Miracle-dz/UniFAR-FTUs huggingface dataset:
A new implant with solid core and porous surface: the biocompatability with bone.

This research investigated osteogenic potencies of Farthing-Fray-Chen Titanium (FFcTi) implant with transitional porous-solid structure. The material characteristics, biomechanical property, osteogenic performances were assessed. FFcTi showed similar roughness as sand-blasted and acid etched titanium (SA), but was more hydrophilic than SA and machined commercial pure titanium (MA). Young's modulus of FFcTi implant in compressive tests was 15.8 ± 6.3 GPa, which was close to bone. In vitro observations manifested excellent spreading abilities of MC3T3-E1 cell on FFcTi and SA. Adhesion rates of MC3T3-E1 cells at 4 h gradually decreased on MA, SA, and FFcTi surfaces (MA > SA, p < 0.01; SA > FFcTi, p < 0.05), while cell proliferation ability on FFcTi was weaker than MA during 1-6 days (p < 0.01) and similar to MA and SA in day 11. ALP activity of cells on FFcTi at 14 day was higher than MA and lower than SA (p < 0.01). In a bone defect model of rabbits, BIC and bone volum ratio within 50 μm were significantly higher for FFcTi than MA (BIC, p < 0.01; BT0.05, p < 0.05) while bone volume ratio within 100 and 500 μm were of no differences. Micro CT analysis also showed similar results to the histomorphometric data. Thus, we conclude that FFcTi with melting sphere based multiporous structure has a hydrophilic, rough surface, and close modulus to bone. In vitro, its low proliferation and ALP activity promotion were similar to other micro scale roughed surface. In vivo test showed better osteogenesis ability when compared with MA at least in 2 weeks. Thus, this Farthing-Fray-Chen Titanium implant seems to hold considerable potential for bone implant applications.
Agents that increase tyrosine phosphorylation activate a non‐selective cation current in single rabbit portal vein smooth muscle cells

1 The effects of agents that increase tyrosine phosphorylation were studied with whole‐cell recording of membrane currents in single smooth muscle cells from the rabbit portal vein. 2 In K+‐free conditions with the chloride equilibrium potential at about ‐50 mV, intracellular application via the patch pipette of 200 μM sodium orthovanadate (Na3VO4), which inhibits tyrosine phosphatases, activated a ‘noisy’ inward current at a holding potential of ‐50 mV. 3 Intracellular dialysis with 100 μM (pY)EEI, a peptide activator of the cytosolic tyrosine kinase pp60c‐src, and bath application of 5 μM insulin, which activates receptor‐coupled tyrosine kinases, also evoked a ‘noisy’ inward current. The current‐voltage relationships and the reversal potential (about +10 mV) of the Na3VO4‐, pp60c‐src‐ and insulin‐induced currents were similar to those of the noradrenaline‐evoked non‐selective cation current (Icat). 4 The inward currents evoked by noradrenaline, Na3VO4, (pY)EEI and insulin were all greatly potentiated when the bathing calcium concentration was reduced from 1.5 mM to 50 μM. 5 The single channel conductance estimated from spectral density analysis of the whole‐cell current was about 20 pS for noradrenaline, Na3VO4, (pY)EEI and insulin. Moreover for all agents the spectra were described by the sum of two Lorentzians with similar corner frequencies. 6 Noradrenaline‐evoked Icat was inhibited to a similar degree by the tyrosine kinase inhibitors genistein and tyrphostin 23 and their inactive analogues daidzein and tyrphostin A1, respectively. 7 In the presence of Na3VO4, application of noradrenaline evoked a cation current of similar peak amplitude to control Icat although the rate of decay of Icat was enhanced in the presence of Na3VO4. 8 This study shows that stimulation of both cytosolic and receptor‐coupled tyrosine kinases evokes a non‐selective cation current and the conductance is similar to that activated by noradrenaline.
Psychiatric-Related Emergency Department Visits Among Children With an Autism Spectrum Disorder

Objective This study aimed to examine the prevalence and characteristics of psychiatry-related emergency department (ED) visits among children with an autism spectrum disorder (ASD), including the specific reason for visit, as well as the influence of insurance type. Methods Data used for this cross-sectional, observational study were obtained from the 2008 National Emergency Department Sample, the largest all-payer ED database in the United States. Psychiatry-related visits to the ED among children with ASD were identified using International Classification of Diseases, Ninth Revision, billing codes. A total of 3,974,332 visits (unweighted) were present for youth 3–17 years, of which 13,191 involved a child with ASD. Results Thirteen percent of visits among children with ASD were due to a psychiatric problem, as compared with 2% of all visits by youths without ASD. Results from the multivariate analyses revealed that the likelihood for a psychiatric ED visit was increased 9-fold (odds ratio [OR], 9.13; 95% confidence interval [CI], 8.61–9.70) among pediatric ASD visits, compared with non-ASD visits. Children with ASD who were covered by private insurance, compared with those with medical assistance, were at even greater risk for a psychiatric ED visit (OR, 1.58; 95% CI, 1.53–1.63). Visits among children with ASD were more likely to be due to externalizing (OR, 1.62; 95% CI, 1.44–1.83) and psychotic (OR, 1.93; 95% CI, 1.58–2.35) disorders compared with visits among non-ASD children. Conclusions This study highlights the need for improving community-based psychiatric systems of care for youths with ASD to divert psychiatry-related ED visits, particularly for those children with private insurance.
The effects of the pericardium on length-dependent regulation of left ventricular function in coronary artery surgery patients.

OBJECTIVE To analyze the effects of the pericardium on the length-dependent regulation of myocardial function in coronary artery surgery patients. DESIGN Prospective. SETTING University hospital. PARTICIPANTS Patients scheduled for elective coronary artery surgery. INTERVENTIONS In 10 patients, a combined micromanometer transducer conductance catheter was inserted into the left ventricle for measurement of left ventricular pressures and volumes. MEASUREMENTS AND MAIN RESULTS Consecutive data were obtained during a progressive increase in left ventricular pressures and volumes obtained by leg elevation in closed chest-closed pericardium and open chest-open pericardium conditions. Pericardiotomy did not alter baseline left ventricular hemodynamics. The effects of leg elevation were different, however. In closed chest-closed pericardium conditions, stroke volume and stroke work remained unchanged, whereas these parameters increased in open chest-open pericardium conditions. This increase was related to the increase in end-diastolic volume that was observed in open chest-open pericardium conditions and not in closed chest-closed pericardium conditions. CONCLUSIONS In coronary artery surgery patients, pericardiotomy does not alter baseline left ventricular function. When cardiac load is increased by leg elevation, however, use of the Frank-Starling mechanism is enhanced in open chest-open pericardium conditions.
19q13 amplification is associated with high grade and stage in pancreatic cancer

Pancreatic cancer is a devastating disease with an extremely poor prognosis, and thus, there is a great need for better diagnostic and therapeutic tools. The 19q13 chromosomal locus is amplified in several cancer types, including pancreatic cancer, but the possible clinical significance of this aberration remains unclear. We used fluorescence in situ hybridization on tissue microarrays containing 357 primary pancreatic tumors, 151 metastases, and 24 local recurrences as well as 120 cancer cell lines from various tissues to establish the frequency of the 19q13 amplification and to find potential correlations to clinical parameters including patient survival. Copy number increases were found in 12.2% of the primary pancreatic tumors and 9.3% of the cell lines, including those derived from bladder, colorectal, ovarian, and thyroid carcinomas. Copy number changes were linked to high grade (P = 0.044) and stage (P = 0.025) tumors, and the average survival time of patients with 19q13 amplification was shorter than that of those without this aberration. Our findings revealed recurrent 19q13 amplification in pancreatic cancer and involvement of the same locus as in bladder, colorectal, ovarian, and thyroid carcinomas. More importantly, the 19q13 amplifications were associated with poor tumor phenotype and showed a trend toward shorter survival. © 2010 Wiley‐Liss, Inc.
Outcome of “Atypical squamous cells” in a cervical cytology screening program: Implications for follow up in resource limited settings

(1) To study the incidence and outcome of “Atypical squamous cells (ASC)” diagnosis in a hospital based cytology screening programme. (2) To work out a feasible strategy for follow up of Atypical squamous cells–undetermined significance (ASC‐US) and Atypical squamous cells–cannot exclude high grade squamous intraepithelial lesion (ASC‐H) in resource limited settings. A total of 29,475 women were screened cytologically through Pap tests. The epithelial cell abnormalities (ECA) detected on screening were reviewed by the cytopathologists and classified according to Bethesda 2001 system. The women with ASC‐US reports were followed up by two repeat cytology tests at 3 and 6 months of the initial visit. The persistent ASC‐US cases or the cases which revealed squamous intraepithelial lesion (SIL) on follow up smears; as well as all ASC‐H and above cases were referred for coloposcopic evaluation. ASC diagnosis comprised 3.6% of all reports. These were qualified as ASC‐US (3.36%) and ASC‐H (0.22%). On follow up, only 7 CIN 2 or worse (3.2%) lesions were detected on 218 biopsies in ASC‐US category while in ASC‐H qualifier 16 CIN 2 and above lesions (30.8%) were picked up on 52 colposcopic biopsies. The difference between ASC‐US and ASC‐H categories for a CIN 2 or worse outcome was highly significant (P < 0.001). ASC‐H qualifier has a high likelihood for an ominous histological outcome and warrants an immediate colposcopic evaluation. On the other hand, ASC‐US cases can be managed conservatively by repeat cytology tests at regular intervals without a significant risk of missing a high grade lesion. Diligent screening of cervical smears can judiciously downgrade some cases overcalled as ASC because of inflammatory atypia and thereby reduce referrals in geographic settings with high prevalence of reproductive tract infections. High risk HPV (HR HPV) testing may be a useful adjunct to further reduce referrals by selecting the women who require colposcopic evaluation. Diagn. Cytopathol. 2007; 35:677–680. © 2007 Wiley‐Liss, Inc.
Intra-articular steroid hip injection for osteoarthritis: a survey of orthopedic surgeons in Ontario.

BACKGROUND Intra-articular steroid hip injection (IASHI) has been prescribed for painful hip arthritis since the 1950s, but with advances in medical and surgical management its role is less certain today. There are very few published data on the utility or prescribing patterns of IASHI. METHODS We developed a questionnaire to seek expert opinion on IASHI that we distributed to practising Ontario-based members of the Canadian Orthopaedic Association. We systematically describe the current practices and expert opinion of 99 hip surgeons (73% response rate), focusing on indications, current use and complications experienced with IASHI. RESULTS Only 56% of surgeons felt that IASHI was therapeutically useful, with 72% of surgeons estimating that 60% or less of their patients achieved even transient benefit from IASHI. One-quarter of the surgeons believe that IASHI accelerates arthritis progression, most of whom had stated that it would be no great loss if IASHI was no longer available. Nineteen percent of the surgeons believed that the infection rate related to total hip arthroplasty (THA) may be increased after IASHI, and this was associated with fewer IASHIs ordered per year, compared with the number prescribed by those who did not feel that infection rates would increase. CONCLUSIONS This systematic collection of expert opinions demonstrates that substantial numbers of surgeons felt that, in their patients, IASHI was not therapeutically helpful, may accelerate arthritis progression or may cause increased infectious complications after subsequent THA.
Psychotherapy and close‐process technique

The applicability and advantages of Paul Gray’s concept of close‐process in the practice of psychotherapy are discussed. Gray seems to have underestimated the potential and versatility of his technical approach to derivative psychotherapy procedures. Clinical vignettes are provided, describing some nodal points for intervention, and exploration of transferential, extratransferential and genetic aspects of the workable surface. Gray’s methodology of intraclinical attention permits a more verifiable examination of sequences, an issue of special relevance in our age of empirical standards for the evaluation of dynamic therapies.
Autoradiographic localization of aromatic hydrocarbon receptor (AHR) in rhesus monkey ovary

2,3,7,8‐Tetrachlorodibenzo‐p‐dioxin (TCDD) is the most toxic congener of a large class of manmade pollutants that persist in the environment. TCDD exerts its toxic effects, in part, by binding to its receptor known as the aromatic hydrocarbon receptor (AHR). TCDD is estrogen modulatory and in some systems its receptor associates directly with estrogen receptors via co‐activator molecules. TCDD inhibits steroid synthesis in human ovarian granulosa cells and AHR is found in these cells. We have previously shown that AHR is found in whole rhesus monkey ovary, but have yet to establish its location. In the present study, we set out to show that radiolabeled TCDD binds to monkey ovarian follicles and that this binding is receptor mediated. Ovaries from Macaca mulatta were sectioned on a cryostat at 10 µ m; and sections were incubated with either control vehicle, 3H‐TCDD, or 3H‐TCDD plus alpha‐naphthoflavone (ANF), a known receptor‐blocking agent. Here, we show for the first time specific binding of TCDD to the granulosa cells of antral follicles and other regions of the rhesus monkey ovary. Our data indicate a 60‐fold increase in binding with 3H‐TCDD over that of control, and that this binding is reduced to the levels seen in controls with the addition of the competitive antagonist ANF. These findings support the hypothesis that TCDD directly affects primate ovarian function via the AHR. Am. J. Primatol. 69:681–691, 2007. © 2006 Wiley‐Liss, Inc.
Body Mass Normalization for Isometric Tests of Muscle Endurance

Abstract Nuzzo, JL and Mayer, JM. Body mass normalization for isometric tests of muscle endurance. J Strength Cond Res 27(7): 2039–2045, 2013—The primary purpose of this study was to derive allometric parameters for normalizing Biering-Sorensen and Plank Test scores (measures of isometric back and abdominal muscle endurance, respectively) to body mass. The secondary purpose was to determine if allometric scaling with derived allometric parameters or the theoretical allometric parameter (b = −0.33) is the more appropriate method for normalizing Biering-Sorensen and Plank Test scores to body mass. A sample of 83 male career firefighters (age = 35.7 ± 9.4 years; body mass = 89.5 ± 14.8 kg) participated in this study. Significant correlations were found between body mass and test scores (Biering-Sorensen Test: r = −0.36, p = 0.001; Plank Test: r = −0.55, p < 0.001). Derived allometric parameters for Biering-Sorensen and Plank Tests were b = −0.932 and −1.369, respectively. Allometric scaling with the derived allometric parameters was deemed appropriate at normalizing Biering-Sorensen and Plank Test scores because it caused the correlations between body mass and test scores to become insignificant (Biering-Sorensen Test: r = 0.10, p = 0.354; Plank Test: r = 0.14, p = 0.173). Allometric scaling with the theoretical allometric parameter was deemed inappropriate for normalizing Biering-Sorensen and Plank Test scores because it was not robust enough to get rid of the significant correlations between body mass and test scores (Biering-Sorensen Test: r = −0.23, p = 0.034; Plank Test: r = −0.43, p < 0.001). Allometric scaling with derived allometric parameters should be used to normalize Biering-Sorensen and Plank Test scores to body mass, particularly when attempting to account for changes in body mass across time intervals and differences in body mass across individuals.
Calcium dynamics during NMDA‐induced membrane potential oscillations in lamprey spinal neurons – contribution of L‐type calcium channels (CaV1.3)

• Intrinsic NMDA‐induced oscillations of membrane potential are important for the operation of the spinal locomotor network in the lamprey, and involve a cyclic influx of calcium. • The spatial and temporal calcium dynamics during oscillations were investigated, using calcium imaging combined with intracellular recordings. • Calcium fluctuations were observed in both soma and dendrites, timed to the membrane potential oscillations, with the peak in distal dendritic locations typically occurring earlier than in the soma. • The L‐type calcium channel antagonist nimodipine prolonged the plateau phase of membrane potential oscillations, whereas the agonist Bay K 8644 shortened the plateau. Bay K 8644 also increased the amplitude of calcium fluctuations, particularly in distal dendrites. On the contrary, nimodipine decreased calcium fluctuation amplitudes. • Low‐voltage‐activated L‐type (CaV1.3‐like) calcium channels at distal dendrites are activated during membrane potential oscillations. The resulting calcium influx contributes to the activation of calcium‐activated potassium channels and thereby the control of the plateau duration during oscillations.
Carbon isotope minima in the South Atlantic during the last deglaciation: evaluating the influence of air-sea gas exchange

Carbon isotope minima were a ubiquitous feature in the mid-depth (1.5–2.5 km) Atlantic during Heinrich Stadial 1 (HS1, 14.5–17.5 kyr BP) and the Younger Dryas (YD, 11.6–12.9 kyr BP), with the most likely driver being collapse of the Atlantic Meridional Overturning Circulation (AMOC). Negative carbon isotope anomalies also occurred throughout the surface ocean and atmosphere, but their timing relative to AMOC collapse and the underlying drivers have remained unclear. Here we evaluate the lead-lag relationship between AMOC variability and surface ocean δ13C signals using high resolution benthic and planktonic stable isotope records from two Brazil Margin cores (located at 1.8 km and 2.1 km water depth). In each case, the decrease in benthic δ13C during HS1 leads planktonic δ13C by 800 ± 200 years. Because the records are based on the same samples, the relative timing is constrained by the core stratigraphy. Our results imply that AMOC collapse initiates a chain of events that propagates through the oceanic carbon cycle in less than 1 kyr. Direct comparison of planktonic foraminiferal and atmospheric records implies a portion of the surface ocean δ13C signal can be explained by temperature-dependent equilibration with a 13C-depleted atmosphere, with the remainder due to biological productivity, input of carbon from the abyss, or reduced air-sea equilibration.
Immunohistochemical Detection of the MUC1 Gene Product in Human Cancers Grown in scid Mice

Alterations in mucin expression have been detected in many clinically relevant cancers and, in particular, the polymorphic epithelial mucin, encoded by the MUC1 gene, has attracted considerable attention. We investigated its expression in human breast, colon, ovarian, lung, and skin cancer cells and their metastases grown in severe combined immunodeficient (scid) mice using three different monoclonal antibodies (HMFG-1, HMFG-2, and SM3). Four of five breast cancer cell lines, three of five colon cancer cell lines, two of three small-cell carcinoma of the lung cell lines, and A 431 cells all expressed the MUC1 gene product. Neuraminidase predigestion often enhanced HMFG-1 immunoreactivity, which was more widespread and stronger than SM3 immunoreactivity. A considerable heterogeneity of MUC1 gene product expression was observed in the same tumors grown in different mice. The binding pattern between single-cell/small-cell clusters (up to 10 cells) and larger cell number aggregates varied. The results indicate that the MUC1 gene expression both in primary tumors and metastases is not tightly controlled within a particular tumor cell line. Because of this heterogeneous antigen expression in vivo, it appears impossible to target all metastatic deposits by a single monoclonal antibody directed against the MUC1 gene product.
Ductal carcinoma in situ on stereotactic biopsy of suspicious breast microcalcifications: Expression of SPARC (Secreted Protein, Acidic and Rich in Cysteine) can predict postoperative invasion

Secreted protein, acidic and rich in cysteine (SPARC) is able to play an important role in cancer invasion due to de‐adhesive properties and impact on stromal remodeling. The aim of study was to investigate SPARC expression in preoperatively diagnosed breast DCIS and to assess its predictive value for the final invasion.
Romanian Branded Hotels: Are They Worth the Effort?

The presence of branded hotels in Romania remains relatively modest after two decades since the first Sofitel (Accor Hotels) entered the market. Thus, the number of branded hotels increased since 2005 when they were first investigated. The present study investigates the RevPAR of the Romanian hotels that have joined an international brand, either hard (franchising and management contracts) or soft (affiliation), and the hotel operators’ net financial results over a five year period 2012-2016. The results of this exploratory study reveal that the most notorious brands operating in Romania are also those generating the highest RevPAR. Further, the results show that operating a brand outside Bucharest (mainly a hard brand) proves to generate constantly net profit for most of the respective hotel operators, despite a RevPAR below the European average. This finding suggests that the brand notoriety generates an increased occupancy rate. The results indirectly point out that most of the branded hotels’ operators seem to struggle on the crowded Bucharest hotel market enhancing the idea of internal inefficiencies. Given the structure of the Romanian hotel industry, the partnership with an international brand seems to be worth the effort mainly if the brand is appropriately chosen taking into consideration the hotel location and size.