Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional and T Cell Receptor Repertoire Analyses of Peripheral Blood and Infrapatellar Fat Pad T Cells in Knee Osteoarthritis

Objective. Osteoarthritis (OA) is a condition that features inflammation and immune responses of innate and adaptive immunity. The role of T cells in knee OA pathogenesis is still unclear. Our aim was to characterize T cell functions and their clonality in patients with knee OA in peripheral blood (PB) and infrapatellar fat pads (IPFP). Methods. We isolated T cells from PB and IPFP of patients with knee OA and PB of healthy individuals and determined soluble mediators produced from these cells. In addition, we performed a clonal analysis of activated CD8+ T cells and compared the T cell receptor β-variable gene chain (TRBV) usages between T cells in PB and IPFP of patients with knee OA. Results. Our results suggest that in patients with knee OA, circulating T cells possess a more “cytotoxic” profile or rather impaired cytokine production, but the knee microenvironment allows for these T cells to produce proinflammatory cytokines [interleukin (IL)-1β, IL-6, tumor necrosis factor], IL-17, and interferon-γ within IPFP. Activated CD8+ IPFP T cells carry different repertoire distribution from those present in PB of patients with knee OA. Shared TRBV usage of activated CD8+ IPFP T cells among the 3 patients with knee OA was also observed. Conclusion. Our study describes the nature of T cells in knee OA that may be due to “unhealthy” aging or other factors that drive healthy aging T cells into a state of imbalance, thus contributing to the pathogenesis of knee OA.
Using the Lymph2Cx assay for assessing cell-of-origin subtypes of HIV-related diffuse large B-cell lymphoma

Maria Joao Baptista , Gustavo Tapia, Mireia Morgades, Josep Muncunill, Ana-Mar ıa Mu~ noz-Marmol, Silvia Montoto, John G. Gribben, Maria Calaminici, Antonio Martinez , Blanca Gonzalez-Farre, Ivan Dlouhy, Eva Gonz alez-Barca, Mar ıa-Jos e Terol, Pilar Miralles, Miguel Alcoceba, Ferran Vall-Llovera, Javier Briones, Pau Abrisqueta, Eugenia Abella, Mariano Provencio, Carlos Garc ıa-Ballesteros, Jos e-Mar ıa Moraleda , Juan-Manuel Sancho, Josep-Maria Ribera , Jos e-Luis Mate and Jos e-Tomas Navarro Department of Hematology, ICO-Hospital Germans Trias i Pujol, Josep Carreras Leukaemia Research Institute (IJC), Institut Germans Trias i Pujol (IGTP), Universitat Aut onoma de Barcelona, Badalona, Spain; Department of Pathology, Hospital Germans Trias i Pujol, Universitat Aut onoma de Barcelona, Badalona, Spain; Centre for Haemato-Oncology, Barts Cancer Institute, Queen Mary University of London, London, United Kingdom; Department of Pathology, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain; Department of Hematology, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain; Department of Hematology, ICOHospital Duran i Reynals, L’Hospitalet de Llobregat, Spain; Department of Hematology and Oncology, Hospital Cl ınic Universitari de Val encia, Valencia, Spain; Department of Infectious Diseases, Hospital Gregorio Mara~ n on, Madrid, Spain; Department of Hematology, Hospital Universitario de Salamanca, Salamanca, Spain; Department of Clinical Hematology, Hospital Universitari Mut ua de Terrassa, Terrassa, Spain; Department of Hematology, Hospital de la Santa Creu i Sant Pau, Josep Carreras Leukaemia Research Institute (IJC), Barcelona, Spain; Department of Hematology, Hospital Vall d’Hebr on, Barcelona, Spain; Department of Hematology, Hospital del Mar, Barcelona, Spain; Department of Medical Oncology, Hospital Universitario Puerta De Hierro, Majadahonda, Spain; Department of Hematology, Hospital Arnau de Vilanova, Valencia, Spain; Department of Hematology, Hospital Clinico Universitario Virgen de la Arrixaca, Murcia, Spain
Resistance Status of Busseola fusca (Lepidoptera: Noctuidae) Populations to Single- and Stacked-Gene Bt Maize in South Africa

Abstract Transgenic Bt maize expressing Cry insecticidal δ-endotoxins of Bacillus thuringiensis has been cultivated in South Africa for the control of Busseola fusca since 98. Busseola fusca is resistant to Cry1Ab Bt maize at many localities throughout the maize production region. Pre-release evaluation (94–96) of the inherent susceptibility and post-release assessments (98–2011) of resistance status of B. fusca focused on a limited number of pest populations. This study reports the current levels of susceptibility of 10 B. fusca populations evaluated between 2013 and 2017 and compared this data with previously reported data on the survival of this pest on Bt maize, including data of pre-release evaluations done during 94 and 95. Larval feeding bioassays in which plant tissue of maize events expressing either Cry1Ab or Cry1A.105+Cry2Ab2 (stacked event) proteins were conducted and survival and different life history parameters recorded. Results show a shift in levels of susceptibility of B. fusca to Bt maize. Pre-release evaluation of the single-gene event showed very low larval survival on Bt maize leaf tissue while studies 10 yr later and the current study reported survival of up to 40% and 100% on Cry1Ab maize, respectively. While no larvae completed their life cycle on the stacked event, higher LT50 values in this study indicate a shift in susceptibility of B. fusca to the stacked-gene event and highlight the importance of baseline information and monitoring of pest populations for their susceptibility to Bt maize.
Long-term results of robotic anatomical segmentectomy for early-stage non-small-cell lung cancer

OBJECTIVES Anatomical segmentectomy is advocated for curative resection in select patients. We investigated the long-term results of robotic anatomical segmentectomy with mediastinal nodal dissection in patients with early-stage lung cancer. METHODS We retrospectively reviewed patients who underwent robotic anatomical segmentectomy for early-stage non-small-cell lung cancer (NSCLC). The follow-up data were obtained to determine survival and statistically significant risk factors in both univariable and multivariable models. RESULTS Seventy-one patients had clinical stage I NSCLC (36 men, 35 women, mean age 70 ± 12 years). All patients underwent R0 resection. The mean operating time was 134 min. Ten of 71 (14%) patients were upstaged. Eight of 71 (11%) patients were upstaged due to the size of tumour in the pathological specimen, and 2 of 71 (3%) patients were upstaged due to microscopic N2 nodal metastasis. Median hospitalization was 4 days (2-31 days). Complication rate was 29%. There were no complications attributable to the surgical robot. No patient died within 90 days. Mean follow-up was 54 months (range 2 months to 9 years). The overall 5-year survival was 43%, whereas lung cancer-specific 5-year survival was 55%. The 5-year lung cancer-specific survival for pathological stage I disease was 73%. Local or mediastinal recurrence occurred in 4 of 71(5%) patients. Pathological upstaging or recurrence resulted in 0% 5-year survival. The univariable and multivariable analyses showed that advanced age and pathological upstaging were statistically significant risk factors for lung cancer-specific death. CONCLUSIONS Robotic anatomical segmentectomy with mediastinal nodal dissection is a safe and feasible procedure. Accurate preoperative clinical staging is of critical importance for long-term survival.
Long noncoding RNA MEG3 play an important role in osteosarcoma development through sponging microRNAs

More and more evidence indicate long noncoding RNAs (lncRNAs) as competing endogenous RNAs (ceRNAs) to indirectly regulate messenger RNAs (mRNAs) by acting as microRNA (miRNA) sponges, which represents a novel layer of gene regulation that plays a critical role in the development of cancers. However, functional roles and regulatory mechanisms of lncRNA‐mediated ceRNAs network in osteosarcoma are still largely unknown. Here, we comprehensively compared the expression profiles of mRNAs, lncRNAs, and miRNAs between osteosarcoma and normal samples from the Gene Expression Omnibus (GEO) to elaborate related latent mechanisms. Two lncRNAs, ie, LINC01560 and MEG3, were identified to be aberrantly expressed. Importantly, MEG3 was considered as a promising diagnostic biomarker and therapeutic target for patients with osteosarcoma according to the Kaplan‐Meier analysis of another independent osteosarcoma data set from the Cancer Genome Atlas (P = 0.05). Eventually, we successfully established a dysregulated lncRNA‐related ceRNA network, including one osteosarcoma‐specific lncRNA, three miRNAs and four mRNAs. In conclusion, this study should be beneficial for improving our understanding of the lncRNA‐mediated ceRNA regulatory mechanisms in the pathogenesis of osteosarcoma and providing it with novel candidate diagnostic and therapeutic biomarkers.
Police response to individuals displaying suicidal or self‐harming behaviours: An integrative review

Police are increasingly being called upon to respond to people experiencing suicidal crisis. Such incidents are a challenging aspect of modern policing. This paper reports on an integrative review study that aimed to investigate police responses to individuals displaying suicidal or self-harming behaviours. Six electronic databases were searched for peer-reviewed articles published between 2007 and 2017 relating to police responses to individuals in suicidal crisis. The review identified 12 studies that met the inclusion criteria. A content analysis was conducted to identify and describe the key characteristics emerging from the literature, which identified four themes: "characteristics of individuals"; "the use of violence and weapons"; "contact with police prior to suicide"; and, "police officer training". Findings from this study indicate that individuals involved in incidents of suicidal and self-harm crisis with police are often male, aged between 35 and 40 years, single and/or having relationship issues, with a history of mental health issues and in recent contact with police prior to the incident of suicidal crisis-either as a victim or a perpetrator. The results highlight that large proportions of individuals in suicidal crisis within a community located incident are likely to present with violent or aggressive behaviour and in many situations are armed with a weapon used to either threaten or injure police and/or bystanders or self-harm. Training and education can have a positive impact from the perspective of police responding to individuals in suicidal crisis. Limitations in the current evidence are identified and implications for future research are outlined.
Speech delay in toddlers: Are they only `late talkers`?

Zengin-Akkuş P, Çelen-Yoldaş T, Kurtipek G, Özmert EN. Speech delay in toddlers: Are they only `late talkers`? Turk J Pediatr 2018; 60: 165-172. Speech delay is prevalent in toddlers. Although some children with speech delay are able to catch up with their peers with time, the delay might be part of a broader condition such as global developmental delay, which requires specific diagnostic work-up. The current study aimed to present the demographic features, developmental profiles of the first 100 preschool-aged children who were seen at Hacettepe University İhsan Doğramacı Children`s Hospital-Developmental Pediatrics Unit with parental concern of speech delay. Moreover, risk factors associated with speech delay, possible diagnosis and intervention strategies are documented. Thirty-one of 100 children were diagnosed with autism spectrum disorder (ASD) and global developmental delay (GDD). The current study presented that 4 out of 5 toddlers exceeded the recommended TV watching time. Moreover, almost one third of children had a poor linguistic home environment. Three parents whose children were diagnosed with ASD or GDD, refused to be reported as `autism spectrum disorder` or `intellectual disability` with the anxiety of labeling their child. As a result, these children were unable to receive special education paid for by the government. This study presented 31 of 100 children needed further diagnostic work up and early intervention. Therefore, pediatricians should not underestimate speech delay. On the other hand, due to the fact that, the regulations to receive special education and therapy often create anxiety for the parents, we think that this system needs to be updated and special education support should be determined by the special needs of each child.
Male and Female Partner-Caregivers' Burden: Does It Get Worse Over Time?

Background and Objectives The general view is that partner-caregiver burden increases over time but findings are inconsistent. Moreover, the pathways underlying caregiver burden may differ between men and women. This study examines to what degree and why partner-caregiver burden changes over time. It adopts Pearlin's Caregiver Stress Process Model, as it is expected that higher primary and secondary stressors will increase burden and larger amounts of resources will lower burden. Yet, the impact of stressors and resources may change over time. The wear-and-tear model predicts an increase of burden due to a stronger impact of stressors and lower impact of resources over time. Alternatively, the adaptation model predicts a decrease of burden due to a lower impact of stressors and higher impact of resources over time. Research Design and Methods We used 2 observations with a 1-year interval of 279 male and 443 female partner-caregivers, derived from the Netherlands Older Persons and Informal Caregivers Survey Minimum Data Set. We applied multilevel regression analysis, stratified by gender. Results Adjusted for all predictors, caregiver burden increased over time for both men and women. For female caregivers, the impact of poor spousal health on burden increased and the impact of fulfillment decreased over time. Among male caregivers, the impact of predictors did not change over time. Discussion and Implications The increase of burden over time supports the wear-and-tear model, in particular for women. This study highlights the need for gender-specific interventions that are focused on enabling older partners to be better prepared for long-term partner-care.
Mutations in the MAB_2299c TetR Regulator Confer Cross-Resistance to Clofazimine and Bedaquiline in Mycobacterium abscessus

New therapeutic approaches are needed against Mycobacterium abscessus, a respiratory mycobacterial pathogen that evades efforts to successfully treat infected patients. Clofazimine and bedaquiline, two drugs used for the treatment of multidrug-resistant tuberculosis, are being considered as alternatives for the treatment of lung diseases caused by M. abscessus. ABSTRACT New therapeutic approaches are needed against Mycobacterium abscessus, a respiratory mycobacterial pathogen that evades efforts to successfully treat infected patients. Clofazimine and bedaquiline, two drugs used for the treatment of multidrug-resistant tuberculosis, are being considered as alternatives for the treatment of lung diseases caused by M. abscessus. With the aim to understand the mechanism of action of these agents in M. abscessus, we sought herein to determine the means by which M. abscessus can develop resistance. Spontaneous resistant strains selected on clofazimine, followed by whole-genome sequencing, identified mutations in MAB_2299c, encoding a putative TetR transcriptional regulator. Unexpectedly, mutants with these mutations were also cross-resistant to bedaquiline. MAB_2299c was found to bind to its target DNA, located upstream of the divergently oriented MAB_2300-MAB_2301 gene cluster, encoding MmpS/MmpL membrane proteins. Point mutations or deletion of MAB_2299c was associated with the concomitant upregulation of the mmpS and mmpL transcripts and accounted for this cross-resistance. Strikingly, deletion of MAB_2300 and MAB_2301 in the MAB_2299c mutant strain restored susceptibility to bedaquiline and clofazimine. Overall, these results expand our knowledge with respect to the regulatory mechanisms of the MmpL family of proteins and a novel mechanism of drug resistance in this difficult-to-treat respiratory mycobacterial pathogen. Therefore, MAB_2299c may represent an important marker of resistance to be considered in the treatment of M. abscessus diseases with clofazimine and bedaquiline in clinical settings.
Method-Dependent Epidemiological Cutoff Values for Detection of Triazole Resistance in Candida and Aspergillus Species for the Sensititre YeastOne Colorimetric Broth and Etest Agar Diffusion Methods

Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex, 157 C. guilliermondii (Meyerozyma guilliermondii), 676 C. krusei (Pichia kudriavzevii), 298 C. lusitaniae (Clavispora lusitaniae), 911 C. parapsilosis sensu stricto, 3,691 C. parapsilosis species complex, 36 C. metapsilosis, 110 C. orthopsilosis, 1,854 C. tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A. flavus, 130 A. nidulans, 233 A. niger, and 302 A. terreus complex isolates. ABSTRACT Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex, 157 C. guilliermondii (Meyerozyma guilliermondii), 676 C. krusei (Pichia kudriavzevii), 298 C. lusitaniae (Clavispora lusitaniae), 911 C. parapsilosis sensu stricto, 3,691 C. parapsilosis species complex, 36 C. metapsilosis, 110 C. orthopsilosis, 1,854 C. tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A. flavus, 130 A. nidulans, 233 A. niger, and 302 A. terreus complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included: ERG11 (C. albicans), ERG11 and MRR1 (C. parapsilosis), cyp51A (A. fumigatus), and CDR2 and CDR1 overexpression (C. albicans and C. glabrata, respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for S. cerevisiae and 9 yeast and 3 Aspergillus species and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 µg/ml for C. albicans and the Etest itraconazole ECV of 2 µg/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of Aspergillus spp. Further evaluations should be conducted with more Candida mutants.
Inter-relationships among physical dimensions, distal–proximal rank orders, and basal GCaMP fluorescence levels in Ca2+ imaging of functionally distinct synaptic boutons at Drosophila neuromuscular junctions

Abstract GCaMP imaging is widely employed for investigating neuronal Ca2+ dynamics. The Drosophila larval neuromuscular junction (NMJ) consists of three distinct types of motor terminals (type Ib, Is and II). We investigated whether variability in synaptic bouton sizes and GCaMP expression levels confound interpretations of GCaMP readouts, in inferring the intrinsic Ca2+ handling properties among these functionally distinct synapses. Analysis of large data sets accumulated over years established the wide ranges of bouton sizes and GCaMP baseline fluorescence, with large overlaps among synaptic categories. We showed that bouton size and GCaMP baseline fluorescence were not confounding factors in determining the characteristic frequency responses among type Ib, Is and II synapses. More importantly, the drastic phenotypes that hyperexcitability mutations manifest preferentially in particular synaptic categories, were not obscured by bouton heterogeneity in physical size and GCaMP expression level. Our data enabled an extensive analysis of the distal–proximal gradient of GCaMP responses upon genetic and pharmacological manipulations. The results illustrate the conditions that disrupt or enhance the distal–proximal gradients. For example, stimulus frequencies just above the threshold level produced the steepest gradient in low Ca2+ (0.1 mM) saline, while supra-threshold stimulation flattened the gradient. Moreover, membrane hyperexcitability mutations (eag1 Sh120 and parabss1) and mitochondrial inhibition by dinitrophenol (DNP) disrupted the gradient. However, a novel distal–proximal gradient of decay kinetics appeared after long-term DNP incubation. We performed focal recording to assess the failure rates in transmission at low Ca2+ levels, which yielded indications of a mild distal–proximal gradient in release probability.
Reflections on self-determination theory as an organizing framework for personality psychology: Interfaces, integrations, issues, and unfinished business.

OBJECTIVE This special issue focuses on self-determination theory (SDT) as an integrative framework for the wider field of personality research. In this commentary our aims include: reflecting on the utility and strengths of SDT as such a general framework and responding to the various contributions in this issue regarding their use of SDT as a guiding, complementary, or contrasting framework. METHODS AND RESULTS We describe how SDT has developed organically and conservatively from "within" based on the emerging patterns of evidence, as well through the ongoing challenges from other models and frameworks. We then discuss each of the various contributions to this special issue, addressing themes that include SDT's breadth of methods, and its relevance to topics such as narcissism, wisdom, individual differences, Big-Five traits, and the neuropsychology of motivation, among others. Across these discussions, we highlight fruitful avenues for research and cross-fertilization across the fields of personality, development, motivation, and neuroscience. At the same time, we counter some claims made about SDT, and forward certain cautions regarding the integration of SDT and other personality frameworks and models. CONCLUSIONS We conclude by revisiting the value of broad theory, and SDT in particular, for coordinating complex research findings concerning motivation, personality development and wellness across multiple levels of analysis and, perhaps more importantly, for pointing researchers to the right questions within today's prolific empiricism.
No-reference remote sensing image quality assessment based on the region of interest and structural similarity

Blur and noise are two common distortion factors which affect remote sensing image quality. And make it difficult to assess the remote sensing image quality. The Structure Similarity(SSIM) algorithm is simple, high efficient and accurate. However, it does not work well when there is cross distortion in the image. To deal with the problem of SSIM algorithm treating different regions of image identically, this paper considered the perceptual characteristics to different content and masking effect. The proposed method is the perceptual weighting used in the region of interest and based on SSIM algorithm. The experiment shows that, compared with the Peak Signal-Noise Rate(PSNR) index, the proposed index has good consistence with the Structure Similarity(SSIM) index, and can make an effective and correct evaluation of image with both noise and blur. This is an accurate and reliable no-reference remote sensing image quality assessment mothed, which is easy to implement.