Miracle-dz/UniFAR-FTUs huggingface dataset:
Sequence and analysis of zona pellucida 2 cDNA (ZP2) from a marsupial, the brushtail possum, Trichosurus vulpecula

All mammalian eggs are surrounded by the zona pellucida, an extracellular coat involved in vital functions during fertilization and early development. The zona pellucida glycoproteins are promising antigenic targets for development of contraceptive vaccines to control pest populations of marsupials in Australia and New Zealand. Our current understanding of the function of the zona pellucida glycoproteins is based almost entirely on the mouse and may not be representative of gamete interactions in all eutherian or marsupial mammals. This study reports the isolation and characterization of the ZP2 gene from the brushtail possum (Trichosurus vulpecula). The brushtail possum ZP2 mRNA is 2,182 nucleotides long with an open reading frame coding for a polypeptide chain of 712 amino acids with a molecular mass of 79,542 d. The deduced amino acid sequence of possum ZP2 is 48 to 55% identical to that of eutherian mammals. It shares several structural characteristics including N‐linked glycosylation sites, location and number of cysteine residues, and hydropathy profile. The brushtail possum ZP2gene is expressed exclusively in the ovary. Further studies are planned to elucidate the specific site of ZP2 expression within the ovary and its function during fertilization in marsupials. Mol. Reprod. Dev. 51:322–329, 1998. © 1998 Wiley‐Liss, Inc.
Performance of bundle protocol for deep-space communications

Delay/disruption tolerant networking (DTN) was developed to enable automated network communications despite frequent and lengthy link disruptions and long link delay. DTN communications rely heavily on a bundle protocol (BP) that uses the well-known approach of store and forward with optional custody transfer for which a node agrees to hold BP data units, bundles, in memory (or storage) until its successful reception is acknowledged by the next node. However, in the presence of long link disruptions, little work has been done regarding performance modeling of BP for reliable data transfer in deep-space communications. In this paper, we present a study of BP over a relay-based deep-space communications system, characterized by lengthy link disruptions accompanied by an extremely long propagation delay, lossy data links, and asymmetric channel rates. An analytical model is built to estimate the total delivery time of a file (message) that is transmitted as the contents of one or more BP bundles over a deep-space channel. A model is also built to characterize the dynamics of memory occupancy and release when BP is used for reliable data delivery in the presence of link disruptions. The constructed models are validated by running experiments using a test bed.
Relationship Between Chewing Rate and Masticatory Performance

Abstract The influence of mandibular movement timing on food breakdown remains unclear. The authors, therefore, sought to relate chewing rate with masticatory performance. Chewing rate, defined as the number of masticatory cycles habitually achieved per minute, was measured in 55 healthy dentulous subjects (age, 22.2±5.0 years). Subjects were grouped according to obtained values (cycles/minute): slower: <70; middle: 70-90; and faster: >90. Masticatory performance was determined through the sieve method, and the estimated comminuted median particle size (X50). Data was analyzed using the one-way ANOVA and chi-square tests (α=.05). Subjects with slower chewing rates showed higher (p<.05) masticatory performance (X50 = 3.05±0.77 mm). X50 was associated with chewing rate when subjects were categorized as better or poorer performers (chi-square=11.25, p<.005). Thus, chewing rate was related to masticatory performance, with smaller food particles being achieved with a slower chewing rate.
Environmental CO2 inhibits Caenorhabditis elegans egg-laying by modulating olfactory neurons and evokes widespread changes in neural activity

Significance Carbon dioxide (CO2) gradients are ubiquitous, but fluctuations in CO2 provide an important cue shaping animal behavior. This paradox suggests that CO2 provides contextual information that is integrated with other inputs. Here, we show that Caenorhabditis elegans CO2-sensing circuits are much more sophisticated than assumed hitherto. A surprisingly large number of neurons, including nociceptors, gustatory neurons, and olfactory neurons, respond to CO2 in vivo. Glia also exhibit large Ca2+ responses to CO2. Worms therefore may couple detection of CO2 and other cues at the earliest stages of sensory processing. Besides avoiding CO2, C. elegans stops laying eggs at high CO2. Inhibition of oviposition involves sustained activation of the AWC olfactory neurons by CO2 and enduring inhibition of neurons innervating the egg-laying muscles. Carbon dioxide (CO2) gradients are ubiquitous and provide animals with information about their environment, such as the potential presence of prey or predators. The nematode Caenorhabditis elegans avoids elevated CO2, and previous work identified three neuron pairs called “BAG,” “AFD,” and “ASE” that respond to CO2 stimuli. Using in vivo Ca2+ imaging and behavioral analysis, we show that C. elegans can detect CO2 independently of these sensory pathways. Many of the C. elegans sensory neurons we examined, including the AWC olfactory neurons, the ASJ and ASK gustatory neurons, and the ASH and ADL nociceptors, respond to a rise in CO2 with a rise in Ca2+. In contrast, glial sheath cells harboring the sensory endings of C. elegans’ major chemosensory neurons exhibit strong and sustained decreases in Ca2+ in response to high CO2. Some of these CO2 responses appear to be cell intrinsic. Worms therefore may couple detection of CO2 to that of other cues at the earliest stages of sensory processing. We show that C. elegans persistently suppresses oviposition at high CO2. Hermaphrodite-specific neurons (HSNs), the executive neurons driving egg-laying, are tonically inhibited when CO2 is elevated. CO2 modulates the egg-laying system partly through the AWC olfactory neurons: High CO2 tonically activates AWC by a cGMP-dependent mechanism, and AWC output inhibits the HSNs. Our work shows that CO2 is a more complex sensory cue for C. elegans than previously thought, both in terms of behavior and neural circuitry.
A moderate excess of dietary lysine lowers plasma and tissue carnitine concentrations in pigs

This study was performed to investigate whether dietary lysine concentration influences the carnitine status of pigs. Therefore, an experiment with twenty young pigs with an average body weight of 21 kg was performed which were fed either a control diet (9·7 g lysine/kg) or a diet with a moderate excess of lysine (16·8 g lysine/kg). Concentrations of all the other amino acids did not differ between the diets. Pigs fed the high-lysine diet had lower concentrations of free and total carnitine in plasma, liver, kidney and skeletal muscle than control pigs (P < 0·05). Pigs fed the high-lysine diet moreover had an increased concentration of trimethyllysine (TML), a reduced mRNA abundance of TML dioxygenase and reduced concentrations of γ-butyrobetaine (BB) in muscle, indicating that the conversion of TML into BB in muscle was impaired. Concentrations of BB, the metabolic precursor of carnitine, in plasma, liver and kidney were also reduced in pigs fed the high-lysine diet while the activity of BB dioxygenase in kidney was not different and that in liver was even increased compared to control pigs (P < 0·05). In conclusion, this study shows that a moderate dietary excess of lysine lowers plasma and tissue carnitine concentrations in pigs. Reduced concentrations of BB in liver and kidney suggest that the depressed carnitine status was likely caused by a decreased rate of carnitine synthesis due to a diminished availability of carnitine precursor, probably mainly as a result of an impaired BB formation in muscle.
Role of Tc-99m Sestamibi Scintigraphy in the Diagnosis and Surgical Decision-Making Process in Primary Hyperparathyroid Disease

Purpose Ultrasound (US) and scintigraphy are used most frequently of all the available imaging techniques for the preoperative evaluation of patients with possible primary hyperparathyroid disease. The aim of this study was to assess the value of dual-phase Tc-99m MIBI scintigraphy compared with US in the detection of adenomatous or hyperplastic glands and in the surgical decision-making process for patients with a biochemical diagnosis of primary hyperparathyroid disease. Methods Ninety-seven patients with increased levels of parathyroid hormone and calcium, and at least 6 months’ follow-up after US and scintigraphy, were examined retrospectively to assess the influence of the diagnostic work-up on the therapeutic decision of the referring clinicians and to evaluate the sensitivity of these diagnostic tools in the surgically treated patients. Forty-eight patients underwent surgery. Results Parathyroid adenomas were found in 43 patients and hyperplasia in 1, whereas 4 patients had no evidence at surgery. The sensitivity and specificity rates were 84.4% and 95.9% for scintigraphy, and 66.6% and 98.6% for US, respectively. Of the 49 nonsurgically treated patients, 35 had negative results with both MIBI and US; only 3 had positive findings with both imaging methods. Patients treated conservatively had significantly lower parathyroid hormone and serum calcium levels than did the patients who had surgery. Conclusions The data suggest that the high sensitivity of dual-phase MIBI scintigraphy can improve the detection of hyperfunctioning parathyroid glands. Furthermore, despite the controversy surrounding the use of imaging methods in the preoperative assessment of primary hyperparathyroid disease, these data suggest that the decision of the clinician to order surgery for a patient with a moderate increase of serum PTH level may be influenced by the results of the imaging methods.
Ralstonia eutropha TF93 Is Blocked in Tat-Mediated Protein Export

ABSTRACT Ralstonia eutropha (formerly Alcaligenes eutrophus) TF93 is pleiotropically affected in the translocation of redox enzymes synthesized with an N-terminal signal peptide bearing a twin arginine (S/T-R-R-X-F-L-K) motif. Immunoblot analyses showed that the catalytic subunits of the membrane-bound [NiFe] hydrogenase (MBH) and the molybdenum cofactor-binding periplasmic nitrate reductase (Nap) are mislocalized to the cytoplasm and to the inner membrane, respectively. Moreover, physiological studies showed that the copper-containing nitrous oxide reductase (NosZ) was also not translocated to the periplasm in strain TF93. The cellular localization of enzymes exported by the general secretion system was unaffected. The translocation-arrested MBH and Nap proteins were enzymatically active, suggesting that twin-arginine signal peptide-dependent redox enzymes may have their cofactors inserted prior to transmembrane export. The periplasmic destination of MBH, Nap, and NosZ was restored by heterologous expression of Azotobacter chroococcum tatAmobilized into TF93. tatA encodes a bacterial Hcf106-like protein, a component of a novel protein transport system that has been characterized in thylakoids and shown to translocate folded proteins across the membrane.
ZR-convolute analysis and modeling: Plasma evolution and dynamics leading to current losses

Post-hole convolutes are used in high-power transmission line systems and join several individual transmission lines in parallel, transferring the combined currents to a single transmission line attached to a load. Magnetic insulation of electron flow, established upstream of the convolute region, is lost at the convolute due, in part, to the formation of magnetic nulls, resulting in current losses. At very high-power operating levels, the formation of electrode plasmas is considered likely which can lead to additional losses. A recent computational analysis of the Sandia Z accelerator suggested that modest plasma desorption rates in the convolute region could explain measured current losses [1]. The recently completed Sandia ZR accelerator has utilized new convolute designs to accommodate changes to the parallel-plate transmission lines on ZR. Detailed particle-in-cell simulations that are fully electromagnetic and relativistic, and include plasma desorption from electrode surfaces in the post-hole convolutes, are carried out to assess the measured current losses on ZR. We find that the plasma desorption rate used to model the Z convolute also applies to three different ZR convolute designs that have been fielded. Based on these findings, the simulation model is being used to develop newer convolute designs with the goal of reducing the current losses, particularly for higher-impedance loads.
Comparative evaluation of midazolam and ketamine with midazolam alone as oral premedication

Background : Oral premedication with midazolam and ketamine is widely used in pediatric anesthesia to reduce emotional trauma and ensure smooth induction. However, various dosing regimens when used alone or in combination have variable efficacy and side effect profile. The aim of our study was to investigate and compare the efficacy of oral midazolam alone with a low‐dose combination of oral midazolam and ketamine.
More aggressive bone marrow screening in retinoblastoma patients is not indicated: The memorial Sloan‐Kettering cancer center experience

Bone marrow involvement in retinoblastoma patients is rare in the more industrialized nations. The purpose of the current study was to determine the frequency of bone marrow involvement in our series of retinoblastoma patients and to investigate whether the use of four bone marrow aspirates (BMA) and two bone marrow biopsies (BMB) has greater sensitivity for the detection of metastatic disease compared to what has been previously reported.
A Thickened Formula Does Not Reduce Apneas Related to Gastroesophageal Reflux in Preterm Infants

Background: Apnea of prematurity (AOP) occurs frequently in preterm infants and a variable proportion of AOP can be induced by gastroesophageal reflux (GER). Conservative treatment, including dietary modifications, should be the first-line approach for both GER and GER-related apneas in this population. Objectives: To evaluate the efficacy of a starch-thickened preterm formula (PF) in reducing the frequency of apneas related to GER. Methods: Preterm infants with AOP were studied by combined impedance and pH monitoring and polysomnography. The 6-hour study period included two feeds, one of a commercially available PF and one of the same formula thickened with amylopectin (TPF). GER indexes, apneas and GER-related apneas detected after TPF and PF feeds were compared by Wilcoxon signed-rank test. Results: 24 infants were studied. During 140 h of registration, 289 apneas (147 after TPF and 142 after PF; p = 0.876), and 861 GER episodes (400 after TPF and 461 after PF; p = 0.465) were recorded. No difference in the number of AOP was found between TPF and PF. A significant reduction in acid exposure was found after TPF; there was no influence on non-acid GER indexes. The frequency of GER-related apneas did not differ between TPF and PF. Conclusions: A formula thickened with amylopectin did not reduce the number of AOP or GER-related apneas. It reduced acid GER features but had no effect on non-acid GER indexes. Future research should focus on exploring different conservative strategies to treat GER-related apneas in preterm infants.
Caenorhabditis elegans fertilization-defective mutants with abnormal sperm.

Seven new fertilization-defective mutants of C. elegans have been isolated and characterized; six are temperature sensitive, one is absolute and all are autosomal recessive. One mutation is in a previously described gene, while the other six define six new fer genes that appear to code for sperm-specific functions necessary for normal fertilization. In all fer mutants, both males and hermaphrodites accumulate sperm in near normal numbers. In hermaphrodites, mutant sperm contact the oocytes, but fail to fertilize them. Instead, the sperm are swept into the uterus by the passing oocytes and are expelled when oocytes are laid. Males of two fer mutants do not transfer sperm during copulation, but the other mutant males transfer sperm that fail to move to the spermatheca. Spermatozoa from fer-1 and fer-4 mutants are motility-defective in vitro as well as in vivo, and their pseudopods have an altered morphology. The period of development during which mutant hermaphrodites are temperature sensitive for fertility overlaps the time of sperm development. Some mutants are temperature sensitive throughout the entire period, and others are temperature sensitive during or just prior to spermiogenesis. In fer-4/+ and fer-7/+ males, the fertility of the mutation-bearing sperm is diminished, reducing the transmission ratio. This implies some post-meiotic expression of these genes. --This set of mutants provides a variety of functional and structural alterations in nematode sperm that should help identify and analyze gene products involved in sperm morphogenesis and motility.
Reluctance of French patients with type 1 diabetes to undergo pig pancreatic islet xenotransplantation

Abstract: Introduction: Type 1 diabetes could possibly be treated by transplantation of pig pancreatic islets. In addition to medical difficulties and ethical problems, social hurdles may need to be overcome. We have evaluated the attitude of patients with type 1 diabetes to the xenotransplantation of pig pancreatic islets and to the potential risks associated with such treatment.
Severe anaemia in childhood cerebral malaria is associated with profound coma.

BACKGROUND Severe anaemia in children with cerebral malaria has been associated with respiratory distress secondary to lactic acidosis and/or hypoxia. The ensuing metabolic derangement may further depress the level of consciousness culminating in presentation with profound coma. This association has poorly been studied. OBJECTIVE To determine the relationship between profound coma at presentation and the presence of severe anaemia in children with cerebral malaria. METHODS This cross-sectional study involved 100 children with cerebral malaria who were consecutively recruited at admission in the Paediatric emergency unit of Mulago hospital in Uganda from July to December 2000. Clinical and laboratory evaluation was done using the hospital's guidelines for the management of severe malaria. The exposure factor of interest was severe anaemia (Hb < 5.0 g/dl) and occurrence of profound coma (Blantyre coma Scale 0) was the outcome measure. RESULTS Severe anaemia and profound coma were seen in 20% and 9% of the children respectively. Severe anaemia was independently associated with profound coma, adjusted OR 1.34 (CI 1.17 - 1.95), p = 0. 002 and age < 3 years, adjusted OR 1.42 (CI 1.13 - 1.54), p = 0.001). Thirty percent of those with severe anaemia had deep sighing (acidotic) breathing compared to only 15% of those with haemoglobin (Hb) > 5 g/dl, OR 1.21 (CI 0.90 - 1.64), p = 0.118. There was no association between the malaria parasite density and severe anaemia. A similar proportion of those with severe anaemia regained consciousness within 24 hours compared to those with Hb > 5 g/dl (30 vs 42.5%), OR 1.56 (0.65 - 3.71), p = 0.307. CONCLUSIONS The findings suggest that profound coma in cerebral malaria may not only result from primary malaria encephalitis but possibly also from a metabolic dysfunction due to severe anaemia.
Comparison of the Effects of Valsartan and Felodipine on Plasma Leptin and Insulin Sensitivity in Hypertensive Obese Patients

Aim of this study was to compare the effect of valsartan and felodipine on blood pressure (BP), plasma leptin (L), insulin sensitivity and plasma norepinephrine (NE) in obese hypertensive patients. Ninty-six obese patients (body mass index [BMI] ≥30 kg/m2) with mild to moderate essential hypertension (diastolic blood pressure [DBP] >90 and <110 mmHg, as evaluated with an appropriately sized cuff) aged 31–60 years, were randomized to a valsartan (80 mg/day for 16 weeks; n=48) or felodipine (5 mg/day for 16 weeks; n=48) treatment group after a 2-week wash-out period. After the first 4 weeks of treatment there was a titration with dose-doubling in non responder patients (DBP > 90 mmHg). At the end of the placebo period and of active treatment period, BP and BMI were evaluated and a venous sample was drawn at the same hour in the morning to evaluate plasma L and NE. Insulin resistance index (HOMA-IR) was calculated. No dietary advice was prescribed. Both valsartan and felodipine significantly decreased BP values (-19.3/15 mmHg and -18.9/13.6 mmHg, respectively p<0.001 vs. placebo), with no difference between treatments. However, felodipine increased plasma NE (+124 pg/ml, +38.2%, p<0.05 vs. placebo and <0.01 vs. valsartan) and had no effect on L, body weight and HOMA-IR index, while valsartan did not modify NE and produced a significant reduction in L (-3.7 ng/ml, -10.1%, p<0.05 vs. placebo), BMI (-1.7 kg/m2, -4.7%, p<0.01 vs. placebo) and HOMA-IR index (-1.6, -20%, p<0.05 vs. placebo). These results suggest that in hypertensive obese subjects, treatment with valsartan might offer an advantage over treatment with felodipine, since valsartan may help to improve obesity-related disorders in addition to lowering BP.
Os Odontoideum Revisited: The Case for a Multifactorial Etiology

Study Design. Retrospective analysis of our experience with os odontoideum at the Children’s Hospital of Philadelphia. Objective. To review the origin of os odontoideum and provide evidence for two separate etiologies. Summary of Background Data. The etiology of os odontoideum has been debated in the literature. Most authors support a post-traumatic etiology; however, some evidence exists to support a congenital origin. Methods. We reviewed all 519 abnormal cervical spine radiographs performed from 1991 to 2004 to identify os odontoideum. Medical records and imaging studies were examined to determine: history of trauma, severity of injury, interval from injury to presentation, coexisting syndromes, and associated congenital cervical spine anomalies. Results. Sixteen of 519 patients (3.1%) had os odontoideum. Only 8 of 16 patients reported previous trauma. Only 3 of these 8 injuries occurred with an interval remote enough to allow remodeling of the dens to an ossicle by the time of presentation. Six of 16 patients had associated congenital anomalies in the cervical spine. Three of 16 had a coexisting genetic syndrome. Conclusion. Our data supports two separate etiologies for the os odontoideum: post-traumatic and congenital. The data should raise awareness that some children with preexisting syndromes may develop os odontoideum without previous trauma.