Miracle-dz/UniFAR-FTUs huggingface dataset:
Exine dehiscing induces rape icrospore polarity , which results in different daughter cell fate and fixes the apical – basal axis of the embryo

In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylation level and pattern were analysed in actively growing organs, under short(6 h) and long(2 d or 4 d) term and low (10 mM) and high (50 mM) doses of Cd, through a Methylation-Sensitive Amplification Polymorphism technique and an immunocytological approach, respectively. The expression of one member of the CHROMOMETHYLASE (CMT) family, a DNA methyltransferase, was also assessed by qRT-PCR. Nuclear chromatin ultrastructure was investigated by transmission electron microscopy. Cd treatment induced a DNA hypermethylation, as well as an up-regulation of CMT, indicating that de novo methylation did indeed occur. Moreover, a high dose of Cd led to a progressive heterochromatinization of interphase nuclei and apoptotic figures were also observed after long-term treatment. The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase. Such changes are linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin. Overall, the data show an epigenetic basis to the mechanism underlying Cd toxicity in plants.
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report

Background Neutralizing antibody assessments play a central role in human immunodeficiency virus type-1 (HIV-1) vaccine development but it is unclear which assay, or combination of assays, will provide reliable measures of correlates of protection. To address this, an international collaboration (NeutNet) involving 18 independent participants was organized to compare different assays. Methods Each laboratory evaluated four neutralizing reagents (TriMab, 447-52D, 4E10, sCD4) at a given range of concentrations against a panel of 11 viruses representing a wide range of genetic subtypes and phenotypes. A total of 16 different assays were compared. The assays utilized either uncloned virus produced in peripheral blood mononuclear cells (PBMCs) (virus infectivity assays, VI assays), or their Env-pseudotyped (gp160) derivatives produced in 293T cells (PSV assays) from molecular clones or uncloned virus. Target cells included PBMC and genetically-engineered cell lines in either a single- or multiple-cycle infection format. Infection was quantified by using a range of assay read-outs that included extracellular or intracellular p24 antigen detection, RNA quantification and luciferase and beta-galactosidase reporter gene expression. Findings PSV assays were generally more sensitive than VI assays, but there were important differences according to the virus and inhibitor used. For example, for TriMab, the mean IC50 was always lower in PSV than in VI assays. However, with 4E10 or sCD4 some viruses were neutralized with a lower IC50 in VI assays than in the PSV assays. Inter-laboratory concordance was slightly better for PSV than for VI assays with some viruses, but for other viruses agreement between laboratories was limited and depended on both the virus and the neutralizing reagent. Conclusions The NeutNet project demonstrated clear differences in assay sensitivity that were dependent on both the neutralizing reagent and the virus. No single assay was capable of detecting the entire spectrum of neutralizing activities. Since it is not known which in vitro assay correlates with in vivo protection, a range of neutralization assays is recommended for vaccine evaluation.
Improving Public Transit Accessibility for Blind Riders by Crowdsourcing Bus Stop Landmark Locations with Google Street View: An Extended Analysis

Low-vision and blind bus riders often rely on known physical landmarks to help locate and verify bus stop locations (e.g., by searching for an expected shelter, bench, or newspaper bin). However, there are currently few, if any, methods to determine this information a priori via computational tools or services. In this article, we introduce and evaluate a new scalable method for collecting bus stop location and landmark descriptions by combining online crowdsourcing and Google Street View (GSV). We conduct and report on three studies: (i) a formative interview study of 18 people with visual impairments to inform the design of our crowdsourcing tool, (ii) a comparative study examining differences between physical bus stop audit data and audits conducted virtually with GSV, and (iii) an online study of 153 crowd workers on Amazon Mechanical Turk to examine the feasibility of crowdsourcing bus stop audits using our custom tool with GSV. Our findings reemphasize the importance of landmarks in nonvisual navigation, demonstrate that GSV is a viable bus stop audit dataset, and show that minimally trained crowd workers can find and identify bus stop landmarks with 82.5% accuracy across 150 bus stop locations (87.3% with simple quality control).
District Leadership that Works: Striking the Right Balance (review)

Robert J. Marzano and Timothy Waters, co-authors of District Leadership that Works: Striking the Right Balance, are world-renowned authors, researchers, and leaders in education with sixty-three years combined in public education. Marzano is also co-founder and CEO of Marzano Research Laboratory in Centennial, Colorado, and senior scholar at Mid-continent Research for Education and Learning (McREL) in Denver. Waters, in addition to serving as president and CEO for McREL since 1993, spent twenty-three years in the K–12 system as a superintendent, assistant superintendent, high school principal, assistant principal, and teacher. Both men are leaders in research and the applied practice of that research into programs and tools for K–12 teachers and administrators. The purpose of District Leadership that Works is to establish the fact that there is a direct relationship between district leadership and student achievement. Marzano and Waters conducted a study to answer two research questions, the results of which are the contents of the book. The book’s primary audience is school district administrators, but K–12 teachers would find the study enlightening as well, with insight and ideas that can apply to individual classrooms, if not a district-wide initiative. Many educators may already be familiar with Marzano’s previous works, as he is a leader in educational research and instruction.
Smoking, Suicidality and Psychosis: A Systematic Meta-Analysis

The aim of this study is to systematically review the literature that explored the association between smoking and suicidal risk among those with serious mental illness and to estimate the risk of suicidal behaviors attributable to smoking among this patient group. Multiple databases (CINAHL, PsycINFO, EMBASE, Informit Health Collection and the Cochrane Library databases) were searched from 1 January 1975 through 15 January 2014, along with references from relevant articles for observational studies that ascertained the association between smoking and suicidal behaviors among patients with psychotic disorders conducted in adult patients. Thirteen studies involving 6813 patients with severe mental illness were included. We found that smoking was significantly associated with suicidality in psychosis with an Odds Ratio of 2.12 (95% CI 1.67–2.7). Smoking is associated with suicidal risk amongst individuals with a severe mental illness; however, it is still unclear whether this represents a true risk factor or a confounder or a mediator via mechanisms, hitherto unknown, needs to be studied further.
Genetic structure of Argentinean hexaploid wheat germplasm

The identification of genetically homogeneous groups of individuals is an ancient issue in population genetics and in the case of crops like wheat, it can be valuable information for breeding programs, genetic mapping and germplasm resources. In this work we determined the genetic structure of a set of 102 Argentinean bread wheat (Triticum aestivum L.) elite cultivars using 38 biochemical and molecular markers (functional, closely linked to genes and neutral ones) distributed throughout 18 wheat chromosomes. Genetic relationships among these lines were examined using model-based clustering methods. In the analysis three subpopulations were identified which correspond largely to the origin of the germplasm used by the main breeding programs in Argentina.
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning

In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylation level and pattern were analysed in actively growing organs, under short(6 h) and long(2 d or 4 d) term and low (10 mM) and high (50 mM) doses of Cd, through a Methylation-Sensitive Amplification Polymorphism technique and an immunocytological approach, respectively. The expression of one member of the CHROMOMETHYLASE (CMT) family, a DNA methyltransferase, was also assessed by qRT-PCR. Nuclear chromatin ultrastructure was investigated by transmission electron microscopy. Cd treatment induced a DNA hypermethylation, as well as an up-regulation of CMT, indicating that de novo methylation did indeed occur. Moreover, a high dose of Cd led to a progressive heterochromatinization of interphase nuclei and apoptotic figures were also observed after long-term treatment. The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase. Such changes are linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin. Overall, the data show an epigenetic basis to the mechanism underlying Cd toxicity in plants.
ESC Guidelines on diabetes, pre-diabetes, and cardiovascular diseases developed in collaboration with the EASD

Authors/Task Force Members: Lars Rydén* (ESC Chairperson) (Sweden), Peter J. Grant* (EASD Chairperson) (UK), Stefan D. Anker (Germany), Christian Berne (Sweden), Francesco Cosentino (Italy), Nicolas Danchin (France), Christi Deaton (UK), Javier Escaned (Spain), Hans-Peter Hammes (Germany), Heikki Huikuri (Finland), Michel Marre (France), Nikolaus Marx (Germany), Linda Mellbin (Sweden), Jan Ostergren (Sweden), Carlo Patrono (Italy), Petar Seferovic (Serbia), Miguel Sousa Uva (Portugal), Marja-Riita Taskinen (Finland), Michal Tendera (Poland), Jaakko Tuomilehto (Finland), Paul Valensi (France), Jose Luis Zamorano (Spain)
Performance of pulsed OFDM systems for UWB communication

This A multi-band orthogonal frequency division multiplexing (OFDM) UWB system is the new proposal for the IEEE 802.15. 3a standard for wireless high bit-rate personal area networks. Pulsed OFDM is an enhancement to the multi-band OFDM system that offers better performance with lower complexity and correspondingly reduced power consumption. In this paper, the implementation issues and pipeline structures of a pulsed OFDM transmitter are presented. In particular, architectural features of the system and its components are described and analyzed.
Beamforming with limited feedback in amplify-and-forward cooperative networks - [transactions letters]

A relay selection approach has previously been shown to outperform repetition-based scheduling for both amplify-and-forward (AF) and decode-and-forward (DF) cooperative networks. The selection method generally requires some feedback from the destination to the relays and the source, raising the issue of the interplay between performance and feedback rate. In this letter, we treat selection as an instance of limited feedback distributed beamforming in cooperative AF networks, and highlight the differences between transmit beamforming in a traditional multi-input single-output (MISO) system and the distributed case. Specifically, Grassmannian line packing (GLP) is no longer the optimal codebook design, and orthogonal codebooks are no longer equivalent to each other. We derive the high signal-to-noise ratio expressions for outage probability and probability of symbol error for unlimited-feedback and selection schemes, which are then used for performance comparisons. The selection protocol is compared to a limited-feedback distributed beamformer that assigns codebooks based on the Generalized Lloyd algorithm (GLA), and one that uses random beam-vectors. The main conclusion is that the performance improvement to be seen using the very complex GLA is small, and that many more feedback bits are required with random beamforming than selection for the same performance. These results indicate that the selection protocol is a very attractive protocol, with low complexity, that provides excellent performance relative to other known methods.
Child–Computer Interaction at the Beginner Stage of Music Learning: Effects of Reflexive Interaction on Children’s Musical Improvisation

In this article children’s musical improvisation is investigated through the “reflexive interaction” paradigm. We used a particular system, the MIROR-Impro, implemented in the framework of the MIROR project (EC-FP7), which is able to reply to the child playing a keyboard by a “reflexive” output, mirroring (with repetitions and variations) her/his inputs. The study was conducted in a public primary school, with 47 children, aged 6–7. The experimental design used the convergence procedure, based on three sample groups allowing us to verify if the reflexive interaction using the MIROR-Impro is necessary and/or sufficient to improve the children’s abilities to improvise. The following conditions were used as independent variables: to play only the keyboard, the keyboard with the MIROR-Impro but with not-reflexive reply, the keyboard with the MIROR-Impro with reflexive reply. As dependent variables we estimated the children’s ability to improvise in solos, and in duets. Each child carried out a training program consisting of 5 weekly individual 12 min sessions. The control group played the complete package of independent variables; Experimental Group 1 played the keyboard and the keyboard with the MIROR-Impro with not-reflexive reply; Experimental Group 2 played only the keyboard with the reflexive system. One week after, the children were asked to improvise a musical piece on the keyboard alone (Solo task), and in pairs with a friend (Duet task). Three independent judges assessed the Solo and the Duet tasks by means of a grid based on the TAI-Test for Ability to Improvise rating scale. The EG2, which trained only with the reflexive system, reached the highest average results and the difference with EG1, which did not used the reflexive system, is statistically significant when the children improvise in a duet. The results indicate that in the sample of participants the reflexive interaction alone could be sufficient to increase the improvisational skills, and necessary when they improvise in duets. However, these results are in general not statistically significant. The correlation between Reflexive Interaction and the ability to improvise is statistically significant. The results are discussed on the light of the recent literature in neuroscience and music education.
Genetic Resources, Genome Mapping and Evolutionary Genomics of the Pig (Sus scrofa)

The pig, a representative of the artiodactyla clade, is one of the first animals domesticated, and has become an important agriculture animal as one of the major human nutritional sources of animal based protein. The pig is also a valuable biomedical model organism for human health. The pig's importance to human health and nutrition is reflected in the decision to sequence its genome (3X). As an animal species with its wild ancestors present in the world, the pig provides a unique opportunity for tracing mammalian evolutionary history and defining signatures of selection resulting from both domestication and natural selection. Completion of the pig genome sequencing project will have significant impacts on both agriculture and human health. Following the pig whole genome sequence drafts, along with large-scale polymorphism data, it will be possible to conduct genome sweeps using association mapping, and identify signatures of selection. Here, we provide a description of the pig genome sequencing project and perspectives on utilizing genomic technologies to exploit pig genome evolution and the molecular basis for phenotypic traits for improving pig production and health.
Accepted for Publication in Ieee Transactions on Signal Processing 1 Robust Transceiver Optimization in Downlink Multiuser Mimo Systems

—We study robust transceiver optimization in a down-link, multiuser, wireless system, where the transmitter and the receivers are equipped with antenna arrays. The robustness is defined with respect to imperfect knowledge of the channel at the transmitter. The errors in the channel state information are assumed to be bounded, and certain quality-of-service targets in terms of mean-square-errors (MSEs) are guaranteed for all channels from the uncertainty regions. Iterative algorithms are proposed for the transceiver design. The iterations perform alternating optimization of the transmitter and the receivers and have equivalent semidefinite programming representations with efficient numerical solutions. The framework supports robust counterparts of several MSE-optimization problems, including transmit power minimization with per-user or per-stream MSE constraints, sum MSE minimization, min-max fairness, etc. Although the convergence to the global optimum cannot be claimed due to the intricacy of the problems, numerical examples show good practical performance of the presented methods. We also provide various possibilities for extensions in order to accommodate a broader set of scenarios regarding the precoder structure, the uncertainty modeling, and a multi-cellular setup.
Coherent neural representation of hand speed in humans revealed by MEG imaging

The spiking activity of single neurons in the primate motor cortex is correlated with various limb movement parameters, including velocity. Recent findings obtained using local field potentials suggest that hand speed may also be encoded in the summed activity of neuronal populations. At this macroscopic level, the motor cortex has also been shown to display synchronized rhythmic activity modulated by motor behavior. Yet whether and how neural oscillations might be related to limb speed control is still poorly understood. Here, we applied magnetoencephalography (MEG) source imaging to the ongoing brain activity in subjects performing a continuous visuomotor (VM) task. We used coherence and phase synchronization to investigate the coupling between the estimated activity throughout the brain and the simultaneously recorded instantaneous hand speed. We found significant phase locking between slow (2- to 5-Hz) oscillatory activity in the contralateral primary motor cortex and time-varying hand speed. In addition, we report long-range task-related coupling between primary motor cortex and multiple brain regions in the same frequency band. The detected large-scale VM network spans several cortical and subcortical areas, including structures of the frontoparietal circuit and the cerebello–thalamo–cortical pathway. These findings suggest a role for slow coherent oscillations in mediating neural representations of hand kinematics in humans and provide further support for the putative role of long-range neural synchronization in large-scale VM integration. Our findings are discussed in the context of corticomotor communication, distributed motor encoding, and possible implications for brain–machine interfaces.
Knowing-Why About Data Processes and Data Quality

Knowledge about work processes is a prerequisite for performing work. We investigate whether a certain mode of knowledge, knowing-why, affects work performance and whether the knowledge held by different work roles matters for work performance. We operationalize these questions in the specific domain of data production processes and data quality. We analyze responses from three roles within data production processes, data collectors, data custodians, and data consumers, to investigate the effects of different knowledge modes held by different work roles on data quality. We find that work roles and the mode of knowledge do matter. Specifically, data collectors with why-knowledge about the data production process contribute to producing better quality data. Overall, knowledge of data collectors is more critical than that of data custodians.
Screening for visual impairment: Outcome among schoolchildren in a rural area of Delhi

Background: Uncorrected refractive errors are the main cause of vision impairment in school-aged children. The current study focuses on the effectiveness of school eye screening in correcting refractive errors. Objectives: 1. To study the magnitude of visual impairment among school children. 2. To assess the compliance of students for refraction testing, procurement and use of spectacles. Materials and Methods: An intervention study was conducted in schools of the north- west district of Delhi, in the rural field practice area of a medical college. Students studying in five government schools in the field practice area were chosen as the study subjects. Results: Out of 1123 students enrolled, 1075 (95.7%) students were screened for refractive errors. Low vision (visual acuity < 20/60) in the better eye was observed in 31 (2.9%) children and blindness (visual acuity <20/200) in 10 (0.9%) children. Compliance with referral for refraction was very low as only 51 (41.5%) out of 123 students could be tested for refraction. Out of 48 students, 34 (70.8%) procured spectacles from family resources but its regular use was found among only 10 (29.4%) students. The poor compliance among students stems out of various myths and perceptions regarding use of spectacles prevalent in the community. Conclusion: Refractive error is an important cause of avoidable blindness among rural school children. Behavior change communication among rural masses by spreading awareness about eye health and conducting operational research at school and community level to involve parent's teachers associations and senior students to motivate students for use of spectacles may improve utilization of existing eye health services in rural areas.
Comparative analyses of speech and language converge on birds

Abstract Unlike nonhuman primates, thousands of bird species have articulatory capabilities that equal or surpass those of humans, and they develop their vocalizations through vocal imitation in a way that is very similar to how human infants learn to speak. An understanding of how speech mechanisms have evolved is therefore unlikely to yield key insights into how the human brain is special.