Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic relationships among Leptographium terebrantis and the mycangial fungi of three western Dendroctonus bark beetles

Morphology, mitochondrial DNA (mtDNA) restriction fragment polymorphisms (RFLPs) and nuclear DNA (nDNA) fingerprinting were used to clarify relationships among the morphologically similar Ophiostoma and Leptographium species associated with mycangia of three Dendroctonus bark beetles (Ophiostoma clavigerum associated with both D. ponderosae and D. jeffreyi, and L. pyrinum associated with D. adjunctus), as well as a closely related nonmycangial bark beetle associate (L. terebrantis). Most isolates of O. clavigerum form long (40–70 μm), septate conidia, while all isolates of L. terebrantis and L. pyrinum form conidia less than 17.0 μm in length. The conidia of L. pyrinum are pyriform, with truncate bases, while the conidia of the other species form only slightly truncate bases. Conidial masses of L. terebrantis are creamy yellow, while the conidial masses of the other species are white. Nuclear DNA fingerprints resulting from probing PstI restrictions with the oligonucleotide probe (CAC)5 and HaeIII and MspI restrictions of mtDNA, exhibited three major clusters. In the dendrogram developed from mtDNA RFLPs, the L. pyrinum isolates formed one cluster, while the majority of O. clavigerum isolates, including all D. jeffreyi isolates, formed another. A third cluster was composed of all L. terebrantis isolates, as well as several O. clavigerum isolates from D. ponderosae. The inclusion of some O. clavigerum isolates in the L. terebrantis cluster suggests that horizontal transfer of mtDNA has occurred among these fungi. The nDNA dendrogram also exhibited three clusters, and most isolates of L. pyrinum, L. terebrantis and O. clavigerum grouped separately; however, one isolate of O. clavigerum grouped with the L. terebrantis isolates, while one isolate of L. terebrantis grouped with O. clavigerum. No genetic markers were found that distinguished between O. clavigerum associated with D. ponderosae and O. clavigerum associated with D. jeffreyi. Ophiostoma clavigerum might be a recently diverged morphological variant of L. terebrantis, with special adaptations for grazing by young adults of D. jeffreyi and D. ponderosae. The anamorph of O. clavigerum, Graphiocladiella clavigerum, is transferred to Leptographium.
Influence of Ionic Strength on Hoechst 33258 Binding with DNA

Abstract The binding of Hoechst 33258 with DNA at various ionic strengths of solution and different ligand concentrations has been investigated. Existence of more than one type of interactions of Hoechst 33258 with DNA has been revealed, which were very sensitive to the ionic strength. Hoechst 33258 doesn't show specificity to AT sequences of DNA at low ionic strength. High affinity binding mode becomes obvious at high ionic strength. The values of binding constants and binding site sizes for revealed strong and weak interactions have been determined.
Effects of Chronic Glucocorticoid Administration on Insulin-Degrading Enzyme and Amyloid-Beta Peptide in the Aged Macaque

Insulin-degrading enzyme (IDE) has been identified as a candidate protease in the clearance of amyloid-β (Aβ) peptides from the brain. IDE activity and binding to insulin are known to be inhibited by glucocorticoids in vitro. In Alzheimer disease (AD), both a decrease in IDE levels and an increase in peripheral glucocorticoid levels have been documented. Our study investigated the effects of glucocorticoid treatment on IDE expression in vivo in 12 nonhuman primates (Macaca nemestrina). Year-long, high-dose exposure to the glucocorticoid cortisol (hydrocortisone acetate) was associated with reduced IDE protein levels in the inferior frontal cortex and reduced IDE mRNA levels in the dentate gyrus of the hippocampus. We assessed Aβ40 and Aβ42 levels by ELISA in the brain and in plasma, total plaque burden by immunohistochemistry, and relative Aβ1-40 and Aβ1-42 levels in the brain by mass spectrometry. Glucocorticoid treatment increased Aβ42 relative to Aβ40 levels without a change in overall plaque burden within the brain, while Aβ42 levels were decreased in plasma. These findings support the notion that glucocorticoids regulate IDE and provide a mechanism whereby increased glucocorticoid levels may contribute to AD pathology.
Craving decrease with topiramate in outpatient treatment for cocaine dependence: an open label trial.

OBJECTIVE To evaluate anticraving action and tolerability of topiramate in cocaine user treatment. METHOD Male users of inhaled cocaine which met criteria for cocaine dependence (Diagnostic and Statistical Manual of Mental Disorders, fourth edition) were selected for outpatient 12-week, open label trial with topiramate; individual dosage ranged between 25-300 mg/day. Main clinical variables were abstinence rate, craving intensity, frequency and duration, adherence, dropouts, side effects and impulsivity measure through Barratt Impulsivity Scale. Patients received assertive strategic counseling for abstinence assistance and medication monitoring evaluation every two weeks. Comparative analysis was made with intention to treat, missing values were replaced (last observation carried forward), and significance level was 5%. RESULTS Adherence to treatment was 57% (at least three evaluations), 32% dropped out (one evaluation). There were no severe side effects. Negative test average was 25.4% (31.2). Significant reduction in craving intensity and duration was observed in 25% of the sample. No statistical significant reduction in craving frequency was observed in 7.1%. Increase in frequency was observed in 10.7% and 82.1% did not present any variation. No significant statistical variations in Barratt Impulsivity Scale or in the total score were found in the final evaluation when compared to baseline. CONCLUSION More randomized placebo-controlled trials with topiramate for cocaine dependants should be performed to evaluate preliminary evidence.
Economic Analysis of Paddy Rice Production in Niger Delta Region of Nigeria

The study examined economic analysis of paddy rice production in Niger Delta Region of Nigeria in view to determine how profitable the crop is in the study area and how inputs or resources used in paddy rice production significantly affect the crop’s production. The data for the study was collected from 300 rice farmers in three out of nine states in the region based on their intensity in rice production in the study area using multistage and simple random sampling technique. Data analysis was carried out using descriptive statistics, multiple regression model and profitability model. Rice production was found to be profitable as farmers realized ₦300,071.84 as Net farm Income. It was also found that resources used in paddy rice production significantly affect paddy rice production. The result indicated that mean age of paddy rice farmers was 49 years, the mean farming experience was 17 years while mean cultivated land was 2.32 hectares It was recommended that Reviewing the Land Use Act of 1990 is critical so that most of the fertile land held by government will be released to rice farmers, also providing skilled labour required in rice production and easy evacuation of farm produce will go a long way in increasing the production of paddy rice in the study area.
Disparate Stone Age Technological Evolution in North China

The transition from Middle to Upper Paleolithic and the global diffusion of modern human populations remain hotly debated topics. The timing and pace of the transition in China are especially uncertain. This paper examines spatial and temporal variation among Paleolithic assemblages in North China dated to Marine Isotope Stage 3. There are two main systems of blank production in evidence: one is Levallois-like whereas the other involves simple unprepared flake cores. The Levallois-like assemblages are limited to northwest China: further dispersal of the technology was probably hindered by the presence of established populations in the eastern part of North China using long-established flake core technology. Consequently we should view North China as consisting of two geographic entities with respect to research on the Middle to Upper Paleolithic transition. Changes within flake assemblages represent an independent pattern of cultural evolution, and it will be important to clarify when and how other aspects of Upper Paleolithic behavior were expressed there.
The Changing Dynamics of Senate Voting on Supreme Court Nominees

A near-universal consensus exists that the nomination of Robert Bork in 1987 triggered a new regime in the Senate's voting over presidential nominees—a regime that deemphasizes ethics, competence, and integrity and stresses instead politics, philosophy, and ideology. Nonetheless, this conventional wisdom remains largely untested. In this paper we explore the extent to which the Bork nomination has affected the decisions of U.S. senators. To do so, we modernize, update, and backdate the standard account of confirmation politics offered by Cameron, Cover, and Segal (1990) to cover all candidates for the Supreme Court from Hugo L. Black in 1937 through John G. Roberts, Jr. in 2005. Our results confirm conventional wisdom about the Bork nomination but with two notable caveats. First, while the importance of ideology has reached new heights, the Senate's emphasis on this factor had its genesis some three decades earlier, in the 1950s. Second, while ideology is of paramount concern to senators, a candidate's professional merit also remains a significant determinant of success in the Senate.
Transalveolar maxillary sinus floor elevation using osteotomes with or without grafting material. Part II: Radiographic tissue remodeling.

OBJECTIVES To evaluate the pattern of tissue remodeling after maxillary sinus floor elevation using the transalveolar osteotome technique with or without utilizing grafting materials. METHODS During the period of 2000-2005, 252 Straumann dental implants were inserted using the transalveolar sinus floor elevation technique in a group of 181 patients. For 88 or 35% of those implants, deproteinized bovine bone mineral with a particle size of 0.25-1 mm was used as the grafting material, but for the remaining 164 implants, no grafting material was utilized. Periapical radiographs were obtained with a paralleling technique and digitized. Two investigators, who were blinded to whether grafting material was used or not, subsequently evaluated the pattern of tissue remodeling. RESULTS The mean residual bone height was 7.5 mm (SD 2.2 mm), ranging from 2 to 12.7 mm. The mean residual bone height for implants placed with grafting material (6.4 mm) was significantly less compared with the implants installed without grafting material (8.1 mm). The implants penetrated on average 3.1 mm (SD 1.7 mm) into the sinus cavity. The measured mean radiographic bone gain using the transalveolar technique without grafting material was significantly less, 1.7 mm (SD 2 mm) compared with a mean bone gain of 4.1 mm (SD 2.4 mm), when grafting material was used. Furthermore, the probability of gaining 2 mm or more of new bone was 39.1% when no grafting material was used. The probability increased to 77.9% when the implants were installed with grafting material. CONCLUSION When the transalveolar sinus floor elevation was performed without utilizing grafting material, only a moderate gain of new bone could be detected mesial and distal to the implants. On the other hand, when grafting material was used, a substantial gain of new bone was usually seen on the radiographs.
Health, welfare and fertility implications of the use of bovine somatotropin in dairy cattle

Twenty-two Friesian and Friesian cross Ayrshire cows and 16 first lactation heifers were paired. Twenty were injected subcutaneously with 500 mg recombinant bovine somatotropin (sometribove) and 18 with a placebo at fortnightly intervals, starting 80 (+/- 7) days after calving, through the winter of 1986-87. The cows' weights and changes in condition, milk yields, milk solids, health and fertility were recorded regularly. Compound cake was fed at a fixed stepped rate according to the number of days since the cow calved. Silage was available on an easy-feed basis. Ten cows had their daily silage intake measured. Sometribove treated cows produced on average 27.7 kg of milk per day, 4.5 kg more than the controls. Treated heifers produced 23.5 kg per day, 2.5 kg more than the controls. Milk quality was unchanged. Treated cows gained weight during the trial, but not by as much as the controls. Control heifers also gained weight but the treated heifers lost, on average, 3 kg. Local reactions at the injection site were not felt to be of welfare concern, nor was the general effect of the extra milk production. A small number of treated animals experienced mastitis and had poorer fertility but the differences were mostly not statistically significant. If bovine somatotropin should become licensed for use in Britain it is recommended that clear guidelines should be issued on the management practices necessary for economic success and for the welfare of the treated animals.
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract

Background Hydration and integrity of the stratum corneum (SC) is an important determinant of skin appearance, metabolism, mechanical properties, and barrier function. The presence of aquaglyceroporins and envelope proteins are crucial to provide greater corneocyte cohesion to keep water and other moisturizers in the skin.
THE THREE-DIMENSIONAL STRUCTURE OF INTERIOR EJECTA IN CASSIOPEIA A AT HIGH SPECTRAL RESOLUTION

We used the Spitzer Space Telescope's Infrared Spectrograph to create a high-resolution spectral map of the central region of the Cassiopeia A (Cas A) supernova remnant, allowing us to make a Doppler reconstruction of its three-dimensional structure. The ejecta responsible for this emission have not yet encountered the remnant's reverse shock or the circumstellar medium, making it an ideal laboratory for exploring the dynamics of the supernova explosion itself. We observe that the O, Si, and S ejecta can form both sheet-like structures and filaments. Si and O, which come from different nucleosynthetic layers of the star, are observed to be coincident in velocity space in some regions, and separated by 500 km s−1 or more in others. Ejecta traveling toward us are, on average, ∼900 km s−1 slower than the material traveling away from us. We compare our observations to recent supernova explosion models and find that no single model can simultaneously reproduce all the observed features. However, models of different supernova explosions can collectively produce the observed geometries and structures of the interior emission. We use the results from the models to address the conditions during the supernova explosion, concentrating on asymmetries in the shock structure. We also predict that the back surface of Cas A will begin brightening in ∼30 years, and the front surface in ∼100 years.
An Ancient Maya Reservoir and Dam at Tamarindito, El Peten, Guatemala

We report a Prehispanic dam and remnants of a small reservoir at the ancient Maya site of Tamarindito in the Río de la Pasión region of Guatemala's Petén district. The remnants of the dam are on the Petexbatún escarpment, to the east of the main site in the midst of an area of residential groups and numerous and varied agricultural terracing. The dam was about 60 m long and would have held a reservoir of about 2,000 m3. This volume of water was sufficient to irrigate nearby terraced fields. The dam and reservoir probably date to the Late Classic period (A.D. 550-800), a period during which Tamarindito was involved in regional warfare. In addition to producing irrigation water, we hypothesize that the system was built to provide a supplemental, more defensible, residential water supply and to protect a spring located below the dam.
Ultrasonographic Examination for Inversion Ankle Sprains Associated with Osseous Injuries

Hsu C-C, Tsai W-C, Chen CPC, Chen MJ-L, Tang SF-T, Shih L: Ultrasonographic examination for inversion ankle sprains associated with osseous injuries. Am J Phys Med Rehabil 2006;85:785–792. Objective:The study attempted to evaluate the value of ultrasonography in determining the therapeutic strategy for patients with osseous injuries caused by ankle sprains. Design:A 10-MHz compact linear-array ultrasound transducer was used to assess patients with inversion ankle sprains. Eleven female and 12 male patients who had fractures detected by sonograms were included in the study. All 23 patients underwent radiographic examination for identification of fractures. Bone scintigraphy was performed for those who had negative x-ray findings. Results:Eighteen patients had distal fibular tip fractures, three patients had the fifth metatarsal base fractures, one patient had a talar neck fracture, and one patient had a navicular fracture. These fractures were all detected by ultrasonography and then proved even by radiography or by bone scans. All the 23 patients had anterior talofibular ligament injuries. Among these patients, 11 had anterior ankle-joint recess effusion, and two had additional anterioinferior tibiofibular ligament injuries. Six weeks of immobilization with the ankle fracture brace was prescribed for all the patients after the identification of fractures. Their ankle pain symptoms soon improved. Conclusions:Ultrasonography is valuable in evaluating tiny foot and ankle fractures and coexistent soft tissue injuries. It can guide the treatment for patients with osseous injuries caused by ankle sprains.
Expression of HLA‐DR, CAM and co‐stimulatory molecules on cord blood monocytes

Abstract: Umbilical cord blood (CB) transplantations are associated with a lower risk of severe graft‐versus‐host disease (GVHD) compared to BMT. GVHD is an immune reaction that involves interaction between cell surface molecules resulting in cell activation and release of many cytokines. Monocytes are known to be an important source of cell adhesion (CAM) and co‐stimulatory molecules which play a crucial role in the efficient activation of T and B cells. We analyzed the phenotype of CB monocytes in the presence or absence of an inflammatory signal (rIFN‐γ) and compared them to adult blood (AB); the expression of HLA‐DR and 17 different markers (CD11a, CD11b, CD11c, CD18, CD29, CD40, CD44, CD49a, CD49d, CD49e, CD49f, CD54, CD58, CD62L, CD80, CD86 and CD102) was measured by flow cytometry. Statistical analysis showed that, compared to AB, CB monocytes did not express CD11b, CD11c, CD49d and after stimulation with rIFNγ, they lost the expression of CD58 and CD102, whereas CD80 and CD86 expression was induced. The analysis of fluorescence intensity (MFI) revealed that CB monocytes expressed some CAM (CD29, CD54, CD102) with a lower intensity than AB monocytes except CD44. In conclusion, absence and reduced expression of some markers argue for a different phenotypic profile of CB monocytes compared to AB monocytes, which might partly contribute to their impaired immune response and to the low incidence of GVHD observed after CB transplantations. However, CB monocytes expressed CD80 and CD86 co‐stimulatory molecules, but this expression did not prove a normal co‐stimulatory function.
Risk factors for fibular fracture after TPLO.

OBJECTIVE To determine (1) risk factors for fibular fracture after tibial plateau leveling osteotomy (TPLO) and (2) if a single postoperative radiographic measurement or measurement ratio of the proximal tibial fragment can be used as a predictor for fibular fracture. STUDY DESIGN Multivariate retrospective clinical study. ANIMALS Dogs (n=326) with cranial cruciate ligament rupture that had TPLO (n=355). METHODS Medical records (January 2004-November 2007) and radiographs of dogs that had TPLO were reviewed. TPLO plate type, distance between tibial plateau and proximal screw, proximodistal tibial plateau fragment length, tibial plateau width, the presence of a fibular drill hole filled with a screw or not, and fibular fractures were recorded. RESULTS The odds of having a fibular fracture were 10 times greater in dogs with a fibular drill hole than in dogs without a drill hole. The odds of having a fibular fracture were 1.46 times greater for every 4.5 g increase in body weight. Tibial plateau angle (TPA) at the time of reevaluation was larger than the postoperative TPA and TPA increase was larger in dogs with fibular drill holes than without (P<.01) and in dogs with fibular fractures than without (P<.01). CONCLUSION An unfilled fibular drill hole and increased body weight are risk factors for fibular fracture.
Interaction of longitudinal-optic phonons with free holes as evidenced in Raman spectra from Be-doped p-type GaAs.

Contrairement aux modes phonons LO-plasmons typiquement observes dans les semiconducteurs de type n de structure blende dans ces materiaux, seul un mode est observe pour les densites de trous entre 1×10 18 et 1,6×10 19 cm −3 . Ceci est attribue au suramortissement du systeme de trous lourds
Pharmacodynamics of once-daily amikacin in various combinations with cefepime, aztreonam, and ceftazidime against Pseudomonas aeruginosa in an in vitro infection model

The pharmacodynamics of once-daily amikacin administered as monotherapy and in combination with aztreonam, ceftazidime, and cefepime against Pseudomonas aeruginosa ATCC 27853 and clinical isolate 16690 (moderately susceptible to ceftazidime) were investigated with an in vitro model of infection over a 24-h period. Monotherapy with aztreonam, ceftazidime, and cefepime and combinations of aztreonam with cefepime or ceftazidime were also studied. MICs and MBCs were determined for viable organisms at 24 h to test for the development of resistance. Once-daily amikacin demonstrated killing activity over the initial 8 h superior to those of all other drugs administered as monotherapy against both strains tested (P < 0.01). Regrowth by 24 h was greatest for the amikacin regimen (P < 0.01) but was apparent for all monotherapy regimens against both strains. No changes in susceptibilities were observed. All combination therapies containing once-daily amikacin achieved 99.9% reductions in log10 CFU/ml by 2.0 h against both strains, with no regrowth of organisms at 24 h. Aztreonam-cefepime and -ceftazidime combinations required approximately 5 to 6 h to achieve a 99.9% reduction in log10 CFU/ml. Although there was no difference in time to the 99.9% kill between the aztreonam-cefepime and -ceftazidime regimens against either strain, the killing activity of these combinations was significantly less than those of regimens containing once-daily amikacin (P < 0.01). Minor differences in the initial susceptibilities of beta-lactams and the monobactam aztreonam against P. aeruginosa may not be important for therapeutic outcomes when used in combination with single-daily aminoglycoside therapy.