Miracle-dz/UniFAR-FTUs huggingface dataset:
Classical Swine Fever Virus p 7 protein is a viroporin involved in virulence in swine 1 2

28 The non-structural protein p7 of Classical Swine Fever Virus (CSFV) is a small 29 hydrophobic polypeptide with an apparent molecular mass of 6 to 7 kDa. The protein 30 contains two hydrophobic stretches of amino acids interrupted by a short charged 31 segment that are predicted to form transmembrane helices and a cytosolic loop 32 respectively. Using reverse genetics, partial in-frame deletions of p7 were deleterious for 33 virus growth, demonstrating that CSFV p7 function is critical for virus production in cell 34 cultures. A panel of CSFV recombinant mutant viruses was created using alanine 35 scanning mutagenesis of the p7 gene harboring sequential three to six amino acid 36 residues long substitutions spanning the entire protein. These recombinant viruses 37 allowed the identification of the regions within p7 that are critical for virus production in 38 vitro. In vivo, some of these viruses were partially or completely attenuated in swine 39 relative to the highly virulent parental CSFV Brescia strain indicating a significant role of 40 p7 in CSFV virulence. Structure-function analyses in model membranes emulating the 41 ER lipid composition confirmed that CSFV p7 is a pore forming protein, and that pore42 forming activity resides in the C-terminal transmembrane helix. Therefore, p7 is a 43 viroporin which is clearly involved in the process of CSFV virulence in swine. 44 45 on July 0, 2017 by gest http/jvi.asm . rg/ D ow nladed fom
Numerical Activities of Daily Living – Financial (NADL-F): A tool for the assessment of financial capacities‡

ABSTRACT Financial capacity is the ability to manage one’s own finances according to self-interests. Failure in financial decisions and lack of independence when dealing with money can affect people’s quality of life and are associated with neuropsychological deficits or clinical conditions such as mild cognitive impairment or Alzheimer’s disease. Despite the importance of evaluating financial capacity in the assessment of patients with neuropsychological and psychiatric disorders, only a few tools have been developed. In the present article, the authors introduce the Numerical Activities of Daily Living – Financial (NADL-F) test, a new test to assess financial capacity in clinical populations. The NADL-F is relatively short, yet it encompasses the most common activities involving financial capacities. The NADL-F proved to have satisfactory psychometric properties and overall good validity for measuring financial abilities. Associations with performance on basic neuropsychological tests were investigated, in particular focusing on mathematical abilities as cognitive correlates of financial capacity. Results indicate that the NADL-F could be a useful tool to guide treatments for the enhancement of financial capacities. By sharing all materials and procedures, the authors hope to promote the development of further versions of the NADL-F in different languages, taking into account the necessary adjustments related to different socio-cultural contexts.
R plasmids in environmental Vibrio cholerae non-O1 strains

The occurrence of drug resistance and its plasmid-mediated transferability was investigated in 140 environmental strains of Vibrio cholerae non-O1 and 6 strains of Vibrio cholerae, both O1 and non-O1, of clinical origin. Of the 146 strains tested, 93% were resistant to at least one drug and 74% were resistant to two or more antibiotics. The O1 strains were susceptible to all antibiotics used. A total of 26 of 28 selected resistant wild strains carried R plasmids that were transferable by intraspecific and intergeneric matings. The most common transmissible R factor determined resistance to ampicillin, amoxicillin, and sulfanilamide (30%), followed by resistance to ampicillin and amoxicillin (13%) and resistance to ampicillin, amoxicillin, phosphomycin, and sulfanilamide (9%). Comparison of the three methods of plasmid analysis showed that the method of Birnboim and Doly (Nucleic Acids Res. 7:1513-1523, 1979) without EDTA and lysozyme was optimal for isolation of both large and small plasmids in environmental V. cholerae strains. Most strains harbored more than one plasmid, and the molecular sizes ranged from 1.1 to 74.8 megadaltons. The plasmids of high molecular size (around 74 megadaltons) were responsible for the resistance pattern transferred and were maintained with high stability in the hosts.
Rheumatoid factor by laser nephelometry and Waaler–Rose assay: prognostic value in patients with recent‐onset rheumatoid arthritis

Objective: To evaluate the prognostic value of rheumatoid factor (RF), detected in the Waaler–Rose agglutination assay and by nephelometry, in patients with recent‐onset rheumatoid arthritis (RA). Methods: Consecutive patients with new‐onset RA between 1993 and 1997 were followed for a median period of 4.7 years. Clinical data at baseline and drug use during the disease course were recorded. Outcome parameters studied were disease process, damage (erosions, joint surgery, extra‐articular manifestations, and new co‐morbidity), and death. Cut‐off levels for RF were >40 IU/mL (nephelometry) and titres ⩾1:160 (Waaler–Rose haemagglutination). Results: RF tests were negative by both methods in 22% of RA patients (RF− group), while 33% were RF positive by nephelometry only (RF+ group) and 45% were positive by Waaler–Rose and nephelometry (RF++ group). Baseline clinical and laboratory findings as well as the number of subsequently used disease‐modifying anti‐rheumatic drugs (DMARDs), the number of patients starting and the time spent on steroid therapy were similar in the three RF groups. Odd ratios for death (n = 23), erosions (n = 62), and serious extra‐articular disease manifestations (EAMs) (n = 13) as well as patient survival, erosion‐free or surgery‐free survival rates did not differ between the RF groups. Only rheumatoid nodules were more frequent in RF++ patients. Conclusion: The baseline presence of RF by either Waaler–Rose or nephelometry was not associated with differences in drug therapy, morbidity other than rheumatoid nodules, or mortality in RA patients in the first 5 years of disease. Being immunoglobulin M (IgM) RF positive thus had little impact on RA patient outcome.
Early phenotype expression of cortical neurons: evidence that a subclass of migrating neurons have callosal axons.

The use of [3H]thymidine labeling in combination with various axonal transport tracers has revealed that a subset of migrating neurons in the fetal monkey cerebrum issue axons to the opposite cerebral hemisphere while still migrating to their final positions in the cortical plate. Other cortical neurons with the same "birthdate" (i.e., that underwent their last round of DNA synthesis on the same day) are not retrogradely labeled by tracer injections of the opposite hemisphere. These findings suggest that the cardinal distinction between projection and local circuit neurons may be specified in postmitotic neurons before they acquire their final positions in the cortex.
Economic incentives on DNSSEC deployment: Time to move from quantity to quality

The security extensions to the DNS (DNSSEC) currently cover approximately 3% of all domains worldwide. In response to the low deployment of DNSSEC, a few top-level domains started offering 'per-domain' economic incentives to encourage adoption of the protocol by offering a yearly discount on each signed domain. However, it remains unclear whether these incentives are well-balanced and foster the overall security of the infrastructure as well as its deployment at scale. In this paper we argue that, in the presence of fixed costs of deployment, misaligned 'per-domain' incentives may have the collateral effect of encouraging large operators to massively deploy unsecure implementations of DNSSEC, whereas smaller operators, for which the effect of the economic incentive is negli­gible, may not significantly benefit from it. To investigate this, we study the security of DNSSEC deployment at scale, particularly in TLDs that offer economic incentives. We find that the security of DNSSEC implementations in the wild poorly reflects standard recommendations, particularly for tasks that cannot be solved by triggering a flag in the DNS software service (e.g. key rollover). Further, we find that, on average, large operators deploy weak DNSSEC security more frequently than small DNSSEC operators, suggesting that current incentives are ineffective in promoting a secure adoption and in deterring insecure implementations. We conclude the paper with actionable recommendations for TLD registry operators to improve the alignment of economic incentives with secure DNSSEC requirements.
Subtypes of Smokers who Attend Psychological Treatment in Order to Stop Smoking

The aim of this study is to identify subtypes of smokers who attended psychological treatment during the period 2006–2008 for smoking cessation from a sample of current smokers (N = 202) from Galicia, Spain. Eight instruments were used for data collection. Demographic variables, smoking variables, and psychopathological characteristics were clustered to establish typologies of smokers. A two-phase cluster analysis identified two subtypes of smokers (141 in Cluster 1 and 61 in Cluster 2). Those in Cluster 2 were younger, and had made an attempt to quit in the last year. They presented higher levels of nicotine dependence and psychopathology than those in Cluster 1. Differences in nicotine withdrawal syndrome, cigarette consumption at the end of the treatment, and adherence to the treatment were significant across the two clusters.
Characterization of the basal temporal language area in patients with left temporal lobe epilepsy

We evaluated 5 consecutive patients with subdural grid electrodes (including placement over the left basal temporal region) for focal resections for control of intractable epilepsy. All 5 had language dysfunction when we performed cortical stimulation over the basal temporal region (the inferior temporal gyrus, the fusiform gyrus, or the parahippocampal gyrus) using a systematic battery of language tests. The area in which language interference could be produced began from at least 11 to 35 mm posterior to the temporal tip and ended at least 39 to 74 mm posterior to the temporal tip. The most consistently impaired language tasks were spontaneous speech and passage reading, but there was impairment of all language functions tested in some patients. Language deficits after dominant temporal lobectomy may result from resection of this area.
Green Marketing : A Study of Consumers ’ Attitude towards Environment Friendly Products

-Concerns regarding the preservation of environment are increasingly becoming evident in the ecoconscious market places. This has resulted in the emergence of green marketing concept which has been adopted by developed countries long ago, but recently developing countries like Pakistan have awakened to green movement as well. This study investigates the consumers’ attitude towards environment friendly products and its’ impact on consumers’ purchases intentions. It was conducted in the third largest city and industrial hub of Pakistan i.e. Faisalabad, where due to heavy industrial activities and large population, environmental issues have become the persistent public concerns. The primary focus of this research is to investigate whether the green marketing influences consumer’s buying behavior or not. Furthermore the factors that motivate green-buying attitude among the consumers are examined and major challenges/ problems confronted by the consumers are identified. A survey is conducted from a sample of 150 respondents selected through convenience sampling technique. The data is analyzed by employing descriptive statistics and regression analysis. The results of the study show that consumers’ attitude significantly influences consumer’s green buying intentions along with other major variables like eco-literacy, values, perceptions and behavior. It is found that there is great potential for environment friendly products in Pakistan consumers, as considerable number of respondents tends to be collectivistic and ecologically conscious that ensures more chances of their engagement in pro-environmental behavior and hence influencing their green purchase intentions.
High-Efficiency Contactless Power Transfer System for Electric Vehicle Battery Charging Application

In this paper, a contactless charging system for an electric vehicle (EV) battery is proposed. The system consists of three parts: 1) a high-frequency power supply from a full-bridge inverter with frequency modulation; 2) a loosely coupled transformer that utilizes series resonant capacitors for both the primary and secondary windings; and 3) a rectification output circuit that uses a full-bridge diode rectifier. With carefully selected compensation network parameters, zero-voltage switching can be ensured for all the primary switches within the full range of an EV battery charging procedure, which allows the use of low ON-state resistance power MOSFETs to achieve high-frequency operation and system efficiency. The design of loosely coupled transformer is simulated and verified by finite element analysis software. For a 4-kW hardware prototype, the peak dc-dc efficiency reaches 98% and 96.6% under 4- and 8-cm air gap conditions, respectively. The prototype was tested with an electronic load and a home-modified EV to verify the performance of constant current and constant voltage control and their transitions.
Generation of dual-wavelength picosecond pulses with close wavelength separation from a self-seeded Fabry-Perot laser diode

We present a simple and low-cost technique for the generation of dual-wavelength picosecond laser pulses with a close wavelength separation. The technique is based on the selection of two close wavelengths from one of the modes of a self-seeded Fabry-Perot laser diode (FP-LD) with a fiber Bragg grating (FBG) that has a slightly split reflection spectrum. With this technique, the intensities of the pulses at the two wavelengths can be equalized by adjusting the temperature of the FP-LD, and the wavelength separation can be changed continuously without changing the repetition rate of the output pulses by tuning the separation of the reflection peaks of the FBG. Generation of stable dual-wavelength pulses with a pulsewidth of 36.0-50.0 ps and a tunable wavelength separation from 0.21 to 0.44 nm at a pulse rate of 2 GHz was demonstrated.
Echinococcus vogeli Rausch and Bernstein, 1972, from the paca, Cuniculus paca L. (Rodentia: Dasyproctidae), in the Departamento de Santa Cruz, Bolivia.

Among approximately 2,000 mammals examined for helminths in various regions of Bolivia during 1983-1987, cysts of Echinococcus vogeli Rausch and Bernstein, 1972, were found in a single paca, Cuniculus paca L. , collected at La Laguna, Departamento de Santa Cruz (lat. 16 degrees 36'W; long. 62 degrees 42'S). This record, the first from Bolivia, represents a considerable extension of the known geographic range of this species in South America. Upon analysis of the morphologic characteristics of the protoscoleces derived from the cysts, the sizes of rostellar hooks from the material from the paca were found to be well within the ranges reported in previous studies. Statistical analysis of frequency distributions of hook characteristics revealed some deviations from normality. These results indicate that parametric statistics should be applied with caution in analyses of inter-and intraspecific variation of morphologic characteristics of hooks of metacestodes of the genus Echinococcus.
Evaluations of bacterial contaminated full thickness burn wound healing in Sprague Dawley rats Treated with Tualang honey

Aim: The effect of Tualang honey on wound healing in bacterial contaminated full-thickness burn wounds was evaluated in 36 male Sprague Dawley rats. Materials and Methods: The rats were randomly divided into three groups (n = 12/group). Three full-thickness burn wounds were created on each rat. Each group of rats was inoculated with a different organism in the burn wounds: Group A was inoculated with Pseudomonas aeruginosa, Group B was inoculated with Klebsiella pneumoniae and Group C was inoculated with Acinetobacter baumannii. One wound on each rat was dressed with either Tualang honey, Chitosan gel or Hydrofibre silver. Each wound size was measured on day 3, 6, 9, 12, 15, 18 and 21 of the study. Results: The mean wound size of the Tualang honey-treated wounds was not statistically different than that of the Chitosan gel or Hydrofibre silver-treated wounds when the wounds were compared throughout the entire experiment (P > 0.05). However, comparing the mean wound size on day 21 alone revealed that the Tualang honey-treated wounds were smaller in comparison to that of the Chitosan gel and Hydrofibre silver-treated groups. Conclusions: This study shows that topical application of Tualang honey on burn wounds contaminated with P. aeruginosa and A. baumannii gave the fastest rate of healing compared with other treatments.
Restless roosts: Light pollution affects behavior, sleep, and physiology in a free‐living songbird

The natural nighttime environment is increasingly polluted by artificial light. Several studies have linked artificial light at night to negative impacts on human health. In free‐living animals, light pollution is associated with changes in circadian, reproductive, and social behavior, but whether these animals also suffer from physiologic costs remains unknown. To fill this gap, we made use of a unique network of field sites which are either completely unlit (control), or are artificially illuminated with white, green, or red light. We monitored nighttime activity of adult great tits, Parus major, and related this activity to within‐individual changes in physiologic indices. Because altered nighttime activity as a result of light pollution may affect health and well‐being, we measured oxalic acid concentrations as a biomarker for sleep restriction, acute phase protein concentrations and malaria infection as indices of immune function, and telomere lengths as an overall measure of metabolic costs. Compared to other treatments, individuals roosting in the white light were much more active at night. In these individuals, oxalic acid decreased over the course of the study. We also found that individuals roosting in the white light treatment had a higher probability of malaria infection. Our results indicate that white light at night increases nighttime activity levels and sleep debt and affects disease dynamics in a free‐living songbird. Our study offers the first evidence of detrimental effects of light pollution on the health of free‐ranging wild animals.
Therapeutic role of quercetin on oxidative damage induced by acrylamide in rat brain

Abstract Context Quercetin (QE), a bioflavonoid present abundantly in fruits and vegetables, has been reported to possess antioxidant properties. Acrylamide (ACR) is formed in foods during cooking and is known to be neurotoxic. Objective The present study was designed to evaluate the protective effect of QE against neurotoxicity induced by ACR. Materials and methods Four groups of Wistar rats consisting of six rats each: (i) control group; (ii) acrylamide treated group (50 mg/kg body weight as single dose); (iii) quercetin group: rats were treated intraperitoneally (i.p.) with QE (10 mg/kg body weight alone every day for 5 d); (iv) quercetin + acrylamide group: quercetin (10 mg/kg bw) was given i.p. every day for 5 d followed by acrylamide i.p. injection (50 mg/kg bw) on fifth day (single dose). Rats were killed after 48 h. Results Administration of ACR (50 mg/kg bw) in Wistar rats resulted in significant increase of dopamine, interferon-γ and 8-hydroxyguanosine with concomitant decrease of serotonin (p < 0.001) in the rat brain. Treatment of rats with QE intraperitonealy (10 mg/kg body weight) before ACR assault resulted in the diminution of ACR-mediated neurotoxicity as evident from decreased levels of dopamine, interferon-γ (p < 0.001) and 8-hydroxyguanosine with concomitant restoration of serotonin levels (p < 0.001). Discussion and conclusion On the basis of the above results, the present study suggests that quercetin may be a potential therapeutic agent for restoration of oxidative damage to neurons.
Kindler syndrome in mice and men

Kindler syndrome (KS) in humans is a severe skin blistering disease associated with inflammation and increased risk of epidermal squamous cell carcinoma (SCC). This disease is known to be caused by loss-of-function mutations in Kindlin-1, a focal adhesion β-integrin binding protein. Thus far, it has been unclear what specific signaling events occur in KS keratinocytes to promote tumorigenesis, especially since loss of β-integrins and focal adhesion complexes has been previously shown to prevent or delay tumor formation. In the April issue of Nature Medicine, Rognoni and colleagues generate a transgenic mouse lacking Kindlin-1 in the epidermis to model the key features of KS, and show that Kindlin-1 regulates Wnt and TGFβ signaling independent of β-integrins. These β1-integrin-independent functions of Kindlin-1 may contribute to the increased SCC risk in KS patients.
Confronting environmental racism : voices from the grassroots

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Anesthetic practice and pregnancy. Controlled survey of male anaesthetists in the United Kingdom.

7949 male doctors in the U.K. were surveyed to determine whether there was a relation between operating-theatre work (exposure) and abnormalities in the obstetric history of their marriages. Paternal exposure did not appear to influence the overall abortion-rate or the frequency of major congenital abnormality and involuntary infertility. The frequency of minor congenital abnormality in the children of exposed fathers was 3.09% compared with 2.35% (non-exposed). Maternal exposure was associated with a 15.5% frequency of abortion. Matching of exposed and non-exposed pregnancies with respect to birth order, maternal age, and smoking-habits suggests that the risk of abortion may increase by 158% to 271% with maternal exposure.
Trace elements in human health and disease.

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