Miracle-dz/UniFAR-FTUs huggingface dataset:
Report of a fourth individual with a lethal syndrome of choanal atresia, athelia, evidence of renal tubulopathy, and family history of neck cysts

In 1998 , Hisama et al. described three brothers born following pregnancies complicated by oligohydramnios in the second or third trimester. Post‐natal renal functions were compromised, and post‐mortem studies showed kidney tissue abnormalities. Resuscitation and support were required, and they survived 25 hr to 12 days. All boys had a similar craniofacial appearance with infraorbital creases, low set dysplastic ears, as well as athelia, and broad digits with small nails. Variably present features were choanal atresia, preauricular tags and pits, branchial clefts, ventricular septal defect, type IIB interrupted aortic arch, pulmonary lobation anomaly, absent gallbladder, absent thymus, absent parathyroid gland, accessory spleen, and imperforate anus. Their mother, maternal grandmother, and maternal first cousin had neck cysts removed as children. We describe a female infant born following a pregnancy where oligohydramnios developed between 27 and 33 weeks gestation. Renal function was initially impaired, but improved over the first weeks of life. There was however a persistent renal wasting of magnesium. Her craniofacial appearance with infraorbital creases and low set dysplastic ears was similar to the brothers' described by Hisama et al. [1998; Am J Med Genet 80:335–342]. She had choanal atresia, athelia, a preauricular pit, gingival cysts, broad digits with small nails, right aortic arch with a vascular ring, hypothyroidism, impaired glucose homeostasis, hypoadrenalism, neurological impairment, and brain calcifications on CT. She died after 13 weeks of intensive care. Her mother as a child and maternal grandfather as an adult had neck cysts removed. The similarities between the cases suggest a common syndrome. © 2007 Wiley‐Liss, Inc.
Chronic Nitric Oxide Synthase Inhibition Prevents New Coronary Capillary Generation

l-NAME-induced hypertension has been shown to produce concentric (eutrophic) remodeling of the heart despite an enhanced afterload. We postulated that nitric oxide synthase inhibition could limit coronary capillary growth to explain the nature of remodeling. To test our hypothesis, we aimed at determining the effect of endogenous and exogenous nitric oxide on coronary neovascularization. Aortic and coronary rings from normotensive animals were incubated in a three-dimensional type I collagen matrix in the presence of l-NAME or the nitric oxide donor SNAP. l-NAME inhibited, while SNAP stimulated, neovascularization from aortic and coronary rings after 12 days of in vitro incubation. In arterial rings harvested from rats treated with l-NAME for 14 days and in which no further in vitro treatment was added, only coronary rings showed a reduction in new capillary generation. While confirming that chronic l-NAME-treated rats develop concentric remodeling, the evaluation of capillary density did not reveal any difference as compared with the controls in 3 areas of the myocardium. In conclusion, chronic inhibition of nitric oxide synthesis in vivo produces a long-lasting reduction in the capacity of coronary arteries to generate new capillaries in vitro. Thus, our results lend support to the hypothesis that an inhibition of new capillary formation could prevent the development of compensatory ventricular hypertrophy, in favor of concentric remodeling.
Oxidative‐stress and IL‐6 mediate the fibrogenic effects of rodent Kupffer cells on stellate cells

The impact of Kupffer cells (KCs) on the hepatic stellate cell (HSC) fibrogenic response was examined in an in vitro coculture model of primary KCs and HSCs. Coculture with KCs induced a more activated phenotype and greater proliferation compared to HSC cultured alone. Similar results were obtained on Matrigel which maintains HSCs quiescent. The effect of KCs on HSC collagen I involved transcriptional regulation, as determined by nuclear in vitro transcription run‐on assays, promoter studies, and Northern blot analysis, while stability of the COL1A1 and COL1A2 mRNA were similar. The minimal COL1A1 and COL1A2 promoter regions responsible for the KC effects were localized to the −515 and −378 base pair (bp) regions, respectively. Intracellular and extracellular collagen I protein, H2O2, and IL‐6 increased in a time‐dependent fashion, especially for HSCs in coculture. Catalase prevented these effects as well as the transactivation of both collagen promoters. The rate of collagen I protein synthesis and intracellular collagen I degradation remained similar but the t1/2 of the secreted collagen I was lower for HSC in coculture. MMP13, a protease that degrades extracellular collagen I, decreased in the cocultures, while TIMP1, a MMP13 inhibitor, increased; and these effects were prevented by catalase, anti‐IL‐6, and siRNA‐IL‐6. Cocultured HSC showed elevated phosphorylation of p38 which when inhibited by catalase, anti‐IL‐6, and siRNA‐IL‐6 it blocked TIMP1 upregulation and collagen I accumulation. In conclusion, these results unveil a novel dual mechanism mediated by H2O2 and IL‐6 by which KCs may modulate the fibrogenic response in HSCs. (HEPATOLOGY 2006;44:1487–1501.)
The combination of oral amphotericin B with azoles prevents the emergence of resistant Candida species in neutropenic patients

The role of antifungal prophylaxis remains controversial and concerns exist that the use of azoles may potentiate the emergence of resistant Candida species. We used a strategy of combining the latest azole/triazole with oral amphotericin B to reduce this risk. We analysed data on Candida colonization and candidaemia in neutropenic patients from four prophylaxis periods (1985/6: ketoconazole and amphotericin B suspension; 1991/2 & 1997: fluconazole and amphotericin B suspension; 1998/9: itraconazole) to look for evidence of the emergence of potentially resistant species. Overall, the percentage of patients colonized with Candida fell significantly (69·3%, 57·5%, 43·2% and 46%, respectively, P < 0·001) due to a decrease in colonization with C. albicans (49%, 23·1%, 22·2% and 25·2%, respectively, P < 0·001). However, in 1998/9, increased colonization, particularly with C. glabrata in the lower gastrointestinal tract, was noted to coincide with the omission of oral amphotericin B. Despite an increasing population of ‘high risk’ patients, the incidence of candidaemia has not changed significantly (2%, 1·4%, 1·2% and 2% respectively). However, species causing candidaemia have changed, with resistant organisms now predominating. Our findings support the use of azole prophylaxis although, in view of the trends noted when itraconazole was used alone, we would recommend the additional use of oral amphotericin B.
Aloe vera extract activity on human corneal cells

Context: Ocular diseases are currently an important problem in modern societies. Patients suffer from various ophthalmologic ailments namely, conjunctivitis, dry eye, dacryocystitis or degenerative diseases. Therefore, there is a need to introduce new treatment methods, including medicinal plants usage. Aloe vera [Aloe barbadensis Miller (Liliaceae)] possesses wound-healing properties and shows immunomodulatory, anti-inflammatory or antioxidant activities. Materials and methods: NR uptake, MTT, DPPH• reduction, Griess reaction, ELISA and rhodamine-phalloidin staining were used to test toxicity, antiproliferative activity, reactive oxygen species (ROS) reduction, nitric oxide (NO) and cytokine level, and distribution of F-actin in cells, respectively. Aim: The present study analyzes the effect of Aloe vera extracts obtained with different solvents on in vitro culture of human 10.014 pRSV-T corneal cells. Results: We found no toxicity of ethanol, ethyl acetate and heptane extracts of Aloe vera on human corneal cells. No ROS reducing activity by heptane extract and trace action by ethanol (only at high concentration 125 µg/ml) extract of Aloe vera was observed. Only ethyl acetate extract expressed distinct free radical scavenging effect. Plant extracts decreased NO production by human corneal cells as compared to untreated controls. The cytokine (IL-1β, IL-6, TNF-α and IL-10) production decreased after the addition of Aloe vera extracts to the culture media. Discussion and conclusions: Aloe vera contains multiple pharmacologically active substances which are capable of modulating cellular phenotypes and functions. Aloe vera ethanol and ethyl acetate extracts may be used in eye drops to treat inflammations and other ailments of external parts of the eye such as the cornea.
ABORIGINAL ORAL TRADITIONS OF AUSTRALIAN IMPACT CRATERS Duane

In this paper we explore Aboriginal oral traditions that relate to Australian meteorite craters. Using the literature, first-hand ethnographic records and fieldtrip data, we identify oral traditions and artworks associated with four impact sites: Gosses Bluff, Henbury, Liverpool and Wolfe Creek. Oral traditions describe impact origins for Gosses Bluff, Henbury and Wolfe Creek Craters, and non-impact origins for Liverpool Crater, with Henbury and Wolfe Creek stories having both impact and non-impact origins. Three impact sites that are believed to have been formed during human habitation of Australia—Dalgaranga, Veevers, and Boxhole—do not have associated oral traditions that are reported in the literature.
This is what I need a clinical feedback system to do for me: A qualitative inquiry into therapists’ and patients’ perspectives

Abstract Routine outcome monitoring and clinical feedback systems (ROM/CFSs) are promising methods of providing naturalistic research data and enhancing mental health care. However, implementation in routine care is challenging, and we need more knowledge about clinicians’ and patients’ needs from such systems. Objective: We aimed to study perspectives of clinicians and patients to explore how ROM/CFS can be helpful and acceptable to them. Method: We interviewed 55 participants in focus groups and individual interviews and analyzed the data through rigorous team-based qualitative analyses. Results: We report 3 overarching domains: (a) Shared needs, (b) Specific patient needs, and (c) Specific therapist needs. Shared needs, in which perspectives of different stakeholders converge, was the dominant domain in the material. Under each domain, we report 3 specific themes: (a1) Degree of trust in therapy, (a2) Allowing for openness, (a3) Monitoring joint objectives; (b1) Life functioning, (b2) Canary in the coal mine, (b3) Holistic report; and (c1) Emotional presence and style, (c2) Monitoring risk and symptoms, and (c3) Agency and ownership of process. Conclusions: In what should increase our confidence toward core aspects of ROM, we suggest that an integration of relational feedback concepts and stringent clinical dimension tracking into the ROM/CFS can be beneficial.
Transdermal immunization with large proteins by means of ultradeformable drug carriers

By means of novel, ultradeformable and self‐optimizing agent carriers called transfersomes, large molecules can be brought into the body through intact permeability barriers. This permits non‐invasive immunization through normal skin and gives rise to a similar or even slightly higher antibody titer than subcutaneous injections of the same immunogen formulation. The former type of immunization also results in a higher IgA/IgG ratio in the blood than the repeated immunogen injections, as shown here for a soluble protein, human serum albumin, as well as for an integral membrane protein, gap junction protein, in mice.
Numerical Terradynamic Simulation Group 12-2014 Asynchronous Amazon forest canopy phenology indicates adaptation to both water and light availability

Amazon forests represent nearly half of all tropical vegetation biomass and, through photosynthesis and respiration, annually process more than twice the amount of estimated carbon (CO2) from fossil fuel emissions. Yet the seasonality of Amazon canopy cover, and the extent to which seasonal fluctuations in water availability and photosynthetically available radiation influence these processes, is still poorly understood. Implementing six remotely sensed data sets spanning nine years (2003–2011), with reported field and flux tower data, we show that southern equatorial Amazon forests exhibit a distinctive seasonal signal. Seasonal timing of water availability, canopy biomass growth and net leaf flush are asynchronous in regions with short dry seasons and become more synchronous across a west-to-east longitudinal moisture gradient of increasing dry season. Forest cover is responsive to seasonal disparities in both water and solar radiation availability, temporally adjusting net leaf flush to maximize use of these generally abundant resources, while reducing drought susceptibility. An accurate characterization of this asynchronous behavior allows for improved understanding of canopy phenology across contiguous tropical forests and their sensitivity to climate variability and drought. S Online supplementary data available from stacks.iop. org/ERL/9/124021/mmedia
Efficiency of Drag Sampling for Estimating Population Sizes ofIxodes pacificus (Acari: Ixodidae) Nymphs in Leaf Litter

Abstract Drag sampling is a commonly used method to obtain relative estimates of the density of questing nymphalIxodes scapularis Say andI. pacificus Cooley & Kohls ticks, which are primary vectors of Lyme disease spirochetes to humans in North America. However, the efficiency of drag sampling in determining absolute population densities of questing nymphs has not been evaluated previously. Therefore, we assessed the efficiency of a single drag-sampling occasion to estimate the total population size of questingI. pacificus nymphs in a leaf-litter habitat in California. Repeated daily removal sampling was carried out in four areas, each covering 300 m2, on 17 occasions over a 23-d period in the spring of 1999. In total, 573I. pacificus nymphs were collected, of which 55 (9.6%) were collected on the initial sampling occasion and 20 (3.5%) on the last occasion. The total population size of questing nymphs, i.e., the intersection with the horizontal axis of a linear regression of daily nymphal catch rates on the number of nymphs caught previously, was estimated to be 936. Thus, the efficiency of the initial sampling occasion to estimate the total population size was 5.9% (4.8, 5.0, 5.8, and 9.1%, respectively, for the four individual sampling areas). Further, the overall mean efficiencies of the two, five, and 10 first removal sampling occasions to estimate the absolute nymphal density was 5.2, 4.7, and 4.3%, respectively, and 13 sampling occasions were required to collect 50% of the estimated total nymphal population.
Paradoxical Hypotension during Dobutamine Infusion for Myocardial Perfusion Scintigraphy

Summary Aim: Dobutamine as a predominant beta-1 agonist increases heart rate and myocardial contractility and at sufficient high doses, it also increases systolic blood pressure. This study was undertaken to describe instances of paradoxical hypotension during dobutamine infusion for TI-201 myocardial perfusion SPECT study and the relationship between scintigraphic findings and hypotension occurred during dobutamine infusion. Methods: In 201 consecutive patients unable to perform adequate exercise, dobutamine TI-201 myocardial SPECT was performed. Dobutamine was infused starting from 10 μg/kg/min increasing to 40 μg/kg/min. Paradoxical hypotension was defined as a decrease in systolic blood pressure ≥ 20 mmHg compared with baseline study. Results: Paradoxical hypotension was observed in 40 patients (Group A) out of 201 (19.9%) while no significant change in systolic blood pressure was detected in the remaining 161 patients (Group B). Mean maximum fall in systolic blood pressure was 39 ± 18 mmHg (range: 20-90). In 33 of 40 patients (83%) with paradoxical hypotension, scintigraphy was normal compared to 131 (81%) of the remaining 161 patients. In patients of Group A, angiography, echocardiography and tilt table tests were performed in 13, 11 and 6 patients respectively. Nine of 13 angiographic evaluations (69%), 10 of 11 echocardiographic evaluations (91%), all of the tilt table tests were normal. Additionally, all of the patients of Group A were clinically followed up at least 6 months after the myocardial perfusion scintigraphy. None of the patients had a cardiac event except one patient during the follow-up period. Conclusion: Paradoxical hypotension during dobutamine infusion for myocardial scintigraphy is not an uncommon finding and up to 19.9% patients may develop such hypotension. To maximize test safety, precautions should be taken during dobutamine myocardial stress test, since remarkable decrease in systolic blood pressure may occur. Unlike hypotension occurring with exercise test, hypotension response to dobutamine is not always a marker for coronary artery disease.
Comparison of the Home Advantage in Nine Different Professional Team Sports in Spain

Home advantage is a well-established phenomenon in many sports. The present study is unique in that it includes different sports analysed in the same country, at the same level of competition, and over the same time period. Nine team sports from Spain were included: baseball, basketball, handball, indoor soccer, roller hockey, rugby, soccer, volleyball, and water polo. Data for five seasons (2005–2006 to 2009–2010) were obtained, totaling 9,472 games. The results confirmed the existence of home advantage in all nine sports. There was a statistically significant difference between the sports; home advantage was highest in rugby (67.0%), and lowest in volleyball (55.7%), water polo (56.2%), and roller hockey (58.3%). The design of the study controlled for some of the likely causes of home advantage, and the results suggested that the high home advantage for rugby was likely a reflection of the continuous, aggressive, and intense nature of the sport.
Peptidoglycan structure of Lactobacillus casei, a species highly resistant to glycopeptide antibiotics

The structure of the peptidoglycan of Lactobacillus casei ATCC 393, a species highly resistant to glycopeptide antibiotics, was examined. After digestion, 23 muropeptides were identified; monomers represented 44.7% of all muropeptides, with monomer tetrapeptides being the major ones. Fifty-nine percent of the peptidoglycan was O-acetylated. The cross-bridge between D-alanine and L-lysine consisted of one asparagine, although aspartate could be found in minor quantities. Since UDP-MurNAc-tetrapeptide-D-lactate is the normal cytoplasmic precursor found in this species, monomer tetrapeptide-lactate was expected to be found. However, such a monomer was found only after exposure to penicillin, suggesting that penicillin-sensitive D,D-carboxypeptidases were very active in normal growing cells.
Gape size, its morphological basis, and the validity of gape indices in western diamond‐backed rattlesnakes (crotalus atrox)

Maximum gape is important to the ecology and evolution of many vertebrates, particularly gape‐limited predators, because it can restrict the sizes and shapes of prey that can be eaten. Although many cranial elements probably contribute to gape, it is typically estimated from jaw length or jaw width, or occasionally from a combination of these two measures. We measured maximum gape directly for 18 individuals of the western diamond‐backed rattlesnake, Crotalus atrox. We measured each individual's body length, several external cranial dimensions, several cranial osteological dimensions from cleaned skeletons, and we calculated gape index values from two published gape indices (GI). Cranial bone lengths and gape circumference showed negative allometry with snout–vent length (SVL), indicating that small individuals have relatively larger heads and gapes than their larger conspecifics. We then used Akaike's Information Criterion to determine which external and osteological measurements were the best predictors of gape. Body size (SVL) was the best predictor of maximum gape overall; however, when SVL was excluded from the analysis, quadrate (QL) and mandible lengths (MdLs) were the best predictors of maximum gape using both external and osteological measurements. Quadrate length probably contributes directly to gape; however, the importance of MdL to gape is less clear and may be due largely to its allometric relationships with head length and SVL. The two published GI did not prove to be better indicators of actual gape than the jaw and QLs in this study, and the gape values they produced differed significantly from our empirically determined gapes. For these reasons, we urge caution with the use and interpretation of computed GI in future studies. The extensive variation in quadrate and mandible morphology among lineages suggest that these bones are more important to variation in gape among species and lineages than within a single species. J. Morphol. , 2013. © 2012 Wiley Periodicals, Inc.
Ageing and obesity similarly impair antibody responses

Ageing is characterized by increased low‐grade chronic inflammation, which is a significant risk factor for morbidity and mortality of elderly individuals. Similar to ageing, obesity is considered to be an inflammatory predisposition associated with chronic activation of immune cells and consequent local and systemic inflammation. Both ageing and obesity are characterized by reduced innate and adaptive immune responses. This review focuses on B cells, how they may contribute, at least locally, to low‐grade chronic inflammation in ageing and obesity and on the mechanisms involved.
Cold denaturation-induced conformational changes in phosphoglycerate kinase from yeast.

The temperature-dependent conformational equilibrium of 3-phosphoglycerate kinase has been studied in the temperature range from 1 to 30 degrees C by means of dynamic light scattering, small-angle X-ray scattering, differential scanning calorimetry, circular dichroism spectroscopy, and fluorescence spectroscopy. At 28 degrees C and in the presence of 0.7 M guanidine hydrochloride (GuHCl), the radius of gyration (RG) and the Stokes radius (RS) are 2.44 and 3.09 nm, respectively. Decreasing the temperature effects unfolding of the molecule, a process that involves two stages. The two stages correspond to the successive unfolding of the N-terminal and C-terminal domains. The peak maxima of the excess heat capacity, determined from differential calorimetric scans, extrapolated to 0 scan rate, are positioned at 16.5 degrees C for the N-terminal domain and at 6.3 degrees C for the C-terminal domain. At 4.5 degrees C, the radius of gyration and the Stokes radius increase to 7.8 and 4.8 nm, respectively. The persistence length and the length of the statistical chain segment of the unfolded polypeptide chain are 1.74 and 3.48 nm, corresponding to five and ten amino acids, respectively. At 1 degrees C, the dimensions of the unfolded chain nearly agree with the predicted dimensions under theta conditions. Thus, the conformational changes upon cold denaturation can be described by a transition from a compactly folded molecule to a random coil. The conformation-dependent ratio rho = RGRS-1 increases from rho = 0.79 to rho = 1.63. The volume of the unfolded chain is 30 times larger than that of the folded chain in the native state. (ABSTRACT TRUNCATED AT 250 WORDS)