Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Chronic Typical and Atypical Neuroleptic Treatment on Proenkephalin mRNA Levels in the Striatum and Nucleus Accumbens of the Rat

Abstract: We measured proenkephalin (PEK) mRNA levels in the anterior and medial aspects of the caudate‐putamen (CPU) and in the nucleus accumbens (NAc) of the rat by in situ hybridization histochemistry after chronic treatment for 21 days with typical (haloperidol and prolixin) and atypical (molindone, thioridazine, and clozapine) neuroleptics. Chronic administration with these drugs resulted in PEK mRNA levels that were 60–80% higher than controls in the anterior and medial aspects of the CPU but only 25–30% over controls in the NAc. All three atypical neuroleptics studied increased PEK mRNA in the following order: anterior‐CPU, thioridazine > clozapine and molindone; medial‐CPU, thioridazine and molindone > clozapine; and NAc, thioridazine > > molindone and clozapine. Chronic treatment with the specific dopamine D2 antagonist sulpiride also caused elevation in PEK mRNA levels in all three brain regions studied whereas the specific serotonin S2 receptor blocker, cinanserin, had no significant effects on PEK mRNA levels. These results are consistent with the hypothesis that elevated levels of the enkephalins in the mesolimbic system may be necessary for antipsychotic activity. They also support the idea that the undesirable motoric signs and symptoms observed after chronic treatment with typical neuroleptics may not be the result of increased levels of enkephalins in the basal ganglia because atypical neuroleptics which are almost totally devoid of these side effects caused similar increases in PEK mRNA in the CPU.
Social participation and quality-of-life of patients with traumatic brain injury living in the community: A mixed methods study

Abstract Objective: To investigate the quality-of-life (QoL) and social participation of patients with traumatic brain injury (TBI) living in the community in Japan. Methods: A mixed-methods study of 29 post-TBI patients and 12 family members was conducted. Objective scales were used to evaluate QoL (Short Form Health Survey SF-36), depression (Zung Self-rating Depression Scale) and psychosocial function (Sydney Psychosocial Reintegration Scale, 2nd edition). Subjective views of changes in social functioning, participation and suitability of family support were obtained by a semi-structured interview. Participants were classified into ‘change’ and ‘no-change’ groups for social participation and between-group comparisons of QoL and determinant factors of QoL were evaluated. Results: The SF-36 social role component was significantly associated with the suitability of family support, followed by their understanding. However, QoL was not significantly associated with changes in social participation. Social participation was particularly influenced by the absence of rehabilitation support for low-skill labourers and housewives, whether they lost their employment or not. Conclusions: Advances in rehabilitation services are required, particularly to meet the specific needs of housewives and low-skill labourers. Families should receive sufficient education and short- and long-term strategies for providing suitable support to patients and their families should be implemented.
First-in-Human Clinical Trial of Oral ONC201 in Patients with Refractory Solid Tumors

Purpose: ONC201 is a small-molecule selective antagonist of the G protein–coupled receptor DRD2 that is the founding member of the imipridone class of compounds. A first-in-human phase I study of ONC201 was conducted to determine its recommended phase II dose (RP2D). Experimental Design: This open-label study treated 10 patients during dose escalation with histologically confirmed advanced solid tumors. Patients received ONC201 orally once every 3 weeks, defined as one cycle, at doses from 125 to 625 mg using an accelerated titration design. An additional 18 patients were treated at the RP2D in an expansion phase to collect additional safety, pharmacokinetic, and pharmacodynamic information. Results: No grade >1 drug-related adverse events occurred, and the RP2D was defined as 625 mg. Pharmacokinetic analysis revealed a Cmax of 1.5 to 7.5 μg/mL (∼3.9–19.4 μmol/L), mean half-life of 11.3 hours, and mean AUC of 37.7 h·μg/L. Pharmacodynamic assays demonstrated induction of caspase-cleaved keratin 18 and prolactin as serum biomarkers of apoptosis and DRD2 antagonism, respectively. No objective responses by RECIST were achieved; however, radiographic regression of several individual metastatic lesions was observed along with prolonged stable disease (>9 cycles) in prostate and endometrial cancer patients. Conclusions: ONC201 is a selective DRD2 antagonist that is well tolerated, achieves micromolar plasma concentrations, and is biologically active in advanced cancer patients when orally administered at 625 mg every 3 weeks. Clin Cancer Res; 23(15); 4163–9. ©2017 AACR.
A New Method for Unit Commitment at Electricite De France

Unit commitment is a complex problem which, until now and for real size systems, has been solved using heuristic methods only. This paper proposes a new Lagrangian relaxation method, which produces ¿-optimal solutions. The flexibility of the algorithm is such that numerous developments can be envisaged, such as simultaneous management of pumping units, probabilistic determination of the spinning reserve, etc.
Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (penA) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime

ABSTRACT Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 μg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 μg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.
Author ' s personal copy Analysis of adenoviruses and polyomaviruses quantified by qPCR as indicators of water quality in source and drinking-water treatment plants

Three drinking-water treatment plants were analyzed for the presence of human adenoviruses (HAdV) and JC polyomavirus (JCPyV), previously suggested as viral contamination indicators, in order to define their water quality in relation to the presence of viral pathogens and the efficiency of the treatments applied. The 90% of the river water samples had positive results of HAdV (10–10 genome copies (GC)/L); and 48%, of JCPyV (10–10 GC/L). Lower concentrations of HAdV and JCPyV were found in different treatment steps of the plants in absence of bacterial standards. Virus removal efficiencies were higher than 5 logs in plants 1 and 3 and could be quantified as >2 logs in plant 2. However, three post-chlorinated samples from plants 2 and 3 (11%) were found to be positive for HAdV by qPCR, but did not show infectivity in the cell cultures assayed. Simple methods based on the adsorption–elution of viruses from glass wool give low-cost and efficient virus recovery from source water and large-volume water samples. Quantification of JCPyV and HAdV using qPCR is useful for evaluating virus removal efficiency in water treatment plants, identification of Hazard Analysis and Critical Control Points (HACCP) and as a molecular index of the virological quality of water. Though infectivity is not guaranteed when using qPCR techniques in water treated with disinfection processes, the quality of source water, where viruses have proved to have infective capabilities, and the removal efficiency of viral particles may be efficiently quantified. a 2009 Elsevier Ltd. All rights reserved.
Comparison and Evaluation of Three Methods for Estimating Forest above Ground Biomass Using TM and GLAS Data

Medium spatial resolution biomass is a crucial link from the plot to regional and global scales. Although remote-sensing data-based methods have become a primary approach in estimating forest above ground biomass (AGB), many difficulties remain in data resources and prediction approaches. Each kind of sensor type and prediction method has its own merits and limitations. To select the proper estimation algorithm and remote-sensing data source, several forest AGB models were developed using different remote-sensing data sources (Geoscience Laser Altimeter System (GLAS) data and Thematic Mapper (TM) data) and 108 field measurements. Three modeling methods (stepwise regression (SR), support vector regression (SVR) and random forest (RF)) were used to estimate forest AGB over the Daxing’anling Mountains in northeastern China. The results of models using different datasets and three approaches were compared. The random forest AGB model using Landsat5/TM as input data was shown the acceptable modeling accuracy (R2 = 0.95 RMSE = 17.73 Mg/ha) and it was also shown to estimate AGB reliably by cross validation (R2 = 0.71 RMSE = 39.60 Mg/ha). The results also indicated that adding GLAS data significantly improved AGB predictions for the SVR and SR AGB models. In the case of the RF AGB models, including GLAS data no longer led to significant improvement. Finally, a forest biomass map with spatial resolution of 30 m over the Daxing'anling Mountains was generated using the obtained optimal model.
Core temperature and hydration status during an Ironman triathlon

Background: Numerous laboratory based studies have documented that aggressive hydration strategies (∼1–2 litres/h) are required to minimise a rise in core temperature and minimise the deleterious effects of hyperthermia on performance. However, field data on the relations between hydration level, core body temperature, and performance are rare. Objective: To measure core temperature (Tcore) in triathletes during a 226 km Ironman triathlon, and to compare Tcore with markers of hydration status after the event. Method: Before and immediately after the 2004 Ironman Western Australia event (mean (SD) ambient temperature 23.3 (1.9)°C (range 19–26°C) and 60 (14)% relative humidity (44–87%)) body mass, plasma concentrations of sodium ([Na+]), potassium ([K+]), and chloride ([Cl−]), and urine specific gravity were measured in 10 well trained triathletes. Tcore was measured intermittently during the event using an ingestible pill telemetry system, and heart rate was measured throughout. Results: Mean (SD) performance time in the Ironman triathlon was 611 (49) minutes; heart rate was 143 (9) beats/min (83 (6)% of maximum) and Tcore was 38.1 (0.3)°C. Body mass significantly declined during the race by 2.3 (1.2) kg (−3.0 (1.5)%; p<0.05), whereas urine specific gravity significantly increased (1.011 (0.005) to 1.0170 (0.008) g/ml; p<0.05) and plasma [Na+], [K+], and [Cl−] did not change. Changes in body mass were not related to finishing Tcore (r = −0.16), plasma [Na+] (r = 0.31), or urine specific gravity (r = −0.37). Conclusion: In contrast with previous laboratory based studies examining the influence of hypohydration on performance, a body mass loss of up to 3% was found to be tolerated by well trained triathletes during an Ironman competition in warm conditions without any evidence of thermoregulatory failure.
The effect of the prenatal and post-natal long-term exposure to 50 Hz electric field on growth, pubertal development and IGF-1 levels in female Wistar rats

To investigate prenatal and post-natal effects of extremely low frequency (ELF) electric field (EF) on growth and pubertal development, pregnant Wistar rats were randomly distributed among three groups. The pregnant rats of the prenatal group were exposed to 24-hour EF at 50 Hz EF 10 kV/min during pregnancy and their subsequent randomly selected female pups continued to be exposed until puberty. The post-natal group was unexposed to EF during pregnancy, but randomly selected female pups from this group were exposed to EF between delivery and puberty at the same doses and duration as the prenatal group. The third group was a sham-exposed group. The mean birth weight and weight gain of the pups during study period were found significantly reduced in prenatal group than post-natal and sham-exposed groups (p < 0.001). No difference could be found among the three groups for body weight at puberty (p > 0.05). The mean age at vaginal opening and estrous were significantly higher at prenatal group than post-natal and sham-exposed groups (p < 0.001). Serum insulin-like growth hormone-1 (IGF-1) levels were found significantly reduced in prenatal exposure group compared with the other two groups (p < 0.001). There was no difference for birth weight, weight gain, the mean age at vaginal opening and estrous and IGF-1 levels between post-natal and sham-exposed groups (p > 0.05). There was also no difference for FSH, LH and E2 levels at puberty among the three groups (p > 0.05). Histological examination revealed that both the prenatal and post-natal groups had the evidence of tissue damage on hypothalamus, pituitary gland and ovaries. In conclusion, early beginning of prenatal exposure of rats to 24 hours 50 Hz EF at 10 kV/m until puberty without magnetic field (MF) resulted in growth restriction, delayed puberty and reduced IGF-1 levels in female Wistar rats. These effects probably associated with direct toxic effects of EF on target organs. Post-natal exposure to EF at similar doses and duration seems to be less harmful on target organs. Post-natal exposure to EF at similar doses and duration seems to be less harmful.
BPM governance: a literature analysis of performance evaluation

Purpose The purpose of this paper is to collect data and select a bibliographic portfolio (BP) of the literature on the performance evaluation of business process management (BPM) governance, in order to highlight studies aligned with business process governance, and with scientific recognition and its bibliometric parameters. Design/methodology/approach The research method is qualitative and the process of identification, selection, and analysis of the articles in the BP took place through evaluation and interpretation by the authors of this research. Regarding data collection, this research used primary data in the selection of the BP, and secondary data when the authors analyzed the articles. Findings The paper selects a set of 19 articles with scientific recognition and aligned with the research topic, which refers to the performance evaluation of BPM governance. This set is called the BP, and highlights and contains the most often present journals, articles, authors, and keywords found in the search performed by the authors. Originality/value This research uses thorough procedures for the selection process that differ from traditional procedures: the selection process is structured in a way that allows checking of each research stage; the research topic is fragmented into many areas of study and the search is carried out simultaneously in all these areas; and the researchers interact with the search process and as they expand their learning they adjust the search direction.
Protein kinase C redistribution within CA3 stratum oriens during acquisition of nictitating membrane conditioning in the rabbit.

This manuscript describes experiments designed to investigate protein kinase C redistribution occurring during acquisition of the rabbit nictitating membrane (NM) conditioned response (CR). The first experiment defined the acquisition phase of the NM response for our laboratory. A group of rabbits (n = 6) was given 2 days of paired NM training; a second group (n = 6) was given 2 days of unpaired NM training. The data document a variable level of responding on day 1 for rabbits given paired training (mean +/- SEM, 21 +/- 11% CRs) but show that on day 2 most rabbits reached the behavioral asymptote (five of six rabbits responding with greater than 85% CRs). Rabbits responding at the behavioral asymptote were defined as having acquired the NM conditioned response. These data were interpreted to indicate that 1 day of training initiated processes necessary for behavioral acquisition (i.e., responding at the behavioral asymptote). A quantitative film autoradiographic study of [3H]phorbol 12,13-dibutyrate binding was then used to determine the distribution of hippocampal protein kinase C in rabbits sacrificed after receiving either 1 day of paired stimuli (n = 10), 1 day of unpaired stimuli (n = 6), or no stimuli (n = 6). Autoradiograms were analyzed by measuring binding in strictly defined regions of interest and from transept profiles. A significant increase in binding of the phorbol ester was found in the CA3 stratum oriens in the paired group relative to unpaired and naive controls. No other significant differences were found.
An optimal current reference generation method for IPM brushless machines with non-sinusoidal back-EMF

Interior permanent magnet (IPM) brushless machines with non-sinusoidal back-EMF offer advantages of higher torque density and cost effectiveness, compared to the sinusoidal PM brushless machines. However, these machines suffer from higher torque ripple when being fed by most applied sinusoidal or square-wave currents. This problem can be solved by optimizing the current waveforms. Based on instantaneous power theory, a novel offline method to generate the optimal current references for non-sinusoidal IPM brushless machines is proposed. When the optimal currents are injected to the IPM machines, the torque ripple will be minimized. Meanwhile, the maximum torque per ampere control can be fulfilled and energy efficiency will be enhanced. A closed form solution for the optimal current references is deduced on the basis of mathematically modeling and instantaneous power analysis of the IPM machines. Finally, the effectiveness of the proposed method is validated through finite element simulation of a non-sinusoidal IPM brushless machine prototype.
Comparative effects of glucagon-like peptide-2 (GLP-2), growth hormone (GH), and keratinocyte growth factor (KGF) on markers of gut adaptation after massive small bowel resection in rats.

BACKGROUND Administration of specific growth factors exert gut-trophic effects in animal models of massive small bowel resection (SBR); however, little comparative data are available. Our aim was to compare effects of a human glucagon-like peptide-2 (GLP-2) analog, recombinant growth hormone (GH) and recombinant keratinocyte growth factor (KGF) on jejunal, ileal, and colonic growth and functional indices after 80% SBR in rats. METHODS Thirty-seven male rats underwent small bowel transection (sham operation) with s.c. saline administration (control; Tx-S; n = 7) or 80% midjejuno-ileal resection (Rx) and treatment with either s.c. saline (Rx-S, n = 7), GLP-2 at 0.2 mg/kg/d (Rx-GLP-2; n = 8), GH at 3.0 mg/kg/d (Rx-GH; n = 8), or KGF at 3.0 mg/kg/d (Rx-KGF; n = 7) for 7 days. All groups were pair-fed to intake of Rx-S rats. Gut mucosal cell growth indices (wet weight, DNA and protein content, villus height, crypt depth, and total mucosal height) were measured. Expression of the cytoprotective trefoil peptide TFF3 was determined by Western blot. Gut mucosal concentrations of the tripeptide glutathione (L-glutamyl-L-cysteinyl-glycine) and glutathione disulfide (GSSG) were measured by high-performance liquid chromatography and the glutathione/GSSG ratio calculated. RESULTS SBR increased adaptive growth indices in jejunal, ileal, and colonic mucosa. GLP-2 treatment increased jejunal villus height and jejunal total mucosal height compared with effects of resection alone or resection with GH or KGF treatment. Both GH and KGF modestly increased colonic crypt depth after SBR. SBR did not affect small bowel or colonic goblet cell number or TFF3 expression; however, goblet cell number and TFF3 expression in both small bowel and colon were markedly up-regulated by KGF treatment and unaffected by GLP-2 and GH. SBR oxidized the ileal and colonic mucosal glutathione/GSSG redox pools. GLP-2 treatment after SBR increased the glutathione/GSSG ratio in jejunum, whereas KGF had an intermediate effect. In addition, GLP-2 (but not GH or KGF) prevented the SBR-induced oxidation of the glutathione/GSSG pools in both ileum and colon. CONCLUSIONS GLP-2 exerts superior trophic effects on jejunal growth and also improves mucosal glutathione redox status throughout the bowel after massive SBR in rats. Both GH and KGF increase colonic mucosal growth in this model. KGF alone potently increases gut mucosal goblet cell number and expression of the cytoprotective trefoil peptide TFF3. The differential effects of GLP-2, GH and KGF administration in this model of short bowel syndrome suggest that individual therapy with these growth factors may not be an adequate strategy to maximally improve adaptive gut mucosal growth and cytoprotection after massive small intestinal resection. Future research should address the use of these agents in combination in short bowel syndrome.
Vaccinia Virus Uses Macropinocytosis and Apoptotic Mimicry to Enter Host Cells

http://www.sciencemag. org/cgi/content/full/320/5875/531 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag. org/cgi/content/full/320/5875/531/DC1 can be found at: Supporting Online Material found at: can be related to this article A list of selected additional articles on the Science Web sites http://www.sciencemag. org/cgi/content/full/320/5875/531#related-content http://www.sciencemag. org/cgi/content/full/320/5875/531#otherarticles , 14 of which can be accessed for free: cites 25 articles This article 6 article(s) on the ISI Web of Science. cited by This article has been http://www.sciencemag. org/cgi/content/full/320/5875/531#otherarticles 3 articles hosted by HighWire Press; see: cited by This article has been http://www.sciencemag. org/cgi/collection/virology Virology : subject collections This article appears in the following
Environmental and Physiological Factors Contributing to Outbreaks of Cryptosporidium in Coquerel's Sifaka (Propithecus coquereli) at the Duke Lemur Center: 1999–2007

Abstract This population-based, retrospective study examined the susceptibility of a prosimian primate, Coquerel's sifaka (Propithecus coquereli), to Cryptosporidium spp. over a 9-yr period from 1999 to 2007 at the Duke Lemur Center (DLC) located in Durham, North Carolina. The investigation examined potential epidemiologic risk factors that could be correlated to infectious outbreaks at the center, such as prevalence, signalment (species, age, and sex), seasonality of occurrence, recurrence rate, family lineage, parturition, clinical signs, and concurrent diseases or health conditions. Findings included Propithecus spp. being the only lemur species at the DLC showing clinical signs of infection, with age being an important factor in susceptibility, and showing a strong correlation between temperature and seasonality with shedding of Cryptosporidium oocysts. These findings present new information regarding cryptosporidiosis in captive prosimians.