Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of Hepatitis C Virus Core Protein Multimerization and Membrane Envelopment: Revelation of a Cascade of Core-Membrane Interactions

ABSTRACT The molecular basis underlying hepatitis C virus (HCV) core protein maturation and morphogenesis remains elusive. We characterized the concerted events associated with core protein multimerization and interaction with membranes. Analyses of core proteins expressed from a subgenomic system showed that the signal sequence located between the core and envelope glycoprotein E1 is critical for core association with endoplasmic reticula (ER)/late endosomes and the core's envelopment by membranes, which was judged by the core's acquisition of resistance to proteinase K digestion. Despite exerting an inhibitory effect on the core's association with membranes, (Z-LL)2-ketone, a specific inhibitor of signal peptide peptidase (SPP), did not affect core multimeric complex formation, suggesting that oligomeric core complex formation proceeds prior to or upon core attachment to membranes. Protease-resistant core complexes that contained both innate and processed proteins were detected in the presence of (Z-LL)2-ketone, implying that core envelopment occurs after intramembrane cleavage. Mutations of the core that prevent signal peptide cleavage or coexpression with an SPP loss-of-function D219A mutant decreased the core's envelopment, demonstrating that SPP-mediated cleavage is required for core envelopment. Analyses of core mutants with a deletion in domain I revealed that this domain contains sequences crucial for core envelopment. The core proteins expressed by infectious JFH1 and Jc1 RNAs in Huh7 cells also assembled into a multimeric complex, associated with ER/late-endosomal membranes, and were enveloped by membranes. Treatment with (Z-LL)2-ketone or coexpression with D219A mutant SPP interfered with both core envelopment and infectious HCV production, indicating a critical role of core envelopment in HCV morphogenesis. The results provide mechanistic insights into the sequential and coordinated processes during the association of the HCV core protein with membranes in the early phase of virus maturation and morphogenesis.
Analyst following and pay-performance sensitivity: evidence from China

ABSTRACT We examine whether analyst following is associated with pay-performance sensitivity in China. Based on our analyses of 17,020 Chinese firm-years from 2007 to 2015, we find that pay-performance sensitivity is higher when companies are followed by financial analysts. We also find that the analyst following/pay-performance sensitivity relationship is stronger for non-state-owned enterprises (SOEs) than for SOEs. Overall, our results indicate that companies followed by financial analysts exhibit more pay-performance-sensitivity, which is consistent the notion that financial analysts can serve an external monitoring role.
Lack of intracellular replication of M. tuberculosis and M. bovis BCG caused by delivering bacilli to lysosomes in murine brain microvascular endothelial cells

Invasion and traversal of the blood-brain barrier (BBB) by Mycobacterium tuberculosis cause meningeal tuberculosis (TB) in the central nervous system (CNS). Meningeal TB is a serious, often fatal disease that disproportionately affects young children. The mechanisms involved in CNS invasion by M. tuberculosis bacilli are poorly understood. In this study, we microscopically examined endosomal trafficking and measured survival of M. tuberculosis and M. bovis Bacille Calmette-Guérin (BCG) bacilli in murine brain microvascular endothelial cells (BMECs). The results show that both species internalize but do not replicate in BMECs in the absence of a cytotoxic response. Confocal microscopy indicates that bacilli-containing vacuoles are associated with the early endosomal marker, Rab5, late endosomal marker, Rab7, and lysosomal marker, LAMP2, suggesting that bacilli-containing endosomes mature into endolysosomes in BMECs. Our data also show that a subset of intracellular M. tuberculosis, but not BCG bacilli, escape into the cytoplasm to avoid rapid lysosomal killing. However, the intracellular mycobacteria examined cannot spread cell-to-cell in BMECs. Taken together, these data show that with the exception of the small terminal cytoplasmic population of bacilli, M. tuberculosis does not modulate intracellular trafficking in BMECs as occurs in macrophages and lung epithelial and endothelial cells.
Endogenous MCP-1 influences systemic cytokine balance in a murine model of acute septic peritonitis.

Sepsis and septic syndrome represent an intense systemic response with multiple physiologic and immunologic abnormalities, leading to multiple organ failure. Recent investigations suggest that the critical conditions are balanced by endogenous cytokines. In the present study, we examined the involvement of endogenous monocyte chemoattractant protein (MCP)-1 in the regulation of cytokine production in tissue/organs in a murine model of acute septic peritonitis induced by cecal ligation and puncture (CLP). Initial studies showed that CLP induced elevated levels of MCP-1 in tissues, such as liver, lung, and kidney. To neutralize endogenous MCP-1, either anti-MCP-1 antibodies or control antibodies were intraperitoneally administered 2 h prior to CLP. Administration of anti-MCP-1 antibodies resulted in a decrease in the level of interleukin (IL)-13 in tissues, while increasing the level of tumor necrosis factor-alpha, compared to control. In addition, anti-MCP-1 treatment decreased the level of IL-12 and, in contrast, increased the level of IL-10 in specific tissues. These findings suggest that endogenous MCP-1 influences the cytokine balance in tissues in favor of anti-inflammatory and immune-enhancing cytokines, probably protecting the host from tissue/organ damage during sepsis.
Performance Optimization for Cooperative Multiuser Cognitive Radio Networks with RF Energy Harvesting Capability

We study the performance optimization problem for a cognitive radio network with radio frequency (RF) energy harvesting capability for secondary users. In such networks, the secondary users are able to not only transmit packets on a channel licensed to a primary user when the channel is idle, but also harvest RF energy from the primary users' transmissions when the channel is busy. Specifically, we propose a system model where the secondary users are able to cooperate to maximize the overall network throughput through sensing a set of common channels. We first consider the case where the secondary users cooperate in a TDMA fashion and propose a novel solution based on a learning algorithm to find optimal channel access policies for the secondary users. Then, we examine the case where the secondary users cooperate in a decentralized manner and we formulate the cooperative decentralized optimization problem as a decentralized partially observable Markov decision process (DEC-POMDP). To solve the cooperative decentralized stochastic optimization problem, we apply a decentralized learning algorithm based on the policy gradient and the Lagrange multiplier method to obtain optimal channel access policies. Extensive performance evaluation is conducted and it shows the efficiency and the convergence of the learning algorithms.
The embodied and the cultural in the conceptualization of pitch space in
Croatian

Most conceptual metaphors that conceptualize musical pitch rely heavily on human perception, images and experience structured through spatial and orientation image schemas such as the schema of VERTICALITY. The paper analyses conceptual metaphors that structure pitch relations in terms of vertical space, thickness and size as they appear in the Croatian musical terminology. The image schemas of VERTICALITY and SIZE are analysed within the conceptual metaphors PITCH RELATIONS ARE RELATIONS IN VERTICAL SPACE and PITCH RELATIONS ARE RELATIONS IN SIZE in order to define to what extent their motivation is embodied and universal, and what can be attributed to cross-cultural and cross-linguistic influences present in the creation and understanding of music terminology in Croatia.
How Does Successful Off-Pump Pulmonary Vein Isolation for Paroxysmal Atrial Fibrillation Influence Heart Rate Variability and Autonomic Activity?

Objective Surgical pulmonary vein isolation (PVI) for paroxysmal atrial fibrillation (PAF) blocks trigger stimulation from PVs and partially disconnects the atria from sympathetic and parasympathetic neural stimulation. This study describes long-term changes in heart rate variability (HRV) and autonomic activity (AA) after successful bipolar radiofrequency PVI. Methods Twenty-seven patients who underwent coronary artery bypass grafting and successful (defined as stable sinus rhythm for 1 year) off-pump bipolar radiofrequency PVI for PAF were prospectively followed 3, 6, and 12 months after surgery including 24 hours Holter electrocardiogram. The following HRV and AA parameters were calculated: mean NN-interval, SD of NN-intervals, SD of averaged NN-intervals, root mean square of successive differences, low frequency (LF) power (0.04–0.15 Hz; a parameter specific for sympathetic activity), high frequency (HF) power (0.15–0.4 Hz; a parameter specific for parasympathetic activity), and the LF:HF ratio. Results Preoperatively, high HRV and AA parameters were recorded. In 3-, 6-, and 12-month time, a progressive reduction of HRV and AA was observed, reaching significance after 12 months. Respective rates before surgery and 12 months after it were: for SD of averaged NN-intervals (122.4 ± 113; 80.5 ± 42 milliseconds; P = 0.046), for root mean square of successive differences (79.2 ± 93; 45 ± 20 milliseconds; P = 0.04). The LF:HF ratios were 1.22 and 0.73 before and 12 months after surgery, respectively. The statistically significant continuous reduction in LF:HF ratio (P = 0.02) is suggestive of a progressive parasympathetic dominance 12 months after surgery. Conclusions Successful PVI for PAF results in HRV and sympathetic activity reduction with preoperative sympathetic dominance and oncoming vagal dominance after 1 year from surgery. Despite preoperative sympathetic dominance, successful PVI for PAF results in HRV and a reduction in sympathetic activity with emerging parasympathetic dominance 12 months after surgery.
Quality of Corporate Social Responsibility Disclosure and Cost of Equity Capital: Lessons from China

ABSTRACT This article explores the relationship between the quality of corporate social responsibility (CSR) disclosure and the cost of equity capital by analyzing the financial data and CSR reports of A-share listed firms in China from 2008 to 2014. The quality of the CSR disclosure is shown to be negatively related to the cost of equity capital of the listed firms. This negative correlation proves to be more prominent among firms of environmentally sensitive industries. Taking the ownership of the listed firms into consideration, it is further confirmed that the negative relationship between the CSR disclosure and the cost of equity capital is of higher significance for state-owned enterprises. Our findings also empirically demonstrate that the quality of CSR disclosure is more negatively related to the cost of equity capital among the large listed firms than the smaller ones.
The Waning Impact of School Finance Litigation on Inequality in Per Student Revenue during the Adequacy Era

Since 1989, most states have faced “adequacy” lawsuits that target state constitutions’ education clauses in aiming to boost funding provided to disadvantaged districts – which should reduce overall inequality in school funding. Using pooled time-series data on 44 states over 19 years spanning the adequacy era, this study examines how adequacy lawsuits affect inequality in total per-student revenue within states. Three main findings emerge from the analyses. First, across all states on average, inequality in revenue declined from 1990 to 2000 but then rose steadily from 2001 onward. Second, revenue inequality was substantially lower in years following the filing of an adequacy lawsuit compared to years preceding the lawsuit. Third, the inequality-reducing impact of adequacy litigation was limited to the years 1990–2000; litigation had no significant impact on revenue inequality during the period 2001–2011. I conclude that 1990–2000 was a much more active and impactful period compared to 2001–2011 in terms of adequacy litigation, raising questions about the future of school finance litigation.
Consumer Preferences and Welfare Evaluation under Current Food Inspection Measures in China: Evidence from Real Experiment Choice of Rice Labels

Frequent food quality and safety issues result in various food inspection measures in China, while some are not widely acknowledged by the public and are less efficient. Consumer demand is significant for priority setting in food policy. This study investigates Chinese consumers’ heterogeneous preferences for selected food inspection measures and estimates welfare effects based on willingness-to-pay (WTP) calculation. Rice consumption data from a 2018 nationwide consumer survey designed using the real choice experiment is analyzed by the random parameters logit and the latent class model. The findings reveal that consumers place a high value on government certification, and brand is valuable especially when public management is perceived as weak. However, the insufficient market demand for third-party certification may increase transaction costs due to overlapping functions and consumers’ distrust. Moreover, there should be a need to broaden consumers’ understanding of traceability and grading systems. This study emphasizes the necessity of direct governmental involvement and the existence of unnecessary policy cost.
Prevalence and genotype distribution of human papillomavirus among females in the suburb of Shanghai, China

To describe the prevalence of human papillomavirus (HPV) and its genotype distribution among females in the suburb of Shanghai. A total of 33 562 participants were enrolled in this study from January to December 2016. HPV GenoArray test kit was used to perform HPV genotyping and was also used in DNA amplification and HybriBio's proprietary flow‐through hybridization technique. The overall prevalence of HPV was 18.98% and the top ten genotypes of HPV infection were HPV 16 (3.36%), HPV 58 (2.65%), HPV 52 (2.48%), HPV 51 (1.58%), HPV 54 (1.40%), HPV 68 (1.32%), HPV 18 (1.23%), HPV 6 (1.15%), HPV 56 (1.10%), and HPV 33 (1.07%). Single infection (4749, 14.15%) was the most common types among all the infected cases. Significant differences were found among age groups and month groups in terms of simple and multiple infection (P < 0.05), pure HR, LR and mixed HPV infection (P < 0.05). The prevalence of HR and LR HPV infection among females in the suburb of Shanghai is high, prevalence of single and multiple infection, pure HR, LR and mixed infection is correlated with the age and month.
Policy Brief—Recommendations for Improving the Treatment of Risk and Uncertainty in Economic Estimates of Climate Impacts in the Sixth Intergovernmental Panel on Climate Change Assessment Report

Large discrepancies persist between projections of the physical impacts of climate change and economic damage estimates. These discrepancies increase with increasing global average temperature projections. Based on this observation, we recommend that in its Sixth Assessment Report (AR6), the Intergovernmental Panel on Climate Change (IPCC) improve its approach to the management of the uncertainties inherent in climate policy decisions. In particular, we suggest that the IPCC (1) strengthen its focus on applications of decision making under risk, uncertainty, and outright ambiguity and (2) estimate how the uncertainty itself affects its economic and financial cost estimates of climate damage and, ultimately, the optimal price for each ton of carbon dioxide released. Our hope is that by adopting these recommendations, AR6 will be able to resolve some of the documented inconsistencies in estimates of the physical and economic impacts of climate change and more effectively fulfill the IPCC’s mission to provide policymakers with a robust and rigorous approach for assessing the potential future risks of climate change.
Longitudinal analysis of bone density in human immunodeficiency virus-infected women.

OBJECTIVES The objective of the study was to investigate change in bone mineral density (BMD) over time in HIV-infected women in comparison with healthy control subjects similar in age, race, and body mass index (BMI). DESIGN This was a prospective cohort study. METHODS BMD was measured by dual-energy x-ray absorptiometry in 100 HIV-infected females and 100 healthy controls similar in age (41 +/- 1 vs. 41 +/- 1 yr, P = 0.57), BMI (26.1 +/- 0.5 vs. 27.2 +/- 0.4 kg/m(2), P = 0.12), and race (60 vs. 65% non-Caucasian, P = 0.47, HIV-infected vs. controls). Changes in BMD were determined every 6 months over 24 months. RESULTS At baseline, HIV-infected subjects had lower BMD at the lumbar spine (1.01 +/- 0.01 vs. 1.07 +/- 0.01 g/cm(2), P = 0.001), hip (0.94 +/- 0.01 vs. 0.98 +/- 0.01 g/cm(2), P = 0.02), and femoral neck (0.83 +/- 0.01 vs. 0.87 +/- 0.01 g/cm(2), P = 0.02). Historical low weight, duration of nucleoside reverse transcriptase inhibitor use, and FSH were significantly associated with lumbar BMD, whereas duration of HIV, BMI, historical low weight, smoking pack-years, N-telopeptide of type 1 collagen, viral load, 25 hydroxyvitamin D, and osteocalcin were associated with hip BMD at baseline. In mixed model longitudinal analyses, BMD remained lower in HIV-infected subjects than in controls over 24 months of follow-up (P = 0.001 for the spine, P = 0.04 for the hip, and P = 0.02 for the femoral neck). These differences remained significant controlling for age, race, BMI, and menstrual function. In contrast, rates of change for the spine (P = 0.79), hip (P = 0.44), and femoral neck (P = 0.34) were not different between the HIV and control groups over 2 yr. In the HIV group, longitudinal changes in BMD were not associated with current protease inhibitor, nucleoside reverse transcriptase inhibitor, or non-nucleoside reverse transcriptase inhibitor use but were associated with CD4 count, weight, FSH, N-telopeptide of type 1 collagen, and baseline BMD. CONCLUSIONS BMD is reduced at the spine, hip, and femoral neck among women with HIV in relationship to low weight, duration of HIV, smoking, and increased bone turnover. Over 2 yr of follow-up, BMD remained stable but lower in HIV-infected women, compared with control subjects.
A study of pupil participation in learning : factors which promote or undermine inclusion

This small-scale piece of practitioner research centres on the participation and learning experience of one group of year 7/8 pupils. My aim was to increase pupil participation in learning. I considered the pupil experience of inclusion and factors which promoted or undermined participation in learning. The investigation is in two phases. The first stage is a case study in which I investigate the school's organisational culture and how the features of school impinge on pupils' participation and inclusion. The second phase is classroom-based research, which is divided into four action cycles, each progressively focusing on an improvement in pupil participation in learning,which emerged from the previous cycle. Findings showed that the biggest barriers to pupils' learning were the school's organisational culture (which led to pupils' low self-esteem), over reliance on teacher support and lack of autonomy. Pupils' participation improved, together with their self-esteem by being given a choice and a voice, by being supported in identifying their learning styles and by teachers having high expectations of them in completing tasks which made sense and had purpose.
Plasma exchange in endocrine ophthalmopathy

We studied the effects of intensive plasma exchange on endocrine ophthalmopathy in 12 patients with Graves' disease and one with Hashimoto's thyroiditis. All patients were euthyroid at the time of plasma exchange. All but five had concomitant treatment with azathioprine. Each patient had a treatment period consisting of six plasma exchanges performed in 2–3 weeks; two patients were treated in two periods. Each time a mean of 2.4 liters plasma was exchanged.
Foreign body in the bronchus in children: 22 years experience in a tertiary care paediatric centre

Background: Our objective was to assess types, presentation, duration of symptoms and usefulness of rigid bronchoscopy for diagnosis and treatment of bronchial foreign body (FB) in children. Materials and Methods: Records of children with documented FB aspiration treated in Department of Paediatric Surgery from January 1991 to December 2012 were analysed retrospectively. Diagnosis was made on the basis of history, clinical examination, radiological evaluation and bronchoscopy. Results: A total of 196 children underwent emergency rigid bronchoscopy for suspected bronchial FB and in 173 cases FB was found. Out of 173 cases, 118 (68.21%) were males and 55 (31.79%) were females. Mean age was 3.7 years (range: 2 months-12 years) while mean duration of symptoms was 28 h (range: from 3 h to 4 months). Most common FB bronchus found was peanut 141 (81.50%). FB was localised to right bronchus in 112 (64.74%) cases while in 44 (25.43%) cases left bronchus was involved. In 17 (9.83%) cases FB was seen at carina only. Cough was the most common presenting symptom in 131 (75.72%) cases. The most common finding in chest X-ray was consolidation-collapse lung or emphysematous lung in 83 (47.97%) cases followed by the flattening of the diaphragm in 17 (9.83%) cases. In 35 (20.23%) cases chest X-ray was found to be normal. Pre-operative endotracheal intubation was done in 13 (7.51%) cases while 20 (11.56%) cases required post-operative mechanical ventilation. Conclusion: High index of suspicion should be kept for bronchial FB in children who present with suggestive history of FB ingestion even with normal physical and radiological evaluation.
Communication skills shape voice effects in organizations

ABSTRACT Benefiting from constructive communication theory, in this study, we contend that voice behavior will interact with the employee's functional communication competence or communication skills to predict workplace behaviors and motivation. Particularly, we predicted that voice would have a positive effect on task performance when communication skills were high whereas it would have a negative effect when communication skills were low. Using data collected from 299 employees of nine hospitals in Turkey through two separate surveys, we found that communication skills played a mitigating influence in which the negative relationship between voice and task performance is diminished for those employees who have higher levels of communication skills. The results suggest that the value of voice is beneficial to the degree to which employees also have high communication skills, whereas low communication skills diminish task performance benefits and weaken job engagement advantages. This is an important contribution to the current body of knowledge on voice, as this study offers new insights into how voice behavior affects follower task performance and job engagement.