Miracle-dz/UniFAR-FTUs huggingface dataset:
Pediatric low-grade gliomas: implications of the biologic era

For the past decade, it has been recognized that pediatric low-grade gliomas (LGGs) and glial-neuronal tumors carry distinct molecular alterations with resultant aberrant intracellular signaling in the Ras-mitogen-activated protein kinase pathway. The conclusions and recommendations of a consensus conference of how best to integrate the growing body of molecular genetic information into tumor classifications and, more importantly, for future treatment of pediatric LGGs are summarized here. There is uniform agreement that molecular characterization must be incorporated into classification and is increasingly critical for appropriate management. Molecular-targeted therapies should be integrated expeditiously, but also carefully into the management of these tumors and success measured not only by radiographic responses or stability, but also by functional outcomes. These trials need to be carried out with the caveat that the long-term impact of molecularly targeted therapy on the developing nervous system, especially with long duration treatment, is essentially unknown.
The subpopulation of microglia sensitive to neurotransmitters/neurohormones is modulated by stimulation with LPS, interferon‐γ, and IL‐4

Recently, neurotransmitters/neurohormones have been identified as factors controlling the function of microglia, the immune competent cells of the central nervous system. In this study, we compared the responsiveness of microglia to neurotransmitters/neurohormones. We freshly isolated microglia from healthy adult C57Bl/6 mice and found that only a small fraction (1–20%) responded to the application of endothelin, histamine, substance P, serotonin, galanin, somatostatin, angiotensin II, vasopressin, neurotensin, dopamine, or nicotine. In cultured microglia from neonatal and adult mice, a similarly small population of cells responded to these neurotransmitters/neurohormones. To induce a proinflammatory phenotype, we applied lipopolysaccaride (LPS) or interferon‐gamma (IFN‐γ) to the cultures for 24 h. Several of the responding populations increased; however, there was no uniform pattern when comparing adult with neonatal microglia or LPS with IFN‐γ treatment. IL‐4 as an anti‐inflammatory substance increased the histamine‐, substance P‐, and somatostatin‐sensitive populations only in microglia from adult, but not in neonatal cells. We also found that the expression of different receptors was not strongly correlated, indicating that there are many different populations of microglia with a distinct set of receptors. Our results demonstrate that microglial cells are a heterogeneous population with respect to their sensitivity to neurotransmitters/neurohormones and that they are more responsive in defined activation states. GLIA 2014;62:667–679
Opposing shear senses in a subdetachment mylonite zone: Implications for core complex mechanics

Global studies of metamorphic core complexes and low‐angle detachment faults have highlighted a fundamental problem: Since detachments excise crustal section, the relationship between the mylonitic rocks in their footwalls and the brittle deformation in their hanging walls is commonly unclear. Mylonites could either reflect ductile deformation related to exhumation along the detachment fault, or they could be a more general feature of the extending middle crust that has been “captured” by the detachment. In the first case we would expect the kinematics of the mylonite zone to mirror the sense of movement on the detachment; in the second case both the direction and sense of shear in the mylonites could be different. The northern Snake Range décollement (NSRD) is a classic Basin and Range detachment fault with a well‐documented top‐east of displacement. We present structural, paleomagnetic, geochronological, and geothermometric evidence to suggest that the mylonite zone below the NSRD locally experienced phases of both east‐ and west‐directed shear, inconsistent with movement along a single detachment fault. We therefore propose that the footwall mylonites represent a predetachment discontinuity in the middle crust that separated localized deformation above from distributed crustal flow below (localized‐distributed transition (LDT)). The mylonites were subsequently captured by a moderately dipping brittle detachment that soled down to the middle crust and exhumed them around a rolling hinge into a subhorizontal orientation at the surface, producing the present‐day NSRD. In this interpretation the brittle hanging wall represents a series of rotated upper crustal normal faults, whereas the mylonitic footwall represents one or more exhumed middle crustal discontinuities (LDTs).
Anaerobic performance in 5- to 7-yr-old children of low birthweight.

PURPOSE This study was intended to determine whether anaerobic muscle performance is deficient in 5- to 7-yr-old children of extremely low birthweight (ELBW, 500-999 g) and very low birthweight (VLBW, 1000-1499 g). METHODS Fourteen ELBW and 20 VLBW children were compared with 24 normal birthweight (NBW, >2500 g) term controls. Peak (PP) and mean (MP) muscle power were determined by the Wingate anaerobic test. Bioimpedance analysis and anthropometry were done to assess fat-free mass (FFM) and lean cross-sectional area of the thigh and calf. RESULTS The ELBW group had significantly lower MP and PP, compared with the VLBW and, in particular, with the NBW group. This lower performance was apparent also when values were corrected for total body mass (MP) and FFM (MP and PP), but not when corrected for cross-sectional area of thigh and calf. CONCLUSION The lower anaerobic muscle performance in ELBW children may be partly due to their smaller muscle mass, but may also reflect a low percentage of fast-twitch muscle fibers, low muscle phosphagen content, or deficiency in motor control.
Effects of housing system (outdoor vs cages) and age of laying hens on egg characteristics

1. Effects of two housing systems (cages vs outdoor) on external and internal egg characteristics were investigated. 2. In total 785 eggs from three different lines in cages and 268 eggs from outdoor-housed layers were examined for egg weight, albumen, yolk and shell content, albumen height and pH, and albumen and yolk dry matter content. 3. Interactions between layer age and housing systems were found for egg weight, eggshell content, albumen height, albumen pH, and dry matter content of the albumen and yolk. This was mainly due to the greater variation with age in the outdoor layers, compared to the caged layers. 4. Irrespective of age eggs from outdoor layers were relatively broader than eggs from the caged layers. Yolk colour was considerably darker in the outdoor group (11.0 vs 9.3). 5. We concluded that it is more difficult to maintain constant external and internal egg quality in an outdoor housing system than in a battery cage system. Factors that determine the greater fluctuations in internal egg quality need to be investigated.
Water immersion in the treatment of exertional hyperthermia: physical determinants.

PURPOSE We examined the effect of differences in body surface area-to-lean body mass ratio (AD/LBM) on core temperature cooling rates during cold water immersion (CWI, 2°C) and temperate water immersion (TWI, 26°C) after exercise-induced hyperthermia. METHODS Twenty male participants were divided into two groups: high (315.6 ± 7.9 cm·kg, n = 10) and low (275.6 ± 8.6 cm·kg, n = 10) AD/LBM. On two separate occasions, participants ran on a treadmill in the heat (40.0°C, 20% relative humidity) wearing an impermeable rain suit until rectal temperature reached 40.0°C. After exercise, participants were immersed up to the nipples (arms remained out of the water) in either a CWI (2°C) or a TWI (26°C) circulated water bath until rectal temperature returned to 37.5°C. RESULTS Overall rectal cooling rates were significantly different between experimental groups (high vs low AD/LBM, P = 0.005) and between immersion conditions (CWI vs TWI, P < 0.001). Individuals with a high AD/LBM had an approximately 1.7-fold greater overall rectal cooling rate relative to those with low AD/LBM during both CWI (high: 0.27°C·min ± 0.10°C·min vs low: 0.16°C·min ± 0.10°C·min) and TWI (high: 0.10°C·min ± 0.05°C·min vs low: 0.06°C·min ± 0.02°C·min). Further, the overall rectal cooling rates during CWI were approximately 2.7-fold greater than during TWI for both the high (CWI: 0.27°C·min ± 0.10°C·min vs TWI: 0.10°C·min ± 0.05°C·min) and the low (CWI: 0.16°C·min ± 0.10°C·min vs TWI: 0.06°C·min ± 0.02°C·min) AD/LBM groups. CONCLUSION We show that individuals with a low AD/LBM have a reduced rectal cooling rate and take longer to cool than those with a high AD/LBM during both CWI and TWI. However, CWI provides the most effective cooling treatment irrespective of physical differences.
An ultra-low-power physics package for a chip-scale atomic clock

We report the design and measured thermal and mechanical performance of an ultra-low-power physics package for a chip-scale atomic clock (CSAC). This physics package enables communications and navigation systems that require a compact, low-power atomic frequency standard. The physics package includes a unique combination of thermal isolation, mechanical stability and robustness, and small package volume. We have demonstrated temperature control at a nominal operating temperature of 75/spl deg/C in a room-temperature, vacuum ambient requiring only 7mW of heating power. This represents a power reduction of over two orders of magnitude compared to the lowest-power existing commercial technology and more than an order of magnitude improvement over other CSAC development efforts.
Curriculum Learning and Minibatch Bucketing in Neural Machine Translation

We examine the effects of particular orderings of sentence pairs on the on-line training of neural machine translation (NMT). We focus on two types of such orderings: (1) ensuring that each minibatch contains sentences similar in some aspect and (2) gradual inclusion of some sentence types as the training progresses (so called “curriculum learning”). In our English-to-Czech experiments, the internal homogeneity of minibatches has no effect on the training but some of our “curricula” achieve a small improvement over the baseline.
Autoantibodies contribute to the immunopathogenesis of experimental dry eye disease.

PURPOSE The purpose of this study was to determine if autoantibodies play a role in the immunopathogenesis of experimental dry eye disease. METHODS Dry eye was induced by exposing female C57BL/6 wild-type mice or hen egg lysozyme B-cell receptor transgenic mice to desiccating stress (subcutaneous scopolamine [0.5 mg/0.2 mL] 3 times a day, humidity < 40%, and sustained airflow) for 3 weeks, allowing sufficient time for a humoral immune response. Serum or purified IgG isolated from dry-eye mice or untreated controls was passively transferred to nude recipient mice, which were evaluated for ocular surface inflammation 3 days after transfer. To determine if complement activation contributed to serum-induced dry eye disease, cobra venom factor was used to deplete complement activity. RESULTS Autoantibodies against kallikrein 13 were identified in serum from dry-eye mice, but were undetectable in untreated controls. Autoantibody-containing serum or purified IgG from dry-eye mice was sufficient to mediate complement-dependent ocular surface inflammation. Serum or purified IgG caused marked inflammatory burden and tissue damage within the ocular surface tissues, including elevated Gr1+ neutrophil infiltration and proinflammatory cytokines/chemokines associated with goblet cell loss. Moreover, complement C3b deposition was found within the ocular surface tissues of mice receiving dry-eye serum, but not in recipients of control serum. Functionally, complement depletion attenuated the ability to transfer dry-eye-specific serum or IgG-mediated disease. CONCLUSIONS These data demonstrate for the first time a complement-dependent pathogenic role of dry-eye-specific autoantibodies, and suggest autoantibody deposition within the ocular surface tissues contributes to the predominantly T-cell-mediated immunopathogenesis of dry eye disease.
Expression Pattern of Prokineticin 1 and Its Receptors in Bovine Ovaries During the Estrous Cycle: Involvement in Corpus Luteum Regression and Follicular Atresia

Abstract Prokineticin 1 (PROK1), also termed endocrine gland-derived vascular endothelial growth factor (endocrine gland-derived VEGF), is a newly identified protein assigned with diverse biologic functions. It binds two homologous G protein-coupled receptors, PROKR1 and PROKR2. To better understand the roles of PROK1 and its receptors in ovarian function, their expression was determined in follicles and corpora lutea (CLs) at different developmental stages. PROK1 mRNA levels were low at early luteal stage and midluteal stage, but increased sharply during natural or induced luteolysis. High PROK1 mRNA levels also were found in atretic follicles. This profile of PROK1 expression was opposite to that of the well-established angiogenic factor VEGF. Of the two receptor-type expressions, PROKR1 but not PROKR2 was correlated positively with its ligand. Immunohistochemical staining revealed that PROK1 was located mainly within the muscular layer of arterioles, and during regression it also was localized to macrophages and steroidogenic cells. The expression pattern of ITGB2 mRNA, a leukocyte cell marker, overlapped that of PROK1, thus suggesting that leukocyte infiltration may explain the elevated expression of PROK1 in atretic follicles and regressing CL. Indeed, flow cytometry analyses showed that nearly all beta-2 integrin chain (ITGB2)-positive cells also were stained with anti-PROK1 and that significantly more ITGB2/PROK1 double-stained cells were present in degenerating follicles and CL. Furthermore, when challenged in vitro with PROK1, adherent, mononuclear cell numbers and TNF levels were elevated, indicating that PROK1 triggers monocyte activation. Together, these data suggest that PROK1, acting via PROKR1, may be involved in the recruitment of monocytes to regressing CL and atretic follicles and their consequent activation therein.
Interacting vortices and spin-up in two-dimensional turbulence.

In simulations of decaying two-dimensional turbulence, Clercx and co-workers discovered the unexpected phenomenon of "spin-up. " (This is the spontaneous acquisition of angular momentum by a turbulent two-dimensional fluid in a rigid container.) Here we show that this phenomenon can readily be interpreted in terms of statistical models of two-dimensional turbulence. When the net vorticity is zero in a bounded system, there are two distinct types of statistical equilibrium. The first has the expected property that its angular momentum is zero. However, the second type has a nonzero angular momentum even though its circulation vanishes. The relative probability of the two types of equilibrium depends strongly on the shape of the boundary and weakly on the energy. The angular momentum predicted for the second type of equilibrium is in good agreement with that found in simulations at high Reynolds number.
Interplay Between N-Terminal Methionine Excision and FtsH Protease Is Essential for Normal Chloroplast Development and Function in Arabidopsis[C][W]

This study reports on the tight functional interplay between cotranslational N-terminal methionine excision (NME) and the thylakoid FtsH protease complex, highlighting that correctly NME-processed N-termini of D1 and D2 polypeptides of photosystem II are indispensable for both FtsH-mediated protein quality control and repair-related degradation of D1 and D2. N-terminal methionine excision (NME) is the earliest modification affecting most proteins. All compartments in which protein synthesis occurs contain dedicated NME machinery. Developmental defects induced in Arabidopsis thaliana by NME inhibition are accompanied by increased proteolysis. Although increasing evidence supports a connection between NME and protein degradation, the identity of the proteases involved remains unknown. Here we report that chloroplastic NME (cNME) acts upstream of the FtsH protease complex. Developmental defects and higher sensitivity to photoinhibition associated with the ftsh2 mutation were abolished when cNME was inhibited. Moreover, the accumulation of D1 and D2 proteins of the photosystem II reaction center was always dependent on the prior action of cNME. Under standard light conditions, inhibition of chloroplast translation induced accumulation of correctly NME-processed D1 and D2 in a ftsh2 background, implying that the latter is involved in protein quality control, and that correctly NME-processed D1 and D2 are turned over primarily by the thylakoid FtsH protease complex. By contrast, inhibition of cNME compromises the specific N-terminal recognition of D1 and D2 by the FtsH complex, whereas the unprocessed forms are recognized by other proteases. Our results highlight the tight functional interplay between NME and the FtsH protease complex in the chloroplast.
Fast online action recognition with efficient structured boosting

In this paper, we propose a novel robust action recognition framework with the following capabilities: 1) online encoding motions to multi-label sequence where the output in each frame is a tuple of labels rather than a single label, 2) providing efficient automatic relevant motion selection framework, 3) learning systems so as to be optimal for onlinemultilabel sequence classification. As for multi-label classification, our approach incorporates contextual information about action not only temporal information but hierarchical information of actions. Inference tends to be complex so as to achieve such complex recognition scheme, however, we propose an efficient Viterbi-like decoding algorithm which integrates forward algorithm and loopy message passing algorithm. As for the learning process, the algorithm optimizes the parameters so as to maximize log likelihood of the model. Boosting, ensemble approach of machine learning, is leveraged to provide efficient feature selection framework in the training process. The experimental results show that the proposed method successfully exploits the impact of contextual information then significantly outperforms the traditional approaches in dynamic gait motion classification.
Efficacy of chlorhexidine varnish applications in the prevention of early childhood caries.

AIM A prospective clinical study was conducted to evaluate the influence of 3-monthly applications of the chlorhexidine- containing varnish Cervitec on the colonisation of a child's oral cavity by mutans streptococci (MS) and on caries prevalence. METHODS 200 children aged 11.7 +/- 0.7 months were examined. Children's caries risk was assessed on the basis of their MS scores in saliva. Out of the 48 children in whom a high caries risk (> or =10(5) CFU/ml) was expected, 23 were treated with Cervitec at 3-month intervals (CHX group). The remaining 25 children of the high-risk group received no treatment and served as controls (group C). All parents received detailed information on the prevention of early childhood caries. 172 children had completed the study after one year. RESULTS During the course of the study the percentage of children with visible plaque on their maxillary incisors increased from 17.8% to 40.1% and the percentage of children given sweetened drinks in nursing bottles for the night rose from 16.3% to 18.0%. At the closure of the study 26.2% of the two-year-olds had salivary scores of MS > or =10(5) CFU/ml of saliva. The mean d(1-4)mft value increased from 0.05 -/+ 0.4 to 0.8 -/+ 2.9 and the mean d(1-4)mfs value rose from 0.08 -/+ 0.8 to 1.8 -/+ 5.9. No significant differences were demonstrable between the two-year-olds in groups CHX and C for colonisation of the oral cavity by MS or for d(1-4)mft values. In contrast the d(1-4)mfs values were significant lower in the CHX group as in the group C. CONCLUSION Poor feeding habits and deficits in oral hygiene cannot be compensated by the application of Cervitec.
The biological limitations of transcriptomics in elucidating stress and stress responses

Global analysis of mRNA abundance via genomic arrays (i.e. transcriptomics or transcriptional profiling) is one approach to finding the genes that matter to organisms undergoing environmental stress. In evolutionary analyses of stress, mRNA abundance is often invoked as a proxy for the protein activity that may underlie variation in fitness. To provoke discussion of the utility and sensible application of this valuable approach, this manuscript examines the adequacy of mRNA abundance as a proxy for protein activity, fitness and stress. Published work to date suggests that mRNA abundance typically provides little information on protein activity and fitness and cannot substitute for detailed functional and ecological analyses of candidate genes. While the transcriptional profile can be an exquisitely sensitive indicator of stress, simpler indicators will often suffice. In view of this outcome, transcriptomics should undergo careful cost‐benefit analysis before investigators deploy it in studies of stress responses and their evolution.
Socio-economic status, standard of living, and neurotic disorder

Evidence on the association between socio-economic status and the prevalence of neurotic disorder is contradictory. We studied the association between three elements of socio-economic status and the prevalence of neurotic psychiatric disorder in a representative sample of adults aged 16-64 living in private households in the UK. A cross-sectional survey of 10,108 adults aged 16-65 resident in private households in the UK was selected by a multi-stage, clustered, random-sampling design. Neurotic disorders were defined using a standardised interview, the revised clinical interview schedule (CIS-R). Data for 9570 people were available for this study. We used housing tenure and access to cars as measures of standard of living; both were associated with the prevalence of neurotic disorder even after adjustment for other socio-economic and demographic variables, including the Registrar General's Social Class and educational attainment. Those people with no access to a car had an odds ratio for neurotic disorder of 1.4 (95% CI 1.1-1.7), compared with those who had access to two or more cars. People who rented their homes were also at increased risk (1.3 [1.1-1.5]). We estimated that about 10% of the neurotic disorder in the UK could be attributed to the increased prevalence of those without cars who rented their homes. There was a complex interaction between the Registrar General's Social Class and sex, and there was no independent association with educational attainment. There is an independent association between low standard of living and the prevalence of neurotic psychiatric disorder. The UK has experienced one of the largest increases in income inequality within western market economies over the past 20 years, and this inequality may have had adverse consequences for the mental health of the population.