Miracle-dz/UniFAR-FTUs huggingface dataset:
Acquired Bacitracin Resistance in Enterococcus faecalis Is Mediated by an ABC Transporter and a Novel Regulatory Protein, BcrR

ABSTRACT Bacitracin resistance (bacitracin MIC, ≥256 μg ml−1) has been reported in Enterococcus faecalis, and in the present study we report on the genetic basis for this resistance. Mutagenesis was carried out with transposon Tn917 to select for E. faecalis mutants with decreased resistance to bacitracin. Two bacitracin-sensitive mutants (MICs, 32 μg ml−1) were obtained and Tn917 insertions were mapped to genes designated bcrA and bcrB. The amino acid sequences of BcrA (ATP-binding domain) and BrcB (membrane-spanning domain) are predicted to constitute a homodimeric ATP-binding cassette (ABC) transporter, the function of which is essential for bacitracin resistance in E. faecalis. The bcrA and bcrB genes were organized in an operon with a third gene, bcrD, that had homology to undecaprenol kinases. Northern analysis demonstrated that bcrA, bcrB, and bcrD were transcribed as a polycistronic message that was induced by increasing concentrations of bacitracin but not by other cell wall-active antimicrobials (e.g., vancomycin). Upstream of the bcrABD operon was a putative regulatory gene, bcrR. The bcrR gene was expressed constitutively, and deletion of bcrR resulted in a bacitracin-sensitive phenotype. No bcrABD expression was observed in a bcrR mutant, suggesting that BcrR is an activator of genes essential for bacitracin resistance (i.e., bcrABD). The bacitracin resistance genes were found to be located on a plasmid that transferred at a high frequency to E. faecalis strain JH2-2. This report represents the first description of genes that are essential for acquired bacitracin resistance in E. faecalis.
Diversity of carbapenemases in clinical isolates of Enterobacteriaceae in Croatia--the results of a multicentre study.

Since the first carbapenem-resistant Klebsiella pneumoniae strain was isolated in 2008, Enterobacteriaceae with reduced susceptibility to one or more carbapenems have emerged sporadically in different geographical regions in Croatia. These observations gave rise to a multicenter study on carbapenem resistance in Enterobacteriaceae from Croatia. Fifty-seven carbapenem-non-susceptible strains of Enterobacteriaceae were collected during 2011-2012 from four large hospital centres in Croatia. Overall, 36 strains produced VIM-1 β-lactamase, three produced NDM-1, and one produced KPC-2. A high degree of clonal relatedness was observed in Enterobacter cloacae and Citrobacter freundii strains, in contrast to K. pneumoniae strains. BlaVIM genes were located within class1 integron which contained genes encoding resistance to aminoglycosides (aacA4 ). The study found strong association between blaVIM and qnrB6 and between blaNDM and qnrA6 genes.
Hiding Images in Plain Sight: Deep Steganography

Steganography is the practice of concealing a secret message within another, ordinary, message. Commonly, steganography is used to unobtrusively hide a small message within the noisy regions of a larger image. In this study, we attempt to place a full size color image within another image of the same size. Deep neural networks are simultaneously trained to create the hiding and revealing processes and are designed to specifically work as a pair. The system is trained on images drawn randomly from the ImageNet database, and works well on natural images from a wide variety of sources. Beyond demonstrating the successful application of deep learning to hiding images, we carefully examine how the result is achieved and explore extensions. Unlike many popular steganographic methods that encode the secret message within the least significant bits of the carrier image, our approach compresses and distributes the secret image's representation across all of the available bits.
Multiple comparisons with two controls for ordered categorical responses

ABSTRACT In clinical studies, ordered categorical responses are common. To compare the efficacy of several treatments with a control for ordinal responses, the normal latent variable model has recently been proposed. This approach conceptualizes the responses as manifestations of an underlying continuous normal variable. In this article, we extend this idea to develop the multiple comparison method for use when there are two controls in the clinical trial. The proposed method is constructed such that the familywise type I error rate is controlled at a prespecified level. In addition, for a given level of test power, the procedure to evaluate the required sample size is provided. The proposed testing procedure is also illustrated by an example from a clinical study.
Effects of off-pump coronary surgery on the mechanics of the respiratory system, lung, and chest wall: Comparison with extracorporeal circulation

Objective To compare the effects of cardiac surgery with and without extracorporeal circulation on the mechanics of the respiratory system, lung, and chest wall. We also determined the time course of those effects. Design Prospective, controlled study. Setting An eight-bed, cardiac-surgical intensive care unit at a university hospital. Patients Two groups of patients scheduled for elective coronary bypass surgery were studied: ten patients with extracorporeal circulation and 13 patients without extracorporeal circulation. Interventions Measurement of esophageal pressure after insertion of an esophageal balloon catheter to separate respiratory system mechanics into lung and chest wall components. Measurements were performed preoperatively after induction of anesthesia (control), immediately postoperatively at arrival in the intensive care unit (time 1), and after 3 hrs (time 2). In 12 of the 23 patients, measurements were also performed 6 hrs postoperatively (time 3). Measurements and Main Results No significant differences concerning demographics or surgical procedure were noticed between the two groups. Respiratory system, chest wall, and lung mechanics were obtained using the technique of rapid airway occlusion during constant-flow inflation. In both the group with and without extracorporeal circulation there was a significant increase in static and dynamic elastance of the respiratory system and lung at times 1 and 2, which tended to decrease again at time 3; chest wall elastance significantly increased at times 2 and 3 in the group without extracorporeal circulation, whereas the increase in chest wall elastance in the group with extracorporeal circulation occurred earlier (also at time 1). Additional resistance of the respiratory system and lung remained unchanged; chest wall resistance, however, significantly increased in both groups. Work of breathing significantly increased in both groups at times 1 and 2. There was a significant reduction in the Pao2/Fio2 ratio in both groups at times 2 and 3. No significant differences between the groups at any moment were noticed. Conclusions Coronary bypass surgery with and without extracorporeal circulation results in dramatic impairment of respiratory system mechanics. Based on respiratory system mechanics, early extubation after coronary artery bypass grafting should be performed with caution, no matter whether the off-pump or cardiopulmonary bypass technique is used.
Prediction of sulfamethoxazole-trimethoprim synergistic action against members of the family Enterobacteriaceae with a two-plate agar dilution breakpoint MIC system

Synergy between sulfamethoxazole (SMZ) and trimethoprim (TMP) was predicted by a two-plate agar dilution breakpoint MIC system. Comparison of the results of this new system with those of the disk diffusion system (P.M. Waterworth, Postgrad. Med. J. Suppl. 45:21-27, 1969) after tests with 1,518 Enterobacteriaceae isolates showed an overall correlation of 99.8%, a sensitivity of 99.7%, and a specificity of 100%. The method involves spot inoculation of 10(3) organisms onto each of two plates, one containing 160 micrograms of SMZ per ml and the other 8 micrograms of TMP per ml (in Oxoid IsoSensitest medium with 3% agar supplemented with 7% saponin-lysed horse blood), and then incubation overnight at 37 degrees C in air. All but three organisms for which SMZ-TMP was found to be synergistic by disk testing were inhibited on both plates. Three isolates of Proteus mirabilis, which failed to correlate with disk testing by this new system, all showed SMZ MICs of 1,000 micrograms/ml. The SMZ-TMP combination was falsely predicted to be nonsynergistic against these three organisms. There were no false synergy predictions by the breakpoint MIC system. Laboratories should report susceptibility to the SMZ-TMP combination only when there is synergy between the constituents. This simple, reliable agar dilution technique enables laboratories to accurately report synergy between SMZ and TMP.
Overview of small bowel angioectasias: clinical presentation and treatment options

ABSTRACT Introduction: Elderly patients with multiple co-morbidities are at an increased risk of developing small bowel angioectasias. Treating these lesions can be both challenging and costly with patients requiring extensive investigations and recurrent admissions for iron infusions and blood transfusions as well as invasive procedures. This review presents treatment options and describes in detail drugs that should be considered whilst taking into account their effectiveness and their safety profile. Areas covered: A PubMed search was carried out using the following keywords: small bowel angiodysplasias, small bowel angioectasias, small bowel bleeding and obscure gastrointestinal bleeding to assess existing evidence. The pathophysiology and risk factors are covered in this review together with appropriate methods of investigation and management. Treatment options discussed are endoscopic measures, surgical options and pharmacotherapy. The role of serum biomarkers is also discussed. Expert commentary: Future work should be directed at alternative drugs with a good safety profile that target biomarkers. Novel pharmacotherapy directed at biomarkers could potentially provide a non-invasive treatment option for angioectasias particularly in the elderly where management can be challenging.
Ionotropic glutamate receptor binding in the posterior cingulate cortex in schizophrenia patients

Using quantitative autoradiography, the present study examined ionotropic glutamatergic receptor binding sites using [3H]dizocilpine, [3H]&agr;-amino-3-hydroxy-5-methylisoxazole-4-propionate, and [3H]kainate in the posterior cingulate cortex of schizophrenia patients and matched controls. We found a significant increase in [3H]dizocilpine binding in the superficial layers (41%, p<0.001) and deep layers (30%, p=0.004) of the posterior cingulate cortex in the schizophrenia group compared with controls. No significant differences were observed in [3H]&agr;-amino-3-hydroxy-5-methylisoxazole-4-propionate and [3H]kainate binding. In summary, the present study has for the first time demonstrated that the glutamatergic system is affected in the posterior cingulate cortex in schizophrenia patients. The fact that only the N-methyl-D-aspartate receptor densities are significantly altered suggests that this is unlikely to be caused by a simple decrease in glutamatergic transmission.
Specific Delay in the Degradation of Mitochondrial ATP Synthase Subunit c in Late Infantile Neuronal Ceroid Lipofuscinosis Is Derived from Cellular Proteolytic Dysfunction Rather than Structural Alteration of Subunit c

Abstract: Previously we indicated that a specific delay in subunit c degradation causes the accumulation of mitochondrial ATP synthase subunit c in lysosomes from the cells of patients with the late infantile form of neuronal ceroid lipofuscinosis (NCL). To explore the mechanism of lysosomal storage of subunit c in patient cells, we investigated the mechanism of the lysosomal accumulation of subunit c both in cultured normal fibroblasts and in in vitro cell‐free incubation experiments. Addition of pepstatin to normal fibroblasts causes the marked lysosomal accumulation of subunit c and less accumulation of Mn2+‐superoxide dismutase (SOD). In contrast, E‐64‐d stimulates greater lysosomal storage of Mn2+‐SOD than of subunit c. Incubation of mitochondrial‐lysosomal fractions from control and diseased cells at acidic pH leads to a much more rapid degradation of subunit c in control cells than in diseased cells, whereas other mitochondrial proteins, including Mn2+‐SOD, β subunit of ATP synthase, and subunit IV of cytochrome oxidase, are degraded at similar rates in both control and patient cells. The proteolysis of subunit c in normal cell extracts is inhibited markedly by pepstatin and weakly by E‐64‐c, as in the cultured cell experiments. However, there are no differences in the lysosomal protease levels, including the levels of the pepstatin‐sensitive aspartic protease cathepsin D between control and patient cells. The stable subunit c in mitochondrial‐lysosomal fractions from patient cells is degraded on incubation with mitochondrial‐lysosomal fractions from control cells. Exchange experiments using radiolabeled substrates and nonlabeled proteolytic sources from control and patient cells showed that proteolytic dysfunction, rather than structural alterations such as the posttranslational modification of subunit c, is responsible for the specific delay in the degradation of subunit c in the late infantile form of NCL.
Dependency of standardized uptake values of fluorine-18 fluorodeoxyglucose on body size: comparison of body surface area correction and lean body mass correction.

In an attempt to minimize dependency of conventional standardized uptake values (SUVs) of F-18-fluorodeoxyglucose (FDG) normalized for the total body weight (TBW) on patient's body weight, the uses of SUV corrected for the lean body mass (LBM) and that corrected for the body surface area (BSA) have been proposed as alternatives. We compared the dependency of SUVLBM and SUVBSA on the overall body size. FDG-PET images were acquired on 44 patients. SUVLBM and SUVBSA for the liver were determined as previously described. Following normalization of the scales of the values obtained from these two approaches, the mean +/- S.D. was 2.86 +/- 0.52 (SUVLBM) and 2.86 +/- 0.50 (SUVBSA). A linear regression analysis was performed for correlating the liver SUVLBM and SUVBSA, with each of TBW, LBM, BSA and height. The dependency of the two values on TBW was similar. SUVLBM showed a moderate dependency on height (r = 0.56, P = 0.00007), LBM (r = 0.55, P = 0.0001) and BSA (r = 0.51, P = 0.0004), whereas SUVBSA showed no dependency on LBM (r = 0.18, P = 0.24) or height (r = 0.20, P = 0.19), and a minimal dependency on BSA (r = 0.38, P = 0.01). In conclusion, SUVBSA appears to be less dependent on overall body size, particularly height and lean body mass, than SUVLBM.
Magnitude of hepatitis C virus infection in India: Prevalence in healthy blood donors, acute and chronic liver diseases

An enzyme immunoassay (EIA) was developed in‐house for the detection of anti‐hepatitis C virus (HCV) antibody against the prevailing genotypes in India. The specific reactivity of the test was compared with commercial second and third‐generation EIAs and reverse transcription nested polymerase chain reaction (RT‐nested PCR). Fifteen thousand nine hundred twenty‐two healthy blood donors at the All India Institute of Medical Sciences (AIIMS), New Delhi, India, were screened for anti‐HCV antibody. Two hundred ninety‐five (1.85%) of these donors were positive. The screening was also used to determine how many patients with acute hepatitis and chronic liver diseases were positive for anti‐HCV antibody. Five hundred sixty‐four chronic liver disease patients were screened for anti‐HCV antibody and 78 (13.83%) were found positive. Two hundred forty‐seven sporadic acute viral hepatitis patients were screened for viral infection markers. Hepatitis B and E viruses (HBV and HEV) were the major etiologic agents. HCV was associated with 9% of the acute cases. Anti‐HCV core IgM with HCV RNA detection were found to be helpful for the diagnosis of acute HCV infection. J. Med. Virol. 51:167–174, 1997. © 1997 Wiley‐Liss, Inc.
Landscape and Local Correlates of Bee Abundance and Species Richness in Urban Gardens

Abstract Urban gardens may preserve biodiversity as urban population densities increase, but this strongly depends on the characteristics of the gardens and the landscapes in which they are embedded. We investigated whether local and landscape characteristics are important correlates of bee (Hymenoptera: Apiformes) abundance and species richness in urban community gardens. We worked in 19 gardens in the California central coast and sampled bees with aerial nets and pan traps. We measured local characteristics (i.e., vegetation and ground cover) and used the USGS National Land Cover Database to classify the landscape surrounding our garden study sites at 2 km scales. We classified bees according to nesting type (i.e., cavity, ground) and body size and determined which local and landscape characteristics correlate with bee community characteristics. We found 55 bee species. One landscape and several local factors correlated with differences in bee abundance and richness for all bees, cavity-nesting bees, ground-nesting bees, and different sized bees. Generally, bees were more abundant and species rich in bigger gardens, in gardens with higher floral abundance, less mulch cover, more bare ground, and with more grass. Medium bees were less abundant in sites surrounded by more medium intensity developed land within 2 km. The fact that local factors were generally more important drivers of bee abundance and richness indicates a potential for gardeners to promote bee conservation by altering local management practices. In particular, increasing floral abundance, decreasing use of mulch, and providing bare ground may promote bees in urban gardens.
Investigating the 10 kA HTS Module of the ITER Current Leads

The International Thermonuclear Experimental Reactor (ITER) has 60 high-temperature superconducting (HTS) current leads (CL) which have a nominal current of 68, 55 and 10 kA (depending on the type of magnet they have to supply) and provide a total current of 2.7 MA to the magnet system. The HTS module is a key part of CLs. This paper describes the simulation of the critical current in a multi-layer tape model as well as the special soldering technique developed for the HTS stack. In order to validate this technique, a 10 kA pre-prototype HTS module was made and tested in a setup that reproduces ITER conditions at ASIPP. The paper also briefly describes the overheating time test setup and the results.
Redistributive Capital Taxation Revisited

This paper uses a rich quantitative model with endogenous skill acquisition to show that capital-skill complementarity provides a quantitatively significant rationale to tax capital for redistributive governments. The optimal capital income tax rate is 67 percent, while it is 61 percent in an identically calibrated model without capital-skill complementarity. The skill premium falls from 1.9 to 1.84 along the transition following the optimal reform in the capital-skill complementarity model, implying substantial indirect redistribution from skilled to unskilled workers. These results show that a redistributive government should take into account capital-skill complementarity when taxing capital. (JEL D31, H21, H23, H24, H25, J24, J31)
Noninvasive prediction of intracranial pressure curves using transcranial Doppler ultrasonography and blood pressure curves.

BACKGROUND AND PURPOSE Until now the assessment of intracranial pressure (ICP) required invasive methods. The objective of this study was to introduce an approach to a noninvasive assessment of continuous ICP curves. METHODS The intracranial compartment was considered a "black box" system with an input signal, the arterial blood pressure (ABP), and an output signal, the ICP. A so-called weight function described the relationship between ABP and ICP curves. Certain parameters, called transcranial Doppler (TCD) characteristics, were calculated from the cerebral blood flow velocity (FV) and the ABP curves and were used to estimate this weight function. From simultaneously sampled FV, ABP, and (invasively measured) ICP curves of a defined group of patients with severe head injuries, the TCD characteristics and the weight function were computed. Multiple regression analysis revealed a mathematical formula for calculating the weight function from TCD characteristics. This formula was used to generate the ICP simulation. FV, ABP, and ICP recordings from 11 patients (mean age, 46 +/- 14 years) with severe head injury were studied. In each patient, ICP was computed by a simulation procedure, generated from the data of the remaining 10 patients. The simulation period was 100 seconds. RESULTS Corresponding pressure trends with a mean absolute difference of 4.0 +/- 1.8 mm Hg between computed and measured ICP were observed. Shapes of pulse and respiratory ICP modulations were clearly predicted. CONCLUSIONS These results demonstrate that this method constitutes a promising step toward a noninvasive ICP prediction that may be clinically applicable under well-defined conditions.
Trust in Health Information Sources Differs between Young/Middle and Oldest Old

Purpose. Examine differences in trust of health information sources between the oldest old and young/middle old. Design. Cross-sectional survey using convenience sampling. Setting. Eleven retirement communities. Subjects. Older adults ≥ 65 years (N = 353). Measures. Self rated trust in health information sources. Analysis. Mann-Whitney U-test or Fisher exact test to compare trust between age groups; multinomial ordered logistic regression analyses to model trust in Internet information sources. Results. The overall survey response rate was 26.6%. Differences in trust were identified between oldest old (n = 108) and young/middle old (n = 245) for pharmacist (p < .05), Internet (p < .001), television (p < .05), radio (p < .001), and newspaper (p < .05) sources. In the oldest old, we found associations between levels of trust in Internet sources and frequency of Internet use (β = 4.13, p < .001). Conclusion. Understanding where differences in trust arise can inform the design of resources to support the information-seeking process. When planning widespread distribution of health information to these distinct groups, program developers need to consider these differences.