Miracle-dz/UniFAR-FTUs huggingface dataset:
Protein Kinase C-Mediated Modulation of FIH-1 Expression by the Homeodomain Protein CDP/Cut/Cux

ABSTRACT Under normoxia, FIH-1 (factor inhibiting HIF-1) inhibits the transcriptional activity of hypoxia-inducible factor (HIF); however, under such conditions, we observed a significant level of HIF activity in renal cell carcinoma (RCC). This phenomenon could be attributed to a decrease in the level of functional FIH that has been identified in our previous work. Nonetheless, the molecular mechanism of FIH regulation in cancer, in particular RCC, was unclear until now. In this communication, we have demonstrated that in RCC, the Cut-like homeodomain protein (CDP/Cut) is involved in FIH transcriptional regulation and is controlled by a specific signaling event involving protein kinase C (PKC) ζ. Furthermore, we have defined a unique CDP/Cut binding site on the FIH promoter. With chromatin immunoprecipitation assays, we show that CDP binds to the FIH-1 promoter in vivo and that this binding is PKC ζ dependent. Moreover, we have also defined a potential phosphorylation site in CDP (serine 987) that modulates FIH expression. CDP/Cut is a transcriptional repressor that decreases FIH-1 expression and subsequently leads to a decrease in the repressor activity of FIH-1. Without this repression, HIF activity increases, allowing for the increased transcription of the genes it regulates, such as the vascular endothelial growth factor and GLUT-1 genes. Both CDP and HIF levels are increased in several cancers and are responsible for the metastatic progression of the tumors. Taken together, our results suggest for the first time a potential connection between CDP and FIH that could lead to the development of future therapeutic interventions.
Variation and evolution of Class I Mhc in sexual and parthenogenetic geckos

We present the first Mhc class I sequences in geckos. We compared Mhc variation in gekkonid species that reproduce sexually (Hemidactylus frenatus, Lepidodactylus aureolineatus, L. moestus, L. sp. Arno, L. sp. Takapoto) to others reproducing parthenogenetically (H. garnotii, L. lugubris). These comparisons include the known maternal (L. moestus) and paternal (L. sp. Arno) ancestors of the asexual L. lugubris. Sequences similar to other vertebrate species were obtained from both nuclear and cDNA templates indicating that these sequences are derived from expressed class I Mhc loci. Southern blot analysis using gecko class I probes, revealed that parthenogenetic clonal lineages of independent evolutionary origin have no within-clone band variation at class I loci and that no detectable recombination between restriction sites had taken place. Variability in the sexual species was similar to mammalian taxa, i. e. class I genes are highly variable in outbreeding sexual populations. Sequence analysis of the alpha-2 domain of class I genes identified point mutations in a clonal lineage of L. lugubris which led to amino acid substitutions. Potential transspecific allelic lineages were also observed. The persistence of asexual lineages with little or no class I diversification over thousands of generations seems to argue against strong selection for Mhc multi-allelism caused by pathogen-Mhc allele specificity. On the other hand, the high level of heterozygosity in the parthenogenetic species (a consequence of their hybrid origin) may provide clonal lineages with adequate antigen presenting diversity to survive and compete with sexual relatives.
Neurocognitive effects of neurofeedback in adolescents with ADHD: a randomized controlled trial.

OBJECTIVE Neurofeedback aims to reduce symptoms of attention-deficit/hyperactivity disorder (ADHD), mainly attention problems. However, the additional influence of neurofeedback over treatment as usual (TAU) on neurocognitive functioning for adolescents with ADHD remains unclear. METHOD By using a multicenter parallel randomized controlled trial (RCT) design, male adolescents with a DSM-IV-TR diagnosis of ADHD (mean age = 16.1 years; range, 12-24) were randomized to receive either a combination of TAU and neurofeedback (n = 45) or TAU (n = 26). Randomization was computer generated and stratified by age group (ages 12 through 15, 16 through 20, and 21 through 24 years). The neurofeedback intervention consisted of approximately 37 sessions over a period of 25 weeks of theta/sensorimotor rhythm training on the vertex (Cz). Primary neurocognitive outcomes included performance parameters derived from the D2 Test of Attention, the Digit Span backward, the Stroop Color-Word Test and the Tower of London, all assessed preintervention and postintervention. Data were collected between December 2009 and July 2012. RESULTS At postintervention, outcomes of attention and/or motor speed were improved, with faster processing times for both intervention conditions and with medium to large effect sizes (range, ηp2 = .08-.54; P values < .023). In both groups, no improvements for higher executive functions were observed. Results might partly resemble practice effects. CONCLUSIONS Although neurocognitive outcomes improved in all adolescents receiving treatment for ADHD, no additional value for neurofeedback over TAU was observed. Hence, this study does not provide evidence for using theta/sensorimotor rhythm neurofeedback to enhance neurocognitive performance as additional intervention to TAU for adolescents with ADHD symptoms. TRIAL REGISTRATION Trialregister.nl identifier: 1759.
Experimental analysis of the relationship between charge movement components in skeletal muscle of Rana temporaria.

Experiments were performed to ascertain whether the monotonic (q beta) and delayed (q gamma) components of non‐linear charge in skeletal muscle membranes form a sequential system, or are the result of separate, independent processes. The non‐linear capacitance studied in a large number of fibres increased with fibre diameter. This dependence was attributable to tetracaine‐sensitive (q gamma) but not to tetracaine‐resistant (q beta and q alpha) charge. The kinetics and total quantity of q gamma charge moving in response to voltage steps from varying pre‐pulse potentials to a fixed probe potential remained constant despite variations in the size of the early q beta decay. The kinetics of the delayed (q gamma) charging current obtained from a single 20 mV depolarizing step were compared with the sum of the responses to two 10 mV steps adding to the same voltage excursion. The respective transients superimposed only if one of the 10 mV steps did not reach the voltage at which q gamma first appears. In the two preceding experiments, total charge was conserved. These results are consistent with separate and functionally independent q beta and q gamma systems of potential‐dependent charge, with q gamma residing in the transverse tubules and q beta on surface membrane. The findings can be discussed in terms of a contractile 'activator' with a steep sensitivity to voltage that begins only with depolarization beyond a level close to the actual mechanical threshold.
Inhaled short acting beta2-agonist use in asthma: regular vs as needed treatment.

BACKGROUND Inhaled beta2-adrenergic agonists delivered by inhalation are very widely used in asthma. There has been much controversy of the use and possible consequences of the use of these agents for regular, as opposed as-needed use in asthma. OBJECTIVES This review to assessed the clinical trial evidence to test whether using regular use of short-acting beta2-agonists reduced asthma control and pulmonary function; worsened symptoms, airway reactivity and quality of life; and increased the rate of exacerbations. SEARCH STRATEGY A search was carried out of the Cochrane Airways Group "Asthma and Wheez* RCT" register using the terms: regular AND [beta agonist OR bronchodilator OR salbutamol OR albuterol Or terbutaline OR isoproterenol OR reproterol OR fenoterol]. Bibliographies of existing trials were searched and primary trial authors and pharmaceutical companies were approached for additional trials. SELECTION CRITERIA Randomised controlled trials in which the short-acting beta2-agonist was given regularly in the experimental group, together with an inhaled bronchodilator for relief of symptoms ('rescue use'). The control group consisted of matching placebo inhaled regularly, with an inhaled bronchodilator for as-needed 'rescue use'. DATA COLLECTION AND ANALYSIS Data were extracted and quality assessments were made by both reviewers. Parallel group and cross-over trials were analysed separately. Where possible data were pooled using a fixed effects model. MAIN RESULTS Over 800 abstracts were identified, following a review, 60 papers were requested for full assessment by both reviewers. 34 trials from 30 papers met the entry criteria. Data from 31 outcomes were analyzable. There was little difference between the treatments for nearly all outcomes. In cross-over studies, evening peak flow was better with regular treatment, weighted mean difference (WMD) 13.1 l/min (95% confidence interval 24.3, 1.9). In contrast, the FEV1 was better with as-needed treatment (WMD 157 ml (95% CI: 123, 192). Bronchial hyper reactivity was slightly better in the as-needed group, standardised mean difference 0.23, 95% CI: 0.52, 1.12. REVIEWER'S CONCLUSIONS These results support current guidelines. There is little advantage in using short-acting beta2-agonists regularly, and potentially some small clinical disadvantage.
Role of bacterial and genetic factors in gastric cancer in Costa Rica.

AIM To evaluate several risk factors for gastric cancer (GC) in Costa Rican regions with contrasting GC incidence rate (GCIR). METHODS According to GCIR, 191 Helicobacter pylori (H pylori)-positive patients were classified into groups A (high GCIR, n = 101) and B (low GCIR, n = 90). Human DNA obtained from biopsy specimens was used in the determination of polymorphisms of the genes coding for interleukin (IL)-1beta and IL-10 by PCR-RFLP, and IL-1RN by PCR. H pylori DNA extractions obtained from clinical isolates of 83 patients were used for PCR-based genotyping of H pylori cagA, vacA and babA2. Human DNA from gastric biopsies of 52 GC patients was utilized for comparative purposes. RESULTS Cytokine polymorphisms showed no association with GCIR variability. However, gastric atrophy, intestinal metaplasia and strains with different vacA genotypes in the same stomach (mixed strain infection) were more frequently found in group A than in group B, and cagA and vacA s1b were significantly associated with high GCIR (P = 0.026 and 0.041, respectively). IL-1beta+3954_T/C (OR 2.1, 1.0-4.3), IL-1RN*2/L (OR 3.5, 1.7-7.3) and IL-10-592_C/A (OR 3.2, 1.5-6.8) were individually associated with GC, and a combination of these cytokine polymorphisms with H pylori vacA s1b and m1 further increased the risk (OR 7.2, 1.4-36.4). CONCLUSION Although a proinflammatory cytokine genetic profile showed an increased risk for developing GC, the characteristics of H pylori infection, in particular the status of cagA and vacA genotype distribution seemed to play a major role in GCIR variability in Costa Rica.
The Effects of Influenza A Virus PB1-F2 Protein on Polymerase Activity Are Strain Specific and Do Not Impact Pathogenesis

ABSTRACT The influenza A virus PB1-F2 protein has been implicated as a virulence factor, but the mechanism by which it enhances pathogenicity is not understood. The PB1 gene segment of the H1N1 swine-origin influenza virus pandemic strain codes for a truncated PB1-F2 protein which terminates after 11 amino acids but could acquire the full-length form by mutation or reassortment. It is therefore important to understand the function and impact of this protein. We systematically assessed the effect that PB1-F2 expression has on viral polymerase activity, accumulation and localization of PB1, and replication in vitro and in mice. We used both the laboratory strain PR8 and a set of viruses engineered to study clinically relevant PB1-F2 proteins. PB1-F2 expression had modest effects on polymerase activity, PB1 accumulation, and replication that were cell type and virus strain dependent. Disruption of the PB1-F2 reading frame in a recent, seasonal H3N2 influenza virus strain did not affect these parameters, suggesting that this is not a universal function of the protein. Disruption of PB1-F2 expression in several backgrounds or expression of PB1-F2 from the 1918 pandemic strain or a 1956 H1N1 strain had no effect on viral lung loads in mice. Alternate mechanisms besides alterations to replication are likely responsible for the enhanced virulence in mammalian hosts attributed to PB1-F2 in previous studies.
Design issues and the system architecture of TICOM-IV, a highly parallel commercial computer

We describe design issues and preliminary considerational factors for highly parallel commercial computer design. The design issues described in this paper include application area analysis, scalability, availability, network topology and memory hierarchy. We explain how these issues are related to each other and how they affect the system performance. We then describe the system architecture of TICOM-IV, a highly parallel commercial computer, and show how the design issues and the trade-offs are taken into consideration in the course of the system design. Then the architectural model is simulated in order to optimize the system performance. The simulation result shows that TICOM-IV has good performance scalability. This paper also includes some findings in the development trend for parallel computer systems from a survey of 11 commercialized parallel computers. <<ETX>>
Study of Relationship between Optimism, Pessimism and Coping Strategies with Mental Health among University Students of Lorestan

The present study is conducted to determine the relationship between optimism, pessimism and coping strategies and mental health and ability to predict these concepts in students mental health in the academic year 2014 in Lorestan University. 367 subjects sampling via stratified random were selected among students of Lorestan University. Tools for data collection in this study are mental health questionnaire (GHQ-28), Life Orientation Test-Revised (LOT-R) and a questionnaire dealing with stressful situations (CISS). The method of study is correlational and data are analyzed using the stepwise regression. Data analysis shows that there are significant relationships among optimism, pessimism, and mental health (p < 0.01). There is positive and significant relationship between problem-oriented coping strategies and subject’s mental health. Also there is a significant negative relationship between coping strategies and mental health (p < 0.01). The results of study show that optimism, pessimism, coping strategies and problem-oriented coping, differently explain variance related to mental health and its subscales significantly (p < 0.01). Generally speaking, optimistic people have more mental health because of following health lifestyles and behavior and confirming problem-oriented coping strategies. Those who follow have higher capacity of solving the problem, because they involve with the problem directly, so they have higher level of mental health.
Why should gender differences in hospitality really matter? A study of personnel’s service orientation and job satisfaction in hotels

In this article we examine the gender differences among hotel employees in service orientation and job satisfaction. To determine these differences, we used a service orientation scale (SOS), developed by Dienhart, Gregoire, Downey and Knight and a job satisfaction scale developed by Lytle. Our assumptions were that there would be significant gender differences in service orientation and job satisfaction. Our research proved factor structures of the two scales we used. Specifically, we showed gender differences in two of three factors, concerning service orientation (organisational support and customer focus), and no gender differences in job satisfaction. Our study revealed that organisational support is more important to men because they value more structured labour and service procedures than women (t = 2.21, df = 110, p = 0.03). In contrast, customer focus is more relevant to a women because it is more crucial to see satisfied guests, as well as having a good relationship with them (t = 2.07, df = 110, p = 0.04). It is of great importance for hotel management to have in mind that male and female employees respond and behave differently depending on the stimuli in the work environment and their satisfaction varies because of it.
Long‐term Results of Low‐friction Arthroplasty in Patients 30 Years of Age or Younger

The long-term results of 49 Charnley low-friction arthroplasties performed in patients 30 years of age or younger at the time of surgery was reviewed, with special emphasis placed on the radiologic evaluation of the acetabular and femoral components with average follow-up period of nearly ten years and the longest follow-up period of 15.5 years. Fifty-three percent of the sockets had no cement-bone demarcation and 28.6% had only a small line in the upper outer quadrant of the socket. Of the seven sockets that failed (14.3%), six had very deficient bone stock at operation. There were only two cases of femoral loosening. The rate of wear was 1.7 mm at ten years, which is not much greater than in older patients. Most of these cases were patients with conditions causing built-in restraints against over activity.
Morphological and molecular characterization of two new species of Andracantha (Acanthocephala: Polymorphidae) from New Zealand shags (Phalacrocoracidae) and penguins (Spheniscidae) with a key to the species

Abstract Two new species of Andracantha (Polymorphidae) are described from the intestine of the shags Leucocarbo chalconotus (Gray) and Phalacrocorax punctatus (Sparrman), and the penguin Eudyptula minor (Forster) from southern South Island, New Zealand. Andracantha leucocarboi n. sp. is distinguished from its congeners by having no genital or ventral trunk spines, but possessing a scattering of small spines between the anterior fields of spines. This is the first record of a species of Andracantha from a penguin. Circumbursal papillae are illustrated in a scanning electron micrograph for the first time in the polymorphids. Andracantha sigma n. sp. is distinguished by the sigmoid shape of its largest proboscis hook, hook VIII, and having the ventral field separated from the posterior disc field by an aspinous gap. A Maximum Likelihood tree from cox1 and large ribosomal subunit (LSU) data shows A. leucocarboi n. sp. to be more closely related to A. gravida than A. sigma n. sp. and the genus Andracantha as sister to Corynosoma spp. Genetic distances between species of Andracantha are comparatively large. A key to the species of Andracantha is provided.
Dermabrasion in Acquired Melanocytic Nevi: A Histopathological and Immunohistochemical Study

Introduction:When subjected to trauma, acquired melanocytic nevi may present atypical histological features similar to alterations that occur after exposure to ultraviolet radiation. These persist for up to 3 weeks. To date, no study has evaluated the alterations that occur after dermabrasion in acquired melanocytic nevi. Objective:To evaluate the histopathological and immunohistochemical alterations those occur in acquired melanocytic nevi, 4 weeks after dermabrasion. Materials and Methods:This study evaluated 50 acquired melanocytic nevi, in which half of each lesion was submitted to dermabrasion, leaving the other half intact, and compared the histopathological and immunohistochemical findings from the 2 sides 4 weeks after the procedure. Results:Five acquired melanocytic nevi were from male and 45 from female patients. The age of the patients varied from 15 to 69 years. On the dermabraded side, there was a greater frequency of atypical melanocytes (P = 0.015), increase in relation to nucleus/cytoplasm of the melanocytes (P = 0.034); inflammatory infiltrate (P = 0.007); and neovascularization (P = 0.004). In the immunohistochemical evaluation, there was a significantly higher percentage of immunoreactive melanocytes on this side for the HMB-45 only in the junctional component (P = 0.001) and for Ki-67, at a low rate, in the dermis (P = 0.03). Conclusions:Four weeks after the dermabrasion, atypical features, such as melanocytic atypia and pagetoid spread remained. Although these alterations may simulate melanoma in situ, the association with features of histopathological tissue repair (inflammatory infiltrate and neovascularization), together with immunohistochemical findings that are uncharacteristic of malignancy, favors a diagnosis of a benign lesion. These atypical alterations persist longer than those found after ultraviolet radiation.
Hedonic Prices and Implicit Markets: Product Differentiation in Pure Competition

A class of differentiated products is completely described by a vector of objectively measured characteristics. Observed product prices and the specific amounts of characteristics associated with each good define a set of implicit or "hedonic" prices. A theory of hedonic prices is formulated as a problem in the economics of spatial equilibrium in which the entire set of implicit prices guides both consumer and producer locational decisions in characteristics space. Buyer and seller choices, as well as the meaning and nature of market equilibrium, are analyzed. Empirical implications for hedonic price regressions and index number construction are pointed out.
An articulated handle to improve the ergonomic performance of robotic dextrous instruments for laparoscopic surgery

Hand-held robotic instruments with dextrous end-effectors offer increased accessibility and gesture precision in minimally invasive laparoscopic surgery. They combine advantages of both intuitive but large, complex , and expensive telesurgery systems, and much cheaper but less user-friendly steerable mechanical instruments. However, the ergonomics of such instruments still needs to be improved in order to decrease surgeon discomfort. Based on the results of former experimental studies, a handle connected to the instrument shaft through a lockable ball joint was designed. An experimental assessment of ergonomic and gesture performance was performed on a custom-made virtual reality simulator. Results show that this solution improves ergonomics, demanding less wrist flexion and deviation and elbow elevation, while providing gesture performance similar to a robotic dextrous instrument with standard pistol-like handle configuration.
The Emergence of Posttraumatic Distress in Later Life: A Review

We review the literature for evidence of posttraumatic stress disorder that emerges in older adulthood yet is related to earlier life events. Minimum standards necessary to establish the phenomenon are proposed and applied to existing group and case studies. Our findings suggest that the best evidence for this rests with a handful of group studies featuring male war veterans (n = 4) and a variety of case studies (n = 12) that have appeared over the last two decades. We summarize what is known regarding prevalence, symptoms, course, and triggers, and conclude that the phenomenon does exist but has rarely been investigated systematically. There are therefore no grounds at present for thinking of reemergent posttraumatic stress disorder as a unique subtype of the disorder among elderly people. We suggest that future research focuses in more detail on participants' history of posttraumatic difficulties and considers the phenomenon across groups other than male veterans.