Miracle-dz/UniFAR-FTUs huggingface dataset:
Role in Cell Permeability of an Essential Two-Component System in Staphylococcus aureus

ABSTRACT A temperature-sensitive lethal mutant of Staphylococcus aureus was found to harbor a mutation in the uncharacterized two-component histidine kinase (HK)-response regulator (RR) pair encoded by yycFG; orthologues of yycFG could be identified in the genomes of Bacillus subtilis and other gram-positive bacteria. Sequence analysis of the mutant revealed a point mutation resulting in a nonconservative change (Glu to Lys) in the regulator domain of the RR at position 63. To confirm that this signal transduction system was essential, a disrupted copy of either the RR (yycF) or the HK (yycG) was constructed with a set of suicide vectors and used to generate tandem duplications in the chromosome. Resolution of the duplications, leaving an insertion in either the yycF or the yycG coding region, was achieved only in the presence of an additional wild-type copy of the two open reading frames. Phenotypic characterization of the conditional lethal mutant showed that at permissive growth conditions, the mutant was hypersusceptible to macrolide and lincosamide antibiotics, even in the presence of the ermB resistance determinant. Other mutant phenotypes, including hypersensitivity to unsaturated long-chain fatty acids and suppression of the conditional lethal phenotype by high sucrose and NaCl concentrations, suggest that the role of the two-component system includes the proper regulation of bacterial cell wall or membrane composition. The effects of this point mutation are strongly bactericidal at the nonpermissive temperature, indicating that this pathway provides an excellent target for the identification of novel antibiotics.
Spirometry utilization in Ontario: practice patterns and policy implications.

OBJECTIVE To describe growth and regional variation in the use of spirometry (flow studies) in Ontario. DESIGN Retrospective analysis of Ontario Health Insurance Plan (OHIP) fee-for-service billing data for spirometry from the 1989-90 to 1994-95 fiscal years. SETTING Physicians' office practices in Ontario. OUTCOME MEASURES Number of flow studies and associated expenditures, number and specialty of physicians performing flow studies and the distribution of their billings, number of studies per capita by age group of patients, expenditures by region and measures of variation among regions. RESULTS In 1994-95, $14.13 million was spent on flow studies in Ontario. This expenditure increased by 36.9% from 1989-90 to 1994-95, exceeding the overall growth rate of 20.8% for all expenditures under OHIP. Expenditure growth was driven by an increase in the number of physicians performing spirometry rather than a higher volume of services performed per physician. The substitution of flow-volume loops, for which the fee is higher, for simple spirograms also contributed to expenditure growth. There were wide regional variations in spirometry utilization. A small number of general practitioners and family physicians accounted for much of the regional variation. CONCLUSIONS The rapid growth in spirometry utilization may stem from the diffusion of inexpensive spirometers in physicians' offices and from increased awareness of guidelines promoting the use of flow measurements. However, the wide regional variation in utilization may indicate either incomplete implementation of spirometry guidelines or lack of direction on the appropriate frequency of spirometry use. Clearer, evidence-based guidelines and an implementation strategy are needed. Also required is further study of possible inadequate access to spirometry in low-use regions and inappropriate use in high-use regions, where spirometry use is concentrated among a small number of physicians.
The influence of volatile semiochemicals from stink bug eggs and oviposition-damaged plants on the foraging behaviour of the egg parasitoid Telenomus podisi

Abstract During host selection, physical and chemical stimuli provide important cues that modify search behaviours of natural enemies. We evaluated the influence of volatiles released by eggs and egg extracts of the stink bug Euschistus heros and by soybean plants treated with the eggs and egg extracts on Telenomus podisi foraging behaviour. Responses to volatiles were evaluated in Y-tube olfactometers after exposure to (1) one egg cluster for 24 h; (2) plants with eggs laid by the stink bug, tested at 24, 48, and 72 h after treatment; (3) plants with eggs laid artificially, tested at 24, 48, and 72 h after treatment; and (4) plants treated with acetone or hexane extracts of eggs. Telenomus podisi was attracted to volatiles emitted by one egg cluster and to acetone extracts of one egg cluster, but not to air or acetone controls. There were no responses to odours of plants treated with eggs or egg extracts. Analysis of acetone extracts of egg clusters by gas chromatography revealed the major components were saturated and unsaturated fatty acids, including hexadecanoic acid, linoleic acid, and (Z)-9-octadecenoic acid. Our results suggest that one egg cluster and the acetone extract of one egg cluster contain volatile compounds that can modify T. podisi foraging behaviour, and that the amounts of these compounds, probably together with some minor compounds, are important for host recognition by T. podisi. Also, the oviposition damage or egg extracts on the plant did not elicit indirect defences that attracted Telenomus podisi.
The Good Life of the Powerful

A common cliché and system-justifying stereotype is that power leads to misery and self-alienation. Drawing on the power and authenticity literatures, however, we predicted the opposite relationship. Because power increases the correspondence between internal states and behavior, we hypothesized that power enhances subjective well-being (SWB) by leading people to feel more authentic. Across four surveys representing markedly different primary social roles (general, work, romantic-relationship, and friendship surveys; Study 1), and in an experiment (Study 2a), we found consistent evidence that experiencing power leads to greater SWB. Moreover, authenticity mediated this effect. Further establishing the causal importance of authenticity, a final experiment (Study 2b), in which authenticity was manipulated, demonstrated that greater authenticity directly increased SWB. Although striving for power lowers well-being, these results demonstrate the pervasive positive psychological effects of having power, and indicate the importance of spreading power to enhance collective well-being.
Activity-Dependent Depression of Excitability and Calcium Transients in the Neurohypophysis Suggests a Model of “Stuttering Conduction”

Using millisecond time-resolved optical recordings of transmembrane voltage and intraterminal calcium, we have determined how activity-dependent changes in the population action potential are related to a concurrent modulation of calcium transients in the neurohypophysis. We find that repetitive stimulation dramatically alters the amplitude of the population action potential and significantly increases its temporal dispersion. The population action potentials and the calcium transients exhibit well correlated frequency-dependent amplitude depression, with broadening of the action potential playing only a limited role. High-speed camera recordings indicate that the magnitude of the spike modulation is uniform throughout the neurohypophysis, thereby excluding propagation failure as the underlying mechanism. In contrast, temporal dispersion and latency of the population spike do increase with distance from the stimulation site. This increase is enhanced during repeated stimulation and by raising the stimulation frequency. Changes in Ca influx directly affect the decline in population spike amplitude, consistent with electrophysiological measurements of the local loss of excitability in nerve terminals and varicosities, mediated by a Ca-activated K conductance. Our observations suggest a model of “stuttering conduction”: repeated action potential stimulation causes excitability failures limited to nerve terminals and varicosities, which account for the rapid decline in the population spike amplitude. These failures, however, do not block action potential propagation but generate the cumulative increases in spike latency.
A Three-dimensional Evaluation of the Effects of Functional Occlusal Forces on the Morphology of Dental and Periodontal Tissues of the Rat Incisor

We examined the effect of function on tooth and periodontal ligament (PDL) morphology in 40 lower incisors of adult female rats. Ten teeth were exposed to occlusal hyperfunction for three months, ten to hypofunction for three weeks, ten to hypofunction for three months, while ten teeth in normal occlusion served as control. Transverse ground sections were cut at various levels perpendicular to the tooth long axis, and their distances from the apex were calculated. The outlines of the tissues were traced and fed into a computer. We plotted the measurements according to their location and fitted them by second-order polynomials. We calculated tissue volume for the proximal 18 mm of bone-embedded tooth. Hyperfunction affected tooth shape, in that it became more rounded. The volumes of the dental tissues remained unchanged, while width and volume of the cementum-bordering PDL increased. Hypofunction did not alter tooth shape, but influenced its size: After three weeks, tooth circumference decreased, and after three months, it expanded. Dentin width was reduced, with concomitant increase of pulp size. The amount of enamel diminished initially, but after three months returned to normal values. The PDL bordering enamel expanded proportionally to the duration of hypofunction. The changes in socket size reflected the total dimensional variations in the tooth and its PDL. The results demonstrate that the shape and size of growing teeth and their periodontium are influenced by functional occlusal forces.
Preliminary Experience with a New Chemotherapy Regimen for Adults with Acute Lymphoblastic Leukemia

We treated 11 consecutive adult patients who presented with acute lymphoblastic leukemia to the University of Chicago with a novel, intensified chemotherapy regimen to evaluate the feasibility and toxicity of this program. Following a 5-drug induction therapy (course A), patients received sequential courses (B and C) of high-dose antimetabolite therapies, in part to replace cranial irradiation for CNS prophylaxis. All patients achieved a complete remission. The regimen was designed with a goal of administering all post-remission therapy in the outpatient setting. With the exception of the initial induction course (A), 31/38 total patient courses were administered in the outpatient clinic. As expected, the induction and consolidation courses (A and B) of this regimen were associated with grade 4 hematologic toxicity and significant but manageable infectious toxicity. Another novel aspect of the regimen included a combined intravenous and oral dosing schedule designed to sustain methotrexate levels × 1.0 μM-for 30 hours (course C). There was minimal toxicity with the CNS prophylaxis/high-dose methotrexate course (C). Methotrexate levels at 30 hours ranged from 0.31–4.0 μM, with a median of 1.0 μM (n=37). This study demonstrates that this novel post-remission regimen is feasible in adults and that high concentrations of methotrexate can be sustained in the outpatient setting. The Cancer and Leukemia Group B is presently evaluating the efficacy of this regimen in a large phase II trial (CALGB study 19802).
Mechanism for optimally trading off revenue and efficiency in multi-unit auctions

We study auctioning multiple units of the same good to potential buyers with single unit demand (i.e. every bidder wants only one unit of the good). Depending on the objective of the seller, different selling mechanisms are desirable. The Vickrey auction with a truthful reserve price is optimal when the objective is efficiency - allocating the units to the parties who values them the most. The Myerson auction is optimal when the objective is the seller's expected utility. These two objectives are generally in conflict, and cannot be maximized with one mechanism. In many real-world settings---such as privatization and competing electronic marketplaces---it is not clear that the objective should be either efficiency or seller's expected utility. Typically, one of these objectives should weigh more than the other, but both are important. We account for both objectives by designing a new deterministic dominant strategy auction mechanism that maximizes expected social welfare subject to aminimum constraint on the seller's expected utility. This way the seller can maximize social welfare subject to doing well enough for himself.
Mobility and Quality of Life after Discharge from a Clinical Geriatric Setting Focused on Gender and Age

Purpose: The purpose of this study was to assess the long‐term functional status and quality of life of older individuals with functional mobility impairment. Design: A prospective longitudinal study was conducted. Methods: The study sample consisted of elderly patients who had been admitted to a rehabilitation facility in Hamburg, Germany. Data were collected from February 2008 to August 2009 in the rehabilitation center and the living environment of the participants after discharge. Quality of life was assessed using the WHO Quality of Life‐BREF; activities of daily living were measured using the Barthel‐Index (BI). Findings: Functional status and overall quality of life increased from admission (BI = 73.33) to 6 months postadmission (BI = 89.29) but decreased at 12 months postadmission (BI = 85.71). Quality of life significantly increased in the psychological domain from admission (57.85) to 6 months follow‐up (67.85) (p = .010). Men showed a better functional status over time (p = .010) than women. Higher quality of life scores were associated with higher MMSE (p = .031) and self‐efficacy scores (p = .019) at admission. Conclusion: The findings of this study suggest a need for interventions to improve functional status and quality of life in this population, especially following 6 months after discharge. Clinical Relevance: Special interventions should address physical quality of life and physical functioning. Gender differences should be considered in planning and implementing programs.
MYCN oncogene amplification in neuroblastoma is associated with worse prognosis, except in stage 4s: the Italian experience with 295 children.

PURPOSE To evaluate the prognostic role of MYCN oncogene amplification in children with neuroblastoma. PATIENTS AND METHODS Of 694 children (age, 0 to 15 years) with previously untreated neuroblastoma, 295 (42%) were evaluated at diagnosis for MYCN gene amplification. RESULTS Clinical characteristics and survival results of 295 patients studied and 399 not studied for MYCN were comparable. In 48 of 295 patients studied for MYCN (16%), the gene was amplified (> or = three gene copies). Amplification was more frequent in children older than 1 year, with abdominal tumor (18% v 7%), advanced disease, normal vanillylmandelic (VMA) urinary excretion, and high lactate dehydrogenase (LDH), ferritin, and neuron-specific enolase (NSE) serum levels. In patients studied for MYCN, the 5-year overall survival (OS) rate was higher for children aged less than 1 year (90% v 44%), with extraabdominal tumor, stage 1 or 2 versus 3 versus 4, and normal NSE, LDH, and ferritin serum levels. Patients with amplified MYCN had a worse OS (odds ratio [OR], 3.38; confidence interval [CI], 2.22 to 5.16). This association held after adjustment for other characteristics. The impact of MYCN amplification was greater in patients with favorable characteristics, in particular age (OR, 10.28 for infants; 2.08 for older children) and stage (OR, 35.3 for stage 1 to 2; 8.41 for stage 3; 1.76 for stage 4). However, of 29 children with stage 4s, all three with amplified MYCN survive. In a multivariate analysis, the prognostic role of MYCN amplification, age, and stage was confirmed, but the size of the effect of MYCN was dependent on age and stage. CONCLUSION MYCN amplification is associated with a worse prognosis in children with neuroblastoma at all ages and stages except 4s. This association is most pronounced in children with otherwise favorable prognostic indicators, and in these children should be considered as an indication for more intensive intervention.
Self-management for Adult Patients With Cancer: An Integrative Review

Background: Individuals with cancer are surviving long term, categorizing cancer as a chronic condition, and with it, numerous healthcare challenges. Symptoms, in particular, can be burdensome and occur from prediagnosis through many years after treatment. Symptom severity is inversely associated with functional status and quality of life. Objective: Management of these millions of survivors of cancer in a stressed healthcare system necessitates effective self-care strategies. The purpose of this integrative review is to evaluate intervention studies led by nurse principal investigators for self-care management in patients with cancer. Methods: PubMed, CINAHL (Cumulative Index to Nursing and Allied health Literature), and the Cochrane Database were searched from January 2000 through August 2012. Search terms included “symptom management and cancer,” “self-management and cancer,” and “self-care and cancer. ” All articles for consideration included intervention studies with a nurse as the primary principal investigator. Results: Forty-six articles were included yielding 3 intervention areas of educational and/or counseling sessions, exercise, and complementary and alternative therapies. Outcomes were predominately symptom focused and often included functional status and quality of life. Few studies had objective measures. Overarching themes were mitigation, but not prevention or elimination of symptoms, and improved quality of life related to functional status. No one intervention was superior to another for any given outcome. Conclusions: Current interventions that direct patients in self-care management of symptoms and associated challenges with cancer/survivorship are helpful, but incomplete. No one intervention can be recommended over another. Implications for Practice: Guiding patients with cancer in self-care management is important for overall functional status and quality of life. Further investigation and tailored interventions are warranted.
Everyday Experiences of Place in the Kazakhstani Borderland: Russian Speakers Between Kazakhstan, Russia, and the Globe

Abstract This article analyzes different ways Russian speakers residing in Petropavlovsk, a city in northern Kazakhstan close to the border with Russia, make sense of the place they inhabit and form a sense of belonging to it. Politically and geographically speaking, Petropavlovsk serves as a contested area divided between Russian and Kazakhstani nation-building projects. While keeping in mind the antagonistic politics of both states, this research rather focuses on the everyday practices and personal narratives of Russian speakers living in this area. With the help of empirical material collected during the interviews and dwelling alongside the participants, this article demonstrates how through symbolic practices and everyday life experiences Russian speakers differently construct their understanding of Petropavlovsk as a Russian, Kazakh, and global space simultaneously.
Tracking retinal microgliosis in models of retinal ganglion cell damage.

PURPOSE To investigate the longitudinal profiles of microgliosis after optic nerve injury induced by optic nerve crush and acute elevation of intraocular pressure (IOP). METHODS A confocal scanning laser ophthalmoscope was used to image the retinal microglia of the CX3CR1(GFP/+) transgenic mice in vivo at baseline, 3 days and then weekly for 4 weeks after optic nerve crush (n = 3), and after elevating the IOP to 110 mm Hg for 30 (n = 3) or 60 (n = 3) minutes. RESULTS After optic nerve crush, the density of microglia increased by 2.43 ± 0.19-fold at week 1 and then gradually declined with 2.04 ± 0.24-, 1.69 ± 0.25-, and 1.29 ± 0.11-fold increases at week 2, 3, and 4, respectively. Microgliosis followed a similar pattern after acute IOP elevation and the increase in microglia was associated with the duration of IOP elevation. There were 1.35 ± 0.17- and 2.03 ± 0.08-fold increases in microglia at week 1, and 1.15 ± 0.11- and 1.11 ± 0.10-fold increases at week 4, after 30 and 60 minutes of acute IOP elevation, respectively. The morphology of microglia changed from ramified to ameboid form in 1 week, and then returned to ramified form in the subsequent weeks. There was a significant negative association between the number of surviving retinal ganglion cells (RGCs) and the extent of microgliosis during the follow-up period (R² = 0.72, P = 0.004). CONCLUSIONS Longitudinal in vivo imaging of the retinal microglia can provide an effective approach to study microgliosis and its association with RGC degeneration.
Distributive Justice and Customer Post-Complaint Behaviour of Fast Food Industry in Rivers State, Nigeria

The study examined the effect of distributive justice on customer post-complaint behaviour. The need for this study has become very demanding because a dissatisfied customer will imply that the company runs risk of operating at reduced state of future revenue stream accruable to the focal company. Data were drawn through questionnaire from 200 employees of 66 fast food firms in Rivers State. Descriptive and inferential statistics were utilized in analysing the data. The study found that distributive justice has a positive and significant relationship with customer post-complaint behaviour. In view of the above findings, the study concludes that customers who experience dissatisfaction will be willing to enact positive post-complaint behaviours if the firm addresses their issues satisfactorily; and recommend amongst others that management of fast food firms should be key in effective distributive justice in order to drive customers towards positive post-complaint behaviour.
The Impact of Business Group Affiliation on Stock Price Informativeness: Evidence from an Emerging Market

This paper examines the relationship between business group affiliation and stock price informativeness in an emerging market setting. We use stock price synchronicity as a measure, and study the impact of group affiliation &#8208;specifically the extent of affiliation, ownership structure and existence of group bank&#8208; on firm specific information content. Results reveal that the amount of firm&#8208;specific information capitalized into stock prices tends to be lower (higher) when the firm is group&#8208;affiliated (unaffiliated), indirectly (directly) owned, and affiliated group has (does not have) a group bank. Additionally, the extent of group affiliation maintains a non&#8208;linear relationship with synchronicity, suggesting that the perception of higher versus lower levels of group ownership differs.