Miracle-dz/UniFAR-FTUs huggingface dataset:
Isolation and characterization of the outer membrane and lipopolysaccharide from Eikenella corrodens

The chemical composition of the outer membrane fractions (OMFs) of Eikenella corrodens strains 23834 and 470 as well as the strain 23834 lipopolysaccharide (LPS) was determined. The OMFs were obtained by Triton X-100 treatment of the heavier membrane fraction from sucrose density centrifugation of the total membrane fraction. The resulting OMFs of strains 23834 and 470, free of cytoplasmic membrane components, were found to contain 69.6 and 75.0% (wt/wt) protein, 4.8 and 9.2% lipid, 4.6 and 4.7% carbohydrate, and 2.0 and 4.6% muramic acid, respectively. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis both OMFs contained one major peptide determined to be 33,500 daltons for the strain 23834 OMF, and 37,500 daltons for the strain 470 OMF. Analysis of the OMF fatty acids revealed hexadecanoic, hexadecenoic, octadecenoic, and lesser amounts of octadecanoic acids. Transmission electron microscopic examination of the OMFs revealed typical large sheets of membrane. Structures (10 nm in diameter) resembling pores were also evident. The E. corrodens LPS was found to be composed of 34.5% (wt/wt) carbohydrate and 25.0% lipid A. Only minute amounts of 2-keto-3-deoxyoctonate and heptose could be detected. Fatty acid analysis revealed primarily octadecanoic and hexadecanoic acids, with lesser amounts of octadecenoic acid. No hydroxy fatty acids were detected. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed the E. corrodens LPS to resemble other smooth-type LPSs. Transmission electron microscopic examination revealed a vesicle-like morphology. The E. corrodens LPS appears not to be a "classical," i.e., enteric, type of LPS.
The Effects of Insertion Speed on Inner Ear Function during Cochlear Implantation: A Comparison Study

Objective: To investigate the insertion speed and its impact on electrode insertion characteristics, hearing preservation and clinical vestibular function in a prospective cohort study with a retrospective control group at a tertiary otology/neurotology centre. Interventions: Hearing-preserving cochlear implantation using systemic and topical steroids in conjunction with a round-window approach, a complete cochlear coverage electrode and two different electrode insertion speeds [60 mm/min (n = 18) vs. 15 mm/min (n = 22)] was performed. Results: The insertion speed had a significant impact on various insertion characteristics as well as hearing preservation and vestibular function. In conclusion, a slow electrode insertion speed appears to facilitate full electrode insertion, reduce the occurrence of insertion resistance as well as promote preservation of residual hearing and vestibular function after cochlear implantation.
Induction of altered hepatic foci by a mixture of dioxin-like compounds with and without 2,2',4,4',5,5'-hexachlorobiphenyl in female Sprague-Dawley rats.

The hepatic tumor-promoting activity of a mixture of polyhalogenated aromatic hydrocarbons (PHAHs) was studied in a medium term two-stage initiation/promotion bioassay in female Sprague-Dawley rats. The PHAH mixture contained 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 1, 2,3,7,8-pentachlorodibenzo-p-dioxin (PeCDD), 2,3,4,7, 8-pentachlorodibenzofuran (PeCDF), 3,3',4,4',5-pentachlorobiphenyl (PCB 126), 2,3',4,4',5-pentachlorobiphenyl (PCB 118), 2,3,3',4,4', 5-hexachlorobiphenyl (PCB 156), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153) and covered >90% of the total toxic equivalents (TEQ) present in Baltic herring. To determine possible interactive effects of di-ortho-substituted PCBs, the PHAH mixture was tested with (PHAH+) and without (PHAH-) PCB 153. Rats were initiated by a diethylnitrosamine injection (30 mg/kg body wt i.p.) 24 h after a partial 23 hepatectomy. Six weeks after initiation, the PHAH mixtures were administered once a week by subcutaneous injections for 20 weeks. Treatment with the PHAH mixtures caused liver enlargement and an increased activity of the hepatic cytochrome P4501A1/2 and P4502B1/2. All PHAH exposure groups exhibited an increased occurrence of hepatic foci positive for the placental form of glutathione-S-transferase. In the PHAH-group dosed 1 microgram TEQ/kg body wt/week, the volume fraction of the liver occupied by foci was significantly lower compared to the TEQ equivalent dosed TCDD group (3.8 vs 8.7%). The volume fraction was significantly increased in the groups treated with 0.5, 1, or 2 micrograms TEQ/kg body wt/week of the PHAH+ mixture (4.5, 5.2, and 6.6%, respectively) compared to the corn oil group (2.0%), but to a lower extent than expected on basis of the TEQ doses. Overall, the TEQ-based administered dose overestimated the observed tumor-promoting effects of this PHAH mixture. The applicability of the toxic equivalency factor concept, the role of differences in toxicokinetic properties and interactive effects of PCB 153 on hepatic deposition of the dioxin-like congeners are discussed.
The targets of violence committed by young offenders with alcohol dependence, marijuana dependence and schizophrenia-spectrum disorders: findings from a birth cohort.

BACKGROUND Estimates of who is most at risk from violence by people with mental illness rest mainly on identified patient samples. This study, without such selection bias, examined the targets of violence committed by young adults with as-yet untreated alcohol dependence, marijuana dependence, or schizophrenia-spectrum disorders, to determine the extent to which their victims were co-residents or non-household members. METHODS In a total birth cohort of 21-year-olds (n=956), past-year prevalence of alcohol dependence, marijuana dependence and schizophrenia-spectrum disorders were diagnosed using standardized DSM-III-R interviews. None of the people with schizophrenia-spectrum disorder has been hospitalized in the past year. Past-year violence and victim targets were measured using self-reports. RESULTS Compared with controls, cohort members with substance dependence or schizophrenia-spectrum disorders had higher prevalence and frequency rates of assault against co-residents, against non-household members, and also robbery and gang fights. Out of 39, five individuals with schizophrenia-spectrum disorder committed violent street crimes. Persons with substance dependence had similar proportions of violence against co-resident and non-household members, but persons with schizophrenia-spectrum disorders tended to victimize co-residents more than others. CONCLUSIONS At the age when they are most likely to contribute to the community's violence burden, young untreated offenders with alcohol or marijuana dependence or with schizophrenia-spectrum disorders assault not only co-residents, but others as well, and commit violent street crimes. Families, schoolteachers and primary care physicians have an important potentially preventive role in early identification and treatment of the disorders.
ODDSOC2 Is a MADS Box Floral Repressor That Is Down-Regulated by Vernalization in Temperate Cereals1[W][OA]

In temperate cereals, such as wheat (Triticum aestivum) and barley (Hordeum vulgare), the transition to reproductive development can be accelerated by prolonged exposure to cold (vernalization). We examined the role of the grass-specific MADS box gene ODDSOC2 (OS2) in the vernalization response in cereals. The barley OS2 gene (HvOS2) is expressed in leaves and shoot apices but is repressed by vernalization. Vernalization represses OS2 independently of VERNALIZATION1 (VRN1) in a VRN1 deletion mutant of einkorn wheat (Triticum monococcum), but VRN1 is required to maintain down-regulation of OS2 in vernalized plants. Furthermore, barleys that carry active alleles of the VRN1 gene (HvVRN1) have reduced expression of HvOS2, suggesting that HvVRN1 down-regulates HvOS2 during development. Overexpression of HvOS2 delayed flowering and reduced spike, stem, and leaf length in transgenic barley plants. Plants overexpressing HvOS2 showed reduced expression of barley homologs of the Arabidopsis (Arabidopsis thaliana) gene FLOWERING PROMOTING FACTOR1 (FPF1) and increased expression of RNase-S-like genes. FPF1 promotes floral development and enhances cell elongation, so down-regulation of FPF1-like genes might explain the phenotypes of HvOS2 overexpression lines. We present an extended model of the genetic pathways controlling vernalization-induced flowering in cereals, which describes the regulatory relationships between VRN1, OS2, and FPF1-like genes. Overall, these findings highlight differences and similarities between the vernalization responses of temperate cereals and the model plant Arabidopsis.
The effect of casinos on local labor markets: a county level analysis.

The economic outcomes surrounding the dramatic spread of “Las Vegas” style casinos in the United States has become a point of great interest and inquiry both politically and academically. Prior research has tended to focus on regional studies and provided uniform conclusions regardless of differences in the nature of the community. Moreover, much of the previous empirical work fails to account for local level trends during estimation. By using a comprehensive data set on employment and earnings from across the US, and by including county-specific trends, this research hopes to alleviate these earlier concerns, as well as help reconcile differences in the early literature surrounding casino effects on related industrial sectors. Basic findings suggest that counties experience an increase in employment after a casino opens, but there seems to be no measurable effect on average earnings. More detailed analysis reveals that the effect on industries related to casinos is somewhat mixed, but in general mildly positive, as casinos provide a positive employment and earnings spillovers into the surrounding local community. Intertemporal estimation suggests that the casino effect changes over time, but also finds that time effects vary across sectors. Estimates of how overall effects vary across different population sizes find that employment growth is inversely related to county population. Finally, additional estimation finds little impact on employment levels in neighboring counties, although there are some small effects in certain industries.
Randomized controlled trial of nutritional counseling on body composition and dietary intake in severe CKD.

BACKGROUND Progressive loss of kidney function results in an increased risk of malnutrition. Despite this, there is little evidence informing the impact of nutrition intervention on predialysis patients with chronic kidney disease (CKD; stages 4 and 5). STUDY DESIGN Randomized controlled trial. SETTING & PARTICIPANTS 56 outpatients (men, 62%; mean age, 70.7 +/- 14.0 [SD] years) with CKD were randomly allocated to intervention (n = 29) or control (n = 27) by using a concealed computer-generated sequence. INTERVENTION The intervention group, provided with individualized dietary counseling with regular follow-up aimed at achieving an intake of 0.8 to 1.0 g/kg of protein and greater than 125 kJ/kg of energy, or control, receiving written material only. OUTCOMES & MEASURES Change in body composition (body cell mass, measured by means of total-body potassium, in 40 of 56 participants), nutritional status (Subjective Global Assessment), and energy and protein intake (3-day food record). RESULTS During the 12 weeks, the intervention group had 3.5% (95% confidence interval, -2.1 to 9.1) less decrease in body cell mass, 17.7-kJ/kg/d (95% confidence interval, 8.2 to 27.2) greater increase in energy intake, greater improvement in Subjective Global Assessment (P < 0.01), and no significant difference in protein intake compared with the control group (-0.04 g/kg/d; 95% confidence interval, -0.73 to 0.16). The intervention was associated with greater increases in energy and protein intake in women than men (interaction P < 0.001 for both). LIMITATIONS Power to detect change in body cell mass, potential bias in ascertainment of Subjective Global Assessment. CONCLUSIONS In predialysis patients with CKD, structured nutrition intervention had a greater effect on energy and protein intake in women than men. Additional investigations are warranted to determine the impact on body composition.
Disparities in Surgical Care Among Women With Endometrial Cancer

OBJECTIVE: To analyze contemporary U.S. use of minimally invasive surgery for the treatment of endometrial cancer and associated inpatient complications and costs. METHODS: In this retrospective cohort study, the National Inpatient Sample database was analyzed in patients with nonmetastatic endometrial cancer who underwent hysterectomy during 2012–2013. Hierarchical multiple logistic regression and propensity score matching were used to compare complications among patients treated with open compared with minimally invasive hysterectomy surgery. Cost of care was also compared using generalized linear modeling. RESULTS: We identified 9,799 patients; 52.4% underwent open and 47.6% minimally invasive hysterectomy. Many patients (43.4%) were treated at low-volume hospitals (less than 10 endometrial cancer cases annually). Patients were less likely to undergo open surgery in high-volume compared with low-volume hospitals (51.8% compared with 58.1%, respectively; adjusted odds ratio [OR] 0.35, 95% confidence interval [CI] 0.13–0.94) and more likely to undergo open surgery in rural compared with urban teaching hospitals (75.6% compared with 51.1%, respectively; adjusted OR 14.34, 95% CI 9.66–21.27), government compared with nonprofit hospitals (61.3% compared with 51.1%, respectively; adjusted OR 1.66, 95% CI 1.15–2.39), and in patients of black (67.9%; OR 1.46, 95% CI 1.30–1.65) and “other” race (60.5%; adjusted OR 2.39, 95% CI 1.99–2.87) compared with white race (49.2%, referent). Open surgery was associated with increased perioperative complications (adjusted OR 2.80, 95% CI 2.48–3.17) and a $1,243 increase in cost per case compared with minimally invasive approaches (P<.001). Using minimally invasive surgery for 80% of study patients may have averted 2,733 complications and saved approximately $19 million. CONCLUSION: Most U.S. women with endometrial cancer continue to be treated with open hysterectomy surgery despite increased complication rates and financial costs associated with this approach. A disparity in endometrial cancer surgical care exists that is affected by patient race and hospital geography and cancer volumes.
Tubeless percutaneous nephrolithotomy: spinal versus general anesthesia.

PURPOSE Tubeless percutaneous nephrolithotomy (PCNL) with double-J stenting is a good option for large kidney calculi without increasing blood loss. In many centers tubeless PCNL is performed under general anesthesia. In the present study we evaluated the impact of spinal anesthesia in patients undergoing tubeless PCNL. MATERIAL AND METHODS Between February 2011 and February 2012, forty six patients with kidney calculi were treated with tubeless PCNL. Of these patients 26 were treated under spinal anesthesia (group 1) and remaining 20 were treated under general anesthesia (group 2). Groups were compared according to patient demographics, stone size, access number, operative time, presence of supracostal access, analgesic requirement, length of hospital stay, and complications. RESULTS There were not any statistically significant differences between groups in terms of patient demographics, mean stone size, mean access number, operative time, presence of supracostal access, and length of hospital stay. However, the analgesic requirement was significantly less in group 1 (53 ± 39 mg vs. 111 ± 46 mg, intravenous tramadol in groups 1 and 2, respectively P < .001). CONCLUSION Tubeless PCNL under spinal anesthesia is a good alternative for general anesthesia in adult patients. Spinal anesthesia decreases analgesic requirement in patients that were performed tubeless PCNL compared to general anesthesia.
Noether symmetries, energy–momentum tensors, and conformal invariance in classical field theory

In the framework of classical field theory, we first review the Noether theory of symmetries, with simple rederivations of its essential results, with special emphasis given to the Noether identities for gauge theories. With this baggage on board, we next discuss in detail, for Poincare invariant theories in flat spacetime, the differences between the Belinfante energy–momentum tensor and a family of Hilbert energy–momentum tensors. All these tensors coincide on shell but they split their duties in the following sense: Belinfante's tensor is the one to use in order to obtain the generators of Poincare symmetries and it is a basic ingredient of the generators of other eventual spacetime symmetries which may happen to exist. Instead, Hilbert tensors are the means to test whether a theory contains other spacetime symmetries beyond Poincare. We discuss at length the case of scale and conformal symmetry, of which we give some examples. We show, for Poincare invariant Lagrangians, that the realization of scale .
The impact of hiring top workers on productivity: what is the role of absorptive capacity?

ABSTRACT We examine the heterogeneous productivity impacts of hiring top workers on small and medium-sized enterprises, exploiting matched employer–employee panel data and employing within-firm as well as matching and difference-in-difference estimators. The results provide robust evidence that the productivity impact is stronger for firms with higher absorptive capacity. Technological laggards within an industry benefit more strongly from hiring top workers if their workforce is more well-educated.
Control mechanisms of gluconeogenesis and ketogenesis. I. Effects of oleate on gluconeogenesis in perfused rat liver.

Abstract The effects of oleate (1.0 to 1.5 mm) were studied in livers from fasted rats perfused with alanine (5 to 10 mm), l(+)-lactate (8 to 12 mm), pyruvate (1.5 to 2.0 mm), or dihydroxyacetone (10 mm) as gluconeogenic precursors. Rates of glucose production in control livers perfused with alanine, lactate, or pyruvate were 60, 120, and 95 µmoles per 100 g, body weight, per hour, respectively. Oleate increased these rates to 132, 250, and 194 µmoles per 100 g, body weight, per hour with the three substrates. With lactate as substrate, glucose formation accounted for 60% and 78% of the lactate uptake in the absence and presence of oleate. With pyruvate as substrate, about half of the pyruvate taken up by the liver was converted to glucose, both in the presence and absence of oleate. With dihydroxyacetone as substrate, the rate of glucose production was 250 µmoles per 100 g, body weight, per hour, and was not increased further by oleate. This indicates that the rate-controlling step of gluconeogenesis is prior to the formation of triose phosphates. Oleate increased the state of reduction of the pyridine nucleotide systems in both mitochondrial and cytosolic spaces as shown by increases in the ratios of lactate to pyruvate, β-hydroxybutyrate to acetoacetate, and malate to oxalacetate. Total tissue levels of the reduced forms of the pyridine nucleotides also increased after oleate administration. Measurements of metabolic intermediates of the gluconeogenic pathway were made in order to identify sites of interaction in the enzyme sequence. The significance of these interactions was analyzed by the crossover theorem. Glyceraldehyde-3-P dehydrogenase appeared as a control site only with alanine as substrate. It is proposed that this reaction was stimulated by the elevated NADH:NAD+ ratio maintained in the cytosol during enhanced fatty acid oxidation. With lactate and pyruvate as substrates, control sites were observed at pyruvate carboxylase (activation) and phosphofructokinase (inhibition). These effects were interpreted as being caused by elevated levels of acetyl coenzyme A and citrate. The interaction between fructose-1,6-di-P and fructose-6-P is interpreted as indicating recycling of these intermediates, which is at least as rapid as the increased glucose flux observed after oleate addition. This results in a wasteful use of ATP, but probably represents no more than 10 to 20% of the oxygen consumption. Control of pyruvate carboxylation is considered to represent the most physiologically meaningful interaction between fatty acid oxidation and gluconeogenesis.
Expression of CD28 on CD8+ and CD4+ Lymphocytes During HIV Infection

CD28 interaction with B7 molecules, expressed on the membranes of antigen‐presenting cells, costimulates cytokine production, T‐cell proliferation and generation of cytotoxic lymphocytes. The expression of CD28 markers on CD4+ and CD8+ lymphocytes was studied in a group of subjects at various stages of HIV infection. A reduction in the percentage of CD28‐bearing CD4+ and CD8+ cell subsets was observed during the asymptomatic stage of the disease. This reduction was more pronounced in AIDS than in non‐AIDS patients. At the same time, an increase in the absolute CD8+CD28− cell number (greater in stage A than in stage B and C subjects) was observed in HIV‐infected patients. The finding of an altered pattern of CD28 expression on T cells might per se explain certain early defects in the cytokine pattern and in the immune response peculiar to HIV‐infected patients.
Lentivector‐mediated delivery of GDNF protects complex motor functions relevant to human Parkinsonism in a rat lesion model

Although viral vector‐mediated delivery of glial cell‐line derived neurotrophic factor (GDNF) to the brain has considerable potential as a neuroprotective strategy in Parkinson's disease (PD), its ability to protect complex motor functions relevant to the human condition has yet to be established. In this study, we used an operant task that assesses the selection, initiation and execution of lateralized nose‐pokes in Lister Hooded rats to assess the efficacy with which complex behaviours are protected against neurotoxic lesions by prior injection of a lentiviral vector expressing GDNF. Unilateral injection of 6‐hydroxydopamine (6‐OHDA) into the medial forebrain bundle (MFB) caused rats to attempt fewer trials and to make more procedural errors. Lesioned rats also developed a pronounced ipsilateral bias, with a corresponding drop in contralateral accuracy. They were also slower to react to contralateral stimuli and to execute movements bilaterally. Rats that were pre‐treated 4 weeks prior to lesion surgery with an equine infectious anaemia virus (EIAV) vector carrying GDNF [EIAV‐GDNF, injected into the striatum and above the substantia nigra (SN)] performed significantly better on all of these parameters than control rats. In addition to the operant task, EIAV‐GDNF successfully rescued contralateral impairments in the corridor, staircase, stepping and cylinder tasks, and prevented drug‐induced rotational asymmetry. This study confirms that GDNF can protect against 6‐OHDA‐induced impairments in complex as well as simple behaviours, and reinforces the use of EIAV‐based vectors for the treatment of PD.
Larger-Context Language Modelling with Recurrent Neural Network

In this work, we propose a novel method to incorporate corpus-level discourse information into language modelling. We call this larger-context language model. We introduce a late fusion approach to a recurrent language model based on long short-term memory units (LSTM), which helps the LSTM unit keep intra-sentence dependencies and inter-sentence dependencies separate from each other. Through the evaluation on three corpora (IMDB, BBC, and PennTree Bank), we demon- strate that the proposed model improves perplexity significantly. In the experi- ments, we evaluate the proposed approach while varying the number of context sentences and observe that the proposed late fusion is superior to the usual way of incorporating additional inputs to the LSTM. By analyzing the trained larger- context language model, we discover that content words, including nouns, adjec- tives and verbs, benefit most from an increasing number of context sentences. This analysis suggests that larger-context language model improves the unconditional language model by capturing the theme of a document better and more easily.