Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Weissella cibaria CMU on Halitosis and Calculus, Plaque, and Gingivitis Indices in Beagles

Weissella (W.) cibaria strain Chonnam Medical University (CMU) has shown oral colonizing ability and inhibitory effects on the formation of volatile sulfur compounds (VSCs) in vitro studies. The present study was conducted to analyze the effects of the W. cibaria CMU on canine oral health. Halitosis, calculus, plaque, gingivitis, and intraoral microbiota were assessed in 3 groups: control (maltodextrin), W. cibaria CMU low concentration (CMU-L, 2 × 107 colony forming unit [CFU]), and high-concentration (CMU-H, 2 × 109 CFU). Halitosis was analyzed using both organoleptic evaluation and measurement of VSCs. Intraoral microbiota were analyzed by real-time polymerase chain reaction. From week 4, the total VSC level in the CMU-H group (4.0 ± 1.30 ng/10 mL) was significantly lower than in the control group (6.3 ± 2.28 ng/10 mL). Significant reduction in methyl mercaptan in the CMU-treated groups was also observed. In addition, the plaque index in the CMU-treated groups was significantly decreased. The CMU-treated groups showed significant decreases in Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia and demonstrated the colonizing ability of W. cibaria CMU in the oral cavity. We demonstrated that W. cibaria CMU suppresses halitosis, colonizes the oral cavity, and inhibits the proliferation of malodor-causing oral bacteria in beagles. According to these results, we expect that W. cibaria CMU could be a new oral hygiene solution by reducing VSC production and inhibiting the growth of oral harmful bacteria in companion animals.
KMT2D mutations and TP53 disruptions are poor prognostic biomarkers in mantle cell lymphoma receiving high-dose therapy: a FIL study

In recent years, the outcome of mantle cell lymphoma (MCL) has improved, especially in younger patients, receiving cytarabine-containing chemoimmunotherapy and autologous stem cell transplantation. Nevertheless, a proportion of MCL patients still experience early failure. To identify biomarkers anticipating failure of intensive chemotherapy in MCL, we performed target resequencing and DNA profiling of purified tumor samples collected from patients enrolled in the prospective FIL-MCL0208 phase 3 trial (high-dose chemoimmunotherapy followed by autologous transplantation and randomized lenalidomide maintenance). Mutations of KMT2D and disruption of TP53 by deletion or mutation associated with an increased risk of progression and death, both in univariate and multivariate analysis. By adding KMT2D mutations and TP53 disruption to the MIPI-c backbone, we derived a new prognostic index, the “MIPI-genetic” (“MIPI- g”). The “MIPI-g” improved the model discrimination ability compared to the MIPI-c alone, defining three risk groups: i) low-risk patients (4-year progression free survival and overall survival of 72.0% and 94.5%); ii) inter-mediate-risk patients (4-year progression free survival and overall survival of 42.2% and 65.8%) and iii) high-risk patients (4-year progression free survival and overall survival of 11.5% and 44.9%). Our results: i) confirm that TP53 disruption identifies a high-risk population characterized by poor sensitivity to conventional or intensified chemotherapy; ii) provide the pivotal evidence that patients harboring KMT2D mutations share the same poor outcome as patients harboring TP53 disruption; and iii) allow to develop a tool for the identification of high-risk MCL patients for whom novel therapeutic strategies need to be investigated. (Trial registered at clinicaltrials.gov identifier: NCT02354313).
Multimodal Imaging of Amyloid Plaques: Fusion of the Single-probe Mass Spectrometry Image and Fluorescence Microscopy Image.

Alzheimer's Disease (AD) is one of the most common neurodegenerative diseases. The formation of amyloid plaques by aggregated amyloid beta (Aβ) peptides is a primary event in AD pathology. Understanding the metabolomic features and related pathways are critical for studying plaque-related pathological events (e.g., cell death and neuron dysfunction). Mass spectrometry imaging (MSI), due to its high sensitivity and ability of obtaining spatial distribution of metabolites, has been applied to AD studies. How-ever, limited studies of metabolites in amyloid plaques were performed due to the drawbacks of commonly used techniques such as matrix-assisted laser desorption/ionization (MALDI) MSI. In the current study, we obtained high spatial resolution (~17 µm) MS images of AD mouse brain using the Single-probe, a microscale sampling and ionization device, coupled to a mass spectrome-ter under ambient conditions. The adjacent slices were used to obtain a fluorescence microscopy images to locate amyloid plaques. MS image and fluorescence microscopy image were fused to spatially correlate histological protein hallmarks with metabolomic features. The fused images produced significantly improved spatial resolution (~5 µm), allowing for the determination of fine structures in MS images and metabolomic biomarkers representing amyloid plaques.
Acetyl zingerone: An efficacious multifunctional ingredient for continued protection against ongoing DNA damage in melanocytes after sun exposure ends

Recent research has shown that significant levels of cyclobutane pyrimidine dimers (CPDs) in DNA continue to form in melanocytes for several hours in the dark after exposure to ultraviolet radiation (UVR) ends. We document the utility of a new multifunctional ingredient, 3‐(4‐hydroxy, 3‐methoxybenzyl)‐pentane‐2,4‐dione (INCI acetyl zingerone (AZ)), to protect melanocytes against CPD formation after UVR exposure ends.
Pivotal response treatment: A study into the relationship between therapist characteristics and fidelity of implementation

In this study, we investigated the relationship between fidelity of pivotal response treatment implementation and therapist characteristics, such as therapist personality, therapist–child relationship, therapist attitude toward evidence-based practices, and therapist experience. We also explored whether child age and autism symptom severity were related to pivotal response treatment fidelity. Participants were 41 level III certified pivotal response treatment therapists who recorded three 10-min pivotal response treatment sessions and completed four questionnaires to measure therapist characteristics. Results indicated that therapists’ attitude toward evidence-based practices, specifically openness to innovation, and therapists’ experience with pivotal response treatment significantly predicted fidelity of pivotal response treatment implementation. Cross-validation methods largely confirmed these findings. Therapist personality, therapist–child relationship, and child characteristics were not significantly related to pivotal response treatment fidelity. Implications for clinical practice and directions for future research are discussed. Lay abstract Pivotal response treatment is a naturalistic behavioral intervention that teaches pivotal skills to children with autism spectrum disorder to produce widespread gains in other skills. Although most children with autism spectrum disorder benefit from pivotal response treatment, intervention outcomes vary considerably among children. Fidelity of intervention implementation (i.e. the extent to which an intervention is implemented as intended) may affect intervention outcomes. In this study, we studied the relationship between fidelity of pivotal response treatment implementation and therapist characteristics, such as therapist personality, therapist–child relationship, therapist attitude toward evidence-based practices, and therapist experience. We also explored whether a child’s age and autism symptom severity were related to pivotal response treatment fidelity. Participants were 41 pivotal response treatment therapists who videotaped three pivotal response treatment sessions and completed four questionnaires to measure therapist characteristics. This study found that therapists’ openness to innovation and their experience with pivotal response treatment predicted fidelity of implementation. Therapist personality, therapist–child relationship, and child characteristics were not related to pivotal response treatment fidelity. The results of this study emphasize that it is important (1) to target therapists’ attitudes toward innovation prior to or during training in pivotal response treatment and (2) to provide therapists with ongoing supervision and feedback after training to increase fidelity of implementation and thus to improve intervention outcomes for children with autism spectrum disorder.
Cohort Profile Update: The Stockholm Birth Cohort Study (SBC)

The Stockholm Birth Cohort Study (SBC) was set up in 2004/2005 to investigate how childhood living conditions and experiences are related to life-course health, occupation and income. Briefly, the SBC was established by a probability matching of two longitudinal, anonymized datasets: the Stockholm Metropolitan Study (SMS) and the Swedish Work and Mortality Database (WMD). The former dataset contains information on all individuals who were born in 1953 and lived in the greater metropolitan area of Stockholm in 1963 (n1⁄4 15 117). Register and survey follow-ups were carried out up until 1986 when the SMS was de-identified. The latter dataset consists of all individuals who were born before 1985 and lived in Sweden in 1980 and/or 1990 (n1⁄4 9 428 526). The WMD includes information on income, work, education, family formation, in-patient care and mortality for the period 1981–2009. The probability matching rendered it possible for researchers to follow 14 294 members of the SMS cohort from birth (1953) to the age of 56 (2009).
Assessing waiting times in the clinical trajectory of patients with lung cancer

Lung cancer is among the most lethal and the most diagnosed cancers in Canada. Given the poor prognosis and symptom burden of the disease, timely access to treatment and quality care are essential. In spite of government commitments to reduce waiting times in cancer care, national clinical benchmarks for cancer care have yet to be established, and waiting time reporting by provinces is inconsistent. One of the proposed strategies for reducing waiting times in cancer care is the use of interdisciplinary teams. I undertook an audit of the interdisciplinary pulmonary oncology program at the Jewish General Hospital from 2004 to 2007. The primary objectives of this study were: (1) to audit the performance of the interdisciplinary pulmonary oncology service at the Jewish General Hospital with respect to waiting times for key intervals and subgroups of patients; (2) to compare waiting times in the clinical trajectory of lung cancer patients seen at the Jewish General Hospital with existing waiting time guidelines; (3) to determine those factors associated with longer waiting times in this population. A secondary objective was to suggest measures to be considered in order to reduce waiting times. The JGH’s lung cancer service was compared against benchmarks developed by the British Thoracic Society, Cancer Care Ontario, and the pan-Canadian waiting time benchmarks for radiation oncology. Patients waited a median of 9 days from the time they were ready to treat until their first treatment, and a median of 30 days from their first contact with the pulmonary service until their first treatment. Patients over age 65, those with early-stage disease and those with good performance status were less likely to meet the recommended guidelines.
(+)- and (−)-Spongiterpene, a pair of new valerenane sesquiterpene enantiomers from the marine sponge Spongia Sp.

Abstract (+)- and (−)-Spongiterpene [(+)-1 and (−)-1], a pair of new valerenane sesquiterpene enantiomers, along with four known compounds (2-5) were isolated from the marine sponge Spongia sp. The structures of (+)-1 and (−)-1 including absolute configurations were determined by spectroscopic analysis, quantum chemical calculation and X-ray diffraction. Compounds (+)-1 and (−)-1 were the first examples of valerenane sesquiterpenes isolated from the marine sponges. The cytotoxic activities of (+)-1 and (−)-1 were also evaluated. Graphical Abstract
Identification of QTLs for powdery mildew (Podosphaera aphanis; syn. Sphaerotheca macularis f. sp. fragariae) susceptibility in cultivated strawberry (Fragaria ×ananassa)

Strawberry powdery mildew (Podosphaera aphanis Wallr.) is a pathogen which infects the leaves, fruit, stolon and flowers of the cultivated strawberry (Fragaria ×ananassa), causing major yield losses, primarily through unmarketable fruit. The primary commercial control of the disease is the application of fungicidal sprays. However, as the use of key active ingredients of commercial fungicides is becoming increasingly restricted, interest in developing novel strawberry cultivars exhibiting resistance to the pathogen is growing rapidly. In this study, a mapping population derived from a cross between two commercial strawberry cultivars (‘Sonata’ and ‘Babette’) was genotyped with single nucleotide polymorphism (SNP) markers from the Axiom iStraw90k genotyping array and phenotyped for powdery mildew susceptibility in both glasshouse and field environments. Three distinct, significant QTLs for powdery mildew resistance were identified across the two experiments. Through comparison with previous studies and scrutiny of the F. vesca genome sequence, candidate genes underlying the genetic control of this trait were identified.
Low back pain in emergency ambulance workers in tertiary hospitals in China and its risk factors among ambulance nurses: a cross-sectional study

Objective Low back pain (LBP) could cause serious consequences and has been shown to be prevalent among emergency ambulance workers. Studies on the prevalence of and risk factors for LBP among emergency ambulance workers are scarce in China. The study aimed to determine the prevalence of LBP among ambulance workers, including doctors, nurses and drivers, and to explore the risk factors for ambulance nurses’ chronic LBP (lasting for at least 3 months). Design Cross-sectional study. Setting Emergency ambulance systems from 38 tertiary hospitals in Shandong, China were selected by random cluster sampling. Participants A total of 1560 ambulance workers completed the study. Outcome measures A paper-based questionnaire that included the Nordic Musculoskeletal Questionnaire, which evaluated LBP, the Dutch Musculoskeletal Questionnaire, which assessed ergonomic factors, and the Job Content Questionnaire, which assessed stress, was used. Multivariate logistic regression analysis was conducted to quantify the association of potential risk factors with chronic LBP among ambulance nurses. Results The 1 year prevalence of LBP lasting for at least 24 hours, 7 days and 3 months was 86.1%, 50.6% and 21.1%, respectively, among 498 ambulance nurses; 70.5%, 36.4% and 15.8% among 519 doctors; and 57.5%, 23.8% and 12.3% among 543 drivers. The factors contributing to chronic LBP among ambulance nurses were the frequent bending of the trunk, heavy or awkward lifting, shift work, low job satisfaction, high psychological fatigue, high psychological job demand, low job control, low supervisor support, older age, female sex and obesity. Conclusions LBP was more prevalent among ambulance nurses than among ambulance doctors and drivers. Many factors, especially psychosocial and ergonomic factors, contributed to ambulance nurses’ chronic LBP. Comprehensive measures might be needed to control LBP.
Preparation and comparative stability of a kaolinite-tetrabutylphosphonium bromide intercalation compound for heat and solvent treatments.

A kaolinite-tetrabutylphosphoium bromide (TBPBr) intercalation compound (Kaol-TBPBr) was prepared based on kaolinite to provide inorganic aluminosilicate layers and TBPBr as intercalated salts between the layers through the use of an intermediate, a kaolinite-dimethylsulfoxide (DMSO) intercalation compound (Kaol-DMSO). The experimental data through complementary techniques, including X-ray diffraction (XRD), Fourier transform infrared (FT-IR), solid-state 13C and 29Si nuclear magnetic resonance (NMR) with cross polarization and magic angle spinning (13C and 29Si CP/MAS NMR), inductively-coupled plasma (ICP) emission spectrometry and ion chromatography (IC), indicated full removal of DMSO and intercalation of TBPBr with an increase in the basal spacing from 1.12 (Kaol-DMSO) to 1.53 nm (Kaol-TBPBr). In contrast to a similar intercalation compound, a kaolinite-tetrabutylammonium bromide (TBABr) intercalation compound (Kaol-TBABr) with basal spacing of 1.51 nm, Kaol-TBPBr displayed interesting features such as enhanced thermal properties as well as bold resistance against several solvents. Kaol-TBPBr withstood thermal decomposition of the organic species over 100 C much better than Kaol-TBABr. When Kaol-TBPBr and Kaol-TBABr were refluxed in methanol, ethanol, acetone or toluene for 1 day, Kaol-TBPBr preserved the expanded kaolinite layers, while the Kaol-TBABr structure completely collapsed due to release of TBABr. Thus, with these particular and unique features of Kaol-TBPBr, organophosphonium salts appear to be promising guest species for intercalation chemistry of kaolinite.
Association of Untargeted Urinary Metabolomics and Lung Cancer Risk Among Never-Smoking Women in China

Key Points Question What is the association of metabolomic biomarkers with lung cancer in women who have never smoked? Findings In this case-control study of 275 never-smoking women with lung cancer and 289 never-smoking cancer-free women in China, the metabolite 5-methyl-2-furoic acid was correlated with dietary soy consumption and pathways including 1-carbon metabolism, oxidative stress, nucleotide metabolism, and inflammation and was significantly associated with lower lung cancer risk. Meaning These findings suggest that certain metabolites and pathways are associated with lower lung cancer risk in never-smoking women and biological processes linked to air pollution may be associated with higher lung cancer risk in this population.
Ablative brain surgery: an overview

Abstract Background: Ablative therapies have been used for the treatment of neurological disorders for many years. They have been used both for creating therapeutic lesions within dysfunctional brain circuits and to destroy intracranial tumors and space-occupying masses. Despite the introduction of new effective drugs and neuromodulative techniques, which became more popular and subsequently caused brain ablation techniques to fall out favor, recent technological advances have led to the resurgence of lesioning with an improved safety profile. Currently, the four main ablative techniques that are used for ablative brain surgery are radiofrequency thermoablation, stereotactic radiosurgery, laser interstitial thermal therapy and magnetic resonance-guided focused ultrasound thermal ablation. Object: To review the physical principles underlying brain ablative therapies and to describe their use for neurological disorders. Methods: The literature regarding the neurosurgical applications of brain ablative therapies has been reviewed. Results: Ablative treatments have been used for several neurological disorders, including movement disorders, psychiatric disorders, chronic pain, drug-resistant epilepsy and brain tumors. Conclusions: There are several ongoing efforts to use novel ablative therapies directed towards the brain. The recent development of techniques that allow for precise targeting, accurate delivery of thermal doses and real-time visualization of induced tissue damage during the procedure have resulted in novel techniques for cerebral ablation such as magnetic resonance-guided focused ultrasound or laser interstitial thermal therapy. However, older techniques such as radiofrequency thermal ablation or stereotactic radiosurgery still have a pivotal role in the management of a variety of neurological disorders.
Cumulative exposure to ionising radiation from diagnostic imaging tests: a 12-year follow-up population-based analysis in Spain

Objectives To calculate each patient’s cumulative radiation exposure and the recurrent tests during a 12-year study period, according to sex and age, in routine practice. Design Retrospective cohort study. Setting A general hospital with a catchment population of 224 751 people, in the Southeast of Spain. Participants Population belonged to the catchment area of that hospital in 2007. We collected all consecutive diagnostic imaging tests undergone by this population until 31 December 2018. We excluded: imaging tests that did not involve radiation exposure. Main outcome measures The cumulative effective dose and the recurrent imaging tests by sex and age at entry of study. Results Of the 224 751 people, 154 520 (68.8%) underwent an imaging test. The population had 1 335 752 imaging tests during the period of study: 1 110 077 (83.0%) plain radiography; 156 848 (11.8%) CT; 63 157 (4.8%) fluoroscopy and 5670 (0.4%) interventional radiography. 25.4% of the patients who had a CT, underwent five or more CTs (5.4% in the 0–20 years age group). The median total cumulative effective dose was 2.10 mSv (maximum 3980.30) and 16.30 mSv (maximum 1419.30 mSv) if we considered only doses associated with CT. Women received more effective dose than men (median 2.38 vs median 1.90, p<0.001). A total of 7142 (4.6%) patients received more than 50 mSv, with differences in men and women (p<0.001) and 2.5% of the patients in the 0–20 years age group, if we considered only doses associated with CT. Conclusions Nearly 5% of patients received doses higher than 50 mSv during the 12-year period of study and 2.5% of the patients in the 0–20 years age group, if we considered only doses associated with CT. The rate of recurrent examinations was high, especially in older patients, but also relevant in the 0–20 years age group.