Miracle-dz/UniFAR-FTUs huggingface dataset:
Existential plight of adult daughters following their mother's breast cancer diagnosis

Daughters caring for a mother with breast cancer represent a vulnerable population. They are confronted with having to integrate their emotional reactions to their mother's illness while simultaneously processing concerns about their own personal susceptibility. Through their caregiving, daughters obtain intimate knowledge of their mother's breast cancer experience. As part of a study of breast cancer survivorship and the family, in‐depth interviews were conducted with a diverse sample of adult daughters caring for their mother with breast cancer. Analysis of the daughters' narratives documents that their mother's cancer diagnosis subjects them to a period of crisis fraught with severe emotional distress and life and death concerns that parallels the ‘existential plight’ that patients encounter following the cancer diagnosis and inception of treatment. Specifically, the daughters' accounts demonstrate that the diagnosis intensified their bond with their ill mother, while also presenting challenges in their relationship. It precipitated a re‐definition of personal values and altered their perceived future. Their mother's illness prompted recognition of increased family risk and rendered daughters with a heightened sense of personal vulnerability. Clinicians need to appreciate the extent to which daughters can be impacted by their mother's cancer experience. Copyright © 2004 John Wiley & Sons, Ltd.
The Capsid Protein Encoded by UL17 of Herpes Simplex Virus 1 Interacts with Tegument Protein VP13/14

ABSTRACT The UL17 protein (pUL17) of herpes simplex virus 1 (HSV-1) likely associates with the surfaces of DNA-containing capsids in a heterodimer with pUL25. pUL17 is also associated with viral light particles that lack capsid proteins, suggesting its presence in the tegument of the HSV-1 virion. To help determine how pUL17 becomes incorporated into virions and its functions therein, we identified pUL17-interacting proteins by immunoprecipitation with pUL17-specific IgY at 16 h postinfection, followed by mass spectrometry. Coimmunoprecipitated proteins included cellular histone proteins H2A, H3, and H4; the intermediate filament protein vimentin; the major HSV-1 capsid protein VP5; and the HSV tegument proteins VP11/12 (pUL46) and VP13/14 (pUL47). The pUL17-VP13/14 interaction was confirmed by coimmunoprecipitation in the presence and absence of intact capsids and by affinity copurification of pUL17 and VP13/14 from lysates of cells infected with a recombinant virus encoding His-tagged pUL17. pUL17 and VP13/14-HA colocalized in the nuclear replication compartment, in the cytoplasm, and at the plasma membrane between 9 and 18 h postinfection. One possible explanation of these data is that pUL17 links the external face of the capsid to VP13/14 and associated tegument components.
Single-nucleotide polymorphisms in the FSH receptor gene and ovarian performance: Future role in IVF

The ovarian response to follicle stimulating hormone (FSH) stimulation in assisted conception cycles is variable. Although it would be beneficial to predict accurately the response of patients to FSH, to date no absolute predictors of ovarian performance have been identified. Recently, there have been a number of studies on the effect of single-nucleotide polymorphisms (SNPs) in the FSH receptor gene and its predictability in ovarian response to FSH stimulation. Several reports have shown that two very common SNPs at positions 307 and 680 in exon 10 of the FSH receptor gene are associated with ovarian response in IVF. The SNPs in exon 10 result in four discrete allelic variants characterised by the amino acid combinations Thr307-Asn680, Ala307-Ser680, Ala307-Asn680 and Thr307-Ser680. Because Thr307 is almost always in linkage disequilibrium with Asn680, and Ala307 almost always with Ser680, most studies are focussed solely on position 680. Some authors have shown predictability of ovarian response to FSH stimulation in patients with different alleles, while others have refuted this finding. In vitro models have not shown any difference in response among various alleles. Most of the available studies are retrospective, observational. Until now, there is no clear clinical benefit in the screening for SNP before IVF treatment. However, there is the prospect of devising mathematical models using a group of polymorphisms to provide an important tool for improving ovulation induction, especially in poor responders.
Fine structure and cytochemistry of the morphogenesis of round-headed human sperm.

Ejaculates and testicular biopsies of two infertile men were examined at ultrastructural and cytochemical levels. The two patients presented spermiograms in which all the spermatozoa had globular heads. Wide Golgi areas and large masses of annulatae lamellae were evident during spermatogenesis. Abnormal acrosomal vesicles were evident during early spermatid stage. Among the late spermatids, there was a small group characterized by a fibrous sheath showing considerable malformation, and "spindle shaped body. " In Sertoli cells, detached acrosomes undergoing degeneration were noted. Low TPPase activity was found in Golgi complex and in abnormal acrosomal vesicles of early spermatids. As regards acid phosphatase, was localized in spermatocytes and in early spermatids, but not in late spermatids and mature spermatozoa. Leydig cells had high phosphatase activity.
Relationship of 133Xe Cerebral Blood Flow to Middle Cerebral Arterial Flow Velocity in Men at Rest

Cerebral blood flow (CBF) was measured by 133Xe clearance simultaneously with the velocity of blood flow through the left middle cerebral artery (MCA) over a wide range of arterial PCO2 in eight normal men. Average arterial PCO2, which was varied by giving 4% and 6% CO2 in O2 and by controlled hyperventilation on O2, ranged from 25.3 to 49.9 mm Hg. Corresponding average values of global CBF15 were 27.2 and 65.0 ml 100 g min−1, respectively, whereas MCA blood-flow velocity ranged from 42.8 to 94.2 cm/s. The relationship of CBF to MCA blood-flow velocity over the imposed range of arterial PCO2 was described analytically by a parabola with the equation: C B F = 22.8 − 0.17 × v e l o c i t y + 0.006 × v e l o c i t y 2 The observed data indicate that MCA blood-flow velocity is a useful index of CBF response to change in arterial PCO2 during O2 breathing at rest. With respect to baseline values measured while breathing 100% O2 spontaneously, percent changes in velocity were significantly smaller than corresponding percent changes in CBF at increased levels of arterial PCO2 and larger than CBF changes at the lower arterial PCO2. These observed relative changes are consistent with MCA vasodilation at the site of measurement during exposure to progressive hypercapnia and also during extreme hyperventilation hypocapnia.
Overcoming Challenges: Pacific students’ experiences of being resilient through tertiary education

The issue of raising Pasifika achievement levels in New Zealand has been at the forefront of Pasifika research as early as 1996 in the AIMHI report from the Ministry of Education. Nearly, 20 years on and the goal remains the same as Pasifika still underperform in education. In the last 19 years, there has been countless research in understanding the issue, with numerous identified areas of concern. However, so many areas need addressing that is can serve the purpose of perpetuating the problem by making it too large to solve. The structural, systemic issues still remain, the lack of Pasifika teachers and teachers that understand Pasifika still remain, the gap between home and school life still exist, as well as the lack of real leaders and governance in schools that want to make a difference to the education realties of Pasifika (Chu et al., 2013). This frustration has led to the rise of “strength based” approached to Pasifika education research in pursuit of solutions. With a focus on appreciating what works for Pasifika and trying to foster that success and replicate it. However, a gap remains, there has been little to no platform for the empowered Pasifika voice on the issue. The Pasifika person that “bucks” the trend and despite the countless education issues were resilient and fought for their educational success. This thesis provides a platform for this voice. This thesis reveals the stories of eight Pasifika postgraduate students (PPGS) that were deemed academic failures at secondary school. It focuses on their internal and external factors that facilitated their academic progress and resilience. This qualitative study was underpinned by appreciative inquiry as the theoretical framework and a Pacific methodology and method Talanoa. The participants were selected via snowball sampling technique and one on one Talanoa discussions were used to explore their stories. The main finding from the research identified that as the participant’s self-concepts changed so did their academic performance. The identified historical and recent education barriers were still present in both their education failure and success. However, through transferable success experiences, deep reflection, visualisation, and goal setting, enabled the participants to see a positive academic result before it eventuated. This helped the participants develop positive self-concepts and attitudes that facilitated their progress and resilience to overcome existing barriers and become successful postgraduate students.
ICARUS: Identification of complementary algorithms by uncovered sets

Since there is no single best performing algorithm for all problems, an algorithm portfolio would leverage the strengths of complementary algorithms to achieve the best performance. In this paper, we present and evaluate a new technique for designing algorithm portfolios for continuous black-box optimization problems, based on social choice and voting theory concepts. Our technique, which we call ICARUS, models the portfolio design task as an election, in which each problem `votes' for a subset of preferred algorithms guided by a performance metric such as the number of fitness evaluations. The resulting `uncovered set' of algorithms forms the portfolio. We demonstrate the efficacy of ICARUS using a suite of state-of-the-art evolutionary algorithms and benchmark continuous optimization problems. Our analysis confirms that ICARUS creates an algorithm portfolio where the expected performance is superior to a manually constructed portfolio.
Polarized Distribution of Endogenous Rac1 and RhoA at the Cell Surface*

Rac1 and RhoA regulate membrane ruffling and stress fiber formation. Both molecules appear to exert their control from the plasma membrane. In fibroblasts stimulated with platelet-derived growth factor or lysophosphatidic acid, the reorganization of the cytoskeleton begins at specific sites on the cell surface. We now report that endogenous Rac1 and RhoA also have a polarized distribution at the cell surface. Cell fractionation and immunogold labeling show that in quiescent fibroblasts both of these molecules are concentrated in caveolae, which are plasma membrane domains that are associated with actin-rich regions of the cell. Treatment of these cells with platelet-derived growth factor stimulated the recruitment of additional Rac1 and RhoA to caveolae fractions, while lysophosphatidic acid only caused the recruitment of RhoA. We could reconstitute the recruitment of RhoA using either whole cell lysates or purified caveolae. Surprisingly, pretreatment of the lysates with exoenzyme C3 shifted both resident and recruited RhoA from caveolae to noncaveolae membranes. The shift in location was not caused by inactivation of the RhoA effector domain. Moreover, chimeric proteins containing the C-terminal consensus site for Rac1 and RhoA prenylation were constitutively targeted to caveolae fractions. These results suggest that the polarized distribution of Rho family proteins at the cell surface involves an initial targeting of the protein to caveolae and a mechanism for retaining it at this site.
Neurodegenerative differences in the retinas of male and female patients with type 2 diabetes.

PURPOSE The purpose of our study is to determine whether neuroretinal function, measured by the multifocal electroretinogram, differs between males and females with type 2 diabetes and no retinopathy. METHODS This study included 70 eyes from 70 adult subjects (14 control males, 22 control females, 16 males with type 2 diabetes, and 18 females with type 2 diabetes). A template-scaling technique was used to obtain first-order P1 implicit times and N1-P1 amplitudes from photopic multifocal electroretinograms within the central 45 degrees. RESULTS The males with type 2 diabetes were significantly more abnormal than their female counterparts in two separate analyses of local neuroretinal function. First, the total number of retinal locations with an abnormally delayed implicit time (z score ≥ 2) was higher (P < 0.001) in the diabetic males (482 locations = 29.2%) compared to the diabetic females (298 locations = 16.1%). Second, in the response topographies that consisted of 103 means of local implicit times for each group, the diabetic males were significantly delayed (P < 0.025) at 23 corresponding positions (22.3%) compared to the diabetic females. At the same time, no corresponding stimulus locations were significantly delayed in the diabetic females compared to the diabetic males. CONCLUSIONS Neuroretinal function is more abnormal in males than in females for adults with type 2 diabetes and no retinopathy. These results suggest that, relative to males, females may have some protection from, or resistance to, neurodegenerative changes that precede the development of background retinopathy in type 2 diabetes.
First Evidence of an Established Population of Ixodes scapularis (Acari: Ixodidae) in South Dakota

Abstract Ixodes scapularis Say (Acari: Ixodidae) is the most important vector of human disease in the United States. Recent efforts by public health officials to determine its presence on a county-by-county basis have been undertaken to assist in Lyme disease risk assessment. Recent modeling efforts show that South Dakota can potentially support populations of I. scapularis based on favorable climatic conditions and presence of suitable hosts to support tick populations within the state. We provide the first documentation of an established population of I. scapularis in Clay County, SD, providing only the third record of the presence of this tick species within the state.
Are junction potentials essential? Dual mechanism of smooth muscle cell activation by transmitter released from autonomic nerves.

Stimulation of autonomic nerves supplying smooth muscle tissues often evokes either an e.j.p. or an i.j.p. which involves a shift in the membrane potential such that the entry of calcium into the cell through voltage-sensitive calcium channels is likely to be increased or decreased respectively. In smooth muscle which freely discharges action potentials either spontaneously or in response to excitatory influences in vivo, the change in membrane potential will alter the rate of action potential discharge and so the tension developed by the smooth muscle. In this way there is modulation of the rate of entry of calcium, or its release within the cell, by a voltage-dependent mechanism entrained by the junction potential, as the action potential represents the operation of voltage-dependent calcium channels. In smooth muscles not freely discharging action potentials, the numbers of open calcium channels may change with depolarization, or even hyperpolarization, so the junction potential again brings into play a voltage-dependent mechanism of calcium entry. However, a dual mechanism of neurotransmission seems to operate at many autonomic nerve-smooth muscle junctions such that voltage-independent mechanisms coexist with voltage-dependent mechanisms. It is now generally accepted that activation of receptors on smooth muscle cells caused by bathing smooth muscles in solutions containing excitatory transmitters can bring into operation processes which are not voltage-dependent and which do not depend on modulation of the rate of action potential discharge. Up to the present it has been by no means certain that when released from autonomic nerves these excitatory transmitters (ACh, noradrenaline, substance P and n.a.n.c. excitatory transmitters) could also act in the same voltage-independent way - not least because studies of smooth muscle cells at the cellular level have been dominated by membrane potential recording (by micro-electrode) which has revealed the e.j.p. or some form of depolarization as a seemingly ubiquitous feature of excitatory autonomic junctions of nerve and smooth muscle cells. However, experiments show that under conditions when the e.j.p. and/or the action potential are abolished or severely impaired by drug application, substantial nerve-evoked smooth muscle contractions may occur at many autonomic junctions. Conversely, severe impairment of nerve-evoked contraction may occur in some cases with excitatory-receptor antagonists without loss, or even in some cases any impairment, of the e.j.p. which presumably depends on a different receptor type. (ABSTRACT TRUNCATED AT 400 WORDS)
Relationship between in vitro chemosensitivity assessed with MTT assay and clinical outcomes in 103 patients with acute leukemia.

BACKGROUND Cellular drug resistance is supposed to play a major role in chemotherapy failure or relapse. The purpose of this study was to analyze the relationship between in vitro chemosensitivity test results using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and clinical response on chemotherapy, and to find the possibility of optimizing the treatment protocol for individual patients according to their actual drug resistance. METHODS For MTT assay, we obtained bone marrow aspirates from 103 patients with acute leukemia at the time of initial diagnosis or relapse. The following drugs were tested: cytarabine, vincristine, methotrexate, daunorubicin, dexamethasone, L-asparaginase, and mitoxantrone. To evaluate clinical responses after induction chemotherapy, we followed up on their bone marrow study. RESULTS In our study, in vitro chemosensitivity test with the MTT assay significantly predicted whether patients with AML remained continuous complete remission or went into relapse. It also predicted whether or not child patients with ALL would acquire complete remission after induction chemotherapy. CONCLUSIONS Although it does not provide the insight into the mechanisms that cause drug resistance, the MTT assay may be a useful tool in individually optimizing the chemotherapy of patients with acute leukemia.
Group A streptococcal phagocytosis resistance is independent of complement factor H and factor H‐like protein 1 binding

Factor H (FH) and factor H‐like protein 1 (FHL‐1) regulate complement activation through the alternative pathway. Several extracellular bacterial pathogens, prime targets for the complement system, bind FH and FHL‐1, thereby acquiring a potential mechanism for minimizing complement deposition on their surface. For group A streptococci (GAS), surface‐bound antiphagocytic M proteins mediate the interaction. To study the role of the FH–FHL‐1 interaction for complement deposition and opsonophagocytosis of GAS, we first constructed a set of truncated M5 protein variants and expressed them on the surface of a homologous M‐negative GAS strain. Binding experiments with the resulting strains demonstrated that the major FH–FHL‐1 binding is located in a 42‐amino‐acid region within the N‐terminal third of M5. Measurement of bacteria‐bound complement factor C3 after incubation in plasma showed that the presence of this region had little impact upon complement deposition through the alternative pathway. Moreover, streptococci expressing M5 proteins lacking the major FH and FHL‐1 binding sequence resisted phagocytosis in human blood as efficiently as bacteria expressing the wild‐type protein. Consequently, the data suggest that the binding of the regulators of the alternative pathway is of limited importance for GAS phagocytosis resistance.
A day in the life of third-year medical students: using an ethnographic method to understand information seeking and use*

Objective The authors undertook this project to learn how third-year medical students seek and use information in the course of daily activities, especially activities conducted in clinical settings in a variety of institutions. Methods We recruited sixty-eight third-year undergraduate medical school students to create a mapping diary of a day that included clinical activities. We conducted semi-structured interviews based on the mapping diaries. Using content and thematic analyses of the resulting interview transcripts, we developed an ethnographic case study for each participant. Results In the studied sample, we identified a broad range of information resources used for personal, clinical, and educational use. Participants relied heavily on technology throughout their day, including desktop computers, smart phones, handheld tablets, and laptops. Time management was a pervasive theme in the interviews, with participants squeezing in time to study for exams wherever and whenever they could. Selection of a particular information resource or technology to use was governed largely by the convenience of using that resource or technology. When obstacles were encountered, workarounds might be sought, but in many cases, the resource or technology would be abandoned in favor of a more convenient solution. Convenience was also a consideration in choosing spaces to use for clinical duties or for study, with specific considerations of available technology, proximity to clinical areas, and security for belongings contributing to choices made. Conclusions Some of our results align with those of other recent studies of information use among medical students, residents, and practicing physicians. In particular, the fast-paced clinical setting favors use of information resources that are fast and easy to use. We demonstrated that the methods used are suitable to better understand clinicians’ discovery and use of information.
The Dynamic Conduct of Bone Marrow-Derived Cells in the Choroidal Neovascularization Microenvironment

Purpose: Choroidal neovascularization (CNV) is one of the most frequent causes of severe and progressive vision loss. Prior studies have shown that bone marrow-derived cells (BMCs) play an important role in CNV, indicating that BMCs can be a potential target for inhibiting the development of CNV. It could be helpful for our understanding of CNV to study the dynamic conduct of BMCs in the CNV microenvironment. Methods: Green fluorescent protein (gfp) chimeric mice were developed by transplanting bone marrow cells from gfp+/+ transgenic mice to adult C57BL/6J mice. The chimeric mice underwent laser rupture of Bruch's membrane to induce CNV and were killed at 1, 2, 3, and 4 weeks after laser injury. The eyes were enucleated and processed for immunofluorescence to detect markers for vascular smooth muscle cells (α smooth muscle actin, α SMA), endothelial cells (CD31), or macrophages (F4/80) on gfp+ cells. All sections were qualitatively and quantitatively assessed by confocal microscopy. Results: Large number of gfp-labeled cells appeared in the lesions and integrated into CNV. Gfp+ cells, which were immunoreactive for α SMA, CD31, or F4/80, can be detected through the whole study. The constituent ratio of those three cell-types in total gfp+ cells in CNV altered as CNV developed. The maximal ratios of CD31-labeled cells and F4/80-labeled cells presented at 2 week, while the ratio of α SMA-labeled cells upgraded continuously. Conclusions: BMCs underwent a serial of changes in position and expression during the progression of CNV. Those changes may result from the interaction between BMCs and the CNV microenvironment.