Miracle-dz/UniFAR-FTUs huggingface dataset:
Unilateral uniplanar external skeletal fixation for isolated diaphyseal tibial fractures in skeletally immature dogs.

OBJECTIVE To report and evaluate use of external skeletal fixation (ESF) for repair of isolated (fibula intact) diaphyseal tibia fractures in skeletally immature dogs. STUDY DESIGN Case series. ANIMALS Skeletally immature (mean age, 17 weeks; range, 12-23 weeks) dogs (n=5) with isolated diaphyseal tibial fractures. METHODS Medical records (2006-2007) of 5 dogs with isolated diaphyseal tibial fractures treated with Type 1a ESF were reviewed and outcome assessed by clinical examination and telephone interviews. RESULTS Dogs were evaluated approximately 2 and 4 weeks after surgery. Limb function, muscle mass, and comfort level remained very good to excellent throughout healing. Bony union was confirmed radiographically and the ESF removed approximately 4 weeks after surgery (mean, 31.4 days; range, 28-37 days). Owner satisfaction was high when contacted a minimum of 4 weeks after ESF removal. CONCLUSIONS Use of Type Ia ESF with positive profile threaded pins is a practical and biologic method for treatment of isolated tibial fractures in skeletally immature dogs CLINICAL RELEVANCE ESF is an effective alternative treatment to external coaptation and internal fixation for isolated diaphyseal tibial fractures in skeletally immature dogs.
Up-regulation of Caveolae and Caveolar Constituents in Multidrug-resistant Cancer Cells*

Cancer chemotherapy often results in the development of multidrug resistance (MDR), which is commonly associated with overexpression of P-glycoprotein (P-gp), a plasma membrane drug efflux ATPase. It was shown recently that glycosphingolipids are elevated in MDR cells. Sphingolipids are major constituents of caveolae and of detergent-insoluble, glycosphingolipid-rich membrane domains. Here we report that multidrug-resistant HT-29 human colon adenocarcinoma cells exhibit a 12-fold overexpression of caveolin-1, a 21-kDa coat/adaptor protein of caveolae. Similar observations were made in adriamycin-resistant MCF-7 human breast adenocarcinoma cells. Caveolin-2 expression is also up-regulated in MCF-7-AdrR cells, but neither caveolin-1 nor caveolin-2 were detected in MCF-7 cells stably transfected with P-gp. The up-regulation of caveolins is associated with a 5-fold increase in the number of caveolae-like structures observed in plasma membrane profiles of HT-29-MDR cells and with the appearance of a comparable number of caveolae in MCF-7-AdrR cells. A significant fraction (∼40%) of cellular P-gp is localized in low density detergent-insoluble membrane fractions derived from either HT-29-MDR or MCF-7-AdrR cells. The distribution of recombinant P-gp in stably transfected MCF-7 cells was similar, even though these cells do not express caveolins and are devoid of caveolae. The possibility that caveolae contribute to the multidrug resistance and thus are co-selected with P-gp during the acquisition of this phenotype is discussed.
Chitosan-gelatin sheets as scaffolds for muscle tissue engineering

Abstract Hydrogels made of natural polymers [chitosan (CS) and gelatin (G)] have been prepared having mechanical properties similar to those of muscle tissues. In this study, the effect of polymer concentration and scaffold stiffness on the behavior of seeded muscle-derived cells (MDCs) on the CS-G hydrogel sheets has been evaluated. Both variables were found to be important in cell viability. Viability was assessed by observation of the cell morphology after 1 day as well as a 14-day MTT assay. The CS-G hydrogels were characterized using Fourier transform infrared (FTIR) analysis, which revealed evidences of strong intermolecular interactions between CS and G. Hydrogel samples with intermediate concentration of CS had suitable handling characteristics for surgical purposes as well as similar elasticity to muscle tissues. The sample with intermediate stiffness (22 ± 1kPa) exhibited the greatest attachment, expansion, and proliferation rate. Such CS-G hydrogels with intermediate stiffness may be considered as new candidates for muscle tissue engineering in the reconstructive field of urology.
Intravenous Thrombolysis for Acute Cerebral Ischaemia: Comparison of Outcomes between Patients Treated at Working versus Nonworking Hours

Background: Stroke outcomes are worse in patients admitted at nonworking hours (NWH), but whether this is also true in patients treated with intravenous (i.v.) thrombolysis has not been definitely proven. Objective: Our aim was to test the hypothesis that stroke patients treated by i.v. rt-PA at NWH have a worse outcome than those treated at working hours (WH). Methods: We compared outcomes at 7 days and at 3 months, between patients treated at NWH and at WH in the stroke unit of the Lille University Hospital. Results: Of 252 consecutive patients [median age: 69 years; 132 men (52.4%); median National Institutes of Health Stroke Scale score: 14; median onset-to-needle time: 150 min], 134 (53.2%) were treated at NWH. They did not differ for baseline characteristics and proportion of patients with modified Rankin Scale scores 0–1 and 0–2 at 3 months. Patients treated at WH were more likely to die before 7 days (12.7 vs. 4.5%; adjusted odds ratio: 3.6; 95% confidence interval: 1.2–10.4) and at 3 months (21.6 vs. 11.4%; adjusted odds ratio: 2.2; 95% confidence interval: 1.02–4.7). The causes of death did not differ between NWH and WH. At NWH, there was no difference in baseline characteristics and outcomes of patients treated by stroke- and nonstroke neurologists. Conclusion: The case fatality rates were unexpectedly higher at WH than at NWH. If this finding can be reproduced and is not a chance finding, we should identify explanations, especially organisational issues, chronobiological factors or summation of subtle – nonsignificant – baseline differences.
Impact of resident surgeons on procedure length based on common pediatric otolaryngology cases

Surgical education remains an important mission of academic medical centers. Financial pressures may favor improved operating room (OR) efficiency at the expense of teaching in the OR. We aim to evaluate factors, such as resident participation, associated with duration of total OR, as well as procedural time of common pediatric otolaryngologic cases.
Gun policy and serious mental illness: priorities for future research and policy.

OBJECTIVE In response to recent mass shootings, policy makers have proposed multiple policies to prevent persons with serious mental illness from having guns. The political debate about these proposals is often uninformed by research. To address this gap, this review article summarizes the research related to gun restriction policies that focus on serious mental illness. METHODS Gun restriction policies were identified by researching the THOMAS legislative database, state legislative databases, prior review articles, and the news media. PubMed, PsycINFO, and Web of Science databases were searched for publications between 1970 and 2013 that addressed the relationship between serious mental illness and violence, the effectiveness of gun policies focused on serious mental illness, the potential for such policies to exacerbate negative public attitudes, and the potential for gun restriction policies to deter mental health treatment seeking. RESULTS Limited research suggests that federal law restricting gun possession by persons with serious mental illness may prevent gun violence from this population. Promotion of policies to prevent persons with serious mental illness from having guns does not seem to exacerbate negative public attitudes toward this group. Little is known about how restricting gun possession among persons with serious mental illness affects suicide risk or mental health treatment seeking. CONCLUSIONS Future studies should examine how gun restriction policies for serious mental illness affect suicide, how such policies are implemented by states, how persons with serious mental illness perceive policies that restrict their possession of guns, and how gun restriction policies influence mental health treatment seeking among persons with serious mental illness.
Cardiac Rupture After Myocardial Infarction: New Insights From Murine Models

Cardiac rupture after a myocardial infarction is an uncommon event with devastating consequences. Although the clinical features of rupture have been described, the genetic and molecular influences on this outcome in patients are less certain. In mice, at least 17 genetic models have been developed that enhance or suppress the likelihood of rupture postmyocardial infarction. The purpose of this review is to describe these recent advances, recognizing that nearly all of the information has been obtained from mouse models of free wall rupture. Although it is probable that the same genetic determinants apply to septal and papillary muscle rupture, the possibility remains that there are unique modulators of risk for rupture at differing anatomic sites within the heart. It is likely that the candidate genes also influence rupture in humans, although this conclusion must be confirmed. The mouse models will be helpful to direct future proteomic and genomic studies in patients and may already suggest certain fundamental pathways. For example, the essential role of collagen production and stabilization postmyocardial infarction may direct therapies to enhance collagen cross-linking and limit its degradation as a strategy to reduce rates of rupture and enhance myocardial healing.
Visual Fields in Young Children Treated with Vigabatrin

Purpose. To evaluate white sphere kinetic perimetry (WSKP) as a test of the peripheral visual field in young children with a history of epilepsy and treatment with Vigabatrin (VGB). VGB is an antiepileptic medication that is associated with visual field constriction. Methods. Thirty-one VGB patients and 10 control subjects, median age 6 years, were recruited. Visual field extent on the major oblique meridia was tested with a 6° white sphere and WSKP, a method used by Quinn et al. to study field extent in children with retinopathy of prematurity. The same meridia were tested using Goldmann kinetic perimetry (GKP; 1.7° target) in those who were capable. Monocular and binocular tests were conducted. Visual field extent for WSKP and GKP were compared in VGB patients and control subjects. Results. Twenty-eight of 31 VGB patients were testable with binocular WSKP and their median visual field extents were smaller than controls. In 8 of 28 (29%) VGB patients, binocular field extents were smaller than the minimum in the control subjects. Monocular WSKP results did not differ between VGB patients and control subjects. Nine VGB patients were testable with both WSKP and GKP; visual field extents did not differ between tests. Conclusions. WSKP is feasible in VGB patients, even in those with developmental delays. WSKP has the potential to detect visual field constriction associated with VGB use.
Contribution of mast cells to injury mechanisms in a mouse model of pediatric traumatic brain injury

The cognitive and behavioral deficits caused by traumatic brain injury (TBI) to the immature brain are more severe and persistent than injuries to the adult brain. Understanding this developmental sensitivity is critical because children under 4 years of age of sustain TBI more frequently than any other age group. One of the first events after TBI is the infiltration and degranulation of mast cells (MCs) in the brain, releasing a range of immunomodulatory substances; inhibition of these cells is neuroprotective in other types of neonatal brain injury. This study investigates for the first time the role of MCs in mediating injury in a P7 mouse model of pediatric contusion‐induced TBI. We show that various neural cell types express histamine receptors and that histamine exacerbates excitotoxic cell death in primary cultured neurons. Cromoglycate, an inhibitor of MC degranulation, altered the inflammatory phenotype of microglia activated by TBI, reversing several changes but accentuating others, when administered before TBI. However, without regard to the time of cromoglycate administration, inhibiting MC degranulation did not affect cell loss, as evaluated by ventricular dilatation or cleaved caspase‐3 labeling, or the density of activated microglia, neurons, or myelin. In double‐heterozygous cKit mutant mice lacking MCs, this overall lack of effect was confirmed. These results suggest that the role of MCs in this model of pediatric TBI is restricted to subtle effects and that they are unlikely to be viable neurotherapeutic targets. © 2016 Wiley Periodicals, Inc.
Four new cases of stomatin‐deficient hereditary stomatocytosis syndrome: association of the stomatin‐deficient cryohydrocytosis variant with neurological dysfunction

This report concerns congenitally Na+–K+ leaky red cells of the ‘hereditary stomatocytosis’ class. Three new isolated cases and one new pedigree are described, and one previously reported case is expanded. In all cases, Western blotting of red cell membranes revealed a deficiency in the 32 kDa membrane protein, stomatin. All showed pronounced cation leaks at 37°C with markedly abnormal intracellular Na+ and K+ concentrations, like all other such stomatin‐deficient cases. Consistent with recent findings in two previously described British pedigrees, immunocytochemistry demonstrated that the deficiency of stomatin was not complete. On typical blood films, some red cells showed positive stomatin immunoreactivity, while most were negative, although in one case only a minority were negative. All platelets and neutrophils were stomatin positive. The cases differed markedly between themselves with regard to the temperature dependence of the passive leak to K+. Three showed a simple monotonic temperature dependence, while two showed a minimum at around 20–25°C, such that the cells were extremely leaky at 0°C, giving the phenotype known as ‘cryohydrocytosis’. These patients are the only two known cases of stomatin‐deficient cryohydrocytosis. Both showed a congenital syndrome of mental retardation, seizures, cataracts and massive hepatosplenomegaly, probably defining a new haemato‐neurological syndrome.
Memory Address Scrambling Revealed Using Fault Attacks

Today’s trend in the smart card industry is to move from ROM+EEPROM chips to Flash-only products. Recent publications have illustrated the vulnerability of Floating Gate memories to UV and heat radiation. In this paper, we explain how, by using low cost means, such a vulnerability can be used to modify specific data within an EEPROM memory even in the presence of a given type of counter-measure. Using simple means, we devise a fault injection tool that consistently causes predictable modifications of the targeted memories’ contents by flipping ‘1’s to ‘0’s. By mastering the location of those modifications, we illustrate how we can reverse-engineer a simple address scrambling mechanism in a white box analysis of a given EEPROM. Such an approach can be used to test the security of Floating Gate memories used in security devices like smart cards. We also explain how to prevent such attacks and we propose some counter-measures that can be either implemented on the hardware level by chip designers or on the software level in the Operating System interacting with those memories.
Neuroanatomical Abnormalities in Behaviorally Characterized APPV717F Transgenic Mice

Histological analyses were performed on the brains of APP(V717F) transgenic (Tg)mice previously studied in a battery of behavioral tests. We describe here the regional and age-dependent deposition of amyloid in both heterozygous and homozygous Tg mice. We also report that Tg mice show significant and age-dependent changes in synaptic density measured by synaptophysin immunoreactivity. Surprisingly, a rather marked hippocampal atrophy is observed as early as 3 months of age in Tg mice (20-40%). Statistical analyses revealed that the deficits in object recognition memory are related to the number of amyloid deposits in specific brain regions, whereas deficits in spatial reference and working memory are related to the changes in synaptic density and hippocampal atrophy. Our study suggests that the behavioral deficits observed in Tg mice are only in part related to amyloid deposition, but are also related to neuroanatomical alterations secondary to overexpression of the APP(V717F) transgene and independent of amyloid deposition.
Lessons From a Comparison of Work Domain Models: Representational Choices and Their Implications

As methods in cognitive work analysis become more widely applied, questions regarding the impact of modeling choices and similarities in modeling efforts across projects and domains are increasingly relevant. However, no explicit comparison of models of similar systems has been reported. This paper compares independently developed work domain analysis (WDA) models of two command and control environments. Similarities in model content and the types of nodes included provide evidence that WDA techniques can capture fundamental elements regarding purposes and constraints. These points of agreement provide a common starting point for developing work domain representations of military command and control systems. The comparison also revealed differences between the models. Although differences in content reflected differences in scope of coverage and level of detail, other differences corresponded to more fundamental choices in modeling approach. These included the treatment of sensors, level of integration in the model, and representation of particular abstract constraints. Examination of these more fundamental differences pointed to important degrees of freedom in how to represent a WDA and clarified the implications of these modeling choices for guiding design. Actual or potential applications of this research include aiding analysts in making work domain modeling choices as well as producing work domain models of command and control environments.
Structural joint changes, malalignment, and laxity in osteoarthritis of the knee

Objective: To assess the relationship between (i) structural joint changes (i.e. joint space narrowing and osteophyte formation) and laxity and (ii) joint malalignment and laxity in osteoarthritis (OA) of the knee. Methods: A cross‐sectional study was carried out on 35 outpatients with osteoarthritis of the knee. Weight‐bearing radiographs of the knees were used to assess joint space narrowing (JSN) and osteophyte formation. Knee joint laxity was assessed using a device that measures the angular deviation of the knee in the frontal plane (varus–valgus laxity). Malalignment was assessed using a goniometer. All analyses were performed using knees as units of analysis (i.e. 70 knees). Results: The mean laxity of 70 knees was 8.0±4.1°. Knees with minute JSN were significantly more lax than knees with no JSN. There was no significant relationship between osteophyte formation and laxity. Malaligned knees were significantly more lax than aligned knees. Conclusion: Both joint space narrowing and malalignment are related to laxity. These results support the premise that biomechanical factors play a role in the degeneration of the osteoarthritic knee joint.
Thermal unfolding of staphylococcal nuclease and several mutant forms thereof studied by differential scanning calorimetry

The effects of eight mutations on the thermodynamics of the reversible thermal unfolding of staphylococcal nuclease have been determined over a range of pH and protein concentration by means of differential scanning calorimetry. Variation of the protein concentration was included in our study because we found a significant dependence of the thermodynamics of protein unfolding on concentration. Values for the change in the standard free energy of unfolding, ΔΔGd0, produced by the mutations in the pH range 5.0–7.0 varied from 1.9 kcal mol−1 (apparent stabilization) for H124L to ‐2.8 kcal mol−1 (apparent destabilization) for L25A. As has been observed in numerous other cases, there is no correlation in magnitude or sign between ΔΔGd0 and the corresponding values for ΔΔHd and TΔΔSd0, the latter quantities being in most cases much larger in magnitude than ΔΔGd0. This fact emphasizes the difficulty in attempting to correlate the thermodynamic changes with structural changes observed by X‐ray crystallography.
Biased declarative questions in Swedish and German

This paper investigates a class of biased questions with declarative syntax in Swedish and German that differ in their bias from the familiar class of declarative questions: rejecting questions (RQs), which may occur with or without negation. We provide a semantic-pragmatic analysis of RQs and show for negative RQs that the negation is non-propositional. We analyze the non-propositional negation as the speech-act modifying operator FALSUM (Repp 2009a, 2013). In both languages, FALSUM interacts with modal particles whose meanings relate to contrast and the epistemic state of the speaker. We propose that the illocutionary operator in RQs is REJECTQ, which is an operator that comes with presuppositions that are the source of the particular bias of RQs.
Nutrition, labour productivity and food security in Thailand

Many households in less developed countries suffer from food insecurity which is unreliable access to a sufficient quantity of nutritious food. It is a major cause of malnutrition, and may lead to reduced worker capacity and low productivity. This study examines the impact of nutrient intake on the productivity of rice-producing households in Thailand. There are three objectives: first, to analyse the relationship between nutrient intake and labour productivity; second, to examine factors affecting the nutrition-labour productivity relationship; and third, to study the links between nutrition, labour productivity and food security. Agricultural household models are used to examine decision-making behaviour, namely production, consumption, and labour allocation. The efficiency wage hypothesis is also examined where an increase in nutrient consumption increases labour productivity. Accordingly, labour is determined by caloric consumption, and nutrition affects productivity. The empirical study adopts econometric methods with data from Thailand's Socio-Economic Survey for 2011 for 2,781 rice-farming households. A semi-log wage equation and a CobbDouglas production function are estimated; and a logit model is used to examine the determinants of food security on the production-consumption relationship. Results from the wage equation show that increasing consumption of calcium, vitamin A, vitamin C and iron increase household income, while increasing calorie intake reduces income. An increase in the consumption of grains and starches reduces income, whereas extra consumption of meat and poultry, fruits, vegetables and nuts lead to an increase in income. Male household heads earn more than female heads. Higher levels of education, age, the dependency ratio, and farm size increase income. In the production function, all nutrients affect farm productivity positively which supports the efficiency wage hypothesis. The logit results show that income, education, food expenditure, owning livestock, production for ownconsumption, farm size, fertiliser use, and the use of family labour improve food-security; while household size, the dependency ratio, and total household expenditure do not. In conclusion, enhancing micronutrient intake is an investment for improving productivity. The Thai government should focus on building awareness of nutrition in diet and provide dietary guidelines. Food quality and safety standards should be promoted to improve accessibility to nutritious foods. Policies on vitamin and mineral fortification of processed foods, including cooking oils, flours, salt, and sweetness additives, could be designed to improve nutrientcontent.