Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional gradients of the cerebellum

A central principle for understanding the cerebral cortex is that macroscale anatomy reflects a functional hierarchy from primary to transmodal processing. In contrast, the central axis of motor and nonmotor macroscale organization in the cerebellum remains unknown. Here we applied diffusion map embedding to resting-state data from the Human Connectome Project dataset (n=1003), and show for the first time that cerebellar functional regions follow a gradual organization which progresses from primary (motor) to transmodal (DMN, task-unfocused) regions. A secondary axis extends from task-unfocused to task-focused processing. Further, these two principal gradients reveal functional properties of the well-established cerebellar double motor representation, and its relationship with the recently described triple nonmotor representation. These interpretations are further supported by data-driven clustering and cerebello-cerebral functional connectivity analyses. Importantly, these descriptions remain observable at the individual subject level. These findings, from an exceptionally large and high-quality dataset, provide new and fundamental insights into the functional organization of the human cerebellum, unmask new testable hypotheses for future studies, and yield an unprecedented tool for the topographical, macroscale interpretation of cerebellar findings.
Grief and Avoidant Death Attitudes Combine to Predict the Fading Affect Bias

The fading affect bias (FAB) occurs when unpleasant affect fades faster than pleasant affect. To detect mechanisms that influence the FAB in the context of death, we measured neuroticism, depression, anxiety, negative religious coping, death attitudes, and complicated grief as potential predictors of FAB for unpleasant/death and pleasant events at 2 points in time. The FAB was robust across older and newer events, which supported the mobilization-minimization hypothesis. Unexpectedly, complicated grief positively predicted FAB, and death avoidant attitudes moderated this relation, such that the Initial Event Affect by Grief interaction was only significant at the highest 3 quintiles of death avoidant attitudes. These results were likely due to moderate grief ratings, which were, along with avoidant death attitudes, related to healthy outcomes in past research. These results implicate complicated grief and death avoidant attitudes as resiliency mechanisms that are mobilized during bereavement to minimize its unpleasant effects.
State machine flattening, a mapping study and tools assessment

State machine formalisms equipped with hierarchy and parallelism allow to compactly model complex system behaviours. Such models can then be transformed into executable code or inputs for model-based testing and verification techniques. Generated artifacts are mostly flat descriptions of system behaviour. Flattening is thus an essential step of these transformations. To assess the importance of flattening, we have defined and applied a systematic mapping process and 30 publications were finally selected. However, it appeared that flattening is rarely the sole focus of the publications and that care devoted to the description and validation of flattening techniques varies greatly. Preliminary assessment of associated tool support indicated limited tool availability and scalability on challenging models. We see this initial investigation as a first step towards generic flattening techniques and scalable tool support, cornerstones of reliable model-based behavioural development.
Gallbladder Pathologies in Kidney Transplant Recipients: Single-Center Experience and a Review of the Literature

Background In patients with end-stage renal disease, cholelithiasis is observed with an increased frequency. In transplant recipients, symptoms might be obscured, which may delay the diagnosis and lead to complications. The aim of our study was to evaluate the frequency of gallbladder pathologies in kidney transplant recipients (KTRs) in the Caucasian population, and to discuss the potential benefits of prophylactic cholecystectomy before kidney transplantation (KT). Material/Methods Data from 434 patients who underwent KT was analyzed. Demographic data along with gallbladder status were collected from the pre-transplantation charts. We compared our results to data from the general Polish population. Results In our analyzed group of KTRs, there were 284 men and 150 women. Complete data, including abdominal ultrasound description, were available in 412 cases. In this group, 36 patients (8.74%) underwent cholecystectomy before KT. Other gallbladder pathologies (gallstones and polyps) were found in 41 patients (9.95%) at pre-transplantation evaluation. The incidence of gallbladder pathologies in KTRs, being mostly cholelithiasis, was higher than in the general Polish population. Conclusions In specific age subgroups of KTRs, the frequency of gallbladder pathologies was higher than in the general population. Prophylactic cholecystectomy may potentially offer benefits in these subgroups of patients.
Ultrafast Cortical Gain Adaptation in the Human Brain by Trial-To-Trial Changes of Associative Strength in Fear Learning

In fear conditioning, more efficient sensory processing of a stimulus (the conditioned stimulus, CS) that has acquired motivational relevance by being paired with an aversive event (the unconditioned stimulus, US) has been associated with increased cortical gain in early sensory brain areas (Miskovic and Keil, 2012). Further, this sensory gain modulation related to short-term plasticity changes occurs independently of aware cognitive anticipation of the aversive US, pointing toward implicit learning mechanisms (Moratti and Keil, 2009). However, it is unknown how quickly the implicit learning of CS–US associations results in the adaptation of cortical gain. Here, using steady-state visually evoked fields derived from human Magnetoencephalography (MEG) recordings in two experiments (N = 33, 17 females and 16 males), we show that stimulus-driven neuromagnetic oscillatory activity increases and decreases quickly as a function of associative strength within three or four trials, as predicted by a computationally implemented Rescorla–Wagner model with the highest learning rate. These ultrafast cortical gain adaptations are restricted to early visual cortex using a delay fear conditioning procedure. Short interval (500 ms) trace conditioning resulted in the same ultrafast activity modulations by associative strength, but in a complex occipito-parieto-temporo-frontal network. Granger causal analysis revealed that reverberating top-down and bottom-up influences between anterior and posterior brain regions during trace conditioning characterized this network. Critically, in both delay and trace conditioning, ultrafast cortical gain modulations as a function of associative strength occurred independently of conscious US anticipation. SIGNIFICANCE STATEMENT In ever-changing environments, learned associations between a cue and an aversive consequence must change under new stimulus–consequence contingencies to be adaptive. What predicts potential dangers now might be meaningless in the next situation. Predictive cues are prioritized, as reflected by increased sensory cortex activity for these cues. However, this modulation also must adapt to altered stimulus–consequence contingencies. Here, we show that human visual cortex activity can be modulated quickly according to ultrafast contingency changes within a few learning trials. This finding extends to frontal brain regions when the cue and the aversive event are separated in time. Critically, this ultrafast updating process occurs orthogonally to aware aversive outcome anticipation and therefore relies on unconscious implicit learning mechanisms.
SINGLE NUCLEOTIDE POLYMORPHISM OF ECCB5 GENE OF MYCOBACTERIUM TUBERCULOSIS COMPLEX ISOLATES FROM SUSPECTED PULMONARY TB PATIENTS IN SURABAYA INDONESIA

Background: Mycobacterium tuberculosis Complex (MTBC) is a group of Mycobacterium that causes tuberculosis (TB). TB is an infectious disease that remains a global health problem. Indonesia is one of the five countries in the world where TB is the most prevalent and became the country with tle second largest rate of TB in 2014 and 2015. MTBC has high pathogenicity that can cause infections in animals and humans. The most common route of transmission is via airborne droplet nuclei and contact with animals or humans infected with TB. MTBC has many virulence factors. One of these factors is EccB5 that is encoded by eccB5 gene. EccB5 is a transmembrane protein-conserved membrane protein and could play a role in inducing damage in host cells, macrophage infection, and may correlate with active disease. The characterization of eccB5 gene needs to be studied to determine the nucleotide sequences, which may be associated with active disease. The aim of this research was to analyze the nuclotide sequences of eccB5 gene of MTBC from suspected pulmonary tuberculosis patients, SNPs of eccB5 gene and possible correlation with the disease, especially in Indonesia. Materials and Methods: Samples were collected from the Tuberculosis Laboratory, Clinical Microbiology of Dr. Soetomo Hospital Surabaya Indonesia. DNA extraction used boiling extraction method and continued nucleic acid amplification using PCR techniques. Primer pairs used eccB5 SK. The positivity of DNA specific revealed amplicon in 1592 bp. PCR product was sequenced by 1st Base (First BASE Laboratories Sdn Bhd, Selangor, Malaysia). The sequence analysis used Genetyx-Win version 10.0 (Genetyx Corporation, Tokyo, Japan). Results: Total isolates of Mycobacterium spp. were 28 and those that showed positive MTBC were 24 isolates and 4 nontuberculosis mycobacteria (NTM) using immunochromatographic test (ICT). The amount of homology from MTBC using blast NCBI was 99%-100%. Two SNPs were found in position c.1277 which revealed replacement of amino acid in 426 of codon position. Conclusion: The sequence of eccB5 gene of MTBC showed high significant homology, while proposed non-synoymous single nucleotide polymorphisms (nsSNP) may associated with clinical outcomes.
Miniaturized Wireless Power Transfer Module Design for Brain Optoelectronic Implant

Optogenetics is a clinical methodology that stimulates genetically modified neurons in the brain using Lasers or µLEDs. The most challenging part of designing an optoelectronic implant is to deliver continuous power to the µLED. To overcome this challenge, a wireless power transfer module is designed and presented in this paper. A miniaturized 10.5 mm×10.5 mm receiver coil has been designed and fabricated using the FR4 substrate and Cu trace as a prototype. Inductive power transmission with magnetic coupling has been utilized to transfer power to the implant at a frequency of 7.2 MHz. The measurement data of the resonant coil shows a return loss (S11) of −9.08 dB, a received power of −15.48 dBm in the air and −18.58 dBm in the muscle media at 5 mm distance with 0 dBm input power. With the design of the proper matching network, the achieved efficiency can be further improved.
Bifunctionally engineered polyethylenimines as efficient DNA carriers and antibacterials against resistant pathogens

In this study, we have designed and developed two series of bifunctional conjugates by tethering polyethylenimine with streptomycin. By varying the amount of streptomycin, conjugates, polyethylenimine-streptomycin, have been synthesized and characterized spectroscopically. Gel electrophoresis assay revealed a slight decrease in the cationic charge density on the conjugates as these retarded the mobility of pDNA at higher w/w ratios. Further, transfection studies showed that both the series of conjugates transfected the mammalian cells efficiently with low-molecular weight polyethylenimine-streptomycin conjugates were more competent (∼9-fold enhancement with respect to native bPEI) exhibiting high cell viability too. Besides, both the series of conjugates displayed excellent antibacterial activity on pathogenic bacteria, even better than native streptomycin on resistant strains. Altogether, these results ensure the promising potential of the projected bifunctional conjugates as safe and efficient gene delivery vectors as well as antibacterials for future biomedical applications.
Upregulated EFNB2 and EPHB4 promotes lung development in a nitrofen-induced congenital diaphragmatic hernia rat model

Congenital diaphragmatic hernia (CDH) is a common congenital malformation associated with high mortality rates, mainly due to pulmonary hypoplasia and persistent pulmonary hypertension following birth. The present study aimed to investigate abnormal lung development in a rat CDH model, and examine temporal and spatial changes in the expression of ephrin type-B receptor 4 (EPHB4) and ephrin-B2 (EFNB2) during fetal lung development, to elucidate the role of these factors during lung morphogenesis. Pregnant rats received nitrofen on embryonic day (E) 8.5 to induce CDH, and fetal lungs were collected on E13.5, E15.5, E17.5, E19.5, and E21.5. The mean linear intercept (MLI) and mean alveolar number (MAN) were observed in fetal lung tissue at E21.5 following hematoxylin and eosin staining. E13.5 fetal lungs were cultured for 96 h in serum-free medium and branch development was observed under a microscope. The gene and protein expression levels of EPHB4 and EFNB2 were assessed by reverse transcription-quantitative polymerase chain reaction analysis, and immunoblotting and immunohistochemistry, respectively. The fetal rat lungs were treated with EFNB2 and the activity of key signaling pathways was assessed. The lung index (lung weight/body weight) at E21.5 was significantly lower in the CDH rats, compared with that in the control fetal rats. The MLI and MAN were also lower in the CDH group. The number of lung terminal buds at E13.5 (embryonic stage), and the lung-explant perimeter and surface were all smaller in the CDH group rats than in the control group at the same age. Pulmonary hypoplasia was observed following 96 h of in vitro culture. No significant differences were found in the expression levels of EFNB2 and EPHB4 between the CDH and control groups at E13.5 (embryonic stage) or E15.5 (pseudoglandular stage), however, EFNB2 and EPHB4 were significantly upregulated at E17.5 (canalicular stage), and at E19.5 and E21.5 (saccular/alveolar stages). EFNB2 stimulated pulmonary branching and EFNB2 supplementation decreased the activity of p38, c-Jun NH2-terminal kinase, extracellular signal-regulated kinase, and signal transducer and activator of transcription. The CDH fetal rats developed pulmonary dysplasia at an early stage of fetal pulmonary development. Upregulated expression of EFNB2 and EPHB4 was observed in the rat lung of nitrofen-induced CDH, and the increased expression of EFNB2 promoted rat lung development in the nitrofen-induced CDH model.
Optimal Delay-Throughput Trade-offs in Mobile Ad-Hoc Networks: Hybrid Random Walk and One-Dimensional Mobility Models

Optimal delay-throughput trade-offs for two-dimensional i.i. d mobility models have been established in [23], where we showed that the optimal trade-offs can be achieved using rate-less codes when the required delay guarantees are sufficient large. In this paper, we extend the results to other mobility models including two-dimensional hybrid random walk model, one-dimensional i.i. d. mobility model and one-dimensional hybrid random walk model. We consider both fast mobiles and slow mobiles, and establish the optimal delay-throughput trade-offs under some conditions. Joint coding-scheduling algorithms are also proposed to achieve the optimal trade-offs.
An Improved PWM Scheme for Three-Level Inverter Extending Operation Into Overmodulation Region With Neutral-Point Voltage Balancing for Full Power-Factor Range

This paper presents a carrier-based pulsewidth modulation (PWM) strategy for a three-level neutral-point-clamped (NPC) inverter that exhibits good control over dc-link capacitor voltages and mitigates low-frequency oscillations in the neutral point (NP) for operation extending into overmodulation region. The overmodulation signals are derived in a generalized way by first determining a bias signal from the zero-sequence injected signals, and then adding the bias with the modulating signals. Such an approach enables seamless transition across different modulation modes covering full modulation depth. It is shown that the incorporation of a signal compression factor generates a nonzero duty ratio for NP connectivity in every PWM cycle in overmodulation region, and thereby creates a room for addition of unbalance compensation signal which can be used to generate neutral current to mitigate existing unbalance in capacitor voltages in the overmodulation region. The compensating signals are in phase with the load currents exhibiting linearity in compensation for full power-factor range. A thorough investigation of the PWM algorithm for operation in both undermodulation and overmodulation with NP voltage regulation is carried out. The performances of the proposed scheme are evaluated through extensive simulation and validated by experiments using a three-level NPC inverter prototype with induction motor load.
Toward a Distributed Trust Management scheme for VANET

A Vehicular Ad hoc NETwork (VANET) is a self-organized network, formed by vehicles and some fixed equipment on roads called Roads Side Units (RSUs). Vehicular communications are expected to share different kinds of information between vehicles and infrastructure. Because of these specifications, securing VANET constitutes a difficult and challenging task that has attracted the interest of many researchers. In a previous work, we proposed a Clustering Mechanism for VANET (CMV) and its inherit Trust management scheme (TCMV) to ensure security of communication among vehicles. CMV organizes vehicles into clusters and elected Cluster Heads (CHs), and allows the clusters maintenance while dealing with velocity. On the other side, TCMV computes the credibility of the message by CH using the reputation of vehicles. However, we found that the value of credibility of the message by CH is not enough to verify if an exchanged message is correct or no. In order to provide a secured vehicle communication and to build reliance communication among vehicles, we propose a distributive trust management scheme for VANET to verify the correctness of the message based on the controlling of the vehicle'behavior by a miner and the credibility of message by a CH.
Optimal Design of Cascade LDPC-CPM System Based on Bionic Swarm Optimization Algorithm

The direct-to-home satellite digital video broadcasting has been fast developed in the past decades, and the continuous phase modulation (CPM) is an alternative modulation scheme with the advantages of constant envelope and high spectral efficiency for the band-limited channel and non-linear amplifiers of the satellite transponder. In this paper, CPM cascaded with irregular low density parity check (LDPC) code is studied. A practical system model with global interleaving is proposed for the optimization of degree distribution of the LDPC codes, and code word design is obtained with a modified progressive edge-growth algorithm. Simulation results combined with theoretical analysis clarify the effect of signal-to-noise ratios and two important CPM parameters (modulation index <inline-formula> <tex-math notation="LaTeX">${h}$ </tex-math></inline-formula> and correlation length <inline-formula> <tex-math notation="LaTeX">${L}$ </tex-math></inline-formula>), providing guidance for system design. Simulation on bit error rate performance show the validity of the proposed algorithm. This paper systematically solves the optimal system design of cascade irregular LDPC-CPM and is of great significance for future satellite broadcasting.
Borg CR-10 scale as a new approach to monitoring office exercise training.

BACKGROUND There are many potential training exercises for office workers in an attempt to prevent musculoskeletal disorders. However, to date a suitable tool to monitor the perceived exertion of those exercises does not exist. OBJECTIVE The primary objective of this study was to examine the validity and reliability of the Borg CR-10 scale to monitor the perceived exertion of office exercise training. METHODS The study involved 105 staff members employed in a government office with an age range from 25 to 50 years. The Borg CR-10 scale was self-administered two times, with an interval of two weeks in order to evaluate the accuracy of the original findings with a retest. Face validity and content validity were also examined. RESULTS Reliability was found to be high for the Borg CR-10 scale (0.898). Additionally a high correlation between the Borg CR-10 scale and Visual Analog Scale (VAS) was identified (rs = 0.754, P < 0.01). CONCLUSIONS This study found the Borg CR-10 scale to be a reliable and valid tool for monitoring the perceived exertion of office exercise training and may potentially be useful for occupational therapists to measure physical activity intensity levels.
Memory Inflation Drives Tissue-Resident Memory CD8+ T Cell Maintenance in the Lung After Intranasal Vaccination With Murine Cytomegalovirus

Tissue-resident memory T (TRM) cells provide first-line defense against invading pathogens encountered at barrier sites. In the lungs, TRM cells protect against respiratory infections, but wane more quickly than TRM cells in other tissues. This lack of a sustained TRM population in the lung parenchyma explains, at least in part, why infections with some pathogens, such as influenza virus and respiratory syncytial virus (RSV), recur throughout life. Intranasal (IN) vaccination with a murine cytomegalovirus (MCMV) vector expressing the M protein of RSV (MCMV-M) has been shown to elicit robust populations of CD8+ TRM cells that accumulate over time and mediate early viral clearance. To extend this finding, we compared the inflationary CD8+ T cell population elicited by MCMV-M vaccination with a conventional CD8+ T cell population elicited by an MCMV vector expressing the M2 protein of RSV (MCMV-M2). Vaccination with MCMV-M2 induced a population of M2-specific CD8+ TRM cells that waned rapidly, akin to the M2-specific CD8+ TRM cell population elicited by infection with RSV. In contrast to the natural immunodominance profile, however, coadministration of MCMV-M and MCMV-M2 did not suppress the M-specific CD8+ T cell response, suggesting that progressive expansion was driven by continuous antigen presentation, irrespective of the competitive or regulatory effects of M2-specific CD8+ T cells. Moreover, effective viral clearance mediated by M-specific CD8+ TRM cells was not affected by the coinduction of M2-specific CD8+ T cells. These data show that memory inflation is required for the maintenance of CD8+ TRM cells in the lungs after IN vaccination with MCMV.
Stress in Regulation of GABA Amygdala System and Relevance to Neuropsychiatric Diseases

The amygdala is an almond-shaped nucleus located deep and medially within the temporal lobe and is thought to play a crucial role in the regulation of emotional processes. GABAergic neurotransmission inhibits the amygdala and prevents us from generating inappropriate emotional and behavioral responses. Stress may cause the reduction of the GABAergic interneuronal network and the development of neuropsychological diseases. In this review, we summarize the recent evidence investigating the possible mechanisms underlying GABAergic control of the amygdala and its interaction with acute and chronic stress. Taken together, this study may contribute to future progress in finding new approaches to reverse the attenuation of GABAergic neurotransmission induced by stress in the amygdala.