Miracle-dz/UniFAR-FTUs huggingface dataset:
Student Use of Self-Data for Out-of-Class Graphing Activities Increases Student Engagement and Learning Outcomes†

Two out-of-class graphing activities related to hormonal regulation of the reproductive cycle and stress responses are used to determine whether student use of self-data vs. provided data increases engagement, learning outcomes, and attitude changes. Comparisons of quizzes and surveys for students using self- vs. provided data suggest that while both activities increase learning outcomes, use of self-data compared with provided data has a greater impact on increasing learning outcomes, promotes recognition that hormones are relevant, and enhances confidence in graphing skills and graphing efficacy.
The Fountain of Health: effective health promotion knowledge transfer in individual primary care and group community-based formats

ABSTRACT Background: The Fountain of Health (FoH) initiative is a knowledge transfer (KT) project on the science of brain health and resilience promotion, in alignment with positive psychiatry. Objectives: Assess the effectiveness of FoH KT delivered in individual and group-based formats. Design: Pre- and post-intervention quality assurance survey of FoH KT. Setting: Interventions occurred in Nova Scotia, Canada. Participants: Adults over age 50 years without pre-existing dementia were targeted. A total of 92 participants received FoH KT in individualized (n = 41) and group-based (n = 51) formats. Intervention: FoH KT (e.g. sharing evidence, lifestyle coaching, and goal setting) using a range of KT supports (e.g. FoH website, paper materials) was delivered to (1) individual patients by primary care clinicians and (2) community-based groups by lay leaders. Measurements: The main outcome measure was participant pre- and post-quality assurance self-reports. Results: Improvements were found in participant awareness of FoH, knowledge of evidence-based mental health promotion initiatives, and in application of this information in daily life in both individual and group-based settings. Improvements in participant knowledge about epigenetic factors that impact health and confidence with health behavior goal setting were reported in both contexts. Changes in self-perceptions of aging scores reached significance in the group intervention. Conclusions: FoH KT produced short-term positive self-reported changes in participants in both individual and group formats. Larger control studies with long-term follow up are needed to better assess effects of both individual and group formats of FoH KT and longer term impacts on health behaviors and outcomes.
Fortune Favors the Brave: Tactical Behaviors in the Middle-Distance Running Events at the 2017 IAAF World Championships.

PURPOSE To assess tactical and performance factors associated with progression from qualification rounds in the 800-m and 1500-m running events at the 2017 International Association of Athletics Federations World Championships. METHODS Official results were used to access final and intermediate positions and times, as well as performance characteristics of competitors. Shared variance between intermediate positions and rank order lap times with finishing positions were calculated, along with probability of automatic qualification, for athletes in each available race position at the end of every 400-m lap. Differences in race positions and lap times relative to season's best performances were assessed between automatic qualifiers, fastest losers, and nonqualifiers. RESULTS Race positions at the end of each 400-m lap remained more stable through 800-m races than 1500-m races. Probability of automatic qualification decreased with both race position and rank order lap times on each lap, although rank order lap times accounted for a higher degree of shared variance than did intermediate position. In the 1500-m event, fastest losers ran at a higher percentage of season's best speed and adopted positions closer to the race lead in the early stages. This was not the case in the 800-m. CONCLUSIONS Intermediate positioning and the ability to produce a fast final race segment are strongly related to advancement from qualification rounds in middle-distance running events. The adoption of a more "risky" strategy characterized by higher speeds relative to season's best may be associated with an increased likelihood of qualification as fastest losers in the 1500-m event.
High plasma levels of ghrelin and des-acyl ghrelin in responders to antiepileptic drugs

Objective To reconsider ghrelin and des-acyl ghrelin plasma levels in children with epilepsy in order to establish a possible relation with response to antiepileptic drugs (AEDs). Methods We designed an observational study in which 114 patients with epilepsy were classified as responders (77) or nonresponders (37) and compared to 59 controls. In these patients, we measured ghrelin and des-acyl ghrelin by immunoassays in blood samples obtained after overnight fast. Results Ghrelin plasma levels were higher (+94%; p < 0.001, Dunn test) in responders compared to controls. Des-acyl ghrelin plasma levels were also higher in the same group (+55%; p < 0.001). In addition, both hormones were unmodified in nonresponders compared to controls. By comparing responders to nonresponders, ghrelin and des-acyl ghrelin, respectively, were +126% (p < 0.001) and +29% (p < 0.001) in patients with a positive response to AEDs. Conclusions These results indicate that ghrelin and des-acyl ghrelin plasma levels are especially high in patients with epilepsy who positively respond to AEDs. In view of the anticonvulsant properties of ghrelin and des-acyl ghrelin, we propose that their higher levels could play a role in modulating the response to AEDs. Moreover, these peptides could be promising markers of response to AEDs.
Recent Advances and Ongoing Challenges in the Diagnosis of Microbial Infections by MALDI-TOF Mass Spectrometry

Timeliness and accuracy in the diagnosis of microbial infections are associated with decreased mortality and reduced length of hospitalization, especially for severe, life-threatening infections. A rapid diagnosis also allows for early streamlining of empirical antimicrobial therapies, thus contributing to limit the emergence and spread of antimicrobial resistance. The introduction of matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) for routine identification of microbial pathogens has profoundly influenced microbiological diagnostics, and is progressively replacing biochemical identification methods. Compared to currently used identification methods, MALDI-TOF MS has the advantage of identifying bacteria and yeasts directly from colonies grown on culture plates for primary isolation in a few minutes and with considerable material and labor savings. The reliability and accuracy of MALDI-TOF MS in identification of clinically relevant bacteria and yeasts has been demonstrated by several studies showing that the performance of MALDI-TOF MS is comparable or superior to phenotypic methods currently in use in clinical microbiology laboratories, and can be further improved by database updates and analysis software upgrades. Besides microbial identification from isolated colonies, new perspectives are being explored for MALDI-TOF MS, such as identification of pathogens directly from positive blood cultures, sub-species typing, and detection of drug resistance determinants. In this review, we summarize the state of the art in routine identification of microbial pathogens by MALDI-TOF MS, and highlight recent advancements of this technology in special applications, such as strain typing, assessment of drug susceptibility, and detection of virulence factors.
Care Transitions From Patient and Caregiver Perspectives

PURPOSE Despite concerted actions to streamline care transitions, the journey from hospital to home remains hazardous for patients and caregivers. Remarkably little is known about the patient and caregiver experience during care transitions, the services they need, or the outcomes they value. The aims of this study were to (1) describe patient and caregiver experiences during care transitions and (2) characterize patient and caregiver desired outcomes of care transitions and the health services associated with them. METHODS We interviewed 138 patients and 110 family caregivers recruited from 6 health networks across the United States. We conducted 34 homogenous focus groups (103 patients, 65 caregivers) and 80 key informant interviews (35 patients, 45 caregivers). Audio recordings were transcribed and analyzed using principles of grounded theory to identify themes and the relationship between them. RESULTS Patients and caregivers identified 3 desired outcomes of care transition services: (1) to feel cared for and cared about by medical providers, (2) to have unambiguous accountability from the health care system, and (3) to feel prepared and capable of implementing care plans. Five care transition services or provider behaviors were linked to achieving these outcomes: (1) using empathic language and gestures, (2) anticipating the patient’s needs to support self-care at home, (3) collaborative discharge planning, (4) providing actionable information, and (5) providing uninterrupted care with minimal handoffs. CONCLUSIONS Clear accountability, care continuity, and caring attitudes across the care continuum are important outcomes for patients and caregivers. When these outcomes are achieved, care is perceived as excellent and trustworthy. Otherwise, the care transition is experienced as transactional and unsafe, and leaves patients and caregivers feeling abandoned by the health care system.
HIV-1 integrase strand-transfer inhibitor resistance in southern Taiwan

The use of antiretroviral therapy has reduced rates of mortality and morbidity in patients with human immunodeficiency virus/acquired immune deficiency syndrome(HIV/AIDS). However, transmission of drug-resistant strains poses a challenge to control the spread of HIV-1. Primary resistance to integrase strand-transfer inhibitors (INSTIs) is rare despite their increased use. The prevalence of transmitted drug resistance (TDR) to INSTIs was 0.9% in northern Taiwan. This study was to analyse the prevalence and risk factors of TDR to INSTIs in southern Taiwan. In this study, we enrolled antiretroviral treatment-naïve HIV-1-infected subjects who underwent voluntary counselling and testing from 2013 to 2016 in southern Taiwan. Genotypic drug resistance, coreceptor tropism (CRT) and INSTI resistance were determined. Logistic regression was used to analyse the risk factors for INSTI polymorphic substitution. Sequences were obtained from 184 consecutive individuals, of whom 96.7% were men who have sex with men and 3.3% were heterosexual. Of the patients, 10% (19/183) had hepatitis B and 33.3% (61/183) had syphilis infection. Subtype B HIV-1 strains were found in 96.1% of the patients. Fifteen patients (8.4%, 15/178) harboured nucleoside reverse transcriptase inhibitors, non-nucleoside reverse transcriptase inhibitors or protease inhibitors resistance. CCR-5 coreceptors were used by 71.4% (130/184) of the patients. None of the patients had INSTI resistance-associated mutations, however 16 patients had INSTI polymorphic substitutions, and they were associated with a higher HIV viral load (p = 0.03, OR 2.4, CI 1.1–5.3) and syphilis infection (p = 0.03, OR 3.7, CI 1.1–12.0). In conclusion, no signature INSTI resistance-associated mutations were detected in our cohort. Continued monitoring of TDR to INSTI is needed due to the increased use of INSTIs.
Deep Anomaly Detection Using Geometric Transformations

We consider the problem of anomaly detection in images, and present a new detection technique. Given a sample of images, all known to belong to a "normal" class (e.g., dogs), we show how to train a deep neural model that can detect out-of-distribution images (i.e., non-dog objects). The main idea behind our scheme is to train a multi-class model to discriminate between dozens of geometric transformations applied on all the given images. The auxiliary expertise learned by the model generates feature detectors that effectively identify, at test time, anomalous images based on the softmax activation statistics of the model when applied on transformed images. We present extensive experiments using the proposed detector, which indicate that our algorithm improves state-of-the-art methods by a wide margin.
Low Gain Servo Control During the Kohnstamm Phenomenon Reveals Dissociation Between Low-Level Control Mechanisms for Involuntary vs. Voluntary Arm Movements

The Kohnstamm phenomenon is a prolonged involuntary aftercontraction following a sustained voluntary isometric muscle contraction. The control principles of the Kohnstamm have been investigated using mechanical perturbations, but previous studies could not dissociate sensorimotor responses to perturbation from effects of gravity. We induced a horizontal, gravity-independent Kohnstamm movement around the shoulder joint, and applied resistive or assistive torques of 0.5 Nm after 20° angular displacement. A No perturbation control condition was included. Further, participants made velocity-matched voluntary movements, with or without similar perturbations, yielding a 2 × 3 factorial design. Resistive perturbations produced an increase in agonist electromyography (EMG), in both Kohnstamm and voluntary movements, while assistive perturbations produced a decrease. While overall Kohnstamm EMGs were greater than voluntary EMGs, the EMG responses to perturbation, when expressed as a percentage of unperturbed EMG activity, were significantly smaller during Kohnstamm movements than during voluntary movements. The results suggest that the Kohnstamm aftercontraction involves a central drive, coupled with low-gain servo control by a negative feedback loop between afferent input and a central motor command. The combination of strong efferent drive with low reflex gain may characterize involuntary control of postural muscles. Our results question traditional accounts involving purely reflexive mechanisms of postural maintenance. They also question existing high-gain, peripheral accounts of the Kohnstamm phenomenon, as well as accounts involving a central adaptation interacting with muscle receptors via a positive force feedback loop.
Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test

Aim This study was designed to evaluate the potential of the use of multiplex polymerase chain reaction (PCR) as an alternative to conventional antibiotic sensitivity test. Materials and Methods Isolates of Staphylococcus aureus (total = 36) from clinical cases presented to Teaching Veterinary Clinical Complex of College of Veterinary and Animal Sciences (CVAS), Navania, Udaipur, were characterized by morphological, cultural, and biochemical methods. Then, the isolates were further subjected to molecular characterization by PCR targeting S. aureus-specific sequence (107 bp). Phenotypic antibiotic sensitivity pattern was analyzed by Kirby-Bauer disc diffusion method against 11 commonly used antibiotics in veterinary medicine in and around Udaipur region. The genotypic antibiotic sensitivity pattern was studied against methicillin, aminoglycosides, and tetracycline targeting the gene mecA, aacA-aphD, and tetK by multiplex PCR. Results There was 100% correlation between the phenotype and genotype of aminoglycoside resistance, more than 90% correlation for methicillin resistance, and 58.3% in the case tetracycline resistance. Conclusion As there is a good correlation between phenotype and genotype of antibiotic resistance, multiplex PCR can be used as an alternative to the conventional antibiotic susceptibility testing, as it can give a rapid and true prediction of antibiotic sensitivity pattern.
The challenge of abandonment for the sustainable management of Palaearctic natural and semi-natural grasslands

Abstract Disturbance by biomass removal is a crucial mechanism maintaining the diversity of Palaearctic grasslands, which are unique biodiversity hotspots. The century-long traditional land use of mowing, grazing and burning, has been fundamentally changed in many parts of the Palaearctic. Due to socio-economic changes, large areas of former pastures and meadows have been abandoned, leading to a succession towards secondary scrublands or forest and the encroachment of competitor grass species, all leading to a decrease in biodiversity. Here we report the causes and consequences of the cessation of traditional grassland management regimes, provide strategies for reducing the impact of abandonment and consider these from the perspective of sustainability. We consider the possibilities for initiating sustainable management regimes in the contemporary socio-economic environment, and discuss the prospects and limitation of alternative management regimes in the conservation of grassland biodiversity. These themes are also the core topics of this Special Feature, edited by the EDGG. We hope that this Special Feature will encourage steps towards more sustainable strategies for the conservation of Palaearctic grasslands and the integration of the sustainability perspective into their conservation.
Interaction of Left Ventricular Size and Sex on Outcome of Cardiac Resynchronization Therapy Among Patients With a Narrow QRS Duration in the EchoCRT Trial

Background Longer QRS duration (QRSd) improves, but increased left ventricular (LV) end‐diastolic volume (LVEDV) reduces, efficacy of cardiac resynchronization therapy (CRT). QRSd/LVEDV ratios differ between sexes. We hypothesized that in the EchoCRT (Echocardiography Guided Cardiac Resynchronization Therapy) trial enrolling patients with heart failure with QRSd <130 ms, those with larger LVEDV would deteriorate but those with the highest QRSd/LVEDV would improve with CRT. Methods and Results Primary outcome in patients (n=787, 72% men, 93% New York Heart Association class III, QRSd <130 ms, LV ejection fraction ≤35%, LV dilation and dyssynchrony) randomized to CRT‐ON or CRT‐OFF and followed up for 19 months was compared according to LVEDV (height indexed) or QRSd/LVEDV ratio, in multivariable analysis. Structural remodeling was assessed echocardiographically 6 months after implantation. Patients with baseline LVEDV higher than or equal to median worsened with CRT (death/heart failure hospitalization: CRT‐ON versus CRT‐OFF, 35.2% versus 24.5% [hazard ratio, 1.64; 95% confidence interval, 1.11–2.42; P=0.012]), but those with LVEDV lower than median remained unaffected. Patients with the highest QRSd/LVEDV ratio improved with CRT (death/heart failure hospitalization in top quartile: 20.9% in CRT‐ON [n=91] versus 28.3% in CRT‐OFF [n=106] [hazard ratio, 0.64; 95% confidence interval, 0.34–1.24; P=0.188], versus the remaining quartiles: 31.7% in CRT‐ON [n=300] versus 24.8% in CRT‐OFF [n=290] [hazard ratio, 1.47; 95% confidence interval, 1.07–2.02; P=0.016], test for interaction P=0.046). QRSd and dyssynchrony were similar between groups. The 3‐way test for interaction indicated no sex‐specific effects. However, numerically, men with LVEDV higher than or equal to median accounted for worse outcomes of CRT‐ON. Women, with the highest QRSd/LVEDV ratio exhibited significant reverse remodeling. Conclusion CRT has opposite effects among patients with heart failure with QRSd <130 ms according to LV size: worsening outcomes in patients with larger LV, but inducing beneficial effects in those with smaller LV. Clinical Trial Registration URL: https://www.clinicaltrials. gov/. Unique identifier: NCT00683696.
Multifactorial Analysis of Mortality in Screening Detected Lung Cancer

We hypothesized that severity of coronary artery calcification (CAC), emphysema, muscle mass, and fat attenuation can help predict mortality in patients with lung cancer participating in the National Lung Screening Trial (NLST). Following regulatory approval from the Cancer Data Access System (CDAS), all patients diagnosed with lung cancer at the time of the screening study were identified. These subjects were classified into two groups: survivors and nonsurvivors at the conclusion of the NLST trial. These groups were matched based on their age, gender, body mass index (BMI), smoking history, lung cancer stage, and survival time. CAC, emphysema, muscle mass, and subcutaneous fat attenuation were quantified on baseline low-dose chest CT (LDCT) for all patients in both groups. Nonsurvivor group had significantly greater CAC, decreased muscle mass, and higher fat attenuation compared to the survivor group (p < 0.01). No significant difference in severity of emphysema was noted between the two groups (p > 0.1). We thus conclude that it is possible to create a quantitative prediction model for lung cancer mortality for subjects with lung cancer detected on screening low-dose CT (LDCT).
Occupational cholangiocarcinoma caused by exposure to 1,2‐dichloropropane and/or dichloromethane

A cluster of cholangiocarcinoma among printing company workers who were exposed to 1,2‐dichloropropane and/or dichloromethane was classified by the Ministry of Health, Labour and Welfare of Japan on 1 October 2013 as “occupational cholangiocarcinoma”. At the time of the diagnosis of cholangiocarcinoma, levels of γ‐glutamyl transferase, and aspartate and alanine aminotransferases were elevated, and had been elevated in some patients several years prior to the diagnosis. Regional dilatation of intrahepatic bile ducts without tumor‐induced obstruction was characteristic in diagnostic imaging. Pathological examination found chronic bile duct injury with DNA damage, precancerous/preinvasive lesions such as biliary intraepithelial neoplasia and intraductal papillary neoplasm of the bile duct in various sites of the large bile ducts, and invasive cholangiocarcinoma such as mass‐forming type and intraductal growth‐type intrahepatic cholangiocarcinoma and mainly papillary‐type extrahepatic cholangiocarcinoma. Whole‐exome analysis of the cancerous tissues showed hypermutation, substantial strand bias, and unique trinucleotide mutational changes. Patients seemed to suffer high incidence of postoperative complications including intra‐abdominal, which might be related to chronic bile duct injury. Postoperative recurrence from multicentric origins occurred in some patients, as DNA‐injured bile ducts have high carcinogenic potential. Aggressive treatment, including second resections for such multicentric recurrences, appeared to be effective. In 2014, the International Agency for Research on Cancer classified 1,2‐dichloropropane as Group 1 (carcinogenic to humans) and dichloromethane as Group 2A (probably carcinogenic to humans) carcinogens.
The mechanism of photocatalytic CO_2 reduction by graphene-supported Cu_2O probed by sacrificial electron donors

Cu_2O nanoparticles of 5 nm average size have been adsorbed (1.74 wt% loading) on defective graphene (Cu_2O/G) previously obtained by the pyrolysis of alginic acid sodium salt. The Cu_2O crystal phase was determined by XRD. XPS shows that the external layers of the Cu_2O nanoparticles are constituted mainly of Cu^+ although a certain percentage of Cu^II+ was also present. Cu_2O/G is a photocatalyst for the CO_2 reduction to methane in the presence of sacrificial agents, and the rate of CH_4 production depends on the oxidation potential of the electron donor. This relationship supports a mechanism involving photo-induced charge separation with the generation of electrons and holes. The highest CH_4 formation rate upon UV-Vis irradiation of Cu_2O/G with a 300 W Xe lamp was achieved for dimethylaniline reaching 326 µmol CH_4 per g per h. The spectral response of the Cu_2O photocatalyst shows, however, that the response of the photocatalyst is mainly due to UV irradiation, indicating that light absorption at the low Cu_2O loading on the Cu_2O/G photocatalyst occurs mainly on the graphene component.