Miracle-dz/UniFAR-FTUs huggingface dataset:
Synergistic O3 + OH oxidation pathway to extremely low-volatility dimers revealed in β-pinene secondary organic aerosol

Significance Secondary organic aerosol (SOA) is ubiquitous in the atmosphere and plays a pivotal role in climate, air quality, and health. Monoterpenes, emitted in large quantities from forested regions, are a dominant source of SOA globally, with dimers having been identified as key contributors to particle formation and growth. Here, we establish the role of concerted oxidation by O3 and OH as a significant route to dimer formation in SOA generated from β-pinene, the second-most-abundant monoterpene emitted to the atmosphere. Production of this class of dimers is found to occur through both gas- and particle-phase processes. Dimer formation via synergistic O3 + OH oxidation could represent an appreciable source of “missing” SOA not included in current atmospheric models. Dimeric compounds contribute significantly to the formation and growth of atmospheric secondary organic aerosol (SOA) derived from monoterpene oxidation. However, the mechanisms of dimer production, in particular the relevance of gas- vs. particle-phase chemistry, remain unclear. Here, through a combination of mass spectrometric, chromatographic, and synthetic techniques, we identify a suite of dimeric compounds (C15–19H24–32O5–11) formed from concerted O3 and OH oxidation of β-pinene (i.e., accretion of O3- and OH-derived products/intermediates). These dimers account for an appreciable fraction (5.9–25.4%) of the β-pinene SOA mass and are designated as extremely low-volatility organic compounds. Certain dimers, characterized as covalent dimer esters, are conclusively shown to form through heterogeneous chemistry, while evidence of dimer production via gas-phase reactions is also presented. The formation of dimers through synergistic O3 + OH oxidation represents a potentially significant, heretofore-unidentified source of low-volatility monoterpene SOA. This reactivity also suggests that the current treatment of SOA formation as a sum of products originating from the isolated oxidation of individual precursors fails to accurately reflect the complexity of oxidation pathways at play in the real atmosphere. Accounting for the role of synergistic oxidation in ambient SOA formation could help to resolve the discrepancy between the measured atmospheric burden of SOA and that predicted by regional air quality and global climate models.
Overexpression of dedicator of cytokinesis 2 correlates with good prognosis in colorectal cancer associated with more prominent CD8+ lymphocytes infiltration: a colorectal cancer analysis

Recently, dedicator of cytokinesis 2 (DOCK2) has been reportedly exhibited high mutation prevalence in the Asian colorectal cancer (CRC) cohort. However, the expression pattern of DOCK2 and its clinical significance in CRC were still unknown. To characterize the role of DOCK2, a tissue microarray (TMA) containing 481 archived paraffin‐embedded CRC specimens was performed by immunohistochemistry. Among which, 54 primary CRC tissues showed high expression of DOCK2 protein, while others were negative. Moreover, DOCK2 expression was positively associated with invasion depth (P < .001) and tumor size (P = .016). Significantly, Kaplan‐Meier survival analysis revealed that patients with higher DOCK2 expression had a longer overall survival time (P = .017). Furthermore, univariate and multivariate Cox regression analysis confirmed that DOCK2 is an independent prognostic marker in CRC (P = .049,; HR, 0.519; 95% CI, 0.270 to 0.997). In addition, we observed a strong correlation between the infiltration of CD8+ T lymphocytes and DOCK2 expression (P = .0119). Our findings demonstrated that overexpressed DOCK2 might involve in recruiting CD8+ T lymphocytes and serve as a novel prognostic indicator and indicated a potential therapeutic strategy by restoring DOCK2 for CRC.
(2R,3R)Dihydromyricetin inhibits osteoclastogenesis and bone loss through scavenging LPS‐induced oxidative stress and NF‐κB and MAPKs pathways activating

Osteolysis is a serious complication of several chronic inflammatory diseases and is closely associated with a local chronic inflammatory reaction with a variety of causes. However, similarities exist in the mechanisms of their pathological processes. Inflammatory factors and oxidative stress–induced nuclear factor κB (NF‐κB) and mitogen‐activated protein kinases (MAPKs) signaling pathways play a center role in bone erosion. Dihydromyricetin (DMY) is a natural compound with anti‐inflammatory and antioxidative effect, which are commonly used in chronic pharyngitis and alcohol use disorders. In the current study, we identified that DMY attenuated lipopolysaccharide (LPS)–induced oxidative stress through inhibiting the production of reactive oxygen species (ROS) and nitric oxide (NO), downregulated COX‐2 and iNOS, and promoted the activity of the antioxidative system by activating superoxide dismutase (SOD) and Nrf2/HO‐1 pathway. To further investigate the underlying mechanism, we found that DMY inhibits osteoclast (OC) differentiation and bone resorption activity through blocking the RANKL‐induced activation of the NF‐κB and MAPKs signaling pathways and then downregulated c‐Fos and NFATc1, which is essential for OC differentiation. Furthermore, DMY inhibited LPS‐induced osteolysis in vivo. Collectively, these results indicate that DMY might be a promising prophylactic antiosteoclastic/resorptive agent in preventing or treating bone lysis diseases.
Urticaria in children and adolescents: An updated review of the pathogenesis and management

The present survey represents the latest data on diagnosis and management of childhood urticaria. It has been observed that urticaria occurs less often in children than adults, with symptoms rarely lasting for over 6 weeks. Triggers or aggravating factors can be found only in 21%‐55% of cases. Finding autoantibodies in children does not impact a disease prognosis, unlike in adult patients, where the presence of autoantibodies is associated with a more prolonged run of the disease, a more severe prognosis and more intensive treatment methods. The incidence of food allergy equals to 8%‐10% of cases. The incidence of Helicobacter Pylori infection in children is lower than that in adults and comes to 10%‐18%. Medical experts recommend using the same treatment schemes for adults and children. This survey describes different urticaria management patterns suggested by experts from Europe, America, and Russia in their recent guidelines. It has been noted that unlike the guidelines from 2014, the 2018 clinical practice guidelines for the diagnosis and management of urticarial once again suggest a four‐step treatment scheme with assigning omalizumab for Step 3 and cyclosporine A for Step 4 in the event of low therapeutic efficacy of the previous step or its impossibility. Leukotriene antagonists (LTRAs) are currently removed from basic management to alternative programs.
Ultrastructure of Euspermatozoa of cerithiacean gastropods (Prosobranchia: Mesogastropoda)

Euspermatozoa of selected cerithiacean gastropods have been studied using transmission electron microscopy and the results compared (primarily) with previous studies of mesogastropod and neogastropod euspermatozoa. Cerithiacean euspermatozoa each possess a well‐defined acrosome (extremely varied in shape), a short (2.25—3 μm), very electron‐dense nucleus, an elongate midpiece, and an elongate glycogen piece. A dense ring structure associated with the plasma membrane occurs at the junction of the midpiece and glycogen piece. While features such as the dense ring structure and the systematic periaxonemal arrangement of “glycogen” granules can be understood from a purely functional standpoint, it is suggested that euspermatozoon features also provide information of taxonomic and phylogenetic relevance. On the basis of euspermatozoon midpiece structure, true cerithiaceans can be easily distinguished from other mesogastropods and from neogastropods and are divided tentatively into two groups: Group 1 (Turritellidae, Cerithiidae, Australaba (family uncertain), Planaxidae, Potamididae (subfamily Batillariinae)), and Group 2 (Potamididae (subfamily Potamidinae), Modulidae, Obtortio (family uncertain)). Using midpiece and acrosomal features, group 1 can be further subdivided into two subgroups: Subgroup 1(i) (Turritellidae, Cerithiidae, Australaba) and Subgroup 1(ii) (Planaxidae, Potamididae (subfamily Batillariinae)). It is suggested that the pronounced differences existing between the two subfamilies of the Potamididae may indicate the necessity for a separate family for the Batillariinae.
Strategies to improve menopausal quality of life: A systematic review

BACKGROUND: Menopause and its complications could disturb the sense of well-being and health and affect the quality of life. The present study was performed to review the conducted interventional study related to the quality of life in menopausal women in Iran. METHODS: In the present systematic review, to achieve the intended studies, Iranian Registry for Clinical Trials and Magiran, SID, Google Scholar, Scopus, PubMed, Proquest, ScienceDirect, and Web of Science databases were searched using: menopause” and “quality of life” keywords without any time limitations. Based on Jadad criteria, studies with a score of 3 or more were enrolled in the study. RESULTS: From all the achieved studies at primary search, 12 were selected and enrolled in the study. Reviewing the results of the studies showed that participating physical exercise, using products containing phytoestrogens and isoflavones and participating in educational and counseling sessions have an effective role in the improvement of quality of life in menopausal women. CONCLUSIONS: Evidence indicated that from the existing strategies to improve the menopausal quality of life, using complementary medicine is an efficient method and could be more effective when consumed along with performing physical exercises and participating in educational programs.
Magnetic Resonance Measurements of Sacrococcygeal and Intercoccygeal Angles in Normal Participants and those with Idiopathic Coccydynia

Background In the past, few studies have been done to objectively measure the sacrococcygeal (SC) and intercoccygeal (IC) angles in the population and in patients with coccydynia. Coccydynia is an age-old disorder, the exact incidence of which has not been determined. It is reported to be more common in females and the obese. The magnetic resonance imaging (MRI) studies done in the past have calculated the curvature indices. In this study, we used MRI to objectively measure the angles in the normal participants as well as those with idiopathic coccydynia Materials and Methods Two groups of patients were identified. Group A was “control group” of 106 normal participants and Group B comprised “study group” of ten patients suffering from idiopathic coccydynia. In all these patients, midsagittal T1-weighted MRI image acquired in supine position was used to calculate SC and IC angles. Data were analyzed, and angles were compared between the study and control groups. Statistical analysis was done with Chi-square test Results In the control group, the average SC and IC angles in the control group were 126.8° and 33.5°, respectively. In the study group, the average SC angle and the average IC angle turned out to be 127.1° and 43.2°, respectively. The difference between the SC angles in the control and study groups was not significant ( P = 0.7), whereas the difference between the IC angles in the two groups was significant ( P = 0.002) Conclusions From our study, we observed that the IC angle shows a decreasing trend with increasing age. In addition, increased IC angle was identified as a possible cause of idiopathic coccydynia
Breast self-examination awareness and practices in young women in developing countries: A survey of female students in Karachi, Pakistan

BACKGROUND: Breast cancer is a leading cause of deaths worldwide. In Pakistan, one in every nine women develops breast cancer some time in her life. This high incidence of breast cancer and the poor socioeconomic conditions of Pakistan make breast self-examination (BSE) the best tool for the prevention and early diagnosis of breast cancer. Therefore, the aim of the study was to assess the knowledge, attitude, and practice of BSE among female college students of Karachi, Pakistan. METHODS: A cross-sectional study was conducted on 1000 students from June to December 2016. Data were collected using a pre-piloted questionnaire, and SPSS (version 20) was used for data analysis. Chi-square test was used to determine the significance of the difference in knowledge and attitude among participants who had performed BSE and those who had not. A P < 0.05 was considered statistically significant. RESULTS: The overall response rate was 90.9%. Although 71.4% of the women knew what BSE was, only 33.1% had performed it. Majority of the participants considered that BSE is important in the early detection of breast cancer. With 59.7% of the population having an adequate amount of knowledge, 87.2% had a positive attitude toward BSE. A medical background was found to be a significant predictor for adequate knowledge and a positive attitude toward BSE. CONCLUSION: Despite a positive attitude found among the population toward BSE, its knowledge and practice were inadequate. Educational interventions are required in Pakistan to encourage young women to perform BSE regularly so that breast abnormalities can be detected early on and mortalities can be minimized.
Selection of reference genes for quantitative real‐time polymerase chain reaction studies in rat osteoblasts

Quantitative real‐time polymerase chain reaction (qRT‐PCR) is a powerful tool to evaluate gene expression, but its accuracy depends on the choice and stability of the reference genes used for normalization. In this study, we aimed to identify reference genes for studies on osteoblasts derived from rat bone marrow mesenchymal stem cells (bone marrow osteoblasts), osteoblasts derived from newborn rat calvarial (calvarial osteoblasts), and rat osteosarcoma cell line UMR‐106. The osteoblast phenotype was characterized by ALP activity and extracellular matrix mineralization. Thirty‐one candidates for reference genes from a Taqman® array were assessed by qRT‐PCR, and their expressions were analyzed by five different approaches. The data showed that several of the most traditional reference genes, such as Actb and Gapdh, were inadequate for normalization and that the experimental conditions may affect gene stability. Eif2b1 was frequently identified among the best reference genes in bone marrow osteoblasts, calvarial osteoblasts, and UMR‐106 osteoblasts. Selected stable and unstable reference genes were used to normalize the gene expression of Runx2, Alp, and Oc. The data showed statistically significant differences in the expression of these genes depending on the stability of the reference gene used for normalization, creating a bias that may induce incorrect assumptions in terms of osteoblast characterization of these cells. In conclusion, our study indicates that a rigorous selection of reference genes is a key step in qRT‐PCR studies in osteoblasts to generate precise and reliable data.
Association study of gut flora in Wilson's disease through high-throughput sequencing

Abstract In this study, we analyzed the difference of intestinal flora polymorphisms between Wilson's disease (WD) patients and healthy people by high-throughput sequencing technology, and explored the correlation between WD and intestinal flora polymorphism. A total of 22 cases of WD patients and 22 healthy persons as control were recruited. The total DNA was extracted from the fecal specimens of all the subjects, V4 high variable region of 16S rRNA gene was amplified and sequenced by high-throughput sequencing. The sequencing results were analyzed by &agr; diversity and &bgr; diversity. The unweighted UniFrac distance matrices were calculated and trees were built by unweighted-pair group method with arithmetic mean (UPGMA). A total of 2,548,262 sequences were obtained after the data are optimized, the average sequences in the WD group was 36,836 ± 4104 and it was 35,051 ± 3075 in the normal control group, there was no significant difference in the average sequence number between the 2 groups. OTU analysis showed that 2663 OTU were obtained in WD group, and 3271 OTU were obtained in the control group, of which 941 were common OTU. Colony diversity analysis showed that the intestinal flora of WD group and control group belonged to 5 phyla, they were Bacteroidetes, Firmicutes, Proteobacteria, Fusobacteria, and Tenericutes, respectively. In WD group, the abundance of Bacteroidetes was significantly lower than that of the control group (67.19% vs 76.75%, P < .001), and the abundance of Firmicutes (26.18% vs 19.83%, P < .001), Proteobacteria (4.31% vs 3.09%, P < .05), Fusobacteria (1.88% vs 0.04%, P < .001) were significantly higher than that of control group. Compared with the control group at the level of the genus, the abundance of Bacteroides (4.85% vs 4.6%, P < .05), Faecalibacterium (2.92% vs 2.13%, P < .05), Megamonas (0.84% vs 0.22%, P < .001), Lachnospira (0.16% vs 0.09%, P < .001) significantly increased in WD group, while the abundance of Prevotella (1.63% vs 2.48%, P < .001), Roseburia (0.75% vs 1.39%, P < .001) and Phascolarctobacterium (1.72% vs 2.45%, P < .001) significantly decreased in WD group. PCoA and UPGMA tree analysis showed that there were significant differences of gut microbial compositions between the 2 groups. The diversity and composition of intestinal flora in the WD patients were significantly lower than those in the healthy controls, and the diversity of intestinal flora may be associated with the presence of WD.
Key Extraction using Thermal Laser Stimulation: A Case Study on Xilinx Ultrascale FPGAs

Thermal laser stimulation (TLS) is a failure analysis technique, which can be deployed by an adversary to localize and read out stored secrets in the SRAM of a chip. To this date, a few proof-of-concept experiments based on TLS or similar approaches have been reported in the literature, which do not reflect a real attack scenario. Therefore, it is still questionable whether this attack technique is applicable to modern ICs equipped with side-channel countermeasures. The primary aim of this work is to assess the feasibility of launching a TLS attack against a device with robust security features. To this end, we select a modern FPGA, and more specifically, its key memory, the so-called battery-backed SRAM (BBRAM), as a target. We demonstrate that an attacker is able to extract the stored 256-bit AES key used for the decryption of the FPGA’s bitstream, by conducting just a single non-invasive measurement. Moreover, it becomes evident that conventional countermeasures are incapable of preventing our attack since the FPGA is turned off during key recovery. Based on our time measurements, the required effort to develop the attack is shown to be less than 7 hours. To avert this powerful attack, we propose a low-cost and CMOS compatible countermeasure circuit, which is capable of protecting the BBRAM from TLS attempts even when the FPGA is powered off. Using a proof-of-concept prototype of our countermeasure, we demonstrate its effectiveness against TLS key extraction attempts.
The analgesic effects of intraoperative total intravenous anesthesia (TIVA) with propofol versus sevoflurane after colorectal surgery

Abstract Clinical studies have shown that total intravenous anesthesia (TIVA) with propofol is associated with better postoperative pain control compared with inhalational anesthesia, while other studies have not shown any benefit. The analgesic effect of TIVA with propofol in colorectal surgery has not been studied. The aim of this study is to evaluate the postoperative analgesic effects of TIVA with propofol versus inhalational sevoflurane in colorectal surgery. This is a retrospective case–control study. Records of patients undergoing colorectal surgery from 2014 to 2016 (36 months) were retrieved. Ninety-five patients who received TIVA with propofol were matched against 95 patients who received inhalational sevoflurane. Acute postoperative numerical rating scale (NRS) pain scores, postoperative morphine consumption, patient satisfaction, and side effects were compared and analyzed for differences between TIVA with propofol and sevoflurane. There were no significant differences in NRS pain scores, incidence of side effects, and patient satisfaction between the 2 groups. Patients receiving TIVA with propofol had significantly reduced total morphine consumption (P < .001), and daily morphine consumption on postoperative days 1 (P = .031), 2 (P = .002), and 3 (P = .031) compared with those receiving sevoflurane. TIVA with propofol was not associated with improved postoperative analgesia, better patient satisfaction, or reduced side effects. It may reduce postoperative opioid consumption after colorectal surgery.
The relationship between seminal plasma and serum trace elements and semen parameters of dromedary camels (Camelus dromedarius)

A total of 14 camels of known fertility (controls) at the Camel Research Centre, King Faisal University and 41 infertile dromedaries brought to the Veterinary Teaching Hospital were used in this study during the breeding season. Seminal plasma and serum were obtained from all males, and stored at -80°C until analysis. Concentrations of six trace elements (manganese [Mn], zinc [Zn], iron [Fe], selenium [Se], copper [Cu] and chromium [Cr]) were estimated in the seminal plasma and serum using an atomic absorption spectrophotometer. Results showed significant (p < 0.05 and p < 0.01) differences in semen pH and sperm motility, concentration and abnormalities between the control and infertile dromedaries. In seminal plasma, a significant (p < 0.01) difference in Fe concentrations (7.792 ± 1.567 vs. 61.259 ± 11.967 mg/L) and significant (p < 0.05) differences in Zn (24.763 ± 8.206 vs. 83.981 ± 4.972 mg/L) and Cu (1.653 ± 0.348 vs. 7.905 ± 1.134 mg/L) concentrations existed between the control and infertile dromedaries, respectively. In serum, there were significant (p < 0.05) differences in Fe (6.237 ± 0.695 vs. 151.121 ± 27.604 mg/L) and Cu (24.703 ± 4.195 vs. 6.375 ± 0.644 mg/L) concentrations and a significant (p < 0.01) difference in Zn concentrations (17.086 ± 1.606 vs. 90.422 ± 4.347 mg/L) between the fertile and infertile camels, respectively. There were significant (p < 0.05) positive (r = 0.51) and negative (r = -0.54) correlations between Zn concentrations in seminal plasma and both sperm motility and sperm abnormalities, respectively. In conclusion, seminal plasma and serum trace elements of Fe, Zn and Cu influenced the semen parameters and fertility of dromedary camels. Low concentrations of Fe, Zn and Cu in the seminal plasma were associated with normal fertility in dromedaries.