Miracle-dz/UniFAR-FTUs huggingface dataset:
Cognitive function in short- and long-term substitution treatment: Are there differences?

Cognitive impairment in drug-dependent patients receiving methadone maintenance treatment has been reported previously, although the literature is limited and results remain controversial. Long-term effects under stable methadone maintenance treatment (MMT) and the possibility of improvement in cognitive performance during long-term substitution treatment have rarely been investigated. We performed a comparative study investigating differences in cognitive functions under short- and long-term methadone treatment to test the hypothesis that patients perform better under long- than under short-term MMT. Seventy-seven patients were assessed cross-sectional either at least 30 days after the start of MMT (short-term group, n=35) or after at least 6 months of MMT (long-term group, n=42) with a comprehensive neuropsychological test battery on intelligence, learning and memory, attention and executive functions. Urine screenings were performed immediately before neuropsychological testing to check for concomitant drug use. Our findings may suggest, with all due caution, a slightly better performance of the long-term group in executive functions and visuo-construction. No group differences were found in attentional functions and learning and memory. More longitudinal research and studies controlling for the effects of dosage and duration of opioid addiction are necessary to examine whether cognitive ability may improve under long-term MMT.
The adenovirus death protein (E3-11.6K) is required at very late stages of infection for efficient cell lysis and release of adenovirus from infected cells

Adenovirus (Ad) infection is concluded by assembly of virions in the cell nucleus followed by lysis of cells by an unknown mechanism. We have described an Ad nuclear membrane glycoprotein of 11,600 kDa (E3-11.6K) which is encoded by the E3 transcription unit and which is synthesized in small amounts from the E3 promoter at early stages of infection but in large amounts from the major late promoter at very late stages of infection. We now report that E3-11.6K is required for the efficient lysis (death) of Ad-infected cells, and we propose that the function of E3-11.6K is to mediate the release of Ad progeny from infected cells. We have renamed E3-11.6K the Ad death protein (ADP). Virus mutants that lack ADP replicated as well as adp+ Ad, but the cells lysed more slowly, virus release from the cell was retarded, and the plaques were small and developed slowly. Cells infected with adp+ viruses began to lyse at 2 or 3 days postinfection (p.i.) and were completely lysed by 5 or 6 days p.i. In contrast, cells infected with adp mutants did not begin significant lysis until 5 or 6 days p.i. Cell lysis and viability were determined by plaque size, extracellular virus, cell morphology, release of lactate dehydrogenase, trypan blue exclusion, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay for mitochondrial activity, RNA degradation, and DNA degradation as determined by agarose gel electrophoresis and the terminal deoxynucleotidyltransferase end labeling assay. Protein synthesis was almost nonexistent at 3 days p.i. in cells infected with adp+ Ads, but it was still increasing in cells infected with adp mutants. Host cell protein synthesis was undetectable at 1 day p.i. in cells infected with adp+ Ads or adp mutants. Cells infected with adp mutants showed Ad cytopathic effect at 1 or 2 days p.i. in that they rounded up and detached, but the cells remained metabolically active and intact for >5 days p.i. When examined by electron microscopy, the nuclei were extremely swollen and full of virus, and the nuclear membrane appeared to be intact. ADP is unrelated in sequence to other known cell death-promoting proteins.
Effects of water-saving superabsorbent polymer on antioxidant enzyme activities and lipid peroxidation in oat (Avena sativa L.) under drought stress.

BACKGROUND Drought stress significantly limits oat (Avena sativa L.) growth and productivity. Thus an efficient management of soil moisture and study of metabolic changes in response to drought are important for improved production of oat. The objective was to gain a better understanding of drought tolerance mechanisms and improve soil water management strategies using water-saving superabsorbent polymer (SAP) at 60 kg ha(-1) under three irrigation levels (adequate, moderate and deficit) using a new type of hydraulic pressure-controlled auto irrigator. RESULTS The results showed that the relative water content and leaf water potential (ψ(1) ) were much higher in oats treated with SAP. Although the SAP had little effect on plant biomass accumulation under adequate and moderate irrigation, it significantly increased the biomass by 52.7% under deficit irrigation. Plants treated with SAP under deficit irrigation showed a significant decrease in superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and glutathione reductase activities in leaves compared with control plants. CONCLUSION Our results suggested that drought stress leads to production of oxygen radicals, which results in increased lipid peroxidation and oxidative stress in the plant, and the application of SAP could conserve soil water, making same available to plants for increased biomass accumulation and reduced oxidative stress especially under severe water stress.
In the wake of conflict: the long-term effect on child nutrition in Uganda

ABSTRACT The Lord’s Resistance Army (LRA) insurgency from 1986 to 2006 is one of the longest conflicts in Uganda. This paper examines the effect of the LRA insurgency on child nutrition using the 2011 Uganda Demographic and Health Survey. The distance from each village to the border of South Sudan is used as an instrument for identifying the intensity of the conflict. We find that each conflict event in a village lowers weight-for-age z-scores and weight-for-height z-scores for children born after the conflict. We further investigate a possible heterogeneous conflict effect on child nutrition in terms of gender, region, and maternal education. Low household wealth, limited access to healthcare, and poor maternal nutrition appear to be channels through which the conflict inhibits the growth and development of children 5 years after the end of the conflict.
Mycobacteria and the Intraphagosomal Environment: Take It With a Pinch of Salt(s)!

Ancient protozoan phagocytes and modern professional phagocytes of metazoans, such as macrophages, employ evolutionarily conserved mechanisms to kill microbes. These mechanisms rely on microbial ingestion, followed by maturation of the phagocytic vacuole, or so‐called phagosome. Phagosome maturation includes a series of fusion and fission events with the host cell endosomes and lysosomes, leading to a rapid increase of the degradative properties of the vacuole and to the destruction of the ingested microbe within a very hostile intracellular compartment, the phagolysosome. Historically, the mechanisms and weapons used by phagocytes to kill microbes have been separated into different classes. Phagosomal acidification, together with the production of reactive oxygen and nitrogen species, the selective manipulation of various ions in the phagosomal lumen, and finally the engagement of a battery of acidic hydrolases, are well‐recognized players in this process. However, it is relatively recently that interconnections among these mechanisms have become apparent. In this review, we will focus on some emerging concepts about these interconnected aspects of the warfare at the host–pathogen interface, using mostly Mycobacterium tuberculosis as an example of intracellular pathogen. In particular, recent discoveries on the role of phagosomal ions and other chemicals in the control of pathogens, as well as mechanisms evolved by intracellular pathogens to circumvent or even exploit the weapons of the host cell will be discussed.
Cytoarchitecture and fiber connections of the nucleus lateralis valvulae in the carp (Cyprinus carpio)

The cytoarchitecture and fiber connections of the nucleus lateralis valvulae of the carp (Cyprinus carpio) were studied by Nissl, Bodian, Golgi, and horseradish peroxidase methods. Chief cells composing the nucleus lateralis valvulae (NLV) are small and granular, and their axons terminate in the cerebellum. These neurons have no dendrite, and the cell body is enveloped in a single terminal of afferent fibers to the NLV. In order to observe local cell clustering, the NLV was three‐dimensionally reconstructed with the aid of a computer image analysis system.
No Independent Associations of LMP2 and LMP7 Polymorphisms With Susceptibility to Develop IDDM

Results from a recent study suggested that polymorphisms within the HLA class II genes LMP2 and LMP7 were associated with the susceptibility for developing IDDM, and that this association could not be explained by linkage disequilibrium to HLA-DR or -DQ genes. We typed 285 IDDM patients and 337 HLA-DRB1-DQA1-DQB1 genotypically matched control subjects from an ethnically homogeneous population for both the G/T polymorphism in intron 6 of the LMP7 gene and the Arg-His polymorphism in the LMP2 gene. In addition, we typed IDDM families in which at least one parent was homozygous for a DRB1-DQA1-DQB1 haplotype and performed a transmission/disequilibrium test of these LMP polymorphisms. Our data suggest that none of these LMP2 or LMP7 polymorphisms are independently associated with IDDM susceptibility, in contrast to what has been previously reported by others. Further, our results suggest that one partial explanation for the previously reported independent association between IDDM and these LMP polymorphisms may have been that patients and control subjects were not matched for DRB1*04 subtypes. Our results emphasize the need for a complete matching for DRB1, DQA1, and DQB1 alleles between patients and control subjects when attempting to detect independent effects of other polymorphisms in the HLA complex on IDDM susceptibility or protection.
A novel open-resonator system for precise measurement of permittivity and loss-tangent

An open-resonator system is constructed for the precision dielectric permittivity and loss-tangent measurement of low-absorbing materials at 60 GHz. In this new inexpensive simple system, only one frequency source is used together with a liquid helium-cooled InSb detector for the full cavity-length variation technique. This new simple open resonator system provides data with high measurement accuracy although it only uses one fixed frequency source.
Functional domains of business incubators for refugee entrepreneurs

Purpose The purpose of this paper is to conceptualize business incubators and their support for entrepreneurial refugees. While the number of initiatives supporting refugees’ entrepreneurial activities has increased in recent years, we still know little about how they differ from other types of business incubators. Design/methodology/approach This case study investigates a business incubator in Hamburg, Germany, targeting enterprising refugees. For this paper, 14 in-depth interviews with program participants and incubation managers were conducted. Findings This paper inductively derives five functional domains of refugee business incubators: providing structured entrepreneurial knowledge; alleviating anxiety related to institutional differences; guiding through the process at the incubator and motivating participants; understanding and tapping into social capital in the host country; and providing soft support concerning personal matters. The findings show that business incubators could and possibly should address specific needs of refugees and that there is much room for improvement. This study suggests that the five domains listed above represent key characteristics that distinguish refugee business incubators from traditional business incubators. Practical implications This paper offers valuable practical insights for refugee business incubators, which need to consider and develop functional domains listed above. Because these kinds of incubators are a fairly recent phenomenon, there is a general lack of and need for blueprints. The findings of this paper suggest that business incubators could integrate and support entrepreneurial refugees provided that they consider the five functional domains identified here. The findings also provide evidence that entrepreneurship can be a possible means of vocational integration for refugees and one way of institutions and policy-makers in host country seeking to support refugees’ entrepreneurial activities, for example, by developing or subsidizing business incubators targeting refugees. Originality/value This paper’s contributions are twofold. First, this paper addresses a gap in the literature on refugee entrepreneurship by providing insights concerning the important role of support institutions. Second, this paper conceptualizes business incubators for enterprising refugees as a distinctive type of business incubators. This paper has, however, some limitations. Because it only considered a relatively small number of refugee entrepreneurs, it is difficult to generalize the findings. The cross-cultural setting of the empirical study, with its potential for linguistic and cultural misunderstandings, may have affected the results.
COMPARING THE HTA CORE MODEL WITH A NATIONAL HEALTH TECHNOLOGY ASSESSMENT REPORT

Objectives: The HTA Core Model is a framework for producing health technology assessments (HTAs) in a structured format. The Model splits the content of a HTA into assessment elements. The objective is to explore the adaptability of these assessment elements in national report production in a pilot case study comparing a national HTA report and the HTA Core Model. Methods: An on-going Dutch HTA report on endovascular repair of abdominal aortic aneurysm (EVAR) was chosen as a typical representative of a national report on medical interventions. The author of the EVAR report assessed the relevance and comprehensiveness of the assessment elements of the HTA Core Model for her work. Another researcher annotated the Core Model specific content in the EVAR report. Matching and missing content, as well as the distribution of information in the EVAR report were tabulated and analysed in joint deliberations. Results: Forty percent of the assessment elements of the Core Model were considered relevant for the EVAR report. Some issues relevant for EVAR but missing from the Core Model were identified: they were about re-interventions, secondary prevention, subpopulations that benefit most, and the length of the hospital stay. The distribution of information differed substantially between the Code Model and the national report. Conclusions: The assessment elements of the HTA Core Model covered most relevant questions of the national report. In order to facilitate easy adaptation of information, the distribution of information should be more consistent in the national report and the Core model.
A ftir, raman and SEM - EBSD - EDS microstructure characterization of plasma thermally sprayed silicon sheet for low cost solar cells substrate

Polycrystalline silicon still guarantees its application on solar cells as important market product until year 2050. Due to the increase of photovoltaic industry needing, technologies to produce low cost silicon will be needed. The metallurgical grade silicon (Si-GM) has been used to produce Si substrates by the atmospheric plasma thermal spray (APTS) which has the advantages of a low cost and fast process. The aim of this work is to characterize the APTS Si sheet microstructure and chemical composition to promote its technology association for low cost solar cell substrate production. The raw material and APTS Si sheet were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and Raman spectroscopy. The results showed a structural mixed silicon sheet with different levels of crystalline fraction, amorphous structures contribution, phase transitions regions, crystallite size distribution as well as a mainly silicon oxides covered surface.
Placebo‐Controlled Trial of Doxazosin in Management of Patients with Hypertension and Hypercholesterolaemia

Summary: The blood pressure (BP)- and lipid-lowering activities of the α1-antagonist, doxazosin, were investigated in hypertensive, hypercholesterolaemic patients. Thirty-one patients satisfactorily completed the study, and there was no significant difference between doxazosin and placebo in terms of reported adverse events. After 3-month treatment, BP was significantly reduced by doxazosin by 24/14 mm Hg supine and by 33/22 mm Hg erect as compared with corresponding reductions of 2/9 and 2/2 mm Hg with placebo. There were concomitant improvements in the plasma lipid profile with, in particular, significant net reductions of 30% for triglycerides and 20% for apoprotein B. There was no adverse effect on glucose metabolism. The principal aim of this study was assessment of the clinical utility and acceptability of doxazosin in a heterogeneous population of patients with several cardiovascular risk factors. The results confirm that doxazosin is an effective antihypertensive agent that has modest additional beneficial effects on the plasma lipid profile.
Pancreatic cysts: depiction on single-shot fast spin-echo MR images.

PURPOSE To evaluate single-shot fast spin-echo (SE) magnetic resonance (MR) imaging for depiction of pancreatic cysts in a large number of patients and to analyze cyst prevalence with respect to patient age and sex and other clinical information. MATERIALS AND METHODS Single-shot fast SE images of the pancreas were obtained in 1,444 patients. The images were reviewed for presence of pancreatic cysts, which were classified as simple and nonsimple types, and cyst diameters were measured. kappa statistic, Fisher exact, McNemar Q, and Pearson product moment correlation tests were performed. RESULTS Two hundred eighty-three (19.6%) patients had at least one pancreatic cyst. The prevalence of pancreatic cysts increased with age (r = 0.96). The percentages of male and female patients with pancreatic cysts (20.4% vs 18.8%) were not significantly different. Two hundred seventy-one (18.8%) patients had simple cysts, and 147 (10.2%) had nonsimple cysts. Of 283 patients with pancreatic cysts, 158 (55.8%) had only one pancreatic cyst. The number of patients with multiple cysts increased with age after 70 years. Four hundred fifteen (83.8%) cysts were 10 mm in diameter or smaller; 56 (11.3%), 11-20 mm in diameter; and 24 (4.9%), 21 mm in diameter or larger. Sixteen (5.7%) patients with pancreatic cysts had malignant pancreatic tumors, and 75 (26.5%) patients had pancreatitis. CONCLUSION The prevalence of pancreatic cysts at single-shot fast SE MR imaging-especially cysts with a diameter smaller than 10 mm-is similar to that of pancreatic cysts at autopsy and higher than that of pancreatic cysts at transabdominal ultrasonography. Prevalence is especially high in patients with pancreatitis.
Cytolethal Distending Toxin from Aggregatibacter actinomycetemcomitans Induces DNA Damage, S/G2 Cell Cycle Arrest, and Caspase- Independent Death in a Saccharomyces cerevisiae Model

ABSTRACT Cytolethal distending toxin (CDT) is a bacterial toxin that induces G2/M cell cycle arrest, cell distension, and/or apoptosis in mammalian cells. It is produced by several Gram-negative species and may contribute to their pathogenicity. The catalytic subunit CdtB has homology with DNase I and may act as a genotoxin. However, the mechanism by which CdtB leads to cell death is not yet clearly understood. Here, we used Saccharomyces cerevisiae as a model to study the molecular pathways involved in the function of CdtB from Aggregatibacter actinomycetemcomitans, a cause of aggressive periodontitis. We show that A.actinomycetemcomitans CdtB (AaCdtB) expression induces S/G2 arrest and death in a DNase-catalytic residue and nuclear localization-dependent manner in haploid yeasts. Yeast strains defective in homologous recombination (HR) repair, but not other DNA repair pathways, are hypersensitive to AaCdtB, suggesting that HR is required for survival upon CdtB expression. In addition, yeast does not harbor the substrate for the other activity proposed for CdtB function, which is phosphatidylinositol-3,4,5-triphosphate phosphatase. Thus, these results suggest that direct DNA-damaging activity alone is sufficient for CdtB toxicity. To investigate how CdtB induces cell death, we examined the effect of CdtB in yeast strains with mutations in apoptotic regulators. Our results suggest that yeast death occurs independently of the yeast metacaspase gene YCA1 and the apoptosis-inducing factor AIF1 but is partially dependent on histone H2B serine 10 phosphorylation. Therefore, we report here the evidence that AaCdtB causes DNA damage that leads to nonapoptotic death in yeast and the first mutation that confers resistance to CdtB.
Caregivers’ Health Literacy and Their Young Children’s Oral-health–related Expenditures

Caregivers’ health literacy has emerged as an important determinant of young children’s health care and outcomes. We examined the hypothesis that caregivers’ health literacy influences children’s oral-health-care–related expenditures. This was a prospective cohort study of 1,132 child/caregiver dyads (children’s mean age = 19 months), participating in the Carolina Oral Health Literacy Project. Health literacy was measured by the REALD-30 (word recognition based) and NVS (comprehension based) instruments. Follow-up data included child Medicaid claims for CY2008-10. We quantified expenditures using annualized 2010 fee-adjusted Medicaid-paid dollars for oral-health–related visits involving preventive, restorative, and emergency care. We used descriptive, bivariate, and multivariate statistical methods based on generalized gamma models. Mean oral-health–related annual expenditures totaled $203: preventive—$81, restorative—$99, and emergency care—$22. Among children who received services, mean expenditures were: emergency hospital-based—$1282, preventive—$106, and restorative care—$343. Caregivers’ low literacy in the oral health context was associated with a statistically non-significant increase in total expenditures (average annual difference = $40; 95% confidence interval, -32, 111). Nevertheless, with both instruments, emergency dental care expenditures were consistently elevated among children of low-literacy caregivers. These findings provide initial support for health literacy as an important determinant of the meaningful use and cost of oral health care.