Miracle-dz/UniFAR-FTUs huggingface dataset:
Three new serum markers for prostate cancer detection within a percent free PSA‐based artificial neural network

We aimed to evaluate the value of macrophage inhibitory cytokine 1 (MIC‐1), human kallikrein 11 (hK11) migration inhibitor factor (MIF) in comparison to prostate‐specific antigen (PSA) and %fPSA and also to develop a %fPSA‐based ANN with the new input factors to determine whether these additional markers can further eliminate unnecessary prostate biopsies.
Magnetization Precession by Hot Spin Injection

As electrons are injected at various energies into ferromagnetic material with their spin polarization vector perpendicular to the axis of the magnetization, we observe precessional motion of the spin polarization on the femtosecond time scale. Because of angular momentum conservation, the magnetization vector must precess as well. We show that spin injection will generate the precessional magnetization reversal in nanosized ferromagnetic bits. At reasonable injected current densities this occurs on the picosecond time scale.
CROSS‐VALIDATION OF VENTILATORY THRESHOLD PREDICTION EQUATIONS ON AEROBICALLY TRAINED MEN AND WOMEN

The purpose of this investigation was to determine the validity of the non–exercise-based equations of Davis et al. (13), Jones et al. (20), and Neder et al. (30) for estimating the ventilatory threshold (VT) in samples of aerobically trained men and women. One hundred and forty-four aerobically trained men (mean ± SD age, 41.0 ± 11.6 years; N = 83) and women (37.1 ± 9.0 years, N = 61) performed a maximal incremental test to determine &OV0312;O2max and observed VT on a cycle ergometer. The observed VT was determined by gas exchange measurements using the V-slope method (&OV0312;CO2/&OV0312;O2) in conjunction with analyses of the ventilatory equivalents (i.e., minute ventilation &OV0312;E/&OV0312;O2 and &OV0312;E/&OV0312;CO2) and end-tidal gas tensions (i.e., PETO2 and PETCO2) for oxygen and carbon dioxide. The predicted VT values from 14 equations were compared to the observed VT values by examining the constant error (CE), standard error of estimate (SEE), Pearson correlation coefficient (r), and total error (TE). The results of this investigation indicated that all 14 equations resulted in significant (p < 0.008) CE values ranging from 1.13 to 1.72 L·min−1 for the men and from 0.58 to 1.12 L·min−1 for the women. Furthermore, the SEE, r, and TE values ranged from 0.37 to 0.54, from 0.36 to 0.53, and from 0.68 to 1.81 L·min−1, respectively. The lowest TE values for the men and women represented 45 and 36% of the mean of the observed VT values, respectively. The results of this study indicated that the errors associated with all 14 equations were too large to be of practical value for estimating VT in aerobically trained men and women.
Responses of porcine epicardial venules to neurohumoral substances.

OBJECTIVE The coronary microvenous system may be determinant of ventricular distensibility and of resistance to coronary flow under conditions of arteriolar dilatation. Because responses of venules have not been well defined, in vitro responses of porcine venules and arterioles to neurohumoral substances were examined. METHODS Porcine subepicardial venules (125-250 microns in diameter) and arterioles (100-200 microns in diameter) were studied in a pressurised no flow state with video microscopical imaging and electronic dimension analysis. After precontraction of vessels with the thromboxane A2 analogue U46619, agents were applied extraluminally. RESULTS Responses of precontracted venules to sodium nitroprusside, adenosine, and adenosine 5' diphosphate (ADP) were comparable with those of similarly sized arterioles. By contrast, precontracted venules responded minimally to noradrenaline, whereas arterioles were dilated potently by this agent. Precontracted arterioles were dilated minimally by serotonin and were contracted slightly by acetylcholine, whereas venules dilated in response to either serotonin or acetylcholine. Serotonin and acetylcholine both produced greater contraction in non-precontracted arterioles than in non-precontracted venules. Responses of venules to most substances were minimally affected by changes in oxygen tension except when this was very low. Relaxation of venules by ADP, serotonin, and acetylcholine were all inhibited to varying degrees by indomethacin, methylene blue, and NG-methyl-L-arginine (L-NMMA). Both methylene blue and L-NMMA produced minimal but significant contraction of non-precontracted venules, indicating a small amount of basal release of endothelium derived relaxing factor. CONCLUSION Responses of coronary venules to acetylcholine, serotonin, and noradrenaline are opposite or of a significantly different magnitude when compared with the respective arteriolar responses. Relaxation of venules to ADP, serotonin, and acetylcholine is likely mediated at least in part by the release of endothelium derived relaxing factor and prostaglandins.
Brain phenotypes in two FGFR2 mouse models for Apert syndrome

Apert syndrome (AS) is one of at least nine disorders considered members of the fibroblast growth factor receptor (FGFR) ‐1, ‐2, and ‐3–related craniosynostosis syndromes. Nearly 100% of individuals diagnosed with AS carry one of two neighboring mutations on Fgfr2. The cranial phenotype associated with these two mutations includes coronal suture synostosis, either unilateral (unicoronal synostosis) or bilateral (bicoronal synostosis). Brain dysmorphology associated with AS is thought to be secondary to cranial vault or base alterations, but the variation in brain phenotypes within Apert syndrome is unexplained. Here, we present novel three‐dimensional data on brain phenotypes of inbred mice at postnatal day 0 each carrying one of the two Fgfr2 mutations associated with AS. Our data suggest that the brain is primarily affected, rather than secondarily responding to skull dysmorphogenesis. Our hypothesis is that the skull and brain are both primarily affected in craniosynostosis and that shared phenogenetic developmental processes affect both tissues in craniosynostosis of Apert syndrome. Developmental Dynamics 239:987–997, 2010. © 2010 Wiley‐Liss, Inc.
Intraspecific DNA variation in nuclear genes of the mosquito Aedes aegypti

Single nucleotide polymorphisms (SNPs) are an abundant source of genetic variation among individual organisms. To assess the usefulness of SNPs for genome analysis in the yellow fever mosquito, Aedes aegypti, we sequenced 25 nuclear genes in each of three strains and analysed nucleotide diversity. The average frequency of nucleotide variation was 12 SNPs per kilobase, indicating that nucleotide variation in Ae. aegypti is similar to that in other organisms, including Drosophila and the malaria vector Anopheles gambiae. Transition polymorphisms outnumbered transversion polymorphisms, at a ratio of about 2 : 1. We examined codon usage and confirmed that mutational bias favours G and C ending codons. Codon bias was most pronounced in highly expressed genes. Nucleotide diversity estimates indicated that substitution rates are positively correlated in coding and non‐coding regions. Nucleotide diversity varied from one gene to another. The unequal distribution of SNPs among Ae. aegypti nuclear genes suggests that single base variations are non‐neutral and are subject to selective constraints. Our analysis showed that ubiquitously expressed genes have lower polymorphism rates and are likely under strong purifying selection, whereas tissue specific genes and genes with a putative role in parasite defence exhibit higher levels of polymorphism that may be associated with diversifying selection.
Thermometer Code Based Modular Arithmetic

This paper presents a novel approach to perform modular arithmetic addition and subtraction using base-1 thermometer code data format for operands corresponding to the residues of the same modulus. Two n-bit thermometer code operands are first concatenated and logically shifted to produce a normalized 2n-bit thermometer code intermediate sum. Modulo operation is then applied to this 2n-bit intermediate sum to produce an n-bit datum corresponding to the modular sum of the two input operands. This approach greatly simplifies the modulo addition operation by eliminating the carry bit propagation during the arithmetic operation encountered when using the conventional base-2 binary code data format. It also enables practical applications of modular arithmetic for signal processing algorithms in a very efficient way. Circuit for implementing the modular arithmetic units using multiplexers and basic logic gates are also described in this paper.
Male gonadal axis function in patients with type 2 diabetes

Abstract Patients with type 2 diabetes have lower serum testosterone levels and a higher prevalence of hypogonadism than non-diabetic patients, independently of the metabolic control of disease. The mechanisms underlying a decrease in testosterone might be related to age, obesity and insulin resistance, often present in patients with type 2 diabetes. The increase in estrogens due to higher aromatase enzyme activity in increased adipose tissue might exert negative-feedback inhibition centrally. Insulin stimulates gonadal axis activity at all three levels and therefore insulin resistance might account for the lower testosterone production. Leptin exerts a central stimulatory effect but inhibits testicular testosterone secretion. Thus, resistance to leptin in obese subjects with type 2 diabetes determines lower central effects of leptin with lower gonadotropin-releasing hormone (GnRH) secretion and, on the other hand, hyperleptinemia secondary to leptin resistance inhibits testosterone secretion at the testicular level. However, lower testosterone levels in patients with diabetes are observed independently of age, weight and body mass index, which leads to the assumption that hyperglycemia per se might play a role in the decrease in testosterone. Several studies have shown that an overload of glucose results in decreased serum testosterone levels. The aim of this review is to assess changes in the male gonadal axis that occur in patients with type 2 diabetes.
End-Stage Renal Disease and Critical Limb Ischemia: A Deadly Combination?

Objectives: This study was planned to evaluate the prognostic impact of end-stage renal disease (ESRD) in patients with critical leg ischemia (CLI) undergoing infrainguinal revascularization. Materials and Methods: 1425 patients who underwent infrainguinal revascularization for CLI were the subjects of the present analysis. Ninety-five patients had ESRD (eGFR < 15 ml/min/m2), and of them 66 (70%) underwent percutaneous transluminal angioplasty and 29 (30%) underwent bypass surgery. Results: ESRD patients had significantly lower overall survival (at 3-year, 27.1% vs. 59.7%, p < 0.0001), leg salvage (at 3-year, 57.7% vs. 83.0%, p<0.0001), and amputation free survival (at 3-year, 16.2% vs. 52.9%, p < 0.0001) than patients with no or less severe renal failure. The difference in survival was even greater between 86 one-to-one propensity matched pairs (at 3-year, 23.1% vs. 67.3%, p < 0.0001). ESRD was an independent predictor of all-cause mortality (RR 2.46, 95%CI 1.85–3.26). Logistic regression showed that age ≥ 75 years was the only independent predictor of 1-year all-cause mortality (OR 4.92, 95%CI 1.32–18.36). Classification and regression tree analysis showed that age ≥ 75 years and, among younger patients, bypass surgery for leg ulcer and gangrene were associated with significantly higher 1-year mortality. Conclusions: Lower limb revascularization in patients with CLI and end-stage renal failure is associated with favourable leg salvage. However, these patients have a very poor survival and this may jeopardize any attempt of revascularization. Further studies are needed to identify ESRD patients with acceptable life expectancy and who may benefit from lower limb revascularization.
[The relationship between keratoconus and axial myopia].

PURPOSE To study the relationship between keratoconus and axial myopia. METHODS We prospectively studied refraction and ocular biometry in 100 consecutive keratoconus eyes, and 100 emmetropic eyes. Biometric data (including posterior segment length) were obtained by A-scan ultrasound, Orbscan, and ultrasonic pachymetry. Biometric measurements were compared in both groups. RESULTS Anterior chamber depth, posterior segment length and axial length were significantly different between the two groups (p<0.001). Axial length was significantly greater in the keratoconus group than in the emmetropic group (23.97 mm versus 23.21 mm, p<0.001). Posterior segment length was significantly greater in the keratoconus group than in the emmetropic group (16.54 mm versus 15.99 mm, p<0.001). In the keratoconus group, the posterior segment length and the subjective spherical equivalent were significantly correlated (rs=-0.45, p<0.001). CONCLUSION The increase in posterior segment length in the keratoconus group and the correlation between posterior segment length and spherical equivalent in the keratoconus group suggest a statistical relationship between keratoconus and axial myopia.
Use of potentially inappropriate medicines in elderly: A prospective study in medicine out-patient department of a tertiary care teaching hospital

Objective: The present study was undertaken with the aim to detect extent of drug use in elderly at medicine outpatient department at tertiary care hospital and to evaluate inappropriate prescribing with the help of Beers' criteria 2002. Materials and Methods: The study was carried out at medicine out patient department of our hospital. 407 geriatric patients were included during the study period of three and half months. The data was collected in a proforma which included the patients' details and the prescriptions. Results: The results reveal that 7.42% of total drugs were prescribed in an inappropriate manner and 23.59% of total patients received at least one inappropriate drug prescription. Administration of a drug which is avoided in elderly forms a common category of inappropriate drug use. Antihistamines, anticholinergic, sedatives and hypnotics and cardiac glycosides are the most common drug groups prescribed in inappropriate manner. Conclusion: To conclude, this study shows high prevalence of inappropriate use of drugs in geriatric practice suggesting urgent need for sincere efforts to improve the situation.
Impact of Molar Furcations on Photodynamic Therapy Outcomes: A 6-Month Split-Mouth Randomized Clinical Trial

The effectiveness of adjunctive photodynamic treatment (PDT) to non-surgical periodontal therapy has been shown to depend on initial periodontal status. As molar furcation involvement impairs healing response to non-surgical periodontal therapy, the aim of this study was to evaluate the impact of furcation involvement on PDT outcomes. Thirty-six patients suffering from severe chronic periodontitis were included in a 6-month split-mouth randomized clinical trial. PDT applications used the toluidine blue O and a light-emitting diode (LED) with a red spectrum. Repeated PDT applications were performed in addition to non-surgical periodontal treatment at baseline and at 3-months. Pocket probing depth (PPD), plaque index, bleeding on probing, and clinical attachment level were recorded at baseline, and again at 3- and 6-months. Furcation sites of molars were compared to other sites of molars and non-molars. Multilevel analysis showed no PDT effect in molar furcation sites while an additional significant reduction (odds ratio = 0.67) of pockets with PPD > 5 mm in other sites at 3-months was measured. PPD reduction appeared delayed in molar furcation sites treated with PDT. There is no additional apparent benefit to use PDT in molar furcation sites for the reduction of pockets with PPD > 5 mm contrary to other sites.
NON-INVASIVE APPROACH TO PREDICT THE CHOLESTEROL LEVEL IN BLOOD USING BIOIMPEDANCE AND NEURAL NETWORK TECHNIQUES

This paper presents a new non-invasive approach to predict the status of high total cholesterol (TC) level in blood using bioimpedance and the artificial neural network (ANN) techniques. The input parameters for the ANN model are acquired from a non-invasive bioelectrical impedance analysis (BIA) measurement technique. The measurement data were obtained from 260 volunteered participants. A total of 190 subject's data were used for the ANN training purpose and the remaining 70 subject's data were used for model testing. Six parameters from the BIA parameters were found to be significant predictors for TC level in blood using logistic regression analysis. The six input predictors for the ANN modeling are age, body mass index (BMI), body capacitance, basal metabolic rate, extracellular mass and lean body mass. Four ANN techniques such as the gradient descent with momentum, the resilient, the scaled conjugate gradient and the Levenberg–Marquardt were used and compared for predicting the high TC level in the blood. The finding showed that the resilient method was the best model with prediction accuracy, sensitivity, specificity and area under the curve value obtained from the test data were 82.9%, 85.4%, 79.3% and 0.83%, respectively.
Central Responsiveness to a Ghrelin Mimetic (GHRP‐6) is Rapidly Altered by Acute Changes in Nutritional Status in Rats

The hypothalamus appears to be more responsive to ghrelin and growth hormone secretagogues (GHS) in fasting, as reflected by a two‐ to three‐fold increase in the number of cells detected that express Fos protein in the arcuate nucleus, in 48‐h fasted rats compared to fed controls. Moreover, this increased hypothalamic responsiveness to GHS in fasting is regulated by the central action of exogenous leptin and insulin, although it is unknown whether these hormones mediate the changes in hypothalamic responsiveness to GHS associated with the fasting/fed state. In the present study, we show that refeeding with normal rat chow for only 2 h at the end of a 48‐h fast reversed the potentiation of the Fos response to GHRP‐6 observed in fasted rats. Circulating leptin and insulin levels remained significantly lower in refed rats compared to ad lib‐fed rats, suggesting that the change in the hypothalamic sensitivity brought about by refeeding was independent of these hormones. By contrast, 2 h of chow refeeding at the end of a fast restored plasma glucose levels to those of the fed state. Refeeding with sugar alone for 2 h at the end of a 48‐h fast also reduced the potentiated Fos response in fasting, indicating that elevated blood glucose can influence the central responsiveness to ghrelin/GHS. By contrast, infusion of the ileal satiety factor, PYY3‐36 (known to increase postprandially) did not alter the central responsiveness to GHRP‐6, although it suppressed feeding and body weight as expected. This study highlights the importance of nutritional status in regulating the action of exogenous GHS (and presumably endogenous ghrelin) on the hypothalamic circuits controlling food intake.
Resistance to HPPD-inhibiting herbicides in a population of waterhemp (Amaranthus tuberculatus) from Illinois, United States.

BACKGROUND A population of waterhemp in a seed maize production field in central Illinois, United States, was not adequately controlled after post-emergence applications of herbicides that inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD). RESULTS Progeny from the field population survived following treatment with mesotrione, tembotrione or topramezone applied to the foliage either alone or in combination with atrazine in greenhouse experiments. Dose-response experiments indicated that the level of resistance to the HPPD inhibitor mesotrione is at least tenfold relative to sensitive biotypes. CONCLUSION These studies confirm that waterhemp has evolved resistance to HPPD-inhibiting herbicides.
Muricauda beolgyonensis sp. nov., isolated from a tidal flat.

The taxonomic position of a Gram-staining-negative, non-motile, rod-shaped bacterium, strain BB-My12(T), which was isolated from a sediment sample collected from a tidal flat in Korea, was investigated by using a polyphasic approach. Strain BB-My12(T) grew optimally at 37 °C, at pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. In phylogenetic analyses based on 16S rRNA gene sequences, strain BB-My12(T) fell within the cluster comprising species of the genus Muricauda and appeared most similar to the type strains of Muricauda aquimarina, Muricauda lutimaris and Muricauda ruestringensis (97.5-97.6 % sequence similarity). The DNA G+C content was 45.0 mol%. Strain BB-My12(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 1), iso-C(17 : 0) 3-OH and iso-C(15 : 0) as the major cellular fatty acids. The polar lipids of strain BB-My12(T) were phosphatidylethanolamine and four unidentified lipids. The DNA-DNA relatedness values between strain BB-My12(T) and the type strains of the three species of the genus Muricauda that appeared most closely related were in the range 5-7 %. The genetic distinctiveness and some phenotypic properties indicated that strain BB-My12(T) did not belong to any established species of the genus Muricauda. Strain BB-My12(T) is therefore considered to represent a novel species of the genus Muricauda, for which the name Muricauda beolgyonensis sp. nov. is proposed. The type strain is BB-My12(T) ( = KCTC 23501(T) = CCUG 60800(T)).
The Yersinia enterocolitica Invasin Protein Promotes Major Histocompatibility Complex Class I- and Class II-Restricted T-Cell Responses

ABSTRACT Yersinia enterocolitica invasin (Inv) protein confers internalization into and expression of proinflammatory cytokines by host cells. Both events require binding of Inv to β1 integrins, which initiates signaling cascades including activation of focal adhesion complexes, Rac1, mitogen-activated protein kinase, and NF-κB. Here we tested whether Inv might be suitable as a delivery molecule and adjuvant if used as a component of a vaccine. For this purpose, hybrid proteins composed of Inv and ovalbumin (OVA) were prepared, applied as a coating to microparticles, and used for vaccination. Fusion of OVA to Inv did not significantly disturb the ability of Inv to promote host cell binding, internalization, and interleukin-8 (IL-8) secretion when applied as a coating to microparticles. The microparticles were used for vaccination of mice adoptively transferred with OVA-specific T cells from OT-1 or DO11.10 mice. Administration of OVA-Inv-coated microparticles induced OVA-specific T-cell responses. OVA-specific CD4 T cells produced both gamma interferon (IFN-γ) and IL-4 as determined by enzyme-linked immunosorbent assay. Likewise, pronounced OVA-specific CD8 T-cell responses associated with IFN-γ production were observed. Together, these results suggest that Inv might be an attractive tool in vaccination as it confers both host cell uptake and adjuvant activity by engagement of β1 integrins of host cells, which leads to CD4 as well as CD8 T-cell responses.
Clinicopathologic factors affecting patient survival and tumor recurrence after orthotopic liver transplantation for hepatocellular carcinoma.

THE role and value of orthotopic liver transplantation (OLTx) in the treatment of primary hepatic malignancies have been debated because of high tumor recurrence rates.l-I Certain clinicopathologic entities have been shown to influence patient survival rates for hepatocellular carcinoma (HCC). 3.4 In the current study, we analyzed various histopathologic features in the livers of 106 liver transplant recipients with HCC for their influence on patient outcome.