Miracle-dz/UniFAR-FTUs huggingface dataset:
Complex permittivity of low loss thermoplastic composites using a resonant cavity method

The use of high energy rate joining of fibre-reinforced thermoplastic (FRTP) composites using microwaves has yielded promising preliminary results and more research is being carried out so that the technology can find its application in manufacturing industries shortly. Methods such as the dielectric probe and waveguide transmission have been successfully used to measure the dielectric constant (e') of low loss thermoplastic composites but they cannot give reliable results when used to measure the dielectric loss (e''). This paper describes a convenient laboratory based method designed to obtain e', e'' and hence loss tangent tan forlow loss thermoplastic composite materials like glass fibre reinforced (33%) low density polyethylene [LDPE/GF(33%)]. The method is called the resonant cavity method and the schematic diagram of the set up and that of the cavity is depicted in Fig.1 and Fig. 2 respectively.
Validity of breathing frequency to monitor exercise intensity in trained cyclists.

This study compared breathing frequency (fB) during a ventilation threshold (VT) test with fB during a simulated 40 km time trial (40TT) to assess whether fB can be used to monitor exercise intensity. Twenty-six male cyclists (mean +/- SD VO2max = 4.42 +/- 0274 l.min-1) performed an incremental VT ergometer test and a simulated 40TT on a set of wind-loaded rollers. During the 40TT, expired gas was collected for 3 min using open circuit spirometry at 10 km intervals. A significant correlation (r = 0.89; p < or = 0.05) was found between the fB threshold (VTf) and the gas exchange VT (VTg) (3.42 +/- 0.08 and 3.31 +/- 0.09 l.min-1, respectively). No differences were found in VO2 at the VTf vs. X VO2 (3.48 +/- 0.08 l.min-1) during the simulated 40TT. However, when fB in br.min-1 calculated from the VT test (32.5 br.min-1), was compared with fB during the 40TT (45.6 br.min-1), there was a significant difference. Ventilation, heart rate and the respiratory exchange ratio were all significantly higher during the 40TT. Although a significant relationship was found between the VT test and the 40TT for VTf measured in l.min-1 of VO2 (r = 0.66; p < or = 0.05), fB in br.min-1 obtained from the incremental test should not be used to identify exercise training intensity. These data suggest that during competition, trained athletes exercise at a higher fB than that determined when being tested, implying that fB in br.min-1 may not be a valid measurement of exercise intensity.
Evoked potential recording during echolocation in a false killer whale Pseudorca crassidens.

Auditory brainstem responses (ABRs) were recorded in a false killer whale while the animal echolocated a target. The ABR collection was triggered by echolocation clicks of the animal. In these conditions, the recorded ABR pattern contained a duplicate set of waves. A comparison of ABR wave delays recorded during echolocation with those recorded during regular external stimulation with experimenter generated clicks showed that the first set of waves may be a response to the emitted click whereas the second one may be a response to the echo. Both responses, to the emitted click and to the echo, were of comparable amplitude in spite of the intensity difference of these two sounds that may differ by more than 40 dB near the animal’s head. This finding indicates the presence of some mechanism of releasing responses to echoes from masking by loud emitted clicks. The evoked-potential method may be productive to investigate these mechanisms.
Influence of randomness in mathematical models for diffraction and T-cell recognition

Stochastic models play an important part in many fields of science like physics and biology. In this thesis the influence of randomness in two such models is considered. It is therefore divided into two parts. The first part deals with diffraction. Starting from a deterministic model we analyze the influence of randomness. The main result is the proof of the absence of singular continuous parts in the diffraction measure of particle gases with short range interaction. The second part deals with recognition in the immune system. In this part the main focus lies in the probabilistic modeling itself, preparation of the mathematical tools, and the analysis to show the ability of the models to explain the reliable recognition of foreign invaders by certain white blood cells.
Experimental scleroma

Klebsilla rhinoscleromatis suspension was experimentally inoculated into the nose and maxillary sinus of eight adult healthy rabbits. Two normal litter-mates served as controls. The animals were killed at varying intervals up to five months following the inoculation. Histopathological examination of the upper and lower respiratory tracts revealed a consistent pulmonary peribronchial and perivascular granulomatous reaction with a Mikulic cell-like appearance. The reaction was present in all the inoculated rabbits and increased in severity as the survival period was prolonged. The significance of these findings in the aetiopathogenesis of scleroma and to scleroma in humans is discussed.
Identification of a Calcium/Calmodulin-dependent Protein Kinase That Phosphorylates the Neurospora Circadian Clock Protein FREQUENCY*

Phosphorylation of circadian clock proteins represents a major regulatory step that controls circadian clocks. InNeurospora, the circadian clock protein FREQUENCY (FRQ) is progressively phosphorylated over time, and its level decreases when it is hyperphosphorylated. In this study, we showed that most of the kinase activity phosphorylating FRQ in vitro was calcium/calmodulin-dependent, and the endogenous FRQ in theNeurospora extracts was phosphorylated by a Ca/CaM-dependent kinase-like activity. FromNeurospora cell extracts, an ∼50-kDa Ca/CaM-dependent kinase (CAMK-1) that can specifically phosphorylate FRQ was purified. In vitro, this kinase accounts for near half of the FRQ kinase activity, and it can phosphorylate the FRQ region that contains the three known functionally important phosphorylation sites. To understand the function of camk-1 in vivo, it was disrupted inNeurospora by gene replacement. After germination from ascospores, the camk-1 null strains grew slowly, indicating that CAMK-1 plays an important role in growth and development ofNeurospora. This phenotype was transient however, revealing redundancy in the system. Analysis of the camk-1 null strain revealed that the deletion of camk-1 affected phase, period, and light-induced phase shifting of the circadian conidiation rhythm. Taken together, our results suggest that multiple kinases may phosphorylate FRQ in vivo.
Differential Control of Clathrin Subunit Dynamics Measured with EW‐FRAP Microscopy

The clathrin triskelion is composed of three light chain (LC) and three heavy chain (HC) subunits. Cellular control of clathrin function is thought to be aimed at the LC subunit, mainly on the basis of structural information. To test this hypothesis in vivo, we used evanescent‐wave photobleaching recovery to study clathrin exchange from single pits using LC (LCa and LCb) and HC enhanced green fluorescent protein fusion constructs. The recovery signal was corrected for cytosolic diffusional background, yielding the pure exchange reaction times. For LCa, we measured an unbinding time constant τLEa = 18.9 ± 1.0 seconds at room temperature, faster than previously published; for LCb, we found τLCb = 10.6 ± 1.9 seconds and for HC τHC = 15.9 ± 1.0 seconds. Sucrose treatment, ATP or Ca2+ depletion blocked exchange of LCa completely, but only partially of HC, lowering its time constant to τ = 10.0 ± 0.9 seconds, identical to the one for LCb exchange. The latter was also not blocked by Ca2+ depletion or sucrose. We conclude that HCs bound both to LCa and to LCb contribute side by side to pit formation in vivo, but the affinity of LCa‐free HC in pits is reduced, and the Ca2+‐ and ATP‐mediated control of clathrin function is lost.
Postprint Exploring the two-gene ratio in breast cancer – independent roles for HOXB 13 and IL 17 BR in prediction of clinical outcome

Background: The two-gene expression ratio HOXB13:IL17BR has been proposed to predict the outcome of tamoxifen-treated breast cancer patients. We intended to examine whether this ratio can predict the benefit of 5 years vs. 2 years of tamoxifen treatment of postmenopausal patients. A further objective was to investigate any prognostic effects of the ratio in systemically untreated premenopausal patients. Based on the current knowledge of HOXB13 and IL17BR, we hypothesized that these genes may have individual prognostic or predictive power. Patients and methods: Expression of HOXB13 and IL17BR were quantified by real-time PCR in tumors from 264 randomized postmenopausal patients and 93 systemically untreated premenopausal patients. Results: A high HOXB13:IL17BR ratio was associated with aggressive tumor characteristics, as were low levels of IL17BR alone. The ratio and HOXB13 alone predicted recurrence-free survival after endocrine treatment, with a benefit of prolonged treatment in estrogen receptorpositive patients correlated to a low ratio (recurrence rate ratio: RR=0.39; p=0.030), or low expression of HOXB13 (RR=0.37; p=0.015). No difference in recurrence-free survival was seen for the high ratio or high HOXB13 subgroups. The predictive value of HOXB13 and HOXB13:IL17BR was significant in multivariate analysis. In the systemically untreated cohort, only IL17BR showed independent prognostic significance. Conclusion: We conclude that the ratio or HOXB13 alone can predict the benefit of endocrine therapy, with a high ratio or a high expression rendering patients less likely to respond. We have also shown that IL17BR might be an independent prognostic factor in breast cancer.
Unpacking Student Feedback as a Basis for Metacognition and Mediated Learning Experiences: A Socio-cultural perspective

The study examines the effects of the feedback given to students by lecturers as learning support. It was conducted with undergraduates in an educational theory course in a South African university. The thesis was that although some of the feedback messages transmitted to students regarding strengths and weaknesses in learning get easily decoded and turned into action to improve performance, some messages are misconstrued by the students making the process of giving feedback complex. Data was collected through a cross-sectional feedback survey utilizing focus group interviews with 50 Bachelor of Education pre-service students conveniently sampled. The data analysis followed a thematic approach with superordinate themes used to structure the discussion of findings. The study found that student feedback needs to be culturally responsive for it to foster metacognition in them. The conclusion was that unless lecturers provide feedback that is simple, meaningful and clearly focused, students are unlikely to take much heed of it as there is a general tendency to focus more on the marks obtained than on the role of the feedback provided. This makes some students leave university under-prepared or half-baked in terms of providing student feedback on performance.
On exponential bounds on the Bayes risk of the kernel classification rule

The exponential, distribution-free bounds for the kernel classification rule are derived. The equivalence of all modes of the global convergence of the rule is established under optimal assumptions on the smoothing sequence. Also derived is the optimal global rate of convergence of the kernel regression estimate within the class of Lipschitz distributions. The rate is optimal for the nonparametric regression, but not for classifications. It is shown. using the martingale device, that weak, strong, and complete L/sub 1/ Bayes risk consistencies are equivalent. Consequently the conditions on the smoothing sequence h/sub n/ to 0 and nh/sub n/ to infinity are necessary and sufficient for Bayes risk consistency of the kernel classification rule. The rate of convergence of the kernel classification rule is also given. >
A New Aspergillus fumigatus Resistance Mechanism Conferring In Vitro Cross-Resistance to Azole Antifungals Involves a Combination of cyp51A Alterations

ABSTRACT Fourteen Aspergillus fumigatus clinical isolates that exhibited a pattern of reduced susceptibility to triazole drugs were analyzed. The sequences of the cyp51A gene from all isolates showed the presence of a point mutation at t364a, which led to the substitution of leucine 98 for histidine (L98H), together with the presence of two copies of a 34-bp sequence in tandem in the promoter of the cyp51A gene. Quantitative expression analysis (real-time PCR) showed up to an eightfold increase in the level of expression of the cyp51A gene compared to that by the susceptible strain. Three PCR fragments of one azole-resistant strain (strain CM2627) that included the promoter with the tandem repeat and part of cyp51A with the t364a mutation or PCR fragments with only one of the modifications were used to replace the cyp51A gene of an azole drug-susceptible A. fumigatus wild-type strain (strain CM237). Only transformants which had incorporated the tandem repeat in the promoter of the cyp51A gene and the L98H amino acid substitution exhibited similarly reduced patterns of susceptibility to all triazole agents and similarly increased levels of cyp51A expression, confirming that the combination of both alterations was responsible for the azole-resistant phenotype.
The influence of women on the health care-seeking behavior of men.

BACKGROUND Gender and social relationships are believed to have a strong influence on health care attitudes and behavior. This study was designed to determine the effect of the gender of closely associated persons on the health care-seeking behavior of persons of the opposite sex. METHODS We developed a 14-item questionnaire that requested information on social and demographic characteristics, health status, and influences on the decision to seek health care, and administered it to 314 consecutive patients seen at two family medicine clinics in San Diego, California. Data were analyzed by means of one way analysis of variance for continuous variables and the chi-square test for categorical variables. Additionally, data were analyzed by means of a multivariant logistic regression model that calculated odds ratios and 95% confidence intervals. RESULTS Men were 2.7 times more likely than women to be influenced to seek health care by a member of the opposite sex (95% CI, 1.6 to 4.6). Married patients were 2.4 times more likely than unmarried patients to be influenced to seek health care by a member of the opposite sex (95% CI, 1.4 to 4.3). CONCLUSIONS Women exert an important influence on the decisions of men to seek health care.
Relationships between Meaningful Activity, Basic Psychological Needs, and Meaning in Life: Test of the Meaningful Activity and Life Meaning Model

The author of this study proposed and examined a theoretical model in which meaningful activity fulfills basic psychological needs and contributes to meaning in life. Hypotheses derived from the Meaningful Activity and Life Meaning model and tested within this study included: (1) meaningful activity will be associated with meaning in life, (2) meaningful activity will be associated with basic psychological needs, and (3) basic psychological needs will partially mediate the relationship between meaningful activity and meaning in life. Structural equation modeling with latent variables was used to test the study hypotheses. A total of 591 undergraduate and graduate students completed the Engagement in Meaningful Activities Survey, the Basic Psychological Needs Scales (i.e., autonomy, competence, and relatedness), and the Presence subscale of the Meaning in Life Questionnaire. Results from the study were in full support of each hypothesis and indicate the important role meaningful activity may serve in fostering meaning in life.
Differentiation of Pancreatic Tumours by Conventional Ultrasound, Unenhanced and Echo-Enhanced Power Doppler Sonography

Background: Echo-enhanced power Doppler sonography (power Doppler sonography after injection of a contrast agent) is a non-invasive and increasingly used procedure for differentiating between pancreatic tumours. However, the diagnostic accuracy of this method compared to conventional ultrasound or unenhanced power Doppler sonography has never been investigated in a large prospective controlled study. Methods: 137 patients were included in the study, selected from 190 consecutive patients with a mean age of 60 years (range 16-85) who presented at our department in the period January 1998 through June 2001 with clinical suspicion of a pancreatic tumour. Sonography was performed by an experienced examiner blind to the patients' clinical diagnoses. The exact diagnosis was based on histological evidence from biopsy examination (surgical and fine needle biopsy) or on a follow-up of at least 18 months. Results: Of the 137 patients, 47 had pancreatic cancer; 41 had masses associated with pancreatitis; 17 had neuroendocrine tumours; 12 had cystic lesions of the pancreas; and 10 had other pancreatic diseases. A normal pancreas was found in 10 patients. The sensitivity of echo-enhanced power Doppler sonography with respect to diagnosing pancreatic carcinoma was 87% and its specificity 94%. The corresponding values for chronic pancreatitis were 85% and 99%, respectively. Conclusions: Echo-enhanced power Doppler sonography has a high sensitivity and specificity in the differential diagnosis of pancreatic tumours. However, histology is the standard of reference.
Quality of care from the perspective of elderly people: the QUOTE-elderly instrument.

BACKGROUND patient views on the quality of care are usually assessed by means of patient satisfaction questionnaires. AIM to develop an instrument that would: (i) produce data related to the expectations and experiences of noninstitutionalized elderly people, (ii) contain items that had been formulated in collaboration with elderly people, (iii) measure quality from the perspective of the users of health care services and (iv) produce data on generic quality aspects and quality aspects specifically related to the needs of elderly people. METHODS we developed the instrument for measuring quality of care from the perspective of non-institutionalized elderly people (QUOTE-Elderly) by using a combination of qualitative and quantitative methods. We obtained empirical data on the opinions and experiences of 338 elderly people. We evaluated the taxonomy of the instrument, internal consistency of (sub)scales and the feasibility of the instrument using explorative and confirmative factor analyses and reliability analysis. RESULTS using scale optimization, we produced a self-administered questionnaire on quality of health care from the perspective of elderly people. This contains scientific characteristics and provides specific information for practical quality-assurance policies.
Radial glia defines boundaries and subdivisions in the embryonic midbrain of the lizard Gallotia galloti

We have studied the organization of the midbrain radial glia in embryos of Gallotia galloti using the fluorescent lipophilic dye 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine perchlorate (DiI) and the antibodies H5 and RC2. Our goal was to verify if the radial glia takes part in the midbrain boundaries formation and if it defines different zones. Our exam reveals two clear limits, anterior or mesencephalic–diencephalic (m/d) and posterior or mesencephalic–rhombencephalic (m/r), that can be defined as the borders where the midbrain radial glia processes end. Moreover, fasciculate radial glia processes characterize these limits totally or partially. They coincide with gene expression limits and with cytoarchitectonic limits defined by other criteria. Six different subdivisions, five alar and one basal, can be defined according to radial glia distribution, fasciculation, and immunohistochemical features. The ventral part of the alar region is defined by an RC2‐positive bundle of radial glial cells. This bundle supposes a trustworthy landmark to point out the tectal/tegmental boundary. We hypothesize that this pattern of midbrain radial glia represents a basic model in amniota. J. Comp. Neurol. 473:162–176, 2004. © 2004 Wiley‐Liss, Inc.
Effects of raw and heat-treated bambara groundnut (vigna subterranea) on the performance and body composition of growing broiler chicks

Bambara groundnut (Vigna subterannea) – a leguminous root nut, which grows in the tropics and subtropics – was used in an experiment to test its effect on the performance of growing broiler chicks. Performance was measured by monitoring growth, measuring nutrients and energy balances and also by determining nutrient accretion. Twelve-day old broiler chicks, kept in individual metabolic cages, were used in an experiment in which birds were equally allotted (n = 10) into a control (fed high performance diet made up principally of wheat and soybean meal) and 6 test groups fed diets containing 19, 76 and 95% raw or autoclaved bambara groundnuts. All diets were similar in energy, nitrogen and total lipid contents. Diets were also balanced for amino acids, vitamins and minerals in accordance to the specific requirements of the birds. Feed and water were provided ad libitum. Growth performance was monitored over 15 days. Nutrient and energy balances were measured by use of TiO2 as marker. The comparative slaughter technique was implored for the determination of energy and nutrient accretion. The results showed that the level of raw or autoclaved bambara had no significant influence on feed intake. Increasing the bambara level in the diets caused a general linear drop in the performance of the broilers. However, with up to 95% bambara in the diet, the general performance was still above 75% compared to the control. Increasing levels of bambara groundnut caused a decrease in the metabolizability of energy and efficiency of utilisation of ME for BWG. The animals retained more water with increasing levels of raw bambara in the diets while autoclaving increased their DM content to levels comparable to the control. The protein composition of gain was comparable at all levels of inclusion. In general autoclaving improved the performance of bambara.