Miracle-dz/UniFAR-FTUs huggingface dataset:
A class of activation domains interacts directly with TFIIA and stimulates TFIIA-TFIID-promoter complex assembly

TFIIA is a general transcription factor that interacts with the TFIID-promoter complex required for transcription initiation by RNA polymerase II. Two lines of evidence suggest that TFIIA is directly involved in the mechanism by which some activators stimulate transcription. First, binding of TFIIA to a TFIID-promoter complex is a rate-limiting step that is enhanced by transcriptional activators GAL4-AH and Zta. Second, recombinant TFIIA greatly enhances activator-dependent transcription. In this study, we found that the activation domains of Zta and VP16 bind directly to TFIIA. Both Zta and VP16 stimulated rapid assembly of a stable TFIID-TFIIA complex on promoter DNA. Analysis of deletion derivatives of the VP16 activation domain indicated that the ability to bind to TFIIA correlates with the ability to enhance TFIID-TFIIA-promoter ternary complex assembly. Thus, we propose that a class of activators stimulate transcription initiation through direct interactions with both TFIIA and TFIID, which stimulate the assembly of an activated TFIIA-TFIID-promoter complex.
A Novel Plant Cysteine Protease Has a Dual Function as a Regulator of 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene Expressionw⃞

The hormone ethylene influences plant growth, development, and some defense responses. The fungal elicitor Ethylene-Inducing Xylanase (EIX) elicits ethylene biosynthesis in tomato (Lycopersicon esculentum) and tobacco (Nicotiana tabacum) leaves by induction of 1-aminocyclopropane-1-caboxylic acid synthase (Acs) gene expression. A minimal promoter element in the LeAcs2 gene required for EIX responsiveness was defined by deletion analysis in transgenic tomato plants. The sequence between −715 and −675 of the tomato Acs2 gene was found to be essential for induction by EIX. A Cys protease (LeCp) was isolated that specifically binds to this cis element in vitro. Ectopic expression of LeCp in tomato leaves induced the expression of Acs2. Moreover, chromatin immunoprecipitation showed that LeCp binds in vivo to the Acs promoter. We propose a mechanism for the dual function of the LeCp protein. The protease acts enzymatically in the cytoplasm. Then, upon signaling, a small ubiquitin-related modifier protein binds to it, enabling entrance into the nucleus, where it acts as a transcription factor. Thus, LeCp can be considered a dual-function protein, having enzymatic activity and, upon elicitor signaling, exhibiting transcriptional factor activity that induces LeAcs2 expression.
Physicians' motivation in the Ministry of Health and Population - Egypt: challenges and opportunities.

Amidst the different problems encountered at the Egyptian Ministry of Health (MOHP) and Population, the issue of physicians’ retention is on the rise. The Egyptian public health system reportedly lost more than five percent of its workforce of physicians in less than three years (20162018), as documented by CAPMAS and the Egyptian Medical Syndicate in 2016. Clinicians are not only skipping the practice from the MOHP, but even a number of academic institutions report a decreasing number of candidates interested in pursuing such a previously known attractive career path as faculty in the different schools of medicine. Figures about the increased migration rates of Egyptian doctors are also striking, partly attributable to the various hurdles they face within the MOHP. Adopting a qualitative research approach, the motives of clinicians to practice in the public sector are investigated in this study. Theories and definitions of motivation are explored to explain how motivation starts and what is required to maintain it. The research showed that various factors push and pull Egyptian doctors from practice in the MOHP; their individual motivations highly change due to organizational and cultural conditions. Reported constraints included the challenging career development opportunities, the inadequate infrastructure, as well as inefficient management, and inadequate legislative environment. Doctors’ attrition, shifting to private practice and migration to the Arab and Gulf countries are some commonly encountered consequences of low motivation. Physicians’ shortage is an issue in both developing and developed countries. Securing the needed human resources for the health care services is vital. Several policies were developed to bridge this gap, including performance-based financing and training complementary personnel. In Egypt, some measures were adopted such as obliging fresh graduates to fill in the gaps and piloting the delegation of certain tasks to mid-level personnel. However, as the current study indicates, additional expenditure on health is the real step that the Egyptian health system should implement to ensure healthier living conditions for the most underprivileged citizens. Reforms in governance and administration should follow, with changes to medical education and training entities.
Current state of knowledge on Takotsubo syndrome: a Position Statement from the Taskforce on Takotsubo Syndrome of the Heart Failure Association of the European Society of Cardiology

Takotsubo syndrome is an acute reversible heart failure syndrome that is increasingly recognized in modern cardiology practice. This Position Statement from the European Society of Cardiology Heart Failure Association provides a comprehensive review of the various clinical and pathophysiological facets of Takotsubo syndrome, including nomenclature, definition, and diagnosis, primary and secondary clinical subtypes, anatomical variants, triggers, epidemiology, pathophysiology, clinical presentation, complications, prognosis, clinical investigations, and treatment approaches. Novel structured approaches to diagnosis, risk stratification, and management are presented, with new algorithms to aid decision‐making by practising clinicians. These also cover more complex areas (e.g. uncertain diagnosis and delayed presentation) and the management of complex cases with ongoing symptoms after recovery, recurrent episodes, or spontaneous presentation. The unmet needs and future directions for research in this syndrome are also discussed.
Chitosan-Hydroxypropyl Methylcellulose Matrices as Carriers for Hydrodynamically Balanced Capsules of Moxifloxacin HCl.

BACKGROUND In recent years, gastroretentive, hydrodynamically balanced system (HBS) for stomach-specific floating sustained drug release has gained a lot of importance in improving absorption of drugs especially those absorbed from stomach and small intestine. OBJECTIVE The objective of the current investigation is to evaluate chitosan-hydroxypropyl methylcellulose (HPMC) based on polymeric matrices as a carrier for single-unit capsules based on HBS for stomach- specific floating sustained drug release using moxifloxacin HCl (MX) as a model drug. METHOD Various HBS capsules of MX were prepared by physical blending of MX with chitosan (low or medium molecular mass) or HPMC (K4M or K15M) or chitosan-HPMC combinations in varying proportions followed by encapsulation into size 0 capsules made of hard gelatin. The in vitro buoyancy and drug release in 0.1 N HCl (pH 1.2) were evaluated. RESULTS HBS capsules based on chitosan (low and medium molecular weight and their combination) as polymer matrix failed to float on 0.1 N HCl (pH 1.2). Whereas, formulations containing HPMC (K4M or K15M) or their mixture with chitosan, remained buoyant and released MX over 9 h in the acidic dissolution medium following zero-order kinetics. CONCLUSION HPMC (K4M, K15M, blend of K4M and K15M) or their mixture with low/medium molecular mass chitosan may constitute excellent carrier systems for the stomach-specific sustained delivery of MX over a longer period.
HLA-DQ-Restricted, Islet-Specific T-Cell Clones of a Type I Diabetic Patient: T-Cell Receptor Sequence Similarities to Insulitis-Inducing T-Cells of Nonobese Diabetic Mice

We established a T-cell line and 20 CD4+ T-cell clones from the peripheral blood lymphocytes of a type I diabetic patient using a membrane preparation of a rat insulinoma cell line (beta membrane antigen [BMA]) as a source of antigen. The T-cell line and three selected clones proliferated specifically to stimulation with BMA and to membranes prepared from human islets, rat pancreas, and NOD pancreas, but not to control antigens. Proliferationinhibition studies using human leukocyte antigen (HLA)-specific monoclonal antibodies revealed HLA-DQw1-restricted recognition of BMA. An analysis of the T-cell receptor (TCR) repertoire of the T-cell line after 8 and 40 days of culture showed a prominent usage of the Val and Vα12 gene families. Although sequencing of the TCR Vα and Vβ chains of the three clones demonstrated that each used different Va and V(J gene segments, structural similarities were found in complementary-determining region 3 (CDR3), the region that is postulated to interact with the peptide component of the TCR ligand. When we compared these TCR sequences with published sequences of disease-inducing T-cells of NOD mice, highly related TCR Vβ families were detected. Furthermore, stretches of identical amino acids within the CDR3 region were found between two pairs of human and mouse T-cells. If one considers the species differences in TCR genes and sequence differences in the restriction elements for these cells (HLA-DQ vs. H-2I-Anod), these sequence similarities are striking and may be useful for pinpointing T-cells of primary importance in the development of disease.
The influence of age at migration and length of residence on self-rated health among Swedish immigrants: a cross-sectional study

Objective. Increasing global migration has led to profound demographic changes in most industrialised countries. A growing body of research has investigated various health aspects among immigrant groups and found that some immigrant groups have poorer health than the majority population. It has been suggested that poor acculturation in the host country could lie behind the increased risk of worsened health among certain immigrant groups. The aim was to investigate the cross-sectional association between acculturation, measured as age at migration or length of residence, and self-rated health among young immigrants. Design. The simple, random samples of 7137 women and 7415 men aged 16–34 years were based on pooled, independent data collected during the period 1992–1999 obtained from the Swedish Annual Level of Living Survey (SALLS). Logistic regression was applied in the estimation of odds ratios (OR) for poor self-rated health, after accounting for age, sex, socioeconomic status (SES) and social networks. The non-response rate varied between 23.6 and 28.3% in the different immigrant groups. Results. The odds of poor self-rated health increased with increasing age at migration to Sweden among first-generation immigrants. For those who had resided in Sweden less than 15 years the odds of poor self-rated health were significantly increased. In addition, most of the immigrant groups had higher odds of poor self-rated health than the reference group. Conclusions. Health care workers and policy makers need to be aware that immigrants who arrive in the host country at higher ages and/or have lived in the host country for a shorter period of time might need special attention as they are more likely to suffer from poor self-rated health, a valid health status indicator that can be used in population health monitoring.
Comparative histochemical investigation of the glutathione S-transferase placental form and gamma-glutamyltranspeptidase during N-nitrosobis(2-hydroxypropyl)amine-induced lung carcinogenesis in rats.

Immunohistochemical staining using anti-rat glutathione S-transferase placental form (GST-P) rabbit antibody and enzyme histochemical staining for gamma-glutamyltranspeptidase (gamma-GT) were investigated in lesions appearing during lung carcinogenesis induced by N-nitrosobis(2-hydroxypropyl)amine (BHP) in rats. Rats were given BHP at a concentration of 2000 p.p.m. in drinking water, and were killed after 12 weeks of BHP intake, after 12 weeks of BHP intake followed by 12 weeks of tap water intake or after 20 weeks of continuous BHP intake. It was found that bronchiolo-alveolar hyperplasias, adenomas, adenocarcinomas, squamous metaplasias and squamous cell carcinomas had been induced by BHP. All of the squamous metaplasias and squamous cell carcinomas were shown to stain with GST-P but not with gamma-GT. On the other hand, the hyperplasias, adenomas and adeno-carcinomas stained with gamma-GT to various degrees and in different areas, but did not stain with GST-P. The incidence of gamma-GT phenotype and the average percentage of gamma-GT-positive areas in hyperplasias and adenomas suggested that adenocarcinomas might develop from hyperplasias and adenomas. These results suggest that GST-P is a marker for squamous lesions while gamma-GT is a marker for adenomatous lesions in rat lung carcinogenesis. Furthermore, squamous metaplasias appear to be preneoplastic lesions of squamous cell carcinomas while gamma-GT-positive hyperplasias or adenomas are preneoplastic lesions of peripheral adenocarcinomas.
Preparing and Developing Leaders for Indigenous-Serving Schools via the Holistic Blessing of POLLEN's Leadership Tree

Abstract:Faculty in a flagship university in the U.S. Southwest collaborated with Indigenous stakeholders to design and implement an educational leadership program for aspiring leaders of Indigenous-serving schools. This study explores the perceptions of participants in that leadership program. The study utilizes data collected through an external program evaluation that employed focus group interviews with graduate students and interviews with faculty. Data were collected and analyzed in a process of coding and generating themes. Results indicate that the educational leadership program for Indigenous students was most useful and relevant when centered on social/relational content, linked to spiritual/cultural roots, in harmony with Indigenous wisdom, and grounded in place and well-being. The lessons learned sustained the project through enrollment of three cohorts and may have broader implications for funders who wish to support leadership programs for longer-term influence, as well as universities who aim to make a greater investment in their Indigenous communities.
8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity.

Substrate specificities of FPG protein (also known as formamidopyrimidine DNA glycosylase) and 8-hydroxyguanine endonuclease were compared by using defined duplex oligodeoxynucleotides containing single residues of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydro-2'-deoxyadenosine (8-oxodA), and 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine (Me-Fapy). Duplexes containing 8-oxodG positioned opposite dC, dG, or dT were cleaved, whereas single-stranded DNA and duplexes containing 8-oxodG.dA or 8-oxodA positioned opposite any of the four DNA bases were relatively resistant. Both enzymes cut duplexes containing 8-oxoG.dC 3' and 5' to the modified base but failed to cleave duplex DNA containing synthetic abasic sites, mismatches containing dG, or unmodified DNA. 8-Oxoguanine, identified by HPLC-electrochemical detection techniques, was released during the enzymatic reaction. Apparent Km values for FPG protein acting on duplex substrates containing a single Me-Fapy or 8-oxodG residue positioned opposite dC were 41 and 8 nM, respectively, and those for 8-hydroxyguanine endonuclease were 30 and 13 nM, respectively. Comparison of the properties of the two enzyme activities suggest that they are identical. In view of the widespread distribution of 8-oxodG in cellular DNA, the demonstrated miscoding and mutagenic properties of this lesion, and the existence of a bacterial gene coding for FPG protein, we propose that 8-oxodG DNA is the primary physiological substrate for a constituent glycosylase found in bacteria and mammalian cells.
Do professional interpreters improve clinical care for patients with limited English proficiency? A systematic review of the literature.

OBJECTIVE To determine if professional medical interpreters have a positive impact on clinical care for limited English proficiency (LEP) patients. DATA SOURCES A systematic literature search, limited to the English language, in PubMed and PsycINFO for publications between 1966 and September 2005, and a search of the Cochrane Library. STUDY DESIGN Any peer-reviewed article which compared at least two language groups, and contained data about professional medical interpreters and addressed communication (errors and comprehension), utilization, clinical outcomes, or satisfaction were included. Of 3,698 references, 28 were found by multiple reviewers to meet inclusion criteria and, of these, 21 assessed professional interpreters separately from ad hoc interpreters. Data were abstracted from each article by two reviewers. Data were collected on the study design, size, comparison groups, analytic technique, interpreter training, and method of determining the participants' need for an interpreter. Each study was evaluated for the effect of interpreter use on four clinical topics that were most likely to either impact or reflect disparities in health and health care. PRINCIPAL FINDINGS In all four areas examined, use of professional interpreters is associated with improved clinical care more than is use of ad hoc interpreters, and professional interpreters appear to raise the quality of clinical care for LEP patients to approach or equal that for patients without language barriers. CONCLUSIONS Published studies report positive benefits of professional interpreters on communication (errors and comprehension), utilization, clinical outcomes and satisfaction with care.
Analysis and design of a high-performance planar Marchand balun

This paper presents an enhanced Marchand balun that offers excellent amplitude and phase balance performance. The enhanced Marchand balun is designed using compensated coupled lines. It employs capacitive compensation, a renowned technique for compensating the unequal even- and odd-mode phase velocities encountered in parallel-coupled microstrips. Analysis carried out in this study has proven that the finite directivity of coupled lines significantly affects the balun performance. The proposed capacitively-compensated Marchand balun is demonstrated at 2.1 GHz and has offered excellent results.
Glatiramer acetate: long-term safety and efficacy in relapsing-remitting multiple sclerosis

Glatiramer acetate (GA) is approved for relapsing-remitting multiple sclerosis in 57 countries worldwide, with more than 2 million patient-years of exposure and over 20 years of continuous clinical use without new safety concerns. GA has an overall favorable risk–benefit profile: 30% reduced annual relapse rate and decreased brain lesion activity. In clinically definite MS or clinically isolated syndrome, GA slows brain atrophy, which may be related to its unique anti-inflammatory and neuroprotective mechanisms of action. Early treatment with GA delays the onset of clinically definite MS more effectively than late treatment in clinically isolated syndrome. GA is not associated with immunosuppression, autoimmune disease, infections or development of neutralizing antibodies. A new three-times-weekly formulation of GA is available to potentially reduce the incidence of injection-related side effects. Other safety advantages of GA include its pregnancy rating (Category B) and limited uncontrolled data suggesting that tolerability is similar in children with MS.
Identification of Genes in Thuja plicata Foliar Terpenoid Defenses1[C][W][OA]

Transcriptome profiling of foliage with and without resin glands identifies candidate gene products in thujone biosynthesis. Thuja plicata (western redcedar) is a long-lived conifer species whose foliage is rarely affected by disease or insect pests, but can be severely damaged by ungulate browsing. Deterrence to browsing correlates with high foliar levels of terpenoids, in particular the monoterpenoid α-thujone. Here, we set out to identify genes whose products may be involved in the production of α-thujone and other terpenoids in this species. First, we generated a foliar transcriptome database from which to draw candidate genes. Second, we mapped the storage of thujones and other terpenoids to foliar glands. Third, we used global expression profiling to identify more than 600 genes that are expressed at high levels in foliage with glands, but can either not be detected or are expressed at low levels in a natural variant lacking foliar glands. Fourth, we used in situ RNA hybridization to map the expression of a putative monoterpene synthase to the epithelium of glands and used enzyme assays with recombinant protein of the same gene to show that it produces sabinene, the monoterpene precursor of α-thujone. Finally, we identified candidate genes with predicted enzymatic functions for the conversion of sabinene to α-thujone. Taken together, this approach generated both general resources and detailed functional characterization in the identification of genes of foliar terpenoid biosynthesis in T. plicata.
How Do Trade and Domestic Policy Variables Stand among Influencers of Grain Price Volatility in Nigeria

Food price volatility has become an issue of great concerns to researchers, policy makers, governments and development workers worldwide, Nigeria inclusive. Against the background of limited, and mixed findings on the nature, and drivers of price volatility in Nigeria (and some other African countries), developing effective policies to calm food price instability remains a speculation. Consequently, this study examined the potential roles of trade and domestic policies, including certain external and local factors on price volatility of specific grains in Nigeria. Time time series data from different secondary sources were used for analysis with a dynamic panel data (fixed effects) model as the main econometrics model. Except for domestic price of imported rice, price volatility in grain markets have not increased markedly in the wake of global food crises. Whereas tariffs and trade openness play insignificant role in explaining volatility in grain markets, political stability, narrow money supply, petrol price, interest and exchange rates, and volatile crude oil price, exerts significant influence, howbeit differently, on price volatility of specific grains. While challenging the idea of volatility increase in general food prices, and the notion that international price upsurge is the dominant drivers of food price volatility in Africa, the findings call for greater attention on domestic factors such as political stability, management of monetary policy, including exchange rates as well as definitive actions to ensure stable petrol price as important pathways to address short and medium-term food price volatility in Nigeria.