Miracle-dz/UniFAR-FTUs huggingface dataset:
Recombinant CLIC1 (NCC27) Assembles in Lipid Bilayers via a pH-dependent Two-state Process to Form Chloride Ion Channels with Identical Characteristics to Those Observed in Chinese Hamster Ovary Cells Expressing CLIC1*

CLIC1 (NCC27) is an unusual, largely intracellular, ion channel that exists in both soluble and membrane-associated forms. The soluble recombinant protein can be expressed in Escherichia coli, a property that has made possible both detailed electrophysiological studies in lipid bilayers and an examination of the mechanism of membrane integration. SolubleE. coli-derived CLIC1 moves from solution into artificial bilayers and forms chloride-selective ion channels with essentially identical conductance, pharmacology, and opening and closing kinetics to those observed in CLIC1-transfected Chinese hamster ovary cells. The process of membrane integration of CLIC1 is pH-dependent. Following addition of protein to the trans solution, small conductance channels with slow kinetics (SCSK) appear in the bilayer. These SCSK modules then appear to undergo a transition to form a high conductance channel with fast kinetics. This has four times the conductance of the SCSK and fast kinetics that characterize the native channel. This suggests that the CLIC1 ion channel is likely to consist of a tetrameric assembly of subunits and indicates that despite its size and unusual properties, it is able to form a completely functional ion channel in the absence of any other ancillary proteins.
The incidence of invasive fungal infections in neutropenic patients with acute leukemia and myelodysplastic syndromes receiving primary antifungal prophylaxis with voriconazole

The objective of this study is to characterize the outcomes of primary antifungal prophylaxis with voriconazole in patients receiving intensive chemotherapy for acute myelogenous leukemia (AML) or myelodysplastic syndromes (MDS). We conducted a single center, retrospective, cohort study of consecutive adult patients with AML or MDS at Mayo Clinic between January 1, 2006 and July 1, 2010. The study included patients undergoing induction or first relapse combination chemotherapy who received voriconazole 200 mg orally twice daily as prophylaxis during the neutropenic phase. Patient records were evaluated until 30 days after neutrophil recovery for development of invasive fungal infection (IFI) as defined per EORTC/MSG 2008 criteria with computed tomography scans independently reviewed by a radiologist. Therapeutic drug monitoring and reasons for voriconazole discontinuation were documented. Twenty four episodes of IFI were detected among 165 consecutive patients for an overall incidence of 145 per 1000 patients. The incidence of IFI was 24, 42, and 78 per 1000 patients for proven, probable, and possible infection, respectively. Four patients developed proven IFI (n = 2 Aspergillus spp., n = 2 Rhizopus spp.). Serum voriconazole trough concentrations were available in 39 patients, and no statistically significant difference in voriconazole trough level was observed between those with versus without an IFI. Voriconazole prophylaxis was discontinued in 81 patients due to suspected IFI (n = 24), fever of unknown origin (n = 19), adverse events (n = 23), and other causes (n = 17). Voriconazole as primary IFI prophylaxis is safe and may be beneficial in AML/MDS patients receiving intensive chemotherapy. Am. J. Hematol. 88:283–288, 2013. © 2013 Wiley Periodicals, Inc.
cDNA expression profiling of chondrosarcomas: Ollier disease resembles solitary tumours and alteration in genes coding for components of energy metabolism occurs with increasing grade

Conventional central chondrosarcomas are malignant cartilaginous tumours, occasionally arising secondary to either solitary or multiple (Ollier disease) enchondromas. Recurrences may have progressed in grade. The aims of the present study were to identify putative differences in gene expression between solitary and Ollier disease‐related tumours, and to elucidate signalling pathways involved in tumour progression by genome‐wide cDNA expression analysis. Arrays enriched for cartilage‐specific cDNAs and genes involved in general tumourigenesis were used to analyse enchondromas (n = 3, two with Ollier disease), chondrosarcomas of different grades (n = 19, three with Ollier disease), normal resting‐zone cartilage (n = 2), and chondrosarcoma cells in culture (n = 7). The arrays were analysed by unsupervised hierarchical clustering, significant analysis of microarray, and T‐tests. Confirmation of data was performed by immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction (RT‐PCR). Ollier disease cases and solitary tumours revealed similar expression profiles, suggesting that the same signalling pathways are involved in tumourigenesis. Interestingly, JunB protein expression was significantly higher in grade I chondrosarcomas than in enchondromas (p = 0.009), which could be of diagnostic relevance. Upon chondrosarcoma progression, matrix‐associated genes are down‐regulated, reflecting the histology of high‐grade tumours. An increase in glycolysis‐associated, and a decrease in oxidative phosphorylation‐related, genes was found in high‐grade tumours. These findings suggest an adaptation in energy supply upon progression towards higher grade. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
A Ilha de Santiago (Cabo Verde)

A ilha de Santiago e a mais extensa do arquipelago de Cabo Verde, foi tambem a ilha onde comecou o povoamento do arquipelago iniciado no seculo XV, durante o periodo de expansao europeia protagonizada pela coroa portuguesa. Partindo de uma paisagem natural sui generis na encruzilhada entre a Macaronesia e o Sahel, a presenca humana iniciada com o povoamento introduziu profundas alteracoes na flora, cobertura vegetal e fauna. O quadro de ciclos de seca e isolamento num periodo de perda de importância na rota de navegacao atlântica, criaram condicoes favoraveis ao surgimento da nacao cabo-verdiana, caracterizada por uma nova lingua, habitos, costumes e modus vivendi que ao longo do tempo se distanciou tanto da antiga metropole colonizadora como das varias culturas ribeirinhas que deram contributo ao seu povoamento. Ao longo de cinco seculos de presenca humana uma ilha antes deserta e num equilibrio fragil foi completamente alterada com a criacao de uma paisagem fortemente culturalizada onde o homem busca o equilibrio entre os fracos recursos naturais e uma economia viavel capaz de promover um desenvolvimento sustentavel e duravel. Apesar da sua localizacao tao proxima da Africa de oeste e sua insercao politica na CEDEAO o arquipelago conseguiu manter a sua originalidade, uma insularidade cultural numa vasta regiao saheliana, mas com grande coesao interna e extensao na sua diaspora pelos varios continentes.
C-Reactive Protein Predicts 96-Week Carotid Intima Media Thickness Progression in HIV-Infected Adults Naive to Antiretroviral Therapy

Abstract:This is a 96-week prospective cohort study of antiretroviral therapy (ART)–naive HIV-infected adults and matched healthy controls to assess progression of carotid intima media thickness (CIMT) and its relationship to inflammation. Median common carotid artery (CCA) CIMT increased significantly but similarly in both groups [CCA: 0.02 (interquartile range: 0–0.05); P < 0.01 within HIV-infected adults vs. 0.01 (0–0.05) mm; P < 0.01 within controls; and P = 0.83 between groups]. Change in bulb CIMT yielded similar results. Independent predictors of CCA CIMT progression in HIV-infected adults were higher systolic blood pressure, total cholesterol, and high sensitivity C-reactive protein. Independent predictors of bulb CIMT progression were higher non–high-density lipoprotein cholesterol and high sensitivity C-reactive protein. Other inflammation markers were not associated with CIMT progression.
Violencia de género y autoestima: efectividad de una intervención grupal

El objetivo de esta investigacion es evaluar la efectividad de una intervencion socioeducativa grupal en una muestrade treinta mujeres que han padecido violencia de genero. El programa de intervencion consta de diez sesiones de noventaminutos de duracion y una frecuencia semanal. La estructura del taller incluye los siguientes elementos: autoevaluaciony autoconcepto, distorsiones cognitivas, critica patologica, pensamiento positivo, establecimiento de metas e identificacionde la manipulacion. Ademas de analizar la efectividad de la intervencion, se relacionan otras variables vinculadascon el proceso: ingresos economicos, estado civil, formacion, historia previa de maltrato o toma de medicacion en lasparticipantes. El instrumento de evaluacion que se ha utilizado ha sido la Escala de Autoestima de Rosenberg (versionespanola de Fernandez-Montalvo & Echeburua, 1997). Se ha aplicado un diseno cuasi-experimental de medidas repetidas(prepost) sin grupo control. Los resultados indicaron que todos los grupos ven incrementado su nivel de autoestimaprevio al inicio del programa. Se discuten las implicaciones de este estudio para investigaciones futuras.
Modeling Magnetic Emissions Combining Image Processing and an Optimization Algorithm

An automated procedure for modeling the radiated emissions of electronic devices is presented in this paper. The principle of the model is to replace the device by a set of electric dipoles defined by their positions, orientations, lengths, and currents. Thus, this modeling procedure aims to determine the number of dipoles and their parameters with a minimum user intervention. The procedure is divided into three parts: the near-field measurements of the tangential components of the magnetic field, an image processing to obtain the number of sources of the model, and the application of an optimization algorithm in order to obtain the different parameters of the model, such as the current and the position of each source.
Submillimetre observations of WISE-selected high-redshift, luminous, dusty galaxies

We present SCUBA-2 (Submillimetre Common-User Bolometer Array) 850 μm submillimetre (submm) observations of the fields of 10 dusty, luminous galaxies at z ∼ 1.7–4.6, detected at 12 and/or 22 μm by the Wide-field Infrared Survey Explorer (WISE) all-sky survey, but faint or undetected at 3.4 and 4.6 μm; dubbed hot, dust-obscured galaxies (Hot DOGs). The six detected targets all have total infrared luminosities greater than 10^(13) L_⊙, with one greater than 10^(14) L_⊙. Their spectral energy distributions (SEDs) are very blue from mid-infrared to submm wavelengths and not well fitted by standard active galactic nuclei (AGN) SED templates, without adding extra dust extinction to fit the WISE 3.4 and 4.6 μm data. The SCUBA-2 850 μm observations confirm that the Hot DOGs have less cold and/or more warm dust emission than standard AGN templates, and limit an underlying extended spiral or ULIRG-type galaxy to contribute less than about 2 or 55 per cent of the typical total Hot DOG IR luminosity, respectively. The two most distant and luminous targets have similar observed submm to mid-infrared ratios to the rest, and thus appear to have even hotter SEDs. The number of serendipitous submm galaxies detected in the 1.5-arcmin-radius SCUBA-2 850 μm maps indicates there is a significant overdensity of serendipitous sources around Hot DOGs. These submm observations confirm that the WISE-selected ultraluminous galaxies have very blue mid-infrared to submm SEDs, suggesting that they contain very powerful AGN, and are apparently located in unusual arcmin-scale overdensities of very luminous dusty galaxies.
Enabling Parents Who Smoke to Prevent Their Children From Initiating Smoking: Results From a 3-Year Intervention Evaluation

REVIEWER COMMENTS. Cigarette smoke is a common respiratory irritant that contributes to exacerbations of childhood asthma and rhinitis. This study provided evidence supporting the association between lower secondhand cigarette-smoke exposure and never smoking among young teenagers, which suggests an additional reason to counsel patients and their families regarding the immediate and long-term risks of exposure to secondhand cigarette smoke and cigarette smoking.
Early Palliative Care and Its Role in Oncology: A Qualitative Study.

INTRODUCTION Early integration of palliative care alongside oncology is being increasingly recommended, although the strategies and models for integration remain poorly defined. We solicited the opinions of patients and caregivers who participated in a randomized trial of early palliative care versus standard oncology care, regarding the respective roles of their oncologist (both groups) and palliative care physician (early palliative care group). MATERIALS AND METHODS The study was performed at a comprehensive cancer center. Forty-eight patients (26 intervention, 22 control) and 23 caregivers (14 intervention, 9 control) were recruited purposefully at trial end. One-on-one, semistructured qualitative interviews were conducted and analyzed using grounded theory. RESULTS The themes resulting from the analysis fell into three categories: the focus of care, the model of care delivery, and the complementarity between teams. The focus of care in oncology was perceived to be disease-centered, with emphasis on controlling disease, directing cancer treatment, and increasing survival; palliative care was perceived to be more holistic and person-focused, with an emphasis on symptom management. Oncology visits were seen as following a structured, physician-led, time-constrained model in contrast to the more fluid, patient-led, flexible model experienced in the palliative care clinic. No differences were found in the descriptions of oncology between participants in the intervention and control groups. Participants in the intervention group explicitly described the roles of their oncologist and their palliative care physician as distinct and complementary. CONCLUSION Participants perceived the respective roles of their oncologist and palliative care physician as discrete, important, and complementary for the provision of excellent cancer care. IMPLICATIONS FOR PRACTICE Patients and their caregivers who experienced early palliative care described the roles of their oncologists and palliative care physicians as being discrete and complementary, with both specialties contributing to excellent patient care. The findings of the present research support an integrated approach to care for patients with advanced cancer, which involves early collaborative care in the ambulatory setting by experts in both oncology and palliative medicine. This can be achieved by more widespread establishment of ambulatory palliative care clinics, encouragement of timely outpatient referral to palliative care, and education of oncologists in palliative care.
Structural Equation Model Tests of Patterns of Family Interaction, Peer Alcohol Use, and Intrapersonal Predictors of Adolescent Alcohol Use and Misuse

A survey of 1,340 students in grades six through twelve was conducted to test, in the context of structural equation models, the predictive validity of a theoretical model of antecedents of adolescent alcohol use and misuse. Constructs including parents' alcohol use, older siblings alcohol use, parents' approval of students alcohol use, older sibling approval of students alcohol use, peer use and approval of alcohol use (PUA), parental nurturance, parental permissiveness, child's grade in school, susceptibility to peer pressure (SPP), and deviant self-image, were included. The final iterations of the models accounted for more than half of the variance in both alcohol use and alcohol misuse. In a standardized solution, the two largest direct effects on both adolescent alcohol use and misuse were from SPP and PUA. When a seven item measure of SPP, including three items specific to alcohol use was used, the SPP latent variable accounted for a somewhat greater percentage of the variance in adolescent alcohol use and misuse than did the latent variable of PUA. When the three alcohol-specific items were deleted from SPP, however, PUA accounted for more variance than SPP. Other predictors, including parental behaviors, proved to be significant when their indirect effects were evaluated. Models predicting alcohol use and alcohol misuse were similar. SPP, PUA, and other significant predictors should be included in future models predicting adolescent alcohol use and misuse, as well as in future interventions targeting these behaviors. Longitudinal studies should be used to test these findings. Evaluation of prevention should include examination of possible interactions of these predictors with each other and with subject subgroup classifications.
Is space expanding in the Friedmann universe models

The interpretation of the expanding universe as an expansion of space has recently been challenged. From the geodesic equation in Friedmann universe models and the empty Milne model, we argue that a Newtonian or special relativistic analysis is not applicable on large scales, and the general relativistic interpretation in terms of expanding space has the advantage of being globally consistent. We also show that the cosmic redshift, interpreted as an expansion effect, contains both the Doppler effect and the gravitational frequency shift.
RFID-enabled traceability in the food supply chain

Purpose – This paper aims to study the main requirements of traceability and examine how the technology of radio frequency identification (RFID) technology can address these requirements. It further seeks to outline both an information data model and a system architecture that will make traceability feasible and easily deployable across a supply chain.Design/methodology/approach – The design research approach is followed, associating traceability requirements to a proposed system design.Findings – The technological approach used has great implications in relation to the cost associated with a traceability system and the ease of its deployment.Research limitations/implications – Validation of the proposed information data model and system architecture is required through practical deployment in different settings.Practical implications – The paper provides practitioners with insight on how RFID technology can meet traceability requirements and what technological approach is more appropriate.Originality/val.
Face discriminative methods across age progression using local k-means ensemble

Face recognition is widely used in many computer vision applications such as surveillance, traffic monitoring, robot vision, access control and so on. However, some problems in face recognition still exist which the expression changes, head movements, accessory occlusion, light changes and aging effect are the main issues. For the aging effect, the shape and texture changes degrade the performance of face recognition. To solve the issue in across age face recognition, we propose face discriminative methods across age progression using local K-means ensemble. First, we find that the gradient angle provides an effective representation. This representation can be improved using hierarchical structure, the K-means pyramid (KMP). KMP demonstrates excellent performance when combined with supervised learning. Experimental results show that the proposed across age methods outperform the existing techniques.
Disease-specific quality of life in patients with lupus nephritis

Background Patient-reported outcomes in lupus nephritis (LN) are not well studied. Studies with disease-targeted PRO tool in LN do not exist. Herein, we describe quality of life (QOL: HRQOL & non-HRQOL) among LN patients using LupusPRO. Methods International, cross-sectional data from 1259 patients with systemic lupus erythematosus (SLE) and LupusPRO were compared, stratified by (a) presence of LN (ACR classification criteria (ACR-LN)) at any time and, (b) active LN (on SLEDAI) at study visit. Damage was assessed by SLICC/ACR-SDI. Multivariate regression analyses for QOL against ACR-LN (active LN) after adjusting for age, gender, ethnicity and country of recruitment were performed. Results Mean (SD) age was 41.7 (13.5) yrs, 93% were women. Five hundred and thirty-nine of 1259 SLE patients had ACR-LN. ACR-LN group was younger, were more often on immunosuppressive medications, had worse QOL on lupus medications and procreation than non-ACR-LN patients. HRQOL and non-HRQOL scores were similar in both groups. One hundred and twenty-nine of 539 ACR-LN patients had active LN. Active LN group was younger, had greater disease activity and had worse HRQOL and non-HRQOL compared to patients without active LN. Specific domains adversely affected were lupus symptoms, lupus medications, procreation, emotional health, body image and desires-goals domains. Patients with ACR-LN and active LN fared significantly worse in lupus medications and procreation HRQOL domains, even after adjusting for age, ethnicity, gender and country of recruitment. Conclusions Lupus nephritis patients have poor QOL. Patients with active LN have worse HRQOL and non-HRQOL. Most domains affected are not included in the generic QOL tools used in SLE. LN patients must receive discussion on lupus medications and procreation issues. Patients with active LN need comprehensive assessments and addressal of QOL, along with treatment for active LN.
Puritanism And Historical Controversy

Part 1 Puritans: William Prynne 1600-69 Lodowicke Muggleton 1609-98 Richard Baxter 1615-91. Part 2 Controversies: puritanism and revolution puritanism and liberty puritanism and capitalism puritanism and witches puritanism and millenarianism puritanism and science.
Evaluation of the Reporting Validity of Central Line–Associated Bloodstream Infection Data to a Provincial Surveillance Program

1. Su S-Y, Chao C-M. Acinetobacter calcoaceticus-Acinetobacter baumannii complex is not equal to A. baumannii. Infect Control Hosp Epidemiol 2013;34:215-216 (in this issue). 2. Kang SJ, Kang C-I, Park SY, et al. Epidemiology and clinical features of community-onset Acinetobacter baumannii infections. Infect Control Hosp Epidemiol 2012;33:1053-1055. 3. Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 2008;21:538582. 4. Gerner-Smidt P, Tjernberg I, Ursing J. Reliability of phenotypic tests for identification of Acinetobacter species. / Clin Microbiol 1991;29:277-282.
Does the Potential to Merge Reduce Competition?

We study anticompetitive horizontal mergers in a dynamic model with noisy collusion. At each instant, firms either privately choose output levels or merge to form a monopoly, trading off the benefits of avoiding price wars against the costs of merging. The potential to merge decreases pre-merger collusion, as punishments effected by price wars are weakened. We thus extend the result of Davidson and Deneckere [Davidson C, Deneckere R (1984) Horizontal mergers and collusive behavior. Internat. J. Indust. Organ. 2(2):117–132. ], who analyzed the weakening of punishments post-merger, demonstrating that pre-merger collusion is weakened, in a fully stochastic model. Thus, although anticompetitive mergers harm competition ex post, the implication is that barriers and costs of merging due to regulation should be reduced to promote competition exante. This paper was accepted by Tomasz Piskorski, finance.