Miracle-dz/UniFAR-FTUs huggingface dataset:
Behavior of a Short preS1 Epitope on the Surface of Hepatitis B Core Particles

Abstract The major immunodominant region of hepatitis B core particles is widely recognized as the most prospective target for the insertion of foreign epitopes, ensuring their maximal antigenicity and immunogenicity. This region was mapped around amino acid residues 79–81, which were shown by electron cryo-microscopy to be located on the tips of the spikes protruding from the surface of hepatitis B core shells. Here we tried to expose a model sequence, the short immunodominant hepatitis B preS1 epitope 31-DPAFR-35, onto the tip of the spike, with simultaneous deletion of varying stretches from the major immunodominant region of the HBc molecule. Accessibility to the monoclonal anti-preS1 antibody MA18/7 and specific immunogenicity of the preS1 epitope depended on the location and length of the deletion. While chimeras with deletions within the stretch 79–88 presented the preS1 epitope on their surface and demonstrated remarkable preS1 immunogenicity, the corresponding chimeras without any deletion or with a more prolonged deletion (79–93) were unable to provide such presentation and possessed a lower specific preS1 immunogenicity. Deletion of the stretch 79–81 was sufficient to avoid the intrinsic HBc immunogenicity of the core particles, although chimeras with deleted major immunodominant region retained their property to be recognized by human polyclonal or hyperimmune polyclonal or hyperimmune anti-HBc antibodies
The Role of Capital Realignment Versus In Situ Stabilization for the Treatment of Slipped Capital Femoral Epiphysis

Introduction: Slipped capital femoral epiphysis (SCFE) can be treated by a variety of methods with the traditional method of in situ pin fixation being most commonly used. More recently, the Modified Dunn (Mod. Dunn) procedure consisting of capital realignment has been popularized as a treatment method for SCFE, particularly for more severe cases. Over the last 5 years, our institution has selectively used this method for more complex cases. The purpose of this article is to evaluate the differences between these 2 treatment methods in terms of avascular necrosis (AVN) rate, reoperation rate, and complication rate. Methods: Eighty-eight hips that were surgically treated for SCFE between July 2004 and June 2012 met our inclusion criteria. The in situ fixation group included 71 hips, whereas 17 hips were anatomically reduced with the Mod. Dunn procedure. Loder classification, severity, acuity, complication rate, and reoperation rate were determined for the 2 cohorts. The &khgr;2 analysis was performed to evaluate the relationship between the treatment method and outcome. Results: As expected, stable slips did well with in situ pinning with no cases of AVN, even in more severe slips. Ten stable slips were treated with the Mod. Dunn approach and 2 (20%) developed AVN. Unstable slips were more difficult to treat with 3 of the 7 hips stabilized in situ developing AVN (43%). Two of the 7 unstable slips treated by the Mod. Dunn procedure developed AVN (29%). The other outcomes studied (reoperation rate and complication rate) were not significantly related to the surgical treatment method (P=0.732 and 0.261, respectively). Conclusions: In situ pinning remains a safe and predictable method for treatment of stable SCFE with no AVN noted, even in severe slips. Attempts to anatomically reduce stable slips led to severe AVN in 20% of cases, thus this treatment approach should be considered with caution. Treatment of unstable slips remains problematic with high AVN rates noted whether treated by in situ fixation or capital realignment (Mod. Dunn). Level of Evidence: Level III retrospective comparative study.
A novel HIV-CCR5 receptor vaccine strategy in the control of mucosal SIV/HIV infection

Objective: To develop a novel SIV-CCR5 receptor vaccine strategy that will protect macaques from SHIV infection by the vaginal mucosal route. Design: The rationale for this strategy is that humans who express the homozygous Δ32 CCR5 mutation and the associated upregulation of CC chemokines, the downmodulation of cell-surface expression of CCR5 and antibodies to CCR5 are protected against HIV infection. Methods: A vaccine was prepared consisting of three extracellular peptides of CCR5, an N-terminal HIV gp120 fragment generated in transgenic plants and recombinant SIV p27. These were linked to the 70 000 Mr microbial heat shock protein (HSP70) carrier. The vaccine was administered (×3) either by the vaginal mucosal route or by targeting the proximity of the draining iliac lymph nodes. Results: Serum and vaginal fluid IgG and IgA antibodies, IL-2 and IFN-γ-producing cells, and macrophage-inflammatory protein (MIP) 1β and MIP-1α (CCL4 and CCL3) were significantly raised in immunized macaques (P = 0.01–0.05). Vaginal challenge with SHIV89.6P infected all macaques, but sequential analysis over 24 weeks showed a significant variation in viral loads between the animals (P = 0.05). Whereas SHIV89.6P persisted in the four unimmunized macaques, in five of the eight immunized macaques the virus was cleared or became undetectable by reverse transcriptase–polymerase chain reaction. The CD4 cell counts in the immunized macaques were significantly higher than those in unimmunized animals (P < 0.05). Conclusion: An immunization strategy that targets both the virus and its CCR5 receptor has significantly inhibited SHIV89.6P infection and may serve as a novel strategy in the prevention of HIV transmission.
Disaggregated Credit, Liquidity and Housing Price Dynamics

When disentangling the real effects of bank credit in the demand-supply dynamics of both the asset market and the real economy, it is disaggregate bank credit, which holds quantitatively important information. This is all the more relevant when persistent economic policy uncertainty dominates the demand-supply equilibrium in these markets. To examine this in the context of the international housing market, we develop an empirically testable framework and perform a panel VAR estimation for a unique quarterly dataset consisting of nine industrialized countries. We find that credit to the real economy and housing prices depict mutually reinforcing relationships. Moreover, we find that there is a negligible negative effect of credit to the asset market on housing prices in the short-run, and such effects are positive over the long-run. Alternatively, the effect of housing prices on credit to the asset market is positive in both the shortand long-run. Moreover, the dynamic interdependent effects of housing prices and credit to the asset market are more pronounced when economic policy uncertainty and the global financial crisis are respectively accounted for in our estimation. Finally, a battery of robustness checks confirms our predictions.
Pancreatic adenocarcinoma: Regression analysis to identify improved cytologic criteria

The incidence of pancreatic adenocarcinoma is increasing and it is usually unresectable at the time of diagnosis. Consequently, fine‐needle aspiration biopsy (FNAB) is being used more frequently for diagnosis. The reported sensitivity of diagnosing pancreatic adenocarcinoma by FNAB has varied between 50% and 100%. In an attempt to increase the diagnostic sensitivity, we retrospectively reviewed a series of pancreatic FNABs. Fifteen cytologic criteria were evaluated in 78 patients who had pancreatic FNABs. Of these patients, 49 had primary adenocarcinomas and 29 had benign, non‐neoplastic lesions. Using a stepwise logistic regression analysis we identifed three key cytologic criteria for this diagnosis. Our study identifed anisonucleosis (P = 0.001). large nuclei (P = .007), and nuclear molding (P = .03) as the significant cytologic features for diagnosing pancreatic adenocarcinoma. In combination, these three criteria had a sensitivity of 98% and a specificity of 100%.
The Mediating Role of Ethical Climate in the Relationship Between Performance Appraisal and Organizational Performance

ABSTRACT Although prior research has consistently established a positive relationship between performance appraisal and organizational performance across different research contexts, the mechanisms that underlie this link remain unclear. The present study attempts to fill this void by testing the mediating role of ethical climate in the relationship between performance appraisal and organizational performance across 181 Nigerian public sector agencies. Cross-sectional mediation analyses were performed using partial least squares path modeling approach. Results indicated that performance appraisal was positively and significantly related to organizational performance. Regarding the mediating effect, ethical climate significantly mediated the relationship between performance appraisal and organizational performance. The study made recommendations and drew suggestions for future research to enhance the performance of public sector agencies.
Identification with External Instruments in Structural Vars Under Partial Invertibility

This paper discusses the conditions for indentification with external instruments in Structural VARs under partial invertibility. We observe that in this case the shocks of interest and their effects can be recovered using an external instrument, provided that a condition of limited lag exogeneity holds. This condition is weaker than that required for LP-IV, and allows for recoverability of impact effects also une VAR misspecification. We assess our claims in a simulated environment, and provide an emirical application to the relevant cas of identification of monetary policy shocks.
Estimation of hydrological response to future climate change in a cold watershed

The vulnerability of the natural water system in cold areas to future climate change is of great concern. A coupled model approach was applied in the headwater watershed area of Yalu River in the northeastern part of China to estimate the response of hydrological processes to future climate change with moderate data. The stochastic Long Ashton Research Station Weather Generator was used to downscale the results of general circulation models to generate synthetic daily weather series in the 2050s and 2080s under various projected scenarios, which were applied as input data of the Generalized Watershed Loading Functions hydrological model for future hydrological process estimations. The results showed that future wetter and hotter weather conditions would have positive impacts on the watershed runoff yields but negative impacts on the watershed groundwater flow yields. The freezing period in winter would be shortened with earlier snowmelt peaks in spring. These would result in less snow cover in winter and shift the monthly allocations of streamflow with more yields in March but less in April and May, which should be of great concern for future local management. The proposed approach of the coupled model application is effective and can be used in other similar areas.
From Contrary to Complementary Models: Central Places and Gateways in the South-Eastern Provence (Arles and Marseille)

This paper applies the concepts of gateways and centrality, formerly opposing approaches to spatial planning, by now a powerful merged tool for archaeologists, to understand the dynamics of the evolution of cities and settlements in a long-term perspective. The samples are the two main port cities in South-Eastern Provence (France), Marseille and Arles. By means of several archaeological markers it will be shown how natural landscapes and political control influenced the fate of the economic development of both cities in Greco-Roman times. Therefore, this study focuses on the aspects of trade and administration encompassing the functionality of the ports as trans-shipment centers, the impact of political interference as well as the supply and exchange of long distance and local/regional products. Within this research framework, Marseille emerged as a static gateway for its service area with a distinct perspective on Mediterranean trade. Arles, however, was the main gateway for the whole Rhône corridor in Roman times due to its strategic location in an area characterized by a variety of landscapes and the promotion of politics as a port of the annona. The data presented here aim to reject the frequently used narrative of an ongoing competition between Arles and Marseille in favor of a more nuanced picture of economic interactions and overlapping trading networks.
Cisternostomy: Replacing the age old decompressive hemicraniectomy?

Backround: Practical scenario in trauma neurosurgery comes with multiple challenges and limitations. It accounts for the maximum mortality in neurosurgery and yet the developing countries are still ill-equipped even for an emergency set-up for primary management of traumatic brain injuries. The evolution of modern neurosurgical techniques in traumatic brain injury has been ongoing for the last two centuries. However, it has always been a challenge to obtain a satisfactory clinical outcome, especially those following severe traumatic brain injuries. Other than the well-established procedures such as decompressive hemicraniectomy and those for acute and or chronic subdural hematomas and depressed skull fractures, contusions etcetera newer avenues for development of surgical techniques where indicated have been minimal. We are advocating a replacement for decompressive hemicranictomy, which would have the same indications as decompressive hemicraniectomy. The results of this procedure has been compared with the results of decompressive hemicraniectomy done in our institution and elsewhere and has been proven beyond doubts to be superior to decompressive hemicraniectomy. This procedure is elegant and can replace decompressive hemicraniectomy because of low morbidity and mortality. However, there is a steep learning curve and the microscope has to be used. Materials and Methods: Based on the clinical experience and observation of acute neurosurgical service in tertiary medical centers in a developing country, the procedure of cisternostomy in the management of trauma neurosurgery have been elucidated in the current study. The study proposes to apply the principles of microvascular surgery and skull base surgery in selected cases of severe traumatic brain injuries, thus replacing decompressive hemicraniectomy as the primary modality of treatment for indicated cases. Conclusion: Extensive opening of cisterns making use of skull base techniques to approach them in a swollen brain is a better option to decompressive hemicraniectomy for the same indications.
Alert signals enhance animal communication in “noisy” environments

Environmental noise that reduces the probability that animals will detect communicative signals poses a special challenge for long-range communication. The application of signal-detection theory to animal communication lead to the prediction that signals directed at distant receivers in noisy environments will begin with conspicuous “alerting” components to attract the attention of receivers, before delivery of the information-rich portion of the signal. Whether animals actually adopt this strategy is not clear, despite suggestions that alerts might exist in a variety of taxa. By using a combination of behavioral observations and experimental manipulations with robotic lizard “playbacks,” we show that free-living territorial Anolis lizards add an “alert” to visual displays when communicating to distant receivers in situations of poor visibility, and that these introductory alerts in turn enhance signal detection in adverse signaling conditions. Our results show that Anolis lizards are able to evaluate environmental conditions that affect the degradation of long-distance signals and adjust their behavior accordingly. This study demonstrates that free-living animals enhance the efficiency of long-range communication through the modulation of signal design and the facultative addition of an alert. Our findings confirm that alert signals are an important strategy for communicating in “noisy” conditions and suggest a reexamination of the existence of alerts in other animals relying on long-range communication.
The effect of lower body cooling on the changes in three core temperature indices

Rectal (Tre), ear canal (Tear) and esophageal (Tes) temperatures have been used in the literature as core temperature indices in humans. The aim of the study was to investigate if localized lower body cooling would have a different effect on each of these measurements. We hypothesized that prolonged lower body surface cooling will result in a localized cooling effect for the rectal temperature not reflected in the other core measurement sites. Twelve participants (mean ± SD; 26.8 ± 6.0 years; 82.6 ± 13.9 kg; 179 ± 10 cm, BSA = 2.00 ± 0.21 m2) attended one experimental session consisting of sitting on a rubberized raft floor surface suspended in 5 °C water in a thermoneutral air environment (∼21.5 ± 0.5 °C). Experimental conditions were (a) a baseline phase during which participants were seated for 15 min in an upright position on an insulated pad (1.408 K ⋅ m2 ⋅ W−1); (b) a cooling phase during which participants were exposed to the cooling surface for 2 h, and (c) an insulation phase during which the baseline condition was repeated for 1 h. Temperature data were collected at 1 Hz, reduced to 1 min averages, and transformed from absolute values to a change in temperature from baseline (15 min average). Metabolic data were collected breath-by-breath and integrated over the same temperature epoch. Within the baseline phase no significant change was found between the three indices of core temperature. By the end of the cooling phase, Tre was significantly lower (Δ = −1.0 ± 0.4 °C) from baseline values than from Tear (Δ = −0.3 ± 0.3 °C) and Tes (Δ = −0.1 ± 0.3 °C). Tre continued to decrease during the insulation phase from Δ −1.0 ± 0.4 °C to as low as Δ −1.4 ± 0.5 °C. By the end of the insulation phase Tre had slightly risen back to Δ −1.3 ± 0.4 °C but remained significantly different from baseline values and from the other two core measures. Metabolic data showed no variation throughout the experiment. In conclusion, the local cooling of the buttock area results in a drop in rectal temperature compromising the validity of the rectal temperature as a core temperature index under these conditions.
Mechanism of Aurora-B Degradation and Its Dependency on Intact KEN and A-Boxes: Identification of an Aneuploidy-Promoting Property

ABSTRACT The kinase Aurora-B, a regulator of chromosome segregation and cytokinesis, is highly expressed in a variety of tumors. During the cell cycle, the level of this protein is tightly controlled, and its deregulated abundance is suspected to contribute to aneuploidy. Here, we provide evidence that Aurora-B is a short-lived protein degraded by the proteasome via the anaphase-promoting cyclosome complex (APC/c) pathway. Aurora-B interacts with the APC/c through the Cdc27 subunit, Aurora-B is ubiquitinated, and its level is increased upon treatment with inhibitors of the proteasome. Aurora-B binds in vivo to the degradation-targeting proteins Cdh1 and Cdc20, the overexpression of which accelerates Aurora-B degradation. Using deletions or point mutations of the five putative degradation signals in Aurora-B, we show that degradation of this protein does not depend on its D-boxes (RXXL), but it does require intact KEN boxes and A-boxes (QRVL) located within the first 65 amino acids. Cells transfected with wild-type or A-box-mutated or KEN box-mutated Aurora-B fused to green fluorescent protein display the protein localized to the chromosomes and then to the midzone during mitosis, but the mutated forms are detected at greater intensities. Hence, we identified the degradation pathway for Aurora-B as well as critical regions for its clearance. Intriguingly, overexpression of a stable form of Aurora-B alone induces aneuploidy and anchorage-independent growth.
Monitoring angiogenesis using a human compatible calibration for broadband near-infrared spectroscopy

Abstract. Angiogenesis is a hallmark of many conditions, including cancer, stroke, vascular disease, diabetes, and high-altitude exposure. We have previously shown that one can study angiogenesis in animal models by using total hemoglobin (tHb) as a marker of cerebral blood volume (CBV), measured using broadband near-infrared spectroscopy (bNIRS). However, the method was not suitable for patients as global anoxia was used for the calibration. Here we determine if angiogenesis could be detected using a calibration method that could be applied to patients. CBV, as a marker of angiogenesis, is quantified in a rat cortex before and after hypoxia acclimation. Rats are acclimated at 370-mmHg pressure for three weeks, while rats in the control group are housed under the same conditions, but under normal pressure. CBV increased in each animal in the acclimation group. The mean CBV (%volume/volume) is 3.49%±0.43% (mean±SD) before acclimation for the experimental group, and 4.76%±0.29% after acclimation. The CBV for the control group is 3.28%±0.75%, and 3.09%±0.48% for the two measurements. This demonstrates that angiogenesis can be monitored noninvasively over time using a bNIRS system with a calibration method that is compatible with human use and less stressful for studies using animals.