Miracle-dz/UniFAR-FTUs huggingface dataset:
The expression of caspase-3, caspase-7, caspase-9 and cytokeratin AE1/AE3 in goats with enzootic nasal adenocarcinoma: an immunohistochemical study.

The aim of this study was to examine the expression of caspase-3, caspase-7, caspase-9 and cytokeratin AE-1/AE-3 using the avidin-biotin complex (ABC) immunoperoxidase technique in 20 goats with enzootic nasal adenocarcinoma (ENA). Clinically, dyspnoea and nasal discharge were observed in all cases. Macroscopically, polypoid and sessile masses were seen in the ethmoidal area. At the histopathological examination, tubular, papil - lary and mixed patterns of ENA were diagnosed. Immunohistochemically, strong positive reactions were generally seen for caspase-3, while strong to moderate and slight reactions were observed for caspase-7 and caspase-9 in the cytoplasm of the tumour cells. Positive reactions for cytokeratin AE-1/AE-3 were only seen in epithelial cells. In addition, the causative agent of ENA, retrovirus, was detected immunohistochemically in tumour cells.
Evidence for a Hexaheteromeric Methylenetetrahydrofolate Reductase in Moorella thermoacetica

ABSTRACT Moorella thermoacetica can grow with H2 and CO2, forming acetic acid from 2 CO2 via the Wood-Ljungdahl pathway. All enzymes involved in this pathway have been characterized to date, except for methylenetetrahydrofolate reductase (MetF). We report here that the M. thermoacetica gene that putatively encodes this enzyme, metF, is part of a transcription unit also containing the genes hdrCBA, mvhD, and metV. MetF copurified with the other five proteins encoded in the unit in a hexaheteromeric complex with an apparent molecular mass in the 320-kDa range. The 40-fold-enriched preparation contained per mg protein 3.1 nmol flavin adenine dinucleotide (FAD), 3.4 nmol flavin mononucleotide (FMN), and 110 nmol iron, almost as predicted from the primary structure of the six subunits. It catalyzed the reduction of methylenetetrahydrofolate with reduced benzyl viologen but not with NAD(P)H in either the absence or presence of oxidized ferredoxin. It also catalyzed the reversible reduction of benzyl viologen with NADH (diaphorase activity). Heterologous expression of the metF gene in Escherichia coli revealed that the subunit MetF contains one FMN rather than FAD. MetF exhibited 70-fold-higher methylenetetrahydrofolate reductase activity with benzyl viologen when produced together with MetV, which in part shows sequence similarity to MetF. Heterologously produced HdrA contained 2 FADs and had NAD-specific diaphorase activity. Our results suggested that the physiological electron donor for methylenetetrahydrofolate reduction in M. thermoacetica is NADH and that the exergonic reduction of methylenetetrahydrofolate with NADH is coupled via flavin-based electron bifurcation with the endergonic reduction of an electron acceptor, whose identity remains unknown.
Rearrangement of MYC is associated with poor prognosis in patients with diffuse large B-cell lymphoma treated in the era of rituximab.

PURPOSE Rearrangement of MYC occurs in a proportion of diffuse large B-cell lymphomas (DLBCL), where they may be associated with an adverse clinical outcome. The aim of this study was to determine the frequency of MYC translocations in DLBCL and their prognostic impact in the era of cyclophosphamide, doxorubicin, vincristine, and prednisone plus rituximab (CHOP-R) therapy. PATIENTS AND METHODS Three hundred three patients with previously untreated DLBCL, with no evidence of underlying follicular lymphoma, were investigated using immunohistochemistry and interphase fluorescent in situ hybridization for MYC, BCL6, and t(14;18)/BCL2 rearrangements. All patients (median age, 71.1 years; range, 23 to 96 years) were treated when CHOP-R was standard therapy for DLBCL and observed for a maximum of 4 years. Overall survival (OS) at 3 years was 49% (95% CI, 42% to 56%). RESULTS MYC rearrangements were demonstrated in 35 (14%) of 245 biopsies with data available. Of these, 26 (74%) also had a t(14;18), 10 (26%) were BCL6 and MYC rearranged, and seven had all three abnormalities. Only age, International Prognostic Index, and MYC rearrangement retained prognostic significance in the final model. OS was significantly worse for patients with rearrangement of MYC (survival probability at 2 years = 0.35 in v 0.61 in the nonrearranged group). CONCLUSION The presence of a MYC rearrangement is a strongly adverse prognostic factor in CHOP-R-treated patients and can be used in combination with patients' age and IPI to accurately predict clinical outcome. In DLBCL, rearrangement of MYC is rarely found as the sole genetic abnormality and the poor prognosis of these patients is likely to reflect a synergistic effect alongside deregulation of BCL6 or BCL2.
Impact of Magnetic Labeling on Human and Mouse Stem Cells and Their Long-Term Magnetic Resonance Tracking in a Rat Model of Parkinson Disease

Magnetic resonance imaging (MRI) of magnetically labeled stem cells has become a valuable tool in the understanding and evaluation of experimental stem cell–based therapies of degenerative central nervous system disorders. This comprehensive study assesses the impact of magnetic labeling of both human and rodent stem cell–containing populations on multiple biologic parameters as maintenance of stemness and oxidative stress levels. Cells were efficiently magnetically labeled with very small superparamagnetic iron oxide particles. Only under the condition of tailored labeling strategies can the impact of magnetic labeling on vitality, proliferation, pluripotency, and oxidative stress levels be minimized. In a rat model of Parkinson disease, magnetically labeled mouse embryonic stem cells were tracked by high-field MRI for 6 months. Significant interindividual differences concerning the spatial distribution of cells became evident. Histologically, transplanted green fluorescent protein–positive iron oxide–labeled cells were clearly identified. No significant increase in oxidative stress levels at the implantation site and no secondary uptake of magnetic label by host phagocytotic cells were observed. Our study strongly suggests that molecular MRI approaches must be carefully tailored to the respective cell population to exert minimal physiologic impact, ensuring the feasibility of this imaging approach for clinical applications.
Precise Candidate Selection for Large Character Set Recognition by Confidence Evaluation

This paper proposes a precise candidate selection method for large character set recognition by confidence evaluation of distance-based classifiers. The proposed method is applicable to a wide variety of distance metrics and experiments on Euclidean distance and city block distance have achieved promising results. By confidence evaluation, the distribution of distances is analyzed to derive the probabilities of classes in two steps: output probability evaluation and input probability inference. Using the input probabilities as confidences, several selection rules have been tested and the rule that selects the classes with high confidence ratio to the first rank class produced best results. The experiments were implemented on the ETL9B database and the results show that the proposed method selects about one-fourth as many candidates with accuracy preserved compared to the conventional method that selects a fixed number of candidates.
Proliferation and progesterone receptor status in benign meningiomas are not age dependent

Some authors have suggested that the biology of meningiomas differs according to a patient's age. Proliferation, vascularity, and hormonal status in meningiomas can be used to describe changes during aging. In the current study, proliferation activity with the Ki‐67/MIB‐1 antibody was evaluated by immunohistochemistry in meningioma tissue specimens from young and elderly patients.
Expression of neurotransmitter receptors and Ca2+ channels in the adult fornix and optic nerve

Using Xenopus oocytes, we have investigated the presence of mRNA coding for neurotransmitter receptors and channels in the adult bovine fornix and optic nerve. Oocytes injected with mRNA from either area, expressed comparable levels of functional receptors to several neuro- transmitters, including glutamate and angiotensin II, as well as voltage-activated Ca2+ channels. Glutamate receptors were mainly of the AMPA/kainate type and were expressed in astrocyte cultures from the regions studied, suggesting that the receptors are present at least in this type of glial cells in vivo. These results show that the white matter areas in the adult brain express receptors and channels. These molecules may play an important role in the transfer of information between axons and glial cells.
CHAOS-2—a geomagnetic field model derived from one decade of continuous satellite data

SUMMARY We have derived a model of the near-Earth's magnetic field using more than 10 yr of high-precision geomagnetic measurements from the three satellites Orsted, CHAMP and SAC-C. This model is an update of the two previous models, CHAOS (Olsen et al. 2006) and xCHAOS (Olsen & Mandea 2008). Data selection and model parameterization follow closely those chosen for deriving these models. The main difference concerns the maximum spherical harmonic degree of the static field (n= 60 compared to n= 50 for CHAOS and xCHAOS), and of the core field time changes, for which spherical harmonic expansion coefficients up to n= 20 are described by order 5 splines (with 6-month knot spacing) spanning the years from 1997.0 to 2009.5. Compared to its predecessors, the temporal regularization of the CHAOS-2 model is also modified. Indeed, second and higher order time derivatives of the core field are damped by minimizing the second time derivative of the squared magnetic field intensity at the core–mantle boundary. The CHAOS-2 model describes rapid time changes, as monitored by the ground magnetic observatories, much better than its predecessors.
Myelographic findings in spondylosis of the lumbar spine.

Lumbar spondylosis can produce nerve root or cauda equina compression. The hypertrophic facet joints, laminal processes and osteophytes of spondylosis may produce defects both at the anterior, posterior and lateral margins of the Pantopaque column in myelography. Multiple constrictions of the oil column in upright lumbar myelography are characteristic features of spondylosis. Differentiating myelographic features of spondylosis from herniation of intervertebral disc are discussed, and 11 illustrated cases are presented. In lumbar spondylosis, the most frequently involved areas, in order of their sequence, are L4–5, L3–4 and L2–3; whereas in herniation of the nucleus pulposus they are L4–5 and L5–S1.
Apoptosis: a potential triggering mechanism of neurological manifestation in Plasmodium falciparum malaria

Plasmodium falciparum infection can lead to a life threatening disease and the pathogenetic mechanisms of severe manifestations are not fully understood. Here, we investigated the capacity of P. falciparum‐parasitized red blood cells (PRBC) from 45 children with clinical malaria to induce endothelial cell (EC) apoptosis.
Phase II Trial of Etoposide, Doxorubicin, and Cisplatin Combination in Advanced Measurable Gastric Cancer: An Eastern Cooperative Oncology Group Study

A phase II study was performed to determine the efficacy and toxicity of the etoposide, doxorubicin, cisplatin (EAP) regimen in the treatment of patients with advanced measurable gastric cancer in a multi-institutional cooperative group setting. Thirty-one evaluable patients with advanced measurable gastric adenocarcinoma were treated with etoposide 120 mg/m2 on days 3, 4, and 5, doxorubicin 20 mg/m2 on days 1 and 8, and cisplatin 40 mg/m2 on days 2 and 9. The treatment was repeated every 28 days. Objective responses were seen in 7 (23%) patients, all achieving partial remissions. Median survival was 9 months for the entire group. Toxicity was mostly hematologic, with grade 3 leukopenia in 26% and grade 4 leukopenia in 55% of the patients. There were 4 treatment-related deaths that were attributable to severe leukopenia and sepsis. Because of the high toxicity and moderate response rate, this regimen is not superior to other less toxic regimens and cannot be recommended for the treatment of advanced gastric cancer outside of an investigational protocol.
Gastric mucosa-associated lymphoid tissue in autoimmune thyroid diseases.

BACKGROUND There is increasing evidence for a link between Helicobacter pylori infection and the development of lymphoid follicles in the gastric mucosa. It is not known whether other factors may also play a role. The aim of this study was to investigate the role played by the host with peculiar immunogenic disorders, in the presence or absence of a known antigenic stimulus such as H. pylori. For this, we studied patients with autoimmune thyroid diseases. METHODS Thirty patients with autoimmune thyroid diseases and 30 dyspeptic patients without a history of thyroid disorders (as control group) underwent upper endoscopy. Lymphoid follicles and H. pylori status were assessed by histopathologic and enzymatic analysis. RESULTS Organized mucosa-associated lymphoid tissue was found in 73.3% of the patients and in 33.3% of control group. Lymphoid follicles were found in 87.5% of the H. pylori-positive patients and in 57.1% of the H. pylori-negative patients (P = NS). In the control group these follicles were present in 50% of H. pylori-positive subjects and in 14.3% of those who were H. pylori-negative. CONCLUSIONS lYMPHOID follicles in the gastric mucosa are common in autoimmune thyroid diseases. Besides H. pylori infection, other factors (environmental, unknown infectious agents) or mechanisms related to the underlying disease may play a causal role.
Arithmetic Disabilities, Specific and Otherwise

From a neuropsychological perspective, this article summarizes research that addresses some of the developmental interactions of disabilities in reading, spelling, and mechanical arithmetic. The focus is on two subtypes of children with learning disabilities who exhibit equally impaired levels of arithmetic achievement, but with vastly different patterns of neuropsychological assets and deficits. Qualitative as well as quantitative analyses lead to the conclusion that one of these patterns of neuropsychological assets and deficits (i.e., the nonverbal learning disabilities syndrome; NLD) leads—at the same time and in much the same manner—to specific patterns of impairment in mechanical arithmetic and in psychosocial functioning. The other pattern (Group R-S) is found to lead to particular patterns of academic deficits (including arithmetic), but not to any particular level or type of psychosocial dysfunction. The manifestations of the NLD profile in various types of neurological disease, disorder, and dysfunction are also explored.
The 2.2 A resolution crystal structure of influenza B neuraminidase and its complex with sialic acid.

Influenza virus neuraminidase catalyses the cleavage of terminal sialic acid, the viral receptor, from carbohydrate chains on glycoproteins and glycolipids. We present the crystal structure of the enzymatically active head of influenza B virus neuraminidase from the strain B/Beijing/1/87. The native structure has been refined to a crystallographic R‐factor of 14.8% at 2.2 A resolution and its complex with sialic acid refined at 2.8 A resolution. The overall fold of the molecule is very similar to the already known structure of neuraminidase from influenza A virus, with which there is amino acid sequence homology of approximately 30%. Two calcium binding sites have been identified. One of them, previously undescribed, is located between the active site and a large surface antigenic loop. The calcium ion is octahedrally co‐ordinated by five oxygen atoms from the protein and one water molecule. Sequence comparisons suggest that this calcium site should occur in all influenza A and B virus neuraminidases. Soaking of sialic acid into the crystals has enabled the mode of binding of the reaction product in the putative active site pocket to be revealed. All the large side groups of the sialic acid are equatorial and are specifically recognized by nine fully conserved active site residues. These in turn are stabilized by a second shell of 10 highly conserved residues principally by an extensive network of hydrogen bonds.
Aspectos do Manejo Nutricional de Bubalinos para Abate: Uma Revisão

Esta revisao foi realizada com o objetivo de identificar aspectos especificos sobre exigencias nutricionais de bubalinos para abate. Foram revisados aspectos anatomo-fisiologicos relacionados a nutricao de bubalinos, fatores envolvidos no controle do consumo de alimentos, e informacoes e tabelas de exigencias de proteina, energia, macroelementos minerais calcio, fosforo, magnesio, sodio e potassio, e de vitaminas.
Category Fluency in a Latino Sample: Associations with Age, Education, Gender, and Language

The authors know of no published studies that have evaluated the effect of Spanish- versus English-language on category fluency within a sample of United States Latinos only. As part of a pilot study for the institution of a cognitive screening program in a cohort of Latinos, we assessed category fluency (fruits, vegetables, and “other” supermarket items) in a sample of 90 self-identified Latino community residents (aged 52-84, 0-18 years of education). The primary demographic correlates of category fluency were age and education. The decrement in naming of fruits with age was limited to the older old subjects (>age 70). Relatively younger old subjects (aged 61-70) did not differ from middle-aged subjects on category fluency. Gender showed little relationship to category naming. Persons naming in Spanish named significantly fewer ‘other supermarket’ items, but did not differ from English speakers in the more common fluency categories of fruits and vegetables. This analysis of category fluency in an ethnically homogenous sample with a wide range of formal education provided an evaluation of the effects of chosen language free of possible confounding by cultural differences, and also provided a more complete evaluation of the influence of education on category fluency.
Special Issue: Knowledge, Knowing, and Organizations: Islands of Shared Knowledge: Specialization and Mutual Understanding in Problem-Solving Teams

Where should the boundaries of knowledge between economic actors be located in order to maximize the efficiency of their interactions? In particular, what circumstances determine whether it makes sense for adjacent stages in the value chain to invest in the development of common understanding, and when does it make sense for them to operate in mutual ignorance? To address these questions, it is necessary to construct a kind of production function which takes different forms of knowledge -- specialist capability and trans-specialist understanding --as inputs and relates them to problem-solving output.Using a simple model of product design as a template, it is possible to derive such a production function. One can then use its properties in conjunction with plausible assumptions about the cost of acquiring different kinds of knowledge to develop general principles that explain when efficiency requires costly learning across specialties and when it is better to allow specialties to operate in mutual ignorance of one another's domains. The derived production function, contrary to some intuition and previous literature, implies that specialist capability cansubstitute for knowledge shared across specialties. This result in turn implies that the nature of learning costs, rather than the shape of knowledge benefits, plays the predominant role in determining when mutual ignorance is a good idea.The analysis also helps resolve the following paradox: The economy depends for its efficiency upon a drastic separation of knowledge across individuals and organizational units, yet studies of product development find that greater knowledge commonality is associated with better firm performance. An implication of the substitutability of specialized and trans-specialty knowledge is that situations where learning across specialties is desirable seem relatively rare in the economy as a whole. These situations are disproportionately common, however, in those areas where important managerial activity takes place. A key role of management is to attend to the strategic, operational, and governance needs of these "islands of shared knowledge in a sea of mutual ignorance. "
Antidepressant up-titration: pharmacological and psychological considerations

The use of antidepressant up-titration in starting therapies is useful in reducing side effects, but can induce delays in clinical response. Several aspects concerning slow or more aggressive titration have to be considered when using this strategy, such as pharmacological aspects (e.g., synaptic up-regulation) and psychological/clinical aspects (e.g., adherence to antidepressant treatment, and the temperament and expectations of patients). Data from the literature are controversial, and so the choice between rapid and slow titration must be tailored more to the patient, rather than to the drug.
Cobweb posets as noncommutative prefabs

A class of new type graded infinite posets with minimal element are considered. These so called cobweb posets introduced recently by the present author provide a wide range of new noncommutative prefab combinatorial schema with characteristic graded subposets as primes. The schema are defined here via relaxing commutativity and associativity requirements imposed on the composition of prefabs by the fathers of this fertile concept. The construction and the very first basic properties of cobweb prefabs are pointed out in what follows. An another single valued commutative amd associative composision is also considered.
Cost-effectiveness models for dental caries prevention programmes among Chilean schoolchildren.

AIM This study aims to estimate the cost-effectiveness from a societal perspective of seven dental caries prevention programmes among schoolchildren in Chile: three community-based programmes: water-fluoridation, salt-fluoridation and dental sealants; and four school-based programmes: milk-fluoridation; fluoridated mouthrinses (FMR); APF-Gel, and supervised toothbrushing with fluoride toothpaste. METHODS Standard cost-effectiveness analysis methods were used. The costs associated with implementing and operating each programme, using a societal perspective, were identified and estimated. The comparator was non-intervention. Health outcomes were measured as dental caries averted over a 6-year period. Costs were estimated as direct treatment costs, programmes costs and costs of productivity losses as a result of each dental caries prevention programme. Incremental cost-effectiveness ratios were calculated for each programme. Sensitivity analyses were conducted over key parameters. RESULTS Primary cost-effectiveness analysis (discounted) indicated that four programmes showed net social savings by the DMFT averted. These savings encompassed a range of values per diseased tooth averted; US$16.21 (salt-fluoridation), US$14.89 (community water fluoridation); US$14.78 (milk fluoridation); and US$8.63 (FMR). Individual programmes using an APF-Gel application, dental sealants, and supervised tooth brushing using fluoridated toothpaste, represent costs for the society per diseased tooth averted of US$21.30, US$11.56 and US$8.55, respectively. CONCLUSION Based on cost required to prevent one carious tooth among schoolchildren, salt fluoridation was the most cost-effective, with APF-Gel ranking as least cost-effective. Findings confirm that most community/school-based dental caries interventions are cost-effective uses of society's financial resources. The models used are conservative and likely to underestimate the real benefits of each intervention.