Miracle-dz/UniFAR-FTUs huggingface dataset:
Cell Lines Derived From Feline Fibrosarcoma Display Unstable Chromosomal Aneuploidy and Additionally Centrosome Number Aberrations

The purpose of the study was to evaluate clonality and presence of numerical chromosomal and centrosomal aberrations in 5 established feline fibrosarcoma cell lines and in a fetal dermal fibroblast cell line as a control. The clonality of all cell lines was examined using limited-dilution cloning. The number of chromosomes was counted in metaphase spreads. The immunocytochemical analysis of centrosome numbers was performed by indirect immunofluorescence using a monoclonal antibody that targets γ-tubulin, a well-characterized component of centrosomes. Monoclonal cell populations could be established from all cell lines. In all feline fibrosarcoma cell lines, the number of chromosomes deviated abnormally from the normal feline chromosome number of 2n = 38, ranging from 19 to 155 chromosomes per cell. Centrosome hyperamplification was observed in all 5 feline fibrosarcoma cell lines with a proportion of cells (5.7 to 15.2%) having more than 2 centrosomes. In the control cell line, only 0.6% of the cells had more than 2 centrosomes. In conclusion, the examinations revealed that centrosome hyperamplification occurs in feline fibrosarcoma cell lines. The feline fibrosarcoma cell lines possessed 10 to 25 times as many cells with centrosome hyperamplification as the control cell line. These observations suggest an association of numerical centrosome aberrations with karyotype instability by increasing the frequency of chromosome missegregation. The results of this study may be helpful for further characterization of feline fibrosarcomas and may contribute to the knowledge of cytogenetic factors that may be important for the pathogenesis of feline fibrosarcomas.
Neoplastic and Nonneoplastic Liver Lesions Induced by Dimethylnitrosamine in Japanese Medaka Fish

Small fish models have been used for decades in carcinogenicity testing. Demonstration of common morphological changes associated with specific mechanisms is a clear avenue by which data can be compared across divergent phyletic levels. Dimethylnitrosamine, used in rats to model human alcoholic cirrhosis and hepatic neoplasia, is also a potent hepatotoxin and carcinogen in fish. We recently reported some striking differences in the mutagenicity of DMN in lambda cII transgenic medaka fish vs. Big Blue® rats, but the pre-neoplastic and neoplastic commonalities between the two models are largely unknown. Here, we focus on these commonalities, with special emphasis on the TGF-β pathway and its corresponding role in DMN-induced hepatic neoplasia. Similar to mammals, hepatocellular necrosis, regeneration, and dysplasia; hepatic stellate cell and “spindle cell” proliferation; hepatocellular and biliary carcinomas; and TGF-β1 expression by dysplastic hepatocytes all occurred in DMN-exposed medaka. Positive TGF-β1 staining increased with increasing DMN exposure in bile preductular epithelial cells, intermediate cells, immature hepatocytes and fewer mature hepatocytes. Muscle specific actin identified hepatic stellate cells in DMN-exposed fish. Additional mechanistic comparisons between animal models at different phyletic levels will continue to facilitate the interspecies extrapolations that are so critical to toxicological risk assessments.
Spontaneous Pulmonary Alveolar Proteinosis in Captive “Moustached Tamarins” (Saguinus mystax)

Pulmonary alveolar proteinosis is a rare human disease characterized by accumulation of surfactant in alveoli without generating an inflammatory response. Lung lesions resembling pulmonary alveolar proteinosis were observed in 7 adult tamarins (5 males and 2 females). Gross lesions were characterized by areas of discoloration, slight bulging over the lung parenchyma, and occasional consolidation. Histologic examination of tamarin lung samples revealed intra-alveolar accumulation of amorphous, amphophilic, periodic acid–Schiff-positive, finely granular to dense material. In some cases, type II pneumocyte hypertrophy and hyperplasia were observed with pleural and septal thickening and fibrosis. Large numbers of intra-alveolar foamy macrophages were noted surrounding and/or in the vicinity of the lesions. Immunohistochemical analysis of the lung lesions using polyclonal (surfactant proteins A, B, and C) and monoclonal (surfactant protein D) antibodies revealed the granular material to be composed largely of surfactant protein B, followed by surfactant protein A. Surfactant proteins C and D were present in lesser quantities, with the latter observed surrounding the lipoproteinaceous deposits. Transmission electron microscopy of the affected lungs showed numerous, irregularly shaped osmiophilic lamellar bodies in type II pneumocytes. The cytoplasm in alveolar macrophages was expanded, containing ingested surfactant with swollen mitochondria and rough endoplasmic reticulum. Thoracic radiographs, available in 1 animal, depicted the lesions as small multifocal opacities randomly distributed in cranial and diaphragmatic lung lobes. This is, to the authors’ knowledge, the first report of spontaneous pulmonary alveolar proteinosis in nonhuman primates.
Initiating and Cancer-Propagating Cells in TEL-AML1-Associated Childhood Leukemia

Understanding cancer pathogenesis requires knowledge of not only the specific contributory genetic mutations but also the cellular framework in which they arise and function. Here we explore the clonal evolution of a form of childhood precursor–B cell acute lymphoblastic leukemia that is characterized by a chromosomal translocation generating a TEL-AML1 fusion gene. We identify a cell compartment in leukemic children that can propagate leukemia when transplanted in mice. By studying a monochorionic twin pair, one preleukemic and one with frank leukemia, we establish the lineal relationship between these “cancer-propagating” cells and the preleukemic cell in which the TEL-AML1 fusion first arises or has functional impact. Analysis of TEL-AML1–transduced cord blood cells suggests that TEL-AML1 functions as a first-hit mutation by endowing this preleukemic cell with altered self-renewal and survival properties.
Alcoolização de nódulo tiroidiano em região endêmica de bócio colóide

Thyroid nodular disease is a common clinical disorder mainly in iodine lacking regions. This study has evaluated the effectiveness of percutaneous ethanol injection (PEI) in the treatment of single, multiple, solid, and cystic thyroid nodules of different sizes with or without glandular dysfunction, in goiter endemic region. Forty-two patients with diagnosis of colloid goiter or colloid nodular hyperplasia in fine-needle aspiration (FNA) were selected for analysis, after having been submitted to at least two PEI. Thyroid nodules were multiple (solid and or cystic) in 52.4% of the patients, single and solid or mixed in 35.7%, and single and cystic in 11.9%. The mean reduction of nodules after ethanol injection was of 58.2% in the single and of 60.8% in the cystic ones. The reduction of the whole thyroid lobe was evaluated in the multiple nodules and it reached 52.4%. The side-effects were registered only as a consequence of the application discomfort. This study points out that the percutaneous ethanol injection reaching volume mean reductions, varying from 49% to 60%, is a safe, effective, and simple method for the treatment of benign thyroid nodules.
Predicting Human Interactive Learning by Regret-Driven Neural Networks

Much of human learning in a social context has an interactive nature: What an individual learns is affected by what other individuals are learning at the same time. Games represent a widely accepted paradigm for representing interactive decision-making. We explored the potential value of neural networks for modeling and predicting human interactive learning in repeated games. We found that even very simple learning networks, driven by regret-based feedback, accurately predict observed human behavior in different experiments on 21 games with unique equilibria in mixed strategies. Introducing regret in the feedback dramatically improved the performance of the neural network. We show that regret-based models provide better predictions of learning than established economic models.
Natural Heterobilharzia americana Infection in Horses in Texas

The schistosome Heterobilharzia americana infects dogs, raccoons, and other mammals in the southeastern United States. Migration of eggs into the liver results in parasitic granulomas with varying degrees of fibrosis and inflammation. Recently, hepatic parasitic granulomas in horses were shown to be caused by H. americana infection. In the present study, samples of liver from 11 of 12 horses with hepatic granulomas identified at necropsy (n = 11) or surgical biopsy (n = 1) were used for DNA extraction, polymerase chain reaction amplification and sequencing using primers specific for a portion of the H. americana small subunit ribosomal RNA gene. A polymerase chain reaction amplicon of the correct size was produced from the extracted DNA in 8 of the 11 horses. Amplicons from 5 of the 8 positive horses were sequenced and had 100% identity with H. americana. In all but 2 of the 12 horses, Heterobilharzia was not responsible for the primary clinical disease, and the hepatic granulomas were considered an incidental finding.
Irreconcilable Differences: Fine-Root Life Spans and Soil Carbon Persistence

The residence time of fine-root carbon in soil is one of the least understood aspects of the global carbon cycle, and fine-root dynamics are one of the least understood aspects of plant function. Most recent studies of these belowground dynamics have used one of two methodological strategies. In one approach, based on analysis of carbon isotopes, the persistence of carbon is inferred; in the other, based on direct observations of roots with cameras, the longevity of individual roots is measured. We show that the contribution of fine roots to the global carbon cycle has been overstated because observations of root lifetimes systematically overestimate the turnover of fine-root biomass. On the other hand, isotopic techniques systematically underestimate the turnover of individual roots. These differences, by virtue of the separate processes or pools measured, are irreconcilable.
Assessment of Knowledge, Attitude and Practices on Reproductive Health Among Ambo University Students in Ambo, Oromia National Regional State, Ethiopia, 2013/14

Introduction: Adolescent’s sexuality and Reproductive Health are generally not well addressed to protect them from unwanted pregnancies, complications of unsafe abortions and sexual transmitted infection including human immunodeficiency virus/AIDS. Although the numbers of young people who need information and services are enormous, services are scarce, fragmented and nonexistent. Objective: To assess the level of Knowledge, Attitudes and Practice towards reproductive health among students of Ambo university. Methods: Institutional based descriptive cross-sectional study design supplemented by qualitative in-depth interview was conducted among Ambo University students, Ambo, western Ethiopia from December to January 2013/14. Multistage sampling technique was implemented and sample size taken was 605. Data were collected using structured and pretested questionnaires and entered into EPI Info 3.5. 1 then exported to SPSS Version 16 for analysis. Result: Respondent were mentioned different methods to prevent reproductive health problem, and from these 111(18.5%) mentioned condom, 44 (7.9%) Abstain as the method of choice while 322(53.7%) mentioned that they didn't know prevention methods. From the total students participated in the study 487(81.2%) have shown interest to use reproductive health service in the future and 350(84.7%) of the respondents wanted the establishment of reproductive health services within the university campus. 108(42%) of the respondents were started sex before the age of 18 years and only 6(2.4%) of the students were start sex after 24 years. 179(73.5%) of the students were not using contraceptive during the survey period Conclusion: the study showed that most the respondent didn't know reproductive health components, problems and as well didn't mention prevention methods. A great number of students were not used reproductive health services during the survey period; but there will be high demand to utilize reproductive health services. Recommendation: Since there was high demand on reproductive health services; Policy Makers(Program planners) should establish friendly reproductive health services within the university to increase coverage and prevent the students from reproductive health related problems and complications.
Helicobacter pylori, Gastroduodenal Disease, and Recurrent Abdominal Pain in Children

Objective. —To assess the evidence for a cause-and-effect relationship between Helicobacter pylori infection and antral gastritis, peptic ulcer disease, and recurrent abdominal pain in children. Data Sources. —A MEDLINE search from January 1983 through July 1994 was used to identify pertinent English-language publications. Current Contents and selected specialty journals were searched manually. Editorials, reviews, case reports, abstracts, and letters to the editor were excluded. Study Selection. —All studies in children (ie, 0 to 18 years) were included. Data Extraction. —Hill's criteria for causal inference were used to determine the strength of the evidence for a causal relationship. Data Synthesis. —In total, 45 studies (case series, cross-sectional surveys, and treatment trials) were retrieved. The rate ratio of antral gastritis in children with H pylori infection (compared with uninfected children) ranged from 1.9 to 71.0 (median, 4.6). The prevalence of H pylori infection in children with duodenal ulcer was high (range, 33% to 100%; median, 92%) compared with children with gastric ulcer (range, 11% to 75%; median, 25%). Prevalence rates of infection in children with recurrent abdominal pain were inconsistent (range, 0% to 81%; median, 22%), with lower rates in children meeting Apley's criteria (range, 0% to 9%; median, 6%). Conclusions. —There is strong evidence for an association between H pylori infection and antral gastritis and duodenal ulcer disease in children; however, data from randomized, double-blind, placebo-controlled treatment trials are required. There is weak evidence for an association with gastric ulcer and weak or no evidence for an association with recurrent abdominal pain. ( JAMA . 1995;273:729-734)
Buffer overflow and format string overflow vulnerabilities

Buffer overflow vulnerabilities are among the most widespread of security problems. Numerous incidents of buffer overflow attacks have been reported and many solutions have been proposed, but a solution that is both complete and highly practical is yet to be found. Another kind of vulnerability called format string overflow has recently been found and although not as widespread as buffer overflow, format string overflow attacks are no less dangerous.
Salient Lesions in Domestic Ruminants Infected With the Emerging So-called Schmallenberg Virus in Germany

The so-called Schmallenberg virus (SBV), first detected in a German town of the same name in October 2011, is a novel emerging orthobunyavirus in Europe causing malformations and severe economic loss in ruminants. This report describes lesions in 40 sheep, 2 goats, and 16 cattle naturally infected with SBV as determined by real-time quantitative reverse transcription polymerase chain reaction. The most common macroscopic changes were arthrogryposis, vertebral malformations, brachygnathia inferior, and malformations of the central nervous system, including hydranencephaly, porencephaly, hydrocephalus, cerebellar hypoplasia, and micromyelia. Histologic lesions included lymphohistiocytic meningoencephalomyelitis in some cases, glial nodules mainly in the mesencephalon and hippocampus of lambs and goats, and neuronal degeneration and necrosis mainly in the brain stem of calves. Micromyelia was characterized by a loss of gray and white matter, with few neurons remaining in the ventral horn in calves. The skeletal muscles had myofibrillar hypoplasia in lambs and calves. The lesions of SBV-associated abortion and perinatal death are similar to those attributed to Akabane virus and other viruses in the Simbu group of bunyaviruses.
Pathology in Context

Orchestration of leukocyte diapedesis is complex, whether a direct transcellular migration or around the side, employing a paracellular approach at endothelial borders. Familiarity with the molecules and mechanisms involved and the temporal and spatial relationships is essential for more reasons than success on the general pathology portion of certification examination! Understanding this process is critical to the evolution of novel anti-inflammatory approaches. Disease conditions such as myocardial infarction, ulcerative colitis and Crohn disease are only a few examples that could benefit from minimizing leukocyte diapedesis. The review in this issue (‘‘Getting Leukocytes to the Site of Inflammation,’’ Vet Pathol, 2013;50:7–22) provides an essential update as to how leukocytes arrive at an inflamed area.
Detection of Porcine Circovirus Type 2 and Viral Replication by In Situ Hybridization in Primary Lymphoid Organs From Naturally and Experimentally Infected Pigs

Porcine circovirus type 2 (PCV2) infection is the cause of postweaning multisystemic wasting syndrome (PMWS). It has been speculated whether cell types permissive of replication are found in the primary lymphoid organs and whether infection of these tissues has an important role in the pathogenesis of PMWS. The aim of this study was to determine if primary lymphoid organ cells support viral replication during PCV2 infection. This was done by histopathological examination of thymus and bone marrow from pigs experimentally inoculated with PCV2 (n = 24), mock-infected pigs (n = 12), pigs naturally affected by PMWS (n = 33), and age-matched healthy control animals (n = 29). In situ hybridization (ISH) techniques were used to detect PCV2 nucleic acid irrespective of replicative status (complementary probe, CP) or to detect only the replicative form of the virus (replicative form probe, RFP). PCV2 was not detected in the experimentally PCV2-inoculated pigs or the control animals. Among the PMWS-affected pigs, 19 of 20 (95%) thymuses were positive for PCV2 by CP ISH, and 7 of 19 (37%) of these also supported viral replication. By CP ISH, PCV2 was detected in 16 of 33 (48%) bone marrow samples, and 5 of 16 (31%) of these also supported replication. The 2 ISH probes labeled the same cell types, which were histiocytes in both organs and lymphocytes in thymus. The RFP labeled fewer cells than the CP. Thus, PCV2 nucleic acids and replication were found in bone marrow and thymus of PMWS-affected pigs, but there was no evidence that primary lymphoid organ cells are major supporters of PCV2 replication.
Microbial Ecology of Ocean Biogeochemistry: A Community Perspective

The oceans harbor a tremendous diversity of marine microbes. Different functional groups of bacteria, archaea, and protists arise from this diversity to dominate various habitats and drive globally important biogeochemical cycles. Explanations for the distribution of microbial taxa and their associated activity often focus on resource availability and abiotic conditions. However, the continual reshaping of communities by mortality, allelopathy, symbiosis, and other processes shows that community interactions exert strong selective pressure on marine microbes. Deeper exploration of microbial interactions is now possible via molecular prospecting and taxon-specific experimental approaches. A holistic outlook that encompasses the full array of selective pressures on individuals will help elucidate the maintenance of microbial diversity and the regulation of biogeochemical reactions by planktonic communities.
Pathology and Diagnostic Criteria of Clostridium difficile Enteric Infection in Horses

Clostridium difficile is commonly associated with diarrhea and colitis in humans and other mammals, including horses. To this date, the epidemiologic, microbiologic, clinical, and diagnostic aspects of C. difficile–associated disease (CDAD) in horses have been thoroughly described. However, reports describing the enteric pathology of this disease in horses are limited. This study presents a comprehensive description of the pathologic characteristics of CDAD in 21 horses and discusses the criteria for the diagnosis of the disease. Case selection was based on C. difficile A/B toxins detection (enzyme-linked immunosorbent assay) in intestinal content samples accompanied by compatible gross and microscopic enteric lesions. Grossly, multifocal, segmental, or diffuse hemorrhage; congestion; and/or marked gelatinous edema of the intestinal wall with abundant bloody or green watery contents were observed. Histologically, the most common lesion was severe necrotizing or necrohemorrhagic enteritis, colitis, or typhlocolitis, with mucosal and/or submucosal thrombosis and marked submucosal edema. The pathology of CDAD in horses is similar to that caused by other equine enteric pathogens; therefore, a definitive diagnosis requires detection of C. difficile A/B toxins in the intestinal contents.
Effects of Replacing Toasted Sorrel Seed (Hibiscus Sabdariffa) Meal for Soybean Meal in Broiler Finisher Diet.

The study was undertaken to determine the growth performance and internal weight organ of broiler fed varying level of Toasted Sorrel Seed Meal (TSSM) Hibiscus sabdariffa for soybeans meal in a broiler finisher ration. Seventy Five 4 weeks old 4 weeks old Anak 2000 white strain birds were used in the study. The birds were divided into five treatments groups of fifteen birds in a Completely Randomized Design (CRD). The treatments had soybean meal quantitatively substituted at 0%, 5%, 15%, and 20% respectively by Toasted Sorrel Seed meal (TSSM). At 28 days of age, 3 broilers of comparable live weights per replicate were selected, starved for 12 hours, slaughtered and eviscerated to determine the dressed weight at increasing levels of TSSM in the diet. There was significant (P
A survey of consumer awareness of salt in food and reported purchasing activity towards low salt foods

A MARKET research study of 160 adult respondents of both sexes subdivided into under-25 and over-25- year-old age groups was undertaken in Nottingham between January and April 1992. The aims of the study were to determine whether consumers per ceived salt in food as a health problem, their knowl edge of the salt content of selected foods, and whether they reported changes in purchasing ac tivity towards low salt food products. A high pro portion of respondents in this survey (83.8 per cent) believed salt to be detrimental to health but overall there was a poor level of knowledge of the salt con tent of a range of foods. Eighty-five per cent of re spondents had purchased reduced salt food products and 64.6 per cent of these had become regular users. Product development of actively promoted reduced salt foods offers potential, provided palatability and food safety problems are overcome.