Miracle-dz/UniFAR-FTUs huggingface dataset:
The increase in emotional symptoms of novice clinical psychology trainees compared with a control cohort

espanolResumen La investigacion transversal ha mostrado que los practicantes de psicologia clinica suelen enfrentar un amplio rango de estresores relacionados con la practica clinica y tienden a presentar mayor grado de malestar emocional que otros estudiantes de psicologia. Sin embargo, ningun estudio longitudinal ha analizado este hecho. Este articulo analiza la evolucion de los sintomas emocionales entre un grupo de practicantes en psicologia clinica novatos comparados con una cohorte control. Se reclutaron 575 estudiantes de Psicologia colombianos: 52.9% comenzaban su practica clinica y 47.1% cursaban un semestre normal. Al inicio del semestre (T1), los participantes respondieron a medidas de sintomas emocionales (Depression, Anxiety, and Stress Scale-21, DASS-21; General Health Questionnaire-12, GHQ-12). Aproximadamente dos meses despues (T2), respondieron al DASS-21 y GHQ-12. Los dos grupos no difirieron en las puntuaciones del DASS-21 y GHQ-12 en el T1. Los ANOVA de medidas repetidas Bayesianos mostraron que los practicantes incrementaron sus puntuaciones en el DASS-Total, DASS-Depresion y DASS-Estres mas que los participantes que asistian a un semestre normal. Este es el primer estudio que muestra que los practicantes de psicologia clinica experimentan un mayor incremento de sintomas emocionales que los de una cohorte control. EnglishAbstract Cross-sectional research has shown that clinical psychology trainees usually face a wide range of stressors related to the clinical practice and tend to present higher distress than other psychology students. However, to our knowledge, no longitudinal study has been conducted analyzing this fact. Specifically, this study analyses the evolution of emotional symptoms among a group of novice clinical psychology trainees compared with a control cohort. We recruited 575 Colombian Psychology undergraduates: 52.9% were in the semester in which they began their clinical practice, and 47.1% were attending a regular semester. At the beginning of the semester (T1), participants responded to measures of emotional symptoms (Depression, Anxiety, and Stress Scale - 21, DASS-21; General Health Questionnaire - 12, GHQ-12). Approximately two months later (T2), participants responded to the DASS-21 and GHQ-12. The two groups did not differ in their scores on the DASS-21 and the GHQ-12 at T1. Bayesian repeated-measures ANOVA showed that clinical psychology trainees showed higher increases in scores on the DASS-Total, DASS-Depression, and DASS-Stress than participants attending a regular semester. This is the first study that shows clinical psychology trainees experiencing a higher increase in emotional symptoms compared with a control cohort.
Impact of RNA Extraction and Target Capture Methods on RNA Sequencing Using Formalin-Fixed, Paraffin Embedded Tissues

Formalin fixed paraffin embedded (FFPE) tissues are commonly used biospecimen for clinical diagnosis. However, RNA degradation is extensive when isolated from FFPE blocks making it challenging for whole transcriptome profiling (RNA-seq). Here, we examined RNA isolation methods, quality metrics, and the performance of RNA-seq using different approaches with RNA isolated from FFPE and fresh frozen (FF) tissues. We evaluated FFPE RNA extraction methods using six different tissues and five different methods. The reproducibility and quality of the prepared libraries from these RNAs were assessed by RNA-seq. We next examined the performance and reproducibility of RNA-seq for gene expression profiling with FFPE and FF samples using targeted (Kinome capture) and whole transcriptome capture based sequencing. Finally, we assessed Agilent SureSelect All-Exon V6+UTR capture and the Illumina TruSeq RNA Access protocols for their ability to detect known gene fusions in FFPE RNA samples. Although the overall yield of RNA varied among extraction methods, gene expression profiles generated by RNA-seq were highly correlated (>90%) when the input RNA was of sufficient quality (≥DV200 30%) and quantity (≥ 100 ng). Using gene capture, we observed a linear relationship between gene expression levels for shared genes that were captured using either All-Exon or Kinome kits. Gene expression correlations between the two capture-based approaches were similar using RNA from FFPE and FF samples. However, TruSeq RNA Access protocol provided significantly higher exon and junction reads when compared to the SureSelect All-Exon capture kit and was more sensitive for fusion gene detection. Our study established pre and post library construction QC parameters that are essential to reproducible RNA-seq profiling using FFPE samples. We show that gene capture based NGS sequencing is an efficient and highly reproducible strategy for gene expression measurements as well as fusion gene detection.
Can brain natriuretic peptide predict cardiovascular complications in severe preeclampsia? A case-control study

Abstract Background Preeclampsia is one of the most common pregnancy complications, which is one of the major causes of fetal and maternal mortality. Objective To compare the serum Brain Natriuretic Peptide (BNP) level in patients with severe preeclampsia and normal pregnancy and investigate associated cardiovascular complications. Materials and Methods This case-control study was performed on 94 women with a singleton pregnancy (severe preeclampsia and normal pregnancy) at Imam Reza Hospital in Mashhad, Iran. The venous blood samples were collected to evaluate the serum BNP level. All patients were subjected to echocardiography performed by a single cardiologist. Results The mean pro-BNP levels were 480.36 ± 754.52 and 67.46 ± 106.24 pg/dL in the severe preeclampsia and normal pregnancy patients, respectively (p < 0.001). However, adjusted BNP for maternal and gestational age was not different in the two groups (ANCOVA, p = 0.69). Furthermore, the two study groups showed no significant difference in terms of the cardiac parameters, including ejection fraction (EF), left ventricle end-diastolic and -systolic diameters (LVEDD and LVESD, respectively), tricuspid annular plane systolic excursion (TAPSE), and ratio of the early transmitral flow velocity to the early diastolic tissue velocity (E/Em). However, the serum BNP level showed a significant correlation with EF (r = -0.39, p = 0.008), TAPSE (r = -0.47, p = 0.001), and E/Em ratio (r = 0.48, p = 0.001) in patients with severe preeclampsia. Conclusion It seems that BNP can be used as a predictor for some of the main cardiac functional indices (i.e., E/Em, EF, and TAPSE) in severe preeclampsia patients.
An uncommon cause of isolated ascites: Pseudomyxoma peritonei

Pseudomyxoma peritonei or Gelatinous Peritoneal Disease is a rare disease. We report a case treated in the department of Hepato-Gastroenterology at Ibn Sina Hospital in Rabat, of a 64-year-old male who presented with an abdominal pain and an increased volume of the abdomen corresponding to ascites. Imaging and anatomopathological study made it possible to diagnose the disease. However, given the general state of the patient, he is under palliative care. Case Report
Diversity in antioxidant properties and mineral contents of Allium paradoxum in the Hyrcanian forests, Northern Iran

Khodadadi S, Nejadsattari T, Naqinezhad A, Ebrahimzadeh MA. 2015. Diversity in antioxidant properties and mineralcontents of Allium paradoxum in the Hyrcanian forests, Northern Iran. Biodiversitas 16: 281-287. The knowledge about variation ofantioxidant properties from local medicinal plant can be achieved by investigating all natural habitats of it. To reach this goal, acomprehensive survey on eight populations of Allium paradoxum (M. B.) G. Don, was conducted in the Hyrcanian forests. A.paradoxum (Amaryllidaceae) is a perennial, local plant, native to the northern Iran. Different parts of it, is largely used in foodpreparation and traditional medicine. Plants were collected randomly from different altitudes and forest sites of Iranian northernprovinces ranging from west to east (Guilan, Mazandaran and Golestan Provinces). Samples were divided into aerial and bulbous parts.The antioxidant activities of the extracts were investigated with 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power assays.Total flavonoid content was determined by a colorimetric aluminum chloride method. The highest antioxidant activities and flavonoidcontents in A. paradoxum were related to Varaki and Zarinabad sites (Mazandaran province) with relatively higher humidity, in thecentral part of the Hyrcanian area and in altitudinal range between 462-860 m asl. The content of total phenolics in the extracts wasdetermined according to the Folin-Ciocalteu reagent method, and calculated as gallic acid equivalents (GAE). With respect to totalphenolic contents, aerial parts of plants of Jahannama, the lowest elevation site (Golestan province), had the highest amount. Elementalcomposition (Fe++, Mn++) and total sulphur were also determined using Atomic Absorption Spectroscopy and digestion method,respectively. Higher contents of two elements, particularly Fe, and total sulphur can be found in bulbous part of Zarinabad and Kiasarpopulations (Mazandaran province) compared to other sites. The results of the current study indicated that there is a remarkable andsignificant variation of antioxidant activities among different studied populations of A. paradoxum in the Hyrcanian forests.Key words: Allium paradoxum, antioxidant activity, medicinal plant, mineral contents
Baseline tumor-immune signatures associated with response to bempegaldesleukin (NKTR-214) and nivolumab.

2623 Background: PIVOT-02 is an ongoing phase 1/2 study of bempegaldesleukin (NKTR-214), a CD122-preferential IL-2 pathway agonist, plus nivolumab in patients with advanced solid tumors. Bempegaldesleukin (NKTR-214) increases proliferative tumor infiltrating lymphocytes (TIL) and cell surface PD-1 on immune cells and PD-L1 on tumor cells, demonstrating potential synergy with anti-PD-1 therapy. Pre-treatment tumor biopsies from metastatic 1L melanoma (MEL) and urothelial carcinoma (UC) patients were analyzed to correlate baseline immune phenotype to response. Methods: Pre-treatment TIL (CD8+ T cells/mm2 and %CD3+ by IHC; 29 MEL; 22 UC) were measured and divided into high and low groups based on median values. PD-L1 (% PD-L1 on tumor cells by IHC [28-8 PharmDx]; 33 MEL; 23 UC) was scored negative (<1%) or positive (≥1%). Interferon gamma gene score (IFNG; 11 MEL) was scored as high or low based on median p value of <0.1 for 15 genes (EdgeSeq). High and low groups were correlated with responses per RECIST 1.1. Results: Baseline demographics and prognostic factors were balanced in the biomarker subgroups. Response rates for response evaluable MEL and UC were 53% (SITC 2018) and 48% (ASCO-GU 2019), respectively. In MEL, median values of CD3-TIL and CD8-TIL were 19% and 203 cells/mm2, respectively. Response rate correlations were 67% and 20% with IFNG high and low, 79% and 29% with CD3-TIL high and low, 79% and 33% with CD8-TIL high and low, and 68% and 43% with PD-L1 positive and negative. Most importantly, responses were observed in patients with the least favorable tumor microenvironment, characterized as both PD-L1 negative and TIL low, with responses of 17% (1/6 CD8-TIL), and 25% (2/8 CD3-TIL), respectively. Similar correlative trends were observed in UC, with 50% (4/8 CD8-TIL) and 38% (3/8 CD3-TIL) responses in patients with least favorable microenvironment. Conclusions: The biomarker program included in PIVOT-02 identified baseline immune signatures correlated with response for MEL and UC. The response rates observed in both the favorable and unfavorable tumor microenvironments indicate the potential of this combination and support its broad development. Clinical trial information: NCT02983045.
Inhibition of N822K T>A mutation-induced constitutive c-KIT activation in AML cells triggers apoptotic and autophagic pathways leading to death

Background: The D816V mutation of c-KIT can constitutively activate tyrosine kinase, thereby promote core binding factor acute myeloid leukemia (CBF-AML) cell proliferation and inhibit apoptosis. Previous studies have indicated similar proliferation and apoptosis between N822K and D816V mutations.The current study aims to determine the occurrence and potential functions of N822K mutation-induced c-KIT activation in AML cells, and explore possible mechanisms of poor prognosis of CBF-AML. Methods: c-KIT N822K mutation status in AML cells was determined by exon 17 sequencing. The level of c-KIT expression was detected by flow cytometry (FCM) and colony formation was assessed after hu-SCF stimulation. After exposure to sunitinib (a kind of tyrosine kinase inhibitor, TKI), cell proliferation inhibition was tested by MTT, cell cycle and apoptosis were measured by FCM, autophagy was assessed by fluorescence microscopy and immunoblotting. Results: Kasumi-1 cell line was detected to bear c-KIT N822K (T>A) mutation. After hu-SCF stimulation, CD117 expression was decreased and the colony formation efficiency was not altered in Kasumi-1 cells. After sunitinib inhibited the c-KIT activity, the colony formation efficiency was reduced, and the half-maximal inhibitory concentration (IC50) of sunitinib was low (0.44±0.17μM) at 48 hours. Moreover, cells were arrested in G0/G1 phase, corresponding to an increase of apoptosis ratio. Acidic vesicular organelles (AVO) were observed along with an altered expression of autophagy-related proteins in Kasumi-1 cells. Conclusions: Our data indicated that inhibition of N822K T>A mutation-induced constitutive c-KIT activation in AML cells triggered apoptotic and autophagic pathways leading to death, and c-KIT N822K mutation may have clinical application as a CBF-AML treatment target.
Predictive value of crp/albumin ratio for in-hospital atrial fibrillation development in st segment elevation myocardial infarction

Aim: Atrial fibrillation(AF) development in ST elevation myocardial infarction (STEMI) patients is associated with increased cardiovascular mortality and morbidity. The CRP to Albumin ratio(CAR) is a new described parameter and it has been suggested that this parameter may also be correlated with cardiovascular diseases. In this study, we investigated the association between AF development and the CAR in STEMI patients undergoing percutaneous coronary intervention. Material and Methods: This study was conducted in patients presenting to our department with a diagnosis of STEMI retrospectively and undergoing percutaneous coronary intervention. A total of 110 patients were included in the study. The patients were divided into two groups according to AF development and the predictors for AF development were investigated. Results: The mean age of the patients was 62±5.1 years and 72% of them were males. While CAR, TIMI, CRP, albumin, neutrophil to lymphocyte to ratio, lesion localization, myocardial blush grade and syntax score were associated with the AF development in the univariate analysis, the CAR was determined to be an independent predictor of AF development in the multivariate regression analysis. Conclusions: We have demonstrated that the CAR was an independent predictor for prediction of AF development and we think that this parameter may be useful in clinical practice.
Associated clinical manifestations and self-management approaches of primary dysmenorrhea among adolescent students in Erbil City, Iraq

Background: Primary dysmenorrhea is one of the most common gynecological problems among adolescent females which interferes with daily activities and affects their physical and emotional aspects and has a negative impact on quality of life. Self-medication for primary dysmenorrhea is a very common practice. Objective: This study aimed to find the associated clinical manifestations and self-management approaches of primary dysmenorrhea and its relations with some demographical and menstrual characteristics. Materials and Methods: A descriptive cross-sectional study was conducted on 300 dysmenorrheic adolescent students in secondary schools in Erbil city, during the period from March to June 2016. A purposive (nonprobability) sampling was used. A questionnaire format was prepared by researcher which included following parts: sociodemographic data, menstrual data, clinical manifestation, and self-management approaches to minimize this problem. Results: Majority of the study samples aged between 14 and 17 years, their school class was 11, the education of their mothers was illiterate, and mothers' occupation was housewife. Regarding the menstrual characteristics, age of menarche among majority of the study samples was between 12 and 14 years, frequency of menstrual cycle was between 29 and 31 days, and level of pain during menstruation was severe. There was a high significant association between headache, breast tenderness, and age of adolescents. Significant association was also found between reduced ability of concentration and age of menarche. There was a significant relationship between self-management approach used by adolescents with school class and regularity of blood flow. Conclusion: Primary dysmenorrhea considerably affected physical activity and concentration of students. Self-management approaches practiced by adolescent participated in this study were poor.
Teaching, Learning and Research in Higher Education: A Critical Approach

Illustrations Preface 1. Introduction 2. Perspectives on Quality Teaching 3. Reconceptualising the Development of University Teaching Expertise 4. Framing Teacher-Learner Relationships 5. Learning Groups 6. Teaching for Diversity 7. Assessment 8. Promoting Workplace-Oriented Learning 9. Learning in the Digital Age 10. Postgraduate Research Education 11. Teaching and Research References Index
A Protein Kinase A-Regulated Network Encodes Short- and Long-Lived Cellular Memory

Cells can store memories of prior experiences to modulate their responses to subsequent stresses, as seen for the protein kinase A (PKA)-mediated general stress response in yeast, which is required for resistance against future stressful conditions. Using microfluidics and time-lapse microscopy, we quantitatively analyzed how cellular memory of stress adaptation is encoded in single yeast cells. We found that cellular memory is biphasic. Short-lived memory was mediated by trehalose synthase and trehalose metabolism. Long-lived memory was mediated by PKA-regulated stress-responsive transcription factors and cytoplasmic messenger ribonucleoprotein (mRNP) granules. Strikingly, short- and long-lived memory could be selectively induced by different priming input dynamics. Computational modeling revealed how the PKA-mediated regulatory network could encode previous stimuli into memories with distinct dynamics. This biphasic memory-encoding scheme, analogous to innate and adaptive immune responses in mammals, might represent a general strategy to prepare for future challenges in rapidly changing environments.
An Overview of the Pathogenesis and Treatment of Elbow Osteoarthritis

The elbow joint could be associated with degenerative processes of primary and post-traumatic aetiology. Among these, osteoarthritis may also be secondary to repeated use as well as trauma. Pain, discomfort and progressive loss of functionality are common signs of this condition. The evaluation of elbow osteoarthritis should comprise an in-depth study to detect the primary cause of the illness and to facilitate the decision-making process regarding personalized treatment. Discordance between clinical manifestations and radiological findings is common. Conservative approaches may provide symptomatic relief in the early stages of disease for most patients. The goal of the treatment is to reduce pain and ensure an adequate range of motion and proper functioning of the joint while preserving the anatomical structure, to postpone elbow arthroplasty interventions for as long as possible. According to treatment guidelines, surgery should be considered depending on aetiology and severity, patient age, and functional demands. This narrative review aims to investigate the current literature regarding the pathogenesis and treatment of primary and post-traumatic arthritis of the elbow.
Influence of Phosphorus Fertilizer Rates on Yield and Yield Components of Faba Bean (Vicia faba L.) Varieties in Lemu Bilbilo District of Arsi Zone, Southeastern Ethiopia

Faba bean production is a common practice in Lemu Bilbilo areas. However, faba bean productivity is affected and limited by poor soil fertility and lack of alternative technologies such as application of optimum phosphorus fertilizer for different faba bean varieties. In view of this, a field experiment was conducted on farmer’s field during the 2017 main cropping season at Lemu Bilbilo with the objectives to determine the response of faba bean varieties to different rates of P fertilizer and its influence on yield and yield components of faba bean varieties. The treatments include three faba bean varieties (Tumsa, Gebelcho and Dosha) and five phosphorus levels (0, 10, 20, 30 and 40 kg P ha-1) from Triple Super Phosphate. The experiment was laid out in a randomized complete blocked design with 3 x 5 factorial arrangements with three replications. Significantly (P<0 .05) higher plant height was recorded from Tumsa variety. Total productive tillers per plant, thousand seed weights, and harvest index and biomass yield of faba bean were significantly affected by main effect of varieties. Higher total productive tillers per plant (1.53) were obtained from application of 30 kg P ha-1. Higher plant height (153 cm), biomass yield (14158 kg ha-1) and grain yield (6323 kg ha-1) were obtained from application of 40 kg P ha-1. Application of 10, 20 and 30 kg P ha-1 gave marginal rate of return of 1404694 and 502% for faba bean production, which are well above the minimum acceptable rate of return. Therefore, application of 20 kg ha-1 of P with Tumsa, Gebelcho and Dosha faba bean varieties were proved to be productive and superior both in seed yield as well as economic advantage and recommended for faba bean production in Lemu Bilbilo area. Further study should be conducted in the future both over locations and years in order to give full recommendation for practical application.
Use of Ambulance Dispatch Calls for Surveillance of Severe Acute Respiratory Infections

Ambulance dispatches for respiratory syndromes reflect incidence of influenza-like illness in primary care. Associations are highest in children (15%–34% of respiratory calls attributable to influenza), out-of-office hours (9%), and highest urgency-level calls (9%–11%). Ambulance dispatches might be an additional source of data for severe influenza surveillance.