Miracle-dz/UniFAR-FTUs huggingface dataset:
Highlights of regional meeting of Italian Southern Oncological Group (GOIM): focus on hepatocellular carcinoma: biological and clinical background, therapeutic guide-lines and perspectives

This Italian meeting, co-sponsored by the Italian Southern Oncological Group (GOIM), focused on different aspects of hepatocellular carcinoma (HCC). Topics included epidemiological studies, identification of environmental and genetic risk factors, the molecular mechanisms of pathogenesis, new diagnostic approaches and standard and novel treatment modalities. HCC remains a major health problem worldwide. It is the fifth most common cancer in the world, and the third most common cause of cancer-related death. The goal of HCC management is ‘cancer control’, reduction in its incidence and mortality as well as improvement in the quality of life of patients with HCC and their families. HCC epidemiology in Campania and the role of hepatitis viruses C and B in the pathogenesis of this cancer were described. Subsequently, state of the art and perspectives on the transduction pathways as therapeutic targets in HCC were evaluated. Another session was about HCC diagnosis by imaging techniques. The last session was dedicated to clinical treatment of this cancer, the role of liver transplantation, surgical resection, loco-regional procedures and systemic treatment modalities. Finally, the final results of the So.Lar. study on the Sorafenib + octreotide long-acting release (LAR) combination were presented.
Medical abortion by mifepristone with sublingual vs vaginal misoprostol: A randomised comparative trial

To compare the success and side effects between the sublingual and the vaginal administration of misoprostol for induced abortion in women treated initially with mifepristone. We administered 200 mg of mifepristone to 117 women who requested termination of pregnancy within 9 weeks duration. Forty eight hours later they were randomized into two groups. Fifty eight women received two doses of misoprostol sublingually 3 hrs apart while 59 women received misoprostol 800 mcg vaginally. They were observed for three hours and returned on day 15 for final assessment. Complete abortion occurred in 96.5% (95% CI: 91-100) of women in the sublingual group and 96.6% (95% CI: 91-100) in the vaginal group. This regimen resulted in complete abortion in all women (100%) when it was administered before 7 weeks gestation. The median induction-to-abortion interval was shorter in women in the sublingual group compared to the vaginal group (P <0.001). Women in the sublingual group experienced more diarrhea (P = 0.005) chills (P <0.001) and fever (P = 0.001). Two doses of sublingual misoprostol administered after mifepristone is an effective alternative route to the standard vaginal administration for medical abortion.
Fixed‐dose combination ezetimibe+atorvastatin lowers LDL‐C equivalent to co‐administered components in randomized trials: use of a dose–response model

Co‐administration of ezetimibe with atorvastatin is a generally well‐tolerated treatment option that reduces LDL‐C levels and improves other lipids with greater efficacy than doubling the atorvastatin dose. The objective of the study was to demonstrate the equivalent lipid‐modifying efficacy of fixed‐dose combination (FDC) ezetimibe/atorvastatin compared with the component agents co‐administered individually in support of regulatory filing. Two randomized, 6‐week, double‐blind cross‐over trials compared the lipid‐modifying efficacy of ezetimibe/atorvastatin 10/20 mg (n = 353) or 10/40 mg (n = 280) vs. separate co‐administration of ezetimibe 10 mg plus atorvastatin 20 mg (n = 346) or 40 mg (n = 280), respectively, in hypercholesterolemic patients. Percent changes from baseline in LDL‐C (primary endpoint) and other lipids (secondary endpoints) were assessed by analysis of covariance; triglycerides were evaluated by longitudinal‐data analysis. Expected differences between FDC and the corresponding co‐administered doses were predicted from a dose–response relationship model; sample size was estimated given the expected difference and equivalence margins (±4%). LDL‐C‐lowering equivalence was based on 97.5% expanded confidence intervals (CI) for the difference contained within the margins; equivalence margins for other lipids were not prespecified. Ezetimibe/atorvastatin FDC 10/20 mg was equivalent to co‐administered ezetimibe+atorvastatin 20 mg in reducing LDL‐C levels (54.0% vs. 53.8%) as was FDC 10/40 mg and ezetimibe+atorvastatin 40 mg (58.9% vs. 58.7%), as predicted by the model. Changes in other lipids were consistent with equivalence (97.5% expanded CIs <±3%, included 0); triglyceride changes varied more. All treatments were generally well tolerated. Hypercholesterolemic patients administered ezetimibe/atorvastatin 10/20 and 10/40 mg FDC had equivalent LDL‐C lowering. This FDC formulation proved to be an efficacious and generally well‐tolerated lipid‐lowering therapy.
EVALUATION OF CLINICAL BIOCHEMISTRY OF BLOOD IN BITCHES AFFECTED WITH PYOMETRA

Nine pyometric and eight healthy bitches were included to evaluate their plasma biochemical, enzymatic and sex steroid profile before and after ovario-hysterectomy. The pre-operative levels of plasma urea nitrogen (PUN), creatinine and total cholesterol were significantly (P<0.01) higher in pyometric bitches than the normal ones (60.78±10.03 vs 16.75±1.58 mg/dl; 2.71±0.51 vs 0.85±0.10 mg/dl and 256.52±25.19 vs 189.00±8.91 mg/dl, respectively). The post-operative levels of all three constituents, particularly PUN and creatinine, declined in cases of pyometra. Plasma total protein (7.67±0.61 vs 5.70±0.28 g/dl) and globulin (5.29±0.41 vs 2.30±0.13 g/dl) concentrations were significantly (P<0.01) higher and albumin (2.38±0.22 vs 3.40±0.28 g/dl) concentration was lower in pyometric bitches than the healthy ones, thus significantly (P<0.01) reducing the albumin-globulin ratio (0.45±0.02 vs 1.52± 0.15) in pyometra affected bitches. The pre-operative mean aspartate and alanine aminotransferase (AST, ALT) and alkaline phosphatase (ALP) activities were significantly higher (P<0.01) in bitches affected with pyometra (66.33 ± 9.84 and 73.78 ± 8.78 IU/l, and 86.78 ± 13.20 KAU %, respectively) as compared to healthy ones (32.50 ± 1.70, 31.25 ± 1.46 IU/l and 40.13 ± 2.59 KAU %). The levels of ALP were insignificantly higher in cases of closed pyometra than the open ones, but not of AST or ALT. The levels of all 3 enzymes dropped a little by 8-15 days following surgery in affected animals. Plasma progesterone profile was three times elevated (11.20 ± 2.88 vs 4.10 ± 0.91 ng/ml) and oestradiol-17b was insignificantly reduced (623.78 ± 235.75 vs 777.38 ±174.02 pg/ml) in pyometric bitches than the normal ones. Moreover, the mean progesterone concentration was significantly higher in cases of closed pyometra than the open ones (19.97 ± 2.88 vs 6.81 ± 2.59 ng/ml), and it declined significantly within 8-15 days following ovario-hysterectomy. It was concluded that significant elevation in serum biochemical and enzymatic profile together with progesterone in pyometric bitches as compared to normal ones with the drop in their levels post-operatively is of diagnostic and prognostic value in canine pyometra.
Inmunohistoquímica en Lesiones Cutáneas Melanociticas: Utllidad de los Marcadores Antígeno S100, HMB-45, Cromogranina A y Proteína p53

OBJECTIVES: To determine the clinicopathological features of patients with cutaneous melanoma, the role of Merckel cells in this tumour and diagnostic usefulness of celular markers. MATERIAL AND METHODS: Clinico-pathological correlation of 650 clinical records of patients with histopathological diagnosis of cutaneous melanoma was done. The Ag S100, HMB-45, cromogranin A, and p53 protein presence were assessed in 30 of these patients who also had pigmented benign skin lesions. RESULTS: Patients with melanoma had history of sunlight exposure and previous nevus or associated nevus. AgS100 was the more sensitive marker but had lesser specificity; HMB-45 was positive in lesions with malignant and active melanocytes whereas p53 was seen in certain melanoma cells. Proliferating Merckel cells were not found. CONCLUSIONS: S100 antigen must be used as diagnostic tool; HMB-45 had a lesser sensitivity. Melanomas, displasic nevus cells and sunlight dermatosis queratinocites were all p53-positive.
Przygotowanie pacjenta do rekontrukcji więzadła krzyżowego przedniego (ACL) – opis przypadku = Prepare the patient for the reconstruction of the anterior cruciate ligament (ACL) – case study

PRZYGOTOWANIE PACJENTA DO REKONTRUKCJI WIĘZADŁA KRZYŻOWEGO PRZEDNIEGO (ACL) – OPIS PRZYPADKU Prepare the patient for the reconstruction of the anterior cruciate ligament (ACL) – case study Bartosz Kochanski 1 , Krystian Kaluzny 1 , Monika Dylewska 2 , Marek Zielinski 3 , Walery Zukow 4 , Wojciech Hagner 1 1 Chair and Clinic of Rehabilitation of Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland 2 Bydgoszcz University, Bydgoszcz, Poland 3 Higher School of Humanities - Economics in Wloclawek, Poland 4 Kazimierz Wielki University in Bydgoszcz, Poland Streszczenie. Urazy stawu kolanowego stanowią okolo 70% wszystkich urazow w sporcie. Strukturą anatomiczną w obrebie stawu kolanowego, ktora najcześciej ulega uszkodzeniu jest wiezadlo krzyzowe przednie (ACL). Autorzy na przykladzie opisywanego przypadku przedstawili przygotowanie pacjenta do rekonstrukcji wiezadla krzyzowego przedniego. Opisano zalozenia oraz role rehabilitacji przedoperacyjnej. Zaprezentowano schemat postepowania diagnostycznego oraz program postepowania fizjoterapeutycznego przed rekonstrukcją ACL. Slowa kluczowe: ACL, rehabilitacja przedoperacyjna, rekonstrukcja Abstract. Knee injuries account for about 70% of all injuries in sports. Anterior cruciate ligament (ACL) is an anatomical structure which is most susceptible to damage in the knee. The authors present prepare the patient for anterior cruciate ligament reconstruction. Describes the assumptions and the role of preoperative rehabilitation. The paper presents a scheme of diagnostic and program of physiotherapy before ACL reconstruction. Key words: ACL, preoperative rehabilitation, reconstruction
Safety and immunogenicity of influenza A H5 subunit vaccines: effect of vaccine schedule and antigenic variant.

BACKGROUND The current US national stockpile of influenza H5 vaccine was produced using the antigen from the strain A/Vietnam/1203/2004 (a clade 1 H5 virus). Recent H5 disease has been caused by antigenically divergent H5 viruses, including A/Indonesia/05/2005 (a clade 2 H5 virus). METHODS The influence of schedule on the antibody response to 2 doses of H5 vaccines (one a clade 1 hemagglutinin protein [HA] vaccine and one a clade 2 HA vaccine) containing 90 μg of antigen was evaluated in healthy adults 18-49 years of age. RESULTS Two doses of vaccine were required to induce antibody titers ≥ 1:10 in most subjects. Accelerated schedules were immunogenic, and antibody developed after vaccinations on days 0 and 7, 0 and 14, and 0 and 28, with the day 0 and 7 schedule inducing lower titers than those induced with the other schedules. With mixed vaccine schedules of clade 1 followed by clade 2 vaccine administration, the first vaccination primed for a heterologous boost. The heterologous response was improved when the second vaccination was given 6 months after the first, compared with the response when the second vaccination was given after an interval of 1 month. CONCLUSIONS An accelerated vaccine schedule of injections administered at days 0 and 14 was as immunogenic as a vaccine schedule of injections at days 0 and 28, but both schedules were inferior to a vaccine schedule of injections administered at 0 and 6 months for priming for heterologous vaccine boosting. Clinical Trial Registry Number: NCT00703053.
Avaliaçao ultrassonografia da ecogenicidade do parênquima testicular como exame complementar no andrológico de carneiros

SUMMARY: The present study aimed to standardize an ultrasonogram method to testicular parenchyma in andrological sheep (n = 6). We made testicular biometric, spermiogram and testicular parenchyma ultrasonography, carrying images of the testicular parenchyma and measured the percentage of the echogenicity in gray scale on the device itself, correlating with the biometrics data and spermiogram. There were positive correlations between echogenicity and sperm concentration (p = 0.0192 and r2 = 0.4373) and between echogenicity and mass motility (p = 0.0358 and r2 = 0.37), demonstrating that the method is efficient to detect abnormal scrotal asymptomatic by conventional tests, contributing to animal breeding.
Aspects epidemiologiques-cliniques-radiologiques, therapeutiques et evolutifs du myelome multiple a l’hopital de l’amitie de BANGUI.

RESUME But: C ontribuer au diagnostic et a la prise en charge efficiente du myelome multiple a Bangui. Patients et Methode : Etude retrospective et descriptive concernant 15patients ayant des dossiers complets et de sexes confondus, retenus sur des criteres cliniques et para cliniques. Resultats : L’âge moyen etait de 66,8ans avec des extremes allant de 45-88ans et un sex-ratio de 6,5 en faveur des hommes. Les cultivateurs representaient 59,8% ; 66,6% des patients residaient en provinces et 33,4% a Bangui. Sur le plan clinique et para clinique, on notait 79,9% de manifestations osseuses, une alteration de l’etat generale(46,7%) et une pâleur cutaneo-muqueuse( 40%) ,une hyperleucocytose (superieure a 10000/mm 3 ) chez 40% de patients avec 53,8% de cas d’anemie et une thrombopenie ( 6,7%) ; une VS acceleree (93,3%) ainsi qu’ une plasmocytose medullaire chez tous les patients , suivi d’une hyper protidemie et une hypercalcemie dans 60%de cas chacune. Des lacunes osseuses a la radiographie (60%) et 66,7% de patients etaient au stade III de Salmon et Durie. Le traitement etait base sur VAD (vincristine, Adriamycine et Dexamethasone) pour une duree de 6mois (33,3%) et de 4mois (26,7%). Sur le plan evolutif, 60% des malades etaient perdus de vue, 26,7% de guerison et 13,3% de deces. Conclusion : La maladie de Kahler affectant les personnes âgees existe en realite en Centrafrique mais son diagnostic et sa prise en charge therapeutique sont tardifs dus a la meconnaissance de cette pathologie et de l’eloignement des malades. SUMMARY: Background: To contribute to the diagnosis and efficient management of multiple myeloma in Bangui. Patients and methods: It was a retrospective and descriptive study concerning 15 patients with complete records and sexes , selected on the basis of clinical and paraclinical data. Results: The average age was 66.8 year with extreme of 45-88ans and a sex ratio of 6.5 in favor of men. Farmers accounted for 59.8%, 66.6% of patients residing in provinces and 33.4% in Bangui. 79.9% presented with skeletal manifestations, 46.7% with poor general condition and 40% with mucocutaneous pallor; biologically, leukocytosis (greater than 10000/mm 3 ) was observed in 40% of patients with 53.8% of cases of anemia and thrombocytopenia (6.7%), accelerated ESR (93.3%) as well as a marrow plasmocytosis in all patients, followed by hyper serum protein and hypercalcemia in 60% of cases each. Bone defects in radiography were present in 60%, and 66.7% of patients were at stage III of Salmon and Durie. The treatment was based on VAD (vincristine, Adriamycin and Dexamethasone) for a period of 6 months (33.3%) patients and 4 months (26.7%). During follow-up, 60% of patients were reported lost, 26.7% were declared cured and 13.3% of death was observed. Conclusion: Kahler disease affecting the elderly is actually frequent in the Central Africa Republic, but its diagnosis and management are done late due to ignorance of the condition and the long distance of the patients from Bangui.
Descemet Membrane Endothelial Keratoplasty for Graft Failure After Descemet Stripping Endothelial Keratoplasty: Clinical Results and Histopathologic Findings.

IMPORTANCE The management of graft failure is increasingly relevant with the spread and growing acceptance of endothelial keratoplasty. OBJECTIVES To investigate the functional and anatomical results of secondary Descemet membrane endothelial keratoplasty (DMEK) for graft failure after Descemet stripping endothelial keratoplasty (DSEK) and to histologically analyze the stroma-to-stroma interface with respect to clinical implications. DESIGN, SETTING, AND PARTICIPANTS In a single-surgeon prospective comparative case series at the Department of Ophthalmology, Charité-University Medicine Berlin, Berlin, Germany, 8 eyes (3.8%) of 210 consecutively performed DMEK procedures underwent a secondary DMEK for graft failure after DSEK from March 1, 2012, through February 28, 2013. Those cases were compared with the eyes of a reference collective (n = 30) and matched-pairs group (n = 8) after primary DMEK for Fuchs endothelial dystrophy. INTERVENTION Descemet membrane endothelial keratoplasty. MAIN OUTCOMES AND MEASURES Postoperative best-corrected visual acuity (BCVA) and central corneal thickness at 1, 3, 6, and 12 months. Intraoperatively obtained DSEK graft lenticels were investigated immunohistochemically. RESULTS Patients with graft failure after DSEK had a mean (SD) age of 79.4 (7.2) years (range, 70-90 years). Preoperatively, the mean (SD) BCVA was 1.13 (0.50) logMAR (20/250 Snellen equivalents), and the mean (SD) central corneal thickness measured 704 (161) µm. Twelve months postoperatively, the mean (SD) corneal thickness decreased to 524 (27) µm after secondary and 516 (27) µm after primary DMEK (P = .57). A mean (SD) BCVA of 0.38 (0.36) logMAR (20/50 Snellen equivalents) was achieved after secondary DMEK compared with 0.15 (0.15) logMAR (20/28 Snellen equivalents) after primary DMEK. Histologically, failed DSEK graft lenticels presented condensations of collagen layers. Fibronectin and cytokeratin were accumulated along the stroma-to-stroma interface; vimentin was found in loosened graft stroma. CONCLUSIONS AND RELEVANCE These data suggest that DMEK might be considered a feasible choice in patients with graft failure after DSEK. However, the visual restitution might be impeded because of preceded depositions of matrix proteins within the corneal stroma and the stroma-to-stroma interface, which are associated with corneal fibrosis. Thereby, fibrotic processes might be avoided by performing a secondary DMEK in an early phase of graft failure.
Compliance with infant formula feeding by HIV-positive women one week after delivery in Khayelitsha, South Africa

When mixed feeding occurs a few days following delivery, the risk of HIV transmission is likely high. The study aim was to assess infant feeding practices, one week following delivery of HIV-positive mothers who intended to formula feed their infants. A consecutive sample of 95 HIV-positive mother-infant pairs was recruited soon after delivery from a midwife obstetric unit in Khayelitsha. Face-to-face interviews were conducted one week after delivery at the clinic to determine the actual infant feeding practices. Sixty-four HIV-positive mother-infant pairs completed the study. The response rate was 67%. The median interview day was day 8. Sixty-two mothers (97%) (95% CI: 95% to 99%) exclusively formula fed their infants. Fifty (78%) (95% CI: 73% to 83%) mothers gave their infants formula milk only. Two mothers breast-fed their babies. Twelve (19%) gave their babies other fluids or food. Eleven gave water, glucose water or gripe water and one gave cereal or porridge. Breast engorgement occurred in 51 (80%).
Radiological Assessment of Orbital Dimensions of the Kalabaris and Ikwerres of Rivers State, Nigeria

The use of Craniofacial measurements for ethno-racial and sex discrimination in forensic studies has been a major focus in recent times. This study compares orbital dimensions and index for the Ikwerre and Kalabaris tribes of Rivers State, Nigeria. 300 radiographs of the human skull, 150 females (both tribes) and 150 males (both tribes), were randomly selected from the Radiology Department in both Hospitals. The orbital length, width and depth were measured and orbital Index was calculated. The data were statistically analysed for means, standard deviation (SD) and Z-test (significant at p≤0.05). The Ikwerre male mean orbital length, width, depth and mean orbital index values were; 44.06±0.497, 42.87±0.531, 38.80±0.368 and 103.33±1.44 respectively, while Ikwerre female mean orbital length, width, depth and mean orbital index were; 44.26±0.448, 42.37±0.571, 39.08±0.377 and 105.25±1.24 respectively. The Kalabari male mean orbital length, width, depth and Index were; 44.67±0.402, 41.14±0.356, 38.27±0.337,103.98±0.956 respectively while Females mean orbital length, width, depth and Index were;42.22±0.441,41.14±0.380,38.89±0.401 and 102.92±1.10 respectively. No Sexual dimorphism (p>0.05) was observed for all orbital dimensions measured. However, there was a statistically significant difference in means of orbital length (between the two tribes in both sexes) and width (between tribes only in males). The result for the orbital index put both tribes into the megaseme category. The values for orbital dimensions of these indigenous tribes may be useful to clinicians and forensic anthropologist.
Role of DNA methylation in growth and differentiation in Physcomitrella patens and characterization of cytosine DNA methyltransferases

Epigenetic mechanisms such as DNA methylation are known to regulate important developmental processes in higher eukaryotes. However, little is known about the necessity and role of this process in early land plants. Using the methyltransferase (MTase) inhibitor zebularine (1‐(β‐d‐ribofuranosyl)‐1,2‐dihydropyrimidine‐2‐one), the impact of loss of genome‐wide methylation on the overall development in Physcomitrella patens was analyzed. It is observed that various aspects of growth and differentiation during gametophyte development become aberrant. A search for the core molecular components of methylation machinery, cytosine DNA MTases, revealed the presence of seven loci in the P. patens genome. Five of the loci code for MTases that are similar to corresponding proteins in higher plants, while two MTases appear specific to P. patens and are closely related to human DNMT3a and DNMT3b, respectively. These proteins possess all the conserved catalytic motifs characteristic of MTases and a domain of unknown function, DUF3444. Association of these highly conserved motifs with a DUF has not been reported in any of the MTases known so far. All the seven genes are differentially but ubiquitously expressed in gametophytes at low levels. Subcellular localization of GFP‐fused proteins shows patterns of distribution that can be correlated with their putative cellular functions. This work bridges the knowledge of MTases in P. patens and makes this simple model plant accessible for studies on epigenetic aspects that remain intractable in higher plants.