Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification and nucleotide sequence of Rhizobium meliloti insertion sequence ISRm3: similarity between the putative transposase encoded by ISRm3 and those encoded by Staphylococcus aureus IS256 and Thiobacillus ferrooxidans IST2

The insertion sequence ISRm3 was discovered simultaneously in different Rhizobium meliloti strains by probing Southern blots of total cellular DNA with 32P-labeled pTA2. This plasmid is indigenous to strain IZ450 and fortuitously contained four copies of ISRm3. By using an internal EcoRI fragment as a specific probe (pRWRm31), homology to ISRm3 was subsequently detected in over 90% of R. meliloti strains tested from different geographical locations around the world. The frequency of stable nonlethal ISRm3 transpositions was estimated to be 4 x 10(-5) per generation per cell in strain SU47 when grown in liquid culture. The entire nucleotide sequence of ISRm3 in R. meliloti 102F70 is 1,298 bp and has 30-bp terminal inverted repeats which are perfectly matched. Analysis of six copies of ISRm3 in two strains showed that a variable number of base pairs (usually eight or nine) were duplicated and formed direct repeats adjacent to the site of insertion. On one DNA strand, ISRm3 contains an open reading frame spanning 93% of its length. Comparison of the putative protein encoded with sequences derived from the EMBL and GenBank databases showed significant similarity between the putative transposases of ISRm3 from R. meliloti, IS256 from Staphylococcus aureus, and IST2 from Thiobacillus ferroxidans. These insertion sequences appear to be distantly related members of a distinct class.
A powerful association test of multiple genetic variants using a random‐effects model

There is an emerging interest in sequencing‐based association studies of multiple rare variants. Most association tests suggested in the literature involve collapsing rare variants with or without weighting. Recently, a variance‐component score test [sequence kernel association test (SKAT)] was proposed to address the limitations of collapsing method. Although SKAT was shown to outperform most of the alternative tests, its applications and power might be restricted and influenced by missing genotypes. In this paper, we suggest a new method based on testing whether the fraction of causal variants in a region is zero. The new association test, T REM, is derived from a random‐effects model and allows for missing genotypes, and the choice of weighting function is not required when common and rare variants are analyzed simultaneously. We performed simulations to study the type I error rates and power of four competing tests under various conditions on the sample size, genotype missing rate, variant frequency, effect directionality, and the number of non‐causal rare variant and/or causal common variant. The simulation results showed that T REM was a valid test and less sensitive to the inclusion of non‐causal rare variants and/or low effect common variants or to the presence of missing genotypes. When the effects were more consistent in the same direction, T REM also had better power performance. Finally, an application to the Shanghai Breast Cancer Study showed that rare causal variants at the FGFR2 gene were detected by T REM and SKAT, but T REM produced more consistent results for different sets of rare and common variants. Copyright © 2013 John Wiley & Sons, Ltd.
Measuring E-Commerce Success in Malaysia: Modified Delone Mclean Model with Trust and Privacy

With the influx of the Internet, Business to Customer E-commerce (B2c e-Commerce) has gained popularity around the globe. Although a number of researches has been done to know the factors affecting the adoption of e-commerce, however, not much research has done to know the effects of e-commerce on online shopping success. DeLone and McLean introduced the Information System (IS) success model in year 1992, later on, refined the same model with the newer concept, to fully understand the effects of IS success. In this paper, authors have used the DeLone and McLean IS success model 2003 with addition two constructs Privacy and Trust. Seven interrelated dimensions of e-commerce were examined namely, Service Quality, System Quality, Trust, Privacy, User satisfaction and Net benefits. The proposed model and the relationship between the various constructs were measured by gathering the data from 381 users from the business faculty of Klang valley universities in Malaysia. Seven interconnected dimensions of B2C e-commerce success were confirmed namely, Service Quality, System Quality, Information Quality, Trust, Privacy along with User Satisfaction and Net Benefits. Confirmatory Factor Analysis CFA and SEM were used to analyse the collected data and proved the hypothesis. The results revealed that system quality, service quality, trust and privacy are directly related to user satisfaction which in turn directly related to net benefits, whereas information quality has no any influence on user satisfaction in terms of e-commerce.
Familial and ethnic risk in inflammatory bowel disease

Familial aggregation in inflammatory bowel disease (IBD) has been established for several decades, reflecting shared genetic and environmental susceptibility. A positive family history remains the strongest recognizable risk factor for the development of IBD and is reported in around 8-12% of IBD patients. Crohn’s disease shows a more frequent familial pattern than ulcerative colitis. The risk of developing IBD in first-degree relatives of an affected proband is increased 4- to 8-fold. The risk for twins and children born from couples who both have IBD is also substantially higher; a cumulative effect of the number of family members affected has been described, with the highest incidence being described for families with three or more affected members. Herein, we review the available evidence regarding familial IBD, and briefly discuss the variation of IBD across different races and ethnicities, hoping to provide a useful update and a practical guide that can serve clinicians as a guide for counseling.
Intravenous Thrombolysis for Acute Ischaemic Stroke: Preliminary Experience with Recombinant Tissue Plasminogen Activator in the UK

Objective: To present the preliminary experience of implementing intravenous thrombolytic therapy for acute ischaemic stroke in three UK stroke centres. Background: Recombinant tissue plasminogen activator for ischaemic stroke received approval from UK regulatory authorities in April 2003. Since 1997, a small number of UK centres had used thrombolytic therapy in highly selected stroke patients. We present the early experience of that treatment in Glasgow and Newcastle. Design: Patients were selected and treated in accordance with the American Heart Association guidelines. Additionally, radiologic criteria employed in the European-Australasian Acute Stroke Studies were applied.National Institutes of Health Stroke Scale (NIHSS) scores were measured on admission, and Modified Rankin Scale (MRS) scores were assessed at 3 months for all patients with stroke treated prior to initiation of the Safe Implementation of Thrombolysis in Stroke monitoring program for implementation of thrombolysis in stroke in April 2001. Intracranial and systemic haemorrhagic complications were recorded. Results: 120 patients received thrombolytic treatment (approximately 1% of all admissions with presumed stroke). Mean age was 69 years (range 22–93) and initial median NIHSS score was 17 (range 3–31). In the two centres for which temporal data were available, the mean delay between symptom onset and treatment was 139 min (range 20–185). Sixteen episodes of cerebral haemorrhage or haemorrhagic transformation of any degree occurred, of which 5 (4%) were symptomatic. One patient deteriorated and died before repeat CT imaging could be performed. One non-fatal episode of systemic bleeding occurred. One patient was lost to follow-up. At 3 months, 31% of recipients had achieved good (MRS 0–1) outcome, 22% moderate (MRS 2–3) outcome and 21% (MRS 4–5) poor outcome. Twenty-one per cent died within 3 months of stroke. Observed frequency of bleeding complications and protocol violations (6%) was similar to those reported elsewhere. Conclusion: A small proportion of stroke patients received thrombolytic treatment. Patients treated were more severely affected than in other published European and North American series. Outcomes and complications were consistent with experience elsewhere.
How Salinity Damages Citrus: Osmotic Effects and Specific Ion Toxicities

ADDITIONAL INDEX WORDS. sodium, chloride, boron SUMMARY. There are two ways salinity can damage citrus: direct injury due to specifi c ions, and osmotic effects. Specifi c ion toxicities are due to accu- mulation of sodium, chloride, and/or boron in the tissue to damaging levels. The damage is visible as foliar chlorosis and necrosis and, if severe enough, will affect orchard productiv- ity. These ion accumulations occur in two ways. The fi rst, more controllable and less frequent method, is direct foliar uptake. Avoiding irrigation methods that wet the foliage can eas- ily eliminate this form of specifi c ion damage. The second way specifi c ion toxicity can occur is via root uptake. Certain varieties or rootstocks are better able to exclude the uptake and translocation of these potentially damaging ions to the shoot and are more tolerant of salinity. The effect of specifi c ions, singly and in combina- tion, on plant nutrient status can also be considered a specifi c ion effect. The second way salinity damages citrus is osmotic effects. Osmotic effects are caused not by specifi c ions but by the total concentration of salt in the soil solution produced by the combina- tion of soil salinity, irrigation water quality, and fertilization. Most plants have a threshold concentration value above which yields decline. The arid climates that produce high quality fresh citrus fruit are also the climates that exacerbate the salt concentra- tion in soil solution that produces the osmotic effects. Osmotic effects can be slow, subtle, and often indis- tinguishable from water stress. With the exception of periodic leaching, it 1
ANALYSIS OF THE EFFECT OF BLOCK OWNERSHIP ON PERFORMANCE OF LISTED COMMERCIAL BANKS IN NAIROBI COUNTY, KENYA

This study examined the effect of block ownership on organisational performance of listed commercial banks in Kenya. The study used descriptive cross-sectional survey. The study targeted 11 commercial banks in Kenya which are listed in the NSE. The total population that was being studied was a total of 283 staff at managerial level employed at headquarters of commercial banks in Kenya. The unit of observation was 164 respondents selected using Fisher, Laing and Stoeckel formula. Each member of the selected sample was issued with a questionnaire by the researcher . Descriptive statistics were used in analysing quantitative data by use of SPSS and presentations were done using standard deviations , percentages, frequencies and frequencies means. Qualitative data was tested using content analysis. Correlation analysis was conducted to establish the degree of association between the predictor and the response variables . Multiple regressions were then done to establish the influence of block ownership on organisational performance of listed commercial banks in Kenya. The study established that “block ownership’, had significant effect on organisational performance of listed commercial banks in Kenya. The study concluded that there was statistically significant relationship between block ownership and organisational performance. Therefore it is important that organizations incorporate block ownership in their firms since block holders are essential in ensuring so as to safeguard the organizational performance. Key words: Block Ownership and Performance of Banks CITATION: Mirugi, T. , & Kinyua, G. M. (2018). Analysis of the effect of block ownership on performance of listed commercial banks in Nairobi County, Kenya. The Strategic Journal of Business & Change Management, 5(4), 2180 - 2188.
Association with TP53 codon 72 polymorphism and the risk of non‐Hodgkin lymphoma

Polymorphism of TP53 Arg72Pro is associated with many different cancers[1-4]. Few studies have investigated its role in the susceptibility to non-Hodgkin lymphoma (NHL) [5,6]. To examine the association between this polymorphism and NHL risk, we conducted a Korean large-scale, population-based case-control study (945 cases and 1,700 controls). The TP53 72CC genotype was associated with increased risk of NHL (P 5 0.04) and diffuse large B-cell lymphoma (P 5 0.04). Our findings provide evidence that the TP53 Arg72Pro is associated with an increased risk of NHL in Korea.
Epistasis and the Adaptability of an RNA Virus

We have explored the patterns of fitness recovery in the vesicular stomatitis RNA virus. We show that, in our experimental setting, reversions to the wild-type genotype were rare and fitness recovery was at least partially driven by compensatory mutations. We compared compensatory adaptation for genotypes carrying (1) mutations with varying deleterious fitness effects, (2) one or two deleterious mutations, and (3) pairs of mutations showing differences in the strength and sign of epistasis. In all cases, we found that the rate of fitness recovery and the proportion of reversions were positively affected by population size. Additionally, we observed that mutations with large fitness effect were always compensated faster than mutations with small fitness effect. Similarly, compensatory evolution was faster for genotypes carrying a single deleterious mutation than for those carrying pairs of mutations. Finally, for genotypes carrying two deleterious mutations, we found evidence of a negative correlation between the epistastic effect and the rate of compensatory evolution.
Analysis of urinary human chorionic gonadotrophin concentrations in normal and failing pregnancies using longitudinal, Cox proportional hazards and two-stage modelling

Background Human chorionic gonadotrophin is a marker of early pregnancy. This study sought to determine the possibility of being able to distinguish between healthy and failing pregnancies by utilizing patient-associated risk factors and daily urinary human chorionic gonadotrophin concentrations. Methods Data were from a study that collected daily early morning urine samples from women trying to conceive (n = 1505); 250 of whom became pregnant. Data from 129 women who became pregnant (including 44 miscarriages) were included in these analyses. A longitudinal model was used to profile human chorionic gonadotrophin, a Cox proportional hazards model to assess demographic/menstrual history data on the time to failed pregnancy, and a two-stage model to combine these two models. Results The profile for log human chorionic gonadotrophin concentrations in women suffering miscarriage differs to that of viable pregnancies; rate of human chorionic gonadotrophin rise is slower in those suffering a biochemical loss (loss before six weeks, recognized by a rise and fall of human chorionic gonadotrophin) and tends to plateau at a lower log human chorionic gonadotrophin in women suffering an early miscarriage (loss six weeks or later), compared with viable pregnancies. Maternal age, longest cycle length and time from luteinizing hormone surge to human chorionic gonadotrophin reaching 25 mIU/mL were found to be significantly associated with miscarriage risk. The two-stage model found that for an increase of one day in the time from luteinizing hormone surge to human chorionic gonadotrophin reaching 25 mIU/mL, there is a 30% increase in miscarriage risk (hazard ratio: 1.30; 95% confidence interval: 1.04, 1.62). Conclusion Rise of human chorionic gonadotrophin in early pregnancy could be useful to predict pregnancy viability. Daily tracking of urinary human chorionic gonadotrophin may enable early identification of some pregnancies at risk of miscarriage.
Evaluation of Modified Patient Health Questionnaire-9 Teen in South African Adolescents

Background: This report describes the findings of the survey conducted by South African Depression and Anxiety Group (SADAG) in a cohort of disadvantaged South African adolescent scholars in which PHQ-9 teen version was modified and adapted (PHQ-9M) to improve the response rate. Method: PHQ-9M was administered to 2025 secondary students of 35 schools over 2 years in Gauteng Province, South Africa. Results: The mean age was 15.8 years. 23% of respondents scored 9 or above, 26.7% reported suicidal ideations in last month, 21.8% reported lifetime suicide attempts and 9.3% had severe functional impairment. PHQ-9M showed a fair internal consistency and face validity in this study. Conclusion: The PHQ-9M could be useful in conducting surveys in settings of low mental health literacy after further evaluation of its psychometric properties. The high rates of suicidal ideations, suicide attempts, and functional impairment in this population suggest the need for preventive interventions.
Class Action Spillover Effects on Joint Venture Partners

Firms exhibit a US$106MM average market capitalization decline when a lawsuit against their joint venture partner is announced. Following their partner’s lawsuit, the probability of facing similar litigation increases for other firms in the venture, and such legal action is 2.3 times more likely whenever all partners share a common institutional owner. After their partner’s lawsuit, the non-sued venture firms increase cash reserves but decrease dividends, investment, acquisition spending, and disclosure quality. Moreover, their volatility of stock returns, cash flows, and assets also decline. Although these venture firms are not named in their partner’s complaint, they suffer value-reducing spillover effects
Hotel Manager Strategies to Reduce Voluntary Employee Turnover

Hotel Manager Strategies to Reduce Voluntary Employee Turnover by Christl A. Kroeten MS, University of Nebraska, Lincoln, 2011 BA, University of Nebraska, Lincoln, 2005 Doctoral Study Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Business Administration Walden University July 2018 Abstract The voluntary turnover rate in the United States hotel industry is among the highest of all industries, resulting in lost revenue. The purpose of this multiple case study was to explore strategies hotel managers use to reduce voluntary employee turnover. The targeted population consisted of 6 managers from hotel businesses operating in the MidAtlantic region of the United States who successfully used strategies to reduceThe voluntary turnover rate in the United States hotel industry is among the highest of all industries, resulting in lost revenue. The purpose of this multiple case study was to explore strategies hotel managers use to reduce voluntary employee turnover. The targeted population consisted of 6 managers from hotel businesses operating in the MidAtlantic region of the United States who successfully used strategies to reduce voluntary employee turnover. Job embeddedness theory, this study’s theoretical framework, was used to describe reasons employees remained in organizations. Data were gathered via semistructured interviews, observational notes, and public business records regarding turnover or retention programs. Yinʼs 5-step analysis model was used to compile, deconstruct, reassemble, interpret, and draw conclusions from the data. Four themes emerged from data analysis: organizational support with property-level flexibility, feeling valued for individual contributions to the team, opportunities for training or advancement, and relationships with managers and peers. The results of this study may contribute to positive social change by providing strategies to reduce employee turnover in a historically low-wage industry, which may result in raising the quality of life for hotel employees, their families, and communities. Hotel Manager Strategies to Reduce Voluntary Employee Turnover by Christl A. Kroeten MS, University of Nebraska, Lincoln, 2011 BA, University of Nebraska, Lincoln, 2005 Doctoral Study Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Business Administration Walden University July 2018 Dedication I would like to dedicate this study to my family. To my parents for paving the way and setting the doctoral standard. To my husband, Robert Carey for his untiring support and creative motivation. Further, to my children Hunter and Morgan for keeping me grounded in real-life when I could have gotten lost in literature and business analysis. I cannot thank you enough. You continue to allow me to be the professional I truly want
Pharmacoeconomics and clinical practice guidelines. A survey of attitudes in Swedish formulary committees.

BACKGROUND Swedish formulary committees are expected to influence prescribing practice by establishing and issuing drug lists and clinical practice guidelines, particularly now that financial responsibility for prescription drugs has been transferred from the national to the county council level. OBJECTIVE The purpose of this exploratory study was to identify the information sources and decision criteria that individual committee members perceive as important in establishing clinical practice guidelines. Moreover, obstacles to the increased use of pharmacoeconomic evaluations in decision-making were also identified. DESIGN AND SETTING Data were gathered through a survey questionnaire administered in 1998 to members of central formulary committees throughout Sweden, as determined by a national register. PARTICIPANTS 312 members of central formulary committees, of whom 69% responded. RESULTS Treatment policies/guidelines supplied by government authorities, or found in reviewed journals, are considered the most important sources of information, and criteria associated with costs and effects are considered the most important decision criteria. The members' years of experience and their professions affect their assessments of information sources, whereas education in health economics affects their assessments of decision criteria. Committee members voiced an interest in pharmacoeconomic issues, but warned that there was neither sufficient competence among committee members nor an adequate supply of relevant studies. Furthermore, a majority of the members identified difficulty in translating study results into clinical practice guidelines and limited possibilities in comparing studies as obstacles to the increased use of pharmacoeconomic evaluations. CONCLUSIONS The results of this survey may be useful in designing future economic evaluations and when presenting and diffusing study results.
Repressive coping and blood lipids in men and women.

&NA; Studies have suggested that a repressive coping style, characterized by defensiveness against negative emotions, may be related to several adverse health outcomes. This study examined whether repressive coping is associated with blood lipids, and whether this association is influenced by age or sex. One hundred fourteen healthy adults completed the Marlowe‐Crowne scale (MC) and the Bendig version of the Taylor Manifest Anxiety scale (TMAS) prior to having their blood drawn after an overnight fast. Hierarchical regression analyses showed a significant interaction of sex, MC, and TMAS on total cholesterol (F(1, 104) = 4.41, p < 0.05), after controlling for the influence of age, body mass index, and other main effects and interactions. Results showed that male repressors (high MC; low TMAS) had the highest cholesterol levels, while truly low anxious males (low MC; low TMAS) had the lowest levels. The opposite pattern was noted for women. There were no interactive effects of age and coping style on lipids. The results suggest that males who repress negative emotions may be at greater risk for atherosclerotic diseases.