Miracle-dz/UniFAR-FTUs huggingface dataset:
Primary ductal adenocarcinoma of the lacrimal gland with changing genetic analysis mutations

ABSTRACT Primary ductal adenocarcinoma of the lacrimal gland is a rare but highly aggressive epithelial malignancy with a poor prognosis. Early diagnosis, along with genetic testing of these tumors, is imperative for proper management. We present a case of a 54-year-old man with decreasing vision over the past three years and increasing proptosis in his right eye over the past three months, secondary to a lacrimal gland mass diagnosed as primary ductal adenocarcinoma. The diagnosis was made using histological and immunohistochemical profiles (positivity for cytokeratin AE1/3, CAM5.2, androgen receptor, human epidermal growth factor receptor 2, and gross cystic disease fluid protein 15) seen in previous cases, alongside a tumor genetic profile that showed actionable mutations. Uniquely in this case, after failing traditional chemotherapy, repeat biopsy revealed a change in genetics with the malignancy no longer showing actionable mutations. These findings show that these immunohistochemical findings can act as diagnostic biomarkers, while genetic testing can reveal actionable mutations for targeted therapy.
Adolescent changes in implicit cognitions and prevention of substance abuse

This chapter explores the growth of implicit substance-use associations during early adolescence and their implications for the development of primary and secondary prevention programs. Increases in implicit measures of substance-use cognitions are highly correlated with, and prospectively predict, the initiation of youth alcohol and marijuana use. We propose that implicit memory measures may uniquely reveal decision-relevant indices of incentive motivation and behavioral choice. As such, implicit measures should be useful to (1) identify and target the stage of risk for substance-use initiatio n, (2) provide early sensitive measures of effective change from prevention programs, and (3) guide the development of new methods of persuasive messages that focus on behaviorally relevant cognitive changes.
Therapeutic Applications of Melatonin and Related Compounds

Increasing knowledge of the pharmacological effects of melatonin has suggested various possible therapeutic applications for the hormone. Because, as a natural substance, melatonin cannot be patented, melatonin-related compounds have been synthesized by industrial groups. The scope of such compounds is also to specifically target the recently discovered melatonin receptor subtypes. The sleep-inducing properties of melatonin are disputed, but are distinct from those of benzodiazepines. The observed effects on sleep latency or sleep efficiency, which remain to be confirmed, could be accounted for by the effects of melatonin on core body temperature and on circadian rhythms. There is also an urgent need for safety data, both in animals and in humans, particularly when long-term use is envisaged.
Salvaged allogeneic hematopoietic stem cell transplantation for pediatric chemotherapy refractory acute leukemia

There is an ongoing debate concerning the performance of salvaged allogeneic hematopoietic stem cell transplantation (allo-HSCT) in pediatric patients with acute refractory leukemia, in whom the prognosis is quite dismal. Few studies have ever been conducted on this subject. This may be partly due to missed opportunities by majority of the patients in such situations. To investigate the feasibility, evaluate the efficiency, and identify the prognostic factors of allo-HSCT in this sub-setting, the authors performed a single institution-based retrospective analysis. A total of 44 patients, of whom 28 had acute myeloid leukemia (AML), 13 had acute lymphocytic leukemia (ALL), and 3 had mixed phenotype leukemia (MPL), were enrolled in this study. With a median follow-up of 19 months, the estimated 2-year overall survival (OS) and progression free survival (PFS) were 34.3% (95% CI, 17.9–51.4%) and 33.6% (95% CI, 18.0–50.1%), respectively. The estimated 2-year incidence rates of relapse and non-relapse mortality (NRM) were 43.8% (95% CI 26.4–60.0%) and 19.6% (95% CI 9.1–32.9%), respectively. The estimated 100-day cumulative incidence of acute graft versus host disease (aGvHD) was 43.6% (95% CI 28.7–57.5%), and the 1-year cumulative incidence of chronic GvHD (cGvHD) was 45.5% (95% CI 30.5–59.3%). Compared with the previous studies, the multivariate analysis in this study additionally identified that female donors and cGvHD were associated with lower relapse and better PFS and OS. Male recipients, age younger than 10 years, a diagnosis of ALL, and the intermediate-adverse cytogenetic risk group were associated with increased relapse. On the contrary, extramedullary disease (EMD) and aGvHD were only linked to worse PFS. These data suggested that although only one-third of the patients would obtain PFS over 2 years, salvaged allo-HSCT is still the most reliable and best therapeutic strategy for refractory pediatric acute leukemia. If probable, choosing a female donor, better management of aGvHD, and induction of cGvHD promotes patient survival.
Optimum Placement of UAV as Relays

Unmanned aerial vehicles (UAVs) as aerial base stations or relays are becoming increasingly important in communications. In this letter, the optimum placement of a relaying UAV for maximum reliability is studied. The total power loss, the overall outage, and the overall bit error rate are derived as reliability measures. The optimum altitude is investigated for both static and mobile UAVs. Numerical results show that different reliability measures have slightly different optimum altitudes and that decode-and-forward is better than amplify-and-forward.
Heart rate biorhythm changes during the first three months of life.

UNLABELLED Heart rate (HR) was recorded in healthy full-term newborns aged 1-90 days. The aim of this study was to study the existence of circadian and/or ultradian rhythms in HR to determine maturity. HR was recorded during 24 h, at 30-min intervals, at different postnatal ages. Six-groups were investigated: day 1 (group 1); day 7 (group 7); day 15 (group 15); day 30 (group 30); day 60 (group 60), and day 90 (group 90). The chronograms for HR showed peaks and nadirs along the 24-hour periods, and the cosinor analysis proved the existence of 3-hour ultradian rhythm in groups 1, 7 and 30, and a 12-hour ultradian rhythm in group 90 (p < 0.01 in all cases). The same type of analysis confirmed the existence of a circadian rhythm in group 30. Similar results were obtained for groups 60 and 90 (p < 0.05). IN CONCLUSION at birth, newborns have an endogenous ultradian period of 3 h. A circadian rhythm appears within 15-30 days of postnatal life.
Viewing Sexual Desire as Stable Versus Fluid: The Impact of Implicit Beliefs on Women's Coping With Sexual Desire Problems

ABSTRACT Our goal was to examine how implicit theories can be applied to understanding women's coping responses to sexual problems. The belief that sexual desire changes over time is a type of incremental theory, while the belief that sexual desire is stable is a type of entity theory (Dweck, 2012). We examined how different implicit theories of sexual desire influence how women cope with sexual desire challenges. Also, we sought to determine whether women's perceptions of experiencing a sexual desire challenge moderate the relationship between their implicit beliefs and their coping responses. We randomly assigned women to read an article designed to induce either an entity or incremental theory about sexual desire. We further asked them to indicate how true it is that they have experienced, or are likely to experience, a sexual desire problem (1 = not at all true to 4 = very true). They then completed a measure of coping with desire problems, the Modified Cope Inventory. Results from both studies showed that women primed with entity theories who expected to experience a sexual desire issue reported significantly more negative coping than women primed with incremental theories. Implications of this research and future directions are discussed.
Imaginary hand movement classification using electroencephalography

This paper proposes a method to help patients who cannot control their appendicular organs to communicate and to control devices via a binary decision by electroencephalography (EEG). We exploited 12 volunteers' EEG datasets from PhysioNet (EEG Motor Movement/Imaginary Datasets) that contain imaginary hand movement. For the signal selection, we have selected theta and alpha bands (4–15 Hz), since the signals in these bands are distinctively changed by the imagination. For the method, we have applied power spectrum density estimated by the autoregressive model (AR-model) to extract features, and then used principal component analysis (PCA) in order to reduce those features before the classification step. To measure the quality of the derived features, we used a set of classifiers including the decision tree, K-nearest neighborhood, and ensemble classifier. For the experiment, we conducted both intra-user and inter-user approaches. The leave-one-out cross validation was applied in the intra-user experiment while the five-fold cross validation was applied in the inter-user experiment. The results show that the highest average of classification accuracy is achieved by the cubic K-NN (97.08%) in the inter-user experiment and by the weighted K-NN (91.88%) in intra-user experiment.
Prognostic Value of DNA Ploidy Patterns of Colorectal Adenocarcinoma: Univariate and Multivariate Analysis

Background/Aim: The prognostic significance of DNA ploidy patterns of colorectal cancer has not yet been settled. The present study was designed to determine the prognostic value of DNA ploidy patterns for colorectal adenocarcinomas after curative resection. Methods: DNA ploidy patterns of 140 colorectal adenocarcinomas were determined by DNA flow cytometry, and the prognostic significance of DNA ploidy patterns was evaluated by univariate as well as multivariate analysis. Results: DNA ploidy patterns were diploid in 75 (53.6%) and aneuploid in 65 patients (46.4%). DNA ploidy patterns did not correlate with any of conventional prognostic variables. Univariate analysis disclosed that Dukes B2, C1, and C2 stages of the disease (p < 0.01), positive nodal metastases (p < 0.01), invasion through the intestinal wall (p < 0.01), and poor tumor differentiation (p < 0.05) were associated with worsened survival, but no correlation was found between DNA patterns and survival of patients. Multivariate analysis disclosed that tumor penetration through the bowel wall was associated with poorer survival of patients but the DNA ploidy pattern had no prognostic significance. Conclusions: A significant prognostic variable for patients after curative resection of colorectal adenocarcinoma was penetration of tumor through the bowel wall but not DNA ploidy patterns.
Treatment-associated TP53 DNA-binding domain missense mutations in the pathogenesis of secondary gliosarcoma

Background Gliosarcoma is a rare variant of glioblastoma (GBM) that exhibits frequent mutations in TP53 and can develop in a secondary fashion after chemoradiation of a primary GBM. Whether temozolomide (TMZ)-induced mutagenesis of the TP53 DNA-binding domain (DBD) can drive the pathogenesis of gliosarcoma is unclear. Methods We identified a case of a primary GBM that rapidly progressed into secondary gliosarcoma shortly after chemoradiation was initiated. Bulk tumor was collected and gliomasphere cultures derived from both the pre- and post-treatment tumors. We performed targeted DNA sequencing and transcriptome analyses of the specimens to understand their phylogenetic relationship and identify differentially expressed gene pathways. Gliomaspheres from the primary GBM were treated with TMZ and then analyzed to compare patterns of mutagenesis in vivo and ex vivo. Results The pre- and post-treatment tumors shared EGFR, CDKN2A, and PTEN mutations, but only the secondary gliosarcoma exhibited TP53 DBD missense mutations. Two mutations, R110C, and R175H, were identified, each in distinct clones. Both were base transitions characteristic of TMZ mutagenesis. Gene expression analysis identified increased JAK-STAT signaling in the gliosarcoma, together with reduced expression of microRNAs known to regulate epithelial-mesenchymal transition. Ex vivo treatment of the GBM spheres with TMZ generated numerous variants in cancer driver genes, including TP53 and CDH1, which were mutated in the post-treatment tumor. Conclusions TMZ-induced TP53 gain-of-function mutations can have a driving role in secondary gliosarcoma pathogenesis. Analysis of variants identified in ex vivo TMZ-treated gliomaspheres may have utility in predicting GBM evolutionary trajectories in vivo during standard chemoradiation.
The essential role of phospho‐T38 CPI‐17 in the maintenance of physiological blood pressure using genetically modified mice

PKC‐potentiated phosphorylation‐dependent inhibitory protein of protein phosphatase 1 (CPI‐17), an endogenous myosin phosphatase inhibitory protein, is considered a key molecule for Ca2+ sensitization of the contractile apparatus. Here, we have used clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR‐associated protein 9 to generate CPI‐17‐deficient [knockout (KO)] and threonine 38 (T38)‐phosphoresistant mice [threonine mutant into alanine (TA)], and then effects of CPI‐17 on vascular contractility in vitro and mean blood pressure (MBP) in vivo were investigated. In isolated thoracic aorta, phorbol 12,13‐dibutyrate induced a sustained contraction of wild‐type (WT) mice, whereas no contraction showed from TA or KO mice. A high concentration of KCl solution‐induced contraction was not different between transgenic and WT mice. In contrast, phenylephrine (PE)‐induced contractions in both mutant strains were significantly smaller than those of WT mice in association with a low level of myosin phosphorylation, suggesting that at least part of PE‐induced contraction is regulated by phosphorylation of CPI‐17 at T38. Finally, the physiologic role of CPI‐17 in the regulation of blood pressure was investigated using radio telemetry. MBP was decreased significantly in both transgenic mice, even with a compensatory increase in heart rate. In summary, we generated KO and constitutively phospho‐resistant mouse models of CPI‐17 for the first time. p‐CPI‐17 at T38, possibly by PKC, could be important to maintain vascular contractility and blood pressure in vivo. — Yang, Q. , Fujii, W. , Kaji, N. , Kakuta, S. , Kada, K. , Kuwahara, M. , Tsubone, H. , Ozaki, H. , Hori, M. The essential role of phospho‐T38 CPI‐17 in the maintenance of physiological blood pressure using genetically modified mice. FASEB J. 32, 2095–2109 (2018). www.fasebj. org
Experimental Study for Cultivating College Students’ Sports Motivation in Sport Education Model

The paper analyzes the sport motivation change of students before and after sport education model by setting and implementing sport education model, which takes volleyball elective course in university for example. Sport education volleyball season model is adopted in this paper. Questionnaires were sent to respondents in experimental class before and after the experiment. Finally, statistical analysis was performed after qualified questionnaires were collected. After sport education model experiment, students’ self-determination index was significantly higher than before (p p > 0.05). Test indicated that freshman’ external regulation motivation was more obviously than sophomore (p p < 0.05). Sport education volleyball season model enhances students’ intrinsic motivation of sports, and meets students’ psychological need to inspire their participation in physical education. Sport education model is applicable to all students. Thus, the result helps to popularize the sport education model.
Lactate Transport by Cortical Synaptosomes from Adult Rat Brain: Characterization of Kinetics and Inhibitor Specificity

Since lactate released by glial cells may be a key substrate for energy in neurons, the kinetics for the uptake of L-[U-14C]lactate by cortical synaptic terminals from 7- to 8-week-old rat brain were determined. Lactate uptake was temperature-dependent, and increased by 64.9% at pH 6.2, and decreased by 43.4% at pH 8.2 relative to uptake at pH 7.3. Uptake of monocarboxylic acids was saturable with increasing substrate concentration. Eadie-Hofstee plots of the data gave evidence of two carrier-mediated uptake mechanisms with a high-affinity Km of 0.66 mM and Vmax of 3.66 mM for pyruvate, and a low-affinity system with a Km of 9.9 mM for both lactate and pyruvate and Vmax values of 16.6 and 23.1 nmol/30 s/mg protein for lactate and pyruvate, respectively. Saturable uptake was seen in the presence of 10 mM α-cyano-4-hydroxycinnamate. Lactate transport by synaptic terminals was much more sensitive to inhibition by sulfhydryl reagents than transport in astrocytes. Addition of 0.5 and 2 mM mersalyl decreased the uptake of 1 mM lactate by synaptic terminals by 59.3 and 66.37%, respectively. Pyruvate moderately decreased lactate transport, whereas 3-hydroxybutyrate had little effect. Quercetin, an inhibitor of lactate release, had little effect on the content of 14C lactate in synaptic terminals, supporting the concept that the majority of lactate produced within brain is from glial cells. Oxidation of L-[U-14C]lactate by synaptosomes was saturable, and yielded a Km of 1.23 mM and a Vmax of 116 nmol/h/mg protein. Overall the studies show that synaptic terminals from adult brain have a high capacity for transport and oxidation of lactate, consistent with the proposed role for this compound in metabolic trafficking in brain. Furthermore, the data provide kinetic evidence of two carrier-mediated mechanisms for monocarboxylic acid transport by synaptosomes and demonstrate that uptake of lactate by synaptic terminals is regulated differently than transport by astrocytes. Uptake of lactate by synaptic terminals also has differences from the systems described for neurons.
Analysis of Chromosome 3, 7 and 8 Centromeric Regions in Bronchial Lavage Specimens by FISH.

OBJECTIVES Multiple genetic changes are observed in malignant tumors but are rare or absent in benign conditions. Aneuploidy is the most common feature of solid tumors including lung cancer and diagnosis of malignant tumors is possible through detection of aneuploidy. The aim of this study was to investigate chromosomal abnormalities in cells from non-small cell lung cancer patients obtained bronchoscopically and to evaluate the suitability of fluorescence in situ hybridization (FISH). MATERIAL AND METHODS Bronchial lavage samples of 17 non-small cell lung cancer (NSCLC) patients were evaluated with four-color FISH using deoxyribonucleic acid (DNA) probes specific for the centromere regions of chromosomes 3, 7 and 8. tested specimens were first hybridized with probes, then visualized under fluorescence microscobe and captured with device's camera. RESULTS High number of aneuploidic cells were detected in all the samples. Increased or decreased abnormal copies or chromosomes 3, 7 and 8 were obserced in all the 17 patients. Aneuploidy of chromosome 3 (21.35%) was higher than those of chromosome 7 (9.06%) and chromosome 8 (15.47%). Moreover, our results were significant for monosomy and trisomy of chromosome 3, trisomy of chromosome 7, nullisomy, monosomy and trisomy of, chromosome 8 (p< 0.05). CONCLUSION It has been observed that FISH is a useful technique for detection of aneuploidy in bronchial lavage samples obtained by bronchoscopy. Interphase cells were evaluated without cell culturing with this method and high number of tumor cells were enumerated rapidly. Our study has demonstrated that, FISH technique may be used successfully in detection of chromosome number abnormalities in NSCLC patients and may facilitate evaluation of genetic abnormalities.
Investigating the potential impacts of climate change on a marine turtle population

Recent increases in global temperatures have affected the phenology and survival of many species of plants and animals. We investigated a case study of the effects of potential climate change on a thermally sensitive species, the loggerhead sea turtle, at a breeding location at the northerly extent of the range of regular nesting in the United States. In addition to the physical limits imposed by temperature on this ectothermic species, sea turtle primary sex ratio is determined by the temperature experienced by eggs during the middle third of incubation. We recorded sand temperatures and used historical air temperatures (ATs) at Bald Head Island, NC, to examine past and predict future sex ratios under scenarios of warming. There were no significant temporal trends in primary sex ratio evident in recent years and estimated mean annual sex ratio was 58% female. Similarly, there were no temporal trends in phenology but earlier nesting and longer nesting seasons were correlated with warmer sea surface temperature. We modelled the effects of incremental increases in mean AT of up to 7.5°C, the maximum predicted increase under modelled scenarios, which would lead to 100% female hatchling production and lethally high incubation temperatures, causing reduction in hatchling production. Populations of turtles in more southern parts of the United States are currently highly female biased and are likely to become ultra‐biased with as little as 1°C of warming and experience extreme levels of mortality if warming exceeds 3°C. The lack of a demonstrable increase in AT in North Carolina in recent decades coupled with primary sex ratios that are not highly biased means that the male offspring from North Carolina could play an increasingly important role in the future viability of the loggerhead turtle in the Western Atlantic.
The effect of different seeding densities of linseed (Linum usitatissimum L.) on flax flea beetles (Coleoptera: Chrysomelidae)

Linseed, one of the oldest cultivated crops, is again gaining in importance, mainly due to its nutritional benefi ts and biomedical applications. Th erefore, it is expected that herbivores will also exist in greater abundance. Among them the fl ea beetle, Aphthona euphorbiae Schrank and Longitarsus parvulus Paykull are considered to be serious pests of fl ax grown for fi bre and seeds in Europe. Th e aim of this study was to determine fl ax fl ea beetles’ abundance, species richness and seasonal dynamics on linseed grown at diff erent densities. It was expected that linseed seeding density can signifi cantly aff ect fl ea beetle populations. Th e experiment was carried out in Lower Silesia, Poland, from 2011 to 2013. A genetically modifi ed type of linseed overproducing fl avonoids was used. Flea beetles and the damages they caused were determined on plants and also a sweep net was used for the collection of adult beetles. During the three years of the study 15 species of fl ea beetles were identifi ed from oil fl ax plants, with A. euphorbiae and L. parvulus being dominant. In terms of the total catch, the tendency was for beetle numbers to decrease with increasing plant density. Flax fl ea beetles feeding on linseed plants, irrespective of plant density, had two peaks of abundance. Th e fi rst peak was lower and occurred in June, when plants were at the blooming stage. Th is peak was caused by overwintering adults who colonized crops in spring. Th e second, higher peak of abundance was recorded in the second half of July, when plants were at the ripening stage. Th is peak was formed by adults of the new generation. Each year, at the higher population peak of abundance, the fl ea beetles were most numerous on plants grown at the lowest density. Th ere was one period, lasting either from mid-May to the fi rst few days of June, or from the beginning of June to mid-June, during which the number of holes and damage on plants of each treatment were highest. During the three years of the study there were several cases of signifi cantly higher numbers of fl ea beetle feeding symptoms on plants grown at the lowest density as compared to the medium and highest densities.