Miracle-dz/UniFAR-FTUs huggingface dataset:
Mycoplasma hyopneumoniae p65 Surface Lipoprotein Is a Lipolytic Enzyme with a Preference for Shorter-Chain Fatty Acids

ABSTRACT Mycoplasma hyopneumoniae is the most significant bacterial pathogen of the respiratory tract of swine. p65 is an immunodominant surface lipoprotein of M. hyopneumoniae that is specifically recognized during disease. Analysis of the translated amino acid sequence of the gene encoding p65 revealed similarity to the GDSL family of lipolytic enzymes. To examine the lipolytic activity of p65, the gene was cloned and expressed in Escherichia coli after truncation of the prokaryotic lipoprotein signal sequence and mutagenesis of the mycoplasma TGA tryptophan codons. After treatment with thrombin, the recombinant glutathione S-transferase (GST)-p65 protein yielded a 66-kDa fusion protein cleavage product corresponding in size to the mature p65 protein. The esterase activity of recombinant GST-p65 was indicated by the formation of a cleared zone on tributyrin agar plates and the hydrolysis of p-nitrophenyl esters of caproate (pNPC) and p-nitrophenyl esters of palmitate (pNPP). Lipase activity was indicated by the hydrolysis of the artificial triglyceride 1,2-O-dilauryl-rac-glycero-3-glutaric acid resorufin ester. Using pNPC and pNPP as substrates, recombinant GST-p65 had optimal activity between pHs 9.2 and 10.2 and at a temperature higher than 39°C. Calcium ions did not increase the activity of recombinant GST-p65. Rabbit anti-p65 antibodies inhibited the activity of recombinant GST-p65 and also inhibited the growth of M. hyopneumoniae in vitro. Examination of the kinetic parameters of recombinant GST-p65 for the hydrolysis of pNPC and pNPP indicated a preference for the shorter fatty acid chain of pNPC. The physiological and/or pathogenic role of mycoplasma lipolytic enzymes has not been determined, but they are likely to play an important role in mycoplasmas' nutritional requirements for long-chain fatty acids and may reduce the function of lung surfactants in mycoplasma-induced respiratory diseases. This is the first report of the lipolytic activity of a lipid-modified surface immunogen of a mycoplasma.
LMDS radio channel obstructed by foliage

This paper investigates an obstructed radio channel by foliage for LMDS (local multipoint distribution services) where a relative evaluation of attenuation characteristics of foliage at 29.5 and 5 GHz is performed. Results show that the attenuation in dB should be treated statistically as Rician distribution. It is also found that swaying foliage in wind causes a significant channel fading at 29.5 GHz, ranging over 10 dB, while the fading depth at 5 GHz is approximately 2 dB.
Development, Characterization, and Use of a Fetal Skin Cell Bank for Tissue Engineering in Wound Healing

Wound healing in fetal skin is characterized by the absence of scar tissue formation, which is not dependent on the intrauterine environment and amniotic fluid. Fetal cells have the capacity of extraordinary expansion and we describe herein the development of a fetal skin cell bank where from one organ donation (2–4 cm2) it is possible to produce several hundred million fetal skin constructs of 9 × 12 cm2. Fetal cells grow three to four times more rapidly than older skin cells cultured in the same manner and these banked fetal cells are very resistant against physical and oxidative stress when compared to adult skin cells under the same culture conditions. They are up to three times more resistant to UVA radiation and two times more resistant towards hydrogen peroxide treatment. This mechanism may be of major importance for fetal cells when they are delivered to hostile wound environments. For fetal cell delivery to patients, cells were associated with a collagen matrix to form a three-dimensional construct in order to analyze the capacity of these cells for treating various wounds. We have seen that fetal cells can modify the repair response of skin wounds by accelerating the repair process and reducing scarring in severe burns and wounds of various nature in children. Hundreds of thousands of patients could potentially be treated for acute and chronic wounds from one standardized and controlled cell bank.
Several signaling pathways are involved in the control of cattle oocyte maturation

The main limit of in vitro production of domestic mammal embryos comes from the low capacity of in vitro matured oocytes to develop after fertilization. As soon as they are separated from follicular environment, oocytes spontaneously resume meiosis without completion of their terminal differentiation. Roscovitine (ROS), an inhibitor of M‐phase promoting factor (MPF) kinase activity reversibly blocks the meiotic resumption in vitro. However, in cattle maturing oocytes several cellular events such as protein synthesis and phosphorylation, chromatin condensation and nuclear envelope folding escape ROS inhibition suggesting the alternative pathways in oocyte maturation. We compared the level of synthesis and phosphorylation of several protein kinases during bovine cumulus oocyte complex (COC) maturation in vitro in the presence or not of epidermal growth factor (EGF) and ROS. We showed that during the EGF‐stimulated maturation, ROS neither affected the decrease of EGF receptor (EGFR) nor did inhibit totally its phosphorylation in cumulus cells and also did not totally eliminate tyrosine phosphorylation in oocytes. However, ROS did inhibit the Phosphoinositide 3‐kinase (PI3) activity when oocytes mature without EGF. Accumulation of Akt/PKB (protein kinase B), JNK1/2 (jun N‐terminal kinases) and Aurora‐A in oocytes during maturation was not affected by ROS. However, the phosphorylation of Akt but not JNKs was diminished in ROS‐treated oocytes. Thus, PI3 kinase/Akt, JNK1/2 and Aurora‐A are likely to be involved in the regulation of bovine oocyte maturation and some of these pathways seem to be independent to MPF activity and meiotic resumption. This complex regulation may explain the partial meiotic arrest of ROS‐treated oocytes and the accelerated maturation observed after such treatment. Mol. Reprod. Dev. 69: 466–474, 2004. © 2004 Wiley‐Liss, Inc.
Gender Based Violence in Nigeria: The Case of Sexual Harassment in Tertiary Institutions

From the cultural perspective, the practice of gender based violence is predominant in the world societies, especially developing countries like Nigeria. One of the identifiable forms of GBV in schools is sexual harassment or abuse. This activity endangers the girls in school and often times engender poor physical and psychological health, even death: GBV therefore remains an obstacle to girls' education. The paper proceeded through a library or secondary search-methodology. It also attempted to evaluate the school activity and let down intervention mechanisms. The paper concludes with a proposal for the enforcement of available laws and repealing of obsolete ones so as to dismantle all forms of impediments against the development/advancement of girls '/women education in Nigerian institutions.
Critical success factors for behavior-based safety: A study of twenty industry-wide applications1

One-on-one interviews and focus-group meetings were held at 20 organizations that had implemented a behavior-based safety (BBS) process in order to find reasons for program successes/failures. A total of 31 focus groups gave 629 answers to six different questions. A content analysis of these responses uncovered critical information for understanding what employees are looking for in a BBS program. A perception survey administered to individual employees (n=701) at these organizations measured a variety of variables identified in prior research to influence success in safety efforts. The survey data showed five variables to be significantly predictive of employee involvement in a BBS process: 1) perceptions that BBS training was effective, 2) trust in management abilities, 3) accountability for BBS through performance appraisals, 4) whether or not one had received education in BBS, and 5) tenure with the organization. Also, employees in organizations mandating employee participation in a BBS process (n=8 companies) reported significantly higher levels of a) involvement, b) trust in management, c) trust in coworkers, and d) satisfaction with BBS training than did employees whose process was completely voluntary (n=12 companies). In addition, employees in mandatory processes reported significantly greater frequency of giving and receiving positive behavior-based feedback.
Gut permeability and food allergies

Intestinal permeability is a critical feature of the gastrointestinal epithelium as it must allow an efficient passage of nutrients and restrict the entry of larger molecules, such as protein antigen, in order to facilitate appropriate immune responses towards food antigens. The proper regulation of the epithelial barrier relies on multiple, intricate physiological and immunologic mechanisms, in terms of which recent progresses regarding the cellular and molecular components have been unravelled. In genetically predisposed individuals, breakdown of oral tolerance can occur, leading to the inadequate production of allergen‐specific IgE and the recruitment of mast cells in the gastrointestinal mucosa. Under such conditions, the intestinal permeability towards allergen is altered via different mechanisms, with IgE–CD23‐mediated transport across the mucosa playing an important amplification role. Additionally, during the effector phase of the allergic reaction, when mast cells degranulate, a series of inflammatory mediators, such as proteases and cytokines, are released and further affects intestinal permeability. This leads to an increase in the passage of allergens and hence contributes to perpetuate the inflammatory reaction. In this review, we describe the importance of properly balanced intestinal permeability in oral tolerance induction and address the processes involved in damaging the intestinal barrier in the sensitized epithelium and during allergic reactions. We conclude by speculating on the effect of increased intestinal permeability on the onset of sensitization towards dietary antigens.
Similarities between the antABC-Encoded Anthranilate Dioxygenase and the benABC-Encoded Benzoate Dioxygenase of Acinetobacter sp. Strain ADP1

ABSTRACT Acinetobacter sp. strain ADP1 can use benzoate or anthranilate as a sole carbon source. These structurally similar compounds are independently converted to catechol, allowing further degradation to proceed via the β-ketoadipate pathway. In this study, the first step in anthranilate catabolism was characterized. A mutant unable to grow on anthranilate, ACN26, was selected. The sequence of a wild-type DNA fragment that restored growth revealed theantABC genes, encoding 54-, 19-, and 39-kDa proteins, respectively. The deduced AntABC sequences were homologous to those of class IB multicomponent aromatic ring-dihydroxylating enzymes, including the dioxygenase that initiates benzoate catabolism. Expression of antABC in Escherichia coli, a bacterium that normally does not degrade anthranilate, enabled the conversion of anthranilate to catechol. Unlike benzoate dioxygenase (BenABC), anthranilate dioxygenase (AntABC) catalyzed catechol formation without requiring a dehydrogenase. In Acinetobacter mutants,benC substituted for antC during growth on anthranilate, suggesting relatively broad substrate specificity of the BenC reductase, which transfers electrons from NADH to the terminal oxygenase. In contrast, the benAB genes did not substitute for antAB. An antA point mutation in ACN26 prevented anthranilate degradation, and this mutation was independent of a mucK mutation in the same strain that prevented exogenous muconate degradation. Anthranilate induced expression of antA, although no associated transcriptional regulators were identified. Disruption of three open reading frames in the immediate vicinity ofantABC did not prevent the use of anthranilate as a sole carbon source. TheantABC genes were mapped on the ADP1 chromosome and were not linked to the two known supraoperonic gene clusters involved in aromatic compound degradation.
Development and Psychometrics of Instruments to Assess School Personnel’s Bystander Action in Situations of Teen Relationship Abuse and Sexual Assault

This article describes recently developed instruments that assess school personnel’s bystander barriers and intentions in situations of teen relationship abuse, sexual assault, and sexual harassment, as well as perceptions of school readiness specific to relationship abuse, sexual assault, and sexual harassment prevention and response. Participants were 1,150 high school personnel from 25 schools in New Hampshire, Massachusetts, and Maine. Specific instruments focused on bystander intentions, bystander action, barriers to bystander action, and perceptions of school readiness. Participants were randomly divided into two groups for analysis—the exploratory sample (ES; n = 575) and the confirmatory sample (CS; n = 575). Overall, the measures demonstrated acceptable fit indices. Results suggested that most measures and subscales had adequate reliability, but a few subscales had less than ideal internal consistency, which can likely be attributed to the small number of items. More work is needed, but these measures act as a starting point by which the role of school personnel in prevention initiatives and bystander intervention can be evaluated.
Improvement in CNS protective treatment in non-high-risk childhood acute lymphoblastic leukemia: report from the Japanese Children's Cancer and Leukemia Study Group.

BACKGROUND Prevention of central nervous system (CNS) leukemia by early introduction of therapy to this sanctuary site is an essential component of modern treatment strategy for acute lymphoblastic leukemia (ALL). However, the optimal form of preventive CNS therapy remains debatable. PROCEDURE To address this issue, we evaluated the efficacy of CNS preventive therapy for 572 children with ALL who achieved complete remission in the Children's Cancer and Leukemia Study Group (CCLSG) ALL874 (1987-1990) and ALL911 (1991-1993) studies. They received risk-directed therapy based on age and leukocyte count. In the ALL 874 study, the non-high-risk (low-risk [LR] + intermediate risk [IR]) patients were randomly assigned to the conventional cranial irradiation (CRT) regimen (L874A and I874A) and the high-dose methotrexate (HDMTX) regimen without CRT (L874B and I874B). The former patients received 18-Gy CRT plus 3 doses of intrathecal (i.t.) MTX and the latter patients received 3 courses of HDMTX at 2 g/m2 plus 13 doses of ITMTX (L874B) or 4 courses of HDMTX at 4.5 g/m2 plus 1 dose of ITMTX (I874B). RESULTS The 7-year probabilities (+/- SE) of CNS relapse-free survival were 97.3% +/- 2.6% (L874A, n = 41) vs. 90.3% +/- 5.3% (L874B, n = 39) (P = 0.25) in the LR patients, and 100% (I874A, n = 55) vs. 78.5% +/- 6.5% (I874B, n = 54) (P = 0.002) in the IR patients. The corresponding disease-free survival (DFS) rates were 79.4% +/- 6.5% vs. 74.4% +/- 7.3% (P = 0.62) in the LR group and 63.3% +/- 6.8% vs. 58.3% +/- 7.2% (P = 0.66) in the IR group. Thus, the HDMTX regimen could not provide better protection of CNS relapse as compared with the CRT regimen, although their overall efficacy was not significantly different. In the ALL 911 study, intensive systemic chemotherapy with extended i,t, injections of MTX plus cytarabine achieved a high CNS relapse-free survival (98% +/- 1.9% at 7 years) and a favorable DFS (85.5% +/- 5% at 7 years) in the IR patients. The patients in the high-risk (HR) group in both ALL874 and ALL911 studies received the 18-Gy or 24-Gy CRT with intensive systemic chemotherapy. Their 7-year probabilities of CNS relapse-free survival ranged from 88% to 95%, among which the T-ALL patients had a risk of CNS leukemia, which was 3-4 times higher compared with B-precursor ALL patients. CONCLUSIONS These results indicate that long-term intrathecal CNS prophylaxis as well as appropriate systemic therapy for the non-high-risk patients can provide protection against CNS relapse equivalent to that provided by cranial irradiation.
The effects of in ovo injection of glucose and magnesium in broiler breeder eggs on hatching traits , performance , carcass characteristics and blood parameters of broiler chickens

Glucose (C6H12O6, also known as D-glucose, dextrose, or grape sugar) is a simple sugar (monosaccharide) and an important carbohydrate. Cells use it as the primary source of energy and as a metabolic intermediate (CLARK and SOKOLOF, 1999). It is used as an energy source in most organisms, from bacteria to humans (STRYER, 1995). Nevertheless, the whole carbohydrate of the chicken egg doesn’t exceed 1%. Despite the small amount of carbohydrate supply, glucose is the most important source of energy needed for the growth of the chicken embryo (STARCK and RICKELEFS, 1998). Therefore, the glucose naturally available in eggs may not be sufficient to meet the immediate metabolic demands of the embryo. The survey of all chicken egg carbohydrates shows that the amount of the chicken egg carbohydrate achieves its least amount until the 7th day of incubation of the embryo (DONHOFFER, 1933). Overall, the organogenesis of important segments of the chicken embryo occurs in the first week of incubation. At 4–7 days of incubation the gastro-intestinal organogenesis includes foregut, midgut and hindgut differentiations. On the other side, formation of most important organs including ovary, ileum, femur, pancreas, gastrocnemius muscle, duodenum and etc takes place at day 9 of incubation. Due to these evidences, in ovo injection of essential energy supplemental nutrients in this critical period (day-7) can be an efficient stimulator for optimal growth and development of organs (BELLAIRS and OSMOND, 2005). In the past studies on “in ovo feeding”, almost all of the works were conducted at days 17–21, the late-embryonic or pre-hatch stages (UNI et al., 2005; FOYE et al., 2007; ZHAI et al., 2008; CHEN et al., 2009; KERALAPURATH et al., 2010; ZHAI et al., 2011). The novel point of the present study was to evaluate the effects of in ovo feeding in early-embryonic life, especially by in ovo injection of maternal nutrient supplements that can be useful during the whole embryonic life of the bird (until hatching). Also, chicken embryo prefers to use glucose rather
Ultrastructure of N‐diethylnitrosamine induced tumours in the nasal olfactory region of the Syrian golden hamster

Tumours of the nasal olfactory region induced in five Syrian golden hamsters ater s.c. treatment with N-diethylnitrosamine (DEN) were examined electron microscopically. The neoplasms consisted of two cell types, the ultrastructure of which showed them to have originated from the precursors of olfactory sensory cells (neuroblasts) and the precursors of sustentacular cells (basal cells).
Virtual Synchronous Power Strategy for Multiple HVDC Interconnections of Multi-Area AGC Power Systems

Automatic generation control (AGC) in multi-area interconnected power systems is experiencing several adaptions due to increasing level of power converter based components in the system. The concept of virtual synchronous power (VSP) to simulate the dynamic effects of virtual inertia emulations by HVDC links for higher level control applications is introduced and reflected in the multi-area AGC model. By using this proposed combination in the AGC model, the dynamic performance of the studied system shows a significant improvement. The proposed formulation is generalized for multi-area systems with multiple HVDC links. The active power loop control in VSP-based HVDC links has a second-order characteristic, which makes a simultaneous enabling of damping and inertia emulations into the system. Trajectory sensitivities are also used to analyze the effects of VSP's parameters on the system stability. The effectiveness of the proposed concept on dynamic improvements is tested through MATLAB simulation of a four-area system.
Application of Differential Scanning Calorimetry to the Characterization of Biopolymers

Generally, polymers can be classified according to their thermal and mechanical properties into thermoplastics, thermosets and elastomers. Thermoplastics are amorphous or semicrystalline polymers that soft or melt during heating and solidify during cooling. The heating/cooling/heating process can be repeated without perceptible changes in thermal and mechanical properties of thermoplastics. Thermosets during heating undergo chemical changes and this process is irreversible. Elastomers can be vulcanized (cross-linked under assistance of heat, light, or special chemicals like sulfur, peroxides) that makes them reversibly stretchable for small deformations but vulcanization is the irreversible process.
Cognitive Aspects Of Annual Reports - Field Independence-Dependence

The annual report is a communication instrument which may be used to differentiate among the various organizations in the business community. Both the results of economic activity (content), and the manner in which those results are presented (form),' constitute the message of the annual report (Li [1]). Owing to the potential impact of the annual report on decision makers, the Securities and Exchange Commission and the American Institute of Certified Public Accountants,2 through their institutional pronouncements, act to develop user confidence in the information contained in the annual report. These pronouncements have in large measure been addressed to monitoring content, leaving the presentation format of the annual report a discretionary variable. ' Any attempts to influence annual report user (stockholders, security
Substrate Specificity and Domain Functions of Extracellular Heparan Sulfate 6-O-Endosulfatases, QSulf1 and QSulf2*

The extracellular sulfatases (Sulfs) are an evolutionally conserved family of heparan sulfate (HS)-specific 6-O-endosulfatases. These enzymes remodel the 6-O-sulfation of cell surface HS chains to promote Wnt signaling and inhibit growth factor signaling for embryonic tissue patterning and control of tumor growth. In this study we demonstrate that the avian HS endosulfatases, QSulf1 and QSulf2, exhibit the same substrate specificity toward a subset of trisulfated disaccharides internal to HS chains. Further, we show that both QSulfs associate exclusively with cell membrane and are enzymatically active on the cell surface to desulfate both cell surface and cell matrix HS. Mutagenesis studies reveal that conserved amino acid regions in the hydrophilic domains of QSulf1 and QSulf2 have multiple functions, to anchor Sulf to the cell surface, bind to HS substrates, and to mediate HS 6-O-endosulfatase enzymatic activity. Results of our current studies establish the hydrophilic domain (HD) of Sulf enzymes as an essential multifunctional domain for their unique endosulfatase activities and also demonstrate the extracellular activity of Sulfs for desulfation of cell surface and cell matrix HS in the control of extracellular signaling for embryonic development and tumor progression.