Miracle-dz/UniFAR-FTUs huggingface dataset:
A Between-Subjects Test of the Lower-Identification/Higher-Priming Paradox

An under-recognised U-shaped model states that unconscious and conscious perceptual effects are functionally exclusive and that unconscious perceptual effects manifest themselves only at the objective detection threshold, when conscious perception is completely absent. We tested the U-shaped line model with a between-subjects paradigm. Angry, happy, neutral faces, or blank slides were flashed for 5.5 ms and 19.5 ms before Chinese ideographs in a darkened room. A group of volunteers (n = 84) were asked to rate how much they liked each ideograph and performed an identification task. According to the median identification score two subgroups were composed; one with 50% or <50% identification scores (n = 31), and one with above 50% identification scores (n = 53). The hypothesised U-shaped line was confirmed by the findings. Affective priming was found only at the two extreme points: the 5.5 ms condition of the low-identification group (subliminal perception) and the 19.5 ms condition of the >50% high-identification group (supraliminal perception). The two intermediate points (19.5 ms of the low-identification group and 5.5 ms of the high-identification group) did not correspond to significant priming effects. These results confirm that a complete absence of conscious perception is the condition for the deployment of unconscious perceptual effects.
Correlation of lipidomic composition of cell lines and tissues of breast cancer patients using hydrophilic interaction liquid chromatography/electrospray ionization mass spectrometry and multivariate data analysis.

RATIONALE The goal of this work is the comparison of differences in the lipidomic compositions of human cell lines derived from normal and cancerous breast tissues, and tumor vs. normal tissues obtained after the surgery of breast cancer patients. METHODS Hydrophilic interaction liquid chromatography/electrospray ionization mass spectrometry (HILIC/ESI-MS) using the single internal standard approach and response factors is used for the determination of relative abundances of individual lipid species from five lipid classes in total lipid extracts of cell lines and tissues. The supplementary information on the fatty acyl composition is obtained by gas chromatography/mass spectrometry (GC/MS) of fatty acid methyl esters. Multivariate data analysis (MDA) methods, such as nonsupervised principal component analysis (PCA), hierarchical clustering analysis (HCA) and supervised orthogonal partial least-squares discriminant analysis (OPLS-DA), are used for the visualization of differences between normal and tumor samples and the correlation of similarity between cell lines and tissues either for tumor or normal samples. RESULTS MDA methods are used for differentiation of sample groups and also for identification of the most up- and downregulated lipids in tumor samples in comparison to normal samples. Observed changes are subsequently generalized and correlated with data from tumor and normal tissues of breast cancer patients. In total, 123 lipid species are identified based on their retention behavior in HILIC and observed ions in ESI mass spectra, and relative abundances are determined. CONCLUSIONS MDA methods are applied for a clear differentiation between tumor and normal samples both for cell lines and tissues. The most upregulated lipids are phospholipids (PL) with a low degree of unsaturation (e.g., 32:1 and 34:1) and also some highly polyunsaturated PL (e.g., 40:6), while the most downregulated lipids are PL containing polyunsaturated fatty acyls (e.g., 20:4), plasmalogens and ether lipids. Copyright © 2016 John Wiley & Sons, Ltd.
The ‘Alternative für Deutschland in the Electorate’: Between Single-Issue and Right-Wing Populist Party

The good result of the recently formed Alternative für Deutschland (AfD) was a striking outcome of the 2013 Federal Election. This article explores why AfD supporters chose this party at the 2013 Federal Election and at the 2014 European and eastern German State (Land) Elections. At the Federal Election the AfD's electorate was composed of two groups: a minority of instrumental issue-voters that were drawn to the AfD by its emphasis and positioning on the Euro crisis, and a majority of ‘late supporters’ that decided close to Election Day and were moved more by expressive motives, most notably xenophobic sentiments like those identified in other European countries as a main source of support for right-wing populist parties. The analysis of the subsequent elections shows that, paralleling developments in the AfD's public rhetoric, the Euro crisis ceased to be important for AfD support whereas xenophobic motives became more central.
Giant renal angiomyolipoma

A 65-year-old woman from a rural area presented to the surgical service with a very large abdominal lump that the patient had first noticed 8 years previously. The lump had steadily increased in size over time, and the patient had dysuria, weakness and dyspnoea on exertion. On examination she was grossly anaemic with a large retroperitoneal lump that occupied the whole right side of abdomen; the lump was soft to firm in consistency and non-tender. She had no lymphoedema or other palpable lymph nodes. Ultrasound revealed a large retroperitoneal tumour with fatty elements, arising from the right kidney. There was no involvement of the intestines. Findings of the computed tomography scan were typical for renal angiomyolipoma. The patient underwent surgery and the tumour was excised. Four units of blood were transfused preoperatively and in addition two units were given postoperatively. The patient was given thromboprophylaxis perioperatively and in the postoperative period because she was bedridden and could not ambulate. She made an uneventful recovery.
Molecular Events in the Growth Inhibition of Bacillus subtilis by d-Tyrosine

The transformable strain of Bacillus subtilis strain 168 is extremely susceptible to growth inhibition by d-tyrosine. The molecular events associated with the inhibition of growth by d-tyrosine in this strain include the false feedback inhibition and probably the false repression of prephenate dehydrogenase. These effects were found to contribute to the formation of d-tyrosine-containing proteins by decreasing the intracellular concentration of l-tyrosine. Accordingly, growth inhibition of strain 168 by the d isomer of tyrosine was shown to be progressive, enduring, and delayed by prior growth on l-tyrosine. The synthesis of cellular macromolecules and viable cell count were progressively diminished in d-tyrosine-inhibited cultures. Several different enzyme activities were reduced after growth in the presence of d-tyrosine. Isotopic d-tyrosine was incorporated into cellular proteins without change of optical configuration. Long chains of cells with completed septa were observed microscopically, and therefore some cell wall effect may also be implicated.
Bacillus anthracis sin Locus and Regulation of Secreted Proteases

ABSTRACT Bacillus anthracis shares many regulatory loci with the nonpathogenic Bacillus species Bacillus subtilis. One such locus is sinIR, which in B. subtilis controls sporulation, biofilm formation, motility, and competency. As B. anthracis is not known to be motile, to be naturally competent, or to readily form biofilms, we hypothesized that the B. anthracis sinIR regulon is distinct from that of B. subtilis. A genome-wide expression microarray analysis of B. anthracis parental and sinR mutant strains indicated limited convergence of the B. anthracis and B. subtilis SinR regulons. The B. anthracis regulon includes homologues of some B. subtilis SinR-regulated genes, including the signal peptidase gene sipW near the sinIR locus and the sporulation gene spoIIE. The B. anthracis SinR protein also negatively regulates transcription of genes adjacent to the sinIR locus that are unique to the Bacillus cereus group species. These include calY and inhA1, structural genes for the metalloproteases camelysin and immune inhibitor A1 (InhA1), which have been suggested to be associated with virulence in B. cereus and B. anthracis, respectively. Electrophoretic mobility shift assays revealed direct binding of B. anthracis SinR to promoter DNA from strongly regulated genes, such as calY and sipW, but not to the weakly regulated inhA1 gene. Assessment of camelysin and InhA1 levels in culture supernates from sinR-, inhA1-, and calY-null mutants showed that the concentration of InhA1 in the culture supernatant is inversely proportional to the concentration of camelysin. Our data are consistent with a model in which InhA1 protease levels are controlled at the transcriptional level by SinR and at the posttranslational level by camelysin.
Induction of IL-10+CD4+CD25+ T cells by grass pollen immunotherapy.

BACKGROUND Immunotherapy involves the modulation of allergen-specific T-cell responses, either T(H)2-to-T(H)1 immune deviation or, in bee venom-treated patients, induction of IL-10 production by CD4+CD25+ T cells. IL-10-producing CD4+CD25+ regulatory T cells have emerged as potential mediators of immune tolerance in numerous murine models of immunopathology. OBJECTIVE The aim of this study was to evaluate the role of IL-10 production and CD4+CD25+ T cells in the response to grass pollen immunotherapy. METHODS PBMCs were isolated from patients after 1 year of grass pollen immunotherapy and from matched untreated atopic and healthy control subjects. After 6 days of in vitro stimulation with Phleum pratense, production of IL-10, IL-5, IL-4, and IFN-gamma and proliferation and numbers of CD4+CD25+ T cells were measured. T cells were then stimulated for a further 5 hours with phorbol 12-myristate 13-acetate and ionomycin and assessed for intracellular IL-10 by means of flow cytometry. RESULTS Patients undergoing immunotherapy produced significantly more IL-10 than atopic control subjects (patients undergoing immunotherapy, 116 +/- 21 pg/mL [n = 11]; atopic patients, 30 +/- 5 pg/mL [n = 11]; P <.001), and the number of CD4+CD25+ cells identified after allergen stimulation was also greater in the immunotherapy group. The numbers of CD4+CD25+ T cells correlated positively with activation as measured by proliferation in both of the control groups but not in the immunotherapy group. Moreover, only T cells from patients undergoing immunotherapy were positive for intracellular IL-10, and these were almost exclusively CD4+CD25+ cells. CONCLUSION Grass pollen immunotherapy results in a population of circulating T cells that express the IL-10(+) CD4+CD25+ phenotype in response to allergen restimulation.
Serum Levels of Calcification Inhibition Proteins and Coronary Artery Calcium Score: Comparison between Transplantation and Dialysis

Vascular calcifications in CKD are now linked to serum alterations of both divalent ions and calcification inhibitory proteins. Due to possible biochemical differences between dialysis (D) and transplantation (Tx), we examined the entity and severity of these biochemical modifications and of coronary artery calcium score separately in these two populations. We assayed, besides standard markers of inflammation, divalent ions and serum levels of fetuin, matrix Gla protein (MGP) and osteoprotegerin (OPG), in 51 Tx patients (age 45 ± 12 years; 30 males, 21 females; previous D duration 4.8 ± 4.2 years; Tx since 6.6 ± 5.5 years; Cr 1.8 ± 0.6 mg/dl) and in 49 D patients (age 49 ± 14 years; 30 males,19 females; D duration 5.6 ± 4.8 years). Additionally, coronary calcium score (AS) was evaluated by cardiac multi-slice CT. Compared with D patients, Tx patients had better values of divalent ions and inflammation markers, and lower prevalence (65 vs. 86%; p < 0.02) and severity (AS = 570 ± 1,637 vs. 1,311 ± 3,128; p < 0.008) of coronary calcification. In addition, a tendency toward normalization for all of the three calcification inhibitory proteins was evident. In both Tx and D, AS correlated with age and OPG (Tx: rs = 0.439, p < 0.001, and rs = 0.510, p < 0.0001; D: rs = 0.471, p < 0.001, and rs = 0.403, p < 0.005, respectively); in D patients, a correlation was present also with D duration (rs = 0.435; p < 0.002), other markers of inflammation and, notably, fetuin (rs = –0.442; p < 0.002). Regression analysis selected previous time on D in Tx patients (rm = 0.400; p < 0.004), and C-reactive protein and OPG in D patients (rm = 0.518; p < 0.004) as the most predictive parameters of AS. Discriminant analysis confirmed the major role of age and D duration in the appearance of AS and evidenced male gender as a distinct risk condition. At variance, Tx duration was never associated with AS. In conclusion, as compared to D, renal Tx patients show serum levels of calcification inhibition proteins and of divalent ions closer to normal. As this is associated with a lower prevalence and severity of AS, it is suggested that Tx antagonize the accelerating role of D in the progression of vascular calcification. Assessment of both coronary calcifications and serum levels of calcification inhibitory proteins may be of value to identify those subjects at higher risk of development and progression of vascular lesions, among whom males have the highest rate.
Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains

Abstract Talà, A. , Belloni, F. , Monaco, C. , Lorusso, A. , Nassisi, V. and Alifano, P. Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains. Radiat. Res. 165, 532–537 (2006). recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV308 nm coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV254 nm germicidal lamp. Compared to the germicidal lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was consistent with previous data on residual biochemical activities of the respective mutated RecA proteins in vitro. The discrepancy between the results obtained with the lamp and laser irradiation suggested that the biological response to the two radiations involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV308 nm radiation induced mutagenesis in recA-defective strains at an extent considerably greater than in recA-proficient strains. In contrast, far-UV254 nm-radiation-induced mutagenesis was reported to be largely dependent on a functional recA allele.
Conductance features in point contact Andreev reflection spectra

Point contact Andreev reflection (PCAR) spectroscopy is a common technique for determining the spin polarization of a ferromagnetic sample. The polarization is extracted by measuring the bias dependence of the conductance of a metallic/superconducting point contact. Under ideal conditions, the conductance is dominated by Andreev reflection and the Blonder–Tinkham–Klapwijk (BTK) model can be used to extract a value for the polarization. However, PCAR spectra often exhibit unwanted features in the conductance that cannot be appropriately modelled with the BTK theory. In this paper we isolate some of these unwanted features and show that any further extraction of the spin polarization from these non-ideal spectra proves unreliable. Understanding the origin of these features provides an objective criterion for rejection of PCAR spectra unsuitable for fitting with the modified BTK model.
Paraganglioma of the Urinary Bladder: A Case Report and Review of the Japanese Literature

We describe a patient with primary paraganglioma of the urinary bladder associated with typical clinical symptoms. Systolic blood pressure frequently increased to 300 mm Hg immediately after micturition. Levels of urinary vanillylmandelic acid excretion and serum norepinephrine were significantly elevated. The tumor was removed by partial cystectomy. Histological examination of the tumor revealed paraganglioma of the urinary bladder. Compared with a review of 53 similar cases reported in Japan, the distribution of age, sex and tumor location in the bladder differed from those described worldwide.
Enhanced Production of Human Mini‐Proinsulin in Fed‐Batch Cultures at High Cell Density of Escherichia coli BL21(DE3) [pET‐3aT2M2]

Synthesis of recombinant protein (human mini‐proinsulin) is investigated in fed‐batch cultures at high cell concentration of recombinant Escherichia coli BL21(DE3) [pET‐3aT2M2]. Transcription of the recombinant gene is controlled by a T7 promoter system. The human mini‐proinsulin is characterized by a C‐chain peptide consisting of only nine amino acids, whereas the C‐chain peptide of natural human proinsulin is made up of 35 amino acids. It is expressed in a fusion protein with a small fusion partner (a peptide with 18 amino acids) and finally aggregated into insoluble inclusion bodies in cytoplasm of recombinant E. coli. The fermentative production of this small fusion mini‐proinsulin may be of great advantage in enhancing the yield of human insulin. To find an optimum induction strategy, effects of various key cultivation variables on the mini‐proinsulin production are examined in high cell density fed‐batch cultures. No general correlation is found between preinduction specific growth rate and recombinant protein synthesis, which confers a flexibility in choosing the feeding strategy of preinduction media for achieving the high cell density cultures. A culture temperature below 37 °C is unfavorable for recombinant gene expression, and the T7‐based expression system is almost completely repressed at 30 °C. The nutrient glucose and yeast extract concentration in postinduction feed media is optimized by applying a statistical method for medium optimization, i.e. response surface methodology, and an effective amount of inducer molecule (IPTG) is determined to maximize the specific recombinant protein formation. The mini‐proinsulin production in E. coli culture is significantly influenced by the volumetric feed rate of postinduction media, which is shown to be closely related to the plasmid copy number in the recombinant cell. Consequently, in a single‐stage fed‐batch process, the mini‐proinsulin concentration is increased up to 7 g/L, approximately 62 wt % of which corresponds to mature human insulin. A two‐stage fed‐batch fermentation process, with recombinant cell growth occurring at a constant growth rate and constant cell concentration in a growth fermenter and mini‐proinsulin production in an induction fermenter, is designed, and its efficacy in increasing volumetric productivity of mini‐proinsulin is demonstrated.
Design and verification of SystemC transaction-level models

Transaction-level modeling allows exploring several SoC design architectures, leading to better performance and easier verification of the final product. In this paper, we present an approach to design and verify SystemC models at the transaction level. We integrate the verification as part of the design flow where we first model both the design and the properties (written in Property Specification language) in Unifed Modeling Language (UML); then, we translate them into an intermediate format modeled with AsmL [language based on Abstract State Machines (ASM)]. The AsmL model is used to generate a finite state machine of the design, including the properties. Checking the correctness of the properties is performed on the fly while generating the state machine. Finally, we translate the verified design to SystemC and map the properties to a set of assertions (as monitors in C#) that can be reused to validate the design at lower levels by simulation. For existing SystemC designs, we propose to translate the code back to AsmL in order to apply the same verification approach. At the SystemC level, we also present a genetic algorithm to enhance the assertions coverage. We will ensure the soundness of our approach by proving the correctness of the SystemC-to-AsmL and AsmL-to-SystemC transformations. We illustrate our approach on two case studies including the PCI bus standard and a master/slave generic architecture from the SystemC library.
Evidence Implicating Eating as a Primary Driver for the Obesity Epidemic

This article addresses the extent to which increases in energy intake as opposed to decreases in energy expenditure are driving the obesity epidemic. It argues that while both intake and expenditure are plausible and probable contributors, the fact that all intake is behavioral, whereas less than half of expenditure is behavioral, makes intake a conceptually more appealing primary cause. A review of per capita food disappearance trends over time and of trends in individual intakes is presented to support the plausibility of this perspective. Increases in energy intake mirror increases in body weight quantitatively and are equally widely distributed across diverse groups within the larger population.
Effect of Liquid to Solid Ratio on Autohydrolysis of Eucalyptus globulus Wood Meal

Autohydrolysis of Eucalyptus globulus both with and without the addition of 5 g/L formic acid was explored for different liquid-to-solid (L/S) ratios at 150 oC for 100 min. The L/S ratio has an impact on the dissolution of wood components during prehydrolysis. The extraction yield of wood components other than lignin decreased with increasing L/S ratio, while lignin removal increased with increasing L/S ratio, irrespective of acid reinforcement. The molecular weight (Mw) of dissolved hemicelluloses and lignin remained relatively constant. The average degree of polymerization of hemicelluloses isolated from the hot water extract was between 7 and 8 over a L/S ratio range from 3:1 to 50:1. The cellulose to glucose conversion yield of pretreated wood samples improved during prehydrolysis with 5 g/L formic acid. Although the conversion yield of autohydrolyzed wood meal stayed relatively constant, the conversion yield of dilute acid-pretreated wood samples increased with increasing L/S ratio.
An in situ probe for on‐line monitoring of cell density and viability on the basis of dark field microscopy in conjunction with image processing and supervised machine learning

Fermentation industries would benefit from on‐line monitoring of important parameters describing cell growth such as cell density and viability during fermentation processes. For this purpose, an in situ probe has been developed, which utilizes a dark field illumination unit to obtain high contrast images with an integrated CCD camera. To test the probe, brewer's yeast Saccharomyces cerevisiae is chosen as the target microorganism. Images of the yeast cells in the bioreactors are captured, processed, and analyzed automatically by means of mechatronics, image processing, and machine learning. Two support vector machine based classifiers are used for separating cells from background, and for distinguishing live from dead cells afterwards. The evaluation of the in situ experiments showed strong correlation between results obtained by the probe and those by widely accepted standard methods. Thus, the in situ probe has been proved to be a feasible device for on‐line monitoring of both cell density and viability with high accuracy and stability. Biotechnol. Bioeng. 2007;97:1489–1500. © 2007 Wiley Periodicals, Inc.