Miracle-dz/UniFAR-FTUs huggingface dataset:
Impairment of adenylyl cyclase and of spatial memory function after microsphere embolism in rats

The purpose of the present study was to characterize alterations in the adenylyl cyclase (AC), cyclic adenosine 3′,5′‐monophosphate (cAMP), and spatial memory function after sustained cerebral ischemia. Sustained cerebral ischemia was induced by injection of 900 microspheres (48 μm in diameter) into the right (ipsilateral) hemisphere of rats. Alterations in the AC and cAMP in the cerebral cortex and hippocampus were examined up to 7 days after the embolism. A decrease in the cAMP content was seen in the ipsilateral hemisphere throughout the experiment. Microsphere embolism (ME) decreased the activity of Ca2+/calmodulin (CaM)‐sensitive AC in the ipsilateral hemisphere throughout the experiment, whereas the basal and 5′‐guanylyl imidodiphosphate (Gpp(NH)p)‐sensitive AC activities were not altered. Immunoblotting analysis of AC subtypes with specific antibodies showed a decrease in the immunoreactivity of AC‐I in the ipsilateral hemisphere during these periods. No significant differences in the immunoreactivity of AC‐V/VI and AC‐VIII were observed after ME. The levels of GTP‐binding proteins Gαs, Gαi, and Gβwere unchanged. Furthermore, microsphere‐embolized rats showed prolongation of the escape latency in the water maze task determined on the seveth to ninth day after the operation. These results suggest that sustained cerebral ischemia may induce the impairment of the AC, particularly a selective reduction in the AC‐I level and activity, coupled with the decrease in cAMP content. This reduction may play an appreciable role in the disturbance in cAMP‐mediated signal transduction system, possibly leading to learning and memory dysfunction. © 2002 Wiley‐Liss, Inc.
Invalid Measurement of Plasma Albumin Using Bromcresol Green Methodology in Penguins (Spheniscus species)

Abstract The purpose of this study was to examine the validity of albumin determinations in penguin plasma by the bromcresol green (BCG) method and the gold standard of protein electrophoresis (EPH). Plasma from 96 clinically normal and abnormal penguins (Spheniscus species) was analyzed. The 2 methods did not yield equivalent results. The BCG method underestimated the albumin level in samples from normal patients (indicated by a normal albumin ∶ globulin ratio) and overestimated the albumin level in samples from clinically abnormal penguins (indicated by a decreased albumin ∶ globulin ratio). After EPH of plasma samples from clinically abnormal penguins samples was performed to separate albumin and globulin fractions, the globulins exhibited marked binding to the BCG dye. There were no significant differences between the variable reaction of paired serum and plasma samples when using the BCG method. These results demonstrated marked differences in the determination of albumin levels when using the BCG method and protein EPH. They further demonstrated that the BCG method can provide erroneous results, which have the potential to significantly impact clinical diagnosis and treatment. This study confirmed findings from previous studies in other avian species that the BCG method yields unreliable results in avian species. It is our conclusion that the BCG method, commonly found on automated analyzers in commercial laboratories and on point-of-care analyzers, should not be used to determine albumin concentration in avian samples.
Bayesian algorithm for microwave-based precipitation retrieval: description and application to TMI measurements over ocean

A physically oriented inversion algorithm to retrieve precipitation from satellite-based passive microwave measurements named the Bayesian algorithm for microwave-based precipitation retrieval (BAMPR) is proposed. First, we illustrate the procedure that BAMPR follows to produce precipitation estimates from observed multichannel brightness temperatures. Retrieval products are the surface rain rates, columnar equivalent water contents, and hydrometeor content profiles, together with the associated estimation uncertainties. Numerical tests performed on simulated measurements show that retrieval errors are reduced when a rain type and pattern classification procedure is employed, and that estimates are quite sensitive to the adopted error model. Finally, for different tropical storms that were observed by the Tropical Rainfall Measuring Mission (TRMM) Microwave Imager (TMI), we compare the rain retrieved from BAMPR relative to those retrieved from the Goddard Profiling (Gprof) algorithm and the Precipitation Radar-adjusted TMI estimation of rainfall (PATER) algorithm. Despite a similar inversion approach, the algorithms exhibit different performances that can be mainly related to different training databases and retrieval constraints such as cloud classification.
A Protein Kinase C Translocation Inhibitor as an Isozyme-selective Antagonist of Cardiac Function*

Protein kinase C (PKC) isozymes translocate to unique subcellular sites following activation. We previously suggested that translocation of activated isozymes is required for their function and that in addition to binding to lipids, translocation involves binding of the activated isozymes to specific anchoring proteins (receptors for activated protein kinase C. Using cultured cardiomyocytes we identified inhibitors, the V1 fragment of εPKC (εV1), and an 8-amino acid peptide derived from it that selectively inhibited the translocation of εPKC. Inhibition of εPKC translocation but not inhibition of δ or βPKC translocation specifically blocked phorbol ester- or norepinephrine-mediated regulation of contraction. These isozyme-selective translocation inhibitors provide novel tools to determine the function of individual PKC isozymes in intact cells.
Foreign Body in the Tracheobronchial Tree

A 20-year experience with the treatment of 74 patients (83.8% children) for foreign body aspiration is reviewed. The object of this review is to show the clinical manifestations, the radiological findings, the nature and distribution in the bronchial tree, and complications due to longstanding (months or years) foreign bodies in the bronchial tree. The most common foreign bodies found were peanuts (13.5%), corn (13.5%), and beans (13.5%). The most frequent clinical manifestation was choking (67.5%), and the most frequent radiological finding was atelectasis (41.8%). The most serious complication was bronchiectasis needing resection in six patients who had the foreign body retained for years in the bronchial tree. In conclusion, in spite of an obvious foreign body in the tracheobronchial tree many cases are not diagnosed, and a longstanding foreign body in the airway may be responsible for irreversible complications.
Retinal microvasculature and white matter microstructure

Objective: To investigate whether retinal microvascular damage is related to normal-appearing white matter microstructure on diffusion tensor MRI. Methods: We included 2,436 participants (age ≥45 years) from the population-based Rotterdam Study (2005–2009) who had gradable retinal images and brain MRI scans. Retinal arteriolar and venular calibers were measured semiautomatically on fundus photographs. White matter microstructure was assessed using diffusion tensor MRI. We used linear regression models to investigate the associations of retinal vascular calibers with markers of normal-appearing white matter microstructure, adjusting for age, sex, the fellow vascular caliber, and additionally for structural MRI markers and cardiovascular risk factors. Results: Narrower arterioles and wider venules were associated with poor white matter microstructure: adjusted difference in fractional anisotropy per SD decrease in arteriolar caliber −0.061 (95% confidence interval −0.106 to −0.016), increase in venular caliber −0.054 (−0.096 to −0.011), adjusted difference in mean diffusivity per SD decrease in arteriolar caliber 0.048 (0.007–0.088), and increase in venular caliber 0.047 (0.008–0.085). The associations for venules were more prominent in women. Conclusions: Retinal vascular calibers are related to normal-appearing white matter microstructure. This suggests that microvascular damage in the white matter is more widespread than visually detectable as white matter lesions.
Regularized Optimal Transport and the Rot Mover's Distance

This paper presents a unified framework for smooth convex regularization of discrete optimal transport problems. In this context, the regularized optimal transport turns out to be equivalent to a matrix nearness problem with respect to Bregman divergences. Our framework thus naturally generalizes a previously proposed regularization based on the Boltzmann-Shannon entropy related to the Kullback-Leibler divergence, and solved with the Sinkhorn-Knopp algorithm. We call the regularized optimal transport distance the rot mover's distance in reference to the classical earth mover's distance. We develop two generic schemes that we respectively call the alternate scaling algorithm and the non-negative alternate scaling algorithm, to compute efficiently the regularized optimal plans depending on whether the domain of the regularizer lies within the non-negative orthant or not. These schemes are based on Dykstra's algorithm with alternate Bregman projections, and further exploit the Newton-Raphson method when applied to separable divergences. We enhance the separable case with a sparse extension to deal with high data dimensions. We also instantiate our proposed framework and discuss the inherent specificities for well-known regularizers and statistical divergences in the machine learning and information geometry communities. Finally, we demonstrate the merits of our methods with experiments using synthetic data to illustrate the effect of different regularizers and penalties on the solutions, as well as real-world data for a pattern recognition application to audio scene classification.
Lack of relationship between obesity and mortality or morbidity after coronary artery bypass grafting.

AIMS Obesity has significant adverse effects on cardiovascular health. Conflicting results have been reported regarding relationships between body mass index (BMI) and outcomes after coronary artery bypass grafting (CABG). We compared outcomes of CABG patients by BMI categories. METHODS Isolated CABG performed between July 2010-June 2012 at Auckland City Hospital were categorised into four groups of BMI ≤25 (normal), >25-30 (overweight), >30-35 (obese) and >35 kg/m² (morbidly obese) retrospectively for analyses. RESULTS The four groups had 181(22.4%), 320 (39.6%), 205 (25.3%) and 103 (12.7%) patients respectively. Increasing BMI was associated with younger age (p<0.001) and increasing creatinine clearance (p<0.001). Obesity was associated with a higher proportion of patients of Maori or Pacific ethnicity and patients with more hypertension. Morbid obesity was associated with female sex, higher mean New Zealand Deprivation Index, diabetes, longer operation time and sternal wound infection. Thirty-day mortality (p=0.702), composite morbidity (p=0.904) and survival (p=0.112) during 1.4 ± 0.6 years of follow-up were similar across BMI categories. CONCLUSION Obesity was common and was present in over a third of patients undergoing CABG with 13% of the entire cohort being morbidly obese. Mortality and morbidity rates did not differ across BMI categories. Obesity should not be considered a risk factor for adverse outcomes after CABG and should not be a contraindication for surgery.
Outcome of progressive disease after autologous stem cell transplantation in patients with non‐Hodgkin's lymphoma: a nation‐wide survey

Abstract: Objectives: To analyse outcome and prognostic factors in non‐Hodgkin's lymphoma (NHL) patients who progress after autologous stem cell transplantation (ASCT). Patients: Altogether 115 consecutive NHL patients transplanted in 1991–2000 were studied. Histology included diffuse large B cell (n = 52), follicular (n = 26), mantle cell (n = 15), T cell (n = 16) and other subtypes (n = 6). The median time from ASCT to the progression was 7 months. Ninety‐six patients (83%) received salvage treatment. Results: Twenty‐four patients (25%) achieved complete remission and 30 (31%) partial remission. The median overall survival was 8 months (range 0–98+) and the projected 4‐year survival 21%. In multivariate analysis factors predicting treatment response after the progression included the use of rituximab (P = 0.036), histology other than diffuse large B cell (P = 0.001) and International Prognostic Index ≤2 at progression (P < 0.001). Normal lactate dehydrogenase (LDH) at progression (P = 0.002), response to salvage treatment (P < 0.001) and time from ASCT to progression ≥7 months (P = 0.022) were predictors for overall survival. Conclusions: Although the prognosis of patients who progress after ASCT is generally poor, many patients will respond to current therapies, and some may experience prolonged survival. Normal LDH at time of disease progression and longer time to progression after ASCT were the most powerful predictors for a promising outcome.
Does restructuring hospitals result in greater efficiency - an empirical test using diachronic data

Hospitals are being restructured more frequently. Increased cost efficiency is the usual justification given for such changes. All 20 major teaching hospitals in Australia's two most populous states were investigated by classifying each over a 5-6 year period in terms of their cost efficiency (average cost per case weighted by Australian diagnosis-related group [AN-DRG] data and adjusted for inflation) and structure, categorized as traditional-professional (TP), clinical-divisional (CD), or clinical-institute (CI). In all, 12 hospitals changed structure during the study period. There was slight evidence that CD structures were more efficient than TP structures but this was not supported by other evidence. There were no significant differences in efficiency in the first or second years following changes from either TP to CD or TP to CI structures. All four hospitals changing from CD to CI structure became significantly less efficient. This may be due to frequency rather than type of change as they were the only hospitals that implemented two structural changes. Hospitals that changed or did not change structure were similar in efficiency at the beginning and at the end of the study period, in overall efficiency during the period, and in trends toward efficiency over time. The findings challenge those who advocate restructuring hospitals on the grounds of improving cost efficiency.
Human Vγ2Vδ2 T cells limit breast cancer growth by modulating cell survival‐, apoptosis‐related molecules and microenvironment in tumors

Innate immune system has been known to play an important role in inhibiting the malignant transformation, tumor progression and invasion. However, the mechanistic basis remains ambiguous. Despite polyclonality of human γδ T cells, Vγ2Vδ2 T cell subset was shown to recognize and limit the growth of various tumors at various degrees. The differential recognition of the tumor cells by Vγ2Vδ2 T cells are yet to be defined. Our study reveals that γδ T cells limit in vitro growth of most breast tumor cells, such as SkBr7 (HER2+), MCF7 (ER+) and MDA‐MB‐231 (ER−) by inhibiting their survival and inducing apoptosis, except BrCa‐MZ01 (PR+) cells. To investigate detail mechanisms of antineoplastic effects, we found that cell death was associated with the surface expression levels of MICA/B and ICAM1. Molecular signaling analysis demonstrated that inhibition of cell growth by γδ T cells was associated with the lower expression levels of cell survival‐related molecules such as AKT, ERK and concomitant upregulation of apoptosis‐related molecules, such as PARP, cleaved caspase 3 and tumor suppressor genes PTEN and P53. However, opposite molecular signaling was observed in the resistant cell line after coculture with γδ T cells. In vivo, antineoplastic effects of γδ T cells were also documented, where tumor growth was inhibited due to the downregulation of survival signals, strong induction of apoptotic molecules, disruption of microvasculature and increased infiltration of tumor associated macrophages. These findings reveal that a complex molecular signaling is involved in γδ T cell‐mediated antineoplastic effects.
Ensemble modeling of denoising autoencoder for speech spectrum restoration

Denoising autoencoder (DAE) is effective in restoring clean speech from noisy observations. In addition, it is easy to be stacked to a deep denoising autoencoder (DDAE) architecture to further improve the performance. In most studies, it is supposed that the DAE or DDAE can learn any complex transform functions to approximate the transform relation between noisy and clean speech. However, for large variations of speech patterns and noisy environments, the learned model is lack of focus on local transformations. In this study, we propose an ensemble modeling of DAE to learn both the global and local transform functions. In the ensemble modeling, local transform functions are learned by several DAEs using data sets obtained from unsupervised data clustering and partition. The ﬁnal transform function used for speech restoration is a combination of all the learned local transform functions. Speech denoising experiments were carried out to examine the performance of the proposed method. Experimental results showed that the proposed ensemble DAE model provided superior restoration accuracy than traditional DAE models.
Two different first-line 5-fluorouracil regimens with or without oxaliplatin in patients with metastatic colorectal cancer.

BACKGROUND Oxaliplatin, 5-fluorouracil (5-FU), and leucovorin (LV) are standard first-line treatments for patients with metastatic colorectal cancer (mCRC). The aim of this multicentre, open-label, phase IIIb study was to assess the addition of oxaliplatin to two different 5-FU regimens. PATIENTS AND METHODS Patients with previously untreated mCRC were randomised to arm A [two-weekly oxaliplatin 85 mg/m(2) + either continuous intravenous infusion (CIV) of 5-FU without LV or two-weekly bolus and CIV 5-FU + LV (LV5FU2)] or arm B (5-FU CIV or LV5FU2 alone). Irinotecan monotherapy was planned on progression. RESULTS A total of 725 patients were enrolled. After a fixed follow-up of 2 years for each patient, 2-year survival rates were 27.3% and 24.8% in arms A and B, respectively (hazard ratio 0.93; 95% confidence interval 0.78-1.10). The addition of oxaliplatin significantly improved response rates (54.1 versus 29.8%; P < 0.0001) and median progression-free survival (7.9 versus 5.9 months; P < 0.0001). The most common grade 3-4 toxic effects were neutropenia (arm A, 33%; arm B, 5%), diarrhoea (arm A, 14%; arm B, 8%), and fatigue (arm A, 9%; arm B, 8%). CONCLUSIONS Despite improved rates of tumour control, these results failed to demonstrate a survival benefit from the addition of oxaliplatin to infused 5-FU and lend further support to the use of sequential monotherapy in some patients with mCRC.
Diagnostic accuracy of the care dependency scale.

AIM This paper reports an investigation of the diagnostic accuracy of the Care Dependency Scale (CDS). BACKGROUND Assessment tools can be described in terms of diagnostic accuracy, or the ability to correctly classify subjects into clinically relevant subgroups. Diagnostic accuracy can be determined by several techniques as sensitivity, specificity, receiver operating curve analysis and likelihood ratios. METHOD A cross-sectional design was used with data from 237 patients from two studies. Data were collected using a questionnaire consisting of the CDS and the Barthel Index (BI). The CDS is a relatively new instrument and should be validated by comparison against an established gold standard, in this case the BI. Measures to quantify the validity of diagnostic tests, such as sensitivity, specificity, positive and negative predictive values, prevalence and likelihood ratios were calculated. In addition, the receiver operating characteristics (ROC) curve analysis was used to report the test accuracy of the CDS and to determine an appropriate cut-off point for care dependency detection. FINDINGS The prevalence in the sample study was very high (84%). The area under the ROC curve for the CDS was 0.81, which indicates moderate diagnostic accuracy. Patients with a CDS sumscore </=68 (rule-out cut-off point) were classified as care dependent, all others as independent. The determination of the appropriate cut-off point was based on sensitivity (0.85) and positive predictive valued (0.90). CONCLUSION The CDS may be used for to estimate care dependency among hospital patients with various conditions.
Baicalin reduces mitochondrial damage in streptozotocin‐induced diabetic Wistar rats

Hyperglycemia‐induced superoxide production in the mitochondria is known to be the primary cause of diabetic micro‐ and macro‐vascular complications and mitochondrial membranal damage. This study in streptozotocin‐induced diabetic Wistar rats investigated the anti‐hyperglycemic and mitochondrial membrane protection effects of baicalin, a flavonoid known for its radical scavenging activity.
Multiple-dose pharmacokinetics of ciprofloxacin administered intravenously to normal volunteers

We administered multiple doses of ciprofloxacin intravenously over 30 min every 12 h for 1 week to nine healthy volunteers. Three volunteers received a placebo (vehicle) intravenously. Doses of 100, 150, and 200 mg were evaluated with a 1-week wash-out period intervening between each dose level. Terminal excretion half-lives averaged 3.67 +/- 0.65, 3.60 +/- 0.26, and 4.00 +/- 0.69 h for the 100-, 150-, and 200-mg doses, respectively. Serum clearances were 30.1 +/- 3.4, 29.8 +/- 4.0, and 26.9 +/- 4.1 liters/h per 1.73 m2 for these doses. Urine concentrations remained in excess of the MIC for 90% of the relevant urinary tract pathogens for the full 12-h dosing interval at each dose. Renal clearance accounted for 56 to 71% of the serum clearance. However, because a microbiologic assay was used and biologically active, renally excreted metabolites were identified, the renal clearance determinations are likely to be in excess of the true values. The doses of ciprofloxacin administered intravenously were well tolerated, and the drug concentrations appeared adequate for the treatment of the vast majority of cases of nosocomially acquired sepsis and urinary tract infections. For patients with serious Pseudomonas infections and perhaps staphylococcal infections, either an 8-h dosing schedule or larger doses on a 12-h schedule should be considered.