Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional proteomic view of metabolic regulation in “Aromatoleum aromaticum” strain EbN1

The denitrifying “Aromatoleum aromaticum” strain EbN1 utilizes a wide range of aromatic and nonaromatic compounds under anoxic and oxic conditions. The recently determined genome revealed corresponding degradation pathways and predicted a fine‐tuned regulatory network. In this study, differential proteomics (2‐D DIGE and MS) was used to define degradation pathway‐specific subproteomes and to determine their growth condition dependent regulation. Differential protein profiles were determined for cultures adapted to growth under 22 different substrate and redox conditions. In total, 354 different proteins were identified, 199 of which displayed significantly changed abundances. These regulated proteins mainly represented enzymes of the different degradation pathways, and revealed different degrees of growth condition specific regulation. In case of three substrate conditions (e.g. phenylalanine, anoxic), proteins previously predicted to be involved in their degradation were apparently not involved (e.g. Pdh, phenylacetaldehyde dehydrogenase). Instead, previously not considered proteins were specifically increased in abundance (e.g. EbA5005, predicted aldehyde:ferredoxin oxidoreductase), shedding new light on the respective pathways. Moreover, strong evidence was obtained for thus far unpredicted degradation pathways of three hitherto unknown substrates (e.g. o‐aminobenzoate, anoxic). Comparing all identified regulated and nonregulated proteins provided first insights into regulatory hierarchies of special degradation pathways versus general metabolism in strain EbN1.
Moringa oleifera-rich diet and T-cell calcium signaling in hypertensive rats.

Moringa oleifera is a plant whose fruits, roots and leaves have been advocated for traditional medicinal uses. The physico-chemical analysis shows that, Moringa contains more dietary polyunsaturated fatty acids (PUFA) than saturated fatty acids (SFA). The consumption of an experimental diet enriched with Moringa oleifera extracts lowered blood pressure in spontaneously hypertensive rats (SHR), but not in normotensive Wistar-Kyoto (WKY) rats as compared to rats fed an unsupplemented control diet. Anti-CD3-stimulated T-cell proliferation was diminished in both strains of rats fed the Moringa oleifera. The experimental diet lowered secretion of interleukin-2 in SHR, but not in WKY rats compared with rats fed the control diet. Studies of platelets from patients with primary hypertension and from SHR support the notion that the concentration of intracellular free calcium [Ca(2+)]i is modified in both clinical and experimental hypertension. We observed that the basal, [Ca(2+)]i was lower in T cells of SHR than in those of WKY rats fed the control diet. Feeding the diet with Moringa oleifera extracts to WKY rats did not alter basal [Ca(2+)]i in T cells but increased basal [Ca(2+)]i in SHR. Our study clearly demonstrated that Moringa oleifera exerts antihypertensive effects by inhibiting the secretion of IL-2 and modulates T-cell calcium signaling in hypertensive rats.
Vocal fold phase asymmetries in patients with voice disorders: a study across visualization techniques.

PURPOSE To examine differences in vocal fold vibratory phase asymmetry judged from stroboscopy, high-speed videoendoscopy (HSV), and the HSV-derived playbacks of mucosal wave kymography, digital kymography, and a static medial digital kymography image of persons with hypofunctional and hyperfunctional voice disorders. Differences between the methods of visual judgments and objective measures of left-right phase asymmetry were assessed. The findings were compared with those from a previous study with vocally normal speakers. METHOD Forty-nine persons with voice disorders underwent stroboscopy and HSV. The HSV images were processed, resulting in 4 different spatial or kymographic displays. Two types of phase asymmetries, left-right and anterior-posterior, were visually rated. Objective measures of left-right phase asymmetry were obtained. RESULTS From stroboscopy, the HSV playback, and the HSV-derived playbacks, left-right phase symmetry was judged to be symmetrical in 41%, 32%, and 19% of cases, respectively. This difference in playbacks was not seen for anterior-posterior asymmetry. Correlation between visual judgments and objective measures was mild for stroboscopy and moderate to high for all HSV-based playbacks. CONCLUSIONS The use of kymography appears important for judgments of phase asymmetry. Stroboscopy appears to be sensitive, but possibly not specific, to phase asymmetries. Further development of objective measures is warranted for this feature.
ANALISIS CORPORATE GOVERNANCETERHADAP INTEGRITAS LAPORANKEUANGAN

This study aims to analize the effect of corporate governance that influencing integrity of financial statement at the manufacturer companies in Indonesia. Integrity of financial statement define as how far a financial statement disclosure shows truth and honest informations. Corporate governance mechanism that analized the effect to integrity of financial statement are Institusional ownership, manajerial ownership, audit commettee, independent commissioner, audit quality, profitability, leverage, and firm size. This study used quantitative method to financial statement of manufacturer industry that listed in Bursa Efek Indonesia period 2007-2009. Total samples in this study are 83 companies that selected with purposive sampling method. The examination of hypothesis method using logistic regretion. Result of this study indicate that not all of corporate governance mechanism effect to integrity of financial statement. However, institutional ownership, audit commettee, audit quality, profitability, leverage, and firm size provided has a significant influence to increasing integrity of financial statement.
Analysis of prognostic factors and applicability of Kohne's prognostic groups in patients with metastatic colorectal cancer treated with first-line irinotecan or oxaliplatin-based chemotherapy.

BACKGROUND The objective of this study was to analyze prognostic factors for survival and to assess the applicability of Kohne's classification in patients treated with irinotecan- or oxaliplatin-based first-line chemotherapy. PATIENTS AND METHODS One hundred forty-two consecutive cases from a single center were retrospectively reviewed. Median patient age was 62 years. Sixty percent were men. Eastern Cooperative Oncology Group (ECOG) performance status (PS) was 0/1 in 88%. Primary tumor resection (PTR) was performed in 80.6% of patients who initially had stage IV disease. Chemotherapy consisted of fluoropyrimidines or raltitrexed plus irinotecan (50.5%), oxaliplatin (38.5%), or both (11%). Univariate and multivariate analyses for survival were performed using pretreatment patient characteristics. RESULTS Median follow-up was 33.9 months and median overall survival was 15.9 months. Significantly unfavorable prognostic factors were PTR not being performed, disease involvement of >1 organ, liver metastases, undifferentiated histology, EGOG PS>1, increased serum carcinoembryonic antigen and cancer antigen 19.9 levels, hypoalbuminemia, leucocytosis, and elevated alkaline phosphatase and lactate dehydrogenase (LDH) levels. Only ECOG PS, PTR, increased LDH level, no hypoalbuminemia, and number of organs involved retained prognostic value in the multivariate analysis. The incidence and median survival for Kohne's prognostic groups were as follows: good (54.2%; 20 months), intermediate (26.8%; 15.7 months), and poor (19%; 6.8 months). For patients with stage IV disease at presentation, PTR was associated with a significantly longer survival, mainly in patients with an ECOG PS of 0/1. CONCLUSION Eastern Cooperative Oncology Group PS, PTR, serum albumin, increased LDH levels, and organ involvement were the main prognostic indicators in our series. Kohne's prognostic groups, developed in the era of 5-fluorouracil treatment, also seem to be applicable to patients treated with combination chemotherapy. Primary tumor resection should always be considered, especially in patients with an ECOG PS of 0/1. However, the benefit of PTR and multiple-agent chemotherapy is questionable in patients with an ECOG PS of >1.
Human Resource Management in the Hospitality Industry

A background to the industry's workforce human resource management job design recruitment selection appointment and induction performance appraisal training management development job evaluation administration of wages and salaries incentives fringe benefits labour turnover and termination of employment industrial relations law of employment manpower planning, records and statistics organizing human resources labour costs and productivity managing people customer care managing in an international context. Appendices: a review of personnel management in the private sector of the British hospitality industry the IPM code of professional practice in personnel management the IPM recruitment code technology and employment in hotels and catering.
Blood Groups and Other Inherited Characters in Pernicious Anaemia

A SERIOUS feature of pernicious anaemia is the tendency to develop gastric carcinoma. Ths is apparent also among the relatives of patients with pernicious anaemia (Mosbech, I953), which suggests that hereditary factors may determine the association between these two diseases. Recently, attention has been drawn to an association between the ABO blood groups and diseases of the stomach. In carcinoma of the stomach there is an excess of group A aid a deficiency of group 0 (Aird, Bentall and Roberts, 1953), indicating an inherited element in susceptibility to or protection against the disease. These obscrvatioiis suggested that it might be of interest to study the blood groups of patients with pernicious anaemia. This has been done as part of a wider investigation into hereditary factors in pernicious anaemia. The results are reported here together with those of tests for various other readily recognizable genetic characteristics.
ΔNp63 is regulated by BMP4 signaling and is required for early epidermal development in Xenopus

Background: It has been established in several models that the p63 gene has an important role in the development of the epidermis and its derivatives. In Xenopus, only the ΔNp63 isoform of this gene has been cloned and its role during epidermal development remains unknown. Results: In this work, we showed that ΔNp63 is expressed in the nonneural ectoderm since the gastrula stage and that it is regulated by the bone morphogenetic protein 4 (BMP4) signaling pathway. Our in vivo and in vitro experiments demonstrated that ΔNp63 is required in the earliest inductive steps of epidermal development. The overexpression of ΔNp63 caused an increase in epidermal markers with a suppression of neural induction while the blocking of ΔNp63 led to the opposite results. Finally, we found that ΔNp63 acts as an anti‐apoptotic gene, regulating the transcription of some apoptotic and anti‐apoptotic factors. Conclusion: The results suggest that ΔNp63 is an essential gene in early epidermal specification under the control of BMP4. Developmental Dynamics 241:257–269, 2012. © 2011 Wiley Periodicals, Inc.
[Prevalence of ocular manifestation in 198 patients with rheumatoid arthritis: a retrospective study].

PURPOSE To study the prevalence of eye manifestations in the local rheumatoid arthritis (RA) population, as well as to know if the presence of rheumatoid factor (RF) and antinuclear antibodies (ANA) affect this profile. To study if there is association between eye manifestations and patient's articular functional index, age at diagnosis and illness duration. METHODS We reviewed 198 charts of rheumatoid arthritis patients followed-up from 2003 to 2006. They were studied for: prevalence of ophthalmologic manifestations, autoantibody profile, demographic data and Steinbrock's functional index. RESULTS Secondary Sjögren's syndrome was seen in 12.1% of the patients being more common in women (p=0.049) and in patients with greater articular damage (p=0.016). Ulcerative keratitis and scleritis appeared in 2% of patients each. Antinuclear antibodies (ANA) and rheumatoid factor (RF) as well as illness duration and age at diagnosis had no influence in the appearance of eye manifestations. CONCLUSION Secondary Sjögren syndrome was the most common ocular manifestation in patients with RA, mainly in women and patients with greater joint damage.
Option markets : implied information, equity process and risk management

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Effect of excess degradable intake protein on early embryonic development, ovarian steroids, and blood urea nitrogen on days 2, 3, 4, and 5 of the estrous cycle in mature ewes.

Two trials were conducted to determine whether feeding excess degradable intake protein (DIP) during a synchronized estrous cycle and the first 5 d after breeding alters early embryonic development, ovarian steroids, or BUN concentrations in ewes. Ewes were group-fed in Trial 1 (T1) and individually fed in Trial 2 (T2) either 100 (control; T1, n = 15; T2, n = 12) or 200% (high-protein; T1, n = 16; T2, n = 12) of the NRC protein recommendation for maintenance during a synchronized estrous cycle until surgery in the next cycle. Ampullae (AMP), isthmi (IST), and uterine horns (UT) of high-protein and control ewes were removed on d 2 (T1), 3 (T2), 4 (T1), or 5 (T2) after breeding. In T1, jugular blood samples were taken once daily starting on d 2 of the synchronized cycle, and in T2 on d 2, 9, 15, 16, and 17, and in both trials from estrus (d 0) to the day of surgery. Ampullae, IST, and UT flushings were examined microscopically for the presence of embryos, embryo condition, and embryo cell number. There was no trial x treatment interaction (P > or = 0.10), so data for both trials were pooled. Concentrations of BUN were higher (P < 0.05) in high-protein-fed ewes than in control ewes during the synchronized cycle and the first 5 d of the next cycle. Progesterone concentrations of the synchronized cycle did not differ (P > 0.10) between treatments. During the first 5 d of the next cycle, estradiol-17beta concentrations were lower (P = 0.06) in high-protein-fed than in control ewes. Progesterone increased (P < 0.05) to higher concentrations by d 5 in high-protein-fed ewes than in control ewes. More (P < 0.05) embryos were found in AMP of high-protein-fed ewes than in AMP of control ewes on d 4. Fewer (P = 0.05) embryos were found in UT of high-protein-fed ewes than in UT of control ewes on d 4. More embryos were found in UT of high-protein-fed ewes than in UT of control ewes on d 5. Fewer (P = 0.05) embryos were found in IST of high-protein ewes than in the IST of control ewes on d 5. Embryos of high-protein-fed ewes had more (P < 0.05) cells than embryos from control fed ewes on d 5. Feeding ewes excess DIP protein during an estrous cycle and the first 5 d after breeding initially impeded embryo transport; thereafter, embryo transport and development through the oviduct was accelerated.
Germline genetic contributions to risk for esophageal adenocarcinoma, Barrett's esophagus, and gastroesophageal reflux.

BACKGROUND Esophageal adenocarcinoma (EA) is an increasingly common cancer with poor survival. Barrett's esophagus (BE) is the main precursor to EA, and every year 0.12% to 0.5% of BE patients progress to EA. BE typically arises on a background of chronic gastroesophageal reflux (GERD), one of the risk factors for EA. METHODS We used genome-wide association data to investigate the genetic architecture underlying GERD, BE, and EA. We applied a method to estimate the variance explained (array heritability, h(2)g) and the genetic correlation (rg) between GERD, BE, and EA by considering all single nucleotide polymorphisms (SNPs) simultaneously. We also estimated the polygenic overlap between GERD, BE, and EA using a prediction approach. All tests were two-sided, except in the case of variance-explained estimation where one-sided tests were used. RESULTS We estimated a statistically significant genetic variance explained for BE (h(2)g = 35%; standard error [SE] = 6%; one-sided P = 1 × 10(-9)) and for EA (h(2)g = 25 %; SE = 5%; one-sided P = 2 × 10(-7)). The genetic correlation between BE and EA was found to be high (rg = 1.0; SE = 0.37). We also estimated a statistically significant polygenic overlap between BE and EA (one-sided P = 1 × 10(-6)), which suggests, together with the high genetic correlation, that shared genes underlie the development of BE and EA. Conversely, no statistically significant results were obtained for GERD. CONCLUSIONS We have demonstrated that risk to BE and EA is influenced by many germline genetic variants of small effect and that shared polygenic effects contribute to risk of these two diseases.
Optimizing Identification of Clinically Relevant Gram-Positive Organisms by Use of the Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System

ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) can be used as a method for the rapid identification of microorganisms. This study evaluated the Bruker Biotyper (MALDI-TOF MS) system for the identification of clinically relevant Gram-positive organisms. We tested 239 aerobic Gram-positive organisms isolated from clinical specimens. We evaluated 4 direct-smear methods, including “heavy” (H) and “light” (L) smears, with and without a 1-μl direct formic acid (FA) overlay. The quality measure assigned to a MALDI-TOF MS identification is a numerical value or “score. ” We found that a heavy smear with a formic acid overlay (H+FA) produced optimal MALDI-TOF MS identification scores and the highest percentage of correctly identified organisms. Using a score of ≥2.0, we identified 183 of the 239 isolates (76.6%) to the genus level, and of the 181 isolates resolved to the species level, 141 isolates (77.9%) were correctly identified. To maximize the number of correct identifications while minimizing misidentifications, the data were analyzed using a score of ≥1.7 for genus- and species-level identification. Using this score, 220 of the 239 isolates (92.1%) were identified to the genus level, and of the 181 isolates resolved to the species level, 167 isolates (92.2%) could be assigned an accurate species identification. We also evaluated a subset of isolates for preanalytic factors that might influence MALDI-TOF MS identification. Frequent subcultures increased the number of unidentified isolates. Incubation temperatures and subcultures of the media did not alter the rate of identification. These data define the ideal bacterial preparation, identification score, and medium conditions for optimal identification of Gram-positive bacteria by use of MALDI-TOF MS.
Encouraging Mexican-Heritage Youth to Intervene When Friends Drink

Drawing from primary socialization theory, we hypothesized that as Mexican-heritage youth engage in targeted parent-child communication against alcohol, they are more likely to disapprove of and consider the negative consequences of drinking alcohol. In turn, such antialcohol perceptions are likely to encourage them to intervene if a friend was to drink alcohol. The analyses were based on self-reported longitudinal data from 1,149 Mexican-heritage youth in sixth to eighth grades (M = 12 years, SD = .61). As males and females engaged in targeted parent-child communication against alcohol, they were more likely to consider the negative consequences of alcohol consumption. Consequently, they reported that they would be more likely to intervene by talking to the friend or an adult. Disapproving of alcohol consumption played a minor role for male and female Mexican-heritage youth.
Molecular characterization of Pulikulam cattle using microsatellite markers

The present study estimates genetic variability in Pulikulam cattle (n=50), a draught breed of India, by use of 18 microsatellite markers recommended by the Food and Agriculture Organization. Microsatellite genotypes were derived and allelic and genotypic frequencies, heterozygosities and gene diversity were estimated. A total of 142 alleles were distinguished by the 18 microsatellite markers used. All the microsatellites were highly polymorphic, with mean (± s.e.) allelic number of 7.89 ± 0.72, ranging 3-12 per locus. The observed heterozygosity in the population ranged between 0.1316 and 0.8958, with mean (± s.e.) of 0.5758 ± 0.0535, indicating considerable genetic variation in this population. Genetic bottleneck hypotheses were also explored. Our data suggest that the Pulikulam breed has not experienced a genetic bottleneck in the recent past.
Low Temperature and Magnetic Field Performance of Spliced Commercial YBCO CC

The design of next-generation magnets based on 2G high-temperature superconductors requires a careful investigation of the possibilities and performance of joints between different superconducting wire pieces. In this paper, we present a study of the electrical performance of splices made with commercial Superpower ReBCO tapes at both 77 K and 4.2 K. For this study we developed a special splicing device based on the hot-bar soldering technique that allowed estimating the degree of reproducibility of the splicing method in view of future standard series production. During our study we investigated the influence of the splicing parameters (temperature, pressure, duration, flux fluid, solder composition, cooling etc.) on the final joint performance in terms of specific resistance, critical current degradation and n-value degradation. The best performing splice was characterized at 4.2 K in perpendicular magnetic field up to 11 T. The results show an excellent degree of reproducibility with no appreciable degradation of the original tape performances. The ultimate splice resistance seems determined by the intrinsic architecture of the tape rather than by the splicing method itself.
Possible dysregulation of chaperon and metabolic proteins in cystic fibrosis bronchial tissue

Cystic fibrosis (CF) is an autosomal recessive disease due to mutations of the CF transmembrane conductance regulator gene. A systematic approach to generate a protein expressional pattern in CF bronchial tissue has not been performed so far. It was the aim of this hypothesis‐generating study to construct differential proteomes of bronchial biopsies in controls (n = 8) and CF patients (n = 9). Biopsies (pools of three per patient) were taken; proteins were extracted and run on 2‐DE with subsequent in‐gel digestion and mass spectrometrical identification and quantification of proteins using specific software. Three hundred sixty‐six protein spots were identified and compared between groups. Following an approach for multiple testing correction, the chaperone 75 kDa glucose‐regulated protein and ubiquinol–cytochrome c reductase complex core protein I and one form of nidogen, a pseudogene of aconitase 2, were increased in CF (p < 0.005). Aberrant protein levels may reflect molecular changes of CF as well as CF‐linked inflammation, infection and cellular stress response.