Miracle-dz/UniFAR-FTUs huggingface dataset:
Ber‐EP4 antigen is a marker for a cell population related to the secondary hair germ

Abstract: Ber‐EP4 is an antibody to a cell membrane glycoprotein of unknown function. In the skin, Ber‐EP4 immunoreactivity has been reported to be localized in structures composed of basaloid epithelial cells, i.e. fetal epithelial germ cells, basal cell carcinoma, and trichoepithelioma as well as eccrine or apocrine ducts. In this study, we further characterized the follicular expression of Ber‐EP4 immunoreactivity at different stages of the hair cycle of human terminal hair follicles. In addition, to clarify the location of Ber‐EP4+ cells, we compared the Ber‐EP4 immunoreactivity with the expression of keratin 15 and keratin 19. Positive staining by Ber‐EP4 was found in the lower part of the epithelial strand of late catagen hair follicles, in the secondary hair germ of telogen hair follicles, and in the matrix of early anagen hair follicles but not in any parts of mature anagen hair follicles. In contrast, the follicular expression of keratin 15 detected by using LHK15 antibody was restricted to two distinct parts of anagen hair follicles, i.e. the outer root sheath above the hair bulb and that of the isthmus including the bulge area, and to the outer root sheath of late catagen and telogen hair follicles. The follicular expressions of keratin 19 and that of keratin 15 were apparently superimposed, whereas keratin 15 expression was more extended. The immunoreactivity of LHK15 antibody and antikeratin 19 antibody against the secondary hair germ of telogen follicles was negative or dim. Our results suggest that Ber‐EP4 reacts with the secondary hair germ and possibly a cell population related to the secondary hair germ but not with the presumptive stem cell population as revealed immunohistochemically either by the keratin 15 or keratin 19 expression.
EFFECT OF BEE POLLEN AT DIFFERENT LEVELS AS NATURAL ADDITIVES ON IMMUNITY AND PRODUCTIVE PERFORMANCE IN RABBIT MALES

This study was designed to evaluate the effect of using bee pollen (BP) at different levels as a natural growth promoter on the productive performance, digestibility, carcass traits, caecal microbial activity and some blood parameters of growing rabbits. Sixty New Zealand White (NZW) male rabbits, 5 weeks old were randomly divided into four equal groups, with 695.75± 10.01 g BW. In the 1 group, rabbits were fed without any supplementation and considered as control group. While, those in the 2, 3, and 4 groups were daily treated with BP at 200, 500 and 700 mg/kg BW, respectively and served as low, medium and high treatment levels. From these findings could be noted that the growing rabbits treated with BP significantly (P≤0.05) increased final body weight and total weight gain and decreased feed intake (DM) as well as improved in feed conversion as compared with control group. Both of digestibility coefficients and nutritive values of treated rabbits were significant (P≤0.05) improved than that of the control. The dressing weight, carcass weight and kidney fat were significantly (P≤0.05) increased with increasing BP levels. The immunoglobulin concentration (total antioxidants capacity, IgG, IgM and IgA) increased significantly (P≤0.05) with increasing BP levels. The amylase, lipase, and protease activities were significantly (P≤0.05) increased in the pancreatic homogenate and intestinal contents in the rabbits treated with high levels of BP as compared to control group. The best economical efficiency was obtained at the high BP level compared to other treatment groups. Conclusively, from the obtained results it could be concluded that the supplementation of BP at any levels studied had positive effects on rabbit’s performance, blood lipid regulation which reflecting their antioxidant of growing rabbits.
Unraveling the functions of plasmacytoid dendritic cells during viral infections, autoimmunity, and tolerance

Summary: Plasmacytoid dendritic cells (pDCs) are bone marrow‐derived cells that secrete large amounts of type I interferon (IFN) in response to viruses. Type I IFNs are pleiotropic cytokines with antiviral activity that also enhance innate and adaptive immune responses. Viruses trigger activation of pDCs and type I IFN responses mainly through the Toll‐like receptor pathway. However, a variety of activating and inhibitory pDC receptors fine tune the amplitude of type I IFN responses. Chronic activation and secretion of type I IFN in the absence of infection can promote autoimmune diseases. Furthermore, while activated pDCs promote immunity and autoimmunity, resting or alternatively activated pDCs may be tolerogenic. The various roles of pDCs have been extensively studied in vitro and in vivo with depleting antibodies. However, depleting antibodies cross‐react with other cell types that are critical for eliciting protective immunity, potentially yielding ambiguous phenotypes. Here we discuss new approaches to assess pDC functions in vivo and provide preliminary data on their potential roles during viral infections. Such approaches would also prove useful in the more specific evaluation of how pDCs mediate tolerance and autoimmunity. Finally, we discuss the emergent role of pDCs and one of their receptors, tetherin, in human immunodeficiency virus pathogenesis.
Phase II trial of nab‐paclitaxel in patients with relapsed or refractory multiple myeloma

between 0.1% and 0.72%. There was a weak significant negative Spearman correlation between the percentage of these cells and age (r 5 20.232 P 5 0.022). We could not detect significant correlations between hematogones and the IPSS-R score, the number of aberrancies in the granulocytic lineage, or percentage of myeloid progenitors, but there were significant negative correlations with the number of monocytic aberrancies (r 5 20.256; P 5 0.012), the number of abnormalities of CD34 cells (r 5 20.206; P 5 0.044), and the total number of aberrancies detected (r 5 20.334; P 5 0.0008). In the univariate Cox regression, the percentage of B-cell precursors was a significant favorable prognostic factor for overall survival (B 5 26.220; P 5 0.011). In the Kaplan–Meier curve, patients with detectable B-cell precursors in BM had a significant better overall survival (log-rank test: P< 0.001) than patients with undetectable CD34/CD19/CD10 cells. After 5 years, 76.7% of the patients presenting BM hematogones at diagnosis were still alive, whereas this value dropped to 51.9% in patients without detectable B-cell precursors (Fig. 1). In a multivariate Cox regression model, the percentage of hematogones (B 5 25.061; P 50.006), of myeloid progenitors (B 5 0.191; P 50.02), and the IPSS-R score (B 5 0.714; P< 0.001) remained in the final model, thus demonstrating that the percentage of B-cell precursors is an independent favorable prognostic factor in MDS patients. In order to estimate the clinical importance of the variable “hematogones” in the model, we calculated the relative Akaike weights (w) for different models according to the Akaike information criteria. The relative weight for the prognostic model based only on IPSS-R was 0.0179 and raised to 0.112 in a model containing IPSS-R and the percentage of myeloid precursors. The weight was even higher in a model based on IPSS-R and the percentage of B-cell precursors (w 5 0.195), but the best model was achieved joining the variables “percentage of B-cell precursors,” “myeloid progenitors,” and the “IPSS-R score” (w 5 0.675). These data indicate that the percentage of hematogones in BM at diagnosis adds important prognostic information independent from already well-known prognostic features for overall survival in MDS patients. This is in keeping with similar results found in the setting of hematological recovery after allogeneic BM transplantation, where the absence of B-cell precursors in BM on day 130 was associated with a higher non-relapse mortality, a higher frequency of acute graft-versus-host disease and infections [6]. In normal individuals, there is a decrease of B-cell precursors, especially after the age 55 years, but these cells are even more decreased in patients with MDS compared to age-matched persons [5]. All these data indicate that the number of B-cell precursors in BM is not only a good marker of a preserved immune function but also for the integrity of the hemopoietic stem cell.
Accidental overdosage of recombinant factor VIIa due to mistaken package size

A71-year-old male patient (156 cm, 50 kg) was admitted because of persistent macrohematuria due to a large tumor of the bladder. Medical history was clear, except for a mild form of COPD treated with 2.5 mg prednisolone and theophylline. Hemodynamics were stable on admission. Global coagulation parameters revealed a spontaneously prolonged aPTT of 47.2 sec (Table 1). Unfortunately, the patient did not report any previous bleeding episodes. Further coagulation studies were not ordered. Thus, after performing spinal anesthesia the tumor was partly resected and coagulated transurethral during an emergency operation. Bleeding ceased. On the morning of the first postoperative day, bleeding started again. Hemoglobin dropped from 12.3 to 9.2 g/dl within 4 hours. Further investigation was required. Global coagulation parameters were unchanged when compared to admission. Intraoperatively the patient received 4 RBC, 2 FFP and 1000 ml colloids. Bleeding was reduced, but did not cease despite repetitive transurethral coagulation and application of 0.5 g tranexamic acid. Thus, the patient was admitted to the ICU and continuous lavage of the bladder with iced water established. DDAVP at 0.4 μg/kg and additional tranexamic acid 1.0 g had no influence on bleeding. During the following 24 hours the patient’s condition deteriorated further. Due to continuous lavage of the bladder, he became hypothermic [core temperature 35.0 °C] despite warming with a forced air patient warming device. Because of continuous hemorrhage, hemodynamics became instable. Another 6 RBC, 10 FFP and 1500 ml colloids had been administered. Due to massive transfusion, coagulation status showed some typical results, for example, dilutional thrombocytopenia. Platelet count rose from 49 to 103 × 103/μl after transfusion of one aphaeresis concentrate. Continuous norepinephrine infusion at 0.8 mg/h was started. Because the hemorrhage was intractable by conventional means and the patient’s condition deteriorated further, we decided to use rFVIIa off-label as a last resort on the morning of the second day after admission. The patient received two identical boli of rFVIIa at 5:30 a.m. and 11:00 a.m. Bleeding ceased after the first injection and did not reoccur. The patient’s condition stabilized. Norepinephrine infusion was discontinued, hemoglobin stabilized without any further transfusion at about 10.0 g/dl. Despite this excellent outcome a severe mistake occurred with the application of rFVIIa. Dosage was calculated on a package size of 1.2 mg. So with a standard dose regimen of 90 μg/kg, the patient had to receive 4 packages of 1.2 mg each per bolus. Unfortunately, the actual application was done with packages containing 4.8 mg each; so the patient twice received 384 μg/kg instead of 90 μg/kg. Despite these two mega doses, Accidental overdosage of recombinant factor VIIa due to mistaken package size
Compliance with the Commission on Cancer Quality of Breast Cancer Care Measures: Self‐Evaluation Advised

Abstract: To provide evaluations of cancer care quality, the Commission on Cancer and the National Quality Forum (NQF) established three breast cancer treatment quality measures. Programs that submit data to the National Cancer Data Base (NCDB) can receive feedback on their compliance with these quality measures, and perform comparisons with other member institutions. Data received by a county hospital from the NCDB revealed poor compliance. The purpose of this study was to evaluate the accuracy of submitted data, identify contributing factors and initiate processes to improve. Reported 2004 NCDB quality measure compliance was 26% for radiation, 61.4% for chemotherapy, and 21.3% for hormonal therapy. Retrospective treatment review was performed. Data collected included: patient demographics, pathology, final surgical intervention, adjuvant treatment, and quality measure compliance. Sources included two electronic records, an electronic results depository, two paper charts, a pharmacy data base, and a “shadow chart. ” Applicability of and compliance with these quality measures was noted. Of 540 records reviewed, 132 met final study criteria. Actual compliance differed significantly from NCDB rates and were found to be 97% for radiation, 98% for chemotherapy, and 88% for hormonal therapy. Process analysis revealed the need for tumor registry staff to evaluate all sources of data. A significant problem was neo‐adjuvant chemotherapy and the requirement to submit NCDB data within 6 months of initial diagnosis. Processes and education initiated for tumor registry staff, medical records personnel, physicians, and other care providers resulted in significantly improved 2007 compliance of data submitted to the NCDB. Prior to public reporting, institutions should perform NQF quality measure compliance assessments, confirm accuracy, and initiate educational processes/imperatives.
Outer Membrane Protein Heterogeneity within Pseudomonas fluorescens and P. putida and Use of an OprF Antibody as a Probe for rRNA Homology Group I Pseudomonads

The electrophoretic patterns of outer membrane proteins of strains representing the biovars of Pseudomonas fluorescens and Pseudomonas putida were analyzed by gel electrophoresis. The outer membrane protein profiles were variable, and they were not useful for assigning strains to a specific biovar. However, three or four predominant outer membrane proteins migrating at 42 to 46 kDa, 33 to 38 kDa, and 20 to 22 kDa were conserved among the strains. They could be tentatively identified as OprE (44 kDa), OprF (38 kDa), OprH (21 kDa), and OprL (20.5 kDa), which are known proteins from Pseudomonas aeruginosa. A 37-kDa OprF-like protein was purified from P. fluorescens DF57 and used to raise a polyclonal antibody. In Western blot (immunoblot) analysis, this antibody reacted with OprF proteins from members of Pseudomonas rRNA homology group I but not with proteins from nonpseudomonads. The heterogeneity in M(infr) of OprF was greater among P. fluorescens strains than among P. putida strains. Immunofluorescence microscopy of intact cells demonstrated that the antibody recognized epitopes that were accessible only after unmasking by EDTA treatment. The antibody was used in a colony blotting assay to determine the percentage of rRNA homology group I pseudomonads among bacteria from the rhizosphere of barley. The bacteria were isolated on 10% tryptic soy agar, King's B agar, and the pseudomonad-specific medium Gould S1 agar. The estimate of OprF-containing CFU in rhizosphere soil obtained by colony blotting on 10% tryptic soy agar was about 2 and 14 times higher than the values obtained from King's agar and Gould S1 agar, respectively, indicating that not all fluorescent pseudomonads are scored on more specific media. The colonies reacting with the OprF antibody were verified as being rRNA homology group I pseudomonads by using the API 20NE system.
The effect of occupant protection by controlling airbag and seatbelt

Recent research revealed that greater proportion of heavier male occupants and lighter female occupants sustain serious injuries in frontal crash. The cause is thought that the frontal occupant restraint systems are designed to minimize the injury risk for 50th percentile occupant only, and its characteristics are not adjustable. In this study, vent hole area of the airbag and load limiter force of the seat belt were controlled according to occupant sizes, and the effect of occupant protection for different occupant sizes were evaluated. MADYMO 3D was used in this analysis, and evaluation was performed not only in the 5th, 50th and 95th percentile occupant size dummies, but also in the various physiques occupant size dummies. The various physiques occupant size dummies were created using the physique scaling application of MADYMO. As a result, all sizes of occupant dummies, from 5th to 95th percentile occupant dummies were evaluated, and injury severity of those occupant dummies is described. For the covering abstract see ITRD E825082.
A lipooligosaccharide-binding site on HepG2 cells similar to the gonococcal opacity-associated surface protein Opa

The lacto-N-neotetraose-containing lipooligosaccharide (LOS) present on the surface of most Neisseria gonorrhoeae organisms may serve many important functions in gonococcal pathogenesis. This surface glycolipid contains the cross-reactive epitope to human paragloboside and can be sialylated by gonococci grown in the presence of CMP-N-acetylneuraminic acid. Another possible role for this glycolipid could be to mimic human asialocarbohydrates and act as a ligand for asialoglycoprotein receptors contained on numerous human cells. The most noted of this large family of receptors is that expressed on the surface of hepatic cells. In a model cell system, using the hepatoma tissue culture cell line HepG2, we wanted to investigate if the presence of this asialoglycoprotein receptor influenced the adherence and/or invasion of gonococci expressing the lacto-N-neotetraose structure. Piliated variants of the gonococcal wild-type strain 1291 and its isogeneic LOS mutant 1291E were used in adherence-invasion assays. This gonococcal strain is somewhat unusual in that it expresses large amounts of predominantly one species of LOS, thus reducing the complexity of interpreting the data. The data from these assays suggested that the Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)Glc carbohydrate structure on the wild-type LOS affected the adherence-invasion of gonococci into the HepG2 cells. In studies to determine whether the major hepatic asialoglycoprotein receptor was involved in these interactions, we found that the HepG2 cells contained two receptors which bound gonococcal LOS. One of these was the asialoglycoprotein receptor, and the data concerning this receptor will be reported elsewhere. The data on the second receptor are reported here. Purified, 125I-labeled gonococcal LOS was used to identify specific high-affinity LOS-binding sites. These binding experiments revealed one major binding site corresponding to a protein with a molecular mass of 70 kDa (p70). Several lines of evidence in this study suggested that the oligosaccharide region of LOS played an important role in LOS binding to the p70 of HepG2 cells. In addition, we show that this human LOS receptor has some similarities to the gonococcal Opa proteins.
COMPOSITION OF CORONAL MASS EJECTIONS

We analyze the physical origin of plasmas that are ejected from the solar corona. To address this issue, we perform a comprehensive analysis of the elemental composition of interplanetary coronal mass ejections (ICMEs) using recently released elemental composition data for Fe, Mg, Si, S, C, N, Ne, and He as compared to O and H. We find that ICMEs exhibit a systematic abundance increase of elements with first ionization potential (FIP) < 10 eV, as well as a significant increase of Ne as compared to quasi-stationary solar wind. ICME plasmas have a stronger FIP effect than slow wind, which indicates either that an FIP process is active during the ICME ejection or that a different type of solar plasma is injected into ICMEs. The observed FIP fractionation is largest during times when the Fe ionic charge states are elevated above QFe > 12.0. For ICMEs with elevated charge states, the FIP effect is enhanced by 70% over that of the slow wind. We argue that the compositionally hot parts of ICMEs are active region loops that do not normally have access to the heliosphere through the processes that give rise to solar wind. We also discuss the implications of this result for solar energetic particles accelerated during solar eruptions and for the origin of the slow wind itself.
Psychometric Evaluation of the Chinese Version of the Minnesota Living With Heart Failure Questionnaire

Background: Health-related quality of life has emerged as an important outcome in treating and managing patients with heart failure (HF). However, there is no existing instrument for use in Chinese populations. Objectives: To translate the Minnesota Living With Heart Failure Questionnaire (MLHFQ) into a Chinese version and to validate it in the clinical setting. Methods: This was a cross-sectional study. A convenience sample of 247 participants with New York Heart Association Class II to III chronic HF were recruited from a cardiovascular outpatient department in Taiwan. Results: The content validity index was 0.98. Construct validity was supported by exploratory factor analysis, which showed three factors with 20 of 21 items loading above . 50, explaining 71% of the variance. Total scale and subscale scores were correlated highly with the reference instruments, indicating satisfactory concurrent and convergent validity. The mean scores of the Chinese version of the MLHFQ were significantly higher among participants with HF than those from a non-HF comparison group. The instrument demonstrated high internal consistency (Cronbach's α of .95 for the scale and .93 to .95 for the subscales). Conclusion: The psychometric properties indicate that the Chinese version of the MLHFQ is a valid and reliable instrument for future research. This instrument provides a holistic approach for measuring health-related quality of life in New York Heart Association Class II and III HF patients.
Optimising the Management of a Heterogeneous Stand

The study presents a method for taking the heterogeneity of the stand into account in the optimisation of stand management. Heterogeneity refers to within-stand variation in stand density and/or other characteristics. A set of plots, corresponding to different sub-areas of the stand, represents the stand in calculations. Cuttings and other treatments of the plots are done simultaneously. The method was used to analyse how the optimal management depends on the heterogeneity of a Norway spruce (Picea abies (L.) Karst. ) and Scots pine (Pinus sylvestris L.) stand. The results supported the hypothesis that the heterogeneity of a stand decreases its optimal prior-thinning density. Also the remaining stand basal areas were lower in heterogeneous stands, especially in spruce. The effect of stand heterogeneity prior to the first commercial thinning still affected the timing of the second thinning, which had to be conducted earlier and at lower prior-thinning basal areas in heterogeneous stands. This happened despite the fact that the first thinning greatly decreased the within-stand variation in stand basal area. In addition, heterogeneity decreased the soil expectation value, net income and timber harvests.