Miracle-dz/UniFAR-FTUs huggingface dataset:
Simian Virus 40 Vp1 DNA-Binding Domain Is Functionally Separable from the Overlapping Nuclear Localization Signal and Is Required for Effective Virion Formation and Full Viability

ABSTRACT A DNA-binding domain (DBD) was identified on simian virus 40 (SV40) major capsid protein Vp1, and the domain's function in the SV40 life cycle was examined. The DBD was mapped by assaying various recombinant Vp1 proteins for DNA binding in vitro. The carboxy-terminal 58-residue truncated Vp1ΔC58 pentamer bound DNA with aKd of 1.8 × 10−9 M in terms of the protein pentamer, while full-length Vp1 and carboxy-terminal-17-truncated Vp1ΔC17 had comparable apparentKd s of 5.3 × 10−9 to 7.3 × 10−9 M in terms of the protein monomers. Previously identified on Vp1 was a nuclear localization signal (NLS) consisting of two N-terminal basic clusters, NLS1 (4-KRK-6) and NLS2 (15-KKPK-18). Vp1ΔC58 pentamers harboring multiple-point mutations in NLS1 (NLSm1), NLS2 (NLSm2), or both basic clusters (NLSm1 · 2) had progressively decreased DNA-binding activity, down to 0.7% of the Vp1ΔC58 level for NLSm1 · 2 Vp1. These data, along with those of N-terminally truncated proteins, placed the DBD in overlap with the bipartite NLS. The role of the Vp1 DBD during infection was investigated by taking advantage of NLS phenotypic complementation (N. Ishii, A. Nakanishi, M. Yamada, M. H. Macalalad, and H. Kasamatsu, J. Virol. 68:8209–8216, 1994), in which an NLS-defective Vp1 could localize to the nucleus in the presence of wild-type minor capsid proteins Vp2 and Vp3. This approach made it possible to dissect the role of the bifunctional Vp1 NLS-DBD in virion assembly in the nucleus. Mutants of the viable nonoverlaping SV40 (NO-SV40) DNA NLSm1, NLSm2, and NLSm1 · 2 replicated normally following transfection into host cells and produced capsid proteins at normal levels. All mutant Vp1s were able to interact with Vp3 in vitro. The mutants NLSm1 and NLSm1 · 2 were nonviable, and the mutant Vp1s unexpectedly failed to localize to the nucleus though Vp2 and Vp3 did, suggesting that the mutated NLS1 acted as a dominant signal for the cytoplasmic localization of Vp1. Mutant NLSm2, for which the mutant Vp1's nuclear localization defect was complemented by Vp2 and Vp3, displayed a 5,000-fold reduced viability. Analysis of NLSm2 DNA-transfected cell lysate revealed a 10-fold reduction in the level of DNase I-protected viral DNA, and yet virion-like particles were found among the DNase I-resistant material. Collective results support a role for Vp1 NLS2-DBD2 in the assembly of virion particles. The results also suggest that this determinant can function in the infection of new cells.
Are food intolerances and allergies increasing in immigrant children coming from developing countries ?

There are not available data concerning the occurrence, the clinical features and the environmental risk factors for food intolerances and allergies in immigrant children. The aim of the study was to evaluate rates, distribution, clinical features and environmental risk factors for food intolerances and allergies in immigrant children. Hospital records of 4130 patients with celiac disease (CD), cow milk protein intolerance (CMPI) and food allergies (FA) diagnosed in 24 Italian Centres from 1999 to 2001 were retrospectively reviewed, comparing immigrant patients with Italian ones. 78/4130 (1.9%) patients were immigrant: 36/1917 (1.9%) had CD, 24/1370 (1.75%) CMPI and 18/843 (2.1%) FA. They were evenly distributed across Italy and their native areas were: East Europe (23/78), Northern Africa (23/78), Southern Asia (14/78), Saharan and Sub‐Saharan Africa (9/78), Southern America (4/78), Far East (3/7), Middle East (2/78). Despite differences in their origin, the clinical features of immigrant children were similar to the ones of Italian patients and among each ethnic group. The majority of them were born in Italy (57/78) or have been residing in Italy since several years (19/78). All of them had lost dietary habits of the native countries and had acquired those of the Italian childhood population. Food intolerances and allergies are present also in children coming from developing countries, and paediatricians will need to have a full awareness of them because the number of immigrant children in Italy is quickly increasing. The clinical features of food intolerances and allergies appear the same in each ethnic group, despite differences in races. Sharing of dietary habits with the Italian childhood population seems to be an important environmental risk factor.
Application Research on " Flipped Classroom " Teaching Mode in Colleges and Universities

"Flipped classroom" as a new form of teaching break through the limitations of traditional classroom teaching, to reconstruct the new idea of teaching structure and teaching, this paper study in order to promote the application of "Flipped classroom". The research mainly includes three aspects: one is that of the traditional classroom and "Flipped classroom" contrast analysis; Tow it is, "Flipped classroom" teaching model learning task design, designed by the study guide, learning task, question design, constructive learning resources, learning test, archives and the reflection of "Learning task list"; Three, put forward a "Flipped classroom" teaching mode. In this paper, the research results, to comprehensively promote quality education, promote the development of higher education provides a new solution.
PENILE PLETHYSMOGRAPHY: STRENGTHS, LIMITATIONS, INNOVATIONS1

Dorland’s Illustrated Medical Dictionary (1994) defines plethysmography as ‘the recording of the changes in the size of a [body] part as modified by the circulation of the blood in it’ (p. 1306). This is done by measuring electrical impedance, ‘a technique for detecting blood volume changes in a part by measuring changes in electrical resistance’. Impedance changes are detected by a strain gauge, ‘a technique for detecting blood volume changes in . circumference employing a rubber tube filled with a conductive liquid; as the tube expands and contracts, the resistance in the fluid changes in proportion to the circumference’. A plethysmograph is ‘an instrument for determining and registering variations in the volume of an organ’ (Dorland’s Illustrated Medical Dictionary, 1994, p. 1306, passim). In medicine, plethysmography is primarily used for ‘measuring changes in body volume, used especially in measuring pulmonary ventilation’ as well as for ‘measuring blood volume taking place in a single finger’ (Dorland’s Illustrated Medical Dictionary, 1994, p. 1306). This description shows that it is a small step from measuring blood volume in a single finger to measuring circumferential change in a penis. This impressive definition lends an aura of scientific respectability to penile plethysmography. There are two general methods for measuring changes in penis size. The first, called the volumetric method, was developed by Freund in the former
A Multilingual Perspective on Translanguaging

Translanguaging is a new term in bilingual education; it supports a heteroglossic language ideology, which views bilingualism as valuable in its own right. Some translanguaging scholars have questioned the existence of discrete languages, further concluding that multilingualism does not exist. I argue that the political use of language names can and should be distinguished from the social and structural idealizations used to study linguistic diversity, favoring what I call an integrated multilingual model of individual bilingualism, contrasted with the unitary model and dual competence model. I further distinguish grammars from linguistic repertoires, arguing that bilinguals, like monolinguals, have a single linguistic repertoire but a richly diverse mental grammar. I call the viewpoint developed here a multilingual perspective on translanguaging.
Reproductive ecology, spawning potential, and breeding season of blue swimming crab (Portunidae: Portunus pelagicus) in Java Sea, Indonesia

Ernawati T, Sumiono B, Madduppa H. 2017. Reproductive ecology, spawning potential, and breeding season of blue swimming crab (Portunidae: Portunus pelagicus) in Java Sea, Indonesia. Biodiversitas 18: 1705-1713. The blue swimming crab (Portunidae: Portunus pelagicus) is one of the most economically important fisheries species in Indonesia. Little is known about their reproductive ecology and key biological characteristics. This study aimed to investigate population biology and reproductive ecology (Carapaceâ€™s width to weight relationship, growth, natural mortality, size at first maturity and at first captured, Spawning Potential Ratio (SPR), and breeding season) of P. pelagicus at six important landing sites around the Java Sea (Jakarta, Cirebon, Demak, Rembang, Sumenep, and Sampit) from January 2014 to January 2015. Monthly data were collected by fishers at each landing sites, with a total of 14,408 individuals being measured. The study found that males were heavier than females and the smallest mean of individualsâ€™ height belonged to Jakarta compared to other locations. The growth parameters varied at the different locations. Crabs could grow up to 100 mm in 10 months with the maximum age being less than three years. The growth rate (K) of more than 1 indicated a fast-growing crustacean with a short lifespan. The lowest carapace width at first capture (Lc) for the BSC was obtained from crabs caught using a bottom gillnet landed in Jakarta, followed by dredged crabs in Cirebon. The mean carapace width at first maturity (Lm) in the Java Sea was 10.6 cm, with the lowest Lm of female crabs being landed in Cirebon and the highest in Sampit. The Spawning Potential Ratio (SPR) ranged from 11% to 24% and the selectivity curve for the BSC fishery at each landing sites is positioned well to the right of the generic maturity curve, except in Demak, suggests that the operation enables almost all crabs to mature and spawn before entering the fishery. The collapsible trap was the most selective gear, with an average catch of 112 mm across at all landing sites, and the least selective was in trawls. Therefore consideration needs to be given to the different gear types used to catch crabs to leave smaller crabs in the water longer to grow to maturity. Considering its fast growth and peak season (February-April and August-October), a short-period is recommended at some sites that have been over-exploited to rebuild a broodstock to support the long-term sustainability of this species.
Medical treatment for sphincter of oddi dysfunction: can it replace endoscopic sphincterotomy?

AIM To report the results of a medical management of sphincter of oddi dysfunction (SOD) after an intermediate follow-up period. METHODS A total of 59 patients with SOD (2 men and 57 women, mean age 51 years old) were included in this prospective study. After medical treatment for one year, the patients were clinically re-evaluated after an average period of 30 mo. RESULTS The distribution of the patients according to the Milwaukee's classification was the following: 11 patients were type 1, 34 were type 2 and 14 were type 3. Fourteen patients underwent an endoscopic sphincterotomy (ES) after one year of medical treatment. The median intermediate follow-up period was 29.8 ± 3 mo (3-72 mo). The initial effectiveness of the medical treatment was complete, partial and poor among 50.8%, 13.5% and 35%, respectively, of the patients. At the end of the follow-up period, 37 patients (62.7%) showed more than 50% improvement. The rate of improvement in patients who required ES was not significantly different compared with the patients treated conservatively (64.2% vs 62.2%, respectively). CONCLUSION Our study confirms that conservative medical treatment could be an alternative to endoscopic sphincterotomy because, after an intermediate follow-up period, the two treatments show the same success rates.
Knee Joint Biomechanical Gait Data Classification for Knee Pathology Assessment: A Literature Review

Background The purpose of this study is to review the current literature on knee joint biomechanical gait data analysis for knee pathology classification. The review is prefaced by a presentation of the prerequisite knee joint biomechanics background and a description of biomechanical gait pattern recognition as a diagnostic tool. It is postfaced by discussions that highlight the current research findings and future directions. Methods The review is based on a literature search in PubMed, IEEE Xplore, Science Direct, and Google Scholar on April 2019. Inclusion criteria admitted articles, written in either English or French, on knee joint biomechanical gait data classification in general. We recorded the relevant information pertaining to the investigated knee joint pathologies, the participants' attributes, data acquisition, feature extraction, and selection used to represent the data, as well as the classification algorithms and validation of the results. Results Thirty-one studies met the inclusion criteria for review. Conclusions The review reveals that the importance of medical applications of knee joint biomechanical gait data classification and recent progress in data acquisition technology are fostering intense interest in the subject and giving a strong impetus to research. The review also reveals that biomechanical data during locomotion carry essential information on knee joint conditions to infer an early diagnosis. This survey paper can serve as a useful informative reference for research on the subject.
A diagnosis for maniacs.

Melanocytes originate in the neural crest and migrateto the basal layer of the epidermis, a distance that is tinyin an embryo but large in an adult human. A little im-precision in regard to where they come to rest may seemto be a small matter. It is not a small matter to a micros-copist who must decide whether or not melanocytes situ-ated in the spinous, granular or cornified layers of theepidermis are evidence of melanoma.In the last two decades there have been a number ofstudies establishing that nevi of “special sites” may haveeccentricities that, if they were detected elsewhere wouldbe evidence of melanoma. Nevi on the genitalia featurelarge and sometimes laterally confluent junctional nests,often of melanocytes with pale staining cytoplasm anddense lymphocytic infiltrates (1). Those on the umbilicusare altered by the presence of a scar in the dermis. Nevion the conjunctiva present with even more striking con-fluence of nests than genital nevi have, with clefts be-tween the epithelium and submucosa and dense infil-trates of lymphocytes, sometimes with lymphoid folliclesand eosinophils. Pigmentation may be prominent in thedeepest nests. Nevi in the neck, axillae and groins canresemble skin tags clinically and have a polypoid con-figuration microscopically (2). Of all of the nevi on “special sites”, those on the skinof the acra are most easily confused with melanoma byvirtue of suprabasal scatter of melanocytes. Thesechanges are most notable on nevi from the palms, solesand nailbeds, but also occur on the dorsal surfaces ofhands and feet and on knees and elbows as well. When Ibegan in dermatopathology in the early 1980s, this factwas little known. Many nevi on acral skin were labeledas melanoma or melanoma in situ, or with such terms as“atypical acral melanosis” that were often joined to notessuggesting re-excision. I wondered how it could be thatearly lesions of melanoma in situ could be so commonyet acral melanomas so are so rare, and seldom associ-ated with pre-existent nevi. I called a podiatric patholo-gist and a local dermatopathologist in private practicewho both told me that they routinely saw, but ignored thefinding of pagetoid scatter of melanocytes based on theexperience of seeing it so often. Clearly, some people
THE 31 ST OCTOBER 2002 EARTHQUAKE IN MOLISE ( ITALY ) : A NEW METHODOLOGY FOR THE DAMAGE AND SEISMIC VULNERABILITY SURVEY OF CHURCHES

This paper describes the new methodology used to assess seismic damage in the churches of Molise damaged by the 31 October 2002 seismic event. In the paper the first results of the statistical reelaboration of the data collected with the new survey form are presented, which is based on the method used after the 1997 earthquake in Umbria and The Marches (Lagomarsino [1]). The possibility of having a vulnerability survey of the churches through a subdivision into vulnerability indicators and a-seismic devices has allowed us a more accurate evaluation of the seismic behaviour of this kind of building that showed considerable damage, despite the low magnitude of the two main seismic events. The high intrinsic vulnerability of churches, , has, in fact, in many cases been increased by not so effective retrofitting interventions. The new detailed form has allowed us to identify these specific aspects and to give meaningful suggestions for the successive reconstruction phase.
A Rapid One-Pot Synthesis of Novel High-Purity Methacrylic Phosphonic Acid (PA)-Based Polyhedral Oligomeric Silsesquioxane (POSS) Frameworks via Thiol-Ene Click Reaction

Herein, we demonstrate a facile methodology to synthesis a novel methacrylic phosphonic acid (PA)-functionalized polyhedral oligomeric silsesquioxanes (POSSs) via thiol-ene click reaction using octamercapto thiol-POSS and ethylene glycol methacrylate phosphate (EGMP) monomer. The presence of phosphonic acid moieties and POSS-cage structure in POSS-S-PA was confirmed by Fourier transform infrared (FT-IR) and nuclear magnetic resonance (1H, 29Si and 31P-NMR) analyses. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrum of POSS-S-PA acquired in a dithranol matrix, which has specifically designed for intractable polymeric materials. The observed characterization results signposted that novel organo-inorganic hybrid POSS-S-PA would be an efficacious material for fuel cells as a proton exchange membrane and high-temperature applications due to its thermal stability of 380 °C.
DT-CWT AND NLM BASED SATILLITE IMAGE RESOLUTION ENHANCEMENT USING WAVELET-DOMAIN APPROACH

--In this paper, Resolution enhancement (RE) model has one of the biggest disadvantage (drawback) is losing high frequency contents (which results in blurring). Discrete wavelet transform based (DWT) RE scheme generates artifacts due to a DWT shift-variant property. For RE of the satellite images, here, new wavelet domain approach based on dual-tree complex wavelet transform (DT-CWT) and nonlocal means (NLM) is proposed. A satellite input image is decomposed by DT-CWT which is nearly shift invariant to obtain high-frequency sub bands. To interpolate the high-frequency sub bands and the low-resolution (LR) input images; in this paper the Lanczos interpolator is used. The high frequency sub bands are passed through an NLM filter to cater for the artifacts generated by DT-CWT. To obtain a resolution-enhanced image we are combining the filtered high-frequency sub bands and the LR input image by using inverse DT-CWT. Objective and subjective analyses show superiority of the new proposed technique over the conventional and state-of-the-art RE techniques.
RELEVANCE OF R-FACTOR AND FUNDAMENTAL PERIOD FOR SEISMIC DESIGN OF TUNNEL FORM BUILDINGS

In many seismic design codes and guidelines, such as UBC (1997), NEHRP provisions (ASCE 2000) and Turkish Seismic Code (1998), reduction in seismic forces via response modification factor (R) is justified by the unquantified overstrength and ductile response of buildings during design earthquake. However, none of these references address R-factor for RC buildings composed of solely shear walls without accommodating any columns and beams. The typical implementation of such a structural system is reflected in multi-storey reinforced concrete tunnel form buildings. The unique lateral and vertical load transferring mechanism and ease of construction of tunnel form buildings lead them as the prevailing structural type especially in the regions prone to high seismic risk. This paper first addresses the evaluation of a consistent R-factor for their seismic design. Inelastic static pushover analysis was utilized for that purpose on a three-dimensional (3D) highly detailed model. Second, consistency of code-based empirical equations to explicitly estimate the fundamental period of tunnel form buildings was evaluated. The results of analyses show that conventional empirical equations are incapable of predicting the true fundamental period of tunnel form buildings. Based on the premise that such simplified equations are commonly used in practice, a new predictive equation was proposed herein. This equation was developed based on the 3D analyses of 140 tunnel form buildings having a variety of plans, heights and shear-wall configurations. Comparisons with experimental results show good correlation, and lend further credibility to proposed equation for its use in practice.
Antibiotics Susceptibility Pattern of Escherichia coli Isolated from Well Water in Afikpo , South Eastern Nigeria

The aim of this study was to isolate and identify E. coli from well water sources in Afikpo and to determine their antibiotics resistance profiles. Water samples from ten different wells were analysed using standard microbiological and biochemical techniques. Results showed that the total bacterial counts and E. coli counts were all above limits set by World Health Organization. Antibiotics susceptibility studies were conducted using Kirby and Bauer disc diffusion method according to National Committee for clinical Laboratory Standards, (NCCLS). The result of antibiotic studies showed that all the E. coli isolates were 100% resistant to gentamicin and amoxicillin, and 70% resistant to chloramphenicol, while In contrast the isolates exhibited sensitivity to ciprofloxacin, Pefloxacin and tarivid. It is recommended that adequate awareness is created on water quality and sanitation together with construction of protected well to reduce the risk of water-borne diseases in Afikpo area.
Multi-Modal Music Emotion Recognition: A New Dataset, Methodology and Comparative Analysis

We propose a multi-modal approach to the music emotion recognition (MER) problem, combining information from distinct sources, namely audio, MIDI and lyrics. We introduce a methodology for the automatic creation of a multi-modal music emotion dataset resorting to the AllMusic database, based on the emotion tags used in the MIREX Mood Classification Task. Then, MIDI files and lyrics corresponding to a sub-set of the obtained audio samples were gathered. The dataset was organized into the same 5 emotion clusters defined in MIREX. From the audio data, 177 standard features and 98 melodic features were extracted. As for MIDI, 320 features were collected. Finally, 26 lyrical features were extracted. We experimented with several supervised learning and feature selection strategies to evaluate the proposed multi-modal approach. Employing only standard audio features, the best attained performance was 44.3% (F-measure). With the multi-modal approach, results improved to 61.1%, using only 19 multi-modal features. Melodic audio features were particularly important to this improvement.