Miracle-dz/UniFAR-FTUs huggingface dataset:
Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma

The formation of distant metastases often determines the fate of patients with head and neck squamous cell carcinoma (HNSCC). The expression of cell adhesion molecules (CAMs) and their ligands of the leukocyte adhesion cascade has been associated with metastatic competence in several malignant entities. In this study, human HNSCC cell lines were analyzed in vitro and in a spontaneous metastatic xenograft model. Immunohistochemical analyses of several CAMs were performed on xenograft tumors and tissue microarrays (TMA) from 453 patients with head and neck squamous cell carcinomas with full histo-pathological and clinical follow-up data. UTSCC 24A and 24B cells bind to E-selectin in vitro, show E-selectin dependent binding to human umbilical vein endothelial cells (HUVECs), and express sLeX. All HNSCC cells engrafted into severe combined immunodeficient (SCID) mice, and UTSCC 24A cells formed sporadically spontaneous lung metastases. The expression of CAMs varied between the cell lines, but a correlation between tumor growth and metastatic potential did not exist. None of the CAMS or their ligands could be identified to be of prognostic relevance in the TMA study. The in vitro results indicate that E-selectin and sLeX are involved in the adhesion of HNSCC cells to endothelium. However, specific prognostic markers chosen from the leukocyte adhesion cascade for HNSCC were not identified.
Impact of the parent‐child relationship on psychological and social resilience in pediatric cancer patients

The primary objective of this research was to examine patterns of parent‐child relationship functioning among pediatric cancer survivors and their caregivers across a variety of relationship indicators (ie, Involvement, Attachment, Communication, Parenting Confidence, and Relational Frustration), and evaluate how these factors relate to psychosocial outcomes in survivors.
[Self-reported hearing loss and visual impairment in adults from Central Mexico].

OBJECTIVE To determinate the prevalence of hearing loss (HL) and visual impairment (VI) among adult population from Tlaxcala, Mexico. MATERIALS AND METHODS A populationbased cross-sectional study comprising persons 50 years and older was conducted in 2013. Self-reported HL was measured using the Hearing Impairment Inventory for the Elderly (SHIIE) questionnaire; VI was determined using the Snellen tumbling E chart. RESULTS 900 women and 611 men (mean age 66.1 years) were included. 481(31.8%) individuals had HL (415 alone and 66 combined with VI). Prevalence of HL alone and together with VI was associated with age (per two years, OR=1.03 and OR=1.18, respectively) and self-reported poor health status (OR=1.90 and OR=3.69, respectively). CONCLUSIONS The high prevalence of these disabilities calls for the implementation of public health interventions that help to reduce its impact in the population.
Ribosome recycling in mRNA translation, quality control, and homeostasis

Abstract Protein biosynthesis is a conserved process, essential for life. Ongoing research for four decades has revealed the structural basis and mechanistic details of most protein biosynthesis steps. Numerous pathways and their regulation have recently been added to the translation system describing protein quality control and messenger ribonucleic acid (mRNA) surveillance, ribosome-associated protein folding and post-translational modification as well as human disorders associated with mRNA and ribosome homeostasis. Thus, translation constitutes a key regulatory process placing the ribosome as a central hub at the crossover of numerous cellular pathways. Here, we describe the role of ribosome recycling by ATP-binding cassette sub-family E member 1 (ABCE1) as a crucial regulatory step controlling the biogenesis of functional proteins and the degradation of aberrant nascent chains in quality control processes.
Nerve lengthening and subsequent end‐to‐end repair yield more favourable outcomes compared with autograft repair of rat sciatic nerve defects

Outcomes of end‐to‐end nerve repairs are more successful compared with outcomes of repairs bridged by nerve grafts. However, end‐to‐end repairs are not always possible for large nerve gaps, as excessive tension may cause catastrophic failure. In this study, we built on previous nerve‐lengthening studies to test the hypotheses that gradual lengthening of the proximal stump across a large nerve gap enables an end‐to‐end repair and such a repair results in more favourable regenerative outcomes than autografts, which represent the gold standard in bridging nerve gaps. To test these, we compared structural and functional outcomes in Lewis rats after repair of sciatic nerve gaps using either autografts or a novel compact internal fixator device, which was used to lengthen proximal nerve stumps towards the distal stump over 2 weeks, prior to end‐to‐end repair. Twelve weeks after the initial injury, outcomes following nerve lengthening/end‐to‐end repair were either comparable or superior in every measure compared with repair by autografting. The sciatic functional index was not significantly different between groups at 12 weeks. However, we observed a reduced rate of contracture and corresponding significant increase in paw length in the lengthening group. This functional improvement was consistent with structural regeneration; axonal growth distal to the injury was denser and more evenly distributed compared with the autograft group, suggesting substantial regeneration into both tibial and peroneal branches of the sciatic nerve. Our findings show that end‐to‐end repairs following nerve lengthening are possible for large gaps and that this strategy may be superior to graft‐based repairs.
Perceptual and Visuo-Motor Disorders in Cerebral Palsy

The author's assignment in this monograph, as outlined in the preface, is "to find out exactly what is or is not known" in the field of concern; and as such it is a task as nobly executed as conceived. Mrs. Abercrombie has provided a concise distillation, with results in round numbers, of more than sixty of the most significant recent studies in this and related fields, together with intelligent commentary and criticism; and has summarized the conclusions of nearly a hundred others.
Plasma Ceramide Species Are Associated with Diabetes Risk in Participants of the Strong Heart Study.

BACKGROUND Few studies have assessed the associations of ceramides and sphingomyelins (SMs) with diabetes in humans. OBJECTIVE We assessed associations of 15 circulating ceramides and SM species with incident diabetes in 2 studies. METHODS The analysis included 435 American-Indian participants from the Strong Heart Study (nested case-control design for analyses; mean age: 57 y; 34% male; median time until diabetes 4.3 y for cases) and 1902 participants from the Strong Heart Family Study (prospective design for analyses; mean age: 37 y; 39% male; median 12.5 y of follow-up). Sphingolipid species were measured using stored plasma samples by sequential LC and MS. Using logistic regression and parametric survival models within studies, and an inverse-variance-weighted meta-analysis across studies, we examined associations of 15 ceramides and SM species with incident diabetes. RESULTS There were 446 cases of incident diabetes across the studies. Higher circulating concentrations of ceramides containing stearic acid (Cer-18), arachidic acid (Cer-20), and behenic acid (Cer-22) were each associated with a higher risk of diabetes. The RRs for incident diabetes per 1 SD of each log ceramide species (μM) were 1.22 (95% CI: 1.09, 1.37) for Cer-18, 1.18 (95% CI: 1.06, 1.31) for Cer-20, and 1.20 (95% CI: 1.08, 1.32) for Cer-22. Although the magnitude of the risk estimates for the association of ceramides containing lignoceric acid (Cer-24) with diabetes was similar to those for Cer-18, Cer-20, and Cer-22 (RR = 1.13; 95% CI: 1.01, 1.26), the association was not statistically significant after correction for multiple testing (P = 0.007). Ceramides carrying palmitic acid (Cer-16), SMs, glucosyl-ceramides, or a lactosyl-ceramide were not associated with diabetes risk. CONCLUSIONS Higher concentrations of circulating Cer-18, Cer-20, and Cer-22 were associated with a higher risk of developing diabetes in 2 studies of American-Indian adults. This trial was registered at clinicaltrials.gov as NCT00005134.
The impact of varying interphase interval on neuromuscular electrical stimulation-induced quadriceps femoris muscle performance and perceived discomfort

ABSTRACT Objectives: Determine the effects of varying frequency, phase duration, and interphase interval (IPI) of symmetrical, biphasic pulsed current (SBPC) on quadriceps femoris muscle torque production, perceived discomfort, and muscle fatigue when using neuromuscular electrical stimulation (NMES). Methods: Ten recreationally active able-bodied participants completed this study. Muscle torque was measured during a series of laboratory based, NMES-induced muscle contractions with varying combinations of phase durations (50, 100, 150, or 200 µs) and IPIs (50, 100, 150, or 200 µs) at 25 and 50 Hz. A three-factorial repeated measures experimental design was used. After each contraction, participants rated perceived discomfort on a 10-cm visual analog scale. Subsequently, fatigue tests were conducted using different frequency-IPI combinations. Results: Results of within-subjects ANOVAs revealed no interaction among the three factors: frequency, phase duration and IPI. Significant main effects of phase duration and IPI, but not frequency, on torque production and perceived discomfort were observed. Specifically, significant linear and quadratic trends were observed for the effect of phase duration and IPI on torque production, and the effect of IPI on discomfort, and only a significant linear trend for the effect of phase duration on discomfort. The shorter IPI combined with the longer phase duration produced greater torque with tolerable discomfort. There was no significant effect of IPI on muscle fatigue. Conclusions: Study findings indicated that when administering SBPC on the quadriceps femoris muscle, clinicians should select an IPI of 50 µs and a longer phase duration (e.g. 200 µs) to maximize torque without imposing intolerable discomfort.
Physicians' attitudes towards accelerated access to medicines

Abstract In recent years, a variety of ‘accelerated access’ schemes have been introduced by pharmaceutical regulators and funders globally. These schemes aim to overcome perceived regulatory and reimbursement barriers to accessing medicines – particularly for patients with limited time or therapeutic options. However, patient access to approved medicines is mediated by a number of third parties including regulators and payers, and physicians who act both as gatekeepers and guides to prescribed medications. It is therefore essential to know how physicians think about accelerated access as they are responsible for advising patients on and prescribing medicines made available via these pathways. We conducted semi-structured interviews with 18 Australian physicians focusing on their attitudes towards accelerated access. We identified three ‘archetypes’ of physicians: ‘confident accelerators’, ‘cautious accelerators’, and ‘decelerators’. Although all acknowledged the potential risks and benefits of accelerated access, they disagreed on their magnitude and extent and how they should be balanced in both policy formation and clinical practice. Overall, our results illustrate the diversity of clinical opinions in this area and the importance of monitoring both the prescribing and clinical outcomes that result from accelerated access programmes to ensure that these are both clinically and morally acceptable.
Quantitative assessment of the effect of fasting on macular microcirculation: an optical coherence tomography angiography study

Aim To analyse the effect of fasting on the retinal vascular plexus and choriocapillaris structures in healthy subjects via Optical Coherence Tomography Angiography (OCTA). Methods In this prospective study, OCTA scans of 98 eyes in 49 healthy subjects were compared in fasting and non-fasting period. All cases had a full ophthalmological examination, with OCTA parameters measured, including superficial flow area (SCP) (mm2), deep flow area (DCP) (mm2), choriocapillaris flow area (CC) (mm2), superficial and deep vascular density (SVD and DVD, respectively) (%), superficial foveal avascular zone area (FAZs) (mm2), deep foveal avascular zone area (FAZd) (mm2), subfoveal choroidal thickness (SFCT) (µm) and central macular thickness (CMT) (µm). Results The mean SCP, DCP and CC flow area values were induced to be significantly lower in a fasting than a non-fasting period (p<0.001, p<0.001, p=0.049, respectively). The mean VD in a fasting period was statistically significantly lower than in a non-fasting period at the SCP level (p=0.009). No statistically significant difference was found between the two periods in respect to DVD, FAZd and CMT (p=0.068, p=0.653 and p=0.531, respectively); however, FAZs were significantly lower in a non-fasting than fasting period (p=0.038). The SFCT was determined to be statistically significant and thicker during a fasting period (p<0.001). Conclusion The current study documented several physiological changes in a fasting period, such as changes in SCP, DCP, CC flow, SVD and DVD, via OCTA.
The Rivermead Post-Concussion Questionnaire score is associated with disability and self-reported recovery six months after mild traumatic brain injury in older adults

ABSTRACT Background/Objectives: Post-concussion syndrome refers to the adverse group of symptoms following a mild traumatic brain injury (mTBI). The Rivermead post-concussion syndrome questionnaire (RPQ) is a common clinical tool for assessing baseline post-concussion syndrome symptomology; however, it is unknown if scores on this questionnaire are associated with future disability. Therefore, the goal of this study was to determine the association between baseline RPQ scores and future disability in older adults with mTBI. Methods and Findings: This study used a prospective cohort design, using the RPQ to measure baseline post-concussion syndrome symptomatology. Disability at 6 months was measured using the Glasgow Outcome Scale-Extended (GOSE; disability), short-form 12 (SF-12; physical and mental quality of life), and self-reported recovery. Linear and logistic models adjusted for confounding factors were estimated using 200 bootstrapped samples. Individuals with higher levels of baseline symptomatology were more likely to have poor GOSE scores (RR = 2.13, 95% CI [1.51, 2.31]) and self-reported recovery (RR = 2.64, 95% CI [1.31, 8.98]) 6 months later. Conclusions: High levels of baseline symptomatology may be associated with overall disability and individual perceptions of recovery 6 months post-MTBI. While the RPQ is valid in assessing a patient’s post-concussive symptoms following mTBI, it may not predict long-term physical or mental health in older adults.
TLR7 and TLR8 activate distinct pathways in monocytes during RNA virus infection

Differences in TLR7 and TLR8 signaling program virus-specific responses of human monocytes to infection. TLRing the antiviral response Monocytes are critical orchestrators of immunity that both sense pathogens and produce cytokines. When comparing a range of RNA viruses, de Marcken et al. found that the human monocyte response to infection was virus specific and influenced by Toll-like receptor 7 (TLR7) and TLR8, which sense single-stranded RNA. Whereas TLR8 signaling stimulated monocytes to express type I interferon and cytokines involved in CD4+ T helper 1 (TH1) cell differentiation, TLR7 signaling promoted production of cytokines involved in TH17 cell differentiation. Only TLR7 activated monocyte Ca2+ flux, which suppressed antiviral interferon production. Thus, these receptors activate distinct pathways in monocytes, which can tailor the immune response to viral infection. Human blood CD14+ monocytes are bone marrow–derived white blood cells that sense and respond to pathogens. Although innate immune activation by RNA viruses preferentially occurs through intracellular RIG-I–like receptors, other nucleic acid recognition receptors, such as Toll-like receptors (TLRs), play a role in finely programming the final outcome of virus infection. Here, we dissected how human monocytes respond to infection with either Coxsackie (CV), encephalomyocarditis (EMCV), influenza A (IAV), measles (MV), Sendai (SV), or vesicular stomatitis (VSV) virus. We found that in monocytes, type I interferon (IFN) and cytokine responses to infection were RNA virus specific and differentially involved TLR7 and TLR8, which sense single-stranded RNA. These TLRs activated distinct signaling cascades in monocytes, which correlated with differences in the production of cytokines involved in the polarization of CD4+ T helper cells. Furthermore, we found that TLR7 signaling specifically increased expression of the transcription factor FOSL1, which reduced IL-27 and TNFα production by monocytes. TLR7, but not TLR8, activation of monocytes also stimulated Ca2+ flux that prevented type I IFN responses. Our work demonstrates that in human monocytes, TLR7 and TLR8 triggered different signaling pathways that contribute to distinct phenotypes during RNA virus infection. In addition, we defined individual targets within these pathways that promoted specific T helper and antiviral responses.
Optimisation of methadone treatment in a group of patients on a mephedrone binge and dependent on many psychoactive substances

Abstract Objectives: In recent years, an increase in the frequency of hospitalisations of patients on a mephedrone binge has been observed. The literature lacks data on the optimisation of methadone treatment in this group of people. Methods: The study included 601 patients who took mephedrone on a regular basis between 2010 and 2018. Based on the pharmacological database created, it was verified which methadone interaction contributed to subsequent hospitalisations in the group of people studied and which of them had the best therapeutic effect. Results: During the study, 62.4% of patients received methadone (p < .001). The higher the number of drugs taken together with methadone, the higher the frequency of hospitalisations (p < .001). The highest frequency of re-hospitalisations was recorded in patients who combined mephedrone with at least two other psychoactive substances, as well as those who used methadone with chlorprothixene (p < .001). The most optimal therapeutic effect is characteristic for the intake of methadone with thiazolidine carboxylic acid, namely 95% of people using this type of treatment were hospitalised once (p < .001). Conclusions: Therapy with methadone and thiazolidine carboxylic acid seems to be the most optimal therapy for patients taking mephedrone. Key points The number of hospitalisations of patients receiving mephedrone on a regular basis grows from year to year. The multiple use of poly-pharmacotherapy increased in a group of patients on a mephedrone binge. There is a statistically significant correlation between the number of hospitalisations of patients on a mephedrone binge and the total number of drugs taken together with methadone. Administration of methadone with thiazolidine carboxylic acid was the most effective therapy for patients regularly combining mephedrone with at least two other psychoactive substances.
Technical Note: Assessment of electromagnetic tracking systems in a surgical environment using ultrasonography and ureteroscopy instruments for percutaneous renal access.

PURPOSE Electromagnetic tracking systems (EMTSs) have been proposed to assist the percutaneous renal access (PRA) during minimally invasive interventions to the renal system. However, the influence of other surgical instruments widely used during PRA (like ureteroscopy and ultrasound equipment) in the EMTS performance is not completely known. This work performs this assessment for two EMTSs (Aurora® Planar Field Generator - PFG; Aurora® Tabletop Field Generator - TTFG). METHODS An assessment platform, composed by a scaffold with specific supports to attach the surgical instruments and a plate phantom with multiple levels to precisely translate or rotate the surgical instruments, was developed. The median accuracy and precision in terms of position and orientation were estimated for the PFG and TTFG in a surgical environment using this platform. Then, the influence of different surgical instruments (alone or together), namely analogic flexible ureterorenoscope (AUR), digital flexible ureterorenoscope (DUR), 2D ultrasound (US) probe and 4D mechanical US probe, was assessed for both EMTSs by coupling the instruments to 5-DOF and 6-DOF sensors. RESULTS Overall, the median positional and orientation accuracies in the surgical environment were 0.85 mm and 0.42 º for PFG, and 0.72 mm and 0.39 º for TTFG, while precisions were 0.10 mm and 0.03 º for PFG, and 0.20 mm and 0.12 º for TTFG, respectively. No significant differences were found for accuracy between EMTSs. However, PFG showed a tendency for higher precision than TTFG. AUR, DUR, and 2D US probe did not influence the accuracy and precision of both EMTSs. In opposition, the 4D probe distorted the signal near the attached sensor, making readings unreliable. CONCLUSION Ureteroscopy and ultrasonography-assisted PRA based on EMTS guidance are feasible with the tested AUR or DUR together with the 2D probe. More studies must be performed to evaluate the probes and ureterorenoscopes' influence before their use in PRA based on EMTS guidance.
PENGARUH KEMAMPUAN DAN MOTIVASI KERJA DOSEN TERHADAP KUALITAS LAYANAN KEPADA MAHASISWA

Universitas Kanjuruan Malang E-mail : ian_priyanto@yahoo.com ABSTRACT Reffering to the demands of public service quality, the higher level education as one of public service institutions is hard facing their challenges. Its challenges is a competitiveness. So, It is needed the service quality to the student for facing its competitiveness. This research is conducted to know the influential of Lecturers' competencies and job motivation to the students' services. Analyses method uses regression analyses. Data collection uses questionaires which is given to 64 lecturers as the respondents. They are chossen from 132 lecturers that is relevant as random sampling. The result of research shows that (1) lecturers' competencies has significant influence to the service quality for students, (2) teaching motivations is also significant influencial to the service quality for students, (3) lecturers' competencies has relationship to the motivation for teaching and (4) lecturer's competencie and motivations simultaneously significant influence to the servive quality for the students. Keywords: competency, motivations, service quality, lecturers