Miracle-dz/UniFAR-FTUs huggingface dataset:
Reproductive and developmental toxicity of carbon-based nanomaterials: A literature review

Abstract We summarized the findings of reproductive and developmental toxicity studies on carbon-based nanomaterials (CNMs). Placental transfer of fullerenes in rats and single-walled (SW) and multi-walled (MW) CNTs in mice was shown after intravenous injection. SWCNTs appeared to be embryolethal and teratogenic in mice when given by intravenous injection and induced death and growth retardation in chicken embryos. In mice-administered MWCNTs, fetal malformations after intravenous and intraperitoneal injections and intratracheal instillation, fetal loss after intravenous injection, behavioral changes in offspring after intraperitoneal injection, and a delay in the delivery of the first litter after intratracheal instillation were reported. Oral gavage of MWCNTs had no developmental toxicity in mice and rats. MWCNTs produced morphological defects, developmental arrest, and death in zebrafish embryos. Intratracheal instillation of carbon black (CB) induced testicular toxicity in adult mice. Maternal airway exposure to CB in gestation had testicular toxicity and altered postnatal behavior, renal development, immune and genotoxic responses, and brain morphology in mouse offspring. Nanodiamonds and graphite nanoparticles inhibited vasculogenesis and/or angiogenesis in chicken embryos. Graphene oxide (GO) induced malformations in zebrafish embryos. Intravenous injection of reduced GO during late gestation caused maternal death and abortion in mice. Oral administration of GO during lactation caused growth retardation of offspring. Overall, the available data provide initial information on the potential reproductive and developmental toxicity of CNMs. However, confirmatory studies using well-characterized CNMs, state-of-the-art study protocol and appropriate route of exposure, are required to clarify the findings and provide information suitable for risk assessment.
Retroperitoneal Repair of Abdominal Aortic Aneurysm Reduces Bowel Dysfunction

Objective: To assess the effect of intestinal manipulation and mesenteric traction on gastro-intestinal function and postoperative recovery in patients undergoing abdominal aortic aneurysm (AAA) repair. Methods: Thirty-five patients undergoing AAA repair were randomised into 3 groups. Group I (n = 11) had repair via retroperitoneal approach while Group II (n = 12) and Group III (n = 12) were repaired via transperitoneal approach with bowel packed within the peritoneal cavity or exteriorised in a bowel bag respectively. Gastric emptying was measured pre-operatively (day 0), day 1 and day 3 using paracetamol absorption test (PAT) and area under curve (PAUC) was calculated. Intestinal permeability was measured using the Lactulose-Mannitol test. Results: Aneurysm size, operation time and PAT (on day 0 and day 3) were similar in the three groups. On day 1, the PAUC was significantly higher in Group I, when compared with Group II and Group III (P = .02). Resumption of diet was also significantly earlier in Group I as compared to Group II and Group III. The intestinal permeability was significantly increased in Group II and Group III at day 1 when compared with day 0, with no significant increase in Group I. Retroperitoneal repair was also associated with significantly shorter intensive care unit (P = .04) and hospital stay (P = .047), when compared with the combined transperitoneal repair group (Group II and III). Conclusion: Retroperitoneal AAA repair minimises intestinal dysfunction and may lead to quicker patient recovery when compared to transperitoneal repair.
Diagnostic, prognostic, and predictive biomarkers in pancreatic cancer

Serum CA 19‐9 is the only FDA approved biomarker recommended for use in the routine management of pancreatic ductal adenocarcinoma (PDA). Over 2,000 biomarker studies related to pancreatic cancer appear in the literature, highlighting the need to discover and develop improved tests. Diagnostic biomarkers have implications for early detection of PDA, prognostic markers predict patient survival and recurrence patterns, and predictive markers can help personalize treatment regimens. J. Surg. Oncol. 2013;107:15–22. © 2012 Wiley Periodicals, Inc.
Small-Dose Pentobarbital Enhances Synaptic Transmission in Rat Hippocampus

We investigated the contribution of bicarbonate ion, &ggr;-aminobutyric acid-A (GABAA) receptors, and N-methyl-d-aspartate (NMDA) receptors to pentobarbital-induced enhancement of excitatory synaptic transmission in the hippocampal slice. Transverse hippocampal slices (400 &mgr;m thick) were prepared from 20- to 30-day-old Sprague-Dawley rats and maintained in an interface chamber perfused with warmed (35°C) oxygenated artificial cerebrospinal fluid. Extracellular field potentials, evoked by orthodromic paired-pulse stimulation of the Schaffer collateral CA1 pathway, were analyzed for the population spike (PS) amplitude. Pentobarbital had a concentration-dependent, biphasic effect on PS amplitudes, which were increased approximately twofold (P < 0.001) when the slice was exposed to pentobarbital concentrations of 1 and 5 &mgr;M and depressed at drug concentrations larger than 10 &mgr;M. Pentobarbital (5 &mgr;M) did not increase the PS amplitude when stimulation was stopped during exposure to the drug. The enhancement of PS amplitude was suppressed in the presence of 10 &mgr;M acetazolamide, a nonselective carbonic anhydrase inhibitor, and when the slice was bathed in CO2/HCO3−-free artificial cerebrospinal fluid. Pretreatment with 1 &mgr;M picrotoxin, a GABAA receptor antagonist, or 5 &mgr;M 2-amino-5-phosphopentanoic acid, a specific NMDA receptor antagonist, also suppressed enhancement of PS amplitude by 5 &mgr;M pentobarbital. The results suggest that small concentrations of pentobarbital (1 and 5 &mgr;M) enhance synaptic transmission through mechanisms involving GABAA and NMDA receptors and the HCO3− ion.
The Effectiveness of Bilateral Cochlear Implants for Severe-to-Profound Deafness in Children: A Systematic Review

Objective: To assess the clinical effectiveness of bilateral cochlear implantation compared with unilateral cochlear implantation alone or with a contralateral hearing aid (bimodal stimulation), in children with severe-to-profound hearing loss. Recently, the National Institute for Health and Clinical Excellence (NICE) in the U.K. has conducted a systematic review on cochlear implantation. We decided to update the pediatric part of the NICE review. Data Sources: The electronic databases MEDLINE and Embase were searched for European, North American, and Australasian studies published between October 2006 and June 2009. Reference lists of the included articles were also searched for relevant articles. Study Selection: Studies were included if they comprised data on comparisons between bilateral cochlear implantation and unilateral cochlear implantation and/or bilateral cochlear implantation and bimodal stimulation, in children with severe-to-profound sensorineural hearing loss. The following outcome measures were analyzed: audiological, speech perception, speech production, functional capacities, health-related quality of life, and/or educational outcomes. Data Extraction: Characteristics of the participants, interventions, outcomes, and methodological comments were entered into data extraction forms and the level of evidence was assessed. Data Synthesis: Results were standardized for each outcome measure by calculating a standardized mean difference (effect size). Conclusion: Effect sizes could not be pooled because of the heterogeneity of the studies. Therefore, we presented the results qualitatively. Although the level of evidence was low, the advantages of bilateral cochlear implants corresponded with the primary benefits of bilateral hearing, that is, improved speech perception in quiet and noise. Localization results were less consistent. No data on audiologic, speech production, or educational outcomes were available.
Mycotoxin profiles in the grain of Triticum monococcum, Triticum dicoccum and Triticum spelta after head infection with Fusarium culmorum.

BACKGROUND The aim of study was to investigate mycotoxin profiles in the grain of spring lines of Triticum monococcum (12 lines), T. dicoccum (13 lines) and T. spelta (five lines), in comparison to the T. aestivum cultivar Sumai-3 which is resistant to Fusarium head blight. The grain was obtained from control heads and heads artificially inoculated in the field with Fusarium culmorum. Mycotoxins were determined by LC-MS/MS. RESULTS A total of 11 toxins were identified in control grain samples. Deoxynivalenol (DON) concentrations exceeded 0.5 mg kg(-1) in only three samples of T. monococcum grain and in one sample of T. dicoccum grain. Inoculation with F. culmorum resulted in a substantial increase in the concentrations of DON (to 63 mg kg(-1)) in the T. monococcum and DON-3-glucoside (to 5.6 mg kg(-1)) in the T. dicoccum. Inoculation contributed to a drop in tentoxin levels (by 57% in T. spelta) and to an increase of cyclodepsipeptide concentrations (in particular enniatins B and B(1)) being two-fold (T. monococcum) to four-fold (T. dicoccum) higher on average than in control samples. The Sumai-3 responded to inoculation with nearly a two-fold drop in the levels of the cyclodepsipeptides. CONCLUSION The results of a discriminant analysis for all identified toxins indicate that einkorn, emmer and spelt differ significantly with regard to the mycotoxin profiles of their grain.
Habitat selection by foraging macaroni penguins correlates with hematocrit, an index of aerobic condition

Reproductive investment during the chick-rearing period is an important contributor to lifetime fitness. Key to chick-rearing is the success of parental foraging, as food deliveries affect chick growth and survival up until fledging. For seabirds, oceanographic conditions including factors such as sea surface temperature are known to influence foraging decisions, but few studies have examined the physiological variables that might affect those decisions. We used global positioning systems (GPS), time-depth recorders (TDR), and physiological sampling techniques to explore links between ocean temperature, diving behavior, and foraging success in chick-rearing female macaroni penguins Eudyptes chrysolophus. We then explored correlations between these foraging variables and measures of individual physiological condition, specifically aerobic capacity (hematocrit) and metabolic state (corticosterone). In GPS-tracked penguins, 2 principal foraging tactics were observed: penguins made deep dives in cool, near-shore areas surrounding the breeding colony at Bird Island, South Georgia, or they traveled farther to dive shallower in warmer shelf-break areas. TDR-equipped penguins showed similar patterns. Blood sampling of TDR penguins at the onset of trips revealed strong positive correlations between hematocrit and the mean duration of foraging trips, the ocean temperature experienced during these trips, and the relative efficiency of foraging activity in terms of the number of foraging behaviors recorded per dive. These results suggest that aerobic capacity might be an important determinant of foraging trip range, as well as workload. Corticosterone was unrelated to diving behavior, which counters previous studies examining the effects of experimental increases of this hormone on foraging behavior, and we discuss reasons for this disparity.
A Pragmatic Trial in the Rio de Janeiro Subway to Capture Smokers for a Quitline: Methodological Challenges and Opportunities

According to the World Health Organization, smoking is an important cause of death worldwide. To encourage smoking cessation, persuasive messages can be used to raise smokers' risk perception. This article discusses challenges and solutions in designing a study to evaluate the effect of two different communication strategies (“gains from quitting” vs. “losses from continuing smoking”) in encouraging calls to a quitline. The authors conducted an intervention study in two subway stations for 4 weeks, considering only 1 strategy per station. Large posters containing non–age-specific images and texts, on the basis of the theme“shortness of breath,” were displayed on central dividing columns on the boarding platforms. Call rates from the selected stations, and respective rate ratios, overall and per study week, were calculated. Passengers who were smokers, exposed to the positive-content message, called on average 1.7 times more often than did those exposed to the negative-content message (p = .01). Moreover, call rate ratios did not decline over the 4 weeks of the study (p = .40). The effectiveness findings suggest that antismoking campaigns could use positive-content messages in order to recruit a larger smoker population. The proposed methodology can also be used to evaluate effectiveness of messages for “capturing” individuals with other health problems (e.g., alcohol abuse), thereby increasing its potential impact.
Planetary bioresources and astroecology: 1. Planetary microcosm bioassays of Martian and carbonaceous chondrite materials: Nutrients, electrolyte solutions, and algal and plant responses

The biological fertilities of planetary materials can be assessed using microcosms based on meteorites. This study applies microcosm tests to martian meteorites and analogues and to carbonaceous chondrites. The biological fertilities of these materials are rated based on the soluble electrolyte nutrients, the growth of mesophile and cold-tolerant algae, and plant tissue cultures. The results show that the meteorites, in particular the Murchison CM2 carbonaceous chondrite and DaG 476 martian shergottite, contain high levels of water-extractable Ca, Mg, and SO4‐S. The martian meteorites DaG 476 and EETA 79001 also contain higher levels of extractable essential nutrients NO3‐N (0.013‐0.017 g kg −1 ) and PO4‐P (0.019‐ 0.046 g kg −1 ) than the terrestrial analogues. The yields of most of the water-extractable electrolytes vary only by factors of 2‐3 under a wide range of planetary conditions. However, the longterm extractable phosphate increases significantly under a CO2 atmosphere. The biological yields of algae and plant tissue cultures correlate with extractable NO3‐N and PO4‐P, identifying these as the limiting nutrients. Mesophilic algae and Asparagus officinalis cultures are identified as useful bioassay agents. A fertility rating system based on microcosm tests is proposed. The results rate the fertilities in the order martian basalts > terrestrial basalt, agricultural soil > carbonaceous chondrites, lava ash > cumulate igneous rock. The results demonstrate the application of planetary microcosms in experimental astroecology to rate planetary materials as targets for astrobiology exploration and as potential space bioresources. For example, the extractable materials in Murchison suggest that concentrated internal solutions in carbonaceous asteroids (3.8 mol L −1 electrolytes and 10 g L −1 organics) can support and disperse microorganisms introduced by natural or directed panspermia in early solar systems. The results also suggest that carbonaceous asteroids and martian basalts can serve as potential future resources for substantial biological populations in the Solar System. c � 2002 Elsevier Science (USA)
A viscoelastic damage model with applications to stable and unstable fracturing

SUMMARY A viscoelastic damage rheology model is presented that provides a generalization of Maxwell viscoelasticity to a non-linear continuum mechanics framework incorporating material degradation and recovery, transition from stable to unstable fracturing and gradual accumulation of non-reversible deformation. The model is a further development of the damage rheology framework of Lyakhovsky et al. for evolving effective elasticity. The framework provides a quantitative treatment for macroscopic effects of evolving distributed cracking with local density represented by an intensive state variable. The formulation, based on thermodynamic principles, leads to a system of kinetic equations for the evolution of damage. An effective viscosity inversely proportional to the rate of damage increase is introduced to account for gradual accumulation of irreversible deformation due to dissipative processes. A power-law relation between the damage variable and elastic moduli leads to a non-linear coupling between the rate of damage evolution and the damage variable itself. This allows the model to reproduce a transition from stable to unstable fracturing of brittle rocks and the Kaiser effect. 3-D numerical simulations based on the model formulation for homogeneous and heterogeneous materials account for the main features of rock behaviour under large strain. The model coefficients are constrained, using triaxial laboratory experiments with low-porosity Westerly granite and high-porosity Berea sandstone samples.
Consonant clusters in disordered speech: constraints and correspondence patterns

ABSTRACT Comparison of patterns of cluster realization from 47 children ranging in age from 3;4 to 6;8 with functional (non-organic) speech disorders with those reported in the literature for normal acquisition reveals that these patterns are essentially the same for both groups. Using a two-level generative phonology for children's independent systems, further analysis of cluster realizations by means of feature geometry and under-specification theory reveals that there are systematic and principled relationships between adult representations of clusters and children's underlying representations and between children's underlying representations and their phonetic representations. With special emphasis on coalescence phenomena, it is suggested that the apparent diversity in children's cluster realizations can be reduced to four constraints on the form of underlying and phonetic representations.
Gsx transcription factors repress Iroquois gene expression

We have previously shown that the Gsx family homeobox gene Gsh2 is part of the regulatory network specifying dorsoventral pattern of primary neurons in the developing amphibian embryo. Here, we investigate the role of Gsx transcription factors in regulating the transcription of Iroquois family homeobox genes in the amphibian neural plate. Iroquois genes are key regulators of neural patterning and their expression is coincident with that of the Gsx genes during open neural plate stages. We show that Gsx proteins repress Iroquois expression in the embryo and conversely, inhibition of Gsx activity with either antisense morpholino oligos or an anti‐morphic Gsx protein up‐regulates Iroquois expression. These data indicate that Gsx factors act as negative regulators of Iroquois gene expression in the amphibian neural plate and support a model in which the Gsx proteins promote neuronal differentiation by repressing the expression of known inhibitors of neuronal differentiation such as Iro3. Developmental Dynamics 240:1422–1429, 2011. © 2011 Wiley‐Liss, Inc.
Genetic Diversity and Genetic Relationships of Purple Willow (Salix purpurea L.) from Natural Locations

In this study, the genetic diversity and structure of 13 natural locations of Salix purpurea were determined with the use of AFLP (amplified length polymorphism), RAPD (randomly amplified polymorphic DNA) and ISSR (inter-simple sequence repeats). The genetic relationships between 91 examined S. purpurea genotypes were evaluated by analyses of molecular variance (AMOVA), principal coordinates analyses (PCoA) and UPGMA (unweighted pair group method with arithmetic mean) dendrograms for both single marker types and a combination of all marker systems. The locations were assigned to distinct regions and the analysis of AMOVA (analysis of molecular variance) revealed a high genetic diversity within locations. The genetic diversity between both regions and locations was relatively low, but typical for many woody plant species. The results noted for the analyzed marker types were generally comparable with few differences in the genetic relationships among S. purpurea locations. A combination of several marker systems could thus be ideally suited to understand genetic diversity patterns of the species. This study makes the first attempt to broaden our knowledge of the genetic parameters of the purple willow (S. purpurea) from natural location for research and several applications, inter alia breeding purposes.
SDN controllers: A comparative study

The control plane is an essential part of the SDN architecture, so it is very important to give proper attention to any proposal or design of an SDN controller. During the past few years, several controllers have been developed and several studies have been done to evaluate, compare and test the performance of these controllers. In this paper, new controllers are tested, such as ONOS and Libfluid-based controllers (raw, base), using Cbench, an OpenFlow testing tool. Even though the results show that MUL, Beacon, and Maestro (in latency mode) are the best performing controllers; however, the selection of the best-fitted controller should be based on several criteria, per user requirements.
Dry matter and nitrogen accumulation and use in spring barley

During growth, kernel of cereals can be provided with carbohydrate and nitrogen (N) from the translocation of pre-anthesis accumulated reserves stored either in the vegetative plant parts or from current assimilation during kernel development. This study was conducted to assess the effects of nitrogen level and cultivars on dry matter and N accumulation and mobilization during pre-anthesis and post-anthesis. Twenty two-rowed spring barley ( Hordeum vulgare L.) cultivars were grown on a non-calcareous chernozem soil in four growing seasons (1995–1998) at Novi Sad (45°20′N, 15°51′E, 86 m a.s.l.) at two nitrogen levels. Dry matter accumulation before anthesis ranged from less than 50% in unfavorable to 90% in favorable growing conditions. Dry matter translocation occurred in favorable growing conditions only. Pre-anthesis accumulated N represented 57–92% and 54–129% of total N at maturity at the low and high N levels, respectively. Translocated N represented 41–85% and 37–153% of grain N at the low and high N level, respectively. N losses occurred in favorable growing conditions when anthesis N exceeded 150 kg/ha. N accumulation during grain filling was in negative correlation with dry matter and N accumulation before anthesis. The N harvest index was 0.57–0.63 and 0.71–0.74 in unfavorable and favorable growing conditions, respectively. Selection of genotypes with a higher ability of pre-anthesis reserve utilization or genotypes with longer leaf area duration after anthesis may be two possible solutions in spring barley breeding for Mediterranean growing conditions.
Likert scales: how to (ab)use them

Dipping my toe into the water of educational research, I have recently used Likert-type rating scales to measure student views on various educational interventions. Likert scales are commonly used to measure attitude, providing a range of responses to a given question or statement . Typically, there are 5 categories of response, from (for example) 1 1⁄4 strongly disagree to 5 1⁄4 strongly agree, although there are arguments in favour of scales with 7 or with an even number of response categories.