Miracle-dz/UniFAR-FTUs huggingface dataset:
Accumulation of apoptotic cells in the epidermis of patients with cutaneous lupus erythematosus after ultraviolet irradiation.

OBJECTIVE To examine whether apoptosis contributes to the pathogenesis of skin lesions in patients with cutaneous lupus erythematosus (CLE) after ultraviolet (UV) irradiation. METHODS In situ nick translation and TUNEL were performed to detect apoptosis in 85 skin biopsy specimens from patients with various subtypes of CLE. Specimens from normal healthy donors and patients with polymorphous light eruption were used as controls. In addition to assessment of primary lesions, provocative phototesting was carried out to investigate events occurring secondary to UV irradiation during a very early stage of lesion formation. RESULTS A significant increase in apoptotic nuclei was found in the upper epidermal layer of primary and UV light-induced skin lesions of CLE patients compared with controls. In tissue sections obtained from control subjects at 24 hours after a single exposure to UV light, a slight increase in the count of epidermal apoptotic nuclei was present as compared with skin tissue from CLE patients obtained under the same conditions before lesion formation. In sections obtained from controls at 72 hours after irradiation, a significant decrease in the apoptotic nuclei count was observed, consistent with a proper clearance of apoptotic cells in the period between 24 and 72 hours after irradiation. In striking contrast, the number of apoptotic nuclei increased significantly within this period in tissue sections from patients with CLE. CONCLUSION These data support the hypothesis that apoptotic cells accumulate in the skin of patients with CLE after UV irradiation, as a result of impaired or delayed clearance. The nonengulfed cells may undergo secondary necrosis and release proinflammatory compounds and potential autoantigens, which may contribute to the inflammatory micromilieu that leads to formation of skin lesions in this disease.
A Novel Phage Element of Salmonella enterica Serovar Enteritidis P125109 Contributes to Accelerated Type III Secretion System 2-Dependent Early Inflammation Kinetics in a Mouse Colitis Model

ABSTRACT Salmonella enterica subsp. I serovar Enteritidis exhibits type III secretion system 2 (TTSS2)-dependent early colonization and inflammation kinetics faster than those of closely related S. enterica serovar Typhimurium. To investigate the accelerated TTSS-2-dependent pathogenic potential of S. Enteritidis, we focused on its genome. Results of a previously published comparative genomic study revealed the presence of mutually exclusive genes in both serovars. In this study, we investigated the roles of six S. Enteritidis-specific genes in vivo by using differential fluorescence induction (DFI) through putative gene-specific promoters. The promoter construct associated with the gene locus SEN1140 induced green fluorescent protein (GFP) expression in the gut lumen, lamina propria, mesenteric lymph nodes, and related systemic organs. To further investigate the potential role of SEN1140, we compared a SEN1140 deletion mutant with S. Typhimurium in a TTSS1-deficient background. Interestingly, the S. Enteritidis mutant lacking SEN1140 did not show the unique TTSS-2-dependent early colonization and inflammation kinetic phenotype of S. Typhimurium. Consistent with this result, complementation of SEN1140 restored the TTSS-2-dependent accelerated inflammatory potential of S. Enteritidis. This report presents a suitable screening strategy that uses a combination of DFI, fluorescence-activated cell sorting, quantitative PCR, and wild-type isogenic tagged-strain techniques to explore the unique roles of S. Enteritidis-specific genes in bacterial pathogenesis.
The Controlled Experiment in the Scientific Method with Special Emphasis on Biological Control

The experimenter questions, manipulates, alters, controls, and the raw untreated soil is an appropriate control-this is the element forces nature to reveal herself. An experiment is a game in which "observed without interference"; but what is the nature of the phenomena are carefully Watched, at a particular time and place, in "peat-bran control" in this case? The sterile peat-bran medium not relation to an intentional disturbance occurring in them and infested with the biological control agent is a candidate. Yet, it is around them. According to Egler (6), "a controlled experiment is not the same substrate as that acted upon by the agent during one where two or more situations are watched, where one is incubation. Further, the addition of previously undigested organic observed without interference, and where the others are matter to soil can induce increased disease severity and/or toxin manipulated in known ways. The changes are then related to this production resulting from the activity of soil microflora leading to known manipulation. " plant damage (15) or, alternatively, can lead to biological control The control is such an integral factor in the scientific method that (2,3). Sterile peat-bran medium has its own "interference" and is it is taken for granted and treated only briefly, if at all, in the not an appropriate control. philosphical literature and is only rarely defined. Indeed, in most Elimination of the biocontrol agent after growth. To allay these disciplines, a control is readily identifiable; for plant pathologists, difficulties, perhaps the agent could be grown in the sterilized however, establishing controls is complicated by the multitude of peat-bran medium and then autoclaved or fumigated to remove the interacting factors in the classic disease triangle. The experiments living portion of the substrate. Much of the nutrient in the peatinvolve pathogens; therefore, there can be inoculated and bran would be utilized by the microorganism prior to the second uninoculated controls. When plant pathologists study biological sterilization; however, the dead cells, stable products extracted by control, they add a new set of parameters (3) involving the the fungus during growth, and any residual unutilized nutrients
Involvement of Caspase-3 in Apoptosis Induced by Viscum album var. coloratum Agglutinin in HL-60 Cells

A cytotoxic lectin (Viscum album L. coloratum agglutinin, VCA) from Korean mistletoe was isolated by affinity chromatography on Sepharose 4B immobilized with asialofetuin. In HL-60 cells, addition of VCA resulted in a dose- and time-dependent growth suppression, morphological changes of apoptotic nuclei, and DNA fragmentation characteristics of apoptosis. To investigate how caspase-3 activation during VCA-induced apoptosis induces cleavages of PARP, the expression of PARP and the pattern of caspase-3 activation in HL-60 cells were investigated. The native and processed PARP forms typically seen in apoptotic cells were observed, and a decrease in expression of the 32-kDa form of caspase-3 in a dose-dependent manner was observed. The VCA-induced apoptosis was significantly inhibited by a caspase-3 specific inhibitor, z-DEVD-FMK, and the PARP processing and caspase-3 activation were also inhibited by the inhibitor. A possible involvement of cell cycle arrest in VCA-induced apoptosis was investigated by flow cytometry and the results suggested that the apoptotic effect of VCA is not involved in the induction of cell cycle arrest.
Altered cortical excitability in obsessive–compulsive disorder

Objective: To assess cortical inhibitory and excitatory mechanisms in obsessive–compulsive disorder (OCD). Background: Transcranial magnetic stimulation (TMS) studies have found decreased neuronal inhibition and a reduced cortical silent period in the primary motor area in Tourette’s syndrome, focal dystonia, and other disorders believed to involve dysfunction of subcortical structures, including the basal ganglia. Dysfunction of the basal ganglia and linked regions also has been implicated in OCD, which has significant clinical and familial overlap with tic disorders. Methods: We applied the TMS techniques previously used in Tourette’s syndrome to a group of 16 OCD patients (seven unmedicated) and 11 age-matched healthy volunteers extensively screened for psychopathology. Measures of motor cortex excitability included resting and active motor threshold, cortical silent period duration, and intracortical inhibition and facilitation using a paired-pulse TMS technique with a subthreshold conditioning stimulus. Results: Similar to recent findings in Tourette’s syndrome and focal dystonia, this study reports significantly decreased intracortical inhibition (ICI) relative to the volunteers at interstimulus intervals from 2 to 5 msec. We also found decreased active and resting motor evoked potential threshold in the OCD patients, another indication of increased cortical excitability. Neither abnormality appeared medication related. The decreases in ICI and motor threshold were greatest in OCD patients with comorbid tics, but remained significant in patients without tics. Conclusions: The data suggest abnormal cortical excitability in obsessive–compulsive disorder. These findings are congruent with the hypothesis that Tourette’s syndrome and obsessive–compulsive disorder (OCD) are analogous disorders with overlapping dysfunction in corticobasal circuits. Patients with tic-related OCD may have more abnormal motor cortex excitability than OCD patients without tics.
Upregulation of mitochondrial peripheral benzodiazepine receptor expression by cytokine‐induced damage of human pancreatic islets

Cytokines produced by immune system cells infiltrating pancreatic islets are candidate mediators of islet beta‐cell destruction in autoimmune insulin‐dependent diabetes mellitus. After 72 h exposure of human pancreatic islets to a cytotoxic cytokine combination of interleukin 1 beta (50 U/ml), tumor necrosis factor alpha (1,000 U/ml), and interferon gamma (1,000 U/ml), an increase of cell death vs. control islets was demonstrated by TUNEL and cell death detection ELISA method. Islet death was associated with apoptosis and mitochondrial swelling as evidenced by electron microscopy. This effect was correlated with a marked decrease of Bcl‐2 mRNA expression (without any major change of Bax mRNA) and a marked increase of inducible nitric oxide synthase mRNA. Since peripheral benzodiazepine receptors constitute the aspecific mitochondrial permeability transition pore, and that it has been suggested to be involved in cytokine‐induced cell death, we evaluated the effects of the cytotoxic cytokines on PBR density and mRNA expression. We demonstrated that cytokine treatment of human islets induced an increase of maximum density of 3H1‐(2‐chlorophenyl‐N‐methyl‐1‐methylpropyl)‐3‐ isoquinolinecarboxamide binding sites, (5,110 ± 193 vs. 3,421 ± 336 fmol/mg proteins, P < 0.05) with no significant change in the affinity constant value (9.45 ± 0.869 vs. 8.7 ± 1.159 nM). Moreover, an increase of the expression of peripheral benzodiazepine receptor mRNA was observed, suggesting an increased transcription from the coding gene. These results suggest a possible role of peripheral benzodiazepine receptors in the organism response to tissue damage associated with inflammatory mediator production. J. Cell. Biochem. 84: 636–644, 2002. © 2001 Wiley‐Liss, Inc.
The Use of Low-Order and Higher-Order Questions in Mathematics Teaching: Video Analyses Case Study

The aim of this video analyses case study was to compare levels of teacher questioning in mathematics classrooms between two countries (United States and Australia) whose language and mode of classroom instruction is English. Data were collected by analysing the video lessons from the United States and Australia in the Learner’s Perspective Study. Descriptive statistics were used to analyse the data. The findings revealed that in both the United States and Australia, male students were more engaged in questions posed by their teachers as compared to the female students. The overall results showed that male students volunteered, and were called on more to teachers’ questions compared to the female students. There were similarities and differences observed and the striking similarity was the frequently asked low-level questions in the lessons. Furthermore, male students were asked more high level and low level questions compared to the female students. In contrast, there were more students in Australia than in the United States who were asked more than two high and low level questions. The data in this study suggest that gender biased teacher questioning do occur in the Year 8 mathematics classes.
Electrocardiographic abnormalities in patients with anorexia nervosa.

Nine patients with anorexia nervosa are described, 7 of whom have abnormal electrocardiograms. These are contrasted with 2 patients with other psychiatric disorders who had also lost weight through failure to eat, and whose electrocardiograms were normal. The abnormalities occurred in the absence of cardiac disease and with normal serum electrolytes. It is postulated that the abnormalities are mediated through stimulation of sympathetic centres within the hypothalamus and release of catecholamines within the myocardium.
[Postoperative wound healing in wound-water contact].

UNLABELLED The avoiding of water-contact to the freshly closed operation-wound is an unwritten law in surgery. In general, taking a bath or shower is not allowed before wound stitch removal under the idea that early water-contact may lead to a higher wound-infection rate. In a prospective trial 170 patients, operated in our short-stay surgery-unit, were allowed to take a shower 24 hours after surgery. Full water contact of the fresh uncovered wound was accepted. The wound-infection rate in this group was compared with the infection-rate of an historical group from our department (n = 956). RESULTS In the water-contact group no case of wound-infection was observed while we were observing a wound-infection-rate of 0.6% in the other group. The difference between the two groups wasn't statistically significant (p = 0.125). CONCLUSION Water-contact of the fresh operation wound 24 hours after surgery did not increase the post-operative wound infection risk in this study.
Executive Personality, Capability Cues, and Risk Taking

We adopt an interactionist logic to study the determinants of risk taking by chief executive officers (CEOs). We introduce the concept of “capability cues”—contextual signals that decision makers might reasonably interpret as indicators of their current level of overall ability—arguing that positive cues will induce boldness, while negative cues will induce timidity. Then, drawing from prior theory about how narcissists react to stimuli, we hypothesize that highly narcissistic CEOs will be relatively unresponsive to objective indicators of their performance; in contrast, highly narcissistic CEOs will be exceptionally emboldened by social praise (in the forms of media praise and media awards). We test our theory in two distinct studies, one of risky outlays by CEOs of publicly owned U.S. companies from 1992 to 2006, and a second of acquisition premiums paid by CEOs of a sample of U.S. acquiring firms, 2001–2008. Our analyses show that capability cues generally influence executive risk taking, but highly narcissistic CEOs are much less responsive to recent objective performance than their less narcissistic peers; in contrast, highly narcissistic CEOs are especially bolstered by social praise.
Concentration of steroids in bovine peripheral plasma during the oestrous cycle and the effect of betamethasone treatment.

Testosterone, oestradiol and progesterone were measured in peripheral plasma during the oestrous cycle of 6 heifers. Oestradiol and progesterone results confirmed earlier reports. Concentration of testosterone on the day of oestrus was 40+/-3 pg/ml (mean+/-S.E.M.), and two peaks were detected during the cycle, one 7 days before oestrus (1809+/-603 pg/ml) and the other (78+/- 7 pg/ml) on the day before the onset of oestrus. The concentration of progesterone declined in most cases 1 day after the maximum concentration of testosterone. Betamethasone treatment in 5 heifers extended luteal function by an average of 10 days: plasma androstenedione and oestradiol concentrations were unaltered; cortisol values were depressed for at least 16 days after treatment; testosterone concentrations were lowered by 13+/-2-4% during treatment, and except in one heifer the peak on Day -7 was abolished.
Quantitative Doppler and Ultrasound Measurements in Surgically Performed Arteriovenous Fistulas of the Arm

Quantitative Doppler flow velocity determinations were made combined with ultrasound estimation of the arterial diameter of the feeding vessel in arteriovenous fistulas of the arm used both for dialysis administration of cytostatics and parenteral nutrition. In 83 patients flow velocity alone was determined while the volume flow was calculated in 63. The effect of the length of the dialysis period and age of the fistula on the different parameters is discussed.
Psychiatric sequelae of abortion. Review of the literature, 1935-1964.

THERAPEUTIC abortion has become a subject of increasing concern for the psychiatrist in the United States. While the rate of therapeutic abortion has decreased over the last 30 years, there has been a marked increase in the relative proportion of psychiatric indications for therapeutic abortion. 1 Since therapeutic abortion raises problems that directly involve religious, political, social, and philosophical values, it is not surprising that the literature takes on the quality of a debate. The purpose of this paper is to examine: (1) the research design, if any, of the reports; (2) the results; and (3) the conclusions drawn from the results. It is our belief that such an appraisal can be useful in planning future studies that will avoid the repetition of errors and direct efforts more fruitfully to elucidating unsolved problems. The primary interest of this review is therapeutic abortion but
Follicular carcinoma of the thyroid gland: prognostic factors, treatment, and survival.

Prognostic variables and treatment outcomes of 82 patients treated at the Northern Israel Oncology Center were reviewed. There were 59 women and 23 men in this series. The female/male ratio was 2.6/1. Median age was 46 years. Median follow-up was 11.4 (range: 3.8-24 years). Median tumor size was 3.6 cm. When first seen, 4 patients had lymph node involvement and 11 (13%) had distant metastases. Surgical treatment was total thyroidectomy in 37 patients (45%), subtotal thyroidectomy in 38 (46%), and lesser procedures in 7 (9%). Sixty-six patients (80%) were treated after surgery with 131I to ablate thyroid remnants. Doses ranged between 30 and 80 mCi. The 20-year overall actuarial survival rate was 65%. The actuarial survival rate of patients <40 years of age was 96% versus 33% in patients >50 years of age (p = 0.0008). Patients with distant metastases at presentation had inferior survival compared with patients without metastases. In conclusion, we found subtotal thyroidectomy followed by 131I and hormone therapy to provide survival similar to that with total thyroidectomy, with less morbidity. Risk factors include: age > or =40 at the time of diagnosis, presence of distant metastases, capsular invasion, tumor size > or =2 cm, and male gender.
Variants in estrogen‐related genes and risk of Parkinson's disease

Incidence rates of Parkinson's disease are higher in men than in women at all ages, and these differences may be a result of the neuroprotective effects of estrogen on the nigrostriatal pathway. We investigated the association of common variants in 4 estrogen‐related genes with Parkinson's disease. Tagging single‐nucleotide polymorphisms in the CYP19A1, ESR1, ESR2, and PRDM2 genes were selected from the International Haplotype Map and genotyped in 1103 Parkinson's disease cases from the upper Midwest of the United States and in 1103 individually matched controls (654 unaffected siblings, and 449 unrelated controls from the same region). Of 137 informative single‐nucleotide polymorphisms, 2 PRDM2 single‐nucleotide polymorphisms were significantly associated with an increased risk of Parkinson's disease at the Bonferroni‐corrected significance level of 0.0004 (rs2744690: OR, 1.54; SE(logOR), . 109; 99.96% CI, 1.05–2.26; uncorrected P = . 0001; rs2744687: OR, 1.53; SE(logOR), . 113; 99.96% CI, 1.03–2.29, uncorrected P = . 0002); the association was significant in the women‐only stratum but not in the men‐only stratum. An additional 6 single‐nucleotide polymorphisms in PRDM2, 2 in ESR1, 1 in ESR2, and 1 in CYP19A1 had significant P values in the overall sample before Bonferroni correction. None of the single‐nucleotide polymorphisms were significantly associated with age at onset of Parkinson's disease after Bonferroni correction. Our results confirm the association of PRDM2 variants with Parkinson's disease susceptibility, especially in women. © 2011 Movement Disorder Society
Chinese Gini Coefficient from 2005 to 2012, Based on 20 Grouped Income Data Sets of Urban and Rural Residents

Data insufficiency has become the primary factor affecting research on income disparity in China. To resolve this issue, this paper explores Chinese income distribution and income inequality using distribution functions. First, it examines 20 sets of grouped data on family income between 2005 and 2012 by the China Yearbook of Household Surveys, 2013, and compares the fitting effects of eight distribution functions. The results show that the generalized beta distribution of the second kind has a high fitting to the income distribution of urban and rural residents in China. Next, these results are used to calculate the Chinese Gini ratio, which is then compared with the findings of relevant studies. Finally, this paper discusses the influence of urbanization on income inequality in China and suggests that accelerating urbanization can play an important role in narrowing the income gap of Chinese residents.
Asthma, Airway Symptoms and Rhinitis in Office Workers in Malaysia: Associations with House Dust Mite (HDM) Allergy, Cat Allergy and Levels of House Dust Mite Allergens in Office Dust

A prevalence study was conducted among office workers in Malaysia (N= 695). The aim of this study was to examine associations between asthma, airway symptoms, rhinitis and house dust mites (HDM) and cat allergy and HDM levels in office dust. Medical data was collected by a questionnaire. Skin prick tests were performed for HDM allergens (Dermatophagoides pteronyssinus, Dermatophagoides farinae) and cat allergen Felis domesticus. Indoor temperature and relative air humidity (RH) were measured in the offices and vacuumed dust samples were analyzed for HDM allergens. The prevalence of D. pteronyssinus, D. farinae and cat allergy were 50.3%, 49.0% and 25.5% respectively. Totally 9.6% had doctor-diagnosed asthma, 15.5% had current wheeze and 53.0% had current rhinitis. The Der p 1 (from D. pteronyssinus) and Der f 1 (from D. farinae) allergens levels in dust were 556 ng/g and 658 ng/g respectively. Statistical analysis was conducted by multilevel logistic regression, adjusting for age, gender, current smoking, HDM or cat allergy, home dampness and recent indoor painting at home. Office workers with HDM allergy had more wheeze (p= 0.035), any airway symptoms (p= 0.032), doctor-diagnosed asthma (p= 0.005), current asthma (p= 0.007), current rhinitis (p= 0.021) and rhinoconjuctivitis (p< 0.001). Cat allergy was associated with wheeze (p= 0.021), wheeze when not having a cold (p= 0.033), any airway symptoms (p= 0.034), doctor-diagnosed asthma (p= 0.010), current asthma (p= 0.020) and nasal allergy medication (p= 0.042). Der f 1 level in dust was associated with daytime breathlessness (p= 0.033) especially among those with HDM allergy. Der f 1 levels were correlated with indoor temperature (p< 0.001) and inversely correlated with RH (p< 0.001). In conclusion, HDM and cat allergies were common and independently associated with asthma, airway symptoms and rhinitis. Der f 1 allergen can be a risk factor for daytime breathlessness.