Miracle-dz/UniFAR-FTUs huggingface dataset:
Decreased Stimulation of CD4+ T Cell Proliferation and IL-2 Production by Highly Enriched Populations of HIV-Infected Dendritic Cells

APC infection and dysfunction may contribute to the immunopathogenesis of HIV disease. In this study, we examined immunologic function of highly enriched populations of HIV-infected monocyte-derived dendritic cells (DC). Compared with uninfected DC, HIV-infected DC markedly down-regulated surface expression of CD4. HIV p24+ DC were then enriched by negative selection of CD4+HIV p24− DC and assessed for cytokine secretion and immunologic function. Although enriched populations of HIV-infected DC secreted increased IL-12p70 and decreased IL-10, these cells were poor stimulators of allogeneic CD4+ T cell proliferation and IL-2 production. Interestingly, HIV-infected DC secreted HIV gp120 and the addition of soluble (s) CD4 (a known ligand for HIV gp120) to DC-CD4+ T cell cocultures restored T cell proliferation in a dose-dependent manner. By contrast, addition of antiretroviral drugs did not affect CD4+ T cell proliferation. Furthermore, recombinant HIV gp120 inhibited proliferation in uninfected cocultures of allogeneic DC and CD4+ T cells, an effect that was also reversed by addition of sCD4. In summary, we show that HIV gp120 produced by DC infected by HIV in vitro impairs normal CD4+ T cell function and that sCD4 completely reverses HIV gp120-mediated immunosuppression. We hypothesize that HIV-infected DC may contribute to impaired CD4+ T cell-mediated immune responses in vivo and that agents that block this particular immunosuppression may be potential immune adjuvants in HIV-infected individuals.
A Prospective Study to Assess the Efficacy and Patient Tolerance of Three Bowel Preparations for Colonoscopy

The adherence to the required clear liquid diet and a high-volume bowel cleansing solution prior to colonoscopy often leads to poor patient compliance and inadequate exams. This study evaluated the efficacy and patient tolerance of three bowel preparation regimens prior to colonoscopy. One hundred fourteen patients were randomized to one of three preparations. Group 1 was assigned a clear liquid diet/magnesium citrate and bisacodyl prep; group 2 was assigned to receive a diet kit/magnesium citrate and bisacodyl prep; and group 3 received a diet kit/polyethylene glycol electrolyte prep. The adequacy of bowel preparation was graded by the endoscopists performing the procedures. The preparations were rated by patients for tolerance, compliance, and side effects. The three methods of preparation did not prove to be significantly different in regards to efficacy of colon cleansing or patient tolerability. There was also no difference in compliance between the three regimens. There were no reported side effects in any of the study groups. This study suggests that a low-residue diet kit, given in conjunction with polyethylene glycol electrolyte or magnesium citrate and bisacodyl, produces adequate colon cleansing and patient tolerability.
Supercontinuum generation and nonlinear pulse propagation in photonic crystal fiber: influence of the frequency-dependent effective mode area

Numerical simulations are used to study the effect of the frequency dependence of the effective mode area in photonic crystal fiber on supercontinuum generation. We quantify how the frequency dependence of the effective area influences the propagation characteristics through a modified optical shock term and identify the major consequence as a reduction in the extreme long-wavelength edge of the supercontinuum spectrum. Our results show that, for the parameter regimes used in many previous supercontinuum generation experiments using near-infrared femtosecond pump sources around 800 nm, this effect would be expected to be negligible. However, for pumps in the 1000–1500 nm range, the inclusion of this effect would be expected to be crucial for accurate comparison of simulations with experiment.
Towards a multi‐foci approach to workplace aggression: A meta‐analytic review of outcomes from different perpetrators

Summary Using meta-analysis, we compare three attitudinal outcomes (i.e., job satisfaction, affective commitment, and turnover intent), three behavioral outcomes (i.e., interpersonal deviance, organizational deviance, and work performance), and four health-related outcomes (i.e., general health, depression, emotional exhaustion, and physical well being) of workplace aggression from three different sources: Supervisors, co-workers, and outsiders. Results from 66 samples show that supervisor aggression has the strongest adverse effects across the attitudinal and behavioral outcomes. Co-worker aggression had stronger effects than outsider aggression on the attitudinal and behavioral outcomes, whereas there was no significant difference between supervisor, co-worker, and outsider aggression for the majority of the health-related outcomes. These results have implications for how workplace aggression is conceptualized and measured, and we propose new research questions that emphasize a multifoci approach. Copyright # 2009 John Wiley & Sons, Ltd.
Characterization of Tetragenococcus halophila Populations in Indonesian Soy Mash (Kecap) Fermentation

Chinese- and Japanese-type fermented soy sauces are made of different plant materials. The lactic acid bacterium Tetragenococcus halophila is present and grows in both types. On the basis of the difference in sugar composition and content in the plant materials, differences in the populations of T. halophila bacteria were expected. However, obvious differences were found only regarding the utilization of l-arabinose. In the Chinese type, almost all isolates utilized l-arabinose, while in the Japanese type only 40% of the isolates did. Also, the population in the Japanese type was more heterogeneous regarding substrate utilization. Random amplified polymorphic DNA analysis revealed that the heterogeneous population at the Japanese-type industrial manufacturer was derived from only three strains at maximum. Genetic relatedness among isolates from different soy sauce manufacturers was low, but protein fingerprinting indicated that the isolates still belonged to one species.
Bilateral cavernous haemangiomas of the orbit

Editor,—The great majority of orbital tumours are unilateral. However, some conditions, such as idiopathic orbital inflammation (“pseudotumour”), granulomatous inflammation, lymphoid tumours, or metastases sometimes can be bilateral. Orbital cavernous haemangioma is almost always unilateral.1 2 We report a patient with bilateral orbital cavernous haemangiomas that caused unilateral proptosis and visual distortion. ### CASE REPORT A 37 year old man developed distorted vision in the inferior field of his left eye and was found to have mild left proptosis. There was mild swelling of the left optic disc and a small inferonasal visual field defect. Orbital computed tomography (CT) revealed bilateral orbital masses and the patient was referred to the oncology service for another opinion and management. His visual acuity was 6/6 …
The challenges of science journalism: The perspectives of scientists, science communication advisors and journalists from New Zealand

The news media play an important role in informing the public about scientific and technological developments. Some argue that restructuring and downsizing result in journalists coming under increased pressure to produce copy, leading them to use more public relations material to meet their deadlines. This article explores science journalism in the highly commercialised media market of New Zealand. Using semi-structured interviews with scientists, science communication advisors and journalists, the study finds communication advisors and scientists believe most media outlets, excluding public service media, report science poorly. Furthermore, restructuring and staff cuts have placed the journalists interviewed under increasing pressure. While smaller newspapers appear to be printing press releases verbatim, metropolitan newspaper journalists still exercise control over their use of such material. The results suggest these journalists will continue to resist increasing their use of public relations material for some time to come.
Antibacterial effects of Solanum tuberosum peel ethanol extract in vitro

Introduction: Today, medicinal plants are being widely used due to being natural, available, and cheaper than synthetic drugs and having minimum side effects. Since there were reports about the antibacterial properties of Solanum tuberosum (SE), the aim of this study was to investigate the antibacterial effects of SE ethanol extract in vitro condition on Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae Methods: Ethanol extract of SE peel was prepared by maceration method. Initially, antibacterial activity of ethanol extract of SE was qualitatively determined by disk diffusion test; then, the minimum inhibitory concentration and minimum bactericidal concentration were qualitatively determined by micro-dilution method. Results: SE peel extract had antibacterial properties and its effect was more pronounced on gram- positive bacteria, especially S. aureus (0.62±0.00 mg/ml). The extract had antibacterial activity on gram-negative bacteria, P. aeruginosa, too (8.33±2.88 mg/ml). Conclusion: SE peel extract has antibacterial activity and its effect on gram-positive bacteria was more pronounced than the investigated gram-negative bacteria. Therefore, it is suggested that SE peel constituent compounds be determined and to determine the exact mechanism of its antibacterial properties, and more comprehensive research be done to apply it, clinically. A R T I C L E I N F O
Oral immunization of interleukin-4 (IL-4) knockout mice with a recombinant Salmonella strain or cholera toxin reveals that CD4+ Th2 cells producing IL-6 and IL-10 are associated with mucosal immunoglobulin A responses

Mucosal immunoglobulin A (IgA) responses are often associated with Th2-type cells and derived cytokines, and interleukin-4 (IL-4) knockout (IL-4-/-) mice with impaired Th2 cells respond poorly to oral antigens. However, we have noted that IL-4-/- mice have normal mucosal IgA levels, which led us to query whether different oral delivery systems could elicit mucosal immunity. Two oral regimens were used: (i) a live recombinant Salmonella strain which expresses fragment C (ToxC) of tetanus toxin, and (ii) soluble tetanus toxoid (TT) with cholera toxin (CT) as an adjuvant. Oral immunization of IL-4-/- mice with recombinant Salmonella vaccine expressing ToxC induced brisk mucosal IgA and serum IgG (mainly IgG2a) anti-TT antibody responses. TT-specific CD4+ T cells from spleen or Peyer's patches produced gamma interferon, indicative of Th1 responses; however, IL-6 and IL-10 were also seen. Oral immunization of IL-4-/- mice with TT and CT induced weak mucosal IgA to TT; however, brisk IgA anti-CT-B responses and CT-B-specific CD4+ T cells producing IL-6 and IL-10 were also noted. These results show that although IL-4-dependent antibody responses are impaired, mucosal IgA responses are induced in IL-4-/- mice. These result suggest that certain cytokines, i.e., IL-6 and IL-10 from Th2-type cells, play an important compensatory role in the induction and regulation of mucosal IgA responses.
Comparison of a silicon carbide coated stent versus a noncoated stent in humans: the Tenax- versus Nir-Stent Study (TENISS).

PURPOSE Stents coated with hypothrombogenic silicon carbide (a-SiC:H) exhibited low restenosis rates in the rabbit and in an observational study in humans. Thus, the clinical and angiographic outcome was assessed in a large multicenter study. MATERIAL AND METHODS Four hundred and ninety-seven patients (63.4 +/- 9.8 years) were randomized to either receive the a-SiC:H-coated Tenax stent or the stainless steel Nir stent. Lesions (diameter > or = 2.8 mm, length < 20 mm) were covered with one single stent. RESULTS Fifty-one of 497 (10.3%) patients were excluded for protocol violation. Three hundred and forty-two of 446 (76.7%) patients presented for scheduled angiographic follow-up after 4.7 +/- 1.2 months and 29 of 446 (6.5%) prematurely. In-hospital complications comprised two deaths (0.8%) (P > 0.99) and one (0.4%) (P > 0.99) CK-elevation in each group, target lesion revascularization in 5 of 250 (2%) of the Tenax and 4 of 244 (1.6%) of the Nir sample (P > 0.99), and subacute thrombosis in 2 of 250 (0.8%) of the Tenax patients (P = 0.5). In the Tenax/Nir patients mean percent diameter stenosis decreased from 82.3 +/- 9.1%/80.7 +/- 8.4% (P = 0.49) to 17.6 +/- 5.5%/17.6 +/- 5.5% (P = 0.99) postprocedure and increased to 34.5 +/- 21.5%/34.2 +/- 23.1% (P = 0.90) at follow-up. CONCLUSIONS Thus, there appears to be no advantage of the silicon carbide coated stent over a stainless steel stent after 4.7 +/- 1.2 months with regard to clinical and angiographic restenosis rates.
Reasoning about conditional constraint specification problems and feature models

Abstract Product configuration is a major industrial application domain for constraint satisfaction techniques. Conditional constraint satisfaction problems (CCSPs) and feature models (FMs) have been developed to represent configuration problems in a natural way. CCSPs are like constraint satisfaction problems (CSPs), but they also include potential variables, which might or might not exist in any given solution, as well as classical variables, which are required to take a value in every solution. CCSPs model, for example, options on a car, for which the style of sunroof (a variable) only makes sense if the car has a sunroof at all. FMs are directed acyclic graphs of features with constraints on edges. FMs model, for example, cell phone features, where utility functions are required, but the particular utility function “games” is optional, but requires Java support. We show that existing techniques from formal methods and answer set programming can be used to naturally model CCSPs and FMs. We demonstrate configurators in both approaches. An advantage of these approaches is that the model builder does not have to reformulate the CCSP or FM into a classic CSP, converting potential variables into classical variables by adding a “does not exist” value and modifying the problem constraints. Our configurators automatically reason about the model itself, enumerating all solutions and discovering several kinds of model flaws.
Nonsystemic vasculitic neuropathy

Background: Nonsystemic vasculitic neuropathy (NSVN) is an uncommon disorder. Few series with small numbers of patients have been reported. The prognosis and treatment of patients presenting with NSVN remain uninvestigated. The authors sought to address these issues by assembling a large retrospective cohort with extended follow-up. Methods: All nerve biopsies performed over 20 years were reviewed; cases with definite, probable, or possible vasculitis were segregated for clinical correlation. Patients satisfying clinical criteria for NSVN at presentation were selected. Clinicopathologic, treatment, and outcome measures were analyzed in patients followed for ≥6 months. Results: A total of 48 patients (30 women, 18 men) with a median of 63 months of follow-up were identified. Most patients (85%) had extensive, overlapping involvement of multiple nerves. Only one had a symmetric polyneuropathy. Most neuropathies (96%) were painful. In 96%, nerve damage was distally accentuated, but most had concurrent proximal weakness. Diagnostic sensitivity was 58% for superficial peroneal nerve/peroneus brevis muscle biopsy and 47% for sural nerve biopsy. Combination corticosteroid/cytotoxic therapy was more effective than corticosteroid monotherapy in inducing remission and improving disability, with trends toward reduced relapses and chronic pain. Treatment with cyclophosphamide for >6 months decreased the relapse rate, which was 46% for all patients. Disease/treatment-related mortality was 10%. Six percent developed cutaneous involvement. Although chronic pain persisted in 60% of survivors, 80% had good outcomes. Conclusions: NSVN nearly always presents as an asymmetric, distally accentuated, painful, sensorimotor polyneuropathy. Risks for systemic spread and death are small, and, aside from pain, neurologic prognosis is unexpectedly good. Although this was not a randomized controlled trial, combination therapy produced the best outcome in this cohort.
“Non-lethal” weapons: precipitating a new arms race

Armies are purported to have already deployed dazzling devices, calmatives, entangling agents, adhesives (“stickums”), material that makes any surface too slippery to walk on (“slickums”), devices generating infrasound or electromagnetic waves, and devices for riot control.1 2 Other possible devices are sprays to weaken vehicle or plane parts, electromagnetic beams to confuse computers, and bacteria to degrade fuel. The term applied to this new generation of military technology is “non-lethal” weapons; it implies that military operations can avoid death and serious injury. Should not the medical profession rejoice? Let us first examine the terminology. A “weapon” is something that is designed to cause bodily harm; technologies designed specifically to damage inanimate objects should not be considered in the same context.1 “Non-lethal” implies zero fatalities, but such an objective is acknowledged to be unrealistic, giving rise to alternative phrases such as “less than lethal” or “sub-lethal. ”1 These terms carry the further implication that conventional antipersonnel …
Relationship between chest port catheter tip position and port malfunction after interventional radiologic placement.

PURPOSE The relationship between catheter tip position of implanted subcutaneous chest ports and subsequent port malfunction was investigated. Tip movement from initial supine position to subsequent erect position was also evaluated. MATERIALS AND METHODS Patients who underwent imaging-guided internal jugular chest port placement between July 2001 and May 2003 were identified with use of a quality-assurance database. Sixty-two patients were included in the study (22 men and 40 women), with a mean age of 58 years (range, 27-81 years). Catheter tip location on the intraprocedural chest radiograph was determined with use of two methods. First, the distance from the right tracheobronchial angle (TBA) was recorded (TBA distance). Second, tip location was classified into six anatomic regions: 1, internal jugular veins; 2, brachiocephalic veins; 3, superior vena cava (SVC; n = 11); 4, SVC/right atrial junction (n = 22); 5, upper half of right atrium (n = 25); and 6, lower half of right atrium (n = 4). For the duration of follow-up, catheter tip location was documented, as were all episodes of catheter malfunction. RESULTS Patients with catheter tips initially placed in position 3 had a higher risk of port malfunction (four of 11; 36%) than patients with catheter tips located in position 5 (two of 25; 8%). This difference narrowly fell short of statistical significance (P =.057). When comparing intraprocedural chest radiographs to the first erect chest radiographs, significant upward tip movement was noted. The tips migrated cephalad an average of 20 mm (P =.003) and 1.0 position units (P =.001). DISCUSSION Catheter tips placed in the SVC tended to have a greater risk of port malfunction compared with those positioned in the right atrium. Chest ports migrated cephalad between the supine and erect positions.
Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites

Mammalian ageing features biological attrition evident at cellular, genetic and epigenetic levels. Mutation of mitochondrial DNA, and nuclear DNA methylation changes are well established correlates of ageing. The methylation of mitochondrial DNA (mtDNA) is a new and incompletely described phenomenon with unknown biological control and significance. Here we describe the bisulphite sequencing of mtDNA from 82 individuals aged 18‐91 years. We detected low and variable levels of mtDNA methylation at 54 of 133 CpG sites interrogated. Regression analysis of methylation levels at two CpG sites (M1215 and M1313) located within the 12S ribosomal RNA gene showed an inverse correlation with subject age suggesting their utility as epigenetic markers of ageing.
The West African Monsoon

This chapter provides a summary of the recent advances made on the understanding and simulation of the coupled atmosphere-ocean-land system of the West African Monsoon (WAM) and of its variability on a wide range of space and timescales. More specifically we highlight the interannual to climate scales, the intraseasonal variability, weather systems and their predictability, the surface atmosphere coupling and the coupling with dust.
Loss of Dendritic HCN1 Subunits Enhances Cortical Excitability and Epileptogenesis

Hyperpolarization-activated cation nonselective 1 (HCN1) plasticity in entorhinal cortical (EC) and hippocampal pyramidal cell dendrites is a salient feature of temporal lobe epilepsy. However, the significance remains undetermined. We demonstrate that adult HCN1 null mice are more susceptible to kainic acid-induced seizures. After termination of these with an anticonvulsant, the mice also developed spontaneous behavioral seizures at a significantly more rapid rate than their wild-type littermates. This greater seizure susceptibility was accompanied by increased spontaneous activity in HCN1−/− EC layer III neurons. Dendritic Ih in these neurons was ablated, too. Consequentially, HCN1−/− dendrites were more excitable, despite having significantly more hyperpolarized resting membrane potentials (RMPs). In addition, the integration of EPSPs was enhanced considerably such that, at normal RMP, a 50 Hz train of EPSPs produced action potentials in HCN1−/− neurons. As a result of this enhanced pyramidal cell excitability, spontaneous EPSC frequency onto HCN1−/− neurons was considerably greater than that onto wild types, causing an imbalance between normal excitatory and inhibitory synaptic activity. These results suggest that dendritic HCN channels are likely to play a critical role in regulating cortical pyramidal cell excitability. Furthermore, these findings suggest that the reduction in dendritic HCN1 subunit expression during epileptogenesis is likely to facilitate the disorder.