Miracle-dz/UniFAR-FTUs huggingface dataset:
High Stature in Neonatal Myotubular Myopathy

A male baby was delivered at 40 weeks of gestation. His birthweight was 3300 g (50th percentile) and length 57 cm (over the 97th percentile). Parents measured 1.70 m and 1.83 m, respectively. The mother later gave birth to two healthy infants, a boy and a girl who measured 53 cm and 47 cm and weighed 3800 g and 2700 g at 40 weeks and 39 weeks of gestation, respectively. In our patients the chest X-ray at birth showed the characteristic thin ribs (Fig. 1). A diagnosis of neuromuscular disease was rapidly made on the basis of persistent generalized hypotonia, muscle atrophy, and feeble respiratory efforts. Muscle biopsies taken from the biceps on the 8th day revealed the characteristic features of myotubular myopathy with predominance of type I fibres and persistence of fetal myotubes in a large proportion of the muscle fibers (Fig. 2). The baby died at 2 months of age from respiratory insufficiency. His mother was clinically normal and her muscle biopsy did not show any abnormality. Because of the high stature observed in this patient, previously published cases of neonatal myotubular myopathy were reviewed and birth length parameters were plotted on Lubchenco growth curves. Only in 6 previously reported cases (14) the height had been recorded. In four of the 6 infants the height was over the 90th percentile, and all had a decreased weight for height (Fig. 3).
Genetic control of estrogen‐regulated transcriptional and cellular responses in mouse uterus

The uterotropic response of the uterus to 17β‐estradiol (E2) is genetically controlled, with marked variation observed depending on the mouse strain studied. Previous genetic studies from our laboratory using inbred mice that are high [C57BL/6J (B6)] or low [C3H/HeJ (C3H)] responders to E2 led to the identification of quantitative trait (QT) loci associated with phenotypic variation in uterine growth and leukocyte infiltration. The mechanisms underlying differential responsiveness to E2, and the genes involved, are unknown. Therefore, we used a microarray approach to show association of distinct E2‐regulated transcriptional signatures with genetically controlled high and low responses to E2 and their segregation in (C57BL/6J×C3H/HeJ) F1 hybrids. Among the 6664 E2‐regulated transcripts, analysis of cellular functions of those that were strain specific indicated C3H‐selective enrichment of apoptosis, consistent with a 7‐fold increase in the apoptosis indicator CASP3, and a 2.4‐fold decrease in the apoptosis inhibitor Naip1 (Birc1a) in C3H vs. B6 following treatment with E2. In addition, several differentially expressed transcripts reside within our previously identified QT loci, including the ERα‐tethering factor Runx1, demonstrated to enhance E2‐mediated transcript regulation. The level of RUNX1 in uterine epithelial cells was shown to be 3.5‐fold greater in B6 compared to C3H. Our novel insights into the mechanisms underlying the genetic control of tissue sensitivity to estrogen have great potential to advance understanding of individualized effects in physiological and disease states. —Wall, E. H. , Hewitt, S. C. , Liu, L. , del Rio, R. , Case, L. K. , Lin, C. ‐Y. , Korach, K. S. , Teuscher, C. Genetic control of estrogen‐regulated transcriptional and cellular responses in mouse uterus. FASEB J. 27, 1874–1886 (2013). www.fasebj. org
Presence and short‐term persistence of SARS‐CoV‐2 neutralizing antibodies in COVID‐19 convalescent plasma donors

In March 2020, the Food and Drug Administration (FDA) approved use of COVID‐19 convalescent plasma (CCP) as an investigational new drug for treatment of COVID‐19. Since then, collection of CCP from COVID‐19–recovered patients has been implemented in donor centers nationwide. Childrenʼs Hospital Colorado rapidly put into practice a CCP collection protocol, necessitating development and implementation of assays to evaluate SARS‐CoV‐2 antibodies in CCP units.
Characterization of RarA, a Novel AraC Family Multidrug Resistance Regulator in Klebsiella pneumoniae

ABSTRACT Transcriptional regulators, such as SoxS, RamA, MarA, and Rob, which upregulate the AcrAB efflux pump, have been shown to be associated with multidrug resistance in clinically relevant Gram-negative bacteria. In addition to the multidrug resistance phenotype, these regulators have also been shown to play a role in the cellular metabolism and possibly the virulence potential of microbial cells. As such, the increased expression of these proteins is likely to cause pleiotropic phenotypes. Klebsiella pneumoniae is a major nosocomial pathogen which can express the SoxS, MarA, Rob, and RamA proteins, and the accompanying paper shows that the increased transcription of ramA is associated with tigecycline resistance (M. Veleba and T. Schneiders, Antimicrob. Agents Chemother. 56:4466–4467, 2012). Bioinformatic analyses of the available Klebsiella genome sequences show that an additional AraC-type regulator is encoded chromosomally. In this work, we characterize this novel AraC-type regulator, hereby called RarA (Regulator of antibiotic resistance A), which is encoded in K. pneumoniae, Enterobacter sp. 638, Serratia proteamaculans 568, and Enterobacter cloacae. We show that the overexpression of rarA results in a multidrug resistance phenotype which requires a functional AcrAB efflux pump but is independent of the other AraC regulators. Quantitative real-time PCR experiments show that rarA (MGH 78578 KPN_02968) and its neighboring efflux pump operon oqxAB (KPN_02969_02970) are consistently upregulated in clinical isolates collected from various geographical locations (Chile, Turkey, and Germany). Our results suggest that rarA overexpression upregulates the oqxAB efflux pump. Additionally, it appears that oqxR, encoding a GntR-type regulator adjacent to the oqxAB operon, is able to downregulate the expression of the oqxAB efflux pump, where OqxR complementation resulted in reductions to olaquindox MICs.
Synthesis and characterization of CdS nanoparticles stabilized by double hydrophilic block copolymers.

A novel and simple preparation method for single monodisperse CdS nanoparticles with controllable size and stability against oxidation is presented. Double-hydrophilic block copolymers consisting of a solvating poly(ethylene glycol) PEG block and a poly(ethylene imine) PEI binding block have been used as effective stabilizers for the solution synthesis of high-quality CdS nanoparticles in water and methanol. Absorption, fluorescence spectroscopy, and transmission electron microscopy were employed for characterization, which revealed that the prepared CdS nanoparticles had a well-resolved cubic structure and were monodisperse in size. It was also found that the CdS nanoparticles were dispersed in solution as single entities and showed a very good resistance against oxidation for months, according to their polymer shell. The particle size was controllable in the range between 2 and 4 nm by adjusting the polymer concentration and choice of the solvent.
Recruitment of Atlantic croaker, Micropogonias undulatus: Do postsettlement processes disrupt or reinforce initial patterns of settlement?*

Understanding the rela- tive importance of pre- and postsettle- ment processes is critical to under- standing the population dynamics of marine fishes. Our goals in this study were 1) to examine habitat preference and habitat use of newly settled Atlan- tic croaker, Micropogonias undulatus, and 2) to determine if postsettlement growth or predation varied with habi- tat type. Field surveys showed no dif- ference in croaker abundance among three estuarine habitats: marsh edge, seagrass, and sand. Behavioral experi- ments in laboratory mesocosms sug- gested that the pattern of similar use of habitats in the field results from a lack of preference among habitats. In a field experiment, croaker recruitment was greater to artificial seagrass than to sand habitats, but there was no dif- ference in fish density in habitats with or without food supplementation. More- over, growth rates were similar in both sand and artificial seagrass habitats and in habitats with or without food supplementation. In a second experi- ment, we were unable to detect a dif- ference in the density of newly settled croaker between sand and artificial
Nosocomial Spread of a Staphylococcus capitis Strain with Heteroresistance to Vancomycin in a Neonatal Intensive Care Unit

ABSTRACT A premature infant in a neonatal intensive care unit (NICU) developed a bloodstream infection caused by coagulase-negative staphylococci (CoNS) sensitive to vancomycin. The infection persisted for 3 weeks, despite therapy with vancomycin and replacement of all intravenous catheters. The neonate died due to necrotizing enterocolitis which developed during the ongoing sepsis. We screened this strain and 216 other strains of CoNS from cultures of blood obtained from neonates between 1997 and 2000 for heteroresistance to vancomycin. Forty-eight isolates, including the strain that caused ongoing sepsis, proved heteroresistant. All isolates were identified as Staphylococcus capitis and were identical, just as their resistant stable subcolonies were, when they were genetically fingerprinted by amplified-fragment length polymorphism analysis. The heteroresistant phenotype of this endemic strain was confirmed by population analysis. We conclude that heteroresistance to vancomycin occurs in S. capitis and might be the cause of therapeutic failures in NICUs. Moreover, heteroresistant strains can become endemic in such units.
Effect of Land Use Change on Carbon Content and CO 2 Flux of Cloud Forest Soils, Santa Elena, Costa Rica

We investigated the effects of land-use changes on soil carbon storage and soil CO2 flux by comparing soils from mature cloud forest and 31-year-old secondary forest, both in the Santa Elena Forest Reserve, a municipallyowned reserve at an elevation of 1600 to 1700 m near the town of Monteverde, and a clear-cut pasture near the reserve. Soils in the mature forest exhibit only weak horizonation but typically thick A horizons; they also consistently yield the highest carbon contents in the upper 30 cm. Soil CO2 flux was the highest in these soils, but also displayed the highest spatial variability. Secondary forest soils contain substantially less soil carbon than mature forest soils, but more than pasture soils. CO2 flux in the secondary forest soils was more similar to that of the mature forest, but displayed lower spatial variability. The pasture soils contain less soil carbon and produced lower CO2 flux levels than either of the forest soils. The pasture soils typically contain a well-defined coarse sandy layer 10 to 20 cm below the surface that we interpret as a sediment layer deposited across much of the landscape following a widespread erosion event, likely a consequence of the clear-cutting. Soil nitrogen concentrations are more than an order of magnitude lower than soil carbon concentrations, and display no trends between the different landscapes examined. Our preliminary results suggest that reforestation does restore soil carbon to clear-cut landscapes, but returning soil carbon levels to pre-land use levels occurs at a time scale of centuries, rather than decades.
Social value and content value in social media: Two paths to psychological well-being

ABSTRACT The unique characteristics and universal popularity of social media enable consumers to experience their customer value and thus improve their psychological well-being. Hence, it is important for researchers to investigate how consumers experience their customer value in this new media and demonstrate how managers can use the ensuing knowledge for designing appropriate marketing strategies. This article is a step toward that direction. We explore the antecedents and consequences of customer value in social media so as to provide a deeper insight into consumer behavior and subsequently discuss its managerial implications. Specifically, we develop a model that incorporates psychological well-being as the endogenous variable, interdependence self-construal and independent self-construal as exogenous variables, and social value, content value, social identity, self-esteem, and flow as mediating variables. Based on data from a sample of 437 social media consumers collected by an online survey and through analysis of the data by SPSS 22.0 and Amos 22.0 programs, the study revealed that consumers can gain psychological well-being by either of two paths: consumers with a higher degree of interdependent self-construal will have a higher degree of social value experience, thus leading to a higher degree of psychological well-being through a positive mediating role of social identity; whereas consumers with a higher degree of independent self-construal will have a higher degree of content value experience, thus leading to a higher degree of psychological well-being through a positive mediating role of self-esteem. Managers can segment the consumers based on their self-construal and design appropriate customer relationship strategies.
An empirical analysis of the adoption of m-learning in Malaysia

This paper empirically examines the factors affecting the adoption of Mobile learning (m-learning) in Malaysia. The six factors examined in this study are perceived ease of use, perceived usefulness, technical feasibility, cost effectiveness, quality of services and cultural aspects. Data were collected from 181 Malaysian users. The hypotheses were analysed using multiple regression analysis. The results show that perceived ease of use, perceived usefulness, quality of services and cultural aspects have significant and positive effects on the adoption of m-learning in Malaysia. Educational institutions that are planning to adopt m-learning will be formulate adoption strategies based on this study's findings.
PTSD Symptom Trajectories in Disaster Volunteers: The Role of Self-Efficacy, Social Acknowledgement, and Tasks Carried Out.

Millions of volunteers respond after disasters, with a 24% to 46% risk of developing posttraumatic stress disorder (PTSD). It is unclear which symptom trajectories develop and how they differ between core (volunteering before the disaster) and noncore volunteers (joining after the disaster) and which factors predict trajectories. Symptoms of PTSD were assessed at 6-, 12-, and 18-months postearthquake in 449 volunteers in Indonesia. Demographics, previous mental health service use, self-efficacy, social acknowledgment, and type of tasks were assessed at 6 months. In both core and noncore volunteers, 2 PTSD symptom trajectories emerged: a resilient trajectory (moderate levels of symptoms with a slow decrease over time; 90.9%) and a chronic trajectory (higher levels of symptoms with an increase over time; 9.1%). In both trajectories, core volunteers had fewer symptoms than noncore volunteers. Core volunteers in the chronic trajectory were characterized by having sought prior mental help, reported lower levels of self-efficacy and social acknowledgment, and were more likely to have provided psychosocial support to beneficiaries (Cramér's V = .17 to .27, partial η(2) = . 02 to . 06). Aid organizations should identify and follow up chronic PTSD trajectories in volunteers, including the noncore, who may be out of sight to the organization after the acute response phase.
Different Aspects of Visual Impairment as Risk Factors for Falls and Fractures in Older Men and Women

Visual impairment has been implicated as a risk factor for falling and fractures, but results of previous studies have been inconsistent. The relationship between several aspects of vision and falling/fractures were examined in a prospective cohort study in 1509 older men and women. The analyses showed that impaired vision is an independent risk factor for both recurrent falling and fractures.
Difference in cell proliferation between two structurally different lesions in colorectal adenomas: high-grade dysplasia and carcinoma in situ.

BACKGROUND Despite the fact that most Western pathologists diagnose carcinoma in situ (CIS) in many organs the same pathologists generally diagnose similar histological aberrations in colorectal adenomas, as high-grade dysplasia (HGD). MATERIALS AND METHODS Five large colorectal adenomas (measuring > or =20 mm) having areas of both HGD and CIS on staining with hematoxylin and eosin (H&E) were assessed with the proliferation antibody Ki-67 (clone MIB1). RESULTS HGD is built of tightly packed, spindle shaped, hyperchromatic cells with moderate pleomorphic nuclei having coarse chromatin and CIS of marked pleomorphic, vesicular, hypochromatic nuclei with a prominent nucleolus. Ki-67 was expressed in 96% of the HGD cells but only in 3.5% of the CIS cells (p<0.05). CONCLUSION The results of this and of previous investigations using the DNA-specific Feulgen stain, suggest that HGD and CIS in colorectal adenomas are two dissimilar morphological entities with dissimilar molecular behaviour. HGD cells in colorectal adenomas seem to be proliferating at any given time, whereas the majority of the CIS cells are not.
The Effect of Glycolic Acid on the Treatment of Acne in Asian Skin

BACKGROUND Glycolic add has become important and popular for treating acne. OBJECTIVE To evaluate the efficacy and safety of serial glycolic acid peels with glycolic acid home care products on facial acne lesions and other associated skin problems. METHODS We collected 40 Asian candidates with moderate to moderately severe acne. They were divided into two groups according to the degree o of greasiness of their facial skin. The two groups’ members were treated with four series of 35% and 50% glycolic acid peels, respectively. They also used 15% glycolic acid home care products during this study period. The improvement of acne as well as other associated problems were assessed by both the physicians and the patient themselves. RESULTS Significant resolution of comedones, papules, and pustules was found. The skin texture of each candidate was dramatically rejuvenated. Consistent and repetitive treatment with glycolic acid was needed for the apparent improvement of acne scars and cystic lesions. The follicular pores also became comparatively smaller. Furthermore, most of the candidates had much brighter and lighter looking skin. Only small percentage of patients (5.6%) developed side effects. CONCLUSION Glycolic acid has considerable therapeutic value for acne with minimal side effects even in Asian skin. It may be an ideal adjunctive treatment of acne.
Quantitative effect of tenorrhaphy on intrinsic vasculature of the equine superficial digital flexor tendon.

OBJECTIVE To compare effects of the locking-loop suture pattern (LLP) and 3-loop pulley (3LP) suture pattern for tenorrhaphy on the intrinsic vasculature of the superficial digital flexor tendon (SDFT) of horses in vitro after surgery. SAMPLE POPULATION 16 forelimbs obtained from 8 mature horses. PROCEDURE Tenotomy and subsequent tenorrhaphy was performed in anesthetized horses. Following systemic administration of heparin, horses were euthanatized and the limbs were removed and placed under tension to load the flexor tendons. The intrinsic vasculature was then perfused with a mixture of barium sulfate and water. Four-millimeter sections of the SDFT were prepared for microangiographic analysis. Mean vessel density was calculated for each section by use of a grid consisting of 1.5-mm2 vascular assessment squares (VAS). Comparisons were made among the control, LLP and 3LP groups. RESULTS Mean +/- SD vessel density was 3.11 +/- 0.38, 1.47 +/- 0.47, and 2.01 +/- 0.63 perfused vessels/1.5 mm2 for control, LLP and 3LP groups, respectively. Significant differences in vascular density were detected between the control and 3LP groups, control and LLP groups, and LLP and 3LP groups. CONCLUSIONS AND CLINICAL RELEVANCE Use of the LLP and 3LP pattern has deleterious effects in vitro on the intrinsic vasculature of the SDFT. However, the 3LP pattern was less disruptive to the intrinsic vasculature, compared with the effects for the LLP. Use of the 3LP tenorrhaphy suture pattern in clinical situations may result in less damage to the intrinsic vasculature of the SDFT of horses during convalescence.
Evaluation of a Comprehensive, Adaptable, Life- Affirming, Longitudinal (CALL) palliative care project.

BACKGROUND Many persons with life-threatening illnesses require services before they begin receiving hospice or hospital-based palliative care. Although health care professionals focus on diagnosis, treatment, and comfort care, patients and families view the illness as it impacts their whole life. OBJECTIVE Evaluating a Comprehensive, Adaptable, Life-Affirming, Longitudinal (CALL) intervention to patients with life-threatening cancer, cardiac illness, respiratory conditions or dementia. The CALL Care interventions are consistent with the 2004 Clinical Practice Guidelines for Quality Palliative Care. DESIGN Prospective observational study. SETTING AND SUBJECTS Eleven sites (1 primary care, 8 acute care, and 2 long term care) located around the United States. Two hundred ninety-five patients and families enrolled in the study; many were enrolled for over 12 months. MEASUREMENTS Patient self-reported Modified City of Hope Questionnaire addressing physical, psychosocial, emotional, and spiritual status and health care experiences; completed at enrollment and every three months. Family members completed Modified City of Hope Bereaved Family Questionnaire, with contents similar to the patient questionnaire. Sites compiled health care and community services utilization data regularly. RESULTS Participants reported improved pain and symptom management. Fewer than one third were hospitalized (29%), and 7% had a critical care admission while enrolled. In addition, participants had high use of hospice (48% of those who died), and were likely to die at home (38% of those who died). CONCLUSIONS CALL Care is an effective interdisciplinary intervention approach to individualize care for people living with life-threatening illness.
The Effects of Music Therapy in Cardiac Healthcare

Music therapy has been applied with patients who present with acute myocardial infarction, coronary artery bypass graft, and other cardiac conditions. Music therapists integrate their techniques into cardiac care units, often treating patients who require assistance in coping with stress. This article reviews the research supporting the use of music as therapy and offers case examples to illustrate a variety of clinical methods.