Miracle-dz/UniFAR-FTUs huggingface dataset:
Associations between Extracurricular Activity and Self-Regulation: A Longitudinal Study from 5 to 10 Years of Age

Purpose. Health promotion in youth is likely to benefit from enhancing academic achievement and physical activity. The present study examines how kindergarten childhood self-regulation skills and behaviors predict involvement in both structured and unstructured physical and nonphysical extracurricular activities in the fourth grade. As a second objective this study also investigated how kindergarten childhood participation in extracurricular activities predicts classroom engagement, reflective of self-regulation, by the fourth grade. Design. Secondary analyses were conducted using prospective-longitudinal data. Setting. The Quebec Longitudinal Study of Child Development, Quebec, Canada. Subjects. Participants were randomly selected at birth from a stratified sample of 2694 born in Québec, Canada, between 1997 and 1998. Participants were included if they had complete data on teacher ratings of child self-regulation as measured by classroom engagement and parent ratings of sports participation (n = 935). Measures. Teachers reported self-regulation skills in children through a measure of classroom engagement. Parents provided reports of child participation extracurricular activities. Analysis. Ordinary least-squares regressions were conducted. Results. A higher-frequency kindergarten involvement with structured physical activities was associated with fourth-grade classroom engagement (β = .061, 95% confidence interval [CI]: .017, .104). Better kindergarten classroom engagement predicted more frequent participation in fourth-grade structured physical activities (β = .799, 95% CI: .405, 1.192) and team sports (β = .408, 95% CI: .207, .608). Conclusion. Results suggest mutual relations between physical activity and self-regulation from kindergarten to grade four. This suggests strong learning skills indicative of self-regulation and opportunities to participate in supervised physical activities or sports teams may help children develop healthy dispositions and behaviors in emerging adolescence.
Immunohistochemical Analysis of ZnT1, 4, 5, 6, and 7 in the Mouse Gastrointestinal Tract

Expression of five zinc transporters (ZnT1, 4, 5, 6, and 7) of the Slc30 family in the mouse gastrointestinal tract was studied by immunohistochemical analysis. Results demonstrated unique expression patterns, levels, and cellular localization among ZnT proteins in the mouse gastrointestinal tract with some overlapping. ZnT1 was abundantly expressed in the epithelium of the esophagus, duodenum of the small intestine, and cecum of the large intestine. ZnT4 was predominantly detected in the large intestine. ZnT5 was mainly expressed in the parietal cell of the stomach and in the absorptive epithelium of the duodenum and jejunum. ZnT6 was predominantly detected in the chief cell of the stomach, columnar epithelial cells of the jejunum, cecum, colon, and rectum. Lastly, ZnT7 was observed in all epithelia of the mouse gastrointestinal tract with the highest expression in the small intestine. Expression of ZnT proteins in the absorptive epithelial cell of the gastrointestinal tract suggests that ZnT proteins may play important roles in zinc absorption and endogenous zinc secretion.
A circadian enhancer mediates PER-dependent mRNA cycling in Drosophila melanogaster

Genes expressed under circadian-clock control are found in organisms ranging from prokaryotes to humans. In Drosophila melanogaster, the period (per) gene, which is required for clock function, is transcribed in a circadian manner. We have identified a circadian transcriptional enhancer within a 69-bp DNA fragment upstream of the per gene. This enhancer drives high-amplitude mRNA cycling under light-dark-cycling or constant-dark conditions, and this activity is per protein (PER) dependent. An E-box sequence within this 69-bp fragment is necessary for high-level expression, but not for rhythmic expression, indicating that PER mediates circadian transcription through other sequences in this fragment.
Marqueurs protéiques de la tendreté de la viande bovine: étude prédictive et fonctionnelle

La variabilite non maitrisee de la tendrete de la viande bovine est un probleme majeur pour la filiere industrielle, cette qualite etant tres recherchee par les consommateurs. Depuis de nombreuses annees, differents programmes de recherche ont identifie des marqueurs ADN, ARN et proteines de la tendrete de la viande bovine, dans des contextes differents. Aux Etats-Unis et en Australie, des recherches ont abouti a la conception de tests efficaces de prediction de la qualite de la viande. Ces tests ne fonctionnent cependant pas sur les elevages francais. La filiere francaise est donc demandeuse de tests de prediction simples permettant de mesurer la tendrete sur l’animal vivant et la carcasse. De tels tests sont inexistants a l’heure actuelle. L’objectif de la these est de valider ou non une liste de potentiels marqueurs proteiques de la tendrete, et de developper des equations de prediction de cette qualite, afin d’envisager la conception de tests immunologiques de prediction a destination de la filiere sur l’animal vivant et la carcasse, dans un avenir proche. Une nouvelle technique pour quantifier les proteines d’un echantillon de muscle bovin a ete developpee, le Dot-Blot, et permet de disposer de prototype pour de futurs tests de phenotypage de la tendrete. L’utilisation de cette technique a permis de quantifier 24 proteines sur 111 echantillons de deux muscles de boeufs et de taurillons de race Charolaise. Ces travaux ont identifie en premier lieux des effets biologiques lies au type de muscle et d’animal sur l’abondance des proteines. Puis, trois analyses differentes, dont les resultats sont concordants, ont valide des marqueurs de tendrete et mis en lumiere les principaux mecanismes cellulaires impliques dans la tendrete, generant des equations de prediction de la tendrete. Des outils de bioinformatique ont ete construits a partir de donnees experimentales de la these sur les proteines de la tendrete et de bases de donnees humaines, afin de mieux comprendre les mecanismes biologiques impliques dans la mise en place de la tendrete. En conclusion, ce travail de these a developpe un nouvel outil d’analyse et les premieres equations de prediction de la tendrete de la viande bovine fiables sur un systeme centre sur les mâles, supports indispensables au developpement de tests de prediction de la tendrete sur l’animal vivant et la carcasse. De plus, ce travail a permis de mieux comprendre et caracteriser les mecanismes moleculaires impliques dans la mise en place de la tendrete.
The Core Protein of the Chondroitin Sulfate Proteoglycan Phosphacan Is a High-affinity Ligand of Fibroblast Growth Factor-2 and Potentiates Its Mitogenic Activity*

Using a radioligand binding assay we have demonstrated that phosphacan, a chondroitin sulfate proteoglycan of nervous tissue that also represents the extracellular domain of a receptor-type protein tyrosine phosphatase, shows saturable, reversible, high-affinity binding (K d ∼6 nm) to fibroblast growth factor-2 (FGF-2). Binding was reduced by only ∼35% following chondroitinase treatment of the proteoglycan, indicating that the interaction is mediated primarily through the core protein rather than the glycosaminoglycan chains. Immunocytochemical studies also showed an overlapping localization of FGF-2 and phosphacan in the developing central nervous system. At concentrations of 10 μg protein/ml, both native phosphacan and the core protein obtained by chondroitinase treatment potentiated the mitogenic effect of FGF-2 (5 ng/ml) on NIH/3T3 cells by 75–90%, which is nearly the same potentiation as that produced by heparin at an equivalent concentration. Although studies on the role of proteoglycans in mediating the binding and mitogenic effects of FGF-2 have previously focused on cell surface heparan sulfate, our results indicate that the core protein of a chondroitin sulfate proteoglycan may also regulate the access of FGF-2 to cell surface signaling receptors in nervous tissue.
Validation of a computational knee joint model using an alignment method for the knee laxity test and computed tomography.

BACKGROUND Computational models have been identified as efficient techniques in the clinical decision-making process. However, computational model was validated using published data in most previous studies, and the kinematic validation of such models still remains a challenge. Recently, studies using medical imaging have provided a more accurate visualization of knee joint kinematics. OBJECTIVE The purpose of the present study was to perform kinematic validation for the subject-specific computational knee joint model by comparison with subject's medical imaging under identical laxity condition. METHODS The laxity test was applied to the anterior-posterior drawer under 90° flexion and the varus-valgus under 20° flexion with a series of stress radiographs, a Telos device, and computed tomography. The loading condition in the computational subject-specific knee joint model was identical to the laxity test condition in the medical image. RESULTS Our computational model showed knee laxity kinematic trends that were consistent with the computed tomography images, except for negligible differences because of the indirect application of the subject's in vivo material properties. CONCLUSIONS Medical imaging based on computed tomography with the laxity test allowed us to measure not only the precise translation but also the rotation of the knee joint. This methodology will be beneficial in the validation of laxity tests for subject- or patient-specific computational models.
THE EXTENSIONAL RHEOLOGY OF SEMI-DILUTE DNA SOLUTIONS

The rheological behaviour of polymer melts and dilute polymer solutions in elongational flow have been extensively examined through experiments and theore tical predictions. However, an experimental study to understand the extensional rheological properties o f polymer solutions in the semi-dilute regime is curr ently lacking in terms of a systematic examination of the ffects of concentration and molecular weight. Previous experiments have demonstrated that significant viscoelastic effects characterize the rheological b ehaviour of semi-dilute polymer solutions in shear flows. It is anticipated that these effects will be even more pronounced in extensional flows, which play a criti cal role in a number of industrial contexts such as fibre sp inning and ink-jet printing. In this work, the extensional rheology of linear DNA molecules in a wide range of molecula r weights (25 – 289 kilobasepairs) and concentrations has been investigated. DNA solutions are now routinely used as model polymeric systems due to their near-perfec t monodispersity. A filament stretching rheometer has been used since it is the most reliable method for obtai n ng an estimate of the elongational stress growth of a pol ymer solution. Steady-state uniaxial extensional viscosi ties of DNA dissolved in a viscous solvent under excess sal t conditions have been determined in the semi-dilute regime at room temperature. The dependence of the steady s tate uniaxial extensional viscosities on concentration i s observed to display universal behaviour, which can be validated by theoretical predictions. The material functions obtained in this work will provide benchmark data that are useful for the characterization of these industrially important systems.
Increased expression of iASPP, regulated by hepatitis B virus X protein-mediated NF-κB activation, in hepatocellular carcinoma.

BACKGROUND & AIMS iASPP is an inhibitory member of the ankyrin-repeat-, SH3-domain- and proline-rich-region-containing protein (ASPP) family; iASPP expression is up-regulated in different human tumor types. We explored the molecular mechanism increased expression of iASPP and its role in hepatocellular carcinoma (HCC). METHODS iASPP expression levels in human liver samples and cell lines were determined by polymerase chain reaction, immunoblot, and immunohistochemical analyses. Luciferase reporter, chromatin immunoprecipitation, and electrophoretic mobility shift assays were used to measure transcriptional activation by nuclear factor-κB (NF-κB). Effects on tumor growth were characterized with MTS, soft agar colony formation, and flow cytometry analyses. Tumorigenicity of cells was studied in nude mice. RESULTS Compared with normal liver cells or tissues, iASPP was expressed at significantly higher levels in HCC cell lines (9/14) and liver samples from patients with HCC, cirrhosis, or hepatitis B virus infection. Increased expression of iASPP was significantly associated with time to recurrence and survival time of patients with HCC. NF-κB activation increased the expression of iASPP through p65/p50 binding to a putative NF-κB-binding site in the iASPP promoter; hepatitis B virus X gene product might up-regulate expression of iASPP. Transgenic expression of iASPP promoted tumor cell proliferation and resistance to chemotherapeutic drugs in vitro and in vivo. CONCLUSIONS iASPP is up-regulated in HCC; it is a direct transcription target of NF-κB. Increased iASPP expression contributes to tumor progression by proliferative and antiapoptotic effects. iASPP might be developed as an HCC therapeutic target or to sensitize cancer cells to chemotherapeutic drugs; it might also be used as a prognostic factor.
Universality for Orders and Graphs Which Omit Large Substructures

This paper will examine universality spectra for relational theories which cannot be described in first-order logic. We will give a method using functors to show that two types of structures have the same universality spectrum. A combination of methods will be used to show universality results for certain ordered structures and graphs. In some cases, a universal spectrum under GCH will be obtained. Since the theories are not first-order, the classic model theory result under GCH does not hold.
Complex N-Acetylation of Triethylenetetramine

Triethylenetetramine (TETA) is an efficient copper chelator that has versatile clinical potential. We have recently shown that spermidine/spermine-N1-acetyltransferase (SSAT1), the key polyamine catabolic enzyme, acetylates TETA in vitro. Here, we studied the metabolism of TETA in three different mouse lines: syngenic, SSAT1-overexpressing, and SSAT1-deficient (SSAT1-KO) mice. The mice were sacrificed at 1, 2, or 4 h after TETA injection (300 mg/kg i.p.). We found only N1-acetyltriethylenetetramine (N1AcTETA) and/or TETA in the liver, kidney, and plasma samples. As expected, SSAT1-overexpressing mice acetylated TETA at an accelerated rate compared with syngenic and SSAT1-KO mice. It is noteworthy that SSAT1-KO mice metabolized TETA as syngenic mice did, probably by thialysine acetyltransferase, which had a Km value of 2.5 ± 0.3 mM and a kcat value of 1.3 s−1 for TETA when tested in vitro with the human recombinant enzyme. Thus, the present results suggest that there are at least two N-acetylases potentially metabolizing TETA. However, their physiological significance for TETA acetylation requires further studies. Furthermore, we detected chemical intramolecular N-acetyl migration from the N1 to N3 position of N1AcTETA and N1,N8-diacetyltriethylenetetramine in an acidified high-performance liquid chromatography sample matrix. The complex metabolism of TETA together with the intramolecular N-acetyl migration may explain the huge individual variations in the acetylation rate of TETA reported earlier.
Needlestick and Sharp Injuries among Nursing and Midwifery Students

Background: The students, especially studying at health school students, due to their inadequacy in clinical experience and technical knowledge are at high risk of exposing themselves to blood-borne pathogens vi a needlestick and sharp injury. Aim: The aim of the study was to investigate the freque ncy of needlestick and sharp injuries among nursing and midwifery students, the affecting factors and t he practices related to the subject. Methodology: This study was descriptively and retrospectively p erformed using a questionnaire. It consisted of 18 questions that were composed of the questions co ncerning demographic features of the students and r elated to needlestick and sharp injuries. The study was perfo rmed in 434 students from the Health School of Selc uk University and accepting to participate in the stud y. Results: Descriptive statistics and chi-square were used fo r data analysis. It was determined that among the students, 83.9% were exposed to needlestick and sha rp, 66.2% did not wear gloves while performing the practices, 77.9% were vaccinated against hepatitis B, and 91.7% were injured while breaking ampoules. Conclusions: As a consequence of the study, the rate of the use of protective methods was detected to be decreased although the rate of needlestick and sharp was high among health school students.
Dietary patterns and the risk of obesity, type 2 diabetes mellitus, cardiovascular diseases, asthma, and neurodegenerative diseases

ABSTRACT Diet and lifestyle play a significant role in the development chronic diseases; however the full complexity of this relationship is not yet understood. Dietary pattern investigation, which reflects the complexity of dietary intake, has emerged as an alternative and complementary approach for examining the association between diet and chronic diseases. Literature on this association has largely focused on individual nutrients, with conflicting outcomes, but individuals consume a combination of foods from many groups that form dietary patterns. Our objective was to systematically review the current findings on the effects of dietary patterns on chronic diseases. In this review, we describe and discuss the relationships between dietary patterns, such as the Mediterranean, the Dietary Approach to Stop Hypertension, Prudent, Seventh-day Adventists, and Western, with risk of obesity, type-2 diabetes mellitus, cardiovascular diseases, asthma, and neurodegenearive diseases. Evidence is increasing from both observational and clinical studies that plant-based dietary patterns, which are rich in fruits, vegetables, and whole grains, are valuable in preventing various chronic diseases, whereas a diet high in red and processed meat, refined grains and added sugar seems to increase said risk. Dietary pattern analysis might be especially valuable to the development and evaluation of food-based dietary guidelines.
The effects of fasting on the thermogenic, metabolic and cardiovascular responses to infused adrenaline

The effects of fasting on the thermogenic, lipolytic and cardiovascular responses to adrenaline were examined in nine normal, young, non-obese subjects. Each subject attended for study after 12, 36 and 72 h fasting. After basal measurements adrenaline was infused at 25 ng/min per kg ideal body weight for 90 min. Fasting increased the thermogenic effect of the adrenaline (mean 14·6 (SE 1·7), 16·6 (SE 1·8), 22·6 (SE 1·6) J/:min per kg fat-free mass after 12, 36 and 72 h fasting respectively; P < 0·001, ANOVA). Basal plasma palmitate turnover increased with duration of fasting (1·48 (SE 0·22), 1·95 (SE 0·34) and 2·26 (SE 0·33) μmol/min per kg body weight: P < 0·001, ANOVA), but the response to adrenaline was unaffected by fasting. The percentage values for basal plasma palmitate turnover oxidized were 44 (SE 2; 12 h), 46 (SE 5; 36 h) and 42 (SE 4)% (72 h). In response to adrenaline this percentage fell, suggesting that adrenaline infusion may favour intra-tissue lipid oxidation.
Minimum C5‐saturated graphs

A graph is C5‐saturated if it has no five‐cycle as a subgraph, but does contain a C5 after the addition of any new edge. We prove that the minimum number of edges in a C5 ‐saturated graph on n≥11 vertices is sat(n, C5)=⌈10(n−1)/7⌉−1 if n∈N0={11, 12, 13, 14, 16, 18, 20} and is ⌈10(n−1)/7⌉ if n≥11 and n∉N0. © 2009 Wiley Periodicals, Inc. J Graph Theory
Relationship between udder morphology traits, alveolar and cisternal milk compartments and machine milking performances of dairy camels (Camelus dromedarius)

A total of 22 dairy dromedary camels under intensive conditions in late lactation (275±24 days) were used to study the relationship between external and internal udder morphology and machine milking performances. Measurements of udder and teat morphology were obtained immediately before milking and in duplicate. Individual milk yield, lag time and total milking time were recorded during milking, and milk samples were collected and analyzed for milk composition thereafter. Cisternal and alveolar milk volumes and composition were evaluated at 9 h milking interval. Results revealed that dairy camels had well developed udders and milk veins, with medium sized teats. On average, milk yield as well as milk fat and protein contents were 4.80±0.50 L d-1, 2.61±0.16% and 3.08±0.05%, respectively. The low fat values observed indicated incomplete milk letdown during machine milking. Lag time, and total milking time were 3.0±0.3, and 120.0±8.9s, on average, respectively. Positive correlations (p<0.05) were observed between milk yield and udder depth (r=0.37), distance between teats (r=0.57) and milk vein diameter (r=0.28), while a negative correlation was found with udder height (r=-0.25, p<0.05). Cisternal milk accounted for 11% of the total udder milk. Positive correlations were observed between total milk yield and volume of alveolar milk (r=0.98; p<0.001) as well as with volume of cisternal milk (r=0.63, p<0.05). Despite the low udder milk storage capacity observed in dairy camels, our study concluded that the evaluated dromedary sample had adequate udder morphology for machine milking. Finally, positive relationships were detected between milk yield and udder morphology traits of dairy camels.
N‐Acetyl‐l‐cysteine protects SHSY5Y neuroblastoma cells from oxidative stress and cell cytotoxicity: effects on β‐amyloid secretion and tau phosphorylation

Redox changes within neurones are increasingly being implicated as an important causative agent in brain ageing and neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD) and Alzheimer's disease (AD). Cells have developed a number of defensive mechanisms to maintain intracellular redox homeostasis, including the glutathione (GSH) system and antioxidant enzymes. Here we examine the effects of N‐acetyl‐l‐cysteine (NAC) on β‐amyloid (Aβ) secretion and tau phosphorylation in SHSY5Y neuroblastoma cells after exposure to oxidative stress inducing/cytotoxic compounds (H2O2, UV light and toxic Aβ peptides). Aβ and tau protein are hallmark molecules in the pathology of AD while the stress factors are implicated in the aetiology of AD. The results show that H2O2, UV light, Aβ1–42 and toxic Aβ25–35, but not the inactive Aβ35–25, produce a significant induction of oxidative stress and cell cytotoxicity. The effects are reversed when cells are pre‐treated with 30 mm NAC. Cells exposed to H2O2, UV light and Aβ25–35, but not Aβ35–25, secrete significantly higher amounts of Aβ1–40 and Aβ1–42 into the culture medium. NAC pre‐treatment increased the release of Aβ1–40 compared with controls and potentiated the release of both Aβ1–40 and Aβ1–42 in Aβ25–35‐treated cells. Tau phosphorylation was markedly reduced by H2O2 and UV light but increased by Aβ25–35. NAC strongly lowered phospho‐tau levels in the presence or absence of stress treatment.
Does the Stria Terminalis Carry Information Concerning Feeding and Body Weight Regulation from the Posterodorsal Amygdala to the Hypothalamus

This Dissertation is protected by copyright and/or related rights. It has been brought to you by ScholarWorks@UNO with permission from the rights-holder(s). You are free to use this Dissertation in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s) directly, unless additional rights are indicated by a Creative Commons license in the record and/ or on the work itself.