Miracle-dz/UniFAR-FTUs huggingface dataset:
Combined Use of the Adenosine A2A Antagonist KW-6002 with 
 l
 -DOPA or with Selective D1 or D2 Dopamine Agonists Increases Antiparkinsonian Activity but Not Dyskinesia in MPTP-Treated Monkeys

The novel selective adenosine A(2A) receptor antagonist KW-6002 improves motor disability in MPTP-treated parkinsonian marmosets without provoking dyskinesia. In this study we have investigated whether KW-6002 in combination with l-DOPA or selective D1 or D2 dopamine receptor agonists enhances antiparkinsonian activity in MPTP-treated common marmosets. Combination of KW-6002 with the selective dopamine D2 receptor agonist quinpirole or the D1 receptor agonist SKF80723 produced an additive improvement in motor disability. Coadministration of KW-6002 with a low dose of L-DOPA also produced an additive improvement in motor disability, and increased locomotor activity. The ability of KW-6002 to enhance antiparkinsonian activity was more marked with L-DOPA and quinpirole than with the D1 agonist. However, despite producing an enhanced antiparkinsonian response KW-6002 did not exacerbate L-DOPA-induced dyskinesia in MPTP-treated common marmosets previously primed to exhibit dyskinesia by prior exposure to L-DOPA. Selective adenosine A(2A) receptor antagonists, such as KW-6002, may be one means of reducing the dosage of L-DOPA used in treating Parkinson's disease and are potentially a novel approach to treating the illness both as monotherapy and in combination with dopaminergic drugs.
Induction of Parturition in the Cow using Cloprostenol and Dexamethasone in Combination.

Two experiments were designed to test the hypothesis that induction of parturition in the cow would be more predictable with the simultaneous use of a combination of cloprostenol and dexamethasone than with either hormone used alone.In experiment I all 19 beef cows treated with 500 mug cloprostenol and 25 mg dexamethasone in combination calved within 72 hours whereas dexamethasone (n = 19) or cloprostenol (n = 16) treatments alone each resulted in two induction failures. In those cows successfully induced, the mean interval from treatment to birth was 34.6 +/- 1.4 hours for the cloprostenol plus dexamethasone group, 43.3 +/- 2.4 hours for the dexamethasone group and 44.9 +/- 2.1 hours for the cloprostenol group. Control cows (n = 15) did not calve during the first 72 hours after treatment with saline. The incidence of retained placenta ranged from 19 to 53% in induced groups whereas placentae were not retained by cows in the control group.In experiment II all 30 beef cows in the cloprostenol plus dexamethasone group calved within the 72 hour limit, with a mean interval of 39.1 +/- 1.0 hours. Twenty-six of 31 cows calved within 72 hours with a mean interval of 51.9 +/- 3.4 hours after a single injection of cloprostenol and 29 of 33 cows calved within 72 hours with a mean interval of 52.6 +/- 3.3 hours after two injections of cloprostenol, 12 hours apart. Five of 34 control cows calved within 72 hours of time of treatment. The incidence of retained placenta was again high in induced cows. Results indicate that the simultaneous administration of cloprostenol and dexamethasone does constitute a safe, reliable and effective method of inducing parturition in the cow.
Structure of complex of synthetic HIV-1 protease with a substrate-based inhibitor at 2.3 A resolution.

The structure of a complex between a peptide inhibitor with the sequence N-acetyl-Thr-Ile-Nle-psi[CH2-NH]-Nle-Gln-Arg.amide (Nle, norleucine) with chemically synthesized HIV-1 (human immunodeficiency virus 1) protease was determined at 2.3 A resolution (R factor of 0.176). Despite the symmetric nature of the unliganded enzyme, the asymmetric inhibitor lies in a single orientation and makes extensive interactions at the interface between the two subunits of the homodimeric protein. Compared with the unliganded enzyme, the protein molecule underwent substantial changes, particularly in an extended region corresponding to the "flaps" (residues 35 to 57 in each chain), where backbone movements as large as 7 A are observed.
Changes in the Competitive Advantages of Croatia's Manufacturing Industry

The aim of this research is to analyse the position and detecte competitive advantages of Croatia’s manufacturing industry in foreign exchange trading. The methodology of this research is based on the application of indices of openness to foreign trade and detected competitive advantages, as well as the complete analysis of the manufacturing industry structure of the Republic of Croatia. In order to analyse the competitive advantages of the manufacturing industry in the Republic of Croatia the following quantitative methods were used: Revealed Comparative Advantage (RCA), as a method for detecting comparative advantages of exporting goods of some countries, Export Competitiveness Indices (XS), as a method for measuring export competitiveness of the observed countries, Export Specialization Index (ES), as a method for comparing export activities of the manufacturing industry in the Republic of Croatia and the European Union, and the Relative Trade Advantage Index (RTA) that incorporates the RCA and the RMA indices. The main results of the research indicate competitive advantages detected by measuring, evaluation of the Croatian manufacturing industry particularly on foreign markets, recommendations and proposals in order to reach a higher level of export competitiveness. The main conclusion of this research is that there is a need to establish prerequisites and to define measures for structural changes in the Croatian manufacturing industry.
‘Blindness to the obvious’? Treatment experiences and feminist approaches to eating disorders

Eating disorders are currently often approached as biopsychosocial problems. But the social or cultural aspects of the equation are frequently marginalised in treatment – relegated to mere contributory or facilitating factors. In contrast, feminist and socio-cultural approaches are primarily concerned with the relationship between eating disorders and the social/cultural construction of gender. Yet, although such approaches emerged directly from the work of feminist therapists, the feminist scholarship has increasingly observed, critiqued and challenged the biomedical model from a scholarly distance. As such, this article draws upon data from 15 semi-structured interviews with women in the UK context who have experience of anorexia and/or bulimia in order to explore a series of interlocking themes concerning the relationship between gender identity and treatment. In engaging the women in debate about the feminist approaches (something that has been absent from previous feminist work), the article examines how gender featured in women’s understandings of their problem, and the ways in which it was – or was not – addressed in treatment. The article also explores the women’s evaluations of the feminist discourse, and their discussions of how it might be implemented within therapeutic and clinical contexts.
Aortic valve replacement with the Lillehei-Kaster prosthesis in 262 patients: an assessment after 9 to 17 years.

A 9 to 17-year follow-up after aortic valve replacement with the Lillehei-Kaster prosthesis was carried out in 262 patients with a mean age of 53 years. All patients were traced. The operative mortality was 7.3%. The observed survival of the total series after 5, 10 and 15 years amounted to 75%, 61%, and 49% respectively. The majority of survivors showed improvement of functional capacity and decrease of heart volume. Freedom from valve-related death, thromboembolism and valve-related morbidity and mortality after 15 years amounted to 94%, 85% and 75% respectively. Only two instances of valve thrombosis were seen, and fracture of the prosthesis was never encountered. These results compare favourably with those obtained with the Starr-Edwards and the Björk-Shiley prostheses. Analysis of the relative survival rates, i.e. the observed survival rates as ratios of those of the general population, indicates that the 10-year survival of patients with aortic stenosis is close to normal, whereas the survival rates of patients with aortic incompetence and with combined aortic stenosis and incompetence are significantly lower. It is suggested that earlier operation should be considered in patients with aortic incompetence and with combined stenosis and incompetence in order to lower the late mortality rate.
A Coupled Atmosphere–Ocean GCM Study of the ENSO Cycle

Abstract This study examines interannual variability produced by a recent version of the University of California, Los Angeles, coupled atmosphere–ocean general circulation model (CGCM). The CGCM is shown to produce ENSO-like climate variability with reasonable frequency and amplitude. A multichannel singular spectrum analysis identifies the simulated ENSO cycle and permits examination of the associated evolution of atmospheric and oceanic states. During the cycle, the evolution of upper-ocean heat content in the tropical Pacific is characterized by a zonal oscillation between the western and eastern equatorial Pacific and a meridional oscillation between the equator and 10°N. The zonal oscillation is related to the amplification of the cycle, and the meridional oscillation is related to the transition between phases of the cycle. It is found that the north–south ocean heat content difference always reaches a threshold near the onset of a warm/cold event. The three-dimensional evolution of ocean temperatu.
Resistance to the photosystem II herbicide diuron is dominant to sensitivity in the cyanobacterium Synechococcus sp. PCC7942

The transformable cyanobacterium, Synechococcus sp. PCC7942, was used to study the genetics of resistance to the herbicide diuron. In wild‐type cells, diuron binds to one of the core proteins, called D1, of photosystem II reaction centres. This binding prevents the transfer of electrons from QA, the primary quinone acceptor, to QB, which is necessary to create the charge separation that drives ATP synthesis. A single amino acid substitution in the D1 protein reduces diuron binding and confers herbicide resistance to reaction centres containing the substituted D1 protein. In Synechococcus 7942, the D1 protein is encoded by three functional genes called psbAI, psbAII and psbAIII. By selectively altering one member at a time of the three‐member psbA gene family, we have demonstrated that diuron‐resistant alleles are dominant to diuron‐sensitive alleles. The relative abundance of the different psbA gene transcripts is correlated with the fraction of diuron‐resistant reaction centres and with the degree of diuron resistance. Growth in sublethal diuron selectively increases the steady‐state levels of transcripts of genes (psbA and psbD) encoding the core proteins of photosystem II. We have also found that turnover of the D1 protein can be uncoupled from electron transport through photosystem II.
Understanding the consequences of victory amongst sport spectators: The mediating role of BIRGing

Abstract Prior work on the relationships between team identification and spectators’ reactions to one's team victory has largely neglected the potential effects of mediating variables. In this research, we proposed that the process of Basking in Reflected Glory [BIRGing – the tendency to reduce the distance between oneself and one's team; Cialdini, R. B. , Bordon, R. J., Thorne, A. , Walker, M. R. , Freeman, S. , & Sloan, L. R. (1976). Basking in reflected glory: Three (football) field studies. Journal of Personality and Social Psychology, 34, 366–375] would lead spectators to almost take ownership of victories and share its benefits, thereby impacting their sense of national belonging in a positive manner. To this end, participants (N = 73) were asked to watch an edited video clip showing a victory of the French national rugby team playing against South Africa. As expected, results revealed that BIRGing mediated the relationship between team identification and one's sense of national belonging. In closing, a number of implications are discussed along with future research avenues pertaining to the sport spectatorship literature.
Clinical course and visual function in a family with mutations in the RPE65 gene.

OBJECTIVE To evaluate the phenotype of affected and carrier members of a family with mutations in RPE65 (a retinal pigment epithelium gene). METHODS RPE65 mutation screening was performed on DNA from 2 affected brothers, 1 unaffected brother, both parents, and 3 surviving grandparents using cycle sequencing. Ophthalmic examinations included ophthalmoscopic fundus examination; visual function testing; 2-color, static, dark-adapted threshold perimetry; and rod electroretinographic a-wave phototransduction analysis. RESULTS The 2 affected brothers carried RPE65 mutations in compound heterozygous form: a maternal Y368H (1156T-->C) missense mutation and a paternal IVS1 + 5g-->a splice-site mutation. Severe visual deficits and an absence of rod and cone electroretinographic responses were diagnosed in both affected boys before the age of 5 years. Visual acuities of about 20/100 during grade school declined to hand movements by the teenage years, and only a rudimentary peripheral temporal visual field remained by the ages of 25 and 29 years. Both parents had normal central visual function, as measured by visual acuity, contrast sensitivity, color vision, and Humphrey 10-2 fields. However, the 50-year-old father showed hundreds of tiny whitish hard drusen in both eyes and had abnormal peripheral function on dark-adapted perimetry, with extended field defects of 15 to 20 dB outside 30 degrees eccentricity. His rod photoreceptor sensitivity and amplitude, calculated by fitting the rod a waves by a model of activation of phototransduction, were normal, but the flicker electroretinographic response was delayed. CONCLUSIONS The RPE65 mutations Y368H and IVS1 + 5g-->a present in compound heterozygous form cause severe visual compromise in childhood and progress to nearly total vision loss by the second to third decades of life. The retinal and functional changes in the father carrying a presumed functional null allele suggest that some RPE65 heterozygous carriers may manifest visual symptoms.
A Conserved Domain of Schizosaccharomyces pombe dfp1+ Is Uniquely Required for Chromosome Stability following Alkylation Damage during S Phase

ABSTRACT The fission yeast Dbf4 homologue Dfp1 has a well-characterized role in regulating the initiation of DNA replication. Sequence analysis of Dfp1 homologues reveals three highly conserved regions, referred to as motifs N, M, and C. To determine the roles of these conserved regions in Dfp1 function, we have generated dfp1 alleles with mutations in these regions. Mutations in motif N render cells sensitive to a broad range of DNA-damaging agents and replication inhibitors, yet these mutant proteins are efficient activators of Hsk1 kinase in vitro. In contrast, mutations in motif C confer sensitivity to the alkylating agent methyl methanesulfonate (MMS) but, surprisingly, not to UV, ionizing radiation, or hydroxyurea. Motif C mutants are poor activators of Hsk1 in vitro but can fulfill the essential function(s) of Dfp1 in vivo. Strains carrying dfp1 motif C mutants have an intact mitotic and intra-S-phase checkpoint, and epistasis analysis indicates that dfp1 motif C mutants function outside of the known MMS damage repair pathways, suggesting that the observed MMS sensitivity is due to defects in recovery from DNA damage. The motif C mutants are most sensitive to MMS during S phase and are partially suppressed by deletion of the S-phase checkpoint kinase cds1. Following treatment with MMS, dfp1 motif C mutants exhibit nuclear fragmentation, chromosome instability, precocious recombination, and persistent checkpoint activation. We propose that Dfp1 plays at least two genetically separable roles in the DNA damage response in addition to its well-characterized role in the initiation of DNA replication and that motif C plays a critical role in the response to alkylation damage, perhaps by restarting or stabilizing stalled replication forks.
Induction of hair growth by skin irritants and its relation to skin protein kinase C isoforms

Induction of hair growth by skin irritants and its relation to skin protein kinase C (PKC) isoforms were evaluated. Dorsal skin of BALB/c mice was shaved and anthralin (0.1% in corn oil) was applied on one side of the shaved backs in 20 mice daily for 5 days. The corresponding opposite side treated with corn oil served as a control. In another 20 mice, sodium dodecyl sulphate (SDS, 10% in water) was applied on one side of the shaved backs for 5 days by the same procedure as above and the corresponding opposite side treated with water served as control. Visible acceleration of hair growth on anthralin‐treated skin was observed as early as 14 days after the application of anthralin and significant hair growth was observed at about 17–20 days. Enhancement of hair growth on SDS‐treated skin was observed at about 3 weeks from the beginning of the treatment. None of the mice showed remarkable hair growth on the control side in either group. Mouse skin PKC isoforms levels detected by Western blot showed a similar pattern in both treatment groups. PKC α was downregulated initially, and was then elevated from 3 days after anthralin treatment and 14 days after SDS application. PKC β was unchanged initially, decreased at 8 and 14 days after anthralin and SDS treatment, respectively, and reverted to the control level at 25 days after anthralin treatment, when it was still lower than the control in SDS‐treated skin. PKC δ was also unchanged at first, but was elevated from 3 days after anthralin treatment and 14 days after SDS application. These results suggest that involvement of PKC may be a general phenomenon in irritant‐induced hair growth in mice. Considering the stimulatory effect of PKC α and inhibitory effect of PKC δ on cell growth, we postulate that PKC α may be responsible for enhancement of hair growth while PKC δ may inhibit hair growth to keep the hair growth in balance.
Quality of Life before and after Lung Transplantation in Patients with Emphysema versus other Indications

Whether lung transplantation improves Health-related Quality of Life in patients with emphysema and other end-stage lung diseases before and after lung transplantation was examined. Berween 1992 and 1999, 23 patients with emphysema and 19 patients with other indications completed self-administered questionnaires before lung transplantation, and at 4, 7, 13, and 25 mo. after transplantation. The questionnaire included the Nottingham Health Profile, the State-Trait Anxiety Inventory, the Self-rating Depression Scale, the Index of Well-being, the self-report Karnofsky Index, and four respiratory-specific questions. Neither before nor after transplantation were significant differences found on most dimensions of Health-related Quality of Life between patients with emphysema and other indications. Before transplantation, both groups report major restrictions on the dimensions Energy and Mobility of the Nottingham Health Profile, low experienced well-being, depressive symptoms, and high dyspnea. About 4 mo. after transplantation, most Health-related Quality of Life measures improved significantly in both groups. These improvements were maintained in the following 21 mo.
Entorhinal cortex pathology in Alzheimer's disease

The anatomical distribution of pathological changes in Alzheimer's disease, although highly selective for only certain brain areas, can be widespread at the endstage of the illness and can affect many neural systems. Propriety for onset among these is a question of importance for clues to the etiology of the disease, but one that is formidable without an experimental animal model. The entorhinal cortex (Brodmann's area 28) of the ventromedial temporal lobe is an invariant focus of pathology in all cases of Alzheimer's disease with selective changes that alter some layers more than others. The authors'findings reveal that it is the most heavily damaged cortex in Alzheimer's disease. Neuroanatomical studies in higher mammals reveal that the entorhinal cortex gives rise to axons that interconnect the hippocampal formation bidirectionally with the rest of the cortex. Their destruction in Alzheimer's disease could play a prominent role in the memory deficits that herald the onset of Alzheimer's disease and that characterize it throughout its course.
Towards Moment Imagery: Automatic Cinemagraphs

The imagination of the online photographic community has recently been sparked by so-called cinema graphs: short, seamlessly looping animated GIF images created from video in which only parts of the image move. These cinema graphs capture the dynamics of one particular region in an image for dramatic effect, and provide the creator with control over what part of a moment to capture. We create a cinema graphs authoring tool combining video motion stabilisation, segmentation, interactive motion selection, motion loop detection and selection, and cinema graph rendering. Our work pushes toward the easy and versatile creation of moments that cannot be represented with still imagery.
Subunit structure of cholera toxin.

SUMMARY Two types of subunit, with molecular weights estimated to be 28000 and 8000, were demonstrated in the cholera exo-enterotoxin by sodium dodecylsulphate polyacrylamide electrophoresis. The light (L) component but not the heavy (H) was demonstrable in the antigenically identical natural toxoid. A split of the H component by reduction and alkylation suggested it to be a disulphide-bridged dimer. Gel filtration at acid pH permitted separation of the H and L subunits in isolated form. Fractions with isolated subunits were non-toxic (skin test), in contrast to fractions with intermediate filtration rate containing both subunits. Fractions with a L: H ratio of about 3.5 were even more toxic than the intact toxin (L: H ratio about 2). Addition of the isolated subunits to toxic fractions generally caused inhibition and only exceptionally potentiation of toxicity. The data suggest that the cholera toxin might consist of an aggregate of seven L and one H subunits while the natural toxoid seems composed of only the aggregated L chains.