Miracle-dz/UniFAR-FTUs huggingface dataset:
Anatomical coupling among distributed cortical regions in youth varies as a function of individual differences in vocabulary abilities

Patient lesion and functional magnetic resonance imaging (fMRI) studies have provided convincing evidence that a distributed brain network subserves word knowledge. However, little is known about the structural correlates of this network within the context of typical development and whether anatomical coupling in linguistically relevant regions of cortex varies as a function of vocabulary skill. Here we investigate the association between vocabulary and anatomical coupling in 235 typically developing youth (ages 6–19 years) using structural MRI. The study's primary aim was to evaluate whether higher vocabulary performance was associated with greater vertex‐level cortical thickness covariation in distributed regions of cortex known to be associated with word knowledge. Results indicate that better vocabulary skills are associated with greater anatomical coupling in several linguistically relevant regions of cortex, including the left inferior parietal (temporal‐parietal junction), inferior temporal, middle frontal, and superior frontal gyri and the right inferior frontal and precentral gyri. Furthermore, in high vocabulary scorers, stronger coupling is found among these regions. Thus, complementing patient and fMRI studies, this is the first investigation to highlight the relevance of anatomical covariance within the cortex to vocabulary skills in typically developing youth, further elucidating the distributed nature of neural systems subserving word knowledge. Hum Brain Mapp 35:1885–1895, 2014. © 2013 Wiley Periodicals, Inc.
Migration of individual microvessel endothelial cells: stochastic model and parameter measurement.

Analysis of cell motility effects in physiological processes can be facilitated by a mathematical model capable of simulating individual cell movement paths. A quantitative description of motility of individual cells would be useful, for example, in the study of the formation of new blood vessel networks in angiogenesis by microvessel endothelial cell (MEC) migration. In this paper we propose a stochastic mathematical model for the random motility and chemotaxis of single cells, and evaluate migration paths of MEC in terms of this model. In our model, cell velocity under random motility conditions is described as a persistent random walk using the Ornstein-Uhlenbeck (O-U) process. Two parameters quantify this process: the magnitude of random movement accelerations, alpha, and a decay rate constant for movement velocity, beta. Two other quantities often used in measurements of individual cell random motility properties--cell speed, S, and persistence time in velocity, Pv--can be defined in terms of the fundamental stochastic parameters alpha and beta by: S =square root (alpha/beta) and Pv = 1/beta. We account for chemotactic cell movement in chemoattractant gradients by adding a directional bias term to the O-U process. The magnitude of the directional bias is characterized by the chemotactic responsiveness, kappa. A critical advantage of the proposed model is that it can generate, using experimentally measured values of alpha, beta and kappa, computer simulations of theoretical individual cell paths for use in evaluating the role of cell migration in specific physiological processes. We have used the model to assess MEC migration in the presence of absence of the angiogenic stimulus acidic fibroblast growth factor (aFGF). Time-lapse video was used to observe and track the paths of cells moving in various media, and the mean square displacement was measured from these paths. To test the validity of the model, we compared the mean square displacement measurements of each cell with model predictions of that displacement. The comparison indicates that the O-U process provides a satisfactory description of the random migration at this level of comparison. Using nonlinear regression in these comparisons, we measured the magnitude of random accelerations, alpha, and the velocity decay rate constant, beta, for each cell path. We consequently obtained values for the derived quantities, speed and persistence time. In control medium, we find that alpha = 250 +/- 100 microns 2h-3 and beta = 0.22 +/- 0.03h-1, while in stimulus medium (control plus unpurified aFGF) alpha = 1900 +/- 720 microns 2h-3 and beta = 0.99 +/- 0.37h-1. (ABSTRACT TRUNCATED AT 400 WORDS)
What Are the Most Important Symptom Targets When Treating Advanced Cancer? A Survey of Providers in the National Comprehensive Cancer Network (NCCN)

We derived a set of brief, clinically relevant symptom indices for assessing symptomatic response to chemotherapy for advanced bladder, brain, breast, colorectal, head and neck, hepatobiliary/pancreas, lung, ovarian, and prostate cancers. Questions were extracted from a multidimensional cancer quality of life (QOL) measurement system, the Functional Assessment of Cancer Therapy (FACT). Surveys of disease-related symptoms were presented to expert physicians and nurses at 17 National Comprehensive Cancer Network (NCCN) member institutions. In a two-step procedure, each expert narrowed the list to no more than five of the very most important to attend to when assessing the value of drug treatment for advanced disease. Symptoms endorsed at a frequency greater than chance probability were retained for the nine symptom indices. The resulting NCCN/FACT symptom indices are comprised of 6–15 items, depending on disease. Fatigue, pain, nausea, weight loss, worry about worsening condition, and contentment with current QOL were consistently selected by experts as priority symptoms across tumor sites. These nine tumor-specific symptom indices indicate the most important clinician-rated targets of chemotherapy for many advanced cancers. These results await validation in patient populations and examination of the extent to which changes in symptomatology translate into meaningful improvement to the patient.
Intragenomic complementation of a 3AB mutant in dicistronic polioviruses.

We report the construction of a poliovirus genome [pPVM-VPg(3F4A)] harboring a double mutation in VPg. This mutant, in which the tyrosine and the threonine at residues 3 and 4 of the VPg region were replaced by phenylalanine and alanine, respectively, is lethal, that is, all RNA synthesis was abolished and no revertants could be isolated. Using the properties of dicistronic polioviruses (with the general genotype PV 5'NTR-3AB-EMCV IRES-PV ORF-3'NTR), we have observed that the defect in RNA synthesis of the VPg(3F4A) mutant could be rescued by providing wild-type protein 3AB from the first open reading frame in trans. We conclude that the 3AB provided by the first cistron of the dicistronic construct was capable of "intragenomic complementation. " Intragenomic complementation, however, was inefficient. Thus, the dicistronic RNAs were only quasi-infectious, and even first-passage viruses were found to have reverted to a functioning VPg in the polyprotein. This phenomenon underlines the role of polypeptide 3AB in multiple functions of viral proliferation. First-passage viruses, all of which expressed a small-plaque phenotype, had retained the original dicistronic genotype. At the fourth passage, however, all isolates were monocistronic, and they displayed complex genetic rearrangements revealing interesting information regarding IRES function.
Deformation und Zerfall von Flugzeugwirbelschleppen in turbulenter und stabile geschichteter Atmosphäre

Flugzeugwirbelschleppen leben neueren Beobachtungen zufolge deutlich langer als zumeist angenommen. Nachfolgende Flugzeuge, die in die Wirbelschleppe gelangen, erfahren ein Rollmoment, welches von der Deformation der Wirbel abhangt. Daher wird in dieser Arbeit mit Grobstruktursimulationen (LES) die Dynamik von Wirbelschleppen in der turbulenten und geschichteten Atmosphare untersucht. Insbesondere wurde ein Verfahren entwickelt, welches den Verlauf der Wirbelkernlinie prazise identifiziert und daraus erstmalig die Krummung dieser Linie abschnittsweise ermittelt. Die Ergebnisse zeigen, dass sich die mittleren Krummungsradien von Einzelwirbeln vor der Ringbildung bis zu einer Grosenordnung des ursprunglichen Wirbelabstands reduzieren, und danach wieder groser werden konnen. Das zu erwartende Rollmoment eines nachfolgenden Flugzeugs wird abgeschatzt. Insbesondere Vergleiche mit Feldmessungen aus dem EU-Projekt AWIATOR bestatigen die hier gefundenen Ergebnisse.
Phenolic Composition and Evaluation of Methanol and Aqueous Extracts of Bitter Gourd (Momordica charantia L) Leaves on Angiotensin-I-Converting Enzyme and Some Pro-oxidant-Induced Lipid Peroxidation In Vitro

This study sought to assess the effects of bitter gourd leaf extracts (methanol and aqueous) on enzyme linked with hypertension (angiotensin-I-converting enzyme and some pro-oxidants (iron sulfate, sodium nitroprusside, and cisplatin]–induced lipid peroxidation in rat kidney homogenates. Chromatographic analysis of the extract was done using high-performance liquid chromatography–diode array detection. The results revealed that methanol extract (IC50 = 109.63 µg/mL) had significantly (P < .05) higher angiotensin-I converting enzyme inhibitory activity than aqueous extract (IC50 = 182.95 µg/mL). Similarly, methanol extract had significantly (P < .05) higher inhibitory effect on Fe2+- and cisplatin-induced lipid peroxidation than aqueous extract. However, there was no significant (P > .05) difference in the inhibition of sodium nitroprusside–induced lipid peroxidation of both extracts. High-performance liquid chromatography–diode array detection analysis revealed the presence of quercetin, caffeic, gallic, and chlorogenic acids in the leaf. Thus, inhibition of angiotensin-I converting enzyme activity and the antioxidant properties of the extracts could be linked to the presence of phenolic phytochemicals.
Distributed Beamforming for Spectrum-Sharing Systems With AF Cooperative Two-Way Relaying

We consider in this paper distributed beamforming for two-way cognitive radio networks in an effort to improve the spectrum efficiency and enhance the performance of the cognitive (secondary) system. In particular, we consider a spectrum sharing system where a set of amplify-and-forward (AF) relays are employed to help a pair of secondary transceivers in the presence of multiple licensed (primary) users. The set of relays participate in the beamforming process, where the optimal beamformer weights are obtained via a linear optimization method. For this system, we investigate the transmission protocols over two and three time-slots. To study and compare the performance tradeoffs between the two transmission protocols, for both of them, we derive closed-form expressions for the cumulative distribution function (CDF) and the moment generating function (MGF) of the equivalent end-to-end signal-to-noise ratio (SNR) at the secondary receiver. We analyze the performance of the proposed methods where closed-form expressions for the user outage probability and the average error probability are derived for independent and identically distributed Rayleigh fading channels. Numerical results demonstrate the efficacy of beamforming in enhancing the secondary system performance in addition to mitigating the interference to the primary users. In addition, our results show that the three time-slot protocol outperforms the two time-slot protocol in certain scenarios where it offers a good compromise between bandwidth efficiency and system performance.
Effect of Organic Complex Compounds on Bacillus thermoamylovorans Growth and Glucose Fermentation

ABSTRACT The effect of the concentration of a mixture (1/1 [wt/wt]) of yeast extract and bioTrypcase (YE+bT) on the growth and physiology of a new species, Bacillus thermoamylovorans, a moderately thermophilic, non-spore-forming, lactic acid-producing bacterium isolated from palm wine, was studied. At an initial glucose concentration of 100 mM, B. thermoamylovorans growth was limited when the concentration of YE+bT was lower than 5.0 g liter−1; under these conditions, cellular yield reached a maximum value of 0.4 g of cells per g of YE+bT. Growth limitation due to deficiency in growth factors led to a significant shift in glucose metabolism towards lactate production. Lactate constituted 27.5 and 76% of the end products of glucose fermentation in media containing YE+bT at 20.0 and 1.0 g liter−1, respectively. This result markedly differed from published data for lactic bacteria, which indicated that fermentative metabolism remained homolactic regardless of the concentration of YE. Our results showed that the ratio between cellular synthesis and energy production increased with the concentration of YE+bT in the culture medium. They indicate that the industrial production of lactic acid through glucose fermentation by B. thermoamylovorans can be optimized by using a medium where glucose is present in excess and the organic additives are limiting.
Multiple genetic pathways regulate replicative senescence in telomerase‐deficient yeast

Most human tissues express low levels of telomerase and undergo telomere shortening and eventual senescence; the resulting limitation on tissue renewal can lead to a wide range of age‐dependent pathophysiologies. Increasing evidence indicates that the decline in cell division capacity in cells that lack telomerase can be influenced by numerous genetic factors. Here, we use telomerase‐defective strains of budding yeast to probe whether replicative senescence can be attenuated or accelerated by defects in factors previously implicated in handling of DNA termini. We show that the MRX (Mre11‐Rad50‐Xrs2) complex, as well as negative (Rif2) and positive (Tel1) regulators of this complex, comprise a single pathway that promotes replicative senescence, in a manner that recapitulates how these proteins modulate resection of DNA ends. In contrast, the Rad51 recombinase, which acts downstream of the MRX complex in double‐strand break (DSB) repair, regulates replicative senescence through a separate pathway operating in opposition to the MRX‐Tel1‐Rif2 pathway. Moreover, defects in several additional proteins implicated in DSB repair (Rif1 and Sae2) confer only transient effects during early or late stages of replicative senescence, respectively, further suggesting that a simple analogy between DSBs and eroding telomeres is incomplete. These results indicate that the replicative capacity of telomerase‐defective yeast is controlled by a network comprised of multiple pathways. It is likely that telomere shortening in telomerase‐depleted human cells is similarly under a complex pattern of genetic control; mechanistic understanding of this process should provide crucial information regarding how human tissues age in response to telomere erosion.
Structural determinants for membrane trafficking and G protein selectivity of a mouse olfactory receptor

The G protein‐coupled olfactory receptor (OR) superfamily plays a critical role in recognizing a broad range of odorants. Each OR appears to recognize odorants based on similarities in molecular structures such that mOR‐EG, a mouse OR, binds eugenol, vanillin, and some other structurally related odorants. Only a few ORs, however, have been characterized functionally due to the difficulties in expressing ORs in heterologous cells. In this report, we demonstrate roles of the N‐ and C‐terminal domains as key elements in the functional expression and signal transducing activity of an OR. Disruption of the N‐terminal glycosylation site of the mOR‐EG completely impaired its membrane trafficking to the cell surface. Functional expression of the mOR‐EG was greatly enhanced by addition of extra N‐terminal glycosylation sequences. Addition of a C‐terminal epitope‐tag or C‐terminal truncation significantly reduced the odorant‐response activity, although the receptors were properly targeted to the plasma membrane. Analysis of a series of truncated ORs revealed a region in the C‐terminus that was crucial for the receptor activity. Replacement of the C‐terminal portion of the mOR‐EG with that of rhodopsin disrupted the coupling to Gαs but not to Gα15, demonstrating that the C‐terminus is involved in regulating G protein specificity. These results suggest that glycosylation of the N‐terminal portion is critical for OR expression and membrane trafficking, while the C‐terminal portion plays a role in defining proper conformation, which, in turn, specifies the G protein selectivity of the OR. This information helps clarify the mechanisms that regulate membrane trafficking and G protein interaction of the OR superfamily.
Hierarchical Decentralized Optimization Architecture for Economic Dispatch: A New Approach for Large-Scale Power System

In this paper, a new hierarchical decentralized optimization architecture is proposed to solve the economic dispatch problem for a large-scale power system. Conventionally, such a problem is solved in a centralized way, which is usually inflexible and costly in computation. In contrast to centralized algorithms, in this paper we decompose the centralized problem into local problems. Each local generator only solves its own problem iteratively, based on its own cost function and generation constraint. An extra coordinator agent is employed to coordinate all the local generator agents. Besides, it also takes responsibility to handle the global demand supply constraint based on a newly proposed concept named virtual agent. In this way, different from existing distributed algorithms, the global demand supply constraint and local generation constraints are handled separately, which would greatly reduce the computational complexity. In addition, as only local individual estimate is exchanged between the local agent and the coordinator agent, the communication burden is reduced and the information privacy is also protected. It is theoretically shown that under proposed hierarchical decentralized optimization architecture, each local generator agent can obtain the optimal solution in a decentralized fashion. Several case studies implemented on the IEEE 30-bus and the IEEE 118-bus are discussed and tested to validate the proposed method.
Rakeness-Based Design of Low-Complexity Compressed Sensing

Compressed Sensing (CS) can be introduced in the processing chain of a sensor node as a mean to globally reduce its operating cost, while maximizing the quality of the acquired signal. We exploit CS as a simple early-digital compression stage that performs a multiplication of the signal by a matrix. The operating costs (e.g., the consumed power) of such an encoding stage depend on the number of rows of the matrix, but also on the value and position of the rows’ coefficients. Our novel design flow yields optimized sparse matrices with very few rows. It is a non-trivial extension of the rakeness-based approach to CS and yields an extremely lightweight stage implemented by a very small number of possibly signed sums with an excellent compression performance. By means of a general signal model we explore different corners of the design space and show that, for example, our method is capable of compressing the signal by a factor larger than 2.5 while not considering 30% of the original samples (so that they may not be acquired at all, leaving the analog front-end and ADC stages inactive) and by processing each of the considered samples with not more than three signed sums.
High‐affinity binding of melatonin by human circulating T lymphocytes (CD4+)

This paper shows the presence of high‐ affinity binding sites for melatonin in human circu‐lating T lymphocytes, but not in B lymphocytes. The binding of melatonin to T cells was dependent on time, stable, reversible, saturable, specific, and in‐versely correlated to the production of melatonin, expressed as the nocturnal 12 h production of its urinary metabolite 6‐sulfatoxymelatonin. The affin‐ity of these binding sites ( K d = 0.27 nM) suggests that they may recognize the physiological concentrations of melatonin in serum. Moreover, among the lymphocyte subpopulations studied, binding of melatonin was mostly found in CD4+ cells rather than in CD8+ cells. Results suggest that CD4+ cells may be the target of melatonin among the human circulating lymphocytes. —Gonzalez‐Haba, M. G. , Garcia‐Mauriño, S. , Calvo, J. R. , Goberna, R. , Guerrero, J. M. High‐affinity binding of melatonin by human circulating T lymphocytes (CD4+). FASEB J. 9, 1331‐1335(1995)
Genomic DNA fingerprinting, using pulsed-field gel electrophoresis, of Staphylococcus intermedius isolated from dogs.

OBJECTIVES To investigate the degree of polymorphism in the pulsed-field gel electrophoresis (PFGE) patterns of Staphylococcus intermedius and to assess the value of this typing method for discriminating strains. SAMPLE POPULATION 52 S intermedius isolates from diseased and healthy dogs. PROCEDURE Chromosomal DNA of S intermedius was digested with restriction endonuclease Sma I, and the fragments were separated by PFGE in a 1% agarose gel. RESULTS Sma I cut the chromosomal DNA into 15 to 23 fragments ranging from about < 1 to 679 kb, and most of the detectable fragments were < 155 kb. Nine fragments, 115, 48, 33, 26, 16, 13, 10, 4, and < 1 kb, were shared by all or almost all (> 71%) of the strains examined. Of the 52 strains, each had a different pattern. S intermedius had a high degree of restriction fragment length polymorphism. The PFGE patterns obtained for S intermedius were stable and reproducible when the strains were tested in the different experiments. CONCLUSIONS Genomic DNA fingerprinting by PFGE is an effective technique for discriminating S intermedius strains. The PFGE method appears to be a useful molecular marker for epidemiologic or ecologic studies of S intermedius.
Maternal nutrient restriction affects properties of skeletal muscle in offspring

Maternal nutrient restriction (NR) affects fetal development with long‐term consequences on postnatal health of offspring, including predisposition to obesity and diabetes. Most studies have been conducted in fetuses in late gestation, and little information is available on the persistent impact of NR from early to mid‐gestation on properties of offspring skeletal muscle, which was the aim of this study. Pregnant ewes were subjected to 50% NR from day 28–78 of gestation and allowed to deliver. The longissimus dorsi muscle was sampled from 8‐month‐old offspring. Maternal NR during early to mid‐gestation decreased the number of myofibres in the offspring and increased the ratio of myosin IIb to other isoforms by 17.6 ± 4.9% (P < 0.05) compared with offspring of ad libitum fed ewes. Activity of carnitine palmitoyltransferase‐1, a key enzyme controlling fatty acid oxidation, was reduced by 24.7 ± 4.5% (P < 0.05) in skeletal muscle of offspring of NR ewes and would contribute to increased fat accumulation observed in offspring of NR ewes. Intramuscular triglyceride content (IMTG) was increased in skeletal muscle of NR lambs, a finding which may be linked to predisposition to diabetes in offspring of NR mothers, since enhanced IMTG predisposes to insulin resistance in skeletal muscle. Proteomic analysis by two‐dimensional gel electrophoresis demonstrated downregulation of several catabolic enzymes in 8‐month‐old offspring of NR ewes. These data demonstrate that the early to mid‐gestation period is important for skeletal muscle development. Impaired muscle development during this stage of gestation affects the number and composition of fibres in offspring which may lead to long‐term physiological consequences, including predisposition to obesity and diabetes.