Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of substrate concentration on dual‐species biofilm population densities of Klebsiella oxytoca and Burkholderia cepacia in porous media

The long‐term operation of bioremediation technologies relies on the success of the contaminant‐degrading microorganism(s) to compete for available resources with microorganisms already present in an aquifer or those that may contaminate a bioreactor. Though research has been performed studying the interaction of multiple species in batch and chemostat reactors, little work has been done looking at multi‐species interactions in environments that more closely resemble field‐scale applications. The research presented herein examined the interaction of Burkholderia cepacia PR1‐pTOM31c, an aerobic trichloroethylene (TCE)‐degrading bacterium, with Klebsiella oxytoca, a facultative bacterium, in a flow‐through porous media (PM) reactor. Growth characteristics and population distributions in PM were compared to previously reported values from batch and chemostat reactors. The faster growing organism in batch experiments (K. oxytoca) did not always have the greater population density in dual‐species PM experiments. The biofilm population distribution was influenced by substrate concentration, with B. cepacia having a greater dual‐species population density than K. oxytoca at a low (30 mg/L dissolved organic carbon [DOC]) substrate concentration and K. oxytoca having a greater population density at a high (700 mg/L DOC) substrate concentration. This change in species population distribution with change in substrate concentration, which was not observed in batch reactors, was also observed in chemostat reactors. Therefore, manipulation of substrate concentration enabled the control of species dominance to the advantage of the TCE degrading population in this dual‐species PM system and may provide a mechanism to enhance bioremediation scenarios involving TCE or other contaminants of concern. © 2005 Wiley Periodicals, Inc.
Chemical composition and nutritive value of lantana and sweet pepper seeds and nabak seed kernels.

UNLABELLED Chemical analysis was carried out on lantana (Lantana camara) and sweet pepper (Capsicum annuum L.) seeds and nabak (Zizyphus spina-christi) seed kernels. The proximate analysis (on dry weight basis) of sweet pepper seeds, lantana seeds, and nabak seed kernels showed the following composition: moisture 70.95%, 17.27%, and 4.22%; ash 4.88%, 1.81%, and 3.51%; fat 19.57%, 11.0%, and 30.19%; crude protein 19.28%, 6.3%, and 38.2%; and carbohydrate 56.3%, 80.9%, and 28.1%, respectively. For minerals, potassium was the most abundant element, followed by phosphorus and sodium. Also, zinc, iron, copper, and manganese were detected. Analysis of amino acids revealed that the first limiting amino acid was valine, for both lantana and sweet pepper seeds, but it was threonine for nabak seed kernels. Antinutritional compounds, including, phytic acid, trypsin inhibitor, and tannins, were detected in all seeds. Results of fatty acid compositions showed that the major fatty acid was oleic acid in both lantana (48.73%) and nabak oils (53.25%), but it was linoleic acid in sweet pepper oil (71.55%). Moreover, the degree of unsaturation of these oils was close to that of common vegetable oils. In all oils, there was absorbance in the ultraviolet (UV)-B and UV-C ranges with potential for use as broad spectrum UV protectants. It can be inferred that the seeds investigated are good sources of crude fat, crude protein, ash, carbohydrate, and some minerals. Furthermore, the oil extracts could be useful as edible oils and for industrial applications. PRACTICAL APPLICATION The nutritional composition of the investigated seeds suggested that they could be used to meet part of the nutritional requirements of animal feeds. Also, they could be regarded as good sources of food ingredients and as new sources of edible oils.
Adolescent Beliefs About the Acceptability of Dating Violence

This longitudinal study examined the interplay between teens’ beliefs about the acceptability of dating violence and dating violence perpetration. The final sample included 82 teens aged 14 to 17 years. Families were recruited from truancy courts and juvenile probation and victim services offices. Teens participated in a baseline and a follow-up assessment spaced 3 months apart. At each assessment, teens reported on their beliefs about dating violence acceptability and their dating violence perpetration. Dating violence perpetration at baseline predicted beliefs accepting of violence at follow-up, after accounting for baseline levels of beliefs. Beliefs at baseline, however, did not predict dating violence perpetration at follow-up. Dating violence perpetration may lead to beliefs more accepting of such violence.
Efficient posterior exploration of a high‐dimensional groundwater model from two‐stage Markov chain Monte Carlo simulation and polynomial chaos expansion

This study reports on two strategies for accelerating posterior inference of a highly parameterized and CPU‐demanding groundwater flow model. Our method builds on previous stochastic collocation approaches, e.g., Marzouk and Xiu (2009) and Marzouk and Najm (2009), and uses generalized polynomial chaos (gPC) theory and dimensionality reduction to emulate the output of a large‐scale groundwater flow model. The resulting surrogate model is CPU efficient and serves to explore the posterior distribution at a much lower computational cost using two‐stage MCMC simulation. The case study reported in this paper demonstrates a two to five times speed‐up in sampling efficiency.
Placental epigenetic patterning of glucocorticoid response genes is associated with infant neurodevelopment.

AIM To determine associations between methylation of NR3C1, HSD11B2, FKBP5 and ADCYAP1R1 and newborn neurobehavioral outcomes. METHODS In 537 newborns, placental methylation was quantified using bisulfite pyrosequencing. Profiles of neurobehavior were derived via the Neonatal Intensive Care Unit Network Neurobehavioral Scales. Using exploratory factor analysis, the relationships between methylation factor scores and neurobehavioral profiles were examined. RESULTS Increased scores of the factor characterized by NR3C1 methylation were associated with membership in a reactive, poorly regulated profile (odds ratio: 1.47; 95% CI: 1.00-2.18), while increased scores of the factor characterized by HSD11B2 methylation reduced this risk. CONCLUSION These results suggest that coordinated regulation of these genes influences neurobehavior and demonstrates the importance of examining these alterations in a harmonized fashion.
Pharmacokinetic interaction between efavirenz and dual protease inhibitors in healthy volunteers

The combination of efavirenz with HIV‐1 protease inhibitors (PI) results in complex interactions secondary to mixed induction and inhibition of oxidative metabolism. ACTG A5043 was a prospective, open‐label, controlled, two‐period, multiple‐dose study with 55 healthy volunteers. The objective of the present study was to evaluate the potential pharmacokinetic interaction between efavirenz and dual PIs. The subjects received a daily dose of 600 mg efavirenz for 10 days with amprenavir 600 mg twice daily added at day 11 and were randomized to receive nelfinavir, indinavir, ritonavir, saquinavir, or no second PI on days 15–21. Intensive pharmacokinetic studies were conducted on day 14 and 21. Efavirenz plasma concentrations were fit to candidate models using weighted non‐linear regression. The disposition of efavirenz was described by a linear two‐compartment model with first order absorption following a fitted lag time. Apparent clearance (CLt/F), volume of distribution at steady state (Vss/F), inter‐compartmental clearance, and the central and peripheral volume of distribution were estimated. The mean CLt/F and Vss/F of efavirenz were 0.126 l/h/kg and 4.412 l/kg, respectively. Both AUC and CLt/F of efavirenz remained unchanged after 7 days of dual PI dosing. The mean Vss/F of efavirenz increased an average of 89% across arms, ranging from 52% (nelfinavir) to 115% (indinavir) relative to efavirenz with amprenavir alone. Increases were also observed in Vp/F after the addition of nelfinavir, indinavir, ritonavir and saquinavir by 85%, 170%, 162% and 111%, respectively. In conclusion, concomitant administration of dual PIs is unlikely to have any clinically significant effect on the pharmacokinetics of CYP2B6 substrates in general or oral efavirenz specifically. Copyright © 2007 John Wiley & Sons, Ltd.
The Epidemiology of Nonalcoholic Fatty Liver Disease in Adults

Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disease that has been shown to progress to cirrhosis and hepatocellular carcinoma. This article reviews the prevalence of NAFLD and the factors associated this disorder, and with the more advanced stages of NAFLD, including nonalcoholic steatohepatitis (NASH) and fibrosis. In the general population, the estimated prevalence ranges from 3% to 24%, with most estimates in the 6% to 14% range. NAFLD is extremely common among patients undergoing bariatric surgery, ranging from 84% to 96%. In these patients, 25% to 55% have NASH, 34% to 47% have fibrosis, and 2% to 12% have bridging fibrosis or cirrhosis. NAFLD appears to be most strongly associated with obesity, and insulin resistance states including diabetes and with other features of the metabolic syndrome, such as high triglycerides and low HDL. It appears to be more common in men, and it increases with increasing age and after menopause. Some data suggest that Mexican Americans are more likely to have NAFLD and blacks are less likely compared with non-Hispanic whites. More advanced stages of NAFLD are associated with older age, higher body mass index, diabetes, hypertension, high triglycerides, and/or insulin resistance. An AST/ALT ratio greater >1 may also indicate more severe disease. Although hepatocellular carcinoma can occur in the setting of NAFLD, the risk factors for hepatocellular carcinoma in the setting of NAFLD have not been established. More prospective studies are needed to determine the true risk factors for the development and progression of NAFLD to help identify patients at highest risk who might benefit from treatment trials.
Pharmacology of acute lung injury.

The acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a clinical syndrome that affects both medical and surgical patients. To date, despite improved understanding of the pathogenesis of ALI/ARDS, pharmacological modalities have been unsuccessful in decreasing mortality. However, several pharmacological agents for ARDS are in development and have shown great promise. In addition to the anti-inflammatory category including late corticosteroids, inhaled nitric oxide, alveolar surfactant, and vasodilators are being evaluated. Replacements of anticoagulation mediators have also suggested beneficial effects on the patient outcome. This article provides an overview of pharmacological treatments of ALI/ARDS.
The activities of endothelial elastase and cathepsin G in rats at oxidative stress caused by heavy metals salts

CoCl2 introduction increased cathepsin G activity in the heart and liver as well as endothelial elastase (EEl) in kidney that indicated the development of destructive processes. CoCl2 introduction decreased EEl and cathepsin G activities in blood serum and cathepsin G in lungs. HgCl2 injection decreased EEl in blood serum, heart, liver, kidney and cathepsin G in blood serum. These decreasing of proteinases activities may be caused by cytotoxic effects of heavy metals and/or the inclusion of these proteases in the destructive processes and absence of their synthesis and/or release.
Sur la théorie élémentaire des corps de fonctions

Abstract We study the first order theory of function fields in the language of fields by using fundamental results on curves in algebraic geometry. We give some applications; for example, using a theorem of G. Cherlin, we prove the undecidability of function fields with nonzero characteristic over an algebraically closed field.
Conservation of the 15-kilodalton lipoprotein among Treponema pallidum subspecies and strains and other pathogenic treponemes: genetic and antigenic analyses

The 15-kDa lipoprotein of Treponema pallidum is a major immunogen during natural syphilis infection in humans and experimental infection in other hosts. The humoral and cellular immune responses to this molecule appear late in infection as resistance to reinfection is developing. One therefore might hypothesize that this antigen is important for protective immunity. This possibility is explored by using both genetic and antigenic approaches. Limited or no cross-protection has been demonstrated between the T. pallidum subspecies and strains or between Treponema species. We therefore hypothesized that if the 15-kDa antigen was of major importance in protective immunity, it might be a likely site of antigenic diversity. To explore this possibility, the sequences of the open reading frames of the 15-kDa gene have been determined for Treponema pallidum subsp. pallidum (Nichols and Bal-3 strains), T. pallidum subsp. pertenue (Gauthier strain), T. pallidum subsp. endemicum (Bosnia strain), Treponema paraluiscuniculi (Cuniculi A, H, and K strains), and a little-characterized simian isolate of Treponema sp. (Fribourg-Blanc strain). No significant differences in DNA sequences of the genes for the coding region of the 15-kDa antigen were found among the different species and subspecies studied. In addition, all organisms showed expression of the 15-kDa antigen as determined by monoclonal antibody staining. The role of the 15-kDa antigen in protection against homologous infection with T. pallidum subsp. pallidum Nichols was examined in rabbits immunized with a purified recombinant 15-kDa fusion protein. No alteration in chancre development was observed in immunized, compared to unimmunized, rabbits, and the antisera induced by the immunization failed to enhance phagocytosis of T. pallidum subsp. pallidum by macrophages in vitro. These results do not support a major role for this antigen in protection against syphilis infection.
A Herschel view of the far-infrared properties of submillimetre galaxies

We study a sample of 61submillimetre galaxies (SMGs) selected from ground-based surveys, with known spectroscopic redshifts and observed with the Herschel Space Observatory as part of the PACS Evolutionary Probe (PEP) and the Herschel Multi-tiered Extragalactic Survey (HerMES) guaranteed time key programmes. Our study makes use of the broad far-infrared and submillimetre wavelength coverage (100−600 μm) only made possible by the combination of observations from the PACS and SPIRE instruments aboard the Herschel Space Observatory. Using a power-law temperature distribution model to derive infrared luminosities and dust temperatures, we measure a dust emissivity spectral index for SMGs of β = 2.0 ± 0.2. Our results unambiguously unveil the diversity of the SMG population. Some SMGs exhibit extreme infrared luminosities of ~10^(13) L_⊙ and relatively warm dust components, while others are fainter (a few times 10^(12) L_⊙) and are biased towards cold dust temperatures. Although at z~2 classical SMGs (>5 mJy at 850 μm) have large infrared luminosities (~10^(13) L_⊙ ), objects only selected on their submm flux densities (without any redshift informations) probe a large range in dust temperatures and infrared luminosities. The extreme infrared luminosities of some SMGs (L_IR ≳ 10^(12.7) L_⊙, 26/61 systems) imply star formation rates (SFRs) of >500 M_⊙ yr^(-1) (assuming a Chabrier IMF and no dominant AGN contribution to the FIR luminosity). Such high SFRs are difficult to reconcile with a secular mode of star formation, and may instead correspond to a merger-driven stage in the evolution of these galaxies. Another observational argument in favour of this scenario is the presence of dust temperatures warmer than that of SMGs of lower luminosities (~40 K as opposed to ~25 K), consistent with observations of local ultra-luminous infrared galaxies triggered by major mergers and with results from hydrodynamic simulations of major mergers combined with radiative transfer calculations. Moreover, we find that luminous SMGs are systematically offset from normal star-forming galaxies in the stellar mass-SFR plane, suggesting that they are undergoing starburst events with short duty cycles, compatible with the major merger scenario. On the other hand, a significant fraction of the low infrared luminosity SMGs have cold dust temperatures, are located close to the main sequence of star formation, and therefore might be evolving through a secular mode of star formation. However, the properties of this latter population, especially their dust temperature, should be treated with caution because at these luminosities SMGs are not a representative sample of the entire star-forming galaxy population.
Free postsynaptic densities in the hippocampus of the female rat

THE number of synapses in the adult, female hippo-campal CA1 region fluctuates naturally across the estrous cycle in an ovarian steroid-dependent manner. This phasic variation in synapse number occurs without identifiable degenerating synapses. Ultrastructural correlates of the dynamic aspect of this synapse loss and synapse formation thus remain undescribed. During early development, one hallmark of synaptogenesis is the presence of free postsynaptic densities (PSDs). Here we report that the incidence of free PSDs in CA1 fluctuates across the rat estrous cycle. The number of free PSDs is greatest on the afternoon of proestrus and is significantly decreased on the afternoon of estrus, 24 h later. We hypothesize that these free PSDs reflect synapse turnover in the adult CA1 region.
Profile of morbidity among elderly at home health care service in Southern Saudi Arabia

Background: This study aimed to determine the profile of morbidity among elderly registered at home health care service in the Armed Forces Hospital of Southern Region, Kingdom of Saudi Arabia. Materials and Methods: A retrospective study was conducted (over a period of 6 months during year 2011) and data was collected by reviewing of medical records of all elderly patients of elderly. Results: The total number of elderly ≥ 60 years were 880. The most prevalent morbidity is hypertension (59.1%) followed by diabetes mellitus (57.3%), stroke (34.9%), dementia (28.5%), osteoarthritis (24.2%) and Alzheimer (21.4%). Females are at higher risks of having many types elderly diseases compared to males. The highest risk was for obesity (OR = 9.1; 95% CI = 3.51- 12.8), followed by osteoporosis (OR = 8.7; 95% CI = 15.10 – 9.13) and fracture neck femur (OR = 3.9; 95 CI = 2.11 – 6.91). In addition, females were also at higher risks of having Osteoarthritis and thyroid disorder. On the other hand, males are more susceptible to hypertension (OR = 1.4; 95 % CI = 1.07 – 1.85), stroke (OR = 1.3; 95 % CI = 1.08 – 1.89) and renal diseases (OR = 2.4; 95% CI = 1.25 – 4.54). Conclusion: It is concluded that there is a great need for preventive, curative and rehabilitative program in order to introduce high quality of health care services to elderly.
Sheared deep vortical convection in pre‐depression Hagupit during TCS08

Airborne Doppler radar observations from the recent Tropical Cyclone Structure 2008 field campaign in the western North Pacific reveal the presence of deep, buoyant and vortical convective features within a vertically‐sheared, westward‐moving pre‐depression disturbance that later developed into Typhoon Hagupit. On two consecutive days, the observations document tilted, vertically coherent precipitation, vorticity, and updraft structures in response to the complex shearing flows impinging on and occurring within the disturbance near 18 north latitude. The observations and analyses herein suggest that the low‐level circulation of the pre‐depression disturbance was enhanced by the coupling of the low‐level vorticity and convergence in these deep convective structures on the meso‐gamma scale, consistent with recent idealized studies using cloud‐representing numerical weather prediction models. Further examination of these new observations is needed to quantify the relative role of these vortical convection features in the tropical cyclone spin up process.
Cell surface-bound IL-1α is an upstream regulator of the senescence-associated IL-6/IL-8 cytokine network

Inflammation underlies most age-related diseases, including cancer, but the etiology is poorly understood. One proposed factor is the presence of senescent cells, which increase with age. The senescence response arrests the proliferation of potentially oncogenic cells, and most senescent cells secrete high levels of proinflammatory cytokines and other proteins. The complex senescence-associated secretory phenotype is likely regulated at multiple levels, most of which are unknown. We show that cell surface-bound IL-1α is essential for signaling the senescence-associated secretion of IL-6 and IL-8, 2 proinflammatory cytokines that also reinforce the senescence growth arrest. Senescent human fibroblasts expressed high levels of IL-1α mRNA, intracellular protein, and cell surface-associated protein, but secreted very little protein. An IL-1 receptor (IL1R) antagonist, neutralizing IL-1α antibodies, and IL-1α depletion by RNA interference all markedly reduced senescence-associated IL-6/IL-8 secretion. Depletion of the key IL-1R signaling component IRAK1 also suppressed this secretion, and IL-1α neutralizing antibodies prevented IRAK1 degradation, indicating engagement of the IL-1R signaling pathway. Furthermore, IL-1α depletion reduced the DNA binding activity of NF-κB and C/EBPβ, which stimulate IL-6/IL-8 transcription. IL-1α was a general regulator of senescence-associated IL-6/IL-8 secretion because IL-1α blockade reduced IL-6/IL-8 secretion whether cells senesced owing to DNA damage, replicative exhaustion, oncogenic RAS, or chromatin relaxation. Furthermore, conditioned medium from IL-1α-depleted senescent cells markedly reduced the IL-6/IL-8-dependent invasiveness of metastatic cancer cells, indicating that IL-1α regulates the biological effects of these cytokines. Thus, cell surface IL-1α is an essential cell-autonomous regulator of the senescence-associated IL-6/IL-8 cytokine network.
A free boundary problem in biological selection models

We prove local existence for classical solutions of a free boundary problem which arises in one of the biological selection models proposed by Brunet and Derrida, [3]. The problem we consider describes the limit evolution of branching brownian particles on the line with death of the leftmost particle at each creation time as studied in [7]. We use extensively results in [1] and [2].