Miracle-dz/UniFAR-FTUs huggingface dataset:
Reduction of radiation risks in patients undergoing some X-ray examinations by using optimal projections: A Monte Carlo program-based mathematical calculation

The objectives of this paper were calculation and comparison of the effective doses, the risks of exposure-induced cancer, and dose reduction in the gonads for male and female patients in different projections of some X-ray examinations. Radiographies of lumbar spine [in the eight projections of anteroposterior (AP), posteroanterior (PA), right lateral (RLAT), left lateral (LLAT), right anterior-posterior oblique (RAO), left anterior-posterior oblique (LAO), right posterior-anterior oblique (RPO), and left posterior-anterior oblique (LPO)], abdomen (in the two projections of AP and PA), and pelvis (in the two projections of AP and PA) were investigated. A solid-state dosimeter was used for the measuring of the entrance skin exposure. A Monte Carlo program was used for calculation of effective doses, the risks of radiation-induced cancer, and doses to the gonads related to the different projections. Results of this study showed that PA projection of abdomen, lumbar spine, and pelvis radiographies caused 50%-57% lower effective doses than AP projection and 50%-60% reduction in radiation risks. Also use of LAO projection of lumbar spine X-ray examination caused 53% lower effective dose than RPO projection and 56% and 63% reduction in radiation risk for male and female, respectively, and RAO projection caused 28% lower effective dose than LPO projection and 52% and 39% reduction in radiation risk for males and females, respectively. About dose reduction in the gonads, using of the PA position rather than AP in the radiographies of the abdomen, lumbar spine, and pelvis can result in reduction of the ovaries doses in women, 38%, 31%, and 25%, respectively and reduction of the testicles doses in males, 76%, 86%, and 94%, respectively. Also for oblique projections of lumbar spine X-ray examination, with employment of LAO rather than RPO and also RAO rather than LPO, demonstrated 22% and 13% reductions to the ovaries doses and 66% and 54% reductions in the testicles doses, respectively.
Scanning Laser Polarimetry in Monkey Eyes using Variable Corneal Polarization Compensation

PurposeTo determine if scanning laser polarimetry (SLP) using variable anterior segment birefringence compensation can provide meaningful retinal nerve fiber layer (RNFL) thickness measurements in monkey eyes. MethodsA scanning laser polarimeter (GDx; Laser Diagnostic Technologies, San Diego, CA) was modified so that anterior segment birefringence could be compensated on an eye-specific basis. Six eyes of three adult Cynomolgus (Macaca fascicularis) monkeys were imaged. The authors determined the corneal polarization magnitude (CPM) and corneal polarization axis (CPA) in these eyes, and compared them with the fixed values in the commercial scanning laser polarimeter. Individually compensated RNFL images, using eye-specific CPM and CPA, were then obtained to determine if the resulting retardation profiles reflected the expected RNFL appearance observed with stereoscopic optic disc photographs. Two of the imaged monkeys had experimental glaucoma of the right eye, which allowed comparison of RNFL thickness measures between healthy eyes and those damaged by experimental glaucoma. ResultsThe CPM was small in each of the six eyes examined, ranging from 5.7 to 8.7 nm. The CPA ranged from −62° to 78.7° (nasally upward CPA values were recorded as negative; nasally downward CPA values were recorded as positive). These values are different from the values assumed by the commercially available fixed-compensator GDx. When eye-specific compensation was used, RNFL retardation profiles mimicked the expected appearance of the RNFL in all eyes. The authors also observed a substantial decrease in retardation in experimental glaucoma eyes compared with healthy fellow eyes. ConclusionsScanning laser polarimetry using eye-specific corneal polarization compensation can provide meaningful RNFL thickness measurements in monkey eyes. Observed differences in retardation between healthy and experimental glaucoma eyes suggest that SLP may be useful for detecting and monitoring RNFL loss in experimental primate glaucoma.
Corticopontine projection in the macaque: The distribution of labelled cortical cells after large injections of horseradish peroxidase in the pontine nuclei

We studied the distribution of corticopontine cells in the monkey cerebral cortex. Horseradish peroxoidase (HRP) was injected into the brainstem of monkeys in an attempt to fill the pontine nuclei on one or both sides. In control animals we injected the medullary pyramids or varied the route, size, or location of pontine injections. All retrograde filled corticopontine neurons were layer V pyramidal cells. Corticopontine cells were distributed within a largely continuous area of cortex which extended from the cingulate cortex medially to the sylvian fissure laterally; from the superior temporal fissure caudally to the medial part of the frontal granular cortex rostrally. Areas 4 and 6 of Brodmann (1905) contained the highest density of filled cells. In the primary visual cortex, area 17, there were a few labelled cells restricted to the rostral portion of the upper bank of the calcarine fissure, in a region representing the lower periphery of the visual field. The results are discussed in relation to the possible functions of the corticopontine system, especially the role of the extrastriate visual areas in visually guided movement.
CO2 Stimulation of the Cornea: A Comparison Between Human Sensation and Nerve Activity in Polymodal Nociceptive Afferents of the Cat

Excitation of nociceptors by low pH has been proposed as a cause of pain following tissue injury. Here we have studied the effect of pH reductions caused by application of CO2 pulses to the cornea on the activity of corneal afferent nerves of the cat and on the magnitude of pain sensations in humans. Single‐unit activity was recorded from corneal afferent fibres in anaesthetized cats. The corneal receptive field of A‐delta or C polymodal nociceptive units was exposed for 30 s to a gas mixture with different concentrations of CO2 in air (0, 35, 50, 65, 80 and 98.5%). Responses to CO2 were evoked at a mean threshold concentration of 40 ± 3% CO2. They consisted of a discharge of impulses that decayed gradually to a tonic level. In 15% of the units the initial burst was absent. The CO2 concentration and firing frequency data could be fitted to a power function with an exponent of 1.12. Pulses of CO2 were also applied to the cornea of 16 human volunteers. Sensations experienced were measured by means of a visual analogue scale and a verbal descriptor scale. Flow was adjusted below the mechanical stimulation threshold (2.8 ± 0.5 mg). When mixtures containing 10‐90% CO2 in 5% steps were applied as 3 s pulses, threshold sensation, described as a mild stinging pain, was evoked at 33.5 ± 4.0% CO2. This sensation became overtly painful with higher CO2 concentrations (47.5 ± 3.6% CO2). For the same subject the sensory threshold was remarkably constant, though it changed with longer exposure times. The relationship between CO2 concentration and magnitude of pain could be adjusted to a power function with a power exponent of 1.12. Curves of CO2 concentration versus neural discharges in the cat and versus psychophysical sensation in humans were very similar. These results show that corneal polymodal nociceptors respond to protons, and encode changes in CO2 concentration presumably reflecting pH changes. The same stimulus evokes corneal pain sensations in humans, thus opening the possibility of using CO2 as an effective stimulus for corneal aesthesiometry.
Diversity and dynamics of the DNA- and cDNA-derived compost fungal communities throughout the commercial cultivation process for Agaricus bisporus

ABSTRACT Commercial cultivation of the button mushroom Agaricus bisporus is performed through the inoculation of a semipasteurized composted material. Pasteurization of the compost material prior to inoculation results in a substrate with a fungal community that becomes dominated by A. bisporus. However, little is known about the composition and activity in the wider fungal community beyond the presence of A. bisporus in compost throughout the mushroom cropping process. In this study, the fungal cropping compost community was characterized by sequencing nuc rDNA ITS1-5.8S-ITS2 amplified from extractable DNA and RNA. The fungal community generated from DNA extracts identified a diverse community containing 211 unique species, although only 51 were identified from cDNA. Agaricus bisporus was found to dominate in the DNA-derived fungal community for the duration of the cropping process. However, analysis of cDNA extracts found A. bisporus to dominate only up to the first crop flush, after which activity decreased sharply and a much broader fungal community became active. This study has highlighted the diverse fungal community that is present in mushroom compost during cropping.
Male breast cancer in north eastern Nigeria.

BACKGROUND Carcinoma of the male breast is generally rare and constitutes 1% of all breast cancers. They often present late in developing countries and therefore has poor prognosis. The aim of this paper is to highlight the pattern of presentation and problems associated with management of this disease in Maiduguri, North Eastern Nigeria. PATIENTS AND METHOD Case records of eleven (11) histologically diagnosed male breast cancers out of a total of two hundred and ninety-five (295) cases of breast cancer managed at the University of Maiduguri Teaching Hospital between 1989-2003 were retrospectively studied and analysed. RESULTS Male breast cancer constitutes 3.7% of all cases of breast cancers seen in this hospital during the study period under review. The male to female ratio was 1:26. The peak age range at presentation was 40-49 years and all were advanced at presentation. The time interval between onset of symptoms and presentation were all over 12 months. All the patients either had toilet mastectomy or modified radical mastectomy. In addition, two patients had sub capsular orchidectomy while nine had Tamoxifen. Four patients had additional cytotoxic chemotherapy. Two patients died in the course of follow-up while the rest were lost to follow-up shortly after discharge. Only one patient was followed-up for more than two years. CONCLUSION Male breast cancer though rare, is a serious clinical problem associated with late presentation. It is hoped that increased public awareness will improve the outcome of management.
Analysis of the upstream activating sequence and site of carbon and nitrogen source repression in the promoter of an early-induced sporulation gene of Bacillus subtilis

The transcription from the spoVG promoter of Bacillus subtilis is induced at the start of the stationary phase of growth and is dependent on the expression of the spoOA, spoOB, and spoOH genes. It is repressed in cells grown in the presence of excess glucose and glutamine and is under the negative control of the abrB gene. The spoOA and spoOB gene products function to suppress the negative control exerted by abrB. Transcription initiation requires the form of RNA polymerase holoenzyme that contains the spoOH gene product, sigma H. Optimal transcription also requires an upstream A-T-rich region termed the upstream activating sequence (UAS). The mechanism of UAS function was examined through mutational analysis of the spoVG promoter region. Deletion of the UAS or positioning the UAS one half turn or one full turn of the DNA helix upstream of its location in wild-type spoVG resulted in a severe reduction in promoter activity. Deletion of most of the UAS abolished the abrB-dependent repression of spoVG transcription. Higher activity was observed when the UAS was inserted 10 bp (one turn of the helix) upstream than when the sequence was repositioned either 5 or 13 bp upstream. Sequences upstream of the UAS were found not to be involved with the position-dependent function of the UAS. Positioning the UAS 42 or 116 bp upstream eliminated the stimulatory effect of the sequence on spoVG transcription. These data indicate that the UAS functions effectively when it is in close proximity to the -35 region. In vitro transcription analysis indicated that the deletion and insertion mutation affecting the UAS impair RNA polymerase-spoVG promoter interaction. Deletion of the UAS showed that the negative effect of exogenous glucose and glutamine is not dependent on the UAS but is exerted at a site within or near the -35 and -10 regions.
L-arginine deficiency and supplementation in experimental acute renal failure and in human kidney transplantation.

BACKGROUND The "L-arginine paradox" refers to situations where L-arginine (L-Arg) supplementation stimulates nitric oxide (NO) synthesis, despite saturating intracellular concentrations. This paradox is frequently observed in acute renal failure (ARF). First, the effects of L-Arg on renal function of rats with ARF were studied. Based on the promising results from these initial studies, the second part of our study searched for a form of ARF in humans that could be studied easily under conditions with little variance and yet was linked with endothelial dysfunction. Thus, we investigated the effects of L-Arg supplementation immediately after kidney transplantation in 54 patients. METHODS In uranyl nitrate-induced ARF in rats the effects of L-Arg and L-NNA (inhibitor of nitric oxide synthase; NOS) on glomerular filtration rate (GFR), renal plasma flow (RPF), blood pressure (BP) and NOx (NO2- +NO3-) excretion were examined. Tissue L-Arg levels, NOS activities, immunodetection of NOS and superoxide dismutase (SOD), activities of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and xanthine oxidase, and nitrotyrosine immunoreactive protein (NT-IR) were determined and compared to sham operated animals. Secondly, in a randomized, double-blind study, the effects of L-Arg on GFR and RPF were investigated in 54 kidney transplant recipients, receiving IV L-Arg for three days. GFR and RPF were measured on days 1, 3, 5 and 10 by scintigraphy. RESULTS In experimental ARF, decreased RPF and GFR were associated with reduced tissue L-Arg levels, endothelial NOS-III expression, NO formation and NOx excretion. Reduction in GFR, RPF and NOx excretion were reversed upon administration of exogenous L-Arg. There also was a loss of Cu,Zn-SOD, a key enzyme against oxidative stress, and an elevation of NT-IR, an indicator of nitrosative stress and suggested marker for pathological actions of NO. However, NT-IR was not dependent on de novo NO synthesis and not related to the functional effects of l-Arg administration. In kidney transplant recipients receiving organs with a short cold ischemia time (CIT) and from young donors, that is, those with a higher likelihood of a functional endothelium, early administration of L-Arg improved renal function. CONCLUSION Both experimental and clinical data show that \L-Arg deficiency and endothelial dysfunction are pathomechanistically relevant in ARF. The data suggest a therapeutic potential for the administration of L-Arg in ARF and kidney transplantation, at least in patients receiving kidneys with shorter CIT and from younger donors.
Design and demonstration of 1-bit and 2-bit transmit-arrays at X-band frequencies

This article describes the design of planar transmit-arrays at 10 GHz with 1-bit and 2-bit phase quantization and specific unit-cell designs in each case. The influence of the main design parameters are investigated numerically. The measured directivity/gain values equal 26/23.6 dBi for the 1-bit design, and 23.7/14.3 dBi for the 2-bit design, respectively. In particular, the influence of the unit-cell size is highlighted. Both designs are built on a two-layer printed circuit board assembly and operate in linear polarization. Beam-steering characteristics up to ±30° are investigated and compared by tilting the feed source and by changing the phase distribution across the array.
Cellular Gene Expression That Correlates with EBER Expression in Epstein-Barr Virus-Infected Lymphoblastoid Cell Lines

ABSTRACT Novel Epstein-Barr Virus (EBV) strains with deletion of either EBER1 or EBER2 and corresponding revertant viruses were constructed and used to infect B lymphocytes to make lymphoblastoid cell lines (LCLs). The LCLs were used in microarray expression profiling to identify genes whose expression correlates with the presence of EBER1 or EBER2. Functions of regulated genes identified in the microarray analysis include membrane signaling, regulation of apoptosis, and the interferon/antiviral response. Although most emphasis has previously been given to EBER1 because it is more abundant than EBER2, the differences in cell gene expression were greater with EBER2 deletion. In this system, deletion of EBER1 or EBER2 had little effect on the EBV transformation frequency of primary B cells or the growth of the resulting LCLs. Using the recombinant viruses and novel EBER expression vectors, the nuclear redistribution of rpL22 protein by EBER1 in 293 cells was confirmed, but in LCLs almost all of the cells had a predominantly cytoplasmic expression of this ribosomal protein, which was not detectably changed by EBER1. The changes in LCL gene expression identified here will provide a basis for identifying the mechanisms of action of EBER RNAs.
Rectenna design and optimization using reciprocity theory and harmonic balance analysis for electromagnetic (EM) energy harvesting

A rectenna design methodology combining electromagnetic (EM) simulation and harmonic balance (HB) analysis is presented. It consists of applying reciprocity theory to calculate the Thevenin equivalent circuit of the receiving antenna and optimizing the rectifying circuit parameters using HB analysis. The method is demonstrated by designing a 2.45-GHz rectenna based on a square aperture-coupled patch antenna with dual linear polarization. A compact implementation is achieved by etching a cross-shaped slot on the patch surface leading to a 32.5% patch side reduction. Voltage-doubling circuits convert the received RF power from each port to dc permitting the rectenna to receive arbitrarily polarized signals. The circuit is optimized for low input power densities and a simulated maximum efficiency of 38.2% was obtained for 1.5 nWcm-2 input RF power density at 2.43 GHz.
Natural History and Molecular Characteristics of Lung Cancers Harboring EGFR Exon 20 Insertions

Introduction: Exon 20 insertions are the third most common family of epidermal growth factor receptor (EGFR) mutations found in non–small-cell lung cancer (NSCLC). Little is known about cancers harboring these mutations aside from their lack of response to EGFR tyrosine kinase inhibitors, impairing the development of effective targeted therapies. Methods: NSCLC patients with EGFR genotyping were studied using a mechanism approved by the Institutional Review Board. Cancers with exon 20 insertions were indentified, sequences were characterized, and effectiveness of different treatment regimens was reviewed retrospectively. Clinical characteristics and survival were compared with cancers harboring common EGFR mutations and cancers with wild-type EGFR. Results: One thousand eighty-six patients underwent EGFR genotyping from 2004 to 2012. Twenty seven (2.5%) harbored exon 20 insertions, making up 9.2% of all cancers with documented EGFR mutations. Compared with wild-type cancers, those with exon 20 insertions were more commonly found in never-smokers and Asian patients. Insertion sequences were highly variable, with the most common variant (V769_D770insASV) making up only 22% of cases. Median survival of patients with exon 20 insertions was 16 months, similar to the survival of wild-type cancers and shorter than the survival of cancers with common EGFR mutations. Conclusions: Patients with EGFR exon 20 insertions have similar clinical characteristics to those with common EGFR mutations but a poorer prognosis. The prevalence of this subset of NSCLC is similar to that of other genotype-defined subsets of lung adenocarcinoma (e.g. those with BRAF mutations, HER2 insertions, ROS1 rearrangements) and is a population of interest for trials of new targeted therapies.
Gastric secretion pH measurement: What you see is not what you get!

We evaluated the accuracy of gastric secretion pH measurements as performed in three ICUs. The pH of 275 samples was measured with pH paper using established techniques. The pH of 85 additional samples was determined with a hand-held pH meter. All specimens also were measured using a research laboratory pH meter to learn the true pH. Analyses included mean and so of the difference between the two measurements, the correlation coefficient (r value), and the concordance correlation coefficient. The pH meter values disagreed significantly with pH paper measurements. Measurements of gastric secretion pH with pH indicator paper do not guide therapy reliably. Inaccurate values derived from pH paper measurements could have resulted in inappropriate treatment in 28% of the samples tested. A portable, battery-powered pH meter accurately reproduced laboratory pH meter measurements and is a reasonable device for clinical use. (Crit Care Med 1990; 18:396)
Mechanics of the Middle Ear System: Age-Related Changes in Viscoelastic Properties

Morphological studies of age-related changes in the tympanic membrane have suggested a decreased elasticity for increasing age, whereas age-related changes in admittance measured by tympanometry have revealed inconsistent results. In this study we report on the viscoelastic properties of the middle ear system by means of a new method in two different age groups. This method measures hysteresis (μJ), which describes the viscous properties of the system, and compliance (mm3/kPa) reflecting its elasticity. Moreover, Pec0 (kPa) was recorded, which corresponds to middle ear pressure measured by tympanometry. Recordings were made in a group of 30 older normal subjects (mean age 77 years) and results were compared to previous findings in a group of 39 younger normal subjects (mean age 29). Hysteresis was markedly decreased in the older normal group compared to the younger one (p = 0.057). This may reflect morphological changes previously reported in the tympanic membrane and can be related to theoretical considerations on tympanic membrane rupture pressures. Compliance and Pec0 were not influenced by variation in age (p = 0.645 and 0.966).
VOCAL TRACT EFFECTS ON THE TIMBRE OF THE SAXOPHONE

For notes sounded over the normal and altissimo playing range, experienced saxophonists can produce changes in the spectral envelope of the radiated sound by adjusting their vocal tract configuration. Measurements of the vocal tract acoustic impedance, Zmouth, during performance showed that, when Zmouth was comparable with the input impedance of the bore, Zbore, i.e. several MPa∙s∙m or more, harmonics of the radiated sound falling near these peaks in Zmouth were substantially enhanced. In contrast, the broadband noise in the radiated sound produced by upstream turbulence was attenuated in the frequency range over which the magnitude of Zmouth was large.