Miracle-dz/UniFAR-FTUs huggingface dataset:
Acupuncture in the Treatment of Painful Dysfunction of the Temporomandibular Joint – a Review of the Literature

Objective: The aim of the present study was to analyze the results of randomized clinical trials on the efficacy of acupuncture in the treatment of painful dysfunction of the temporomandibular joint. Materials and Methods: A literature search of 3 electronic databases was performed, and only randomized studies comparing acupuncture-treated patients with either untreated or conservatively treated control groups were included. For this purpose, results were compared in narrative and tabular form. Results: To date, only 8 publications representing 6 randomized clinical trials have looked into the efficacy of acupuncture in the treatment of craniomandibular dysfunction. With the exception of one, all studies were published in Sweden, between 1985 and 1992. A more recent US study was the only one to apply sham acupuncture to test the efficacy of acupuncture. All studies share methodological shortcomings, including a lack of detailed descriptions of the randomization procedures, point selection, possible dropouts and undesirable events as well as attempts to identify a possible placebo effect of the acupuncture. Only 1 study investigated long-term results. Although based on the improvement of subjective and objective criteria, all studies consider acupuncture as an effective treatment for painful dysfunction of the temporomandibular joint, the good results achieved must be interpreted with caution because of the methodological shortcomings identified. Conclusion: Acupuncture appears to be a suitable complementary treatment method in the management of craniomandibular dysfunction. However, its significance has to be further evaluated in future studies.
Colorectal cancer liver metastases - understanding the differences in the management of synchronous and metachronous disease.

INTRODUCTION Metastatic disease to the liver in colorectal cancer is a common entity that may present synchronously or metachronously. While increasing surgical experience has improved survival outcomes, some evidence suggest that synchronous lesions should be managed differently. This review aims to update current literature on differences between the outcomes and management of synchronous and metachronous disease. MATERIALS AND METHODS Systematic review of MEDLINE database up till November 2008. RESULTS Discrete differences in tumour biology have been identified in separate studies. Twenty-one articles comparing outcomes were reviewed. Definitions of metachronicity varied from anytime after primary tumour evaluation to 1 year after surgery for primary tumour. Most studies reported that synchronous lesions were associated with poorer survival rates (8% to 16% reduction over 5 years). Sixteen articles comparing combined vs staged resections for synchronous tumour showed comparable morbidity and mortality. Benefits over staged resections included shorter hospital stays and earlier initiation of chemotherapy. Suitability for combined resection depended on patient age and constitution, primary tumour characteristics, size and the number of liver metastases, and the extent of liver involvement. CONCLUSIONS Surgery remains the only treatment option that offers a chance of long-term survival for patients amenable to curative resection. Synchronicity suggests more aggressive disease although a unifying theory for biological differences explaining the disparity in tumour behaviour has not been found. Combined resection of primary tumour and synchronous metastases is a viable option pending careful patient selection and institutional experience. Given the current evidence, management of synchronous and metachronous colorectal liver metastases needs to be individualised to the needs of each patient.
Overexpression of bax induces down‐regulation of store‐operated calcium entry in prostate cancer cells

Store‐operated Ca2+ channels control homeostasis between extracellular Ca2+ reservoir and intracellular Ca2+ storage and play important roles in apoptosis in a wide variety of cells, including prostate epithelia. Recent studies have shown that the acquired apoptosis‐resistant nature of androgen‐independent prostate cancer is associated with reduced function of store‐operated Ca2+ entry (SOCE). This study investigates the functional interaction between Bax and SOCE in the apoptosis signaling cascade in prostate cancer. Our previous findings show that NRP‐154, an androgen‐independent prostate cancer cell line, could sustain overexpression of exogenous Bax without undergoing apoptosis. Here we show that sustained overexpression of Bax in NRP‐154 cells leads to down‐regulation of SOCE and reduced Ca2+ storage inside the endoplasmic reticulum. While reduced SOCE may represent an adaptive mechanism for cell survival, increased levels of Bax in the latent state enhances the sensitivity of NRP‐154 cells to TGF‐β and thapsigargin‐induced apoptosis. This enhanced apoptosis can be reduced by 2‐aminoethoxydiphenyl borate (2‐APB), an inhibitor of SOCE, or reversed under conditions where SOCE is only partially activated. Our results demonstrate a functional interaction between Bax and SOCE in apoptosis of prostate cancer, and support the concept that improving this interaction has therapeutic implications for prostate cancer. J. Cell. Physiol. 216: 172–179, 2008. © 2008 Wiley‐Liss, Inc.
Speech Recognition in Noise by Younger and Older Adults

Objectives: Purpose of the present study is to evaluate the effects of aging on temporal resolution and speech understanding in noise. Methods: Twenty-seven young and 33 older normal hearing adults participated in this study. Temporal resolution was investigated using the Gaps in Noise (GIN) test. Understanding sentences in noise was investigated using a Dutch sentences (LIST) test. Results: Results demonstrated that older adults have significantly worse temporal resolution scores and significantly greater difficulty understanding sentences in noise than younger adults. In the group of older adults, the ability to understand sentences in noise correlated with hearing and age. A stepwise regression analysis showed that age was the best predictor for the speech in noise results. However, temporal resolution correlated with hearing, not with age. Results from the GIN test did not correlate with the results from the LIST test. Conclusions: The present study shows that with advancing age, both temporal resolution and speech understanding in noise significantly diminish. Temporal resolution correlates with hearing, not age, while speech understanding correlates more with age than hearing. These results could have implications for the outcome of auditory prostheses in older adults, as both diminished temporal resolution and speech understanding have to be taken into account.
X‐ray kinematic analysis of shoulder movements during target reaching and food taking in the cat

Co‐ordinate movements around the shoulder are essential during reaching movements. We performed a quantitative kinematic analysis of movements of the shoulder girdle: three‐dimensional X‐ray frames (time resolution 20 ms) were recorded during the target‐reaching and food‐taking paradigm in five cats either sitting (n = 4) or standing (n = 1) in front of a food well. Movements of the scapula consisted of a flexion of the scapula (anteversion of the glenoid) followed by flexion of the gleno‐humeral joint (decrease in the angle between the scapular spine and humerus). In the sitting animals, the gleno‐humeral flexion reversed to extension some 120 ms before object contact, while in the standing animal flexion continued during the ongoing scapular flexion. In both cases, the scapula was nearly horizontal at the end of target reaching. The fulcrum for scapular movements was located near the vertebral border of the scapula at the medial elongation of the scapular spine. No major translational components of the fulcrum with respect to the trunk were found during reaching. Together with full flexion of the scapula, this reduces the number of degrees of freedom considerably and thereby probably simplifying the specification of the end‐point of the limb chain. End‐point specification is further supported by rotational movements of the scapula. In the sitting animal, the amplitude of inward rotation along the long axis of the scapula was around 20 °, while it was much more variable in the standing animal, reflecting more variable starting positions. We hypothesize that the glenoid is used to ‘foveate’ the target object.
Comparison of the Levels of Bacterial Diversity in Freshwater, Intertidal Wetland, and Marine Sediments by Using Millions of Illumina Tags

ABSTRACT Sediment, a special realm in aquatic environments, has high microbial diversity. While there are numerous reports about the microbial community in marine sediment, freshwater and intertidal sediment communities have been overlooked. The present study determined millions of Illumina reads for a comparison of bacterial communities in freshwater, intertidal wetland, and marine sediments along Pearl River, China, using a technically consistent approach. Our results show that both taxon richness and evenness were the highest in freshwater sediment, medium in intertidal sediment, and lowest in marine sediment. The high number of sequences allowed the determination of a wide variety of bacterial lineages in all sediments for reliable statistical analyses. Principal component analysis showed that the three types of communities could be well separated from phylum to operational taxonomy unit (OTU) levels, and the OTUs from abundant to rare showed satisfactory resolutions. Statistical analysis (LEfSe) demonstrated that the freshwater sediment was enriched with Acidobacteria, Nitrospira, Verrucomicrobia, Alphaproteobacteria, and Betaproteobacteria. The intertidal sediment had a unique community with diverse primary producers (such as Chloroflexi, Bacillariophyta, Gammaproteobacteria, and Epsilonproteobacteria) as well as saprophytic microbes (such as Actinomycetales, Bacteroidetes, and Firmicutes). The marine sediment had a higher abundance of Gammaproteobacteria and Deltaproteobacteria, which were mainly involved in sulfate reduction in anaerobic conditions. These results are helpful for a systematic understanding of bacterial communities in natural sediment environments.
Cumulative conception and live birth rates after the treatment of anovulatory infertility: safety and efficacy of ovulation induction in 200 patients.

An analysis was performed on the cumulative conception rates, cumulative live birth rates and adverse effects of ovulation induction in patients with anovulatory infertility attending a single unit over an 11-year period. A total of 200 patients were included, 103 with clomiphene-resistant polycystic ovary syndrome (PCOS), 77 with hypogonadotrophic hypogonadism (HH) and 20 with weight-related amenorrhoea (WRA). Ovulation induction was performed using a number of protocols in which pulsatile luteinizing hormone-releasing hormone was administered s.c. or i.v. and gonadotrophins (human menopausal gonadotrophins or follicle-stimulating hormone) were administered i.m. The cumulative conception and live birth rates in the first course of therapy and after 12 cycles of treatment were, respectively, 73.2 and 62.4% in PCOS patients, 82.1 and 65.4% in the HH group and 95.0 and 85.3% in the WRA group. The miscarriage rates for all courses of treatment were 15.5% in PCOS patients, 22.9% in HH patients and 32.3% in WRA patients which resulted in cumulative live birth rates that were not significantly different. The median number of cycles and ovulations to achieve a pregnancy was 2 in all groups. The multiple pregnancy rate was significantly greater in women with PCOS (17.9%) than in women with HH (3.6%, P = 0.0052, 95% CI 5.12-23.36%) but not WRA (3.2%, P = 0.07, 95% CI 4.35-24.92%). The rate of multiple pregnancy fell after the introduction of monitoring by transvaginal ultrasound. Correction of anovulatory infertility by appropriately selected ovulation induction regimens results in cumulative conception and live birth rates indistinguishable from normal.
Müller cell swelling, glutamate uptake, and excitotoxic neurodegeneration in the isolated rat retina

We characterized morphological effects of the endogenous excitotoxin, glutamate in ex vivo retinal segments prepared from 30‐day‐old rats. Initial changes induced by glutamate consisted of reversible, sodium‐dependent Müller cell swelling. This glial swelling was mimicked by glutamate transport substrates but not by ionotropic glutamate receptor agonists. Only very high concentrations of exogenous glutamate (3,000 μM) produced excitotoxic neuronal damage. The neuronal damage was accompanied by severe glial swelling and was blocked by an antagonist of non‐N‐methyl‐D‐aspartate (NMDA) receptors but not by an NMDA receptor antagonist. Because glutamate uptake can be influenced by changes in cellular energy levels, we studied the effects of oxidative and glycolytic energy depletion on glutamate‐mediated Müller cell swelling. Oxygen deprivation produced little morphological change and did not alter either glutamate‐mediated Müller cell swelling or glutamate‐induced excitotoxicity. In contrast, inhibition of glycolysis by iodoacetate produced severe neuronal damage without Müller cell swelling. In the presence of iodoacetate, exogenous glutamate failed to cause glial swelling. The neuronal damage produced by iodoacetate was inhibited by pyruvate, a substrate that sustains oxidative energy pathways. In the presence of iodoacetate plus pyruvate, glutamate failed to cause Müller cell swelling but became neurotoxic at low concentrations through activation of non‐NMDA receptors. These results indicate that glycolytic energy metabolism plays a critical role in sustaining ionic balances required for Müller cell glutamate uptake and glial uptake helps to prevent glutamate‐mediated excitotoxicity. GLIA 25:379–389, 1999. © 1999 Wiley‐Liss, Inc.
Potential antioxidant activity of the moss Bryum moravicum

A limited number of mosses has ever been studied regarding antioxidant activity among which only a few are European species. As a part of our ongoing screening of new promising bryophyte resources of natural antioxidants, a relevant biological activity investigation was conducted on the moss Bryum moravicum Podp. (Bryaceae) collected in Germany. The antioxidant activity of an aqueous extract of the species was evaluated in vitro by analysing its ABTS [2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)] cation scavenging activity and total phenolic content. The study led to the detection of a moderate antioxidant activity: 1 mg of the extract was equivalent to 84.56 ± 7.93 µg of ascorbic acid. The total phenolic content in 1 mg of the extract was equivalent to 356.44 ± 9.56 µg of ferulic acid used as a standard. These results have indicated for the first time potential importance of B. moravicum in searching for novel antioxidant agents.
Infusion phlebitis in patients with acute pneumonia: a prospective study.

STUDY OBJECTIVES To prospectively assess the relative risk for phlebitis in a series of consecutive patients with pneumonia and to identify risk factors that predict an increased risk for phlebitis. SETTING Internal medicine department of a tertiary teaching hospital. PATIENTS Seven hundred sixty-six consecutive patients with acute pneumonia receiving IV therapy. INTERVENTIONS Only the first catheter was taken into account. There were 308 short lines (a 51-mm, 18-gauge Teflon catheter); 307 midsized lines (a 28-cm, 16-gauge polyvinyl chloride catheter); and 151 long lines (71-cm, 14-gauge plain polyurethane catheter). Eighteen variables were prospectively evaluated in an open, nonrandomized study for their contribution to the occurrence of phlebitis. RESULTS The overall phlebitis rate was 39%. Phlebitis developed in 53% of patients with short lines, in 41% of patients with midsized lines, and in 10% of patients with long lines, and these catheters remained in place an average (+/- SD) of 3.0+/-2.4 days, 4.6+/-3.4 days, and 7.8+/-6.6 days, respectively. The variables that influenced the development of phlebitis, as determined by multivariate analysis, were the following: type of catheter; blood hemoglobin levels; and IV therapy with either corticosteroids or erythromycin. CONCLUSIONS According to our data, when the use of a catheter is expected to be required for < or = 36 h, a short line can be used. If a longer duration is expected, a longer line is warranted. Ours is the first study in which the relationship between blood hemoglobin levels and phlebitis has been reported. Because the use of intravascular devices is increasingly common, a more complete knowledge of the factors that influence their acceptance has become essential.
Multiple components of voltage-dependent potassium current in normal rat anterior pituitary cells.

1. Voltage-dependent K+ currents were studied in normal rat anterior pituitary cells using the patch-clamp technique. To obtain cultures enriched for lactotrophs, density gradient centrifugation was performed on pituitary cells isolated from lactating rats. 2. Depolarizations to about -30 mV from a holding potential of -80 mV activate a rapidly inactivating [time constant (tau) approximately 15-20 ms at -20 mV]K+ current. This transient current activated at low voltages (termed IA) is abolished by 5 mM external 4-aminopyridine (4-AP) but is largely resistant to external tetraethylammonium (TEA) (< or = 30 mM). 3. Recovery from inactivation of IA is fast, with a tau of 100-200 ms at -80 mV. Deactivation is also fast (tau approximately 2.2 ms at -50 mV). The voltage of half-activation of IA is approximately -20 mV. The current is completely inactivated at a holding potential of -40 mV. 4. Voltage-dependent K+ current activated by depolarizations from a holding potential of -40 mV was first detectable at about -20 mV (high voltage-activated) and had a time course that varied among cells. 5. Deactivation of high voltage-activated K+ current was best described by the sum of two exponentials, with tau of about 3.7 and 30 ms at -50 mV. Both components reversed close to the equilibrium potential for K+. 6. The amplitudes of the two tail currents were independent of each other when variable-duration commands were used to activate current. The amplitude of the fast component was largest with 10- to 20-ms commands to +40 mV and was reduced (< or = 50%) with 136-ms commands. The slow component amplitude reached a peak by 40 ms and remained constant for commands < or = 136 ms at +40 mV. 7. The contribution of each component to the total high voltage-activated tail current was variable among cells, with the amount of fast component correlating with the amount of inactivation produced by commands to +40 mV. 8. The two components of tail current activated by depolarizations from the -40 mV holding potential were abolished by external TEA (10 mM). 4-AP (5 mM externally) selectively abolished the fast component of high voltage-activated tail current while only partially reducing the slow component. 9. These results suggest that normal rat anterior pituitary cells possess at least three distinct types of voltage-dependent K+ current: a low voltage-activated, transient current (IA) and two high voltage-activated currents. (ABSTRACT TRUNCATED AT 400 WORDS)
Anti‐CCR7 monoclonal antibodies as a novel tool for the treatment of chronic lymphocyte leukemia

To date, chronic lymphocytic leukemia (CLL) remains incurable with current treatments, which include the monoclonal antibodies (mAbs) rituximab and alemtuzumab. The efficacy of rituximab is modest when used as single agent, and alemtuzumab induces severe immunosuppression. To develop more potent and specific therapies, we propose the CC chemokine receptor 7 (CCR7) as an attractive target molecule to treat CLL, as it not only fulfills the requirements of a high‐surface expression and a good level of tissue specificity, but it also plays a crucial role in mediating the migration of the tumor cells to lymph nodes (LNs) and thus, in the development of clinical lymphadenopathy. In the current work, murine anti‐human CCR7 mAb mediated a potent, complement‐dependent cytotoxicity (CDC) against CLL cells while sparing normal T lymphocytes from the same patients. The sensitivity to CDC was related to the antigenic density of CCR7. Moreover, these mAb blocked the in vitro migration of CLL cells in response to CC chemokine ligand 19 (CC219), one of the physiological ligands of CCR7. Conversely, CLL cells were poorly lysed through antibody‐dependent, cell‐mediated cytotoxicity (ADCC), probably as a result of the murine origin and the isotype of the anti‐CCR7 mAb used. Molecular engineering techniques will allow us to obtain chimeric or humanized anti‐CCR7 mAb to reach the best clinical response for this common and yet incurable leukemia.
Enhancement of the thermal properties of a vertically aligned carbon nanotube thermal interface material using a tailored polymer

This paper presents our results on thermal properties measurements of thermal interface materials based on vertically aligned carbon nanotubes. In this system, the total interface resistance is the sum of thermal resistances created by the contact between the growth surface and the CNTs, the intrinsic resistance of the CNTs array, and the contact between the loose end of the CNTs and the opposite substrate. The latter is reported to be the limiting factor in the optimization of the global interface resistance, and in this work, polymers are used to enhance the thermal contact. Two polymers are studied. It is shown that the use of a polymer as interface material, despite its low thermal conductivity, leads to an improvement of the total thermal resistance. Experimental data and equilibrium molecular dynamics (EMD) show that the thermal contact resistance when using a reactive polymer - able to establish covalent bonds with the CNTs - is at least three times lower than that with the Van der Waals interaction polymer. The reactive polymer also enhances adhesion between CNTs and the polymer, which allows the mechanical complete transfer of the aligned CNTs array from growth substrate to a copper substrate. This transfer process allows to expand applications for VACNTs.
Insulin sensitivity in type 2 diabetes is closely associated with LDL particle size.

PRINCIPLES Small dense LDLs have been found to be associated with type 2 diabetes (T2DM). This association has been observed in the context of decreased HDL cholesterol and increased triglycerides. METHODS In the present study the relationship between LDL particle size and insulin sensitivity was assessed in 46 patients with T2DM (mean age 60 (11) years, HbA1c 7.6 (0.8) %). 75 g oral glucose tolerance testing was performed and composite insulin sensitivity index was calculated by the method of Matsuda and de Fronzo (ISI(comp)). Additional parameters included BMI, waist circumference, blood pressure, HDL cholesterol, triglycerides and LDL cholesterol. LDL particle size was measured by gradient gel electrophoresis. RESULTS Log ISI(comp) correlated most strongly with LDL particle size (R = 0.61), less closely with HDL cholesterol (R = 0.48) and plasma triglycerides (R = -0.45) and not at all with LDL cholesterol (R = 0.001), as supported by multiple regression analyses where log ISI(com) was associated with LDL particle size (p = 0.004) and HDL cholesterol (p = 0.027) but not with triglycerides and LDL cholesterol. CONCLUSION Log ISI(comp) estimated by a formula using endogenous insulin levels is very closely associated with LDL particle size in patients with T2DM. Our data suggest that smaller LDL particle size reflects the impact of insulin resistance on lipoprotein metabolism more strictly than do the traditional lipid parameters.