Miracle-dz/UniFAR-FTUs huggingface dataset:
Associated factors of prenatal depression among teenage pregnant women at King Chulalongkorn Memorial Hospital.

OBJECTIVE Depression during pregnancy is associated with deteriorating maternal health and increasing risk of preterm birth, fetal growth restriction, and suicidal attempt. The problems may be worse in adolescents who are more vulnerable. This study was conducted to determine the percentage of depression among teenage mothers and its associated factors. MATERIAL AND METHOD Two hundred teenage pregnant women aged between 13 and 19 years who visited King Chulalongkorn Memorial Hospital (KCMH) participated in the present study. They were asked to complete the validated Thai Edinburgh Postnatal Depression Scale (EPDS) questionnaire for depression screening. The cut-offscore of 11 was used for the diagnosis of prenatal depression. RESULTS Ninety-two (46%) teenage pregnant women were found to have prenatal depression using the EPDS cut-off score of 11. The mean age of participants was 17.5 years with the mean gestational ages of 23 weeks. Most of the participants (67%) resignedfrom school and 16% had history of attempted abortion during current pregnancy. There was no significant association between prenatal depression and unplanned pregnancy, unemployment, leaving school, or trimester at screening. Logistic regression analyses showed that history of attempted abortion and inadequate income were significantly associated with prenatal depression (odd ratio = 8.03, 95% CI 1.59 to 40.37 and 4.16, 95% CI 1.35 to 12.83, respectively). CONCLUSION Prenatal depression was common among teenage pregnant women who visited KCMH. Attempted abortion and inadequate income were found to be significantly associated with prenatal depression.
TWO NEW SPECIES OF CARDICOLA (DIGENEA: SANGUINICOLIDAE) IN DRUMS (SCIAENIDAE) FROM MISSISSIPPI AND LOUISIANA

Two new species of Cardicola (Digenea: Sanguinicolidae) are described from the heart of drums (Perciformes: Sciaenidae) in and off Mississippi and Louisiana. Cardicola palmeri n. sp. infects the black drum, Pogonias cromis, and, in mature individuals, is distinguished from its congeners by the combination of a body 1.9–2.9 times longer than wide, an esophagus 44–52% of the body length, posterior ceca 1.0–1.8 times longer than the anterior ones, a gland encircling the seminal vesicle that is 30–37% of maximum body width in diameter, and a sinistral and nearly medial male pore. Cardicola currani n. sp. infects the red drum, Sciaenops ocellatus, and is distinguished from its congeners by the combination of posterior ceca 1.8–4.2 times longer than anterior ones, a male pore located well posterior to the ootype, a female pore and transverse metraterm located just anterior to the level of the ootype, and a medial and posttesticular ovary located at the level of the terminal ends of the posterior ceca. No adult blood fluke had been described previously from the northern Gulf of Mexico west of Florida. Each of the 2 flukes infected only 1 host species despite the hosts being sympatric.
Analysis of variation of bovine viral diarrhoea virus E 2 sequence following transplacental infection of cattle

The genetic and antigenic diversity observed in field isolates of bovine viral diarrhoea virus (BVDV) is thought to occur during acute infection because of the genetic stability observed in BVDV throughout the lifetime of persistently infected (PI) cattle. In this study, 15 cows in early pregnancy were inoculated with identical challenge doses obtained from a single infectious inoculum of the virologically cloned isolate Pe515nc. In order to examine the diversity that may develop in utero in the PI foetus, the variable E2 sequence of the virus isolated directly from the serum of each PI calf was compared. A high degree of sequence similarity was demonstrated, with 0–4 nucleotide differences out of 608 bases compared. Thus, the virus showed relatively few genomic changes in any of the PI calves, although we observed that the in utero environment did provide some opportunity for genetic variation to become established. # 2004 Elsevier B.V. All rights reserved.
Mechanism of Mitogen-Activated Protein Kinase Activation by Gonadotropin-Releasing Hormone in the Pituitary αT3-1 Cell Line: Differential Roles of Calcium and Protein Kinase C.

The mechanism of mitogen-activated protein kinase (MAPK, ERK) stimulation by the GnRH analog [d-Trp6]GnRH (GnRH-a) was investigated in the gonadotroph-derived αT3–1 cell line. GnRH-a as well as the protein kinase C (PKC) activator 12-O-tetradecanoyl phorbol-13-acetate (TPA) stimulated a sustained response of MAPK activity, whereas epidermal growth factor (EGF) stimulated a transient response. MAPK kinase (MEK) is also activated by GnRH-a, but in a transient manner. GnRH-a and TPA apparently activated mainly the MAPK isoform ERK1, as revealed by Mono-Q fast protein liquid chromatography followed by Western blotting as well as by gel kinase assay. GnRH-a and TPA stimulated the tyrosine phosphorylation of several proteins, and this effect as well as the stimulation of MAPK activity were inhibited by the PKC inhibitor GF 109203X. Similarly, down-regulation of TPA-sensitive PKC subspecies nearly abolished the effect of GnRH-a and TPA on MAPK activity. Furthermore, the protein tyrosine kinase (PTK) inhibitor ge.
Characterization of Binding of Leukotriene C4 by Human Multidrug Resistance Protein 1

Multidrug resistance protein 1 (MRP1) is capable of actively transporting a wide range of conjugated and unconjugated organic anions. The protein can also transport additional conjugated and unconjugated compounds in a GSH- or S-methyl GSH-stimulated manner. How MRP1 binds and transports such structurally diverse substrates is not known. We have used [3H]leukotriene C4 (LTC4), a high affinity glutathione-conjugated physiological substrate, to photolabel intact MRP1, as well as fragments of the protein expressed in insect cells. These studies revealed that: (i) LTC4 labels sites in the NH2- and COOH-proximal halves of MRP1, (ii) labeling of the NH2-half of MRP1 is localized to a region encompassing membrane-spanning domain (MSD) 2 and nucleotide binding domain (NBD) 1, (iii) labeling of this region is dependent on the presence of all or part of the cytoplasmic loop (CL3) linking MSD1 and MSD2, but not on the presence of MSD1, (iv) labeling of the NH2-proximal site is preferentially inhibited byS-methyl GSH, (v) labeling of the COOH-proximal half of the protein occurs in a region encompassing transmembrane helices 14–17 and appears not to require NBD2 or the cytoplasmic COOH-terminal region of the protein, (vi) labeling of intact MRP1 by LTC4 is strongly attenuated in the presence of ATP and vanadate, and this decrease in labeling is attributable to a marked reduction in LTC4 binding to the NH2-proximal site, and (vii) the attenuation of LTC4 binding to the NH2-proximal site is a consequence of ATP hydrolysis and trapping of Vi-ADP exclusively at NBD2. These data suggest that MRP1-mediated transport involves a conformational change, driven by ATP hydrolysis at NBD2, that alters the affinity with which LTC4 binds to one of two sites composed, at least in part, of elements in the NH2-proximal half of the protein.
Delivering Evidence-Based Upper Limb Rehabilitation for Children with Cerebral Palsy: Barriers and Enablers Identified by Three Pediatric Teams

ABSTRACT This study aimed to identify barriers and enablers experienced by occupational therapists to delivering evidence-based upper limb intervention for children with unilateral cerebral palsy. Semistructured interviews informed by the Theoretical Domains Framework were conducted with nine occupational therapists from three teams to ascertain barriers and enablers to implementing five evidence criteria. A key barrier was lack of knowledge of current evidence for upper limb therapies for children with unilateral cerebral palsy. Therapists were confident in delivering goal-directed bimanual occupational therapy, but less knowledgeable and skilled, and hence confident in providing constraint therapy. Strategies to increase dose of therapy were identified as greater use of home programs and group-based interventions; however, therapists indicated the need for further education and skill development in these areas. In order to increase the uptake of research evidence into practice, findings from this study will be used to inform context-specific, individually targeted implementation strategies.
Amygdaloid CRF Pathways: Role in Autonomic, Neuroendocrine, and Behavioral Responses to Stress

The results of numerous studies have provided compelling evidence that CRF plays an important function in the amygdala. Stimulation of the amygdala produces physiological changes similar those observed after central injections of CRF. Central injections of CRF activate neurons in the amygdala as measured by increases in c-fos protein expression. Destruction of cells or injections of CRF antagonist in the amygdala can attenuate some of the central effects of CRF. The amygdala is the origin of major CRF-containing pathways in the brain. Amygdaloid CRF neurons project to widespread regions of the basal forebrain and brain stem. These amygdaloid pathways mainly arise from the central amygdaloid nucleus where there are a large number of CRF immunoreactive neuronal perikarya. Glucocorticoid and CRF-binding protein are located in cells of the central amygdaloid nucleus. CRF neurons in the central nucleus send their axons to the bed nucleus of the stria terminalis, lateral hypothalamus, midbrain central gray, raphe nuclei, parabrachial region, and the nucleus of the solitary tract. Tract tracing studies have suggested that amygdaloid CRF neurons also innervate CRF neurons in some of these regions and, furthermore, that CRF neurons in some of these areas project back to the CRF neurons in the amygdala. Thus, the amygdala is part of a network of brain nuclei interconnected by CRF pathways. In addition, amygdaloid CRF neurons may project directly to dopaminergic, noradrenergic, and serotonergic neurons, which have widespread projections throughout the neuroaxis. (ABSTRACT TRUNCATED AT 250 WORDS)
Population Screening and Prevention Strategies for Thalassemias and other Hemoglobinopathies of Eastern India: Experience of 18,166 cases

Abstract We evaluated population screening programs (1999–2011), conducted by the Thalassaemia Foundation, Kolkata, India, for the first time in Eastern India in different districts of West Bengal, for prevention of thalassemia comprising screening of heterozygotes and β-thalassemia intermedia (β-TI) cases [β+, β++, β0/β+, βE/βE (codon 26 or HBB: c.79G > A), Hb-E-β-thalassemia (Hb E-β-thal)]. Among 18,166 cases, we found 2092 heterozygotes and 2245 β-TI individuals (who had no information about their disorders). Results were evaluated with standard hematological analyses including erythrocyte indices, hemoglobin (Hb) typing and quantification. Participants were divided into five groups (children, pre-marriage cases, pre-pregnancy cases, affected family members, pregnant women). The objectives of this evaluation were to fix cut-off values of red blood cells (RBCs), mean corpuscular volume (MCV), mean corpuscular Hb (MCH), red blood cell distribution width (RDW) and Hb A2, as the standard World Health Organization (WHO) guidelines were not strictly followed in mass-scale screening programs. We have observed many dilemmas in considering the status of the thalassemia subject, due to presence of some other clinical conditions such as iron deficiency anemia, α-thalassemia (α-thal), δ-thalassemia (δ-thal), clinically silent Hb variants, and some cases of non hemoglobinopathies (such as pregnancy) along with thalassemia. The MCV values varied greatly in different conditions of hemoglobinopathies, whereas MCH provided a more stable measurement. We found an MCH value of <27.0 pg is a suitable cut-off point for screening in this population. Participants with an MCH of <27.0 pg should be investigated further to confirm or exclude a diagnosis of β-thal trait.
Impaired Innate Immune Function Associated with Fecal Supernatant from Crohn's Disease Patients: Insights into Potential Pathogenic Role of the Microbiome

Background:Although a dysbiosis of the intestinal microbiome plays a role in the pathogenesis of Crohn's disease (CD), the functional implication is unclear. We sought to determine the influence of fecal supernatant from patients with CD on innate immune function in neutrophil, macrophage, and epithelial cells. Metabolomic analysis was subsequently performed in an attempt to identify potential compounds responsible for the effects identified. Methods:In the fecal samples from 11 pediatric patients with CD and 10 healthy controls, 16S ribosomal and metabolomic analyses were performed. We evaluated the effect of preincubation with fecal supernatant on neutrophil, macrophage, epithelial cell survival, superoxide production, bacterial invasion, and/or bactericidal function using gentamicin protection assay. Ten substances identified as most elevated in CD compared with control samples by metabolomic analysis were similarly tested for effect on bactericidal function. Results:There were no statistically significant differences in microbial membership in fecal samples from patients with CD compared with healthy controls. However, bactericidal function was impaired in neutrophils and monocytes preincubated with supernatant from fecal samples from patients with CD. Although levels of many metabolites were noted to be altered in samples from patients with CD, the combination of the 10 most elevated compounds failed to demonstrate any effect on neutrophil bactericidal capacity. Conclusions:Fecal supernatant from patients with CD impairs intracellular bactericidal activity in neutrophils and macrophages. The functional consequences of the intestinal microbiome and its associated secreted products on innate immune function may be more critical than microbial membership in understanding the pathophysiology of CD.
Is Posttraumatic Epilepsy the Best Model of Posttraumatic Epilepsy?

A Model of Posttraumatic Epilepsy Induced by Lateral Fluid-Percussion Brain Injury in Rats Kharatishvili I, Nissinen JP, McIntosh TK, Pitkänen A Neuroscience 2006;140:685–697 Although traumatic brain injury is a major cause of symptomatic epilepsy, the mechanism by which it leads to recurrent seizures is unknown. An animal model of posttraumatic epilepsy that reliably reproduces the clinical sequelae of human traumatic brain injury is essential to identify the molecular and cellular substrates of posttraumatic epileptogenesis, and perform preclinical screening of new antiepileptogenic compounds. We studied the electrophysiologic, behavioral, and structural features of posttraumatic epilepsy induced by severe, non-penetrating lateral fluid-percussion brain injury in rats. Data from two independent experiments indicated that 43% to 50% of injured animals developed epilepsy, with a latency period between 7 weeks to 1 year. Mean seizure frequency was 0.3 ± 0.2 seizures per day and mean seizure duration was 113 ± 46 s. Behavioral seizure severity increased over time in the majority of animals. Secondarily generalized seizures comprised an average of 66 ± 37% of all seizures. Mossy fiber sprouting was increased in the ipsilateral hippocampus of animals with posttraumatic epilepsy compared with those subjected to traumatic brain injury without epilepsy. Stereologic cell counts indicated a loss of dentate hilar neurons ipsilaterally following traumatic brain injury. Our data suggest that posttraumatic epilepsy occurs with a frequency of 40% to 50% after severe non-penetrating fluid-percussion brain injury in rats, and that the lateral fluid percussion model can serve as a clinically relevant tool for pathophysiologic and preclinical studies.
The Influence of IT Management Practice on IT Use in Large Organizations

This paper draws upon the absorptive capacity as the theoretical basis for a pragmatic explanation of key factors affecting information technology (IT) use in large, complex organizations. IT use is defined as the extent to which an organization deploys IT to support operational and strategic tasks. The study uses results from a survey of senior IT managers from 132 firms to examine hypothesized relationships among the following constructs: IT management climate, managerial IT knowledge, IT-management-process effectiveness, and IT use. A structural-equation model is developed using LISREL to assess the relative effects of and interrelationships among these constructs. The study's findings indicate that managerial IT knowledge is a dominant factor in explaining high levels of IT use and that both managerial IT knowledge and IT-management-process effectiveness are influenced by IT management climate.
Sporadic Burkitt lymphoma in southern China: 12 years' experience in a single institution in Guangzhou

Aim To analyse the clinicopathological features of sporadic Burkitt lymphoma (sBL). Methods In a review of 1682 cases of non-Hodgkin lymphoma diagnosed in the First Affiliated Hospital and Zhongshan School of Medicine, from 1998 to 2010, 20 cases (1.2%) of sBL were identified. Histopathological examination, immunohistochemistry and in situ hybridisation were used to analyse the clinicopathological features of these cases. Results Of the 20 cases of sBL, 18 patients were male and two were female. The mean age was 18 years (range 2–67 years). Extranodal presentation was more common than nodal presentation (55% vs 15%). Histopathologically, 18 cases (90%) showed monotonous medium-sized tumour cells, and two cases showed cells that were slightly pleomorphic in nuclear size and shape. Immunophenotypically, MUM1 was positive in three of 17 cases (17.6%). EBER expression was shown in five of 17 cases (29.4%), and all EBER-positive sBLs were Bcl-6+/MUM1−. Conclusion sBL is rare and mainly affects male children, with predominantly extranodal presentation. MUM1 expression was found in some sBLs. EBER expression was found in 29.4% of sBLs from southern China, an area with a well-known high incidence of nasopharyngeal carcinoma, which is closely associated with Epstein–Barr virus infection.
The Problem of Female Infanticide in the Greco-Roman World

A FTER careful analysis of the literary evidence, earlier studies concerning the exposure of children (and any resultant infanticide) have established that the practice was of negligible importance in Greek and Roman society. ' Recent attempts to draw conclusions from skeletal remains and other archaeological evidence that suggest male-female sex ratio imbalances and possible high rates of female infanticide are not convincing.2 This analysis will attempt to show that, in fact, a high rate of female infanticide in antiquity was demographically impossible, and a rate of more than a few percent of live female births per year was highly improbable for more than a short period. It has long been recognized that osteological evidence concerning the sex ratio and age structure of ancient populations is systematically biased against females and infants.3 The survival of skeletal material depends to a large extent on the size and thickness of the bones and since infants and women have smaller, thinner bones than men, fewer are likely to survive. Furthermore, excavators are more likely to discard smaller, broken specimens, thus distorting the age of the remains upward and biasing the sex ratio towards males. Since health factors producing tougher skeletons also favor physical well-being and therefore greater longevity, the loss of weaker skeletons would also distort the age structure upward. Few infants, even
The Effect of Brand on the Impact of e-WOM on Hotels’ Financial Performance

ABSTRACT Travelers increasingly consider electronic word of mouth (e-WOM) from online review platforms when making their accommodation decisions. Theory predicts that e-WOM increases sales, but little is known regarding the role that branding plays in the relation between e-WOM and hotels’ financial performance. This study relied on financial data (including Revenue per Available Room—RevPAR—and sales profitability) and 34,164 online customer reviews gathered from TripAdvisor (as a proxy for e-WOM) based on panel data from 221 hotels, including not-branded and branded chain hotels in France from 2005 to 2013. The results show that the volume of reviews has no effect on RevPAR growth for branded chain hotels and a positive effect on RevPAR growth for not-branded chain hotels. In addition, the direct effect of the valence of online reviews and its interaction effect with the yearly and cumulative volume of online reviews on RevPAR growth and sales profitability is shown to apply to not-branded chain hotels but not to branded chain hotels. This study contributes to the e-WOM literature by revealing the role of a brand in e-WOM’s impact on hotel financial performance. Based on our results, managers of branded chain hotels should know that they might find it difficult to leverage e-WOM to achieve higher RevPAR growth and greater sales profitability, as their economic advantages derive from their brand instead of e-WOM.
Hepatic resection for hepatocellular carcinoma. Clinical features and long-term prognosis.

Ninety-eight hepatic resections for hepatocellular carcinoma were performed on 94 patients from 1963–1985. HBs antigen was positive in 17% of patients, preoperative serum alpha-fetoprotein was more than 20 ng/mL in 70% of patients, and liver cirrhosis was present in 75% of patients. Hospital mortali
Protein content and functional characteristics of serum‐purified exosomes from patients with colorectal cancer revealed by quantitative proteomics

Tumor cells of colorectal cancer (CRC) release exosomes into the circulation. These exosomes can mediate communication between cells and affect various tumor‐related processes in their target cells. We present a quantitative proteomics analysis of the exosomes purified from serum of patients with CRC and normal volunteers; data are available via ProteomeXchange with identifier PXD003875. We identified 918 proteins with an overlap of 725 Gene IDs in the Exocarta proteins list. Compared with the serum‐purified exosomes (SPEs) of normal volunteers, we found 36 proteins upregulated and 22 proteins downregulated in the SPEs of CRC patients. Bioinformatics analysis revealed that upregulated proteins are involved in processes that modulate the pretumorigenic microenvironment for metastasis. In contrast, differentially expressed proteins (DEPs) that play critical roles in tumor growth and cell survival were principally downregulated. Our study demonstrates that SPEs of CRC patients play a pivotal role in promoting the tumor invasiveness, but have minimal influence on putative alterations in tumor survival or proliferation. According to bioinformatics analysis, we speculate that the protein contents of exosomes might be associated with whether they are involved in premetastatic niche establishment or growth and survival of metastatic tumor cells. This information will be helpful in elucidating the pathophysiological functions of tumor‐derived exosomes, and aid in the development of CRC diagnostics and therapeutics.