Miracle-dz/UniFAR-FTUs huggingface dataset:
Factors Affecting Postural Reduction in Posterior Surgery for Thoracolumbar Burst Fracture

Study Design: Retrospective analysis of a prospectively collected patient database. Objective: To investigate the significance and relevant factors of postural kyphosis reduction during posterior surgical treatment of thoracolumbar burst fracture. Summary of Background Data: Optimal reduction of kyphosis is a goal in the surgical treatment of thoracolumbar burst fracture. Several factors are known to limit the amount of posterior surgical reduction. However, few comprehensive assessments of intraoperative postural reduction have been reported. Methods: Seventy-two consecutive patients who underwent posterior surgical treatment for thoracolumbar (T11–L2) burst fracture were included. Postural reduction was evaluated using C-arm fluoroscopic images and regarded as insufficient when the lateral Cobb angle was ≥20 degrees or AP Cobb angle ≥10 degrees. Clinical characteristics including sex, age, body mass index, time to operation, injury level, and neurological injury, as well as radiologic characteristics including fracture morphology, fracture deformity, canal stenosis, and ligament injuries were investigated to determine the relevant factors. Results: The mean lateral Cobb angle was 22.2±11.0 degrees preoperatively, 16.4±7.7 degrees after postural reduction (P<0.001), and 13.4±6.9 degrees after instrumental reduction (P<0.001). Insufficient postural reduction was found in 25 (34.7%) patients, all of which were lateral. The relevant factors for insufficient reduction, as identified by multivariate analysis, were time to operation >72 hours (OR, 6.453; 95% CI, 1.283–32.553), burst-split type injury (OR, 4.689; 95% CI, 1.314–25.225), and anterior compression ratio >0.5 (OR, 2.284; 95% CI, 1.151–19.811). Conclusions: Postural reduction plays an important role in the reduction of kyphosis and compression deformity after thoracolumbar burst fracture. However, it was affected by delayed operation time, burst-split type injury, and severe anterior vertebral compression.
A 2.4-kilobase-pair fragment of the Friend murine leukemia virus genome contains the sequences responsible for friend murine leukemia virus-induced erythroleukemia

Friend murine leukemia virus (F-MuLV) is a replication-competent, ecotropic, NB-tropic retrovirus which produces a rapidly fatal erythroleukemia in susceptible strains of mice. We previously molecularly cloned the entire F-MuLV genome. Transfection of this cloned DNA into NIH 3T3 mouse fibroblasts produces a virus with the same leukemia-inducing characteristics as F-MuLV. To identify which portion of the F-MuLV genome is responsible for causing leukemia, we made recombinant viruses between subgenomic fragments of F-MuLV DNA and another retrovirus--Amphotroph clone 4070. Amphotroph clone 4070 is a replication-competent, amphotrophic, N-tropic virus which does not produce any detectable malignancy in mice. A 2.4-kilobase-pair fragment of F-MuLV DNA was isolated. This DNA fragment encompassed approximately 700 base pairs from the 3' end of the F-MuLV pol gene and 1.7 kilobase pairs of the env gene including all of gp70 and the N-terminal four-fifths of p15E. A molecularly cloned fragment of Amphotroph DNA was ligated to the 2.4-kilobase-pair F-MuLV DNA, and an 8.3-kilobase-pair hybrid F-MuLV-Amphotroph DNA was subcloned into a new plasmid (p5a25-H). Transfection of p5a25-H DNA into fibroblasts resulted in the production of a replication-competent, ecotropic, N-tropic retrovirus--5a25-H virus. Inoculation of this virus into newborn NIH Swiss mice caused leukemia within 4 to 6 months. The disease caused by 5a25-H was pathologically and histologically indistinguishable from the disease caused by F-MuLV. We conclude that the F-MuLV sequences needed to cause disease are contained in these 2.4 kilobase pairs of DNA.
Stability of Blood Biochemistry Levels in Animal Model Research: Effects of Storage Condition and Time

The purpose of this study was to compare whole blood and plasma in terms of the subsequent accuracy of blood lactate, glucose, lactate dehydrogenase (LDH), creatine phosphokinase (CPK), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) measurement. Blood samples were drawn from 8 male Wistar-Kyoto rats. The rats were homologous, weighed 300— 380 g, were housed in the same environment, and were provided with food and water under the same conditions. Blood draws occurred in all rats at same time. The blood specimens were divided into two samples, one to be stored as whole blood (WBS) and one to be stored as plasma (PS). All the blood sample analyses were performed by trained and experienced personnel to ensure that differences in results were due to variation in form in which specimens were stored rather than to technique. The lactate concentration in the WBS group gradually increased over time, intraclass correlation coefficient (ICC) = 0.541, 95% confidence interval (CI; —0.197, 0.893), and was higher than that of the PS group, ICC = 0.897, 95% CI (0.733, 0.976). By contrast, glucose level gradually declined for the WBS group, ICC= —0.367, 95% CI (—2.563, 0.682). Whole blood storage increased measurement variation for lactate, glucose, LDH, and CPK. Plasma storage prolonged the stability of the biochemical components. This study demonstrates the importance of evaluating validity at each stage of developing and testing animal models.
Multiparametric functional nuclear magnetic resonance imaging shows alterations associated with plasmid electrotransfer in mouse skeletal muscle

In vivo gene electrotransfer is frequently used in preclinical gene therapy. Many studies have attempted to optimize protocols efficiency at the same time as reducing muscle damage. Most of them have reported histological evidence of muscle degeneration and completion of regeneration within 15 days. The functional consequences have rarely been addressed, which may reflect the lack of appropriate techniques. Yet, it is important to characterize the changes induced by the procedure itself because it may interfere with therapy. We used multiparametric functional (mpf)‐nuclear magnetic resonance (NMR) imaging to evaluate mice hindlimb muscle after electrotransfer of an empty plasmid.
Anti-microbial resistance stratified by risk factor among Escherichia coli strains isolated from the urinary tract at a rural clinic in Central India.

BACKGROUND The failure of empirical therapy is frequently observed, even in community-acquired urinary tract infections. We, therefore, conducted a prospective, clinic-based study in 2004-2005 to document anti-microbial resistance rates and correlate them with possible risk factors to assist empirical decision-making. MATERIALS AND METHODS Symptomatic patients with pyuria underwent urine culture. Isolates were identified using standard methods and anti-microbial resistance was determined by disk-diffusion. Ultrasonography was used to detect complicating factors. Patients were stratified by the presence of complicating factors and history of invasive procedures for comparison of resistance rates. STATISTICAL METHOD USED: Chi-square or Fisher exact tests, as appropriate. RESULTS There were 156 E. coli isolates, of which 105 were community-acquired. Twenty-three community-acquired isolates were from patients with complicating factors while 82 were from patients without any. Fifty-one isolates were from patients who had recently undergone invasive procedures on the urinary tract. Thirty-two community-acquired isolates from reproductive-age women without apparent complicating factors had resistance rates of 50% or above against tetracyclines, Co-trimoxazole, aminopenicillins, Nalidixic acid, Ciprofloxacin and 1 st generation cephalosporins. Resistance rates were significantly higher among isolates from patients subjected to invasive procedures, except against Co-trimoxazole, tetracyclines and Amikacin. CONCLUSION High rates of anti-microbial resistance in community-acquired uropathogens have made antimicrobial sensitivity testing necessary even in a rural, primary-care setting.
Integral Projection Models for Species with Complex Demography

Matrix projection models occupy a central role in population and conservation biology. Matrix models divide a population into discrete classes, even if the structuring trait exhibits continuous variation (e.g., body size). The integral projection model (IPM) avoids discrete classes and potential artifacts from arbitrary class divisions, facilitates parsimonious modeling based on smooth relationships between individual state and demographic performance, and can be implemented with standard matrix software. Here, we extend the IPM to species with complex demographic attributes, including dormant and active life stages, cross‐classification by several attributes (e.g., size, age, and condition), and changes between discrete and continuous structure over the life cycle. We present a general model encompassing these cases, numerical methods, and theoretical results, including stable population growth and sensitivity/elasticity analysis for density‐independent models, local stability analysis in density‐dependent models, and optimal/evolutionarily stable strategy life‐history analysis. Our presentation centers on an IPM for the thistle Onopordum illyricum based on a 6‐year field study. Flowering and death probabilities are size and age dependent, and individuals also vary in a latent attribute affecting survival, but a predictively accurate IPM is completely parameterized by fitting a few regression equations. A zip archive of R scripts illustrating our suggested methods is also provided.
Human activity recognition using multisensor data fusion based on Reservoir Computing

Activity recognition plays a key role in providing activity assistance and care for users in smart homes. In this work, we present an activity recognition system that classifies in the near real-time a set of common daily activities exploiting both the data sampled by sensors embedded in a smartphone carried out by the user and the reciprocal Received Signal Strength (RSS) values coming from worn wireless sensor devices and from sensors deployed in the environment. In order to achieve an effective and responsive classification, a decision tree based on multisensor data-stream is applied fusing data coming from embedded sensors on the smartphone and environmental sensors before processing the RSS stream. To this end, we model the RSS stream, obtained from a Wireless Sensor Network (WSN), using Recurrent Neural Networks (RNNs) implemented as efficient Echo State Networks (ESNs), within the Reservoir Computing (RC) paradigm. We targeted the system for the EvAAL scenario, an international competition that aims at establishing benchmarks and evaluation metrics for comparing Ambient Assisted Living (AAL) solutions. In this paper, the performance of the proposed activity recognition system is assessed on a purposely collected real-world dataset, taking also into account a competitive neural network approach for performance comparison. Our results show that, with an appropriate configuration of the information fusion chain, the proposed system reaches a very good accuracy with a low deployment cost.
X-RAY PROPERTIES OF THE FIRST SUNYAEV–ZEL'DOVICH EFFECT SELECTED GALAXY CLUSTER SAMPLE FROM THE SOUTH POLE TELESCOPE

We present results of X-ray observations of a sample of 15 clusters selected via their imprint on the cosmic microwave background from the thermal Sunyaev–Zel'dovich (SZ) effect. These clusters are a subset of the first SZ-selected cluster catalog, obtained from observations of 178 deg2 of sky surveyed by the South Pole Telescope (SPT). Using X-ray observations with Chandra and XMM-Newton, we estimate the temperature, TX, and mass, Mg, of the intracluster medium within r500 for each cluster. From these, we calculate YX = MgTX and estimate the total cluster mass using an M500–YX scaling relation measured from previous X-ray studies. The integrated Comptonization, YSZ, is derived from the SZ measurements, using additional information from the X-ray-measured gas density profiles and a universal temperature profile. We calculate scaling relations between the X-ray and SZ observables and find results generally consistent with other measurements and the expectations from simple self-similar behavior. Specifically, we fit a YSZ–YX relation and find a normalization of 0.82 ± 0.07, marginally consistent with the predicted ratio of YSZ/YX = 0.91 ± 0.01 that would be expected from the density and temperature models used in this work. Using the YX-derived mass estimates, we fit a YSZ–M500 relation and find a slope consistent with the self-similar expectation of YSZ∝M5/3 with a normalization consistent with predictions from other X-ray studies. We find that the SZ mass estimates, derived from cosmological simulations of the SPT survey, are lower by a factor of 0.78 ± 0.06 relative to the X-ray mass estimates. This offset is at a level of 1. 3σ when considering the ∼15% systematic uncertainty for the simulation-based SZ masses. Overall, the X-ray measurements confirm that the scaling relations of the SZ-selected clusters are consistent with the properties of other X-ray-selected samples of massive clusters, even allowing for the broad redshift range (0.29 < z < 1.08) of the sample.
Ammonia excretion of the guinea pig and rabbit.

During fasting in guinea pigs a marked drop of ammonia excretion parallel to a drop in titratable alkalinity was observed. Feeding of cabbage increased ammonia excretion in excess of the titratable alkalinity. In acute studies in guinea pigs and rabbits after administration of saline, bicarbonate or hydrochloric acid, a depression of ammonia excretion was consistently observed whenever the urinary pH was close to neutrality, while in acid as well as in alkaline urines an increase in ammonia excretion occurred. When the urine flow was very low (
Detection of HCV RNA in the Peripheral Blood Mononuclear Cells of Serum HCV RNA-Negative Egyptian Patients Under Interferon Treatment

Introduction:Despite recent success after the introduction of combination therapy with interferon (IFN)-&agr; and ribavirin, approximately 60% of patients with hepatitis C virus (HCV) genotype 4 fail to respond. Resistance to antiviral therapy remains a serious problem in the management of chronic hepatitis C. In most patients, HCV RNA could be detected in peripheral blood mononuclear cell (PBMC). Objective:The authors aimed to investigate the predictive value of HCV RNA in PBMC of patients with chronic hepatitis C after IFN treatment, which may act as the source of HCV reinfection of hepatic cells. Methods:Seventy patients with chronic hepatitis C were treated with IFN plus ribavirin for 48 weeks; they all achieved clearance of HCV RNA from serum. At the end of treatment, PBMC and serum were examined by real-time polymerase chain reaction for detection of HCV RNA. Six months later, HCV RNA in serum was monitored to detect sustained virologic response. Results:Analysis revealed the presence of detectable HCV RNA in the PBMC of 27% of patients despite clearance of serum HCV RNA. During follow-up, 80% of the patients who became serum HCV positive 6 months after the end of treatment had detectable level of HCV RNA in PBMC at the end of treatment. Conclusions:The absence of HCV in the serum of patients by the end of treatment does not exclude future viremia. The patient might still be a source of infection to others. It is strongly encouraged to test for HCV in PBMC to detect lack of response to treatment and persisting infection.
Lectins with Anti-HIV Activity: A Review

Lectins including flowering plant lectins, algal lectins, cyanobacterial lectins, actinomycete lectin, worm lectins, and the nonpeptidic lectin mimics pradimicins and benanomicins, exhibit anti-HIV activity. The anti-HIV plant lectins include Artocarpus heterophyllus (jacalin) lectin, concanavalin A, Galanthus nivalis (snowdrop) agglutinin-related lectins, Musa acuminata (banana) lectin, Myrianthus holstii lectin, Narcissus pseudonarcissus lectin, and Urtica diocia agglutinin. The anti-HIV algal lectins comprise Boodlea coacta lectin, Griffithsin, Oscillatoria agardhii agglutinin. The anti-HIV cyanobacterial lectins are cyanovirin-N, scytovirin, Microcystis viridis lectin, and microvirin. Actinohivin is an anti-HIV actinomycete lectin. The anti-HIV worm lectins include Chaetopterus variopedatus polychaete marine worm lectin, Serpula vermicularis sea worm lectin, and C-type lectin Mermaid from nematode (Laxus oneistus). The anti-HIV nonpeptidic lectin mimics comprise pradimicins and benanomicins. Their anti-HIV mechanisms are discussed.
Fabrication and characterization of microstructures created in thermally deposited arsenic trisulfide by multiphoton lithography

Abstract. A detailed study of multiphoton lithography (MPL) in arsenic trisulfide (As2S3) films and the effects on nanoscale morphology, chemical networking, and the appearance of the resulting features by the chemical composition, deposition rate, etch processing, and inclusion of an antireflection (AR) layer of As2Se3 between the substrate and the As2S3 layer is reported. MPL was used to photo-pattern nanostructured arrays in single- and multilayer films. The variation in chemical composition for laser-exposed, UV-exposed, and unexposed films is correlated with the etch response, nanostructure formation, and deposition conditions. Reflection of the focused beam at the substrate back into the film produces standing wave interference that modulates the exposure with distance from the substrate and produces nanobead structures. The interference and the modulation can be controlled by the addition of an AR layer of As2Se3 deposited between the substrate and the As2S3 film. Relative to structures produced in a single-layer As2S3 film having no AR layer, photo-patterning in the multilayer As2S3-on-As2Se3 film yields pillar-shaped structures that are closer to the targeted shape and are narrower (120 versus 320 nm), more uniform, and better adhering to the substrate. Processing methods are demonstrated for fabricating large-area arrays with diffractive optical function.
Erythema induratum: a clinicopathologic and polymerase chain reaction study.

BACKGROUND In Taiwan, cutaneous lesions with granulomatous lobular panniculitis, with or without vasculitis, are usually diagnosed as erythema induratum (EI), a common form of tuberculid associated with tuberculosis. However, there has been no study to elucidate the extent of this association in Taiwan. The aim of this study was to document the spectrum of the pathologic findings in EI and its association with Mycobacterium tuberculosis. METHODS The diagnostic/inclusion criteria for EI were recurrent tender subcutaneous nodules on the legs, histopathologic findings of granulomatous lobular or septolobular panniculitis plus necrosis or vasculitis, and positive response to antituberculosis therapy. The clinicopathologic findings in the 12 cases that fulfilled these criteria were analyzed, and nested polymerase chain reaction (PCR) was used to identify M. tuberculosis complex DNA from formalin-fixed, paraffin-embedded sections. RESULTS Eleven women and one man were included in the study, ranging from 18 to 70 years old (mean, 40.6 yr). The duration of the disease ranged from 10 days to 10 years (mean, 2.1 yr). Histopathology revealed granulomatous panniculitis; a diffuse lobular pattern was observed in nine cases and a focal lobular/septolobular pattern in three. Vasculitis was found in nine cases, four affecting an artery or vein, with three occurring in the patients with focal panniculitis. Ten cases showed various degrees of caseous necrosis. Eosinophils or focal eosinophilia were fairly common (10 patients). From PCR, nine patients were positive for M. tuberculosis complex DNA. CONCLUSIONS Type III and IV hypersensitivity reactions to M. tuberculosis complex were involved in the pathogenesis of EI. Our results suggest that approximately half of the cases with pathologic findings consistent with EI or nodular vasculitis in Taiwan are associated with M. tuberculosis.
Discriminant analysis of the Ullrich-Turner syndrome neurocognitive profile.

Ullrich-Turner syndrome (UTS), or monosomy X, is a genetic disorder characterized by short stature, gonadal dysgenesis, and a particular neurocognitive profile of normally developed language abilities (particularly verbal IQ) and impaired visual-spatial and/or visual-perceptual abilities. The most frequently described profile in UTS includes difficulty with tasks involving memory and attention, decreased arithmetic skills, and impaired visual spatial processing. We used discriminant function analysis (DFA) to distinguish between the neurocognitive profiles of girls with UTS vs. controls matched for age, height, IQ, and socioeconomic status. DFA is a statistical method for deriving a linear function that optimally weights parameters to permit sensitive and specific differentiation among groups. We developed a modified discriminant function, based on seven cognitive test scores, that successfully discriminated between the UTS and control subjects with a sensitivity of 0.45 and a specificity of 0.97. To validate its performance, we applied the discriminant function to a small group of 45,X UTS subjects (n = 13) and control female subjects (n = 25), ages 7-16 years, who were not part of the previous analyses. The discriminant function (DF) identified 54% of these 13 UTS subjects as having the "UTS neurocognitive profile" and 92% of the 25 control subjects as not having the profile. We also compared the DF scores of UTS girls with various mosaic karyotypes and found that the group with 46,XX mosaicism had significantly higher scores (i.e., closer to normal controls) than the other two mosaic groups (t = 2.86, P < 0.005). The results of this study should be useful for genetic counseling and planning educational programs for girls with UTS.
Stress‐induced barrier disruption of rat follicle‐associated epithelium involves corticotropin‐releasing hormone, acetylcholine, substance P, and mast cells

Background The follicle‐associated epithelium (FAE) is specialized in uptake and sampling of luminal antigens and bacteria. We previously showed that stress increased FAE permeability in rats. An increased uptake may alter antigen exposure in Peyer’s patches leading to intestinal disease. The aim of this study was to elucidate mechanisms involved in the acute stress‐induced increase in FAE permeability.
EFFECT OF SOME PHYSIOLOGICAL FACTORS ON BLOOD PARAMETERS IN CATFISH ( CLARIAS LAZERA ) 1

The effects of environmental changes on blood parameters of adult male and female catfish (Clarias lazera ) were tested. The effects of fasting, dim light, high temperatures, and airless on the haemogram, leucogram, coagulation parameters and stress indicating tests (glucose and cortisol levels). The results revealed that, in control fish the average red blood cells(RBCs) count (was 1.55 X 10 6 / mm 3 , hemoglobin(Hb) concentration was 8.2 g/dl and packed cell volume (PCV) was 35.77%. The average white blood cell(WBCs) count was 1.89 X 10 4 / mm 3 and the percentages of heterophils, basophils, eosinophils, monocytes and lymphocytes were 3.9%, 0.33%, 4.47%, 5.2% and 82%, respectively. The clotting time was 100 seconds, prothrombin time was 38.69 second, thrombocyte count was 3.33 10 4 /mm 3 and serum calcium level was 2.28 mmol/l. The average plasma glucose level was 3.77 mmol/1 and cortisol level was 26.6 nmol/l. Environmentally induced changes in leukocytes and coagulation parameters were negligible. However, fasting and dim light induced a significant decrease in RBCs, Hb and PVC, whereas these parameters were increased markedly after exposure to high temperature and airless. Hyperglycemia was recorded after high temperature, while under fasting condition hypoglycemia was resulted. Cortisol level significantly increased under all conditions of the present study except in dim light it was significantly decreased. In conclusion of the environmental factors tested, airless condition and fasting caused the greatest changes in the blood parameters relative to dim light and high temperature.