Miracle-dz/UniFAR-FTUs huggingface dataset:
Family of Ebf/Olf‐1‐related genes potentially involved in neuronal differentiation and regional specification in the central nervous system

Two novel mouse genes, Ebf2 and Ebf3, have been identified which show high similarity to the rodent Ebf/Olf‐1 and the Drosophila collier genes. The strong conservation of the protein regions corresponding to the DNA binding and dimerisation domains previously defined in Ebf strongly suggests that Ebf2 and Ebf3 also constitute DNA sequence‐specific transcription factors. Determination of the chromosomal locations of the two genes indicated that the different members of this novel mouse multigene family are not clustered. A detailed analysis of the expression of each of the three Ebf genes in the developing central nervous system revealed partially overlapping patterns with two salient features: 1) In the region extending from the midbrain to the spinal cord, the expression of the three genes correlated with neuronal maturation, with a general activation in early post‐mitotic cells, followed by specific patterns of extinction also consistent with the neurogenic gradient. 2) In the forebrain area, although the patterns of expression of the Ebf genes also reflected neuronal maturation, they appeared in addition to be region specific. These data suggest that Ebf genes may be involved in the control of neuronal differentiation in the CNS and in enforcing regional diversity in populations of post‐mitotic forebrain neurons. Dev. Dyn. 1997;210:191–205. © 1997 Wiley‐Liss, Inc.
Discharge Teaching About Warfarin: Patient Retention of Knowledge

For patients who are posthospitalization and on warfarin therapy, one key component is the proper management of warfarin therapy. This descriptive study was conducted using a telephone survey to determine what level of knowledge exists regarding management of warfarin among patients back at home. Analysis of data collection results provides recommendations for evidenced-based practices for the home health clinician, which may improve patient outcomes. Basic knowledge of food and medication interactions can make a significant impact on reduction of adverse events of bleeding or clot formation. An understanding of the therapeutic level of warfarin obtained from lab tests could be of greater value.
One-Dimensional Bargaining with a General Voting Rule

We study a model of multilateral bargaining over social outcomes represented by points in the unit interval. The acceptance or rejection of a proposal is determined by an acceptance rule as represented by the collection of decisive coalitions. The focus of the paper is on the asymptotic behavior of subgame perfect equilibria in stationary strategies as the discount factor goes to one. We show that, along any sequence of stationary subgame perfect equilibria the social acceptance set collapses to a point. This point, called the limit of bargaining equilibria, is independent of the sequence of equilibria and is uniquely determined by the set of players, the utility functions, the recognition probabilities, and the acceptance rule. The central result of the paper is a characterization of the limit of bargaining equilibria as a unique zero of the characteristic equation.
Dietary patterns and upper aerodigestive tract cancers: an overview and review.

BACKGROUND The relationship between diet and cancers of the upper aerodigestive tract (UADT) has been investigated through dietary patterns. DESIGN Published studies on the relationship between a priori and a posteriori dietary patterns and UADT cancers were selected through a Medline search. RESULTS Twenty-four case-control studies were identified. Most of them identified a posteriori dietary patterns, mainly using principal component factor analysis, and a few used a priori dietary patterns, based on the available evidence on known effects of dietary habits on UADT cancers. In one study, no association was found between the identified patterns and UADT cancers. All the remaining 23 papers reported at least one favorable or unfavorable dietary pattern related to UADT cancers. The most consistent findings are the beneficial role of a dietary pattern based on fruit and vegetables or nutrients mostly contained in such foods, and the unfavorable role of an alcohol drinker pattern. A possible unfavorable role of patterns based on meats and animal products emerged as well. CONCLUSION The consistency of results among populations indicates that diets rich in fruit and vegetables, and poor in alcohol and animal products are favorable for UADT cancers.
Crianças usuárias de implante coclear: desempenho acadêmico, expectativas dos pais e dos professores

Um dos recursos tecnologicos atualmente empregados na (re)habilitacao de criancas com deficiencia auditiva e o implante coclear multicanal (I.C). Estudos de seguimento tem se realizado, buscando avaliar quais os resultados conquistados pelas criancas usuarias de implante coclear em relacao as habilidades de audicao e de linguagem oral. Contudo, quanto ao desempenho academico destas criancas sao escassos os trabalhos desenvolvidos, especialmente no Brasil. Os objetivos deste estudo foram, portanto, descrever o desempenho academico de criancas implantadas com idades entre 6 a 12 anos e fazer a analise de associacoes dos resultados do desempenho academico com as seguintes variaveis independentes: idade, serie, limiar de deteccao de voz, tempo de uso do IC, tempo de privacao sensorial, maior nivel de escolaridade da familia. Alem destes objetivos tambem competiu ao estudo analisar as informacoes e expectativas de pais e professores a respeito do desempenho academico destas criancas. Para a consecucao de tais objetivos, realizou-se coleta e analise de dados dos prontuarios das criancas, avaliacoes individuais do desempenho academico por meio de testes padronizados e concomitantemente, questionarios foram realizados com os pais e professores das criancas participantes. Os dados foram analisados quantitativamente e qualitativamente e foi aplicado o tratamento estatistico pertinente. Concluiu-se que as criancas usuarias de I.C. apresentaram um desempenho nos testes abaixo do esperado para sua idade e serie e que houve associacao estatisticamente significante dos escores do teste TDE com a variavel serie. Quanto as informacoes dos pais das criancas participantes observou-se que as principais preocupacoes destes referiram-se a aprendizagem da leitura e escrita, quanto ao acompanhamento das atividades de sala de aula como os colegas ouvintes e preocupacoes quanto a quebra do dispositivo(IC). Em relacao as informacoes e expectativas dos professores, as conclusoes foram que os professores tem poucas informacoes a respeito do implante coclear, expectativas na media ou abaixo da media quanto ao desempenho academico do aluno implantado, mas expectativas positivas em relacao ao potencial de aprendizado das criancas implantadas, especialmente as mais jovens.
Arterial Embolisms and Thrombosis in Upper Extremity Ischemia

Objective: Upper extremity ischemia (UEI) is an uncommon condition that can lead to permanent disability. There is a limited understanding of the etiology, management, and outcomes of the disease. Methods: We retrospectively reviewed the charts of all patients who were diagnosed with “embolism and/or thrombosis of arteries of upper extremity” at our institution from January 2005 to December 2013. Results: Patients diagnosed with embolisms were older (P < .001), more likely to undergo thromboembolectomy (P < .001), had higher rates of hypertension (P = .001), and had longer lengths of hospital stay (P = .002). There were no significant differences in complications or mortality at 30 days and up to 1 year. Conclusion: At our center, embolism was found to be the most common etiology for UEI followed by thrombosis and stenosis. Patients presented with embolism were older, were more likely to undergo thromboembolectomy, and had higher rates of hypertension and longer hospital stays.
Nonresidential father involvement: a test of a mid-range theory.

This study tested a theoretical model of postdivorce involvement of nonresidential fathers with their children. The hypotheses were (a) that postdivorce father involvement is related to father parenting role identity, role clarity, child relationship quality, and father role hierarchy ranking; and (b) that several variables serve as moderators for the relationship between father parenting role identity and father involvement. Data were collected via self-report questionnaires administered to 101 fathers. Path analytic techniques were used to assess the theoretical model. Findings supported the hypotheses related to the core constructs within the theory, but not for the moderator variables within the study. Role clarity and child relationship quality were found to have both direct and indirect effects on father involvement. Father parenting role identity had a direct effect on father involvement but also functioned as a meditating variable for constructs within the model. Significant paths were found for two variables, joint custody and satisfaction with the legal system. Father postdivorce involvement is a complex phenomenon, and forces external to fathers can contribute to levels of postdivorce involvement (e.g., legal system issues).
Protein Expression Patterns in ovarian cancer cells Associated with Monofunctional Platinums Treatment

Platinum drugs cisplatin and carboplatin, given in combination with paclitaxel, constitute the standard chemotherapy against ovarian cancer (OC). Oc chemoresistance is a major obstacle to effective treatment, but knowledge of the mechanisms that underlie it remains incomplete. We thus sought to discover key proteins associated with platinum resistance by comparing A2780 OC cells with A2780cisR cells (resistant cells derived from the A2780 line) to identify proteins with markedly altered expression levels in the resistant cells. We also determined which proteins in these cells had altered expression in response to treatment with either designed monofunctional platinum alone or a combination with cisplatin with selected phytochemical therapeutic agents. We thus performed proteomic analysis using 2D-gel electrophoresis A2780 and A2780cisR to identify proteins with differential expression; these were eluted and analysed by mass spectrometry to identify them. A total of 122 proteins were found to be differentially expressed between A2780 and A2780cisR cell lines in the absence of any drug treatment. Among them, levels of 27 proteins in A2780cisR cell line were further altered (up-or down-regulated) in response to one or more of the drug treatments. We then investigated primary OC tissue RNA expression levels (compared to l ovarian tissue) of genes coding for these candidate 27 proteins using publically available datasets (The Cancer Genome Atlas). We assessed how expression of these genes in OC tissue associates with patient survival using Cox Proportional Hazard (PH) regression models to determine relative risk of death associated with each factor. Our Cox PH regression-based machine learning method confirmed a significant relationship of mortality with altered expression of ARHGDIA, CCT6A and HISTIH4F genes. This indicated that these genes affect OC patient survival, i.e., provided mechanistic evidence, in addition to that of the clinical traits, that these genes may be critical mediators of the processes that underlie OC progression and mortality. Thus, we identified differentially expressed proteins that are implicated in platinum-based chemotherapy resistance mechanisms which may serve as resistance biomarkers. These drug resistance associated proteins may also serve as potential OC therapeutic targets whose blockade may enhance the effectiveness of platinum based drugs.
Evaluating Brassica species as an alternative control measure for root-knot nematode ( M . incognita ) in Georgia vegetable plasticulture

Multiple Brassica species commonly grown in Georgia were evaluated as a potential alternative to methyl bromide for management of root-knot nematode in vegetables. Brassica species produce general biocides called glucosinolates and were grown as cover crops and incorporated as green manures prior to transplanting of vegetable crop. Nematicidal activity of the Brassica species was based on net changes in nematode population and root damage caused by root-knot infection and feeding. Plant growth and crop yield was also evaluated to determine the response of the subsequent vegetable crop to the incorporated Brassica species. Incorporation of select Brassica species reduced root-knot populations and root damage caused by root-knot infection comparable to non-Brassica species cover crops with metam sodium in most years; however, the level and consistency of the nematicidal activity varied between and within Brassica species. Variations in plant growth and yield were also observed among and with Brassica and non-Brassica species. Generally, increased growth and yield corresponded with cover crop treatments that had the lowest levels of root-knot nematode populations at planting of the subsequent crop. Several cover crop treatments had a negative affect on the growth and yield potential of the vegetable crops. Therefore, a producer will need to choose a Brassica species cover crop that can significantly reduce nematode populations without adversely affecting the growth/yield of the subsequent vegetable crop. Published by Elsevier Ltd.
The value of procalcitonin as an infection marker in cardiac surgery.

BACKGROUND/OBJECTIVE Cardiopulmonary bypass generally leads to an unspecific increase of inflammatory parameters after cardiac operations. Increased Procalcitonin (PCT)-levels in serum, particularly after contamination with bacterial endotoxines, can be used as a marker for specific infections. The objective of this prospective study was to evaluate the course of PCT after cardiac surgery for the differential diagnosis of infections/unspecific inflammatory reactions, compared to routine infection parameters. METHOD Serum PCT levels were measured in 400 routine cardiosurgical patients preoperatively and at 1. , 2. , 4. and 6. postoperative days with a luminescence immunoassay. PCT-values were compared to the patient's clinical infection status, body temperature, leukocyte count and C-reactive protein (CRP). RESULTS 364 patients had an infection-free postoperative course, 27 patients developed infections. All of these patients showed elevated infection parameters at 1-2. postoperative days. In patients without infection, these parameters decreased after 2. postoperative day. Patients predisposed to an infection had continuously high temperature, leukocytes, CRP and PCT until 4.postoperative day with leukocytes and CRP decreasing after 4.postoperative day. PCT however showed a divergent course with a second increase in these patients between 4. -6. postop day (p<0.001). At this time, no clinical sign of an infection was evident. The increase of PCT was independent of infection type, but most apparent in bacteriemia/sepsis. CONCLUSION Based on its different course from other parameters in infection development between 4. -6. days, PCT can probably be used as a predictive marker in bacterial infections after cardiac surgery. The cost of the used immunoassay however will set the limits for a routine application.
Prominin: A Story of Cholesterol, Plasma Membrane Protrusions and Human Pathology

Prominin is the first identified member of a novel family of polytopic membrane proteins conserved throughout the animal kingdom. It has an unusual membrane topology, containing five transmembrane domains and two large glycosylated extracellular loops. In mammals, prominin is expressed in various embryonic and adult epithelial cells, as well as in nonepithelial cells, such as hematopoietic stem cells. At the subcellular level, prominin is selectively localized in microvilli and other plasma membrane protrusions, irrespective of cell type. At the molecular level, prominin specifically interacts with membrane cholesterol and is a marker of a novel type of cholesterol‐based lipid ‘raft’. A frameshift mutation in the human prominin gene, which results in a truncated protein that is no longer transported to the cell surface, is associated with retinal degeneration. Given that prominin is concentrated in the plasma membrane evaginations at the base of the outer segment of rod photoreceptor cells, which are essential precursor structures in the biogenesis of photoreceptive disks, it is proposed that prominin has a role in the generation of plasma membrane protrusions, their lipid composition and organization and their membrane‐to‐membrane interactions.
One-Carbon Metabolism, Spermatogenesis, and Male Infertility

Balanced diet is the natural source of micronutrients, such as folate and vitamins, vital for proper functioning of the body. One-carbon metabolic pathway along with folate and other vitamins plays an important role in DNA synthesis and in the establishment of epigenetic modifications like DNA/histone methylation. Spermatogenesis involves distinct cellular, genetic, and chromatin changes during the course of production of male gamete sperm. Folate and normal activity of 1-carbon metabolic pathway enzymes are central to nucleotide synthesis, methylation, and maintenance of genomic integrity as well as protection from DNA damage. As a result, polymorphisms in 1-carbon metabolic pathway genes affecting several physiological processes also have an impact on spermatogenesis and may affect directly or indirectly quality of sperm. Alterations in these processes may be a consequence of additive effect resulting from altered expression of 1-carbon metabolic pathway genes and/or inadequate folate/micronutrients supplementation. The present review provides an overview of different cellular and molecular events regulated by 1-carbon metabolic pathway enzymes and their impact on male reproductive health. It also summarizes the different studies where polymorphisms in the enzymes of 1-carbon metabolic pathway or folate deficiency are associated with male infertility and future prospects.
PCR BASED DETECTION OF GRAM NEGATIVE PSYCHROTROPHIC BACTERIA IN COWS RAW MILK

Investigations of the proteolytic Gram Negative Psychrotrophs(GNP) bacteria was the basal objective of this study. A conventional diagnostic PCR technique based on three pairs of primers including SerAgeneto amplify an 950 bp fragment of Acinetobacter sppDNA,serAgene for/A. hydrophila ( 650bp): and aprgene for/S. marcescens (500bps) was done.In the present study the 29 bacterial isolates obtained from 100 cows raw milk samples were collected randomly from healthy cows with different age and breed present in different farms of Thi-Qar province, previously refrigerated for 72 hr. These isolates subjected to cultural-based enrichment and PCRbased identification . The present results revealed that the raw milk GNP contamination overall ratio was 29% . Acinetobacter spp were the most predominant bacteria (16%) among the studied GNP contaminants, while A.hydrophila appeared in a ratio of 7% and S. marcescens showed the lower ratio ( 6%). ,the results of the studied genes product of GNP bacteria was considered to be highly statistically significant (P>0.001). The distribution of studied GNP according to age ,parturition number and breed of studied animals was investigated. The effect of these factors on the PCR-based identification results was considered to be not statistically significant(P>0.05) however, the higher overall ratio(29.1%) for cow raw milk contamination was observed in raw milk of cows between <3 < 9 year of age. In general cows at first age group (<3 < 9 year) showed high ratio of raw milk contamination(7.6 and6.3%) with GNP bacteria (Serratia marscense and Aremonase hydrophila respectively). Concerning the number of parturition, cows with high numbers of parturition( ˃ 6-<12) showed high overall ratio(38.5%) of contamination also high ratio of Acinetobacter spp(23.1%) ,Aremonase hydrophila(7.7%) and Serratia marscense Bas.J.Vet.Res.Vol.15,No.1,2016. ISI Impact Factor:3.461 162 (7.7%) were observed in the same group of cows. According to breed, a high overall ratio of GNP bacterial contamination was observed in 40% of crossbred cow raw milk followed by Friesian cows(32.1%). Beside that raw milk of crossbred cows showed a high ratio of contamination with Acinetobacter spp (20%) and Aremonase hydrophila(13.3%) while Serratia marscense appeared as a higher contaminant in Friesian cows raw milk with the ratio of its contamination was10.7%.
Title Socio-economic and psychological correlates of suicidality among Hong Kong working-age adults : Results from a population-based survey

Background. The global toll of suicide is estimated to be one million lives per year, which exceeded the number of deaths by homicide and war combined. A key step to suicide prevention is to prevent less serious suicidal behaviour to preclude more lethal outcomes. Although 61% of the world’s suicides take place in Asia and the suicide rates among middle age groups have been increasing since the economic crisis in many Asian countries, population-based studies of suicidal behaviour among working-age adults in non-western communities are scarce. Method. Data from a population-based survey with 2015 participants were used to estimate the prevalence of suicidal ideation and behaviour among the working-age population in Hong Kong, and to study the associated socio-economic and psychological correlates. We focused particularly on potential modulating factors between life-event-related factors and suicidal ideation. Results. Six per cent of the Hong Kong population aged 20–59 years considered suicide in the past year, while 1.4% attempted suicide. Hopelessness, reasons for living, and reluctance to seek help from family and friends had direct association with past-year suicidal ideation. Reasons for living were found to moderate the effect of perceived stress on suicidal ideation. Conclusions. Suicidality is a multi-faceted problem that calls for a multi-sectored, multi-layered approach to prevention. Prevention programmes can work on modulating factors such as reasons for living to reduce suicidal risk in working-age adults.
Long-Term Follow up of 610 Cases of Ménière's Disease

The aim of this paper is to discuss some of the statistical findings which have arisen from the study of the clinical records of patients suffering from Meniere's disease, in some of whom the follow up was over 10 years. The prognosis and treatment of unilateral Meniere's disease depend much on the statistical studies of several authors over the past three decades (Brain 1938, Cawthorne et al. 1942, Cawthome & Hewlett 1954, Harrison & Naftalin 1968). However, during the past three years, the findings of these workers have been seriously challenged by Stahle & Bergman (1967) and by Enander & Stahle (1967, 1969), whose figures
Bowel Habits of Normal Thai Children

Bowel habits were assessed in 400 healthy Thai children aged 1–72 months. The study was undertaken principally because information concerning the bowel habits of children living on a non-Western diet is lacking. Ninety-four percent of the children moved their bowels at least once a day. Mean frequency of bowel actions per day was maximal in the newborn period (3.3 stools) and declined to once a day from ages of 48 to 72 months. Most infants younger than 2 months old produced running stools; however, a change in stool consistency from runny to formed was evident in most children by 4 months of age. With increasing age, children produced larger stools: mean volume of stool was 16.6 ± 2.3 ml at 1 month of age and increased to 35.4 ± 7.6 ml at 48–72 months. In comparison with Western children, it seems that Thai children pass larger, softer, and more frequent stools. That difference in the bowel habits between the two ethnic groups may reflect the difference in the amount of natural fiber in their diets.