linear matrix inequalities (LMIs). One illustrative simulation example is shown to verify the effectiveness of the proposed controller design approach.
Class I Lysine Deacetylases Facilitate Glucocorticoid-induced Transcription*

Background: KDACis impair GR transactivation of the MMTV promoter, but their impact on cellular target genes is unknown. Results: KDACi or KDAC depletion suppresses transactivation of about 50% of GR target genes. Conclusion: KDAC1 is required for efficient GR transactivation in a gene-selective fashion. Significance: Because KDACs facilitate GR transactivation, clinical KDACi use may have a major impact on GR signaling. Nuclear receptors use lysine acetyltransferases and lysine deacetylases (KDACs) in regulating transcription through histone acetylation. Lysine acetyltransferases interact with steroid receptors upon binding of an agonist and are recruited to target genes. KDACs have been shown to interact with steroid receptors upon binding to an antagonist. We have shown previously that KDAC inhibitors (KDACis) potently repress the mouse mammary tumor virus promoter through transcriptional mechanisms and impair the ability of the glucocorticoid receptor (GR) to activate it, suggesting that KDACs can play a positive role in GR transactivation. In the current study, we extended this analysis to the entire GR transcriptome and found that the KDACi valproic acid impairs the ability of agonist-bound GR to activate about 50% of its target genes. This inhibition is largely due to impaired transcription rather than defective GR processing and was also observed using a structurally distinct KDACi. Depletion of KDAC1 expression mimicked the effects of KDACi in over half of the genes found to be impaired in GR transactivation. Simultaneous depletion of KDACs 1 and 2 caused full or partial impairment of several more GR target genes. Altogether we found that Class I KDAC activity facilitates GR-mediated activation at a sizable fraction of GR-activated target genes and that KDAC1 alone or in coordination with KDAC2 is required for efficient GR transactivation at many of these target genes. Finally, our work demonstrates that KDACi exposure has a significant impact on GR signaling and thus has ramifications for the clinical use of these drugs.
Clinical and Microbiological Characteristics of Community-Acquired Staphylococcus lugdunensis Infections in Southern Taiwan

ABSTRACT Most Staphylococcus lugdunensis strains (49/59, 83%) were related to clinical infections, were susceptible to most antimicrobial agents with an overall oxacillin-resistant rate of 5% (3/58), and carried relatively great genetic diversity. Community-acquired infections (41/49, 84%) were dominant, often developed in patients with comorbidity, and had rather benign clinical courses without mortality.
Shear Melting and High Temperature Embrittlement: Theory and Application to Machining Titanium

We show that alloying with rare earth metals (REMs) can dramatically improve the machineability of a range of titanium alloys, even though the REM is not incorporated in the alloy matrix. The mechanism for this is that under cutting, shear bands are formed within which the nano-precipitates of REM are shear mixed. This lowers the melting point such that the mechanism of deformation changes from dislocation mechanism to localised amorphisation and shear softening. The material then fractures along the thin, amorphous shear-band. Outside the shear band, the REM remains as precipitates. The new alloys have similar mechanical properties and biocompatibility to conventional materials.