Miracle-dz/UniFAR-FTUs huggingface dataset:
Silent and continuous fMRI scanning differentially modulate activation in an auditory language comprehension task

Sparse temporal acquisition schemes have been adopted to investigate the neural correlates of human audition using blood‐oxygen‐level dependent (BOLD) based functional magnetic resonance imaging (fMRI) devoid of ambient confounding acoustic scanner noise. These schemes have previously been extended to clustered‐sparse temporal acquisition designs which record several subsequent BOLD contrast images in rapid succession in order to enhance temporal sampling efficiency. In the present study we demonstrate that an event‐related task design can effectively be combined with a clustered temporal acquisition technique in an auditory language comprehension task. The same fifteen volunteers performed two separate auditory runs which either applied customary fMRI acquisition (CA) composed of continuous scanner noise or “silent” fMRI built on a clustered temporal acquisition (CTA) protocol. In accord with our hypothesis, the CTA scheme relative to the CA protocol is accompanied by significantly stronger functional responses along the entire superior temporal plane. By contrast, the bilateral insulae engage more strongly during continuous scanning. A post‐hoc region‐of‐interest analysis reveals cortical activation in subportions of the supratemporal plane which varies as a function of acquisition protocol. The middle part of the supratemporal plane shows a rightward asymmetry only for the CTA scheme while the posterior supratemporal plane exposes a significantly stronger leftward asymmetry during the CTA. Our findings implicate that silent fMRI is advantageous when it comes to the exploration of auditory and speech functions residing in the supratemporal plane. Hum Brain Mapp, 2008. © 2007 Wiley‐Liss, Inc.
Chemokines define distinct microenvironments in the developing thymus

During thymus development, prothymocytes home to the thymus where they migrate as maturing thymocytes from the cortex to the medulla. Chemotaxis assays show that developing T cells of newborn mice respond to certain chemokines depending on their differentiation state. In situ expression analyses indicate that the same chemokines are expressed in distinct microenvironments within the thymic stroma. Expression of chemokines is regulated temporally during embryogenesis; in the alymphoid early thymic anlage, only TECK, SDF‐1 and SLC but not ELC, MDC or TARC are expressed. Fetal blood prothymocytes destined to colonize the thymus respond to the embryonic chemokines TECK and SDF‐1 in chemotaxis assays with high efficacy. The in vivo significance of this finding is demonstrated by studies in the nude mouse where the thymic anlage lacks TECK and SDF‐1 expression and prothymocytes home to the parathyroid anlage rather than to the thymic anlage. Developing thymocytes respond to chemokines expressed in distinct microenvironments within the thymic stroma in a way that correlates well with the previously observed migration pattern from cortex to medulla. The complexity of these chemokine‐defined microenvironments increases as the thymic anlage develops to a mature thymus.
Influence of human resources to the effect of system quality and information quality on the user satisfaction of accrual-based accounting system

The purpose of this study is to examine the effect of system quality and information quality on the User satisfaction of The Accrual-Based Accounting System of Institutions application moderated by Human Resources Quality in service partner work units of the Indonesia. The population of this study consisted of 209 work units. The sampling was conducted by using sensus method using the primary data collected through questionnaires. The data were then analyzed by using the Structural Equation Modelling-Partial Least Squares. The results showed that the quality of system, information, and human resources had a significant positive effect on the User satisfaction of Accrual-Based Accounting System of Institutions application. The Human Resources quality as the moderating variable was able to moderate the relationship between the system quality and information quality in the User satisfaction of Accrual-Based Accounting System of Institutions application.
Intermediate term biochemical-free progression and local control following 125iodine brachytherapy for prostate cancer.

PURPOSE We determined the 10-year biochemical and local control results for I prostate brachytherapy in men followed a minimum of 4 years. MATERIALS AND METHODS A total of 279 men with T1-T2 prostate cancer with a minimum followup of 4 years were implanted with I from 1990 to 1998 using the real-time technique. Patients were treated with the implant alone (215 or 72.5%) or with the implant and 6 months of hormone therapy (64 or 27.6%). Of the men 185 (66.3%) agreed to ultrasound guided biopsy (6 to 12 cores) a minimum of 2 years after implantation. All patients with increasing prostate specific antigen (PSA), evidence of local recurrence or a prior positive biopsy underwent repeat biopsy yearly until biopsy became negative or there was clear evidence of biochemical (PSA) progression. The radiation dose delivered to 90% of the gland (D90) was determined 30 days after implantation by computerized tomography based dosimetry. Biochemical failure was defined as 3 consecutive PSA increases. Survival curves were calculated by the Kaplan-Meier method. Cross tabulations were tested by Pearson chi-square analysis. The effect of multiple variables was tested by the log rank test (Cox regression). RESULTS Median patient age was 67 years (range 42 to 82) and median followup was 6 years (range 4 to 12). Of the patients 49 (17.6%) experienced failure, for a 10-year freedom from failure (FFF) rate of 78%. Univariate analysis for 10-year FFF demonstrated that initial PSA (p = 0.001), stage (p = 0.002), risk group (p <0.001), hormone therapy (p = 0.013) and D90 (p <0.001) were significant. Multivariate analysis demonstrated that D90 (p <0.001) and risk group (p = 0.013) were the only significant variables. The RR of PSA failure was 3.0 (95% CI 2.0 to 4.4, p <0.001) and 5.6 (95% CI 3.1 to 10, p <0.001) for doses below 140 and 120 Gy, respectively. Of the 185 patients 166 (90%) had a negative post-implantation prostate biopsy. FFF was 85% vs 21% in those with a positive biopsy (p <0.001). Patients with a D90 of at least 140 Gy had a positive biopsy rate of 4.8% compared to 20.5% in those with a lower dose (p <0.001). The RR for positive biopsy at doses less than 140 and 120 Gy was 2.6 (95% CI 1.6 to 4.4, p = 0.002) and 4.3 (95% CI 2.3 to 8.1, p <0.001), respectively. CONCLUSIONS These data demonstrate high biochemical and local control in men with T1-T2 prostate cancer treated with I brachytherapy. The delivered radiation dose and risk category are important predictors of success. Patients receiving a dose of at least 140 Gy have a 90% chance of biochemical FFF and a 95.2% likelihood of local control.
Stabilization of myelin mRNAs as measured in a brain slice system

The stabilization and destabilization of myelin mRNA is increasingly recognized as a major control point in regulating myelin gene expression. A brain slice system was developed and characterized to study mRNA stability in actively myelinating oligodendrocytes. The mRNA half‐life of a major CNS myelin protein, proteolipid protein (PLP), was measured to be 5 hr. The half‐life of another CNS myelin protein mRNA, myelin basic protein (MBP), was measured to be greater than 12 hr. A long half‐life for MBP mRNA is consistent with MBP mRNA being stable long enough to be translocated to the myelin internode where it is then translated. Using semi‐quantitative reverse transcriptase‐PCR, it was determined that there was no differential stabilization between the two major PLP mRNA isoforms, PLP and DM20. It was also determined that protein synthesis was required for the specific stabilization of PLP/DM20 mRNAs. Inasmuch as PLP is a major structural protein of the CNS myelin, the PLP/DM20 mRNAs have relatively short half‐lives. However, the PLP/DM20 mRNAs half‐lives may be increased by the action of trans‐acting factors that are themselves very labile. J. Neurosci. Res. 50:1030–1039, 1997. © 1997 Wiley‐Liss, Inc.
Effects of pioglitazone versus glimepiride exposure on hepatocellular fat content in type 2 diabetes

Thiazoledinediones decrease blood glucose by their insulin‐sensitizing properties. Here, we examined whether pioglitazone plus nateglinide (PIO) interferes with hepatocellular lipid (HCL) content and/or improves insulin sensitivity in well‐controlled non‐obese patients with type 2 diabetes mellitus (T2DM).
Puberty: A Finishing School for Male Social Behavior

Abstract: The classical view of steroid‐dependent organization of brain and behavior holds that gonadal steroid hormones, acting during an early critical period of development, cause permanent structural changes in neural circuits that determine behavioral responses to hormones in adulthood. This classical view has been modified to incorporate evidence that organizational effects of steroids can occur outside of the established perinatal critical period and that multiple critical periods may exist during development. Experiments in this laboratory indicate that steroid‐dependent organization of neural circuits underlying male social behaviors occurs during puberty. This work shows that adult‐typical reproductive and flank marking behaviors cannot be activated by gonadal steroids in male Syrian hamsters prior to puberty, suggesting that developmentally timed processes during puberty render the nervous system responsive to activating effects of gonadal steroids in adulthood. Additional experiments demonstrate that the presence or absence of gonadal hormones during puberty is a major factor in the ability of steroids to activate reproductive and flank marking behavior in adult male hamsters and in androgen receptor expression within the neural circuit underlying these behaviors. Thus, gonadal hormones during puberty appear to exert long‐lasting changes in neural circuits that are responsible for the programming of activational responses to steroids later in adulthood. A two‐stage model for maturation of male social behaviors is proposed: a perinatal critical period for sexual differentiation of neural circuits, followed by the pubertal period, during which gonadal steroids further organize the circuits to enhance behavioral responsiveness to hormones in adulthood. Whether puberty is a critical period for the proposed second wave of steroid‐dependent organization of behavioral circuits remains to be determined.
Memory Efficient Scheduling for Multicore Real-time Systems

Modern real-time systems are becoming increasingly complex and requiring significant computational power to meet their demands. Since the increase in uniprocessor speed has slowed down in the last decade, multicore processors are now the preferred way to supply the increased performance demand of real-time systems. A significant amount of work in the real-time community has focused on scheduling solutions for multicore processors for both sequential and parallel real-time tasks. Even though such solutions are able to provide strict timing guarantees on the overall response time of real-time tasks, they rely on the assumption that the worst-case execution time (WCET) of each individual task is known. However, physical shared resources such as main memory and I/O are heavily employed in multicore processors. These resources are limited and therefore subject to contention. In fact, the execution time of one task when run in parallel with other tasks is significantly larger than the execution time of the same task when run in isolation. In addition, the presence of shared resources increases the timing unpredictability due to the conflicts generated by multiple cores. As a result, the adoption of multicore processors for real-time systems is dependent upon solving such sources of unpredictability. In this dissertation, we investigate memory bus contention. In particular, two main problems are associated with memory contention: (1) unpredictable behavior and (2) hindrance of performance. We show how to mitigate these two problems through scheduling. Scheduling is an attractive tool that can be easily integrated into the system without the need for hardware modifications. We adopt an execution model that exposes memory as a resource to the scheduling algorithm. Thus, the theory of real-time multiprocessor scheduling, that has seen significant advances in recent years, can be utilized to schedule both processor cores and memory. Since the real-time workload on multicore processors can be modeled as sequential or parallel tasks, we also study parallel task scheduling by taking memory time into account.
Flow cytometry using the monoclonal antibody CD10‐Pe/Cy5 is a useful tool to identify follicular lymphoma cells

Abstract: Follicular lymphoma (FL) is a specific entity defined by characteristic histology, phenotype and molecular rearrangements. Classically, reactivity for CD19, CD10, and strong positivity for the surface light chain immunoglobulin (SIg) are considered to be phenotypic signs typically expressed in FL. In practice, this pattern is difficult to identify since most neoplastic cells analysed by flow cytometry (FC) show weak intensity for CD19‐Pe/Cy5 and for SIg and negativity for CD10‐FITC. We used triple antigen combinations including two monoclonal antibodies (MoAbs) against CD10 (CD10‐FITC and CD10‐Pe/Cy5) and a long‐distance polymerase chain reaction (PCR) approach to establish the phenotypic pattern of neoplastic cells carrying t(14;18)(q32;q21). Neoplastic cells showed the following immunophenotype: stronger reactivity against CD20 than against CD19, positivity for CD22 and SIg and negativity for CD5, CD11c and CD10‐FITC. Characteristically, CD10‐Pe/Cy5 was expressed in all the samples with positive bcl‐2/JH rearrangements. In FL, there was a high correlation between histologic diagnosis and reactivity against CD10‐Pe/Cy5 (96% cases). In diffuse large cell lymphomas (DLCL), CD10‐Pe/Cy5 identified positive cases with t(14;18)(q32;q21) chromosomal translocation, whereas Burkitt lymphomas showed all cases reactivity against CD10‐Pe/Cy5. In conclusion, CD10‐Pe/Cy5 is a useful antibody for identifying neoplastic cells carrying t(14;18)(q32;q21) in FL and DLCL. In combination with other MoAbs, anti‐CD10 (HI10a, Cy‐Chrome) can be used to identify a characteristic phenotypic profile of FL against other lymphoproliferative disorders.
Patients' profile, clinical presentations and histopathological features of colo-rectal cancer in Al Hassa region, Saudi Arabia.

BACKGROUND Patients' demographics, presenting clinical and histopathological features for colo-rectal cancer (CRC) are important factors for patients' outcome and disease prognosis. This study aimed to describe the pattern of CRC in terms of patients' demographics, main presenting symptoms and histopathological features in Al Hassa region of Saudi Arabia. SUBJECTS AND METHODS A retrospective hospital records-based study which included reviewing of patients' records diagnosed with CRC at three general hospitals in Al Hassa region, Saudi Arabia. A compilation form was designed to collect information regarding socio-demographics, age at diagnosis; referral sites and the main presentations at CRC diagnosis. sites and the main presentations at CRC diagnosis. Histopathological reports were reviewed to delineate the main cytopathologic features, prominent cytological characteristics, the presence of predisposing pathology, and disease stage. RESULTS Of the 142 cases with CRC, 15.5% and 33.1% were affected before 40 and 50 years of age respectively. Emergency rooms were main referral sites for CRC cases (31.0%). Right colon was affected in 16.9% while left colonic lesions accounted for 62.7%. Intestinal obstruction was the main presentations (41.5%), and 26.1% presented with symptoms indicating distant metastatic lesions. Adenocarcinoma was the predominant pathological lesions (86.6%). Metastatic CRC was diagnosed in 62.7%. Duke's staging showed that 22.5% and 40.1% of lesions were classified into C and D categories respectively. CONCLUSION Saudi patients with CRC present late with distant metastasis, and advanced disease stage. A sizeable proportion of patients developed the lesions at relatively young age. Screening recommendations should be enacted and revised in response to current change with younger age of affection.
Pharmacodynamics of Glycopeptides in the Mouse Peritonitis Model of Streptococcus pneumoniae orStaphylococcus aureus Infection

ABSTRACT The emergence of resistance to various antibiotics in pneumococci leaves the glycopeptides as the only antibiotics against which pneumococci have no resistance mechanism. This situation has led to a renewed interest in the use of glycopeptides. It has not yet been possible to conclude which one or more of the pharmacokinetic or pharmacodynamic (PK/PD) parameters are the most important and best predictors for the effects of treatment with glycopeptides in animal models or in humans. We used the mouse peritonitis model with immunocompetent mice and with Staphylococcus aureus and Streptococcus pneumoniae as infective organisms. A wide spectrum of different treatment regimens with vancomycin and teicoplanin was tested to study the pharmacodynamics of these drugs. In studies in which the single dose that protected 50% of lethally infected mice (ED50) was given as one dose or was divided into two doses, survival was significantly decreased when the dose was divided. The only statistically significant correlations between the percentage of survival of the mice after 6 days and each of the PK/PD parameters were for peak concentration (Cmax)/MIC and S. aureus and for the free fraction of Cmax(Cmax-free)/MIC and S. pneumoniae. For S. pneumoniae, the ED50 for different dosing regimens increased with the number of doses given; e.g., the single-dose ED50s for vancomycin and teicoplanin were 0.65 and 0.45 mg/kg, respectively, but the ED50s for dosing regimens with 2-h doses given for 48 h were 6.79 and 5.67 mg/kg, respectively. In experiments with 39 different vancomycin dosing regimens and 40 different teicoplanin dosing regimens against S. pneumoniae, the different PK/PD parameters were analyzed using logistic regression. The Cmax-free/MIC was one of two parameters that best explained the effect for both drugs; for vancomycin, the other important parameter was the AUC/MIC, and for teicoplanin, the other parameter was the time the free fraction of the drug is above the MIC. The effect analyzed as a function ofCmax-free/MIC disclosed thresholds with shifts from almost no effect to full effect at ratios of five to six for vancomycin and two to three for teicoplanin.
Single-sample method for the estimation of glomerular filtration rate in children.

A method for the determination of the glomerular filtration rate (GFR) in children which involves the use of a single-plasma sample (SPS) after the injection of a radioactive indicator such as radioiodine labeled diatrizoate (Hypaque) has been developed. This is analogous to previously published SPS techniques of effective renal plasma flow (ERPF) in adults and children and GFR SPS techniques in adults. As a reference standard, GFR has been calculated from compartment analysis of injected radiopharmaceuticals (Sapirstein Method). Theoretical volumes of distribution were calculated at various times after injection (Vt) by dividing the total injected counts (I) by the plasma concentration (Ct) expressed in liters, determined by counting an aliquot of plasma in a well type scintillation counter. Errors of predicting GFR from the various Vt values were determined as the standard error of estimate (Sy.x) in ml/min. They were found to be relatively high early after injection and to fall to a nadir of 3.9 ml/min at 91 min. The Sy.x Vt relationship was examined in linear, quadratic, and exponential form, but the simpler linear relationship was found to yield the lowest error. Other data calculated from the compartment analysis of the reference plasma disappearance curves are presented, but at this time have apparently little clinical relevance.
Characterization of spermidine binding to solubilized plasma membrane proteins from zucchini hypocotyls

In this work [14C]spermidine binding to total proteins solubilized from plasma membrane purified from zucchini (Cucurbita pepo L.) hypocotyls was investigated. Proteins were solubilized using octyl glucoside as a detergent. Specific polyamine binding was thermolabile, reversible, pH dependent with an optimum at pH 8.0, and had a Kd value of 5 &mgr;M, as determined by glass-fiber-filter assays. Sephadex G-25 M gel-filtration assays confirmed the presence of a spermidine-protein(s) complex with a specific binding activity. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis of collected fractions having the highest specific spermidine-binding activity, several protein bands (113, 75, 66, and 44 kD) were identified. The specificity of spermidine binding was examined by gel-filtration competition experiments performed using other polyamines and compounds structurally related to spermidine. Partial purification on Sephadex G-200 led to the identification of 66- and 44-kD protein bands, which may represent the putative spermidine-binding protein(s) on the plasmalemma.
Glued Intrascleral Fixation of Intraocular Lens With Pupilloplasty and Pre-Descemet Endothelial Keratoplasty: A Triple Procedure

Purpose: To describe a surgical approach of glue-assisted intrascleral fixation of an intraocular lens (IOL) with pupilloplasty and pre-Descemet endothelial keratoplasty as a single-stage procedure. Methods: Glue-assisted intrascleral fixation of an IOL is performed initially because it helps to secure the IOL fixation, followed by pupilloplasty that imparts stability to the anterior chamber and prevents air diversion into the vitreous cavity, thereby facilitating the pre-Descemet endothelial keratoplasty procedure that is performed to complete the surgical repair. Results: The procedure was performed in 5 eyes of 5 patients, and the donor age ranged from 9 months to 65 years. There was a significant change in the uncorrected (P = 0.034) and corrected (P = 0.043) distance visual acuities in the postoperative period. The mean graft size was 7.6 ± 0.4 mm (range, 7–8 mm). The mean preoperative and postoperative specular endothelial counts were 2788 ± 204 cells per square millimeter and 1898 ± 90 cells per square millimeter, respectively. No incidence of primary graft failure or graft rejection during the entire follow-up period was reported in any of the eyes. Conclusions: The combined procedure serves as an effective method in select cases of endothelial decompensation that require a secondary IOL implantation or an IOL exchange with good postoperative results.
The regulated elimination of transit-amplifying cells preserves tissue homeostasis during protein starvation in Drosophila testis

How tissues adapt to varying nutrient conditions is of fundamental importance for robust tissue homeostasis throughout the life of an organism, but the underlying mechanisms are poorly understood. Here, we show that Drosophila testis responds to protein starvation by eliminating transit-amplifying spermatogonia (SG) while maintaining a reduced pool of actively proliferating germline stem cells (GSCs). During protein starvation, SG die in a manner that is mediated by the apoptosis of somatic cyst cells (CCs) that encapsulate SG and regulate their development. Strikingly, GSCs cannot be maintained during protein starvation when CC-mediated SG death is inhibited, leading to an irreversible collapse of tissue homeostasis. We propose that the regulated elimination of transit-amplifying cells is essential to preserve stem cell function and tissue homeostasis during protein starvation. SUMMARY: The Drosophila testis responds to protein starvation by eliminating transit-amplifying spermatogonia while maintaining actively proliferating germline stem cells, revealing a tissue-wide response that maintains homeostasis.