Miracle-dz/UniFAR-FTUs huggingface dataset:
Exercise-associated hyponatremic encephalopathy and exertional heatstroke in a soldier: High rates of fluid intake during exercise caused rather than prevented a fatal outcome

Abstract Athletes are often advised to drink in order to “fully replace bodyweight losses” in order to prevent exertional heatstroke (EHS) during exercise in the heat. There is little evidence that “dehydration” in the range experienced by athletes adversely affects thermoregulation or is the exclusive cause of EHS. In contrast it is established that excess fluid intake can cause exercise-associated hyponatremia (EAH) sometimes associated with encephalopathy (EAHE). As part of a series of experiments to determine optimal fluid replacement during exercise in the heat, we studied a group of exceptionally well-conditioned and heat-adapted members of the South African National Defence Force. A 20 year old male started a time restricted 50 km route-march in a dry bulb temperature that reached 37.5°C (WBGT of 33.6°C, relative humidity of 85%). Pre-march plasma osmolality, serum [Na+] and total body water measures indicated euhydration. Fluid was available ad libitum and isotonic sports drinks at 5 km intervals. Fluid intake and core body temperature (Tc) were recorded throughout while he was tracked by a global positioning system measuring distance travelled, position and speed. Comparing the total fluid intake of the soldier (12930 mL) to the rest of the participants (mean intake of 9 038 mL) up to 40 km, it is evident that his intake was 3892 mL (approximately 300 mL h-1) more than the mean for group. At approximately 17h14 the soldier was found lying by himself at the side of the route, 2.24 km from the finish point. He passed away the next day in a medical care facility. This tragic event provides the valuable opportunity to present data on the pacing, temperature regulation and fluid consumption of an exceptional athlete during the development of a fatal case of combined EAHE and EHS. Pacing, fluid intake, Tc and environmental condition data are presented for 5km intervals throughout the march. We propose a novel hypothesis on the possible contribution of EAHE to the development of EHS.
Effects of acute exercise on flow-mediated dilatation in healthy humans.

Although the effects of exercise training on vascular function have been well studied, less is known about the effects of acute exercise bouts. This synthesis summarizes and integrates knowledge derived from papers relating acute impacts of exercise on artery function, specifically endothelial function assessed by flow-mediated dilatation (FMD). We propose that an immediate decrease in FMD ("nadir") occurs soon after exercise cessation and that this is followed by a (supra)normalization response. The magnitude of the nadir and (supra)normalization and duration of this biphasic pattern of response appears to be influenced by numerous factors, including the nature of the exercise stimulus (e.g., type, duration, intensity), the subject population (e.g., trained vs. untrained), and various methodological factors. The impact of these factors on the biphasic pattern are most likely mediated through stimuli that underpin altered FMD postexercise, including shear and oxidative stress, changes in arterial diameter, and antioxidant status. We propose that a combination of these stimuli act synergistically to balance the vasomotor responses postexercise. Finally, we discuss the potential (clinical) relevance of the biphasic response after acute exercise, as the immediate nadir may represent an essential response for subsequent training-induced adaptations but may also represent a transient period of increased cardiovascular risk leading to the "exercise paradox. "
Interferon‐γ is up‐regulated in the hippocampus in response to intermittent fasting and protects hippocampal neurons against excitotoxicity

Dietary restriction (DR) increases the life span of many different organisms, and recent findings have demonstrated neuroprotective effects of DR in rodent and nonhuman primate models of neurodegenerative disorders. The neuroprotective mechanism of action of DR is unknown, but it may result from a mild cellular stress response involving increased production of neurotrophic factors. Because several different cytokines are known to be up‐regulated in brain cells in response to stress, we determined whether DR affected cytokine expression in the rat brain. Levels of expression of interferon‐γ (IFN‐γ) and its receptor were significantly increased in the hippocampus of rats that had been maintained on an intermittent fasting DR regimen compared with rats on the ad libitum control diet. Pretreatment of embryonic rat hippocampal cell cultures with IFN‐γ protected neurons against glutamate‐induced death. IFN‐γ‐mediated neuroprotection was associated with an enhanced recovery of intracellular Ca2+ concentrations following exposure to glutamate. Our data show that intermittent fasting DR stimulates IFN‐γ‐mediated neuroprotective signaling in the hippocampus, suggesting a role for this cytokine in the previously reported ability of DR to protect neurons in animal models of severe epileptic seizures, stroke, and neurodegenerative disorders. © 2006 Wiley‐Liss, Inc.
Chronic Maternal Fluoxetine Infusion in Pregnant Sheep: Effects on the Maternal and Fetal Hypothalamic-Pituitary-Adrenal Axes

Depression during pregnancy is frequently treated with the selective serotonin reuptake inhibitor, fluoxetine (FX). FX increases serotonergic neurotransmission and serotonin plays a role in the regulation of the hypothalamic-pituitary-adrenal (HPA) axis. We have therefore investigated the effect of chronic administration of FX to the pregnant ewe on the maternal and fetal HPA axes. Nineteen late-gestation sheep were surgically prepared for chronic study of the fetus. FX (n = 7, 98.5 μg/kg/d) or sterile water (control, n = 8) was administered to the ewe for 8 d by constant rate i.v. infusion with an initial FX bolus dose of 70 mg. Maternal and fetal plasma ACTH and cortisol concentrations were determined at 0700 h each day. Maternal plasma ACTH concentrations fell on infusion d 2, but no changes were observed in maternal plasma cortisol concentrations. Fetal plasma ACTH concentrations increased on infusion d 7, and fetal plasma cortisol concentrations increased on infusion d 6, 7, and 8 in the FX group. In addition, the regression coefficient for the relationship between fetal ACTH and cortisol levels was significantly greater in the FX group compared with the control group. Thus, maternal FX treatment increased fetal plasma cortisol concentration. These results are of particular interest in the context that exposure of the fetus to excess glucocorticoids at critical windows during development has been shown to increase the risk of poor health outcomes in later life.
Pathologic and Immunocytochemical Studies of Morbillivirus Infection in Striped Dolphins (Stenella coeruleoalba)

Hundreds of striped dolphins (Stenella coeruleoalba) died along the Spanish Mediterranean coast during the second half of 1990. We necropsied 58 dolphins. Partial collapse of the lungs with patchy atelectasis, subcutaneous edema, icterus, and stomatitis were the most prominent gross morphologic changes. Histologically, a bronchiolo-interstitial pneumonia was the most frequent lesion (72% of the animals). It was characterized by hyperplasia of alveolar epithelial type II cells and formation of multinucleate syncytia in alveolar and bronchiolar lumina. Other prominent lesions were encephalitis (69%), lymphoid depletion, and formation of multinucleate syncytia in the cortex of lymph nodes. The distribution of morbillivirus antigen was investigated in 23 wellpreserved dolphins using a monoclonal antibody against the hemagglutinin glycoprotein of phocine distemper virus. Positive immunostaining was found in brain (77%), in lung (70%), and in mesenteric (61%), mediastinal (47%), and prescapular (45%) lymph nodes. Phocine distemper virus antigen was demonstrated less frequently in trachea, stomach, biliary epithelium, intestine, kidney, and mammary gland. Necrotizing-hemorrhagic pneumonia and encephalitis due to Aspergillus fumigatus were seen in three dolphins, whereas two animals had lesions of toxoplasmosis. Changes in our dolphins were similar to those caused by distemper in seals and porpoises. The origin of the dolphin virus and the relationships among dolphin, seal, and porpoise morbilliviruses are unknown.
Age- and sex-dependent disparity in physical fitness between obese and normal weight children and adolescents.

AIM Aim of the present study was to determine whether the association between obesity and physical fitness in children and adolescents is age- and sex-specific. METHODS A total of 4519 children and adolescents aged 4-17 years grouped by age (4-5, 6-10, 11-13 and 14-17 years), sex and BMI (normal-weight: BMI≤90th percentile; overweight: BMI>90th percentile; adipose: BMI>97th percentile) participated in this large-scale representative cross-sectional study. Endurance, strength, flexibility and fine and gross motor coordination was measured using the following physical fitness tests: PWC170, push-ups test, standing long-jumps, side-to-side jumps, one-minute single leg stance on the dominant leg, balancing backwards on three beams of different width, lowest point reached by the fingertips while standing on a box with legs extended, reaction to colour changes of traffic light, tracing lines without touching the rim and sorting 25 pens. RESULTS Overweight and obese boys and girls had lower physical fitness values describing endurance, strength and gross motor coordination than normal-weight boys and girls, respectively (P<0.001 for all; boys: -12% and -19%, respectively; girls: -9% and -19%, respectively). Differences in physical fitness between weight groups were greater in older groups (P<0.001). Fine motor skills and flexibility values did not differ between weight groups. CONCLUSION The disparity in physical fitness, in particular in endurance, strength and gross motor coordination, between obese and normalweight is greater in adolescents than in children. Physical fitness programs are warranted for all overweight and obese children and adolescents but especially for overweight and obese adolescents.
Recurrent generalized seizures, visual loss, and palinopsia as phenotypic features of neuronal ceroid lipofuscinosis due to progranulin gene mutation

We detail the phenotype of a novel form of neuronal ceroid lipofuscinosis due to a homozygous progranulin gene mutation (c.813_816del; CLN11 MIM #614706). The symptoms appeared in two young adult siblings, and included progressive retinopathy, recurrent generalized seizures, moderate ataxia, and subtle cognitive dysfunction. Long‐lasting episodes of palinopsia were a recurring symptom and associated with polyphasic visual‐evoked potential waveform that suggested hyperexcitability of the occipital cortex. Electroencephalography showed rare spike‐wave paroxysms, and magnetic resonance imaging revealed selective cerebellar atrophy. Skin biopsy revealed fingerprint storage and the absence of progranulin protein. Electron microscopy of peripheral blood leukocytes showed fingerprint profiles in 1/100 lymphocytes. These findings define a novel phenotype and provide clues for better understanding of progranulin function.
Five-year quality of life assessment after carbon ion radiotherapy for prostate cancer

Abstract The aim of this study was to prospectively assess 5-year health-related quality of life (HRQOL) of patients treated with carbon ion radiotherapy (C-ion RT) for clinically localized prostate cancer. A total of 417 patients received carbon ion radiotherapy at a total dose of 63–66 Gray-equivalents (GyE) in 20 fractions over 5 weeks, and neoadjuvant and adjuvant androgen deprivation therapy (ADT) were administered for intermediate and high-risk patients. A HRQOL assessment was performed at five time points (immediately before the initiation of C-ion RT, immediately after, and at 12, 36 and 60 months after completion of C-ion RT) using Functional Assessment of Cancer Therapy (FACT) questionnaires. FACT-G and FACT-P scores were significantly decreased; however, the absolute change after 60 months was minimal. The transient decreases in the Trial Outcome Index (TOI) score returned to their baseline levels. Use of ADT, presence of adverse events, and biochemical failure were related to lower scores. Scores of subdomains of FACT instruments indicated characteristic changes. The pattern of HRQOL change after C-ion RT was similar to that of other modalities. Further controlled studies focusing on a HRQOL in patients with prostate cancer are warranted.
An Exploratory Study of HIV Risk Behaviors and Testing among Male Sex Workers in Beirut, Lebanon

Male sex workers (MSW) are a particularly high-risk subset of men who have sex with men in Lebanon and report higher numbers of sex partners and lower rates of condom use. The purpose was to explore the factors influencing sexual risk behaviors and HIV testing among MSW. Qualitative interviews were conducted with 16 MSW living in Beirut and working in bathhouses (hammam) or as escorts; content analysis identified emergent themes. Escorts reported more consistent condom use with clients and HIV testing than hammam MSW, with influential factors including HIV risk knowledge and perceived risk susceptibility, job security, and internalized stigma and related feelings of self-worth and fatalism regarding health and HIV risk. In contrast, both groups of MSW typically opted not to condoms with nonclient sex partners, in an effort to differentiate sex for work versus pleasure. The uptake of HIV testing was limited by concerns about the confidentiality of the test results and fear of repercussions of a positive test result for their health and employment. The respondents described an insular existence within the sex work culture, in part to limit exposure to stigma, which has implications for access to support as well as the influence of peer norms regarding sexual risk behavior and health seeking behaviors such as HIV testing. Further research is needed to tailor prevention and HIV testing efforts to reflect the distinct sexual health “cultures” that distinguish these two populations of MSW in Lebanon.
Almotriptan for menstrually related migraine

Introduction: Approximately 50% of migrainous women associate their headache temporally to menses. Menstrually related migraine (MRM) is a disabling form of migraine characterized by attacks that are generally longer, more severe and less drug-responsive than nonmenstrual ones. Since MRM may be difficult to treat, it is important to find an appropriate treatment option for women suffering from this condition. Areas covered: This paper provides an overview of the clinical features of MRM, with special attention on the use of almotriptan for its treatment. Four studies on almotriptan in the treatment of MRM are present in the medical literature. Two report post hoc analyses of data derived from larger studies on the use of almotriptan for migraine treatment. One reports the results from a study specifically dedicated to MRM and one illustrates a subanalysis on the accompanying symptomatology. Expert opinion: Evidence demonstrates that almotriptan is a molecule with a high efficacy in the treatment of MRM and with an excellent tolerability profile when compared with other triptans. Moreover, it shows a proven ability to control migraine-associated symptoms. All these qualities play a decidedly positive role in making almotriptan a product of choice for the treatment of MRM.
Superparamagnetic iron oxide-mediated hepatic signal intensity change in patients with and without cirrhosis: pulse sequence effects and Kupffer cell function.

PURPOSE To analyze superparamagnetic iron oxide (SPIO)-mediated hepatic signal intensity change in cirrhotic and noncirrhotic liver and to investigate the relationship between pulse sequence effects in SPIO-enhanced magnetic resonance (MR) imaging for hepatic cirrhosis. MATERIALS AND METHODS Twelve patients with and 12 patients without cirrhosis underwent T2-weighted fast spin-echo, T2*-weighted gradient-echo (GRE), and T1-weighted GRE MR imaging before and twice (early and late phase) after SPIO administration. To assess the effect of SPIO, postcontrast relative signal-to-noise ratio (SNR) changes were statistically analyzed with repeated measurements analysis of variance for each pulse sequence. RESULTS No interaction was shown between groups and data time points for any pulse sequence. There was no significant difference in mean hepatic relative SNR change on T2-weighted fast spin-echo images between the cirrhotic group and noncirrhotic group (-38.6% and -40.7%, early phase; -42.2% and -49.6%, late phase, respectively). For GRE images, statistically significant differences in mean hepatic relative SNR change were found between the cirrhotic group and noncirrhotic group (-14.2% and -44.5%, early phase; -28.5% and -56.4%, late phase on T2*-weighted GRE images (P <.001); 31.8% and 12.9%, early phase; 23.8% and 2.2%, late phase on T1-weighted GRE images (P <.05), respectively. CONCLUSION Decreased overall phagocytic activity in cirrhotic liver is more likely due to Kupffer cell dysfunction than to Kupffer cell depletion, since magnetic susceptibility effects on T2*-weighted GRE images depend on intracellular SPIO cluster size.
The neural substrate for working memory of tactile surface texture

Fine surface texture is best discriminated by touch, in contrast to macro geometric features like shape. We used functional magnetic resonance imaging and a delayed match‐to‐sample task to investigate the neural substrate for working memory of tactile surface texture. Blindfolded right‐handed males encoded the texture or location of up to four sandpaper stimuli using the dominant or non‐dominant hand. They maintained the information for 10–12 s and then answered whether a probe stimulus matched the memory array. Analyses of variance with the factors Hand, Task, and Load were performed on the estimated percent signal change for the encoding and delay phase. During encoding, contralateral effects of Hand were found in sensorimotor regions, whereas Load effects were observed in bilateral postcentral sulcus (BA2), secondary somatosensory cortex (S2), pre‐SMA, dorsolateral prefrontal cortex (dlPFC), and superior parietal lobule (SPL). During encoding and delay, Task effects (texture > location) were found in central sulcus, S2, pre‐SMA, dlPFC, and SPL. The Task and Load effects found in hand‐ and modality‐specific regions BA2 and S2 indicate involvement of these regions in the tactile encoding and maintenance of fine surface textures. Similar effects in hand‐ and modality‐unspecific areas dlPFC, pre‐SMA and SPL suggest that these regions contribute to the cognitive monitoring required to encode and maintain multiple items. Our findings stress both the particular importance of S2 for the encoding and maintenance of tactile surface texture, as well as the supramodal nature of parieto‐frontal networks involved in cognitive control. Hum Brain Mapp, 2013. © 2012 Wiley Periodicals, Inc.
Gaze-Adaptive Lenses for Feature-Rich Information Spaces

The inspection of feature-rich information spaces often requires supportive tools that reduce visual clutter without sacrificing details. One common approach is to use focus+context lenses that provide multiple views of the data. While these lenses present local details together with global context, they require additional manual interaction. In this paper, we discuss the design space for gaze-adaptive lenses and present an approach that automatically displays additional details with respect to visual focus. We developed a prototype for a map application capable of displaying names and star-ratings of different restaurants. In a pilot study, we compared the gaze-adaptive lens to a mouse-only system in terms of efficiency, effectiveness, and usability. Our results revealed that participants were faster in locating the restaurants and more accurate in a map drawing task when using the gaze-adaptive lens. We discuss these results in relation to observed search strategies and inspected map areas.
Valproic acid sensitizes human glioma cells to gefitinib‐induced autophagy

Autophagy and apoptosis represent important cellular processes involved in cancer cell killing mechanisms. Epidermal growth factor receptor inhibitor gefitinib and valproic acid have been implicated in the treatment of malignancies including glioma involving autophagic and apoptotic mechanisms. Therefore, it is interesting to investigate whether a combination of gefitinib and valproic acid shows better cancer cell killing effect on human glioma cells. We found that a nontoxic concentration of valproic acid sensitized U87 and T98G glioma cells to gefitinib cytotoxicity by inhibiting cell growth and long‐term clonogenic survival. The augmented consequences were accompanied by the formation of autophagic vacuoles, conversion of microtubule‐associated protein‐1 light chain 3‐II (LC3‐II), and degradation of p62. Autophagy inhibitor 3‐methyladenosine and chloroquine and genetic silencing of LC3 but not broad‐spectrum caspase inhibitor attenuated gefitinib/valproic acid‐induced growth inhibition. Gefitinib/valproic acid‐induced autophagy was accompanied by the activation of liver kinase‐B1 (LKB1)/AMP‐activated protein kinase (AMPK)/ULK1. Silencing of AMPK and ULK1 suppressed gefitinib/valproic acid‐induced autophagy and growth inhibition. Mechanistic studies showed that gefitinib/valproic acid increased intracellular reactive oxygen species generation and N‐acetyl cysteine attenuated gefitinib/valproic acid‐caused autophagy and growth inhibition. In addition to demonstrating the autophagic mechanisms of gefitinib/valproic acid, the results of this study further suggest that intracellular oxidative stress and the LKB1/AMPK signaling might be a potential target for the development of therapeutic strategy against glioma. © 2015 IUBMB Life, 67(11):869–879, 2015
Plastids Are Major Regulators of Light Signaling in Arabidopsis1[W][OA]

We previously provided evidence that plastid signaling regulates the downstream components of a light signaling network and that this signal integration coordinates chloroplast biogenesis with both the light environment and development by regulating gene expression. We tested these ideas by analyzing light- and plastid-regulated transcriptomes in Arabidopsis (Arabidopsis thaliana). We found that the enrichment of Gene Ontology terms in these transcriptomes is consistent with the integration of light and plastid signaling (1) down-regulating photosynthesis and inducing both repair and stress tolerance in dysfunctional chloroplasts and (2) helping coordinate processes such as growth, the circadian rhythm, and stress responses with the degree of chloroplast function. We then tested whether factors that contribute to this signal integration are also regulated by light and plastid signals by characterizing T-DNA insertion alleles of genes that are regulated by light and plastid signaling and that encode proteins that are annotated as contributing to signaling, transcription, or no known function. We found that a high proportion of these mutant alleles induce chloroplast biogenesis during deetiolation. We quantified the expression of four photosynthesis-related genes in seven of these enhanced deetiolation (end) mutants and found that photosynthesis-related gene expression is attenuated. This attenuation is particularly striking for Photosystem II subunit S expression. We conclude that the integration of light and plastid signaling regulates a number of END genes that help optimize chloroplast function and that at least some END genes affect photosynthesis-related gene expression.