Miracle-dz/UniFAR-FTUs huggingface dataset:
Customer Satisfaction and Loyalty as a Part of Customer-Based Corporate Sustainability in the Sector of Mobile Communications Services

In the service sector, the issue of customer satisfaction is being paid great attention. It has become central concern for companies of services to improve service quality, and to maintain customer loyalty within a highly competitive marketplace. The success of customer satisfaction is not only closely related to a company’s quality management and marketing activities but it also has significant impacts on the company’s future and its customer-based corporate sustainability. Customer satisfaction influences variables that are indicators of customer loyalty and long-time customer relationships. The aim of this paper is to provide information about customer satisfaction with quality of electronic communications services and show the results of application of the European customer satisfaction index (ECSI) model carried out in Slovak electronic communications market. Our marketing research pointed out that the ECSI model can provide a better understanding of the complex relationships of the variables of customer loyalty and customer satisfaction and their impact to sustainability of Slovak telecom provider.
Long‐Term Recreational Gymnastics, Estrogen Use, and Selected Risk Factors for Osteoporotic Fractures

The purpose of this cross‐sectional study was to examine whether long‐term participation in recreational gymnastics or folk dancing or estrogen replacement therapy (ERT) is associated with mechanically more competent bones and improved muscular strength and body balance. One hundred and seventeen healthy, female postmenopausal recreational gymnasts (mean age 62.1 [SD 4.7] years) and 116 sedentary controls (mean age 61.5 [4.6] years) were enrolled in the study. Bone mineral content (BMC) of the distal radius, femoral neck, and trochanter were measured with dual‐energy X‐ray absorptiometry. BMC of the midshaft and distal tibia and trabecular density (TrD) of the distal tibia were measured with peripheral computed quantitative tomography. Maximal isometric strength, muscular power, cardiorespiratory fitness, and body balance of the participants were also assessed. The cardiorespiratory fitness, muscular strength, and dynamic balance of the recreational gymnasts and folk dancers combined were significantly better than those of the controls, the average group difference ranging from 7.5% (95% confidence interval 5.0–9.9%) in dynamic balance to 12.8% (6.6–19.4%) in dynamic muscular power. ERT was not associated with the fitness indicators, muscular power, or balance, but was significantly associated with the BMC at all the measured bone sites, the mean group difference between estrogen users and nonusers ranging from 6.5% (3.7–9.3%) for the tibial shaft to 11.8% (6.4–17.0%) for the distal radius. Recreational gymnastics, in turn, was significantly associated with higher BMC at the tibia only, the mean group difference being 3.9% (0.9–6.9%) for the tibial shaft and 7.7% (3.7–11.9%) for the distal tibia. Recreational gymnastics was also associated with higher TrD at the distal tibia (5.2%; 1.2–9.2%), whereas estrogen usage did not show such association. The results indicate that ERT seems especially effective in preventing postmenopausal bone loss, whereas recreational gymnastics and folk dancing improve muscular performance and body balance in addition to increased bone mass and bone size in the tibia. All these factors are essential in prevention of fall‐related fractures of the elderly.
The Impact of Cause Portfolio Focus and Contribution Amount on Stakeholder Evaluations

When companies engage in corporate philanthropy, they can donate to a number of causes supporting a variety of issues, thus establishing cause portfolios. This research examines how the focus of a cause portfolio affects company evaluations. Results from an experiment show that when a company donates a small amount of money, consumers have lower evaluations of a company when the cause portfolio is focused (i.e., supports one issue) versus diverse (i.e., supports many issues). This is because the focused (vs. diverse) portfolio is perceived to have a weaker impact to society. We provide additional evidence of this effect using a data set of Fortune 500 companies’ foundations, showing that cause portfolios are more likely to result in lower stakeholder evaluations when focused (vs. diverse). Again, we find that donation amount alleviates the difference between focused and diverse portfolios. The findings hold important implications for the company’s management of cause portfolios.
Inhibition of apoptosis in rat mesangial cells by tissue inhibitor of metalloproteinase-1.

BACKGROUND Tissue inhibitor of metalloproteinase-1 (TIMP-1) is an important inhibitor of extracellular matrix degradation. Recently, it was reported that TIMP-1 also could inhibit apoptosis in B type lymphocyte. This study was designed to examine the effects of TIMP-1 on mesangial cell apoptosis. METHODS The full-length cDNA of TIMP-1 was cloned and used to construct two recombinant vectors, TIMP-1S and TIMP-1AS, encoding sense TIMP-1 and antisense TIMP-1, respectively. The vectors were transfected into rat mesangial cells (RMC) and their expressions detected by Northern and Western blotting. Apoptosis was induced by serum deprivation, and was monitored for DNA fragmentation by TUNEL assay and DNA laddering. In addition, the expression of endogenous TIMP-1, matrix metalloprotein-2 (MMP-2), and MMP-9, as well as apoptosis-related genes Bcl-2 and Bax were investigated. RESULTS TIMP-1AS transfection induced a suppression of TIMP-1 expression accompanied by an earlier onset of apoptosis, and TIMP-1S transfection induced TIMP-1 over-expression accompanied by a much later onset of apoptosis. A neutralizing antibody of TIMP-1 restored the sensitivity of TIMP-1S-transfected RMC to serum deprivation, but a synthetic matrix metalloproteinase inhibitor BB-94 did not influence the sensitivity of TIMP-1S-transfected RMC to serum deprivation. Finally, TIMP-1 over-expression inhibited the expression of Bax but with no effect on the expression of Bcl-2. CONCLUSION TIMP-1 inhibits the serum deprivation-induced apoptosis in RMC, in which Bax might be involved.
The nucleotide receptor P2X7 mediates actin reorganization and membrane blebbing in RAW 264.7 macrophages via p38 MAP kinase and Rho

Extracellular nucleotides regulate macrophage function via P2X nucleotide receptors that form ligand‐gated ion channels. In particular, P2X7 activation is characterized by pore formation, membrane blebbing, and cytokine release. P2X7 is also linked to mitogen‐activated protein kinases (MAPK) and Rho‐dependent pathways, which are known to affect cytoskeletal structure in other systems. As cytoskeletal function is critical for macrophage behavior, we have tested the importance of these pathways in actin filament reorganization during P2X7 stimulation in RAW 264.7 macrophages. We observed that the P2X7 agonists adenosine 5′‐triphosphate (ATP) and 3′‐O‐(4‐benzoylbenzoyl) ATP (BzATP) stimulated actin reorganization and concomitant membrane blebbing within 5 min. Disruption of actin filaments with cytochalasin D attenuated membrane blebbing but not P2X7‐dependent pore formation or extracellular‐regulated kinase (ERK)1/ERK2 and p38 activation, suggesting that these latter processes do not require intact actin filaments. However, we provide evidence that p38 MAPK and Rho activation but not ERK1/ERK2 activation is important for P2X7‐mediated actin reorganization and membrane blebbing. First, activation of p38 and Rho was detected within 5 min of BzATP treatment, which is coincident with membrane blebbing. Second, the p38 inhibitors SB202190 and SB203580 reduced nucleotide‐induced blebbing and actin reorganization, whereas the MAPK kinase‐1/2 inhibitor U0126, which blocks ERK1/ERK2 activation, had no discernable effect. Third, the Rho‐selective inhibitor C3 exoenzyme and the Rho effector kinase, Rho‐associated coiled‐coil kinase, inhibitor Y‐27632, markedly attenuated BzATP‐stimulated actin reorganization and membrane blebbing. These data support a model wherein p38‐ and Rho‐dependent pathways are critical for P2X7‐dependent actin reorganization and membrane blebbing, thereby facilitating P2X7 involvement in macrophage inflammatory responses.
An Overview of Inflation-Targeting Frameworks: Institutional Arrangements, Decision-making, & the Communication of Monetary Policy

The main objective of this study is to contribute to the public understanding of the inflationtargeting (IT) framework currently being implemented in several leading central banks. We do so by discussing differences in the institutional set up, the decision-making process, and the communication of monetary policy. We analyze these aspects from a conceptual perspective and review them in practice by referring to a set of eleven “small, open” OECD economies as well as four major central banks of the world (Bank of England, Bank of Japan, European Central Bank, & the Federal Reserve). We pay specific attention to recent changes along the multiple dimensions that have, on balance, aimed at further strengthening the IT framework.
The Acute Morphologic Changes That Occur at the Optic Nerve Head Induced by Medical Reduction of Intraocular Pressure

PurposeThe mechanical theory of glaucoma postulates that raised intraocular pressure (IOP) causes laminar distortion resulting in damage to axons at the optic nerve head. There is some evidence that the change in morphology may occur over a short time course. The aim of this paper was to detail the acute morphologic changes at the optic nerve head when IOP was lowered with medical therapy in a clinical population. MethodsSubjects referred to the glaucoma clinic that had a documented IOP of over 28 mm Hg, on no treatment, in one or both eyes were included. Tonometry, pachymetry, and scanning laser ophthalmoscopy were performed before and after lowering IOP with topical apraclonidine and oral acetazolamide. The significance of change in outcome variables was assessed using a paired t test allowing for dependence within subjects and, for sensitivity, with a nonparametric Wilcoxon signed-rank test. ResultsData were obtained from 38 eyes of 19 patients with a mean age of 67 years. After lowering IOP for only 1 hour, there was a significant decrease in mean cup volume (mean change in volume below surface 26.8 μm3, P≤0.001) and mean cup depth (13.8 μm, P<0.01), there being a corresponding increase in mean rim area (37.3 μm2, P<0.034). There was no statistically significant change in central corneal thickness after administration of the IOP lowering medications. ConclusionsIn the present study, it was shown that acute and statistically significant alterations in optic disc morphology occur when IOP is lowered medically with a combination of topical apraclonidine and systemic acetazolamide. The technique, therefore, may be useful to further investigate subgroups of glaucomatous eyes and test etiologic hypotheses.
Upregulation of SENP3/SMT3IP1 promotes epithelial ovarian cancer progression and forecasts poor prognosis

As a crucial member of the small ubiquitin-like modifier system, SUMO-specific protease 3, was identified to be essential for cell proliferation and ribosomal RNA processing. Recent studies showed that SUMO-specific protease 3 was elevated in ovarian cancer compared to normal tissue samples. However, the connection between SUMO-specific protease 3-specific expression and clinicopathological parameters of epithelial ovarian cancer, as well as the physiologically potential role of SUMO-specific protease 3 in epithelial ovarian cancer remained unclear. In this study, an analysis of 124 paraffin-embedded slices by immunohistochemistry indicated that SUMO-specific protease 3 expression was positively correlated with the International Federation of Gynecology and Obstetrics stages (p = 0.025), tumor grade (p = 0.004), and lymph node metastasis (p = 0.001) and was also a critical prognostic factor for the overall survival of epithelial ovarian cancer patients, as revealed by Kaplan–Meier curve analysis. Knockdown of SUMO-specific protease 3 weakened the proliferation, migration, and invasion capability of ovarian cancer cells, down-regulated the expression of Proliferating Cell Nuclear Antigen, Forkhead Box C2, and N-cadherin, and resulted in upregulation of p21 and E-cadherin. Consistent with our results, SUMO-specific protease 3 had been verified to promote cell proliferation, metastasis, and tumorigenesis in multiple malignant cancers, which was a redox-sensitive molecule mediating the epithelial–mesenchymal transition. Collectively, our findings for the first time specifically supported that SUMO-specific protease 3 might play an important role in the regulation of epithelial ovarian cancer progression and could serve as a potential biomarker for prognosis as well as provide a promising therapeutic target against epithelial ovarian cancer.
Radiographic methods used before removal of mandibular third molars among randomly selected general dental clinics.

OBJECTIVES To assess radiographic methods and diagnostically sufficient images used before removal of mandibular third molars among randomly selected general dental clinics. Furthermore, to assess factors predisposing for an additional radiographic examination. METHODS 2 observers visited 18 randomly selected clinics in Denmark and studied patient files, including radiographs of patients who had their mandibular third molar(s) removed. The radiographic unit and type of receptor were registered. A diagnostically sufficient image was defined as the whole tooth and mandibular canal were displayed in the radiograph (yes/no). Overprojection between the tooth and mandibular canal (yes/no) and patient-reported inferior alveolar nerve sensory disturbances (yes/no) were recorded. Regression analyses tested if overprojection between the third molar and the mandibular canal and an insufficient intraoral image predisposed for additional radiographic examination(s). RESULTS 1500 mandibular third molars had been removed; 1090 had intraoral, 468 had panoramic and 67 had CBCT examination. 1000 teeth were removed after an intraoral examination alone, 433 after panoramic examination and 67 after CBCT examination. 90 teeth had an additional examination after intraoral. Overprojection between the tooth and mandibular canal was a significant factor (p < 0.001, odds ratio = 3.56) for an additional examination. 63.7% of the intraoral images were sufficient and 36.3% were insufficient, with no significant difference between images performed with phosphor plates and solid-state sensors (p = 0.6). An insufficient image predisposed for an additional examination (p = 0.008, odds ratio = 1.8) but was only performed in 11% of the cases. CONCLUSIONS Most mandibular third molars were removed based on an intraoral examination although 36.3% were insufficient.
Measurement of femoral and tibial joint reference angles and pelvic limb alignment in cats.

OBJECTIVE To report pelvic limb joint reference angles, limb alignment, and mechanical axis deviation for the feline pelvic limb. STUDY DESIGN Cadaveric radiographic anatomic study. ANIMALS Skeletally mature, mixed breed cats (n = 50). METHODS Extended pelvic limb radiography was performed and images analyzed to determine femoral anatomic joint reference angles, femoral and tibial mechanical joint reference angles, tibiofemoral angle, metatarsotibial angle, mechanical axis-femur and mechanical axis-metatarsus angles, and mechanical axis deviation of the stifle and tarsus. Mean ± SD and 95% confidence intervals (95% CI) were calculated. RESULTS Mean ± SD angles and 95% CI were: anatomic lateral proximal femoral (103.9° ± 3.8°; 103.1° - 104.6°), anatomic lateral distal femoral (93.8° ± 2.5°; 93.3° - 94.3°), mechanical lateral proximal femoral (100.5° ± 3.7°; 99.8° - 101.2°), mechanical lateral distal femoral (97.2° ± 2.2°; 96.7° - 97.6°), mechanical medial proximal tibial (88.0° ± 3.2°; 87.3° - 88.6°), mechanical medial distal tibial (107.6° ± 4.8°; 106.6° - 108.5°), mechanical tibiofemoral (7.4° varus ± 2.5°; 6.9° - 7.9°), mechanical metatarsotibial (6.0° valgus ± 3.5°; 5.3° - 6.7°); mechanical axis-femur (3.2° ± 1.4°; 2.9° - 3.5°) and mechanical axis-metatarsus (2.4° ± 2.0°; 1.7° - 2.1°). Mechanical axis deviation of the stifle and of the tarsus were 2.0% ± 0.9% (95% CI: 1.9-2.2) and 0.8% ± 1.0% (95% CI: 0.7-1.0), respectively. CONCLUSIONS Mean pelvic limb joint reference and limb alignment angles can be used for diagnosing angular limb deformities of the feline pelvic limb and for planning corrective osteotomies in affected cats.
Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues

Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC. However, very little information is available concerning their correspondence in human tissues, and more precisely for human bone marrow. The main objective of this study was therefore to characterize NCSC from adult human bone marrow. In this purpose, we compared human bone marrow stromal cells to human adipose tissue and dermis, already described for containing NCSC. We performed comparative analyses in terms of gene and protein expression as well as functional characterizations. It appeared that human bone marrow, similarly to adipose tissue and dermis, contains NESTIN+ / SOX9+ / TWIST+ / SLUG+ / P75NTR+ / BRN3A+/ MSI1+/ SNAIL1+ cells and were able to differentiate into melanocytes, Schwann cells and neurons. Moreover, when injected into chicken embryos, all those cells were able to migrate and follow endogenous neural crest migration pathways. Altogether, the phenotypic characterization and migration abilities strongly suggest the presence of neural crest-derived cells in human adult bone marrow.
Variable DAXX gene methylation is a common feature of placental trophoblast differentiation, preeclampsia, and response to hypoxia

Placental functioning relies on the appropriate differentiation of progenitor villous cytotrophoblasts (CTBs) into extravillous cytotrophoblasts (EVCTs), including invasive EVCTs, and the multinucleated syncytiotrophoblast (ST) layer. This is accompanied by a general move away from a proliferative, immature phenotype. Genome‐scale expression studies have provided valuable insight into genes that are associated with the shift to both an invasive EVCT and ST phenotype, whereas genome‐scale DNA methylation analysis has shown that differentiation to ST involves widespread methylation shifts, which are counteracted by low oxygen. In the current study, we sought to identify DNA methylation variation that is associated with transition from CTB to ST in vitro and from a noninvasive to invasive EVCT phenotype after culture on Matrigel. Of the several hundred differentially methylated regions that were identified in each comparison, the majority showed a loss of methylation with differentiation. This included a large differentially methylated region (DMR) in the gene body of death domain‐associated protein 6 (DAXX), which lost methylation during both CTB syncytialization to ST and EVCT differentiation to invasive EVCT. Comparison to publicly available methylation array data identified the same DMR as among the most consistently differentially methylated genes in placental samples from preeclampsia pregnancies. Of interest, in vitro culture of CTB or ST in low oxygen increases methylation in the same region, which correlates with delayed differentiation. Analysis of combined epigenomics signatures confirmed DAXX DMR as a likely regulatory element, and direct gene expression analysis identified a positive association between methylation at this site and DAXX expression levels. The widespread dynamic nature of DAXX methylation in association with trophoblast differentiation and placenta‐associated pathologies is consistent with an important role for this gene in proper placental development and function. —Novakovic, B. , Evain‐Brion, D. , Murthi, P., Fournier, T. , Saffery, R. Variable DAXX gene methylation is a common feature of placental trophoblast differentiation, preeclampsia, and response to hypoxia. FASEB J. 31, 2380–2392 (2017). www.fasebj. org
TNK-tissue plasminogen activator compared with front-loaded alteplase in acute myocardial infarction: results of the TIMI 10B trial. Thrombolysis in Myocardial Infarction (TIMI) 10B Investigators.

BACKGROUND Bolus thrombolytic therapy is a simplified means of administering thrombolysis that facilitates rapid time to treatment. TNK-tissue plasminogen activator (TNK-tPA) is a highly fibrin-specific single-bolus thrombolytic agent. METHODS AND RESULTS In TIMI 10B, 886 patients with acute ST-elevation myocardial infarction presenting within 12 hours were randomized to receive either a single bolus of 30 or 50 mg TNK-tPA or front-loaded tPA and underwent immediate coronary angiography. The 50-mg dose was discontinued early because of increased intracranial hemorrhage and was replaced by a 40-mg dose, and heparin doses were decreased. TNK-tPA 40 mg and tPA produced similar rates of TIMI grade 3 flow at 90 minutes (62.8% versus 62.7%, respectively, P=NS); the rate for the 30-mg dose was significantly lower (54.3%, P=0.035) and was 65. 8% for the 50-mg dose (P=NS). A prespecified analysis of weight-based TNK-tPA dosing using median TIMI frame count demonstrated a dose response (P=0.001). Similar dose responses were observed for serious bleeding and intracranial hemorrhage, but significantly lower rates were observed for both TNK-tPA and tPA after the heparin doses were lowered and titration of the heparin was started at 6 hours. CONCLUSIONS TNK-tPA, given as a single 40-mg bolus, achieved rates of TIMI grade 3 flow similar to those of the 90-minute bolus and infusion of tPA. Weight-adjusting TNK-tPA appears to be important in achieving optimal reperfusion; reduced heparin dosing appears to improve safety for both agents. Together with the safety results from the parallel Assessment of the Safety of a New Thrombolytic: TNK-tPA (ASSENT I) trial, an appropriate dose of this single-bolus thrombolytic agent has been identified for phase III testing.
The Impact of Public Expenditures on Economic Growth: A Case Study of Central and Eastern European Countries

ABSTRACT This study tests the importance of various categories of public expenditure, the functional structure, and growth in the gross domestic product (GDP), using an autoregressive-distributed lag (ARDL) model. We document and study the correlation between real GDP growth and 10 different categories of public expenditure, according to their functional classification, using quarterly data for the period 1995–2015, for 10 selected Central and Eastern European countries that joined the European Union. The results of our study, like most recent literature, show that expenditures on education and health care have a positive impact on the economy, while expenditures on defense, economic affairs, general public services, and social welfare have a negative impact.
When a patient commits suicide: An empirical study of psychoanalytic clinicians

Twelve psychoanalysts/psychoanalytic psychotherapists who had a patient commit suicide while in treatment, or shortly after leaving treatment, were interviewed to gain a deeper understanding of the effects of patient suicide on the clinician. A phenomenological research interview was used to gather data, and a psychoanalytic lens was used to interpret the data, generating a descriptive account of the experience of having a patient commit suicide. Thematic analysis of the transcripts yielded eight common themes associated with the clinician's subjective experience of having a patient suicide. The eight themes may be broadly grouped into three general structures: I. Traumatic loss and grief; II. Interpersonal relationships; and III. Professional identity concerns.