Miracle-dz/UniFAR-FTUs huggingface dataset:
Use of the oligochaete, Lumbriculus variegatus, as a prey organism for toxicant exposure of fish through the diet

The oligochaete, Lumbriculus variegatus, has several characteristics that make it desirable as a prey organism for conducting dietary exposure studies with fish. We conducted 21‐ and 30‐d experiments with young fathead minnows (Pimephales promelas) and rainbow trout (Oncorhynchus mykiss), respectively, to determine whether a diet consisting solely of L. variegatus would support normal growth and to compare performance with standard diets (Artemia nauplii, frozen brine shrimp, or trout chow). All diets were readily accepted, and fish survived and grew well. Food conversion in both fathead minnows and rainbow trout was as high as or higher for the oligochaete diet compared with others, although this comparison is influenced by differences in ration, ingestion rate, or both. The oligochaete diet had gross nutritional analysis similar to the other diets, and meets fish nutrition guidelines for protein and essential amino acids. Methodologies and practical considerations for successfully using oligochaetes as an experimental diet are discussed. Considering their ready acceptance by fish, their apparent nutritional sufficiency, the ease of culturing large numbers, and the ease with which they can be loaded with exogenous chemicals, we believe that L. variegatus represents an excellent choice of exposure vector for exposing fish to toxicants via the diet.
Identification of human placenta‐derived mesenchymal stem cells involved in re‐endothelialization

Human placenta is an attractive source of mesenchymal stem cells (MSC) for regenerative medicine. The cell surface markers expressed on MSC have been proposed as useful tools for the isolation of MSC from other cell populations. However, the correlation between the expression of MSC markers and the ability to support tissue regeneration in vivo has not been well examined. Here, we established several MSC lines from human placenta and examined the expression of their cell surface markers and their ability to differentiate toward mesenchymal cell lineages. We found that the expression of CD349/frizzled‐9, a receptor for Wnt ligands, was positive in placenta‐derived MSC. So, we isolated CD349‐negative and ‐positive fractions from an MSC line and examined how successfully cell engraftment repaired fractured bone and recovered blood flow in ischemic regions using mouse models. CD349‐negative and ‐positive cells displayed a similar expression pattern of cell surface markers and facilitated the repair of fractured bone in transplantation experiments in mice. Interestingly, CD349‐negative, but not CD349‐positive cells, showed significant effects on recovering blood flow following vascular occlusion. We found that induction of PDGFβ and bFGF mRNAs by hypoxia was greater in CD349‐negative cells than in CD349‐positive cells while the expression of VEGF was not significantly different in CD349‐negative and CD349‐positive cells. These findings suggest the possibility that CD349 could be utilized as a specialized marker for MSC isolation for re‐endothelialization. J. Cell. Physiol. 226: 224–235, 2010. © 2010 Wiley‐Liss, Inc.
OptiqueVQS: A visual query system over ontologies for industry

An important application of semantic technologies in industry has been the formalisation of information models using OWL 2 ontologies and the use of RDF for storing and exchanging application data. Moreover, legacy data can be virtualised as RDF using ontologies following the ontology-based data access (OBDA) approach. In all these applications, it is important to provide domain experts with query formulation tools for expressing their information needs in terms of queries over ontologies. In this work, we present such a tool, OptiqueVQS, which is designed based on our experience with OBDA applications in Statoil and Siemens and on best HCI practices for interdisciplinary engineering environments. OptiqueVQS implements a number of unique techniques distinguishing it from analogous query formulation systems. In particular, it exploits ontology projection techniques to enable graph-based navigation over an ontology during query construction. Secondly, while OptiqueVQS is primarily ontology driven, it exploits sampled data to enhance selection of data values for some data attributes. Finally, OptiqueVQS is built on well-grounded requirements, design rationale, and quality attributes. We evaluated OptiqueVQS with both domain experts and casual users and qualitatively compared our system against prominent visual systems for ontology-driven query formulation and exploration of semantic data. OptiqueVQS is available online and can be downloaded together with an example OBDA scenario.
DELAYED REMYELINATION IN RAT SPINAL CORD FOLLOWING ETHIDIUM BROMIDE INJECTION

Areas of demyelination were produced by injecting ethidium bromide into the white matter of the lumbar spinal cord of rats. There was variation in the nature of the process of demyelination and a difference in the speed with which Schwann cells remyelinated the demyelinated axons. In some lesions, or areas within lesions, myelin debris was rapidly processed by macrophages and axons were rapidly remyelinated by Schwann cells, while in other lesions of similar duration, or in areas within the same lesion, the myelin was transformed into lattices of membranous profiles which persisted around axons for long periods of time. In the lesions containing such myelin derived membranes, there were few macrophages and remyelination by Schwann cells was delayed compared to that seen in the more rapidly resolving lesions. It was concluded that the slow resolution of some lesions resulted from the delay between intoxication and cell disintegration (7–10 days) which meant that the cell responses to demyelination took place in a glial free area which could not support cell movement needed for removal of myelin debris and remyelination. This study indicates that the tempo and results of demyelination can be altered by the cellular events which accompany degeneration of oligodendrocytes.
The Hallym Stroke Registry: A Web-Based Stroke Data Bank with an Analysis of 1,654 Consecutive Patients with Acute Stroke

Objectives:In Korea, stroke ranks as one of the leading causes of death along with cancer and ischemic heart disease. The purpose of this study was to better understand clinical characteristics, risk factors and subtypes of acute stroke among Korean people. Method: 1,654 acute stroke patients within 7 days of onset were consecutively enrolled in the Hallym Stroke Registry (HSR). The study was a prospective hospital-based registry aided with a web-based, computerized data bank system. The project began in January 1996 and the data obtained until September 2002 were analyzed. The analysis was basically carried out regarding demographic features, risk factors, time elapsed before coming to the hospitals after the symptom onset, duration of hospitalization, and 30-day mortality rate. Results: 1,567 patients had ischemic stroke and 87 patients had hemorrhagic stroke. The mean age of the patients was 63.6 ± 11.8 years, and 815 patients (56.8%) were men. The estimated time interval between stroke onset and hospital visit was 1.3 days on average and the mean duration of hospital stay was 12.1 days. Only a small fraction (12%) of patients reached the hospital within 3 h after the symptom onset. As for the risk factors, hypertension topped for both ischemic and hemorrhagic strokes followed by smoking and prior history of stroke, diabetes, hyperlipidemia and cardioembolism. With respect to subtype classification of ischemic stroke, large-artery atherosclerosis was most frequent (42.0%), followed by small-vessel occlusion (31.0%), cardioembolism (8.7%), other determined etiology (1.9%), and undetermined etiology (16.4%). The 30-day mortality rate was 7.2%. Conclusion: In the HSR, demographic features were essentially comparable with other major registries. However, the data analysis varies from registry to registry. This may in part be due to a different study design, racial-ethnic differences and patient selection methods.
Benzodiazepine pathways in the chronically ill

The association between patterns of use of benzodiazepines and chronic somatic morbidity was examined by applying the Chronic Disease Score (CDS). In the only pharmacy in a Dutch community, 6921 patients with data available covering a 10‐year period (1983–1992) were included. In 1992, two‐thirds of the patients had a CDS of 0, indicating no chronic morbidity. Patients with chronic morbidity showed a twofold risk of a history of benzodiazepine use. Within the group of benzodiazepine users, longer exposure to benzodiazepines as well as a history of different benzodiazepines were associated with a higher risk of chronic morbidity. Duration of episodes of use and daily dose were not found to be significantly associated with chronic disease. A pattern of increased benzodiazepine use showed a higher risk of chronic morbidity compared with benzodiazepine users with a stable use. Moreover, a history of benzodiazepine use was found to predict a sharp increase in chronic morbidity. We found a clear association between previous and actual use of benzodiazepines and chronic somatic disease. Benzodiazepine use may be induced by sleeping difficulties and anxiety problems caused by the chronic disease. Copyright © 1999 John Wiley & Sons, Ltd.
Induction of cyclooxygenase-2 and activation of nuclear factor-kappaB in myocardium of patients with congestive heart failure.

BACKGROUND Chronic heart failure is associated with induction of secondary inflammatory mediators, including prostanoids. The latter exert diverse functional and morphological effects on cardiac myocytes. Induction of cyclooxygenase (COX), the enzyme responsible for generating prostanoids, requires activation of nuclear factor-kappaB (NF-kappaB). The aim of the present study was to determine the expression of COX-2 and activation of NF-kappaB in the failing human heart. METHODS AND RESULTS Myocardial tissue from 27 patients with end-stage heart failure (various etiologies: ischemic heart disease, n=16; idiopathic dilated cardiomyopathy, n=10; and valvular heart disease, n=1), 2 septic patients, and 8 normal control subjects was immunostained with antisera to COX-2 and NF-kappaB. Western blotting was performed and showed high anti-COX-2 antibody specificity and the presence of COX-2 protein in the sample tissues. In situ hybridization and immunohistochemistry showed little or no expression of COX-2 and NF-kappaB in the control hearts. In contrast, there was abundant expression of COX-2 mRNA and protein in myocytes and inflammatory cells in areas of fibrotic scar compared with regions of normal morphology in all cases of heart failure, except the cases with sepsis, which showed an abundance of COX-2 throughout the myocardium. Sites of NF-kappaB activation were associated with those of COX-2 expression. CONCLUSIONS We demonstrate induction of COX-2 and activation of NF-kappaB in the myocardium of failing human hearts. Induction of both molecules appears to be associated with the presence of inflammation and scar formation.
Particle Filter-Based Estimation of Instantaneous Frequency for the Diagnosis of Electrical Asymmetries in Induction Machines

Fault diagnosis of induction machines operating under variable load conditions is still an unsolved matter. Under those regimes, the application of conventional diagnostic techniques is not suitable, since they are adapted to the analysis of stationary quantities. In this context, modern transient-based methodologies become very appropriate. This paper improves a technique, based on the application of Wigner-Ville distribution as time-frequency decomposition tool, using a particle filtering method as feature extraction procedure, to diagnose and quantify electrical asymmetries in induction machines, such as wound-rotor induction generators used in wind farms. The combination of both tools allows tracking several variable frequency harmonics simultaneously and computing their energy with high accuracy, yielding magnitudes and values similar to those obtained by the application of the fast Fourier transform in stationary operation. The experimental results show the validity of the approach for rapid speed variations, independently of any speed sensor.
Endoglin and alk1 as therapeutic targets for hereditary hemorrhagic telangiectasia

ABSTRACT Introduction: Hereditary Haemorrhagic Telangiectasia (HHT) is as an autosomal dominant trait characterized by frequent nose bleeds, mucocutaneous telangiectases, arteriovenous malformations (AVMs) of the lung, liver and brain, and gastrointestinal bleedings due to telangiectases. HHT is originated by mutations in genes whose encoded proteins are involved in the transforming growth factor β (TGF-β) family signalling of vascular endothelial cells. In spite of the great advances in the diagnosis as well as in the molecular, cellular and animal models of HHT, the current treatments remain just at the palliative level. Areas covered: Pathogenic mutations in genes coding for the TGF-β receptors endoglin (ENG) (HHT1) or the activin receptor-like kinase-1 (ACVRL1 or ALK1) (HHT2), are responsible for more than 80% of patients with HHT. Therefore, ENG and ALK1 are the main potential therapeutic targets for HHT and the focus of this review. The current status of the preclinical and clinical studies, including the anti-angiogenic strategy, have been addressed. Expert opinion: Endoglin and ALK1 are attractive therapeutic targets in HHT. Because haploinsufficiency is the pathogenic mechanism in HHT, several therapeutic approaches able to enhance protein expression and/or function of endoglin and ALK1 are keys to find novel and efficient treatments for the disease.
Adverse events are common on the intensive care unit: results from a structured record review

Intensive care is advanced and highly technical, and it is essential that, despite this, patient care remains safe and of high quality. Adverse events (AEs) are supposed to be reported to internal quality control systems by health‐care providers, but many are never reported. Patients on the intensive care unit (ICU) are at special risk for AEs. Our aim was to identify the incidence and characteristics of AEs in patients who died on the ICUduring a 2‐year period.
Can interpersonal contact help improve attitudes toward soldiers with intellectual disability? An Israeli study

BACKGROUND It has been argued that interpersonal contact is the best intervention for reducing negative attitudes and discrimination toward individuals with intellectual disabilities (ID). In Israel, the Equal in Uniform project makes it possible for individuals with ID to serve alongside soldiers without ID in ordinary military units in the Israeli Defense Forces. The project provided the opportunity to examine the effect of contact on the attitudes of soldiers without ID. METHODS Data analyses are based on data collected from 220 soldiers without ID via a self-administered questionnaire based on the Multidimensional Attitudes Scale. Of these, 138 were in contact with soldiers with ID as part of the project. RESULTS A mediation model showed that the impact of contact with a soldier with ID on withdrawal behaviour was mediated by cognitive attributions and negative affect. Contact was directly associated with prosocial behaviour. DISCUSSION Results support the importance of contact between soldiers with and without ID to bring about improved attitudes toward the former.
Whole blood, red cell and plasma total and ultrafiltrable zinc levels in normal subjects and patients with chronic renal failure with and without haemodialysis.

SUMMARY 1. Plasma and whole-blood zinc levels have been estimated in patients with chronic renal failure with and without haemodialysis with disposable coils and with Kiil artificial kidneys. 2. In the patients not undergoing haemodialysis plasma zinc levels were slightly sub-normal but red cell zinc levels were elevated. 3. In the patients undergoing haemodialysis with disposable coils, the plasma zinc levels were raised before dialysis and rose even further during dialysis. Similar results were found for red cell zinc levels and the additional zinc must have come from the disposable coils. 4. With Kiil dialysis, the pre-dialysis plasma zinc levels were slightly sub-normal, and during dialysis the levels rose much less than with the disposable coils.
Studies on FGF‐2: Nuclear localization and function of high molecular weight forms and receptor binding in the absence of heparin

Multiple forms of FGF‐2 have been shown to exist in many cell types. These different species of molecular masses of 18, 21.5, 22, and 24 kDa are all translated via the use of alternate initiation codons. The three forms of HMW FGF‐2 initiate at CUGs codons, whereas the 18 kDa form initiates at an AUG codon. The entire 18 kDa sequence is contained within the larger forms of HMW FGF‐2 as the AUG codon is 3′ to the CUG codons. Although the 18 kDa form FGF‐2 is localized primarily in the cytosol, a significant fraction of the HMW FGF‐2 has a nuclear location. The nuclear localization of HMW FGF‐2 is determined by amino acid residues in the amino‐terminal extended sequence. The residues required for nuclear localization appear to be RG repeats that are found at multiple sites within the amino‐terminal extension of HMW FGF‐2. The nuclear localization of HMW FGF‐2 suggested that these species may have unique properties. By selecting permanent transfectants of 3T3 cells expressing HMW, 18 kDa FGF‐2, or all forms of FGF‐2, we have found that HMW FGF‐2 can endow cells with a phenotype different from that of cells expressing 18 kDa FGF‐2. These cells are transformed by what appears to be the intracellular action of HMW FGF‐2. The interaction of FGF‐2 with heparin has also been examined. Contrary to other reports claiming that FGF‐2 required heparin or heparan‐sulfate for interaction with its high‐affinity receptor, we have found that FGF‐2 binds to its receptor in the absence of glycosaminoglycans, and that this binding activates the receptor. © 1994 Wiley‐Liss, Inc.
Current Phase II drugs under investigation for the treatment of limb ischemia

Introduction: More than 20 million people in Europe suffer from peripheral arterial disease and nearly 3% develop critical limb ischemia (CLI). Without any medical treatment, CLI has poor prognosis, resulting in limb loss and high mortality rate. Until today, no systemic drug is available for the treatment of CLI and the gold standard method of treatment includes risk factor modification and open surgical or endovascular revascularization. Endovascular local drug delivery devices and novel antithrombotic agents, currently under investigation, aim to improve outcomes of revascularization procedures. The pioneering concept of therapeutic angiogenesis induced by gene and stem cell therapy has been proposed, in an attempt to increase ischemic tissue perfusion. Areas covered: This review summarizes local and systemic pharmacological treatment of CLI using endovascular or pharmaco-biological therapy and focuses on Phase II trials available for these drugs. Expert opinion: Novel endovascular technologies combining angioplasty and local drug-delivery continuously improve and will come to be standard of practice for the management of limb ischemia, while new antithrombotic agents will further improve outcomes. Therapeutic angiogenesis represents a safe and promising treatment option. The combination of revascularization with microcirculation improvement induced by gene or stem cell therapy could enhance limb salvage.
Survival and freedom from aortic valve-related reoperation after valve-sparing aortic root replacement in 1015 patients.

OBJECTIVES The aim of this study was to characterize mortality and aortic valve replacement after valve-sparing aortic root replacement (V-SARR) in a multicentre cohort. METHODS Between 1994 and 2014, 1015 patients had V-SARR with (n = 288, 28%) or without cusp/commissure repair (n = 727, 72%) at the centres of Lübeck (n = 343, 34%), Stuttgart (n = 346, 34%), Hamburg (n = 109, 11%) and Freiburg (n = 217, 21%), Germany. Comparative survival of an age- and gender-matched general population was calculated. Log-rank tests and multiple logistic regression were used to identify risk factors. RESULTS The mean follow-up was 5.2 ± 3.9 years. Cumulative follow-up comprised 2933 patient-years. Early survival was 98%. NYHA status and aneurysm size were predictive of death during mid-term follow-up (P = 0.025). Freedom from aortic valve replacement was 90% at 8 years, with the type of V-SARR (root remodelling, David II) being a risk factor (P = 0.015). Bicuspid aortic valve (P = 0.26) and initial valve function (P = 0.4) did not impact reoperation. The need of additional valve repair (cusps/commissures) was not linked to reoperation: freedom from aortic valve replacement at 8 years was 84% if cusp repair was performed versus 90% if V-SARR alone was performed (P = 0.218). Marfan syndrome had no impact on survival or on aortic valve replacement. CONCLUSIONS Mid-term survival of patients after V-SARR is comparable with that of a matched general population. The regurgitant bicuspid aortic valve is a favourable substrate for V-SARR. Prophylactic surgery should be performed before symptoms or large aneurysms are present to achieve optimal mid-term outcomes.
Plants from the abandoned Nacozari mine tailings: evaluation of their phytostabilization potential

Phytostabilization is a remediation technology that uses plants for in-situ stabilization of contamination in soils and mine tailings. The objective of this study was to identify native plant species with potential for phytostabilization of the abandoned mine tailings in Nacozari, Sonora in northern Mexico. A flora of 42 species in 16 families of angiosperms was recorded on the tailings site and the abundance of the most common perennial species was estimated. Four of the five abundant perennial species showed evidence of regeneration: the ability to reproduce and establish new seedlings. A comparison of selected physicochemical properties of the tailings in vegetated patches with adjacent barren areas suggests that pH, electrical conductivity, texture, and concentration of potentially toxic elements do not limit plant distribution. For the most abundant species, the accumulation factor for most metals was <1, with the exception of Zn in two species. A short-term experiment on adaptation revealed limited evidence for the formation of local ecotypes in Prosopis velutina and Amaranthus watsonii. Overall, the results of this study indicate that five native plant species might have potential for phytostabilization of the Nacozari tailings and that seed could be collected locally to revegetate the site. More broadly, this study provides a methodology that can be used to identify native plants and evaluate their phytostabilization potential for similar mine tailings.
Characteristic Comparison between Five-Leg Inverter and Nine-Switch Inverter

This paper presents a characteristic comparison between a five-leg inverter and a nine-switch inverter. The five-leg inverter and the nine-switch inverter is a single inverter that can drive two AC motors independently. Concretely, we show a circuit architecture of both inverters, a modulation method for an independent drives and a division of voltage utility factor. Moreover, investigate about the inverter losses and a switching device capacity in driving two AC motors independently and adapting a control method to decrease the inverter losses.