Miracle-dz/UniFAR-FTUs huggingface dataset:
Isonatric Dialysis Biofeedback in Hemodiafiltration with Online Regeneration of Ultrafiltrate in Hypertensive Hemodialysis Patients: A Randomized Controlled Study

Dialysis biofeedback in hemodiafiltration with online regeneration of ultrafiltrate (HFR) could help to improve arterial hypertension. We evaluated the impact of isonatric HFR (HFR-iso) on hypertension control compared to conventional HFR. Forty-seven hemodialysis patients were included and randomized (ratio 2/1) HFR-iso versus HFR during 24 dialysis sessions. In the HFR-iso group (32 patients, 768 dialysis sessions), the predialytic systolic blood pressure (BP) decreased from S1 to S24 of 9 ± 20 mm Hg and increased of 5 ± 24 mm Hg in the HFR group (15 patients, 360 dialysis sessions), variation that differed between the 2 groups (ΔS1-S24, p = 0.035; interaction group*time, p = 0.012). The diastolic BP (HFR-iso -3 ± 14 mm Hg vs. HFR 5 ± 13 mm Hg; p = 0.088), the DDD of antihypertensive treatment and the dry weight did not vary significantly during the study. Number of sessions complicated by symptomatic hypotension was similar in the 2 groups. HFR-iso improved BP control without increasing dialysis hypotension episodes. Short Summary: In this multicenter, open-label, controlled, randomized study, we evaluated the impact of dialysis biofeedback in HFR on arterial hypertension compared to conventional HFR. We observed that HFR-iso improved arterial BP control without increasing dialysis hypotension episodes.
Serological responses to rotavirus NSP2 following administration of RV3-BB human neonatal rotavirus vaccine

ABSTRACT Serum rotavirus IgA responses are an imperfect non-mechanistic correlate of protection, and the lack of an accurate serological marker is a challenge to the development of new rotavirus vaccines. Serological responses to rotavirus NSP2 occur following wild-type infection; however, it is unknown if serological responses to NSP2 occur following administration of rotavirus vaccines. The phase IIa immunogenicity trial of RV3-BB provided an opportunity to investigate the serological responses to NSP2 following vaccination. Healthy, full-term babies (n = 96) were previously recruited as part of a phase IIa safety and immunogenicity trial in Dunedin, New Zealand between January 2012 and April 2014. Participants received three doses of oral RV3-BB vaccine with the first dose given at 0–5 days after birth (neonatal schedule), or the first dose given at about 8 weeks after birth (infant schedule), or to receive placebo (placebo schedule). Serum IgA and IgG antibody responses to total RV3-BB and NSP2 protein (RV3-BB) were assessed using ELISA. Despite significant serum IgA response against total RV3-BB, we were unable to demonstrate a significant serological response to NSP2 in participants receiving RV3-BB when compared to placebo. Heterotypic antibodies against multiple NSP2 genotypes were detected following RV3-BB vaccination. Our data demonstrates that while serological responses to NSP2 were detectable in a subset of participants, it is a less useful marker when compared to total rotavirus serum IgA response.
Percutaneous patent foramen ovale closure using Helex and Amplatzer devices without intraprocedural echocardiographic guidance.

OBJECTIVES We compared procedural outcomes of patients undergoing patent foramen ovale (PFO) closure using Helex (W.L. Gore & Assoc., Flagstaff, AZ, USA) and Amplatzer (AGA Medical Corp., Plymouth, MN, USA) devices using intracardiac echocardiographic (ICE) versus fluoroscopic-only guidance. BACKGROUND Use of transesophageal or ICE to guide PFO closure is associated with patient discomfort and cost. While fluoroscopic guidance of septal closure using Amplatzer is well established, there is no published experience for Helex. METHODS We performed a single-center, single-operator analysis of patients undergoing PFO closure using Helex or Amplatzer occluders. Device and guidance strategy was selected by the operator. RESULTS Of the 132 PFO patients, 23 were closed with Helex, and 109 were closed with Amplatzer (103 Cribriforms, 4 PFO occluders, and 2 atrial septal occluders). Fluoroscopic guidance was used for 15 (65%) Helex and 102 (94%) Amplatzer cases. Successful device placement was achieved in all patients with a 1.5% complication rate (1 arrhythmia and 1 device embolization). Procedure time was shorter for fluoroscopic guidance of Amplatzer cases compared to ICE guidance (P = 0.023), and for Amplatzer versus Helex cases (P = 0.0004). Among the Helex cases, there were no differences in procedure or fluoroscopy time comparing ICE to fluoroscopic guidance. There was no residual shunting by transthoracic echocardiographic bubble study in 93% of Helex and 95% of Amplatzer cases at 6 months. CONCLUSIONS Use of right atrial angiography and fluoroscopic-only guidance for PFO closure using Helex and Amplatzer devices provides an efficient alternative to ICE guidance. While procedure and fluoroscopy times were significantly shorter for Amplatzer versus Helex cases, these times were similar for Helex comparing fluoroscopy versus ICE guidance.
Evaluation of the cultivation conditions of marine microalgae Chlorella sp. to be used as feedstock in ultrasound-assisted ethanolysis

A total of 8 assays was conducted to study the influence of different variables namely, light intensity, CO2 level, NaNO3 concentration and aeration rate, on the cultivation of the marine microalgae Chlorella sp. to enhance the biomass feedstock availability for biodiesel. The experiments were designed using a Taguchi L8 experimental array set at two levels of operation, having light intensity (0.85 and 14.5 klux), CO2 (5 and 10%), NaNO3 (0.025 and 0.075 g L-1) and aeration rate (3:33 and 1.67 vvm) as independent variables and considering biomass productivity and lipid content as response variables. All the experiments were performed in six photobioreactor vessels connected in series with a total volume of 8.4 L and working volumes of 2 L and 4 L, depending on the conditions assessed. The highest biomass productivity was 210.9 mg L-1day-1, corresponding to a lipid content of 8.2%. Such results were attained when the culture conditions were set at 0.85 klux light intensity, 5% CO2 and 0.075 g L-1 NaNO3. The aeration rate showed no significant influence on the biomass productivity. On the other hand, the highest lipid content was achieved when the cultures were grown using the lowest concentration of NaNO3 (0.025 g L-1) and an aeration rate of 1.67 vvm, while the other factors had no statistical significance. Under these conditions, the lipid content obtained was 19.8%, at the expense of reducing the biomass productivity to 85.9 mg L-1day-1.The fatty acid profile of the lipid material characterized by gas chromatography identified fourteen fatty acids with carbon chain ranging from C8 to C20 in which most of the fatty acids present were saturated (58.7 %) and monounsaturated (36.1%) fatty acids. Those obtained at higher proportions were the oleic (22.8%), palmitic (20.7%) and lauric (17.7 %) acids, indicating a suitable composition for fatty acid ethyl esters (FAEE) synthesis. This was confirmed by acid catalysis performed under ultrasound irradiations reaching a conversion rate of 78.4% within only 4 h.
Simulation for High Frequency (HF) range of helical antenna

This paper is discusses on the designing of helical antenna in the High Frequency (HF) range from 3 MHz to 30 MHz. The software used for the simulation is the CST Microwave Studio (CST MWS) which is an analytical tool that provides an accurate 3D EM simulation results for high frequency design. Several parameters dimension and helical's profiles were studied in order to obtained a suitable resonance frequency. Among the helical antenna parameters that being studied were on its different forms that link to multiple diameters, radius ratio, and number of turns. In this paper, attention is focused on helical antennas which find important applications in communication systems, primarily because of their circular polarization and wide bandwidth. The helical antenna, first introduced by Kraus in 1946 [4], has been subject of extensive investigations during the past five decades. Many modifications to the basic helix geometry have been proposed with the aim of improving the gain, bandwidth, axial ratio, and VSWR. More recently, the possibility of size reduction, while maintaining the radiation characteristics, has been explored. Both the theoretical and simulation results will be presented.
Shedding & shaving: Disclosure of proteomic expressions on a bacterial face

Bacterial surface proteins are critically important in determining the success of bacterial strains in their competition for survival, which makes comprehensive knowledge of the microbial ‘face’ of high relevance to understand bacterial physiology. Therefore, the aim of this study was to inventorize the proteomic composition of a bacterial surface by gel‐free approaches using Bacillus subtilis as a model organism. To this purpose, intact bacteria were incubated with trypsin to cleave surface proteins, after which these ‘shaved’ proteins were identified by LC‐MS/MS analysis of tryptic peptides that were released into the incubation buffer. In parallel, proteins that were released from bacterial cells without trypsin treatment were inventorized and defined as ‘shed’ proteins. The results showed that peptides of 41 proteins, comprising transmembrane proteins, secretory and lipoproteins, known ‘anchorless’ surface proteins and ribosomal proteins, were specifically released by shaving. Some of these proteins were also cleaved by trypsin‐beads, which are unable to penetrate the bacterial cell wall, indicating a genuine surface‐exposed localization. Together this shows that these combined gel‐free approaches can reveal novel proteomic decorations on a bacterial face and may provide leads for future studies on protein sorting in B. subtilis and other Gram‐positive bacteria.
Phytosomal curcumin inhibits tumor growth in colitis‐associated colorectal cancer

Colorectal‐cancer (CRC) is the third leading cause of death due to cancer, supporting the need for identification of novel anticancer drug to improve the efficacy of current‐therapy. There is growing bodies of data showing the antitumor‐activity of curcumin, although it is associated with low absorption. The aim of current study was explored the therapeutic‐potential of novel phytosomal curcumin as well as its application in combination with 5‐Flurouracil (5‐FU) in a mouse‐model of colitis‐associated colon‐cancer. The anti‐proliferative‐activity of phytosomal curcumin was assessed in 2‐ and 3‐dimensional cell‐culture‐models as well as in a mouse‐model of colitis‐associated colon‐cancer. The expression‐levels of CyclinD1, beclin, E‐cadherin, and p‐GSK3a/b were investigated by qRT‐PCR and/or Western‐blotting. We evaluated the anti‐inflammatory of this agent by pathological‐evaluation and disease‐activity‐index (DAI). Moreover, oxidant/antioxidant activity was examined by malondialdehyde (MDA), total‐thiols (T‐SH), superoxide‐dismutase (SOD), and catalase (CAT) activity parameters. Our data showed that phytosomal curcumin and its combination with 5‐FU inhibited cell growth and invasive behavior of CRC cells through modulation of Wnt‐pathway and E‐cadherin. Combination of curcumin with 5‐FU dramatically reduced the tumor‐number and tumor‐size in both distal and middle parts of colon in colitis‐associated colon cancer followed by reduction in DAI. Also, curcumin suppressed the colonic inflammation and notably recovered the increased levels of MDA, decreased thiol level and reduced activity of CAT. We demonstrated the antitumor‐activity of novel form of curcumin in CRC, supporting further investigations on the therapeutic‐potential of this approach in colorectal‐cancer.
UiO-UvA at SemEval-2020 Task 1: Contextualised Embeddings for Lexical Semantic Change Detection

We apply contextualised word embeddings to lexical semantic change detection in the SemEval-2020 Shared Task 1. This paper focuses on Subtask 2, ranking words by the degree of their semantic drift over time. We analyse the performance of two contextualising architectures (BERT and ELMo) and three change detection algorithms. We find that the most effective algorithms rely on the cosine similarity between averaged token embeddings and the pairwise distances between token embeddings. They outperform strong baselines by a large margin (in the post-evaluation phase, we have the best Subtask 2 submission for SemEval-2020 Task 1), but interestingly, the choice of a particular algorithm depends on the distribution of gold scores in the test set.
[Diabetic neuropathy--a clinical review].

BACKGROUND Diabetic neuropathy is an often overlooked common complication of diabetes. METHODS This review is based on papers identified on Medline and our own clinical experience. RESULTS AND INTERPRETATION Diabetic neuropathies can be classified as reversible or chronic. The most common type is the chronic, progressive distal symmetric polyneuropathy where sensory symptoms in the lower limbs dominate. These patients often lose their protective sensibility and are at risk of foot ulcers and amputations; distressing pain is another symptom of this disorder. Autonomic neuropathy is often a feature of progressive polyneuropathy, but is rarely symptomatic. Mononeuropathies affecting femoral, truncal or ocular nerves are among the reversible neuropathies of diabetes. Distal symmetric diabetic neuropathy is very common; overall prevalence among diabetic patients is around 20-30%. The prevalence increases with the duration of the disease as well as with poor glycaemic control, height and age. The pathogenesis of diabetic neuropathies is not clear, but possibly involves a complex of metabolic factors inducing nerve ischaemia. We recommend yearly screening for most diabetic patients with a simple screening instrument consisting of four questions and four simple tests. Tight glycaemic control has been shown to protect against or delay the occurrence of neuropathy. Tricyclic antidepressants are still the drugs of choice against painful diabetic neuropathy, but gabapentin and tramadol are new alternatives.
Rätten till ersättning för gränsöverskridande vård och kravet på vetenskap och beprövad erfarenhet

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Role Burdens: The Impact of Employment and Family Responsibilities on the Health Status of Latino Women

This paper explores the relative effects of employment and family responsibility on the perceived health status of Latino women. The data source analyzed for this study was the 1990 Panel Study of Income Dynamics/Latino National Political Survey (PSID/LNPS) Early-Release File (n = 1,502). Regression analyses were used to investigate the contributions of variables associated with perceived health status, including sociodemographics, Latino ethnicity, language, employment, and family responsibility. The results suggest that annual employment hours, occupation, and family responsibilities, such as child care and weekly housework, significantly affect self-reported health status of employed Latinas. Both social causation and social selection may be underlying the associations found. The results suggest that there is need for the development of public policies that seek to increase Latinas' labor force participation rate since any expansion has the potential to have a positive impact on their health status.
Bone density at multiple skeletal sites in amenorrheic athletes.

OBJECTIVE To determine if there is a generalized loss of bone mass at multiple skeletal sites in amenorrheic athletes compared with a group of eumenorrheic athletes. DESIGN A case-control study examining the differences in bone mineral density (BMD) between amenorrheic and eumenorrheic athletes. SETTING Seattle, Wash, and surrounding communities. PARTICIPANTS Forty-nine athletes, aged 17 to 39 years, were selected from those responding to advertisements in local sporting-goods stores and a track-and-field newsletter. Athletes were defined as amenorrheic if they had had fewer than 2 menstrual cycles in the last 12 months or none in the past 6 months, or eumenorrheic if they had had 10 to 13 cycles in the previous year. Only women who met these criteria, confirmed by tests for estradiol and progesterone levels, were enrolled in the study. MAIN OUTCOME MEASURES Bone mineral density measured by dual-energy x-ray absorptiometry. RESULTS Amenorrheic athletes had significantly lower BMD (P < .01) at the lumbar spine, femoral neck, trochanter, Ward triangle, intertrochanteric region, femoral shaft, and tibia. No difference was noted at the fibula. Body weight combined with months of amenorrhea and age of menarche predicted the BMD of the lumbar spine for amenorrheic athletes. Duration of amenorrhea and body weight of amenorrheic athletes predicted BMD at the femoral neck, trochanter, intertrochanteric region, and tibia. Weight alone predicted BMD at the femoral shaft and tibia. Age plus weight predicted lumbar BMD of eumenorrheic women. CONCLUSION Extended periods of amenorrhea may result in low bone density at multiple skeletal sites including those subjected to impact loading during exercise.
Identification of human cytochrome P450 isoforms involved in the 3-hydroxylation of quinine by human live microsomes and nine recombinant human cytochromes P450.

Studies using human liver microsomes and nine recombinant human cytochrome P450 (CYP) isoforms (CYP1A1, 1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1 and 3A4) were performed to identify the CYP isoform(s) involved in the major metabolic pathway (3-hydroxylation) of quinine in humans. Eadie-Hofstee plots for the formation of 3-hydroxyquinine exhibited apparently monophasic behavior for all of the 10 different microsomal samples studies. There was interindividual variability in the kinetic parameters, as follows: 1.8-, 3.2- and 3.5-fold for K(m) Vmax and Vmax/K(m), respectively. The mean +/- S.D. values for K(m), Vmax and Vmax/K(m) were 106.1 +/- 19.3 microM, 1.33 +/- 0.48 nmol/mg protein/min and 12.8 +/- 5.1 microliters/mg protein/min, respectively. With 10 different human liver microsomes, the relationships between the 3-hydroxylation of quinine and the metabolic activities for substrates of the respective CYP isoforms were evaluated. The 3-hydroxylation of quinine showed an excellent correlation (r = 0.986, P < .001) with 6 beta-hydroxylation of testosterone, a marker substrate for CYP3A4. A significant correlation (r = 0.768, P < .01) between the quinine 3-hydroxylase and S-mephenytoin 4'-hydroxylase activities was also observed. However, no significant correlation existed between the 3-hydroxylation of quinine and the oxidative activities for substrates for CYP1A2 (phenacetin), 2C9 (diclofenac), 2D6 (desipramine) and 2E1 (chlorzoxazone). Ketoconazole and troleandomycin (inhibitors of CYP3A4) inhibited the 3-hydroxylation of quinine by human liver microsomes with respective mean IC50 values of 0.026 microM and 28.9 microM. Anti-CYP3A antibodies strongly inhibited quinine 3-hydroxylation, whereas weak inhibition was observed in the presence of S-mephenytoin or anti-CYP2C antibodies. Among the nine recombinant human CYP isoforms, CYP3A4 exhibited the highest catalytic activity with respect to the 3-hydroxylation of quinine, compared with the minor activity of CYP2C19 and little discernible or no effect of other CYP isoforms. Collectively, these data suggest that the 3-hydroxylation of quinine is mediated mainly by CYP3A4 and to a minor extent by CYP2C19. Other CYP isoforms used herein appear to be of negligible importance in this major pathway of quinine in humans.
Modification of Nonstructural Protein 1 of Influenza A Virus by SUMO1

ABSTRACT Nonstructural protein 1 (NS1) is one of the major factors resulting in the efficient infection rate and high level of virulence of influenza A virus. Although consisting of only approximately 230 amino acids, NS1 has the ability to interfere with several systems of the host viral defense. In the present study, we demonstrate that NS1 of the highly pathogenic avian influenza A/Duck/Hubei/L-1/2004 (H5N1) virus interacts with human Ubc9, which is the E2 conjugating enzyme for sumoylation, and we show that SUMO1 is conjugated to H5N1 NS1 in both transfected and infected cells. Furthermore, two lysine residues in the C terminus of NS1 were identified as SUMO1 acceptor sites. When the SUMO1 acceptor sites were removed by mutation, NS1 underwent rapid degradation. Studies of different influenza A virus strains of human and avian origin showed that the majority of viruses possess an NS1 protein that is modified by SUMO1, except for the recently emerged swine-origin influenza A virus (S-OIV) (H1N1). Interestingly, growth of a sumoylation-deficient WSN virus mutant was retarded compared to that of wild-type virus. Together, these results indicate that sumoylation enhances NS1 stability and thus promotes rapid growth of influenza A virus.
Genome Analysis of Chlamydomonas reinhardtii Reveals The Existence of Multiple, Compartmentalized Iron–Sulfur Protein Assembly Machineries of Different Evolutionary Origins

The unicellular green alga Chlamydomonas reinhardtii is used extensively as a model to study eukaryotic photosynthesis, flagellar functions, and more recently the production of hydrogen as biofuel. Two of these processes, photosynthesis and hydrogen production, are highly dependent on iron–sulfur (Fe–S) enzymes. To understand how Fe–S proteins are assembled in Chlamydomonas, we have analyzed its recently sequenced genome for orthologs of genes involved in Fe–S cluster assembly. We found a total of 32 open reading frames, most single copies, that are thought to constitute a mitochondrial assembly pathway, mitochondrial export machinery, a cytosolic assembly pathway, and components for Fe–S cluster assembly in the chloroplast. The chloroplast proteins are also expected to play a role in the assembly of the H-cluster in [FeFe]-hydrogenases, together with the recently identified HydEF and HydG proteins. Comparison with the higher plant model Arabidopsis indicated a strong degree of conservation of Fe–S cofactor assembly pathways in the green lineage, the pathways being derived from different origins during the evolution of the photosynthetic eukaryote. As a haploid, unicellular organism with available forward and reverse genetic tools, Chlamydomonas provides an excellent model system to study Fe–S cluster assembly and its regulation in photosynthetic eukaryotes.