Miracle-dz/UniFAR-FTUs huggingface dataset:
Correlates of health in adolescent elite athletes and adolescents: A cross-sectional study of 1016 adolescents

Abstract The primary aim of this cross-sectional study was to compare subjective correlates of health, such as nutrition, self-esteem, sleep and stress, between adolescent elite athletes (n = 625) and a reference group of adolescents (n = 391) not involved in elite sports. A secondary aim was to study predictors for self-perceived stress. A questionnaire was e-mailed to all participants containing valid questions about competence-based self-esteem, nutrition, self-perceived stress and sleep. Our results show that adolescent elite athletes reported significantly (p < .001) lower self-perceived stress, competence-based self-esteem, a more varied diet and more time of sleep, compared to adolescents not involved in elite sports (Hedges’g 0.31–0.82). Female adolescent elite athletes and female adolescents, compared to their male peer group, reported poorer values for competence-based self-esteem (elite, Hedges’g 0.29; adolescents, Hedges’g 0.30) and self-perceived stress (elite, Hedges’g 0.63; adolescents, Hedges’g 0.60). The linear mixed effect model revealed several significant (p < .05) predictors (sex, general well-being, competence-based self-esteem, sleep during weekdays, BMI, training volume) associated with self-perceived stress in adolescent elite athletes. In conclusion, differences in correlates of health with a moderate to strong effect sizes were found between the two groups, where elite athletes surprisingly reported less stress, competence-based self-esteem, a more varied diet and more time of sleep. Even if injury risk is high in adolescent elite athletes and pressure from multiple stakeholders is evident, it appears that the athletes still have better conditions for a good subjective health, based on self-reported measures, compared to adolescents not involved in elite sports.
A New Multilevel CART Algorithm for Multilevel Data with Binary Outcomes

Abstract The multilevel logistic regression model (M-logit) is the standard model for modeling multilevel data with binary outcomes. However, many assumptions and restrictions should be considered when applying this model for unbiased estimation. To overcome these limitations, we proposed a multilevel CART (M-CART) algorithm which combines the M-logit and single level CART (S-CART) within the framework of the expectation-maximization. Simulation results showed that the proposed M-CART provided substantial improvements on classification accuracy, sensitivity, and specific over the M-logit, S-CART, and single level logistic regression model when modeling multilevel data with binary outcomes. This benefit of using M-CART was consistently found across different conditions of sample size, intra-class correlation, and when relationships between predictors and outcomes were nonlinear and nonadditive.
ANALISIS BELANJA PEMERINTAH DAN INVESTASI SWASTA TERHADAP PERTUMBUHAN EKONOMI DAN DAMPAKNYA TERHADAP KEMISKINAN DI PROVINSI SULAWESI UTARA

Pertumbuhan ekonomi masih merupakan tujuan utama dan indikator penting keberhasilan pembangunan ekonomi daerah, dengan pertumbuhan Ekonomi yang tinggi diharapkan berpenagruh positif bagi kesejahtraan masyarakat dengan berkurangnya kemiskinan. Sulawesi Utara adalah salah satu pusat perekonomian di pulau Sulawesi, dalam meningkatkan Pertumbuhan ekonomi di daerah ini membutuhkan komitmen kuat dari pihak pemerintah dan swasta. Penelitian ini bertujuan untuk menganalisis bagaimana pengaruh Belanja Pemerintah dan Investasi Swasta terhadap pertumbuhan ekonomi dan dampak terhadap kemiskinan di Provinsi Sulawesi Utara. Teknik analisis yang di gunakan adalah model analisis path dengan data sekunder. Hasil penelitian menunjukan bahwa investasi swasta memiliki pengaruh yang tidak signifikan terhadap pertumbuhan ekonomi sedangkan belanja pemerintah memiliki pengaruh signifikan terhadap pertumbuhan ekonomi. Secara simultan invesatsi swasta dan belanja pemerintah memiliki pengaruh yang tidak signifikan terhadap pertumbuhan ekonomi, besarnya pengaruh investasi swasta dan belanja pemerintah melalui pertumbuhan ekonomi tidak dapat di jadikan variabel intervening.
Covalent labeling and mass spectrometry reveal subtle higher order structural changes for antibody therapeutics

ABSTRACT Monoclonal antibodies are among the fastest growing therapeutics in the pharmaceutical industry. Detecting higher-order structure changes of antibodies upon storage or mishandling, however, is a challenging problem. In this study, we describe the use of diethylpyrocarbonate (DEPC)-based covalent labeling (CL) – mass spectrometry (MS) to detect conformational changes caused by heat stress, using rituximab as a model system. The structural resolution obtained from DEPC CL-MS is high enough to probe subtle conformation changes that are not detectable by common biophysical techniques. Results demonstrate that DEPC CL-MS can detect and identify sites of conformational changes at the temperatures below the antibody melting temperature (e.g., 55 ᴼC). The observed labeling changes at lower temperatures are validated by activity assays that indicate changes in the Fab region. At higher temperatures (e.g., 65 ᴼC), conformational changes and aggregation sites are identified from changes in CL levels, and these results are confirmed by complementary biophysical and activity measurements. Given the sensitivity and simplicity of DEPC CL-MS, this method should be amenable to the structural investigations of other antibody therapeutics.
How does the process of group singing impact on people affected by cancer? A grounded theory study

Objective This study aimed to build an understanding of how the process of singing impacts on those who are affected by cancer, including patients, staff, carers and those who have been bereaved. Design A qualitative study, informed by a grounded theory approach. Setting and participants Patients with cancer, staff, carers and bereaved who had participated for a minimum of 6 weeks in one of two choirs for people affected by cancer. Methods 31 participants took part in Focus Group Interviews lasting between 45 min and an hour, and 1 participant had a face-to-face interview. Findings Four overarching themes emerged from the iterative analysis procedure. The overarching themes were: building resilience, social support, psychological dimensions and process issues. Following further analyses, a theoretical model was created to depict how building resilience underpins the findings. Conclusion Group singing may be a suitable intervention for building resilience in those affected by cancer via an interaction between the experience and impact of the choir.
Impact of Slice Thickness on the Predictive Value of Lung Cancer Screening Computed Tomography in the Evaluation of Coronary Artery Calcification

Background Image reconstruction thickness may impact quantitative coronary artery calcium scoring (CACS) from lung cancer screening computed tomography (LCSCT), limiting its application in practice. Methods and Results We evaluated Agatston‐based quantitative CACS from 1.25‐mm LCSCT and cardiac computed tomography for agreement in 87 patients. We then evaluated Agatston‐based quantitative CACS from 1.25‐, 2.5‐, and 5.0‐mm slice thickness LCSCT for agreement in 258 patients. Secondary analysis included the impact of slice thickness on predictive value of 4‐year outcomes. Median age of patients who underwent 1.25‐mm LCSCT and cardiac computed tomography was 63 years (interquartile interval, 57, 68). CACS from 1.25‐mm LCSCT and cardiac computed tomography demonstrated a strong Pearson correlation, R=0.9770 (0.965, 0.985), with good agreement. The receiver operating characteristic curve areas under the curve for cardiac computed tomography and LCSCT were comparable at 0.8364 (0.6628, 1.01) and 0.8208 (0.6431, 0.9985), respectively (P=0.733). Median age of patients who underwent LCSCT with 3 slice thicknesses was 66 years (interquartile interval, 63, 73). Compared with CACS from 1.25‐mm scans, CACS from 2.5‐ and 5.0‐mm scans demonstrated strong Pearson correlations, R=0.9949 (0.9935, 0.996) and R=0.9478 (0.9338, 0.959), respectively, though bias was largely negative for 5.0‐mm scans. Receiver operating characteristic curve areas under the curve for 1.25‐, 2.5‐, and 5.0‐mm scans were comparable at 0.7040 (0.6307, 0.7772), 0.7063 (0.6327, 0.7799), and 0.7194 (0.6407, 0.7887), respectively (P=0.6487). When using individualized high‐risk thresholds derived from respective receiver operating characteristic curves, all slice thicknesses demonstrated similar prognostic value. Conclusions Slice thickness is an important consideration when interpreting Agatston CACS from LCSCTs. Despite the absence of ECG gating, it appears reasonable to report CACS from either 1.25‐ or 2.5‐mm slice thickness LCSCT to help stratify cardiovascular risk. Conversely, 5.0‐mm scans largely underidentify calcium, limiting practical use within the established CACS values used to categorize cardiovascular risk.
Counseling Programs: A Guide to Evaluation

Introduction Vignette 1 Determining Students' Preferences for Small Groups Vignette 2 Evaluating Individual Counseling Services Vignette 3 Using Control Groups in Program Evaluation Vignette 4 Evaluating Classroom Guidance Vignette 5 Assessing Parents' Opinions of the Overall Program Vignette 6 Surveying Teachers' Opinions of Selected Progam Components
Oncogenic value of microRNA-15b-5p in hepatocellular carcinoma and a bioinformatics investigation

miR-15b-5p has frequently been reported to function as a biomarker in some malignancies; however, the function of miR-15b-5p in hepatocellular carcinoma (HCC) and its molecular mechanism are still not well understood. The present study was designed to confirm the clinical value of miR-15b-5p and further explore its underlying molecular mechanism. A comprehensive investigation of the clinical value of miR-15b-5p in HCC was investigated by data mining The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets as well as literature. In addition, intersected target genes of miR-15b-5p were predicted using the miRWalk database and differentially expressed genes of HCC from TCGA. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were carried out. Then, a protein-protein interaction network (PPI) was constructed to reveal the interactions between some hub target genes of miR-15b-5p. The miR-15b-5p expression level in HCC was predominantly overexpressed compared with non-HCC tissues samples (SMD=0.618, 95% CI: 0.207, 1.029; P<0.0001) based on 991 HCC and 456 adjacent non-HCC tissue samples. The pooled summary receiver operator characteristic (SROC) of miR-15b-5p was 0.81 (Q*=0.74), and the pooled sensitivity and specificity of miR-15b-5p in HCC were 72% (95% CI: 69–75%) and 68% (95% CI: 65–72%), respectively. Bioinformatically, 225 overlapping genes were selected as prospective target genes of miR-15b-5p in HCC, and profoundly enriched GO terms and KEGG pathway investigation in silico demonstrated that the target genes were associated with prostate cancer, proximal tubule bicarbonate reclamation, heart trabecula formation, extracellular space, and interleukin-1 receptor activity. Five genes (ACACB, RIPK4, MAP2K1, TLR4 and IGF1) were defined as hub genes from the PPI network. The high expression of miR-15b-5p could play an essential part in hepatocarcinogenesis through diverse regulation approaches.
MRI of herniated nucleus pulposus : correlation with clinical findings, determinants of spontaneous resorption and effects of anti-inflammatory treatments on spontaneous resorption

The purpose of the current study was to evaluate the intercorrelations of magnetic resonance imaging (MRI) findings and clinical symptoms and signs in sciatic patients. Furthermore, determinants of spontaneous HNP resorption and the effect of anti-inflammatory treatments (periradicular methylprednisolone injection and intravenous infliximab) on spontaneous HNP resorption were evaluated. MRI follow-up was performed at baseline, after two months, after six months and after one-year for patients with unilateral sciatica to evaluate determinants of spontaneous HNP resorption and the effect of periradicular methylprednisolone injection on spontaneous HNP resorption. At baseline the study population consisted of 160 patients (group A). MRI follow-up for 21 patients with unilateral sciatica was performed at baseline and after two weeks, after three months and after six months to evaluate the effect of infliximab, a monoclonal TNFα antagonist, infusion on spontaneous HNP resorption (group B). Patients in group A were randomized to receive either periradicular saline or methylprednisolone. Volume of HNP, extent and thickness of enhancement (in Gd-DTPA MRI) and degree of disc displacement were measured and the symptoms and signs were followed repeatedly. The extent of rim enhancement correlated significantly with the degree of disc displacement. The duration of sciatic symptoms correlated negatively with enhancement parameters. The clinical symptoms did not correlate significantly with the different enhancement parameters or disc herniation volume. Achilles reflex abnormality correlated significantly with all enhancement parameters for lesions at L5-S1. Significant decrease in HNP volume occurred from baseline to two moths, and even more so during the whole one year follow-up period. Higher baseline scores of rim enhancement thickness, higher degree of HNP displacement in the Komori classification and age category of 41-50 years were associated with a higher resorption rate. Clinical symptoms alleviation occurred concordantly with a faster resorption rate. No significant difference was noted in the decrease of HNP volume in the saline and methylprednisolone injection groups in follow-up imaging during one year. The enhancement parameters (thickness and extent of rim enhancement) did not differ significantly in the different treatment groups. In group B, 11 patients received intravenous infliximab and 10 saline. Baseline demographic data, pain scores, and clinical status, did not differ between the treatment groups. HNP volume decreased significantly in both groups (P<0.01). There was no significant difference in HNP volume changes between the treatment groups. By two weeks, enhancement thickness increased significantly in the infliximab compared placebo group (p=0.003). Two patients in each group required back surgery prior to the 6-month assessment.
Function of a strand-separation pin element in the PriA DNA replication restart helicase

DNA helicases are motor proteins that couple the chemical energy of nucleoside triphosphate hydrolysis to the mechanical functions required for DNA unwinding. Studies of several helicases have identified strand-separating “pin” structures that are positioned to intercept incoming dsDNA and promote strand separation during helicase translocation. However, pin structures vary among helicases and it remains unclear whether they confer a conserved unwinding mechanism. Here, we tested the biochemical and cellular roles of a putative pin element within the Escherichia coli PriA DNA helicase. PriA orchestrates replication restart in bacteria by unwinding the lagging-strand arm of abandoned DNA replication forks and reloading the replicative helicase with the help of protein partners that combine with PriA to form what is referred to as a primosome complex. Using in vitro protein–DNA cross-linking, we localized the putative pin (a β-hairpin within a zinc-binding domain in PriA) near the ssDNA–dsDNA junction of the lagging strand in a PriA–DNA replication fork complex. Removal of residues at the tip of the β-hairpin eliminated PriA DNA unwinding, interaction with the primosome protein PriB, and cellular function. We isolated a spontaneous intragenic suppressor mutant of the priA β-hairpin deletion mutant in which 22 codons around the deletion site were duplicated. This suppressor variant and an Ala-substituted β-hairpin PriA variant displayed wildtype levels of DNA unwinding and PriB binding in vitro. These results suggest essential but sequence nonspecific roles for the PriA pin element and coupling of PriA DNA unwinding to its interaction with PriB.
[Ten years of alcohol intoxication among young people in The Netherlands].

OBJECTIVE To provide insight into the number of adolescents admitted to hospital with alcohol intoxication in the period 2007-2016. DESIGN Observational cross-sectional study. METHODS We used data from the hospital admissions for alcohol intoxication among minors that had been reported to the Dutch Paediatric Surveillance System (NSCK). We also used data on minors who had been admitted to hospital for other alcohol-related reasons. RESULTS In the period 2007-2016, 4,674 minors were admitted to a hospital in the Netherlands with alcohol intoxication. The number of minors admitted with alcohol intoxication increased annually from 2007 to 2011, after which it became reasonably stable, with a peak in 2015. The average age at which a minor was admitted increased (2007: 14.9 years; 2016: 15.5 years), and this also applied to the duration of loss of consciousness (2007: 2.24 hours; 2016: 3.12 hours). Parents of young people admitted with alcohol intoxication have become markedly stricter; 69% had given permission for consumption of alcohol in 2011; in 2016 that had decreased to 19%. CONCLUSION Raising the minimum age for obtaining low-alcohol drinks, the creation of better awareness of the consequences of alcohol use and the opening of the Outpatient Adolescents and Alcohol Clinics seem to have had an effect. The average age of young people with alcohol intoxication has increased and parents have become stricter, but binge-drinking is still a serious problem. Further research is needed into characteristics and treatment of the group of young people who end up in hospital.
Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats

Activated macrophages play a vital role in rheumatoid arthritis (RA) pathophysiology. CD44 is an overexpressed receptor on activated macrophages that is a potential target site for RA treatment. In this study, we prepared hyaluronic acid (HA) coated acid-sensitive polymeric nanoparticles (HAPNPs) composed of egg phosphatidylcholine, polyethylenimine, and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) loaded with dexamethasone (Dex) for the treatment of RA. PCADK was used to form polymeric cores because of its acid-sensitivity. The HAPNPs were about 150 nm in size and had a zeta potential of −2.84 mV. The release rate of Dex from HAPNPs/Dex in vitro increased markedly when the pH decreased from 7.4 to 4.5, indicating that the HAPNPs were pH-sensitive. In a cellular uptake study, stronger fluorescence signals were observed in activated macrophages treated with HAPNPs, suggesting that HAPNPs could be effective nanodevices target to activated macrophages. In rats with adjuvant-induced arthritis, HAPNPs could inhibited the progression of RA. Taken together, these results suggest that the HAPNPs could be useful in RA therapy.
Intra and Interpersonal Factors in the Use of Personal Therapy by Trainee Clinical Psychologists

5 Introduction: 6 Outline 6 The Philosophical Position of Clinical Psychology 6 Personal and Professional Development (PPD) 10 PPD: What it is and why it matters 12 Fostering Personal and Professional Development by courses 14 Improving reflective practice in trainees 17 Qualification and the use of personal therapy 19 How often is personal therapy actually used 21 Research on personal therapy since 1998 23 Personal therapy in training as help-seeking 28 The non-use of personal therapy 30 The impact of others 31 The decision-making process: to use or not use personal therapy 33 Limitations in the research 37 Aim of the study 39 Hypothesis 40 Method 41 Design 41 Participants Target Population Response rate and sample 41 41 42 Measures 44 Procedure 52
Multichannel Time–Frequency Complexity Measures for the Analysis of Age-Related Changes in Neuromagnetic Resting-State Activity

We propose new multichannel time–frequency complexity measures to evaluate differences on magnetoencephalograpy (MEG) recordings between healthy young and old subjects at rest at different spatial scales. After reviewing the Rényi and singular value decomposition entropies based on time–frequency representations, we introduce multichannel generalizations, using multilinear singular value decomposition for one of them. We test these quantities on synthetic data, illustrating how the introduced complexity measures focus on number of components, nonstationarity, and similarity across channels. Friedman tests are used to confirm the differences between young and old groups, and heterogeneity within groups. Experimental results show a consistent increase in complexity measures for the old group. When analyzing the topographical distribution of complexity values, we found clusters in the frontal sensors. The complexity measures here introduced seem to be a better indicator of the neurophysiologic changes of aging than power envelope connectivity. Here, we applied new multichannel time–frequency complexity measures to resting-state MEG recordings from healthy young and old subjects. We showed that these features are able to reveal regional clusters. The multichannel time–frequency complexities can be used to monitor the aging of subjects. They also allow a mutual information approach, and could be applied to a wider range of problems.
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement

Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae.