Miracle-dz/UniFAR-FTUs huggingface dataset:
Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes.

Transcription of human papillomavirus type 18 (HPV18) DNA in the human cervical carcinoma cell lines HeLa, C4‐1 and SW756 was studied by nucleotide sequence analysis of HPV18‐positive cDNA clones isolated from a HeLa, C4‐1 and SW756 cDNA library, respectively, and the cDNA sequences were used to predict the potential encoded proteins. The cDNA clones from all three cell lines were found to be derived from virus‐cell fusion transcripts in which 3′‐terminal host cell sequences (different for each cell line) were spliced to 5′‐terminal exon sequences from the HPV18 E6‐E7‐E1 region. Three different types of cDNA clones can be distinguished according to the splicing patterns observed in the 5′ terminal HPV18 sequences. They carry as potential protein‐coding regions the HPV18 specific open reading frames E6 and E6* (generated by splicing and identical with E6 up to the E6* splice junction), E7 and E1 (only in HeLa). Translation of specific cellular genes from the chimeric viral‐cellular transcripts seems to be unlikely. The mapping of the 5′‐ends of the virus‐cell fusion transcripts indicates that transcription is initiated at a viral promoter. The similar patterns of HPV18 transcription in the three different cervical carcinoma cell lines suggest a functional role of HPV18 early genes for the malignant phenotype of these cells.
Apparel Shopping Behavior of Elderly Men and Women

70 interested elderly (65 yr. and over) men and women participated in an investigation of older consumers' apparel-shopping behavior and satisfactions and dissatisfactions with available clothing and facilities. Analysis showed that the participants considered shopping ease, wide variety, and label/brand as some of the important factors while shopping for clothes. The respondents were satisfied with good selection, closeness of the mall, and reasonable sales. However, their dissatisfactions ranged from service to fit and size, youthful styles, and abundance of imported clothing. In-store displays, mail order catalogs, and window shopping were the most commonly used sources of apparel information. Several implications of the findings were discussed and suggestions for further research were made.
Comparison of 2-step laparoscopy-guided abomasopexy versus omentopexy via right flank laparotomy for the treatment of dairy cows with left displacement of the abomasum in on-farm settings.

OBJECTIVE To compare characteristics and results of 2-step laparoscopy-guided abomasopexy versus omentopexy via laparotomy in the right paralumbar fossa for the treatment of dairy cows with a left displaced abomasum (LDA). DESIGN Prospective clinical trial. 253 dairy cows with an LDA. PROCEDURES All cows that were treated with omentopexy (n = 101) or 2-step laparoscopy-guided abomasopexy (152) from July 2005 through December 2006 were included. Presurgical, perisurgical, and postsurgical information was recorded by attending veterinarians. Producers were interviewed by telephone 7 and 60 days after surgery regarding the response of cows to surgery. Characteristics of and responses to the 2 surgical techniques were compared. RESULTS At 7 days after surgery, the 2 groups of cows were not significantly different with respect to appetite, comfort, and milk yield; at 60 days after surgery, groups were similar with respect to milk yield, cull rates, and risk of relapse of LDA. Antimicrobial treatment in response to postsurgical pyrexia was necessary in only 20.4% (31/152) of cows that were treated with 2-step laparoscopy-guided abomasopexy. Mean duration (preparation and surgery) of 2-step laparoscopy-guided abomasopexy was significantly less than that of omentopexy (36 vs 74 minutes, respectively). CONCLUSIONS AND CLINICAL RELEVANCE Results of 2-step laparoscopy-guided abomasopexy and omentopexy via laparotomy in the right paralumbar fossa were not significantly different. Compared with omentopexy, laparoscopy-guided abomasopexy was performed more quickly and required postsurgical administration of antimicrobials less frequently. Although these factors may be of economic consequence to veterinarians and producers, other aspects must also be considered when choosing between techniques.
The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species.

Uvomorulin belongs to the group of Ca2+‐dependent cell adhesion molecules, which are integral membrane proteins with several structural features in common. In particular, the cytoplasmic part of these proteins is highly conserved in different species, suggesting a common biological function. To test this assumption we transfected a uvomorulin full‐length cDNA into uvomorulin‐negative mouse NIH 3T3 and L cells. Immunoprecipitations with anti‐uvomorulin antibodies detected, in addition to uvomorulin, three independent proteins of 102, 88 and 80 kd which are of host origin and which form complexes with uvomorulin. Using cDNA constructs coding for uvomorulin with cytoplasmic or extracellular deletions it is shown that the 102, 88 and 80 kd proteins complex with the cytoplasmic domain of uvomorulin. Peptide pattern analysis revealed that these three proteins are identical in different mouse cells. When uvomorulin cDNA was introduced into cell lines from other species, such as human HeLa and avian fibroblasts, the expressed uvomorulin was also associated with endogenous 102, 88 and 80 kd proteins and, moreover, each of these proteins showed structural similarities to the respective mouse molecule. A panel of antibodies specific for known cytoplasmic proteins of mol. wts similar to those of the three proteins did not react with any of the described components. This suggests that the 102, 88 and 80 kd proteins constitute a new group of proteins for which we propose the nomenclature of catenin alpha, beta and gamma respectively. The characterization of these proteins provides a first molecular basis for a possible cytoplasmic anchorage of uvomorulin to the cytoskeleton.
Factor Structure and Internal Consistency of the Spanish Version of the Gordon Test of Visual Imagery Control

We examined the factor structure and internal consistency of the Spanish version of the Gordon Test of Visual Imagery Control, as well as the correlations with scores on the Vividness of Visual Imagery Questionnaire and the Verbalizer-Visualizer Questionnaire, for a sample of 479 undergraduates. Principal components analysis, followed by varimax orthogonal rotation, identified the expected four factors (Movement, Misfortune, Colour, and Stationarity), which jointly explained 55% of the variance. The Gordon Test had a Cronbach α value of . 69 and correlated significantly with scores on Marks' Vividness of Visual Imagery Questionnaire.
Tactile measuring systems for the recognition of unknown surfaces

A novel smart tactile sensor that recognizes the nature of the surfaces is presented. The approach is based on the idea of analyzing the signal produced when the sensor touches and stimulates the surface. An "intelligent probing" system for material recognition has been developed. It is based on the use of bimorph piezo-ceramic actuators and sensors that allow the unknown surface to be stimulated and the response signal sensed. Two different experimental prototypes of the tactile sensing system have been realized and their performance has been characterized. Several interesting applications have been considered with particular emphasis on the problems of "humanitarian demining" and automatic waste material recycling. Experimental results are given to show the efficiency of the smart measuring system.
Noninvasive biomarkers for the screening of fibrosis, steatosis and steatohepatitis in patients with metabolic risk factors: FibroTest-FibroMax experience.

Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic steatosis occurring in those who do not consume high amounts of alcohol. The prevalence of NAFLD is high in the general population with metabolic risk factors and insulin resistance being mainly associated with overweight, diabetes, hyperlipidemia and hypertension [1, 2]. Two histological patterns of NAFLD have been described: fatty liver alone and, in a minority of patients, necroinflammatory form of NAFLD known as non-alcoholic steatohepatitis (NASH) that may progress to cirrhosis and hepatocellular carcinoma [1]. Although confirmation of the diagnosis of NAFLD can be achieved by imaging methods, they do not provide data on staging the disease (steatohepatitis and fibrosis), which until recently required a liver biopsy for confirmation. Due to the high prevalence of NAFLD in at risk populations [1], the limitations of biopsy [3, 4] and the developing of reliable noninvasive blood tests [5-8], liver biopsy should no longer be considered mandatory as first-intention screening of liver lesions [9, 10].
Tetracyclines and Host-Defense Mechanisms

The effects of different tetracyclines on the bactericidal action of serum and on phagocytosis and intracellular killing of bacteria were investigated. Opsonization, phagocytosis, and intracellular killing were not significantly affected by any of the investigated tetracyclines. The bactericidal action of serum on Escherichia coli was substantially reduced by all tetracyclines investigated. In this respect, doxycycline had a much more pronounced action than the other tetracyclines. The serum bactericidal effect could be completely restored by addition of Mg2+.
Electrostimulating device in the management of xerostomia.

INTRODUCTION The present study was undertaken to evaluate the safety and effectiveness of a recently developed electrostimulating device mounted on an individualized intra-oral removable appliance. MATERIALS AND METHODS The device, containing electrodes, a wetness sensor, an electronic circuit and a power source, was tested on patients with xerostomia in a crossover, randomized, sham-controlled, double-blinded, multicenter study. Electrical stimulation and also sham were delivered during 10 min to the oral mucosa, in the mandibular third molar region. Oral dryness was measured by the sensor. As the primary outcome, sensor dryness and xerostomia symptom changes as a result of device wearing were assessed, and compared between active and sham modes. In addition, side-effects were recorded. RESULTS Electrostimulation resulted in a significant decrease in sensor dryness, leading to a beneficial effect on patients' subjective condition. No significant side-effects were observed.
Knowledge Sharing between Enterprises of the Same Group

This exploratory study seeks to promote investigation about knowledge sharing between enterprises of the same enterprise group and is motivated by the growing importance of knowledge management (KM) to businesses. The data was collected from Community Innovation Survey (CIS) 2012 and three linear regressions were performed (one to all enterprises, one to SME and one to large enterprises). The results show that cooperation for product or process innovation and service innovation influence knowledge sharing between enterprises of the same enterprise group, contributing to learning organizations. The present paper clarifies the importance of innovation and cooperation for innovation in KM practices between enterprises of the same enterprise group. The results are only valid to SME, since large enterprises have different practices of KM.
How people live.

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Agonising over ASILs: controllability and the in-wheel motor

In the automotive domain the standard ISO 26262 places significant emphasis on the assignment of Automotive Safety Integrity Levels (ASILs). In particular much of Part 3 of the standard is dedicated to the process that determines the three factors that contribute to the final assigned ASIL value: exposure, severity and controllability. In this paper we examine some of the issues that the authors have encountered during the development of an in-wheel electric motor and will argue that the perceived emphasis on ASIL ratings, in the context of developing a safe system, is misplaced and potentially counterproductive. (8 pages)
Human cytomegalovirus glycoprotein B genotypes in blood of AIDS patients: Lack of association with either the viral DNA load in leukocytes or presence of retinitis

It has been suggested that human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes could be used as a marker for viral virulence in patients with AIDS. The present study was designed to evaluate a possible association between specific gB genotypes, the presence of HCMV retinitis, and the HCMV viral load. Fifty‐four blood samples were obtained from 54 HIV‐ and HCMV‐infected patients. Twenty‐seven of these patients were asymptomatic for HCMV, whereas the other 27 patients had been diagnosed recently with HCMV retinitis. HCMV gB genotyping was carried out by using restriction enzyme analysis of PCR‐amplified PMNL extracts. Determination of the HCMV viral load in the same specimens was carried out using a quantitative‐PCR. HCMV gB genotype 2 was found more frequently than other genotypes in PCR‐amplified polymorphonuclear leukocytes (PMNL) of patients with AIDS (P < 0.05) but not more frequently in samples from patients with HCMV retinitis. No significant association was found between any HCMV gB genotypes and the viral load in blood. In conclusion, the actual HCMV gB genotyping system using PMNL provides no additional benefit over the viral load in blood for identification of HIV‐infected subjects at risk of HCMV disease. J. Med. Virol. 59:98–103, 1999. © 1999 Wiley‐Liss, Inc.
A Comparative Study of Coronary Risk Factors between Urban and Rural Diabetics in Gaza Strip = دراسة لمقارنة عوامل الخطر المؤدية لمرض شريان القلب التاجي عند مرضى السكري بين المنطقة الحضرية والمنطقة الريفية لقطاع غزة

Aim: The aim of this study is to compare the lipid profile levels and other CHD risk factors among DM patients in an urban region (Gaza governorate) with those of the rural region (the eastern region of Khanyounis Governorate) of Gaza Strip. Methods: The study is cross-sectional and included 200 DM patients, aged 10-65 years, whose diabetes was identified for at least two years. The samples of this study were taken from diabetic service units in the Palestinian Medical Relief Society, Gaza governorate and primary healthcare clinics, eastern region of Khanyounis. Personal and clinical data of the study subjects were collected using an interview questionnaire. Anthropometric evaluation were carried out. Serum was used to determine fasting blood glucose and lipid profiles automatically by chemistry spectrophotometer. Results: There was a statistically significant difference among the study population according to physical activity (P=0.004). Moreover, High levels of serum total cholesterol (TC) (≥240mg/dl) were seen in 39.0% of urban group and 23.0% of rural group. High low-density lipoprotein cholesterol (LDL-c) levels (≥160mg/dl) were observed in 15.0% of urban group and 8.0% of rural group. However, there was a statistically significant difference among the study population according to the total cholesterol and LDL levels (P=0.041and 0.002 respectively). On the other hand, there were no statistically significant differences among the study population according to triglycerides and HDL levels (P= 0.153 and 0.594 respectively).
Cell Phone Coverage and Traffi c Accidents : New Evidence using Cell Phone Towers

We examine the relationship between cell phone use and traffi c accidents using a unique measure of cell phone coverage. The distance from an antenna tower accurately predicts whether or not an area has cellular coverage according to provider coverage maps in 2016. Cellular coverage between 2001 and 2011 is proxied for using the the distance between a road segment and the nearest antenna tower. Empirical results show that when an antenna tower is constructed closer to a road segment, there is no change in the total number of automobile accidents. However, when towers are constructed closer to a road segment, there is a significant decrease in alcohol-related accidents in more rural areas. In urban areas, tower construction is associated with an increase in accidents caused by a distraction.
Single Intraoperative Application of 5-Fluorouracil Versus Placebo In Low-Risk Trabeculectomy Surgery: A Randomized Trial

PurposeTrabeculectomy is the procedure of choice for the surgical control of glaucoma, and is often augmented by intraoperative 5-fluorouracil application to reduce scarring. This study aims to assess the validity of this technique with a randomized placebo-controlled trial. MethodsPatients without risk factors for bleb failure other than previous drop use who were undergoing trabeculectomy surgery as their first ocular procedure were randomized to receive fluorouracil or placebo. The main outcome measures were intraocular pressure and bleb morphology. ResultsResults from 40 eyes of 36 patients are presented; 23 eyes were treated with fluorouracil, and 17 eyes were treated with placebo. Mean intraocular pressure at 1 year and at the final follow-up examination was significantly lower than preoperatively, but was not different between fluorouracil and placebo groups. Kaplan-Meier survival analysis also failed to show any difference, though there was a trend toward better survival of an intraocular pressure of 16 mm Hg or lower. Bleb morphology was examined postoperatively in 24 patients, with no differences found between fluorouracil and placebo groups. ConclusionsNo significant difference was found in trabeculectomy outcome between fluorouracil-treated and placebo-treated eyes. The study was too small to state definitively that no such difference exists, but suggests that any treatment effect is likely to be small.
Large-Scale Organizational and Managerial Change in Health Care: A Review of the Literature

This paper takes an overview of the organizational and managerial literature on recent large-scale change efforts within health care organizations. Such literature refers to issues of enhanced policy significance, as a succession of such changes has swept through health care, at an international level. Interpretive and case study method have been widely employed in this field. While the literature is emergent, key empirical concerns can be identified: (1) Changing roles and relationships, with the rise of management and the challenge to clinical domination; some argue that radical deprofessionalization now is evident, while others take a more nuanced view. (2) The impact of marketization, with health care becoming more of a commodity; various models of a health care ‘quasi market’ have been formulated. (3) Understanding the process of change in health care organizations, such as the development of a management of change literature. New theoretical frameworks have been developed, notably ‘the reform cycle’ as a way of understanding progressive cycles of organizational reform, the impact on health care of the rise of the new public management, and examining the demedicalization thesis through the more generic literature on professions. The paper concludes with a discussion of what this research base could contribute to policy-making.
From image processing to classification: I. Modeling disturbances of isoelectric focusing patterns

In order to optimize the conditions for evaluation of isoelectric focusing (IEF) patterns by digital image processing, the sources of error in determination of the pI values were analyzed together with the influence of a varying background. The effects of band distortions, in the spectra of the individual lanes, were examined. In order to minimize the effect of these distortions, optimal conditions for handling IEF patterns by digital image processing were elucidated. The systematic part of the global deformation on the gels was investigated and an algorithm was developed by which it was possible to correct for a part of the individual distortions. The effects of various corrections for lane distortions were illustrated by classification, using different types of discriminant analysis. Finally the background disturbances were examined, and described by a mathematical model.
Interleukin-12 inhibits hepatitis B virus replication in transgenic mice

Interleukin-12 (IL-12) is a heterodimeric cytokine produced by antigen-presenting cells that has the ability to induce gamma interferon (IFN-gamma) secretion by T and natural killer cells and to generate normal Th1 responses. These properties suggest that IL-12 may play an important role in the immune response to many viruses, including hepatitis B virus (HBV). Recently, we have shown that HBV-specific cytotoxic T lymphocytes inhibit HBV replication in the livers of transgenic mice by a noncytolytic process that is mediated in part by IFN-gamma. In the current study, we demonstrated that the same antiviral response can be initiated by recombinant murine IL-12 and we showed that the antiviral effect of IL-12 extends to extrahepatic sites such as the kidney. Southern blot analyses revealed the complete disappearance of HBV replicative intermediates from liver and kidney tissues at IL-12 doses that induce little or no inflammation in these tissues. In addition, immunohistochemical analysis demonstrated the disappearance of cytoplasmic hepatitis B core antigen from both tissues after IL-12 treatment, suggesting that IL-12 either prevents the assembly or triggers the degradation of the nucleocapsid particles within which HBV replication occurs. Importantly, we demonstrated that although IFN-gamma, tumor necrosis factor alpha, and IFN-alpha/beta mRNA are induced in the liver and kidney after IL-12 administration, the antiviral effect of IL-12 is mediated principally by its ability to induce IFN-gamma production in this model. These results suggest that IL-12, through its ability to induce IFN-gamma, probably plays an important role in the antiviral immune response to HBV during natural infection. Further, since relatively nontoxic doses of recombinant IL-12 profoundly inhibit HBV replication in the liver and extrahepatic sites in this model, IL-12 may have therapeutic value as an antiviral agent for the treatment of chronic HBV infection.
Night eating and obesity in the EP3R-deficient mouse

Adult mice carrying a null mutation of the prostanoid receptor EP3R (EP3R−/− mice) exhibit increased frequency of feeding during the light cycle of the day and develop an obese phenotype under a normal fat diet fed ad libitum. EP3R−/− mice show increased motor activity, which is not sufficient to offset the increased feeding leading to increased body weight. Altered “nocturnal” activity and feeding behavior is present from a very early age and does not seem to require age-dependent factors for the development of obesity. Obesity in EP3R−/− mice is characterized by elevated leptin and insulin levels and >20% higher body weight compared with WT littermates. Abdominal and subcutaneous fat and increased liver weight account for the weight increase in EP3R−/− mice. These observations expand the roles of prostaglandin E2 signaling in metabolic regulation beyond the reported stimulation of leptin release from adipose tissue to involve actions mediated by EP3R in the regulation of sleep architecture and feeding behavior. The findings add to the growing literature on links between inflammatory signaling and obesity.