Miracle-dz/UniFAR-FTUs huggingface dataset:
Toscana Virus NSs Protein Inhibits the Induction of Type I Interferon by Interacting with RIG-I

ABSTRACT Toscana virus (TOSV) is a phlebovirus, of the Bunyaviridae family, that is responsible for central nervous system (CNS) injury in humans. Previous data have shown that the TOSV NSs protein is a gamma interferon (IFN-β) antagonist when transiently overexpressed in mammalian cells, inhibiting IRF-3 induction (G. Gori Savellini, F. Weber, C. Terrosi, M. Habjan, B. Martorelli, and M. G. Cusi, J. Gen. Virol. 92:71–79, 2011). In this study, we investigated whether an upstream sensor, which has a role in the signaling cascade leading to the production of type I IFN, was involved. We found a significant decrease in RIG-I protein levels in cells overexpressing TOSV NSs, suggesting that the nonstructural protein interacts with RIG-I and targets it for proteasomal degradation. In fact, the MG-132 proteasome inhibitor was able to restore IFN-β promoter activation in cells expressing NSs, demonstrating the existence of an evasion mechanism based on inhibition of the RIG-I sensor. Furthermore, a C-terminal truncated NSs protein (ΔNSs), although able to interact with RIG-I, did not affect the RIG-I-mediated IFN-β promoter activation, suggesting that the NSs domains responsible for RIG-I-mediated signaling and interaction with RIG-I are mapped on different regions. These results contribute to identify a novel mechanism for bunyaviruses by which TOSV NSs counteracts the early IFN response.
Hsp40 Interacts Directly with the Native State of the Yeast Prion Protein Ure2 and Inhibits Formation of Amyloid-like Fibrils*

Ure2 is the protein determinant of the [URE3] prion phenotype in Saccharomyces cerevisiae and consists of a flexible N-terminal prion-determining domain and a globular C-terminal glutathione transferase-like domain. Overexpression of the type I Hsp40 member Ydj1 in yeast cells has been found to result in the loss of [URE3]. However, the mechanism of prion curing by Ydj1 remains unclear. Here we tested the effect of overexpression of Hsp40 members Ydj1, Sis1, and Apj1 and also Hsp70 co-chaperones Cpr7, Cns1, Sti1, and Fes1 in vivo and found that only Ydj1 showed a strong curing effect on [URE3]. We also investigated the interaction of Ydj1 with Ure2 in vitro. We found that Ydj1 was able to suppress formation of amyloid-like fibrils of Ure2 by delaying the process of fibril formation, as monitored by thioflavin T binding and atomic force microscopy imaging. Controls using bovine serum albumin, Sis1, or the human Hsp40 homologues Hdj1 or Hdj2 showed no significant inhibitory effect. Ydj1 was only effective when added during the lag phase of fibril formation, suggesting that it interacts with Ure2 at an early stage in fibril formation and delays the nucleation process. Using surface plasmon resonance and size exclusion chromatography, we demonstrated a direct interaction between Ydj1 and both wild type and N-terminally truncated Ure2. In contrast, Hdj2, which did not suppress fibril formation, did not show this interaction. The results suggest that Ydj1 inhibits Ure2 fibril formation by binding to the native state of Ure2, thus delaying the onset of oligomerization.
Why Markets Tolerate Mediocre Manufacturing

Government pet the comeback of U.S. manufacturing. Bolstered by foreign direct investment, the current recovery was, until late 1995, the most investment-led in decades. Even when you include data revisions illustrating that manufacturing output bounced back less well in the 1990s than was previously believed, the rate of productivity advance in manufacturing has rebounded.1 But the sector's output has not grown enough to stem the continued erosion in its share of the
From Invention Success to Commercialization Success: Technology Ventures and the Benefits of Upstream and Downstream Supply‐Chain Alliances

An entrepreneurial venture's supply‐chain partnerships involve upstream alliances (with research universities) and downstream alliances (with large industry incumbents). Even though such partnerships bring the venture many benefits, they also come with significant challenges, notably the need to seamlessly combine a “technology push” philosophy with a “market pull” one. Utilizing a data set of over both upstream and downstream alliance partnerships spanning 603 technology ventures in the biotech industry, interesting results were found with regard to the impact of these two alliance partnerships on the venture's invention success and commercialization success. Upstream partnerships demonstrate a positive impact on invention success but no significant impact on commercialization success. However, with the moderating role of downstream partnerships, the results change: the joint effect of these two types of alliance partnerships is positive for both invention and commercialization success. The study findings have important implications with regard to the role and impact of alliance partnerships at the intersection of small business, entrepreneurship and technology innovation, and for researchers, practitioners, and policy makers.
The analysis of meat and bone traits of Plymouth Rock cockerels and capons (P55) at different age

ABSTRACT The aim of the study was the comparison of Plymouth Rock cockerels and capons and the evaluation of the influence of their age on slaughter yield, meat quality, and bone traits. The studies were conducted on cockerels and capons from P55 strain (Plymouth Rock). At 16, 18, and 20 wk of rearing, a dissection of whole carcasses of cockerels and capons was conducted, and breast and leg muscles were evaluated in terms of the physical and chemical traits, as well as the chemical composition. Also, traits of femur and tibia bones were studied. It was indicated that capons were characterized by a higher body weight (BW) before slaughter and higher weight of the eviscerated carcass than cockerels at consecutive dates of slaughter. Based on BW, the slaughter yield and muscle content of carcasses, it was found that the most optimal slaughter time in Plymouth Rock cockerels is at 18 wk and capons at 20 wk. It was observed that caponization had a significant effect on the chemical composition of breast muscles with respect to water and protein content. Along with bird age, collagen content in muscles decreased; however, significant differences were observed only in capons. At consecutive dates of evaluating cockerels, resistance and toughness of their femur and tibia bones increased significantly. Significant changes in the physical characteristics of bones were also found.
Stapled versus handsewn methods for colorectal anastomosis surgery: a systematic review of randomized controlled trials.

CONTEXT The interest in the results from comparisons between handsewing and stapling in colorectal anastomoses has been reflected in the progressive increase in the number of clinical trials. These studies, however, do not permit conclusions to be drawn, given the lack of statistical power of the samples analyzed. OBJECTIVE To compare stapling and handsewing in colorectal anastomosis, testing the hypothesis that in colorectal anastomosis the technique of stapling is superior to that of handsewing. DESIGN A systematic review of randomized controlled trials. SURVEY STRATEGY: Systematic revision of the literature and meta-analysis were used, without restrictions on language, dates or other considerations. The sources of information used were Embase, Lilacs, Medline, Cochrane Controlled Clinical Trials Database, and letters to authors and industrial producers of staples and thread. SELECTION CRITERIA Studies were included in accordance with randomization criteria. The external validity of the studies was investigated via the characteristics of the participants, the interventions and the variables analyzed. An independent selection of clinical studies focusing on analysis of adult patients attended to on an elective basis was made by two reviewers. DATA COLLECTION AND ANALYSIS The methodological quality of the studies was assessed by the same reviewers. In addition to the randomization criteria, the masking, treatment intention, losses and exclusions were also analyzed. The meta-analysis was performed using risk difference and weighted average difference, with their respective 95% confidence intervals. The variables studied were mortality, clinical and radiological anastomotic dehiscence, anastomotic stricture, hemorrhage, reoperation, wound infection, time taken to perform the anastomosis and hospital stay. RESULTS Nine clinical trials were selected. After verifying that it was possible to perform one of the two techniques being compared, 1,233 patients were included, of whom 622 underwent stapling and 611 the handsewing technique. No statistical difference was found between the variables, except for stenosis, which was more frequent in stapling (p < 0.05), and the time taken to perform the anastomosis, which was greater in handsewing (p < 0.05). CONCLUSION The evidence found was insufficient to demonstrate superiority of the stapling method over handsewing, independent of the level of colorectal anastomosis.
Distance to a Plastic Surgeon and Type of Insurance Plan Are Independently Predictive of Postmastectomy Breast Reconstruction

Background: The psychosocial benefits of postmastectomy breast reconstruction are well established; however, health care barriers persist. The authors evaluated statewide patient population to further identify obstacles to reconstruction. Methods: A linked data set combining the North Carolina Central Cancer Registry with administrative claims from Medicare, Medicaid, and private insurance plans identified women diagnosed with breast cancer from 2003 to 2006. For inclusion in the study, women must have had a mastectomy within 6 months of diagnosis and had continuous insurance enrollment at least 2 years postoperatively (n = 5381). Multivariable logistic regression was used to model odds of reconstruction. Results: Approximately 20 percent underwent reconstruction (n = 1130). Distance to a plastic surgeon—10 to 20 miles (OR, 0.78) and greater than 20 miles (OR, 0.73; p < 0.05)—was significantly predictive of no reconstruction, independent of other well-known disparities, including age, race, rural location, and lower household income. Women with government-funded health care, such as Medicare (OR, 0.58) and Medicaid (OR, 0.24; p < 0.001), were also significantly less likely to undergo reconstruction. Consistent with previous study, advanced cancer stage and receipt of radiation therapy decreased the likelihood of reconstruction. Furthermore, when the authors compared immediate to delayed reconstruction, rural location, chemotherapy, and radiation therapy were significantly predictive of delay. Conclusions: This is the first population-based study to demonstrate distance to care and insurance plan as significant predictors of receipt of reconstruction. Additional research is needed to understand health care barriers and to determine whether distance to a plastic surgeon can be ameliorated by outreach programs. CLINICAL QUESTION/LEVEL OF EVIDENCE: Risk, III.
BLEEDING ANGIODYSPLASIA: SHOULD WE CONCENTRATE MORE ON THE AORTIC VALVE THAN ON THE BOWEL?

A case of bleeding from angiodysplasia in association with aortic stenosis is presented. The particular interest in this patient is that the bleeding ceased immediately after the insertion of a bioprosthetic valve and recurred when the valve restenosed. Furthermore, the bleeding again promptly resolved when the valve was exchanged for a metallic prosthesis despite long‐term anticoagulation. The implications of these observations are discussed.
Inducible nitric oxide synthase is expressed in synovial fluid granulocytes

The objective of the study was to evaluate the NO‐producing potential of synovial fluid (SF) cells. SF from 15 patients with arthritis was compared with blood from the same individuals and with blood from 10 healthy controls. Cellular expression of inducible nitric oxide synthase (iNOS) was analysed by flow cytometry. High‐performance liquid chromatography was used to measure l‐arginine and l‐citrulline. Nitrite and nitrate were measured colourimetrically utilizing the Griess’ reaction. Compared to whole blood granulocytes in patients with chronic arthritis, a prominent iNOS expression was observed in SF granulocytes (P < 0·001). A slight, but statistically significant, increase in iNOS expression was also recorded in lymphocytes and monocytes from SF. l‐arginine was elevated in SF compared to serum (257 ± 78 versus 176 ± 65 µmol/l, P = 0·008), whereas a slight increase in l‐citrulline (33 ± 11 versus 26 ± 9 µmol/l), did not reach statistical significance. Great variations but no significant differences were observed comparing serum and SF levels of nitrite and nitrate, respectively, although the sum of nitrite and nitrate tended to be elevated in SF (19·2 ± 20·7 versus 8·6 ± 6·5 µmol/l, P = 0·054). Synovial fluid leucocytes, in particular granulocytes, express iNOS and may thus contribute to intra‐articular NO production in arthritis.
Lifetime prevalence of DSM-III-R psychiatric disorders among urban and rural Mexican Americans in California.

BACKGROUND The Mexican American Prevalence and Services Survey presents lifetime prevalence rates for 12 DSM-III-R psychiatric disorders in a sample of 3012 adults of Mexican origin by place of residence and nativity, and compares these results with those of population surveys conducted in the United States and Mexico. METHODS The stratified random sample included non-institutionalized persons aged 18 to 59 years of Mexican origin, who were residents of Fresno County, California. Psychiatric disorders were assessed using a modified version of the World Health Organization Composite International Diagnostic Interview in face-to-face interviews. RESULTS Mexican immigrants had lifetime rates similar to those of Mexican citizens, while rates for Mexican Americans were similar to those of the national population of the United States. This difference is attributable to a prevalence rate for any disorder among immigrants of 24.9%, compared with 48.1% among US-born respondents. A higher prevalence for any disorder was reported in urban (35.7%) compared with town (32.1%) or rural (29.8%) areas. Multivariate analyses showed an adjusted effect of country of birth, but not of urban residence. CONCLUSIONS Despite very low education and income levels, Mexican Americans had lower rates of lifetime psychiatric disorders compared with rates reported for the US population by the National Comorbidity Survey. Psychiatric morbidity among Mexican Americans is primarily influenced by cultural variance rather than socioeconomic status or urban vs rural residence.
The Effect of SPAM Administration During a Dynamic Simulation

Objective: The objective of this study was to determine whether administration of the situation present assessment method (SPAM) affects workload and/or task performance. Background: SPAM probes are thought to isolate workload from the assessment of situation awareness (SA) by including a warning signal before asking an SA-related question. However, there is a good reason to think that SPAM may still have an effect on dynamic workload and task performance. Specifically, nearly all dual tasks affect workload and performance; thus, it would be surprising if SPAM did not. Method: Following the methods of Durso, Bleckley, and Dattel, I evaluated workload and performance on the Air Traffic Scenarios Test in SPAM, non-SPAM probe, and no-probe conditions. Results: Global workload was unaffected by probe administration. However, at least with novice performers, SPAM probes affected task performance. Conclusion: The use of a warning signal does not eliminate performance decrements associated with secondary tasks. Moreover, there may be performance decrements unique to SPAM. Application: Cautious users of online assessment measures, such as SPAM, may want to remove performance immediately subsequent to probes from analysis, use an interprobe interval longer than 2.83 min, and construct their assessment measures to reduce display search times unrelated to primary task performance.
Preparticipation evaluation of novice, middle-age, long-distance runners.

PURPOSE The purpose of this study was to assess the cardiovascular health and risk profile in middle-age men making an entry to participate for their first time in a long-distance race. METHODS Male first-time participants, 45 yr and older, in the world's largest cross-country running race, the Lidingöloppet, were evaluated with a medical history and physical examination, European systematic coronary risk evaluation (SCORE), 12-lead ECG, echocardiography, and blood tests. Further diagnostic workup was performed when clinically indicated. RESULTS Of 265 eligible runners, 153 (58%, age 51 ± 5 yr) completed the study. Although the 10-yr fatal cardiovascular event risk was low (SCORE, 1%; interquartile range, 0%-1%), mild abnormalities were common, for example, elevated blood pressure (19%), left ventricular hypertrophy (6%), and elevated LDL cholesterol (5%). ECG changes compatible with the "athlete's heart" were present in 82%, for example, sinus bradycardia (61%) and/or early repolarization (32%). ECG changes considered training unrelated were found in 24%, for example, prolonged QTc-interval (13%), left axis deviation (5.3%), and left atrial enlargement (4%). In 14 runners (9%), additional diagnostic workup was clinically motivated, and 4 runners (2%) were ultimately discouraged from vigorous exercise because of QTc intervals >500 ms (n = 2), symptomatic atrioventricular block (n = 1), and cardiac tumor (n = 1). The physician examination and the ECG identified 12 of the 14 participants requiring further evaluation. CONCLUSIONS Cardiovascular evaluation of middle-age men, including a physician examination and a 12-lead ECG, appears useful to identify individuals requiring further testing before vigorous exercise. The additional yield of routine echocardiography was small.
Measurement-Based Hybrid Approach for Ringdown Analysis of Power Systems

System identification methods have been widely used for the study of low frequency electromechanical oscillations and the development of low order dynamic models. This paper introduces a hybrid frequency/time-domain approach to estimate the dominant modes contained in ringdown responses of power systems. Practical issues and solutions encountered in the application of the hybrid method are discussed. The performance of the proposed technique is evaluated by applying the Monte Carlo method to synthetic signals and simulated responses from a large-scale power system, as well as to measurements recorded in a microgrid laboratory test facility. Results in all cases proved to be very accurate, verifying the robustness of the proposed method.
Glycocalyx degradation leads to blood–brain barrier dysfunction and brain edema after asphyxia cardiac arrest in rats

The role of glycocalyx in blood–brain barrier (BBB) integrity and brain damage is poorly understood. Our study aimed to investigate the impacts of endothelial glycocalyx on BBB function in a rat model of cardiac arrest (CA) and cardiopulmonary resuscitation (CPR). Male Sprague-Dawley rats subjected to 8-min asphyxia CA/CPR. Compared to controls, glycocalyx was mildly injured by CA, severely disrupted by hyaluronidase (HAase) with CA, and mitigated by hydrocortisone (HC) with CA. More importantly, the disruption of glycocalyx caused by HAase treatment was associated with higher BBB permeability and aggravated brain edema at 24 h after return of spontaneous circulation, as well as lower survival rate and poorer neurologic outcome at seventh day. Reversely, less degradation of glycocalyx by HC treatment was accompanied by higher seven-day survival rate and better neurologic outcome. Mechanistically, HAase treatment further increased CA/CPR-induced activation of glia cells and expression of inflammatory factors, whereas HC decreased them in the brain cortex and hippocampus. Glycocalyx degradation results in BBB leakage, brain edema, and deteriorates neurologic outcome after asphyxia CA/CPR in rats. Preservation of glycocalyx by HC could improve neurologic outcome and reduce BBB permeability, apparently through reduced gene transcription-protein synthesis and inflammation.
Huntingtin's Neuroprotective Activity Occurs via Inhibition of Procaspase-9 Processing*

Huntington's Disease is an inherited neurodegenerative disease that affects the medium spiny neurons in the striatum. The disease is caused by the expansion of a polyglutamine sequence in the N terminus of Huntingtin (Htt), a widely expressed protein. Recently, we have found that Htt is an antiapoptotic protein in striatal cells and acts by preventing caspase-3 activity. Here we report that Htt overexpression in other CNS-derived cells can protect them from more than 20 days exposure to fatal stimuli. In particular, we found that cytochrome c continues to be released from mitochondria into the cytosol of cells that overexpress normal Htt. However, procaspase-9 is not processed, indicating that wild-type Htt (wtHtt) acts downstream of cytochrome c release. These data show that Htt inhibits neuronal cell death by interfering with the activity of the apoptosome complex.
Planting Patterns and Deficit Irrigation Strategies to Improve Wheat Production and Water Use Efficiency under Simulated Rainfall Conditions

The ridge furrow (RF) rainwater harvesting system is an efficient way to enhance rainwater accessibility for crops and increase winter wheat productivity in semi-arid regions. However, the RF system has not been promoted widely in the semi-arid regions, which primarily exist in remote hilly areas. To exploit its efficiency on a large-scale, the RF system needs to be tested at different amounts of simulated precipitation combined with deficit irrigation. Therefore, in during the 2015–16 and 2016–17 winter wheat growing seasons, we examined the effects of two planting patterns: (1) the RF system and (2) traditional flat planting (TF) with three deficit irrigation levels (150, 75, 0 mm) under three simulated rainfall intensity (1: 275, 2: 200, 3: 125 mm), and determined soil water storage profile, evapotranspiration rate, grain filling rate, biomass, grain yield, and net economic return. Over the two study years, the RF treatment with 200 mm simulated rainfall and 150 mm deficit irrigation (RF2150) significantly (P < 0.05) increased soil water storage in the depth of (200 cm); reduced ET at the field scale by 33%; increased total dry matter accumulation per plant; increased the grain-filling rate; and improved biomass (11%) and grain (19%) yields. The RF2150 treatment thus achieved a higher WUE (76%) and RIWP (21%) compared to TF. Grain-filling rates, grain weight of superior and inferior grains, and net economic profit of winter wheat responded positively to simulated rainfall and deficit irrigation under both planting patterns. The 200 mm simulated rainfall amount was more economical than other precipitation amounts, and led to slight increases in soil water storage, total dry matter per plant, and grain yield; there were no significant differences when the simulated rainfall was increased beyond 200 mm. The highest (12,593 Yuan ha−1) net income profit was attained using the RF system at 200 mm rainfall and 150 mm deficit irrigation, which also led to significantly higher grain yield, WUE, and RIWP than all other treatments. Thus, we recommend the RF2150 treatment for higher productivity, income profit, and improve WUE in the dry-land farming system of China.