Miracle-dz/UniFAR-FTUs huggingface dataset:
Contribution of both positive selection and relaxation of selective constraints to degeneration of flyability during geese domestication

Flyability is the most discrepant trait between modern-day geese and their wild ancestors, and the degeneration of flyability is a key marker of the successful domestication of wild geese. In light of the relatively short history of domestic geese, intense artificial selection is thought to play an important role in the degeneration of flyability. However, the underlying mechanism behind this phenomenon has seldom been investigated. In this study, we applied a molecular evolutionary approach to the evaluation of partial breeds of domestic geese in order to look for genes involved in the selection pressure toward degeneration of flyability. The haplotype networks, pairwise fixation index (FST) values, and analysis of molecular variance results all clearly illustrated a population variance between Landes geese and partial Chinese domestic geese. We also detected signatures of positive artificial selection in the COX2 and COX3 genes, and related selection in the HBB gene. Our results support the independent origins of partial European domestic geese and Chinese domestic geese. In addition, both positive artificial selection and the relaxation of functional constraints appeared to play important roles in the degeneration of flyability in domestic geese.
Musculoskeletal Medicine: An Assessment of the Attitudes and Knowledge of Medical Students at Harvard Medical School

Purpose To assess medical students’ knowledge and clinical confidence in musculoskeletal medicine as well as their attitudes toward the education they receive in this specialty. Method A cross-sectional survey of students in all four years of Harvard Medical School was conducted during the 2005–2006 academic year. Participants were asked to fill out a 30-question survey and a nationally validated basic competency exam in musculoskeletal medicine. Results The response rate was 74% (449/608). Medical students rated musculoskeletal education to be of major importance (3.8/5) but rated the amount of curriculum time spent on musculoskeletal medicine as poor (2.1/5). Third-year students felt a low to adequate level of confidence in performing a musculoskeletal physical examination (2.7/5) and failed to demonstrate cognitive mastery in musculoskeletal medicine (passing rate on competency exam: 7%), whereas fourth-year students reported a similar level of confidence (2.7/5) and exhibited a higher passing rate (26%). Increasing exposure to the subject by taking clinical electives resulted in greater clinical confidence and enhanced performance on the exam (P < .001). Students’ feedback suggested that musculoskeletal education can be better integrated into the preclinical curriculum, more time should be spent in the field, and more focus should be placed on common clinical conditions. Conclusions These findings, which are consistent with those from other schools, suggest that medical students do not feel adequately prepared in musculoskeletal medicine and lack both clinical confidence and cognitive mastery in the field. Implementing a four-year integrated musculoskeletal curriculum is one way that medical schools can address this concern.
An analysis of relationship between human capital and economic growth

Human capital in every sense is one of the fundamental factors of development. No country can achieve sustainable economic development without substantial investment in human capital. The relationship between economic growth and human capital has been long recognized. Many studies especially in Western economies have shown that human capital has a positive impact on economic growth. However, economic growth also has a strong effect on human capital outcomes, both through private expenditures and government programs. Thus, higher incomes facilitate the achievement of other crucial human development objectives; it also has an indirect effect on human development. In Malaysia, human capital investment is becoming an important aspect of the development agenda for which a large percentage of its expenditure is being allocated. This study attempts to analyze the relationship between human capital and economic growth in Malaysia using an augmented aggregate production function growth model, we apply the bounds testing (ARDL) approach to cointegration which is more appropriate for estimation in small sample studies. Human capital is represented by life expectancy at birth and public expenditure on education, while economic growth is measured using real gross domestic product. The data used for the analysis are gathered from various government agencies and world reports and the coverage is from 1980 to 2009. The study reveals that the traditional inputs i.e capital and labour are statistically significant in both the long-run and the short-run, having positive effects on economic growth in Malaysia. Government expenditure on education is only significant at 12.6 per cent level, while life expectancy is significant at 16.1 per cent. In other words, economic growth in Malaysia is very much input-driven i.e. by adding more and more resources into the same production function. Such growth is hard work and by the law of diminishing returns, cannot be sustained indefinitely. A large budget allocated to education does not translate into improvement in the quality of workforce and production process, innovation and technological advancement. Empirical results in this paper suggest that Malaysian education system must produce more efficient workforce to increase the contribution of human capital to its economic growth. A large budget allocation to education sector must be utilized optimally through providing education that tailored to the nation’s need. Further human capital investment in the labour market is also needed to produce skilled workers. This argument is further strengthened when we look at the objective for improving human capital which is not merely to achieve a high level of economic growth but also to fulfill social needs. [Idris Jajri, Rahmah Ismail. An Analysis of Relationship Between Human Capital and Economic Growth. Life Sci J 2012;9(4):3735-3742] (ISSN:1097-8135). http://www.lifesciencesite. com. 554
The thermoexpandable nitinol stent: a long-term alternative in patients without nephropathy or malignancy

Abstract Objective: The aim of this study was to investigate the long-term outcome of a thermoexpandable nickel–titanium nitinol ureteral stent (Memokath 051™) and to identify individual risk factors for failure. Materials and methods: This retrospective single-centre study included 125 patients who underwent implantation of the self-expandable Memokath 051 stent. Complications, indwelling time and reason for explantation were recorded. Analyses were stratified by gender, age, body mass index, American Society of Anesthesiologists score, estimated glomerular filtration rate (eGFR), side, localization and cause of the stricture. Results: In total, 91 out of 125 patients (73%) were available for analysis. Median indwelling time was 355 days (range 7–2125 days). Most stents were removed because of dislocation (42%) or occlusion (40%). Stent removal was rarely performed because of infection (3%). Patients with sufficient renal function (eGFR ≥60 ml/min/1.73 m²) showed increased indwelling times compared with those with nephropathy (386 vs 317 days; p < 0.01). Patients with active malignant disease showed reduced patency time compared with strictures of benign origin (455 vs 190 days; p < 0.01). Conclusions: This thermoexpandable nitinol stent offers safe mid-term treatment of ureteric strictures, especially in patients without active malignancy and with good renal function.
Temporal features of adult neurogenesis: differences and similarities across mammalian species

Production of new neurons continues throughout life in most invertebrates and vertebrates like crustaceans, fishes, reptiles, birds, and mammals including humans. Most studies have been carried out on rodent models and demonstrated that adult neurogenesis is located mainly in two structures, the dentate gyrus (DG) of the hippocampus and the sub-ventricular zone (SVZ). If adult neurogenesis is well preserved throughout evolution, yet there are however some features which differ between species. The present review proposes to target similarities and differences in the mechanism of mammalian adult neurogenesis by comparing selected species including humans. We will highlight the cellular composition and morphological organization of the SVZ in primates which differs from that of rodents and may be of functional relevance. We will particularly focus on the dynamic of neuronal maturation in rodents, primates, and humans but also in sheep which appears to be an interesting model due to its similarities with the primate brain.
Availability and perceived effectiveness of public health activities in the nation's most populous communities.

OBJECTIVES We examined the availability and perceived effectiveness of 20 basic public health activities in the communities where most Americans reside. METHODS A self-administered questionnaire was mailed to the 497 directors of US local health departments serving at least 100 000 residents. RESULTS On average, two thirds of the 20 public health activities were performed in the local jurisdictions surveyed, and the perceived effectiveness rating averaged 35% of the maximum possible. In multivariate models, availability of public health activities varied significantly according to population size, socioeconomic measures, local health department spending, and presence of local boards of health. CONCLUSIONS Local public health capacity varies widely across the nation's most populous communities, highlighting the need for targeted improvement efforts.
Antithrombin ‘DREUX’ (Lys114Glu): A Variant with Complete Loss of Heparin Affinity

Summary Here we report the finding of a new natural antithrombin mutation that confirms the critical contribution of lysine 114 to the binding of the core heparin pentasaccharide, with the replacement of lysine 114 by glutamate causing a complete loss in affinity. The variant was identified in a father and son, the father having been investigated for an episode of cerebral ischaemia associated with hypercholesterolaemia. The variant forms SDS-stable complexes with activated factor X (fXa) and its thermal stability and rate of factor Xa inhibition in the absence of heparin are identical to those of normal antithrombin. Normal antithrombin binds to the high affinity heparin pentasaccharide with a Kd of 1nM, as detected by a 45% change in intrinsic fluorescence, resulting in a 230-fold increase in rate of factor Xa inhibition. However, no change in fluorescence was detected for the variant when titrated with heparin or the heparin pentasaccharide, nor was there detectable activation towards factor Xa, indicating a complete loss of heparin binding.
Mortality and socioeconomic deprivation in census tracts of an urban setting in Southern Europe

In southern European cities, research on deprivation and mortality inequalities using small-area analysis is recent. In many countries, the census tract (CT) is the smallest territorial unit for which population data are available. The aim of this study was to examine the association between mortality from all causes and socioeconomic deprivation in CTs in Barcelona (Spain). A cross-sectional ecologic study was carried out using mortality data for 1987–1995 and 1991 census variables. Mortality data were obtained from death certificates. Socioeconomic deprivation indicators were drawn from the census and included unemployment, inadequate education, and low social class. They were correlated, and a deprivation index was elaborated with them. The analysis was descriptive, and multivariate Poisson regression models were adjusted. The most deprived CTs tend to present higher mortality (49.7% of CT in the quartile associated with greatest deprivation were included in the top male mortality quartile and 40.4% in the top female mortality quartile), whereas the less deprived ones present lower mortality. For male mortality, the risk of dying among those in the quartile representing most deprivation is from 25 to 29% higher (depending on the indicator chosen) than the least deprived quartile, and for women, it is from 12 to 14% higher. We concluded that the mortality from all causes in the CT of a southern European city has shown a clear positive association with a variety of socioeconomic deprivation indicators drawn from the census. Studies of this nature may help to orient more specific studies in which CTs are grouped together as a function of particular population and/or health characteristics.
Fluid loss and body composition of elite Kuwaiti soccer players during a soccer match.

AIM The aim of this study was to assess the effect of the hot weather conditions of Kuwait on fluid loss (FL) and body composition of Kuwaiti soccer players. METHODS During 5 preseason soccer games, 10 elite Kuwaiti soccer players participated in this study. The age and physical characteristics (mean+/-SD) of the subjects were: age 24+/-4.7 years; height 173.4+/-5.2 cm; mass 68.2+/-7 kg; and the percent body fat 12.3+/-3.7%, the mean temperature 45.4+/-2 degrees C, mean humidity was 23.6+/-4.2%. Measurements were taken at the beginning, half time, and the end of each game; FL was measured by body weight changes. Urine specific gravity was used to determine the state of hydration. Body composition was measured using Biodynamics Model 310e Body Composition Analyzer. RESULTS There was a significant difference in body weight changes between 1st and 2nd half at P>0.05. The mean value of the FL at the end of the game was 3.1+/-1.4 L; indicating no significant difference in FL between 1st and 2nd half at P>0.05). The mean value urine specific gravity at the end game was 1.026+/-0.002; there was a significant difference between first, and second half for urine specific gravity (P>0.05). CONCLUSIONS Results of this study indicated that the subjects did not consume enough fluid to offset FL. Players and coaches involved with activities in hot humid environments should pay closer attention to FL and body weight changes which occur during physical activity. Special care should always be taken to insure players consume extra water prior to each training session or game.
A Reduced Set of Air Plasma Reactions for Nanosecond Pulsed Plasmas

The reduction of a mechanism describing plasma chemistry for dry air has been carried out for conditions of nanosecond-pulsed discharge plasmas. The discharge conditions include both diffuse glow and filamentary spark modes. A reduced set for glow discharges is found to have inelastic electron-impact reactions of N<sub>2</sub> and O<sub>2</sub>, quenching of excited N<sub>2</sub>, electron attachment to O<sub>2</sub><sup>-</sup>, ion conversion between N<sub>2</sub><sup>+</sup>, N<sub>4</sub><sup>+</sup>, O<sub>4</sub><sup>+</sup>, O<sub>2</sub><sup>+</sup>·N<sub>2</sub>, and O<sub>2</sub><sup>+</sup>, and ion-ion and electron-ion recombination. When the discharge is in a filamentary mode, additional reactions are required beyond those for the glow discharge as a result of the increased electron number density and high levels of molecular dissociation. Those additional reactions include electron-impact excitation of N and O, quenching of excited N<sub>2</sub> by N and O, and associative ionization by collisions between excited N<sub>2</sub>. A significant reduction in the number of species and reactions is obtained without compromising the predicted species' number densities and temperature. These results provide researchers with reduced kinetic mechanisms that high-fidelity simulations could rely on to save computational time.
Deletion of an amino-terminal sequence beta-catenin in vivo and promotes hyperphosporylation of the adenomatous polyposis coli tumor suppressor protein

Regulation of cell adhesion and cell signaling by beta-catenin occurs through a mechanism likely involving the targeted degradation of the protein. Deletional analysis was used to generate a beta-catenin refractory to rapid turnover and to examine its effects on complexes containing either cadherin or the adenomatous polyposis coli (APC) protein. The results show that amino-terminal deletion of beta-catenin results in a protein with increased stability that acts in a dominant fashion with respect to wild-type beta-catenin. Constitutive expression in AtT20 cells of a beta-catenin lacking 89 N-terminal amino acids (deltaN89beta-catenin) resulted in severely reduced levels of the more labile wild-type beta-catenin. The mutant beta-catenin was expressed at endogenous levels but displaced the vast majority of wild-type beta-catenin associated with N-cadherin. The deltaN89beta-catenin accumulated on the APC protein to a level 10-fold over that of wild-type beta-catenin and recruited a kinase into the APC complex. The kinase was highly active toward APC in vitro and promoted a sodium dodecyl sulfate gel band shift that was also evident for endogenous APC from cells expressing the mutant beta-catenin. Unlike wild-type beta-catenin, which partitions solely as part of a high-molecular-weight complex, the deltaN89 mutant protein also fractionated as a stable monomer, indicating that it had escaped the requirement to associate with other proteins. That similar N-terminal mutants of beta-catenin have been implicated in cellular transformation suggests that their abnormal association with APC may, in part, be responsible for this phenotype.
New methodology for surface potential decay measurements: application to study charge injection dynamics on polypropylene films

Our work shows, through measurements on polypropylene films, the validity of surface potential measurements as a technique for dielectric properties analysis. We propose a representation of the decay and potential return curves that separates different components of dielectric response, by using tdv/dt vs log t plots. Using this transformation, and varying some experimental conditions we have identified different contributions to the potential decay. From our experimental observations we suggest a qualitative interpretation of the initial seconds of potential decay for negative corona charged samples that uses a model controlled by surface trap emission and charge exchanges between corona discharge and the insulator surface.
Prospective multiple outcomes study of outpatient lumbar microdiscectomy: should 75 to 80% success rates be the norm?

OBJECT The authors assessed the efficacy and outcomes of lumbar microdiscectomy performed on an outpatient basis by administering six questionnaires before and at five time points after surgery. The results were compared with those reported in literature in which the success rates vary between 70% and 80% and in excess of 90%. The authors use the methodology and data derived from their study to evaluate critically the relevance of these two categories of success rates. METHODS This is a prospective study of 212 consecutive, eligible patients who underwent outpatient microscopic discectomy for the treatment of lumbar disc herniation: no previous lumbar lesion had been treated. Data were collected from questionnaires given to the patients before and at five time points after surgery, including at a variable final follow-up examination (mean 2 years postoperatively). Data were collated and analyzed independently by individuals other than the operating surgeons. In both bi- and multivariate analyses, only two preoperative parameters were prognostically significant. The first factor was Workers' Compensation status, which had a negative effect on outcome. The second factor was patient age, which also had a negative effect and was linear with increasing age between 25 years and 56 years--that is, the ages most commonly encountered in cases of herniated disc. Successful outcome rates were as follows: leg pain relief according to a visual analog scale (VAS), 80%; back pain relief (VAS), 77%; Oswestry Low Back Disability Index, 78%; satisfaction with the results of surgery, 76%; return to normal daily activities, 65%; and return to work, 61%. CONCLUSIONS The findings of this study support the evidence that lumbar microdiscectomy performed on an outpatient basis is a very safe and effective means of treating sciatic pain due to disc herniation. The authors believe that their outcome success rates of 75 to 80% are more realistic than those of 90% or more found in some reports.
The use of canines in the detection of human cancers.

OBJECTIVES To determine whether canines could be trained to identify patients with cancer by sniffing the urine obtained from a patient with breast or prostate cancer from among samples obtained from healthy volunteers. DESIGN Dogs of different breeds were trained by their owners to detect the urine sample from a patient with cancer from among 6 other age- and sex-matched healthy volunteers. After the training was completed, using new samples, 2 test runs were used for each patient with breast cancer and three runs for the patients with prostate cancer against the same matched samples. The configuration of the samples was different for each run. A total of 18 and 33 runs were carried out, respectively. RESULTS For each cohort, specificity and sensitivity were measured. In the breast cancer tests, of 6 dogs, only 2 performed better than chance in specificity and none were more sensitive than chance. For the prostate sample testing, 4 dogs were used. Two performed significantly better than chance in specificity and none in sensitivity. CONCLUSIONS Although this study did not produce the outcomes desired, the literature supports a potential to use canines for human cancer detection. Better management of urine samples and a more stringent training protocol during our study may have provided new evidence as to the feasibility of using canines for cancer detection. A comparison of the 3 dog cancer scenting studies is also presented.
The Conserved Glu in the Cyclotide Cycloviolacin O2 Has a Key Structural Role

Cyclotides are a large family of plant peptides that are characterised by a head‐to‐tail circular backbone and three disulfide bonds that are arranged in a cystine knot. This unique structural feature, which is referred to as a cyclic cystine knot, gives the cyclotides remarkable stability against chemical and biological degradation. In addition to their natural function as insecticides for plant defence, the cyclotides have a range of bioactivities with pharmaceutical relevance, including cytotoxicity against cancer cell lines. A glutamic acid residue, aside from the invariable disulfide array, is the most conserved feature throughout the cyclotide family, and it has recently been shown to be crucial for biological activity. Here we have used solution‐state NMR spectroscopy to determine the three‐dimensional structures of the potent cytotoxic cyclotide cycloviolacin O2, and an inactive analogue in which this conserved glutamic acid has been methylated. The structures of the peptides show that the glutamic acid has a key structural role in coordinating a set of hydrogen bonds in native cycloviolacin O2; this interaction is disrupted in the methylated analogue. The proposed mechanism of action of cyclotides is membrane disruption and these results suggest that the glutamic acid is linked to cyclotide function by stabilising the structure to allow efficient aggregation in membranes, rather than in a direct interaction with a target receptor.