Miracle-dz/UniFAR-FTUs huggingface dataset:
Phosholipase C-Related Inactive Protein Is Involved in Trafficking of γ2 Subunit-Containing GABAA Receptors to the Cell Surface

The subunit composition of GABAA receptors is known to be associated with distinct physiological and pharmacological properties. Previous studies that used phospholipase C-related inactive protein type 1 knock-out (PRIP-1 KO) mice revealed that PRIP-1 is involved in the assembly and/or the trafficking of γ2 subunit-containing GABAA receptors. There are two PRIP genes in mammals; thus the roles of PRIP-1 might be compensated partly by those of PRIP-2 in PRIP-1 KO mice. Here we used PRIP-1 and PRIP-2 double knock-out (PRIP-DKO) mice and examined the roles for PRIP in regulating the trafficking of GABAA receptors. Consistent with previous results, sensitivity to diazepam was reduced in electrophysiological and behavioral analyses of PRIP-DKO mice, suggesting an alteration of γ2 subunit-containing GABAA receptors. The surface numbers of diazepam binding sites (α/γ2 subunits) assessed by [3H]flumazenil binding were reduced in the PRIP-DKO mice as compared with those of wild-type mice, whereas the cell surface GABA binding sites (α/β subunits, assessed by [3H]muscimol binding) were increased in PRIP-DKO mice. The association between GABAA receptors and GABAA receptor-associated protein (GABARAP) was reduced significantly in PRIP-DKO neurons. Disruption of the direct interaction between PRIP and GABAA receptor β subunits via the use of a peptide corresponding to the PRIP-1 binding site reduced the cell surface expression of γ2 subunit-containing GABAA receptors in cultured cell lines and neurons. These results suggest that PRIP is implicated in the trafficking of γ2 subunit-containing GABAA receptors to the cell surface, probably by acting as a bridging molecule between GABARAP and the receptors.
Genetic analysis of self-reported physical activity and adiposity: The Southwest Ohio Family Study

Abstract Objective Physical inactivity poses a major risk for obesity and chronic disease, and is influenced by both genetic and environmental factors. However, the genetic association between physical activity (PA) level and obesity is not well characterized. Our aims were to: (i) estimate the extent of additive genetic influences on physical activity while adjusting for household effects; and (ii) determine whether physical activity and adiposity measures share common genetic effects. Subjects The sample included 521 (42 % male) adult relatives, 18–86 years of age, from five large families in the Southwest Ohio Family Study. Design Sport, leisure and work PA were self-reported (Baecke Questionnaire of Habitual Physical Activity). Total body and trunk adiposity, including percentage body fat (%BF), were measured using dual-energy X-ray absorptiometry. Abdominal visceral and subcutaneous adipose tissue mass were measured using MRI. Results Heritabilities for adiposity and PA traits, and the genetic, household and environmental correlations among them, were estimated using maximum likelihood variance components methods. Significant genetic effects (P < 0·05) were found for sport (h2 = 0·26) and leisure PA (h2 = 0·17). Significant (P < 0·05) household effects existed for leisure PA (c2 = 0·25). Sport PA had a negative genetic correlation with central adiposity measurements adjusted for height (ρG > |−0·40|). Sport and leisure PA had negative genetic correlations with %BF (ρG > |−0·46|). Conclusions The results suggest that the association of sport and leisure PA with lower adiposity is due, in part, to a common genetic inheritance of both reduced adiposity and the predisposition to engage in more physical activity.
Prevalence and factors associated with dental caries among children and adults in selected districts in Uganda.

OBJECTIVE The aim was to determine the prevalence and factors associated with dental caries among adults and children in seven districts of Uganda. METHODS Participants aged 11-13 (n=1230) and 35-44 years (n=648) were randomly selected from urban and rural areas of Gulu, Soroti, Jinja, Masaka, Kabale, Kabarole and Hoima districts. They were examined by 4 trained and calibrated dentists for dental caries using Decayed, Missing and Filled teeth index as described by World Health Organisation. RESULTS Overall mean DMFT score was 0.73 for children and 4.71 for adults. Generally, there was a higher mean DMFT score in the rural (2.19) compared to urban areas (1.97). In all the districts, except Hoima, there was a higher mean DMFT score of children in rural compared to urban. In adults, similar trend was mainly registered in Masaka, Hoima and Gulu districts. Most participants (79.9%, n=1309) occasionally ate sugared snacks. Overall, 95% (n=1795) of the participants cleaned their teeth with plastic tooth brushes (71.7%) and chewing sticks (8.3%). CONCLUSION Although the severity of dental caries was low, the disease was widespread in the study population. A high proportion of participants reported consumption of sugared snacks and drinks, which calls for oral health education.
A quantitative evaluation of apoptotic bodies in rat liver.

The aim of the present study was to determine if data on the number and acinar distribution of apoptotic bodies (AB) in normal liver could help in the understanding of cell kinetics in the liver, and the mechanism of early ethanol-induced liver damage. Normal male Sprague-Dawley rats were studied. They had free access to Purina chow and drinking water. Ethanol-treated rats received the drug at increasing concentration in drinking water for 5 weeks. The following parameters were measured: number of AB in the lobule, topographical localization, distance from terminal hepatic veins (THV), i.e. row of hepatocytes concerned, H1 being the closest to the THV. The results show that AB are rare in the normal liver and are always observed in zone 3, next to the THV. Of 149 THV examined, 56 showed one associated AB, exceptionally two. 74% of the AB were confined to the first row of hepatocytes (H1), 21% to H2, 4% to H3, and 1% to H4. In ethanol-treated rats the mean number of AB was 2 or 3 for each THV. 42% were found in H1, 32% in H2, 15% in H3, 7% in H4, and 4% in H5. The data show that AB are not randomly dispersed in normal liver but show a preferential acinar distribution. In addition, most AB are located in the row of liver cells immediately adjacent to the THV. If apoptosis is indeed an expression of physiological cell renewal, these findings support the concept that zone 3 contains older hepatocytes. (ABSTRACT TRUNCATED AT 250 WORDS)
Repellency of DEET, Picaridin, and Three Essential Oils to Triatoma rubida (Hemiptera: Reduviidae: Triatominae)

ABSTRACT The kissing bug, Triatoma rubida (Uhler) is a common hematophagous bug in Tucson, AZ, and is responsible for causing severe allergic reactions in some bitten individuals. DEET, picaridin, tea tree oil, peppermint oil, and citronella oil were tested for repellency to T. rubida and its ability to probe and feed on a small restrained rat. No long range repellency was observed with any of the test materials. The lowest repellent concentrations observed were: 10% DEET, 7% picaridin; 30% tea tree oil, 3.3% peppermint oil, and 0.165% citronella oil. Only citronella oil was able to stop all probing and feeding by T. rubida. Citronella oil appears to be a promising potential repellent to prevent sleeping people from being bitten by kissing bugs.
Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits

Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g. 407_408 insC in the DGAT1 gene, AJ237937:g.6798C>T and g.6852C>T in the STAT5A gene. In DGAT1 g. 407_408insC locus, the frequencies of C - allele were 0.79 - 0.85, and frequencies of C + allele were 0.21 - 0.15. At STAT5A g.6852C>T locus, frequencies of C allele were 0.70 - 0.72, and frequencies of T allele were 0.30 - 0.28. Compared with goats with DGAT1 C - C - , those with C - C + genotype had greater milk fat ( P < 0.05). The goats with STAT5A CT had greater milk yield than those with CC genotype ( P < 0.05). The results showed that does with C - C - CT and C - C + CT yielded more milk than those with C - C - CC (P < 0.05). In addition, does with C - C + CT had the highest milk fat in comparison with other combination genotypes ( P < 0.05).
Morality, Ethics, and Reflection: A Categorization of Normative IS Research

Moral views and perceptions, their ethical evaluation and justification, and practical concerns about how to incorporate them all play important roles in research and practice in the information systems discipline. This paper develops a model of normative issues ranging from moral intuition and explicit morality to ethical theory and meta-ethical reflection. After showing that this normative model is relevant to IS and that it allows an improved understanding of normative issues, the paper discusses these levels of normativity in the context of two of the most prominent normative topics in IS: Privacy and intellectual property. The paper then suggests that a more explicit understanding of the different aspects of normativity would benefit IS research. This would leverage the traditional empirical strengths of IS research and use them to develop research that is relevant beyond the boundaries of the discipline. Such broader relevance could be aimed at the reference disciplines. In particular, moral philosophy could benefit from understanding information technology and its role in organizations in more detail. It could, furthermore, inform policy makers who are increasingly called on to regulate new information technologies.
Probiotics and the Prevention of Necrotizing Enterocolitis

matory response of the immature intestinal immune system, resulting in rapidly evolving intestinal gangrene, perforation, sepsis, shock and death [1, 2, 4] . Probiotics have been shown to be able to modify both the intestinal microbiota as well as the intestinal immune response. On this background, several clinical trials have been carried out in order to investigate the potential role of probiotics in the prevention of necrotizing enterocolitis [5–13] . Most of these studies, among them many randomized controlled trials, suggest a significant benefit of probiotics in the prevention of necrotizing enterocolitis, confirming previous observations in experimental animal models [14, 15] . Possible mechanisms of probiotics in the prevention of necrotizing enterocolitis are beneficial modification of the intestinal microbiota, strengthening of the intestinal mucosal barrier and attenuating the intestinal immune response to bacteria and bacterial products. The results of the randomized controlled trials have been analyzed in several comments and reviews [16–19] . There is a general agreement that probiotic supplementation has a great potential to significantly reduce the risk of necrotizing enterocolitis and the overall mortality in premature infants with very low birth weight. However, there is obviously great variability of the clinical trials reported, with respect to inclusion criteria of the patients, Necrotizing enterocolitis is one of the most serious gastrointestinal emergencies in the neonatal intensivecare unit [1] . It predominantly affects preterm infants. The onset of disease is usually between the 3rd and the 10th day of life. Necrotizing enterocolitis presents with a wide spectrum of clinical symptoms, ranging from nonspecific gastrointestinal disturbance to a fulminant course of intestinal gangrene and perforation. Typical early signs are abdominal distension, delayed gastric emptying, diarrhea, abdominal tenderness and gastrointestinal bleeding. Characteristically, extensive hemorrhagic inflammatory necrosis of the terminal ileum and ascending colon can be found. In severe cases the entire bowel may be involved. Intramural hemorrhage, gangrene, peritonitis and edema may progress to intestinal necrosis with extensive infiltration of neutrophil leukocytes. Risk factors include prematurity, hypoxia, formula feeding, bacterial infection and intestinal ischemia [2, 3] . Mortality is high (15–30%) and related to the presence of bacteremia, disseminated intravascular coagulation, ascites and very low birth weight. Etiology and pathogenesis of necrotizing enterocolitis remain unknown. However, formula feeding, intestinal mucosal barrier dysfunction and dysbalance of bacterial colonization may be contributing factors, leading to an inappropriate inflam-
Noncontact Accurate Measurement of Cardiopulmonary Activity Using a Compact Quadrature Doppler Radar Sensor

The designed sensor enables accurate reconstruction of chest-wall movement caused by cardiopulmonary activities, and the algorithm enables estimation of respiration, heartbeat rate, and some indicators of heart rate variability (HRV). In particular, quadrature receiver and arctangent demodulation with calibration are introduced for high linearity representation of chest displacement; 24-bit ADCs with oversampling are adopted for radar baseband acquisition to achieve a high signal resolution; continuous-wavelet filter and ensemble empirical mode decomposition (EEMD) based algorithm are applied for cardio/pulmonary signal recovery and separation so that accurate beat-to-beat interval can be acquired in time domain for HRV analysis. In addition, the wireless sensor is realized and integrated on a printed circuit board compactly. The developed sensor system is successfully tested on both simulated target and human subjects. In simulated target experiments, the baseband signal-to-noise ratio (SNR) is 73.27 dB, high enough for heartbeat detection. The demodulated signal has 0.35% mean squared error, indicating high demodulation linearity. In human subject experiments, the relative error of extracted beat-to-beat intervals ranges from 2.53% to 4.83% compared with electrocardiography (ECG) R-R peak intervals. The sensor provides an accurate analysis for heart rate with the accuracy of 100% for p = 2% and higher than 97% for p = 1%.
Perception and Attitudes of Firefighters on Noise Exposure and Hearing Loss

Noise exposure of firefighters results in increased risk of hearing loss. Noise-induced hearing loss (NIHL) can be prevented by the consistent use of hearing protection devices (HPDs). However, little is known about firefighters' perceptions and attitudes on NIHL and HPD use. The purposes of the study were (1) to identify common noise sources of fire fighting equipment and activities; and (2) to describe the attitudes and beliefs of firefighters concerning the importance of their hearing, occupational noise exposure, NIHL, and HPD use. A nonexperimental, descriptive focus group design was used to gather qualitative data from firefighters at two fire houses in southeast Michigan. The study identified sources of noise unique to fire fighting, such as driving fire/emergency trucks with sirens on; operating water pumps, saws, and other equipment; and shouting to communicate with civilians and fellow firefighters. Firefighters acknowledged the significance of good hearing in fire fighting service but perceived NIHL was unavoidable on the job and viewed it as a small risk compared with other hazards. HPDs were not used regularly because firefighters believed they interfered with the ability to hear commands during emergency conditions, interfered with other required safety equipment, and were generally forgotten when gearing up. Firefighters recognized the importance of hearing health and the benefit of a hearing conservation program, including annual training, and identified several topics that should be included in a training program. These findings will provide critical information in planning what type of program should be developed and carried out for the fire fighting population.
Relations among maternal socialization, effortful control, and maladjustment in early childhood

Abstract In a sample of 18-, 30-, and 42-month-olds, the relations among parenting, effortful control (EC), and maladjustment were examined. Parenting was assessed with mothers' reports and observations; EC was measured with mothers' and caregivers' reports, as well as a behavioral task; and externalizing and internalizing symptoms were assessed with parents' and caregivers' reports. Although 18-month unsupportive (vs. supportive) parenting negatively predicted EC at 30 months, when the stability of these variables was taken into account, there was no evidence of additional potentially causal relations between these two constructs. Although EC was negatively related to both internalizing and externalizing problems within all three ages as well as across 1 year, EC did not predict maladjustment once the stability of the constructs and within time covariation between the constructs were taken into account. In addition, externalizing problems at 30 months negatively predicted EC at 42 months, and internalizing problems at 30 months positively predicted EC at 42 months, but only when the effects of externalizing on EC were controlled. The findings are discussed in terms of the reasons for the lack of causal relations over time.
DETECTION OF BLASTOCYSTIS HOMINIS IN UNPRESERVED STOOL SPECIMENS BY USING POLYMERASE CHAIN REACTION

Blastocystis hominis is a common enteric parasite of worldwide distribution. Its pathogenetic potential has not yet been established, although numerous case reports suggest that B. hominis may cause the development of various gastrointestinal symptoms and disorders. The detection of the parasite in stool specimens is conventionally done by microscopy of direct smears, fecal concentrates, or permanently stained smears; however, morphology-based diagnosis is problematic. The aim of this study was to develop and evaluate a polymerase chain reaction (PCR) technique for the direct detection of B. hominis in human stool samples. Primers were based on small subunit ribosomal DNA and able to detect ≥32 parasites/200 mg stool artificially spiked with cultured B. hominis. In the evaluation of 43 clinical specimens, the PCR was tested against the formol ethyl acetate concentration technique (FECT) and a culture technique, proving 100% test specificity and a significantly higher sensitivity than the FECT. The PCR method is recommended for screening clinical specimens for B. hominis infection and for use in prevalence studies.
CXCL12 (SDF‐1) and CXCL13 (BCA‐1) chemokines significantly induce proliferation and collagen type I expression in osteoblasts from osteoarthritis patients

To evaluate the role of CXC chemokines CXCL8 (IL8), CXCL10 (IP‐10), CXCL12 (SDF‐1), and CXCL13 (BCA‐1) in bone remodeling, we analyzed their effects on osteoblasts (OBs) obtained from subchondral trabecular bone tissue of osteoarthritis (OA) and post‐traumatic (PT) patients. The expression of CXC receptors/ligands (CXCR1/CXCL8, CXCR2/CXCL8, CXCR3/CXCL10, CXCR4/CXCL12, and CXCR5/CXCL13) was analyzed in cultured OBs by flow cytometry and immunocytochemistry. Functional assays on CXC chemokine‐treated‐OBs in the presence or absence of their specific inhibitors were performed to analyze cellular proliferation and the enzymatic response to chemokine activation. The expression of chemokine ligands/receptors was also confirmed in bone tissue samples by immunohistochemical analysis. Collagen type I and alkaline phosphatase mRNA expression were analyzed on CXCL12‐ and CXCL13‐treated OBs by real‐time PCR. OBs from both OA and PT patients expressed high levels of CXCR3 and CXCR5 and lower amounts of CXCR1 and CXCR4. CXCL12 and CXCL13, only in OBs from OA patients, induced a significant proliferation that was also confirmed by specific blocking experiments. Moreover, OBs from OA patients released a higher amount of CXCL13 than those of PT patients while no differences were found for CXCL12. In the remodeling area of bone tissue samples, immunohistochemical analysis confirmed that OBs expressed CXCL12/CXCR4 and CXCL13/CXCR5 both in OA and PT samples. CXCL12 and CXCL13 upregulated collagen type I mRNA expression in OBs from OA patients. These data suggest that CXCL12 and CXCL13 may directly modulate cellular proliferation and collagen type I in OA patients, so contributing to the remodeling process that occurs in the evolution of this disease. © 2005 Wiley‐Liss, Inc.
Correlation between myofibrillar ATPase activity and myosin heavy chain composition in equine skeletal muscle and the influence of training

The histochemical myofibrillar ATPase (mATPase) method is used routinely for identification of equine skeletal muscle fiber types, but important problems have been observed with the subdivision of fast fiber population when using this method. To verify the use of this qualitative method, a number of equine muscle biopsies were analyzed with a combination of histochemical, immunohistochemical, electrophoretic, and morphometric techniques. The influence of training on these interrelations was also evaluated.
Transgenerational stress memory in Arabidopsis thaliana (L.) Heynh.: antioxidative enzymes and HSP70

Transgenerational transmission of information about stress exposure is manifested as an increase in the somatic homologous recombination frequency in plants. Our aim was to investigate whether information about changes of antioxidative enzyme activities and protein HSP70 induction are also transmitted in response to stress caused by UV-C irradiation. These stress indicators were investigated in Arabidopsis thaliana plants exposed to UV-C irradiation (6 and 600 J m-2) and its non-irradiated progeny. The activity of catalase was significantly decreased in the irradiated plants in comparison to the non-irradiated control plants, while the activity of guaiacol peroxidase was increased. The ascorbate peroxidase activity was not significantly changed. In irradiated plants there was an induction of a new HSP70 protein isoform. In the non- irradiated progeny of irradiated plants, a significant decrease in catalase and ascorbate peroxidase activity was noticed in comparison to plants whose parents were not irradiated. There was no significant change in guaiacol peroxidase activity or induction of HSP70 isoforms in the progeny. The obtained results indicate that, besides the already known increase in frequency of somatic homologous recombination, transmission of information about stress exposure can also include changes in activities of antioxidative enzymes catalase and ascorbate peroxidase. The explanations for the observed changes and the mechanism by which they occur have to be established in further research.