Miracle-dz/UniFAR-FTUs huggingface dataset:
Differential mutagen sensitivity of peripheral blood lymphocytes from smokers and nonsmokers: Effect of human cytomegalovirus infection

We used the mutagen sensitivity assay to test the hypothesis that human cytomegalovirus (HCMV) infection modifies the sensitivity of cells to genetic damage from genotoxic agents. Chromosome aberration (CA) frequency in peripheral blood lymphocytes (PBLs) from 20 smokers who were matched with 20 nonsmokers by age (± 5 years), sex, and ethnicity was evaluated following in vitro exposure to bleomycin and/or HCMV infection. Bleomycin induced significant (P < 0.05) concentration‐dependent increases in the frequency of aberrant cells, chromatid‐type damage (breaks), and chromosome‐type aberrations (deletions, rearrangements) in PBLs. The baseline (background) CA frequency was similar in both smokers and nonsmokers. Significantly higher frequencies of aberrant cells (P < 0.05) were observed in PBLs from smokers compared to nonsmokers at all bleomycin concentrations tested (10, 30 and 100 μg/ml). Infection of PBLs with HCMV induced a significant (P < 0.05) twofold increase in the frequency of CA (primarily chromatid breaks) in PBLs, regardless of the smoking status. PBLs from smokers and nonsmokers infected with HCMV prior to challenge with bleomycin demonstrated significant (P < 0.05) concentration‐dependent increases in the levels of aberrant cells, chromatid‐type damage (breaks), and chromosome‐type aberrations (deletions, rearrangements) compared to noninfected cells challenged with bleomycin. The frequency of induced CA was consistently higher for PBLs derived from smokers relative to nonsmokers (P = 0.06 and 0.002). These data indicate that, individually, both smoking and HCMV infection significantly enhance the sensitivity of PBLs to bleomycin‐induced genetic damage. More importantly, the data also suggest that smoking and HCMV infection interact synergistically to enhance the sensitivity of PBLs to such damage. Environ. Mol. Mutagen. 43:169–178, 2004. © 2004 Wiley‐Liss, Inc.
Characterization of vir-activated TnphoA gene fusions in Bordetella pertussis

The expression of many of the known virulence determinants of Bordetella pertussis is coordinately regulated by the vir regulatory locus and reduced in response to environmental signals called modulators. We have previously identified eight TnphoA gene fusions in B. pertussis in which the expression of alkaline phosphatase was maximal in the absence of the modulators nicotinic acid and MgSO4. We have termed the genes identified by these fusions vir-activated genes. Here we report the characterization of these TnphoA mutant strains. Four fusion strains were defective in known virulence determinants. For one of these, fusion strain SK39, Southern blot hybridization demonstrated that TnphoA was inserted in the S1 subunit gene of pertussis toxin. Hemagglutination assays, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblots identified three fusions strains, SK16, SK75, and SK91, that were defective in filamentous hemagglutinin. Whereas all three filamentous hemagglutinin-defective mutants showed either normal or enhanced colonization, the pertussis toxin-defective mutant showed a marked defect in pulmonary persistence. Of the four other fusion strains, two were deficient in outer membrane proteins. One of these, strain SK8, was defective in a major outer membrane protein of 95 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This strain colonized mouse lungs less well and did not induce lymphocytosis after aerosol challenge. The other strain, SK34, was defective in four outer membrane proteins, three of which were detectable only on a Western blot with polyclonal sera against B. pertussis. Two of our gene fusion strains did not show any defect in identifiable vir-regulated proteins.
Use of an Innovative Web-Based Laboratory Surveillance Platform to Analyze Mixed Infections Between Human Metapneumovirus (hMPV) and Other Respiratory Viruses Circulating in Alberta (AB), Canada (2009–2012)

We investigated the proportions of mono vs. mixed infections for human metapneumovirus (hMPV) as compared to adenovirus (ADV), four types of coronavirus (CRV), parainfluenza virus (PIV), RSV, and enterovirus/rhinovirus (ERV) in Alberta, Canada. Using the Data Integration for Alberta Laboratories (DIAL) platform, 26,226 respiratory specimens at ProvLab between 1 July 2009 and 30 June 2012 were selected and included in the study. Using the Respiratory Virus Panel these specimens tested positive for one or more respiratory virus and negative for influenza A and B. From our subset hMPV was the fourth most common virus (n=2,561) with 373 (15%) identified as mixed infection using DIAL. Mixed infection with hMPV was most commonly found in infants less than 6 months old and ERV was most commonly found in mixed infection with hMPV (230/373, 56%) across all age groups. The proportion of mixed-infection vs. mono-infection was highest for ADV (46%), followed by CRV 229E (32%), CRV HKU1 (31%), CRV NL63 (28%), CRV OC43 (23%), PIV (20%), RSV (17%), hMPV (15%) and ERV (13%). hMPV was significantly more likely to be identified in mono infection as compared with ADV, CRV, PIV, and RSV with the exception of ERV [p<0.05].
The diagnostic reliability of urinary cytology: A retrospective study

The aim of this study was twofold. The first aim was to estimate the diagnostic reliability of urinary cytology for detection and management of urothelial neoplasms by using a specific preserving fluid for sample collection, and the liquid‐based thin layer method for specimen preparation, the estimate was based on the correlation between the cytological findings of 10,000 non‐hospitalized patients, and their histological diagnoses. A second aim was to compare the reliability of two instruments for thin‐layer preparation, i.e., TP2000, TP3000, capable of processing the specimens at very different rates. The preservation of cell structure is ameliorated by the procedure of sample collection and treatment here described. This allows a more accurate reading of LBC slides as shown by: (a) the significant concordance between cytological and histological diagnosis (92%); (b) the significant number of low‐grade urothelial carcinomas (20.5%) revealed by urinary cytology and validated by histologic diagnosis; (c) the low rate (8%) of misjudgement of cytological diagnosis reached in this study. The quality of performances of the two instruments tested for thin‐layer preparation, i.e., TP2000 and TP3000, is statistically comparable. We recommend the procedure that makes use of preserving fluid for sample collection (cytolyt™) and treatment (preservcyt ™) as here described. We also recommend the use of thin‐layer method for specimen preparation since it allows a more uniform distribution of the cells on the support with reduction of overlapping phenomena. Finally, economic considerations suggest the preferential use of Thin Prep 3000. Diagn. Cytopathol. 2012. © 2011 Wiley Periodicals, Inc.
Management Expectations and Asymmetric Cost Behavior

The documentation of asymmetric cost behavior in response to changes in demand has attracted much scholarly attention over the past decade. Most studies suggest that this cost asymmetry is due to the influence of management expectations on their deliberate resource allocation decisions. Our study contributes to this research by providing direct empirical evidence in support of this explanation. Using the tone in the forward-looking statements (FLS) of a sample of 10-K reports as a measure of management expectations, we find a positive and significant relation between the favorableness of management FLS tone and the degree of cost stickiness. In addition, we examine the interaction between management expectations and the amount of slack resources available. When the amount of slack resources is high, we find that negative expectations result in anti-stickiness, whereas positive expectations result in sticky cost behavior. Accordingly, we find that managers’ expectation-driven decisions can reverse the previously documented anti-sticky cost behavior imposed by high slack resources. Finally, we find that the impact of management expectations on the degree of cost asymmetry is strongest when both the initial amount of slack resources and the magnitude of the adjustment costs are high. Conversely, when both the magnitude of the adjustment costs and the initial amount of slack resource are low, management expectations have no impact on the degree of cost asymmetry. Our combined evidence supports the explanation that management expectations influence their resource allocation decisions, and indicates that other economic determinants may need to be considered when assessing the impact of these decisions on a firm’s cost structure.
Vosaroxin in combination with decitabine in newly diagnosed older patients with acute myeloid leukemia or high-risk myelodysplastic syndrome

Vosaroxin is an anti-cancer quinolone-derived DNA topoisomerase II inhibitor. We investigated vosaroxin with decitabine in patients ≥60 years of age with newly diagnosed acute myeloid leukemia (n=58) or myelodysplastic syndrome (≥10% blasts) (n=7) in a phase II non-randomized trial. The initial 22 patients received vosaroxin 90 mg/m2 on days 1 and 4 with decitabine 20 mg/m2 on days 1–5 every 4–6 weeks for up to seven cycles. Due to a high incidence of mucositis the subsequent 43 patients were given vosaroxin 70 mg/m2 on days 1 and 4. These 65 patients, with a median age of 69 years (range, 60–78), some of whom with secondary leukemia (22%), adverse karyotype (35%), or TP53 mutation (20%), are evaluable. The overall response rate was 74% including complete remission in 31 (48%), complete remission with incomplete platelet recovery in 11 (17%), and complete remission with incomplete count recovery in six (9%). The median number of cycles to response was one (range, 1–4). Grade 3/4 mucositis was noted in 17% of all patients. The 70 mg/m2 induction dose of vosaroxin was associated with similar rates of overall response (74% versus 73%) and complete remission (51% versus 41%, P=0.44), reduced incidence of mucositis (30% versus 59%, P=0.02), reduced 8-week mortality (9% versus 23%; P=0.14), and improved median overall survival (14.6 months versus 5.5 months, P=0.007). Minimal residual disease-negative status by multiparametric flow-cytometry at response (± 3 months) was achieved in 21 of 39 (54%) evaluable responders and was associated with better median overall survival (34.0 months versus 8.3 months, P=0.023). In conclusion, the combination of vosaroxin with decitabine is effective and well tolerated at a dose of 70 mg/m2 and warrants randomized prospective evaluation. ClinicalTrials.gov: NCT01893320
Relationship Lending Through the Cycle: What Can We Learn from Three Decades of Research?

This survey on Relationship Lending (RL) reviews thirty years of literature in a new analysis framework that considers the alleged benefits of RL in all possible scenarios under which banks and firms could operate from a good time scenario to a scenario of generalized crisis affecting both firms and banks. The number of studies currently conducted on RL in a scenario of prolonged crisis is growing rapidly, confirming that RL remains a viable solution and that it helps both banks and their customers to weather the storm. We also add suggestions on additional dimensions of RL that could be investigated in empirical studies to capture the presence of RL in the credit policy of each bank more effectively and thus better measure its effects on both sides – firm and bank – of the relationship.
Revisiting Trade Unions’ Response to New Public Management: A Case From Zambia

This article discusses the strategic responses by public-sector unions to new public management (NPM) reforms in Zambia. The article is based on a qualitative research methodology focusing on the Civil Servants and Allied Workers Union of Zambia. The study shows that public-sector workers in developing countries are more vulnerable to the effects of externally imposed NPM reforms, which include job cuts. However, the implementation of these reforms faces opposition especially from trade unions. In line with the assumptions of strategic choice theory, union responses to NPM reforms are strategic. Despite the high vulnerability of public workers in developing countries, their unions use strategies that can also be observed in developed countries to mitigate the negative consequences of NPM reforms on the public sector. These strategies follow a three-stage process, namely, opposing the reforms, negotiating for favorable reform measures, and shifting from centralized structures to networks.
Non-pharmacological conservative therapy for phantom limb pain: A systematic review of randomized controlled trials

ABSTRACT The aim of this manuscript was to investigate the effectiveness of conservative therapy for phantom limb pain (PLP). In this systematic review, CINAHL, AMED, the Cochrane database of systematic reviews, PEDro, psychology and behavioral sciences collection, and MEDLINE were systematically searched for appropriate randomized controlled trials (RCTs). Selected papers were assessed for risk of bias, and evidence was graded using the GRADE approach. Twelve RCTs met initial inclusion/exclusion criteria, of which five were of sufficient quality for final inclusion. There is conflicting evidence from two RCTs for the effectiveness of electromagnetic shielding limb liners on pain in the short term. There is limited evidence supporting the effectiveness of both hypnosis in the short term and graded motor imagery (GMI) in the short-to-medium term. Additionally, there is limited evidence that a single session of mirror therapy has no immediate effect on PLP. Limb liner discomfort was the only adverse effect identified. This review identifies a range of conservative therapies, many of which demonstrate preliminary evidence of potential with respect to clinically worthwhile effects above control interventions and few, if any, adverse effects. However, there is a paucity of high-quality evidence upon which to make any firm clinical conclusions.
Association between restless leg syndrom and slow coronary flow.

OBJECTIVE Restless legs syndrome (RLS) is a common sleep disorder in which patients feel unpleasent leg sensations and urge to move the legs during rest, especially at night, and symptoms are improved by leg movement. Prior studies analyzing the associations between cardiovascular disease and restless legs syndrome has shown controversial results. The goal of the study was to estimate the relationship between restless legs syndrome and slow coronary flow (SCF). METHODS The present study was cross-sectional and observational and consists of 176 individuals who underwent coronary angiography and had angiographically normal coronary arteries of varying coronary flow rates. The study included 86 patients with isolated SCF and 90 control participants with normal coronary flow (NCF). RLS was assessed the day after the coronry flow was evaluated, using a self-administered questionnaire based on the International Restless Legs Study Group criteria. The following question was asked: "Do you have unpleasant leg sensations (like crawling, paraesthesia, or pain) combined with motor restlessness and an urge to move? " The possible responses were as follows: no, less than once/month, 2-4 times/month, 5-14 times/month, and 15 or more times per month. Those who answered that they had these feelings were asked the following two more questions: 1) "Do these symptoms occur only at rest and does moving improve them? " and 2) "Are these symptoms worsen in the evening/at night compared with the morning? " RLS is considered to be probable if the participant has answered "yes" for all three of the above questions, and has a frequency of ≥5 times/month. Student's t-test, Mann-Whitney U test, multiple logistic regression analysis were used for statistical analysis. RESULTS The prevalence of restless legs syndrome was 48 (27%) and increased significantly with age. Patients with SCF have more likely had RLS than the control group (p<0.001). The age-adjusted prevalence odds of SCF were 3.11 times higher (95% CI: 1.54-6.29; P<0.001) among patients with RLS symptoms. Significant associations between RLS and SCF did not materially change after further adjustment for other potential covariates, such as sex, BMI, BMI squared, smoking. CONCLUSION Our study concluded that there is a strong link between SCF and RLS.
Bisphosphonate therapy in the oncology setting

Patients with metastatic cancer and bone involvement are at chronic risk of skeletal complications, including bone pain, fractures, spinal cord compression and hypercalcaemia of malignancy. Therapies targeting the primary malignancy are often unable to prevent skeletal complications, which often require orthopaedic surgery, radiation therapy and analgesics. Intravenous bisphosphonates can reduce the risk of skeletal complications and the requirement for palliative radiation therapy. Since its broad regulatory approval, zoledronic acid (ZOMETA®, Novartis Pharma AG/Novartis Pharmaceuticals Corporation) 4 mg by 15-minute intravenous infusion has become widely used to treat bone metastases from all solid tumours and is becoming the standard of care for advanced breast cancer and multiple myeloma. Additionally, cancer treatment-induced bone loss is an emerging problem in clinical oncology, and bisphosphonates – particularly intravenous bisphosphonates – may provide benefits even before bone lesions develop. Further investigations of bisphosphonates in these and other indications are ongoing.
Psycho‐Social Factors In Pelvic Pain: A Controlled Study Of Women Living In Physically Abusive Relationships

The study was designed to reveal possible associations between living in an abusive relationship and gynecological disturbances, including pelvic pain. 66 women living in or having lived in such a relationship the previous year were compared with 114 women, in a random sample of women from the same area not exposed to life in an abusive relationship. Data from both groups were obtained through personal interview. 32 women (48%) in the study group and 24 (21%) among controls had a history of pelvic pain (p<0.001). Eight (12%) women in the study‐group versus 2 (2%) of the controls had contacted a physician and reported a history of pelvic pain during the year prior to the interview (p < 0.05). The difference in the history of pelvic pain could not be explained by differences in psychosocial factors between the groups.
Assessing Impact of Quota Elimination on Factor Productivity Growth of Textile Sector of Pakistan

Export of textile sector of Pakistan is also dependent on the trade policy of importing countries, particularly European Union and USA, as these countries have been major importer of textile goods of Pakistan. This study is unique in the way that it contributes in existing literature by estimating factor productivity growth of textile sector in the perspective of pre quota and post quota elimination situation, as this has been ignored in past. We explored that textile sector of Pakistan did not get benefit of quota elimination and productivity remained almost stagnant over the period. We suggest penetrating textile export in unrestricted markets apart from European Union and USA and closure of sick units and outdated technology.
Development of competitive ELISAs for 17β-estradiol and 17β-estradiol +estrone+estriol using rabbit polyclonal antibodies

Estrogens are a family of feminizing hormones that are excreted by vertebrates. It has been documented that their presence in surface waters, even in the ng/L range, can have detrimental impacts on fish reproduction. Two competitive enzyme-linked immunosorbent assays using rabbit polyclonal antibodies were developed: one for 17β-estradiol and a second one for 17β-estradiol (E2)+estrone (E1)+estriol (E3). Two different conjugates were synthesized using the Mixed-anhydride (for the 17β-estradiol ELISA) and the Mannich (for the E1 + E2 + E3 ELISA) reactions. The 17β-estradiol ELISA was highly specific with an IC50 of 243 ng/mL for 17β-estradiol. The E1 + E2 + E3 ELISA exhibited cross-reactivity with estrone (85%) and estriol (62%) with an IC50 of 18 ng/mL for 17β-estradiol. Cross-reactivity was tested against 13 chemically related compounds and both immunoassays showed significant cross-reactivity with two estradiol conjugates: β estradiol-17-valerate and β estradiol-3-benzoate (from 57 to 84 %) for which, to our knowledge, there are currently no commercially available ELISA. Characteristics (sensitivity, inter and intra assay variation, and cross-reactivity) of the E1 + E2 + E3 ELISA were further compared to those from a commercial Estriol ELISA. The commercial ELISA was more specific, sensitive and its inter-assay variation was less (9.5% compared to 10% for the E1 + E2 + E3 ELISA) but the E1 + E2 + E3 ELISA had less intra-assay variation (4% compared to 5% for the commercial ELISA). Finally, a solid-phase extraction method compatible with the E1 + E2 + E3 immunoassay demonstrated that this combined approach of extraction and immunoassay had good potential for determining estrogen concentrations in environmental samples such as surface water in urban and agricultural ecosystems.
The Role of Power and Incentives in Inducing Fake Reviews in the Tourism Industry

As online reviews have become increasingly prevalent in recent years and their influence on consumers’ purchasing decisions has grown exponentially, some companies have begun to ask people to write fake reviews about their businesses or their competitors while offering compensation in return. This process has drawn the attention of regulators because it knowingly misleads consumers. This article reports on two studies that looked at the effect of two types of incentives (self-benefiting or charitable) on individuals’ intentions to write fake reviews and examined the moderating role of a person’s sense of power on his or her propensity to post a fake review. The study findings indicate that powerless individuals are more likely to post a fake review when presented with a monetary incentive rather than a charity incentive, while powerful individuals are not impacted by incentive type. Moreover, when asked to post negative fake reviews about competitors, such effects are mitigated.
Design and control of an exoskeleton for the elderly and patients

Recently, the exoskeletal power assistive equipment, which is a kind of wearable robot, has been widely developed to help human body motion. The exoskeleton for elderly people and patients, however, has some limitations due to the weight and volume of the equipment. In this paper, a tendon-driven exoskeletal power assistive device, exoskeleton for patients and the old by the Sogang University (EXPOS), is proposed as a feasible solution. In case of EXPOS, the caster walker carries heavy items such as motors, drivers, controllers, and batteries so that the weight and volume of the wearable exoskeleton are minimized. The tendon-connecting motors and pulleys of hip and knee generate the assistive power according to the requirement of the users. In this paper, the design concepts of the EXPOS, sensing techniques, and control methods are discussed