Miracle-dz/UniFAR-FTUs huggingface dataset:
Spinal and Supraspinal Control of the Direction of Stepping during Locomotion

Most bipeds and quadrupeds, in addition to forward walking, are also capable of backward and sideward walking. The direction of walking is determined by the direction of stepping movements of individual limbs in relation to the front-to-rear body axis. Our goal was to assess the functional organization of the system controlling the direction of stepping. Experiments were performed on decerebrate cats walking on the treadmill with their hindlimbs, whereas the head and trunk were rigidly fixed. Different directions of the treadmill motion relative to the body axis were used (0, ±45, ±90, and 180°). For each direction, we compared locomotion evoked from the brainstem (by stimulation of the mesencephalic locomotor region, MLR) with locomotion evoked by epidural stimulation of the spinal cord (SC). It was found that SC stimulation evoked well coordinated stepping movements at different treadmill directions. The direction of steps was opposite to the treadmill motion, suggesting that this direction was determined by sensory input from the limb during stance. Thus, SC stimulation activates limb controllers, which are able to generate stepping movements in different directions. By contrast, MLR stimulation evoked well coordinated stepping movements only if the treadmill was moving in the front-to-rear direction. One can conclude that supraspinal commands (caused by MLR stimulation) select one of the numerous forms of operation of the spinal limb controllers, namely, the forward walking. The MLR can thus be considered as a command center for forward locomotion, which is the main form of progression in bipeds and quadrupeds.
Coffee resistance to the main diseases: leaf rust and coffee berry disease

Considerable success has been obtained in the use of classical breeding to control economically important plant diseases, such as the coffee leaf rust and the coffee berry disease (CBD). There is a strong consensus that growing genetically resistant varieties is the most appropriate cost effective means of managing plant diseases and is one of the key components of crop improvement. It has also been recognized that a better knowledge of both, the pathogens and the plant defence mechanisms will allow the development of novel approaches to enhance the durability of resistance. After a brief description of concepts in the field of plant disease resistance, we attempt to give a view of the research progress on coffee leaf rust and CBD concerned with the pathogens infection and variability, coffee breeding for resistance and coffee resistance mechanisms.
HERBAL SUPPLEMENTS: A POTENTIAL FOR DRUG INTERACTIONS IN TRANSPLANT RECIPIENTS

Background. Herbal dietary supplements represent a potential and possibly an overlooked cause for drug interactions in transplant recipients. Methods. Two patients are reported which suggest that St. John’s Wort (SJW) may induce cytochrome P-450 3A4 activity and/or P-glycoprotein expression. Both of these mechanisms are significantly involved in the metabolism and absorption of cyclosporine (CSA) and other immunosuppressants. Results. After two renal transplant recipients started self-medicating with SJW, their CSA concentrations were consistently documented to be subtherapeutic. While on SJW, one patient developed acute graft rejection due to low CSA concentrations. In both patients, termination of SJW returned their CSA concentrations to therapeutic values. Conclusions. Patients taking SJW concomitantly with CSA or other medications whose absorption and metabolism are mediated by cytochrome P-450 and/or P-glycoprotein should require close monitoring. Potential herb-prescription drug interactions are not just limited to SJW. Inquiries regarding the usage of herbal supplements should be an integral component of a transplant recipient’s medication history.
Radiological imaging in pneumonia: recent innovations

Purpose of review Pneumonia is one of the major infectious diseases responsible for significant morbidity and mortality throughout the world. Radiological imaging plays a prominent role in the evaluation and treatment of patients with pneumonia. This paper reviews recent innovations in the radiologic diagnosis and management of suspected pulmonary infections. Recent findings Chest radiography is the most commonly used imaging tool in pneumonias because of availability and an excellent cost–benefit ratio. Computed tomography is mandatory in unresolved cases or when complications of pneumonia are suspected. A specific radiologic pattern can suggest a diagnosis in many cases. Bacterial pneumonias are classified into four main groups: community-acquired, aspiration, healthcare-associated and hospital-acquired pneumonia. The radiographic patterns of community-acquired pneumonia may be variable and are often related to the causative agent. Aspiration pneumonia involves the lower lobes with bilateral multicentric opacities. The radiographic patterns of healthcare-associated and hospital-acquired pneumonia are variable, most commonly showing diffuse multifocal involvement and pleural effusion. Summary Combination of pattern recognition with knowledge of the clinical setting is the best approach to the radiologic interpretation of pneumonia. Radiological imaging will narrow the differential diagnosis of direct additional diagnostic measures and serve as an ideal tool for follow-up examinations.
Mycosis fungoides with extracutaneous dissemination: A distinct clinicopathologic entity

The clinical and histopathologic findings in 15 autopsied cases of mycosis fungoides with extracutaneous dissemination were reviewed. Mycosis fungoides involvement of the skin was present for an average of 22 months (range of 7 months to 6 years) from the time of diagnostic skin biopsy until the onset of clinical signs indicating systemic involvement. The clinical findings associated with dissemination of mycosis fungoides were fever, weight loss, generalized peripheral and hilar lymphadenopathy, palpable enlargement of the liver and spleen, and peripheral blood eosinophilia and lymphocytosis. The average survival from the development of these signs was 11 months (range of 3 to 18 months). At autopsy in all 15 cases cutaneous mycosis fungoides was associated with malignant lymphoma, mycosis fungoides type, involving lymphoid organs and viscera. The cellular infiltrates within extracutaneous sites of involvement were similar to those of the skin infiltrates and included variable numbers of pleomorphic mycosis cells admixed with many inflammatory cells. In none of the cases was there evidence of transition to a malignant lymphoma of a different cell type. Extracutaneous dissemination is a clinicopathologically distinct aspect of the natural history of mycosis fungoides and is not due to the development of a different type of malignant lymphoma.
Triamcinolone acetonide decreases outflow facility in C57BL/6 mouse eyes.

PURPOSE To determine the effect of triamcinolone acetonide (TA) on outflow facility in mice. METHODS Animals received 20 μL of TA (40 mg/mL) suspension subconjunctivally either bilaterally or unilaterally and were euthanized after either 1 week or 3 weeks. Before mice were killed, IOP was measured with a rebound tonometer. Outflow facility was determined using simultaneous pressure and flow measurements. Another set of animals received bilateral injection of anecortave acetate (AA) with or without bilateral TA injection and their outflow facility was also determined. Myocilin expression was investigated in a subset of eyes using quantitative PCR (qPCR). RESULTS Outflow facility of eyes in animals receiving bilateral TA injection (TA(BL)) and TA-treated eyes of animals receiving unilateral injection (TA(UL)) was significantly decreased compared to naïve control eyes (C(naive)) after 1 week and 3 weeks of TA treatment (ANOVA P < 0.01, P < 0.001, respectively). Eyes treated with AA (with or without TA) had higher outflow facility than animals treated with TA (P < 0.05). IOP data did not show any significant difference between groups. qPCR analysis revealed significant decrease in myocilin expression in eyes receiving AA compared to naïve control and TA-treated eyes (ANOVA P < 0.001). CONCLUSIONS Steroid treatment significantly decreases outflow facility in C57BL/6 mice despite having small effect on IOP. This animal model can be useful for studying the pathogenesis of steroid-induced glaucoma.
[Regulation of energy metabolism by bone].

In terms of feedback regulation in Endocrinology, one question was arisen from the evidences that energy metabolism regulates bone metabolism. "Does bone metabolism regulates energy metabolism? " To address this question, Dr. Gerard Karsenty and his colleagues studied energy metabolism in the mice lacking Osteocalcin, an osteoblast-specific gene. The mutant mice exhibited abnormal energy metabolism phenotypes, diabetes and obesity. Here I would like to remind you his previous and recent works so that we can discuss how to apply bone metabolism information to therapeutic strategy for metabolic syndrome.
Spectral sensitivity of the feedback signal from horizontal cells to cones in goldfish retina

The spectral sensitivity of cones in isolated goldfish retina was determined with whole-cell recording techniques. Three spectral classes of cones were found with maximal sensitivities around 620 nm, 540 nm, and 460 nm. UV-cones were not found because our stimulator did not allow effective stimulation in the UV range. The spectral sensitivity of the cones closely matched the cone photopigment absorption spectra at the long wavelength side of the spectrum, but deviated significantly at shorter wavelengths. Surround stimulation induced an inward current in cones due to feedback from horizontal cells. The spectral sensitivity of this feedback signal was determined in all three cone classes and found to be broader than the spectral sensitivity of the cones recorded from, and to be spectrally nonopponent. These data are consistent with a connectivity scheme between cones and horizontal cells in which the three horizontal cell systems feed back to all cone systems and in which all horizontal cell systems receive input from more than one cone system.
Incidence and pattern of varicocele recurrence after laparoscopic ligation of the internal spermatic vein with preservation of the testicular artery.

OBJECTIVES To determine the incidence and pattern of recurrence of varicocele after laparoscopic internal spermatic vein ligation with testicular artery preservation. METHODS In a prospective study, 16 patients who underwent 20 laparoscopic varix ligation were evaluated postoperatively for recurrence by clinical physical examination and percutaneous spermatic venography. RESULTS No significant complications were encountered with the surgical or radiological procedure. Clinical recurrence was detected by physical examination in 20% of cases, while percutaneous spermatic venography detected recurrence in 45% of cases. The sensitivity and specificity of clinical physical examination for detecting varicocele recurrence was 33% and 90.9%, respectively with an accuracy rate of 65%. Recurrences were through parallel collaterals or medial transverse collaterals in 88.8% and 11.2%, respectively. Parallel collaterals joined the spermatic vein in mid or high retroperitoneum in seven patients while it joined the renal vein in one patient. There were no low retroperitoneal parallel collaterals. CONCLUSIONS Laparoscopic ligation of internal spermatic vein with preservation of testicular artery is a procedure that is associated with low morbidity and quick recovery. It is able to achieve its surgical objective in only 55% of cases, however. Such information should be taken into consideration during patient counselling when selecting the operative technique of choice for varicocele ligation.
uPA‐derived peptide, Å6 is involved in the suppression of lipopolysaccaride‐promoted inflammatory osteoclastogenesis and the resultant bone loss

Chronic inflammatory diseases such as rheumatoid arthritis and periodontitis frequently cause bone destruction. Inflammation‐induced bone loss results from the increase of bone‐resorbing osteoclasts. Recently, we demonstrated that urokinase type plasminogen activator (uPA) suppressed lipopolysaccaride (LPS)‐inflammatory osteoclastogenesis through the adenosine monophosphate‐activated protein kinase (AMPK) pathway, whereas its receptor (uPAR) promoted that through the Akt pathway.
Foraging and Nesting Behavior of Osmia lignaria (Hymenoptera: Megachilidae) in the Presence of Fungicides: Cage Studies

Abstract During orchard pollination studies in California, we observed dramatic changes in nesting and foraging behavior of Osmia lignaria Say (Hymenoptera: Megachilidae) after sprays with tank mixtures containing fungicides. A characteristic pattern of postspray events observed includes erratic behavior and interrupted foraging and nesting activity for several days. In an effort to determine whether fungicidal sprays were disruptive to bee foraging and thus to pollination, we exposed O. lignaria females nesting in field cages planted with lacy scorpionweed, Phacelia tanacetifolia Benth (Hydrophyllaceae), to selected spray mixtures normally encountered in California orchard production systems: iprodione (Rovral), propiconazole (Orbit), benomyl (Benlate), and captan (Captan 50 WP); the surfactant Dyne-Amic, alone and mixed with Rovral; and the tank mixture IDB (Rovral + Dyne-Amic + the foliar fertilizer Bayfolan Plus). An additional cage sprayed with an equal volume of water acted as control, and a cage sprayed with the insecticide dimethoate as a toxic standard. For each female O. lignaria, we recorded time spent inside the nest depositing pollen-nectar loads, foraging time, cell production rate, and survival. All females in the dimethoate treatment died postspray + 1 d. Before death, some of these females behaved similarly to our previous orchard observations. A high proportion of females in the IDB cage were inactive for a few hours before resuming normal foraging and nesting activity. No lethal or behavioral effects were found for any of the other compounds or mixtures tested. Our results indicate that the fungicide applications that we tested are compatible with the use of O. lignaria as an orchard pollinator.
Evaluation of risk and diagnostic value of quantitative assays for anti-Toxoplasma gondii immunoglobulin A (IgA), IgE, and IgM and analytical study of specific IgG in immunodeficient patients

To determine their prognostic and diagnostic values for toxoplasmosis in immunodepressed subjects, we assayed immunoglobulin A (IgA) and IgE antibodies by means of immunocapture (IC) tests, with revelation done by using a suspension of T. gondii (ICT). We also carried out a simultaneous analytical study of IgG antibodies on cellulose acetate membranes by using the comparative immunological profile method and an enzyme-linked immunofiltration assay (ELIFA). A total of 1,238 samples (serum, cerebrospinal fluid, and aqueous humor from 318 patients) were tested. IgA and IgE antibodies were detected in all heart, kidney, and liver transplant recipients with clinical manifestations of toxoplasmosis; IgA was detected in the aqueous humor of a patient with chorioretinitis. In patients with AIDS-related toxoplasmosis, including the cerebral form, IgA and IgE antibodies or a significant modification of ELIFA IgG values were observed in 38, 19, and 25% of patients, respectively. IgM was detected by ICT only in 12% of patients and aided the diagnosis in 1 of 71 patients. IC tests for specific IgA and IgE alone and combined with ELIFA were positive in 39 and 46% of patients who developed clinical toxoplasmosis, respectively. In a serial study of 16 patients in whom at least one of these three tests was positive, a significant immunological signal sometimes preceded clinical onset by 1, 6, and even 17 months. Similarly, in a group of human immunodeficiency virus-infected patients with evidence of previous exposure to T. gondii but no clinical manifestations, IgA, IgE, and IgA and/or IgE antibodies were detected in only 11, 4, and 12% of patients, respectively. These two situations point to peripheral T. gondii reactivation. IgA and IgE emerged as interesting markers of the risk of toxoplasmosis in immunodepressed patients. They may also provide valuable assistance in the diagnosis of toxoplasmosis, especially because tests for specific IgM are disappointing. However, at least one in two patients with toxoplasmosis showed no detectable immunological reaction, suggesting that this polyisotypic approach should be combined with other noninvasive methods such as gene amplification.
A Parallel Estimator with LMS Adaptation for Fast Fading Channels

We propose a novel channel estimation technique for fast fading channels. The proposed scheme supposes multiple linear transversal filters, which are constructed in a parallel structure. The estimator coefficients for the proposed estimation scheme are adapted by the least mean square (LMS) algorithm. To obtain a further performance improvement of the proposed scheme, a technique to adjust the step-size for the LMS is also introduced. Computer simulation results show that the proposed method provides a significant improvement related to the conventional LMS estimator in high fade rate conditions.
Detection and Tele-replication of Human Hand Motions by a Robotic Hand

This paper presents a tele-operated robotic hand controlled by replication of human hand motions and is focused on the description of technical solutions for detection and tele-replication of movements, in order to control a robotic hand. The purpose of such research is justified by the need of high precision human controlled operations in special environments. The system is based on a flex sensors set with processing units and has as effectors a robotic arm and an anthropomorphic hand. The current article displays the modality of achieving an anthropomorphic robotic arm capable of efficiently handling objects of different sizes. In order to implement and test the technical and computing solutions, the authors have used a commercial product as experimental platform and improved it both in its mechanical structure and in its command and control system. For implementing the motion algorithms of the robotic arm, a method was developed for decoding arm movements performed by a human operator. To this end, bending sensors placed at the human operator’s joints (shoulder, elbow, wrist and fingers) were used. Signals collected from the sensors during the realization of these different movements by the human operator were decoded, processed and implemented in the drive system corresponding to the anthropomorphic robotic arm. In this regard, all sets of complex movements by the human arm operator were duplicated and implemented in the anthropomorphic robotic arm. The results obtained in handling various objects by means of using the anthropomorphic robotic arm have certified the effectiveness of this method.
Relational governance – an examination of the apple sector in Albania

This study provides an in-depth analysis of the value chain governance in the fruit sector in Albania. Particular attention was paid to the nature of relations between agricultural producers and marketing channel chosen by them, distinguishing between big wholesalers, local collectors and exporters, longer-term relations, commitment and trust, and contracting along the value chain. Given the absence of, or weak, contract enforcement and the clear evidence of embeddedness in dyadic relationships in this chain, we focused our analysis on sustained relationships, contracting and personal bonds as a determinant factor to improve business relationship quality. This paper is based on a structured survey targeting 182 orchard farmers in two leading fruit production regions in Albania, namely Korca and Peshkopia. The survey showed a weak prevalence of verbal contracts in embedded relationships. The percentage of contracts between farmers and local collectors is slightly higher compared to wholesalers. This evidence is much stronger when we investigate the incidence of contracts in stable relationships (i.e. selling to the same buyer). We also find that mature relationships generate higher sustainability - a result which does not come as a surprise, although the coordination between farmers and other actors downstream is only at early stages. The main implications which arise from our analysis are relevant for agribusinesses, farmers and policy makers.
Mesenchymal Stem Cell Differentiation into Adipocytes Is Equally Induced by Insulin and Proinsulin In Vitro

Background and Objectives In advanced β-cell dysfunction, proinsulin is increasingly replacing insulin as major component of the secretion product. It has been speculated that proinsulin has at least the same adipogenic potency than insulin, leading to an increased tendency of lipid tissue formation in patients with late stage β-cell dysfunction. Methods and Results Mesenchymal stem cells obtained from liposuction material were grown in differentiation media containing insulin (0.01 μmol), proinsulin (0.01 μmol) or insulin+proinsulin (each 0.005 μmol). Cell culture supernatants were taken from these experiments and an untreated control at weeks 1, 2, and 3, and were stored at −80°C until analysis. Cell differentiation was microscopically supervised and adiponectin concentrations were measured as marker for differentiation into mature lipid cells. This experiment was repeated three times. No growth of lipid cells and no change in adiponectin values was observed in the negative control group (after 7/14/12 days: 3.2±0.5/3.3±0.1/4.4±0.5 ng/ml/12 h). A continuous differentiation into mature adipocytes (also confirmed by Red-Oil-staining) and a corresponding increase in adiponectin values was observed in the experiments with insulin (3.6±1.9/5.1±1.4/13.3±1.5 ng/ml/12 h; p<0.05 week 1 vs. week 3) and proinsulin (3.3±1.2/3.5±0.3/12.2±1.2 ng/ml/12 h; p<0.05). Comparable effects were seen with the insulin/proinsulin combination. Conclusions Proinsulin has the same adipogenic potential than insulin in vitro. Proinsulin has only 10~20% of the glucose-lowering effect of insulin. It can be speculated that the adipogenic potential of proinsulin may be a large contributor to the increased body weight problems in patients with type 2 diabetes and advanced β-cell dysfunction.
Fault Estimation for Single Output Nonlinear Systems Using an Adaptive Sliding Mode Observer

Abstract In this paper, a class of single output nonlinear systems with an uncertain parameter is considered. A diffeomorphism is first introduced to simplify the system structure, then, by employing an adaptive approach to identify the unknown parameter, a sliding mode observer is developed to estimate the system state variables. Based on the observer, a fault estimation scheme is proposed based on the minimisation of a weighted ℒ 2 norm of the fault estimation error. A simulation example is given to demonstrate the proposed scheme.