Miracle-dz/UniFAR-FTUs huggingface dataset:
Histological and immunohistochemical evaluation of two cell therapy protocols in equine suspensory ligament repair

ABSTRACT This study aimed to histologically evaluate the quality of tissue repair in equine suspensory ligament treated with two cell therapy protocols. All four limbs of six animals were operated simultaneously to remove a fragment in each ligament using a skin biopsy punch. Two days later, intralesional injections were performed using bone marrow mononuclear fraction (BM group), cultivated cells derived from adipose tissue (AT group), saline (positive control group), or no treatment (negative control group), in such way that each horse received all treatments. After sixty days biopsies were performed for histological analysis (H & E, Masson’s trichrome and picrosirius red) and immunohistochemistry analysis (collagen type III). Histological findings (H & E and Masson’s trichrome), birefringence intensity (through picrosirius) and collagen type III expression (through immunohistochemistry) were analyzed. Samples from treated groups had better birefringence intensity (P=0.007) and fiber alignment scores were superior compared to controls, though not statistically significant (P=0.08). Presence of inflammatory cells and intense staining for collagen type III occurred in all groups demonstrating an active healing process. In conclusion, both protocols resulted in improvement of tissue repair indicating their potential to be used as an adjuvant treatment of equine suspensory ligament disorders.
Theorizing feminist research in rural areas of the Punjab, Pakistan

This paper constructed on former work by the authors and on the succeeding literature. It argues that a feminist research provides a powerful tool in assisting us to understand women’s lives, their experiences, their concerns based on gender discrimination.This paper seek for empowerment and liberation of women by conceptualizing and thinking about the findings of feminist researchs which are often applied to promote social changes and social justice for women.The paper begins by outlining why the theorization of term ‘feminist methodology’ is used to tag an ideal approach for conducting feminist research which reveals that the basic premise of feminist theory is that women have been excluded from traditional philosophy. In Pakistan, especially in Punjab, women inferior and subordinated status is in practice, including women’s political deprived affiliation to contest election and cast the vote. Such circumstances tend to make them less empowered.In addition, the relational analyses of liberal feminism and ethnographic structure of the Punjabi society shows a strong correlation that justifies the position of researchers to select a liberal feminist theory as a theoretical framework.The only way to bring changes in such traditional societies are gradual process of reforms. So, strategies of liberal feminists can be more successful as reformists rather than radicals, socialists and Marxists.
Design, simulation and application of a PCL membrane with TiO2 and Ag nanoparticles for a novel geometry fog water harvesting device

Nowadays, the world faces an increasing water crisis problem that will become even more severe in the years to come. Fog-water harvesting represents an economical and efficient alternative to address water scarcity around the world. In this study a report on the development of Polycaprolactone (PCL) composite membranes using TiO2 nanoparticles (NPs), Ag NPs and AgTiO2 NPs is presented, besides the effect of using two different solvents, acetic acid and a mixture of chloroform and N,N-dimethylformamide (DMF), to achieve a hydrophobic material capable of assisting fog-water harvesting applications. The proposal of a novel geometry is presented validated through mechanical and air-flow simulation. Beside the investigation and development of a new manufacture procedure for the application of the composite polymer material on a commercial Rashel mesh to enhance its properties. Surface morphology and roughness were explored by scanning electron and infinity focus microscopy, a more homogeneous morphology was observed for composites elaborated with chloroform-DMF. The degree of crystallinity and structural properties were investigated by differential scanning calorimetry and x-ray diffraction. The interaction between PCL and the dopants NPs was studied by Fourier transform infrared spectroscopy, detecting a shift to lower wavenumbers in the principal polymer modes corroborating a compatibility between the solvents, the NPs and the PCL polymer matrix. The permeability of the membranes was analyzed by water contact angle and the mechanical
OPTH_A_213132 1649..1656

Filomena J Ribeiro Tiago B Ferreira Catarina Relha Carina Esteves Sylvia Gaspar 1Ophthalmology Department, Luz Hospital, Lisbon, Portugal; 2Faculdade De Medicina Da Universidade De Lisboa, Lisbon, Portugal; 3NOVA Medical School, Lisbon, Portugal Purpose: To assess the efficacy of five calculators for toric intraocular lenses (IOL). Methods: Retrospective comparative case series in cataract patients undergoing implantation of trifocal toric IOLs (PhysIOL FineVision POD FT). Inclusion criteria were age-related cataract and a corneal astigmatism between 0.90D and 4.50D. Refractive astigmatism predictability of five different toric calculators or calculation methods were compared. Furthermore, two groups were differentiated according to the type of astigmatism. The mean absolute error and the centroid errors in the predicted residual astigmatism from each calculator were evaluated. Results: Fifty-one eyes of 43 patients were included in the study. For the standard toric calculator using anterior keratometry values only, the centroid prediction error was 0.39D±0.41@166o, which was reduced by the application of the PhysIOL toric calculator that includes the Abulafia-Koch regression formula and adjustment for the effective lens position (0.05D±0.34@167o), and also by the application of the Barrett toric calculator (0.07D±0.28@160o). Regarding the techniques that directly evaluate posterior corneal surface, the Holladay toric calculator, using total corneal power provided by a color-LED topographer, generated better results (0.10D±0.44@156o) than those using Scheimpflug camera data (0.23D±0.56@158o). Similar results were found for both types of astigmatism. Conclusion: The PhysIOL and the Barrett toric calculators taking into account the posterior corneal astigmatism by mathematical models, yielded lower astigmatic prediction errors compared to a standard toric calculator based on anterior keratometry data only. When total corneal power measurements were used, prediction errors were lower with color-LED than with Scheimpflug based topography.
Establishment and characterization of novel human primary endometrial cancer cell line (ZJB-ENC1) and its genomic characteristic

The establishment of human malignant tumor cell lines can provide abundant experimental materials for understanding the biological characteristics of tumors, studying the carcinogenesis, molecular genetics and the mechanism of metastasis and evolution. In this study, a novel cell line designated ZJB-ENC1 has been established from poorly differentiated endometrioid adenocarcinoma. Cytological results showed monolayer-cultured cells were polygonal in shape and a piling-up tendency without contact inhabitation. Immunohistochemistry analysis showed that the cells were negative for ER, PR, c-erbB2, E-CAD, CD117, and OCT3/4, but strongly positive for PTEN and P16. Meanwhile, the tumorigenicity of ZJB-ENC1 was confirmed by subcutaneous transplantation of the cells into a xenograft mouse model. In addition, the results of the whole exome sequencing revealed a unique genomic characteristic of ZJB-ENC1 cells, all common and novel SNPs and InDels were identified. In conclusion, this new stable cell line may promote basic and clinical research on endometrial cancer (EC).
Effect of pH on the properties of ZnO nanostructures prepared by chemical bath deposition method

ZnO powders were prepared by chemical bath deposition method by varying the pH of the precursor. Different amount of ammonia hydroxide were added to change the pH of the solution from 5 to 12 values. The effect of pH of the precursor on the structure, morphology, optical and luminescence properties of ZnO nanostructures were investigated. The X-ray diffraction (XRD) patterns of the ZnO nanostructures correspond to the various planes of a single hexagonal ZnO phase. It was observed that the diffraction peaks increased in intensity with an increase in pH. The estimated average grain sizes calculated using the XRD spectra were found to be in the order of 38 ± 1 nm. It was observed that the estimated average grain sizes increased slightly with an increase in pH. The surface morphology study revealed that the grains were flakes-likes at low pH (< 6) but flower-like for high pH (12). The UV-Vis spectra showed a red shift with an increase in pH. The band gap energy of ZnO was found to decrease but the luminescence intensities increased with an increase in pH values. PL showed that the ultra-violet emission intensity of the nanostructures decreased with an increase in pH but the visible emission increased with an increase in pH.
Effect of Sulfated Polysaccharide from Undaria pinnatifida (SPUP) on Proliferation, Migration, and Apoptosis of Human Prostatic Cancer

Objective. To observe the effect of sulfated polysaccharide from Undaria pinnatifida (SPUP) on proliferation, migration, and apoptosis of human prostatic cancer. Methods. DU145 human prostate cancer cells were cultured in vitro, and the proliferation activity both in the control group and the SPUP treatment groups (25, 50, 100, 200 μg/ml) was measured by CCK-8 assay. The wound healing assay was conducted to detect the cell migration. Cell apoptosis was measured by flow cytometry. The protein and mRNA expressions of matrix metalloproteinase-9 (MMP-9) and apoptosis-related factor Bax were detected by qRT-PCR and Western blot. The expressions of cleaved caspase-3 and cleaved caspase-9 were also determined by Western blot. Results. (1) CCK-8 results showed that the proliferative activity of DU145 cells was significantly decreased with the increase of SPUP treatment concentration (P<0.05) in a dose-dependent manner and that the inhibitory effect of SPUP was most significant at 72 h (P<0.05) as compared with the control group; (2) the migration rate of SPUP-treated cells was significantly decreased (P<0.05) as compared with the control group. And the results of qRT-PCR and Western blot assays showed that SPUP inhibited the expression of MMP-9 in DU145 cells; (3) compared with the control group, the SPUP-treated groups had increased apoptosis of the cells. The expressions of apoptosis-related factors cleaved caspase-3, cleaved caspase-9, and Bax were upregulated (P<0.05), and the mRNA expression of Bax was increased (P<0.05). Conclusion. SPUP showed an antitumor activity in prostatic cancer, and the underlying mechanism may be pertaining to inhibition of migration, proliferation, and induction of apoptosis of cancer cells.
The Diagnostic Value of the Glucose Curve in Individuals with and without Diabetes

Aim: Of this study is to demonstrate the importance of glucose curve test in monitoring pre and post-meal variation in diabetic and normal individuals. Methodology: The individuals subjected to this study mainly grouped in two categories the (DM2 group) and the (Control group), they instructed to came fasting at which blood sample will be collected in EDTA and blank tube then after 30 min. the first post-prandial blood sample collected and then after every 1,2,3,4,5,6,7,8 hours blood sample collected subsequently, then serum separated from each sample (except the EDTA tube) analysed biochemically for glucose and glycated haemoglobin HbA1c (from EDTA tube). Result: We found that, the calculated glucose based on mean glycated haemoglobin HbA1c% results underestimate the real concentrations all over the glucose curve in control group but in DM2 group it underestimate the mean and some actually measured concentration in some points of the curve which adds more burden on the diabetic patient and the responsibility of adjusting the dose and time of administration. Conclusion: from our prospect we recommend the use of blood glucose curve as a monitoring and diagnostic tool generally for glucose metabolism in normal, pre-diabetic, diabetic and uncontrolled diabetic patients before and during therapeutic conditions.
A new species of Baeomorpha (Hymenoptera, Rotoitidae) from mid-Cretaceous Burmese amber

A fossil species from mid-Cretaceous Burmese amber, Baeomorpha liorum Huber, Shih & Ren, sp. nov. (Rotoitidae: Hymenoptera), is described and illustrated. Its relationship to other extinct and extant taxa of Rotoitidae is discussed. The location of the amber deposits in which this species was found is well south of the “Baeomorpha Realm” proposed by previous authors who suggested that the origin and diversification of Rotoitidae occurred in North Laurasia. Based on the Burmese specimens, we suggest instead that the most parsimonious explanation for the past and present distribution of the family is to assume that Rotoitidae is an ancient lineage of Chalcidoidea that was originally widespread in suitable habitats across both Laurasia and Gondwana and later became extinct everywhere except Chile and New Zealand where the two extant genera occur.
Oral health status of disabled children attending special schools of Dhaka city

Background: Children with disabilities and special needs present unique challenges for oral health professionals in the planning and carrying out of dental treatment. oral health care workers need to take cognizance of when preparing treatment plans for children with special needs. Children with disabilities are considered to be a high risk group for dental diseases specially dental caries and periodontal disease. High rates of dental caries, missing teeth, periodontal disease and malocclusion are all indicators of poor oral health of children with disabilities. Objectives:The aim of the study was to assess the oral health status of disabled children in special schools of Dhaka city. Study design: This was a cross sectional study. Study settings and period: The study was conducted in two elementary schools at mirpur area of Dhaka city in the period between of between february 2014 to January 2015. Subjects: 200 disabled children with the age range from 6 to 14 belonging to six different disability groups were examined. Participants were grouped according to their type of disability autistic children, Down syndrome, Cerebralpulsy, mute and deaf, blind and physical disability. Methods: Data was collected by using semi structured quationnaire and checklist.Datawere analyzed by statistical package of social science(SPSS- 20). Results: According to disability the participants autistic 44%, cerebral pulsy 37%, down syndrome 35%, blind 28%, deaf and mute 30% and physical disability 30%. The average number of decayed tooth was found 6.89%. Mean DMFT value was 4.75. Average number of missing tooth was 1.89%. Prevalence of dental caries highest 71% in autistic children then other cetagory of disabilty . Oral hygiene practice of the respondent found very poor.76% children brush teeth in the morning and once in a day.49% respondent use toothpick to clean food from in between teeth.37% of the respondent never visited to dentist. 34.5% respondents had normal gingiva, 49% had mild gingivitis and 16.5% had marked gingivitis. Highest numbers of trauma cases were recorded in visually impaired group. 98.7% children required some form of dental treatment. Conclusion: Oral health status of children with disabilities was poor and it is important to concentrate on a preventive approach and provide proper dental education to parents of disabled childrens. More attention should be paid to the oral hygiene practice of disabled children Update Dent. Coll. j: 2019; 9 (2): 32-35
Determinants of Willingness to Pay for Improved Water Supply in Osogbo Metropolis; Osun State, Nigeria

The s tudy analyzed the willingness to pay for improved water supply service by households in Osogbo metropolis , Osun State. A multis tage ra n d o m sampling technique was employed to select One hundred and forty-two households from areas with public water services and those without connection to public water. Methods of data analyses involved the use of des c rip t iv e s t a tis tics and a binary equation model (logis tic regress ion) for the households sampled. Fifty one (51.35) perc e n t o f households sampled were between 21 and 40 y e a rs wh ile t h e average age was 43 years . The educational level among the households sampled is high with 87.3 p e rc e n t having pos t secondary school education. This perhaps influenced their willingness to pay for improved water supply service s ince education is expected to make the mind more receptive to innovation. On the avera g e , h o u seholds in the s tudy area pay between N250 and N800 per month for water charges depending on level of household consumption. Thirty-eight percent of the households are willing to pay between 1 and 3 perc e n t of their income for alternative water supply service. This has implication on the need for private participatio n in t h e p ro v is ion of water in the s tudy area. The logis tic regress ion es timates , however, revealed that the percentage of inco me that a household is willing to pay for impro v e d wa t e r supply and the willingness to pay for connection charges to the improved source are s tatis tica lly s ig n ificant at P < 0.05 and P < 0.1 respectively. The coefficient of percentage willingness to p a y (W TP) from income is pos itive; this reveals that an increase in income will increase the proportion of income that households would be willin g t o p ay for improved water services .
Original Article

Behrooz Farzanegan 1, Navid Shafigh 2, Jalal Heshmatnia 3, Seyed Mohammadreza Hashemian 3, Mehran Malekshoar 3, Golnaz Afzal 4, Hamidreza Jamaati 3, Mehdi Kazempour-Dizaji 5, Mohammad Fathi 1 Background: Gastric residual volume (GRV) is considered an important parameter for gastric emptying and nutrition tolerance. This volume is measured before any nutrition and has a direct effect on the volume and timing of the next nutrition. The present study aimed to examine the GRV via ultrasound after receiving intravenous ondansetron, metoclopramide, and neostigmine. Materials and Methods: In the present study, 40 patients were included in the study, 10 patients were excluded from the study due to death during treatment, and 30 patients were divided into three groups of 10(10 patients in each group). The first, second, and third groups received 2.5, 10, and 8 mg neostigmine, metoclopramide, and ondansetron every 8 h, respectively. The drugs were infused as a micro set in 100 ml normal saline into patients within 30 min. The patients underwent ultrasound imaging and GRV measurement by an intensive care unit (ICU) subspecialty fellow, who was not aware of the drugs received by the patients, in the 1st h of hospitalization, 6 h after drug injection, and once daily for 4 days. Results: A total of 40 patients entered the study based on inclusion and exclusion criteria. The effect of neostigmine on reducing GRV (Gastric residual volume) in ICU patients was better than those of the other two drugs, which was significant. Conclusion: The results of this study showed that neostigmine has a better and significant effect on reducing GRV in ICU patients, compared to those of ondansetron and metoclopramide.
Research article, J Sport Sci Med

The primary aim of the study was to determine if heart rate variability (HRV), and heart rate recovery (HRR) are related to several subjective indicators of recovery status (muscle soreness, hydration status, sleep quality and quantity as well as precompetition mood states) for different match periods in male, elite, African, singles badminton players. HRV and HRR were measured in twenty-two badminton players before (pre-match), during (in-match), after (post-match) and during rest periods (inmatch rest) of 46 national and international matches. Muscle soreness, hydration status, and sleep quality and quantity were measured on a daily basis whereas mood states were measured just before each match via questionnaires. Prior to each match warm-up, players were fitted with a Fix Polar Heart Rate Transmitter Belt to record heart rate every second during each match and HRR during service breaks and after matches. Kubios HRV software was used for final HRV analyses from the series of R-R-intervals. A strong, significant canonical correlation (Rc = 0.96, p = 0.014) was found between HRV, HRR and subjective indicators of recovery status for the in-match period, but only strong, non-significant relationships were observed for pre-match (Rc = 0.98, p = 0.626) and post-match periods (Rc = 0.98, p = 0.085) and a low non-significant relationship (Rc = 0.69, p = 0.258) for the in-match rest period. Canonical functions accounted for between 47.89% and 96.43% of the total variation between the two canonical variants. Results further revealed that Ln-HFnu, the energy index and vigour were the most prominent variables in the relationship between the autonomic markers of heart rate and recovery-related variables. In conclusion, this study proved that subjective indicators of recovery status influence HRV and HRR measures obtained in a competitive badminton environment and should therefore be incorporated in protocols that evaluate these ANS-related parameters.
THE NEW ZEALAND MEDICAL JOURNAL

Aim To examine the prevalence of weight control practices in a nationwide representative sample of 40–50 year old New Zealand women. Methods In May 2009, a cross-sectional mail survey was conducted among 2500 women randomly selected from nationwide electoral rolls who were each mailed a self-administered questionnaire about their weight control practices, demographics and anthropometry. Results After excluding ineligible recipients, a 66% participation rate was achieved (n=1601). Thirty-nine percent of the sample (n=630) reported trying to control their weight, and among those who were not, 69% (n=674) were attempting to prevent weight gain. The weight control practices most commonly used were cutting down on meal/snack size (42%) and cutting down on fats/sugars (45%). Smoking, exercising in a driven or compulsive way, and fasting were used at least once a week as weight control strategies by 14%, 22% and 5% of women, respectively. Other potentially healthdamaging practices (i.e. laxatives, diuretics, diet pills, vomiting) were employed by 1– 4% of women. Conclusions Most women reported weight control practices that were consistent with public health messages, however the use of smoking and exercising in a driven or compulsive way are of particular concern. The latest Health of New Zealand Adults Survey showed that the prevalence of obesity is particularly high among women in the 45–54 and 55–64 year age groups, reaching 30.8% and 32.1% respectively. 1 Prospective studies overseas have also shown a high risk of weight gain in mid-life, with women aged 42–52 years gaining on average 0.6 kg per year. 2,3 Population surveys in Australia, North America and Europe show that at any time, 6274% of adult women are trying to control their weight. 2,4 In New Zealand, although 59.6% of females aged 10–24 years are trying to control their weight, 5 to our knowledge there is no data regarding the weight control practices of adult women. Overseas studies have shown that both potentially health promoting (e.g. reducing fat intake, increasing physical activity) and potentially health damaging weight control practices (e.g. use of laxatives, smoking, diet pills, diuretic pills) are widely used by women in attempts to achieve an ‘ideal body weight’. 2,6