Miracle-dz/UniFAR-FTUs huggingface dataset:
Pathophysiology of Nonalcoholic Steatohepatitis

Nonalcoholic steatohepatitis (NASH), occurs frequently with diabetes mellitus and obesity, contributes to insulin resistance, can develop cirrhosis or even hepatocellular carcinoma (HCC). Although NASH has become more common, its underlying mechanism is still not clear and effective therapy has not been established. Currently, it has been recognized that the multiple parallel factors, including genetic differences, insulin resistance, lipotoxicity and dysbiosis act synergistically in genetically in pathogenesis of NASH. Impaired autophagy parallel to excess lipid supply might explain the progression of NASH to HCC. Liver-specific knockout mice of phosphatase and tensin homolog deleted on chromosome ten (PTEN) develops steatohepatitis followed by HCC, suggesting a potential role for this molecule in development and progression of NASH.
Gamma Interferon Is Not Required for Mucosal Cytotoxic T-Lymphocyte Responses or Heterosubtypic Immunity to Influenza A Virus Infection in Mice

ABSTRACT Heterosubtypic immunity (HSI) is defined as cross-protection against influenza virus of a different serotype than the virus initially encountered and is thought to be mediated by influenza virus-specific cytotoxic T lymphocytes (CTL). Since gamma interferon (IFN-γ) stimulates cytotoxic cells, including antigen-specific CTL which may control virus replication by secretion of antiviral cytokines such as tumor necrosis factor alpha and IFN-γ, we have investigated the mechanism of HSI by analyzing the role of IFN-γ for HSI in IFN-γ gene-deleted (IFN-γ−/−) mice. It has been reported that IFN-γ is not required for recovery from primary infection with influenza virus but is important for HSI. Here, we conclusively show that IFN-γ is not required for induction of secondary influenza virus-specific CTL responses in mediastinal lymph nodes and HSI to lethal influenza A virus infection. Although T helper 2 (Th2)-type cytokines were upregulated in the lungs of IFN-γ−/− mice after virus challenge, either Th1- or Th2-biased responses could provide heterosubtypic protection. Furthermore, titers of serum-neutralizing and cross-reactive antibodies to conserved nucleoprotein in IFN-γ−/− mice did not differ significantly from those in immunocompetent mice. These results indicate that lack of IFN-γ does not impair cross-reactive virus-specific immune responses and HSI to lethal infection with influenza virus. Our findings provide new insight for the mechanisms of HSI and should be valuable in the development of protective mucosal vaccines against variant virus strains, such as influenza and human immunodeficiency virus.
Multiagent based particle swarm optimization approach to economic dispatch with security constraints

This paper proposes a new evolutionary approach named as multi agent particle swarm optimization (MAPSO) algorithm for solving economic dispatch with security constraints (line flow and bus voltage). This method integrates multiagent systems (MAS) and particle swarm optimization (PSO) to form a new algorithm, multiagent particle swarm optimization algorithm. In MAPSO, an agent represents a particle to PSO and a candidate solution to the optimization problem. All agents live in a lattice like environment, with each agent fixed on a lattice point. In order to obtain optimal solution, each agent competes and cooperates with its neighbor and it can also learn by using its knowledge. Making use of these agent-agent interactions, MAPSO realizes the purpose of minimizing the objective function value. MAPSO is applied to two representative systems i.e. IEEE 14 bus and IEEE 30 bus systems. Simulation results show that proposed approach gives better solution than earlier reported approaches.
Contacts de langues en Guyane française : une description du parler bilingue kali'na-français

Cette these presente les resultats d'une recherche menee en Guyane francaise aupres d'un groupe d'enfants d'une classe de CM2 de l'ecole d'Awala-Yalimapo. Ces enfants appartiennent a l'une des six communautes amerindiennes du departement et ont pour langue premiere le kali'na, langue de la famille caribe. La recherche a consiste en une description et une analyse des productions des enfants dans deux situations de communication, toutes deux situees dans l'ecole : la salle de classe et la cour de recreation. L'objectif etant de mettre en evidence les processus linguistiques intervenant dans la communication entre bilingues etant entendu une situation sociolinguistique donnee. Cette etude s'inscrit pleinement dans les debats suscites dans la recherche actuelle par la question du contact des langues et du bilinguisme et, par extension, dans les tentatives visant a etablir une taxinomie des processus en œuvre dans une situation de contact des langues. L'approche preconisee est l'approche dite " bilinguiste "selon laquelle le contact des langues a lieu en premiere instance chez l'individu bilingue ou, plus precisement, dans les interactions comportant au moins un bilingue. L'hypothese de depart est donc que le changement linguistique se construit dans les micro-evenements langagiers et que les interactions sont les expressions premieres des situations de contact. Une telle approche permet de mettre en evidence les observables manifestant la presence de plusieurs langues dans les productions interlinguales tels que les emprunts ou les alternances et melanges codiques. Ces processus sont decrits et analyses tout au long de cette etude, ils sont mis en relation avec diverses theories relevant du domaine du contact des langues et du bilinguisme. Ce memoire presente en conclusion une approche synthetique, a la fois macro- et micro-sociolinguistique permettant de classer le parler bilingue kali'na-francais dans une typologie du discours bilingue selon que ce discours se situe dans la salle de classe, ou les eleves se comportent plus comme des apprenants, ou qu'il se situe dans la cours de recreation, ou ils se comportent comme des bilingues.
Temporal Delay of Peak T-Cell Immunity Determines Chlamydia pneumoniae Pulmonary Disease in Mice

ABSTRACT Severe chlamydial disease typically occurs after previous infections and results from a hypersensitivity response that is also required for chlamydial elimination. Here, we quantitatively dissected the immune and disease responses to repeated Chlamydia pneumoniae lung infection by multivariate modeling with four dichotomous effects: mouse strain (A/J or C57BL/6), dietary protein content (14% protein and 0.3% l-cysteine-0.9% l-arginine, or 24% protein and 0.5% l-cysteine-2.0% l-arginine), dietary antioxidant content (90 IU α-tocopherol/kg body weight versus 450 IU α-tocopherol/kg and 0.1% g l-ascorbate), and time course (3 or 10 days postinfection). Following intranasal C. pneumoniae challenge, C57BL/6 mice on a low-protein/low-antioxidant diet, but not C57BL/6 mice on other diets or A/J mice, exhibited profoundly suppressed early lung inflammatory and pan-T-cell (CD3δ+) and helper T-cell (CD45) responses on day 3 but later strongly exacerbated disease on day 10. Contrast analyses characterized severe C. pneumoniae disease as being a delayed-type hypersensitivity (DTH) response with increased lung macrophage and Th1 cell marker transcripts, increased Th1:Th2 ratios, and Th1 cytokine-driven inflammation. Results from functional analyses by DTH, enzyme-linked immunospot, and immunohistofluorescence assays were consistent with the results obtained by transcript analysis. Thus, chlamydial disease after secondary infection is a temporal dysregulation of the T-cell response characterized by a profoundly delayed T-helper cell response that results in a failure to eliminate the pathogen and provokes later pathological Th1 inflammation. This delayed T-cell response is under host genetic control and nutritional influence. The mechanism that temporally and quantitatively regulates the host T-cell population is the critical determinant in chlamydial pathogenesis.
Little Association Between Wellness Policies and School-Reported Nutrition Practices

Background. The Child Nutrition and WIC Reauthorization Act of 2004 mandated written school wellness policies. Little evidence exists to evaluate the impact of such policies. This study assessed the quality (comprehensiveness of topics addressed and strength of wording) of wellness policies and the agreement between written district-level policies and school-reported nutrition policies and practices in 48 low-income Michigan school districts participating in the School Nutrition Advances Kids study. Method. Written wellness policy quality was assessed using the School Wellness Policy Evaluation Tool. School nutrition policies and practices were assessed using the School Environment and Policy Survey. Analysis of variance determined differences in policy quality, and Fisher’s exact test examined agreement between written policies and school-reported practices. Results. Written wellness policies contained ambiguous language and addressed few practices, indicating low comprehensiveness and strength. Most districts adopted model wellness policy templates without modification, and the template used was the primary determinant of policy quality. Written wellness policies often did not reflect school-reported nutrition policies and practices. Conclusions. School health advocates should avoid assumptions that written wellness policies accurately reflect school practices. Encouraging policy template customization and stronger, more specific language may enhance wellness policy quality, ensure consistency between policy and practice, and enhance implementation of school nutrition initiatives.
The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex.

Many mammalian receptors have been found to regulate cell growth by virtue of a protein-tyrosine kinase domain in their cytoplasmic tail. We recently described an association of the CD4 antigen with a T-cell-specific protein-tyrosine kinase (p56lck; formerly termed pp58lck; EC 2.7.1.112). This interaction represents a potential mechanism by which T-cell growth may be regulated and offers a model by which other members of the src family (products of c-src, c-yes, c-fgr, etc.) may interact with mammalian growth factor receptors. As in the case of the CD4 antigen, the CD8 antigen appears to serve as a receptor for nonpolymorphic regions of products of the major histocompatibility complex and has been implicated in the regulation of T-cell growth. In this study, we reveal that the human CD8 antigen is also associated with the T-cell-specific protein-tyrosine kinase (p56lck). The associated p56lck kinase was detected by use of both in vitro and in vivo labeling regimes using an antiserum to the C terminus of p56lck. Two-dimensional nonequilibrium pH-gradient gel electrophoresis and sodium dodecyl sulfate/polyacrylamide gel electrophoresis demonstrated the similarity of p56lck to the protein-tyrosine kinase associated with the CD4 antigen. The catalytic activity of p56lck was revealed by the autophosphorylation of the 55- to 60-kDa kinase and the occasional labeling of a 35-kDa protein. Last, we demonstrate directly that members of the CD3 complex, including the gamma, delta, and epsilon chains, as well as a putative zeta subunit, can be phosphorylated at tyrosine residues by the CD4/CD8.p56lck complex.
Development of a residency interviewing preparatory seminar.

PURPOSE The development of a residency interviewing preparatory seminar (RIPS) is described. SUMMARY The RIPS elective at Nova Southeastern University College of Pharmacy was designed to assist pharmacy students during their last professional year in preparing for the residency application process. The learning objectives of the course focused on improving students' interviewing and presentation skills, professionalism, and developing their curriculum vitae (CV) and personal statement. Course and instructor evaluations and demographic data collected via anonymous surveys were used to ascertain students' perspectives about the course. The class was purposely scheduled to begin in October and end in November, right before the ASHP Midyear Clinical Meeting in early December. Due to limited faculty availability on the scheduled evenings and the desire to provide tailored, in-depth feedback, enrollment was limited. The RIPS was an intensive eight-week elective completed by 10 fourth-year pharmacy students. The course began with an overview of the residency application process, and students submitted their CVs and personal statements to faculty mentors for critique. To simulate residency interview components, students completed several timed activities in class and participated in mock interviews. Students stated that the course improved their application materials, interview skills, and confidence in their ability to obtain a residency. Overall, 78% of RIPS participants matched with a residency program. CONCLUSION The RIPS elective was successful in improving residency candidate confidence at the ASHP Midyear Clinical Meeting. Students reported that the course was helpful and improved their confidence and ability to interview.
Treatment of the Elderly with 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Inhibitors: Focus on Drug Interactions

With the aging of the population, death from coronary heart disease (CHD) and stroke has become more prevalent. Cardiovascular disease (CVD) risk factors, such as hypertension, obesity, and diabetes mellitus increase with age as well. Recent secondary-prevention studies have established the positive effect of statins in decreasing the risk of CHD mortality through the lowering of cholesterol. Statins have an excellent safety record, at least with users under age 65, and provide a cheaper alternative to more costly medical options. The most serious side effect associated with their use is myopathy, which is infrequent. Drug interactions have been found with drugs that compete for the same CYP450 isoenzymes as statins. Several drugs have been shown to significantly inhibit the CYP3A4 pathway; in combination with statins such as lovastatin, simvastatin, atorvastatin, and cerivastatin, they have been shown to elevate serum concentrations of these statins, or may increase the risk of myopathy. Alternatively, other drugs can inhibit the CYP2C9 pathway and may elevate serum concentration of fluvastatin. Due to the number of medications the elderly receive, an understanding of the various metabolic pathways is of vital importance to minimize the potential for drug interactions. The elderly population, while at high risk for CVD, is currently undertreated. Statins can effectively lower low-density lipoprotein cholesterol levels and lessen the risk of CVD for this population.
Working Memory, Age, and Hearing Loss: Susceptibility to Hearing Aid Distortion

Objectives: Hearing aids use complex processing intended to improve speech recognition. Although many listeners benefit from such processing, it can also introduce distortion that offsets or cancels intended benefits for some individuals. The purpose of the present study was to determine the effects of cognitive ability (working memory) on individual listeners’ responses to distortion caused by frequency compression applied to noisy speech. Design: The present study analyzed a large data set of intelligibility scores for frequency-compressed speech presented in quiet and at a range of signal-to-babble ratios. The intelligibility data set was based on scores from 26 adults with hearing loss with ages ranging from 62 to 92 years. The listeners were grouped based on working memory ability. The amount of signal modification (distortion) caused by frequency compression and noise was measured using a sound quality metric. Analysis of variance and hierarchical linear modeling were used to identify meaningful differences between subject groups as a function of signal distortion caused by frequency compression and noise. Results: Working memory was a significant factor in listeners’ intelligibility of sentences presented in babble noise and processed with frequency compression based on sinusoidal modeling. At maximum signal modification (caused by both frequency compression and babble noise), the factor of working memory (when controlling for age and hearing loss) accounted for 29.3% of the variance in intelligibility scores. Combining working memory, age, and hearing loss accounted for a total of 47.5% of the variability in intelligibility scores. Furthermore, as the total amount of signal distortion increased, listeners with higher working memory performed better on the intelligibility task than listeners with lower working memory did. Conclusions: Working memory is a significant factor in listeners’ responses to total signal distortion caused by cumulative effects of babble noise and frequency compression implemented with sinusoidal modeling. These results, together with other studies focused on wide-dynamic range compression, suggest that older listeners with hearing loss and poor working memory are more susceptible to distortions caused by at least some types of hearing aid signal-processing algorithms and by noise, and that this increased susceptibility should be considered in the hearing aid fitting process.
Spoken language production in adults: Examining age-related differences in syntactic complexity

Abstract The use of complex syntax in the spoken discourse of young, middle-aged, and older adults was examined. The purpose of the study was to determine if syntactic complexity would show an age-related decline. Language samples were elicited from healthy adults in their 20 s, 40 s, and 60 s (n = 20 per group) using a conversational (CON) task and a peer conflict resolution (PCT) task. Each sample was transcribed, segmented into utterances, and coded for main clauses and all types of finite and non-finite subordinate clauses. The results indicated that all groups produced greater syntactic complexity during the PCR task than the CON task as measured by mean length of utterance, clausal density, and the use of left-branching clauses. Additionally, the groups did not differ on any syntactic measures for either task. It is concluded that syntactic complexity in spoken discourse remains stable during the years that span early, middle, and later adulthood.
Effect of silver nanoparticles on growth performance, metabolism and microbial profile of broiler chickens

This study evaluated the potential of silver nanoparticles (AgNano) as an antimicrobial growth-promoting supplement for broiler chickens. One hundred forty-four seven-day-old broiler chicks were distributed randomly to AgNano treatments at 0, 10 and 20 mg/kg (Control, Group AgNano10, and Group AgNano20, respectively) provided via the drinking water from day 7 to 36 post-hatching. Body weight and feed consumption were measured weekly. In addition, balance and respiration experiments were carried out to determine nitrogen (N) utilisation and energy retention. At days 22 and 36, blood samples and intestinal content were collected to evaluate the effects of AgNano on plasma concentration of immunoglobulins and the intestinal microflora, repectively. The provision of water solutions containing different concentrations of AgNano had no effect on postnatal growth performance and the energy metabolism of broiler chickens. However, in Group AgNano10 N intake (p = 0.05) and retention (p = 0.03) was increased, but N excretion and efficiency of utilisation was not affected. The populations of bacteria in the intestinal samples were not affected by AgNano supplementation. The concentration of immunoglobulin (IgG) in the blood plasma of broilers supplemented with AgNano decreased at day 36 (p = 0.012). The results demonstrated that AgNano affects N utilisation and plasma IgG concentration; however, it does not influence the microbial populations in the digestive tract, the energy metabolism and growth performance of chickens.
Windows of vulnerability to psychopathology and therapeutic strategy in the adolescent rodent model

Adolescence comes in association with puberty, when maturation and rearrangement of major neurotransmitter pathways and functions are still taking place. The neurobiological processes occurring in the brain during this developmental period have been so far poorly investigated. Yet, it is during adolescence that some major neuropsychiatric disorders may become evident, including ADHD, schizophrenia, and drug abuse. Moreover, the age-related neurobehavioural plasticity renders adolescents particularly vulnerable to the consequences of psychoactive drug exposure. In this view, there is an increased likelihood that addiction will develop when psychoactive drug use starts early during adolescence. From all these observations adolescence emerges as a ‘critical’ phase in development. In the present review, we focus on recent neurobiological characterization of adolescent rats and mice. As for vulnerability to addictive behaviour, nicotine exposure during adolescence dose-dependently down-regulated levels of AMPA GluR2/3 subunits in the striatum, suggesting a reduced neurobehavioural plasticity in adult subjects. Comparable exposure during adulthood had opposite effects. It was found consistently that exposure to nicotine during adolescence, but not similar exposure in the post-adolescent period, increased the expression of specific subunits of the acetylcholine receptor in adult rats, thus enhancing the reinforcing efficacy of nicotine in a self-administration paradigm. The present data identified a specific age-window, characterized by long-term effects on behavioural and neurochemical indexes, of vulnerability. With respect to potential therapeutic approaches in ADHD, we studied the adolescent spontaneously-hypertensive-rat (SHR) in an intolerance-to-delay operant-behaviour paradigm. The model was further validated by the finding that impulsivity was reduced by chronic methylphenidate administration. ‘Impulsive’ SHR animals were characterized by reduced cannabinoid CB1 receptor density in the prefrontal cortex. Interestingly, an acute cannabinoid agonist increased levels of self-control behaviour in these animals. The present data suggest that pharmacological modulation of the cannabinoid system might improve some behavioural anomalies seen in ADHD. In conclusion, modelling the adolescent phase in rats and mice appears to be useful for the investigation of determinants of vulnerability to addiction and to other early-onset neuropsychiatric disorders.
Effect of growth conditions on the Streptococcus bovis phosphoenolpyruvate glucose phosphotransferase system.

Four strains of the ruminal bacterium Streptococcus bovis were surveyed for phosphoenolpyruvate (PEP) and ATP-dependent phosphorylation of glucose and the nonmetabolizable glucose analog 2-deoxyglucose. All four strains had high rates of glucose phosphorylation with either phosphoryl donor, but 2-deoxyglucose activity was much higher in the presence of PEP. These results provide evidence for a PEP-dependent glucose phosphotransferase system in these bacteria. Mannose and 2-deoxyglucose inhibited PEP-dependent phosphorylation of glucose by S. bovis JB1 by 50 and 38%, respectively, whereas alpha-methylglucoside had little effect. Mannose was a competitive inhibitor of PEP-dependent phosphorylation of glucose with an inhibition constant of 2.8 mM, and PEP-dependent activity in cells grown in batch culture was optimal at pH 7.2. When S. bovis JB1 was grown in continuous culture, PEP-dependent phosphorylation of glucose and 2-deoxyglucose was highest in cells grown at a dilution rate of . 10/h and at low glucose concentrations. Phosphoenolpyruvate-dependent activity was optimum at a growth pH of 5.0 for cells grown in medium that contained less than 6.0 g/liter of glucose. These data indicate that PEP-dependent glucose phosphotransferase system activity can be influenced depending on the growth conditions used to culture S. bovis. Furthermore, these results suggest that environmental conditions within the rumen will affect how glucose is transported by S. bovis.