Miracle-dz/UniFAR-FTUs huggingface dataset:
Hypothalamic paraventricular nucleus: A quantitative analysis of cytoarchitectonic subdivisions in the rat

The boundaries of subnuclei of the hypothalamic paraventricular nucleus (PVN) were outlined on the basis of a step by step computer‐assisted image analysis of coronal, serial sections of the hypothalamus in rats. The cell size‐frequency histogram, cell packing density, and the cell number were determined for each PVN subdivision. Three principal cell types were distinguished: small, short diameter (d) = 6–10 μm; medium‐sized, d = 10–13 μm; and large, d = 13–19 μm. None of these cell types occur exclusively in any PVN subdivision. According to the predominant cell type, the PVN can be divided into magnocellular, mediocellular and parvicellular subnuclei. Large cells appear to form one subdivision in which the cell packing density is relatively stable. Although the cell sizes showed a relatively large variation, no definitive subgroups (parts) could be distinguished. On the basis of cell packing density, the parvicellular and mediocellular subnuclei can be further divided. The mediocellular cell group can be divided into a dorsal and a posterior subdivision. The parvicellular zone is subdivided into a periventricular and a large medial subdivision. Based on inhomogeneities in cell packing density, the medial subdivision can be further divided into anterior, dorso‐lateral, ventro‐medial and caudal parts.
Burkholderia thailandensis E125 Harbors a Temperate Bacteriophage Specific for Burkholderia mallei

ABSTRACT Burkholderia thailandensis is a nonpathogenic gram-negative bacillus that is closely related to Burkholderia mallei and Burkholderia pseudomallei. We found that B. thailandensis E125 spontaneously produced a bacteriophage, termed φE125, which formed turbid plaques in top agar containing B. mallei ATCC 23344. We examined the host range of φE125 and found that it formed plaques on B. mallei but not on any other bacterial species tested, including B. thailandensis and B. pseudomallei. Examination of the bacteriophage by transmission electron microscopy revealed an isometric head and a long noncontractile tail. B. mallei NCTC 120 and B. mallei DB110795 were resistant to infection with φE125 and did not produce lipopolysaccharide (LPS) O antigen due to IS407A insertions in wbiE and wbiG, respectively. wbiE was provided in trans on a broad-host-range plasmid to B. mallei NCTC 120, and it restored LPS O-antigen production and susceptibility to φE125. The 53,373-bp φE125 genome contained 70 genes, an IS3 family insertion sequence (ISBt3), and an attachment site (attP) encompassing the 3′ end of a proline tRNA (UGG) gene. While the overall genetic organization of the φE125 genome was similar to λ-like bacteriophages and prophages, it also possessed a novel cluster of putative replication and lysogeny genes. The φE125 genome encoded an adenine and a cytosine methyltransferase, and purified bacteriophage DNA contained both N6-methyladenine and N4-methylcytosine. The results presented here demonstrate that φE125 is a new member of the λ supergroup of Siphoviridae that may be useful as a diagnostic tool for B. mallei.
Router for Power Packet Distribution Network: Design and Experimental Verification

A power packet dispatching system is expected to be one of the advanced power distribution systems for controlling electric power, providing energy on demand, and reducing wasted energy consumption. In this paper, power packet routers are designed and experimentally verified for realizing a networked power packet distribution system. While the previously developed router directly forwards the power packet to a load, the new router forwards the packet to the other router with an information tag reattached to the power payload. In addition, the new router can adjust the starting time for forwarding the received power packet to the other site, thus utilizing storage capacity integrated into the router. The results successfully clarify the feasibility of the power packet distribution network.
Neonatal nurse practitioners provide quality, cost-effective care.

Loretta Ford, the pioneer of nurse practitioners, was the recipient of the Crystal Trailblazer Award from the American College of Nurse Practitioners in February 2003. In her acceptance speech, the stated that "in some ways, the profession had expanded beyond my wildest dreams," but cautioned against the medicalization of the NP role: "The profession is rooted in nursing . and we enhance, advance, and influence through nursing. " She added: "Medical and nursing interdependency sets the stage for the highest level of care. No one profession can deliver comprehensive health care themselves. " NNPs, functioning collaboratively with neonatologists as part of a multidisciplinary team, illustrate the truth of Ford's words. They have clearly and unequivocally made significant strides during the past 20 years. Their achievements and contributions have been many but their potential has yet to be reached.
Influence of steam-flaked sorghum grain and supplemental fat on performance of dairy cows in early lactation.

Effects of sorghum grain processing and fat supplementation on milk yield and composition and nutrient digestibilities were studied. Thirty-six Holstein cows were assigned at 5 d of lactation for 91 d to treatment: 1) dry-rolled sorghum, no added fat; 2) dry-rolled sorghum plus Ca salts of long-chain fatty acids; 3) steam-flaked sorghum, no added fat; and 4) steam-flaked sorghum plus Ca salts of long-chain fatty acids. Fat increased ether extract in diets from 5.7 to 7.5% of DM. Milk yield was 4.0 kg/d (12%) higher for cows fed steam-flaked than dry-rolled sorghum. Fat tended to decrease milk yield because of lower DMI, and feed efficiencies increased 15%. Milk protein decreased . 14% on dry-rolled sorghum with added fat, but only . 01% on the steam-flaked diet. Milk protein yield was higher for cows fed flaked than rolled sorghum. Flaking decreased milk fat percentage, but not yield. Added fat decreased shorter chain fatty acids in milk (C4 to C14), but oleic acid increased. Flaking increased C10, C12, and C14, but decreased oleic acid. Digestibilities for DM, CP, starch, and ether extract were higher for flaked and fat-supplemented diets. Flanking of sorghum prevented the milk protein decrease reported for early lactation cows fed added fat.
Comparison of sigma-ligands and metabolic PET tracers for differentiating tumor from inflammation.

UNLABELLED Novel radiopharmaceuticals for the detection of tumors and their metastases may be of clinical interest if they are more tumor selective than (18)F-FDG. Increased glucose metabolism of inflammatory tissues is the main source of false-positive (18)F-FDG PET findings in oncology. METHODS We compared the biodistribution of 4 PET tracers (2 sigma-receptor ligands, (11)C-choline, and (11)C-methionine) with previously published biodistribution data of 3'-deoxy-3'-(18)F-fluorothymidine ((18)F-FLT) and of (18)F-FDG in the same animal model. The model consisted of male Wistar rats that bore tumors (C6 rat glioma in the right shoulder) and also had sterile inflammation in the left calf muscle (induced by injection of 0.1 mL of turpentine). Twenty-four hours after turpentine injection, the rats received an intravenous bolus of PET tracer (approximately 30 MBq in the case of (18)F and 74 MBq for (11)C). RESULTS (18)F-FDG showed the highest tumor-to-muscle ratio of all radiopharmaceuticals (13.2 +/- 3.0, mean +/- SD), followed at a large distance by the sigma-1 ligand (11)C-SA4503 (5.1 +/- 1.7), (18)F-FLT (3.8 +/- 1.3), the non-subtype-selective sigma-ligand (18)F-FE-SA5845 (3.3 +/- 1.5), (11)C-choline (3.1 +/- 0.4), and (11)C-methionine (2.8 +/- 0.3). sigma-Ligands and (18)F-FLT were relatively tumor selective ((18)F-FE-SA5845, greater than 30-fold; (11)C-SA4503 and (18)F-FLT, greater than 10-fold). The tumor selectivity of (11)C-methionine was only slightly better than that of (18)F-FDG. (11)C-Choline showed equal uptake in tumor and inflammation. All tracers were avidly taken up by proliferative tissue (small intestine, bone marrow). High physiologic uptake of some compounds was observed in brain, heart, lung, pancreas, spleen, and salivary gland. CONCLUSION sigma-Ligands and (18)F-FLT were more tumor selective than (18)F-FDG, (11)C-choline, or (11)C-methionine in our animal model. However, these novel radiopharmaceuticals were less sensitive than were the established oncologic tracers.
Nonstationary Noise PSD Matrix Estimation for Multichannel Blind Speech Extraction

Noise power spectral density (PSD) matrix estimation is one of the most important components of a multichannel blind speech extraction framework, as it largely determines the amount of residual noise at the output of a spatial filter. Optimality of well-known spatial filters, such as the multichannel Wiener filter, is only ensured if the PSD matrices of the noise and the desired speech are accurately estimated. In practical situations, where the noise is nonstationary, temporal averaging over time frames where the desired signal is inactive does not provide sufficiently fast tracking of the noise PSD matrix, resulting in high residual noise at the spatial filter output. Therefore, approaches that estimate the PSD matrices using narrowband signal detection have been proposed. Following the well-known single- and multichannel minima-controlled recursive averaging (MCRA) approaches, in this paper, we focus on narrowband speech presence probability-based noise PSD matrix estimators, which are suitable for blind scenarios where the location and the propagation vector of the desired speech source are unknown. The main contributions of the paper are a maximum likelihood interpretation of the multichannel MCRA, and a coherent-to-diffuse ratio-based a priori speech absence probability (SAP) estimator. The latter is a key parameter that determines the accuracy of the noise PSD matrix estimates in nonstationary scenarios. In this paper, we confirm the importance of the a priori SAP and show that its control is crucial for source extraction in nonstationary environments.
Factors of profitability of the grapes production

Th e paper analyses the relationships between the profi tability of viticulture on the one hand and the sugar content and grapes yield in viticulture in the Czech Republic between 1995 and 2014 on the other hand. Th e paper aims to fi nd out which of these has had a greater impact on the viticulture profi tability in the Czech Republic over the last twenty years. One of the frequently debated questions is whether a higher yield of grapes may also bring a high or moderately above average sugar content, and also whether the production for sale is profi table. Th e answer can be achieved either via the micro-appraisal (repeatable and many times conducted experiments) or via the macro-appraisal – collecting average data for the appraised region. For this paper, the macro-appraisal was chosen, i.e. the statistical survey for the Czech Republic between 1995 and 2014. Th e period of 20 years comes from an annual examination conducted by the Association of Winemakers of the Czech Republic on the grapes yields in their members. In the Czech Republic, the price in the grapes market is set depending on the variety of vine and sugar content. It follows from the 20-year statistics of the Association of Winemakers CR, that the profi tability of grapes production for sale is not achieved by the sugar content but by the per hectare yield. Th e yield per hectare should vary between 5.5 and 7 t/ha (tonne per hectare) at the average sugar content of 18.5 to 21 oNM (between 11.0 and 12.5% volume of the potential alcohol). Th e grapes yield of more than 6 t/ha does not increase the profi tability because the price off ered by the purchasing companies decreases.
What Is the Purpose of the Embryonic Heart Beat? or How Facts Can Ultimately Prevail over Physiological Dogma

Embryonic physiology is often viewed as merely those processes understood for the adult but conducted on a smaller physical scale. Yet striking examples of the inaccuracy of this perspective can be identified in the embryonic cardiovascular system. For example, dogma holds that the embryonic heart begins to beat to pump blood for convective transport, just like that of the adult. This is the major assumption inherent in the hypothesis we have called “convective synchronotropy”; that is, the embryonic heart starts to beat synchronously with the need for convective blood flow. However, there is compelling evidence on many fronts that the convective flow of blood generated by the early embryonic vertebrate heart is simply not required for transport of oxygen, nutrients, metabolic wastes, or hormones, all of which can be achieved entirely by diffusion. In fact, fish, amphibian, and bird embryos lacking a functional heart (either through surgical intervention or mutation) or whose oxygen‐hemoglobin transport has been chemically eliminated nonetheless continue to function and grow in size for extended periods up to the point at which diffusion alone can no longer serve oxygen transport needs. We advocate the alternative hypothesis of “prosynchronotropy” (i.e., the heart starts to beat well before convective blood flow is needed for bulk transport). So, what is the purpose of the early embryonic heart beat? Evidence is presented herein in support of a morphogenic rationale for prosynchronotropy. Specifically, it appears that the initial rationale for the beat of the vertebrate embryonic heart may be two‐fold: to aid in subtle but significant aspects of cardiac growth, shaping, and maturation, and to facilitate cardiac maturation angiogenesis—the formation of new vessels by sprouting from vessel tips. Ultimately, the embryonic cardiovascular system provides a graphic demonstration of how adult physiological functions should not, without verification, be interpolated back to the embryo of that species.
Reperfusion after ischemic stroke is associated with reduced brain edema

Rapid revascularization is highly effective for acute stroke, but animal studies suggest that reperfusion edema may attenuate its beneficial effects. We investigated the relationship between reperfusion and edema in patients from the Echoplanar Imaging Thrombolysis Evaluation Trial (EPITHET) and Mechanical Retrieval and Recanalization of Stroke Clots Using Embolectomy (MR RESCUE) cohorts. Reperfusion percentage was measured as the difference in perfusion-weighted imaging lesion volume between baseline and follow-up (day 3–5 for EPITHET; day 6–8 for MR RESCUE). Midline shift (MLS) and swelling volume were quantified on follow-up MRI. We found that reperfusion was associated with less MLS (EPITHET: Spearman ρ = −0.46; P < 0.001, and MR RESCUE: Spearman ρ = −0.49; P < 0.001) and lower swelling volume (EPITHET: Spearman ρ = −0.56; P < 0.001, and MR RESCUE: Spearman ρ = −0.27; P = 0.026). Multivariable analyses performed in EPITHET and MR RESCUE demonstrated that reperfusion independently predicted both less MLS (ß coefficient = −0.056; P = 0.025, and ß coefficient = −0.38; P = 0.028, respectively) and lower swelling volumes (ß coefficient = −4.7; P = 0.007, and ß coefficient = −10.7; P = 0.009, respectively), after adjusting for age, sex, NIHSS, admission glucose and follow-up lesion size. Taken together, our data suggest that even modest improvement in perfusion is associated with less brain edema in EPITHET and MR RESCUE.
A Novel Artificial Anal Sphincter System in an in Vitro and in Vivo Experiment

This paper presents some of the latest progress in the development of a novel artificial anal sphincter system (AASS) to treat severe fecal incontinence. We have redesigned and integrated an intelligent, remote-controlled artificial anal sphincter based on biological signal feedback mechanisms. The device consists of an external telemetry unit, an internal artificial anal sphincter (IAAS), and a transcutaneous energy transfer system (TETS). The mechanical medical micropump of the IAAS can realize bidirectional flow with a maximum flow rate of 8.5 ml/min and can build backpressure up to 170 kPa. The design of the prosthesis reduces occlusion pressure and allows for low inflation volumes (9 mL-10.5 mL); operating pressures between 4.05 kPa and 7.16 kPa indicate that the risk of ischemic injury to the bowel is minimal. Furthermore, the rechargeable battery based on TETS puts the operation time at an estimated 2 days. The performance characteristics of the AASS and its efficiency in achieving continence and sensing the stool inside the anorectum were evaluated in vitro and in vivo in a pig model. Experimental results confirm that the system can maintain continence and build the sense of defecation successfully. Moreover, this innovation can be integrated into not only severe fecal incontinence, erectile dysfunction, and therapy-resistant reflux disease, but also morbid adiposity therapeutic AASS applications.
Supplemental zinc reduced intestinal permeability by enhancing occludin and zonula occludens protein-1 (ZO-1) expression in weaning piglets

The present study was carried out to evaluate the pharmacological effect of Zn in diarrhoea in relation to intestinal permeability. Seventy-two weaning piglets, aged 24 d, were allocated to three dietary treatments: (1) control diet without supplemental Zn; (2) control diet supplemented with 2000 mg Zn/kg from ZnO; (3) control diet supplemented with 2000 mg Zn/kg from tetrabasic zinc chloride (TBZC). At the end of a 14 d experiment period, piglets were weighed, feed consumption was measured, and mucosal barrier function was determined using the lactulose/mannitol test. Expression of mucosal tight junction protein was measured at RNA and protein level. Inclusion of TBZC or ZnO in the diet significantly increased average daily gain (P < 0·01) and average daily feed intake (P < 0·05), while leading to reduced feed conversion ratio (P < 0·05) and faecal scores (P < 0·01). TBZC reduced urinary lactulose:mannitol ratios of weaning piglets (P < 0·05), while dietary supplementation with ZnO tended to reduce urinary lactulose:mannitol ratios (P = 0·061). ZnO or TBZC significantly enhanced the mRNA and protein expression of occludin (P < 0·05) and zonula occludens protein-1 (ZO-1) (P < 0·05) in the ileal mucosa. Piglets fed the TBZC-supplemented diet had a higher level of occludin than pigs fed the ZnO-supplemented diet (P < 0·05). The results indicate that Zn supplementation decreased faecal scores and the reduction was accompanied by reduced intestinal permeability, which was evident from the reduced urinary lactulose:mannitol ratios and increased expression of occludin and ZO-1. Therefore, the protective effect of pharmacological levels of dietary Zn in reducing diarrhoea might, at least partly, be associated with reduced intestinal permeability.
Exogenously Led and Policy-Supported New Path Development in Peripheral Regions: Analytical and Synthetic Routes

Abstract The aim of this article is to explore how new industrial paths emerge and grow in peripheral regional economies. Current conceptualizations of regional path development are based on experiences from core regions and fail to provide satisfactory theoretical explanations of new path-creating activities in peripheral areas. Our conceptual approach combines the notions of path development and knowledge bases, enabling us to distinguish between an analytic and a synthetic route of path creation. We argue that due emphasis should be given to exogenous sources of new path development and policy actions in order to understand how analytical and synthetic routes unfold in peripheral regions. These factors are still underappreciated in prevailing models of path creation. The article contains an analysis of the emergence and evolution of new industries in two peripheral regions in Norway and Austria: the electronics and software industry in Arendal–Grimstad in southeastern Norway, and the software industry in Mühlviertel in Upper Austria. The two industries have developed differently: through the synthetic route based on the emergence and restructuring of manufacturing firms in Arendal–Grimstad and through the analytical route building on the establishment of research facilities in Mühlviertel. Our analysis suggests that the inflow of new analytical and synthetic knowledge from exogenous sources and various types of policy interventions have been vital in sparking the formation of new industrial paths in both regions. The findings clearly challenge uniform, narrowly conceptualized models of industrial evolution and support recent work that advocates a broader theoretical framework to capture exogenously led and policy-supported path-creation processes.
Liposarcomas in Young Patients: A Study of 82 Cases Occurring in Patients Younger Than 22 Years of Age

Liposarcomas typically occur in middle aged to older adults. Altogether, approximately 50 bona fide liposarcomas have been reported in children and adolescents, most of which have represented myxoid liposarcomas, with a good prognosis. We undertook a retrospective study of 82 liposarcomas occurring in patients below 22 years of age. Clinicopathologic and follow-up information was obtained. Fluorescence in situ hybridization for FUS, EWSR1, CHOP (DDIT3), and MDM2 was performed in 30 cases. The tumors occurred in 28 males and 54 females (5 to 22 y of age) and involved many locations. Fifty-six cases were typical myxoid liposarcomas, including 2 with round cell areas. The tumors were grade 1 (56 cases) and grade 3 (2 cases). Thirty-seven of 38 patients with follow-up are alive without disease and 1 is alive with disease (median 59 mo follow-up duration, range: 8 to 108 mo). Six cases showed myxoid liposarcoma with spindled growth (“spindle cell myxoid liposarcoma”); these arose in 5 females and 1 male (median age 14 y) and involved the thigh in 40% of cases. All were grade 1. Follow-up (4 of 6 patients) showed local recurrences in 2 cases and metastases in 1 case. Twelve tumors consisted of conventional myxoid liposarcoma and pleomorphic liposarcoma (“pleomorphic myxoid liposarcoma”); these arose in 4 males and 8 females (10 to 22 y of age) and often involved the mediastinum. Tumor grades were 2 (4 cases) and 3 (8 cases). Follow-up (10 patients) showed 7 dead of disease, 1 alive with disease, and 2 disease free. Four atypical lipomatous tumors were seen including 2 with low-grade dedifferentiation. Two local recurrences were seen; all patients are disease free. Two conventional pleomorphic liposarcomas were seen; 1 patient with follow-up is disease free. FUS-CHOP and EWSR1-CHOP rearrangements were identified by fluorescence in situ hybridization in 15/23 and 2/23 conventional myxoid liposarcomas, respectively, and in no other tumors. Amplification for MDM2 was absent in all cases. We conclude that conventional myxoid liposarcoma is by far the most common subtype of liposarcoma in young patients, with an excellent prognosis. Two apparently novel subtypes of liposarcoma, termed pleomorphic myxoid liposarcoma and spindle cell myxoid liposarcoma comprise considerable percentages of liposarcomas in this age group and should be distinguished from conventional myxoid liposarcoma and conventional pleomorphic liposarcoma. Pleomorphic myxoid liposarcoma and spindle cell myxoid liposarcoma most likely represent high-grade and low-grade variants of myxoid liposarcoma, respectively. Additional study of such cases will be necessary for definitive classification.