Miracle-dz/UniFAR-FTUs huggingface dataset:
Damage Diagnosis in 3D Structures Using a Novel Hybrid Multiobjective Optimization and FE Model Updating Framework

Structural damage detection is a well-known engineering inverse problem in which the extracting of damage information from the dynamic responses of the structure is considered a complex problem. Within that area, the damage tracking in 3D structures is evaluated as a more complex and difficult task. Swarm intelligence and evolutionary algorithms (EAs) can be well adapted for solving the problem. For this purpose, a hybrid elitist-guided search combining a multiobjective particle swarm optimization (MOPSO), Lévy flights (LFs), and the technique for the order of preference by similarity to ideal solution (TOPSIS) is evolved in this work. Modal characteristics are employed to develop the objective function by considering two subobjectives, namely, modal strain energy (MSTE) and mode shape (MS) subobjectives. The proposed framework is tested using a well-known benchmark model. The overall strong performance of the suggested method is maintained even under noisy conditions and in the case of incomplete mode shapes.
The Efficacy and Safety of Once-daily Fluticasone Furoate/Umeclidinium/Vilanterol Versus Twice-daily Budesonide/Formoterol in a Subgroup of Patients from China with Symptomatic COPD at Risk of Exacerbations (FULFIL Trial)

Abstract The FULFIL study evaluated once-daily fluticasone furoate/umeclidinium/vilanterol (FF/UMEC/VI) 100 µg/62.5 µg/25 µg versus twice-daily budesonide/formoterol (BUD/FOR) 400 µg/12 µg in patients with symptomatic COPD at risk of exacerbations. FULFIL demonstrated clinically meaningful and statistically significant improvements at Week 24 in trough forced expiratory volume in 1 second (FEV1), St George’s Respiratory Questionnaire (SGRQ) Total scores and reduced exacerbation frequency. Predefined analyses were performed to evaluate treatment effects in a subgroup of patients recruited in China (China subgroup; FF/UMEC/VI, n = 32; BUD/FOR, n = 29). Analyses included treatment by region (China versus non-China) to allow estimated treatment effects in patients from China to be compared with those of the non-China subgroup and the overall FULFIL intent-to-treat (ITT) population. In the China subgroup at Week 24: the mean change from baseline in trough FEV1 was 125 mL (95% confidence interval [CI] 36, 214) for FF/UMEC/VI and -70 mL (95% CI -163, 23) BUD/FOR (between-treatment difference: 195 mL [95% CI 67, 323]; p = 0.003) and in SGRQ Total score was -5.6 units (95% CI -10.5, -0.7) and -0.3 units (95% CI -5.4, 4.7), respectively (between-treatment difference: -5.3 [95% CI -12.3, 1.7]; p = 0.140). Fewer moderate/severe exacerbations occurred with FF/UMEC/VI than BUD/FOR (16% and 28%, respectively). The overall incidence of adverse events was similar between arms (FF/UMEC/VI: 38%; BUD/FOR: 31%). This prespecified subgroup analysis of patients recruited in China to FULFIL demonstrated comparable efficacy and safety to that observed in the non-China and in the overall ITT populations, for FF/UMEC/VI versus BUD/FOR.
Novel silk fibroin nanoparticles incorporated silk fibroin hydrogel for inhibition of cancer stem cells and tumor growth

Background A multi-drug delivery platform is needed as the intra-tumoral heterogeneity of cancer leads to different drug susceptibility. Cancer stem cells (CSCs), a small population of tumor cells responsible for tumor seeding and recurrence, are considered chemotherapy-resistant and have been reported to be sensitive to salinomycin (Sal) instead of paclitaxel (Ptx). Here we report a novel silk fibroin (SF) hydrogel-loading Sal and Ptx by incorporating drug-loaded silk fibroin nanoparticles (SF-NPs) to simultaneously kill CSCs and non-CSCs. Methods Using the method we have previously reported to prepare Ptx-loaded SF-NPs (Ptx-SF-NPs), Sal-loaded SF-NPs (Sal-SF-NPs) were fabricated under mild and non-toxic conditions. The drug-loaded SF-NPs were dispersed in the ultrasound processed SF solution prior to gelation. Results The resulting SF hydrogel (Sal-Ptx-NP-Gel) retained its injectable properties, exhibited bio-degradability and demonstrated homogeneous drug distribution compared to the non-NP incorporated hydrogel. Sal-Ptx-NP-Gel showed superior inhibition of tumor growth compared to single drug-loaded hydrogel and systemic dual drug administration in the murine hepatic carcinoma H22 subcutaneous tumor model. Sal-Ptx-NP-Gel also significantly reduced CD44+CD133+ tumor cells and demonstrated the least tumor formation in the in vivo tumor seeding experiment, indicating superior inhibition of cancer stem cells. Conclusion These results suggest that SF-NPs incorporated SF hydrogel is a promising drug delivery platform, and Sal-Ptx-NP-Gel could be a novel and powerful locoregional tumor treatment regimen in the future.
Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection

GATA transcription factors play a crucial role in the regulation of immune functions across metazoans. In Caenorhabditis elegans, the GATA transcription factor ELT-2 is involved in the control of not only infections but also recovery after an infection. We identified RPT-6, part of the 19S proteasome subunit, as an ELT-2 binding partner that is required for the proper expression of genes required for both immunity against bacterial infections and recovery after infection. We found that the intact ATPase domain of RPT-6 is required for the interaction and that inhibition of rpt-6 affected the expression of ELT-2-controlled genes, preventing the appropriate immune response against Pseudomonas aeruginosa and recovery from infection by the pathogen. Further studies indicated that SKN-1, which is an Nrf transcription factor involved in the response to oxidative stress and infection, is activated by inhibition of rpt-6. Our results indicate that RPT-6 interacts with ELT-2 in vivo to control the expression of immune genes in a manner that is likely independent of the proteolytic activity of the proteasome.
Epidermal growth factor receptor gene mutations in non-small-cell lung cancer cells are associated with increased radiosensitivity in vitro

Introduction There is still lack of specific biomarkers in predicting the radiosensitivity of non-small cell lung cancer (NSCLC) patients in clinic. Previous studies have shown that the EGFR gene status may correlate with radiosensitivity of NSCLC. However, the underlying mechanisms remain unknown. The aim of this study was to further investigate the correlation between EGFR mutation status and the NSCLC cell radiosensitivity and to explore the possible cellular mechanism. Methods Eight NSCLC cell lines with different EGFR gene status were irradiated by linear accelerator, and the radiosensitivity between the cell lines was compared by cell colony formation assay and cell proliferation assay. Cell cycle and apoptosis were analysed by flow cytometry. Radiosensitivity-related protein expression was detected by Western blotting. Results In the present study, we found that NSCLC cell lines with the epidermal growth factor receptor (EGFR) gene mutations were more sensitive to X-ray irradiation than those with wild-type EGFR (P<0.05). No difference in radiosensitivity was observed between NSCLC cells with EGFR exon19 deletion (Del 19) mutation and exon 21 point mutation at position 858 (L858R) with or without T790M mutation (P<0.05), as well as between NSCLC cells with EGFR mutation and those with acquired EGFR-tyrosine kinase inhibitors (TKIs) resistance. Mechanistically, EGFR mutations promoted NSCLC cell apoptosis in response to X-ray irradiation through the upregulation of proapoptotic protein Bax and downregulation of anti-apoptotic proteins such as Bcl-2 and DNA-dependent protein kinase catalytic subunit. In addition, phosphorylated histone (γ-H2AX) foci assay showed that EGFR mutations sustained irradiation-induced DNA damage. Conclusion Taken together, our study demonstrates that EGFR mutations are closely associated with the increased sensitivity of NSCLC cell lines to X-ray irradiation and that EGFR mutation status is a potentially useful indicator to evaluate the effectiveness of radiotherapy in the treatment of NSCLC.
Extending the predictive capabilities of hand-oriented behavioural biometric systems

The discipline of biometrics may be broadly defined as the study of using metrics related to human characteristics as a basis for individual identification and authentication, and many approaches have been implemented in recent years for many different scenarios. A sub-section of biometrics, specifically known as soft biometrics, has also been developing rapidly, which focuses on the additional use of information which is characteristic of a user but not unique to one person, examples including subject age or gender. Other than its established value in identification and authentication tasks, such useful user information can also be predicted within soft biometrics modalities. Furthermore, some most recent investigations have demonstrated a demand for utilising these biometric modalities to extract even higher-level user information, such as a subject\textsc{\char13}s mental or emotional state. The study reported in this thesis will focus on investigating two soft biometrics modalities, namely keystroke dynamics and handwriting biometrics (both examples of hand-based biometrics, but with differing characteristics). The study primarily investigates the extent to which these modalities can be used to predict human emotions. A rigorously designed data capture protocol is described and a large and entirely new database is thereby collected, significantly expanding the scale of the databases available for this type of study compared to those reported in the literature. A systematic study of the predictive performance achievable using the data acquired is presented. The core analysis of this study, which is to further explore of the predictive capability of both handwriting and keystroke data, confirm that both modalities have the capability for predicting higher level mental states of individuals. This study also presents the implementation of detailed experiments to investigate in detail some key issues (such as amount of data available, availability of different feature types, and the way ground truth labelling is established) which can enhance the robustness of this higher level state prediction technique.
Protein lysine acetylation plays a regulatory role in Bacillus subtilis multicellularity

Protein lysine acetylation is a post-translational modification that alters the charge, conformation, and stability of proteins. A number of genome-wide characterizations of lysine-acetylated proteins, or acetylomes, in bacteria have demonstrated that lysine acetylation occurs on proteins with a wide diversity of functions, including central metabolism, transcription, chemotaxis, and cell size regulation. Bacillus subtilis is a model organism for studies of sporulation, motility, cell signaling, and multicellular development (or biofilm formation). In this work, we investigated the role of global protein lysine acetylation in multicellular development in B. subtilis. We analyzed the B. subtilis acetylome under biofilm-inducing conditions and identified acetylated proteins involved in multicellularity, specifically, swarming and biofilm formation. We constructed various single and double mutants of genes known to encode enzymes involved in global protein lysine acetylation in B. subtilis. Some of those mutants showed a defect in swarming motility while others demonstrated altered biofilm phenotypes. Lastly, we picked two acetylated proteins known to be important for biofilm formation, YmcA (also known as RicA), a regulatory protein critical for biofilm induction, and GtaB, an UTP-glucose-1-phosphate uridylyltransferase that synthesizes a nucleotide sugar precursor for biosynthesis of exopolysaccharide, a key biofilm matrix component. We performed site-directed mutagenesis on the acetylated lysine codons in ymcA and gtaB, respectively, and assayed cells bearing those point mutants for biofilm formation. The mutant alleles of ymcA(K64R), gtaB(K89R), and gtaB(K191R) all demonstrated a severe biofilm defect. These results indicate the importance of acetylated lysine residues in both YmcA and GtaB. In summary, we propose that protein lysine acetylation plays a global regulatory role in B. subtilis multicellularity.
A case report of an adult male patient with Avoidant/Restrictive Food Intake Disorder treated with CBT.

Avoidant/Restrictive Food Intake Disorder (ARFID) is a new diagnosis within the Feeding and Eating Disorders in DSM-5 (American Psychiatric Association 2013). It has replaced the Feeding Disorder of Infancy and Early Childhood (FDIEC) included in the DSM-IVTR. ARFID diagnosis is intended to gather individuals who meet criteria for the previous FDIEC, but also to collect other patients with clinically significant eating problems who were formerly defined as Eating Disorder Not Otherwise Specified (EDNOS). ARFID symptoms typically become evident in infancy or childhood, but they may also present or persist during adulthood. People who fulfill the criteria have developed some kind of problem with eating (or for very young children, a problem with feeding) or food. As a result, the person is not able to take the enough nutrition or energy intake through diet and may end up losing weight, so some patients might need supplements to get adequate feeding. Younger kids might not gain weight or grow as expected (Kohn 2016). Different types of eating behaviors might warrant an ARFID diagnosis, e.g. avoiding foods on the base of sensory characteristics like color or texture, difficulty digesting certain foods, eating only very small portions, having no appetite or lacking interest in food or eating, or being afraid to eat after a frightening episode of choking or vomiting. Many individuals with ARFID may have problems at school or work because of their eating struggles such as avoiding work lunches, not getting schoolwork done because of the time it takes them to eat, or even avoiding meeting friends or family during social events where food is present. One of the clinical features of ARFID as described in DSM-5 (i.e. concern about aversive consequences of eating) may overlap with Orthorexia Nervosa (ON) (Dunn & Bratman 2015). ON is characterized by the obsessive focus on "healthy" eating, as defined by a dietary theory or set of beliefs whose specific details may vary and it is marked by exaggerated emotional distress related to food choices perceived as unhealthy. There are few studies in the literature about ARFID and most of them have been done with children and adolescents (Mammel & Ornstein 2015, Norris et al. 2014). Besides, recently, we found studies that describe this disorder in adult patients, predominantly women (Nakai et al. 2016, 2017). Instead we present the case of an adult male ARFID from the first psychiatric evaluation until a follow-up of 6 months after treatment. This case report aims to describe if a Cognitive Behavioral Therapy (CBT) approach with the family involvement is effective in the treatment of ARFID in adulthood.
The effect of small group teaching on quality of life in pregnant women with nausea and vomiting: A clinical trial

INTRODUCTION: Nausea and vomiting in pregnancy is the most common medical complication in the first trimester of pregnancy. That associated with a wide range of physical and mental symptoms for the patient and his family and can affect the quality of life of women's life. In addition, the training to improve knowledge, in adjusting diet and lifestyle, and leading to decrease nausea and vomiting. Due to the positive characteristics of training using the small groups, this study was applied with the objective of “The effect of small group teaching on quality of life in pregnant women with nausea and vomiting. ” METHODS: This study was applied based on intervention, on 59 nulliparous women with nausea and vomiting who referred to obstetrics ward of selected health and treatment centers in Neishaboor County. The training was presented by a researcher in group intervention in 8 sessions, each 45–30 min in small groups (3 groups 10 members). The control group received routine care. The study instruments (nausea and vomiting of pregnancy quality of life questionnaire) and (modified-PUGE) form. The results were analyzed using the Wilcoxon test, Mann–Whitney, and t-test. RESULTS: Research units were no statistically significant difference of personal data, at the beginning of the study, and before intervention, was not differences between the mean change scores of postpartum stress disorder in two groups (P = 0/192). While the mean quality of life score in the intervention and control groups was statistically significant difference after intervention (P = 0.001). Quality of life score in the intervention group had statistically significant difference before and after the intervention (P = 0.001). CONCLUSION: Training in the intervention group with small group strategies has more effective in comparison to conventional training of nausea and vomiting.
HBV-HCV Coinfection: Viral Interactions, Management, and Viral Reactivation

Abstract Hepatitis B virus (HBV) and hepatitis C virus (HCV) coinfection is a complex clinical entity that has an estimated worldwide prevalence of 1–15%. Most clinical studies have shown that progression of disease is faster in HBV-HCV coinfected patients compared to those with monoinfection. Hepatocellular carcinoma development appears to have higher rate in coinfections. Viral replication in coinfected cells is characterized by a dominance of HCV over HBV replication. There are no established guidelines for treatment of HBV-HCV coinfection. Studies on interferon-based therapies and direct-acting antivirals have shown varying levels of efficacy. Clinical reports have indicated that treatment of HCV without suppression of HBV increases the risk for HBV reactivation. In this review, we appraise studies on both direct-acting antivirals and interferon-based therapies to evaluate the efficacy and rates of reactivation with each regimen. Screening for and prevention of coinfection are important to prevent serious HBV reactivations.
Evaluation of Correlations Between Food-Specific Antibodies and Clinical Aspects of Hashimoto’s Thyroiditis

Abstract Objective: We have comprehensively evaluated an immunologic response to food antigens, mediated by immunoglobulin G (IgG) antibodies, on clinical aspects of Hashimoto’s thyroiditis (HT). Methods: IgG antibodies to 125 food antigens were measured in serum samples of 74 HT patients and 245 controls using microarray-based enzyme-linked immunosorbent assay (ELISA) test. We analyzed differences in IgG levels between two groups and evaluated correlations between food-specific IgG levels and HT-related clinical phenotypes (thyroid hormones/antibodies, symptoms of hypothyroidism, measures of body size and blood pressure) and food consumption in HT patients. Results: We observed increased IgG levels to 12 different food antigens in either HT cases or controls, of which plum-specific IgG antibodies were significantly higher (p = 1.70 × 10−8), and almond-specific IgG antibodies were significantly lower (p = 8.11 × 10−5) in HT patients in comparison to controls, suggesting their possible roles in HT etiology or symptomatology. There was no significant correlation between any of 12 increased food-specific IgG antibodies, along with gluten-specific IgG, with clinically important phenotypes, such as thyroid hormones/antibodies or symptoms. Among other tested correlations, the most interesting is the negative correlation between coffee and tea combined IgG levels and number of symptoms, suggesting possible beneficial effect of tea and coffee on disease symptoms. We also found that food consumption is not correlated with IgG levels. Conclusions: Distribution of food-specific IgG antibodies is comparable between HT patients and controls, with the exception of plum and almond. There is no evidence that increased food-specific IgG antibodies are associated with clinical aspects of HT. Clarification of biology behind formation of these antibodies is needed.
Nothing personal: algorithmic individuation on music streaming platforms

Raymond Williams once wrote, ‘… there are in fact no masses, but only ways of seeing people as masses’. In an age of personalized media, the word ‘masses’ seems like an anachronism. Nevertheless, if Williams were to study contemporary online platforms, he would no doubt conclude that there are in fact no individuals, but only ways of seeing people as individuals. This article explores this idea by taking a closer look at online music streaming services. It first conducts a comparison of how two leading streaming platforms conceive of the individual music listener. Then, drawing from Gilbert Simondon’s theory of individuation, it demonstrates how ways of seeing the individual work to enact the individual on these platforms. In particular, ways of seeing are heavily influenced by the consumer categories that are defined and demanded by advertisers. This article concludes with an examination of how commercial imperatives shape ‘ways of seeing’ and ‘algorithmic individuation’ on music streaming platforms.
c-MYC Drives Breast Cancer Metastasis to the Brain, but Promotes Synthetic Lethality with TRAIL

Brain metastasis in breast cancer is particularly deadly, but effective treatments remain out of reach due to insufficient information about the mechanisms underlying brain metastasis and the potential vulnerabilities of brain-metastatic breast cancer cells. Here, human breast cancer cells and their brain-metastatic derivatives (BrMs) were used to investigate synthetic lethal interactions in BrMs. First, it was demonstrated that c-MYC activity is increased in BrMs and is required for their brain-metastatic ability in a mouse xenograft model. Specifically, c-MYC enhanced brain metastasis by facilitating the following processes within the brain microenvironment: (i) invasive growth of BrMs, (ii) macrophage infiltration, and (iii) GAP junction formation between BrMs and astrocytes by upregulating connexin 43 (GJA1/Cx43). Furthermore, RNA-sequencing (RNA-seq) analysis uncovered a set of c-MYC–regulated genes whose expression is associated with higher risk for brain metastasis in breast cancer patients. Paradoxically, however, increased c-MYC activity in BrMs rendered them more susceptible to TRAIL (TNF-related apoptosis-inducing ligand)–induced apoptosis. In summary, these data not only reveal the brain metastasis-promoting role of c-MYC and a subsequent synthetic lethality with TRAIL, but also delineate the underlying mechanism. This suggests TRAIL-based approaches as potential therapeutic options for brain-metastatic breast cancer. Implications: This study discovers a paradoxical role of c-MYC in promoting metastasis to the brain and in rendering brain-metastatic cells more susceptible to TRAIL, which suggests the existence of an Achilles' heel, thus providing a new therapeutic opportunity for breast cancer patients.
Accurate timetrees require accurate calibrations

Morris et al. (1) estimate divergence times for land plants (embryophytes), concluding that they originated in the early Phanerozoic (515 to 473 Ma; midpoint, 494 Ma). In contrast, other molecular clock studies have placed that event 40% earlier, in the Precambrian (707 to 670 Ma) (2⇓–4). Knowing the correct time bears on understanding how land plants have impacted the biosphere (2). Morris et al. (1) conclude that the tree topology and size of the dataset had little impact on their results. They also suggest that their results were robust to “dating strategies,” which included removing a single maximum calibration while keeping all other maximum and minimum calibrations. The 37 minimum … [↵][1]1To whom correspondence should be addressed. Email: sbh{at}temple.edu. [1]: #xref-corresp-1-1