Miracle-dz/UniFAR-FTUs huggingface dataset:
C-er b-B 2 ( HER 2 / neu ) EXPRESSION IN SYNOVIAL SARCOMA OF THE HEAD AND NECK

Background. Synovial sarcoma is a malignant mesenchymal tumor composed of varying proportions of spindle and epithelial cell components. Because of the histologic and immunohistochemical similarity of synovial sarcoma to epithelial carcinomas, we hypothesized that the human epithelial growth factor receptor 2 (C-erb-B2, also termed HER2/neu) may contribute to the tumor phenotype and provide a new therapeutic target for this soft tissue tumor. Methods. Three head and neck, one chest wall, and seven extremity synovial sarcomas were evaluated for C-erb-B2 (HER2/ neu) expression by immunohistochemistry, Western immunoblotting, and fluorescence in situ hybridization (FISH). Results. The head and neck cases demonstrated immunohistochemically strong positive staining, whereas tumors from other anatomic locations showed neither positive nor cytoplasmic restricted staining. Antigen-targeted antibody therapy (trastuzumab) was initiated in two patients. Conclusions. These results demonstrate that C-erb-B2 (HER2/neu) may play a role in the tumorigenesis of synovial sarcoma; and, therefore, antigrowth factor therapies may provide a previously unrecognized pharmaceutical approach to soft tissue tumors. The data also suggest that although synovial sarcoma of the head and neck and synovial sarcoma of the extremities have similar morphologic features, they may be clinically and mechanistically distinct entities. A 2005 Wiley Periodicals, Inc. Head Neck 27: 883–892, 2005
Biodiversity of 20 chicken breeds assessed by SNPs located in gene regions

Twenty-five single nucleotide polymorphisms (SNPs) were analyzed in 20 distinct chicken breeds. The SNPs, each located in a different gene and mostly on different chromosomes, were chosen to examine the use of SNPs in or close to genes (g-SNPs), for biodiversity studies. Phylogenetic trees were constructed from these data. When bootstrap values were used as a criterion for the tree repeatability, doubling the number of SNPs from 12 to 25 improved tree repeatability more than doubling the number of individuals per population, from five to ten. Clustering results of these 20 populations, based on the software STRUCTURE, are in agreement with those previously obtained from the analysis of microsatellites. When the number of clusters was similar to the number of populations, affiliation of birds to their original populations was correct (>95%) only when at least the 22 most polymorphic SNP loci (out of 25) were included. When ten populations were clustered into five groups based on STRUCTURE, we used membership coefficient (Q) of the major cluster at each population as an indicator for clustering success level. This value was used to compare between three marker types; microsatellites, SNPs in or close to genes (g-SNPs) and SNPs in random fragments (r-SNPs). In this comparison, the same individuals were used (five to ten birds per population) and the same number of loci (14) used for each of the marker types. The average membership coefficients (Q) of the major cluster for microsatellites, g-SNPs and r-SNPs were 0.85, 0.7, and 0.64, respectively. Analysis based on microsatellites resulted in significantly higher clustering success due to their multi-allelic nature. Nevertheless, SNPs have obvious advantages, and are an efficient and cost-effective genetic tool, providing broader genome coverage and reliable estimates of genetic relatedness.
Nitric-oxide Dioxygenase Activity and Function of Flavohemoglobins

Widely distributed flavohemoglobins (flavoHbs) function as NO dioxygenases and confer upon cells a resistance to NO toxicity. FlavoHbs from Saccharomyces cerevisiae,Alcaligenes eutrophus, and Escherichia colishare similar spectra, O2, NO, and CO binding kinetics, and steady-state NO dioxygenation kinetics. Turnover numbers (V max) for S. cerevisiae, A. eutrophus, and E. coli flavoHbs are 112, 290, and 365 NO heme−1 s−1, respectively, at 37 °C with 200 μm O2. The K M values for NO are low and range from 0.1 to 0.25 μm. V max/K M (NO) ratios of 900–2900 μm −1 s−1 indicate an extremely efficient dioxygenation mechanism. ApproximateK M values for O2 range from 60 to 90 μm. NO inhibits the dioxygenases at NO:O2ratios of ≥1:100 and makes true K M (O2) values difficult to determine. High and roughly equal second order rate constants for O2 and NO association with the reduced flavoHbs (17–50 μm −1 s−1) and small NO dissociation rate constants suggest that NO inhibits the dioxygenase reaction by forming inactive flavoHbNO complexes. Carbon monoxide also binds reduced flavoHbs with high affinity and competitively inhibits NO dioxygenases with respect to O2(K I (CO) = ∼1 μm). These results suggest that flavoHbs and related hemoglobins evolved as NO detoxifying components of nitrogen metabolism capable of discriminating O2 from inhibitory NO and CO.
Amyloid‐Aβ Peptide in Olfactory Mucosa and Mesenchymal Stromal Cells of Mild Cognitive Impairment and Alzheimer's Disease Patients

Patients with mild cognitive impairment (MCI) or Alzheimer's disease (AD) might develop olfactory dysfunction that correlates with progression of disease. Alteration of olfactory neuroepithelium associated with MCI may be useful as predictor of cognitive decline. Biomarkers with higher sensitivity and specificity would allow to understand the biological progression of the pathology in association with the clinical course of the disease. In this study, magnetic resonance images, apolipoprotein E (ApoE) load, Olfactory Connecticut test and Montreal Cognitive Assessment (MoCA) indices were obtained from noncognitive impaired (NCI), MCI and AD patients. We established a culture of patient‐derived olfactory stromal cells from biopsies of olfactory mucosa (OM) to test whether biological properties of mesenchymal stromal cells (MSC) are concurrent with MCI and AD psychophysical pathology. We determined the expression of amyloid Aβ peptides in the neuroepithelium of tissue sections from MCI and AD, as well as in cultured cells of OM. Reduced migration and proliferation of stromal (CD90+) cells in MCI and AD with respect to NCI patients was determined. A higher proportion of anosmic MCI and AD cases were concurrent with the ApoE ε4 allele. In summary, dysmetabolism of amyloid was concurrent with migration and proliferation impairment of patient‐derived stem cells.
Influence of recovery time on post-activation potentiation in professional rugby players

Abstract Following a bout of heavy resistance training, the muscle is in both a fatigued and potentiated state with subsequent muscle performance depending on the balance between these two factors. To date, there is no uniform agreement about the optimal acute recovery required between the heavy resistance training and subsequent muscle performance to gain performance benefits. The aim of the present study was to determine the recovery time required to observe enhanced muscle performance following a bout of heavy resistance training. Twenty professional rugby players performed a countermovement jump at baseline and ∼15 s, 4, 8, 12, 16, 20, and 24 min after a bout of heavy resistance training (three sets of three repetitions at 87% one-repetition maximum squat). Power output, jump height, and peak rate of force development were determined for all countermovement jumps. Despite an initial decrease in countermovement jump performance after the heavy resistance training (P < 0.001), participants' performance increased significantly following 8 min recovery (P < 0.001) (i.e. jump height increased by 4.9%, s = 3.0). The findings suggest that muscle performance during a countermovement jump can be markedly enhanced following bouts of heavy resistance training provided that adequate recovery (∼8 min) is allowed between the heavy resistance training and the explosive activity.
Differential effects of IL‐12 on the generation of alloreactive CTL mediated by murine and human dendritic cells: a critical role for nitric oxide

We examined the mechanisms involved in interleukin (IL)‐12‐mediated suppression of cellular immunity in mice using allogeneic mixed leukocyte reaction (MLR) stimulated by dendritic cells (DCs) in vitro and compared the effect of IL‐12 on MLR in mice and humans. Although IL‐12 stimulated human MLR, the addition of IL‐12 or interferon‐γ (IFN‐γ) resulted in a dose‐dependent suppression of MLR in mice. The treatment with NG‐monomethyl‐l‐arginine (L‐NMMA) completely abrogated IL‐12‐ and IFN‐γ‐mediated suppression of MLR in mice. Furthermore, IL‐12 enhanced the alloreactive cytolytic T lymphocyte (CTL) induction in human MLR, whereas the addition of L‐NMMA was required to generate alloreactive CTLs in the presence of IL‐12 in mice. Nitric oxide (NO) was detected only in mouse MLR. Murine DCs could produce NO, but neither human CD34+ cell‐ nor monocyte‐derived DCs produced a detectable amount of NO. These results suggest that NO produced by DCs might play an important role in IL‐12‐mediated immune suppression in mice but not in humans.
Redefined Blood Pressure Variability Measure and Its Association With Mortality in Elderly Primary Care Patients

Visit-to-visit blood pressure (BP) variability has received considerable attention recently. The objective of our study is to define a variability measure that is independent of change over time and determine the association between longitudinal summary measures of BP measurements and mortality risk. Data for the study came from a prospective cohort of 2906 adults, aged ≥60 years, in an urban primary care system with ⩽15 years of follow-up. Dates of death for deceased participants were retrieved from the National Death Index. Systolic and diastolic BP measurements from outpatient clinic visits were extracted from the Regenstrief Medical Record System. For each patient, the intercept, regression slope, and root mean square error for visit-to-visit variability were derived using linear regression models and used as independent variables in Cox proportional hazards models for both all-cause mortality and mortality attributable to coronary heart disease or stroke. Rate of change was associated with mortality risk in a U-shaped relationship and that participants with little or no change in BP had the lowest mortality risk. BP variability was not an independent predictor of mortality risk. By separating change over time from visit-to-visit variability in studies with relatively long follow-up, we demonstrated in this elderly primary care patient population that BP changes over time, not variability, were associated with greater mortality risk. Future research is needed to confirm our findings in other populations.
Potentiation of conditioned freezing following dorsomedial prefrontal cortex lesions does not interfere with fear reduction in mice.

In Experiment 1, an auditory conditioned stimulus (CS) was paired with footshock, except when it was preceded by another stimulus (a visual conditioned inhibitor [CI]). After conditioning, all mice displayed less CS-evoked freezing when the CI-CS compound was presented than when the CS was presented alone. However, lesions of the dorsomedial prefrontal cortex (dmPFC) potentiated CS-evoked freezing on each of the 2 sessions (i.e., CI CS and CS alone). In Experiment 2, mice were submitted to fear extinction (CS-alone presentation for 3 days). Lesioned mice exhibited a higher level of freezing behavior than controls on each of the 3 sessions. However, lesioned mice and controls displayed the same rate of reduction of freezing over the 3 days of extinction. These data in mice support previous studies in rats, which suggests that the dmPFC is not critical for either conditioned inhibition or extinction of acquired freezing behavior.
Occupational Medicine Practice: Activities and Skills of a National Sample

Objective: To characterize activities and skills of occupational physicians using work diaries. Methods: A total of 260 occupational physicians from a national sample provided task/skill descriptions at approximately 25 specific times. The average percentage of activity samples using a skill and the interquartile range expressed results. Results: Clinical activities, particularly musculoskeletal, were most frequent, followed by industry and health system management. Traditional public health approaches were infrequent. Injured patients, employers, and healthy workers were the most common beneficiaries. Communication about prevention and work restrictions was frequent. Interphysician variability was high for most measures. Conclusions: Results demonstrated a dichotomy—many frequent activities/skills are associated with other specialties as well (eg, treating injury); others, albeit less frequently used, demarcate the uniqueness of occupational medicine (eg, preventive examinations, toxicology, benefiting employers or worker groups, assessing work ability, payment by employers).
An assessment of non-timber forest products (NTFPs) utilization on rural livelihoods in Ini local government area of Akwa Ibom State, Nigeria

The study examined the contribution of non-timber forest products (NTFPS) utilization on rural livelihood in Ini Local Government Area of Akwa Ibom State, Nigeria. The specific objective was to investigate the utilization of NTFPs and ascertains it effects on rural livelihood. Data were obtained through the administration of 373 copies of structured questionnaire to 10 communities with large portion of natural rainforest. Data obtained were analysed using descriptive and inferential statistical tools. The multiple regression result showed that the utilization of NTFPs was significantly influenced by socio-economic factors (age, gender, income and educational level) (p<0.05). The t-statistic result revealed that the utilization of NTFPs greatly contributed to rural livelihood such as cooking, medical treatment, roofing materials, craft making and income generation (p<0.01). The study revealed that 96.7 per cent of forest lands in the area were owned by individuals, families and communities, while only 3.3 per cent was owned either by the state or local government. The study suggests the domestication of economically valuable products be given priority at the local level as well as improves trade in NTFPs.
Cytomegalovirus Retinitis in an ALL Child on Exclusive Chemotherapy Treated Successfully With Intravitreal Ganciclovir Alone

A child suffering from acute lymphoblastic leukemia on treatment with exclusive chemotherapy presented with vision-threatening cytomegalovirus (CMV) retinitis in 1 eye. Prompt diagnosis and treatment with 3 weekly doses of 2 mg/0.1 mL intravitreal ganciclovir resulted in successful healing of CMV retinitis with restoration of visual acuity. In children with acute lymphoblastic leukemia on exclusive chemotherapy without hematopoietic stem cell transplantation, CMV retinitis has been reported in only 1 case in literature. This child was treated successfully with intravenous ganciclovir. This report highlights the use of successful intravitreal ganciclovir in pediatric age group to avoid side effects of systemic ganciclovir.
Cardiac Injury Markers in Non-elite Marathon Runners

An elevation of cardiac injury markers including creatinine kinase (CK), myoglobin (Myo) and cardiac troponin T (cTnT) has been observed in elite athletes following strenuous exercise. The mechanism and significance of this observation however have not been fully elucidated. The goals of this study were: 1) to determine whether these changes in biomarkers also occur in a large, heterogeneous group of non-elite athletes; and 2) to identify possible clinical or biochemical associations. We recruited 129 non-elite runners in 2006, 61 individuals who were taking part in the half (13.1 miles) marathon and 68 individuals participating in the full (26.2 miles) marathon. Demographic data and blood samples were collected for analysis of CK, Myo, cTnT, and Creatinine (Cr) levels within two hours of race start, at race completion, and 1-h post-race for both patient cohorts. In the 61 individuals (40 males, 40+/-12 yrs) completing the half marathon in a mean time of 150+/-20 min, 90.3%, 65.2%, and 30.6% of the subjects exhibited significant elevations in Myo, CK, and cTnT, respectively immediately post race and 100%, 74.9% and 45.9% in the same biomarkers one hour-post race. In the 68 individuals (44 males, 42+/-14 yrs) completing the full marathon in a mean time of 310+/-30 min, 95.3%, 70.2% and 35.7% exhibited significant elevations in Myo, CK and cTnT respectively immediately post race and 100%, 78.5% and 52.8% in the same biomarkers one hour-post race. The elevation in cTnT levels post-race were modestly associated with the time required to complete the race for the entire cohort of marathon runners. The serum levels of Cr, CK, and Myo post-race did not correlate however with age, sex, BMI, level of training, or prior marathon experience. Elevations of cardiac injury markers in non-elite athletes are extremely common following the completion of endurance events and correlate to the increased endurance time. Whether the increase in the levels of these enzymes represents true myocardial injury or a result of the release of cTnT from the myocytes requires further investigation.
Cadmium in Placenta—A Valuable Biomarker of Exposure During Pregnancy in Biomedical Research

Rats were exposed to cadmium (Cd) during pregnancy orally (50 ppm Cd in drinking water from gestation day [GD] 1–20) or parenterally (5 mg/kg sc during GD 1–19 or on GD 16). Cadmium levels were measured in blood, placenta, and fetus at the end of exposure. Human studies comprised of two cohorts of healthy Croatian postpartum women in the continental versus coastal area; in these women, Cd levels were measured in maternal blood, placenta, and cord blood. Cadmium in rat placenta was 10-fold higher following parenteral versus oral exposure, with highest value after exposure in mid pregnancy. The values were 10-fold higher in placenta than blood, and the fetus had ≤10% of blood metal levels. Cadmium levels in human placenta noted increased values in smokers that were 10- to 20-fold higher than in maternal blood and in cord blood were very low (<0.1 ppb). In nonsmokers, different Cd levels in placenta and blood in coastal versus continental area were found due to dietary intake. Data showed that Cd in placenta is a reliable biomarker of exposure following oral and parenteral treatment during pregnancy in rats and in human biomonitoring that includes prenatal assessment. In addition, tobacco smoking is a prominent source of metal exposure. Cd levels in placenta are also a valuable biomarker of metal dietary exposure related to specific dietary habits and soil characteristics.
T cell activation correlates with an increasedproportion of antigen among the materials acquiredfrom target cells

We have investigated the density of peptides required to elicit different biological responses in cytotoxic T lymphocytes (CTL), including trogocytosis (i.e., the phenomenon whereby the lymphocytes actively capture fragments of plasma membrane from those cells with which they establish an immune synapse). We have used two separate mouse models of CTL recognising defined peptides presented by MHC class I molecules. In both systems, triggering of cytotoxicity and capture of membrane components reached saturation with low densities of ligand. On the other hand, down‐modulation of cell‐surface levels of TCR, induction of IFN‐γ production and detection of peptide captured required much higher ligand densities. Interestingly, fratricide (i.e., killing between CTL sharing the same specificity), a mechanism proposed to account for CTL exhaustion, was detected only at antigen concentrations still well above that second threshold leading to full blown activation. Taken together, our results show that the different thresholds that govern the elicitation of different CTL functions correlate with different proportions of antigen among the target cell components being captured via trogocytosis.
Exchange Rates and Monetary Fundamentals: What Do We Learn from Long-Horizon Regressions?

The use of a new bootstrap method for small-sample inference in long-horizon regressions is illustrated by analysing the long-horizon predictability of four major exchange rates, and the findings are reconciled with those of an earlier study by Mark (1995). While there is some evidence of exchange rate predictability, contrary to earlier studies, no evidence is found of higher predictability at longer horizons. Additional evidence is presented that the linear VEC model framework underlying the empirical study is likely to be misspecified, and that the methodology for constructing bootstrap p-values for long-horizon regression tests may be fundamentally flawed.
The AMPK-FoxO3A axis as a target for cancer treatment

FoxO proteins are an evolutionarily conserved subfamily of transcription factors involved in tumor suppression, regulation of energy metabolism and development in several tissues, and are mainly regulated by phosphorylation-dependent nuclear/cytoplasmic shuttling. The transcriptional activity of FoxO3A, one of the four members of the family, is further modulated by AMPK, one of the key regulators of cellular metabolism, which basically shifts cell machinery from energy-consuming to energy-producing pathways. We recently demonstrated that the AMPK/FoxO3A energy sensor pathway is still inducible in human cancer cells in response to metabolic stress, as it becomes activated in colorectal and ovarian cancer cells in response to the inhibition of p38α. Activation of the FoxO3A transcriptional program initially induces autophagy as an attempt to retain energy to survive, whereas under persistent stress conditions it triggers autophagic cell death. In this review, we focus on the connections between AMPK and FoxO3A, describing their central role as modulators of fundamental processes such as stress resistance, cell metabolism, autophagy and cell death, and highlighting the therapeutic potential of pharmacological modulation of the AMPK-FoxO3A axis.
Double-blind randomized study comparing amoxicillin and erythromycin for the treatment of Chlamydia trachomatis in pregnancy.

OBJECTIVE To compare the efficacy and patient tolerance of amoxicillin to that of erythromycin in the treatment of lower genital tract chlamydia infections during pregnancy. METHODS A double-blind, randomized study was conducted comparing oral amoxicillin 500 mg three times daily versus oral erythromycin 500 mg four times daily for 7 days. One hundred forty-three women with positive cervical cultures for chlamydia at less than 36 weeks' gestation were enrolled. A test-of-cure culture was obtained 4 weeks after entry into the study and side effects were assessed. Success of the regimen was defined as completing the course of medication and having a negative test-of-cure culture. RESULTS Thirty of the 65 women in the erythromycin group (46.1%) developed symptoms while taking the medication and 15 of them were unable to continue treatment (23.1%). In contrast, five of the 65 women (7.7%) in the amoxicillin group became symptomatic, with only one of these patients intolerant of the side effects (1.5%) (P < .001). Of the 50 patients in the erythromycin group who were able to complete their course of medication, only three had a positive test of cure (6.0%). In comparison, nine of the 64 patients (14.1%) taking amoxicillin who completed their course had positive cultures at test of cure. This difference was not statistically significant (P = .14). Forty-seven of the 65 patients (72.3%) in the erythromycin group successfully completed their regimen, compared to 55 of the 65 women (84.6%) in the amoxicillin group. This difference was not statistically significant. CONCLUSIONS These findings suggest that amoxicillin is a reasonable alternative for the treatment of chlamydia in pregnant patients intolerant to erythromycin. The incidence of side effects and intolerance to therapy for amoxicillin are less than those for erythromycin.