Miracle-dz/UniFAR-FTUs huggingface dataset:
Degradation of chloroaromatics: purification and characterization of maleylacetate reductase from Pseudomonas sp. strain B13

Maleylacetate reductase of Pseudomonas sp. strain B13 was purified to homogeneity by chromatography on DEAE-cellulose, Butyl-Sepharose, Blue-Sepharose, and Sephacryl S100. The final preparation gave a single band by polyacrylamide gel electrophoresis under denaturing conditions and a single symmetrical peak by gel filtration under nondenaturing conditions. The subunit M(r) value was 37,000 (determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Estimation of the native M(r) value by gel filtration gave a value of 74,000 with a Superose 6 column, indicating that the enzyme is dimeric. The pH and temperature optima were 5.4 and 50 degrees C, respectively. The pI of the enzyme was estimated to be 7.0. The apparent Km values for maleylacetate and NADH were 58 and 30 microM, respectively, and the maximum velocity was 832 U/mg of protein for maleylacetate. Maleylacetate and various substituted maleylacetates, such as 2-chloro- and 2-methyl-maleylacetate, were reduced at significant rates. NADPH was also used as a cofactor instead of NADH with nearly the same Vmax value, but its Km value was estimated to be 77 microM. Reductase activity was inhibited by a range of thiol-blocking reagents. The absorption spectrum indicated that there was no bound cofactor or prosthetic group in the enzyme.
A comparison of strengths of five core and post-and-core systems.

OBJECTIVE In this in vitro study, the strength of several core and post-and-core systems was compared. A second aim was to compare the strength of posts and cores on root-filled teeth with that of cores on vital teeth. The failure modes of the core and post-and-core systems were also studied. METHOD AND MATERIALS For root-filled teeth, Composipost carbon fiber dowels and gold alloy posts and cores were tested. For vital teeth, glass-ionomer cement with threaded parapulpal retention pins, resin composite with threaded parapulpal retention pins, and gold alloy with parallel parapulpal pins were tested. Specimens were tested in a Zwick universal material-testing machine. RESULTS A significant variation in strength was found among core systems of cast gold, resin composite, and glass-ionomer cement constructed on vital teeth. The strength of the systems constructed on root-filled teeth did not vary significantly. The mode of failure varied, depending on the core or post-and-core material. CONCLUSION Composipost posts and cores and cast gold posts and cores were equivalent in strength and did not vary significantly from gold cores constructed on vital teeth. The low strength values obtained for glass-ionomer cement in combination with threaded retention pins makes this combination a poor choice for core buildup.
Aphasia rehabilitation and the role of computer technology: Can we keep up with modern times?

Numerous computer applications have been developed specifically for aphasia rehabilitation. In this paper, the role of these computer programs is discussed in relation to three complementary treatment approaches in aphasia rehabilitation: disorder-oriented treatment, functional treatment, and participation-oriented treatment. Most of the programs available focus on disorder-oriented treatment and several studies have reported a beneficial effect on language skills. Nowadays, in the context of disorder-oriented treatment, these applications are indispensible to achieve an adequate treatment frequency of at least 2 hours per week. Computer applications aiming at functional and social participation goals are less well-developed. Several studies show that high-technology AAC can be used to support off-line communication. Moreover, it is reported that the AAC training has a positive effect on overall communicative functioning. In the near future, computer applications for interactive communicative training may become an important tool in aphasia rehabilitation. Theoretically, the internet offers excellent opportunities to improve social participation for people with aphasia, but reading and writing problems limit their access to the internet. So far, only a few initiatives have been reported to support and increase their access.
Distribution and determinants of personal exposure to nitrogen dioxide in school children.

OBJECTIVES: To assess the distribution of personal exposures to nitrogen dioxide (NO2) in school children, and to investigate factors that might influence personal exposure. METHODS: NO2 exposures were assessed by use of passive diffusion tubes for 46 children aged 9-11 years, selected from two Southampton schools. The tubes were worn for seven days, and parallel measurements were made with static samplers in the child's kitchen, living room, classroom, and playground. Information about potential exposures was collected by questionnaire. RESULTS: Personal exposures to NO2, averaged over seven days, ranged from 11 to 257 micrograms/m3 (6 to 137 ppb) with a geometric mean of 36 micrograms/m3 (19 ppb). Exposures correlated with concentrations of NO2 recorded in the home, but the relation was far from exact. Factors associated with increased personal exposure included the use of gas appliances in the home, living with one or more smokers, and travel to school by means other than a car. However, together these variables only explained a small part of the variation in personal exposures. CONCLUSIONS: These findings reinforce the need for personal monitoring of exposure in studies investigating potential health effects of NO2 in children.
Private Sector in Indian Healthcare Delivery: Consumer Perspective and Government Policies to promote private Sector

This research paper attempts to collate literature from various sources, in an attempt to answer three pertinent questions related to healthcare in India. Firstly, what is it meant by ‘private sector’ in healthcare delivery system of India, secondly how has the private sector evolved over the decades and what has been the role of the government in propelling the growth. Finally, the paper tries to highlight some of the factors that have promoted the growth of private sector in India with specific reference to quality of medical care. The paper explicitly indicates that the deficiencies in the public health delivery system of India, was the key to growth of private infrastructure in healthcare. The shift of hospital industry for ‘welfare orientation’ to ‘business orientation’ was marked by the advent of corporate hospitals, supported by various policy level initiatives made by the government. Today, there are over 20 international healthcare brands in India with several corporate hospitals. However, a large section of the ‘private healthcare delivery segment’ is scattered and quality of medical care continues to remain a matter of concern. This paper tracks the various government initiatives to promote private investment in healthcare and attempts to explore the reasons for preference of the private sector. Surprisingly, in contrast to contemporary belief, quality of medical care doesn’t seem to be the leading cause for preference of the private sector. Except for a few select corporate and trust hospitals, quality of medical care in private sector seems to be poor and at times compromised.
Development of a silver in situ hybridisation based assay for the determination of ploidy status in molar pregnancy diagnosis

Summary The aim of this study was to establish a scoring method for ploidy analysis using silver in situ hybridisation (SISH) with a chromosome 17 centromere probe. SISH was performed using the Ventana chromosome 17 centromere probe on sections from formalin fixed, paraffin embedded archival cases of complete hydatidiform moles, partial hydatidiform moles and hydropic products of conception with previously established ploidy status (determined by flow cytometry or karyotyping). In order to determine ploidy status, a scoring method was developed based on both the average number of signals per nucleus (ASN) and the percentage of nuclei with three signals (N3S), enumerated in 50 villous cytotrophoblastic and/or stromal cells. The results of four independent observers were compared individually and collectively with previously established ploidy status. There was a highly statistically significant difference between diploid and triploid gestations for ASN (1.86 ± 0.13 and 2.70 ± 0.16 respectively, Student t-test, p < 0.0001) and for N3S (1.14 ± 1.65 and 71.59 ± 14.25 respectively, Student t-test, p < 0.0001). The sensitivity and specificity of the SISH-based assay was 99.1% and 100% respectively for ASN, and 100% and 100% respectively for N3S. A chromosome 17 centromere probe SISH-based assay can reliably distinguish between diploid and triploid gestations. This test has diagnostic utility in distinguishing partial hydatidiform moles from histological mimics.
ACCESS TO CREDIT: IMPLICATION FOR SUSTAINABLE RICE PRODUCTION IN NIGERIA

Nigeria spends huge sums of money annually on the importation of rice. Rice production requires some level of capital outlay. Most of the Nigerian farmers have no take-off capital and access to credit is usually very limited. This study investigated the effect of access to credit on rice production in Nigeria. A total of 208 rice farmers were sampled in Niger State, North-central Nigeria. Data collected were analyzed with the aid of descriptive statistics, t-test of difference of two means, Logit regression model, the Ordinary Least Square Regression model and the Chow test. Male within the economically active age dominated rice production enterprise in the study area. They kept large household size of about 16 people. Few of the farmers belong to cooperative societies and relied more on family labour to carry out farm activities. Farmers who had access to credit recorded higher yield and higher profit which may support economic sustainability of the enterprise. The Logit model result showed that age, education, gender and contact with extension services were the significant factors affecting the likelihood to have access to credit for rice production. Majority of the farmers got loans from microfinance bank and cooperative societies. The study recommended among others that disbursement of loan meant for agricultural sectors should be monitored and its uses properly supervised while more of such loans were made available to genuine rice farmers in order to achieve economically sustainable rice production in the country.
Subcapsular Hematoma and Hypertension Following Percutaneous Needle Biopsy of a Transplanted Kidney

Percutaneous needle biopsy of a transplanted kidney is indicated as a diagnostic procedure in the evaluation of unexplained deteriorated renal function after renal transplantation. This procedure is not always without complications. We report a case of a subcapsular hematoma and hypertension following a percutaneous needle biopsy of a transplanted kidney. The cause of hypertension in this patient and the management of the subcapsular hematoma are discussed.
Use of and beliefs about baseline photography in the management of patients with pigmented lesions: a survey of dermatology residency programmes in the United States

Medical photography is often used in dermatology to aid in the clinical surveillance of patients with pigmented lesions. This study aimed to assess the utilization, logistics, rationale and beliefs about the usefulness of baseline photography in patients with pigmented lesions by physicians in dermatology residency programmes, and to compare current utilization to that reported in the last decade. Questionnaires were mailed to directors of all accredited dermatology residency programmes in the United States (n = 105). Eighty-three physicians responded to the questionnaire (79%). Utilization of total body and individual lesion photography was reported by 63% and 75% of the respondents, respectively; 16% of the respondents did not use any method of photography. Reasons for using photography included the following beliefs: that it helps detect early melanoma, it results in fewer biopsies, and it reduces patient anxiety. Financial and logistical constraints were reasons why some programmes were not utilizing photography. Thus baseline photography is currently used in a majority of academic dermatology programmes as an aid in the early detection of melanoma.
Growth and Survival of Immature Haematobia irritans (Diptera: Muscidae) is Influenced by Bacteria Isolated from Cattle Manure and Conspecific Larvae

Abstract Twenty species of bacteria were isolated from cattle manure and seven species were isolated from the gut of larval horn fly Hematobia irritans (L.). Bacteria in manure belonged to the Bacillaceae, Pseudomonadaceae, Micrococcaceae, Corynebacteriaceae, Enterobacteriaceae, Microbacteriaceae, and two unassigned genera. Gut bacteria belonged to the Enterobacteriaceae, Bacillaceae, Neisseriaceae, and Pseudomonadaceae. H. irritans larval survival and growth on the various bacterial species were evaluated by rearing larvae in sterilized cattle manure that was inoculated with single bacterial isolates. H. irritans larvae failed to develop in sterilized, uninoculated manure, indicating that bacteria are necessary for larval development. Survival averaged 74% in nonsterilized manure and ranged from 4 to 53% in manure with individual isolates. Survival was highest when larvae were reared on manure inoculated with Pseudomonadaceae, Corynebacteriaceae, Micrococcaceae, and Bacillaceae and was lowest when reared in manure inoculated with Enterobacteriaceae and Microbacteriaceae. Pupal weights were heaviest when reared on the Flavobacteria, followed by the Pseudomonadaceae and Corynebacteriaceae. Pupae averaged 4.9 ± 0.08 mg when reared on gram-negative isolates, compared with 3.6 ± 0.09 mg when reared on gram-positive isolates. Pupal weights were not significantly correlated with larval survival, indicating that bacteria that promote growth do not necessarily promote survival. A reproductive index was used as a measure of fitness and was highest for larvae reared in the nonsterile control, followed most closely by Pseudomonadaceae and Corynebacteriaceae. These groups appeared to best meet the nutritional requirements of larvae and may be used in further experiments to define an artificial rearing media for H. irritans.
Demand for the Immediacy of Execution: Time is Money

Recent interest in the time-to-execution as a measure of market quality in order driven markets coincides with the emerging empirical research on this topic. We build on recent theoretical models of dynamic limit order book to construct an estimation procedure that tests the effect of the expected time-to-executionen on the order aggressiveness, taking into account the simultaneous determination of the two variables in equilibrium, the selection bias, and the censoring of the time variable. Using very detailed data from the Tel Aviv Stock Exchange, we show that the reduction in the expected time-to-execution is an important determinant of order aggressiveness, and may account for over 50% of the spread. Moreover, we obtain qualitatively similar results for stocks and government bonds that are traded on the same platform, which suggests that immediacy considerations have a significant effect on the high frequency price dynamics regardless of the degree of information asymmetry. We also show that the expected time-to-execution explains the choice of order strategy better than the probability of execution, which was traditionally used in the literature. Finally, our results corroborate predictions of several theoretical models of order driven markets.
Effect of Multisensory Stimulation on Pain of Eye Examination in Preterm Infants

Background Eye examination as one of the painful procedures for retinopathy of prematurity screening can cause some pain-related physiological and behavioral changes in preterm infants. Multisensory stimulation is an analgesic non-pharmacological method that has analgesic effects on infants during painful procedures. Objectives This study aimed to determine the effect of multisensory stimulation on induced pain during eye examination for retinopathy of prematurity screening in preterm infants. Methods In this double-blind clinical trial, 80 preterm infants were randomly divided into two groups. In the intervention group, multisensory stimulation program was performed for 15 minutes before the beginning of examination while the control group received the routine care. Pain score for each infant was recorded by premature infant pain profile. Data were analyzed using independent t-test, Mann-Whitney, and ANOVA with repeated measures by SPSS software (version 16). Results The mean gestational age was 30.4 ± 1.7 weeks in the multisensory stimulation group and 30.6 ± 1.8 weeks in the control group. Based on ANOVA with repeated measures, the pain score was significantly different between two groups during the assessment process (P < 0.001). The changes in pain severity during the examination were also significant between the two groups (P < 0.001); so that the pain was more intensive in the control group than the intervention group. Conclusions Multisensory stimulation program as a safe and easy method can reduce pain in neonates and may be used as a way to reduce pain during eye examination in infants.
Evaluation of Temperature and Stress Distribution on 2 Different Post Systems Using 3-Dimensional Finite Element Analysis

Background The mouth is exposed to thermal irritation from hot and cold food and drinks. Thermal changes in the oral cavity produce expansions and contractions in tooth structures and restorative materials. The aim of this study was to investigate the effect of temperature and stress distribution on 2 different post systems using the 3-dimensional (3D) finite element method. Material/Methods The 3D finite element model shows a labio-lingual cross-sectional view of the endodontically treated upper right central incisor and supporting periodontal ligament with bone structures. Stainless steel and glass fiber post systems with different physical and thermal properties were modelled in the tooth restored with composite core and ceramic crown. We placed 100 N static vertical occlusal loading onto the center of the incisal surface of the tooth. Thermal loads of 0°C and 65°C were applied on the model for 5 s. Temperature and thermal stresses were determined on the labio-lingual section of the model at 6 different points. Results The distribution of stress, including thermal stress values, was calculated using 3D finite element analysis. The stainless steel post system produced more temperature and thermal stresses on the restorative materials, tooth structures, and posts than did the glass fiber reinforced composite posts. Conclusions Thermal changes generated stresses in the restorative materials, tooth, and supporting structures.
Response of growing goslings to dietary supplementation with methionine and betaine

Abstract An experiment with a 2 × 3 factorial design with two concentrations of dietary betaine (0 and 600 mg/kg) and three dietary concentrations of methionine (0, 600 and 1200 mg/kg) was conducted using goslings to estimate growth, nutrient utilisation and digestibility of amino acids from 21 to 70 d of age. Three hundred geese were randomised at 18 d of age into 6 groups with 5 replicates per treatment and 10 geese per replicate. Increasing dietary concentrations of methionine gave a linear increase in body weight and average daily gain. The coefficient of crude fat retention increased as dietary methionine increased and there was a significant non-linear response to increasing dietary methionine. Similarly, increasing supplemental methionine gave linear increases in the digestibility of methionine and cysteine. The results of this study indicated that optimal dietary supplementation of methionine could increase growth performance and methionine and cysteine utilisation in growing goslings. Betaine supplementation had no apparent sparing effect on methionine needs for growth performance, but did improve the apparent cysteine digestibility.
B cell lymphoma, unclassifiable, with features intermediate between diffuse large B cell lymphoma and classical hodgkin lymphoma: Diagnosis by fine‐needle aspiration cytology

A 58‐year‐old lady presented with mediastinal lymphadenopathy. A thoracoscopic ultrasound‐guided fine‐needle aspiration showed large atypical epithelioid cells arranged in cohesive sheets and dispersed as single cells with intact cytoplasm amid a background of lymphocytes and histiocytes. A cytological diagnosis of “a malignant neoplasm” was made, raising a broad list of differential diagnoses. A broad panel of immunocytochemical stains performed on the cell block was indicative of a lymphoproliferative disorder, but the immunophenotype was intermediate between diffuse large B cell lymphoma (DLBCL) and classical Hodgkin lymphoma (cHL). Diffuse and strong reactivity to CD20, CD79a, and PAX‐5, and weak reactivity to CD30, was in favor of a DLBCL, or more precisely mediastinal (thymic) large B cell lymphoma (MLBL). However, there were negative staining for LCA, OCT‐2, and BOB‐1 as well as positive staining for EBV‐encoded RNA, which were against a diagnosis of MLBL and raised the possibility of cHL. The absence of RS cells and the typical mileu, the negativity for CD15 and the strong positivity of CD20 and PAX‐5 were against a diagnosis of cHL. On this basis, the diagnosis of “B‐cell lymphoproliferative disorder with features intermediate between DLBCL and cHL” was rendered. The diagnosis was subsequently confirmed on excisional biopsy. This case report demonstrates broad differential diagnoses raised by this diagnostic entity and the importance of an adequate cell block for accurate designation. Diagn. Cytopathol. 2014;42:690–693. © 2013 Wiley Periodicals, Inc.
Effects of ethanol, molasses and Lactobacillus plantarum on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages in the Tibetan plateau of China.

In Tibet, it is common practice to make and relocate total mixed ration (TMR) silages before feeding due to the uneven distribution of forages temporally and spatially. This study was conducted to investigate the effects of Lactobacillus plantarum (L), molasses (M) or ethanol (E) on the fermentation quality and aerobic stability of local adaptive TMR silage. After 45 days of ensiling, pH and ammonia nitrogen in inoculated TMR silages were significantly lower than control. During the first 6 days of the aerobic exposure test, a small fluctuation in lactic acid concentration for all TMR silages was observed, and then silages with ethanol continued this trend, while lactic acid in silage without ethanol sharply decreased until the end of the aerobic exposure period. Meanwhile, pH gradually increased along the aerobic exposure; silages treated with ethanol showed lower pH after 9 days of aerobic exposure. The population of yeast gradually increased during 6 days of aerobic exposure, after that an accelerated rise was observed in TMR silages without ethanol. The combinational beneficial effect of L. plantarum and ethanol was found in combined addition of ethanol and Lactobacillus plantarum silages (EL), indicated by intermediate fermentation quality and higher aerobic stability.