Miracle-dz/UniFAR-FTUs huggingface dataset:
Chaperone Stress 70 Protein (STCH) Binds and Regulates Two Acid/Base Transporters NBCe1-B and NHE1*

Background: Regulation of intracellular pH is critical for cellular homeostasis. Results: Stress 70 protein chaperone (STCH) interacts with pH regulating transporters NBCe1-B and NHE1 and modulates their functional expression. Conclusion: STCH chaperone dynamically regulates intracellular pH through site-specific interactions with ion transporters. Significance: These novel STCH interactions provide a mechanism for intracellular pH regulation in response to homeostatic stress. Regulation of intracellular pH is critical for the maintenance of cell homeostasis in response to stress. We used yeast two-hybrid screening to identify novel interacting partners of the pH-regulating transporter NBCe1-B. We identified Hsp70-like stress 70 protein chaperone (STCH) as interacting with NBCe1-B at the N-terminal (amino acids 96–440) region. Co-injection of STCH and NBCe1-B cRNA into Xenopus oocytes significantly increased surface expression of NBCe1-B and enhanced bicarbonate conductance compared with NBCe1-B cRNA alone. STCH siRNA decreased the rate of Na+-dependent pHi recovery from NH4+ pulse-induced acidification in an HSG (human submandibular gland ductal) cell line. We observed that in addition to NBCe1-B, Na+/H+ exchanger (NHE)-dependent pHi recovery was also impaired by STCH siRNA and further confirmed the interaction of STCH with NHE1 but not plasma membrane Ca2+ ATPase. Both NBCe1-B and NHE1 interactions were dependent on a specific 45-amino acid region of STCH. In conclusion, we identify a novel role of STCH in the regulation of pHi through site-specific interactions with NBCe1-B and NHE1 and subsequent modulation of membrane transporter expression. We propose STCH may play a role in pHi regulation at times of cellular stress by enhancing the recovery from intracellular acidification.
Optimal Control of Medium-Voltage Drives—An Overview

Carrier modulation is the most common method for inverter control of ac drive systems. Operation at switching frequencies of several kilohertz is customary to restrain the harmonic distortion of the motor currents. Lower switching frequency is preferred for medium-voltage drives, owing to the higher switching losses of the semiconductor devices. This calls for optimizing the performance of the pulsewidth modulator. Improvements are achieved by abolishing carrier modulation with its equidistant time spacing of the voltage pulses. Optimal pulse patterns can be precalculated for every steady-state operating point of the drive. The patterns are stored in a memory of the drive system, from which they are retrieved and used for inverter control. Control at transient operation is achieved by adapting the optimal steady-state pulse patterns to the respective situation. Specific trajectories of the motor current space vector are then created by online optimization. An alternative method of online optimization relies on predicting the space vector trajectories for the next possible inverter switching states. The switching state that leads to minimum switching frequency is then selected. Optimal pulsewidth modulation reduces harmonic distortion. It permits operation at very low switching frequency and reduces the switching losses. This increases the current-carrying capability of the semiconductor devices to the extent that the power rating of an inverter approximately doubles.
Technology and evolution: a root and branch view of Asian iron from first-millennium bc Sri Lanka to Japanese steel

Abstract Evidence for a previously unrecognized pan-Asian metallurgical tradition of linear configuration iron-smelting furnaces is reviewed. The foundation of this technological lineage lies in an evolutionary series of excavated furnaces in Sri Lanka dating from the fourth century bc to the eleventh century ad. Further archaeological, ethnographic and documentary evidence from Burma, Cambodia, Sarawak and Japan demonstrates the spread of linear furnace technology and its association with the production of high-carbon steels, often associated with weapons manufacture. An evolutionary approach is used to argue that a process of memetic inheritance explains a major divergence in Eastern and Western metallurgical development and furnace design.
On Individual and Institutional Noise Trading

Previous research suggests that individual investor sentiment has incremental explanatory power for returns of small cap stocks, value stocks, stocks with low institutional ownership, and stocks with lower prices (Kumar and Lee (2003)) and that there is a strong link between institutional sentiment and the returns of large stocks (Brown and Cliff (2004)). With respect to return volatility, Jackson (2003a,b) found that larger trading share of individuals in certain stocks does not increase their subsequent volatility; however, the opposite is true for institutional participation, which increases conditional volatility. We propose an integrated framework that jointly tests for the effects of individual as well as institutional sentiment on return and volatility. Using implicit measures of sentiment for the German stock market over the period 02/2000 until 04/2005, our results suggest that institutional sentiment has only minor incremental explanatory power for returns and conditional volatility of large cap stocks, but we find strong evidence that individual sentiment is the important market-wide risk factor and does affect the return and conditional volatility of large as well as small cap stocks. JEL Codes: C22, D82.
Bizygomatic diameter: the thickness of the soft tissues over the zygions.

When comparisons are made between the bizygomatic diameter of different subjects, or when measurements made on the living are compared with those on skulls, the thickness of the soft tissue overlying the zygions is of considerable importance. The thickness of these tissues over each of 208 zygions of cadavera has been studied and has been found to differ considerably. The range was from 1.4 mm to 21.4 mm, and the thickness was related to the general body-build — fat, medium or thin. Statements in the literature assume that 6 mm is a suitable estimate of the total thickness of the soft tissues over both zygions. However the present findings indicate that these assumptions are not justified, for in 92% of these specimens the thickness exceeded 6 mm.
Texas Population Substructure and Its Impact on Estimating the Rarity of Y STR Haplotypes from DNA Evidence *

Abstract: Three sampled populations of unrelated males—African American, Caucasian, and Hispanic, all from Texas—were typed for 16 Y short tandem repeat (STR) markers using the AmpFlSTR® YfilerTM kit. These samples also were typed previously for the 13 core CODIS autosomal STR loci. Most of the 16 marker haplotypes (2478 out of 2551 distinct haplotypes) were observed only once in the data sets. Haplotype diversities were 99.88%, 99.89%, and 99.87% for the African American, Caucasian, and Hispanic sample populations, respectively. FST values were very small when a haplotype comprised 10–16 markers. This suggests that inclusion of substructure correction is not required. However, haplotypes consisting of fewer loci may require the inclusion of FST corrections. The testing of independence of autosomal and Y STRs supports the proposition that the frequencies of autosomal and Y STR profiles can be combined using the product rule.
Competitive enzyme immunoassays for the rapid detection of antibodies to feline infectious peritonitis virus polypeptides

Monoclonal antibodies specific for the envelope (E1), peplomer (E2), and nucleocapsid (N) polypeptides of feline infectious peritonitis virus (FIPV) were used in rapid, competitive enzyme-linked immunosorbent assays (ELISA) to study the humoral immune response of cats to FIPV infection. Results from the competitive ELISAs were correlated with those from immunofluorescent antibody assays (IFAs) on 203 samples obtained from 64 individual cats. The IFA results correlated best with those obtained with the anti-E1 specific competitive ELISA (85.7%). In contrast, anti-N and anti-E2 competitive ELISA results correlated with IFA results only 65.5 and 2.4% of the time, respectively. The results of the anti-E1 specific competitive ELISA were not influenced by the total immunoglobulin concentration or the possible presence of free viral antigens in the serum. These results suggest that a competitive ELISA involving the use of enzyme-conjugated monoclonal antibody to the E1 glycoprotein of FIPV is a simple and rapid replacement for the more cumbersome IFA.
A comparison of performance of the Ovsynch treatment program between cycling and non-cycling cows within seasonally-calving dairy herds.

OBJECTIVE To compare performance of the Ovsynch program on reproductive performance between cycling and non cycling cows in seasonally-calving herds. PROCEDURE An Ovsynch mating program (100 mg Gonadorelin on day 1 and day 9, 500 mg of Cloprostenol on day 7 with fixed time artificial insemination on day 10) was administered to 3,559 cows from 14 herds in Australia and New Zealand. Cycling status before planned start of mating was determined. All cows were treated and artificial insemination continued for at least 25 days after fixed time artificial insemination. Pregnancy testing was performed 75 to 100 days after fixed time artificial insemination. Multivariable modelling examined the impact of the Ovsynch program and other risk factors upon reproductive performance. RESULTS Thirty percent of cows were classified as no visible oestrous (NVO). Odds of being NVO increased significantly for cows that were young, recently calved, and in low body condition. The fixed time artificial insemination conception rate was 35.7% and 33.2%, 21-day pregnancy rate was 54.5% and 48.4% and 42-day pregnancy rate was 69.7% and 62.6% for cycling and NVO cows respectively. Odds of pregnancy increased significantly for cows calved more than 40 days by planned start of mating, in greater body condition, and cycling, and there was a significant interaction between body condition and cycling status in both models. The return-to-service rates by 24-days were 67.6% and 55.9% and by the end of the AI period were 86.9% and 81.5% for cycling and NVO cows respectively. Odds of return to service increased significantly for cows in greater condition score in both models. Odds of return were increased for cycling cows in the 24-day multivariable model. CONCLUSION The Ovsynch program may provide a useful treatment option for NVO cows within seasonally-calving pasture-based dairy herds.
Diffuse FDG shoulder uptake on PET is associated with clinical findings of osteoarthritis.

OBJECTIVE Our objective was to examine the degree and pattern of (18)F-FDG uptake within the shoulder as a potential marker of joint inflammation or injury. SUBJECTS AND METHODS Twenty-four patients undergoing (18)F-FDG PET for clinical oncologic assessment completed questionnaires regarding history of shoulder disease, trauma, pain, and/or functional impairment. Thorough physical examination of the shoulder was performed. A clinical diagnosis of specific shoulder derangement or normal was established for each patient. PET scans were evaluated blindly by a nuclear medicine physician and a musculoskeletal radiologist qualitatively for location, distribution, and intensity of shoulder uptake. Standardized uptake values (SUV) were measured. RESULTS Twenty-one patients had shoulder PET findings. Fourteen had clinical findings consistent with a specific diagnosis in the PET-positive shoulder. The remaining seven PET-positive patients were clinically normal. Three recognizable patterns of uptake were appreciable. Eight of 10 patients with diffuse uptake had findings of osteoarthritis (n = 7) or bursitis (n = 1). Two of four patients with focal greater tuberosity uptake had findings of rotator cuff injury. Two of four patients with focal glenoid uptake had findings of frozen shoulder. SUV showed a positive correlation with subject age (p < 0.01), but no association with clinical findings was identified. CONCLUSION The pattern of FDG uptake within the shoulder may point to specific clinical entities. While focal uptake is less reliably related to clinical findings, diffuse uptake is associated with signs and symptoms of osteoarthritis or bursitis.
Approximate Nearest Neighbors Search Without False Negatives For l_2 For c>sqrt{loglog{n}}

In this paper, we report progress on answering the open problem presented by Pagh~[14], who considered the nearest neighbor search without false negatives for the Hamming distance. We show new data structures for solving the $c$-approximate nearest neighbors problem without false negatives for Euclidean high dimensional space $\mathcal{R}^d$. These data structures work for any $c = \omega(\sqrt{\log{\log{n}}})$, where $n$ is the number of points in the input set, with poly-logarithmic query time and polynomial preprocessing time. This improves over the known algorithms, which require $c$ to be $\Omega(\sqrt{d})$. This improvement is obtained by applying a sequence of reductions, which are interesting on their own. First, we reduce the problem to $d$ instances of dimension logarithmic in $n$. Next, these instances are reduced to a number of $c$-approximate nearest neighbor search instances in $\big(\mathbb{R}^k\big)^L$ space equipped with metric $m(x,y) = \max_{1 \le i \le L}(\lVert x_i - y_i\rVert_2)$.
A practical method for measuring macular pigment optical density.

PURPOSE Increasing evidence indicates that the macular pigments (MP) protect the central retina and may retard macular disease. For that reason, a practical method for measuring MP that does not require elaborate optics and can be applied to diverse populations by operators with a modest amount of experience was developed and validated. METHODS A small tabletop device based on light-emitting diodes (LEDs) as the light source with electronic controls was constructed. Macular pigment was measured with the tabletop device with a 1 degrees test stimulus at 460 nm using heterochromatic flicker photometry, and the results were compared with measurements using a traditional three-channel Maxwellian view system with a xenon-arc source. RESULTS Macular pigment density of 30 subjects (age range, 16-60 years) was measured with both stimulus systems. Macular pigment measured with the LED tabletop device in free view was highly correlated with MP measured in Maxwellian view (y = -0.03 + 1.06x, r = +0.95). The average absolute difference between the two techniques was 0.04 (SD, 0.03). The new technique was not significantly affected by variations in lens optical density, pupil size, or small head movements. CONCLUSIONS Psychophysical measurement of MP provides a unique opportunity to make repeated noninvasive assessment of the concentration of a protective nutrient in the retina. The availability of this new device should make this measurement technology accessible to a wide variety of investigators for application to diverse populations.
Magnetic resonance neurography and diffusion tensor imaging of the peripheral nerves in patients with Charcot‐Marie‐Tooth Type 1A

Investigation of peripheral neuropathies by magnetic resonance neurography (MRN) may provide increased diagnostic accuracy when performed in combination with diffusion tensor imaging (DTI). This study seeks to evaluate DTI in the detection of neuropathic abnormalities in Charcot‐Marie‐Tooth type 1A (CMT1A).
A Novel Case of Concurrent Renal Tumors

Renal cell carcinoma of chromophobe type is a malignant neoplasm with characteristic large cells that exhibit prominent cell membranes. Primary renal carcinoid tumors are rare tumors with the histological appearance similar to well-differentiated neuroendocrine tumors at other sites. Chromophobe renal cell carcinoma with neuroendocrine differentiation is exceedingly rare. The authors describe a case of a chromophobe renal cell carcinoma and carcinoid tumor of the kidney presenting as distinct masses in the same kidney in a 34-year-old male. The histologic and immunohistochemical features of both the tumors were characteristic with no overlapping features. The carcinoid tumor presented at a higher stage with nodal metastasis. To the best of the authors’ knowledge, this is the first case of 2 morphologically distinct tumors, one chromophobe renal cell carcinoma and the other primary carcinoid tumor in the same kidney.
Endangered languages: Technical, emotional, and ideological issues in reversing language shift: examples from Southeast Alaska

Introduction Native American people across the nation have expressed concern over the loss of indigenous languages, and they have expressed a desire to do something to prevent it. The loss of small, tribal languages is a worldwide phenomenon and an international concern. In Alaska, only two of the twenty Native languages are now being learned by children in the traditional way of parents speaking to children (Krauss 1995b 12–3). If this trend continues, the other eighteen languages will eventually die. Unfortunately, it is not easy to understand and appreciate the process by which languages are lost, the actions we must take to prevent such loss, or the actions we must take to preserve and/or restore endangered languages. This has been a difficult chapter to write because most of the examples are of failures: things that have gone wrong or that have not worked. We have been personally involved in the dynamics described in this chapter in language research, teacher training, and materials development. As part of that picture, we have contributed to the failures as well as the successes in the general language-restoration effort of the last quarter century. But this chapter is composed in the spirit of optimism. Its goal is to involve all of us in values clarification and to unite us in making informed choices and in taking timely and appropriate action. Hopefully, we all can learn through our mistakes and realign our courses while there is still time.
The Birth of Hedonism: The Cyrenaic Philosophers and Pleasure as a Way of Life

Kurt Lampe's The Birth of Hedonism: The Cyrenaic Philosophers and Pleasure as a Way of Life is a welcome addition to the literature on ancient hedonism and to our understanding of a lesser-known movement in Greek ethics. It is the first monograph on Cyrenaic ethics in English and, as Lampe claims, the first "systematic reconstruction of Cyrenaic ethics within its own historical contexts" in any language. (2-3)
Traditional versus up-front [18F] fluorodeoxyglucose-positron emission tomography staging of non-small-cell lung cancer: a Dutch cooperative randomized study.

PURPOSE We investigated whether application of positron emission tomography (PET) immediately after first presentation might simplify staging while maintaining accuracy, as compared with traditional strategy in routine clinical setting. METHODS At first presentation, patients with a provisional diagnosis of lung cancer without overt dissemination were randomly assigned to traditional work-up (TWU) according to international guidelines or early PET followed by histologic/cytologic verification of lesions, or imaging and follow-up. Patients with [18F] fluorodeoxyglucose (18FDG) -avid, noncentral tumors without suspicion of mediastinal or distant metastases on PET proceeded directly to thoracotomy. Follow-up in presumed benign lesions was at least 12 months. In patients treated with surgery or neoadjuvant therapy, the quality of staging was measured by comparing the clinical stage to the final stage (combination of peroperative staging and 6 months of follow-up). To investigate test substitution, we analyzed the number of (non)invasive tests to achieve clinical TNM staging, and its associated costs. RESULTS Between 1999 and 2001, 465 patients (233 TWU, 232 PET) were enrolled at 22 hospitals. The mean (standard deviation) number of procedures to finalize staging was equal in the TWU arm and the PET arm: 7.9 (2.0) v 7.9 (1.9), P = . 90, respectively. Mediastinoscopies occurred significantly less often in the PET arm. Agreement between clinical and final stage was good in both arms (kappa = .85 v .78; P = .07). Costs did not differ significantly. CONCLUSION Up-front 18FDG-PET in patients with (suspected) lung cancer does not reduce the overall number of diagnostic test, but it maintains quality of TNM staging with the use of less invasive surgery.
Impact of appropriate empirical antimicrobial therapy on outcome of dogs with septic peritonitis.

OBJECTIVE To determine whether appropriate empirical antimicrobial therapy influenced survival in dogs with septic peritonitis. DESIGN Retrospective case series (2003-2011). SETTING University teaching hospital. ANIMALS Eighty-six dogs with cytological confirmation or positive bacterial culture of abdominal sepsis and subsequent surgical intervention. INTERVENTIONS None. MEASUREMENT AND MAIN RESULTS Forty-nine of 86 dogs (57%) survived to hospital discharge. Thirty-seven of 86 dogs were classified as having ''abdominal infection,'' 31/86 as ''severe sepsis,'' and the remaining 18/86 as in ''septic shock. '' Mortality was greatest in the ''septic shock'' category (94%). Empirical antimicrobial treatments were appropriate in 41/78 dogs (52.6%). Appropriateness was not associated with treatment outcome overall or when compared between sepsis severity groups. Antimicrobials had been given in the 30 days before admission in 63/86 (73.3%) dogs. Prior therapy with antimicrobials showed no association with outcome (P = 0.512) but was associated with subsequent inappropriate empirical antimicrobial selection (P = 0.031). Recent abdominal surgery was associated with subsequent inappropriate empirical antimicrobial selection (P = 0.021). CONCLUSIONS In this population, appropriateness of empirical antimicrobial choice was not associated with survival to discharge. Previous antimicrobial administration or abdominal surgery was associated with subsequent inappropriate empirical antimicrobial selection.