Miracle-dz/UniFAR-FTUs huggingface dataset:
Distinct modes of transcription read through or terminate at the c‐myc attenuator.

Premature termination of transcription by RNA polymerase II (pol II) occurs in the 5′ region of many viral and cellular genes. Modulation of this process, or attenuation, is an important means of transcriptional control, but its mechanism is unknown. Using injected Xenopus oocytes, the efficiency of the mouse c‐myc attenuator was tested when it was placed at various distances from the transcription initiation site. The attenuator functioned with each of six different pol II promoters tested; however, termination efficiency declined markedly when it was placed more than approximately 400 bases from the start site. This decline in attenuator function with distance from the start site coincided with increased sensitivity to the pol II inhibitor 5,6‐dichloro‐1‐beta‐D‐ribofuranosyl benzimidazole (DRB). Thus transcription complexes situated further from the promoter appear to have a lower ability to recognize the attenuator and a greater sensitivity to DRB. Furthermore, polymerases which have read through one attenuation site have a reduced ability to terminate at a second site. The results imply that a discrete subset of elongation complexes is capable of premature termination, and that this subset exists only within the first few hundred bases of the transcription unit. Regulation of termination efficiency may be effected by changing the balance between the two modes of transcription committed either to read through or to terminate prematurely.
Long-term follow-up of patients with low-grade malignant lymphomas treated with doxorubicin-based chemotherapy or chemoimmunotherapy.

PURPOSE We reviewed survival data of patients with low-grade lymphoma entered on Southwest Oncology Group (SWOG) lymphoma trials in 1972 to 1983 to determine the utility of doxorubicin-containing therapy (cyclophosphamide, doxorubicin, vincristine, and prednisone [CHOP]) in such patients. PATIENTS AND METHODS We identified all patients with low-grade lymphoma, no prior therapy, and stage III or IV disease who were treated with full-dose CHOP induction therapy on any arm of SWOG studies 7204, 7426, or 7713. Survival data for this group of patients were correlated with pretreatment prognostic factors, including histology, patient age, sex, symptom status, performance status, bone marrow or extranodal involvement, and the number of disease sites. The effect of maintenance treatment was also assessed. RESULTS Four hundred fifteen patients met criteria for inclusion in the study group. With median follow-up periods of 12.8 years (maximum, 19.8 years), the median survival duration was 6.9 years. Survival was significantly shorter in patients with follicular mixed or small lymphocytic histology, age greater than 40 years, male sex, B-symptom status, and SWOG performance status greater than 1. Multivariate regression analysis showed histology, age, and sex to be independent predictors of survival. There was no definite survival plateau of cured patients in any subgroup, although the survival curve for follicular mixed histology patients showed long-term survival of approximately 25%. Maintenance therapy did not prolong survival. CONCLUSION Doxorubicin-containing treatment did not prolong the overall median survival of low-grade lymphoma patients compared with results with less-aggressive programs.
Understanding Information Technology Investment Decision-Making in the Context of Hotel Global Distribution Systems: a Multiple-Case Study

This study investigates what three large, multinational hospitality companies do in practice when evaluating and making IT investment decisions. This study was launched in an attempt to 1) learn more about how multinational hospitality companies evaluate, prioritize, and select IT investments in the context of hotel GDS; 2) call attention to an important and costly topic in hopes of improving current practices; and 3) fill a noticeable literary void so that future researchers on IT and hotel GDS would have a foundation and starting point. The perennial question of any business is “How does an organization add value? ” Value can be defined from many different perspectives and may result from tangible and intangible factors. Principal stakeholders include shareholders (investors), customers, and employees. Shareholders typically measure value in terms of economic return on their investment based upon some level of perceived risk. For customers, value is assessed in terms of a price-value relationship; that is, how much they received in terms of product and services for the price they paid. For employees, value is measured by salary and by the intrinsic rewards of the job. Yet, one of the most elusive questions with respect to information technology is “How can value be measured? ” Hospitality executives are being pressured daily to invest more in information technology (IT)— especially in the area of hotel global distribution systems (GDS), which have become the cornerstone of a hotel firm’s IT infrastructure and portfolio. There are a number of sweeping changes on the horizon impacting hotel GDSs and requiring the development of a well-crafted strategy for global distribution systems. These broad changes include bypass theories to remove airline GDSs and travel agents, the introduction of new and emerging player, and innovative approaches to pricing and promotion. Many of these developments offer promise to hoteliers, but they also threaten their control over their customer relationships and their inventory and add to the complexity and cost of distribution. Selecting the appropriate distribution channels is paramount to success and important if hotel firms are to grow top-line revenue and control overhead; yet the number of choices facing hotel executives is overwhelming. They are also at a loss for measuring value derived from IT.
30.1 8b Thin-film microprocessor using a hybrid oxide-organic complementary technology with inkjet-printed P2ROM memory

We present an 8b general-purpose microprocessor realized in a hybrid oxide-organic complementary thin-film technology. The n-type transistors are based on a solution-processed n-type metal-oxide semiconductor, and the p-type transistors use an organic semiconductor. As compared to previous work utilizing unipolar logic gates [1], the higher mobility n-type semiconductor and the use of complementary logic allow for a >50x speed improvement. It also adds robustness to the design, which allowed for a more complex and complete standard cell library. The microprocessor consists of two parts, a processor core chip and an instruction generator. The instructions are stored in a Write-Once-Read-Many (WORM) memory formatted by a post-fabrication inkjet printing step, called Print-Programmable Read-Only Memory (P2ROM). The entire processing was performed at temperatures compatible with plastic foil substrates, i.e., at or below 250°C [2].
Granulocyte-macrophage colony-stimulating factor after initial chemotherapy for elderly patients with primary acute myelogenous leukemia. Cancer and Leukemia Group B.

BACKGROUND Elderly patients with primary acute myelogenous leukemia (AML) are less likely to enter remission than younger adults, in part because of a higher mortality rate related to severe myelosuppression. Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been shown to shorten the duration of neutropenia and decrease infectious complications when administered after chemotherapy to patients with lymphomas and solid tumors. METHODS We randomly assigned 388 patients 60 years of age or older who had newly diagnosed primary AML to receive placebo or GM-CSF (5 micrograms per kilogram of body weight per day intravenously over a period of six hours) in a double-blind manner, beginning the day after the completion of three days of daunorubicin (45 mg per square meter of body-surface area per day) and seven days of cytarabine (200 mg per square meter per day by continuous intravenous infusion). If leukemia cells persisted in the marrow three weeks after the initiation of chemotherapy, further daunorubicin (two days) and cytarabine (five days) were administered. GM-CSF or placebo was given daily until the neutrophil count was at least 1000 per cubic millimeter, there was evidence of the regrowth of leukemia, or severe toxic effects attributable to the study infusion occurred. Patients who had a complete remission were then randomly assigned to receive one of two intensification regimens. RESULTS Of 388 patients (median age, 69 years), 193 were randomly assigned to receive GM-CSF and 195 to placebo. The rate of complete remission was 51 percent (95 percent confidence interval, 44 to 59 percent) among those assigned to GM-CSF and 54 percent (95 percent confidence interval, 47 to 61 percent) among those assigned to receive placebo (P = 0.61). The reasons for failure (early death, death during marrow hypoplasia, and persistent leukemia), the incidence of severe or lethal infection, and the incidence of the regrowth of leukemia (2 percent overall) were similar in the two groups. The median duration of neutropenia was slightly shorter (P = 0.02) in the patients who received GM-CSF (15 days) than in those who received placebo (17 days), but the clinical importance of this result was minimal because the growth factor failed to lower the treatment-related mortality rate or improve the rate of complete remission. CONCLUSIONS GM-CSF, in the dose and schedule we used, does not stimulate the regrowth of leukemia, but it also does not decrease the severe myelosuppressive consequences of initial chemotherapy or improve the response rate in patients 60 years of age or older with primary AML. It should not be recommended for use in such patients.
TISSUE CARBON DIOXIDE STORES: MAGNITUDE OF ACUTE CHANGE IN THE DOG.

Carbon dioxide washout curves were determined in hyperventilated dogs. Direct measurement of mixed venous carbon dioxide tension allowed calculation of changes in whole-body CO2 stores. The average whole-body CO2 dissociation constant in ten studies was 3.73 ml/kg mm. The limiting factor in reaching a new steady-state value was represented by a slow compartment in the washout curve. The average rate constant for this compartment was 0.062 min–1. The slowest compartment in this analysis has a 98% change in 1 hr, therefore the experimentally determined whole-body dissociation constant should closely approximate actual changes in tissue CO2 stores, excluding bone and fat.
[Trends in coronary artery bypass surgery: changes in the profile of the surgical patient].

INTRODUCTION AND OBJECTIVES In the last few years, the percentage of high-risk patients proceeding to coronary artery bypass surgery has increased. The most common risk factors are older age and the presence of comorbid complaints. We carried out a retrospective study to confirm this new risk profile and to evaluate its impact on surgical results. PATIENTS AND METHOD We analyzed the changing risk profile of 1360 patients who underwent coronary artery bypass surgery in our hospital between 1993 and 2001, divided into three historical cohorts: 1993-1996, 1997-1999 and 2000-2001. The main factors associated with morbidity and mortality were analyzed by logistic regression analysis. The introduction of new operative techniques, such as off-pump surgery and arterial grafting, was also evaluated. RESULTS The patients' risk profile worsened over time: patients were older, comorbid complaints were more common, and ventricular function was poorer. EuroSCORE figures reflected this trend: estimated mortality in the three historical cohorts was 2.0%, 4.0% and 4.2%, respectively (P<.001). However, risk-adjusted mortality, at 3.7%, 2.7% and 1.5%, respectively, decreased (P<.05), and combined overall morbidity and mortality remained stable, at 16.7%, 16.4% and 13.8%, respectively, (P<.39). There was a non-significant tendency for arterial grafting and off-pump surgery to reduce in-hospital morbidity and mortality. CONCLUSIONS The risk profile of patients undergoing surgery has worsened as their mean age has increased and as comorbid complaints have become more prevalent. However, there has been no simultaneous increase in risk-adjusted mortality. The potential benefits of new surgical advances such as off-pump surgery and multiple arterial grafting must be corroborated by future studies.
Antibiofilm activity of certain phytocompounds and their synergy with fluconazole against Candida albicans biofilms.

OBJECTIVES The aim of this study was to evaluate four phytocompounds (cinnamaldehyde, citral, eugenol and geraniol) for their in vitro inhibitory activity against pre-formed biofilms of Candida albicans alone or in combination with fluconazole and amphotericin B. These compounds were also tested at subinhibitory concentrations for their ability to inhibit biofilm formation. METHODS The XTT reduction assay, light microscopy and scanning electron microscopy (SEM) were employed to determine the inhibitory effect of the test compounds on biofilms. A chequerboard method was used for combination studies. RESULTS Both clinical and reference strains of C. albicans (C. albicans 04 and C. albicans SC5314, respectively) displayed formation of strong biofilms. Pre-formed Candida biofilms showed ≥1024× increased resistance to antifungal drugs and 2× increased resistance to cinnamaldehyde and geraniol, but no increased tolerance of eugenol. The test compounds were more active against pre-formed biofilms than amphotericin B and fluconazole. At 0.5× MIC, eugenol and cinnamaldehyde were the most inhibitory compounds against biofilm formation. Light and electron microscopic studies revealed the deformity of three-dimensional structures of biofilms formed in the presence of sub-MICs of eugenol and cinnamaldehyde. The cell membrane appeared to be the target site of compounds in both planktonic and sessile C. albicans cells, as observed by SEM. Combination studies showed that synergy was highest between eugenol and fluconazole (fractional inhibitory concentration index = 0.14) against pre-formed biofilms of C. albicans SC5314. CONCLUSIONS Promising antibiofilm activity was displayed by eugenol and cinnamaldehyde, which also showed synergy with fluconazole in vitro. Further evaluation in in vivo systems is required to determine whether these findings can be exploited in treating biofilm-associated candidiasis.
Differentiation and recruitment of IL-22-producing helper T cells stimulated by pleural mesothelial cells in tuberculous pleurisy.

RATIONALE IL-22-producing helper T cells (Th22 cells) have been reported to be involved in tuberculosis infection. However, differentiation and immune regulation of Th22 cells in tuberculous pleural effusion (TPE) remain unknown. OBJECTIVES To elucidate the mechanism by which Th22 cells differentiate and recruit into the pleural space. METHODS The distribution and phenotypic features of Th22 cells in both TPE and blood were determined. The impacts of proinflammatory cytokines and antigen presentation by pleural mesothelial cells (PMCs) on Th22-cell differentiation were explored. The chemoattractant activity of chemokines produced by PMCs for Th22 cells was observed. MEASUREMENTS AND MAIN RESULTS Th22 cells were significantly higher in TPE than in blood. IL-1β, IL-6, and/or tumor necrosis factor-α promoted Th22-cell differentiation from CD4(+) T cells. It was found that PMCs expressed CCL20, CCL22, and CCL27, and that TPE and PMC supernatants were chemotactic for Th22 cells. This activity was partly blocked by anti-CCL20, anti-CCL22, and anti-CCL27 antibodies. IL-22 and IL-17 significantly improved PMC wound healing. Moreover, PMCs were able to stimulate CD4(+) T-cell proliferation and Th22-cell differentiation by presenting tuberculosis-specific antigen. CONCLUSIONS The overrepresentation of Th22 cells in TPE may be due to pleural cytokines and to PMC-produced chemokines. Our data suggest a collaborative loop between PMCs and Th22 cells in TPE. In particular, PMCs were able to function as antigen-presenting cells to stimulate CD4(+) T-cell proliferation and Th22-cell differentiation.
Timing of removal of the laryngeal mask airway

Previous studies reported that complications associated with removal of the laryngeal mask were more frequent in awake patients than in anaesthetised patients; however, these studies did not comply with the method described in the manufacturer's instruction manual. The reported incidences of regurgitation during the use of the laryngeal mask also differ considerably between studies. We studied these factors in 66 patients in whom the method described in the manual was used. After induction of anaesthesia, the laryngeal mask and a pH probe were inserted and the cuff of the mask was inflated with a minimum volume of air. Anaesthesia was maintained with nitrous oxide and isoflurane in oxygen. At the end of the operation, we randomly allocated patients to one of two groups and the laryngeal mask was removed either while they were still deeply anaesthetised or after they had regained consciousness. No apparent regurgitation occurred in any patient during operation, but one patient in the anaesthetised group regurgitated immediately after removal of the mask. The incidence of complications during or after removal of the laryngeal mask was significantly greater in the anaesthetised group than that in the awake group (p << 0.001; difference [95% CI]: 48.5 [30.5–66.5]%). Therefore, the laryngeal mask can be safely left in place until the patient has regained consciousness after emergence from anaesthesia.
Testosterone and sex related physical characteristics during the maturation of the male Japanese quail (coturnix coturnix japonica).

A series of experiments was conducted to examine hormonal and physical aspects of reproduction in the maturing and adult male Japanese Quail. Testicular secretion of testosterone was demonstrated in the adult male by the elevated testosterone concentration in the testicular vein (12 ng/ml serum) as compared to the testosterone concentration in the left ventricle (4.5 ng/ml serum). Testosterone concentrations in the peripheral circulation were found to vary diurnally with high concentrations in the morning and evening and lowered concentrations in the midafternoon. In the maturing male, testosterone concentrations, testicular weight and cloacal gland area remained low until Day 21 and rose rapidly thereafter. Spermatozoa were first detected in the testes on Day 26. By 30 days of age, spermatozoa were found in the testes and in the vasa deferentia in small quantities and by Day 35 there were large concentrations at both sites. There was a significant positive temporal correlation of changes in cloacal gland area, testes weight and circulating concentrations of testosterone.
The Extended Symmetry Lie Algebra and the Asymptotic Expansion of the Transversal Correlation Function for the Isotropic Turbulence

The extended symmetry of the functional of length determined in an affine space of the correlation vectors for homogeneous isotropic turbulence is studied. The two-point velocity-correlation tensor field (parametrized by the time variable ) of the velocity fluctuations is used to equip this space by a family of the pseudo-Riemannian metrics (Grebenev and Oberlack (2011)). First, we observe the results obtained by Grebenev and Oberlack (2011) and Grebenev et al. (2012) about a geometry of the correlation space and expose the Lie algebra associated with the equivalence transformation of the above-mentioned functional for the quadratic form generated by which is similar to the Lie algebra constructed by Grebenev et al. (2012). Then, using the properties of this Lie algebra, we show that there exists a nontrivial central extension wherein the central charge is defined by the same bilinear skew-symmetric form as for the Witt algebra which measures the number of internal degrees of freedom of the system. For the applications in turbulence, as the main result, we establish the asymptotic expansion of the transversal correlation function for large correlation distances in the frame of .
Hemicelluloses of wheat straw.

Various hemicellulose fractions were extracted from wheat straw holocellulose (extractive and pectin free) by successive treatments with cold and hot water, 0.5%, 1.0%, and 2.0% potassium hydroxide and were recovered by precipitation with alcohol. Approximately 25% of the holocellulose material was removed, one half being in the hot water soluble fraction. The original holocellulose, the extracted residue, and the recovered fractions were analyzed for pentosan, uronic acid anhydride, acetyl, methoxyl, and ash content. In general, the more soluble fractions had a higher uronic acid and methoxyl content; the less soluble had a higher pentosan content and a more negative rotation . Intrinsic viscosity measurements indicated that all fractions had a degree of polymerization of 25–30. Hydrolysis of the main fraction yielded D-xylose, L-arabinose, D-glucose; in addition D-galactose was found in the water soluble fractions. Quantitative determinations of the sugars in the hydrolyzates showed that D-xylose predom.
Distribution Transformer No-Load Losses

This paper considers the subject of distribution transformers no-load losses and the factors that influence these losses. This paper reviews methods to correct errors in no-load loss measurement caused by excitation voltage distortion and temperature. These methods depend on the ability to separate no-load losses into its hysteresis and eddy current loss components. Accordingly, methods used to separate the no-load losses into its constituent parts are also reviewed. Comments regardings the application of loss separation to transformer cores, as opposed to Epstein samples, are included to serve as a basis for future investigations.
Interference Management in MIMO Wireless Networks

The scarce and overpopulated radio spectrum is going to present a major barrier to the growth and development of future wireless networks. As such, spectrum sharing seems to be inevitable to accommodate the exploding demand for high data rate applications. A major challenge to realizing the potential advantages of spectrum sharing is interference management. This thesis deals with interference management techniques in noncooperative networks. In specific, interference alignment is used as a powerful technique for interference management. We use the degrees of freedom (DoF) as the figure of merit to evaluate the performance improvement due to the interference management schemes. This dissertation is organized in two parts. In the first part, we consider the K-user multiple input multiple output (MIMO) Gaussian interference channel (IC) with M antennas at each transmitter and N antennas at each receiver. This channel models the interaction between K transmitter-receiver pairs sharing the same spectrum for data communication. It is assumed that the channel coefficients are constant and are available at all nodes prior to data transmission. A new cooperative upper-bound on the DoF of this channel is developed which outperforms the known bounds. Also, a new achievable transmission scheme is provided based on the idea of interference alignment. It is shown that the achievable DoF meets the upper-bound when the number of users is greater than a certain threshold, and thus it reveals the channel DoF. In the second part, we consider communication over MIMO interference and X channels in a fast fading environment. It is assumed that the transmitters obtain the channel state information (CSI) after a finite delay which is greater than the coherence time of the channel. In other words, the CSI at the transmitters becomes outdated prior to being exploited for the current transmission. New transmission schemes are proposed which exploit the knowledge of the past CSI at the transmitters to retrospectively align interference in the subsequent channel uses. The proposed transmission schemes offer DoF gain compared to having no CSI at transmitters. The achievable DoF results are the best known results for