Miracle-dz/UniFAR-FTUs huggingface dataset:
The p97 antigen is mapped to the q24-qter region of chromosome 3; the same region as the transferrin receptor.

Since the p97 antigen, a membrane-associated iron-binding protein, has extensive amino acid sequence with homology with transferrin, is functionally related to the transferrin receptor, and has been previously mapped to chromosome 3, we have performed additional studies for regional mapping of the gene expressing p97 antigen. In these experiments, Chinese hamster-human cell lines were chosen that contained a large spectrum of autosomal human chromosomes, but mainly consisted of clones expressing all or a part of chromosome 3. These cell lines included a clone that previously allowed for mapping of human transferrin receptor to q22-qter region. Human p97 expression was assessed by specific binding of [125I]monoclonal antibody 96.5, and human transferrin receptor expression was tested by specific [125I]human transferrin binding and [125I]monoclonal antibody OKT-9 specific for human transferrin receptor. Based on these analyses, both human p97 antigenic expression and human transferrin receptor are mapped concordantly to the q24-qter region. These data and previous reports, therefore, suggest that the related iron-transport proteins are closely linked and may be under coordinate regulation. However, studies of several cell lines that exhibit up-regulation of human transferrin receptor expression with cellular proliferation, and down-regulation of receptor with increased transferrin-iron in the media, showed no change in expression of p97 antigen. p97 antigenic expression increased when melanocyte-stimulating hormone was added to a human melanoma cell line in tissue culture. These latter studies suggest that in mammalian cells the two proteins do not show coordinate regulation. (ABSTRACT TRUNCATED AT 250 WORDS)
Hypokalemic respiratory arrest in diabetic ketoacidosis.

THE OCCURRENCE of life-threatening hypokalemic hypoventilatory respiratory failure requiring intubation and respiratory support in diabetic ketoacidosis (DKA) is exceedingly rare. In none of the reported cases have serum phosphate levels been assessed within 12 hours of respiratory failure and in only one case have serial arterial blood gas measurements been performed to document hypoventilation. 1 The recent documentation of severe hypophosphatemia as a cause of hypoventilation and the fact that decrements in serum phosphate and serum potassium levels frequently parallel one another in DKA call into question the importance of hypokalemia to the respiratory response in DKA. We report a case of DKA in a young, otherwise healthy man whose treatment was complicated by severe hypokalemia and a hypoventilatory respiratory arrest without severe hypophosphatemia. We further discuss issues relating to the assessment and treatment of the hypokalemic patient with DKA at risk for ventilatory failure. Report of a Case
Estimation of carrier frequency offsets for uplink OFDMA system using a hybrid Taguchi-mutated-particle swarm optimization approach

In orthogonal frequency division multiple access (OFDMA) uplink systems, carrier frequency offsets (CFOs) caused by multiple users may significantly degrade system performance. Therefore, many approaches have recently been proposed to estimate the CFOs, such as Simplified Maximum Likelihood (SML) Scheme and Fast Algorithm. While those algorithms reveal acceptable performance, their computational burdens are still very high. For reducing the computational burden, a new particle swarm optimization (PSO)-based scheme is proposed in this study by introducing the mutation operation and Taguchi method. Experimental results indicate that the proposed approach can achieve performance close to SML and Fast Algorithm with much less computational burden.
Investigation of time-dependent forces on a nano-Newton-second impulse balance

A torsional impulse balance has been developed as a new diagnostic tool to study fundamental physical processes in micropropulsion systems and laser–surface interactions. The impulse balance has been designed and tested with a robust calibration system to measure impulsive forces with resolution as low as several nano-Newton-seconds. The behavior of the impulse balance was thoroughly studied and characterized. A simple analytical model of the balance’s motion was developed from the general equation of motion of an underdamped, harmonically oscillating system. Also, two distinct methods of analyzing the experimental data from the nano-impulse balance have been investigated. The first method resolves the total impulse as a function of the balance’s maximum deflection. The second method enables the determination of the impulse and/or force applied as a function of time from the balance’s time-resolved motion. A calibration scheme employing electrostatic actuation techniques is used to experimentally validate.
The role of pyridoxal 5'-phosphate in plant phosphorylase.

The removal of pyridoxal 5'-phosphate from potato phosphorylase [EC 2.4. 1.1] was achieved by incubation in an acidic ammonium sulfate solution containing hydroxylamine. Potato apophosphorylase is catalytically inactive, but reactivated by incubation with pyridoxal phosphate. Upon titration, the degree of recovery of activity agreed well with the degree of incorporation of pyridoxal phosphate. Therefore, it can be concluded that pyridoxal phosphate in the plant enzyme is the cofactor required for the enzyme activity, as it has been shown to be in the animal enzyme. The reconstitution of the apoenzyme using six pyridoxal phosphate analogues modified at the 5' position indicates that the structural properties of the cofactor binding site is similar in potato and rabbit muscle phosphorylases. This suggests that the plant enzyme has also a substrate binding site neighboring with the 5'-phosphate moiety of the bound cofactor similar to that shown to exist in the animal enzyme. On the other hand, the analysis of the far-ultraviolet circular dichroism spectra of these two enzymes shows that the secondary structures are rather different from each other; the potato enzyme is estimated to have 16% alpha-helix and 37% beta-structure, and the muscle enzyme b 41% alpha-helix and 21% beta-structure. This indicates that the cofactor binding locus must have been more strictly conserved than other regions.
Validity of clinic blood pressure compared to ambulatory monitoring in hypertensive patients in a primary care setting

Abstract Aims. To determine the validity of the blood pressure (BP) measurement in a primary care setting in comparison with the measurement obtained from 24-h ambulatory blood pressure monitoring (ABPM). Methods. Patients with hypertension (n = 137) were studied in primary care. Immediately after a nurse took two measurements of each patient's BP, the ABPM device was attached. Agreement was determined using the Bland–Altman method and the Kappa index. The sensitivity, specificity, predictive values and likelihood ratios were calculated for the manual BP measurement in comparison with ABPM for the purpose of diagnosing uncontrolled BP. Results. The BP values from ABPM were lower than those obtained in the examination room. The difference between the clinic measurement and the ABPM was 18.07 ± 14.6 mmHg (systolic blood pressure, SBP) and 6.3 ± 9.2 mmHg (diastolic blood pressure, DBP). The agreement between the clinic measurement and 24h-ABPM for the purpose of classifying control or uncontrolled BP was 64.2% (Kappa = 0.311). In the case of ABPM, the sensitivity of the measurement in the examination room for diagnosing uncontrolled BP was 80.36%, specificity was 53.09%, positive predictive value 54.22% and negative predictive value 70.63%. Conclusions. Clinic measurements do not have sufficient sensitivity/specificity to be recommended as a single method of BP control in primary care.
Glomerular crescents are responsible for chronic graft dysfunction in post‐transplant IgA nephropathy

Information is limited regarding the histological features related to chronic graft dysfunction and failure in patients with IgA nephropathy developing after renal transplantation. The prevalence and significance of glomerular crescents in post‐transplant IgAN including recurrent, de novo and transmitted forms (TxIgAN), were studied. Renal morphology was evaluated in 71 patients of TxIgAN, obtained at more than 6 months post‐transplant, and compared with regard to the presence (C‐TxIgAN) or absence (N‐TxIgAN) of glomerular crescents. Crescents were demonstrated in 12 samples of 10 patients (14.1%). The percentages of crescents were from 4.8% to 83.3% (median, 28.6%) in each sample. Ten samples of C‐TxIgAN had cellular to fibrocellular crescents, and four of these were associated with diffuse mesangial proliferation. Serum creatinine levels and the frequency of nephrotic range proteinuria at the time of biopsy and the degree of interstitial inflammation were significantly different in the two groups. Graft survival after allograft biopsies was significantly lower in C‐TxIgAN (P = 0.0017). Chronic rejection was a major cause of graft loss in N‐TxIgAN (31.8%), whereas TxIgAN was the major cause in C‐TxIgAN (66.7%). In conclusion, the current study suggests that glomerular crescents are not rare and that they are responsible for chronic graft dysfunction in TxIgAN patients.
Diagnosis of Progressive Multifocal Leukoencephalopathy by Brain Biopsy with Biotin Labeled DNA: DNA In situ Hybridization

DNA:DNA in situ hybridization using a cloned JC virus (JCV) DNA probe labeled with biotin confirmed the presence of JCV DNA in formalin-fixed, paraffin-embedded brain biopsies from four cases of progressive multifocal leukoencephalopathy (PML). Only small pieces of tissue were available in each case. Detection of the JC DNA:DNA hybrids was carried out by affinity cytochemistry. JCV DNA was identified predominantly in the nuclei of interfascicular oligodendrocytes in demyelinated areas of the biopsies. JC virus was isolated from one case, and the diagnosis of PML was substantiated in all cases by electron microscopic identification or immunocytochemical labeling of JC viral antigen. In situ hybridization using a biotin labeled JCV DNA probe is a specific, sensitive and convenient method for confirming the diagnosis of PML in suspected cases evaluated by brain biopsy.
Neoadjuvant Therapy of Esophageal Squamous Cell Carcinoma: Response Evaluation by Positron Emission Tomography

ObjectiveTo evaluate the use of positron emission tomography using [18F]-fluorodeoxyglucose (FDG-PET) to assess the response to neoadjuvant radiotherapy and chemotherapy in patients with locally advanced esophageal cancer. Summary Background DataImaging modalities, including endoscopy, endoscopic ultrasound, computed tomography, and magnetic resonance imaging, currently used to evaluate response to neoadjuvant treatment in esophageal cancer do not reliably differentiate between responders and nonresponders. MethodsTwenty-seven patients with histopathologically proven squamous cell carcinoma of the esophagus, located at or above the tracheal bifurcation, underwent neoadjuvant therapy consisting of external-beam radiotherapy and 5-fluorouracil as a continuous infusion. FDG-PET was performed before and 3 weeks after the end of radiotherapy and chemotherapy (before surgery). Quantitative measurements of tumor FDG uptake were correlated with histopathologic response and patient survival. ResultsAfter neoadjuvant therapy, 24 patients underwent surgery. Histopathologic evaluation revealed less than 10% viable tumor cells in 13 patients (responders) and more than 10% viable tumor cells in 11 patients (nonresponders). In responders, FDG uptake decreased by 72% ± 11%; in nonresponders, it decreased by only 42% ± 22%. At a threshold of 52% decrease of FDG uptake compared with baseline, sensitivity to detect response was 100%, with a corresponding specificity of 55%. The positive and negative predictive values were 72% and 100%. Nonresponders to PET scanning had a significantly worse survival after resection than responders. ConclusionFDG-PET is a valuable tool for the noninvasive assessment of histopathologic tumor response after neoadjuvant radiotherapy and chemotherapy.
DNA typing suggests pigeon droppings as a source of pathogenic Cryptococcus neoformans serotype D

The unusual proportion of serotype D strains of Cryptococcus neoformans infecting patients diagnosed with cryptococcosis in some parts of France prompted the analysis of DNA fingerprints obtained with 26 clinical and 29 environmental isolates from the same area. Our results suggest that pigeon droppings are a potential source of pathogenic strains of C. neoformans serotype D, as previously demonstrated for serotype A.
COMPORTAMENTO INGESTIVO DE OVINOS EM PASTAGENS: uma revisão

A producao de ovinos no Brasil ocorre em sua maioria a pasto e varios fatores podem influenciar o comportamento ingestivo destes animais, afetando direta e indiretamente a produtividade. A ma utilizacao das pastagens e falhas no manejo nutricional do rebanho sao exemplos de situacoes que determinam prejuizos economicos a ovinocultura. Os ovinos apresentam particularidades no comportamento ingestivo e estudos sobre os componentes deste, tais como periodo e duracao de pastejo, preferencia alimentar, taxa, peso e volume de bocado, e a aplicabilidade de seus efeitos nos sistemas de producao podem auxiliar a evitar tais prejuizos. Desta forma, o objetivo desta revisao foi discutir os principais itens que interferem no comportamento ingestivo de ovinos em pastagens. O conhecimento desses fatores e de grande importância para que se realize manejo mais apropriado na producao animal, possibilitando a expressao das caracteristicas comportamentais dos animais de modo que promova maiores ganhos em producao. Palavras-chave: Bocado; Ovinocultura; Pastejo; Seletividade.
Hippocampal Volume Measurements Using Magnetic Resonance Imaging in Normal Young Adults

Volumetric analysis of brain magnetic resonance images (MRIs) measures structural changes associated with neurological and neuropsychiatnc disorders. Several studies investigated the hippocampus specifically, report1ng degrees of atrophy in such disorders. However, the range of normal hippocampal volumes must be known to assess atrophy. In tracings of T1 oblique slice and three‐dimensional MRis 1n 24 normal subjects reported here, the average volume of right and left hippocampus was 2.90 cm3 and 2.78 cm3, respectively. On paired analys1s, this difference was significant The literature Indicated these volumes are in the m1ddle of a wide range of hippocampal volumes (1.73–5.68 cml) 1n both MRI‐based and histology‐based studies. Th1s wide variation can be expla1ned by differing hippocampal boundary definitions; technical factors of image processing, segmentation, and display; sample heterogeneity; and interoperator differences.
Latent infection membrane protein transmembrane FWLY is critical for intermolecular interaction, raft localization, and signaling

Relatively little is known about the biochemical mechanisms through which the Epstein–Barr virus latent infection integral membrane protein 1 (LMP1) transmembrane domains cause constitutive LMP1 aggregation and continuous cytoplasmic C terminus-mediated signal transduction. We now evaluate the role of the three consecutive LMP1 hydrophobic transmembrane pairs, transmembrane domains (TM)1-2, TM3-4, and TM5-6, in intermolecular aggregation and NF-κB activation. LMP1TM1-2 enabled ≈40% of wild-type LMP1 cytoplasmic domain-mediated NF-κB activation, whereas TM3-4 or TM5-6 assayed in parallel had almost no effect independent of LMP1TM1-2. Alanine mutagenesis of conserved residues in LMP1TM1-2 identified FWLY38–41 to be critical for LMP1TM1-2 intermolecular association with LMP1TM3-6. Further, in contrast to wild-type LMP1, LMP1 with FWLY38–41 mutated to AALA38–41 did not (i) significantly partition to lipid Rafts or Barges and effectively intermolecularly associate, (ii) enable cytoplasmic C terminus engagement of tumor necrosis factor receptor-associated factor 3, (iii) activate NF-κB, and thereby (iv) induce tumor necrosis factor receptor-associated factor 1 expression. Other LMP1 intermolecular associations were observed that involved LMP1TM1-2/LMP1TM1-2 or LMP1TM3-4/LMP1TM3-6 interactions; these probably also contribute to LMP1 aggregation. Because FWLY38–41 was essential for LMP1-mediated signal transduction, and LMP1 activation of NF-κB is essential for proliferating B lymphocyte survival, inhibition of LMP1FWLY41-mediated LMP1/LMP1 intermolecular interactions is an attractive therapeutic target.
Improved Retention for a Al2O3 IPD Embedded Flash Cell without Top-Oxide

Using a 2Mb embedded Flash cell array as a demonstrator, we reported previously that a 3 V reduction in programming voltage was possible by replacing the ONO inter- poly dielectric (IPD) with an IPD comprising the high-k material, AI2O3 by Kakoschke, R. , et al, (2007). Adding a thin protective top-oxide to the high-k IPD was later shown to significantly improve reliability in Power, J. R. , et al, (2007). In this paper, we show that for integration schemes more suited to the material properties of AI2O3, reliably functioning 2 Mb demonstrators with a high-k IPD but without top-oxide protection are also feasible.
Clinical, parasitological and obstetric observations in pregnant bitches with experimental toxoplasmosis

Eight pregnant mixed breed bitches, serologically negative for Toxoplasma gondii, were divided into three groups. Group I : bitches 01 and 02 (1.0 x 107 tachyzoites of Toxoplasma gondii, subcutaneous route); Group II: bitches 03, 04 and 05 (1.5 x 104 oocysts, oral route); Group III: bitches 06, 07 and 08 (as control). Clinical and obstetric examinations were conducted twice a day on each bitch, and weekly ultra-sonografic evaluations were performed until the end of pregnancy. From the 2nd day the bitches presented clinical alterations such as fever, nasal flowing, lachrymation, prostration, lymphadenopathy, premature parturition, abortion and fetal death. In Group I, Toxoplasma gondii was isolated from the bitches urine (day 10) and saliva (day 18). The bitches inoculated with oocysts showed milk samples collected on day 07 and day 16 after inoculation positive for Toxoplasma gondii. Saliva collected on day 13 and urine samples collected on days 1, 3, 7 and 16 from these bitches also showed positive. All inoculated bitches reacted positively to antigenic stimulus, with production of Toxoplasma gondii antibodies from day 3 on, with highest titers detected on day 11. Antibodies were detected by Indirect fluorescent antibody test (IFAT) and Enzyme linked immunosorbent assay (ELISA) tests.
Hyperactive behaviour in the mouse model of mucopolysaccharidosis IIIB in the open field and home cage environments

Mucopolysaccharidosis IIIB (MPS IIIB) is a lysosomal storage disorder characterized by severe behavioural disturbances and progressive loss of cognitive and motor function. There is no effective treatment, but behavioural testing is a valuable tool to assess neurodegeneration and the effect of novel therapies in mouse models of disease. Several groups have evaluated behaviour in this model, but the data are inconsistent, often conflicting with patient natural history. We hypothesize that this discrepancy could be due to differences in open field habituation and home cage behaviour. Eight‐month‐old wild‐type and MPS IIIB mice were tested in a 1‐h open field test, performed 1.5 h after lights on, and a 24‐h home cage behaviour test performed after 24 h of acclimatization. In the 1‐h test, MPS IIIB mice were hyperactive, with increased rapid exploratory behaviour and reduced immobility time. No differences in anxiety were seen. Over the course of the test, differences became more pronounced with maximal effects at 1 h. The 24‐hour home cage test was less reliable. There was evidence of increased hyperactivity in MPS IIIB mice, however, immobility was also increased, suggesting a level of inconsistency in this test. Performance of open field analysis within 1–2 h after lights on is probably critical to achieving maximal success as MPS IIIB mice have a peak in activity around this time. The open field test effectively identifies hyperactive behaviour in MPS IIIB mice and is a significant tool for evaluating effects of therapy on neurodegeneration.
A Generic Theory of the Integer Quantum Hall Effect

The integer quantum Hall effect (IQHE) is usually modeled by a Galilean or rotationally invariant Hamiltonian. These are not generic symmetries for electrons moving in a crystal background and can potentially confuse non-topological quantities with topological ones and identify otherwise distinct geometrical properties. In this thesis we present a generic theory for the IQHE. First we show that a generic guiding-center coherent state, defined by a natural metric in each Landau level, has the form of an antiholomorphic function times an exponential factor. Then by numerically solving the eigen problem for a quartic Hamiltonian and finding the roots of the antiholomorphic part we are able to define a topological spin sn = n + 1 2 where n is the number of central roots that are enclosed by the semiclassical orbit. We derive a generic formula for the Hall viscosity in the absence of rotational symmetry and show that the previous interpretation of the scalar Hall viscosity as the “intrinsic orbital angular momentum” breaks down since the concept of angular momentum requires the presence of rotational symmetry. We also calculate generic electromagnetic responses and differentiate between universal terms that are diagonal with respect to Landau level index and non-universal terms that depend on inter-Landau-level mixing. We conclude that the generic theory offers a fundamental definition for the topological spin and reveals finer structure in the geometrical properties of the IQHE.
Densest Local Structures of Uniaxial Ellipsoids

Connecting the collective behavior of disordered systems with local structure on the particle scale is an important challenge, for example in granular and glassy systems. Compounding complexity, in many scientific and industrial applications, particles are polydisperse, aspherical or even of varying shape. Here, we investigate a generalization of the classical kissing problem in order to understand the local building blocks of packings of aspherical grains. We numerically determine the densest local structures of uniaxial ellipsoids by minimizing the Set Voronoi cell volume around a given particle. Depending on the particle aspect ratio, different local structures are observed and classified by symmetry and Voronoi coordination number. In extended disordered packings of frictionless particles, knowledge of the densest structures allows to rescale the Voronoi volume distributions onto the single-parameter family of $k$-Gamma distributions. Moreover, we find that approximate icosahedral clusters are found in random packings, while the optimal local structures for more aspherical particles are not formed.