Miracle-dz/UniFAR-FTUs huggingface dataset:
In vitro comparative analysis of two resonance frequency measurement devices: Osstell implant stability coefficient and Penguin resonance frequency analysis.

Adequate implant stability is an essential requirement. The introduction of the Penguin resonance frequency analysis raises some questions regarding its reliability, reproducibility, and repeatability as well as how it compares to the older Osstell device. PURPOSE To assess the newer Penguin implant stability coefficient (ISQ) device (vs the Osstell device). MATERIALS AND METHODS A total of 120 implants were used, divided into four groups (A, B, C, and D) (according to design) and placed in fresh bovine bone. Consecutive measurements were made with both devices (Penguin/Osstell) with their respective transducers. Then, the ISQ values were measured with the Penguin device using the Osstell transducer, and vice-versa. RESULTS The mean insertion torque (N/cm) values for the implants were as follows: Group A = 24.7 ± 9.4; Group B = 25.6 ± 9.7; Group C = 28.7 ± 7.9; Group D = 19.1 ± 5.5. The mean ISQ values for the entire sample were as follows: Penguin 67.7 ± 6.1 and Osstell 68.5 ± 9.6. The ISQ value measured with the Penguin device using a SmartPeg transducer was 67.0 ± 8.0, and that for the Osstell device using a MultiPeg transducer was 68.3 ± 7.5. The intraclass correlation coefficient (ICC) was calculated for the ISQ values obtained from both devices and was >0.90 for all transducers. When the ICC transducers were interchanged, the values were <0.77. CONCLUSIONS Both ISQ devices allow for reliable and repeatable measurement of implant stability; however, the use of each device-specific transducer is recommended.
Hsa-miR-587 Regulates TGFβ/SMAD Signaling and Promotes Cell Cycle Progression

Objective Transforming growth factor beta/single mothers against decapentaplegic (TGFβ/SMAD) signaling pathway plays important roles in various biological processes. It acts as a tumor suppressor during the early stages of cancer progression. Discovering the regulators of this pathway provides important options for therapeutic strategies. Here, we searched for candidate microRNAs (miRNAs) that potentially target the critical components of the TGFβ signaling pathway. Materials and Methods In the current experimental study, we first predicted miRNAs that target TGFβ components using a bioinformatics software. After that, quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-587, TGFBR2, SMAD4, p21, CCND1 and c-MYC genes in transfected HEK293T and HCT116 cells. Dual Luciferase assay was performed to analyze the interactions between miRNAs and the target genes. Propidium iodide flow cytometry was used to determine cell cycle progression in HEK293T and HCT116 cells under hsa-miR-587 (miR-587) overexpression circumstances. Results Multiple miRNA responsive elements (MREs) were predicted for miR-587 within the 3’UTRs of the TGFBR2 and SMAD4 genes. Overexpression of miR-587 in HEK293T and HCT116 cells resulted in downregulation of TGFBR2 and SMAD4 genes. In addition, a downstream target gene of TGFβ/SMAD signaling, P21, was significantly downregulated in the HCT116 cells overexpressing miR-587. Dual luciferase assay analysis provided evidence that there is a direct interaction between miR-587 and the 3’UTR sequences of TGFBR2 and SMAD4 genes. Moreover, miR-587 overexpression in HEK293T and HCT116 cells resulted in reducing the SubG1 cell populations in both cell lines, as detected by flow cytometry. Conclusion Altogether, our data revealed an important role for miR-587 in regulating TGFβ/SMAD signaling and promoting cell cycle progression. These characteristics suggest that miR-587 is an important candidate for cancer therapy research.
The power of cooperation: Experimental and computational approaches in the functional characterization of bacterial sRNAs

Trans‐acting small regulatory RNAs (sRNAs) are key players in the regulation of gene expression in bacteria. There are hundreds of different sRNAs in a typical bacterium, which in contrast to eukaryotic microRNAs are more heterogeneous in length, sequence composition, and secondary structure. The vast majority of sRNAs function post‐transcriptionally by binding to other RNAs (mRNAs, sRNAs) through rather short regions of imperfect sequence complementarity. Besides, every single sRNA may interact with dozens of different target RNAs and impact gene expression either negatively or positively. These facts contributed to the view that the entirety of the regulatory targets of a given sRNA, its targetome, is challenging to identify. However, recent developments show that a more comprehensive sRNAs targetome can be achieved through the combination of experimental and computational approaches. Here, we give a short introduction into these methods followed by a description of two sRNAs, RyhB, and RsaA, to illustrate the particular strengths and weaknesses of these approaches in more details. RyhB is an sRNA involved in iron homeostasis in Enterobacteriaceae, while RsaA is a modulator of virulence in Staphylococcus aureus. Using such a combined strategy, a better appreciation of the sRNA‐dependent regulatory networks is now attainable.
Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS

Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis. Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools. Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski. We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS. Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively. The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively. Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties. In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1). These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.
Sublamina‐specific organization of the blood brain barrier in the mouse olfactory nerve layer

Astrocytes constitute the main glial component of the mammalian blood brain barrier (BBB). However, in the olfactory bulb (OB), the olfactory nerve layer (ONL) is almost devoid of astrocytes, raising the question which glial cells are part of the BBB. We used mice expressing EGFP in astrocytes and tdTomato in olfactory ensheathing cells (OECs), a specialized type of glial cells in the ONL, to unequivocally identify both glial cell types and investigate their contribution to the BBB in the olfactory bulb. OECs were located exclusively in the ONL, while somata of astrocytes were located in deeper layers and extended processes in the inner sublamina of the ONL. These processes surrounded blood vessels and contained aquaporin‐4, an astrocytic protein enriched at the BBB. In the outer sublamina of the ONL, in contrast, blood vessels were surrounded by aquaporin‐4‐negative processes of OECs. Transcardial perfusion of blood vessels with lanthanum and subsequent visualization by electron microscopy showed that blood vessels enwrapped by OECs possessed intact tight junctions. In acute olfactory bulb preparations, injection of fluorescent glucose 6‐NBDG into blood vessels resulted in labeling of OECs, indicating glucose transport from the perivascular space into OECs. In addition, Ca2+ transients in OECs in the outer sublamina evoked vasoconstriction, whereas Ca2+ signaling in OECs of the inner sublamina had no effect on adjacent blood vessels. Our results demonstrate that the BBB in the inner sublamina of the ONL contains astrocytes, while in the outer ONL OECs are part of the BBB.
Estimating Premorbid Ability in Rehabilitation Patients Using the Test of Premorbid Functioning and Wide Range Achievement Test–Fourth Edition

Objective: This study examined premorbid ability estimate concordance using Test of Premorbid Functioning predicted Full Scale Intelligent Quotient (TOPF-IQ) and Wide Range Achievement Test–Fourth Edition Word Reading (WRAT4-WR). Method: The sample (N = 145) was 28% female with average age and education of 40.6 and 13.2 years, respectively. Outpatient neuropsychological evaluations were conducted in a rehabilitation setting. Measures included the TOPF, WRAT4-WR, Wechsler Adult Intelligence Scale–Fourth Edition, and other neuropsychological tests. Non-WAIS measures defined impairment groups. Analyses included t tests, pairwise correlations, concordance correlation coefficients, and root mean square differences. Results: TOPF-IQ, WRAT4-WR, and Full Scale Intelligent Quotient scores were not significantly different but were lower than normative mean. TOPF-IQ and WRAT4-WR showed acceptable agreement (concordance correlation coefficient = .92; root mean square difference = 5.9). Greater premorbid–current ability differences were observed in the impaired group. TOPF-IQ and WRAT4-WR showed lower but similar agreement with Full Scale Intelligence Quotient in the unimpaired group. Conclusions: Findings support the WRAT4-WR in predicting premorbid ability in rehabilitation settings.
Profile of Disease Related Deaths in Custodial Cases An Autopsy Based Experience

The death of a person in custody always raises suspicion of torture at the hands of authorities culminating in death. In view of above, the present study was conducted to bring to light the pattern of deaths among the prison population of northwest India and suggest suitable preventive measures. This study extended over a period of four years (January 2010 to December 2013) and analysed 60 such cases. Male predominance was observed. The maximum number of fatalities (26.6%) occurred in 21–30 years age group. Most (80%) of the prisoners belonged to rural areas. Contrary to the common perception, natural causes (80%) were responsible for death in the majority of cases, with lung pathologies (29.2%) as the major killer. The study concluded that provision of timely and adequate health care to prison inmates is likely to bring down the morbidity & mortality. It is likely to bring down the morbidity and mortality among prison inmates and also save the authorities from the unnecessary mental agony and harassment of getting accused of the death of the person.
Sleep-disordered breathing is associated with brain vascular reactivity in spinal cord injury

Objective To determine the population-level odds of individuals with spinal cord injury (SCI) experiencing fatigue and sleep apnea, to elucidate relationships with level and severity of injury, and to examine associations with abnormal cerebrovascular responsiveness. Methods We used population-level data, meta-analyses, and primary physiologic assessments to provide a large-scale integrated assessment of sleep-related complications after SCI. Population-level and meta-analyses included more than 60,000 able-bodied individuals and more than 1,800 individuals with SCI. Physiologic assessments were completed on a homogenous sample of individuals with cervical SCI and matched controls. We examined the prevalence of (1) self-reported chronic fatigue, (2) clinically identified sleep apnea, and 3) cerebrovascular responsiveness to changing CO2. Results Logistic regression revealed a 7-fold elevated odds of chronic fatigue after SCI (odds ratio [OR] 7.9, 95% confidence interval [CI] 3.5–16.2), and that fatigue and trouble sleeping are correlated with the level and severity of injury. We further show that those with SCI experience elevated risk of clinically defined sleep-disordered breathing in more than 600 individuals with SCI (pooled OR 3.1, 95% CI 1.3–7.5). We confirmed that individuals with SCI experience a high rate of clinically defined sleep apnea using primary polysomnography assessments. We then provide evidence using syndromic analysis that sleep-disordered breathing is a factor strongly associated with impaired cerebrovascular responsiveness to CO2 in patients with SCI. Conclusions Individuals with SCI have an increased prevalence of sleep-disordered breathing, which may partially underpin their increased risk of stroke. There is thus a need to integrate sleep-related breathing examinations into routine care for individuals with SCI.
Social and Behavioral Predictors of Adolescents’ Positive Attitude towards Life and Self

Positive attitude is an important cognitive component of optimism. Although optimism has been widely studied in adolescents’ health, there is limited knowledge about social and behavioral determinants of their positive attitude. The aim of this study was to identify the main predictors of a positive attitude towards life and self in adolescence. Data were collected in 2010 from 2562 Polish adolescents, aged 15 to 17 years old, within the Health Behavior in School-Aged Children survey. A positive attitude was measured using the 4-item Positive Attitude Scale (PAS). Univariate analysis of variance was conducted and then hierarchical linear regression models adjusted for gender, age and family affluence were estimated. The mean PAS score was 13.25 (SD = 3.74), on the scale ranged 0–20. Eight out of 18 variables were included in the final model, which explained 25.1% of PAS variability. Communication in the family and with peers, as well as neighborhood social capital showed the strongest impact on positive attitude in adolescents. Physical activity, eating breakfast and school performance were also found to be important predictors. The results of the study highlight the need to include the development of interpersonal competences, promoting physical activity and supporting school performance, in adolescents’ mental health promotion programs, particularly in girls.
A botanical drug composed of three herbal materials attenuates the sensorimotor gating deficit and cognitive impairment induced by MK‐801 in mice

A botanical drug derived from the ethanolic extract composed of Clematis chinensis Osbeck (Ranunculaceae), Trichosanthes kirilowii Maximowicz (Cucurbitaceae) and Prunella vulgaris Linné (Lamiaceae) has been used to ameliorate rheumatoid arthritis as an ethical drug in Korea. In our study, we investigated the effect of this herbal complex extract (HCE) on schizophrenia‐like behaviours induced by MK‐801.
Impaired Right Ventricular-Vascular Coupling in Young Adults Born Preterm.

1University of Wisconsin-Madison, Department of Biomedical Engineering, Madison, WI, USA 2University of Wisconsin-Madison, Department of Medicine, Madison, WI, USA 3University of Wisconsin-Madison, Department of Pediatrics, Madison, WI, USA 4University of Wisconsin-Madison, Department of Medical Physics, Madison, WI, USA 5University of Wisconsin-Madison, Department of Radiology, Madison, WI, USA 6San José State University, Department of Biomedical Engineering, San José, CA, USA 7University Medical Center Groningen, Department of Pediatric Cardiology, Groningen, The Netherlands
Experimental evidence that physical activity affects the multivariate associations among muscle attachments (entheses)

ABSTRACT The morphology of entheses (muscle/tendon attachment sites) on bones is routinely used in paleontological and bioarcheological studies to infer the physical activity patterns of ancient vertebrate species including hominins. However, such inferences have often been disputed owing to limitations of the quantitative methods commonly employed and a lack of experimental evidence demonstrating direct effects of physical activity on entheseal morphology. Recently, we introduced a new and improved method of quantifying and analyzing entheseal morphology that involves repeatable three-dimensional measurements combined with multivariate statistics focused on associations among multiple entheses. Here, to assess the validity of our method for investigating variation in entheseal morphology related to physical activity patterns, we analyzed femora of growing turkeys that were experimentally exercised for 10 weeks on either an inclined or declined treadmill or served as controls (N=15 individuals, 5 per group). Our multivariate approach identified certain patterns involving three different entheses (associated with the gluteus primus, medial gastrocnemius, vastus medialis and adductor magnus muscles) that clearly differentiated controls from runners. Importantly, these differences were not observable when comparing groups within each of the three entheseal structures separately. Body mass was not correlated with the resulting multivariate patterns. These results provide the first experimental evidence that variation in physical activity patterns has a direct influence on entheseal morphology. Moreover, our findings highlight the promise of our newly developed quantitative methods for analyzing the morphology of entheses to reconstruct the behavior of extinct vertebrate species based on their skeletal remains. Summary: This study presents an experimental validation of a recently developed method of reconstructing physical activity based on multivariate analyses of muscle attachments on bone surfaces.
Dietary patterns and risk of systemic lupus erythematosus in women

Objective Dietary intake is a complex exposure and a potential risk factor for systemic lupus erythematosus (SLE) due to its impact on lipid and glucose metabolism, oxidative stress, and the intestinal microbiome. We aimed to test whether a prudent dietary pattern is associated with a lower risk of SLE, and whether a Western dietary pattern is associated with a higher risk of SLE. Methods We prospectively investigated two dietary patterns and SLE risk among women in the Nurses' Health Study (NHS, 1984–2014) and Nurses' Health Study II (NHSII, 1991–2015). Food frequency questionnaires were completed every four years. Congruent with prior work in NHS and NHSII, we derived two separate dietary patterns (prudent and Western) using principal component analysis within each cohort. Incident SLE was confirmed by the American College of Rheumatology's 1997 criteria. We estimated hazard ratios (HR) and 95% confidence intervals (CI) for SLE by dietary pattern quartiles using Cox models adjusted for time-varying covariates. Models were performed separately in each cohort and results were meta-analyzed. Stratified analyses tested the association of dietary patterns with anti-dsDNA positive SLE and anti-dsDNA negative SLE. Results We confirmed 82 NHS incident SLE cases and 98 NHSII SLE cases during 3,833,054 person-years of follow-up. A higher (healthier) prudent dietary pattern score was not associated with SLE risk (meta-analyzed HRQ4 versus Q1 0.84 [95% CI 0.51, 1.38]). Women with higher (less healthy) Western dietary pattern scores did not have a significantly increased risk for SLE (meta-analyzed HRQ4 versus Q1 1.35 [95% CI 0.77, 2.35]). Results were similar after further adjustment for body mass index. Incident anti-dsDNA positive SLE and anti-dsDNA negative SLE were not associated with either dietary pattern. Conclusion We did not observe a relationship between prudent or Western dietary pattern score and risk of SLE.
Endometrial Immune Dysfunction in Recurrent Pregnancy Loss

Recurrent pregnancy loss (RPL) represents an unresolved problem for contemporary gynecology and obstetrics. In fact, it is not only a relevant complication of pregnancy, but is also a significant reproductive disorder affecting around 5% of couples desiring a child. The current knowledge on RPL is largely incomplete, since nearly 50% of RPL cases are still classified as unexplained. Emerging evidence indicates that the endometrium is a key tissue involved in the correct immunologic dialogue between the mother and the conceptus, which is a condition essential for the proper establishment and maintenance of a successful pregnancy. The immunologic events occurring at the maternal–fetal interface within the endometrium in early pregnancy are extremely complex and involve a large array of immune cells and molecules with immunoregulatory properties. A growing body of experimental studies suggests that endometrial immune dysregulation could be responsible for several, if not many, cases of RPL of unknown origin. The present article reviews the major immunologic pathways, cells, and molecular determinants involved in the endometrial dysfunction observed with specific application to RPL.
Chemical, Thermo-Mechanical and Antimicrobial Properties of DBD Plasma Treated Disinfectant-Impregnated Wipes during Storage

Disinfectant-impregnated wipes are broadly used in hospitals, as an important approach for infection prevention and control. But their ageing performance has rarely been studied. Untreated and Dielectric Barrier Discharge (DBD) plasma pre-treated wiping materials made of nonwoven 100% polyester (W1), nonwoven 55% cellulose/45% polyester (W2) and woven cotton (W3) were impregnated with a quaternary ammonium compound solution (ADBAC) for 30 min, 3, 7, 15, and 30 days of storage time and characterized in term of chemical, thermo-mechanical and antimicrobial efficacy. X-ray photoelectron spectroscopy analysis on the plasma-treated polyester wipes demonstrates the incorporation of reactive oxygen species on the fiber surface. Laser scanning microscopy demonstrates the plasma etching effect in smoothing the surface of the cotton wipe reducing the adsorption of ADBAC. The result showed no significant changes in breaking force and elongation during storage for W1 and W2. However, plasma treatment affects W3 in weft direction reducing the force at break in water and ADBAC treated wipes. Dynamic mechanical analysis results show that ADBAC and plasma treatment have a significant influence in W1 and W3 viscoelastic properties improving the elastic response limiting the polymeric chains mobility and the non-elastic response due to the etching effect, respectively. Overall, the plasma pre-treatment of ADBAC-impregnated wipes is able to enhance the antimicrobial performance and the storage time of polyester-containing wipes.