Miracle-dz/UniFAR-FTUs huggingface dataset:
Lack of adverse effects in pregnant/lactating female rats and their offspring following pre‐ and postnatal exposure to ELF magnetic fields

We have recently reported that exposure of pregnant rats to 60 Hz at field strengths up to 0.5 mT during the entire period of pregnancy did not induce any biologically significant effects on both pregnant dams and embryo‐fetal development. The present study was carried out to investigate the potential effects of gestational and lactational MF exposure on pregnancy, delivery, and lactation of dams and growth, behavior, and mating performance of their offspring in rats. Timed‐pregnant female Sprague–Dawley (SD) rats (24/group) received continuous exposure to 60 Hz magnetic field (MF) at field strengths of 0 (sham control), 5 μT, 83.3 μT, or 0.5 mT. Dams received MF or sham exposures for 21 h/day from gestational day 6 through lactational day 21. Experimentally generated MF was monitored continuously throughout the study. No exposure‐related changes in clinical signs, body weight, food consumption, pregnancy length, and necropsy findings were observed in dams. Parameters of growth, behavior, and reproductive performance of offspring showed no changes related to MF exposure. There were no adverse effects on embryo‐fetal development of F2 offspring from dams exposed to MF. In conclusion, exposure of pregnant SD rats to 60 Hz at field strengths up to 0.5 mT from gestational day 6 to lactational day 21 did not produce biologically significant effects in dams, F1 offspring, or F2 fetuses. Bioelectromagnetics 25:236–244, 2004. © 2004 Wiley‐Liss, Inc.
The impact of fatherhood on treatment response for men with co-occurring alcohol dependence and intimate partner violence

Objective: The role of fathers in the lives of children has gained increasing attention over the last several decades, however, studies that specifically examine the parenting role among men who are alcohol dependent and have co-occurring intimate partner violence (IPV) have been limited. This brief report is intended to highlight the need to develop and focus interventions for men with co-occurring substance abuse and IPV with an emphasis on their roles as fathers. Method: Sixty-nine men who participated in a randomized comparison study of a coordinated substance abuse and domestic violence treatment program (SADV) and Twelve Step Facilitation (TSF) provided information about whether they were fathers. Analysis of covariance was used to assess the impact of fatherhood on the outcomes of intimate partner violence and alcohol use during the 12 weeks of treatment. Results: There was a significant interaction between type of treatment (SADV vs. TSF) and fatherhood. SADV resulted in significantly less IPV and use of alcohol over the 12 weeks of treatment than TSF for men without children. There were no significant differences between SADV and TSF for men who were fathers. Conclusion and Scientific Significance: Results indicate a need to further explore the role of fatherhood for men with co-occurring substance abuse and IPV and development of specialized treatments that may improve treatment outcomes for fathers.
Fifth metatarsal fractures among male professional footballers: a potential career-ending disease

Background There is little information about Metatarsal Five (MT-5) fractures for specific sports. Objective To study the occurrence, the imaging characteristics, the lay-off times and healing problems of MT-5 fractures among male footballers. Methods Sixty-four European elite teams were monitored from 2001 to 2012. x-Rays were collected and classified by the Torg criteria. Results Of 13 754 injuries, 0.5% (67) proved to be MT-5 fractures. Their incidence was 0.04 injuries/1000 h of exposure. A team of 25 players might thus expect an MT-5 fracture every fifth season. Of these fractures, 67% (38) were primary and 33% were refractures. One of the 38 primary fractures was an avulsion of the tuberosity; all the others (97%) located towards the base. In total, 32% of the players with MT-5 fracture were younger than 21 years, 40% of the fractures occurred during the preseason and 45% of the players had prodromal symptoms. In total, 54% of the initial x-rays were classified as Torg type II (stress fractures), and 46% were classified as Torg type I (acute type). After surgical treatment the fractures healed faster, compared with conservative treatment (75% vs 33%, p<0.05). There was no significant difference in lay-off days between players that had been operated, and those that had not (80 vs 74 days, p=0.67). Conclusions The majority of MT-5 fractures are stress fractures, and mainly occur among young players. There are frequent healing problems, which might be explained by the stress nature of the injury. After surgery there are less healing problems, compared with those in conservative treatment.
Growth Performance, Haematological Indices and Cost Benefits of Growing Pigs Fed Cassava Peel Meal Diets Supplemented With Allzyme® SSF

Effect of replacing dietary maize with cassava peel meal (CPM) supplemented with Allzyme® SSF (SSF) on the growth performance, haematological indices and cost benefits of growing pigs was investigated. Chemical composition of CPM and diets used were determined using standard procedures. Twenty-four growing pigs with average weight of 14.0+0.25kg were allotted to 6 dietary treatments whereby CPM replaced maize at 0, 50 and 100% levels with SSF supplementation at 0g/100kg and 20g/100kg of diet. Each treatment was replicated 4 times while the experiment lasted 8 weeks. Data were collected on daily feed intake (DFI), daily weight gain (DWG) and feed conversion ratio (FCR); feed cost/weight gain and feed cost/day were calculated. Blood samples collected through jugular vein puncture were analyzed for haematological indices such as white blood cells (WBC), red blood cell and platelet count. The CPM diets with or without SSF gave comparable (P>0.05) pigs’ growth performance to what was obtained for pigs on the maize based diets in terms of DFI (1.26 – 1.33kg), DWG (0.32 – 0.40kg) and FCR (3.13 – 4.03). Values for WBC were within normal range, though they had significant (p 0.05) by CPM inclusion. Feed cost/kg diet (N54.11 – N74.39) and feed cost/weight gain (N195.33 – N244.65) reduced insignificantly (P>0.05) with CPM inclusion in the diets of pigs. It was concluded that cassava peel meal can totally replace maize with or without enzyme supplementation in the diets of growing pigs for optimal performance at reduced cost.
Urinary proteomic biomarkers to predict cardiovascular events

We have previously demonstrated associations between the urinary proteome profile and coronary artery disease (CAD) in cross‐sectional studies. Here, we evaluate the potential of a urinary proteomic panel as a predictor of CAD in the hypertensive atherosclerotic cardiovascular disease (HACVD) substudy population of the Anglo‐Scandinavian Cardiac Outcomes Trial study.
Effects of The Coach Approach Intervention on Adherence to Exercise in Obese Women

The link between physical activity and weight loss has precipitated interest in interventions to foster adherence to exercise. It has been suggested that treatment effects, when significant, should be analyzed to determine theory-based mediators. This research assessed possible mediation of changes in Physical Self-Concept, Exercise Self-Efficacy, Total Mood Disturbance, and Body Areas Satisfaction scores on the relationship between exercise session attendance and participation by obese women in a 6-month treatment based on tenets of social cognitive theory (n = 73) or a control condition (n = 64). Participation in the treatment was associated with significantly greater exercise session attendance and significantly greater improvements in Physical Self-Concept, Exercise Barriers Self-Efficacy, and Body Areas Satisfaction. Overall, changes in the assessed psychological factors demonstrated significant mediation of the association between group membership and exercise session attendance, R2 = . 23, with only change in Physical Self-Concept scores providing a significant unique contribution. Extensions of this research across different sample types, and with longer durations, were suggested to refine theory and, ultimately, improve exercise adherence treatments.
OmpR Regulates the Two-Component System SsrA-SsrB in Salmonella Pathogenicity Island 2

ABSTRACT Salmonella pathogenicity island 2 (SPI-2) encodes a putative, two-component regulatory system, SsrA-SsrB, which regulates a type III secretion system needed for replication inside macrophages and systemic infection in mice. The sensor and regulator homologs,ssrAB (spiR), and genes within the secretion system, including the structural gene ssaH, are transcribed after Salmonella enters host cells. We have studied the transcriptional regulation of ssrAB and the secretion system by using gfp fusions to the ssrA andssaH promoters. We found that early transcription ofssrA, after entry into macrophages, is most efficient in the presence of OmpR. An ompR mutant strain does not exhibit replication within cultured macrophages. Furthermore, footprint analysis shows that purified OmpR protein binds directly to thessrA promoter region. We also show that minimal medium, pH 4.5, induces SPI-2 gene expression in wild-type but notompR mutant strains. We conclude that the type III secretion system of SPI-2 is regulated by OmpR, which activates expression of ssrA soon after Salmonella enters the macrophage.
Lamivudine for chronic delta hepatitis

Chronic delta hepatitis is a severe form of chronic liver disease caused by hepatitis delta virus (HDV) infection superimposed on chronic hepatitis B or the hepatitis B surface antigen (HBsAg) carrier state. Therapy of delta hepatitis is currently unsatisfactory. We have evaluated lamivudine (3‐thiacytidine), an oral nucleoside analogue with marked effects against hepatitis B, as therapy in 5 patients with chronic hepatitis D. Five men, ages 38 to 65 years, were treated. All had HBsAg, antibody to HDV, and HDV RNA in serum, as well as persistent elevations in alanine aminotransferase (ALT) levels and liver histology showing severe chronic hepatitis with fibrosis or cirrhosis. Lamivudine was given in a dose of 100 mg orally daily for 12 months. Patients were monitored carefully and tested for HBsAg, HBV‐DNA and HDV‐RNA levels serially during the year of treatment and for 6 months thereafter. Liver biopsies were performed before therapy and repeated after 1 year. Serum levels of HBV DNA fell rapidly in all 5 patients, becoming undetectable even by polymerase chain reaction (PCR) in 4. However, all 5 patients remained HBsAg‐ and HDV‐RNA–positive, and serum ALT levels and liver histology did not improve. All patients tolerated therapy well. When lamivudine was stopped, HBV‐DNA levels returned to pretreatment values without a change in disease activity. Lamivudine is a potent inhibitor of HBV‐DNA replication, but does not improve disease activity or lower HDV‐RNA levels in patients with chronic delta hepatitis.
Quantitation of bovine papilloma viral DNA in viral-induced tumors

Bovine papilloma virus (BPV) DNA was labeled in vitro under conditions of repair synthesis and subsequently used as a "probe" in DNA-DNA reassociation studies to detect BPV-specific DNA sequences in a viral-induced calf meningioma and hamster fibroma. In vitro labeled BPV DNA had denaturation characteristics expected for duplex DNA and denatured DNA reassociated with apparent second-order kinetics. Analysis of in vitro labeled BPV DNA reassociation rates in the presence of excess tumor DNA revealed that the calf meningioma contained approximately 700 to 800 BPV genome equivalents per diploid cell whereas the hamster fibroma contained about 150 incomplete BPV genome equivalents per diploid cell. Thermal denaturation of in vitro labeled BPV DNA which reassociated in the presence of the two tumor DNA preparations indicated less than 1.5% base pair mismatching.
A Downshift in Temperature Activates the High Osmolarity Glycerol (HOG) Pathway, Which Determines Freeze Tolerance in Saccharomyces cerevisiae*

The molecular mechanisms that enable yeast cells to detect and transmit cold signals and their physiological significance in the adaptive response to low temperatures are unknown. Here, we have demonstrated that the MAPK Hog1p is specifically activated in response to cold. Phosphorylation of Hog1p was dependent on Pbs2p, the MAPK kinase (MAPKK) of the high osmolarity glycerol (HOG) pathway, and Ssk1p, the response regulator of the two-component system Sln1p-Ypd1p. However, Sho1p was not required. Interestingly, phosphorylation of Hog1p was stimulated at 30 °C in cells exposed to the membrane rigidifier agent dimethyl sulfoxide. Moreover, Hog1p activation occurred specifically through the Sln1 branch. This suggests that Sln1p monitors changes in membrane fluidity caused by cold. Quite remarkably, activation of Hog1p at low temperatures affected the transcriptional response to cold shock. Indeed, the absence of Hog1p impaired the cold-instigated expression of genes for trehalose- and glycerol-synthesizing enzymes and small chaperones. Moreover, a downward transfer to 12 or 4 °C stimulated the overproduction of glycerol in a Hog1p-dependent manner. However, hog1Δ mutant cells showed no growth defects at 12 °C as compared with the wild type. On the contrary, deletion of HOG1 or GPD1 decreased tolerance to freezing of wild-type cells preincubated at a low temperature, whereas no differences could be detected in cells shifted directly from 30 to –20 °C. Thus, exposure to low temperatures triggered a Hog1p-dependent accumulation of glycerol, which is essential for freeze protection.
Blood miRNA expression pattern is a possible risk marker for natalizumab-associated progressive multifocal leukoencephalopathy in multiple sclerosis patients

Background: Natalizumab has shown its efficacy in reducing multiple sclerosis (MS) relapses and progression of disability; however, it has been associated with an increased risk of developing progressive multifocal leukoencephalopathy (PML). The differential expression of microRNA (miRNA), the small non-coding RNAs that regulate gene expression, in natalizumab-treated patients has been reported and miRNA have also been described as good candidates for disease biomarkers. Objective: To characterize the effect of natalizumab therapy on the miRNA expression pattern and to search for miRNAs that can predict PML on an individual basis. Methods: The expression of 754 microRNAs was measured in blood samples from 19 relapsing–remitting MS patients at three time points during natalizumab therapy, using TaqMan OpenArray panels. Two patients included in this study developed PML after more than 2 years of therapy. Results: We found that the expression level of three miRNAs (let-7c, miR-125a-5p and miR-642) was affected after 6 months of therapy (t6). Furthermore, we observed a differential expression of another three miRNAs (miR-320, miR-320b and miR-629) between the PML and non-PML groups after 12 months of treatment (t12); and a positive correlation was found between therapy time and the expression of miR-320. Conclusions: Natalizumab modified the expression levels of three miRNAs after a 6-month treatment. We suggest miR-320, miR-320b and miR-629 as possible biomarkers for individual PML risk assessment.
Factors affecting the development of food allergy

Re´sume´Les allergies alimentaires repre´sentent un sujet important pour la sante´. La pre´valence chez les adultes d'Europe Occidentale est estime´e entre 1 et 2 %, tandis que parmi les enfants elle est plus e´leve´e (autour de 5 %). La plupart des allergies confirme´es sont associe´es à une gamme de produits relativement re´duite, dont le lait de vache, les ufs, les noix, les cacahue´tes, le ble´, les poissons et les fruits de mer. Cependant, il est connu que la pre´valence des allergies aux aliments spe´cifiques varie selon la zone ge´ographique, en raison principalement des diffe´rences dans les habitudes alimentaires. Bien que nous n'en ayons pas la preuve formelle, on suppose que le nombre de cas d'allergie alimentaire, comme celui des autres formes de maladies atopiques, est en augmentation. Il n'y a aucun doute que la pre´disposition ge´ne´tique est un de´terminant important. Toutefois, il est connu que l'acquisition de la sensibilisation aux prote´ines alimentaires et par la suite au proble`me d'allergie est influence´e par un e´ventail de facteurs environnementaux, ainsi que par le moment, la dure´e et l'intensite´ de l'exposition. De plus, la nature de l'allerge`ne lui-meme peut avoir un rle tre`s important sur la se´ve´rite´ et la persistance de la maladie clinique. Notre objectif est ici de discuter de l'importance de quelques-unes de ces variables dans le contexte des re´ponses allergiques immunoglobuline E-dependant.
Defining functional domains within PF16: a central apparatus component required for flagellar motility.

Mutations affecting the assembly and stability of the central apparatus result in flagellar paralysis. Chlamydomonas cells with mutations at the PF16 locus have paralyzed flagella, and the C1 microtubule of the central apparatus is missing in isolated axonemes. On the basis of its mutant phenotype, sequence, and localization, PF16, a member of the armadillo repeat containing family of proteins, is involved in protein-protein interactions required for stability of the C1 microtubule and flagellar motility. Previous biochemical analysis of flagella isolated from pf16 cells demonstrated that assembly of the PF16 protein is either dependent on, or required for, the assembly of at least two other flagellar components. As a first step toward identifying functional domains in the PF16 protein that are essential for these interactions, we have characterized three mutations at the PF16 locus. In addition, we have generated deletion constructs of the PF16 gene and tested for their ability to assemble and rescue motility upon transformation of mutant pf16 cells. Our results demonstrate that the first armadillo repeat is necessary but not sufficient for assembly; that the C-122 amino acids are not required for assembly or motility; and that the repeats appear to form a single functional unit required for PF16 assembly.
Temporal firing characteristics and the strategic role of subicular neurons in short‐term memory

The role of subicular neurons is explored with respect to their participation in short‐term memory during performance of a spatial Delayed‐Nonmatch‐to‐Sample (DNMS) task by well‐trained rats. Subicular and CA1 neuron firing was examined in the same animals in relation to the encoding of task‐relevant events during the DNMS trial. The results indicate that subicular neurons have completely different firing signatures than well‐characterized hippocampal neurons in this task. Firing patterns of subicular neurons consisted of five different categories spanning all three phases of the DNMS trial, but concentrated mostly within the Sample and early portion of the Delay period. Unlike hippocampal neurons, subicular cells did not exhibit conjunctive firing correlates with respect to particular combinations of task events; rather, subicular cell firing was differentiated primarily on the basis of temporal specificity within the trial. Only two of the five subicular cell types fired differentially on correct versus error trials; however, one cell type exhibited such differential firing as an inverse function of duration of delay interval. Experiments employing γ‐aminobutyric acid GABAB receptor agonists and antagonists showed that both behavioral performance as well as subicular cell firing were disrupted significantly by baclofen at short delays, while performance at long delays and hippocampal cell firing were relatively immune to this effect. The relevance of subicular cell firing in the task with respect to its temporal relation to delay‐dependent hippocampal neuronal activity suggests that the structures have complementary roles in the encoding and representation of items in short‐term memory. Hippocampus 2003;13:529–541. © 2003 Wiley‐Liss, Inc.
Cancer statistics for African Americans, 2013

In this article, the American Cancer Society estimates the number of new cancer cases and deaths for African Americans and compiles the most recent data on cancer incidence, mortality, survival, and screening prevalence based upon incidence data from the National Cancer Institute, the Centers for Disease Control and Prevention, and the North American Association of Central Cancer Registries and mortality data from the National Center for Health Statistics. It is estimated that 176,620 new cases of cancer and 64,880 deaths will occur among African Americans in 2013. From 2000 to 2009, the overall cancer death rate among males declined faster among African Americans than whites (2.4% vs 1.7% per year), but among females, the rate of decline was similar (1.5% vs 1.4% per year, respectively). The decrease in cancer death rates among African American males was the largest of any racial or ethnic group. The reduction in overall cancer death rates since 1990 in men and 1991 in women translates to the avoidance of nearly 200,000 deaths from cancer among African Americans. Five‐year relative survival is lower for African Americans than whites for most cancers at each stage of diagnosis. The extent to which these disparities reflect unequal access to health care versus other factors remains an active area of research. Overall, progress in reducing cancer death rates has been made, although more can and should be done to accelerate this progress through ensuring equitable access to cancer prevention, early detection, and state‐of‐the‐art treatments. CA Cancer J Clin 2013. © 2013 American Cancer Society.
Development and differentiation of glial precursor cells in the rat cerebellum

The development and differentiation of bipotential glial precursor cells has been studied extensively in tissue culture, but little is known about the distribution and fate of these cells within intact animals. To analyze the development of glial progenitor cells in the developing rat cerebellum, we utilized immunofluorescent, immunocytochemical, and autoradiographic techniques. Glial progenitor cells were identified with antibodies against the NG2 chondroitin‐sulfate proteoglycan, a cell‐surface antigen of 02A progenitor cells in vitro, and the distribution of this marker antigen was compared to that of marker antigens that identify immature astrocytes, mature astrocytes, oligodendrocyte precursors, and mature oligodendrocytes.
BARRIERS TO UPTAKE OF BREAST CANCER SCREENING IN KENYA.

OBJECTIVES To conduct clinical breast cancer screening in three sites in Western Kenya and explore community barriers to screening uptake. DESIGN Cross-sectional study. SETTING Western Kenya specifically, Mosoriot, Turbo, and Kapsokwony. SUBJECTS Community members (18 years and older) who did not attend the screening events. OUTCOME MEASURE The outcome measure was having heard about the breast cancer screening events. Both structured and open-ended questions were used for data collection. Item frequency, correlations, and content analyses were performed. RESULTS A total of 733 community members were surveyed (63% women, median age 33 years, IQR = 26-43). More than half (55%) of respondents had heard about the screening but did not attend. The majority of those who had heard about this particular screening had knowledge of screening availability in general (45% vs. 25%, p < 0.001). Only 8.0% of those who heard and 6.0% of those who had not heard of the screening event had previously undergone clinical breast exam (p = 0.20). Reasons for not attending the screening event were personal factors, including busy schedule (41.0%), perceived low personal risk (12.7%), lack of transport (4.2%), as well as health facility factors such as poor publicity (14.4%) and long queues (8.7%). CONCLUSION Barriers to breast cancer screening uptake were associated with inadequate publicity, perceived long waits at event and busy lives among community women.