Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification and Functional Characterization of Lsm Proteins in Trypanosoma brucei*

RNA interference of Sm proteins in Trypanosoma brucei demonstrated that the stability of the small nuclear RNAs (U1, U2, U4, U5) and the spliced leader RNA, but not U6 RNA, were affected upon Sm depletion (Mandelboim, M., Barth, S., Biton, M., Liang, X. H., and Michaeli, S. (2003) J. Biol. Chem. 278, 51469–51478), suggesting that Lsm proteins that bind and stabilize U6 RNA in other eukaryotes should exist in trypanosomes. In this study, we identified seven Lsm proteins (Lsm2p to Lsm8p) and examined the function of Lsm3p and Lsm8p by RNA interference silencing. Both Lsm proteins were found to be essential for U6 stability and mRNA decay. Silencing was lethal, and cis- and trans-splicing were inhibited. Importantly, silencing also affected the level of U4.U6 and the U4.U6/U5 tri-small nuclear ribonucleoprotein complexes. The presence of Lsm proteins in trypanosomes that diverged early in the eukaryotic lineage suggests that these proteins are highly conserved in both structure and function among eukaryotes. Interestingly, however, Lsm1p that is specific to the mRNA decay complex was not identified in the genome data base of any kinetoplastidae, and the Lsm8p that in other eukaryotes exclusively functions in U6 stability was found to function in trypanosomes also in mRNA decay. These data therefore suggest that in trypanosomes only a single Lsm complex may exist.
Ultrathin Direct Atomic Layer Deposition on Composite Electrodes for Highly Durable and Safe Li‐Ion Batteries

Abstract : In order to employ Li-ion batteries (LIBs) in next-generation hybrid electric and/or plug-in hybrid electric vehicles (HEVs and PHEVs), LIBs must satisfy many requirements: electrodes with long lifetimes (fabricated from inexpensive environmentally benign materials), stability over a wide temperature range, high energy density, and high rate capability. Establishing long-term durability while operating at realistic temperatures (5000 charge-depleting cycles, 15 year calendar life, and a range from -46 deg C to +66 deg C) for a battery that does not fail catastrophically remains a significant challenge. Recently, surface modifications of electrode materials have been explored as viable paths to improve the performance of LIBs for vehicular applications. Here we clearly demonstrate that conformal ultrathin protective coating by inactive metal oxide without disrupting inter-particle electronic pathway can be realized by atomic layer deposition (ALD) directly performed on a composite electrode, which leads to significant improvement of both long-term durability and safety of NG anode. Also ALD coatings are significantly more promising than efforts that have been previously reported.
Effects of the Use of Social Network Sites on Task Performance: Toward a Sustainable Performance in a Distracting Work Environment

As the use of social network sites (SNS) has become increasingly prevalent, its effect on sustainable performance has received much attention. The existing literature has taken either a positive or negative view of SNS, arguing that it either decreases performance by taking time and effort away from work, or increases performance by providing social benefits for enhancing performance. In contrast, this experimental study, investigates how SNS use can disturb or enhance the performance of different types of tasks differently, thus influencing the sustainability of task performance. Based on distraction-conflict theory, this study distinguishes between simple and complex tasks, examines the role of SNS, and analyzes data including electroencephalography data captured by a brain-computer interface. The results show that task performance can be sustainable such that SNS use positively influences performance when participants are engaged in a simple task and influences performance neither positively nor negatively when participants are engaged in a complex task. The study finds the former result is attributable to the positive effect of the psychological arousal induced by SNS use and the latter result to the negative effect of the psychological arousal offsetting the positive effect of reduced stress resulting from SNS use.
The design of a multi-agent transformer condition monitoring system

Online diagnostics and online condition monitoring are important functions within the operation and maintenance of power transformers. This paper describes how a multi-agent system (MAS) for transformer condition monitoring has been designed to employ the data generated by the ultra high frequency (UHF) monitoring of partial discharge activity. It describes the rationale behind the use of multi-agent techniques, and the problems overcome through this technology. Every aspect of the MAS design is discussed. In addition, the design and performance of the intelligent interpretation techniques are detailed.
Do Placental Histopathologic Characteristics Differ with Gestational Ages in Preterm and Term Deliveries?

The study aim is to evaluate the placental histopathological characteristics and maternal risk factors in preterm and term births according to their weeks of gestation. We designed a prospective study involving a patient population (n = 355) composed of pregnant women who delivered preterm (n = 216) and term neonates (n = 139). The preterm births were divided into three groups as extremely (n = 22), moderate (n = 96) and late preterm (n = 98) births. The statistical analyses were performed using SPSS version 15 software. There was significant difference regarding maternal vascular underperfusion and inflamation in the extremely preterm group compared with the other groups (P = 0.001), but fetal vascular obstruction and villitis of unknown etiology were not found significantly different. According to our study results, the careful examination of the placenta of premature babies, particularly those of extremely preterm births, should be part of routine obstetrical management to determine the causes of preterm birth.
Treatment options for trigeminal neuralgia

The evidence is poor for most non-drug options, but such treatments are needed Patients describe the sudden and severe pain of trigeminal neuralgia as a “red hot needle” or “forked lightning” pain in the face. The French term “tic doloreux” emphasises the suddenness of the pain that may be triggered by touch or cold. This characteristic pain affects four to five people in 100 000. It occurs in bouts lasting weeks or months, with periods of remission of months or years. Evidence is increasing that in most patients trigeminal neuralgia is caused by compression of the trigeminal nerve root, close to its entry into the pons, by an aberrant arterial or venous loop.1 Other compressive lesions are responsible in a few patients. About 2% of patients with trigeminal neuralgia have multiple sclerosis. Standard first line treatment is carbamazepine.2 3 Other drugs including lamotrigine, phenytoin, gabapentin, oxcarbazepine, topiramate, baclofen, and clonazepam have some effect, although studies are more limited.3 Many patients fail to have …
Genetic diversity and toxic activity of Aggregatibacter actinomycetemcomitans isolates.

INTRODUCTION Very little is known of the diversity and expression of virulence factors of serotypes of Aggregatibacter actinomycetemcomitans. Toxic activity on Chinese hamster ovary (CHO) cells and cdt and ltx genotyping were evaluated in A. actinomycetemcomitans serotypes. METHODS Forty-one A. actinomycetemcomitans isolates were analysed for CHO cell growth inhibition. Genotyping was performed by polymerase chain reactions specific to the ltx promoter region, serotype-specific and cdt region and by sequencing of cdtB. RESULTS cdtABC was detected in 40 strains. Analysis of the cdtA upstream region revealed 10 cdt genotypes. Toxicity to CHO cells was detected for 92.7% of the isolates; however, no correlation between the toxic activity and the cdt genotype was detected. Serotype c was more prevalent among Brazilian samples (68.0%). Four serotype b isolates from subjects with aggressive periodontitis were associated with high leukotoxin production and exhibited moderate to strong toxic activity in CHO cells, but were classified in different cdt genotypes. High levels of toxicity in CHO cells were not associated with a particular serotype; 57.1% of serotype a isolates presented low toxicity to CHO cells whereas the highly toxic strains belonged to serotypes b and c. Sequencing of cdtB revealed a single nucleotide polymorphism of amino acid 281 but this was not related to the toxic activity in CHO cells. CONCLUSION Differences in prevalence of the low and highly cytotoxic strains among serotypes reinforce the hypothesis that serotype b and c isolates of A. actinomycetemcomitans are more virulent than serotype a strains.
Prophylaxis with single-dose doxycycline for the prevention of Lyme disease after an Ixodes scapularis tick bite.

BACKGROUND It is unclear whether antimicrobial treatment after an Ixodes scapularis tick bite will prevent Lyme disease. METHODS In an area of New York where Lyme disease is hyperendemic we conducted a randomized, double-blind, placebo-controlled trial of treatment with a single 200-mg dose of doxycycline in 482 subjects who had removed attached I. scapularis ticks from their bodies within the previous 72 hours. At base line, three weeks, and six weeks, subjects were interviewed and examined, and serum antibody tests were performed, along with blood cultures for Borrelia burgdorferi. Entomologists confirmed the species of the ticks and classified them according to sex, stage, and degree of engorgement. RESULTS Erythema migrans developed at the site of the tick bite in a significantly smaller proportion of the subjects in the doxycycline group than of those in the placebo group (1 of 235 subjects [0.4 percent] vs. 8 of 247 subjects [3.2 percent], P<0.04). The efficacy of treatment was 87 percent (95 percent confidence interval, 25 to 98 percent). Objective extracutaneous signs of Lyme disease did not develop in any subject, and there were no asymptomatic seroconversions. Treatment with doxycycline was associated with more frequent adverse effects (in 30.1 percent of subjects, as compared with 11.1 percent of those assigned to placebo; P<0.001), primarily nausea (15.4 percent vs. 2.6 percent) and vomiting (5.8 percent vs. 1.3 percent). Erythema migrans developed more frequently after untreated bites from nymphal ticks than after bites from adult female ticks (8 of 142 bites [5.6 percent] vs. 0 of 97 bites [0 percent], P=0.02) and particularly after bites from nymphal ticks that were at least partially engorged with blood (8 of 81 bites [9.9 percent], as compared with 0 of 59 bites from unfed, or flat, nymphal ticks [0 percent]; P=0.02). CONCLUSIONS A single 200-mg dose of doxycycline given within 72 hours after an I. scapularis tick bite can prevent the development of Lyme disease.
Obesity Paradox, Obesity Orthodox, and the Metabolic Syndrome: An Approach to Unity

Obesity and its accompanying metabolic syndrome are strongly associated with heightened morbidity and mortality in older adults. In our review of more than 20 epidemiologic studies of major infectious diseases, including tuberculosis, community-acquired pneumonia and sepsis, obesity was associated with better outcomes. A cause-and-effect relationship between over-nutrition and survival with infection is suggested by the results of two preliminary studies of infections in mice, where high-fat feeding for 8–10 wks provided much better outcomes. These better outcomes are reminiscent of many recent studies of “sterile” noninfectious medical and surgical conditions where outcomes for obese patients were better than for their thinner counterparts; this was given the tag “obesity paradox. ” Turning to the history of medicine and biological evolution, we hypothesize that metabolic syndrome has ancient origins and is part of a lifelong metabolic program. While that part of the program promotes morbidity and mortality with aging, it helps infants and children as well as adults fight infections and recover from injuries, key events in the centuries before the public health advances of the 20th century. We conclude with speculation on how an understanding of the biological elements that protect obese patients with infections or injuries might be applied advantageously to thin patients with the same medical challenges.
Nonperturbative QCD coupling and its β function from light-front holography

The light-front holographic mapping of classical gravity in AdS space, modified by a positive-sign dilaton background, leads to a non-perturbative effective coupling $$\alpha_s^{AdS}(Q^2)$$. It agrees with hadron physics data extracted from different observables, such as the effective charge defined by the Bjorken sum rule, as well as with the predictions of models with built-in confinement and lattice simulations. It also displays a transition from perturbative to nonperturbative conformal regimes at a momentum scale $$ \sim 1$$ GeV. The resulting $$\beta$$-function appears to capture the essential characteristics of the full $$\beta$$-function of QCD, thus giving further support to the application of the gauge/gravity duality to the confining dynamics of strongly coupled QCD. Commensurate scale relations relate observables to each other without scheme or scale ambiguity. In this paper we extrapolate these relations to the nonperturbative domain, thus extending the range of predictions based on $$\alpha_s^{AdS}(Q^2)$$.
Presence of repeater F‐waves in the early stage of Guillain‐Barré syndrome

The absence or a prolonged latency of late responses, like F‐waves, is a common neurophysiological finding with diagnostic utility in the early Guillain‐Barré syndrome. However, the presence and the number of repeater F‐waves have not been studied in this disease. In four patients, we report the transient presence of repeater F‐waves in nerves of the lower limbs shortly after the onset of the disease. In each patient, the initial (diagnostic) nerve conduction study showed a high incidence of repeater F‐waves in the tibial or in the peroneal nerves of one side, with normal distal motor latencies; in the other nerves explored the F‐waves were fully abolished and the motor potentials were abnormal. In a second study, done 2–6 weeks later, we observed the abolition of the F‐waves or a significant increase of its minimal latency in those nerves in which we had detected the repeaters. The presence of a high number of repeater F‐waves with normal latencies in some nerves may be a transient and initial electrophysiological sign useful in the early diagnosis of this disease.
Lessons from Basic Research for Pd Treatment

Over the past 30 years, the focus of peritoneal dialysis research has changed from the technical issues related to the establishment of clinical peritoneal dialysis to complex problems of peritoneal membrane biology. Here, we present how these research topics developed, discuss their significance for clinical science, and outline future challenges for peritoneal dialysis research.
A comparison of the effectiveness of lipid-lowering therapy between HIV- and non-HIV-infected subjects with hyperlipidaemia

This retrospective cohort study conducted at the Durham Veterans Affairs Medical Center evaluated the effectiveness and safety of lipid-lowering therapy (LLT) in a HIV-infected population as compared with a general population with hyperlipidaemia. Fifty-three HIV-infected subjects who developed dyslipidaemia and 53 age-matched non-HIV-infected subjects receiving LLT were selected. Efficacy of LLT was assessed after three and six months. Non-HIV-infected subjects were more likely to achieve total cholesterol (TC) goals at three and six months (P = 0.045, P = 0.005) and triglyceride (TG) goals at six months (P = 0.017). Less than 45% of HIV-infected subjects met National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) goals at three or six months. While non-HIV-infected subjects were more likely to achieve TC and TG goals than HIV-infected subjects, overall achievement of NCEP III goals was poor. This result was likely due to treatment with inappropriately low doses of statins.
The Ubiquitin-Proteasome Pathway and Serine Kinase Activity Modulate Adenomatous Polyposis Coli Protein-mediated Regulation of β-Catenin-Lymphocyte Enhancer-binding Factor Signaling*

The tumor suppressor function of the adenomatous polyposis coli protein (APC) depends, in part, on its ability to bind and regulate the multifunctional protein, β-catenin. β-Catenin binds the high mobility group box transcription factors, lymphocyte enhancer-binding factor (LEF) and T-cell factor, to directly regulate gene transcription. Using LEF reporter assays we find that APC-mediated down-regulation of β-catenin-LEF signaling is reversed by proteasomal inhibitors in a dose-dependent manner. APC down-regulates signaling induced by wild type β-catenin but not by the non-ubiquitinatable S37A mutant, β-catenin. Bisindoylmaleimide-type protein kinase C inhibitors, which prevent β-catenin ubiquitination, decrease the ability of APC to down-regulate β-catenin-LEF signaling. All these effects on LEF signaling are paralleled by changes in β-catenin protein levels. Lithium, an inhibitor of glycogen synthase kinase-3β, does not alter the ability of APC to down-regulate β-catenin protein and β-catenin-LEF signaling in the colon cancer cells that were tested. These results point to a role for β-catenin ubiquitination, proteasomal degradation, and potentially a serine kinase other than glycogen synthase kinase-3β in the tumor-suppressive actions of APC.
The relationships between health beliefs and utilization of free health examinations in older people living in a community setting in Taiwan.

OBJECTIVE To examine the influence of demographics and the health beliefs of older people on uptake of a free annual health examination administered by the Twu-Tyan Bureau of Sanitation (TTBS), Pingtung, Taiwan. BACKGROUND The free health examination is a preventive intervention with potential to detect illness. This programme for older people aged 65 years and over, has been available free in some areas since 1980. METHOD A stratified random systematic sample of 200 men and women over 65 years was selected and consisted of 100 individuals who had undertaken the free health examination and 100 who had not. A 17-item Health Belief Scale (HBS) was used to glean information on their health beliefs and uptake of a free health examination. FINDINGS It was found that those with higher education and higher economic status had a greater uptake. Family support was also found to influence take-up. A statistically significant difference was found between the health beliefs of users and nonusers. Users perceived a higher level of seriousness and susceptibility to ill health than nonusers. CONCLUSIONS A number of practical pointers are given that might help clinicians increase uptake rates in such health promotion programmes. These encompass understanding the influence of key demographical and social factors, perceived susceptibility, severity and benefits of the intervention, barriers to attendance and self-efficacy.
Comparison of Users and Non-Users of Mental Health Services Among Depressed Women: A National Study

ABSTRACT Risk factors for major depressive episodes (MDE), determinants of not seeking treatment for it and types of health care service accessed by women who received treatment for MDE were examined using data collected in the Health and Mental Health cycle of the Canadian Community Health Survey, 2002. Of the 1,186 women with MDE, 45% did not receive treatment for it. The highest rates of MDE were found among women under 30 years of age, single mothers with young children, low income women, those with chronic health conditions, high levels of daily stress and low levels of social support. The odds of not seeking treatment for MDE were highest for single mothers with adult children, women with low social support and those with little formal education. The type of mental health services accessed by women with MDE was strongly associated with their age, education, length of time in Canada, language skills, income adequacy, physical health, stress level and chronic health conditions. Our findings signify a need for screening women at high risk of depression, especially those identified as more likely not to seek treatment.
Evaluation of Floating Zone and Epitaxial Planar Silicon Detectors With Different Substrate Thickness After Irradiation up to $2\times 10^{16}$  ${\rm n}_{\rm eq}$ cm$^{- 2}$

Silicon sensors are used for the tracker and vertex detectors of all the main current high energy physics experiments because of their unsurpassed performance in terms of granularity, resolution and speed while offering relatively low mass. The anticipated future upgrade of the present Large Hadron Collider (the Super LHC, sLHC) will require detectors with similar performance in terms of speed and low mass, but with increased granularity and a factor often more radiation tolerance. The radiation hardening of silicon sensors, given the sLHC requirements, is being investigated from many angles: different silicon materials, different electrode geometries and varying the thickness of the active substrate. It has been proposed that possible advantages could be achieved with detectors thinner than the accepted standard of 300 ¿m. The effect of the thickness on the performances of finely segmented silicon sensors after severe hadron irradiation (up to 2 × 1016 1 MeV neutron equivalent/cm2) is presented. For the first time, the charge collection properties of microstrip detectors made on 200 ¿m n-type float zone (FZ) and 150 ¿m high resistivity epitaxial silicon substrates are compared with thin (140 ¿m) and standard p-type devices.
Stored blood transfusion induces transient pulmonary arterial hypertension without impairing coagulation in an ovine model of nontraumatic haemorrhage

Transfusion of blood products in particular older products is associated with patient morbidity. Previously, we demonstrated a higher incidence of acute lung injury in lipopolysaccharide‐treated sheep transfused with stored blood products. As transfusion following haemorrhage is more common, we aimed to determine whether a ‘first hit’ of isolated haemorrhage would precipitate similar detrimental effects following transfusion and also disrupt haemostasis.