Miracle-dz/UniFAR-FTUs huggingface dataset:
EFFECTS OF CROSS AND SELF‐FERTILIZATION ON PROGENY FITNESS IN LOBELIA CARDINALIS AND L. SIPHILITICA

Inbreeding depression, or the decreased fitness of progeny derived from self‐fertilization as compared to outcrossing, is thought to be the most general factor affecting the evolution of self‐fertilization in plants. Nevertheless, data on inbreeding depression in fitness characters are almost nonexistent for perennials observed in their natural environments. In this study I measured inbreeding depression in both survival and fertility in two sympatric, short‐lived, perennial herbs: hummingbird‐pollinated Lobelia cardinalis (two populations) and bumblebee‐pollinated L. siphilitica (one population). Crosses were performed by hand in the field, and seedlings germinated in the greenhouse. Levels of inbreeding depression were determined for one year in the greenhouse and for two to three years for seedlings transplanted back to the natural environment. Fertility was measured as flower number, which is highly correlated with seed production under natural conditions in these populations. Inbreeding depression was assessed in three ways: 1) survival and fertility within the different age intervals; 2) cumulative survival from the seed stage through each age interval; and 3) net fertility, or the expected fertility of a seed at different ages. Net fertility is a comprehensive measure of fitness combining survival and flower number. In all three populations, selfing had nonsignificant effects on the number and size of seeds. Lobelia siphilitica and one population of L. cardinalis exhibited significant levels of inbreeding depression between seed maturation and germination, excluding the consideration of possible differences in dormancy or longterm viability in the soil. There was no inbreeding depression in subsequent survival in the greenhouse in any population. In the field, significant survival differences between selfed and outcrossed progeny occurred only in two years and in only one population of L. cardinalis. For both survival and fertility there was little evidence for the expected differences among families in inbreeding depression. Compared to survival, inbreeding depression in fertility (flower number) tended to be much higher. By first‐year flower production, the combined effects on survival and flower number caused inbreeding depression in net fertility to reach 54%, 34% and 71% for L. siphilitica and the two populations of L. cardinalis. By the end of the second year of flowering in the field, inbreeding depression in net fertility was 53% for L. siphilitica and 54% for one population of L. cardinalis. For the other population of L. cardinalis, these values were 76% through the second year of flowering and 83% through the third year. Such high levels of inbreeding depression should strongly influence selection on those characters affecting self‐fertilization rates in these two species.
Lesbians on Medical Encounters: Tales of Heteronormativity, Deception, and Expectations

The experiences of lesbian women in medical encounters prove particularly relevant for understanding their difficulties in their relationship with professionals and health services. We carried out semistructured interviews with 30 women aged 21 to 63 years, who define themselves as lesbian. The analysis highlights the difficulties experienced in disclosure of sexuality in medical encounters, the tendency for doctors to come across as heteronormative, and also medical practices experienced as appropriate by interviewees. Analysis of participant experiences demonstrates the need for reflection and decision making to promote the recognition of the sexual citizenship of lesbian women and their empowerment.
Simultaneous impairment of passive avoidance learning and nociception in rats following chronic swim stress

Background: Stress can alter response to nociception. Under certain circumstances stress enhances nociception, a phenomenon which is called stress-induced hyperalgesia (SIH). While nociception has been studied in this paradigm, possible alterations occurring in passive avoidance (PA) learning after exposing rats to this type of stress has not been studied before. Materials and Methods: In the current study, we evaluated the effect of chronic swim stress (FS) or sham swim (SS) on nociception in both spinal (tail-flick) and supraspinal (53.5°C hot-pate) levels. Furthermore, PA task was performed to see whether chronic swim stress changes PA learning or not. Mobility of rats and anxiety-like behavior were assessed using open-field test (OFT). Results: Supraspinal pain response was altered by swim stress (hot-plate test). PA learning was impaired by swim stress, rats in SS group did not show such impairments. Rats in the FS group showed increased mobility (rearing, velocity, total distant moved (TDM) and decreased anxiety-like behavior (time spent in center and grooming) compared to SS rats. Conclusions: This study demonstrated the simultaneous impairment of PA and nociception under chronic swim stress, whether this is simply a co-occurrence or not is of special interest. This finding may implicate a possible role for limbic structures, though this hypothesis should be studied by experimental lesions in different areas of rat brain to assess their possible role in the pathophysiology of SIH.
Social Participation of Adolescents with Cerebral Palsy: Trade-offs and Choices

ABSTRACT This article reports on the findings of a qualitative study about the experiences and perceptions of adolescents with cerebral palsy (CP) in relation to social participation. A phenomenological approach was used to interview 10 adolescents with CP, 17 to 20 years of age, selected using purposeful sampling. An iterative process of data collection and analysis resulted in four themes about social participation. The themes of experience, barriers, and supports, and tradeoffs supported the current view of participation as a dynamic interaction between person and environment. The fourth theme of making choices described the unique challenges facing adolescents with CP in terms of deciding what was most important and meaningful to them now and in their future. Health care professionals can support adolescents as they develop the capacity to make their own decisions during the transition to adult living by ensuring that assessments and interventions address social participation.
Role of the Fibula in Weight‐bearing

To investigate the weight-bearing function of the fibula, weight-loading experiments were performed on autopsy specimens. With the ankle joint in neutral position, the weight distribution to the fibula amounted to 6.4%. With dorsiflexion of the ankle joint, the weight on the fibula increased. With plantar flexion of the ankle joint, the weight on the fibula decreased. Lateral and posterior loading of the tibia produced increased weight on the fibula. With eversion of the ankle joint, the weight on the fibula increased. The weight-bearing function increased with augmentation of the tibiofibular articular angle. These characteristics of the fibular weight-bearing function should be considered carefully in the treatment of the knee joint or the fibula.
Physiological Adaptations to Resistance Training in Prepubertal Boys

Purpose: The purpose of this study was to investigate the physiological adaptations of resistance training (RT) in prepubertal boys. Methods: Eighteen healthy boys were divided into RT (n = 9, Mage = 10.4 ± 0.5 years) and control (CTR; n = 9, Mage = 10.9 ± 0.7 years) groups. The RT group underwent a resistance training during 12 weeks, 3 times per week, performing 3 sets of 6 to 15 repetitions at intensities ranging from 60% to 80% of maximal dynamic strength (1-repetition maximum [1-RM] values). Before and after the training, the groups were assessed in their body mass and composition (dual-energy X-ray absorptiometry), isokinetic dynamometry, 1-RM, and ergoespirometry. Moreover, force per unit of muscle volume was calculated by the quotient between 1-RM and lean mass. Results: Both groups presented statistically significant (p < .05) increases in the 1-RM and force per unit of muscle volume in the knee extension and elbow flexion, but these strength increases were statistically significantly greater in the RT group (effect size [ES] = 2.83–9.00) than in the CTR group (ES = 0.72–1.00). Moreover, both groups statistically significantly increased in lean body mass variables (ES = 0.12–0.38). However, increases in the fat mass variables occurred only in the CTR group (ES = − 0.01–0.50), whereas no changes were observed in the RT group. Furthermore, there were statistically significant increases in all bone mineral content variables (ES = 0.13–0.43), without differences between groups. No cardiorespiratory changes were observed. Conclusion: Twelve weeks of RT was effective in improving strength and force per unit of muscle volume and prevented fat mass increases in boys.
Institutional Determinants of Higher Education Students' International Mobility within the Erasmus Programme Countries

One of the major EU policy objectives is to enhance the international mobility of students. The Leuven Communique published in 2009 set an objective of increasing the ratio of European Higher Education Area (EHEA) higher education graduates participating in a study or a training period abroad to at least 20% by 2020. However, currently the majority of European Union Member States perform significantly below the target in this respect. Also, since a low number of students are interested in mobility programmes, the funds of the Erasmus-type student mobility programs remain unused. This study focuses on highlighting the factors that represent barriers to student participation in mobility programs. After conducting a literature review on international student mobility and presenting major statistics describing outbound mobility, this study investigates factors related to institutional components of the higher education system that affect the international mobility of Erasmus young people. Among the explanatory factors related to Erasmus-type student mobility, cultural factors including Hofstede’s indulgence and uncertainty avoidance seem to have the greatest influence on student mobility intentions in Europe. The findings revealed that better planned Erasmus processes (pre-, during and postmobility activities such as departure, course choice, staying in a host country, etc.) and better communicated career opportunities and labour market values of the mobility could considerably contribute to an increase in the number of outbound students. One of the main lessons learned from the conducted analyses is that Europe’s rich cultural diversity needs to be considered in the course of promoting the Erasmus Programme in Europe. In addition to adopting common communication, promotion and direction strategies, programmes need to be elaborated that take national specificities into account.
Nontubercular Mycobacterial Pulmonary Infection in Immunocompetent Men

Background. Nontubercular mycobacteria (NTM) are increasingly recognized to cause lung disease in immunocompetent patients. We studied the occurrence of pulmonary infection due to NTM in immunocompetent men. Methods. We retrospectively analyzed all sputum mycobacterial cultures at our institution over a 5-year period. Charts were reviewed to identify patients who met the American Thoracic Society's criteria for mycobacterial pulmonary infection. Results. From the 7,380 sputum mycobacterial cultures obtained, 46 male patients had NTM identified. Forty-two patients were immunocompetent. Five of these patients were found to have NTM'2 with Mycobacterium kansasii, 2 with Mycobacterium avium-intracellulare, and 1 with Mycobacterium gordonae. All 5 patients responded to antimycobacterial therapy. Conclusion. Twelve percent of our population of immunocompetent men from whom NTM were isolated from sputum were infected. This study should alert the clinician that NTM cause treatable pulmonary disease in immunocompetent men.
Headache characteristics in patients after migrainous stroke

Six patients who fulfilled strictly defined criteria for migrainous cerebral infarction and in whom other causes of stroke were ruled out were observed. All had a long-standing history of migraine with aura. In most, stroke was mild with good recovery and no recurrence. Headache frequency and severity decreased after the stroke. It is hypothesized that the improvement in migraine may be due to reduced nociceptive transmission as result of loss in vasoreactivity of the affected cerebral blood vessel.
Hierarchical gene expression profiles of HUVEC stimulated by different lipid A structures obtained from Porphyromonas gingivalis and Escherichia coli

The ability of lipid A structural variants to elicit unique endothelial cell gene expression was examined by measuring global gene expression profiles in human umbilical cord vein endothelial cells (HUVEC) using Affymetrix full genome chips. Two lipid A structural variants obtained from Porphyromonas gingivalis designated PgLPS1435/1449 and PgLPS1690 as well as LPS obtained from Escherichia coli wild type and an E. coli msbB mutant (missing myristic acid in the lipid A) were examined. Each of these lipid A structures has been shown to interact with TLR4; however, PgLPS1435/1449 and E. coli msbB LPS have been shown to be TLR4 antagonists while PgLPS1690 and wild‐type E. coli LPS are TLR4 agonists. It was found that PgLPS1435/1449 and PgLPS1690 as well as E. coli msbB LPS activated a subset of those genes significantly transcribed in response to E. coli wild‐type LPS. Furthermore, the subset of genes expressed in response to the different lipid A structural forms were those most significantly activated by wild‐type E. coli LPS demonstrating a hierarchy in TLR4‐dependent endothelial cell gene activation. A unique gene expression profile for the weak TLR4 agonist PgLPS1690 was observed and represents a TLR4 hierarchy in endothelial cell gene activation.
New Fungus-Insect Symbiosis: Culturing, Molecular, and Histological Methods Determine Saprophytic Polyporales Mutualists of Ambrosiodmus Ambrosia Beetles

Ambrosia symbiosis is an obligate, farming-like mutualism between wood-boring beetles and fungi. It evolved at least 11 times and includes many notorious invasive pests. All ambrosia beetles studied to date cultivate ascomycotan fungi: early colonizers of recently killed trees with poor wood digestion. Beetles in the widespread genus Ambrosiodmus, however, colonize decayed wood. We characterized the mycosymbionts of three Ambrosiodmus species using quantitative culturing, high-throughput metabarcoding, and histology. We determined the fungi to be within the Polyporales, closely related to Flavodon flavus. Culture-independent sequencing of Ambrosiodmus minor mycangia revealed a single operational taxonomic unit identical to the sequences from the cultured Flavodon. Histological sectioning confirmed that Ambrosiodmus possessed preoral mycangia containing dimitic hyphae similar to cultured F. cf. flavus. The Ambrosiodmus-Flavodon symbiosis is unique in several aspects: it is the first reported association between an ambrosia beetle and a basidiomycotan fungus; the mycosymbiont grows as hyphae in the mycangia, not as budding pseudo-mycelium; and the mycosymbiont is a white-rot saprophyte rather than an early colonizer: a previously undocumented wood borer niche. Few fungi are capable of turning rotten wood into complete animal nutrition. Several thousand beetle-fungus symbioses remain unstudied and promise unknown and unexpected mycological diversity and enzymatic innovations.
Lenalidomide as initial therapy of elderly patients with chronic lymphocytic leukemia.

The best initial therapy for elderly patients with chronic lymphocytic leukemia (CLL) has not yet been defined. We investigated the activity of lenalidomide as initial therapy for elderly patients with CLL. Sixty patients with CLL 65 years of age and older received treatment with lenalidomide orally 5 mg daily for 56 days, then titrated up to 25 mg/d as tolerated. Treatment was continued until disease progression. At a median follow-up of 29 months, 53 patients (88%) are alive and 32 patients (53%) remain on therapy. Estimated 2-year progression-free survival is 60%. The overall response rate to lenalidomide therapy is 65%, including 10% complete response, 5% complete response with residual cytopenia, 7% nodular partial response, and 43% partial response. Neutropenia is the most common grade 3 or 4 treatment-related toxicity observed in 34% of treatment cycles. Major infections or neutropenic fever occurred in 13% of patients. Compared with baseline levels, we noted an increase in serum immunoglobulin levels across all classes, and a reduction in CCL3 and CCL4 plasma levels was noted in responding patients. Lenalidomide therapy was well tolerated and induced durable remissions in this population of elderly, symptomatic patients with CLL. This study was registered at www.clinicaltrials. gov as #NCT00535873.
Repeated invasive lung function measurements in intubated mice: an approach for longitudinal lung research

Invasive lung function measurements are useful tools to describe respiratory disease models in mice but only result in one time-point measurements because of tracheostomy. We explored if intubation may overcome the need for tracheostomy thereby allowing invasive lung function monitoring of individual mice over time. Repeated invasive lung function measurements with Scireq© – FlexiVent or Buxco© – Forced Pulmonary Maneuvers® were performed three times in BALB/c mice with intervals of 10 days. Each lung function assessment following intubation was compared with a similar measurement in age-matched tracheostomized mice, the golden standard in lung function measurements. Tracheostomy and intubation gave similar results for resistance, elastance and compliance of the whole respiratory system as assessed by Flexivent. Likewise, Forced Pulmonary Maneuvers used to measure lung volumes such as total lung capacity, functional residual capacity, forced expiratory volume in 0.1 s and forced vital capacity, resulted in identical outcomes for both airway approaches. No interaction was found between the procedures for any of the pulmonary function variables. The observed changes over time were rather related to animal growth than to repetitive intubation. Eighty percent of the animals survived three consecutive intubations, which were hampered by transient breathing difficulties, weight loss and neutrophilic bronchoalveolar lavage immediately postextubation. Repetitive invasive lung function measurements by intubation are feasible and reproducible in healthy mice and results are comparable to the standard method. This may open new perspectives for longitudinal research in animal models of respiratory diseases.
No Evidence for a Genetic Relationship between Alzheimer’s Disease and Longevity

Objective: Late onset Alzheimer’s disease (AD) is frequent in subjects who have reached an age above the average life expectancy. AD and life expectancy are both influenced by genetic factors. Consequently, a possible genetic relationship between AD and longevity was investigated using family study data. Methods: First-degree relatives of patients with AD (n = 645) and of non-demented controls (n = 1,106) were examined by direct interview or by family history in the case of already deceased or unavailable subjects. Survival of subjects with an assumed familial load for AD (i.e. first-degree relatives of AD patients) and of controls was compared using the Kaplan-Meier analysis and log-rank statistics. Results: Relatives with AD reached higher ages than other family members. However, there was no significant co-aggregation of AD and longevity in first-degree relatives of AD patients in comparison with those of controls. This applied to all diseased as well as non-demented relatives. Conclusions: Longevity is an independent prerequisite for the development of the disease, but is not genetically related to AD. The apparent longevity in relatives with AD is likely to result from the selection of subjects fulfilling this prerequisite.
Provisionnement des rentes viagères en Algérie entre approche statique et approche prospective

Life expectancy at birth in Algeria keeps improving significantly at least since the independence in 1962. This longevity combined with the new constraints of the Algerian life annuity market reduces the usefulness of static life tables and accentuates the need to use prospective life tables as pricing and reserving tools. In the absence of mortality data specific to the annuity market, we propose, in this work, to rely directly on the data of the global population in order to build a prospective life table for life annuities pricing and reserving in Algeria. The results obtained under the prospective approach are compared to those obtained under the static approach.
Cobalt ions influence proliferation and function of human osteoblast-like cells

Cobalt is the major component in many orthopedic implants and the introduction of a second generation of metal on metal bearing prosthesis systems actualizes the toxicity and biocompatibility of this compound. We studied the effect of cobalt ions on primary cultures of human osteoblast-like cells. Cobalt ions dissolved in cell culture medium caused a dose-dependent decrease in proliferation of human osteoblasts measured as ( 3 H)thymidine incorporation. We also found that cobalt ion-enriched medium increased the production of interleukin-6 from the osteoblast-like cells. Furthermore, incubation of osteoblasts with cobalt ion-enriched medium reduced collagen type I and osteocalcin production in a dose-dependent manner when 1,25 dihydroxyvitamin-D 3 was added to the culture medium. Cobalt concentrations below 10 µg/mL or 0.17 mmol/mL in the cell culture medium had no significant effect on human osteoblast proliferation and function.
Recent observed changes in severe storms over the United Kingdom and Iceland

Severe storms defined as 3‐hourly pressure changes exceeding an extreme magnitude, were carefully manually quality‐controlled and analyzed at stations in the UK and Iceland which had at least 45 years of digitized data. Iceland showed significant distribution differences between the periods before and after 1980 with a tendency towards less extreme severe events in latter decades. In contrast, the UK regions have tended towards larger magnitude events in recent decades, particularly in the more southerly regions. There has been a significant increase in the number of severe storms over the UK as a whole since the 1950s, however, this may not be unusual in longer‐term variability. For both the UK and Iceland in winter these changes in severe storms appear to be related to changes in the North Atlantic Oscillation (NAO) but UK changes during October to December do not appear to be related to changes in the NAO.