Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Linoleic Acids on the Release of β-Carotene from Carotenoid-Producing Saccharomyces cerevisiae into Sunflower Oil

In situ extraction is important for highly productive and cost-efficient processes in industrial biotechnology, but it is difficult to establish for intracellularly accumulating carotenoids like β-carotene. In this study, the organic solvent used in aqueous-organic two-phase media exerted a strong effect on the release of β-carotene from recombinant yeast cells. The carotenoid-synthesizing Saccharomyces cerevisiae strain YB/I/E was cultivated in two-liquid-phase media with 20% dodecane or 20% sunflower oil. Up to 0.6 µg/ml β-carotene was released into sunflower oil, but less than 0.1 µg/ml into dodecane, although biocompatibility and solubility of β-carotene is appropriate for both solvents. Addition of linoleic acid, the main component of sunflower oil, to the dodecane phase increased the amount of β-carotene released, indicating that linoleic acid is the component responsible for the β-carotene release into sunflower oil. These findings demonstrate that the effect of the organic solvent should be taken into consideration for further research on in situ extraction of carotenoids.
Peritoneal Dialysis Catheter Increases Leukocyte Recruitment in the Mouse Parietal Peritoneum Microcirculation and Causes Fibrosis

♦ Background: The objective of this study was to examine the effects of a conventional dialysis solution and peritoneal catheter on leukocyte-endothelial cell interactions in the microcirculation of the parietal peritoneum in a subacute peritoneal dialysis (PD) mouse model. ♦ Methods: An intraperitoneal (IP) catheter with a subcutaneous injection port was implanted into mice and, after a 2-week healing period, the animals were injected daily for 6 weeks with a 2.5% dextrose solution. Intravital microscopy (IVM) of the parietal peritoneum microcirculation was performed 4 hours after the last injection of the dialysis solution. Leukocyte-endothelial cell interactions were quantified and compared with catheterized controls without dialysis treatment and naïve mice. ♦ Results: The number of rolling and extravascular leukocytes along with peritoneal fibrosis and neovascularization were significantly increased in the catheterized animals compared with naïve mice but did not significantly differ between the 2 groups of catheterized animals with sham injections or dialysis solution treatment. ♦ Conclusion: The peritoneal catheter implant increased leukocyte rolling and extravasation, peritoneal fibrosis and vascularization in the parietal peritoneum independently from the dialysis solution treatment.
Iron Exposure and the Cellular Mechanisms Linked to Neuron Degeneration in Adult Mice

Although the causal relationship between Alzheimer’s disease (AD) and iron overload remains unclear, iron dyshomeostasis or improper transport mechanisms are speculated to lead to the accumulation of this neurotoxic metal in the hippocampal formation and other cerebral areas related to neurodegenerative diseases, resulting in the formation of reactive oxygen species (ROS) and, ultimately, cell death. In this study, exposure to high dietary iron (HDI) revealed no significant difference in the number of iron-positive cells and iron content in the cortex and hippocampal region between wild-type (WT) and APP/PS1 mice; however, compared with the control mice, the HDI-treated mice exhibited upregulated divalent metal transporter 1 (DMT1) and ferroportin (Fpn) expression, and downregulated transferrin receptor (TFR) expression. Importantly, we confirmed that there were significantly fewer NeuN-positive neurons in both APP/PS1 and WT mice given HDI, than in the respective controls. Moreover, this iron-induced neuron loss may involve increased ROS and oxidative mitochondria dysfunction, decreased DNA repair, and exacerbated apoptosis and autophagy. Although HDI administration might trigger protective antioxidant, anti-apoptosis, and autophagy signaling, especially in pathological conditions, these data clearly indicate that chronic iron exposure results in neuronal loss due to apoptosis, autophagy, and ferroptosis, hence increasing the risk for developing AD.
A Call to Standardize Definitions, Data Collection, and Outcome Assessment to Improve Care in Alcohol‐Related Liver Disease

Alcohol‐related liver disease (ALD) is highly prevalent and appears to be increasingly reported with worsening mortality; thus, optimizing care in this patient population is imperative. This will require a multidisciplinary, multifaceted approach that includes recognizing alcohol use disorder (AUD) and existing treatments for AUD. We must also acknowledge the full spectrum of ALD clinically and histologically. For example, our current clinical definitions of alcohol‐related hepatitis (AH) do not address that >95% of severe AH occurs in the setting of cirrhosis with <60% of liver explants having hepatitis. Given that the majority of ALD studies rely on clinical diagnosis and lack pathologic confirmation, prior data on the efficacy of medical treatment or use of transplantation are likely limited by intertrial and intratrial heterogeneity. Added limitations of the current field include the inconsistent reporting of relapse with the use of varying definitions and unreliable assessments. Moreover, studies fail to consistently capture the data variables that likely influence the main outcomes of interest in this population—mortality and relapse—and a global effort to create a standardized data collection tool moving forward could help effectively and efficiently aid in the advancement of this field. Conclusion: To optimize patient care and make best use of a limited resource, a systematic change in the approach to research in this population must be undertaken that creates consistent definitions for use in future research to generate reliable and reproducible results. With this in mind, we concisely reviewed the literature to summarize the current state of treating and managing ALD, the heterogeneity in definitions, and the significant opportunities for clinical and research improvement.
Gene and protein expression profiles of JAK-STAT signalling pathway in the developing brain of the Ts1Cje down syndrome mouse model

Abstract Aims: The JAK-STAT signalling pathway is one of the key regulators of pro-gliogenesis process during brain development. Down syndrome (DS) individuals, as well as DS mouse models, exhibit an increased number of astrocytes, suggesting an imbalance of neurogenic-to-gliogenic shift attributed to dysregulated JAK-STAT signalling pathway. The gene and protein expression profiles of JAK-STAT pathway members have not been characterised in the DS models. Therefore, we aimed to profile the expression of Jak1, Jak2, Stat1, Stat3 and Stat6 at different stages of brain development in the Ts1Cje mouse model of DS. Methods: Whole brain samples from Ts1Cje and wild-type mice at embryonic day (E)10.5, E15, postnatal day (P)1.5; and embryonic cortex-derived neurospheres were collected for gene and protein expression analysis. Gene expression profiles of three brain regions (cerebral cortex, cerebellum and hippocampus) from Ts1Cje and wild-type mice across four time-points (P1.5, P15, P30 and P84) were also analysed. Results: In the developing mouse brain, none of the Jak/Stat genes were differentially expressed in the Ts1Cje model compared to wild-type mice. However, Western blot analyses indicated that phosphorylated (p)-Jak2, p-Stat3 and p-Stat6 were downregulated in the Ts1Cje model. During the postnatal brain development, Jak/Stat genes showed complex expression patterns, as most of the members were downregulated at different selected time-points. Notably, embryonic cortex-derived neurospheres from Ts1Cje mouse brain expressed lower Stat3 and Stat6 protein compared to the wild-type group. Conclusion: The comprehensive expression profiling of Jak/Stat candidates provides insights on the potential role of the JAK-STAT signalling pathway during abnormal development of the Ts1Cje mouse brains.
The Functional Independence Measure 18-item version can be reported as a unidimensional interval-scaled metric: Internal construct validity revisited.

OBJECTIVE Since the 1990s the Functional Independence Measure (FIM™) was believed to measure 2 different constructs, represented by its motor and cognitive subscales. The practice of reporting FIM™ total scores, together with recent developments in the understanding of the influence of locally dependent items on fit to the Rasch model, raises the question of whether the FIM™ 18-item version can be reported as a unidimensional interval-scaled metric. DESIGN Rasch analysis of the FIM™ using testlet approaches to accommodate local response dependency. PATIENTS A calibration sample containing 946 cases of data from 11,103 patients undergoing neurological or musculoskeletal rehabilitation in Switzerland in 2016. RESULTS Baseline analysis and the traditional testlet approach showed no fit with the Rasch model. When items were grouped into 2 testlets, fit to the Rasch model was achieved, indicating unidimensionality across all 18 items. A transformation table to convert FIM™ raw ordinal scores to the corresponding Rasch interval scaled values was created. CONCLUSION This study provides evidence that FIM™ total scores represent a unidimensional set of items, supporting their use in clinical practice and outcome reporting when applying the respective transformation table. This provides a basis for standardized reporting of functioning.
MicroRNA‐449b‐5p targets HMGB1 to attenuate hepatocyte injury in liver ischemia and reperfusion

MicroRNAs (miRNAs) participate in the pathological process of liver ischemia/reperfusion (I/R) injury. MiR‐449b‐5p is the target miRNA of high mobility group box 1 (HMGB1). Its role and molecular mechanism in liver I/R injury remain unidentified. In this study, we found a protective effect of miR‐449b‐5p against hepatic I/R injury. HMGB1 expression significantly increased, whereas miR‐449b‐5p dramatically decreased in patients after liver transplant and in L02 cells exposed to hypoxia/reoxygenation (H/R). A dual‐luciferase reporter assay confirmed the direct interaction between miR‐449b‐5p and the 3′ untranslated region of HMGB1 messenger RNA. We also found that overexpression of miR‐449b‐5p significantly promoted cell viability and inhibited cell apoptosis of L02 cells exposed to H/R. Moreover, miR‐449b‐5p repressed HMGB1 protein expression and nuclear factor‐κB (NF‐κB) pathway activation in these L02 cells. In an in vivo rat model of hepatic I/R injury, overexpression of miR‐449b‐5p significantly decreased alanine aminotransferase and aspartate aminotransferase and inhibited the HMGB1/NF‐κB pathway. Our study thus suggests that miR‐449b‐5p alleviated hepatic I/R injury by targeting HMGB1 and deactivating the NF‐κB pathway, which may provide a novel and promising therapeutic target for hepatic I/R injury.
Estimating Ground-Level Concentrations of Multiple Air Pollutants and Their Health Impacts in the Huaihe River Basin in China

Air pollutants existing in the environment may have negative impacts on human health depending on their toxicity and concentrations. Remote sensing data enable researchers to map concentrations of various air pollutants over vast areas. By combining ground-level concentrations with population data, the spatial distribution of health impacts attributed to air pollutants can be acquired. This study took five highly populated and severely polluted provinces along the Huaihe River, China, as the research area. The ground-level concentrations of four major air pollutants including nitrogen dioxide (NO2), sulfate dioxide (SO2), particulate matters with diameter equal or less than 10 (PM10) or 2.5 micron (PM2.5) were estimated based on relevant remote sensing data using the geographically weighted regression (GWR) model. The health impacts of these pollutants were then assessed with the aid of co-located gridded population data. The results show that the annual average concentrations of ground-level NO2, SO2, PM10, and PM2.5 in 2016 were 31 µg/m3, 26 µg/m3, 100 µg/m3, and 59 µg/m3, respectively. In terms of the health impacts attributable to NO2, SO2, PM10, and PM2.5, there were 546, 1788, 10,595, and 8364 respiratory deaths, and 1221, 9666, 46,954, and 39,524 cardiovascular deaths, respectively. Northern Henan, west-central Shandong, southern Jiangsu, and Wuhan City in Hubei are prone to large health risks. Meanwhile, air pollutants have an overall greater impact on cardiovascular disease than respiratory disease, which is primarily attributable to the inhalable particle matters. Our findings provide a good reference to local decision makers for the implementation of further emission control strategies and possible health impacts assessment.
Multilevel Control Over Sexuality of Unmarried Adolescent Girls and Related Violence: A Qualitative Exploration in Slums of Dhaka, Bangladesh

High concerns about sexuality of unmarried adolescent girls (UAGs) in patriarchal societies and perceived links between sexuality and honor are likely to trigger controlling behavior and underlie a range of violence perpetrated against them. These concerns are heightened in the urban slums of Bangladesh, where economic and social changes challenge traditional gender roles and behaviors. Little is, however, known about control of and violence against UAGs perpetrated by families, romantic partners, and community. Using 12 key informant interviews, 15 focus group discussions, and 27 in-depth interviews carried out between June and November 2011 in Dhaka slums, this study explores the sociocultural expectations around sexuality of UAGs, how they interact with ground reality, control, resultant conflicts, violence against UAGs, and the ways UAGs grapple with controlling behavior and violence. The findings show that perceptions, attitudes, and expectations about the behavior of these girls remain patriarchal despite changes in economic and social circumstances reshaping gender roles and practices. Patriarchy finds new ways of controlling the girls by imposing dress codes, setting new boundaries for mobility and interaction with the opposite sex, and inflicting different forms of violence at different levels of the society. Some girls attempt to avoid violence by conforming to the new rules, while many negotiate the new boundaries with the family, romantic partner, and community. UAGs sometimes make compromises in one domain or level, while they resist control and violence in another. The findings lend support to the patriarchal bargain theory. Promotion of nonpatriarchal norms is expected to reduce vulnerability of these girls to violence.
Genetic interactions support an inhibitory relationship between bone morphogenetic protein 2 and netrin 1 during semicircular canal formation

ABSTRACT The semicircular canals of the mammalian inner ear are derived from epithelial pouches in which epithelial cells in the central region of each pouch undergo resorption, leaving behind the region at the rim to form a tube-shaped canal. Lack of proliferation at the rim and/or over-clearing of epithelial cells in the center of the pouch can obliterate canal formation. Otic-specific knockout of bone morphogenetic protein 2 (Bmp2) results in absence of all three semicircular canals; however, the common crus and ampullae housing the sensory tissue (crista) are intact. The lack of Bmp2 causes Ntn1 (which encodes netrin 1), which is required for canal resorption, to be ectopically expressed at the canal rim. Ectopic Ntn1 results in reduction of Dlx5 and Lmo4, which are required for rim formation. These phenotypes can be partially rescued by removing one allele of Ntn1 in the Bmp2 mutants, indicating that Bmp2 normally negatively regulates Ntn1 for canal formation. Additionally, non-resorption of the canal pouch in Ntn1−/− mutants is partially rescued by removing one allele of Bmp2. Thus, reciprocal inhibition between Bmp2 and netrin 1 is involved in canal formation of the vestibule. Summary: Bmp2-conditional mutant analyses support the hypothesis that presumptive crista induces canal genesis zone in the canal pouch to express Bmp2, which promotes canal formation by restricting Ntn1 expression to the resorption domain.
Regeneration of Solanum tuberosum Plants from Protoplasts Induces Widespread Genome Instability[OPEN]

Genome sequencing in potato plants regenerated from protoplasts reveals widespread changes in chromosome number and structure. Nontransgenic genome editing in regenerable protoplasts, plant cells free of their cell wall, could revolutionize crop improvement because it reduces regulatory and technical complexity. However, plant tissue culture is known to engender frequent unwanted variation, termed somaclonal variation. To evaluate the contribution of large-scale genome instability to this phenomenon, we analyzed potatoes (Solanum tuberosum) regenerated from either protoplasts or stem explants for copy number changes by comparison of Illumina read depth. Whereas a control set of eight plants that had been propagated by cuttings displayed no changes, all 15 protoplast regenerants tested were affected by aneuploidy or structural chromosomal changes. Certain chromosomes displayed segmental deletions and duplications ranging from one to many. Resampling different leaves of the same plant found differences in three regenerants, indicating frequent persistence of instability. By comparison, 33 regenerants from stem explants used for Agrobacterium-mediated transformation displayed less frequent but still considerable (18%) large-scale copy number changes. Repetition of certain instability patterns suggested greater susceptibility in specific genomic sites. These results indicate that tissue culture, depending on the protocol used, can induce genomic instability resulting in large-scale changes likely to compromise final plant phenotype.
Using the Footfall Sound of Dairy Cows for Detecting Claw Lesions

Simple Summary Claw lesions affect the health and well-being of dairy cows and are the third most frequent reason for culling. The most common method to identify cows affected by claw lesions is the assessment of locomotion. However, this requires apart from a trained observer a lot of time. And time is an important limiting factor especially with increasing herd sizes. Thus, regarding the detection of claw lesions, a sensor-assisted, automated system to be implemented on farms should be developed. This system analyzes the footfall sound of cows to distinguish lame from non-lame animals. Using a runway with sensors for recording the footfall sound, we found that cows with non-infectious diseases such as sole ulcers showed a less forceful gait pattern than healthy ones. It is known that particular cows with non-infectious diseases have a greater sensitivity to pain. Therefore the established system is capable of selecting cows that take more cautious steps. This character of abnormal gait pattern is in turn a sign of lameness. Therefore, the automated system as developed in this study is a promising tool for detecting lameness in dairy cows on farms. Abstract An important factor for animal welfare in cattle farming is the detection of lameness. The presented study is part of a project aiming to develop a system that is capable of an automated diagnosis of claw lesions by analyzing the footfall sound. Data were generated from cows walking along a measurement zone where piezoelectric sensors recorded their footfall sounds. Locomotion of the animals was scored and they were graded according to a three-scale scoring system (LS1 = non-lame; LS2 = uneven gait; LS3 = lame). Subsequently, the cows were examined by a hoof trimmer. The walking speed across the test track was significantly higher in cows with LS1 compared to those with LS2 and LS3 and thus, they were showing a smoother gait pattern. The standard deviation of volume (SDV) in the recorded footfall sound signal was considered as a factor for the force of a cow’s footsteps. Cows with non-infectious claw lesions showed lower SDV than healthy cows and those with infectious claw diseases. This outcome confirmed the hypothesis that the evaluated cows affected by non-infectious claw lesions have a greater sensitivity to pain and demonstrate a less forceful gait pattern. These first results clearly show the potential of using footfall sound analysis for detecting claw lesions.
Populus euphratica JRL Mediates ABA Response, Ionic and ROS Homeostasis in Arabidopsis under Salt Stress

Sodium chloride (NaCl) induced expression of a jacalin-related mannose-binding lectin (JRL) gene in leaves, roots, and callus cultures of Populus euphratica (salt-resistant poplar). To explore the mechanism of the PeJRL in salinity tolerance, the full length of PeJRL was cloned from P. euphratica and was transformed into Arabidopsis. PeJRL was localized to the cytoplasm in mesophyll cells. Overexpression of PeJRL in Arabidopsis significantly improved the salt tolerance of transgenic plants, in terms of seed germination, root growth, and electrolyte leakage during seedling establishment. Under NaCl stress, transgenic plants retained K+ and limited the accumulation of Na+. PeJRL-transgenic lines increased Na+ extrusion, which was associated with the upward regulation of SOS1, AHA1, and AHA2 genes encoding plasma membrane Na+/proton (H+) antiporter and H+-pumps. The activated H+-ATPases in PeJRL-overexpressed plants restricted the channel-mediated loss of K+ that was activated by NaCl-induced depolarization. Under salt stress, PeJRL–transgenic Arabidopsis maintained reactive oxygen species (ROS) homeostasis by activating the antioxidant enzymes and reducing the production of O2− through downregulation of NADPH oxidases. Of note, the PeJRL-transgenic Arabidopsis repressed abscisic acid (ABA) biosynthesis, thus reducing the ABA-elicited ROS production and the oxidative damage during the period of salt stress. A schematic model was proposed to show the mediation of PeJRL on ABA response, and ionic and ROS homeostasis under NaCl stress.
Anterior Maxilla Augmentation Using Palatal Bone Block with Platelet-Rich Fibrin: A Controlled Trial.

PURPOSE This study was conducted to characterize clinically and radiographically the effect of using platelet-rich fibrin (PRF) autologous graft on the augmentation results of autogenous palatal bone blocks. MATERIALS AND METHODS Patients who suffered from horizontal alveolar bone defects in the anterior maxillary ridge (≤ 4.5 mm) with one or two missing teeth were augmented with autogenous palatal bone blocks 4 months prior to implant placement. PRF was used to cover the block in the test group, while only the block was used in the control. Bone width was measured with a manual caliper preaugmentation (t0), and at 0 (t1) and 4 months (t2). Cone beam computed tomography (CBCT) scans were performed at t0 and t2. Nonparametric tests (Mann-Whitney, Friedman's, and Wilcoxon signed-rank tests) were used for the comparisons. The significance level was set at P ≤ . 05. RESULTS In the 14 sites (12 patients), all but one autograft (from the control group) integrated successfully after 4 months. No statistically significant difference was found between demographic data in the two groups. There was a statistically significant increase in the buccopalatal bone width in both groups by time as measured by CBCT as well as the manual caliper. The test group showed statistically significantly lower mean graft resorption than the control group (test, 0.8 ± 0.6 mm; control, 1.6 ± 0.9 mm; P = .006). CONCLUSION Autogenous palatal bone block surface resorption is significantly decreased by the use of PRF coverage.