Miracle-dz/UniFAR-FTUs huggingface dataset:
THE ACTIVITY OF ALKALINE AND ACID PHOSPHATASE IN THE HUMAN PLACENTA

IT is generally accepted that in the placenta, as in other tissues, the acid and alkaline phosphatases are concerned with the metabolism of carbohydrates and phospholipids (see references in Long, 1961). These enzymes are also thought to be essential for the active transport of some intermediary metabolites across the placenta. Biochemical determinations of phosphatase activity in the human placenta have shown that alkaline phosphatase activity rises throughout pregnancy and that acid phosphatase activity tends to decline gradually from 10-weeks gestation to term (McKay et al., 1958). Extensive histochemical studies of the distribution and amount of these enzymes in the human placenta have been made at various stages of pregnancy, but these studies have not provided any general agreement about the amounts of either enzyme present in placentae from normal or abnormal pregnancies (Dempsey and Wislocki, 1945; Wislocki and Dempsey, 1946, 1948; Botella and Cano, 1950; Hughes et al., 1954; Thomsen, 1955a, b; Dumont, 1957; McKay et al., 1958; Pazourek, 1958; Lobe1 et al., 1962). This confusion may result from the fact that histochemical methods are based on the examination of only small and possibly unrepresentative portions of the whole placenta. The present study was therefore undertaken: acid and alkaline phosphatase activity was estimated in extracts of the whole placenta in order to determine if the activities of these enzymes differed from normal in placentae associated with toxaemia of pregnancy, intrauterine death, and other abnormal conditions. IY.C. 2
Spontaneous Pubertal Development in Turner’s Syndrome

The incidence of spontaneous puberty in Turner's syndrome is reported to be between 5-10% and, more recently in some series, as high as 20%. In an Italian retrospective multicenter study, of 522 patients older than 12 yr with Turner's syndrome, 84 patients (16, 1%) presented spontaneous pubertal development with menarche that occurred at a chronological age of 13.2 +/- 1.5 yr (mean +/- SD) and a bone age of 12.9 +/- 1.9 yr. Karyotype distribution in the whole group was as follows: 52.1% (272 patients) X-monosomy (45,X), 13.2% (69 patients) mosaicism characterized by X-monosomy and cellular line with no structural abnormalities of the second X, 19.9% (104 patients) mosaicism characterized by X-monosomy and cellular line with structural abnormalities of the second X, and 14.8% (77 patients) structural abnormalities of the second X. Menstrual cycles were still regular in 30 patients at 9.2 +/- 5.0 yr after menarche, 12 developed secondary amenorrhea 1.6 +/- 2.0 yr after menarche, and 19 had irregular menstrual cycles 0.9 +/- 1.8 yr after menarche. As signs of spontaneous puberty developed in 14.0% of X-monosomic patients and in 32.0% of patients with cell lines with more than one X, the presence of the second X seems to have a cardinal influence on the appearance of spontaneous puberty. Spontaneous pregnancy occurred in 3 patients (3.6%). The presence of chromosomal abnormalities and malformations in 2 of 3 pregnancies led us to agree with other investigators in discouraging unassisted pregnancies. Treatment with GH does not seem to exert any influence on either the age of onset or the prevalence of spontaneous pubertal development in Turner's syndrome. The increased percentage of spontaneous menarche is Turner's syndrome reported in the recent literature might be due to increased ascertainment by diligent screening for Turner's syndrome in girls with short stature and mild or no Turner's syndrome stigmata, even though they may be menstruating.
The safety of ECT in patients with chronic obstructive pulmonary disease.

BACKGROUND Electroconvulsive therapy (ECT) involves the administration of general anesthesia and assisted ventilation while the patient is apneic. OBJECTIVE Care must be taken to screen for significant pulmonary dysfunction before treatment. Very little has been written about the safety and management strategy of ECT patients with chronic obstructive pulmonary disease (COPD). METHOD In this retrospective chart review, authors describe their experience with patients in recent years who had this disorder and were treated with ECT. RESULTS Authors list recommendations for the pre-ECT work up and anesthetic management during and after the treatments. CONCLUSION Recent guidelines recommend administration of patients' prescribed inhalers on the morning of ECT treatment. Also, caution is recommended when using ECT in patients taking theophylline because this drug has been associated with prolonged seizures and status epilepticus in these patients.
Endemism in the moss flora of North America.

PREMISE OF THE STUDY Identifying regions of high endemism is a critical step toward understanding the mechanisms underlying diversification and establishing conservation priorities. Here, we identified regions of high moss endemism across North America. We also identified lineages that contribute disproportionately to endemism and document the progress of efforts to inventory the endemic flora. METHODS To understand the documentation of endemic moss diversity in North America, we tabulated species publication dates to document the progress of species discovery across the continent. We analyzed herbarium specimen data and distribution data from the Flora of North America project to delineate major regions of moss endemism. Finally, we surveyed the literature to assess the importance of intercontinental vs. within-continent diversification for generating endemic species. KEY RESULTS Three primary regions of endemism were identified and two of these were further divided into a total of nine subregions. Overall endemic richness has two peaks, one in northern California and the Pacific Northwest, and the other in the southern Appalachians. Description of new endemic species has risen steeply over the last few decades, especially in western North America. Among the few studies documenting sister species relationships of endemics, recent diversification appears to have played a larger role in western North America, than in the east. CONCLUSIONS Our understanding of bryophyte endemism continues to grow rapidly. Large continent-wide data sets confirm early views on hotspots of endemic bryophyte richness and indicate a high rate of ongoing species discovery in North America.
Knowledge and attitudes of nursing staff and mothers towards kangaroo mother care in the eastern sub-district of Cape Town

Abstract Objectives: To determine the knowledge and attitude of nursing staff and mothers towards kangaroo mother care (KMC) in the eastern sub-district of Cape Town. Design: A cross-sectional descriptive study. Setting and subjects: A multi-stage sample of 30 kangaroo care mothers admitted to the Helderberg District Hospital (HDH); six nurses from the HDH; and nine nurses from the seven antenatal clinics that the mothers attended. The respondents were interviewed using a pretested questionnaire. Outcome measures: Knowledge, attitudes and acceptability of KMC. Results: Data were analysed using CDC Epi Info version 3.3. 2, and Microsoft Excel software programmes. The majority of the mothers (83.3%) did not have prior knowledge of KMC. Sixty per cent of the nursing staff did not have any KMC training. The majority of the mothers were committed to KMC, were satisfied with the results (with regard to the weight gain of the infant), and indicated that they would continue to practise KMC at home. The majority of the hospital nursing staff was very positive toward KMC, and agreed that it was beneficial to both mother and infant. Conclusion: Most of the mothers lacked prior knowledge of KMC, and were only informed about it when they were admitted to the KMC ward. All of the nursing staff who were engaged in KMC (n = 15) had a positive attitude towards it.
The relationship among television watching, physical activity, and body composition of 5- or 6-year-old children

This follow-up investigation examined the relationship among observed time of television watching, physical activity, and body composition in 5- to 6-year-old children previously studied 2 years ago. Activity level on school and nonschool days was measured with the Children’s Activity Rating Scale. Television watching time was assessed by direct observation, and body composition was measured with the body mass index, skinfold thicknesses, and waist/hip ratio. Television watching behavior, which increased from the earlier study, was not associated with body composition. Physical activity was lower during television watching than nontelevision watching time.
Differences in postprandial protein handling after beef compared with milk ingestion during postexercise recovery: a randomized controlled trial.

BACKGROUND Protein consumed after resistance exercise increases postexercise muscle protein synthesis rates. To date, dairy protein has been studied extensively, with little known about the capacity of other protein-dense foods to augment postexercise muscle protein synthesis rates. OBJECTIVE We aimed to compare protein digestion and absorption kinetics, postprandial amino acid availability, anabolic signaling, and the subsequent myofibrillar protein synthetic response after the ingestion of milk compared with beef during recovery from resistance-type exercise. DESIGN In crossover trials, 12 healthy young men performed a single bout of resistance exercise. Immediately after cessation of exercise, participants ingested 30 g protein by consuming isonitrogenous amounts of intrinsically l-[1-(13)C]phenylalanine-labeled beef or milk. Blood and muscle biopsy samples were collected at rest and after exercise during primed continuous infusions of l-[ring-(2)H5]phenylalanine and l-[ring-3,5-(2)H2]tyrosine to assess protein digestion and absorption kinetics, plasma amino acid availability, anabolic signaling, and subsequent myofibrillar protein synthesis rates in vivo in young men. RESULTS Beef protein-derived phenylalanine appeared more rapidly in circulation compared with milk ingestion (P < 0.001). The availability of phenylalanine during the 5-h postexercise period tended to be higher after beef (64% ± 3%) ingestion than after milk ingestion (57% ± 3%; P = 0.08). Both beef and milk ingestion were followed by an increase in the phosphorylation of mammalian target of rapamycin complex 1 and 70-kDa S6 protein kinase 1 during postexercise recovery. Milk ingestion increased myofibrillar protein synthesis rates to a greater extent than did beef ingestion during the 0- to 2-h postexercise phase (P = 0.013). However, the increase in myofibrillar protein synthesis rates did not differ between milk and beef ingestion during the entire 0- to 5-h postexercise phase (P = 0.114). CONCLUSIONS Both milk and beef ingestion augment the postexercise myofibrillar protein synthetic response in young men, with a stronger stimulation of myofibrillar protein synthesis during the early postprandial stage after milk ingestion. This trial was registered at www.clinicaltrials. gov as NCT01578590.
Radioprotection of the Brain White Matter by Mn(III) N-Butoxyethylpyridylporphyrin–Based Superoxide Dismutase Mimic MnTnBuOE-2-PyP5+

Cranial irradiation is a standard therapy for primary and metastatic brain tumors. A major drawback of radiotherapy (RT), however, is long-term cognitive loss that affects quality of life. Radiation-induced oxidative stress in normal brain tissue is thought to contribute to cognitive decline. We evaluated the effectiveness of a novel mimic of superoxide dismutase enzyme (SOD), MnTnBuOE-2-PyP5+(Mn(III) meso-tetrakis(N-n-butoxyethylpyridinium-2-yl)porphyrin), to provide long-term neuroprotection following 8 Gy of whole brain irradiation. Long-term RT damage can only be assessed by brain imaging and neurocognitive studies. C57BL/6J mice were treated with MnTnBuOE-2-PyP5+ before and after RT and evaluated three months later. At this time point, drug concentration in the brain was 25 nmol/L. Mice treated with MnTnBuOE-2-PyP5+/RT exhibited MRI evidence for myelin preservation in the corpus callosum compared with saline/RT treatment. Corpus callosum histology demonstrated a significant loss of axons in the saline/RT group that was rescued in the MnTnBuOE-2-PyP5+/RT group. In addition, the saline/RT groups exhibited deficits in motor proficiency as assessed by the rotorod test and running wheel tests. These deficits were ameliorated in groups treated with MnTnBuOE-2-PyP5+/RT. Our data demonstrate that MnTnBuOE-2-PyP5+ is neuroprotective for oxidative stress damage caused by radiation exposure. In addition, glioblastoma cells were not protected by MnTnBuOE-2-PyP5+ combination with radiation in vitro. Likewise, the combination of MnTnBuOE-2-PyP5+ with radiation inhibited tumor growth more than RT alone in flank tumors. In summary, MnTnBuOE-2-PyP5+ has dual activity as a neuroprotector and a tumor radiosensitizer. Thus, it is an attractive candidate for adjuvant therapy with RT in future studies with patients with brain cancer. Mol Cancer Ther; 14(1); 70–79. ©2014 AACR.
MTHFR 677C->T genotype is associated with folate and homocysteine concentrations in a large, population-based, double-blind trial of folic acid supplementation.

BACKGROUND The methylenetetrahydrofolate reductase (MTHFR) genotype is associated with modification of disease and risk of neural tube defects. Plasma and red blood cell (RBC) folate and plasma homocysteine concentrations change in response to daily intakes of folic acid supplements, but no large-scale or population-based randomized trials have examined whether the MTHFR genotype modifies the observed response. OBJECTIVE We sought to determine whether the MTHFR 677C→T genotype modifies the response to folic acid supplementation during and 3 mo after discontinuation of supplementation. DESIGN Northern Chinese women of childbearing age were enrolled in a 6-mo supplementation trial of different folic acid doses: 100, 400, and 4000 μg/d and 4000 μg/wk. Plasma and RBC folate and plasma homocysteine concentrations were measured at baseline; after 1, 3, and 6 mo of supplementation; and 3 mo after discontinuation of supplementation. MTHFR genotyping was performed to identify a C→T mutation at position 677 (n = 932). RESULTS Plasma and RBC folate and homocysteine concentrations were associated with MTHFR genotype throughout the supplementation trial, regardless of folic acid dose. MTHFR TT was associated with lower folate concentrations, and the trend of TT < CC was maintained at even the highest doses. Folic acid doses of 100 μg/d or 4000 μg/wk did not reduce high homocysteine concentrations in those with the MTHFR TT genotype. CONCLUSION MTHFR genotype was an independent predictor of plasma and RBC folate and plasma homocysteine concentrations and did not have a significant interaction with folic acid dose during supplementation. This trial was registered at clinicaltrials.gov as NCT00207558.
Amniocentesis dilemma : Needs assessment, development and field-testing of a theory-based decision support intervention

Background: Amniocentesis is the most common prenatal diagnostic procedure undertaken in the United Kingdom, usually performed after 15 completed weeks of pregnancy. The procedure is reported to have a 1 % risk of miscarriage and the results of the chromosome tests may require further decision making about whether to continue with the pregnancy. Deciding about amniocentesis is a complex and emotionally charged decision, often undertaken in a short period of time and, under current practice, with little systematic decision support. Decision Support Interventions, also known as Patient Decision Aids, have been developed to help individuals leam about the features and implications of their treatment or screening options while improving communication with their health professionals. Those interventions are specifically targeted at preference-sensitive decisions with significant harms, benefits and uncertainty, where no screening or treatment option is objectively better than the other. This thesis proposed to assess information and decision support needs of pregnant women undertaking amniocentesis testing and to design and field-test, in collaboration with pregnant women and health professionals, a theory-based Decision Support Intervention for amniocentesis testing (amnioDex). Methods: A multi-method approach was adopted that included a systematic review, theoretical review, and qualitative analysis to develop and pilot a theory-based intervention intended for pregnant women facing a decision to undertake amniocentesis testing. The content areas and themes to be covered in the intervention were determined by a literature review and needs assessment conducted with pregnant women and health professionals. The prototype development of amnioDex (amniocentesis decision explorer) was guided by theory and included heuristic-based deliberation tools. Incremental prototypes of amnioDex and embedded deliberation tools were field-tested with lay users, health professionals and pregnant women facing a decision to undertake amniocentesis, using the "think-aloud" technique. Results: The amnioDex intervention was developed over a period of two years and field-tested for eight months. Conclusion: Findings from this thesis showed that it was feasible to use theory to generate a Decision Support Intervention acceptable to women facing amniocentesis testing and to health professionals counselling them. Future research needs to evaluate the effectiveness of amnioDex in a randomised controlled trial and to examine methods for effectively transferring theory into practice.
The Impact of the in utero and Early Postnatal Environments on Grey and White Matter Volume: A Study with Adolescent Monozygotic Twins

Prenatal and early postnatal adversities have been shown to be associated with brain development. However, we do not know how much of this association is confounded by genetics, nor whether the postnatal environment can moderate the impact of in utero adversity. This study used a monozygotic (MZ) twin design to assess (1) the association between birth weight (BW) and brain volume in adolescence, (2) the association between within-twin-pair BW discordance and brain volume discordance in adolescence, and (3) whether the association between BW and brain volume in adolescence is mediated or moderated by early negative maternal parenting behaviours. These associations were assessed in a sample of 108 MZ twins followed longitudinally since birth and scanned at age 15. The total grey matter (GM) and white matter (WM) volumes were obtained using the Diffeomorphic Anatomical Registration Through Exponentiated Lie Algebra (DARTEL) toolbox in the Statistical Parametric Mapping version 8 (SPM8). We found that the BW was significantly associated with the total GM and WM volumes, particularly in the superior frontal gyrus and thalamus. Within-twin-pair discordance in BW was also significantly associated with within-pair discordance in both the GM and the WM volumes, supporting the hypothesis that the specific in utero environment is associated with brain development independently of genetics. Early maternal hostile parenting behaviours and depressive symptoms were associated with total GM volume but not WM volume. Finally, greater early maternal hostility may moderate the association between the BW and GM volume in adolescence, since the positive association between the BW and total GM volume appeared stronger at higher levels of maternal hostility (trend). Together, these findings support the importance of the in utero and early environments for brain development.
The relationship between parents’ first concerns and symptoms of autism spectrum disorders

Objective: To examine the relationship between parents’ first concerns and early Autism Spectrum Disorder (ASD) symptoms. Methods: Symptoms of ASD were compared in 1393 toddlers with and without a diagnosis of an ASD, based on the area of parents’ first concerns. Communication and behaviour problems were examined in the current study, as they are the most frequently reported first concerns in the literature. A series of one-way, between-subjects ANOVAs were conducted on each sub-scale of the BISCUIT Part-1. Results: Symptoms of Autism Spectrum Disorders (ASD) significant differences were found between most groups on all sub-scales. On the Socialization/Non-verbal Communication and Repetitive Behaviour/Restricted Interest sub-scales, those with ASD and behaviour concerns had the highest scores. On the Communication sub-scale, those with ASD and communication concerns had the highest scores. Conclusions: A significant relationship exists between early ASD symptoms and area of first concern. The implications of these results are discussed.
Evaluación del control de calidad de la baciloscopia en el diagnóstico de la tuberculosis en Cuba

Se evaluo el diagnostico de tuberculosis en los laboratorios de los Centros Provinciales de Higiene y Epidemiologia de Cuba incluido el municipio especial Isla de la Juventud. Se realizo el control de calidad a 4 382 laminas en el periodo enero-diciembre de 2004, segun lo establecido en el Manual de Procedimientos del Programa Nacional de Control de Tuberculosis. Mostraron buena calidad en la extension y coloracion de Zielh Neelsen 4 003 (91,35 %) laminas. El coeficiente de concordancia global del Laboratorio Nacional de Referencia de Tuberculosis en relacion con los laboratorios provinciales fue de 99,79 %. La sensibilidad y especificidad presentaron valores superiores a 99 %. Estos resultados sugieren la calidad del diagnostico de la baciloscopia de tuberculosis en los laboratorios provinciales y senalan la necesidad de no descuidar las continuas supervisiones, el monitoreo y el reentrenamiento en la tecnica de baciloscopia, que sientan las bases para la introduccion de los metodos de rechequeo a ciegas y panel de laminas dirigidas a perfeccionar la calidad del diagnostico de tuberculosis en Cuba.
Care Provision in Housing With Supportive Services: The Importance of Care Type, Individual Characteristics, and Care Site

Housing with supportive services is an important long-term sector, but information about care provision to residents in these settings is largely unavailable. The role of individual characteristics versus facility identity in determining how care is provided is completely unexplored. Data from 60 facilities in a single state were used to investigate the degree to which individual characteristics and facility identity determined how much care was provided to residents. Individual characteristics had the greatest impact on the amount of direct care time received by individuals. Care that was dementia oriented (i.e., cueing), however, was more strongly affected by the identity of the facility than by individual characteristics. These results have important implications for how consumers should think about seeking, and policy makers should think about supporting, care for those with impaired cognitive status who utilize housing with supportive services.