Miracle-dz/UniFAR-FTUs huggingface dataset:
Activation of signal transducer and activator of transcription 3 (Stat3) pathway in osteosarcoma cells and overexpression of phosphorylated‐Stat3 correlates with poor prognosis

Stat3 expression in cancer may have important prognostic and therapeutic value, but there has been no reports correlating Stat3 expression with prognosis in patients with osteosarcoma. The goal of this study is to correlate patient prognosis with the expression of Stat3 in osteosarcoma tissue and determine the effectiveness of blocking this pathway in osteosarcoma cell lines by Stat3 inhibitor, CDDO‐Me. We examine the expression levels of Stat3 and pStat3 in osteosarcoma cell lines and primary tissues by Western blot analysis. We also evaluate the levels of pStat3 expression in osteosarcoma tissue microarray (TMA) by immunohistochemistry. We use clinical data to determine the impact of levels of Stat3 expression on patient prognosis. Finally, we evaluated the effect of CDDO‐Me on the inhibition of activated Stat3 pathway in osteosarcoma cell lines using MTT assay and Western blot analysis. Stat3 is observed to be activated in osteosarcoma tissues as well as in cultured cell lines. Overexpression of pStat3 is associated with poor prognosis. CDDO‐Me inhibits the growth of osteosarcoma cell lines and induces apoptosis as well. Our results suggest that Stat3 may be a prognostic indicator and potential therapeutic target for osteosarcoma. Blocking the pathway of Stat3 may lead to develop new therapeutic strategies against osteosarcoma. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:971–978, 2010
Poliovirus excretion among persons with primary immune deficiency disorders: summary of a seven-country study series.

BACKGROUND Persons with primary immune deficiency disorders (PID), especially those disorders affecting the B-cell system, are at substantially increased risk of paralytic poliomyelitis and can excrete poliovirus chronically. However, the risk of prolonged or chronic excretion is not well characterized in developing countries. We present a summary of a country study series on poliovirus excretion among PID cases. METHODS Cases with PID from participating institutions were enrolled during the first year and after obtaining informed consent were tested for polioviruses in stool samples. Those cases excreting poliovirus were followed on a monthly basis during the second year until 2 negative stool samples were obtained. RESULTS A total of 562 cases were enrolled in Bangladesh, China, Iran, Philippines, Russia, Sri Lanka, and Tunisia during 2008-2013. Of these, 17 (3%) shed poliovirus, including 2 cases with immunodeficient vaccine-derived poliovirus. Poliovirus was detected in a single sample from 5/17 (29%) cases. One case excreted for more than 6 months. None of the cases developed paralysis during the study period. CONCLUSIONS Chronic polioviruses excretion remains a rare event even among individuals with PID. Nevertheless, because these individuals were not paralyzed they would have been missed by current surveillance; therefore, surveillance for polioviruses among PID should be established.
Personality characteristics of women before and after recovery from an eating disorder

Background. Previous studies of personality characteristics in women with eating disorders primarily have focused on women who are acutely ill. This study compares personality characteristics among women who are ill with eating disorders, recovered from eating disorders, and those without eating or other Axis I disorder pathology. Method. Female participants were assessed for personality characteristics using the Temperament and Character Inventory (TCI): 122 with anorexia nervosa (AN; 77 ill, 45 recovered), 279 with bulimia nervosa (BN; 194 ill, 85 recovered), 267 with lifetime histories of both anorexia and bulimia nervosa (AN+BN; 194 ill, 73 recovered), 63 with eating disorder not otherwise specified (EDNOS; 31 ill, 32 recovered), and 507 without eating or Axis I disorder pathology. Results. Women ill with all types of eating disorders exhibited several TCI score differences from control women, particularly in the areas of novelty-seeking, harm avoidance, self-directedness, and cooperativeness. Interestingly, women recovered from eating disorders reported higher levels of harm avoidance and lower self-directedness and cooperativeness scores than did normal control women. Conclusions. Women with eating disorders in both the ill and recovered state show higher levels of harm avoidance and lower self-directedness and cooperativeness scores than normal control women. Although findings suggest that disturbances may be trait-related and contribute to the disorders' pathogenesis, additional research with more representative community controls, rather than our pre-screened, normal controls, is needed to confirm these impressions.
Genetic and phenotypic variation across a hybrid zone between ecologically divergent tree squirrels (Tamiasciurus)

A hybrid zone along an environmental gradient should contain a clinal pattern of genetic and phenotypic variation. This occurs because divergent selection in the two parental habitats is typically strong enough to overcome the homogenizing effects of gene flow across the environmental transition. We studied hybridization between two parapatric tree squirrels (Tamiasciurus spp.) across a forest gradient over which the two species vary in coloration, cranial morphology and body size. We sampled 397 individuals at 29 locations across a 600‐km transect to seek genetic evidence for hybridization; upon confirming hybridization, we examined levels of genetic admixture in relation to maintenance of phenotypic divergence despite potentially homogenizing gene flow. Applying population assignment analyses to microsatellite data, we found that Tamiasciurus douglasii and T. hudsonicus form two distinct genetic clusters but also hybridize, mostly within transitional forest habitat. Overall, based on this nuclear analysis, 48% of the specimens were characterized as T. douglasii, 9% as hybrids and 43% as T. hudsonicus. Hybrids appeared to be reproductively viable, as evidenced by the presence of later‐generation hybrid genotypes. Observed clines in ecologically important phenotypic traits—fur coloration and cranial morphology—were sharper than the cline of putatively neutral mtDNA, which suggests that divergent selection may maintain phenotypic distinctiveness. The relatively recent divergence of these two species (probably late Pleistocene), apparent lack of prezygotic isolating mechanisms and geographic coincidence of cline centres for both genetic and phenotypic variation suggest that environmental factors play a large role in maintaining the distinctiveness of these two species across the hybrid zone.
Strain Analysis by Tissue Doppler Imaging: Comparison of Conventional Manual Measurement with a Semiautomated Approach

Myocardial strain and strain rate (SR) can be derived from either tissue Doppler (TDI) information or two‐dimensional speckle tracking. As conventional TDI analysis (TDI‐manual) is time‐consuming with poor reproducibility, we developed a faster semiautomated approach (TDI‐ST). We aimed to study the comparability of TDI‐ST with TDI‐manual, an established method for measuring strain and SR.
Biogeochemical Evolution of Cryoconite Holes on Canada Glacier, Taylor Valley, Antarctica

[1] The cryoconite holes of the McMurdo Dry Valleys are simple, closed biogeochemical systems involving water, ice, mineral and organic debris, which serve as ecosystems for consortia of microorganisms. This study is the first to document the seasonal and annual chemical evolution of solutes in cryoconite holes. Samples of glacier ice, frozen cryoconite holes and those containing water were collected during the austral summer of 2005–2006. The isolation age was calculated from the excess Cl− in the holes, and varied from 0 to 5 years (a), consistent with the last hot summer when the cryoconite holes were open to the atmosphere. The holes progressively deepen with isolation age. Variations in DIC, DOC, K+ and SO42− suggest that dissolution of primary minerals in the cryoconite debris, cyclical precipitation and dissolution of secondary carbonates, net photosynthesis over summer and net respiration during the autumnal freeze are the principal reactions which perturb the seasonal and annual solute concentrations in the holes. DOC is generated in the holes at a rate of 7.5 μg C cm−2 a−1, and non-sea-salt K+ accumulates in frozen holes at a rate of 0.073 μeq cm−2 a−1. We infer that C cycling is complex even in these otherwise simple systems.
Synthesis and Optical Properties of Near-Infrared meso-Phenyl-Substituted Symmetric Heptamethine Cyanine Dyes

Heptamethine cyanine dyes are a class of near infrared fluorescence (NIRF) probes of great interest in bioanalytical and imaging applications due to their modifiability, allowing them to be tailored for particular applications. Generally, modifications at the meso-position of these dyes are achieved through Suzuki-Miyaura C-C coupling and SRN1 nucleophilic substitution of the chlorine atom at the meso-position of the dye. Herein, a series of 15 meso phenyl-substituted heptamethine cyanines was synthesized utilizing a modified dianil linker. Their optical properties, including molar absorptivity, fluorescence, Stokes shift, and quantum yield were measured. The HSA binding affinities of two representative compounds were measured and compared to that of a series of trimethine cyanines previously synthesized by our lab. The results indicate that the binding of these compounds to HSA is not only dependent on hydrophobicity, but may also be dependent on steric interferences in the binding site and structural dynamics of the NIRF compounds.
Scaling Social Movements Through Social Media: The Case of Black Lives Matter

In this article, we explore the potential role of social media in helping movements expand and/or strengthen themselves internally, processes we refer to as scaling up. Drawing on a case study of Black Lives Matter (BLM) that includes both analysis of public social media accounts and interviews with BLM groups, we highlight possibilities created by social media for building connections, mobilizing participants and tangible resources, coalition building, and amplifying alternative narratives. We also discuss challenges and risks associated with using social media as a platform for scaling up. Our analysis suggests that while benefits of social media use outweigh its risks, careful management of online media platforms is necessary to mitigate concrete, physical risks that social media can create for activists.
Role of caspase‐9 in the effector caspases and genome expressions, and growth of bovine skeletal myoblasts

Caspase‐9 has been reported as the key regulator of apoptosis, however, its role in skeletal myoblast development and molecular involvements during cell growth still remains unknown. The current study aimed to present the key role of caspase‐9 in the expressions of apoptotic caspases and genome, and cell viability during myoblast growth using RNA interference mediated silencing. Three small interference RNA sequences (siRNAs) targeting caspase‐9 gene was designed and ligated into pSilencer plasmid vector to construct shRNA expression constructs. Cells were transfected with the constructs for 48 h. Results indicated that all three siRNAs could silence the caspase‐9 mRNA expression significantly. Particularly, the mRNA expression level of caspase‐9 in the cells transfected by shRNA1, shRNA2 and shRNA3 constructs were reduced by 37.85%, 68.20% and 58.14%, respectively. Suppression of caspase‐9 led to the significant increases in the mRNA and protein expressions of effector caspase‐3, whereas the reduction in mRNA and protein expressions of caspase‐7. The microarray results showed that the suppression of caspase‐9 resulted in significant upregulations of cell proliferation‐, adhesion‐, growth‐, development‐ and division‐regulating genes, whereas the reduction in the expressions of cell death program‐ and stress response‐regulating genes. Furthermore, cell viability was significantly increased following the transfection. These data suggest that caspase‐9 could play an important role in the control of cell growth, and knockdown of caspase‐9 may have genuine potential in the treatment of skeletal muscle atrophy.
Intertemporal Trading Ratios for Nutrient Pollution Control

There is signiﬁcant interest in the use of market mechanisms for controlling nutrient pollution, one of the most challenging threats to water quality in the United States and elsewhere. This type of pollution often is characterized by considerable lag times between discharge from the pollution source and delivery to the impaired waters. We investigate the implications of these lags for eﬃcient pollution reduction markets and compare two alternative market designs: 1) forward markets where participants trade pollution deliveries directly and 2) a trading ratio system where they trade contemporaneous discharges. While a system of ﬁrst-best trade ratios is complex in early periods, this system can produce the optimal steady state loads using a simple trading rule. We also ﬁnd that while ﬁrst-best trade ratios are greater than one when there are lag disparities between trading partners, second-best trade ratios under the same lag disparities may be less than one when the overall cap on discharges is set suﬃciently small.
Do smoking attitudes predict behaviour? A longitudinal study on the bi-directional relations between adolescents' smoking attitudes and behaviours.

AIMS Prevention and intervention programmes focus frequently upon retaining or creating negative attitudes towards smoking in an effort to prevent adolescents from smoking. As the focus upon attitudes is central in these programmes it is essential to know whether smoking attitudes actually precede smoking behaviour or, conversely, are affected by it. Therefore, in the present study we examined to what extent bi-directional relations existed between smoking attitudes and behaviour. DESIGN Data were used from the three annual waves of the 'Family and Health' project. SETTING Participants were asked to complete questionnaires individually at their homes. PARTICIPANTS Addresses of families consisting of two parents and two adolescents were obtained from the records of 22 municipalities in the Netherlands. At baseline, 428 families participated with a response rate of 94% at the third measurement. MEASUREMENTS Self-reports were used to assess adolescents' smoking attitudes and behaviour. Associations between smoking attitudes and behaviour were tested using structural equation modelling. FINDINGS Findings revealed that smoking attitudes did not predict smoking consistently over time. However, past smoking affected subsequent attitudes moderately, suggesting that adolescents who started to smoke developed less negative attitudes towards smoking. CONCLUSIONS The current findings imply that smoking behaviour predominantly shapes smoking-related attitudes, rather than vice versa. Focusing merely on smoking attitudes is probably not enough to prevent adolescents from smoking.
Using the Theory of Planned Behavior to Encourage Water Conservation among Extension Clients

Extension professionals can play a role in addressing water scarcity issues by helping home landscape irrigation users to conserve water. This study used survey research to examine the relationship between several variables, including attitudes, subjective norms, perceived behavioral control, personal norms, demographic factors, and past behaviors, on intention to use good irrigation practices among Florida home landscape irrigation users (N = 1,063). Following subsequent hierarchical linear regression models, the final model explained 39% of the variance in intentions to engage in good landscape irrigation practices. Subjective norms had a strong influence on intention to engage in landscape water conservation, and past behaviors and personal norms improved the prediction. Extension professionals should incorporate subjective norms into water conservation programs by emphasizing somewhat invisible conservation behaviors to improve perceptions of peers’ practices. When personal norms are strong, the subjective norms are slightly less important. Residents who feel a personal obligation to conserve water may be more open to information related to water conservation, and they may be more likely to act, even in the absence of social support. Finally, Extension professionals should consider the audience’s past behaviors to design programs that are compatible with actions that Extension clients are likely to take.
Corneal stromal changes induced by myopic LASIK.

UNLABELLED PURPOSE. Despite the rapidly growing popularity of laser in situ keratomileusis (LASIK) in correction of myopia, the tissue responses have not been thoroughly investigated. The aim was to characterize morphologic changes induced by myopic LASIK in human corneal stroma. METHODS Sixty-two myopic eyes were examined once at 3 days to 2 years after LASIK using in vivo confocal microscopy for measurement of flap thickness, keratocyte response zones, and objective grading of haze. RESULTS Confocal microscopy revealed corneal flap interface particles in 100% of eyes and microfolds at the Bowman's layer in 96.8%. The flaps were thinner (112 +/- 25 microm) than intended (160 microm). The keratocyte activation in the stromal bed was greatest on the third postoperative day. Patients with increased interface reflectivity due to abnormal extracellular matrix or activated keratocytes at > or = 1 month (n = 9) had significantly thinner flaps than patients with normal interface reflectivity (n = 18; 114 +/- 12 versus 132 +/- 22 microm, P = 0.027). After 6 months the mean density of the most anterior layer of flap keratocytes was decreased. CONCLUSIONS Keratocyte activation induced by LASIK was of short duration compared with that reported after photorefractive keratectomy. The flaps were thinner than expected, and microfolds and interface particles were common complications. The new findings such as increased interface reflectivity associated with thin flaps and the apparent loss of keratocytes in the most anterior flap 6 months to 2 years after surgery may have important clinical relevance.
The Parental Gender Earnings Gap in the United States

This paper examines the parental gender earnings gap, the within-couple differences in earnings over time, before and after the birth of a child. The presence and timing of children are important components of the gender wage gap, but there is selection in both decisions. We estimate the earnings gap between male and female spouses over time, which allows us to control for this timing choice as well as other shared external earnings shifters, such as the local labor market. We use Social Security Administration Detail Earnings Records (SSA-DER) data linked to the Survey of Income and Program Participation (SIPP) to examine a panel of earnings from 1978 to 2011 for the individuals in the SIPP sample. Our main results show that the spousal earnings gap doubles between two years before the birth of the first child and the year after that child is born. After the child's first year of life the gap continues to grow for the next five years, but at a much slower rate, then tapers off and even begins to fall once the child reaches school-age.
Evaluation of the protective effect of oestradiol against toxicity induced by 6‐hydroxydopamine and 1‐methyl‐4‐phenylpyridinium ion (MPP+) towards dopaminergic mesencephalic neurones in primary culture

Recent findings suggest that gonadal steroid hormones are neuroprotective and may provide clinical benefits in delaying the development of Parkinson's disease. In this report we investigated the ability of oestradiol to protect mesencephalic dopaminergic neurones cultured in serum‐free or serum‐supplemented medium from toxicity induced by 6‐hydroxydopamine or 1‐methyl‐4‐phenylpyridinium ion (MPP+). The efficiency of both toxins and oestradiol was evaluated by tyrosine hydroxylase (TH) immunocytochemistry, [3H]dopamine ([3H]DA) uptake, length of dopaminergic processes and␣lactate dehydrogenase (LDH) release measurement. In␣cultures grown in serum‐supplemented medium, a 2‐h pre‐treatment with high concentrations (10–100 µm) of 17β‐oestradiol or 17α‐oestradiol, the stereoisomer with weak oestrogenic activity, protected both dopaminergic and non‐dopaminergic neurones from toxicity induced by 6‐hydroxydopamine (6‐OHDA; 40 or 100 µm) and by the high MPP+ concentrations (50 µm) necessary to obtain significant neuronal death under those culture conditions. At these concentrations, MPP+ was no longer selective for dopaminergic neurones but affected all cells present in the culture. In contrast, the hormonal treatments did not protect against selective degeneration of dopaminergic neurones induced by lower MPP+ concentrations (below 10 µm), related to inhibition of complex I of respiratory chain. In cultures grown in serum‐free medium, oestradiol concentrations higher than 1 µm induced neuronal degeneration and no protection against 6‐OHDA or MPP+ toxicity was observed at lower concentrations of the steroid. The neuroprotective effects of 17α‐ or 17β‐oestradiol evidenced in this model might be due to the antioxidant properties of these compounds. However, other non‐genomic effects of the steroids cannot be excluded.
EMIGRATION FROM EASTERN EUROPE WITH A FOCUS ON BRAIN DRAIN

The objective of this paper is to offer a better understanding of the situation on emigration, with a focus on the emigration of the university-educated individuals, from 6 different Eastern European countries, namely Bulgaria, the Czech Republic, Hungary, Poland, Romania and the Slovak Republic. The period studied is 1980-2010, with a greater emphasis on the last decade, as the emigration from these countries increased sharply especially after their adhesion to the European Union. A regression analysis was performed, whose purpose is to determine the main factors that influence the brain drain. Data on tertiary educated emigration rates, unemployment rates of the tertiary educated, GDP per capita, wages adjusted for inflation, consumer price indexes and education expenditures were collected for the period 1980-2010 (5 year intervals) and in some cases 2011 as well, for all 6 Eastern European countries. The results showed that Romania and Poland are the most affected by the brain drain. The factors that were significant in shaping emigration were the wages (influenced by GDP) in these countries, as well as the education expenditure (influenced by the inflation).