Miracle-dz/UniFAR-FTUs huggingface dataset:
Body painting to promote self-active learning of hand anatomy for preclinical medical students

Background The purpose of this study was to use the body painting method to teach hand anatomy to a group of preclinical medical students. Methods Students reviewed hand anatomy using the traditional method and body painting exercise. Feedback and retention of the anatomy-related information were examined by a questionnaire and multiple-choice questions, respectively, immediately and 1 month after the painting exercise. Results Students agreed that the exercise was advantageous and helped facilitate self-active learning after in-class anatomy lessons. While there was no significant difference in knowledge retention between the control and experimental groups, the students appreciated the exercise in which they applied body paint to the human body to learn anatomy. Conclusion The body painting was an efficient tool for aiding the interactive learning of medical students and increasing the understanding of gross anatomy.
Out of the Orient: Post‐Tethyan transoceanic and trans‐Arabian routes fostered the spread of Baorini skippers in the Afrotropics

The origin of taxa presenting a disjunct distribution between Africa and Asia has puzzled biogeographers for more than a century. This biogeographic pattern has been hypothesized to be the result of transoceanic long‐distance dispersal, Oligocene dispersal through forested corridors, Miocene dispersal through the Arabian Peninsula or passive dispersal on the rifting Indian plate. However, it has often been difficult to pinpoint the mechanisms at play. We investigate biotic exchange between the Afrotropics and the Oriental region during the Cenozoic, a period in which geological changes altered landmass connectivity. We use Baorini skippers (Lepidoptera, Hesperiidae) as a model, a widespread clade of butterflies in the Old World tropics with a disjunct distribution between the Afrotropics and the Oriental region. We use anchored phylogenomics to infer a robust evolutionary tree for Baorini skippers and estimate divergence times and ancestral ranges to test biogeographic hypotheses. Our phylogenomic tree recovers strongly supported relationships for Baorini skippers and clarifies the systematics of the tribe. Dating analyses suggest that these butterflies originated in the Oriental region, Greater Sunda Islands, and the Philippines in the early Miocene c. 23 Ma. Baorini skippers dispersed from the Oriental region towards Africa at least five times in the past 20 Ma. These butterflies colonized the Afrotropics primarily through trans‐Arabian geodispersal after the closure of the Tethyan seaway in the mid‐Miocene. Range expansion from the Oriental region towards the African continent probably occurred via the Gomphotherium land bridge through the Arabian Peninsula. Alternative scenarios invoking long‐distance dispersal and vicariance are not supported. The Miocene climate change and biome shift from forested areas to grasslands possibly facilitated geodispersal in this clade of butterflies.
A Study of Suicide Rates in Northern Ireland 1984–2002

Annual figures collected by the Samaritans from the Registrar Generals' figures for suicides for the years 1984–2002 inclusive were analysed. Trends by gender, age group, marital status and method were examined. Suicide rates were standardised where appropriate. The mean annual rate was calculated for the 10 year period 1984–1993 and compared with the nine year period 1994–2002. The mean annual rate of suicide increased by 4.7%. Female suicide rates decreased by 17%, male suicides increased by 13.2%. The highest percentage increase was seen in males aged 25–34, (34%) followed by the 15–24 age group, (26.5%). There was a significant upward trend in suicide rates at p<0.01 in young males aged 10–34 and a significant fall in total suicide rates in those aged 35+. The greatest increase in the mean annual rate was seen in those of single status in sexes, males 24.2% and females 28.6%. There was a decrease in the mean annual rate for all methods of suicide except hanging with an increase of 99.37% in males and 87.80% in females. The overall rate of suicide in Northern Ireland appears to be rising. This trend is largely a result of the increase in suicides amongst young males aged 10–34. Violent methods of suicide, namely hanging have increased, suggesting that this more lethal method is contributing to the higher suicide rate.
Revisiting retesting in the estimation of proportions by group testing

ABSTRACT In the estimation of a proportion p by group testing (pooled testing), retesting of units within positive groups has received little attention due to the minimal gain in precision compared to testing additional units. If acquisition of additional units is impractical or too expensive, and testing is not destructive, we show that retesting can be a useful option. We propose the retesting of a random grouping of units from positive groups, and compare it with nested halving procedures suggested by others. We develop an estimator of p for our proposed method, and examine its variance properties. Using simulation we compare retesting methods across a range of group testing situations, and show that for most realistic scenarios, our method is more efficient.
Drivers of C cycling in three arctic-alpine plant communities

ABSTRACT Recent vegetation changes in arctic-alpine tundra ecosystems may affect several ecosystem processes that regulate microbe and soil functions. Such changes can alter ecosystem carbon (C) cycling with positive feedback to the atmosphere if plant C uptake is less than the amount of soil C released. Here, we examine how differences in plant functional traits, microbial activity, and soil processes within and across Salix-dominated shrub, dwarf shrub–dominated heath, and herb- and cryptogam-dominated meadow communities influence C cycling. We develop a hypothesized framework based on a priori model selection of variation in daytime growing season gross ecosystem photosynthesis (GEP) and above- and belowground respiration. The fluxes were standardized to light and temperature. Gross ecosystem photosynthesis was primarily related to soil moisture and secondarily to plant functional traits and aboveground biomass, and belowground respiration was dependent on the community weighted mean of specific leaf area (SLACWM). Similarly, microbial activity was linked with SLACWM and was highest in meadows, and carbon-degrading microbial activity decreased with vegetation woodiness. These results suggest that shrub expansion may influence summer C cycling differently depending on plant community, as belowground respiration might increase in the heath and decrease in the meadow communities.
Effect of Vasodilator Drugs on Coronary Occlusion and Reperfusion Arrhythmias in Anesthetized Dogs

Summary: The effect of vasodilator drugs on the incidence of ventricular arrhythmias induced during 30 min of occlusion and 15 min of reperfusion of the left anterior descending coronary artery (LAD) was studied in 65 pentobarbital-anesthetized open-chest dogs. Intravenous administration of captopril (0.5 mg/kg), enalapril (0.5 mg/kg), felodipine (4 μg/kg), or ketanserin (0.1 mg/kg) 30 min before LAD occlusion reduced mean arterial blood pressure by 15.5 ± 0.6% (mean ± SEM). Nifedipine (5 μg/kg bolus + 1 μg/kg min−1) infusion reduced mean arterial blood pressure by 24.8 ± 1.8%. In none of the dogs was the diastolic blood pressure reduced below 70 mm Hg. During LAD occlusion, reduction in arterial blood pressure by these drugs was associated with a reduced incidence of ventricular premature depolarizations, ventricular tachycardia, and ventricular fibrillation (VF). During LAD reperfusion, the incidence of VF in saline-treated animals was 6/9, whereas for captopril it was 6/9, enalapril 1/9, felodipine 7/9, nifedipine 3/8, and ketanserin 3/9 animals. Thus, only enalapril significantly lowered the incidence of VF (p < 0.05). The mechanism responsible for this antifibrillatory effect of enalapril is unknown. The muscle mass of the left ventricle supplied by the LAD distal to the site of occlusion in dogs which survived was similar to that of dogs which developed ventricular fibrillation.
Ossifying spinal arachnoiditis

Chronic spinal arachnoiditis is a well recognised condition in which foci of calcification are known to occur, but in which true ossification is extremely rare. There have probably been only 17 examples of this condition described previously. Most cases of arachnoiditis ossificans have been discovered at laminectomy and the rest at post-mortem examination. Many of the cases presented without there being any clue as to causation, but spinal subarachnoid haemorrhage and spinal anaesthesia have appeared to be implicated in others. We are reporting a case of arachnoiditis with ossification which affected the cauda equina, and which was discovered 8 years after previous investigation and operation for lumbo-sacral disc protrusion. Case History (M. C. N. N. 68/293)
Spermatogenesis in mice is not affected by histone H1.1 deficiency.

The linker histone subtype H1.1 belongs to the group of main-type histones and is synthesized in somatic tissues as well as in germ cells during the S phase of the cell cycle. In adult mice the histone gene H1.1 is expressed mainly in thymus, spleen, and testis. The single-copy gene coding for the H1.1 protein was eliminated by homologous recombination in mouse embryonic stem cells. Mice homozygous for the deficient H1.1 gene developed normally until the adult stage without H1.1 mRNA and H1.1 protein. No anatomic abnormalities could be detected. In addition, mice lacking the H1.1 gene were fertile and they showed normal spermatogenesis and testicular morphology.
Same Systems, Different Outcomes

Summary Objectives: To compare the outcome of the implementation of computerized physician order entry (CPOE) systems in two Dutch hospitals. Methods: Qualitative research methods, including interviews in both hospitals, observations of system in use, observations of staff meetings and document analysis were used to understand the implementation of CPOE. The transcribed texts and implementation documents were analyzed for relevant concepts. The transcripts and field notes were analyzed using a heuristic success and failure model with medical work as the primary focus. Results: Occasions that determined the outcome of the implementation were classified according to factors that may influence the success or failure of implementing systems. Conclusions: The themes and patterns that emerged from the data helped validate the concept of medical work as the primary focus of our analysis model; in addition the concept of a support base necessary to accept changes in medical work that result from introducing CPOE may help to understand the different implementation outcomes.
A microgrid with PV production and energy storage for an university building

The paper describes a measurement campaign conducted at the Engineering Department of Astronautics, Electrical and Energetic, Sapienza University of Rome. Starting from measurement campaign, in the context of building an urban microgrid, a PV plant and a energy storage system has been proposed. The measurements were conducted in two relevant period of the year in order to know the power consumption of the facility. The knowledge of total energy demand by electrical loads allows the identification of most efficient strategies for energy utilization by using the PV production and energy storage.
A comparison of parthenogenetic and sexual embryogenesis of the pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea).

Aphids exhibit divergent modes of embryogenesis during the sexual and asexual phases of the life cycle. To explore how a single genome can give rise to these alternative developmental modes, we have initiated embryological studies of the pea aphid, Acyrthosiphon pisum. Here we present a detailed description of parthenogenetic, viviparous embryonic development in the pea aphid. We compare and contrast development of the parthenogenetic embryo with that of the embryo resulting from sexual reproduction. The primary difference between the embryos is the scale on which development occurs: early parthenogenetic development occurs in a volume approximately three orders of magnitude smaller than the sexual egg, largely because of the apparent absence of yolk in the parthenogenetic egg. This results in a drastically different duration of syncytial energid cleavage and, presumably, patterning processes in the two embryos must act at scales that differ by orders of magnitude. The eggs also develop on time scales that differ approximately by an order of magnitude and the timing of the embryonic movements, collectively called blastokinesis, have temporally shifted relative to growth of the embryo. In addition, the endosymbiotic bacteria are transferred from mother to embryo in different ways in the two embryos. Finally, the function of the serosa has diverged greatly in the two embryos: in the sexual egg the serosa deposits a thick cuticle that protects the egg, whereas the serosa of the parthenogenetic embryo is greatly reduced and its function is unclear. The pea aphid is a useful model system for examining how a single genome has evolved to allow divergent modes of development.
A novel activating mutation in the thyrotropin receptor gene in an autonomously functioning thyroid nodule developed by a Japanese patient.

OBJECTIVE A number of activating mutations of the thyrotropin receptor (TSHR) have been found in autonomously functioning thyroid nodules (AFTNs) in European patients. We aimed to study TSHR mutation in AFTNs in Japanese patients because no TSHR activating mutation has been found by previous incomplete studies. DESIGN A typical AFTN developed in a 69-year-old Japanese woman was studied. METHODS The entire exon 10 of the TSHR cDNA was sequenced. Functional studies were done by site-directed mutagenesis and transfection of a mutant construct into COS-7 cells. RESULTS We identified a novel heterozygous TSHR gene mutation, Leu512-->Arg (L512R; CTG-->CCG), from the AFTN. The mutation was not detected in the adjacent normal thyroid tissue. COS-7 cells transfected with L512R mutant TSHR expression vector exhibited a 3.3-fold increase in basal cAMP level compared with that of cells transfected with wild-type TSHR DNA, confirming that the mutation was the direct cause of the AFTN. TSHR activating mutations involving the third transmembrane helix reported to date are S505R/N and V509A as well as L512R. An in vitro site-directed mutagenesis study encompassing residues 505-513 revealed that mutations involving residues other than these three did not show constitutive activation. CONCLUSION This is the first TSHR activating mutation found in a Japanese patient, although true prevalence of TSHR activating mutations in AFTNs developed in Japanese patients remains to be elucidated. In addition, functional studies suggested that amino acid residues in the third transmembrane helix maintaining inactive conformation of the TSHR seem to be located on the same surface of the alpha-helix, possibly making interhelical bonds with another helix.
Psychometric Evaluation of the Chinese Respiratory Distress Observation Scale on Critically Ill Patients With Cardiopulmonary Diseases

Background: Respiratory distress is a common and distressing experience that strikes most critically ill patients with cardiopulmonary diseases. Now, most dyspnea assessment tools rely on patient’s self-report. Only one instrument, the Respiratory Distress Observation Scale (RDOS), is valid for assessing respiratory distress when the patient cannot provide a dyspnea self-report. Purpose: The aims of this study were to develop the Chinese version of RDOS through two-way translation and examine its reliability and validity. Methods: The first step was the translation and back-translation to establish the Chinese RDOS and examine the semantic equivalence between the back-translation version and the original one. The second step was the psychometric testing of the Chinese RDOS including internal reliability, interrater reliability, concurrent validity, convergent validity, and content and construct validity. Results: The Chinese RDOS was reported with an internal consistency (Cronbach’s &agr; value) of . 73, an interobserver reliability of . 97, and a content validity index of . 97. In the conscious critically ill patients, significant correlations were found between the Chinese RDOS and Spielberger State Anxiety Inventory scores or Dyspnea Vertical Visual Analog Scale scores (r = .69 and r = .76, respectively; p < .001). In the unconscious critically ill patients, there was a significantly positive correlation between the Chinese RDOS scores and fraction of inspiration O2 (r = .26, p < .05) and a significantly negative correlation between the Chinese RDOS scores and saturation peripheral oxygen (SpO2; r = −.42, p = .001). In addition, it also could effectively identify the dyspnea differences between the first and last days in the intensive care units (p < .001). Conclusions: The Chinese RDOS showed good reliability and validity in conscious critically ill patients. The assessment with the Chinese RDOS could be completed within a short period no matter whether the assessor has nursing background in respiratory care or not. It could help the professionals systematically manage and track the change of respiratory distress in patients.
Melittin prevents liver cancer cell metastasis through inhibition of the Rac1‐dependent pathway

Melittin, a water‐soluble toxic peptide derived from bee venom of Apis mellifera was reported to have inhibitory effects on hepatocellular carcinoma (HCC). However, its role in antimetastasis and the underlying mechanism remains elusive. By utilizing both HCC cell lines and an animal model based assay system, we found that Rac1, which has been shown to be involved in cancer cell metastasis, is highly expressed in aggressive HCC cell lines and its activity correlated with cell motility and cytoskeleton polymerization. In addition, Rac1‐dependent activity and metastatic potential of aggressive HCC cells are remarkably high in both cellular and nude mouse models. We provide evidence here that melittin inhibits the viability and motility of HCC cells in vitro, which correlates with its suppression of Rac1‐dependent activity, cell motility, and microfilament depolymerization. Furthermore, melittin suppresses both HCC metastasis and Rac1‐dependent activity in nude mouse models. The specificity of the effect of melittin on Rac1 was confirmed in HCC cells both in vitro and in vivo. Conclusion: Melittin inhibits tumor cell metastasis by reducing cell motility and migration via the suppression of Rac1‐dependent pathway, suggesting that melittin is a potential therapeutic agent for HCC. (HEPATOLOGY 2008;47:1964–1973.)
Fine-Scale Community Structure Analysis of ANME in Nyegga Sediments with High and Low Methane Flux

To obtain knowledge on how regional variations in methane seepage rates influence the stratification, abundance, and diversity of anaerobic methanotrophs (ANME), we analyzed the vertical microbial stratification in a gravity core from a methane micro-seeping area at Nyegga by using 454-pyrosequencing of 16S rRNA gene tagged amplicons and quantitative PCR. These data were compared with previously obtained data from the more active G11 pockmark, characterized by higher methane flux. A down core stratification and high relative abundance of ANME were observed in both cores, with transition from an ANME-2a/b dominated community in low-sulfide and low methane horizons to ANME-1 dominance in horizons near the sulfate-methane transition zone. The stratification was over a wider spatial region and at greater depth in the core with lower methane flux, and the total 16S rRNA copy numbers were two orders of magnitude lower than in the sediments at G11 pockmark. A fine-scale view into the ANME communities at each location was achieved through operational taxonomical units (OTU) clustering of ANME-affiliated sequences. The majority of ANME-1 sequences from both sampling sites clustered within one OTU, while ANME-2a/b sequences were represented in unique OTUs. We suggest that free-living ANME-1 is the most abundant taxon in Nyegga cold seeps, and also the main consumer of methane. The observation of specific ANME-2a/b OTUs at each location could reflect that organisms within this clade are adapted to different geochemical settings, perhaps due to differences in methane affinity. Given that the ANME-2a/b population could be sustained in less active seepage areas, this subgroup could be potential seed populations in newly developed methane-enriched environments.
The Arabidopsis COBRA Protein Facilitates Cellulose Crystallization at the Plasma Membrane*

Background: The COBRA gene is highly coexpressed with cellulose synthase genes, but its function remains unclear. Results: COBRA localizes at the plasma membrane and binds glucan chains. NMR studies indicate structural defects in cellulose in the mutant despite normal polymerization rate. Conclusion: COBRA functions downstream of cellulose biosynthesis. Significance: This work suggests that alignment of glucan chains into cellulose fibrils is facilitated by one or more proteins. Mutations in the Arabidopsis COBRA gene lead to defects in cellulose synthesis but the function of COBRA is unknown. Here we present evidence that COBRA localizes to discrete particles in the plasma membrane and is sensitive to inhibitors of cellulose synthesis, suggesting that COBRA and the cellulose synthase complex reside in close proximity on the plasma membrane. Live-cell imaging of cellulose synthesis indicated that, once initiated, cellulose synthesis appeared to proceed normally in the cobra mutant. Using isothermal calorimetry, COBRA was found to bind individual β1–4-linked glucan chains with a KD of 3.2 μm. Competition assays suggests that COBRA binds individual β1–4-linked glucan chains with higher affinity than crystalline cellulose. Solid-state nuclear magnetic resonance studies of the cell wall of the cobra mutant also indicated that, in addition to decreases in cellulose amount, the properties of the cellulose fibrils and other cell wall polymers differed from wild type by being less crystalline and having an increased number of reducing ends. We interpret the available evidence as suggesting that COBRA facilitates cellulose crystallization from the emerging β1–4-glucan chains by acting as a “polysaccharide chaperone. ”