Miracle-dz/UniFAR-FTUs huggingface dataset:
Stress, Breakfast Cereal Consumption and Objective Signs of Upper Respiratory Tract Illnesses

Recent research has shown that both negative life events and breakfast cereal consumption are associated with the incidence and severity of subjective symptoms of upper respiratory tract illnesses (URTIs) Two studies were conducted to examine whether objective markers of illness were also associated with stress and breakfast cereal consumption. The results from the first study showed that regular breakfast cereal consumption was associated with lower sub-lingual temperatures in volunteers with URTIs. Stress had no effect on temperature. In the second study nasal secretion weight was lower in regular breakfast cereal consumers but was not influenced by stress. These studies suggest that breakfast cereal consumption is associated with reduced illness seventy and that this does not reflect stress levels. Further research is now required to determine whether such results are directly due to cereal consumption or reflect correlated attributes, such as other aspects of the diet.
Signatures of the superfluid to Mott insulator transition in equilibrium and in dynamical ramps

We investigate the equilibrium and dynamical properties of the Bose-Hubbard model and the related particle-hole symmetric spin-1 model in the vicinity of the superfluid to Mott insulator quantum phase transition. We employ the following methods: exact-diagonalization, mean-field (Gutzwiller), cluster mean-field, and mean-field plus Gaussian fluctuations. In the first part of the paper we benchmark the four methods by analyzing the equilibrium problem and give numerical estimates for observables such as the density of double occupancies and their correlation function. In the second part, we study parametric ramps from the superfluid to the Mott insulator and map out the crossover from the regime of fast ramps, which is dominated by local physics, to the regime of slow ramps with a characteristic universal power law scaling, which is dominated by long wavelength excitations. We calculate values of several relevant physical observables, characteristic time scales, and an optimal protocol needed for observing universal scaling.
Cellular MicroRNAs 200b and 429 Regulate the Epstein-Barr Virus Switch between Latency and Lytic Replication

ABSTRACT We previously showed that the cellular proteins ZEB1 and ZEB2/SIP1 both play key roles in regulating the latent-lytic switch of Epstein-Barr Virus (EBV) by repressing BZLF1 gene expression. We investigated here the effects of cellular microRNA (miRNA) 200 (miR200) family members on the EBV infection status of cells. We show that miR200b and miR429, but not miR200a, can induce EBV-positive cells into lytic replication by downregulating expression of ZEB1 and ZEB2, leading to production of infectious virus. The levels of miR200 family members in EBV-infected cells strongly negatively correlated with the levels of the ZEBs (e.g., −0.89 [P < 0.001] for miR429 versus ZEB1) and positively correlated with the degree of EBV lytic gene expression (e.g., 0.73 [P < 0.01] for miR429 versus BZLF1). The addition of either miR200b or miR429 to EBV-positive cells led to EBV lytic reactivation in a ZEB-dependent manner; inhibition of these miRNAs led to decreased EBV lytic gene expression. The degree of latent infection by an EBV mutant defective in the primary ZEB-binding site of the EBV BZLF1 promoter was not affected by the addition of these miRNAs. Furthermore, EBV infection of primary blood B cells led to downregulation of these miRNAs and upregulation of ZEB levels. Thus, we conclude that miRNAs 200b and 429 are key regulators via their effects on expression of ZEB1 and ZEB2 of the switch between latent and lytic infection by EBV and, therefore, potential targets for development of new lytic induction therapeutics with which to treat patients with EBV-associated malignancies.
Proteomics of tumor‐specific proteins in cerebrospinal fluid of patients with astrocytoma: Usefulness of gelsolin protein

Changes in cerebrospinal fluid (CSF) composition have been shown to accurately reflect pathological processes in the CNS, and are potential indicators of abnormal CNS states, such as tumor growth. To detect biomarkers in high‐grade astrocytomas, the differential expression of proteins in the cerebrospinal fluid was analyzed from two cases each of diffuse astrocytoma (grade II), and glioblastoma (grade IV) using agarose 2‐D gel electrophoresis (2‐DE). It was found that the expression of gelsolin protein decreased with histological grade. To examine whether gelsolin is a useful indicator of tumor aggressiveness or patient outcome, its expression was further studied on immunohistochemistry in 41 formalin‐fixed and paraffin‐embedded astrocytomas. The positive cell rate of gelsolin in tumors was 59.4% in grade II, 30.0% in grade III and 29.4% in grade IV, respectively. Gelsolin expression was significantly lower in high‐grade astrocytomas (grade III or IV) than in low‐grade astrocytomas (grade II; P < 0.05). Moreover, in astrocytomas the overall survival of patients in the low‐expression group was significantly poorer than in the high expression group (P < 0.05). These data suggest that gelsolin is a prognostic factor in astrocytoma.
Synthetic observations of molecular clouds in a galactic centre environment – I. Studying maps of column density and integrated intensity

We run numerical simulations of molecular clouds, adopting properties similar to those found in the central molecular zone (CMZ) of the Milky Way. For this, we employ the moving mesh code AREPO and perform simulations which account for a simplified treatment of timedependent chemistry and the non-isothermal nature of gas and dust. We perform simulations using an initial density of n(0) = 10(3) cm(-3) and a mass of 1.3 x 10(5) M-circle dot. Furthermore, we vary the virial parameter, defined as the ratio of kinetic and potential energy, alpha = E-kin/|E-pot|, by adjusting the velocity dispersion. We set it to alpha = 0.5, 2.0 and 8.0, in order to analyse the impact of the kinetic energy on our results. We account for the extreme conditions in the CMZ and increase both the interstellar radiation field (ISRF) and the cosmic ray flux (CRF) by a factor of 1000 compared to the values found in the solar neighbourhood. We use the radiative transfer code RADMC-3D to compute synthetic images in various diagnostic lines. These are [C (Pi]) at 158 mu m, [O (Iota)] (145 mu m), [O (Iota)] (63 mu m), (CO)-C-12 (J = 1 -> 0) and (CO)-C-13 (J = 1 -> 0) at 2600 and 2720 mu m, respectively. When alpha is large, the turbulence disperses much of the gas in the cloud, reducing its mean density and allowing the ISRF to penetrate more deeply into the cloud's interior. This significantly alters the chemical composition of the cloud, leading to the dissociation of a significant amount of the molecular gas. On the other hand, when alpha is small, the cloud remains compact, allowing more of the molecular gas to survive. We show that in each case the atomic tracers accurately reflect most of the physical properties of both the H-2 and the total gas of the cloud and that they provide a useful alternative to molecular lines when studying the interstellar medium in the CMZ.
Continuous cultivation of human hamstring tenocytes on microcarriers in a spinner flask bioreactor system

Tendon healing is a time consuming process leading to the formation of a functionally altered reparative tissue. Tissue engineering‐based tendon reconstruction is attracting more and more interest. The aim of this study was to establish tenocyte expansion on microcarriers in continuous bioreactor cultures and to study tenocyte behavior during this new approach. Human hamstring tendon‐derived tenocytes were expanded in monolayer culture before being seeded at two different seeding densities (2.00 and 4.00 × 106 cells/1000 cm2 surface) on Cytodex™ type 3 microcarriers. Tenocytes' vitality, growth kinetics and glucose/lactic acid metabolism were determined dependent on the seeding densities and stirring velocities (20 or 40 rpm) in a spinner flask bioreactor over a period of 2 weeks. Gene expression profiles of tendon extracellular matrix (ECM) markers (type I/III collagen, decorin, cartilage oligomeric protein [COMP], aggrecan) and the tendon marker scleraxis were analyzed using real time detection polymerase chain reaction (RTD‐PCR). Type I collagen and decorin deposition was demonstrated applying immunolabeling. Tenocytes adhered on the carriers, remained vital, proliferated and revealed an increasing glucose consumption and lactic acid formation under all culture conditions. “Bead‐to‐bead” transfer of cells from one microcarrier to another, a prerequisite for continuous tenocyte expansion, was demonstrated by scanning electron microscopy. Type I and type III collagen gene expression was mainly unaffected, whereas aggrecan and partly also decorin and COMP expression was significantly downregulated compared to monolayer cultures. Scleraxis gene expression revealed no significant regulation on the carriers. In conclusion, tenocytes could be successfully expanded on microcarriers. Therefore, bioreactors are promising tools for continuous tenocyte expansion. © 2013 American Institute of Chemical Engineers Biotechnol. Prog. , 30:142–151, 2014
Interface conditions on postverbal subjects: A corpus study of L2 English

This paper investigates how syntactic knowledge interfaces with other cognitive systems by analysing the production of postverbal subjects, V(erb)–S(ubject) order, in an L1 Spanish–L2 English corpus and a comparable English native corpus. VS order in both native and L2 English is shown to be constrained by properties operating at three interfaces: (i) lexicon–syntax: the verb is unaccusative (Unaccusative Hypothesis); (ii) syntax–discourse: the subject is focus (End-Focus Principle) and (iii) syntax–phonology: the subject is heavy (End-Weight Principle). We show that, since learners produce VS under the same interface conditions as native speakers, unaccusativity is a necessary but not a sufficient condition for VS production. However, learners overproduce VS and make persistent errors in their syntactic encoding. Our findings support recent proposals that these difficulties stem from problems at coordinating syntactic knowledge with knowledge from other external systems, but they suggest that the nature of such difficulties is not external to the syntax.
Noninvasive detection of activating estrogen receptor 1 (ESR1) mutations in estrogen receptor-positive metastatic breast cancer.

BACKGROUND Activating mutations in the estrogen receptor 1 (ESR1) gene are acquired on treatment and can drive resistance to endocrine therapy. Because of the spatial and temporal limitations of needle core biopsies, our goal was to develop a highly sensitive, less invasive method of detecting activating ESR1 mutations via circulating cell-free DNA (cfDNA) and tumor cells as a "liquid biopsy. " METHODS We developed a targeted 23-amplicon next-generation sequencing (NGS) panel for detection of hot-spot mutations in ESR1, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA), tumor protein p53 (TP53), fibroblast growth factor receptor 1 (FGFR1), and fibroblast growth factor receptor 2 (FGFR2) in 48 patients with estrogen receptor-α-positive metastatic breast cancer who were receiving systemic therapy. Selected mutations were validated using droplet digital PCR (ddPCR). RESULTS Nine baseline cfDNA samples had an ESR1 mutation. NGS detected 3 activating mutations in ESR1, and 3 hot-spot mutations in PIK3CA, and 3 in TP53 in baseline cfDNA, and the ESR1 p.D538G mutation in 1 matched circulating tumor cell sample. ddPCR analysis was more sensitive than NGS and identified 6 additional baseline cfDNA samples with the ESR1 p.D538G mutation at a frequency of <1%. In serial blood samples from 11 patients, 4 showed changes in cfDNA, 2 with emergence of a mutation in ESR1. We also detected a low frequency ESR1 mutation (1.3%) in cfDNA of 1 primary patient who was thought to have metastatic disease but was clear by scans. CONCLUSIONS Early identification of ESR1 mutations by liquid biopsy might allow for cessation of ineffective endocrine therapies and switching to other treatments, without the need for tissue biopsy and before the emergence of metastatic disease.
Variable Recruitment Testing of Pneumatic Artificial Muscles for Robotic Manipulators

This paper investigates the orderly recruitment of pneumatic artificial muscles for efficient torque production in a robotic manipulator. Pneumatic artificial muscles (PAMs) are arranged in a parallel bundle, and independently-controlled “motor units” are employed to imitate the structure and function of human skeletal muscle. Simulated cycling tests are conducted on a model of the robotic manipulator to quantify the benefits of variable recruitment, and experimental testing is performed to validate the simulated predictions. Results reveal a distinct relationship between recruitment and system efficiency. Key factors influencing the value of a variable recruitment strategy include nonlinear PAM bladder elasticity, pneumatic losses, and dissipative forces in the robotic joint. Recruitment guidelines are proposed to maximize efficiency over a range of payload masses. The potential challenges associated with maintaining smooth motion control during discrete transitions in recruitment are also identified and discussed.
New insights into perforator vein incompetence.

BACKGROUND there has been much controversy on the role of perforator veins in the development of chronic venous disease (CVD). This study was designed to determine the duration and direction of flow of lower limb perforator veins (PVs) in relation to their location, diameter and competency status of superficial and deep veins, in healthy volunteers and patients with different grades of CVD. PATIENTS AND METHODS thirty limbs in 15 symptom-free volunteers and 103 limbs in 75 patients with signs and symptoms of CVD were examined with colour-flow duplex scanning. Superficial, perforator and deep veins were studied in the standing and sitting positions. Flow-velocity characteristics, the number and maximum PV diameter at the deep fascia and subfascially were determined. A PV was considered incompetent when the outward flow lasted >0.5 s. RESULTS 581 PVs were found in the patients and 106 in the volunteers. 163 PVs (28%) were incompetent in the first group and none in the latter. The total number of PVs and the number of incompetent PVs per limb increased significantly with the severity of CVD. The mid-calf area had more competent and incompetent PVs in patients (p <0.01). Mean diameter of incompetent PVs in all the CVD classes was significantly larger than that of competent PVs. Competent PVs tended to be larger with increasing severity of CVD and they were significantly larger in the CVD classes 4 to 6 compared to controls (p <0.01). Subfascial PV diameter was markedly larger than that at the fascial level (p <0.001) regardless of the CVD class. A subfascial PV diameter of >3.9 mm (95% CI 3.4 to 4.4 mm) indicated incompetence. However, the reverse was not true, because about a third of incompetent PVs had a subfascial diameter of <3.9 mm. Both competent and incompetent PVs were smaller when located at the lower thigh, knee, ankle and anterior aspect of the calf than those found in the rest of the calf and mid-thigh (p =0. 03). Both inward and outward flow was found more often in patients than in controls (70/418 vs. 9/106, p =0.048). Most incompetent PVs had outward flow alone (126, 77%). PV incompetence was most frequently associated with reflux in superficial veins (120, 74% (p <0.0001), followed by reflux in both the superficial and deep veins (34, 21%) and reflux in the deep veins alone (9, 5%). The mean duration of outward flow was markedly longer in the presence of both superficial and deep vein reflux compared to superficial (p <0.001) or deep vein reflux alone (p <0.0001). CONCLUSIONS the number of incompetent PVs and the diameter of both competent and incompetent PV increases with the severity of CVD. Bidirectional PV flow is more common in patients than in normal volunteers, while 77% of the incompetent PVs have outward flow alone. PV incompetence is most often associated with reflux in the superficial veins, indicating that deep venous reflux is rarely the primary cause of PV insufficiency.
Tolerability of once-weekly alendronate in patients with osteoporosis: a randomized, double-blind, placebo-controlled study.

OBJECTIVE To compare the upper gastrointestinal (GI) tract tolerability of once-weekly oral alendronate, 70 mg, and placebo. PATIENTS AND METHODS This was a 12-week multicenter, randomized, double-blind, placebo-controlled study. The first patient initiated treatment on June 5, 2000, and the last patient completed treatment on March 1, 2001. The study enrolled 450 postmenopausal women and men with osteoporosis (224 took alendronate, 226 took placebo) who were ambulatory and community dwelling at 48 outpatient study centers in the United States. By design, approximately half of the patients were naive to bisphosphonates. The primary end point was upper GI tract tolerability based on the incidence of any upper GI tract adverse events. Secondary end points included the number of discontinuations due to drug-related upper GI tract adverse events and the change from baseline in bone resorption, assessed by the urinary N-telopeptide-creatinine ratio at 12 weeks. A subgroup analysis of the primary and secondary end points was performed on the patients stratified by prior bisphosphonate use. The safety and tolerability of the weekly alendronate and placebo regimens were captured as clinical and laboratory adverse events. RESULTS A total of 11% of the alendronate patients and 13% of the placebo patients reported an upper GI tract adverse event. Discontinuations due to drug-related upper GI tract adverse events occurred in 3% of alendronate patients and 1% of placebo patients. The differences between the treatment groups for the primary and secondary end points were not significant. For the primary end point, the upper limit of the 95% confidence interval of the difference was well within the prespecified 14% comparability bound (-2.2%; 95% confidence interval, -8.3% to 3.9%). The overall incidence of upper GI tract adverse events was lower in the subgroup of patients with prior bisphosphonate exposure (8%) than in those who were bisphosphonate naive (16%). However, regardless of prior bisphosphonate exposure, the incidence of upper GI tract adverse events was similar between the alendronate and placebo patients. The urinary N-telopeptide-creatinine ratio showed a significant decrease in the alendronate patients (72% of baseline, P<.001) compared with a slight increase in the placebo patients (106% of baseline) at week 12. CONCLUSION In this 3-month study, the incidence of upper GI tract adverse events in patients treated with once-weekly alendronate, 70 mg, was comparable to that with placebo.
A MORPHOMETRIC STUDY OF SUPRATROCHLEAR FORAMEN OF THE HUMERUS IN WESTERN INDIAN DRY BONE SAMPLE

Address for Correspondence: Dr. Mohini M Joshi, Associate Professor, Department of Anatomy, Rural Medical College, Pravara Institute of Medical Sciences, Loni, Dist. Ahmadnagar Maharashtra, India. Mobile No: +919762601050 E-Mail: atharvamohini@gmail.com Background: In the distal end of humerus, the coronoid fossa in front and the olecranon fossa behind is separated by a thin lamina of bone, the coronoidolecranon septum (COS). This thin plate of bone sometimes become transparent or perforated in some cases and give rise to a foramen called septal aperture or commonly known as supratrochlear foramen (STF). Objective: The present study describes the incidence, different shapes, morphological features, and clinical importance of foramen in the humerus of Western Indian population. Materials and Methods: The presence of supratrochlear foramen was studied in 85 dry adult humeri of unknown sex and of both sides. A total of 170 (85 right sided and 85 left sided) Adult human dried humeri were obtained. Mean and standard deviations for different measurement of foramina was calculated. Unpaired t test was applied to test the significance of difference of different measurements of the foramen of the humerus between left and right side. Results: Out of total 170 humeri studied STF were present in 61(35.88%) humeri. Most of foramen were oval shaped that is 32(37.64) on left side and 08(9.41%) on right side. Most of septum on left side 27 (31.76%) and right side of humeri 35 (41.17%) were having translucent septum. Difference between measurements of vertical and transverse diameter of right and left sided foramina were not statistically significant (p>0.05). Difference between measurements of distance of foramen from medial epicondyle and lateral epicondyle and midpoint of trochlea on right and left sides were not statistically significant (p>0.05). Conclusions: The supratrochlear foramen has much clinical importance. The foramen should be kept in mind during any surgical or radiological intervention to aid in proper diagnosis and treatment.
A Comparison of the Effect of pH on the Biodegradation of Two Synthetic Absorbable Sutures

The differences in hydrolytic degradation of two size 2–0 synthetic absorbable sutures, Polyglycolic acid (Dexon®) and Poiy(glycolide-lactide) (Vicryl®), in the buffer media of three different pH levels ranging from 5.25 to 10.09, were compared in terms of the percentage retention of tensile strength. It was found that Vicryl sutures, in general, exhibited better retention of tensile strength than Dexon sutures within the studied pH range and extent of hydrolysis. When comparing Vicryl with Dexon sutures, the former was only slightly better than Dexon sutures, in the case of an acid environment. The former, however, retained better strength than the latter in a physiological pH (7.44) and under high-alkaline conditions (10.09). Among the three pH levels tested, Vicryl sutures retained the highest tensile strength in a physiological pH and showed a faster loss of tensile strength in both acidic and high-alkaline conditions. Dexon sutures, however, exhibited better retention of tensile strength in the acidic medium than in an alkaline medium. This pH dependent hydrolysis of Dexon and Vicryl sutures should command surgeons' attention in their selection of synthetic absorbable suture materials for particular needs.
Students' epistemologies about experimental physics: Validating the Colorado Learning Attitudes about Science Survey for Experimental Physics

Student learning in instructional physics labs represents a growing area of research that includes investigations of students' beliefs and expectations about the nature of experimental physics. To directly probe students' epistemologies about experimental physics and support broader lab transformation efforts at the University of Colorado Boulder (CU) and elsewhere, we developed the Colorado Learning Attitudes about Science Survey for Experimental Physics (E-CLASS). Previous work with this assessment has included establishing the accuracy and clarity of the instrument through student interviews and preliminary testing. Several years of data collection at multiple institutions has resulted in a growing national data set of student responses. Here, we report on results of the analysis of these data to investigate the statistical validity and reliability of the E-CLASS as a measure of students' epistemologies for a broad student population. We find that the E-CLASS demonstrates an acceptable level of both validity and reliability on measures of, item and test discrimination, test-retest reliability, partial-sample reliability, internal consistency, concurrent validity, and convergent validity. We also examine students' responses using Principal Component Analysis and find that, as expected, the E-CLASS does not exhibit strong factors.