Miracle-dz/UniFAR-FTUs huggingface dataset:
Dual Primary Gastric and Colorectal Cancer: Is the Prognosis Better for Synchronous or Metachronous?

ObjectivesThe purpose of this study was to investigate the prognosis and clinicopathologic features of synchronous and metachronous dual primary gastric and colorectal cancer (DPGCC). MethodsWe reviewed clinical data of 96 patients with DPGCC, comprising 63 men and 33 women, from among 4523 patients with gastric or colorectal cancer who underwent surgical treatment or chemotherapy, but no endoscopic resection, for colorectal cancer between 1990 and 2009. The selected patients were classified into 2 groups according to the time of gastric or colorectal cancer detection as follows: synchronous group (n= 42) and metachronous group (n = 54). ResultsAmong 4523 patients treated for gastric and colorectal cancer, DPGCC was diagnosed in 96 (2.1%). In terms of clinicopathologic features, the proportion of early-stage gastric and colorectal cancer was higher in the metachronous DPGCC group, with P values of 0.02 and 0.01, respectively. Overall survival in the metachronous DPGCC group was significantly longer than in the synchronous DPGCC group (P = 0.02). Metachronous DPGCC was identified as an independent predictor of survival by both univariate and multivariate analyses, with a P value of 0.02 and 0.006, respectively. ConclusionMetachronous DPGCC had a better prognosis than synchronous DPGCC due to the tendency for metachronous DPGCC to occur in patients with early-stage cancer.
A quantitative approach to modeling mammalian myelinated nerve fibers for electrical prosthesis design

Presents an upgraded cable model of mammalian myelinated nerve fibers in an extracellularly applied field. The kinetics of the nodes is based upon voltage clamp data in rat motor fibers at 37/spl deg/C (J.R. Schwartz and G. Eikhof, 1987), while the resting membrane potential is computed with the Goldman equation. The resulting spike shape, conduction velocity, strength/duration behavior, and absolute and relative refractory period are in good quantitative agreement with published experimental data in mammals at normal body temperature and at 20/spl deg/C. Results at intermediate temperatures however, suggest that the widely used concept of a constant Q/sub 10/ for the rate constants is invalid. In addition, the model generates realistic abortive spikes towards the end of the absolute refractory period and it can describe the consequences of repetitive firing. The results stress the advantages of a multiple nonlinear node model even if only time aspects of nerve behavior are under study. It turned out, that the model presented here describes in vivo neural properties relevant for electrical prosthesis design better than previous models in literature. <<ETX>>
Diversity of capitalism and macroeconomic policy

The analysis of the relations between diversity/variety of capitalism and macroeconomic policy is still largely undeveloped. According to Soskice [2007], discretionary macroeconomic policy would be suited to liberal market economies whereas more orthodox macroeconomics would t with coordinated market economies. Following this conclusion, the dominant macro policy in Europe nowadays would be more favourable to the emergence of a CME than that of a neo-liberal model of capitalism. On the other hand, orthodox macroeconomic policy is often blamed for its neoliberal inspiration. The contribution will try to analyse some of the linksbetween macroeconomic policy and institutional features di¤erentiating models of capitalism.
Pilot expertise and hippocampal size: associations with longitudinal flight simulator performance.

BACKGROUND Previous research suggests that the size of the hippocampus can vary in response to intensive training (e.g., during the acquisition of expert knowledge). However, the role of the hippocampus in maintenance of skilled performance is not well understood. The Stanford/Veterans Affairs Aviation MRI Study offers a unique opportunity to observe the interaction of brain structure and multiple levels of expertise on longitudinal flight simulator performance. METHODS The current study examined the relationship between hippocampal volume and three levels of aviation expertise, defined by pilot proficiency ratings issued by the U.S. Federal Aviation Administration (11). At 3 annual time points, 60 pilots who varied in their level of aviation expertise (ages ranging from 45 to 69 yr) were tested. RESULTS At baseline, higher expertise was associated with better flight simulator performance, but not with hippocampal volume. Longitudinally, there was an Expertise x Hippocampal volume interaction, in the direction that a larger hippocampus was associated with better performance at higher levels of expertise. DISCUSSION These results are consistent with the notion that expertise in a cognitively demanding domain involves the interplay of acquired knowledge ('mental schemas') and basic hippocampal-dependent processes.
Where is the evidence? Realising the value of grey literature for public policy & practice: a discussion paper

The internet has profoundly changed how we produce, use and collect research and information for public policy and practice, with grey literature playing an increasingly important role. The authors argue that grey literature (i.e. material produced and published by organisations without recourse to the commercial or scholarly publishing industry) is a key part of the evidence produced and used for public policy and practice. Through surveys of users, producing organisations and collecting services a detailed picture is provided of the importance and economic value of grey literature. However, finding and accessing policy information is a timeconsuming task made harder by poor production and management of resources and a lack of large-scale collection services able to host and make available relevant, high-quality resources quickly and efficiently. The paper makes recommendations for changes that would maximise the benefits of grey literature in the public interest and seeks feedback from readers to inform the final report of the research project.
Lipid synthesis by rumen microorganisms. II. Further characterization of the effects of methionine.

Abstract Characteristics of the lipids formed by rumen microbes as a result of methionine stimulation were investigated. Tracer studies with acetate, glucose, and long-chain fatty acids revealed substantial transfer of carbon from these sources to complex microbial lipids. The extensive labeling of a variety of lipid compounds, and especially the stimulation of polar lipid formation, suggests that the lipids formed were serving as structural components related to microbial growth. It is believed that most of this new lipid was associated with rumen protozoa.
Calcium Inhibition and Calcium Potentiation of Orai1, Orai2, and Orai3 Calcium Release-activated Calcium Channels*

The recent discoveries of Stim1 and Orai proteins have shed light on the molecular makeup of both the endoplasmic reticulum Ca2+ sensor and the calcium release-activated calcium (CRAC) channel, respectively. In this study, we investigated the regulation of CRAC channel function by extracellular Ca2+ for channels composed primarily of Orai1, Orai2, and Orai3, by co-expressing these proteins together with Stim1, as well as the endogenous channels in HEK293 cells. As reported previously, Orai1 or Orai2 resulted in a substantial increase in CRAC current (Icrac), but Orai3 failed to produce any detectable Ca2+-selective currents. However, sodium currents measured in the Orai3-expressing HEK293 cells were significantly larger in current density than Stim1-expressing cells. Moreover, upon switching to divalent free external solutions, Orai3 currents were considerably more stable than Orai1 or Orai2, indicating that Orai3 channels undergo a lesser degree of depotentiation. Additionally, the difference between depotentiation from Ca2+ and Ba2+ or Mg2+ solutions was significantly less for Orai3 than for Orai1 or -2. Nonetheless, the Na+ currents through Orai1, Orai2, and Orai3, as well as the endogenous store-operated Na+ currents in HEK293 cells, were all inhibited by extracellular Ca2+ with a half-maximal concentration of ∼20 μm. We conclude that Orai1, -2, and -3 channels are similarly inhibited by extracellular Ca2+, indicating similar affinities for Ca2+ within the selectivity filter. Orai3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to the process of Ca2+ depotentiation.
Design of PEG-grafted-PLA nanoparticles as oral permeability enhancer for P-gp substrate drug model Famotidine

Abstract Bioavailability of oral drugs can be limited by an intestinal excretion process mediated by P-glycoprotein (P-gp). Polyethylene glycol (PEG) is a known P-gp inhibitor. Dispersion of Famotidine (a P-gp substrate) within PEGylated nanoparticles (NPs) was used to improve its oral bioavailability. In this work, we evaluated the potential impact of NPs prepared from a grafted copolymer of polylactic acid and PEG on P-gp function by studying in vitro permeability of Famotidine across Caco-2 cells. Copolymers of PEG grafted on polylactic acid (PLA) backbone (PLA-g-PEG) were synthesised with 1 mol% and 5 mol% PEG vs. lactic acid monomer using PEG 750 and 2000 Da. The polymers were used to prepare Famotidine-loaded NPs and tested in vitro on Caco-2 cells. Significant decrease in basolateral-to-apical transport of Famotidine was observed when Famotidine was encapsulated in NPs prepared from PLA-g-PEG5%. NPs prepared from PLA-g-PEG5% are promising to improve oral bioavailability of P-gp substrates.
Microbiologic and Clinical Features of Salmonella Species Isolated From Bacteremic Children in Eastern Democratic Republic of Congo

Background: The morbidity of Salmonella bloodstream infections is unacceptably high in Africa. In 2000, the WHO Global Salmonella-Surveillance (GSS) program was founded to reduce the health burden of foodborne diseases. The incorporation, in 2002, of the Democratic Republic of Congo (DRC) in this program allowed the improvement of laboratory capacities. In this retrospective study, we describe the first signs of impact the GSS program has had in DRC in the management of bacteremia. Methods: Between 2002 and 2006, we evaluated, in one pediatric hospital, the microbiologic and clinical features of Salmonella isolated from children suspected of having bacteremia. A random selection of isolates was typed by pulsed field gel electrophoresis (PFGE). Results: Among the 1528 children included in the study, 26.8% were bacteremic. Salmonella accounted for 59% of all bloodstream infections. Salmonella typhimurium (60.5%) and Salmonella enteritidis (22.3%) were the most common Salmonella serotypes. In total, 92.4% were resistant to at least 3 antimicrobials with the following proportion of strains resistant to: ampicillin (86%), chloramphenicol (92%), trimethoprim/sulfamethoxazole (95%), and tetracycline (34%). In 2002, 32.1% of children received an appropriate empiric antimicrobial treatment. In 2006, with the restoration of the confidence in the results provided by the laboratory, we observed an increase of the proportion of patients appropriately (82.9%) treated with antimicrobials (P < 0.01) without any decrease in the overall mortality rates associated with salmonellae bacteremia. Conclusions: Our findings indicate the benefit to strengthen laboratory capacities in Africa, allowing the development of management guidelines of bloodstream infection.
Analysis of continuous-wave obturation using a single-cone and hybrid technique.

This study analyzed the adaptation of gutta-percha to prepared root canal walls using two obturation techniques and determined the influence of the System-B plugger depth on filling adaptation. Fifty-six extracted human mandibular molars were instrumented using Profile NiTi rotary instruments, stratified based on curvature, then randomly distributed into two groups. Group 1 was obturated using the single-cone continuous-wave technique. Group 2 was obturated with a hybrid technique: lateral condensation followed by a continuous-wave down-pack. Based on System-B plugger penetration, teeth were divided into three subgroups: (a) < 3.5 mm, (b) 3.5 to 4.5 mm, and (c) > 4.5 mm. Roots were horizontally sectioned at 1 mm and 3 mm coronal to the apical foramen, stained, and photographed. Four evaluators scored the adaptation of gutta-percha to the prepared canal walls. In 100% (n = 56) of the samples, no statistically significant difference existed between the two obturation methods at 1-mm (x = 1.80, SD +/- 0.69) or 3-mm (x = 1.804, SD +/- 0.69) sections. Best results were obtained with a plugger depth 3.5 to 4.5 mm from the working length.
Analysis of Active Hybrid Fiber-Semiconductor Devices for Optical Networks

We present an evanescent coupling method between a semiconductor antiresonant reflecting optical waveguide and a side-polished fiber and apply it to the design and fabrication of several hybrid fiber-semiconductor devices. Unique properties and advantages of these devices over conventional fiber-coupled modules are discussed in detail. Previous realizations of this coupling method have all been one-dimensional, as no lateral confinement mechanism was used. In this paper, we have successfully achieved great improvements in efficiency of coupling by implementing lateral confinement as well. In particular, we demonstrate the first side-coupled fiber semiconductor laser based on this technology, coupling more than 3 mW of power at 850 nm directly into a 5/125 mum single-mode fiber.
Physiology of the Heart

In the single authored, expert voice that is this book's unique strength, Arnold Katz provides a comprehensive overview of the physiological and biophysical basis of cardiac function, beginning with structure and proceeding to biochemistry, biophysics, and pathophysiology in arrhythmias, ischemia, and heart failure. Emphasis is on the interrelationships of basic processes among the cell, cardiac muscle function, and the biophysics of contractile and electrical behavior. This edition includes new material on cell signaling and molecular biology.
A Self-Excisable Infectious Bacterial Artificial Chromosome Clone of Varicella-Zoster Virus Allows Analysis of the Essential Tegument Protein Encoded by ORF9

ABSTRACT In order to facilitate the generation of mutant viruses of varicella-zoster virus (VZV), the agent causing varicella (chicken pox) and herpes zoster (shingles), we generated a full-length infectious bacterial artificial chromosome (BAC) clone of the P-Oka strain. First, mini-F sequences were inserted into a preexisting VZV cosmid, and the SuperCos replicon was removed. Subsequently, mini-F-containing recombinant virus was generated from overlapping cosmid clones, and full-length VZV DNA recovered from the recombinant virus was established in Escherichia coli as an infectious BAC. An inverted duplication of VZV genomic sequences within the mini-F replicon resulted in markerless excision of vector sequences upon virus reconstitution in eukaryotic cells. Using the novel tool, the role in VZV replication of the major tegument protein encoded by ORF9 was investigated. A markerless point mutation introduced in the start codon by two-step en passant Red mutagenesis abrogated ORF9 expression and resulted in a dramatic growth defect that was not observed in a revertant virus. The essential nature of ORF9 for VZV replication was ultimately confirmed by restoration of the growth of the ORF9-deficient mutant virus using trans-complementation via baculovirus-mediated gene transfer.
When Does Leverage Hurt Performance? - A Firm-Level Analysis

In the wake of the global financial crisis, several macroeconomic contributions have highlighted the risks of excessive credit expansion. In particular, too much finance can have a negative impact on growth. We examine the microeconomic foundations of this argument, positing a non-monotonic relationship between leverage and firm-level productivity growth in the spirit of the trade-off theory of capital structure. A threshold regression model estimated on a sample of Central and Eastern European countries confirms that TFP growth increases with leverage until the latter reaches a critical threshold beyond which leverage lowers TFP growth. This estimate can provide guidance to firms and policy makers on identifying “excessive” leverage. We find a similar non-monotonic relationship between leverage and proxies for firm value. Our results are a first step in bridging the gap between the literature on optimal capital structure and the wider macro literature on the finance-growth nexus.
An iris recognition based on dual tree complex wavelet transform

With an increasing emphasis on security, automated personal identification and verification based biometrics has been receiving extensive attention over the past decade. The iris images captured from the same person or different persons differ in size due to illumination variations and other factors. The iris recognition system is composed of many steps. Namely, capturing the iris patterns; determining the location of the iris boundaries; converting the iris boundary to the polar coordinate system; extracting the iris code based on wavelet transforms; and classification of the iris code. There are many methods proposed for iris recognition by many researchers. The proposed system based on dual tree complex wavelet transform for iris recognition system with high performance and acceptance/rejection capabilities is described.
Immunization with Trypanosoma cruzi epimastigote antigens incorporated into iscoms protects against lethal challenge in mice

An immunoglobulin G3 monoclonal antibody obtained by immunizing mice with a cell membrane preparation of epimastigotes of Trypanosoma cruzi was shown to agglutinate live epimastigotes, lyse blood-form trypanosomes, and partially protect mice by passive transfer. The main antigens recognized by the monoclonal antibody were located in the flagella of epimastigotes and blood-form trypanosomes. Antigens of epimastigotes, purified by affinity chromatography with the monoclonal antibody, were shown to be highly glycosylated and revealed a wide band with an Mr between 45,000 and 68,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. Immunization of mice with a small concentration of the affinity purified antigens incorporated into an antigen delivery system prepared with Quil A (Isotec AB, Lulea, Sweden), a saponin derivative, induced strong antibody and cell-mediated immune responses and protected 100% of the immunized animals against death due to challenge with 100 100% lethal doses of blood form trypanosomes. Protection was due to the use of the antigen delivery system, since mice immunized with equal concentrations of antigens alone or in combination with saponin had 100% mortality. The results suggest that small concentrations of epimastigote antigens obtained by biochemical methods and incorporated into the proper antigen delivery system may serve as a vaccine against Chagas' disease.
Active Participation of Antigen-Nonspecific Lymphoid Cells in Immune-Mediated Inflammation1

The pathogenic process of tissue-specific autoimmune disease depends to a large extent on recruitment of Ag-nonspecific cells into the target tissue. Little is known, however, about the recruitment process and the features that characterize the recruited cells. In this study, we analyzed the recruitment of Ag-nonspecific lymphoid cells into an inflammatory site by using an experimental system in which TCR-transgenic Th1 cells are adoptively transferred to induce ocular inflammation in recipient mice that express the target Ag in their eyes. A sharp increase in number of all host cell populations was observed in the recipient spleen, reaching a peak on day 4 postcell transfer and declining thereafter. A large portion of the host’s spleen CD4 cells underwent phenotypic changes that facilitate their migration into the target organ, the eye. These changes included increased expression of the chemokine receptor CXCR3, and the adhesion molecule CD49d, as well as a decline in expression of CD62L. The host lymphocytes migrated into the recipient mouse eye more slowly than the donor cells, but became the great majority of the infiltrating cells at the peak of inflammation on day 7 postcell injection. Interestingly, the mass migration of host T cells was preceded by an influx of host dendritic cells, that reached their peak on day 4 postcell injection. The eye-infiltrating host CD4 lymphocytes underwent additional changes, acquiring a profile of activated lymphocytes, i.e., up-regulation of CD25 and CD69. Our results thus provide new information about the active participation of Ag-nonspecific lymphoid cells in immune-mediated inflammation.
The Employment Equity Act, 1998 (And Other Myths About the Pursuit of 'Equality', 'Equity' and 'Dignity' in Post-Apartheid South Africa) Part 1

The author critically examines the organising principle of the affirmative action provisions of the Employment Equity Act (or EEA), as well as the implications of the recent judgment by the Constitutional Court in its first case involving the application of affirmative action in the employment context (and in terms of the EEA) – SAPS v Solidarity obo Barnard. While reiterating the need for restitutionary measures such as affirmative action in South Africa, the author concludes – probably quite controversially - that the EEA's treatment of affirmative action has nothing to do with the equality right in the Bill of Rights, and that the Act pursues a different (and omnipresent) social engineering agenda by the state. The author calls for this realisation to prompt future affirmative action cases arising from the application of this Act to be removed from the scheme of (and potential defences available under) the equality jurisprudence, and for the courts to critically interrogate the constitutionality of the EEA's affirmative action scheme within its own context. The author believes that Chapter III of the Act is unconstitutional in this sense, and he calls for the scrapping of its provisions. He also calls for a (more) constitutionallycompliant exposition from the Constitutional Court of the parameters of legitimate affirmative action under the Bill of Rights, and adds his voice to the numerous calls for reconsideration of the "rationality test" expounded in Minister of Finance v van Heerden. More generally, the author considers the apparently all-pervasive application of the government ideology of the pursuit of demographic representivity in "transformation" of employment and other contexts (expressing grave doubts about its constitutionality along the way). This article forms Part 1 of this piece and the author considers the constitutional requirements for a legitimate affirmative action programme or measure. He then examines the affirmative action scheme of the Employment Equity Act, and explains his views on why such scheme is, in fact, unconstitutional. In Part 2 of this piece (which follows in this edition), the author continues to critically evaluate the Constitutional Court judgment in the Barnard case, and he highlights the biggest areas of disappointment of this judgment within the context of South Africa's equality jurisprudence. After a very brief consideration of the recent amendments to the Employment Equity Act, the author concludes by providing reasons why the Act's approach to affirmative action needs to be rejected, and soon.