Miracle-dz/UniFAR-FTUs huggingface dataset:
Mast Cells Involvement in the Inflammation and Fibrosis Development of the TNBS-induced Rat Model of Colitis

Background: Mast cells have been implicated in chronic inflammatory conditions resulting in fibrosis, such as Crohn disease. However, a link between inflammation, fibrosis and mast cells has not been demonstrated in human or animal intestinal diseases. This work was undertaken to analyze whether mast cells play a role in inflammation and fibrosis in the TNBS-induced rat colitis. Methods: Rats were rectally instilled 2,4,6,-trinitrobenzene sulfonic acid in ethanol, and immediately or 4 days later injected daily i.p. with nedocromil sodium, a mast cell stabilizer, compound 48/80, a mast cell activator, or saline. Rats were sacrificed 5 days post-TNBS, or on day 21. Intestinal inflammation and fibrosis were assessed by gross and histopathological evaluation. Colonic mast cell numbers (toluidine blue) and collagen (type I mRNA expression) were evaluated. Mast cell sonicate was added to rat colon fibroblasts. Fibroblast proliferation ( 3 H-thymidine), collagen synthesis ( 3 H-proline) and contractile activity (tridimensional collagen lattice contraction) were then assessed. Results: Nedocromil reduced inflammation and fibrosis possibly by decreasing mast cell numbers and activation and consequent collagen production. Compound 48/80 slightly enhanced the severity of the disease by activating mast cells. Mast cells increased fibroblast proliferation, collagen production and contractile activity. Conclusions: Mast cells are involved in the gastrointestinal tract inflammation and fibrosis of the TNBS-colitis rats.
Presence and severity of intensive care unit-acquired paresis at time of awakening are associated with increased intensive care unit and hospital mortality*

Objectives: To assess whether the presence and severity of intensive care unit-acquired paresis are associated with intensive care unit and in-hospital mortality. Design: Prospective, observational study. Setting: Two medical, one surgical, and one medico-surgical intensive care units in two university hospitals and one university-affiliated hospital. Patients: A total of 115 consecutive patients were enrolled after > 7 days of mechanical ventilation. Interventions: None. Measurements and Main Results: The Medical Research Council score (from 0–60) was used to evaluate upper and lower limb strength at time of awakening, identified as the ability to follow five commands. Intensive care unit-acquired paresis was defined as a Medical Research Council score <48. Patients were followed-up until hospital discharge. The primary end point was hospital mortality. At awakening, median Medical Research Council score was 41 (interquartile range, 21–52), and 75 (65%) patients had intensive care unit-acquired paresis. Hospital non-survivors had a significantly lower Medical Research Council score at awakening (21 [11–43]) vs. 41 [28–53]; p = . 008) and a significantly higher rate of intensive care unit-acquired paresis (85.1% vs. 58.4%; p = .02) compared to survivors. After multivariate risk adjustment, intensive care unit-acquired paresis was independently associated with higher hospital and intensive care unit mortality (odds ratio for hospital mortality, 2.02; 95% confidence interval, 1.03–8.03; p = .048). Each Medical Research Council point decrease was associated with a significantly higher hospital mortality (odds ratio, 1.03; 95% confidence interval, 1.01–1.05; p = .033). Conclusions: Both the presence and severity of intensive care unit-acquired paresis at the time of awakening are associated with increased intensive care unit and hospital mortality; the mechanisms underlying this association need further study.
Tryptophan Scanning Reveals Dense Packing of Connexin Transmembrane Domains in Gap Junction Channels Composed of Connexin32*

Background: Transmembrane domain interactions in gap junction channels are poorly understood. Results: Tryptophan substitution experiments involving all four TM domains of Cx32 revealed tight packing. Conclusion: After modeling, tight packing was found to occur in the midregion. Pore-facing residues were highly sensitive to substitution, whereas lipid-facing residues were variably tolerant. Significance: Connexin-based channels are more densely packed than their innexin-based counterparts. Tryptophan was substituted for residues in all four transmembrane domains of connexin32. Function was assayed using dual cell two-electrode voltage clamp after expression in Xenopus oocytes. Tryptophan substitution was poorly tolerated in all domains, with the greatest impact in TM1 and TM4. For instance, in TM1, 15 substitutions were made, six abolished coupling and five others significantly reduced function. Only TM2 and TM3 included a distinct helical face that lacked sensitivity to tryptophan substitution. Results were visualized on a comparative model of Cx32 hemichannel. In this model, a region midway through the membrane appears highly sensitive to tryptophan substitution and includes residues Arg-32, Ile-33, Met-34, and Val-35. In the modeled channel, pore-facing regions of TM1 and TM2 were highly sensitive to tryptophan substitution, whereas the lipid-facing regions of TM3 and TM4 were variably tolerant. Residues facing a putative intracellular water pocket (the IC pocket) were also highly sensitive to tryptophan substitution. Although future studies will be required to separate trafficking-defective mutants from those that alter channel function, a subset of interactions important for voltage gating was identified. Interactions important for voltage gating occurred mainly in the mid-region of the channel and focused on TM1. To determine whether results could be extrapolated to other connexins, TM1 of Cx43 was scanned revealing similar but not identical sensitivity to TM1 of Cx32.
Bilateral somatosensory evoked potentials in four patients with long‐standing surgical hemispherectomy

Four patients were studied electrophysiologically 8 to 24 years after surgical removal of one cerebral hemisphere without damage to the striatum or diencephalon. Somatosensory evoked potentials(SEPs)to electrical stimulation of the median nerve on the left or right side were averaged and mapped out over the scalp. Stimulation on the side opposite to the missing hemisphere evoked brief P9 and P14 farfields and a slow N18 negative potential of 15‐to 25‐msec duration bilaterally. No additional focal response was detected over the remaining (ipsilateral) hemisphere for 60 msec after the stimulus. Because long‐standing hemispherectomy entails massive retrograde degeneration of thalamocortical neurons, the preserved P14 and N18 responses must reflect neural activities generated below the thalamus that are volume conducted to the scalp bilaterally. The data clarify several current issues in the evaluation of SEP components.
Economic Impact of Natural Disasters: An Empirical Re-examination

Natural disasters cause serious economic and human losses. Yet there remains ambiguity in the existing literature with regard to their impact on the economy at large. This study re-examines the relationship between natural disasters and economic growth. It aims to contribute to a fairly limited literature on the economy-wide and sector-specific consequences of natural disasters in the short-to-medium term (up to 5 years). Further, it examines whether the disaster impacts are dependent on a country’s level of development. Based on panel data consisting of 102 (29 developed and 73 developing) countries over the period 1981–2015, it looks at the growth effects of four types of natural disasters, namely, floods, droughts, storms and earthquakes that were explored using the system generalised method of moments (GMM) approach. The results indicate that natural disasters have diverse economic impacts across economic sectors depending on disaster types and their intensity. The study confirms the findings of previous studies that the economic impacts of natural disasters are statistically stronger in developing countries. These findings may stimulate the policymakers especially in developing countries to explore the efficacy of viable ex-ante disaster risk financing tools (such as insurance, micro-insurance and catastrophic bonds). This would not only safeguard population and physical assets but also ensure adherence to the sustainable development goals. JEL Classification: Q54, O10
Staphylococcus aureus Leukotoxin GH Promotes Formation of Neutrophil Extracellular Traps

Staphylococcus aureus secretes numerous virulence factors that facilitate evasion of the host immune system. Among these molecules are pore-forming cytolytic toxins, including Panton–Valentine leukocidin (PVL), leukotoxin GH (LukGH; also known as LukAB), leukotoxin DE, and γ-hemolysin. PVL and LukGH have potent cytolytic activity in vitro, and both toxins are proinflammatory in vivo. Although progress has been made toward elucidating the role of these toxins in S. aureus virulence, our understanding of the mechanisms that underlie the proinflammatory capacity of these toxins, as well as the associated host response toward them, is incomplete. To address this deficiency in knowledge, we assessed the ability of LukGH to prime human PMNs for enhanced bactericidal activity and further investigated the impact of the toxin on neutrophil function. We found that, unlike PVL, LukGH did not prime human neutrophils for increased production of reactive oxygen species nor did it enhance binding and/or uptake of S. aureus. Unexpectedly, LukGH promoted the release of neutrophil extracellular traps (NETs), which, in turn, ensnared but did not kill S. aureus. Furthermore, we found that electropermeabilization of human neutrophils, used as a separate means to create pores in the neutrophil plasma membrane, similarly induced formation of NETs, a finding consistent with the notion that NETs can form during nonspecific cytolysis. We propose that the ability of LukGH to promote formation of NETs contributes to the inflammatory response and host defense against S. aureus infection.
Detection and modelling of case clusters for urban leptospirosis

Objective To analyse the epidemiological profile of 488 cases of leptospirosis in Rio de Janeiro, Brazil between 1997 and 2002, using a variety of methods of spatial epidemiology, to establish alert guidelines in general hospitals, which might be a tool to improve diagnosis and treatment of leptospirosis to reduce lethality rates.
The Rise of Private Foundations as Owners of Swedish Industry: The Role of Tax Incentives 1862–2018

The tax system has at times favoured firm control through private foundations, which has been argued to inhibit high-impact entrepreneurship and economic growth. However, research has been hampered due to a lack of systematic historical tax data. The purpose of this study is threefold. First, we describe the evolution of tax rules for private foundations in Sweden between 1862 and 2018. Second, we calculate the marginal effective tax rate on capital income. Third, we examine the incentives to use private foundations as a means for corporate control by comparing the taxation of private foundations and of high-impact entrepreneurs. Tax incentives help explain why economically significant private foundations were founded between World War I and the 1960s.
Hairpin Primers for Simplified Single-Nucleotide Polymorphism Analysis of Mycobacterium tuberculosis and Other Organisms

ABSTRACT We describe a novel, simple, rapid, and highly sensitive method to detect single-nucleotide polymorphisms (SNPs) in Mycobacterium tuberculosis and other organisms. Amplification refractory mutation (ARMS) SNP assays were modified by converting the SNP-detecting linear primers in the ARMS assay to hairpin-shaped primers (HPs) through the addition of a 5′ tail complementary to the 3′ end of the linear primer. The improved ability of these primers to detect SNPs in M. tuberculosis was compared in a real-time PCR with SYBR-I green dye. Linear primers resulted in incorrect or indeterminate allele designation for 6 of the 13 SNP alleles tested in seven different SNP assays, while HPs determined the correct SNP in all cases. We compared the cycle threshold differences (ΔCt) between the reactions containing primer-template matches and the reactions containing primer-template mismatches (where a larger ΔCt indicates a more robust assay). The use of HPs dramatically improved the mean ΔCt values for the SNP assays (7.6 for linear primers and 11.2 for HPs). We designed 98 different HP assays for SNPs previously associated with resistance to the antibiotic isoniazid to test the large-scale utility of the HP approach. Assay design was successful in 72.4%, 83.7%, 88.8%, and 92.9% of the assays after one to four rounds of assay design, respectively. HP SNP assays are simple, sensitive, robust, and inexpensive. These advantages favor the application of this technique for SNP assays of M. tuberculosis and other organisms.
Development and Validation of the Total HUman Model for Safety (THUMS) Toward Further Understanding of Occupant Injury Mechanisms in Precrash and During Crash

Objective: Active safety devices such as automatic emergency brake (AEB) and precrash seat belt have the potential to accomplish further reduction in the number of the fatalities due to automotive accidents. However, their effectiveness should be investigated by more accurate estimations of their interaction with human bodies. Computational human body models are suitable for investigation, especially considering muscular tone effects on occupant motions and injury outcomes. However, the conventional modeling approaches such as multibody models and detailed finite element (FE) models have advantages and disadvantages in computational costs and injury predictions considering muscular tone effects. The objective of this study is to develop and validate a human body FE model with whole body muscles, which can be used for the detailed investigation of interaction between human bodies and vehicular structures including some safety devices precrash and during a crash with relatively low computational costs. Methods: In this study, we developed a human body FE model called THUMS (Total HUman Model for Safety) with a body size of 50th percentile adult male (AM50) and a sitting posture. The model has anatomical structures of bones, ligaments, muscles, brain, and internal organs. The total number of elements is 281,260, which would realize relatively low computational costs. Deformable material models were assigned to all body parts. The muscle–tendon complexes were modeled by truss elements with Hill-type muscle material and seat belt elements with tension-only material. The THUMS was validated against 35 series of cadaver or volunteer test data on frontal, lateral, and rear impacts. Model validations for 15 series of cadaver test data associated with frontal impacts are presented in this article. The THUMS with a vehicle sled model was applied to investigate effects of muscle activations on occupant kinematics and injury outcomes in specific frontal impact situations with AEB. Results and Conclusions: In the validations using 5 series of cadaver test data, force–time curves predicted by the THUMS were quantitatively evaluated using correlation and analysis (CORA), which showed good or acceptable agreement with cadaver test data in most cases. The investigation of muscular effects showed that muscle activation levels and timing had significant effects on occupant kinematics and injury outcomes. Although further studies on accident injury reconstruction are needed, the THUMS has the potential for predictions of occupant kinematics and injury outcomes considering muscular tone effects with relatively low computational costs.
Fiscal rules in a monetary economy: Implications for growth and welfare

This study considers two fiscal rules, a debt rule that controls the debt‐to‐gross domestic product (GDP) ratio, and an expenditure rule that controls the expenditure‐to‐GDP ratio, in a monetary growth model with financial intermediation. Tightening of fiscal rules promotes economic growth and thus, benefits future generations. However, there could be two equilibria of the nominal interest rates, and the welfare effects of the rules on the current generation are different between the two equilibria. In particular, the effects of a decreased debt‐to‐GDP ratio depend on its initial ratio; a high (low)‐ratio country has no incentive (an incentive) to reduce the ratio further from the viewpoint of the current generation's welfare. This result provides an explanation for difficulties with fiscal reform in countries with already high debt‐to‐GDP ratios.
Mutations Conferring Aminoglycoside and Spectinomycin Resistance in Borrelia burgdorferi

ABSTRACT We have isolated and characterized in vitro mutants of the Lyme disease agent Borrelia burgdorferi that are resistant to spectinomycin, kanamycin, gentamicin, or streptomycin, antibiotics that target the small subunit of the ribosome. 16S rRNA mutations A1185G and C1186U, homologous to Escherichia coli nucleotides A1191 and C1192, conferred >2,200-fold and 1,300-fold resistance to spectinomycin, respectively. A 16S rRNA A1402G mutation, homologous to E. coli A1408, conferred >90-fold resistance to kanamycin and >240-fold resistance to gentamicin. Two mutations were identified in the gene for ribosomal protein S12, at a site homologous to E. coli residue Lys-87, in mutants selected in streptomycin. Substitutions at codon 88, K88R and K88E, conferred 7-fold resistance and 10-fold resistance, respectively, to streptomycin on B. burgdorferi. The 16S rRNA A1185G and C1186U mutations, associated with spectinomycin resistance, appeared in a population of B. burgdorferi parental strain B31 at a high frequency of 6 × 10−6. These spectinomycin-resistant mutants successfully competed with the wild-type strain during 100 generations of coculture in vitro. The aminoglycoside-resistant mutants appeared at a frequency of 3 × 10−9 to 1 ×10−7 in a population and were unable to compete with wild-type strain B31 after 100 generations. This is the first description of mutations in the B. burgdorferi ribosome that confer resistance to antibiotics. These results have implications for the evolution of antibiotic resistance, because the 16S rRNA mutations conferring spectinomycin resistance have no significant fitness cost in vitro, and for the development of new selectable markers.
Towards an Informative Mutant Phenotype for Every Bacterial Gene

ABSTRACT Mutant phenotypes provide strong clues to the functions of the underlying genes and could allow annotation of the millions of sequenced yet uncharacterized bacterial genes. However, it is not known how many genes have a phenotype under laboratory conditions, how many phenotypes are biologically interpretable for predicting gene function, and what experimental conditions are optimal to maximize the number of genes with a phenotype. To address these issues, we measured the mutant fitness of 1,586 genes of the ethanol-producing bacterium Zymomonas mobilis ZM4 across 492 diverse experiments and found statistically significant phenotypes for 89% of all assayed genes. Thus, in Z. mobilis, most genes have a functional consequence under laboratory conditions. We demonstrate that 41% of Z. mobilis genes have both a strong phenotype and a similar fitness pattern (cofitness) to another gene, and are therefore good candidates for functional annotation using mutant fitness. Among 502 poorly characterized Z. mobilis genes, we identified a significant cofitness relationship for 174. For 57 of these genes without a specific functional annotation, we found additional evidence to support the biological significance of these gene-gene associations, and in 33 instances, we were able to predict specific physiological or biochemical roles for the poorly characterized genes. Last, we identified a set of 79 diverse mutant fitness experiments in Z. mobilis that are nearly as biologically informative as the entire set of 492 experiments. Therefore, our work provides a blueprint for the functional annotation of diverse bacteria using mutant fitness.
Accounting for gender : a historical evaluation of the gendered hierarchy of the UK accounting profession

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Harmonic 3D shape matching

With the advent of the world wide web, the number of available 3D models has increased substantially and the challenge has changed from "How do we generate 3D models? " to "How do we find them? " In this sketch we describe a new 3D model matching and indexing algorithm that uses spherical harmonics to compute discriminating similarity measures without requiring repair of model degeneracies or alignment of orientations. It provides 46-245% better performance than related shape matching methods during precision-recall experiments, and it is fast enough to return query results from a repository of 20,000 models in under half a second.
Predicting body mass index in women: The value of the psychological components of depression, anxiety, dietary restraint, and nutritional habits

Background: Understanding the association between psychological affective disturbances and anthropometric parameters, including body mass index (BMI), is important. These issues may have potential preventive effects on weight reduction in relation to different aspects of women's lifestyles and psychopathological states. The present study aimed to predict BMI based on psychological factors including; depression, anxiety, dietary restraint, and nutritional habits, in a sample of women with sedentary jobs in several Iranian governmental organizations. Methods: Two hundred consecutive women aged over 25 years, working on sedentary or low standing works such as banker, teachers, and employee in the social security organizations in Isfahan, Iran, were entered the study. To assess the severity of depression and anxiety symptoms, the Beck Depression Inventory II and the State-Trait Anxiety Inventory were used, respectively. To assess nutritional habits, a self-administered questionnaire was designed, and to evaluate dietary restraint status, the Ruderman questionnaire was used. To find the co-relationship between BMI and each of the psychological components, Pearson's correlation coefficient test was applied. Results: To assess the relationship between BMI and each of the psychological components, a multivariate regression model was used. Only two components of nutritional habits (b = −0.19, P < 0.001) and dietary restraint (b = 0.51, P < 0.001) could effectively predict BMI in Iranian women; while depression and anxiety components had low predictive values for predicting BMI. In total, these four variables could predict 34% of the variance of the dependent indicator (BMI). Conclusion: Nutritional habits and dietary restraint have high value for predicting BMI status in women aged more than 25 years working in sedentary jobs, while BMI status could not be predicted by assessing depression or anxiety severity.