Miracle-dz/UniFAR-FTUs huggingface dataset:
Congenital macrothrombocytopenia associated with a combination of functional polymorphisms in the TUBB1 gene

Summary Congenital thrombocytopenia in childhood and adolescence requires an extensive diagnostic workup to find the underlying reason. We report on a 13-year-old female patient who was incidentally found to have moderate thrombocytopenia which was also diagnosed in her father and brother. Within the microscopic evaluation of a peripheral blood smear macrothrombocytes were found. Immunofluorescence microscopy of the patient’s platelets detected the lack of D1-tubulin. Analysis of the TUBB1 gene revealed three known missense variants in heterozygous state which in combination might explain the D1-tubulin defect.
Stably transmitted triple-promoter retroviral vectors and their use in transformation of primary mammalian cells

Retroviral vectors were constructed which coexpressed three inserted genes from independent transcriptional promoters in singly infected cells. Several such triple-promoter vectors were constructed with various combinations of oncogenes and selectable drug resistance genes. All expressed three mRNAs of the expected size in infected cells. One vector expressing the v-Ha-ras, v-myc, and neo genes was characterized in detail. This retrovirus did not undergo rearrangement during the process of infection, as judged by Southern analysis, and infection of primary rat embryo fibroblasts demonstrated that ras-myc-cotransformed cells could be selected in G418. This demonstration that retroviral vectors can be used to express three cistrons independently increases their value as gene transfer vehicles, particularly for studies involving oncogene cooperation in primary cells.
Comprehensive Guide to Interpersonal Psychotherapy

Eventually, you will definitely discover a new experience and execution by spending more cash. still when? attain you assume that you require to get those all needs later having significantly cash? Why dont you try to acquire something basic in the beginning? Thats something that will guide you to comprehend even more on the globe, experience, some places, taking into account history, amusement, and a lot more?
Direct electrochemistry of the hydroxylase of soluble methane monooxygenase from Methylococcus capsulatus (Bath).

The redox properties of the hydroxylase component of soluble methane monooxygenase from Methylococcus capsulatus (Bath) have been thoroughly investigated. Previous studies used redox indicator titrations and spectroscopic methods for the determination of the concentrations of reduced species. Herein we report, for the first time, direct electrochemistry (i.e. without the use of mediators) of the diiron centers of the hydroxylase from M. capsulatus (Bath) at a modified gold electrode giving rise to two waves at 4(+/- 10) mV and -386(+/- 14) mV versus saturated calomel electrode (SCE). In addition, the effects of proteins B and B' on the redox reactions were determined. The redox potentials of the complex with protein B are -25(+/- 14) mV and -433(+/- 8) mV versus SCE whereas protein B' had no effect though it did alter the effect of protein B on the redox potentials.
Shareholder Litigation and Management Earnings Forecasts

In this paper, we establish a causal relation between litigation risk and management earnings forecasts, exploiting the exogenous reduction in litigation threat generated by the 1999 ruling of the Ninth Circuit Court of Appeals. Following the ruling, we find that the treated firms in the Ninth Circuit states, tend to make fewer management earnings forecasts, and are particularly reluctant to convey negative news. They also deliver forecasts in a less timely fashion and with lower precision and accuracy. Taken together, these results are consistent with the “litigation deterrence” motive of making earnings forecasts. Finally, we investigate the interaction of the litigation environment and firms’ capital market needs in jointly shaping earnings forecast practices. We find that the reduction of negative news forecasts is more pronounced for firms with large financial constraints and/or a greater need for external funds.
Distinguishing the Components of Household Financial Wealth: the Impact of Liabilities on Assets in Euro Area Countries

This paper studies the interdependence of households? financial assets and liabilities in European countries. Most studies treat household liabilities as negative assets and relate households? decisions to their net wealth. However, the components of net wealth, i.e. financial liabilities, financial assets and real assets are very heterogeneous across households. The assumption of similar consumption behaviour among households with the same net wealth but different wealth components is implausible. This paper raises another question, namely whether households consider their indebtedness when they make decisions about their financial assets. The implication of household indebtedness for household behaviour is an important topic as household debt volumes have increased markedly in developed countries over the last three decades. There is a long list of research about household borrowing and the financial vulnerability of indebted households, but there has been less discussion about whether and how indebtedness affects households? behaviour beyond their borrowing decisions. The indebtedness has implications for households? consumption behaviour as well as for their choices regarding financial assets. There is a lack of empirical evidence on the effect of indebtedness on households? financial asset holdings in the middle of a recession. This paper sheds more light on the relationship between liabilities and financial asset holdings. The paper uses data from the recently introduced Household Finance and Consumption Sur-vey. The paper uses the first wave of the HFCS, which was implemented in 2009-2010 in 13 euro area countries. A system of equations for financial liabilities and financial assets is esti-mated while allowing for endogeneity of the two wealth components. Furthermore, selection bias issues are addressed by estimating the control equation for the selection model of debt ownership. The results suggest that households? liabilities impact households? financial assets negatively while no significant effect was found from financial assets to liabilities. The nega-tive impact of liabilities on financial assets remains after controls for the debt service burden and for saving to pay back debt are included. The results are confirmed by a large number of robustness tests. The findings provide empirical evidence for the theoretical assumption that credit markets reduce the holdings of financial assets of households. The results posit that increasing volumes of household debt are followed by lower incentives to keep financial assets. It is particularly important to understand how households? liabilities affect their other financial decisions as the penetration of debt and the volumes of debt have increased.
Safety of recombinant human deoxyribonuclease as a rescue treatment for persistent atelectasis in newborns

BACKGROUND AND OBJECTIVE Pulmonary problems are vitally important in newborns. Increased intense and mucoid secretions may lead to atelectasis, pulmonary infections, respiratory distress, prolonged mechanical ventilation or even death. The aim of this study was to evaluate the safety of recombinant human deoxyribonuclease (rhDNase) in the management of persistent atelectasis in term and preterm newborns, unresponsive to the conventional treatment. DESIGN AND SETTING Prospective study of patients admitted to a general community setting of a neonatal intensive care unit between December 2007 and December 2009. PATIENTS AND METHODS The study included 22 patients (12 premature and 10 term) who were admitted to the neonatal intensive care unit because of respiratory distress and developed atelectasis, and were unresponsive to conventional treatment. Nebulized rhDNase was administered to all patients at a dose of 1 mg/m2 twice daily for 3 days. In patients who did not respond to 3 days of treatment, endotracheal rhDNase was administered at a dose of 1 mg/m2. We assessed the clinical (respiratory rate and oxygen requirement) and radiologic responses (chest radiographic score), recurrence of atelectasis, the need for a repetitive treatment, and mortality rate. RESULTS A clinical and radiologic improvement of atelectasis was observed in 18 of 22 patients following 3 days of nebulized rhDNase treatment. Atelectasis relapsed in 4 patients. Following the administration of combined endotracheal and nebulized rhDNase treatment, an improvement of atelectasis was noted in all four recurrent cases. No adverse events were observed in patients because of the rhDNase treatment. CONCLUSIONS rhDNase treatment is a safe option and may be used as an effective method for the management of persistent atelectasis in newborns, which is resistant to other conventional treatment methods.
Plant‐derived pharmaceuticals for the developing world

Plant‐produced vaccines and therapeutic agents offer enormous potential for providing relief to developing countries by reducing the incidence of infant mortality caused by infectious diseases. Vaccines derived from plants have been demonstrated to effectively elicit an immune response. Biopharmaceuticals produced in plants are inexpensive to produce, require fewer expensive purification steps, and can be stored at ambient temperatures for prolonged periods of time. As a result, plant‐produced biopharmaceuticals have the potential to be more accessible to the rural poor. This review describes current progress with respect to plant‐produced biopharmaceuticals, with a particular emphasis on those that target developing countries. Specific emphasis is given to recent research on the production of plant‐produced vaccines toward human immunodeficiency virus, malaria, tuberculosis, hepatitis B virus, Ebola virus, human papillomavirus, rabies virus and common diarrheal diseases. Production platforms used to express vaccines in plants, including nuclear and chloroplast transformation, and the use of viral expression vectors, are described in this review. The review concludes by outlining the next steps for plant‐produced vaccines to achieve their goal of providing safe, efficacious and inexpensive vaccines to the developing world.
Primary chemotherapy and delayed surgery for nonmetastatic osteosarcoma of the extremities. Results in 164 patients preoperatively treated with high doses of methotrexate followed by cisplatin and doxorubicin

Background. Neoadjuvant chemotherapy is the most accepted treatment for localized osteosarcoma. This has led to a great improvement in limb‐sparing surgery and in disease‐free survival. Patients with a good response to preoperative chemotherapy showed a higher disease‐free survival rate. Current studies examine the possibility of patients whose limbs could be rescued with a poor necrosis and a reduction of the side effects related to aggressive treatments.
THE JOB SATISFACTION -EMPLOYEE PERFORMANCE RELATIONSHIP: A THEORETICAL PERSPECTIVE Miss Vartikka Indermun Academic Lecturer at Management College of Southern Africa (MANCOSA) &

Organisations face strong pressures in competitive environments to be efficient and at the same time produce products of value. By ensuring that their workforce is optimal at all times most organisations can gain competitive advantage. Satisfied employees form a bond with the company and take pride in their organisational membership, they believe in the goals and values of the organisation. Therefore, these employees display high levels of performance and productivity. Dissatisfied employees display characteristics of low productivity, absenteeism, and turnover. These traits are highly costly for the organisation. Therefore, it is crucial that research is done to determine the relationship between job satisfaction and employee performance.
Effects of Strength Training on Running Economy in Highly Trained Runners: A Systematic Review With Meta-Analysis of Controlled Trials

Abstract Balsalobre-Fernández, C, Santos-Concejero, J, and Grivas, GV. Effects of strength training on running economy in highly trained runners: a systematic review with meta-analysis of controlled trials. J Strength Cond Res 30(8): 2361–2368, 2016—The purpose of this study was to perform a systematic review and meta-analysis of controlled trials to determine the effect of strength training programs on the running economy (RE) of high-level middle- and long-distance runners. Four electronic databases were searched in September 2015 (PubMed, SPORTDiscus, MEDLINE, and CINAHL) for original research articles. After analyzing 699 resultant original articles, studies were included if the following criteria were met: (a) participants were competitive middle- or long-distance runners; (b) participants had a V[Combining Dot Above]O2max >60 ml·kg−1·min−1; (c) studies were controlled trials published in peer-reviewed journals; (d) studies analyzed the effects of strength training programs with a duration greater than 4 weeks; and (e) RE was measured before and after the strength training intervention. Five studies met the inclusion criteria, resulting in a total sample size of 93 competitive, high-level middle- and long-distance runners. Four of the 5 included studies used low to moderate training intensities (40–70% one repetition maximum), and all of them used low to moderate training volume (2–4 resistance lower-body exercises plus up to 200 jumps and 5–10 short sprints) 2–3 times per week for 8–12 weeks. The meta-analyzed effect of strength training programs on RE in high-level middle- and long-distance runners showed a large, beneficial effect (standardized mean difference [95% confidence interval] = −1.42 [−2.23 to −0.60]). In conclusion, a strength training program including low to high intensity resistance exercises and plyometric exercises performed 2–3 times per week for 8–12 weeks is an appropriate strategy to improve RE in highly trained middle- and long-distance runners.
Microclustering of TEL‐AML1 translocation breakpoints in childhood acute lymphoblastic leukemia

TEL‐AML1 fusions are the most common chromosome translocations in childhood leukemia and often, if not always, occur in utero. We previously reported the genomic sequencing of nine TEL‐AML1 translocations and showed unique structural features of a breakpoint cluster region in TEL intron 5. We now report data on sequencing and mapping of TEL‐AML1 from an additional 11 patients and, using Monte Carlo statistical methods, have analyzed the intronic distribution of the 24 TEL‐AML1 fusion junctions sequenced to date. Compared to a null hypothesis of random breakpoint allocation within TEL intron 5 and AML1 introns 1 and 2, significant microclustering was evident on both TEL and AML1. In contrast to previous reports, the two strongest microclusters on TEL were 3′ to an unstable repeat region. AML1 demonstrated four highly significant microclusters, two of which were proximal to exons. We note the necessity of sequencing multiple breakpoints before the description of putative microcluster regions. TEL‐AML1 breakpoints may be distributed into microclusters because of specific DNA sequence or chromatin features in susceptible cells. We also report on additional features of breakpoints, including a complex t(12;3;21) in one patient and an inverted sequence in another. © 2000 Wiley‐Liss, Inc.
Correlation of Ultrasonography and Bacteriology of the Endocervlx and Posterior Cul‐de-Sac of Patients with Severe Pelvic Inflammatory Disease

We evaluated 30 patients admitted with a diagnosis of pelvic inflammatory disease (PID) by culdocentesis and ultrasonography to determine the severity of disease in relation to endocervical and culdocentesis bacteriology. Specimens from the endocervix were cultured for Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, and Ureaplasma urealyticum. In addition, culdocentesis fluid was cultured for aerobes and anaerobes. Patients with N. gonorrhoeae or C. trachomatis had significantly milder disease (P less than 0.05) compared with patients whose specimens were negative for those organisms. Furthermore, those patients with ultrasound confirmation of a tubo-ovarian complex had significantly (P less than 0.0001) greater recovery of aerobes and anaerobes from culdocentesis fluid than did patients with milder disease.
The Effect of Communication Satisfaction on Organizational Commitment

Organizational commitment is to make an effort with everyone from the personnel at the bottom level to the top management in order for the organization to believe in its goals and objectives and to achieve these. In order to make this effort, there must be a strong communicational connection within the organization. Communication satisfaction among the employees within the organization may also affect the organizational commitment. From this point of view, the organizational commitment and internal communication satisfaction of the employees, one of the most important values of the organizations, were studied and the effect of commination satisfaction on organizational commitment was revealed having the link between these two concepts examined. For this study, the employees of Garanti Bank, one of the most influential banks in Turkey’s banking sector were surveyed and this mentioned effect was examined using communication satisfaction and commitment surveys.
Let Me In! The Predictive Validity of Graduate Management Admissions Test and Other Variables in the Admission System of Masters of Business Administration Programmes

Data were collected from a Masters of Business Administration (MBA) program in a public university in the southern part of Texas. Analysis of variance results indicate that there are gender as well as ethnicity effects. Similarly, regression results indicate that only ethnicity and gender of the subjects contributed significantly in predicting the students’ graduate grade point average. However, different predictors account for the variance depending on the ethnicity/gender. Women scored lower graduate management admissions tests, but they surpassed their counterparts in academic performance during the MBA programme, raising questions as to the predictive validity of such exam. Finally, a change in the graduate admission system calls for consideration of soft issues when engaging in processes reengineering.
Evidence that a transcortical pathway contributes to stretch reflexes in the tibialis anterior muscle in man

1 In human subjects, stretch applied to ankle dorsiflexors elicited three bursts of reflex activity in the tibialis anterior (TA) muscle (labelled M1, M2 and M3) at mean onset latencies of 44, 69 and 95 ms, respectively. The possibility that the later of these reflex bursts is mediated by a transcortical pathway was investigated. 2 The stretch evoked a cerebral potential recorded from the somatosensory cortex at a mean onset latency of 47 ms in nine subjects. In the same subjects a compound motor‐evoked potential (MEP) in the TA muscle, evoked by magnetic stimulation of the motor cortex, had a mean onset latency of 32 ms. The M1 and the M2 reflexes thus had too short a latency to be caused by a transcortical pathway (minimum latency, 79 ms (47 + 32)), whereas the later part of the M2 and all of the M3 reflex had a sufficiently long latency. 3 When the transcranial magnetic stimulation was timed so that the MEP arrived in the TA muscle at the same time as the M1 or M2 reflexes, no extra increase in the potential was observed. However, when the MEP arrived at the same time as the M3 reflex a significant (P < 0.01) extra‐facilitation was observed in all twelve subjects investigated. 4 Peaks evoked by transcranial magnetic stimulation in the post‐stimulus time histogram of the discharge probability of single TA motor units (n= 28) were strongly facilitated when they occurred at the same time as the M3 response. This was not the case for the first peaks evoked by electrical transcranial stimulation in any of nine units investigated. 5 We suggest that these findings are explained by an increased cortical excitability following TA stretch and that this supports the hypothesis that the M3 response in the TA muscle is ‐ at least partly ‐ mediated by a transcortical reflex.
Branched structures in the intracellular DNA of herpes simplex virus type 1

Herpes simplex virus type 1 (HSV-1) replication produces large intracellular DNA molecules that appear to be in a head-to-tail concatemeric arrangement. We have previously suggested (A. Severini, A.R. Morgan, D.R. Tovell, and D.L.J. Tyrrell, Virology 200:428-435, 1994) that these DNA species may have a complex branched structure. We now provide direct evidence for the presence of branches in the high-molecular-weight DNA produced during HSV-1 replication. On neutral agarose two-dimensional gel electrophoresis, a technique that allows separation of branched restriction fragments from linear fragments, intracellular HSV-1 DNA produces arches characteristic of Y junctions (such as replication forks) and X junctions (such as merging replication forks or recombination intermediates). Branched structures were resolved by T7 phage endonuclease I (gene 3 endonuclease), an enzyme that specifically linearizes Y and X structures. Resolution was detected by the disappearance of the arches on two-dimensional gel electrophoresis. Branched structures were also visualized by electron microscopy. Molecules with a single Y junction were observed, as well as large tangles containing two or more consecutive Y junctions. We had previously shown that a restriction enzyme which cuts the HSV-1 genome once does not resolve the large structure of HSV-1 intracellular DNA on pulsed-field gel electrophoresis. We have confirmed that result by using sucrose gradient sedimentation, in which both undigested and digested replicative intermediates sediment to the bottom of the gradient. Taken together, our experiments show that the intracellular HSV-1 DNA is held together in a large complex by frequent branches that create a network of replicating molecules. The fact that most of these branches are Y structures suggests that the network is held together by frequent replication forks and that it resembles the replicative intermediates of bacteriophage T4. Our findings add complexity to the simple model of rolling-circle DNA replication, and they pose interesting questions as to how the network is formed and how it is resolved for packaging into progeny virions.
Iterative Decision Directed Channel Estimation for BICM-Based MIMO-OFDM Systems

Iterative Decision Directed Channel Estimation (DDCE) scheme is proposed in this paper for bit interleaved coded modulation (BICM)-based MIMO-OFDM systems. The proposed scheme employs Variable Step Size Normalized Least Mean Square (VSSNLMS) algorithm to implement its temporary Channel Transfer Function (CTF) Estimator and Adaptive Channel Impulse Response (CIR) predictor modules, while Fast Data Projection Method (FDPM) algorithm is employed to track the time domain CIR of the MIMO channel. The proposed iterative DDCE operates in an iterative mode in conjunction with MIMO demappers and the Turbo decoder at receiver of the MIMO-OFDM system. The achievable performances of the proposed scheme in terms of both mean square error (MSE) and bit error rate (BER) are presented. Comparative performances between the DDCE scheme employing FDPM algorithm and another one employing deflated Projection Approximation Subspace Tracking (PASTd) algorithm are also given. The simulations results presented indicate better performances of the proposed scheme.