Miracle-dz/UniFAR-FTUs huggingface dataset:
The mechanism of error induction by the antibiotic viomycin provides insight into the fidelity mechanism of translation

Applying pre-steady state kinetics to an Escherichia-coli-based reconstituted translation system, we have studied how the antibiotic viomycin affects the accuracy of genetic code reading. We find that viomycin binds to translating ribosomes associated with a ternary complex (TC) consisting of elongation factor Tu (EF-Tu), aminoacyl tRNA and GTP, and locks the otherwise dynamically flipping monitoring bases A1492 and A1493 into their active conformation. This effectively prevents dissociation of near- and non-cognate TCs from the ribosome, thereby enhancing errors in initial selection. Moreover, viomycin shuts down proofreading-based error correction. Our results imply a mechanism in which the accuracy of initial selection is achieved by larger backward rate constants toward TC dissociation rather than by a smaller rate constant for GTP hydrolysis for near- and non-cognate TCs. Additionally, our results demonstrate that translocation inhibition, rather than error induction, is the major cause of cell growth inhibition by viomycin.
Postoperative Delirium and Postoperative Cognitive Dysfunction: Overlap and Divergence.

WHAT WE ALREADY KNOW ABOUT THIS TOPIC Postoperative delirium and postoperative cognitive dysfunction both occur in a substantial number of older surgical patientsPostoperative delirium and postoperative cognitive dysfunction share risk factors and may co-occur, although their relationship is unclear WHAT THIS ARTICLE TELLS US THAT IS NEW: Postoperative delirium increased the risk of postoperative cognitive dysfunction at 1 month postoperatively but there was no association between postoperative delirium and cognitive dysfunction at 2 and 6 months after major noncardiac surgeryPostoperative delirium and longer-term postoperative cognitive dysfunction may be different disorders BACKGROUND:: Postoperative delirium and postoperative cognitive dysfunction share risk factors and may co-occur, but their relationship is not well established. The primary goals of this study were to describe the prevalence of postoperative cognitive dysfunction and to investigate its association with in-hospital delirium. The authors hypothesized that delirium would be a significant risk factor for postoperative cognitive dysfunction during follow-up. METHODS This study used data from an observational study of cognitive outcomes after major noncardiac surgery, the Successful Aging after Elective Surgery study. Postoperative delirium was evaluated each hospital day with confusion assessment method-based interviews supplemented by chart reviews. Postoperative cognitive dysfunction was determined using methods adapted from the International Study of Postoperative Cognitive Dysfunction. Associations between delirium and postoperative cognitive dysfunction were examined at 1, 2, and 6 months. RESULTS One hundred thirty-four of 560 participants (24%) developed delirium during hospitalization. Slightly fewer than half (47%, 256 of 548) met the International Study of Postoperative Cognitive Dysfunction-defined threshold for postoperative cognitive dysfunction at 1 month, but this proportion decreased at 2 months (23%, 123 of 536) and 6 months (16%, 85 of 528). At each follow-up, the level of agreement between delirium and postoperative cognitive dysfunction was poor (kappa less than .08) and correlations were small (r less than .16). The relative risk of postoperative cognitive dysfunction was significantly elevated for patients with a history of postoperative delirium at 1 month (relative risk = 1.34; 95% CI, 1.07-1.67), but not 2 months (relative risk = 1.08; 95% CI, 0.72-1.64), or 6 months (relative risk = 1.21; 95% CI, 0.71-2.09). CONCLUSIONS Delirium significantly increased the risk of postoperative cognitive dysfunction in the first postoperative month; this relationship did not hold in longer-term follow-up. At each evaluation, postoperative cognitive dysfunction was more common among patients without delirium. Postoperative delirium and postoperative cognitive dysfunction may be distinct manifestations of perioperative neurocognitive deficits.
Bax-Dependent Spermatogonia Apoptosis Is Required for Testicular Development and Spermatogenesis1

Abstract Bax is a multidomain, proapoptotic member of the Bcl-2 family that is required for normal spermatogenesis in mice. Despite its proapoptotic function, previous results found that Bax-deficient mature male mice demonstrate increased cell death and dramatic testicular atrophy. The present study examined the role of Bax during the normal development of the testis to determine whether the increased cell death in mature mice could be explained by decreased apoptosis earlier in development. Consistent with this hypothesis, testicular atrophy is preceded by increased testicular weight and hypercellular tubules in immature Bax-deficient mice. TUNEL staining at Postnatal Day (P) 7 and morphological quantitation between P5 and P15 demonstrates decreased germ cell apoptosis in Bax-deficient mice. By P15, increased numbers of type A spermatogonia, and at P12 and P15, an increase in intermediate type spermatogonia were noted in Bax-deficient animals. By P25, the number of basal compartment cells was greatly increased in Bax-deficient animals compared with controls such that four or five layers of preleptotene spermatocytes were routinely present within the basal compartment of the testis. Although the Sertoli cell barrier was significantly removed from the basement membrane, it appeared intact as judged by the hypertonic fixation test. During late pubertal development, massive degeneration of germ cells took place, including many of those cell types that previously survived in the first wave of spermatogenesis. The data indicate that Bax is required for normal developmental germ cell death in the type A spermatogonia, specifically dividing (A2, A3, and A4) spermatogonia, at a time at which the number of spermatogonia is regulated in a density-dependent manner. The massive hyperplasia that occurs in Bax-deficient mice subsequently results in Bax independent cell death that may be triggered by overcrowding of the seminiferous epithelium.
Susceptibilities of Herpes Simplex Viruses to Penciclovir and Acyclovir in Eight Cell Lines

ABSTRACT The commonly used antiviral drugs acyclovir (ACV) and penciclovir (PCV) possess similarly potent antiviral activities in vivo against herpes simplex virus (HSV). Assay methods for sensitivity to ACV are not necessarily transferable to PCV, even though the two drugs have similar in vivo potencies and mechanisms of action. We determined by plaque reduction assay the relative activities of ACV and PCV against five laboratory-adapted strains of HSV types 1 and 2 (including sensitive and resistant strains) in seven human cell lines and one nonhuman primate cell line. Seven characteristics were used to evaluate the cell lines. All cell lines were similar in their plating efficiencies and abilities to discriminate between sensitive and resistant HSV isolates. Vero and MRC-5 cells yielded the most discordant 50% inhibitory concentrations (IC50s) for the two HSV types, while Vero and WI-38 VA-13 cells yielded large differences in the IC50s of ACV and PCV. The limited life spans and poor plaque morphologies of the fibroblast lines were undesirable characteristics. Among the transformed cell lines producing well-defined plaques, A549 cells provided the best concordance between IC50s for the two HSV types and two antiherpes drugs. Comparison experiments with a yield reduction format indicated that the use of assays of this type might allow some of the cell-specific properties observed in plaque reduction assays to be avoided.
Modeling fatal traffic accident occurrences in small Indian cities, Patiala, and Rajpura

Abstract This article investigates the effects of highway-related correlates such as geometric design features and traffic factors on fatal accident occurrences in small-sized Indian cities, Patiala and Rajpura, by estimating Negative Binomial regression models. Between 2013 and 2015, 61 and 188 fatal accidents were recorded in Rajpura and Patiala, respectively. The study reveals that the number of traffic lanes positively influences accident occurrences in Rajpura. On the other hand, in Patiala, the width of paved road surface, segment length and average daily traffic (ADT) are positively correlated with accident rates. Intercity highways passing through rural areas near the city boundary are more prone to fatal accidents than the highway segments within urban limits. Elasticity analysis suggests that an increase in paved road width and ADT by one percent would increase accident rates by 0.7% and 0.436% in Patiala and Rajpura, respectively. The findings of this research concur with the results of previous studies that infrastructural improvements such as increasing the number of lanes and paved road width are positively correlated with accident rates. It is expected that understanding the association between accident rates and non-behavioral factors would help transportation engineers identify the deficiencies in roadway designs in the study cities.
Femininity, Masculinity and Fear of Crime within Heterosexual R

Introduction Fear of crime, or concern about the likelihood of criminal victimisation, has constituted an area of criminological enquiry for over thirty years (Warr and Ellison, 2000, p.551). Since the earliest victim surveys (McLaughlin, 2001, p.165), repeated studies have concluded that fear of crime, be it rational or irrational (Lupton and Tulloch, 1999, p.507), is an issue that must be taken seriously. Indeed, as Gilchrist et al. point out:
Lipidomic Signatures Reveal Seasonal Shifts on the Relative Abundance of High-Valued Lipids from the Brown Algae Fucus vesiculosus

Fucus vesiculosus is an edible brown macroalga, with health benefits associated with its consumption and also a source of bioactive molecules. It is acknowledged that the biochemical composition of macroalgae changes when exposed to different environmental conditions occurring on different habitats, such as the water temperature, and light intensity. In the present study, the polar lipidome of Fucus vesiculosus was characterized for the first time using modern high-resolution HILIC–MS, and MS/MS approaches, to evaluate the phenotypic variability in two seasons of the year, e.g., winter and spring. A total of 187 molecular species were identified over eighteen classes of glycolipids, phospholipids and betaine lipids. Principal component analysis (PCA) multivariate statistical analysis and cluster analysis of polar lipid classes, polar lipid species and total fatty acids (FA) datasets, showed clustering according to the seasonal groups. While the lipid profile of Fucus vesiculosus harvested in the winter and spring yielded the same molecular species, the relative abundance of these species was significantly different. In the winter, changes were mainly due to the increased relative abundance of some molecular species of glycolipids and phospholipids, bearing octadeca(poly)enoic (18:3, 18:4) and eicosa(poly)enoic (20:4, 20:5) FA and betaine lipids species with short saturated FA (14:0) and polyunsaturated FA (PUFA). Importantly, glycolipids with n-3 PUFA and sulfolipids, have been reported to have important biological activities and therapeutic value. Overall, Fucus vesiculosus is a promising source of bioactive compounds that can be used as functional food or ingredients for human nutrition, feed, pharma, and cosmetic formulations. In this study, samples harvested in the winter season maximized yields of these bioactive components, when compared with samples harvested in the spring.
Antimicrobial peptide LL-37 is bactericidal against Staphylococcus aureus biofilms

Our current challenge in the management of prosthetic joint infection is the eradication of biofilms which has driven the need for improved antimicrobial agents and regimens. In this study, the antimicrobial peptide, LL-37, and silver nanoparticles (AgNPs) were investigated for their antimicrobial efficacies against Staphylococcus aureus (S. aureus), a microorganism commonly implicated in biofilm-related infections. These antimicrobials were compared to conventional antibiotics and combination treatments with rifampin. Using a Centers for Disease Control reactor, 24 h S. aureus biofilms were formed on cobalt-chromium discs and the anti-biofilm activity was determined by quantifying the amount of colony forming units following treatments. We found that LL-37 was the most efficacious antimicrobial agent with a more than 4 log reduction in colony counts. In comparison, silver nanoparticles and conventional antibiotics were not as efficacious, with a less than 1 log reduction in colony counts. Antimicrobial combination treatments with rifampin significantly increased the log reduction for AgNPs and gentamicin, although still significantly less than LL-37 in isolation. Furthermore, kinetic studies revealed the rapid elimination of S. aureus biofilm with LL-37. Collectively, the results of this study demonstrated that LL-37 was an effective agent against S. aureus biofilms and may have potential clinical applications in the eradication of biofilms and treatment of prosthetic joint infection.
Taking a break: The effect of taking a vacation from Facebook and Instagram on subjective well-being

Social Networking Sites (SNS) such as Facebook and Instagram have relocated a large portion of people’s social lives online, but can be intrusive and create social disturbances. Many people therefore consider taking an “SNS vacation. ” We investigated the effects of a one-week vacation from both Facebook and Instagram on subjective well-being, and whether this would vary for passive or active SNS users. Usage amount was measured objectively, using RescueTime software, to circumvent issues of self-report. Usage style was identified at pre-test, and SNS users with a more active or more passive usage style were assigned in equal numbers to the conditions of one-week SNS vacation (n = 40) or no SNS vacation (n = 38). Subjective well-being (life satisfaction, positive affect, and negative affect) was measured before and after the vacation period. At pre-test, more active SNS use was found to correlate positively with life satisfaction and positive affect, whereas more passive SNS use correlated positively with life satisfaction, but not positive affect. Surprisingly, at post-test the SNS vacation resulted in lower positive affect for active users and had no significant effects for passive users. This result is contrary to popular expectation, and indicates that SNS usage can be beneficial for active users. We suggest that SNS users should be educated in the benefits of an active usage style and that future research should consider the possibility of SNS addiction among more active users.
The Impact of Game Outcome on Affect of Military Wheelchair Basketball Players.

Participation in physical activity has been shown to improve components of psychological well-being (i.e., affect). Programs such as the Warrior Games have been designed to promote physical activity in wounded military personnel. However, sport competition typically yields a winner and a loser (i.e., game outcome). The experience of a win or a loss may affect how wounded athletes respond to game outcome. Therefore, the purpose of this study was to investigate the affective changes (positive affect, negative affect, tranquility, and fatigue) according to game outcome in a sample of wounded military wheelchair basketball players participating in a weekend tournament. The results indicated that the participants who experienced a win reported significantly higher positive affect and tranquility and significantly lower negative affect than those experiencing a loss. These findings have important implications for wounded veteran athletes, as well as coaches and administrative personnel.
Referral and care for acute ischemic stroke in a Japanese tertiary emergency hospital

To examine the current emergency referral and care for acute stroke at a Japanese tertiary emergency hospital with a 24‐h stroke team and care unit, we surveyed the presentations of patients with acute ischemic stroke or transient ischemic attack (TIA) seen within 7 days of onset. Delay from symptom onset to arrival at our hospital, from arrival to initial diagnostic brain computed tomography (CT), and the type of anti‐thrombotic treatments were evaluated. During the 18‐month period, there were 254 ischemic events in 244 patients; 239 (94%) had an ischemic stroke and 15 (6%) TIA. Eighty‐two (32%) events presented within 3 h of onset, and 102 (40%) and 179 (70%) within the first 6 and 24 h, respectively. The median delay from hospital arrival to CT was 32 min, ranging 10 min to 22 h. Two hundred (79%) events underwent CT within 1 h of arrival (n=172) or at the referral hospitals before transfer (n=28). Direct ambulance transportation and more severe neurological deficits were independent predictors both for early arrival and short in‐hospital delay to CT. Anti‐thrombotic therapies including anticoagulant and/or antiplatelet medications were given in 237 (93%) episodes. Two (1%) patients received thrombolysis, although 18 (7%) patients fulfilled the National Institute of Neurological Disorders and Stroke guidelines for intravenous thrombolysis with tissue plasminogen activator. As in western communities, our pre‐hospital emergency referral systems for acute stroke require substantial improvements including the wider use of ambulance calling. Although our in‐hospital stroke management is functioning relatively well, further efforts are necessary in reducing the diagnostic delay.
The association between caffeinated alcoholic beverages and the perpetration of intimate partner violence

ABSTRACT Background: Problematic alcohol use is a recognized risk factor for intimate partner violence (IPV) perpetration.Objective: The use of caffeinated alcoholic beverages (CAB) appears to be associated with high-risk drinking behavior but the relationship between CAB use and IPV has yet to be explored.Methods: Sixty male and 40 female married or dating participants responded to an online survey including measures of past-year alcohol use and partner violence.Results: Logistic regression analyses revealed that CAB users were significantly more likely to perpetrate physical assault and partner injury after controlling for age, sex, ethnicity, income, and heavy alcohol use. The relationship between CAB use and perpetration of sexual coercion was better accounted for by heavy alcohol use.Conclusion: Results from the current study suggest that there exists an independent link between CAB use and partner violence perpetration.
Evaluation of implementation of enterprise resource planning information system with DeLone and McLean model approach

This study aimed to examine and analyze the variables that influence the implementation of Enterprise Resource Planning Information Systems at PT. Telkom Indonesia Tbk. This study used DeLone and McLean Information Systems Success Models (D&M IS Success Model), in which the success of information systems was proxied by user satisfaction. While the variables that influence the success of information system tested in this study were system quality, information quality and service quality. In addition, this study also tested and analyzed the effect of information systems success on the net benefits. This research was causal (conclusive). The data collection was conducted by distributing questionnaires to users of ERP SAP in PT Telkom for 189 people consisting of 116 finance and controlling modules users, 16 of human resources modules users and 57 of materials management module users. Data processing method was performed by Structural Equation Modelling (SEM) with partial least square (PLS) approach by using Smart PLS 3.0 software. In this study, it was identified that the user satisfaction of Enterprise Resources Planning (ERP) information systems in this model can be explained by the quality system, the quality of information and the service quality by 60.5% in the order from the greatest influence was the quality of information (path 0.361), the system quality (path 0,260) and the last was the variable of service quality (path 0,253). In addition, the net benefits of the implementation of Enterprise Resources Planning (ERP) information systems, both the benefits for individual's user as well as for the enterprise can be explained by the user satisfaction variable for 61.8%.
Putting its fingers on stressful situations: the heavy metal‐regulatory transcription factor MTF‐1

It has been suggested that metallothioneins, discovered about 45 years ago, play a central role in heavy metal metabolism and detoxification, and in the management of various forms of stress. The metal‐regulatory transcription factor‐1 (MTF‐1) was shown to be essential for basal and heavy metal‐induced transcription of the stress‐responsive metallothionein‐I and metallothionein‐II. Recently it has become obvious that MTF‐1 has further roles in the transcriptional regulation of genes induced by various stressors and might even contribute to some aspects of malignant cell growth. Furthermore, MTF‐1 is an essential gene, as mice null‐mutant for MTF‐1 die in utero due to liver degeneration. We describe here the state of knowledge on the complex activation of MTF‐1, and propose a model with MTF‐1 as an interconnected cellular stress‐sensor protein involved in heavy metal metabolism, hepatocyte differentiation and detoxification of toxic agents. BioEssays 23:1010–1017, 2001. © 2001 John Wiley & Sons, Inc.
Association of serum gastric inhibitory polypeptide and pancreatic polypeptide levels with prolonged esophageal acid exposure time in refractory gastroesophageal reflux disease

Abstract Background: Acid exposure time (AET) prolongation plays an important role in the pathogenesis of gastroesophageal reflux disease (GERD). Gastric inhibitory polypeptide (GIP) and pancreatic polypeptide (PP) participate in the regulation of gastric acid secretion, blood glucose and lipid levels, and food intake. In this study, we evaluated the serum GIP and PP levels in refractory GERD patients and analyzed their metabolic and motility characteristics. Methods: Seventy-three refractory GERD patients were enrolled in this study from September 2015 to September 2017. We investigated the clinical characteristics, severity, and duration of GERD symptoms. High-resolution manometry and 24 hours impedance-pH monitoring were performed to assess esophageal motility and reflux parameters. The patients were divided into the AET− group (AET <4.2%) and AET+ group (AET >4.2%). GIP and PP levels were determined in all subjects and their associations with other parameters evaluated. Results: Age and GERDQ score were significantly higher (P < .05) and acid reflux and heartburn more frequent in the AET+ group than in the AET− group. The contraction front velocity was increased in the AET− group, while there was no significant difference in the distal contraction integral, peristalsis interruption, distal latency, or resting pressures of the lower and upper esophageal sphincters between the 2 groups (P > .05). The serum levels of GIP (P = .003) and PP (P = .012) were significantly increased in the AET+ group. Increased GIP and PP levels were associated with abnormal upright AET (correlation coefficients 0.307 and 0.233, P = .008 and P = .047). There was a positive correlation between GIP and triglyceride levels (correlation coefficient 0.279, P = .017). Conclusion: The serum levels of GIP and PP in refractory GERD patients with prolongation of AET are significantly elevated, mainly in the upright position.