Miracle-dz/UniFAR-FTUs huggingface dataset:
Serotyping of Aeromonas species isolated from Polish fish farms in relation to species and virulence phenotype of the bacteria.

The aim of the study was to determine the relationship between serogroups, species, and virulence of Aeromonas sp. Isolates from common carp and rainbow trout were tested for species designation and virulence phenotype and then serogrouped. A total of 558 isolates were tested. The bacteria were identified to species level using PCR-RFLP method. The β-haemolysin, gelatinase, and caseinase activities were selected for virulence determination. The following species were dominant: A. hydrophila (35), A. bestiarum (103), A. salmonicida (98), A. sobria (101), A. veronii bt. sobria (171), and A. encheleia (30). 380 isolates were classified as virulent for fish. The isolates were serogrouped by agglutination tests according to the scheme of Sakazaki and Shimada. 478 isolates were serologically typeable (they did not show R type antigen or autoagglutination) and for 419 (87.6%) O-antigen was identified. The dominant serogroups among both carp and trout isolates were: O:11, O:16, O:18, O:33, PGO1, and PGO2. Groups O:3, O:6 O:41, PGO4, and PGO6 dominated among carp isolates and groups O:21, O:29, PGO5, and PGO9 were only represented by trout isolates. The relationship between Aeromonas serogroups and species was not found. Of the 15 dominant serogroups, eight groups included only isolates with virulence phenotype and two groups included only non-pathogenic isolates. The remaining groups were represented by virulent, as well as non-virulent isolates. Agglutination test can be used as alternative or complementary method to differentiate pathogenic and non-pathogenic isolates from carp and trout cultured in Poland.
Dual anti-platelet therapy after coronary drug-eluting stent implantation and surgery-associated major adverse events

Summary Surgery may necessitate interruption of dual antiplatelet therapy (DAPT) within the first year after coronary drug-eluting stent (DES) implantation. We conducted a population-based cohort study to assess the rate of surgery within the first year after DES implantation, surgery-associated major adverse cardiac events (MACE), reoperation for bleeding within 30 days after surgery, and two nested case-control analyses to explore any association between preoperative antiplatelet therapy, MACE, and reoperation for bleeding. In the cohort of 22,654 patients treated with DES, 1,944 patients (8.6 %) underwent moderate- to high-risk surgery within 12 months. Of these, 62 (3.2 %) experienced MACE and 54 (2.8 %) needed reoperation for bleeding within 30 days. In the nested case-control analyses of 458 cases and controls, where 70 % (n=324) had a first generation DES, absence of preoperative antiplatelet therapy was associated with an increased MACE rate (OR 2.36, 95 % CI 1.02–5.48) compared to single antiplatelet therapy (SAPT) or DAPT. Preoperative SAPT versus DAPT showed no difference in MACE rates (OR 0.85, 95 % CI 0.30–2.40). Surgery within the first month was associated with increased MACE rate (OR 4.67, 95 % CI 2.22–9.83) compared to surgery 2–12 months after DES implantation. Absence of preoperative antiplatelet therapy did not reduce reoperation for bleeding as compared to patients on SAPT or DAPT (OR 1.32, 95 % CI 0.56–3.12). In conclusion, absence of preoperative antiplatelet therapy and surgery within the first month after DES implantation were associated with increased MACE rates.
Epithelial stem cells in corneal regeneration and epidermal gene therapy

Regenerative medicine refers to innovative therapies aimed at the permanent restoration of diseased tissues and organs. Regeneration of self‐renewing tissues requires specific adult stem cells, which need to be genetically modified to correct inherited genetic diseases. Cultures of epithelial stem cells permanently restore severe skin and mucosal defects, and genetically corrected epidermal stem cells regenerate a normal epidermis in patients carrying junctional epidermolysis bullosa. The keratinocyte stem cell is therefore the only cultured stem cell used both in cell therapy and gene therapy clinical protocols. Epithelial stem cell identification, fate and molecular phenotype have been extensively reviewed, but not in relation to tissue regeneration. In this paper we focus on the localization and molecular characterization of human limbal stem cells in relation to corneal regeneration, and the gene therapy of genetic skin diseases by means of genetically modified epidermal stem cells. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Electrodeposited Nanolaminated CoNiFe Cores for Ultracompact DC–DC Power Conversion

Laminated metallic alloy cores (i.e., alternating layers of thin film metallic alloy and insulating material) of appropriate lamination thickness enable suppression of eddy current losses at high frequencies. Magnetic cores comprised of many such laminations yield substantial overall magnetic volume, thereby enabling high-power operation. Previously, we reported nanolaminated permalloy (Ni80Fe20) cores based on a sequential electrodeposition technique, demonstrating negligible eddy current losses at peak flux densities up to 0.5 T and operating at megahertz frequencies. This paper demonstrates improved performance of nanolaminated cores comprising tens to hundreds of layers of 300-500-nm-thick CoNiFe films that exhibit superior magnetic properties (e.g., higher saturation flux density and lower coercivity) than permalloy. Nanolaminated CoNiFe cores can be operated up to a peak flux density of 0.9 T, demonstrating improved power handling capacity and exhibiting 30% reduced volumetric core loss, attributed to lowered hysteresis losses compared to the nanolaminated permalloy core of the same geometry. Operating these cores in a buck dc-dc power converter at a switching frequency of 1 MHz, the nanolaminated CoNiFe cores achieved a conversion efficiency exceeding 90% at output power levels up to 7 W, compared to an achieved permalloy core conversion efficiency below 86% at 6 W.
Prevalence and characterization of hippurate-negative Campylobacter jejuni in King County, Washington

A total of 593 strains of thermophilic Campylobacter species were isolated either from humans with diarrhea or from poultry in King County, Washington. Of these strains, 98 (52 hippurate-positive strains and all 46 of the hippurate-negative strains) were selected for further phenotypic characterization and genetic classification. Hippurate hydrolysis, the test typically used to differentiate Campylobacter jejuni and C. coli, did not always correlate with the genetic classification. All hippurate-positive strains were classified as C. jejuni. Of the hippurate-negative strains, 20% were C. jejuni, 78% were C. coli, and 2% were C. laridis. Assuming that the remaining hippurate-positive strains were all C. jejuni, then hippurate-negative C. jejuni represented a small percentage (9 of 556 or 1.6%) of C. jejuni strains but a significant percentage (9 of 46 or 20%) of hippurate-negative strains. This finding suggests that hippurate hydrolysis should not be used as the sole criterion for differentiating thermophilic Campylobacter species, particularly when describing the disease states associated with these organisms.
Two-dimensional anti-jamming communication based on deep reinforcement learning

In this paper, a two-dimensional anti-jamming communication scheme for cognitive radio networks is developed, in which a secondary user (SU) exploits both spread spectrum and user mobility to address jamming attacks, while not interfering with primary users. By applying a deep Q-network algorithm, this scheme determines whether to recommend that the SU leave an area of heavy jamming and chooses a frequency hopping pattern to defeat smart jammers. Without knowing the jamming model and the radio channel model, the SU derives an optimal anti-jamming communication policy using Q-learning in a proposed dynamic game, and applies a deep convolution neural network to accelerate the learning speed with a large number of frequency channels. The proposed scheme can increase the signal-to-interference-plus-noise ratio and improve the utility of the SU against cooperative jamming, compared with a Q-learning-only based benchmark system.
Si/SiGe-Based Photodiode on a Standard Silicon Substrate for 10-Gbit/s Short-Reach Fiber Communication at 830nm Wavelength

We report a Si/SiGe-based vertical-illuminated photodiode at 830 nm wavelength. Wide 3-dB bandwidth (>10 GHz), high responsivity (1.38 A/W), and high output current (2.35 mA) under avalanche operation can be achieved simultaneously without using silicon-on-insulator (SOI) substrate.
The Development and Neural Bases of Memory Functions as Indexed by the AB and Delayed Response Tasks in Human Infants and Infant Monkeys a

One of the classic markers of developmental change in infants between 7%-12 months of age is improved performance on “the a task” (pronounced “A not B”). This task was first devised by Piaget (1954 [1937]) and has been used by researchers throughout the world to study psychological development in babies (for reviews see Gratch, 1975; Schuberth, 1982; Harris, 1986; Wellman, Cross & Bartsch, 1987). One of the most useful tasks in the study of brain-behavior relations is a task called “delayed response. ” It was first introduced for this purpose by Jacobsen (1935; 1936) and has been widely used ever since to study brain function in monkeys and other animals (for reviews see Nauta, 1971; Rosvold, 1972; Rosenkilde, 1979; Fuster, 1980). Delayed response has been particularly useful in this regard because success on the task has been systematically linked to proper functioning of a discrete neural circuit that comprises dorsolateral prefrontal cortex and the structures with which it is interconnected. As it turns out, the a and delayed response tasks are very similar, even though they were developed independently and for almost 50 years scientists working with one task did not know of the work of scientists with the other. Indeed, it has recently been established that infants show the same developmental progression on delayed response as they show on AB (Diamond & Doar, 1989), and success on a depends on functioning of the same neural circuit as does success on delayed response (Diamond & Goldman-Rakic, 1989).
Adverse events experienced while transferring the critically ill patient from the emergency department to the intensive care unit

Objectives: To determine the incidence and nature of adverse events and delay to patient transfer from emergency department to intensive care unit (ICU) in a metropolitan tertiary hospital. Method: A 6-month prospective observational study in conjunction with a retrospective chart audit on all emergency department patients admitted to ICU, including those admitted via theatre or after a computed tomography scan. Results: Equipment problems was the most common adverse event occurring in 9% of patient transfers (n = 290). Hypothermia events occurred in 7% of transfers, cardiovascular events in 6% of patient transfers, delays to transfer >20 min occurred in 38% of the prospectively audited cases, with 14% waiting >1 h. One patient was found to have an incorrect patient identification band during a preoperative check. Conclusions: This study generally reported lower rates of adverse events than noted in previous studies involving critically ill transfers. The most significant finding was the application of an incorrect patient identification band and has prompted a review of practice. The establishment of benchmark indicators for adverse events and delays in transfer will be useful for future audits.
Vitamin K2 inhibits the growth and invasiveness of hepatocellular carcinoma cells via protein kinase A activation

Heatocellular carcinoma (HCC) is a common human malignancy. Its high mortality rate is mainly a result of high intrahepatic recurrence and portal venous invasion (PVI). We previously reported that the development of PVI is related to levels of des‐gamma‐carboxy prothrombin (DCP), a serum protein that increases at a notably higher rate in patients with HCC. Because DCP is produced by a vitamin K shortage, we examined the biological effects of extrinsic supplementation of vitamin K2 in HCC cells in vitro and in vivo. Consequently, vitamin K2 inhibits the growth and invasion of HCC cells through the activation of protein kinase A, which modulates the activities of several transcriptional factors and inhibits the small GTPase Rho, independent of suppression of DCP. In addition, administration of vitamin K2 to nude mice inoculated with liver tumor cells reduced both tumor growth and body weight loss. In conclusion, similar to an acyclic retinoid—which was previously reported to prevent the recurrence of HCC—vitamin K2, another lipid‐soluble vitamin, may be a promising therapeutic means for the management of HCC. Supplementary material for this article can be found on the HEPATOLOGY website (http://interscience.wiley.com/jpages/0270‐9139/suppmat/index.html). (HEPATOLOGY 2004;40:243–251.)
Would you prefer to use surface anatomy based on living persons or cadavers?

We thank Stern (2016) for his interest in our studies of surface anatomy based on computed tomography (CT) scans in living adults. We note his concern about the potential variability of surface anatomical features caused by different degrees of inspiration. We agree that deep inspiration or expiration will affect the position of some viscera in relation to bony landmarks and we have repeatedly acknowledged this in several of our publications (Mirjalili et al., 2012a,2012b,2012c). However, we wish to make several points:
Linkage analysis and molecular haplotyping of the dopamine D4 receptor gene promoter region

Objectives Polymorphic regions of the dopamine D4 receptor gene and its promoter region are in the focus of psychogenetic association studies. Besides the accurate phenotype characterization, highly reliable genotyping methods are also of outstanding importance in these works. Methods DNA samples of 598 healthy unrelated Caucasian individuals were used to validate the described molecular haplotyping methods and to determine the allele, genotype and haplotype frequencies and the linkage disequilibrium between the polymorphisms of the dopamine D4 receptor promoter region. Results We described a double genotyping system for the −521CT and −616CG polymorphisms, using a polymerase chain reaction restriction fragment length polymorphism or an allele-specific amplification. Allele and genotype frequencies of the novel −615AG single-nucleotide polymorphism are also determined (−615G=13.21%). For molecular haplotyping of the three single-nucleotide polymorphisms and a 120-bp duplication polymorphism, the allele-specific amplification was combined with restriction digestion. The results of the elaborated haplotyping methods were validated by molecular haplotyping of cloned fragments. Conclusions The developed methods have been arranged into an ‘economic’ protocol that might be extended for higher reliability with a double haplotyping (‘full mode’). Despite the close proximity of these sites, only a moderate linkage was found between the −615AG and −616CG (Δ2=0.162), between the −616AG and −521CT (Δ2=0.0221) and between the −615AG and −521CT single-nucleotide polymorphisms (Δ2=0.0346). The 120-bp duplication was shown to be in linkage equilibrium with any of the three single-nucleotide polymorphisms. Applications of these results should accelerate psychogenetic association studies of the dopamine D4 receptor gene.
Expression analysis of AK 003491 , an imprinted noncoding RNA , during mouse development

The Dlk1-Dio3 imprinted domain on mouse chromosome 12qF1 contains three paternally expressed protein-coding genes and multiple maternally expressed long or short noncoding RNA genes. All these imprinted genes are regulated by IGDMR located between Dlk1 and Meg3/Gtl2. Recently, several novel imprinted noncoding RNAs were identified in the intergenic region of this domain, although the exact number of imprinted genes within the region is unclear. Here, we report that a novel noncoding RNA, AK003491, located between Meg3/Gtl2 and Meg8, is maternally expressed in E15.5 brain, tongue, heart, lung, liver and kidney tissues. In situ hybridization analysis at E15.5 shows AK003491 is predominantly expressed in forebrain, tongue, thymus, somites, lung and liver. Quantitative real-time RT-PCR analysis confirms this expression pattern and detects highest expression in tongue. While the AK003491 expression pattern at postnatal day 1 is similar to E15.5, AK003491 expression at postnatal day 30 is mainly restricted to the brain. These results expand the number of known imprinted long noncoding RNAs in this domain, and contribute to further investigation of their regulatory mechanism and function.
Ethnobotanical study of some plants used in traditional medicine in the region of Oued Righ (Algerian Sahara)

The present study aims to census the ethnomedicinal plants occurring in the local traditional medicine of Oued Righ region in the Algerian Sahara. The information on medicinal uses of plants is based on a field survey, interviews, structured questionnaires and discussion with traditional healers and knowledgeable. This investigation made it possible to inventory 53 species belonging to 30 botanical families. Chenopodiaceae is the most dominant family that was used to treat various illnesses with a rate of 23.33 %; followed by the family of Asteraceae and Fabaceae with 16,66 %, Poaceae and Zygophyllaceae both with a rate of 13,33 %. These medicinal plants are prepared in various forms such as decoction, infusion, compress, inhalation, ointment, herb tea; which are made from sheets, stems, flowers, boughs, fruits, roots. The dominant diseases that can be treated with more than two plants are; Indigestion, constipation, belly and stomach pain; Injury, wounds and skin diseases; diarrhea.
A CMOS single photon avalanche diode array for 3D imaging

An 8x4 avalanche diode array in a 0.8/spl mu/m CMOS process uses single photon counting for time-of-flight range finding with 100ps 40mW decollimated laser pulses. An accuracy of 618/spl mu/m is achieved from 15cm to 1 m with 10/sup 4/ pulses.
Early and late volume changes during erythroid differentiation of cultured friend leukemic cells

Friend erythroleukemic cells (FLC) can be induced to differentiate in vitro by addition of dimethylsulfoxide (DMSO). We have studied the kinetics of induction by measuring cell volume, volume coefficient of variation and cell doubling time. Two distinct volume changes (early and late) are observed after the addition of the inducing agent. The early change occurs after ten hours and consists of a 10–20% decrease in volume compared to an untreated control population. This shift persists for two days and its magnitude is proportional to both the concentration of DMSO and the number of differentiated cells seen on day 5. FLC lines which induce weakly or not at all with DMSO exhibit a reduced or absent early volume shift. Inclusion of a local anaesthetic in the culture prevents the appearance of differentiated cells and also counteracts the early volume shift. The exact time of the early volume change is a function of cell growth rate and appears to be cell cycle related. Synchronized cell populations exposed to DMSO during G2 and S phase undergo one round of mitosis before expression of the volume change whereas cells in G2‐M express the change only after a second mitosis.
Fatal Digoxin‐Quinidine Interaction in an Elderly Woman

The medical records of an elderly woman revealed strong evidence of a lethal digoxin‐quinidine interaction. This case and several problems contributing to digitalis toxicity are presented. Physicians should be more aware of the potential toxicities involved in cardioactive drug therapy in the elderly. Current concepts of the quinidine‐digoxin interaction are discussed.
Mechanisms underlying priming on perceptual tests.

Four experiments examined perceptual, lexical, and conceptual processing effects in priming on word fragment completion (WFC) and perceptual identification (PID). In Experiment 1, visual words produced more priming than auditory or generated words, and pictures produced the least priming, suggesting that the effects of different encoding processes can be distinguished. In Experiments 2 and 3, Ss studied anagrams (e.g., tripocs), but only Ss instructed to think of the original words by mentally interchanging the vowels exhibited significant priming. Thus, lexical access is more important than surface similarity in priming. In Experiment 4, Ss studied compounds that either preserved the target's meaning (e.g., scotch bottle) or altered its meaning (e.g., scotch tape). Encoded meaning affected priming on WFC but not on PID, suggesting that conceptual processing plays a larger role in WFC. Overall the results suggest that priming must be understood in terms of multiple processes.
Variables associated with hospitalization decisions by emergency psychiatrists after a patient's suicide attempt.

OBJECTIVE This study explored the factors psychiatrists considered in deciding on hospitalization for a large sample of suicide attempters in the emergency department of a general hospital in Madrid, Spain, between 1996 and 1998. METHODS Psychiatrists assessed 509 patients who had attempted suicide; 196 of these (39 percent) were hospitalized in the psychiatric unit, and 313 (61 percent) were discharged from the emergency department. The assessment included Beck's Suicidal Intent Scale (SIS) and a checklist of 47 clinical variables. RESULTS Of the 32 clinical variables significantly associated with hospitalization that were introduced in a logistic regression model, 11 remained significant. Six variables were associated with an increased odds of hospitalization: intention to repeat the attempt, plan to use a lethal method, low psychosocial functioning before the suicide attempt, previous psychiatric hospitalization, a suicide attempt in the past year, and planning that nobody would try to save their life after they had attempted suicide. Five variables decreased the odds: a realistic perspective on the future after the attempt, relief that the attempt was not effective, availability of a method to kill oneself (that was not used), belief that the attempt would influence others, and family support. Models based on the SIS total score and individual SIS items had lower specificity and sensitivity. CONCLUSIONS Psychiatrists appear to rely on patients' self-report in deciding on hospitalization rather than focus on demographic, diagnostic, or psychosocial issues. If the findings of this study were replicated in other hospital settings, the implication would be that the guidelines for assessing suicide attempts need to encourage thorough and detailed assessment of the attempt and the future plans.