Miracle-dz/UniFAR-FTUs huggingface dataset:
C‐MYC and IGF‐II mRNA‐binding protein (CRD‐BP/IMP‐1) in benign and malignant mesenchymal tumors

Mouse coding region determinant–binding (mCRD‐BP) and human IGF‐II mRNA–binding 1 (hIMP‐1) proteins are orthologous mRNA‐binding proteins that recognize c‐myc and IGF‐II mRNA, respectively, and regulate their expression posttranscriptionally. Here, we confirm that human CRD‐BP/IMP‐1 binds to c‐myc mRNA and that it is predominantly expressed in fetal tissues. Moreover, hCRD‐BP/IMP‐1 expression was detected in cell lines of neoplastic origin and in selected primary tumors. In a series of 33 malignant and 10 benign mesenchymal tumors, 73% and 40%, respectively, were found to express hCRD‐BP/IMP‐1. In particular, expression was significant in 14 Ewing's sarcomas, all of which were positive. The data suggest that hCRD‐BP/IMP‐1 plays a role in abnormal cell proliferation in mesenchymal tumors. © 2001 Wiley‐Liss, Inc.
Positive end-expiratory pressure delays the progression of lung injury during ventilator strategies involving high airway pressure and lung overdistention

ObjectiveMany studies have investigated the protective role of positive end-expiratory pressure (PEEP) on ventilator-induced lung injury. Most assessed lung injury in protocols involving different ventilation strategies applied for the same length of time. This study, however, set out to investigate the protective role of PEEP with respect to the time needed to reach similar levels of lung injury. DesignProspective, randomized laboratory animal investigation. SettingThe University Laboratory of Ospedale Maggiore, Milano, IRCCS. SubjectsAnesthetized, paralyzed, and mechanically ventilated Sprague-Dawley rats. InterventionsThree groups of five Sprague-Dawley rats were ventilated using zero end-expiratory pressure ZEEP (PEEP of 0 cm H2O) and PEEP of 3 and 6 cm H2O and a similar index of lung overdistension (Pawp/P100 ≅ 1.1; where Pawp is peak airway pressure and P100 is the pressure corresponding to total lung capacity). To obtain this, tidal volume was reduced depending on the PEEP. To reach similar levels of lung injury, we measured respiratory system elastance while ventilating the animals and killed them when respiratory system elastance was 150% of baseline. Once target respiratory system elastance was reached, the lung wet-to-dry ratio was obtained. ResultsRats were ventilated with comparable high airway pressure (Pawp of 42.8 ± 3.1, 43.5 ± 2.6, and 46.2 ± 4.4, respectively, for PEEP 0, 3, and 6) obtaining similar overdistension (Pawp/P100 − index of overdistension: 1.17 ± 0.2, 1.06 ± 0.1, and 1.19 ± 0.2). The respiratory system elastance target was reached and wet-to-dry ratio was not different in the three groups, suggesting a similar degree of lung damage. The time taken to achieve the target respiratory system elastance was three times longer with PEEP 3 and 6 (55 ± 14 mins and 60 ± 17) as compared with zero end-expiratory pressure (18 ± 3 mins, p < .001). ConclusionThese findings confirm that PEEP is protective against ventilator-induced lung injury and may enable the clinician to “buy time” in the progression of lung injury.
Acute resistance exercise with blood flow restriction effects on heart rate, double product, oxygen saturation and perceived exertion

The aim of this study was to compare the acute effect of resistance exercise (RE) with and without blood flow restriction (BFR) on heart rate (HR), double product (DP), oxygen saturation (SpO2) and rating of perceived exertion (RPE). Twenty‐four men (21·79 ± 3·21 years) performed three experimental protocols in a random order (crossover): (i) high‐intensity RE at 80% of 1RM (HI), (ii) low‐intensity RE at 20% of 1RM (LI) and (iii) low‐intensity RE at 20% of 1RM combined with partial blood flow restriction (LI+BFR). HR, blood pressure, SpO2 and RPE were assessed. The data were analysed using repeated measures analysis of variance and the Wilcoxon test for RPE. The results indicated that all protocols significantly increased HR, both immediately postexercise and during the subsequent 60 min (P<0·05), and postexercise DP (P<0·05), but there were no differences between protocols. The protocols of LI and LI+BFR reduced postexercise SpO2 (P = 0·033, P = 0·007), and the LI+BFR protocol presented a perception of greater exertion in the lower limbs compared with HI (P = 0·022). We conclude that RE performed at low intensity combined with BFR seems to reduce the SpO2 after exercise and increase HR and DP while maintaining a perception of greater exertion on the lower limbs.
Antioxidant and Anti-inflammatory Properties of Algerian Thymelaea microphylla Coss. and Dur. Extracts

Background: Thymelaea microphylla Coss. et Dur. (Thymelaeaceae) (TM) is a rare medicinal plant endemic to Algeria. Leaves decoction is used in folk medicine for anticancer, anti-inflammatory, and antidiabetic properties. Objective: Herein, the antioxidant and anti-inflammatory properties of different extracts from leaves and flowers of Algerian TM were evaluated. Materials and Methods: The study was carried out by in vitro cell-free assays (antioxidant/radical properties), ex vivo experiments (inhibition of prostaglandin E2 and thromboxane B2 release in human whole blood) and in vitro experiments on cell systems (cytotoxicity on peripheral blood mononuclear cells, and protective effects on human vein endothelial cells exposed to TNF-α). Results: The acetone TM extract showed significant antioxidant properties and excellent anti-inflammatory and cyclooxygenase-inhibitory activity, together with lack of toxicity on normal human blood cells; furthermore, it was able to protect endothelial cells against dysfunction induced by TNF-α, as shown by decrease in cell death, e-selectin expression and leukocyte adhesion. Conclusion: On these bases, TM leaves and flowers appear to be a good source of bioactive compounds with significant antioxidant and antiinflammatory capability, and potentially effective in prevention and treatment of pathological conditions related to oxidative stress and inflammation, such as endothelial dysfunction. SUMMARY Thymelaea microphylla leaves and flowers are a good source of bioactive compounds with significant antioxidant/free radical scavenger and anti-inflammatory activity. The acetone extract from leaves and flowers of Algerian Thymelaea microphylla possesses excellent cyclooxygenase-inhibitory activity. This extract is able to protect against endothelial dysfunction, an early event in development of atherosclerosis and vascular diseases. Abbreviations used: TM: Thymelaea microphylla; BCB: Beta-carotene bleaching; AcE: Acetone extract; PGE2: Prostaglandin E2; TxB2: Thromboxane B2; FL: Fluorescein; Cat: Catechin; DPPH: 2,2-diphenyl-1-picrylhydrazyl; ABTS: 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid)+; Que: Quercetin; ORAC: Oxygen radical absorbance capacity; AAPH: 2,2'-azobis (2-methylpropionamidine)dihydrochloride; PMS/NADH: Phenazine methosulfate/nicotinamide adenine dinucleotide; HUVECs: Human umbilical vein endothelial cells. Antonio Speciale
Functional inactivation of the SR family of splicing factors during a vaccinia virus infection

SR proteins are essential splicing factors required for constitutive splicing and function as key regulators of alternative RNA splicing. We have shown that SR proteins purified from late adenovirus‐infected cells (SR‐Ad) are functionally inactivated as splicing enhancer or splicing repressor proteins by a virus‐induced partial de‐phosphorylation. Here, we show that SR proteins purified from late vaccinia‐virus‐infected cells (SR‐VV) are also hypo‐phosphorylated and functionally inactivated as splicing regulatory proteins. We further show that incubating SR‐Ad proteins under conditions that restore the phospho‐epitopes to the SR proteins results in the restoration of their activity as splicing enhancer and splicing repressor proteins. Interestingly, re‐phosphorylation of SR‐VV proteins only partially restored the splicing enhancer or splicing repressor phenotype to the SR proteins. Collectively, our results suggest that viral control of SR protein activity may be a common strategy used by DNA viruses to take control of the host cell RNA splicing machinery.
Effects of 12-Week On-Field Combined Strength and Power Training on Physical Performance Among U-14 Young Soccer Players

Wong, P-L, Chamari, K, and Wisløff, U. Effects of 12-week on-field combined strength and power training on physical performance among U-14 young soccer players. J Strength Cond Res 24(3): 644-652, 2010-This study examined the effects of on-field combined strength and power training (CSPT) on physical performance among U-14 young soccer players. Players were assigned to experimental (EG, n = 28) and control groups (CG, n = 23). Both groups underwent preseason soccer training for 12 weeks. EG performed CSPT twice a week, which consisted of strength and power exercises that trained the major muscles of the core, upper, and lower body. CSPT significantly (p < 0.05) improved vertical jump height, ball-shooting speed, 10 m and 30 m sprint times, Yo-Yo intermittent endurance run (YYIER), and reduced submaximal running cost (RC). CSPT had moderate effect on vertical jump, ball-shooting, 30 m sprint, and YYIER, small effect on 10 m sprint, RC, and maximal oxygen uptake. YYIER had significant (p < 0.05) correlations with 10 m (r = −0.47) and 30 m (r = −0.43) sprint times, ball-shooting speed (r = 0.51), and vertical jump (r = 0.34). The CSPT can be performed together with soccer training with no concomitant interference on aerobic capacity and with improved explosive performances. In addition, it is suggested that CSPT be performed during the preseason period rather than in-season to avoid insufficient recovery/rest or overtraining.
LIFETIME INHALANT USE AMONG ALTERNATIVE HIGH SCHOOL STUDENTS IN TEXAS: PREVALENCE AND CHARACTERISTICS OF USERS

This cross-sectional study describes the prevalence of lifetime inhalant use and identifies demographic, psychosocial, and other risk behavior characteristics of students reporting lifetime inhalant use. The sample consisted of 354 students attending alternative high schools (dropout prevention/recovery schools) in Texas. The prevalence of lifetime inhalant use was 27.7%. After controlling for potentially confounding factors, students reporting lifetime inhalant use were less likely to be financially supported by their parents/guardians, more likely to use alcohol/tobacco, marijuana, and cocaine, and more likely to carry weapons and consider suicide. Study results may allow school officials, parents, researchers, and health care providers to gain a better understanding of inhalant use among students at risk for dropping out of school, a group which has not been extensively studied by previous researchers.
Building Robust Housing Sector Policy Using the Ecological Footprint

The vulnerability of the urban residential sector is likely to increase without the mitigation of growing household Ecological Footprints (energy demand, CO2 emissions, and demand for land). Analysis comparing the effectiveness and robustness of policy to mitigate the size of the housing Ecological Footprint has been limited. Here, we investigate three mitigation options: (1) reducing housing floor area, (2) improving the building envelope efficiency, and (3) reducing the carbon intensity of the electricity sector. We model the urban residential Ecological Footprint for a sub-national case study in Australia but analyse the results in the global context. We find that all three mitigation options reduce the Ecological Footprint. The success of policy to reduce household energy demand and land requirements is somewhat dependent on uncertain trajectories of future global population, affluence, and technological progress (together, global uncertainty). Carbon emissions reductions, however, are robust to such global uncertainty. By reducing the Ecological Footprint of the urban residential housing sector we see a reduction in its vulnerability to future global uncertainty, global carbon price, urban sprawl, and future energy shortages. Over the long term, such policy implementation can also be highly cost effective.
The relation between language regression and social communicative development of children with autism spectrum disorder.

PURPOSE To investigate the relationship between language regression and the subsequent social-communicative development of preschool children with Autism Spectrum Disorder (ASD). METHODS Thirty children with ASD participated in the study and were divided into two groups: with (n=6) and without (n=24) language regression. Language regression was assessed by the Autism Diagnostic Interview-Revised and the social-communicative development was measured by the Autism Diagnostic Observation Schedule. RESULTS Of the 30 children who met the criteria for participation in this study, six (20%) had regression of oral language skills, with a mean age of onset of 25 months. There were no statistical significant differences in the social-communicative development between the groups with and without language regression. CONCLUSION The findings of this research do not seem to confirm the relationship between the occurrence of language regression and the subsequent impairment on social-communicative development of children with ASD.
Anal sphincter EMG in the diagnosis of parkinsonian syndromes

Winge K, Jennum P, Lokkegaard A, Werdelin L. Anal sphincter EMG in the diagnosis of parkinsonian syndromes. Acta Neurol Scand: 2010: 121: 198–203. © 2009 The Authors Journal compilation © 2009 Blackwell Munksgaard.
Effects of Bisphosphonate Administration on Cleft Bone Graft in a Rat Model

Objective Bone grafts in patients with cleft lip and palate can undergo a significant amount of resorption. The aim of this study was to investigate the effects of bisphosphonates (BPs) on the success of bone grafts in rats. Design Thirty-five female 15-week-old Fischer F344 Inbred rats were divided into the following experimental groups, each receiving bone grafts to repair an intraoral CSD: (1) Graft/saline: systemic administration of saline and (2) systemic administration of zoledronic acid immediately following surgery (graft/BP/T0), (3) 1 week postoperatively (graft/BP/T1), and (4) 3 weeks postoperatively (graft/BP/T2). As an additional control, the defect was left empty without bone graft. Main Outcome Measures Microcomputed tomography and histologic analyses were performed in addition to evaluation of osteoclasts through tartrate-resistant acid phosphatase staining. Results Bone volume fraction (bone volume/tissue volume) for the delayed BP treatment groups (graft/BP/T1 = 45.4% ± 8.8%; graft/BP/T2 = 46.1% ± 12.4%) were significantly greater than that for the graft/saline group (31.0% ± 7.9%) and the graft/BP/T0 (27.6% ± 5.9%) 6 weeks postoperatively (P < .05). Hematoxylin and eosin staining confirmed an evident increase in bone volume and fusion of defect margins with existing palatal bone in the graft/BP/T1 and graft/BP/T2 groups. The graft/BP/T0 group showed the lowest bone volume with signs of acute inflammation. Conclusions Delayed BP administration following cleft bone graft surgery led to significant increase in bone volume and integration compared with saline controls. However, BP injection immediately after the surgery did not enhance bone volume, and rather, may negatively affect bone graft incorporation.
Is Implied Correlation Worth Calculating? Evidence from Foreign Exchange Options and Historical Data

Implied volatilities, as derived from option prices, have been shown to be useful in forecasting the subsequently observed volatility of the underlying financial variables. In this paper, we address the question of whether implied correlations, derived from options on the exchange rates in a currency trio, are useful in forecasting the observed correlations. We compare the forecast performance of the implied correlations from two currency trios with markedly different characteristics against correlation forecasts based on historical, time-series data. For the correlations in the USD/DEM/JPY currency trio, we find that implied correlations are useful in forecasting observed correlations, but they do not fully incorporate all the information in the historical data. For the correlations in the USD/DEM/CHF currency trio, implied correlations are much less useful. In general, since the performance of implied correlations varies across currency trios, implied correlations may not be worth calculating in all instances.
Muscle blood flow and mitochondrial function: influence of aging.

Skeletal muscle mitochondrial capacity (mito), tissue blood flow (BF) capacity, and oxygen exchange capacity (e.g., DO2) appear to be well matched. The different skeletal muscle fiber types and muscle remodeled, due to inactivity (e.g., related to aging or disease) or exercise training, exhibit widely differing aerobic capacities (VO2max). Yet, there are remarkably coordinated alterations in these 3 parameters in each of these conditions. With such a balance, there is likely shared control among these parameters in limiting VO2max of muscle, although this is a matter of considerable debate. The reduction in aerobic capacity in elderly can be improved by submaximal aerobic exercise training; this is related to increases in muscle mitochondria concentration and capillarity, but probably not BF capacity, as this is limited by central cardiovascular function. Thus, exercise-induced biochemical adaptations and angiogenesis occur in the elderly. The increase in muscle capillarity likely contributes to the increased oxygen exchange capacity, typical of endurance type training. The increase in [mito] appears essential to realize the increased in muscle VO2max with training and amplifies the rate-limiting influence of the muscles oxygen exchange capacity. Further, vascular remodeling induced by exercise in the elderly could be effective at improving flow capacity, if limited by peripheral obstruction. Thus, the limits to aerobic function specific to aged muscle appear most influenced by inactivity, whereas central cardiovascular changes impact whole body performance. Some may consider the aged myocyte as a small, inactive, normal myocyte in need of activity!
Inactivation of Semliki Forest Virus in aerosols

Purified Semliki forest virus in aerosols is inactivated rapidly at 40% and above 70% relative humidity. At all humidities tested the decay of virus infectivity runs parallel with the decrease in hemagglutination activity, whereas the biological integrity of the virus ribonucleic acid is preserved. Also, free infectious ribonucleic acid is stable after spraying at all relative humidities. Evidence is presented for the hypothesis that above 20% relative humidity, virus inactivation in aersols is mainly due to surface-dependent factors, damaging the virus coat.
Using diffusion of innovations theory to guide diabetes management program development: an illustrative example.

BACKGROUND Optimal management of type 2 diabetes requires achievement of optimal glucose, blood pressure and lipid targets through promotion of prudent diet, regular physical activity and adherence to necessary medication. This may require the development of new programs for the coordination of required multidisciplinary services. Diffusion of innovations theory offers a conceptual framework that may facilitate the implementation of such programs. METHODS To illustrate this, we have re-examined the implementation experiences previously reported by the developers of an actual diabetes management pilot program in Montreal, with an eye toward identifying potentially important process factors that could effectively increase adoption and sustainability. RESULTS Physician participation in the program appeared to be influenced by perceived advantages of participation, compatibility of the program with own perspective and perceived barriers to participation. Organizational features that may have influenced participation included the extent of the program's integration within the existing health care system. CONCLUSION A thorough consideration of process factors that impact system and team integration must equally include a focus on ensuring ongoing partnerships among the producers of the model, governments, nongovernmental organizations, private industry, user professionals and patients. This can only be achieved when a knowledge transfer action plan is developed to guide program development, implementation and sustainability.
Escape from Working Poverty: Steps toward Sustainable Livelihood

Working poverty affects over half the world’s working population, yet we know remarkably little about the role of wages in transitioning toward sustainable livelihood. We develop and test a model whereby as pay approaches a living wage range, pay fairness becomes clearly associated with work–life balance; this in turn links to job satisfaction, which is a four-step process at the psychological level. We further extend this by testing a moderated mediated model, whereby income level is tested as a boundary condition. Using data from N = 873 New Zealand employees, we focus on relatively low-waged employees across three levels of income: up to $20,000, $20–40,000, and $40–60,000, with the last band straddling the New Zealand Living Wage. We find strong support for pay fairness predicting work–life balance and job satisfaction, with work–life balance mediating the relationship toward job satisfaction. In addition, we find direct effects from income to work–life balance, although not job satisfaction. Furthermore, two-way moderation is supported toward work–life balance and job satisfaction, with higher income employees reporting higher outcomes when fairness is high. The index of moderated mediation is also significantly supporting, indicating that work–life balance has a stronger mediation effect as income rises. Thus, as workers emerged from working poverty, pay fairness, and in turn work–life balance, became psychologically more salient for happiness at work, implying that a pathway to Sustainable Development Goal 8 includes at least three psychological steps, in addition to the pecuniary issue of pay: fairness, work–life balance, and job satisfaction.
Portal vein embolization with steel coils and absolute ethanol: A comparative experimental study with canine liver

Portal vein embolization (PVE) is expected to have an adjuvant effect on the treatment of human liver tumors with transcatheter hepatic arterial embolization (TAE). In this experimental study, PVE was tried using two kinds of embolic materials, steel coils (coils) and absolute ethanol (ethanol). The aim of this article is to compare the effectiveness between the two methods. Five dogs and 12 dogs were treated with coils and ethanol, respectively. The second order portal branches were embolized. Embolization effects of each embolic material on the hepatic vessels and the liver parenchyma were studied angiographically and histologically. PVE with coils achieved immediate occlusion of the embolized vessels in all dogs. Unexpectedly, unembolized vessels were also constricted and/or obstructed in 4 of 5 dogs during a 4‐week follow‐up period. Histological examination showed no hepatic necrosis in all dogs. By contrast, in the dogs treated with ethanol, venous occlusion was achieved immediately after embolization with ethanol of 0.4 mL/kg or more. The embolized vessels remained occluded and no obstruction of unembolized vessels was observed until 4 weeks after embolization. Hepatic necrosis was observed in the segments where portal veins were occluded. The degree of parenchymal damage was proportional to the injected ethanol dose. Judging from these results, ethanol is likely to be more suitable than coils as an embolic material for PVE. PVE with coils is often dangerous because unembolized vessels might be occluded unexpectedly. For the clinical use of PVE with thanol, the appropriate dose of injection should be investigated. (Hepatology 1995; 22:1812‐1818).