Miracle-dz/UniFAR-FTUs huggingface dataset:
Assembly and molecular order of two-dimensional peptoid nanosheets through the oil–water interface

Significance Peptoid nanosheets are an emerging class of 2D nanomaterials that have the potential for use in a variety of applications ranging from molecular sensors to artificial enzymes. Because peptoids are highly designable polymers, nanosheets provide a general platform on which to display an enormous diversity of functionalities. Nanosheets are known to form through a unique monolayer compression mechanism, catalyzed by the air–water interface. Here we demonstrate that nanosheets can be formed via adsorption of peptoids at an oil–water interface. Using vibrational sum frequency spectroscopy, we show that electrostatic interactions are essential in the formation of an ordered peptoid monolayer at the interface, a critical intermediate in the nanosheet assembly pathway. These findings open the door for enhancing the complexity and functionality of 2D nanomaterials. Peptoid nanosheets are a recently discovered class of 2D nanomaterial that form from the self-assembly of a sequence-specific peptoid polymer at an air–water interface. Nanosheet formation occurs first through the assembly of a peptoid monolayer and subsequent compression into a bilayer structure. These bilayer materials span hundreds of micrometers in lateral dimensions and have the potential to be used in a variety of applications, such as in molecular sensors, artificial membranes, and as catalysts. This paper reports that the oil–water interface provides another opportunity for growth of these unique and highly ordered peptoid sheets. The monolayers formed at this interface are found through surface spectroscopic measurements to be highly ordered and electrostatic interactions between the charged moieties, namely carboxylate and ammonium residues, of the peptoid are essential in the ability of these peptoids to form ordered nanosheets at the oil–water interface. Expanding the mechanism of peptoid nanosheet formation to the oil–water interface and understanding the crucial role of electrostatic interactions between peptoid residues in nanosheet formation is essential for increasing the complexity and functionality of these nanomaterials.
Extended conceptualisation of perceived usefulness: empirical test in the context of information system use continuance

A technology or an information system provides value to its users. Technology Acceptance Model (TAM) successfully operationalised such value as the degree that a system can improve users' job performance. The proposed construct, perceived usefulness, has been proved to be the most important factor for technology adoption. However, many scholars have called for further theoretical development to enrich this critical construct but not much effort has been put forward (Bagozzi, R.P., 2007. The legacy of the technology acceptance model and a proposal for a paradigm shift. Journal of the Association for Information Systems, 8 (4), 244–254; Benbasat, I. and Barki, H. , 2007. Quo vadis, TAM? Journal of the Association for Information Systems, 8 (4), 212–218). To respond to this call on deepening the conceptualisation of perceived usefulness, this article argues that system usefulness can be formulated beyond job performance improvement and explores several additional usefulness constructs based on well-established management concepts and human need theory. The information system (IS) use continuance model (Bhattacherjee, A., 2001. Understanding information systems continuance: An expectation-confirmation model. MIS Quarterly, 25 (3), 351–370) has been adopted as the theoretical foundation of this study because certain types of system usefulness can be recognised only in a use continuance stage. The empirical results of the research have validated most of the proposed constructs. Significant contributions to research and practice are identified and discussed.
Use of Marek’s disease vaccines: could they be driving the virus to increasing virulence?

Marek’s disease (MD) is an economically important neoplastic disease of poultry. MD almost devastated the poultry industry in the 1960s but the disease was brought under control after Marek’s disease herpesvirus (MDV) was identified and vaccines were developed. This is the first effective use of an antiviral vaccination to prevent a naturally occurring cancer in any species. MDV infection has many effects. Initially causing a cytolytic infection in B-lymphocytes, MDV infects activated T-lymphocytes where it becomes latent. In susceptible chicken genotypes MDV transforms CD4+ lymphocytes, causing visceral lymphomas and/or neural lesions and paralysis. Fully productive infection and shedding of infectious virus only occurs in the feather-follicle epithelium. Vaccination of newly-hatched chicks with live vaccines has been widely used to successfully control MD since the early 1970s. However, vaccinated chickens still become infected and shed MDV. Vaccine breaks have occurred with regularity and there is evidence that the use of MD vaccines could be driving MDV to greater virulence. MD continues to be a threat and a number of strategies have been adopted such as the use of more potent vaccines and vaccination of the embryonic stage to provide earlier protection. Recombinant MD vaccines are useful vectors and are being exploited to carry both viral and host genes to enhance protective immune responses. The future aim must be to develop a sustainable vaccine strategy that does not drive MDV to increased virulence.
An Acoustic Analysis of New Zealand English Vowels in Auckland

This study presents an acoustic analysis looking at phonetic diversity in Auckland. New Zealand English is often characterized by a lack of regional variation; however, this claim has been made without considering Auckland, New Zealand’s largest city. Over the last 30 years there has been increased migration to New Zealand, specifically to Auckland. In 35% of Auckland’s suburbs, no ethnic group represents more than 50% of the population. In addition, many speakers were born overseas, and many more have grown up using different varieties of English as the spoken norm. In this study, 40 New Zealand English speakers from three suburbs in Auckland (Mt. Roskill n= 14, Papatoetoe, n=13, Titirangi, n=13) were recorded. For our young group (n=33) the participants were aged between 18 and 25 years, and each suburb was evenly split between male and female participants. Speakers were either New Zealand born or arrived in the country under the age of seven. Our older group (n=7) were female speakers, all New Zealand born, and aged between 40 and 70 yrs. Vowels which had sentence stress were identified and extracted, and formant values were calculated at the vowel target. All formant tracks were hand checked. Over 8000 monophthong tokens and 4000 diphthongs were analysed in this study. Whilst no differences were found between young speakers from different suburbs, there were age effects. Further, speech from the young Auckland speakers was noticeably different to findings from other studies on New Zealand English. Most notably monophthongs TRAP and DRESS were lower than expected. In addition the first targets of the diphthongs FACE and GOAT have risen, and PRICE has fronted, for younger speakers from all suburbs. The thesis concludes discussing the implications of the results.
Growth factor deficiency in patients with ataxia telangiectasia

One prominent feature of patients with the autosomal recessive disease ataxia telangiectasia (AT) is somatic growth retardation. Due to their essential roles in development we examined levels of insulin‐like growth factor‐I (IGF‐I) as well as its main binding protein (IGFBP‐3) in a group of AT patients. Growth status of 19 patients was assessed by body mass index (BMI) and nutritional protocols. As suspected, BMI was low in AT patients despite adequate nutrition. Serum levels of IGF‐I were found to be below the 3rd percentile in 9 (56%) out of 16 patients and of IGFBP‐3 in 13 (81%) out of 16 patients. Our observations demonstrate that IGF‐I and IGFBP‐3 levels reflect the impaired growth status in patients with AT.
Deciphering the design of the tropomyosin molecule

The crystal structure at 2.0-Å resolution of an 81-residue N-terminal fragment of muscle α-tropomyosin reveals a parallel two-stranded α-helical coiled-coil structure with a remarkable core. The high alanine content of the molecule is clustered into short regions where the local 2-fold symmetry is broken by a small (≈1.2-Å) axial staggering of the helices. The joining of these regions with neighboring segments, where the helices are in axial register, gives rise to specific bends in the molecular axis. We observe such bends to be widely distributed in two-stranded α-helical coiled-coil proteins. This asymmetric design in a dimer of identical (or highly similar) sequences allows the tropomyosin molecule to adopt multiple bent conformations. The seven alanine clusters in the core of the complete molecule (which spans seven monomers of the actin helix) promote the semiflexible winding of the tropomyosin filament necessary for its regulatory role in muscle contraction.
Swiss Finance Institute Research Paper Series N ° 10 – 26 Price Impact and Portfolio Impact

We study survival, price impact and portfolio impact in heterogeneous economies. We show that, under the equilibrium risk-neutral measure, long-run price impact is in fact equivalent to survival, whereas longrun portfolio impact is equivalent to survival under an agent-specific, wealth-forward measure. These results allow us to show that price impact and portfolio impact are two independent concepts: a nonsurviving agent with no long-run price impact can have a significant long-run impact on other agents’ optimal portfolios.
Distributed series resistance effects in solar cells

A mathematical treatment is presented of the effects of one-dimensional distributed series resistance in solar cells. A general perturbation theory is developed, including consistently the induced spatial variation of diode current density and leading to a first-order equivalent lumped resistance of one third the total sheet resistance. For the case of diode characteristics of exponential type and distributed resistance of arbitrary size, unified numerical results are presented for both illuminated and dark characteristics. At high forward dark currents, the distributed series resistance is shown to cause an effective doubling of the "diode quality factor. "
When do predatory mites (Phytoseiidae) attack? Understanding their diel and seasonal predation patterns

Predatory mites of the Phytoseiidae family are considered one of the most important groups of natural enemies used in biological control. The behavioral patterns of arthropods can differ greatly daily and seasonally; however, there is a lack of literature related to Phytoseiidae diel and seasonal predation patterns. The predatory activity of three phytoseiid species (two Tetranychidae‐specialists, Phytoseiulus persimilis and Neoseiulus californicus, and one omnivore, Euseius stipulatus) that occur naturally in Spanish citrus orchards was observed under laboratory conditions in winter and summer. The temperature and photoperiod of the climatic chamber where the mites were reared did not change during the experiment. Our study demonstrates that phytoseiids can exhibit diel and seasonal predatory patterns when feeding on Tetranychus urticae (Acari: Tetranychidae). Neoseiulus californicus was revealed to be a nocturnal predator in summer but diurnal in winter. In contrast, P. persimilis activity was maximal during the daytime, and E. stipulatus showed no clear daily predation patterns. The predatory patterns described in this study should be taken into account when designing laboratory studies and also in field samplings, especially when applying molecular techniques to unveil trophic relationships.
Adsorption of lead (II) ions onto diatomite from aqueous solution: Mechanism, isotherm and kinetic studies

This paper assesses the performance in seawater environment of concrete mixes based on alkali-activated (AA) cements; these are proposed as an alternative to Ordinary Portland Cement (OPC) to address environmental footprint of cement production and to find new uses for waste materials. The proposed AA cements contained an industrial by-product, ground granulated blast furnace slag (GGBS) and a waste material, paper sludge ash (PSA). Mixes were made with fresh water and seawater respectively and were cured either in freshwater or seawater to simulate future exposure environments. The compressive strength at different curing times and a number of durability-related properties of AA mixes in marine environment were investigated and compared to those of OPC systems. The findings indicated that mixing with seawater rather than freshwater enhanced the performance of the AA mixes in terms of compressive strength and durability (resistance to chloride and sulphate attack). In a seawater environment the AA slag concrete mixes with PSA had the lowest porosity, which can be linked to their good durability performance. The study gives promise for the suitability of the tested alkaliactivated concrete mixes in seawater environments.
Assessing the diversity of the g23 gene of T4‐like bacteriophages from Lake Baikal with high‐throughput sequencing

Abstract Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4‐like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obtained by the Sanger method before. We obtained 33 701 sequences of the g23 gene fragments, 141 operational taxonomic units (OTUs) of which were identified. 86 OTUs (60.9%) had the closest relatives from lakes Bourget and Annecy, and 28 OTUs (19.8%) had the highest identity with the Baikal g23 clones, which had been previously identified in the northern and southern basins of the lake by the Sanger method. The remaining OTUs were similar to the clones from other ecosystems. We showed a high genetic diversity of T4‐type bacteriophages and a genetic difference with the phage communities from other ecosystems. Figure. No Caption available.
A multicenter study to clarify the optimal HCV-RNA negative period during combined therapy with pegylated interferon plus ribavirin in patients with chronic hepatitis caused by HCV genotype 2.

OBJECTIVE A multicenter open trial was performed to clarify the optimal duration of combined pegylated interferon (Peg-IFN) plus ribavirin therapy in patients with chronic hepatitis caused by HCV genotype 2. METHODS A total of 100 patients seen between 2005 and 2007 received the combination therapy for 4 to 52 weeks. The cutoff value of the HCV-RNA-negative (titers under 1.7 Log IU/mL) period during the therapy to predict sustained virological response (SVR) was determined by ROC curve and multivariate logistic regression analyses. The result was validated in 48 patients between 2008 and 2009. RESULTS SVR was achieved in 78 patients. Serum HCV-RNA titers decreased to less than 1.7 Log IU/mL at 4 weeks of the therapy in 60 patients. The SVR rate in these patients was 85%, which was significantly higher than that of remaining 40 patients with a SVR rate of 68%. An HCV-RNA-negative period of ≥17 weeks was selected as the cutoff value, which showed a significant odds ratio of 4.77 for SVR. Among the 35 patients who showed a decrease of the serum HCV-RNA of less than 1.7 Log IU/mL between 8 and 16 weeks of therapy, the SVR rate was significantly higher in 16 patients with a serum HCV-RNA-negative period of ≥17 weeks (94%) than in 19 patients in whom the period was less than 17 weeks (63%). Similar results were obtained in the subsequent validation study. CONCLUSION Prolonged combined Peg-IFN plus ribavirin therapy, with an HCV-RNA-negative period of ≥17 weeks, yielded good therapeutic outcomes in patients with chronic HCV genotype 2 hepatitis.
The TF-603A/G gene promoter polymorphism and circulating monocyte tissue factor gene expression in healthy volunteers

Summary Three single nucleotide polymorphisms (-603A/G, -1322C/T, -1812C/T) and one deletion/insertion polymorphism (-1208D/I) are present in the tissue factor (TF) gene promoter sequence. These polymorphisms are in complete linkage disequilibrium, determining two haplotypes with almost equal frequency. The -603A/-1208D/-1322C/-1812C haplotype, presently defined as TF-603A, has been linked to venous thromboembolic disease, with a potentially protecting effect. The effects of the TF-603A/G gene polymorphism on monocyte gene expression and on a whole-blood clotting time (WBCT) are not known. We determined the WBCT in basal conditions (H0) and after 4 hours of LPS stimulation ex vivo (H4LPS) on blood samples from 100 young healthy caucasian male subjects on 2 visits, 7 days apart. Monocyte TF mRNA was quantified at H0 and H4LPS by real-time quantitative reverse-transcription PCR. The monocyte TF mRNA values determined at the first and second visits were concordant. In H4LPS samples, TF mRNA levels were increased 70-fold. The TF-603A haplotype was associated with 40%-lower TF mRNA levels at H0 (P=0.0002) and this association followed the same trend but was no longer significant at H4LPS. At H4LPS, TF mRNA levels were associated with WBCT shortening (P=0.0003). WBCT at H0 was not concordant over time, precluding any genotype-phenotype analysis. WBCT at H4LPS was concordant over time but was not related to the TF-603A/G polymorphism. The TF-603A/G gene promoter polymorphism thus significantly influences constitutive TF gene expression in human monocytes but has no major effect on TF gene expression or on WBCT in LPS stimulated conditions.
Identification of ligand recognition sites in heat-stable enterotoxin receptor, membrane-associated guanylyl cyclase C by site-directed mutational analysis

Guanylyl cyclase C (STaR), a receptor protein for heat-stable enterotoxin (STa) elaborated by Escherichia coli, is associated with and spans the plasma membrane of mammalian intestinal cells. The extracellular domain functions in the binding of STa and the association of each domain to an oligomeric form. Two amino acid residues, Arg-136 and Asp-347, were identified as the residues binding to STa in the extracellular domain of pig STaR by site-directed mutagenesis and analysis of expression on 293T cells. Replacement of these residues by other amino acid residues resulted in the loss of binding of pig STaR to STa, and as a result, STa-induced guanylyl cyclase activity was eliminated. Furthermore, mutation in a region (from Asp-347 to Val-401) which is close to the transmembrane domain caused a significant reduction in both STa-binding activity and guanylyl cyclase catalytic activity. These results suggest that the region adjacent to the transmembrane domain plays an important role in facilitating a favorable conformation of STaR for STa binding.
Associations among progeny tests of single or pooled lactations.

Genetic correlations between Predicted Difference for first and second, first and third, and second and third lactations milk were . 82, . 62, and . 94 and for Predicted Difference percent fat . 94 for the three associations. Regression coefficients for following on previous lactation Predicted Difference were less than unity; thus, Predicted Difference from first lactation might be overweighted when age adjusted and pooled with second and third lactations. Regressions of Predicted Difference milk of sons on sires for first, second, third, and pooled lactation records were . 32 +/- 18, . 42 +/- . 21, . 56 +/- . 31, . 35 +/- . 15, and intraclass correlations between half brothers were . 09 +/- . 08, . 28 +/- . 11, . 25 +/- . 11, and . 12 +/- 10. Predicted Differences for second and third lactations were, thus, near the theoretical expectation of . 5 and . 25 for regressions of son on sire and between half-brother correlations but lower for Predicted Differences of first and pooled lactations. Variance of Predicted Difference of first lactation between sires of sons was small compared with the variance of sons within sires. It was postulated that effective selection on the sire-to-sire path on Predicted Difference of first lactation had reduced predictability on the sire-to-sire improvement path. An interaction of sire by number of lactations may be inferred from the incomplete genetic correlations between Predicted Differences of first and following lactations and from the reduction in the variance between sire in first but not in later lactations. Progeny tests of single lactation seem warranted for the production profile of dairy sires.
Efficacy of intradermal mesotherapy in cellulite reduction – Conventional and high-frequency ultrasound monitoring results

ABSTRACT Background: Cellulite affects the majority of women and is an unacceptable cosmetic defect. Therefore, effective methods for cellulite reduction are being sought. Intradermal mesotherapy is one of such methods. Objective: The aim of the study was to assess the efficacy of intradermal mesotherapy in cellulite reduction, using conventional and high-frequency ultrasound. Methods: Twenty-one women with cellulite underwent a series of intradermal mesotherapy procedures. The following parameters were assessed: thickness of epidermis, dermis and hypodermis, echogenicity of dermis and the surface area of serrated hypodermis–dermis junction. Furthermore, the thigh circumference was measured; body mass index and cellulite severity were assessed based on photographs using Nürnberger–Müller’s scale. Results: Intradermal mesotherapy reduced severity of cellulite. The surface area of serrated hypodermis–dermis junction and hypodermis thickness decreased significantly as compared to baseline. Cellulite reduction was also confirmed by palpation, decreased thigh circumference and the Nürnberger–Müller’s grade. There were no statistically significant changes in epidermis or dermis thickness, body weight and the BMI. Conclusion: Intradermal mesotherapy offers effective cellulite reduction. It is a simple and safe treatment, which makes it popular. Further research in mesotherapy is essential due to a limited number of published studies. Ultrasound is a useful method to monitor intradermal mesotherapy and assess its efficacy.