Miracle-dz/UniFAR-FTUs huggingface dataset:
Fatty-acid-binding protein 5 promotes cell proliferation and invasion in oral squamous cell carcinoma.

BACKGROUND Oral squamous cell carcinoma (OSCC) is one of the most malignant neoplasms worldwide, and the molecular mechanism of oral tumorigenesis is still unclear. Fatty-acid-binding protein 5 (FABP5) was found in our previous study to be upregulated in oral squamous cell carcinomas by proteomic analysis. The implications of FABP5 overexpression in oral cancer progression have not yet been elucidated. MATERIALS AND METHODS In this study, the recombinant adeno-associated virus vectors were used to deliver and increase the expression of FABP5 in human OSCC cell lines. U6 promoter-driven short-hairpin RNA (shRNA)-triggered RNA interference was used to block FABP5 gene expression. Transwell Matrigel invasion assay, MTS cell proliferation assay, reverse transcription-polymerase chain reaction, Western blot, and gelatin zymography analysis were used to investigate the effects of FABP5 on cell invasion, growth, and matrix metalloproteinase (MMP) production. RESULTS Overexpression of FABP5 in oral cancer cells increased cell proliferation and invasiveness by increasing the expression of MMP-9. Silencing FABP5 with shRNA significantly suppressed cell proliferation, MMP-9 activities, and invasiveness. CONCLUSION Our study provides the first evidence that FABP5 expression modulated MMP-9 production and the invasive behavior of oral cancer cells and suggests that FABP5 may provide novel targets for therapeutic intervention.
Acute confabulatory psychosis: A rare form of unipolar mania?

Emil Kraepelin's paper on false memories (Erinnerungsfälschungen) was probably the most extensive and incisive analysis of these phenomena ever made. Using his views as a starting point, the concept of confabulation is then defined in a Kraepelin‐oriented manner, making it also applicable to the phantastic false memories found in some rarer forms of functional psychotic illness. Kraepelin and Leonhard have been preeminent in their concern with such clinical states; thus, Leonhard's confabulatory euphoria and confabulatory paraphrenia can be symptomatically and syndromally linked up with points of view on paranoid mania and confabulatory paraphrenia held by Kraepelin. An “ideally typical” case is also presented to highlight some of the difficulties involved in trying to distinguish mania from schizophrenia when the clinical picture is dominated by phantastic “functional” confabulations.
Alpha 2 adrenoceptor agonists and anaesthesia.

The clinical application of alpha 2 adrenergic agonists for anaesthesia has no precedent in our discipline. New classes of anaesthetic drugs, or even new members of an existing class, are usually promoted by an immense pharmaceutical industry effort. The alpha, adrenergic agonists have arrived on the scene with no hyperbole: these drugs will succeed (or fail) on their own merits. In addition, more is known about the underlying mechanism of action of these compounds than is known about all other anaesthetic agents. In this review we have concentrated on basic, applied and clinical pharmacology of these agents as they relate to anaesthesia.
The risks of long‐term neuroleptic treatment of schizophrenia: especially depression and akinesia

Most extrapyramidal side effects of neuroleptics are easily recognized and cause little problem in diagnosis and treatment. The exceptions are those which mimic psychopathology: akathesia and akinisia. Akathesia resembles anxiety. Akinesia is similar to depression, residual schizophrenia, and demoralization. Differential diagnosis may require interruption of the neuroleptic. Whether neuroleptics can cause depression not associated with akinesia has never been established. The first obligation of the clinician is to be sure that the depressive symptoms are not concomitants of akinesia, before treating with antidepressants which may not be the appropriate treatment. There is no convincing evidence that one neuroleptic drug is more likely to cause akinesia than another, although this had not been studied extensively using a broad definition of akinesia.
The relationship between extension of the metatarsophalangeal joint and sprint time for 100 m Olympic athletes

Abstract Selected kinematic variables of the foot segments and the metatarsophalangeal (MTP) joint were investigated in relation to sprinting performance among 100 m sprint athletes at the 2000 Summer Olympic Games. It was hypothesized that the kinematics of the MTP joint, and forefoot and rearfoot segments, are related to sprint performance for both male and female athletes. Kinematic sagittal plane data were collected using two digital video cameras recording at 120 fields per second. It was determined that faster male sprinters experienced higher maximal rates of MTP extension, and faster female sprinters touch down with higher posterior sole angles and take off with lower posterior sole angles.
A Comprehensive Antibody Panel for Immunohistochemical Analysis of Formalin-Fixed, Paraffin-Embedded Hematopoietic Neoplasms of Mice: Analysis of Mouse Specific and Human Antibodies Cross-Reactive with Murine Tissue

Immunohistochemistry is an indispensable tool in human pathology enabling immunophenotypic characterization of tumor cells. Immunohisto-chemical analyses of mouse models of human hematopoietic neoplasias have become an important aspect for comparison of murine entities with their human counterparts. The aim of this study was to establish a diagnostic antibody panel for analysis of murine lymphomas/leukemias, useful in formalin-fixed/paraffin-embedded tissue. Overall, 48 antibodies (4 rabbit monoclonal, 12 rabbit polyclonal, 2 goat polyclonal, 11 rat, and 19 mouse monoclonal), which were either mouse-specific (14) or cross-reactive with murine tissue (34) were tested for staining quality and diagnostic value in 468 murine hematopoietic neoplasms. Specific staining was achieved with 29 antibodies, of which 18 were human antibodies cross-reactive with murine tissue. Only 23 (B220, BCL-2, BCL-6, CD117, CD138 (2×), CD3 (2×), CD43, CD45, CD5, CD79αcy, cyclin D1, Ki-67 (2×), Mac-3, Mac-2, lysozyme, mast cell tryptase, MPO, Pax-5, TdT, and TER-119) were regarded as valuable for diagnostic evaluation. Immunohistochemistry was also established in an automated immunostainer for high throughput analysis. The antibody panel developed is useful for the classification of murine lymphomas and leukemias analyzed, and a valuable tool for human and veterinary pathologists involved in the diagnostic interpretation of murine models of hematopoietic neoplasias.
Stalking Victimization, Labeling, and Reporting

Using the National Crime Victimization Survey 2006 Stalking Victimization Supplement (NCVS-SVS) and guided by Greenberg and Ruback’s social influence model, this study examines the effects of individual (e.g., severity, sex, victim–offender relationship) and contextual (e.g., location) factors on stalking victimization risk, victim labeling and help seeking, and victim and third-party police contacts. Logistic regression results suggest individual and contextual characteristics matter. Consistent with prior research and the theoretical model, the positive effects of severity and sex (female) were significant across all dependent variables, whereas the interaction effect of victim–offender relationship and location held only for third-party police contacts.
p63 in prostate biology and pathology

The identification of stem cells and differentiation programs regulating the development and maintenance of the normal prostate epithelium is essential for the identification of the cell type(s) and molecular alterations involved in the development and propagation of prostate cancer (CaP). The p53‐homologue p63 is highly expressed in normal prostate basal cells and is a clinically useful biomarker for the diagnosis of CaP. Importantly, p63 has been shown to play a critical role in prostate development. Recent experimental evidence also suggests that this gene is essential for normal stem cell function in the prostate as well as other epithelial organs. Future studies aimed at better defining the role of p63 in the renewal of the adult prostate epithelium are likely to shed new light on the mechanisms involved in prostate carcinogenesis. J. Cell. Biochem. 103: 1354–1368, 2008. © 2007 Wiley‐Liss, Inc.
An icosahedral virus as a fluorescent calibration standard: a method for counting protein molecules in cells by fluorescence microscopy

The ability to replace genes coding for cellular proteins with DNA that codes for fluorescent protein-tagged versions opens the way to counting the number of molecules of each protein component of macromolecular assemblies in vivo by measuring fluorescence microscopically. Converting fluorescence to absolute numbers of molecules requires a fluorescent standard whose molecular composition is known precisely. In this report the construction, properties, and mode of using a set of fluorescence calibration standards are described. The standards are based on an icosahedral virus engineered to contain exactly 240 copies of one of seven different fluorescent proteins. Two applications of the fluorescent standards to counting molecules in the human parasite Toxoplasma gondii are described. Methods for improving the preciseness of the measurements and minimizing potential inaccuracies are emphasized. Lay Abstract A broad goal of modern biology is to understand how the machines within living cells work. It is nowadays routine to identify the individual protein components of a machine, but not yet straightforward to tell how many copies of each component are needed to build a functional assembly. In many types of cells it is now possible to substitute for the native proteins within cells altered versions that are fluorescent. If one knew how much fluorescence is generated by a single molecule of the altered protein, then one could use a light microscope to count the number of copies of the protein in a cellular machine by simply measuring the total fluorescence coming from that part of the cell. This paper describes the construction and methods for using a set of fluorescent virus particles that can be used to determine how much fluorescence is contributed by one molecule of fluorescent protein. The virus particles were chosen for this role because the particular icosahedral symmetry of their structure guarantees that each particle contains exactly 240 copies of one fluorescent protein.
Initial recommendations for higher‐tier risk assessment protocols for bumble bees, Bombus spp. (Hymenoptera: Apidae)

Global declines of bumble bees and other pollinator populations are of concern because of their critical role for crop production and maintenance of wild plant biodiversity. Although the consensus among scientists is that the interaction of many factors, including habitat loss, forage scarcity, diseases, parasites, and pesticides, potentially plays a role in causing these declines, pesticides have received considerable attention and scrutiny. In response, regulatory agencies have introduced more stringent pollinator testing requirements for registration and reregistration of pesticides, to ensure that the risks to pollinators are minimized. In this context, guidelines for testing bumble bees (Bombus spp.) in regulatory studies are not yet available, and a pressing need exists to develop suitable protocols for routine higher‐tier studies with these non‐Apis sp. , social bees. To meet this need, Bayer CropScience LP, Syngenta Crop Protection LLC US, and Valent USA. Corporation organized a workshop bringing together a group of global experts on bumble bee behavior, ecology, and ecotoxicology to discuss and develop draft protocols for both semi‐field (Tier II) and field (Tier III) studies. The workshop was held May 8–9, 2014, at the Bayer Bee Care Center, North Carolina, USA. The participants represented academic, consulting, and industry scientists from Europe, Canada, the United States, and Brazil. The workshop identified a clear protection goal and generated proposals for basic experimental designs, relevant measurements, and endpoints for both semifield (tunnel) and field tests. These initial recommendations are intended to form the basis of discussions to help advance the development of appropriate protocol guidelines. Integr Environ Assess Manag 2016;12:222–229. © 2015 The Authors. Integrated Environmental Assessment and Management Published by SETAC
Negative (eccentric) work. II. Physiological responses to walking uphill and downhill on a motor-driven treadmill.

A comparison has been made between walking uphill (+ W) and downhill (-W) for 1 hr on a motor-driven treadmill on 7 healthy male subjects aged 21-41 years. Measurements included: oxygen intake ([Vdot]O2) and CO2 output ([Vdot]CO2). minute ventilation ([Vdot]E), tidal volume ([Vdot]T), mixed venous (P[vtilde]CO2) and alveolar (PACO2)PCO2, cardiac output ([Qdot]) and frequency (fH), tympanic temperature (Tty) sweat rate (S) and blood lactic acid (LA) concentration.
MIPS: a database for genomes and protein sequences

The Munich Information Center for Protein Sequences (MIPS-GSF), Martinsried near Munich, Germany, develops and maintains genome oriented databases. It is commonplace that the amount of sequence data available increases rapidly, but not the capacity of qualified manual annotation at the sequence databases. Therefore, our strategy aims to cope with the data stream by the comprehensive application of analysis tools to sequences of complete genomes, the systematic classification of protein sequences and the active support of sequence analysis and functional genomics projects. This report describes the systematic and up-to-date analysis of genomes (PEDANT), a comprehensive database of the yeast genome (MYGD), a database reflecting the progress in sequencing the Arabidopsis thaliana genome (MATD), the database of assembled, annotated human EST clusters (MEST), and the collection of protein sequence data within the framework of the PIR-International Protein Sequence Database (described elsewhere in this volume). MIPS provides access through its WWW server (http://www.mips.biochem.mpg.de) to a spectrum of generic databases, including the above mentioned as well as a database of protein families (PROTFAM), the MITOP database, and the all-against-all FASTA database.
Performance Assessment of IR-UWB Body Area Network (BAN) Based on IEEE 802.15.6 Standard

Performance of impulse radio-based ultrawideband (IR-UWB) communications in wireless body area networks is investigated using the dedicated IEEE 802.15. 6 standard. An IR-UWB transceiver system is implemented for both on-off keying and differential binary phase-shift keying modulations. Bit error rates are determined from measurements for different on-body links with different data rates. It is shown that using a 25-dB-gain low noise amplifier (LNA) at the receiver, reaching an uncoded bit error rate BER of 10-3 was not possible for some links operating at higher data rates. Power and energy consumption issues are then addressed, and results in terms of required pJ/bit to achieve a certain quality of communication are given and discussed.
The Correlation between Ion Beam/Material Interactions and Practical FIB Specimen Preparation

The focused ion beam (FIB) tool has been successfully used as both a stand alone analytical instrument and a means to prepare specimens for subsequent analysis by SEM, TEM, SIMS, XPS, and AUGER. In this work, special emphasis is given to TEM specimen preparation by the FIB lift-out technique. The fundamental ion/solid interactions that govern the FIB milling process are examined and discussed with respect to the preparation of electron transparent membranes. TRIM, a Monte Carlo simulation code, is used to physically model variables that influence FIB sputtering behavior. The results of such computer generated models are compared with empirical observations in a number of materials processed with an FEI 611 FIB workstation. The roles of incident ion attack angle, beam current, trench geometry, raster pattern, and target-material-dependent removal rates are considered. These interrelationships are used to explain observed phenomena and predict expected milling behaviors, thus increasing the potential for the FIB to be used more efficiently with reproducible results.
Pharmacokinetics of Subcutaneously Injected Tritiated Insulin: Effects of Exercise

The acute exercise of muscle in insulin-dependent diabetic patients is frequently associated with metabolic complications, i.e. in poorly controlled insulin-deficient patients, physical activity can induce a further deterioration of the metabolic and hormonal situation,and, above all, in normoglycemic, well-controlled patients following the subcutaneous injection of insulin, muscular exercise can precipitate hypoglycemia. The latter phenomenon was first described by Lawrence (Figure 1) and Burger and Kramer only shortly after insulin had become available in clinical medicine; both authors ascribed it to a potentiation of the hypoglycemic effect of subcutaneously injected insulin by exercise. As a consequence, exercise was recommended in the treatment of juvenile diabetics because it should reduce the daily insulin requirements that were generally felt to be beneficial to the patient.With regard to the mechanism of the potentiation of the hypoglycemic effect of subcutaneously injected insulin by exercise, Kawamori and Vranic have demonstrated in pancreatectomized dogs after the injection of a mixture of regular and protamine zinc (PZI) insulin that early during exercise there was a substantial increase of circulating insulin levels. An exerciseinduced increase of circulating insulin has also been observed in three diabetic patients treated with long-acting insulin preparations,which contrasts to the physiologic fall of serum insulin during physical exercise.This observation suggested an increased mobilization of insulin from its subcutaneous depot during treadmill running in diabetic insulin-treated dogs. The exercise-induced rise in plasma insulin was, however, variable, the peak being of short duration, and the magnitude of the blood glucose fall did not correlate with the rise of insulin levels. When external gamma-counting above the injection site following the subcutaneous administration of radioiodinated insulin was used in man to assess the absorption rate of insulin, Joiner and Binder did not observe any effect of physical activity on the apparent mobilization of the tracer, whereas more recently Koivisto and Felig have demonstrated an accelerated disappearance of radioactivity from the leg injection site in patients subjected to bicycle exercise. * In order to study the effect of exercise on the mobilization of exogenous insulin we have injected semisynthetic [H]insulin in rats and diabetic patients and followed its circulating levels at rest and during exercise. (In previous experiments on rats we have shown that treadmill exercise does potentiate the hypoglycemic effect of subcutaneously injected unlabeled insulin, analogous to observations in normal and diabetic man.-)
Basal Ganglia Modeling in Healthy and Parkinson's Disease State. II. Network-based Multi-Units Simulation

Parkinson's disease (PD) is treated by means of deep brain stimulation (DBS), a therapy based on the injection of current on a regular basis into the basal ganglia, a set of small subcortical nervous system nuclei. DBS is effective in relief of motor symptoms and leads to a notable reduction of drug dose. From an engineering viewpoint, it also raises several optimization-related issues, like energy consumption minimization and stimulation effectiveness. How DBS really works, however, is still unknown, even though several hypotheses and experiments have been reported in literature. To shed some light on the function of DBS, we focused in [1] on those brain nuclei involved in the genesis of PD motor symptoms and developed for them conductance-based models to mimic quantitative data from different in vitro experiments. Now, we use such models in a network scheme to reproduce the main existing anatomical connections between and within the basal ganglia and the resulting macroscopic behaviors reported in literature both for normal and Parkinsonian state. The overall model is proposed as a simulatioParkinson's disease (PD) is treated by means of deep brain stimulation (DBS), a therapy based on the injection of current on a regular basis into the basal ganglia, a set of small subcortical nervous system nuclei. DBS is effective in relief of motor symptoms and leads to a notable reduction of drug dose. From an engineering viewpoint, it also raises several optimization-related issues, like energy consumption minimization and stimulation effectiveness. How DBS really works, however, is still unknown, even though several hypotheses and experiments have been reported in literature. To shed some light on the function of DBS, we focused in (S. Santaniello et al., 20007) on those brain nuclei involved in the genesis of PD motor symptoms and developed for them conductance-based models to mimic quantitative data from different in vitro experiments. Now, we use such models in a network scheme to reproduce the main existing anatomical connections between and within the basal ganglia and the resulting macroscopic behaviors reported in literature both for normal and Parkinsonian state. The overall model is proposed as a simulation instrument to better understand the functional mechanisms currently explaining the basal ganglia physiology and the cellular effects of DBS.n instrument to better understand the functional mechanisms currently explaining the basal ganglia physiology and the cellular effects of DBS.
Facet Orientation in the Thoracolumbar Spine: Three-dimensional Anatomic and Biomechanical Analysis

Study Design. Thoracolumbar facet orientations were measured and analyzed. Objectives. To establish a comprehensive database for facet orientation in the thoracolumbar vertebrae and to determine the normal human condition. Summary of Background Data. Most studies on facet orientation have based their conclusions on two-dimensional measurements, in small samples or isolated vertebrae. The amount of normal asymmetry in facet orientation is poorly addressed. Methods. Transverse and longitudinal facet angles were measured directly from 240 human vertebral columns (males/females, blacks/whites). The specimens’ osteologic material is part of the Hamann-Todd Osteological Collection housed at the Cleveland Museum of Natural History (Cleveland, OH). A total of 4,080 vertebrae (T1–L5) from the vertebral columns of individuals 20 to 80 years of age were measured, using a Microscribe three-dimensional apparatus (Immersion Co., San Jose, CA). Data were recorded directly on computer software. Statistical analysis included paired t tests and analysis of variance. Results. Facet orientation is independent of gender, age, and ethnic group. Asymmetry in facet orientation is found in the thorax. All thoracolumbar facets are positioned in an oblique plane. In the transverse plane, all facets from T1 to T11 are positioned with an anterior inclination of approximately 25° to 30° from the frontal plane. The facets of T12–L2 are oriented closer to the midsagittal plane of the vertebral body (mean range, 25.89°–33.87°), while the facets of L3–L5 are oriented away from that plane (mean range, 40.40°–56.30°). Facettransverse orientation at the thoracolumbar junction is highly variable (∼80% with ∼101° and ∼20% with 35°). All facets are oriented more vertically from T1 (∼150°) to L5 (∼170°). The facet sagittal orientations of the lumbar zygoapophyseal joints are not equivalent. Conclusions. Asymmetry in facet orientation is a normal characteristic in the thorax.