Miracle-dz/UniFAR-FTUs huggingface dataset:
Proprotein Convertase Subtilisin/Kexin Type 7 (PCSK7) Is Essential for the Zebrafish Development and Bioavailability of Transforming Growth Factor β1a (TGFβ1a)*

Background: The in vivo importance of PCSK7 in the vertebrates is currently poorly understood. Results: Inhibiting PCSK7 in zebrafish results in various developmental defects and dysregulation of gene expressions. Conclusion: PCSK7 is essential for zebrafish development and regulates the expression and proteolytic cleavage of TGFβ1a. Significance: PCSK inhibitors are considered future therapeutics for human diseases; understanding the biological role of PCSK7 is therefore critical. Proprotein convertase subtilisin/kexin (PCSK) enzymes convert proproteins into bioactive end products. Although other PCSK enzymes are known to be essential for biological processes ranging from cholesterol metabolism to host defense, the in vivo importance of the evolutionarily ancient PCSK7 has remained enigmatic. Here, we quantified the expressions of all pcsk genes during the 1st week of fish development and in several tissues. pcsk7 expression was ubiquitous and evident already during the early development. To compare mammalian and zebrafish PCSK7, we prepared homology models, which demonstrated remarkable structural conservation. When the PCSK7 function in developing larvae was inhibited, we found that PCSK7-deficient fish have defects in various organs, including the brain, eye, and otic vesicle, and these result in mortality within 7 days postfertilization. A genome-wide analysis of PCSK7-dependent gene expression showed that, in addition to developmental processes, several immune system-related pathways are also regulated by PCSK7. Specifically, the PCSK7 contributed to the mRNA expression and proteolytic cleavage of the cytokine TGFβ1a. Consequently, tgfβ1a morphant fish displayed phenotypical similarities with pcsk7 morphants, underscoring the importance of this cytokine in the zebrafish development. Targeting PCSK activity has emerged as a strategy for treating human diseases. Our results suggest that inhibiting PCSK7 might interfere with normal vertebrate development.
Semaphorin 3a transfection into the left stellate ganglion reduces susceptibility to ventricular arrhythmias after myocardial infarction in rats.

AIMS Myocardial infarction (MI) induces neural remodelling of the left stellate ganglion (LSG), which may contribute to ischaemia-induced arrhythmias. The neural chemorepellent Semaphorin 3a (Sema3a) has been identified as a negative regulator of sympathetic innervation in the LSG and heart. We previously reported that overexpression of Sema3a in the border zone could reduce the arrhythmogenic effects of cardiac sympathetic hyperinnervation post-MI. This study investigated whether Sema3a overexpression within the LSG confers an antiarrhythmic effect after MI through decreasing extra- and intra-cardiac neural remodelling. METHODS AND RESULTS Sprague-Dawley rats were subjected to MI, and randomly allocated to intra-LSG microinjection of either phosphate-buffered saline (PBS), adenovirus encoding green fluorescent protein (AdGFP), or adenovirus encoding Sema3a (AdSema3a). Sham-operated rats served as controls. Two weeks after infarction, MI-induced nerve sprouting and sympathetic hyperinnervation in the LSG and myocardium were significantly attenuated by intra-LSG injection with AdSema3a, as assessed by immunohistochemistry and western blot analysis of growth-associated protein 43 and tyrosine hydroxylase. This was also confirmed by sympathetic nerve function changes assessed by cardiac norepinephrine content. Additionally, intra-LSG injection with AdSema3a alleviated MI-induced accumulation of dephosphorylated connexin 43 in the infarct border zone. Furthermore, Sema3a overexpression in the LSG reduced the incidence of inducible ventricular tachyarrhythmia by programmed electrical stimulation post-MI, and arrhythmia scores were significantly lower in the AdSema3a group than in the PBS and AdGFP groups. CONCLUSION Semaphorin 3a overexpression in the LSG ameliorates the inducibility of ventricular arrhythmias after MI, mainly through attenuation of neural remodelling within the cardiac-neuraxis.
Carbon monoxide overproduced by heme oxygenase-1 causes a reduction of vascular resistance in perfused rat liver

YOSHIYUKI WAKABAYASHI,1 RINA TAKAMIYA,1 AKIRA MIZUKI,2 TAKANORI KYOKANE,1 NOBUHITO GODA,1 TOKIO YAMAGUCHI,3 SHINJI TAKEOKA,4 EISHUN TSUCHIDA,4 MAKOTO SUEMATSU,1 AND YUZURU ISHIMURA1 1Department of Biochemistry, School of Medicine, Keio University, 2Department of Medicine, Saisei-kai Central Hospital, 4Department of Polymer Chemistry, Waseda University, and 3Department of Biochemical Genetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 160, Japan
Fertility and competitiveness of Culex quinquefasciatus males irradiated in nitrogen.

Male sterility and sexual competitiveness were evaluated after irradiation (Cesium-137 source) of 20- to 26-h-old pupae and 0- to 20-h-old adults of Culex quinquefasciatus Say in air and nitrogen (N2. The presence of nitrogen protected both stages against the induction of posttreatment sterility. In nitrogen, the exposure required for 98% sterility had to be increased from 8 kR in air to 20 kR. At lower irradiation dosages, nitrogen offered more protection of genetic tissues for pupae than adults. However, no improvement was observed in the mating competitiveness when air was replaced with nitrogen during pupal irradiation, and only marginal improvement was observed after adult exposure. Because of its deleterious effect on male competitiveness, irradiation may have limited usefulness as a method of sterilization for this species.
Effects of ethanol on kinaesthetic perception

In normal subjects, alcohol increases handwriting size, but the mechanism is not understood. Here we show that the alcohol effect on handwriting can be explained by a selective impairment of kinaesthetic perception. Thirty volunteers (15 male, aged 18-29 years) took part in an open study. They were tested before and after a drink containing vodka intended to produce a blood alcohol concentration of about 80mg/100ml. Tests included kinaesthetic distance estimation, in which volunteers worked with preferred hand and arm behind a screen which hid their movements; visual distance estimation; and measures of handwriting and drawing. Blood alcohol concentration at 55min, based on breathalyser measurements, was 76.7mg/100ml (SD 9.8). When asked to move the hand and mark a distance of 10cm from a starting point, distances estimates increased by 7-10% (p 0.01). Similar increases were seen for writing words and drawing characters. Signatures were increased in height but not in length. Distances estimated visually were increased much less, by 3-4% (p 0.05). Tests of psychomotor performance indicated the expected effects of ethanol. These results suggest that ethanol affects writing size by reducing kinaesthetically perceived distances.
Discrimination of alcoholic from virus-induced cirrhosis on MR imaging.

OBJECTIVE The purpose of this study was to determine whether MR features help to differentiate virus-induced cirrhosis from alcoholic cirrhosis. MATERIALS AND METHODS The MR examinations of 53 patients with cirrhosis (38 men and 15 women; age range, 28-73 years) caused by hepatitis B (n = 16), hepatitis C (n = 18), or alcohol abuse (n = 19) were retrospectively reviewed independently by two radiologists. The following MR features were assessed by each radiologist independently: volume indexes of the spleen and of each liver segment (based on 3-axis measurements), the nodularity of the surface, and the presence of regenerative nodules, ascites, iron or fat deposition, varices or collateral veins, the right posterior hepatic notch, and an expanded gallbladder fossa. RESULTS The mean values of the volume index of the caudate lobe were significantly greater (p < 0.0001) in the group with alcoholic cirrhosis than those in the group with viral cirrhosis. The frequency of visualization of the right posterior hepatic notch in the patients with alcoholic cirrhosis was significantly greater (p < 0.05) than that in the patients with viral cirrhosis. The size of regenerative nodules of the liver in the patients with cirrhosis caused by hepatitis B was significantly greater (p < 0.02) than that in the patients with alcoholic cirrhosis. CONCLUSION Enlargement of the caudate lobe and the presence of the right posterior hepatic notch on MR imaging are more frequent findings of alcoholic cirrhosis than of virus-induced cirrhosis.
Enhancement on Wingate Anaerobic Test Performance With Hyperventilation.

UNLABELLED Relatively long-lasting metabolic alkalizing procedures such as bicarbonate ingestion have potential for improving performance in long-sprint to middle-distance events. Within a few minutes, hyperventilation can induce respiratory alkalosis. However, corresponding performance effects are missing or equivocal at best. PURPOSE To test a potential performance-enhancing effect of respiratory alkalosis in a 30-s Wingate Anaerobic Test (WAnT). METHODS 10 men (mean ± SD age 26.6 ± 4.9 y, height 184.4 ± 6.1 cm, body-mass test 1 80.7 ± 7.7 kg, body-mass test 2 80.4 ± 7.2 kg, peak oxygen uptake 3.95 ± 0.43 L/min) performed 2 WAnTs, 1 with and 1 without a standardized 15-min hyperventilation program pre-WAnT in randomized order separated by 1 wk. RESULTS Compared with the control condition, hyperventilation reduced (all P < .01) pCO2 (40.5 ± 2.8 vs 22.5 ± 1.6 mm Hg) and HCO3 - (25.5 ± 1.7 vs 22.7 ± 1.6 mmol/L) and increased (all P < .01) pH (7.41 ± 0.01 vs 7.61 ± 0.03) and actual base excess (1.4 ± 1.4 vs 3.2 ± 1.6 mmol/L) pre-WAnT with an ergogenic effect on WAnT average power (681 ± 41 vs 714 ± 44 W) and total metabolic energy (138 ± 12 vs. 144 ± 13 kJ) based on an increase in glycolytic energy (81 ± 13 vs 88 ± 13 kJ). CONCLUSION Hyperventilation-induced respiratory alkalosis can enhance WAnT cycling sprint performance well in the magnitude of what is seen after successful bicarbonate ingestion.
The red-edge effects: 30 years of exploration.

In 1970, three laboratories independently made a discovery that, for aromatic fluorophores embedded into different rigid and highly viscous media, the spectroscopic properties do not conform to classical rules. The fluorescence spectra can depend on excitation wavelength, and the excited-state energy transfer, if present, fails at the "red" excitation edge. These red-edge effects were related to the existence of excited-state distribution of fluorophores on their interaction energy with the environment and the slow rate of dielectric relaxation of this environment. In these conditions the site-selection can be provided by variation of the energy of illuminating light quanta, and the behaviour of selected species can be followed as a function of time and other variables. These observations found extensive application in different areas of research: colloid and polymer science, molecular biophysics, photochemistry and photobiology. In particular, they led to the development of very productive methods of studying the dynamics of dielectric relaxations in protein and membranes, using the tryptophan emission and the emission of a variety of probes. These studies were extended to the time domain with the observation of new site-selective effects in emission intensity and anisotropy decays. They stimulated the emergence and development of cryogenic energy-selective and single-molecular techniques that became valuable tools in their own right in chemistry and biophysics research. Site-selection effects were discovered for electron-transfer and proton-transfer reactions if they depended on the dynamics of the environment. This review is focused on the progress in the field of red-edge effects, their applications and prospects.
Effect of Electron Donor and Solution Chemistry on Products of Dissimilatory Reduction of Technetium byShewanella putrefaciens

ABSTRACT To help provide a fundamental basis for use of microbial dissimilatory reduction processes in separating or immobilizing99Tc in waste or groundwaters, the effects of electron donor and the presence of the bicarbonate ion on the rate and extent of pertechnetate ion [Tc(VII)O4−] enzymatic reduction by the subsurface metal-reducing bacterium Shewanella putrefaciens CN32 were determined, and the forms of aqueous and solid-phase reduction products were evaluated through a combination of high-resolution transmission electron microscopy, X-ray absorption spectroscopy, and thermodynamic calculations. When H2served as the electron donor, dissolved Tc(VII) was rapidly reduced to amorphous Tc(IV) hydrous oxide, which was largely associated with the cell in unbuffered 0.85% NaCl and with extracellular particulates (0.2 to 0.001 μm) in bicarbonate buffer. Cell-associated Tc was present principally in the periplasm and outside the outer membrane. The reduction rate was much lower when lactate was the electron donor, with extracellular Tc(IV) hydrous oxide the dominant solid-phase reduction product, but in bicarbonate systems much less Tc(IV) was associated directly with the cell and solid-phase Tc(IV) carbonate may have been present. In the presence of carbonate, soluble (<0.001 μm) electronegative, Tc(IV) carbonate complexes were also formed that exceeded Tc(VII)O4− in electrophoretic mobility. Thermodynamic calculations indicate that the dominant reduced Tc species identified in the experiments would be stable over a range of Eh and pH conditions typical of natural waters. Thus, carbonate complexes may represent an important pathway for Tc transport in anaerobic subsurface environments, where it has generally been assumed that Tc mobility is controlled by low-solubility Tc(IV) hydrous oxide and adsorptive, aqueous Tc(IV) hydrolysis products.
The 6‐49 Lottery Paradox

The paradox of the preface and the lottery paradox are paradoxes of practical certainty sharing certain features. The paradox of the lottery argues that rational agents are at once practically certain that each ticket in a lottery will lose but also practically certain some ticket will win. The paradox of the preface argues that rational agents are at once practically certain that all facts in a written volume are true, yet are also practically certain that some fact is wrong. A difference between real lotteries and prefaces is that a winning lottery ticket is generally an intended feature of the lottery, whereas incorrect facts are generally unintended.
Role of N‐cadherin in the sorting‐out of mesenchymal cells and in the positional identity along the proximodistal axis of the chick limb bud

Mesenchymal cells from different stages of chick limb buds sort out in monolayer culture, suggesting the presence of different cell affinities dependent on their positions along the proximodistal axis. However, it is still not clear which molecules are responsible for the sorting‐out. Here, we propose that N‐cadherin, a cell‐adhesion molecule, is involved in the sorting‐out and is likely to be a component of the mechanism of proximodistal patterning in the developing limb. N‐cadherin proteins accumulate in the distal region of the chick limb bud as limb development proceeds. In monolayer culture of distal mesenchymal cells, the stage‐dependent levels of N‐cadherin proteins are maintained during cell sorting. The results of this study have also demonstrated that an anti‐N‐cadherin monoclonal antibody, NCD‐2, clearly inhibits the cell sorting. Moreover, removal of the apical ectodermal ridge or retinoic‐acid treatment of distal cells, which results in a change in the pattern of sorting‐out, inhibits the accumulation of N‐cadherin proteins, suggesting that the distribution of these proteins is related to the positional identity that gives rise to the different shape and number of cartilage elements along the proximodistal axis. Dev Dyn 1999;216:274–284. ©1999 Wiley‐Liss, Inc.
Outcome of Percutaneous Surgery Stratified According to Body Mass Index and Kidney Stone Size

Objective To stratify outcome and morbidity of percutaneous nephrostolithotomy (PCNL) with regard to body mass index (BMI) and kidney stone burden. Methods The charts of 148 patients who underwent PCNL procedures were reviewed retrospectively. Hospital stay, blood loss, maximal temperature during inpatient stay, and stone-free outcomes were evaluated. Patients were divided into 3 groups depending on their BMIs: <25 kg/m2, 25 to 29.9 kg/m2, and >30 kg/m2. Kidney stone burden was measured in terms of square area in millimeters, as measured by retrospective review of computerized tomography scans. Preoperative computerized tomography scan for measurement of stone burden was available for only 85 patients who are included in the study. Analysis of variance for a single variable was performed with regard to the values of the hospital stay, postoperative maximal temperature, and hemoglobin change. Results Of the 85 patients, 37 (43.5%) were obese or morbidly obese (BMI, >30 kg/m2), 33 (38.8%) were overweight (BMI, 25 to 29.9 kg/m2), and 15 (17.7%) were within or below their ideal weight (BMI, <25 kg/m2). No statistically significant difference among the 3 groups was seen for stone-free rate, postoperative fever, or change in hemoglobin when stratified by BMI alone or by BMI and kidney stone burden. However, significantly longer length of stay for the group with BMI <25 kg/m2 was observed when stratifying either by BMI alone (P=0.01) or by BMI and kidney stone burden (P=0.03). Conclusions In this retrospective review of patients with kidney stones undergoing PCNL, the stone-free outcome and associated morbidity of PCNL (except for the length of hospital stay) is independent of both patients' BMI and stone burden when stratifying by commonly defined parameters.
Cholecystokinin in Mammalian Primary Sensory Neurons and Spinal Cord: In Situ Hybridization Studies in Rat and Monkey

The peptide cholecystokinin (CCK) has been suggested to be involved in nociception, but its exact localization at the level of the spinal cord and in spinal ganglia has been a controversial issue. Therefore the distribution of messenger RNA (mRNA) for CCK was studied by in situ hybridization using oligonucleotide probes on sections of adult rat lumbar dorsal root ganglia following unilateral section of the sciatic nerve and on sections of untreated monkey trigeminal ganglia, spinal cord and spinal ganglia from all levels. For comparison, calcitonin gene‐related peptide (CGRP) mRNA was also studied in the monkey tissue using the same techniques. Peripheral sectioning of the sciatic nerve in the rat resulted in the appearance of detectable CCK mRNA in up to 30% of remaining ipsilateral L4 and L5 dorsal root ganglion neurons 3 weeks after surgery, with a distinct but more limited appearance also in the contralateral ganglia. No cells, or only single cells, could be seen in normal control rat ganglia. In contrast, in the normal monkey, ∼20% of dorsal root ganglion neurons, regardless of spinal level, and 10% of trigeminal ganglia neurons expressed mRNA for CCK. CGRP mRNA was expressed at detectable levels in ∼80% of these monkey dorsal root ganglion neurons. In the monkey spinal cord, CCK mRNA was detected in the dorsal horn and in motoneurons, whereas CGRP mRNA was only seen in motoneurons. The present results suggest that CCK peptides can be involved in sensory processing in the dorsal horn of the spinal cord in normal monkeys and in rats after peripheral nerve injury, adding one more possible excitatory peptide to the group of mediators in the dorsal horn.
A rule based taxonomy of dirty data.

There is a growing awareness that high quality of data is a key to today’s business success and that dirty data existing within data sources is one of the causes of poor data quality. To ensure high quality data, enterprises need to have a process, methodologies and resources to monitor, analyze and maintain the quality of data. Nevertheless, research shows that many enterprises do not pay adequate attention to the existence of dirty data and have not applied useful methodologies to ensure high quality data for their applications. One of the reasons is a lack of appreciation of the types and extent of dirty data. In practice, detecting and cleaning all the dirty data that exists in all data sources is quite expensive and unrealistic. The cost of cleaning dirty data needs to be considered for most of enterprises. This problem has not attracted enough attention from researchers. In this paper, a rule-based taxonomy of dirty data is developed. The proposed taxonomy not only provides a mechanism to deal with this problem but also includes more dirty data types than any of existing such taxonomies.
FLOCK-BASED SURVEILLANCE FOR LOW PATHOGENIC AVIAN INFLUENZA VIRUS IN COMMERCIAL BREEDERS AND LAYERS, SOUTHWEST NIGERIA.

BACKGROUND Flock surveillance systems for avian influenza (AI) virus play a critical role in countries where vaccination is not practiced so as to establish the epidemiological characteristics of AI needed for the development of prevention and control strategies in such countries. MATERIALS AND METHODS As part of routine AI monitoring in southwest Nigeria, a competitive ELISA was used for detecting influenza A virus antibodies in the sera of 461 commercial breeder and layer birds obtained from different flocks in Oyo State, Nigeria while haemagglutination inhibiting antibodies against low pathogenic AI viruses (LPAIVs) were detected using H5N2, H7N7 and H9N2 subtype-specific antigens. Suspensions prepared from cloacal swabs were tested for AI virus RNA using reverse transcriptase-polymerase chain reaction. RESULTS Results showed that influenza A virus antibody prevalence was 12.8% and 9.3% for breeders and layers, respectively while HI assay revealed 22.0%, 2.0% and 78.0% prevalence of LPAIV H5N2, H7N7 and H9N2 antibodies respectively. All cloacal swab suspensions were negative for AIV RNA. CONCLUSION Since LPAI infections result in decreased or complete cessation of egg production in breeder and layer birds, increased infection severity due to co-infection with other poultry viruses have occasionally been transmitted to humans, the detection of LPAIV H5N2, H7N7 and H9N2 antibodies in these birds is of both economic and public health significance. These findings underscore the need for continuous flock monitoring as part of early warning measure to facilitate rapid detection and sustainable control of AI in Nigerian poultry.
Ocular Safety of Intravitreal Propranolol and Its Efficacy in Attenuation of Choroidal Neovascularization.

PURPOSE Determine the safe dose of intravitreal propranolol (IVP), and evaluate its inhibitory effect on laser-induced choroidal neovascularization (CNV). METHODS To determine the IVP safe dose, 32 rabbits were divided into 4 groups. Three of these groups received IVP (15 μL) corresponding to 15 μg (group B), 30 μg (group C), and 60 μg (group D). The control group (A) received 15 μL saline. Safety was assessed by ocular examination, electroretinography (ERG), routine histopathologic evaluation, immunohistochemistry for glial fibrillary acidic protein (GFAP), and real-time qPCR for GFAP, VEGF, thrombospondin 1 (TSP1), and pigment epithelium-derived factor (PEDF). A similar experiment was performed in 24 mice by using a 100-fold lower amount of propranolol (0.15, 0.3, and 0.6 μg in 2 μL) based on vitreous volume. For assessment of the angioinhibitory effects of IVP, CNV was induced in 42 mice via laser burns. Mice were divided into two groups: group 1 received the safe dose of IVP (0.3 μg in 2 μL) and group 2 received saline. Neovascularization area was quantified by intercellular adhesion molecule (ICAM)-2 immunostaining of choroidal-scleral flat mounts by using ImageJ software. RESULTS According to clinical, ERG, and histopathologic findings, 30 μg IVP was chosen as the safe dose in rabbit eyes, comparable to 0.3 μg IVP in mouse eyes. As compared to the control eyes, the development of CNV was attenuated (4.8-fold) in mice receiving 0.3 μg IVP. CONCLUSIONS Intravitreal propranolol injection up to the final dose of 30 μg in rabbits and 0.3 μg in mice was safe, and was effective in attenuation of CNV in mice.