Miracle-dz/UniFAR-FTUs huggingface dataset:
The Role of Autologous Fat Grafting in Secondary Microsurgical Breast Reconstruction

BackgroundAutologous breast reconstruction offers higher rates of patient satisfaction, but not all patients are ideal candidates, often due to inadequate volume of donor sites. Although autologous fat grafting is frequently used to augment volume and contour abnormalities in implant-based breast reconstruction, its clear utility in microsurgical breast reconstruction has yet to be defined. Here, we examined patients undergoing autologous microsurgical breast reconstruction with and without the adjunct of autologous fat grafting to clearly define utility and indications for use. MethodsA retrospective review of all patients undergoing autologous breast reconstruction with microvascular free flaps at a single institution between November 2007 and October 2011 was conducted. Patients were divided into 2 groups as follows: those requiring postoperative fat grafting and those not requiring fat grafting. Patient demographics, indications for surgery, history of radiation therapy, patient body mass index, mastectomy specimen weight, need for rib resection, flap weight, and complications were analyzed in comparison. ResultsTwo hundred twenty-eight patients underwent 374 microvascular free flaps for breast reconstruction. One hundred (26.7%) reconstructed breasts underwent postoperative fat grafting, with an average of 1.12 operative sessions. Fat was most commonly injected in the medial and superior medial poles of the breast and the average volume injected was 147.8 mL per breast (22–564 mL). The average ratio of fat injected to initial flap weight was 0.59 (0.07–1.39). Patients undergoing fat grafting were more likely to have had deep inferior epigastric perforator and profunda artery perforator flaps as compared to muscle-sparing transverse rectus abdominis myocutaneous. Patients additionally were more likely to have a prophylactic indication 58% (n = 58) versus 42% (n = 117) (P = 0.0087), rib resection 68% (n = 68) versus 54% (n = 148) (P < 0.0153), and acute postoperative complications requiring operative intervention 7% (n = 7) versus 2.1% (n = 8) (P < 0.0480). Additionally, patients undergoing autologous fat grafting had smaller body mass index, mastectomy weight, and flap weight. ConclusionsFat grafting is most commonly used in those breasts with rib harvest, deep inferior epigastric perforator flap reconstructions, and those with acute postoperative complications. It should be considered a powerful adjunct to improve aesthetic outcomes in volume-deficient autologous breast reconstructions and additionally optimize contour in volume-adequate breast reconstructions.
Inhibition of Risperidone Metabolism by Fluoxetine in Patients With Schizophrenia: A Clinically Relevant Pharmacokinetic Drug Interaction

The effect of fluoxetine on the steady-state plasma concentrations of risperidone and its active metabolite 9-hydroxyrisperidone (9-OH-risperidone) was evaluated in 10 patients with schizophrenia or schizoaffective disorder. Patients stabilized on risperidone (4–6 mg/day) received additional fluoxetine (20 mg/day) to treat concomitant depression. One patient dropped out after 1 week due to the occurrence of akathisia associated with markedly increased plasma risperidone concentrations. In the other subjects, mean plasma concentrations of risperidone increased during fluoxetine administration from 12 ± 9 ng/mL at baseline to 56 ± 31 at week 4 (p < 0.001), while the levels of 9-OH-risperidone were not significantly affected. After 4 weeks of combined treatment, the levels of the active moiety (sum of the concentrations of risperidone and 9-OH-risperidone) increased by 75% (range, 9–204%, p < 0.01) compared with baseline. The mean plasma risperidone/9-OH-risperidone ratio also increased significantly. During the second week of adjunctive therapy, two patients developed Parkinsonian symptoms, which were controlled with anticholinergic medication. These findings indicate that fluoxetine, a potent inhibitor of the cytochrome P450 enzyme CYP2D6 and a less potent inhibitor of CYP3A4, reduces the clearance of risperidone by inhibiting its 9-hydroxylation or alternative metabolic pathways. This interaction may lead to toxic plasma risperidone concentrations. In addition to careful clinical observation, monitoring plasma risperidone levels may be of value in patients given adjunctive therapy with fluoxetine.
Involvement of LEU13 in Interferon-Induced Refractoriness of Human RSa Cells to Cell Killing by X Rays

Abstract Kita, K. , Sugaya, S. , Zhai, L. , Wu, Y. P., Wano, C. , Chigira, S. , Nomura, J., Takahashi, S. , Ichinose, M. and Suzuki, N. Involvement of LEU13 in Interferon-Induced Refractoriness of Human RSa Cells to Cell Killing by X Rays. Radiat. Res. 160, 302–308 (2003). Culture of human cells with human interferon α and β (IFNA and IFNB) results in increased resistance of the cells to cell killing by X rays. To identify candidate genes responsible for the IFN-induced X-ray resistance, we searched for genes whose expression levels are increased in human RSa cells treated with IFNA, using an mRNA differential display method and Northern blotting analysis. RSa cells, which showed increased survival (assayed by colony formation) after X irradiation when they were treated with IFNA prior to irradiation, showed increased expression levels of LEU13 (IFITM1) mRNA after IFNA treatment alone. In contrast, IFr and F-IFr cells, both of which are derived from RSa cells, showed increased X-ray resistance and high constitutive LEU13 mRNA expression levels compared to the parental RSa cells. Furthermore, the IFNA-induced resistance of RSa cells to killing by X rays was suppressed by antisense oligonucleotides for LEU13 mRNA. LEU13, a leukocyte surface protein, was previously reported to mediate the actions of IFN such as inhibition of cell proliferation. The present results suggest a novel role of LEU13 different from that in the inhibition of cell proliferation, involved in IFNA-induced refractoriness of RSa cells to X rays.
The costs of recruiting apprentices: Evidence from German workplace-level data

In this article, we use workplace-level data to analyse the costs of filling an apprenticeship vacancy in Germany. We find that such recruitment costs amount on average to €600 per hire (almost one month’s pay of an apprentice or approximately 1–2 % of a workplace’s training expenditures), but costs are heterogeneous across workplaces and vary strongly by training occupation. Our results suggest that a high degree of competition among training workplaces in the region is associated with an increase in recruitment costs. Furthermore, we find that workplaces with a works council or an investment-oriented training strategy incur higher recruitment costs. Our results are important in light of the increasing competition for talented school leavers induced by demographic change.
FACILITATING RADICAL INNOVATION IN CONSUMER ELECTRONICS AND INFORMATION TECHNOLOGY INDUSTRIES

by SHENGFA JOHNSON CHUANG Radical innovation (RI) is widely recognized as a source of competitive advantage because it contributes to business sustainability and growth. This applies especially to firms operating in the hyper-competitive environment within consumer electronics (CE) and information technology (IT) industries. A recent Boston Consulting Group survey suggests that radical product innovation is one of three top strategic priorities for 71% of companies. Despite its importance, RI has been widely characterized as lacking order and an outcome of serendipity. Indeed, prior literature has largely ignored antecedents of RI or has studied them in isolation. This dissertation explores the interplay of RI antecedents in the context of CE/IT industries. I conducted an exploratory mixed-methods study on the nature and antecedents of RI in the CE/IT industries. The dissertation consists of three empirical studies. In the first study, I interview 38 product managers and engineers in the CE/IT industries to define the nature of RI and to identify challenges associated with RI. I found that RI emerges from identifying unarticulated market needs and matching them with new technological opportunities. In the second study, I conducted a survey among 408 innovation managers from CE/IT industries to
Deriphat 2‐DE to visualize polyphenol oxidase in Moscato and Prosecco grapes

The Deriphat 2‐DE was used to visualize polyphenol oxidase (PPO) isoforms of Moscato and Prosecco grape extracts, partially purified and characterized. Catecholase has similar values in the two varieties, whereas Moscato cresolase data are almost 54% higher. In the first dimension, the PPO of both varieties may be detected by SDS‐PAGE, but native PAGE (N‐PAGE) gave negative results. For this reason, the samples were solubilized in the zwitteronic detergent Deriphat, which was also included in the gel and the cathodic buffer. Deriphat migrated together with the cathodic buffer, maintaining protein solubility and revealing the PPO profiles of Moscato and Prosecco extracts in native conditions. The combination of Deriphat‐PAGE (D‐PAGE) and SDS‐PAGE (2‐DE) also resulted in improved separation efficiency in resolving PPO and specialized stains in evaluating PPO activities. The control, represented by IEF for the first‐dimensional separation, had a lower number of spots, demonstrating the higher capacity of Deriphat 2‐DE to isolate PPO isoforms from grape extracts. The Deriphat 2‐DE method described here is simple but powerful, and the resulting information will be a useful tool for further proteomic research.
Two- and Three-Dimensional Relationships Between Knee and Hip Kinematic Motion Analysis: Single-Leg Drop-Jump Landings.

CONTEXT Hip- and knee-joint kinematics during drop landings are relevant to lower-extremity injury mechanisms. In clinical research the "gold standard" for joint kinematic assessment is 3-dimensional (3D) motion analysis. However, 2-dimensional (2D) kinematic analysis is an objective and feasible alternative. OBJECTIVE To quantify the relationship between 2D and 3D hip and knee kinematics in single-leg drop landings and test for a set of 3D hip and knee kinematics that best predicts 2D kinematic measures during single-leg drop landings Design: Descriptive, comparative laboratory study. PARTICIPANTS 31 healthy college-age women (65.5 kg [SD 12.3], 168.1 cm [SD 6.7]). METHODS Participants performed five 40-cm single-leg landings during motion capture at 240 Hz. Multiple regressions were used to predict relationships for knee and hip between 2D frontal-plane projection angles (FPPA) and 3D measurements. RESULTS 2D knee FPPA had a strong relationship with 3D frontal-plane knee kinematics at initial contact (IC) (r2 = .72), which was only minimally improved with the addition of knee sagittal-plane and hip transverse-plane positions at IC (r2 = .77). In contrast, 2D knee FPPA had a low relationship with 3D knee-abduction excursion (r2 = .06). The addition of knee sagittal-plane and hip transverse-plane motions did not improve this relationship (r2 = .14). 2D hip FPPA had a moderate relationship with 3D frontal-plane hip position at IC (r2 = .52), which was strengthened with the addition of hip sagittal-plane position (r2 = .60). In addition, hip 2D FPPA into adduction excursion had a strong association with 3D hip-adduction excursion (r2 = .70). CONCLUSION 2D kinematics can predict 3D frontal-plane hip and knee position at IC during a single-leg landing but predict 3D frontal-plane knee excursion with far less accuracy.
Value investing with rule-based stock selection and data mining

Paradigm shift is on the way in the financial market and economics theory. Evidence against the previously prevailing assumptions of rationality and market efficiency has become abundant and new models, that are rapidly becoming the main stream, are based on the actual investor behavior that can be empirically observed and provide better fit to the data. The contrast between the old and the new schools of thought serves as a background for this study, and reviewing some contemporary theories and studies of topics such as enhanced value investing and contextual fundamental analysis justifies its results which under the efficient market hypothesis would be anomalous. The first objective of this study is to test in the Nordic markets (Finland, Sweden, Norway and Denmark) Joel Greenblatt’s investment formula (GF) that he published in his 2005 book: “The Little Book that Beats the Market”. His method aims to buy good stocks when they are cheap and has provided during period 1988-2004 an average 30.8% p.a. return in the U.S. stock market while the S&P 500 index yielded 12.4% annually. Although the average return performance is stellar, the selection rule also accepts some stocks that will be deeply in loss thus making the whole portfolio to suffer periods of underperformance. This undermines the formula’s exploitability especially in professional fund management setting. Second objective in this study is to improve GF by developing a model that can filter out the loss producing stocks ex ante using information set that is available at the time of investment. To accomplish the first goal a program that simulates GF investment rule was developed and run with the result of 29.4% p.a net investment return after taxes and trading costs during the research period 2000-2011 while the relevant reference stock index (FTSE Nordic Value Index) returned 7.6% p.a. To develop a model for the second stage, data mining methods were applied in variable and model selection phase. The produced logistic regression model with constant and 8 additional terms (single variables and interactions) was able to predict with 80-90% accuracy the predefined holding period return category for the cases in the research data. Sample data was divided into training, testing and validation partitions to ensure the out-of-sample performance of the models. When this model was applied as a filter in the stock selection phase the annual return for the period increased to 43.8 % p.a.
Identification of chromosome locations of genes affecting preharvest sprouting and seed dormancy using chromosome substitution lines in tetraploid wheat (Triticum turgidum L.).

Seed dormancy, the main factor contributing to preharvest sprouting (PHS) resistance, is a complex trait and is strongly infl uenced by environmental growth conditions. In this study, three sets of single chromosome substitution lines, including 36 genotypes, in a durum wheat [Triticum turgidum L. ssp. durum (Desf.) Husn. ] background with donor chromosomes originating from three wild emmer [T. turgidum L. ssp. dicoccoides (Korn. ex Asch. and Graebn.) Thell. ] accessions were grown in nine fi eld environments and evaluated for seed dormancy and PHS resistance. The substitution lines involving chromosome 3A were among the most dormant genotypes. Germination tests indicated that fi ve chromosomes contained genes infl uencing seed dormancy at a level comparable to 3A. Results from PHS tests showed that PHS was affected by at least eight T. dicoccoides chromosomes including 3A. The chromosomes harboring genes for seed dormancy did not fully correspond with those for PHS resistance. The weak correlations between PHS and dormancy observed in this study indicate that different genes are affecting these traits and they may be differentially infl uenced by the environment. Nonetheless, our results revealed that genes present on fi ve chromosomes, 2A, 2B, 3A, 4A, and 7B, were found to affect both PHS resistance and seed dormancy. These genotypes thus provide useful resources for further studies on genetic interactions that contribute to the overall phenotypic variation and on genetic dissection of quantitative trait loci underlying PHS resistance.
Rice Homeodomain Protein WOX11 Recruits a Histone Acetyltransferase Complex to Establish Programs of Cell Proliferation of Crown Root Meristem[OPEN]

Rice WOX11 promotes crown root development by interacting with a histone acetylation complex to induce permissive chromatin of gene expression. Shoot-borne crown roots are the major root system in cereals. Previous work has shown that the Wuschel-related homeobox gene WOX11 is necessary and sufficient to promote rice (Oryza sativa) crown root emergence and elongation. Here, we show that WOX11 recruits the ADA2-GCN5 histone acetyltransferase module to activate downstream target genes in crown root meristem. Rice ADA2 and GCN5 genes are highly expressed in root meristem and are shown to be essential for cell division and growth. WOX11 and ADA2-GCN5 commonly target and regulate a set of root-specific genes involved in energy metabolism, cell wall biosynthesis, and hormone response, some of which are known to be important for root development. The results indicate that the recruitment of ADA2-GCN5 by WOX11 establishes gene expression programs of crown root meristem cell division and suggest that permissive chromatin modification involving histone acetylation is a strategy for WOX11 to stimulate root meristem development.
The effect of political risk on emerging and developed markets

This thesis consists of three substantive chapters (3, 4, 5) on the impact of political risk on equity and exchange rate returns and their volatilities. Chapter 3 proposes a framework for predicting market returns and volatility using changes in the country’s political risk. We identify the appropriate lag to to calculate changes over, and how the changes should be included in mean and volatility equations. The level of aggregation of political risk variable is also examined. Analysing 47 emerging and 21 developed markets, we find predictive power primarily for volatility of emerging markets, and recommended use of three political risk components which suitably capture important dimensions of political environment. In the Chapter 4 we empirically examines the impact of political risk on returns and volatility of individual firms and industry portfolios from New Zealand and Pakistan. The data used in the study consist of 184 firms from New Zealand and 202 firms from Pakistan along with country-level political risk data from the ICRG. As in the , we find in Chapter 3 that the impact of political risk is more on volatility than the returns of firms in both markets. As we expect, the impact of political risk is more on Pakistani firms compared to those in New Zealand. Overall, results from the industry portfolios are according to the hypothesis that political risk impact is different across industries (volatility increase for some industries and decrease for few). Chapter 5 examine the relationship between political risk variables on the nominal exchange rate return and its volatility. We again investigate developed versus developed markets, and also consider three different exchange rate regimes i.e. floating, managed floating and fixed. This is important to examine the link between political risk and exchange rate because there are two sources of political risk one on either side of the exchange rate. In our analysis, we use the political risk spread between the country of interest and the USA. Overall results reveal that emerging markets are more exposed to political risk compared to developed. Further, the impact of political risk variables is more on the floating exchange rate compared to managed floating and fixed exchange rate as might be expected, since intervention in the market will generally reduce to eliminate the influence of alternative factors. We also find strong evidence that volatility increases more during a period of high political risk and poor economic conditions for emerging markets.
Extrachromosomal transposition of the transposable element Minos occurs in embryos of the silkworm Bombyx mori

To assess the ability of the transposable element Minos to act as a vector for genetic manipulation of the silkworm Bombyx mori, an extrachromosomal transposition assay based on three plasmids was performed. The three plasmids – helper, donor and target – were co‐injected into preblastoderm embryos. Low molecular weight DNA was extracted from the embryos at the stage of blastokinesis and used to transform Escherichia coli. High frequency of transposition was observed in the presence of a helper plasmid possessing an intronless Minos transposase gene, whereas transposition did not occur in the presence of a helper plasmid with the intron‐bearing transposase gene. Sequence analysis of the insertion sites showed that Minos always inserts into a TA dinucleotide. Although the insertions are distributed throughout the target gene, there was a preference for certain insertion sites. However, no consensus could be identified in the sequence flanking the target site. The results strongly suggest that the transposable element Minos has the potential to be used as a vector in the silkworm and probably in other lepidopteran insects.
Towards enterprise application integration principles for facility management software in hospitals

Currently, hospitals have often installed up to 100 software applications within the non-medical support services (FM). The applications are mostly un-coordinated and only accessible for employees of certain sub-areas. Therefore, data is often stored redundantly, has to be transferred manually and some stakeholders are unaware of data locations and/or cannot access the data. In order to become more efficient, to save resources and to increase (data) quality, hospitals are now forced to find solutions in their processes and IT architecture in the area of FM as well. Enterprise Application Integration (EAI) is an approach which has been applied to overcome this problem in other industries and partially also in the medical context in hospitals, but so far not including FM. During the latest research in non-medical support services in hospitals, connections and information needs between the different FM disciplines in hospitals became clearer. Extending these findings with the EAI principles in an explorative approach, the basis for future systematic integration of FM applications in hospitals is presented in this paper.
Detection of stratified microbial populations related to Chlorobium and Fibrobacter species in the Atlantic and Pacific oceans

A gene lineage (SAR406) related to Chlorobium and Fibrobacter species was found in 16S rRNA gene clone libraries prepared from samples from two oceans. The clone libraries were constructed from total picoplankton genomic DNA to assess bacterial diversity in the lower surface layer. The samples were collected by filtration from a depth of 80 m at a site in the western Sargasso Sea and from a depth of 120 m at a site in the Pacific Ocean, approximately 70 km from the Oregon coast. The PCR and primers which amplified nearly full-length 16S rRNA genes were used to prepare the clone libraries. Among the diverse gene clones in these libraries were two related clones (SAR406 and OCS307) which could not be assigned to any of the major bacterial phyla. Phylogenetic analyses demonstrated that these genes were distant relatives of the genus Fibrobacter and the green sulfur bacterial phylum, which includes the genus Chlorobium. The inclusion of SAR406 in phylogenetic trees inferred by several methods resulted in support from bootstrap replicates for the conclusion that Fibrobacter and Chlorobium species and SAR406 are a monophyletic group. An oligonucleotide probe that selectively hybridized to clone SAR406 was used to examine the distribution of this gene lineage in vertical profiles from the Atlantic and Pacific Oceans and in monthly time series at 0 and 200 m in the Atlantic Ocean. During stratified periods, the genes were most abundant slightly below the deep chlorophyll layer. Seasonal changes in the surface abundance of SAR406 rDNA were highly correlated with chlorophyll a levels (r = 0.75).