Miracle-dz/UniFAR-FTUs huggingface dataset:
Post-traumatic stress disorder and depression

Background We examined psychiatric morbidity following war-related psychic trauma, with a special focus on the depressive comorbidity of post-traumatic stress disorder (PTSD). Method Subjects consisted of 60 Israeli veterans who sought psychiatric treatment 4–6 years after having been exposed to war trauma. PTSD and psychiatric comorbidity were diagnosed using the Structured Interview for PTSD and the Schedule for Affective Disorders and Schizophrenia. Results Both lifetime (100%) and current (87%) PTSD were the most prevalent disorders. Comorbidity was extensive, with major depressive disorder (MDD) most prevalent (95% lifetime, 50% current), followed by anxiety disorders, minor affective disorders, and alcoholism or drug misuse. Conclusions Within post-traumatic psychiatric morbidity of combat origin, PTSD and MDD are the most prevalent disorders. In addition it appears that PTSD, although related to post-traumatic MDD beyond a mere sharing of common symptoms, is of the same time differentiated from it as an independent diagnostic category.
Similarities and differences in outcomes of cirrhosis due to nonalcoholic steatohepatitis and hepatitis C

The objective of this study was to prospectively define outcomes of cirrhosis due to nonalcoholic steatohepatitis (NASH) and compare them with those associated with hepatitis C virus (HCV) infection. We compared 152 patients with cirrhosis due to NASH with 150 matched patients with cirrhosis due to HCV. Over 10 years, 29/152 patients with cirrhosis due to NASH died compared with 44/150 patients with HCV (P < .04). This was mainly due to the lower mortality rate in patients with Child class A cirrhosis due to NASH versus HCV (3/74 vs. 15/75; P < .004). There were no significant across‐group differences in mortality in patients with Child class B or C cirrhosis. Sepsis was the most common cause of death in both groups; patients with NASH had a higher cardiac mortality (8/152 vs. 1/150; P < .03). Patients with Child class A cirrhosis due to NASH also had a significantly lower risk of decompensation, defined by a 2‐point increase in Child‐Turcotte‐Pugh score (P < .007). Cirrhosis due to NASH was associated with a lower rate of development of ascites (14/101 vs. 40/97 patients at risk; P < .006). NASH also had a significantly lower risk of development of hepatocellular carcinoma (10/149 vs. 25/147 patients at risk; P < .01). In conclusion, compensated cirrhosis due to NASH is associated with a lower mortality rate compared with that due to HCV. It is also associated with a lower rate of development of ascites, hyperbilirubinemia, and hepatocellular carcinoma. However, cardiovascular mortality is greater in patients with NASH. (HEPATOLOGY 2006.)
Role of interferon-α and interferon-β in glomerular injury and repair

Obwohl die immunstimulatorischen Effekte viraler Nukleinsaursen, wie auch IFN -α und IFN-β, wahrend Virusinfektionen eine wichtige Rolle spielen, ist wenig uber ihre Funktion bei viraler Glomerulonephritis, wie beispielsweise HIV Nephropathie, bekannt. Virusinfektionen aktivieren, vor allem mittels IFN-α und IFN-β Produktion eine systemische antivirale Immunantwort. Es wurde gezeigt, dass diese inflammatorischen Zytokine einen pleiotropen immunmodulatorischen Effekt auf renale Mesangialzellen ausuben, was direkt zu glomerularen Krankheiten fuhrt. Aber es ist bisher nicht bekannt, ob die viralen Nukleinsauren und Typ I IFN einen Effekt auf die glomerularen Epithelzellen haben. (z.B. Podozyten und PECs). Um den Effekt von Nukleinsauren auf Podozyten und PECs zu erforschen, stimulierten wir diese Zellen mit synthetischen dsDNA-(poly-dAdT) Komplexen mit lipofectamine, um eine virale Infektion zu imitieren. Wir haben herausgefunden, dass dsDNA stetig viele IFN-stimulierte Gene in Podozyten und PECs induziert. Desweitern haben wir herausgefunden, dass dsDNA die PECs Proliferation mindert und die CD24+/CD133+PECs Differenzierung zu ausgereiften Podozyten inhibiert. Um unsere Hypothese, dass deis aufgrund von der Sekretion von IFN-α und IFN-β passiert ist, zu bestatigen, haben wir den Effekt von diesen anitviralen Zytokinen auf PECs- und Podozyten-Homoostase etabliert. Wir haben herausgefunden, dass beide IFNs stetig Podozyten und PECs dazu anregen, stetig mehrere IFN-stimulierte Gene zu exprimieren. Trotzdem hat nur IFN-β das Podozytensterben induziert und die Permeabilitat der Podozyten-Monolayer erhoht. In der Adriamycin-induzierter Nephropathie bei SCID Mausen haben Injektionen mit IFN-α oder IFN-β die Proteinurie, den Makrophagen Influx und die Glomerulosklerose verstarkt. Trotzdem induziert nur IFN-β das mitotische Podozytensterben (katastrophale Mitose), welches zu einer reduzierten Podozytenanzahl fuhrt. Wir haben fuhrt, dass IFN-α einen Zellzyklusarrest in-vivo bei PECs induziert, der zur glomerularen Schadigung fuhrt. Balb/c Mause, die Adriamycin gespritzt bekommen haben und taglich mit IFN-α und IFN-β behandelt wurden zeigten einen aggravierten Phanotyp mit vermehrter Proteinurie. Im Gegensatz zu dem, was an Studien in SCID Mausen gezeigt wurde, war der Effekt auf die Proteinurie nach IFN-α Behandlung prominenter bei Balb/c Mausen, verglichen mit IFN-β. Deshalb haben Typ I IFNs einen deutlichen Effekt auf Podozyten und Parietalzellen. Zusammen fordern die Typ I IFNs die Glomerulosklerose durch verstarkten Untergang der Podozyten sowie durch Unterdruckung ihrer Regeneration aus Vorlauferzellen.
Nuclear translocation of extracellular signal-regulated kinases in neuronal excitotoxicity

Subcellular distributions of extracellular signal-kinases (ERK1/2), including their activated form (p-ERK1/2), were investigated in glutamate-induced apoptotic-like death in cultured rat cortical neurons by Western immunoblot and immunocytochemistry. During 15 min glutamate exposure, p-ERK1/2 was increased in both cytosol and nuclear extracts, but prominently so in nuclear extracts. Simultaneously, ERK1/2 were mildly decreased in cytosol (to 0.7-fold vs sham control), largely increased in nuclear extracts (to 6.2-fold vs sham control), but not changed in total cell extracts. Immunocytochemistry studies also showed a large increase in nuclear and a mild decrease in cytosol extracts of ERK1/2 at 15 min of exposure. After glutamate exposure, all the above changes reverted simultaneously. The nuclear increase of ERK1/2 was largely prevented by inhibition of ERK1/2 activation, but prolonged by elongation of ERK1/2 activation. These observations suggest that stimulation of glutamate receptors in cortical neurons may incur an activation-dependent transient nuclear translocation of ERK1/2, which might be involved in excitotoxicity through a simultaneous strong elevation of p-ERK1/2 in nucleus.
Comparison between 3 infusion methods to measure cerebrospinal fluid outflow conductance.

OBJECT There are several infusion methods available to estimate the outflow conductance (Cout) or outflow resistance (Rout=1/Cout) of the CSF system. It has been stated that for unknown reasons, the bolus infusion method estimates a higher C(out) than steady-state infusion methods. The aim of this study was to compare different infusion methods for estimation of Cout. METHODS The following 3 different infusion methods were used: the bolus infusion method (Cout bol); the constant flow infusion method, both static (Cout stat) and dynamic (Cout dyn) analyses; and the constant pressure infusion method (Cout cpi). Repeated investigations were performed on an experimental model with well-known characteristics, with and without physiological pressure variations (B-waves, breathing, and so on). All 3 methods were also performed in a randomized order during the same investigation in 20 patients with probable or possible idiopathic normal-pressure hydrocephalus; 6 of these patients had a shunt and 14 did not. RESULTS Without the presence of physiological pressure variations, the concordance in the experimental model was good between all methods. When they were added, the repeatability was better for the steady-state methods and a significantly higher Cout was found with the bolus method in the region of clinically relevant Cout (p<0.05). The visual fit for the bolus infusion was dependent on subjective assessment by the operator. This experimental finding was confirmed by the clinical results, where significant differences were found in the investigations in patients without shunts between Cout of the visual bolus method and Cout stat, Cout dyn, and Cout cpi (4.58, 4.18, and 6.12 μl/[second×kPa], respectively). CONCLUSIONS This study emphasized the necessity for standardization of Cout measurements. An experienced operator could partly compensate for difficulties in correctly estimating the pressure parameters for the bolus infusion method, but for the general user this study suggests a steady-state method for estimating Cout.
Two hsp23 genes in the Mediterranean fruit fly, Ceratitis capitata: structural characterization, heat shock regulation and developmental expression

In the present study, we characterized a 3320‐bp genomic DNA fragment encoding two medfly (Ceratitis capitata) homologues of the Drosophila melanogaster heat shock protein 23 (hsp23) gene, named Cchsp23‐αand ‐β. The two medfly hsp23 genes are transcribed in opposite directions and encode two almost identical proteins. Furthermore, the two genes exhibit a very high degree of similarity in their 5′ untranslated and proximal promoter regions. Phylogenetic analysis indicated that the CcHsp23 proteins are orthologous to Drosophila Hsp23 and Sarcophaga crassipalpis Hsp23. Structural analysis of the 5′ flanking regions of the Cchsp23 genes revealed the presence of several putative heat shock elements. Both CcHsp23 genes are induced by heat in a similar manner. In addition to heat‐induction, the Cchsp23 genes are expressed at several stages of normal development as well as in ovaries and testes. In general, the developmental expression patterns of the medfly genes are similar, suggesting that they are under similar regulatory mechanisms. However, the expression of the Cchsp23 genes differs significantly from the expression of the Drosophila hsp23 gene in certain embryonic and larval stages, suggesting differential regulation of the hsp23 genes in the two dipteran species. The expression of both Cchsp23 genes in adult flies is increased with age, especially in males.
Measurement Error in Earnings Data: Using a Mixture Model Approach to Combine Survey and Register Data

Survey data on earnings tend to contain measurement error. Administrative data are superior in principle, but are worthless in case of a mismatch. We develop methods for prediction in mixture factor analysis models that combine both data sources to arrive at a single earnings figure. We apply the methods to a Swedish data set. Our results show that register earnings data perform poorly if there is a (small) probability of a mismatch. Survey earnings data are more reliable, despite their measurement error. Predictors that combine both and take conditional class probabilities into account outperform all other predictors. This article has supplementary material online.
Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D)

Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abequose and paratose synthase genes: rfbJ of Salmonella serogroup B, rfbJ of Salmonella serogroup C2, and rfbS of Salmonella serogroup D (also present in serogroup A). For good differentiation among these major serogroups, the primers were designed not only to give precise specificity in priming but also to give DNA products with different sizes in polymerase chain reactions (product sizes, approximately 720 bp for both serogroups A and D, approximately 820 bp for serogroup C2, and approximately 882 bp for serogroup B). In a polymerase chain reaction assay utilizing these rfb-specific primers, all of the 40 salmonellae belonging to serogroups B, C2, and D plus A were accurately identified among a total of 123 clinical isolates tested (including 55 salmonellae from 36 different serotypes and 68 strains from 10 other members of the family Enterobacteriaceae). No false-positive reactions were detected. The selected rfb gene sequences were proved for the first time to be useful DNA-based markers for identification of and differentiation among Salmonella serogroups A, B, C2, and D.
The Association Between XPG Gene Polymorphism and Gastric Cancer Risk.

PURPOSE Studies exploring the association between the Xeroderma pigmentosum group G (XPG) gene polymorphisms and gastric cancer (GC) risk provide conflicting findings. Thus, this meta-analysis was performed. MATERIALS AND METHODS The PubMed and EMBASE databases were comprehensively searched to identify studies for the inclusion in the meta-analysis. The strength of the association was evaluated by calculating pooled odds ratios and 95% confidence intervals. RESULTS Nine case-control studies involving 3540 cases and 3953 controls were included in the meta-analysis, which revealed that the XPG rs751402 polymorphism is positively associated with GC risk and could be viewed as a risk factor of GC in three genetic models. CONCLUSION The XPG gene rs751402 polymorphism is associated with an increased risk of GC in Chinese Han populations. This finding should be verified by larger studies that include additional ethnic groups.
Biochemical analysis of a Ca2+‐dependent membrane–membrane interaction mediated by the sea urchin yolk granule protein, toposome

Toposome, a high molecular mass protein, is an abundant component of the yolk granule in the sea urchin egg and embryo. Toposome is composed of a 160 kDa polypeptide that is proteolytically processed into smaller species of 120 and 90 kDa during embryonic development. The exact biological function of toposome during early development is unknown. In this study we have examined calcium binding to toposome and the effect of this binding on the secondary and tertiary structural characteristics of the purified protein. Initially, we used equilibrium dialysis to quantify calcium binding to toposome. Monophasic binding of up to 600 M of calcium per mole of protein was detected with an intrinsic dissociation constant (calcium) of 240 µm. Increasing concentrations of calcium resulted in an increase in alpha helical content from 3.0 to 22.0%, which occurred with an apparent dissociation constant (calcium) of 25 µm. In parallel experiments, toposome binding to liposomes required similar concentrations of calcium; an apparent dissociation constant (calcium) of 25 µm was recorded. Endogenous tryptophan fluorescence measurements, both in the presence and absence of liposomes, demonstrated that the tertiary structure is sensitive to increasing concentrations of calcium with an apparent dissociation constant (calcium) of 240 µm. Toposome‐driven, liposome aggregation assays revealed a similar calcium requirement. Collectively, these results define a two‐step model for calcium modulation of toposome structure and function.
Cytogenetic mapping of 31 functional genes on chicken chromosomes by direct R-banding FISH

Using direct R-banding fluorescence in situ hybridization, we determined the location of 31 functional genes on chicken chromosomes. Replication R-banded chromosomes were obtained by synchronizing splenocyte cultures with excessive thymidine, followed by BrdU treatment. Thirty-one functional genes were directly localized to banded chicken chromosomes using genomic DNA and cDNA fragments as probes. The possibility of conserved linkage homology between chicken and human chromosomes was demonstrated for seven chicken chromosome regions (1p, 1q, 2q, 4p, 4q, and 5q).
Human Papillomavirus Prevalence and Type Distribution in Male Anogenital Sites and Semen

Background: Human papillomavirus (HPV) is sexually transmitted and causes cervical cancer. Although HPV can infect men and women, little is known about infection in men. Specifically, the prevalence of type-specific HPV infection and the distribution of infections by anogenital anatomic site in men are incompletely characterized. Methods: We tested 463 men ages 18 to 40 years for HPV at the glans/corona, penile shaft, scrotum, urethra, perianal area, anal canal, and in a semen sample. Eligible men acknowledged no history of genital warts and had sexual intercourse with a woman within the past year. HPV testing by PCR and reverse line blot genotyping for 37 types was conducted on each of the specimens from the seven sampling sites. Results: When HPV results from any sampling site were considered, 237 (51.2%) men were positive for at least one oncogenic or nononcogenic HPV type, and another 66 (14.3%) men were positive for an unclassified HPV type. The types with the highest prevalence were HPV-16 (11.4%) and 84 (10.6%). External genital samples (glans/corona, shaft, and scrotum) were more likely than anal samples to contain oncogenic HPV (25.1% versus 5.0%). HPV-positive penile shaft and glans/corona samples were also more likely to be infected with multiple HPV types than other sites. Conclusions: More complete anogenital sampling and sensitive detection for 37 HPV types resulted in a higher HPV prevalence in primarily asymptomatic men than reported previously. The penile shaft was the site most likely to be HPV positive and harbored the greatest proportion of multiple type and oncogenic infections. These results have implications for research of HPV among men and transmission between partners. (Cancer Epidemiol Biomarkers Prev 2007;16(6):1107–14)
A meta-analysis of host specificity in Neotropical hard ticks (Acari: Ixodidae)

Abstract Host specificity of Neotropical hard ticks (Acari: Ixodidae) was analyzed by using the number of hosts species for each tick species and the index of host specificity STD*, which integrates phylogenetic and ecological information. The analyses were based on 4172 records of hard ticks collected from wild and domestic tetrapods. Most tick species included in this study were associated with three to 20 host species. No tick species has been associated either with a single species or with a single genus of host. It was found that the number of host species is sensitive to sampling effort, but not the STD*. The most frequent values of STD* were between 2.5 and 3.5, which shows that the host species more frequently used by Neotropical hard tick species belong to different families or different orders. Immature stages tend to use a broader taxonomic range of hosts than adults, and the interpretation of both measures of host specificity used in this study led to the conclusion that the impact of non-endemic hosts does not alter the patterns of host specificity in Neotropical hard ticks. The index STD* showed that a high proportion of tick species has phylogenetically unrelated species as principal hosts. The conclusion reached in this work indicates that strict host specificity is not common among Neotropical hard ticks and suggests that the influence of tick ecology and evolution of habitat specificity, tick generation time, phenology, time spent off the host and the type of life-cycle could be more important than hosts species.
Reintegration of Pakistani return migrants from the Middle East in the domestic labour market.

This study compared the unemployment rates among return migrants and nonmigrants and examined the reintegration pattern of returnees in the domestic labor market. The study utilized three data sets: the 1980 World Bank Survey of Return Migrant Households; the 1986 ILO/ARTEP Survey of Return Migrant Households; the 1991 Pakistan Integrated Household Survey. Findings showed that unemployment rates were much higher among return migrants than nonmigrants. Although this difference narrowed with the passage of time, even among those who returned to Pakistan at least 18 months prior to the surveys, more than 10% of workers were unemployed. The multivariate analysis further showed that returnees, irrespective of the period elapsed since their return, were more likely to be unemployed than nonmigrants. With respect to the reintegration pattern of return migrants, the study revealed that variables indicating their human capital, such as occupation and pre-migration and during-migration work experience, appear to have a greater influence on their post-return adjustment than the variables related to economic positions such as savings. The results also showed that the types of jobs unemployed returnees were looking for differed substantially from those held by employed return migrants. A possibility was that unemployed returnees could not save enough from their overseas earnings to become self-employed. Thus, provision of credit for self-employment seems to be the right way to accommodate these workers.
La migración internacional en América Latina y el Caribe: tendencias y perfiles de los migrantes

Resumen En este trabajo se describe la evolucion de la migracion internacional de ciudadanos latinoamericanos y caribenos durante las ultimas decadas. El primer capitulo se refiere al contexto internacional en el que se insertan los procesos migratorios, destacando los factores de diversa indole que los han constituido en una preocupacion creciente. Luego, se examinan las grandes tendencias de la migracion internacional en America Latina y el Caribe, tanto en su dimension intrarregional como hacia los Estados Unidos, relacionandolas, de modo general, con las especificidades del desarrollo social y economico. A continuacion se presentan las caracteristicas de los migrantes latinoamericanos y caribenos, prestando atencion a su heterogeneidad educativa y ocupacional, a fin de identificar sus condiciones de insercion en los principales paises de destino. Esta caracterizacion se utiliza tambien para examinar las repercusiones socioeconomicas de la emigracion en los paises de origen y su impacto sobre el desarrollo de esos paises, y se destaca la coexistencia de potencialidades positivas y adversidades, entre otras, las de las remesas. Se concluye con algunas reflexiones tendientes a formular hipotesis sobre el futuro de los movimientos migratorios inter-nacionales en la region, revisandose los supuestos socioeconomicos y sociodemograficos sobre los que se apoyan tales hipotesis. En un anexo, se ofrece un breve examen de las fuentes de informacion para el analisis de la migracion internacional, con especial referencia a la situacion existente.