Miracle-dz/UniFAR-FTUs huggingface dataset:
Post-Traumatic Stress Disorder Symptomatology in People affected by Home Eviction in Spain

Abstract Despite the higher proportion of foreclosures and home evictions executed in Spain, compared to other countries, and the known link between social exclusion and mental health problems, studies exploring this association in Spain remain scarce. This study investigated the link between the process of home eviction and the appearance of symptomatology of post-traumatic stress disorder (PTSD), anxiety, depression, and perceived stress. Two hundred and five people affected by the process of home eviction were assessed using a structured interview that included three validated assessment instruments for PTSD, perceived stress, anxiety and depression. Analysis involved comparison with the normative groups that formed the validation studies together with regression analysis to determine the major psychological and socio-demographic predictors of perceived stress. Of the participants, 95.1% reported that they were experiencing the process of home eviction with fear, helplessness, or horror. In PTSD symptomatology, they scored higher than the normative PTSD group in symptoms of avoidance (t = 5.01; p < .05), activation (t = 5.48; p < .01), and total score (t = 4.15; p < .05). Of this subgroup, 72.5% fulfilled the DSM-IV symptom criteria for PTSD. The major predictor of perceived stress was PTSD symptomatology (B = .09; p < .001). The process of home eviction in Spain is having an alarming impact on mental health of affected people calling for effective measures to provide psychological and social support.
Receptor-mediated uptake and degradation of 125I-chemotactic peptide by human neutrophils.

iV-Formyl-Nle-Leu-Phe-Nle-Tyr-Lys has been synthesized and is shown to be a potent chemoattractant for human neutrophils. A concentration of 0.4 nM induces a chemotactic response which is 50% of the maximal value. Radioiodination yields a peptide with high spe- cific radioactivity (800 to 2000 Ci/mmol) and full biological activity. Binding of the ‘““I-formyl-peptide to human neutrophils at 24°C is highly specific, rapid, and saturable. Reversibility can be demonstrated immedi-ately following binding, but a time-and temperature- dependent process rapidly follows which alters the receptor*ligand complex so that dissociation no longer
The Neon Color Effect in the Ehrenstein Illusion

Van Tuijl's neon color effect arises in the Ehrenstein figure if a colored cross is added such as to connect the black arms across the central gap. The effect consists of a circular veil of color in the illusory area and has the same hue as the inducing cross. The neon-like coloration is uniform, or when elicited by two colors bipartite; it is strongest on backgrounds resembling the color of the cross. The effect cannot be attributed to chromatic aberration or eye movements. In foveal vision (and for red crosses) neon spreading is limited to gap sizes between 4 and 35 min of arc. Extrafoveally, gap sizes may be larger by a factor of two. Neon perception is enhanced by flicker and weakened if stimuli are oriented obliquely. It does not occur with dichoptic presentation. A maximum illusion requires that the Ehrenstein figure and cross are laterally and angularly aligned for good perceptual continuation. A neuronal origin by spreading and summation, together with cognitive processes, is proposed.
SLCO1B1 Polymorphisms and Statin-Induced Myopathy

Statin drugs are highly effective in lowering blood concentrations of LDL-cholesterol, with concomitant reduction in risk of major cardiovascular events. Although statins are generally regarded as safe and well-tolerated, some users develop muscle symptoms that are mostly mild but in rare cases can lead to life-threatening rhabdomyolysis. The SEARCH genome-wide association study, which has been independently replicated, found a significant association between the rs4149056 (c.521T>C) single-nucleotide polymorphism (SNP) in the SLCO1B1 gene, and myopathy in individuals taking 80 mg simvastatin per day, with an odds ratio of 4.5 per rs4149056 C allele. The purpose of this paper is to assemble evidence relating to the analytical validity, clinical validity and clinical utility of using SLCO1B1 rs4149056 genotyping to inform choice and dose of statin treatment, with the aim of minimising statin-induced myopathy and increasing adherence to therapy. Genotyping assays for the rs4149056 SNP appear to be robust and accurate, though direct evidence for the performance of array-based platforms in genotyping individual SNPs was not found. Using data from the SEARCH study, calculated values for the clinical sensitivity, specificity, positive- and negative-predictive values of a test for the C allele to predict definite or incipient myopathy during 5 years of 80 mg/day simvastatin use were 70.4%, 73.7%, 4.1% and 99.4% respectively. There is a need for studies comparing the clinical validity of SLCO1B1 rs4149056 genotyping with risk scores for myopathy based on other factors such as racial background, statin type and dose, gender, body mass index, co-medications and co-morbidities. No direct evidence was found for clinical utility of statin prescription guided by SLCO1B1 genotype.
Calcium dependence of villin-induced actin depolymerization.

"Cutting" of actin filaments by villin was evaluated from the time course of filament depolymerization. Depolymerization was initiated by diluting polymerized actin, labeled with a fluorescent probe on either lysine-374 or cysteine-375, to a concentration well below the critical into a medium containing free villin and various concentrations of calcium (in addition to potassium and magnesium). It was observed that at high calcium concentrations (200 microM) the time course of depolymerization could not be described by the single exponential that defines it at low calcium and low villin levels. Instead, at high calcium, the exponent increased with time and the rate of depolymerization became greater than that of controls in the absence of villin. This contrasts with the inhibition of depolymerization by villin at low calcium. The latter inhibition is a consequence of the capping of the barbed filament end by villin as are the inhibition of filament elongation and the elevation of the critical concentration. Evidence is presented that the effects of villin at high calcium are the result of cutting of the actin filaments by villin. It thus appears that different calcium binding sites control capping and cutting and that the calcium binding sites regulating cutting have a much lower affinity for calcium than the sites regulating capping of the barbed filament ends.
One-stage arthroscopically assisted anterior and posterior cruciate ligament reconstruction.

PURPOSE To retrospectively evaluate 15 consecutive patients who underwent simultaneous isolated, arthroscopically assisted anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) reconstruction. TYPE OF STUDY Case series. METHODS A bone-patellar tendon-bone autograft was used as the PCL substitute and doubled hamstring tendons were used as the ACL graft. The IKDC evaluation form and the HSS, Lysholm, and Tegner clinical rating scales were used to make clinical evaluations. Anteroposterior translation was measured with the KT-2000 arthrometer and stress view radiography. RESULTS At final IKDC evaluation, 3 patients (20%) were graded A, 7 (46.7%) were graded B, 3 (20%) were graded C, and 1 patient (6.7%) was graded D. One patient underwent revision surgery in another hospital for severe postoperative residual laxity. Two C-graded patients had an unsatisfactory outcome as a result of serious complications related to knee injuries. All patients with a grade A or B returned to sports activity. At stress view examination, mean posterior side-to-side translation measured at the lateral tibial plateau was 5.8 +/- 1.1 mm and the mean translation at the medial tibial plateau was 7.3 +/- 1.5 mm; the mean anterior dislocation was 3.3 +/- 0.4 mm. The preoperative HSS score rated an average of 32 +/- 9. Postoperatively, the average score reached was 89.6 +/- 8.3. The preoperative Lysholm score was 65.5 +/- 9.1 (range, 48 to 78) in patients with chronic lesions and at follow-up was 95.1 +/- 4.5 (range, 88 to 100). The average Tegner activity score decreased in patients with chronic lesions from 6.9 +/- 1.7 (range, 4 to 9) before injury to 5.5 +/- 1.6 (range, 2 to 9) at follow-up (P =.053 ). At follow-up, 7 patients (50%) returned to their preinjury level after surgery. CONCLUSIONS These results show the effectiveness and safety of simultaneous arthroscopic reconstruction of both cruciate ligaments using autografts that can adequately restore satisfactory stability, even in the presence of minimal peripheral laxity (1 degrees or 2 degrees ). We conclude that the use of autografts can restore ligament function with good patient compliance and without significant surgical complications.
Inhibition of antigen‐presenting cell function by alendronate in vitro

Bisphosphonates are potent inhibitors of bone resorption in vivo and are emerging as important and widely used drugs for the treatment of a variety of abnormal bone resorptive processes. In the current study we investigated the in vitro effects of 4‐amino‐1‐hydroxybutylidene‐1,1‐bisphosphonate (alendronate), a recently developed, extremely potent bisphosphonate, on the immune functions of human peripheral blood mononuclear cells (PBMCs). PBMC proliferation induced by lectins, alloantigens, and a nominal antigen (tetanus toxoid) was inhibited in a dose‐dependent manner by alendronate. Pretreatment of monocytes, but not T cells, with the compound at concentrations ranging from 10−4 to 10−8 M was inhibitory, indicating that alendronate acts selectively on antigen‐presenting cells (APCs). Alendronate did not affect the viability of monocytes or T cells or the expression of cell surface molecules known to play critical roles in antigen presentation. Alendronate exhibited dose‐dependent inhibition of the production of interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α) by activated monocytes. The inhibitory effect of 10−6 M alendronate on PBMC proliferation was reversed by 10 U/ml recombinant rIL‐1β, whereas other cytokines such as IL‐6, TNF‐α, and granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) had no effect. Thus, alendronate acts on monocytes to inhibit their antigen‐presenting/accessory cell functions through a mechanism that can be overcome by exogenous IL‐1. The inhibitory effect of this agent on cytokine production may contribute to its inhibitory effect on bone resorption.
Patterns of morphological integration in the dental arches of individuals with malocclusion

In humans, there is a large range of variation in the form of the maxillary and mandibular dental arches. This variation can manifest as either prognathism or retrognathism in either or both arches, which can cause malocclusion and lead to abnormal masticatory function. This study aims to identify aspects of variation and morphological integration existing in the dental arches of individuals with different types of malocclusion.
Absent auditory brain stem response: Peripheral hearing loss or brain stem dysfunction?

Interpretation of auditory brain stem response (ABR) findings can be problematic in cases where waves III and V are absent. Such findings can be attributed to profound hearing loss, brain stem neuropathology, or both. Over a 3‐year period, 48 patients with no known brain stem damage and on whom audiologic data were available were found to have no response by ABR or absent waves III and V. Severe to profound hearing loss was documented in 38 cases, audiometric data were equivocal in 3 cases, and 7 patients showed pure tone sensitivity ranging from normal hearing to moderate impairment. Thus 15% had better hearing sensitivity than might have been expected from their ABR findings. Each of these patients also exhibited abnormal acoustic reflex findings. We report the electrophysiological (ABR, MLR, acoustic reflex}, medical (history, neurological, EEG, CT scan) and behavioral (audiometric, speech and language, learning disabilities, psychological) data which characterize this group of patients.
Crimean-Congo haemorrhagic fever cases in Turkey

Crimean-Congo haemorrhagic fever (CCHF) is an arbovirus infection, which is transmitted through ticks or via blood and secretions. Until recently, human cases of CCHF were unknown in Turkey; however, several acute disease cases were reported in 2002. We report on the investigation of a cluster of suspected CCHF cases in the middle part of the Black Sea from May 2002 to October 2003. The medical charts that we reviewed were obtained from all local physicians and our field investigations. ‘Suspected case’ was defined with regard to time, place, and both clinical and laboratory characteristics. A total of 108 patients were defined as suspected case. Among them 36 patients were reached and blood samples taken for examination for CCHF by using ELISA and RT-PCR. According to the laboratory analysis, 80.6% (29/36) were acute cases and 8.3% (3/36) were past CCHF infections. The overall mortality rate was 5.6%. There was no nosocomial infection; however, there were 2 family clusters. Tick exposure was the most prevalent risk factor (74.2%). A multidisciplinary collaboration should be developed in order to understand the magnitude of the disease and also to keep it under control.
Neural mechanisms generating respiratory pattern in mammalian brain stem-spinal cord in vitro. I. Spatiotemporal patterns of motor and medullary neuron activity.

1. An analysis of the spatial and temporal patterns of activity of neurons of the respiratory motor-pattern generation system in an in vitro neonatal rat brain stem-spinal cord preparation is presented. Impulse discharge patterns of spinal and cranial moto-neurons as well as respiratory neurons in the medulla were analyzed. Patterns of motoneuronal discharge were characterized at the population level from recordings of motor-nerve discharge and at the single-cell level from intracellular recordings. These patterns were compared to patterns generated in the neonatal rat and adult mammal in vivo to establish the correspondence between in vitro and in vivo states. 2. The in vitro system generated a complex spatiotemporal pattern of spinal and cranial motoneuron activity during inspiratory (I) and expiratory (E) phases of the respiratory cycle. The respiratory cycle consisted of three distinct phases of neuronal activity (I, early E, and late E phase) similar to the temporal organization of the cycle in the intact mammal. The spike discharge pattern of motoneurons during the I phase consisted of a rapidly peaking-slowly decrementing discharge envelope with a high degree of synchronization on a time scale of 25-50 ms (approximately 20-40 Hz). A similar pattern was generated in the neonate in vivo under conditions comparable with the in vitro state (i.e., nervous system isolated from mechanosensory afferent inputs). However, the I-phase-motoneuron discharge pattern and cycle-phase durations differed from those characteristic of the intact neonatal or adult systems in vivo. This difference could be accounted for primarily by removal of vagal mechanosensory afferent inputs. 3. The synaptic drive potentials of spinal motoneurons during the I phase in vitro consisted of a rapidly peaking-slowly decrementing potential envelope similar in shape to the spike-frequency histogram of single motoneurons and the envelope of the motoneuron-population discharge. The drive potentials had prominent high-frequency amplitude fluctuations superimposed on the slower drive-potential envelope that were temporally correlated with the generation of motoneuron action potentials. The dominant frequency components of these fast-membrane-potential oscillations (20-35 Hz) were similar to the frequency components of the amplitude fluctuations in the motoneuron-population discharge. One class of medullary neurons with I-phase discharge also exhibited a rapidly peaking-slowly decrementing pattern of impulse discharge and synaptic drive potential with similar high-frequency components. (ABSTRACT TRUNCATED AT 400 WORDS)
Exploring a Cognitive Intervention for Children with Pervasive Developmental Disorder

Background. Pervasive developmental disorders (PDD) affect approximately 3–4% of the childhood population. PDD pervades every aspect of a child's life, having significant adverse effects on occupational performance. Purpose. This study investigates a new treatment approach to treating children with PDD, the Cognitive Orientation to Occupational Performance (CO-OP). CO-OP emphasizes problem-solving strategies and guided discovery of child- and task-specific strategies. Method. Three goals were established in collaboration with the parents and the child. Pre- and post-measures of parents' perceptions of child performance were identified using the Canadian Occupational Performance Measure (COPM). Repeated measures were taken using clinical observations, video analysis, and the Performance Quality Rating Scale (PQRS). Findings. Improved COPM ratings of performance and satisfaction were observed, and these results were paralleled by improved PQRS scores. Practice Implications. Self-report and observer report together provide preliminary evidence of the effectiveness of the CO-OP approach with children who have PDD supporting the use of CO-OP and suggesting further investigation.
Susceptibility to Insulin Resistance after Kidney Donation: A Pilot Observational Study

Background: In chronic kidney disease the contribution of decreased glomerular filtration rate (GFR) versus enhanced inflammation to cause insulin resistance (IR) is controversial. Aim: This pilot observational study examines, therefore, the prevalence of IR after kidney donation and factors that may determine its level. Methods: Insulin, proinsulin, adiponectin, malondialdehyde, and hsCRP were measured by conventional techniques in 14 previous kidney donors and 25 healthy volunteers. Results: Estimated GFR from Cockcroft-Gault formula of 76.42 ± 19.39 ml/min/1.73 m2 in the nephrectomized group was significantly lower (p < 0.01) than that in the control group of 125 ± 32.9 ml/min/1.73 m2. Fasting serum insulin of 16.57 ± 16.86 mU/l and homeostasis model assessment of insulin resistance (HOMA-IR) of 4.86 ± 5.11 in the nephrectomized group were significantly higher (p < 0.01) than the insulin level of 6.02 ± 4.06 mU/l and HOMA-IR of 1.5 ± 1.06 in the control group. There was no significant difference in levels in inflammatory mediators between the two groups. None of the tested inflammatory mediators correlated significantly with IR. Conclusion: Reduced GFR alone in previous kidney donors is associated with increased IR.
Selection and test of response measures in the study of the human newborn.

Unfortunately, Weiss's criterion of classification-the occurrence of "resemblances"-does not provide an unambiguous rule for ordering the behavior of newborns. Should we attend to resemblances in anatomical topography, or speed, or temporal overlap, or effect on the environment, or relation to antecedent stimulation? In fact, all of these criteria for classification have been used by students of neonatal behavior, with little evidence that a simple general rule for response definition is emerging. The problem of response definition is by no means confined to research with infants-it has long been an issue in general psychology (19, 22)-but the newborn presents special difficulties in answering this most basic of research questions: What are the effective response-classes? In the observation of animals and older human beings, psychologists of all theoretical commitments depend on movement in relation to the environment and on speech to provide the most elemental definition of response categories. Whether in the study of maze-learning or of psychotherapy, the
Purification and Characterization of Glutathione Reductase from Bovine Erythrocytes

Abstract Glutathione reductase (E.C.1.8.1.7; GR) was purified from bovine erythrocytes and some characteristics properties of the enzyme were investigated. The purification procedure was composed of preparation of the hemolysate, ammonium sulfate fractionation, affinity chromatography on 2′,5′-ADP Sepharose 4B, and gel filtration chromatography on Sephadex G-200. As a result of four consecutive procedures, the enzyme was purified 31,250-fold with a yield of 11.39%. Specific activity at the final step was 62.5 U (mg proteins)−1. For the enzyme, optimum pH, optimum temperature, optimum ionic strength, and stable pH were found to be 7.3, 55°C, 435 mM, 7.3, respectively. The molecular weight of the enzyme was found to be 118 kDa by Sephadex G-200 gel filtration chromatography and the subunit molecular weight was found to be 58 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In addition, KM and Vmax values were determined for glutathione disulfide (GSSG) and NADPH. Ki constants and inhibition types were established for glutathione (GSH) and NADP+. Also, effects of NADPH and GSSG were investigated on the enzyme activities.
Growth Hormone Antibodies Formation in Patients Treated with Recombinant Human Growth Hormone

Human growth hormone (hGH) is normally produced by acidophilic cells of the anterior lobe of the pituitary gland. Recombinant DNA technology has made it possible to produce rhGH. There have been reports of immunological reactions in patients treated with rhGH. For this reason, it is necessary to check sera of patients for presence of antibody against rhGH. Forty-seven children were treated for up to 6 months with recombinant human growth hormone (rhGH-Novo), 0.1 IU/Kg body weight, subcutaneously, three times weekly. The magnitude of growth response was similar to those expected from clinical experience with pituitary growth hormone. We examined sera for specific antibodies against rhGH by ELISA methods. Four patients developed serum antibodies against growth hormone. The analysis of these four sera by Dot blotting method also showed presence of antibodies against rhGH. In the sera of treated patients, pre-incubated with different concentration of rhGH, specific antibodies were detected by neutralizing assay. This finding was confirmed by ELISA technique. In conclusion, the main concern with anti-GH antibodies could be their ability to neutralize circulating growth hormone and inhibition its growth promoting effect.
Application of Siderotyping for Characterization of Pseudomonas tolaasii and “Pseudomonas reactans” Isolates Associated with Brown Blotch Disease of Cultivated Mushrooms

ABSTRACT Pyoverdine isoelectric focusing analysis and pyoverdine-mediated iron uptake were used as siderotyping methods to analyze a collection of 57 northern and central European isolates of P. tolaasiiand “P. reactans. ” The bacteria, isolated from cultivated Agaricus bisporus or Pleurotus ostreatus mushroom sporophores presenting brown blotch disease symptoms, were identified according to the white line test (W. C. Wong and T. F. Preece, J. Appl. Bacteriol. 47:401–407, 1979) and their pathogenicity towards A. bisporus and were grouped into siderovars according to the type of pyoverdine they produced. Seventeen P. tolaasii isolates were recognized, which divided into two siderovars, with the first one containing reference strains and isolates of various geographical origins while the second one contained Finnish isolates exclusively. The 40 “P. reactans” isolates divided into eight siderovars. Pyoverdine isoelectric focusing profiles and cross-uptake studies demonstrated an identity for some “P. reactans” isolates, with reference strains belonging to theP. fluorescens biovars II, III, or V. Thus, the easy and rapid methods of siderotyping proved to be reliable by supporting and strengthening previous taxonomical data. Moreover, two potentially novel pyoverdines characterizing one P. tolaasiisiderovar and one “P. reactans” siderovar were found.