Miracle-dz/UniFAR-FTUs huggingface dataset:
Analysis of the Binding Forces Driving the Tight Interactions between β-Lactamase Inhibitory Protein-II (BLIP-II) and Class A β-Lactamases*

β-Lactamases hydrolyze β-lactam antibiotics to provide drug resistance to bacteria. β-Lactamase inhibitory protein-II (BLIP-II) is a potent proteinaceous inhibitor that exhibits low picomolar affinity for class A β-lactamases. This study examines the driving forces for binding between BLIP-II and β-lactamases using a combination of presteady state kinetics, isothermal titration calorimetry, and x-ray crystallography. The measured dissociation rate constants for BLIP-II and various β-lactamases ranged from 10−4 to 10−7 s−1 and are comparable with those found in some of the tightest known protein-protein interactions. The crystal structures of BLIP-II alone and in complex with Bacillus anthracis Bla1 β-lactamase revealed no significant side-chain movement in BLIP-II in the complex versus the monomer. The structural rigidity of BLIP-II minimizes the loss of the entropy upon complex formation and, as indicated by thermodynamics experiments, may be a key determinant of the observed potent inhibition of β-lactamases.
A liver nodule in a patient transplanted for primary sclerosing cholangitis: an interdisciplinary diagnostic approach

Abstract We report the case of a 53-year-old female patient who was transplanted with the liver of a 71-year-old male donor for advanced primary sclerosing cholangitis (PSC) and who additionally was diagnosed with a histologically non-classifiable colitis shortly before transplantation. Upon follow-up abdominal ultrasound 4 months after transplantation, a liver lesion measuring 16 × 23 mm was detected in the transplanted liver. This lesion had not been noticed immediately after transplantation and showed a pattern suspicious for malignancy in contrast-enhanced ultrasound. In line, a biopsy revealed the presence of a metastasis of an adenocarcinoma of colorectal origin, suggesting that a colitis- and PSC-associated colorectal cancer of the recipient might have been overseen upon the initial diagnostic workup. Despite two negative follow-up colonoscopies, this hypothesis was further supported by a strong positive signal in projection to the cecum in a subsequently performed PET/CT-scan. However, surgical resection of the right colon that was performed simultaneously with the atypical resection of the liver metastasis only revealed an inflamed diverticulum but no malignancy in the resected colon segment. Moreover, cytogenetic and molecular genetic testing on the resected specimens clearly attributed the metastasis to the male donor. On the one hand, this case underlines the necessity of endoscopic surveillance of patients with PSC and/or inflammatory bowel disease as well as the challenges in diagnosis of colitis-associated cancer. On the other hand, it shows that the acceptance of organs from elderly donors in times of organ shortage might be linked to an increased risk of donor transmitted malignancies.
Use of Glycopeptides at a French Teaching Hospital

The recently published guideline for the use of alcohol hand antisepsis states that "If hands are not visibly soiled, use an alcohol-based hand rub for routinely decontaminating hands (IA). Alternatively, wash hands with an antimicrobial soap . (IB). " Systematic disinfection before entering and leaving a patient's room complies with this guideline and, as recommended, might be a good educational means to improve hand washing, as noted by Voss et al. In the same guideline, the technique recommended for hand disinfection is stated: "When decontaminating hands with an alcohol-based hand rub, apply product to palm of one hand and rub hands together, covering all surfaces of hands and fingers, until hands are dry (IB). " No mention is made of time or of the quantity of alcohol to be used because too few data are given in the literature. The guideline concludes, "The efficacy of alcoholbased hand-hygiene products is affected by several factors, including the type of alcohol used, concentration of alcohol, contact time, volume of alcohol used, and whether the hands are wet when the alcohol is applied. The ideal volume of product to apply to the hands is not known and may vary for different formulations. "
Morphometric studies of the Sertoli cells in elderly men with special reference to the histology of the prostate. An analysis in an autopsy series.

An index (Si) for the absolute number of Sertoli cells, based upon the count of Sertoli cell nuclei, was determined in a consecutive autopsy series of 141 men over 40 years of age. This investigation formed part of a comprehensive study of morphological characteristics of the testes, the pituitary gland and the adrenal glands in men with prostatic hyperplasia and neoplasia. Patients with clinically manifest carcinoma of the prostate or who had previously been subjected to prostatic surgery were not included. Si varied widely at all ages and within all groups of prostatic histology. At both simple and multiple regression analyses, Si showed a negative and statistically significant correlation to age. The number of Sertoli cells per se was not clearly related to the histology of the prostate. In men with normal histology of the prostate, who were all under 70 years of age, Si remained practically unaltered with advancing age. Declining Sertoli cell numbers with age were demonstrated in patients with benign nodular hyperplasia either alone (BNH) or together with carcinoma (C + BNH), although the correlation was statistically significant only within the BNH group.
Self-management in chronic conditions: partners in health scale instrument validation.

AIMS This article describes a study that aimed to validate the Self-care in Chronic Conditions Partners in Health Scale instrument in the Mexican population. The instrument has been validated in Australia for use as a screening tool by primary healthcare professionals to assess the self-care skills and abilities of people with a chronic illness. METHODS Validation was conducted using baseline data for 552 people with diabetes, hypertension and cancer aged 18 or older who were users of healthcare centres in Tampico, Tamaulipas, Mexico. RESULTS Results show high reliability and validity of the instrument and three themes were identified: knowledge, adherence, and dealing with and managing side effects. CONCLUSION The findings suggest the scale is useful as a generic self-rated clinical tool for assessing self-management in a range of chronic conditions, and provides an outcome measure for comparing populations and change in patient self-management knowledge and behaviour. The authors recommend validating the scale in other Latin-American settings with more research into the effect of gender on self- management.
Towards an Open-Source Web Security Survey Applicable University Students

This work makes two contributions: (1) we propose the novel idea of using an open-source approach to writing surveys, and (2) we give a first step towards applying this approach in the context of surveying students about their web security literacy. Questions that interest us include: Does taking a course in security help students be more aware of the risks and vulnerabilities in their web browsing practices? When presented with a security warning, do they make the right decision? We present our results in designing and applying a survey on web security at a large university. We have released our survey instrument to the public domain and are in the process of getting other institutions to help refine the questions. We envision the following advantages of open-sourcing surveys: questions can be re-used at different institutions, results can be compared between institutions, and a world-wide community can collaborate and improve the quality of the survey instrument over time.
Evaluation of knowledge management system using technology acceptance model

This study was motivated to assess the Knowledge Management System (KMS) using Technology Acceptance Model (TAM). TAM is the best concept to be taken as model on explaining user attitude of new technology. TAM model used in the study because it has been widely adopted among IT researchers and appears to be growing rapidly, has the reliability and construct validity were established, and realized that the model has not been applied to the acceptance KMS. The data population in this study is the employees in PT. XYZ who have work-related to development and maintenance process. The data analysis was done using Partial Least Squares (PLS). The analysis was proof to be statistically significant: a) perceived ease of use and perceived usefulness, b) perceived ease of use and attitude, c) perceived usefulness and attitude, d) perceived usefulness and behavioral intention to use, e) behavioral intention to use and actual use.
Optimal Control Strategies for an SSVEP-Based Brain-Computer Interface

We evaluate the performance of 18 healthy subjects on a steady-state visually evoked potential brain–computer interface (BCI) under variation of two general control parameters. The BCI is a simple game amenable to performance measures such as the bitrate, decision accuracy, and optimality ratios based on an ideal human–machine system. The two parameters studied are the electroencephalography recording history length used to form a decision and the number of consecutive identical decisions that must be recognized before feedback is provided. To maximize the bitrate, it appears optimal to minimize the number of consecutive identical decisions required for feedback. When the task of interest often requires making the same decision multiple times in a row, a larger history of data seems preferable. When good performance on a task demands that decisions change rapidly, a smaller history seems optimal. Ultimately, we plan to connect this work to choosing appropriate control parameters for efficient wheelchair control by a BCI.
Sexual Abuse Victimization and Psychological Distress Among Adolescent Offenders

ABSTRACT This study focused on sexual abuse victimization and psychological distress among 272 adolescent offenders. The respondents were interviewed while they were being detained in a short-term holding facility. Female respondents reported more sexual abuse victimization and psychological distress than did their male counterparts. Furthermore, church attendance moderated the association between sexual abuse victimization and psychological distress among the male respondents. Implications of these findings for research and interventions with adolescent offenders are discussed.
Remarkable Increase in Central Japan in 2001-2002 of Neisseria gonorrhoeae Isolates with Decreased Susceptibility to Penicillin, Tetracycline, Oral Cephalosporins, and Fluoroquinolones

ABSTRACT Four hundred sixty-two clinical isolates of Neisseria gonorrhoeae recovered from 1999 through 2002 in central Japan were examined for MICs of antimicrobial agents. The majority was sensitive to ceftriaxone and spectinomycin, but a remarkable increase in isolates with decreased susceptibility to penicillin, tetracycline, oral cephalosporins, and fluoroquinolones was observed from 2001 through 2002.
Low RPS14 expression is common in myelodysplastic syndromes without 5q- aberration and defines a subgroup of patients with prolonged survival

This paper shows that the expression of the ribosomal protein S14 (RPS14) is reduced in about two thirds of patients with non-5q- myelodysplastic syndrome. See related perspective article on page 1336. To further clarify the role of ribosomal protein S14 (RPS14) in myelodysplastic syndrome, we examined RPS14 transcription in bone marrow derived CD34+ cells from patients with non-5q- myelodysplastic syndrome and found a reduced expression of RPS14 in 51 of 72 (71%) patients. MDS patients with an intermediate-1 risk (INT-1) score according to the international prognostic scoring system and low RPS14 expression had a superior median overall survival of not reached versus 25 months compared to INT-1 patients with high RPS14 expression (p=0.0249). Using multivariate analysis, the RPS14 expression status was confirmed as an independent predictor for survival in INT-1 patients.
Interplay of phenyl-perfluorophenyl stacking, C-H...F, C-F...pi and F...F interactions in some crystalline aromatic azines.

Analysis of phenyl-perfluorophenyl stacking synthon, C-H.F, C-F.pi interactions, and F.F tetramer in three closely related azine crystal structures shows the dominance of Ar-ArF synthon while other interactions are turned on/off depending on the H/F stoichiometry in the molecule.
Periconception maternal smoking and low education are associated with methylation of INSIGF in children at the age of 17 months

Maternal smoking during pregnancy and a low socioeconomic status (SES) lead to increased risks of adverse pregnancy outcome. Maternal education is often used as proxy for SES. We explored the programming of the insulin pathway genes IGF2 DMR (insulin growth factor 2 differentially methylated region), IGF2R (insulin growth factor 2 receptor) and INSIGF [the overlapping region of IGF2 and insulin (INS)] in the child through any periconception maternal smoking and education level. In 120 children at 17 months of age, methylation of DNA derived from white blood cells was measured. Periconception smoking and low education were independently associated with INSIGF methylation and showed a relative increase in methylation of +1.3%; P = 0.043 and +1.6%; P = 0.021. Smoking and low education showed an additive effect on INSIGF methylation (+2.8%; P = 0.011). There were no associations with IGF2 DMR and IGF2R methylation. Our data suggest that periconception maternal smoking and low education are associated with epigenetic marks on INSIGF in the very young child, this warrants further study in additional populations.
Pengembangan Buku Ajar Mata Kuliah Bahasa Indonesia Untuk Mahasiswa STMIK Palangka Raya

Penelitian ini bertujuan mendeskripsikan proses perkuliahan Bahasa Indonesia di STMIK Palangka Raya, mengembangkan buku ajar mata kuliah Bahasa Indonesia yang sesuai dengan capaian kompetensi lulusan STMIK Palangka Raya, dan mengevaluasi validitas, efektivitas, serta kepraktisan buku ajar tersebut. Metode yang digunakan adalah Research and Development dengan menganalisis kebutuhan kemudian mengembangkan produk dan diuji validitas, efektivitas, dan kepraktisanya hingga diperoleh produk final. Produk yang dikembangkan adalah buku ajar mata kuliah Bahasa Indonesia untuk mahasiswa STMIK Palangka Raya. Hasil analisis menunjukkan buku ajar validitas sebesar 93%, efektif kerena sebesar 92,67% mahasiswa tuntas KKM, dan praktis karena mahasiswa sebesar 87,33% merespon keterterapan produk dengan baik, serta 89,60% praktisi merespon dengan baik saat diterapkan. Buku ajar ini memiliki keterterapan yang tinggi karena memiliki validitas yang tinggi pula. Buku ajar yang dikembangkan juga memiliki efektivitas yang baik, karena memiliki validitas yang tinggi dan kepraktisan yang tinggi pula. Dengan kepraktisan buku ajar yang tinggi, maka belajar materi Bahasa Indonesia akan lebih mudah.Disarankan agar dosen dapat menerapkan buku ajar Bahasa Indonesia untuk mahasiswa STMIK Palangka Raya guna membekali mahasiswa untuk cakap menerapkan kaidah-kaidah Bahasa Indonsia yang baik dan benar, dalam bidang ilmu pengetahuan dan teknologi maupun dalam kehidupan sehari-hari.
Measuring affect at work: confirmatory analyses of competing mood structures with conceptual linkage to cortical regulatory systems.

Twenty so-called clear markers of positive and negative mood (Watson & Tellegen, 1985) were applied in the current study to measure affect at work. Confirmatory factor analyses of a bipolar Two-Factor (i.e., positive and negative affect) Model and a competing multifactor model were conducted with three samples: managerial and professional workers in an insurance firm, retail sales personnel, and a heterogeneous group of students who were employed. The first-order Two-Factor Model (i.e., descriptively bipolar positive and negative affect factors) hypothesized to underlie the 20 affect items did not provide a strong fit to the data in the three samples. A first-order Four-Factor Model with descriptively unipolar factors labeled as Positive Arousal (Enthusiasm), Negative Activation (Nervousness), Low Arousal (Fatigue), and Low Activation (Relaxation) provided a better fit across the samples. These results support the measurement of positive and negative mood as descriptively unipolar factors. The measurement implications of these results as well as conceptual linkages between the four mood factors and the two major cortical regulatory systems, left-lateralized dopaminergic activation and right-lateralized noradrenergic arousal, are discussed.
Prognosis of patients with acute renal failure without cardiopathy

BACKGROUND The outcome for children with acute renal failure (ARF) may be poor. However, relatively few published studies have considered prognosis of these patients. METHODS We prospectively studied, from 1978 to 1998, 92 such children without heart disease to try to identify risk factors for mortality. RESULTS Forty five per cent of children with tumours, shock, and other causes died compared with none of those with a primary urinary tract related problem. ARF did not seem to be the cause of death in any case. Univariate analysis showed that in the non-primary urinary problem group (55 cases), patients with hypotension, high values of BUN or creatinine, or who needed mechanical ventilation or dialysis, had a poor outcome. Multivariate analysis showed that probability of death can be estimated using the following score: −0.02 + 0.28 (hypotension) + 0.19 (ventilation) + 0.27 (dialysis) + 0.01 (BUN). CONCLUSIONS Mortality of patients with ARF was related to aetiology, the need for dialysis and/or ventilator use, hypotension, and BUN values.
Versatile Transcription of Biphenyl Catabolic bphOperon in Pseudomonas pseudoalcaligenes KF707*

Pseudomonas pseudoalcaligenes KF707 possesses a chromosomally encoded bph gene cluster responsible for the catabolism of biphenyl/polychlorinated biphenyls. The gene cluster consists of(orf0)bphA1A2(orf3)bphA3A4BCX0X1X2X3D. We studied the role of orf0 and transcription in the KF707bph operon. Primer extension analyses revealed that at least as many as six transcriptional initiation sites exist upstream oforf0, bphA1, bphX0,bphX1, and bphD, including two upstream ofbphD. The orf0-disruptant failed to grow on biphenyl but accumulated large amounts of the biphenyl ringmeta-cleavage yellow compound (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate). Western blot analysis revealed that ORF0 protein is inducibly expressed in KF707 in the presence of biphenyl. Gel shift assay revealed that ORF0 directly binds to the orf0 operator region. This binding was greatly enhanced by addition of the biphenyl ring meta-cleavage yellow compound. These results indicated that orf0,bphA1A2(orf3)bphA3A4BC and bphX0X1X2X3Dare independently transcribed, and that ORF0 protein belonging to the GntR family is involved in the regulation of the bphoperon in KF707 and is absolutely required for the expression oforf0 and bphX0X1X2X3D.
Burden of Neonatal Infections in Developing Countries: A Review of Evidence From Community-Based Studies

Introduction: Infections are a major contributor to newborn deaths in developing countries. Majority of these deaths occur at home without coming to medical attention. The Millennium Development Goal for child survival cannot be achieved without substantial reductions in infection-specific neonatal mortality. We describe the burden of neonatal infections in developing countries and discuss the need for community-based management approaches to improve survival from neonatal infections in these countries. Methods: We reviewed community-based studies published since 1990 from developing countries to estimate the rates of neonatal and young infant infections and infection-specific neonatal mortality. Results: Thirty-two studies reviewed suggest that infections may be responsible for 8% to 80% of all neonatal deaths and as many as 42% of deaths in the first week of life. Eleven reports provided data on incidence of infections in neonates and infants up to 60 days of life. Rates of neonatal sepsis were as high as 170/1000 live births (clinically diagnosed) and 5.5/1000 live births (blood culture-confirmed). Conclusions: Considerable heterogeneity exists among included studies, and more accurate data and standardized methodologies are required. However, data indicate that a significant proportion of neonatal deaths in developing countries are due to infections. Current recommendations of hospitalization and parenteral therapy for managing neonatal infections are inadequately followed in developing countries. Approaches for detecting and managing serious infections within the community, at home or first-level health facilities, may be more effective options in settings where delays and reluctance to seek care, health system inefficiencies, socioeconomic and cultural, as well as logistic constraints exist.
Effects of question type and eyewitness temperament on accuracy and quantity of recall for a simulated misdemeanor crime.

The present study examined the effect of question format on accuracy and quantity of testimony. Forty college students’ memory for a videotaped theft was assessed through open-ended or multiple-choice questionnaires. High accuracy was found for central information elicited with an open-ended questionnaire and for peripheral information elicited with a multiple-choice questionnaire. Quantity was higher for central than for peripheral features and was higher with multiplechoice than with open-ended questionnaires. Lipton’s (1977) notion of an inverse relationship between accuracy and quantity due to cognitive set was modified in order to incorporate the role question format had on different types of information. Several witness temperament characteristics were associated with central and peripheral recall using multiple-choice questionnaires.
Fluid Modeling and Analysis of the Constriction of the DC Positive Column in Argon

The glow-to-arc transition of the positive column of a dc discharge in argon in the course of constriction has been investigated on the basis of a self-consistent 1-D axisymmetric fluid model. The model adopts the nonlocal moment method, i.e., the system of balance equations resulting from the moments of the radially dependent Boltzmann equation is solved. The electron transport and rate coefficients are applied in dependence on the mean energy of the electrons, the gas temperature, and the ionization degree. Investigations have been performed for currents from 0.6 to 70 mA and pressures from 100 to 500 torr. The model predictions are compared with experimental and other available modeling results, and they show good agreement with these data in general. The pronounced nonlocal features of the mean electron energy balance are found, and their influence on the constricted argon positive column is analyzed. Different assumptions concerning the electron velocity distribution function have been considered in the present model. In particular, the impact of using a Maxwellian distribution instead of solutions of the steady-state spatially homogeneous electron Boltzmann equation is discussed where the assumption of a Maxwellian distribution for the electrons was found to be inappropriate for describing the constriction effect.
Cerebellar activation in opsoclonus

It is controversial whether opsoclonus is a cerebellar or brainstem disorder. Two patients whose opsoclonus largely disappeared on eye closure underwent fMRI. A comparison of these two states revealed neither vermal nor brainstem activation but rather a bilateral activation in the deep cerebellar nuclei in excess of what the authors found in healthy subjects. The results support a crucial role of the fastigial nucleus in opsoclonus.