Miracle-dz/UniFAR-FTUs huggingface dataset:
Barium triggers rapid endocytosis in calf adrenal chromaffin cells

1 Changes in cell capacitance were monitored in whole‐cell patch‐clamp recordings from calf adrenal chromaffin cells using a software‐based phase‐tracking technique. Rapid endocytosis and exocytosis were observed in extracellular solutions containing either Ca2+ or Ba2+. 2 There was no significant difference in the magnitude or the time course of rapid endocytosis of cells stimulated in Ca2+ as compared to Ba2+. When cells were pretreated with caffeine and thapsigargin in order to deplete intracellular Ca2+ stores, rapid endocytosis in Ba2+ was not affected. This indicates that Ba2+ itself is capable of supporting rapid endocytosis. 3 The application of the calmodulin inhibitor calmidazolium via the intracellular pipette solution did not inhibit rapid endocytosis. Although our findings are inconsistent with an immediate requirement for calmodulin in rapid endocytosis, they do not rule out an involvement on a longer time scale. 4 While rapid endocytosis was not affected by the substitution of Ca2+ with Ba2+, the maximum rate of exocytosis was higher in cells stimulated in Ca2+ than in Ba2+. Since Ba2+ currents were much larger than Ca2+ currents during depolarizations to +10 mV (the test potential used in these experiments), Ba2+ appears to be less efficient at promoting exocytosis than Ca2+.
99mTc-Phytate Lymphoscintigraphy for Detection of Sentinel Node: Preliminary Results of the First Year's Clinical Experience in Isfahan, Iran

Sentinel lymph node is the first regional lymph node that drains the lymph from the primary tumor. It is potentially the first node to receive the seeding of lymph-borne metastatic cells. This study aimed to discuss lymphoscintigraphy procedural guidelines for detection of sentinel node using 99mTc-Phytate in Isfahan, Iran. Moreover, the preliminary results of the first year′s clinical experience of lymphoscintigraphy in Isfahan, Iran are also presented. A total of 36 consecutive sentinel node procedures were performed following our protocol in March 2013 to March 2014. For all 36 patients, after intradermal injection of 0.5-1 mCi of 99mTc-Phytate, 5, 30 and 120 min with hands up lymphoscintigraphy was performed. All procedures were performed in a 1-day setting with 99mTc-Phytate injection in intradermal volume of about 0.1 cc. At 5, 30 and 120 min after injection, anterior and lateral images (4 min), were acquired using gamma-camera (energy 140 keV, window 15-20% and LEHR collimator). For all patients, at least one axillary sentinel lymph node was detected. For three patients, 2 SNs were seen. The images 5 min after injection showed at least one axillary sentinel node in 18 of 36 patients. However for the remaining patients, more delayed images (after 30 and 120 min) were needed. Although, no changes were seen in 120 min images compared to 30 min images. Considering the used protocol, from the evaluated data it can be concluded that lymphoscintigraphy after 30 min periareolar injection of about 0.5-1 mCi 99mTc-Phytate in an intradermal volume of about 0.1 cc yields an axillary sentinel node in all the patients. Imaging 120 min after injection is of no additional value and can be omitted.
Optimal release time for software systems considering cost, testing-effort, and test efficiency

In this paper, we study the impact of software testing effort & efficiency on the modeling of software reliability, including the cost for optimal release time. This paper presents two important issues in software reliability modeling & software reliability economics: testing effort, and efficiency. First, we propose a generalized logistic testing-effort function that enjoys the advantage of relating work profile more directly to the natural flow of software development, and can be used to describe the possible testing-effort patterns. Furthermore, we incorporate the generalized logistic testing-effort function into software reliability modeling, and evaluate its fault-prediction capability through several numerical experiments based on real data. Secondly, we address the effects of new testing techniques or tools for increasing the efficiency of software testing. Based on the proposed software reliability model, we present a software cost model to reflect the effectiveness of introducing new technologies. Numerical examples & related data analyzes are presented in detail. From the experimental results, we obtain a software economic policy which provides a comprehensive analysis of software based on cost & test efficiency. Moreover, the policy can also help project managers determine when to stop testing for market release at the right time.
How Successful are Highly Qualified Return Migrants in the Lithuanian Labour Market

This article analyses whether the human capital gained abroad helps returning migrants to integrate into the Lithuanian labour market. The analysis focuses on highly qualified migrants, defined narrowly as people with higher education who held qualified jobs when working abroad. The article found that for this group of returnees professional aims were important when taking a decision to return, together with other motives such as family reasons and home-sickness. Most of the returnees were able to find employment and pursue their careers without major difficulties, although a significant minority signalled an intention to emigrate again. The article examines the key factors that sometimes helped and sometimes hindered integration of the returnees and discusses the role of the public policy.
MYO1E mutations and childhood familial focal segmental glomerulosclerosis.

BACKGROUND Focal segmental glomerulosclerosis is a kidney disease that is manifested as the nephrotic syndrome. It is often resistant to glucocorticoid therapy and progresses to end-stage renal disease in 50 to 70% of patients. Genetic studies have shown that familial focal segmental glomerulosclerosis is a disease of the podocytes, which are major components of the glomerular filtration barrier. However, the molecular cause in over half the cases of primary focal segmental glomerulosclerosis is unknown, and effective treatments have been elusive. METHODS We performed whole-genome linkage analysis followed by high-throughput sequencing of the positive-linkage area in a family with autosomal recessive focal segmental glomerulosclerosis (index family) and sequenced a newly discovered gene in 52 unrelated patients with focal segmental glomerulosclerosis. Immunohistochemical studies were performed on human kidney-biopsy specimens and cultured podocytes. Expression studies in vitro were performed to characterize the functional consequences of the mutations identified. RESULTS We identified two mutations (A159P and Y695X) in MYO1E, which encodes a nonmuscle class I myosin, myosin 1E (Myo1E). The mutations in MYO1E segregated with focal segmental glomerulosclerosis in two independent pedigrees (the index family and Family 2). Patients were homozygous for the mutations and did not have a response to glucocorticoid therapy. Electron microscopy showed thickening and disorganization of the glomerular basement membrane. Normal expression of Myo1E was documented in control human kidney-biopsy specimens in vivo and in glomerular podocytes in vitro. Transfection studies revealed abnormal subcellular localization and function of the A159P-Myo1E mutant. The Y695X mutation causes loss of calmodulin binding and of the tail domains of Myo1E. CONCLUSIONS MYO1E mutations are associated with childhood-onset, glucocorticoid-resistant focal segmental glomerulosclerosis. Our data provide evidence of a role of Myo1E in podocyte function and the consequent integrity of the glomerular filtration barrier.
Measurement of Risk and Protective Factors for Drug Use and Anti-Social Behavior among High School Students in South Africa

The study sought to examine, for South African adolescents: 1) the reliability of sub-scales of the Communities that Care Youth Survey (CTC Youth Survey) of risk and protective factors for drug use and anti-social behavior; and 2) the extent to which tobacco, alcohol and marijuana use can be predicted from community, family, school, and peer-individual factors based on sub-scales of the CTC Youth Survey. On two occasions, 92 male and 31 female, Grade 8 and 11 students completed measures concerning: 1) their past month tobacco, alcohol and marijuana use; and 2) various community, family, school, and peer-individual factors. Cronbach alpha coefficients of sub-scales of the questionnaire ranged between . 60 and . 94. Kappa values were at least moderate (above .40) on 19 sub-scales, and on the remaining sub-scales observed agreement levels ranged between . 49 and . 94. Each domain predicted tobacco, alcohol, and marijuana use. Multiple logistic regression analyses revealed that alcohol use was most strongly accounted for by the peer domain, tobacco use by the school domain, and marijuana use by the peer and community domains. The findings support use of the CTC Youth Survey, with slight revisions, among South African high school students.
IS THERE A DIFFERENTIAL IMPACT OF FATIGUE AND PHYSICAL DISABILITY ON QUALITY OF LIFE IN MULTIPLE SCLEROSIS?

To investigate the quantitative impact of fatigue on health-related quality of life (HRQoL) in multiple sclerosis (MS) and to determine whether fatigue was related to HRQoL independently from bodily disability, data on HRQoL were ascertained for 87 patients with definite MS by using the SF-36. HRQoL scores and subscores were related to the basic MS disability score (EDSS) and further MS parameters, and to fatigue, which was assessed by using different fatigue scales. Factors related to predominantly physical but not mental HRQoL aspects were identified as related to EDSS, duration of disease, and age. Different fatigue scores did impact significantly on both physical and especially mental HRQoL. The influence of fatigue on physical HRQoL was independent from EDSS. Fatigue experience reduces HRQoL markedly and independently from EDSS. Therefore, fatigue assessment provides additional information to disability-derived scales such as the EDSS with relevant implications for therapeutic decisions.
Advances in Treatment of Bipolar Disorders.

We may not be able to make you love reading, but advances in treatment of bipolar disorders will lead you to love reading starting from now. Book is the window to open the new world. The world that you want is in the better stage and level. World will always guide you to even the prestige stage of the life. You know, this is some of how reading will give you the kindness. In this case, more books you read more knowledge you know, but it can mean also the bore is full.
Weak‐ and strong‐friction limits of parcel models: Comparisons and stochastic convective initiation time

For moist convection, models of individual parcel dynamics are valuable for their simple formulation and predictions of cloud properties. Here, two limiting idealized cases of parcel theory are investigated: the weak‐ and strong‐friction limits. The weak‐friction limit is a traditional limit with no momentum drag and the dynamics are a Hamiltonian system for the parcel's height and vertical velocity. A strong‐friction limit is derived and studied here and its limiting form involves a balance between frictional drag and buoyancy, which provides a differential equation for parcel height as a function of time. In the two limiting regimes, analytical formulas are presented and compared for quantities such as maximum vertical velocity and cloud‐top height. For example, in the strong‐friction limit, the cloud‐top height coincides with the level of neutral buoyancy (LNB), whereas in the weak‐friction limit the cloud‐top height is far above the LNB. This comparison suggests that the strong‐friction limit may provide more realistic predictions of some averaged cloud properties. In general, since frictional effects and individual parcel properties can vary even within a single cloud, the predictions of the weak‐ and strong‐friction limits can be viewed as upper and lower bounds for the behavior of more realistic finite‐friction scenarios. Finally, in a stochastic version of the parcel model, in the strong‐friction limit, analytical formulas are derived for convective initiation time. Applications to convective parametrizations are discussed; for example, the formulas for convective initiation time could be applied as stochastic convective triggers.
Targeting Rac1 by the Yersinia Effector Protein YopE Inhibits Caspase-1-mediated Maturation and Release of Interleukin-1β*

Yersinia bacteria can take control of the host cell by injecting so-called Yop effector proteins into the cytosol of the cells to which they adhere. Using Yersinia enterocolitica strains that are deficient for one or more Yops, we could show that YopE and, to a lesser extent, YopT interfere with the caspase-1-mediated maturation of prointerleukin-1β in macrophages. In addition, overexpression of YopE and YopT was shown to prevent the autoproteolytic activation of caspase-1 in a way that is dependent on their inhibitory effect on Rho GTPases. Expression of constitutive-active or dominant-negative Rho GTPase mutants or treatment with Rho GTPase inhibitors confirmed the role of Rho GTPases and, in particular, Rac1 in the autoactivation of caspase-1. Rac1-induced caspase-1 activation was mediated by its effect on LIM kinase-1, which is targeting the actin cytoskeleton. Rac-1 and LIM kinase-1 dominant-negative mutants were shown to inhibit caspase-1 activation induced by overexpression of Asc, which is a caspase-1-activating adaptor protein. Moreover, Rac1 as well as YopE and YopT significantly modulated caspase-1 oligomerization. These results highlight a previously unknown function of Rho GTPases in the activation of caspase-1 and give new insight on the role of YopE in immune-escape mechanisms of Yersinia.
A longitudinal neuropsychological study of partial brain radiation in adults with brain tumors

Objective: To investigate longitudinal cognitive functioning in patients with brain tumor treated with modern highly conformal fractionated partial brain radiation therapy (RT). Methods: Seventeen (of 22 initial consecutive patients) adults with primarily low-grade brain neoplasms who underwent either biopsy or tumor resection were tested at pre-RT baseline and at 3, 6, 12, and 24 months after baseline. Participants were classified as RT-treated nonprogressors (n = 12) or progressors (n = 3) based on serial follow-up structural imaging. Two patients received surgery only and served as controls to help minimize surgical, practice, test form, or other potential non-RT effects. Serial neuropsychological assessments were conducted using alternate forms of the Selective Reminding Test, 10/36 Spatial Recall Test, and Symbol Digit Modality Test (oral, written) as well as the Shipley Scale (baseline only), Wechsler Adult Intelligence Scale–Revised Digit Span, Trail Making Test, and the Symptom Checklist-90–Revised Global Severity Index scale. Results: There was evidence of subtle attention and memory improvement in RT-treated nonprogressors throughout the 2-year period, with no evidence of cognitive decline. In contrast, patients with disease progression evidenced more substantial decline in memory and attention. Conclusions: Partial brain fractionated RT was not associated with adverse neuropsychological effects through the first 2 years following therapy.
Linear Characterization of Optical Pulses With Durations Ranging From the Picosecond to the Nanosecond Regime Using Ultrafast Photonic Differentiation

In this paper, we extend the recently introduced linear technique for temporal phase reconstruction using optical ultrafast differentiation (PROUD) to achieve full characterization of ultrashort optical pulses with durations down to the picosecond regime using a well-characterized temporal stretcher (e.g., dispersive optical fiber). The proposed method is experimentally demonstrated by precisely characterizing the amplitude and phase temporal profiles of microwatt-power picosecond pulses ranging from 4 to 20 ps with both continuous and discrete temporal phase variations. Using this simple mechanism, the same PROUD setup can be used to characterize optical pulses with durations ranging from the picosecond to the nanosecond regime. We provide a comprehensive mathematical analysis of this general PROUD technique: we evaluate in detail the influence of the key specifications (e.g., different sources of noise) of the used components and instruments, namely, optical differentiator, linear temporal stretcher, and time-domain intensity test equipment, on the performance of the PROUD measurement system, particularly in terms of phase sensitivity in the optical pulse characterization.
Single dose oral diclofenac for acute postoperative pain in adults.

BACKGROUND Diclofenac is a non-steroidal anti-inflammatory drug (NSAID), available as a potassium salt (immediate-release) or sodium salt (delayed-release). This review updates an earlier review published in The Cochrane Database of Systematic Reviews (Issue 2, 2004) on 'Single dose oral diclofenac for postoperative pain'. OBJECTIVES To assess single dose oral diclofenac for the treatment of acute postoperative pain. SEARCH STRATEGY Cochrane CENTRAL, MEDLINE, EMBASE, Biological Abstracts, the Oxford Pain Relief Database, and reference lists of articles were searched; last search December 2008. SELECTION CRITERIA Randomised, double-blind, placebo-controlled clinical trials of single dose, oral diclofenac (sodium or potassium) for acute postoperative pain in adults. DATA COLLECTION AND ANALYSIS Two review authors independently assessed studies for inclusion and quality, and extracted data. The area under the pain relief versus time curve was used to derive the proportion of participants with at least 50% pain relief over 4 to 6 hours, using validated equations. Relative benefit (risk) and number needed to treat to benefit (NNT) were calculated. Information on adverse events, time to remedication, and participants needing additional analgesia was also collected. MAIN RESULTS Fifteen studies (eight additional studies) with 1512 participants more than doubled the information available at each dose. Overall 50% to 60% of participants experienced at least 50% pain relief over 4 to 6 hours at any dose with diclofenac, compared to 10 to 20% with placebo, giving NNTs of about 2.5 for doses of 25 mg to 100 mg (similar to earlier review); no dose response was demonstrated. At 50 mg and 100 mg, NNTs for diclofenac potassium (2.1 (1.8 to 2.4) and 1.9 (1.7 to 2.2)) were significantly lower (better) than for diclofenac sodium (6.7 (4.2 to 17) and 4.5 (3.2 to 7.7)). The median time to use of rescue medication was 2 hours for placebo, 4.3 hours for diclofenac 50 mg and 4.9 hours for diclofenac 100 mg. Adverse events were reported at a similar rate to placebo, with no serious events. AUTHORS' CONCLUSIONS Oral diclofenac is an effective single-dose treatment for moderate to severe postoperative pain. Significantly more participants experienced at least 50% pain relief over 4 to 6 hours with diclofenac potassium than with diclofenac sodium. There was no significant difference between diclofenac and placebo in the incidence of adverse events.
Lipid Extracted Bank Bone: Bone Conductive and Mechanical Properties

Lipid extraction by chloroform methanol previously has been found to increase the incorporation of frozen bone allografts. This effect may be because of a decreased immunologic response. In the present study, the ingrowth capacity into a grafted bone defect was investigated by using the bone harvest chamber model in rabbits. In a series of experiments, defatted and frozen allografts were compared at 1, 2, and 3 weeks; defatted allografts and defatted autografts were compared at 3 weeks; and frozen or defatted allografts and nongrafted defects were compared at 3 weeks. Evaluation was performed through histology, histomorphometry, and 99mTc-MDP scintimetry. The incorporation was better with defatted grafts: by histology at 1 week, mesenchymal tissue filled the intertrabecular space in defatted specimens and new bone formation started to occur. In contrast, frozen specimens showed a central soft tissue necrosis surrounded by inflammatory cells. Histomorphometry showed more new bone and more graft resorption in defatted allografts. At 2 and 3 weeks, there was no significant difference in new bone area, but at 3 weeks the scintimetric activity was higher in defatted allografts, probably caused by an increased remodeling rate. Since defatting did not cause increased scintimetric activity in previous autograft studies, these findings could indicate that there is a detrimental immunologic influence on frozen allografts. There were no differences between defatted autografts and allografts. In a second experiment, the biomechanical properties of defatted bone were investigated with a compression test on defatted and frozen bone cylinders taken from the calf femoral neck. No difference in biomechanical properties was found. It was concluded that lipid extraction produced a graft that was better incorporated than a nondefatted graft, with no loss of mechanical function.
Tablets and Territories? Reconstructing Late Minoan IB Political Geography through Undeciphered Documents

In this article I explore the political geography of Neopalatial Crete through a multidisciplinary analysis of Late Minoan IB administrative documents. These documents testify to the existence of two levels in Minoan administration, a local level, represented by Linear A tablets, and a regional level, embodied by sealed documents. Both tablets and sealed documents reflect a considerable degree of regionalism, an observation that does not corroborate the thesis that LM IB Crete was a centralized state run by Knossos. On the basis of intersite differences in administration, it is argued here that administrative centers were managing and exploiting their own hinterland, without the interference of a central power. The so-called Knossos replica rings, which left identical impressions at various Neopalatial sites, cannot be directly connected with Knossos. Although perhaps testifying to the existence of an intensive supraregional network, the rings do not provide evidence that this was of a political nature. Some developments in material culture, script, and religion that occur at the beginning of the Neopalatial period are explained as politically motivated and caused by unifying aspirations of Knossos. By the time of the final destructions in LM IB, however, the regions of Crete had lapsed again into a looser network of regional centers, managing their own resources.
Non-Recourse Mortgage and Housing Price Bubble, Burst and Recovery

This paper investigates the impact of the non-recourse vs. recourse mortgage on the housing price dynamics in American major metropolitan areas (MSAs). We find strong evidence that non-recourse states experience faster price growth during the boom (2000 to 2006) as well as sharper price drop during the bust period (2006-2009). The result lends limited support to the notion that non-recourse states also experience faster price recovery in the rebound periods after the crisis (2009-2013). The volatility of housing price is larger in the non-recourse states than the recourse ones, in particular during the rebound period.