Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of norovirus infections in Seoul, Korea

The present study has determined the detection rate of norovirus (NoV) with acute gastroenteritis (AGE) in hospitalized children and describes the molecular epidemiology of NoV circulating in Seoul, Korea. Six hundred and eighty‐three (9.8%) of samples were positive for NoV. Of these, the NoV GII genogroup was the most commonly found, with a prevalence of 96.2% (683 of 710). Only 27 samples were positive for the NoV GI genogroup. Ten kinds of GI genotype (GI/1, GI/2, GI/3, GI/4, GI/5, GI/6, GI/7, GI/9, GI/12, and GI/13) and eight kinds of GII genotype (GII/2, GII/3, GII/4, GII/8, GII/14, GII/15, GII/16, and GII/17) were identified in children with AGE during the years 2008–2011.
GU METRIC - A New Feature Selection Algorithm for Text Categorization

A pipettor for threaded attachment to a liquid containing jar provides for dispensing a controllable volume of liquid with a high degree of precision upon manually operating a plunger. The liquid volume dispensed in a single plunger operation is adjusted by rotation of the plunger knob. Both a linear coarse scale and a circular fine adjustment scale are provided for accurately setting the volume to be dispensed. Positive detent stops are provided for accurate volume settings. Improved air purging and plunger locking mechanisms are also provided.
mRNA Decapping in Yeast Requires Dissociation of the Cap Binding Protein, Eukaryotic Translation Initiation Factor 4E

ABSTRACT A major pathway of eukaryotic mRNA turnover occurs by deadenylation-dependent decapping that exposes the transcript to 5′→3′ exonucleolytic degradation. A critical step in this pathway is decapping, since removal of the cap structure permits 5′→3′ exonucleolytic digestion. Based on alterations in mRNA decay rate from strains deficient in translation initiation, it has been proposed that the decapping rate is modulated by a competition between the cytoplasmic cap binding complex, which promotes translation initiation, and the decapping enzyme, Dcp1p. In order to test this model directly, we examined the functional interaction of Dcp1p and the cap binding protein, eukaryotic translation initiation factor 4E (eIF4E), in vitro. These experiments indicated that eIF4E is an inhibitor of Dcp1p in vitro due to its ability to bind the 5′ cap structure. In addition, we demonstrate that in vivo a temperature-sensitive allele of eIF4E (cdc33-42) suppressed the decapping defect of a partial loss-of-function allele of DCP1. These results argue that dissociation of eIF4E from the cap structure is required before decapping. Interestingly, the temperature-sensitive allele of eIF4E does not suppress the decapping defect seen in strains lacking the decapping activators, Lsm1p and Pat1p. This indicates that these activators of decapping affect a step in mRNA turnover distinct from the competition between Dcp1 and eIF4E.
Combined Therapy for Migraine Prevention? Clinical Experience with A β-Blocker Plus Sodium Valproate in 52 Resistant Migraine Patients

The aim was to explore whether combining a β-blocker and sodium valproate could lead to an advantage in efficacy in patients with migraine previously resistant to the two medications in monotherapy. Fifty-two patients (43 women) with a history of episodic migraine with or without aura, and previously unresponsive to β-blockers and sodium valproate in monotherapy, were treated with a combination of propranolol or nadolol and sodium valproate in an open-label fashion. Eight patients (15%) discontinued due to adverse events. Fifteen (29%) did not respond. The remaining 29 cases (56%) showed response (>50% reduction in migraine days). The response was excellent in nine (17%). From this open trial, combination therapy with a β-blocker and sodium valproate appears to be a good migraine preventative in some previously resistant migraine cases. Controlled trials are now necessary to determine the true advantage in efficacy of this combination in difficult to treat migraineurs.
Marked enhancement by rifampicin and lack of effect of isoniazid on the elimination of quinine in man.

The effect of rifampicin and isoniazid pretreatment on the pharmacokinetics of quinine after a single oral dose (600 mg quinine sulphate) was studied in nine healthy young Thai male volunteers using a three-way randomized crossover design. Subjects were studied over three 2 day periods, during which they received no pretreatment, or pretreatment with daily 600 mg p.o. rifampicin for 2 weeks, or isoniazid 300 mg p.o. daily for 1 week, prior to quinine administration. The mean (+/- s.d.) clearance (CL/F) of quinine coadministered with rifampicin (0.87 +/- 0.35 1 h-1 kg-1) was significantly greater than that of quinine alone (0.14 +/- 0.05 1 h-1 kg-1). The mean difference in clearance from the control treatment was 0.73 1 h-1 kg-1, with 95% confidence interval (C.I.) of 0.48 to 0.98. The unbound clearance (CLu/F) of quinine, which reflects the activity of the drug-metabolizing enzymes, was considerably greater (6.9-fold) in subjects when rifampicin was coadministered with quinine than that of quinine alone (6.9 +/- 3.6 vs 1.0 +/- 0.5 1 h-1 kg-1; the 95% C.I. for the mean difference was 3.3 to 8.5). The mean elimination half-life of quinine when coadministered with rifampicin (5.5 +/- 3.0 h) was significantly shorter than when quinine was given alone (11.1 +/- 3.0 h; the 95% C.I. for the mean difference was -8.6 to -2.6). In contrast to rifampicin, pretreatment for 1 week with 300 mg oral isoniazid had no significant effects on the pharmacokinetics of quinine. (ABSTRACT TRUNCATED AT 250 WORDS)
Morpho-Histological architecture of various cells with special emphasis on the seasonal variations of gonadotrophs in the pituitary gland of Notopterus notopterus (Pallas, 1769) in relation to testicular maturation

The pituitary gland of Notopterus notopterus (Pallas, 1769) was situated in a shallow pit at the floor of the skull and attached to the infundibulum. On the basis of tinctorial properties the cell types of pituitary were indentified which were distributed in the rostral pars distalis (RPD) proximal pars distalis (PPD) and pars intermedia (PI) in different proportions. The three zones were innervated by the narrow process of neurohypophysis. The acidophilic prolactin cells (PRL) and adrenocorticotropic cells (ACTH) were observed in the RPD which were stained with acid fuchsin and orange G by respectively. The basophilic gonadotrophs (GTH) and thyrotrophs (TSH) were distributed in the middle PPD and reacted positively to aniline blue, periodic acid Schiff’s (PAS) and aldehyde fuchsin (AF) stain. The only acidophilic somatotrophs (STH) cells were also distributed in between GTH in PPD region which were positively stained with PAS and acid fuchsin. The GTH and TSH cells exhibited both quantitative and qualitative variations during the testicular cycle. The seasonal changes in the testes of N. notopterus have been described according to the variations in GSI values and frequency percentages of the different germ cells occurring in the testicular lobules. The sequential changes of GTH and TSH cells and testicular activities were correlated well during different reproductive phases.
The adsorption of bilirubin by Sephadex and its relationship to the criteria for exchange transfusion.

Small quantities of bilirubin (BR) were adsorbed by specially preparedh small columns of Sephadex G-25 from artificially jaundiced sera. The amount of bilirubin adsorbed from sera with bilirubin-albumin molar ratios between 1 and 2 was linearly related to the bilirubin concentration of the serum. There was no demonstrable adsorption of bilirubin by the column at ratios below 1. Sephadex columns may be of value in determining the time when exchange transfusion should be performed to prevent kernicterus.
Adaptive response and dose-response plateaus for initiation in a state-vector model of carcinogenesis.

PURPOSE To investigate whether it is possible to explain dose-response plateaus for in-vitro X-ray irradiation of different cell lines with radioprotective mechanisms such as radiologically induced expression of scavengers and repair enzymes. MATERIALS AND METHODS A biomathematical model was developed based on a previous state-vector model. New features of the model are a mathematical description of enhanced repair and radical scavenging as a result of irradiation. RESULTS The model produces a plateau in the dose-response for in-vitro tranformations between 0.5 and 1 Gy and for chromosome aberrations and it predicts an inverse-fractionation effect within a selected range of doses. CONCLUSIONS Adaptive response mechanisms within a state-vector model provide a coherent explanation of the dose-response characteristics for in-vitro transformations and chromosomal aberrations. These results suggest the need for new experimental studies described in the paper.
Supporting health systems in Europe: added value of EU actions?

Abstract Since the start of the economic crisis, the European Union's (EU's) predominant discourse has been austerity and fiscal consolidation. The detrimental effects on Europe's health systems and the health status of its citizens are well described. However, little is known about the emerging EU-level initiatives to support national health systems handle the challenges of efficient care provision and system reorganisation aimed to meet their future needs. This review analyses the manner, conditions and prospects of such EU support. First, health system objectives are increasingly entering the EU health policy agenda. Second, professional and patient mobility provisions may support member states (MS) in copying with crisis related health challenges but can potentially acerbate them at the same time. Third, in recent initiatives health system goals are more closely tied to the EU's economic growth narrative. And fourth, health system issues are taken up in existing EU-level structures for debate and exchange between MS. In addition, the design of some policies may have the potential to intensify socioeconomic and health inequalities rather than ameliorate them.
Non‐uniform conduction time in the olivocerebellar pathway in the anaesthetized cat.

1. It has recently been demonstrated that conduction velocities of cerebellar climbing fibre afferents in the rat are tuned according to fibre length such that conduction time between their origin in the inferior olive and their target cortical Purkinje cells is constant. Here we have examined the situation in the cat, where individual climbing fibres are substantially longer. Complex spike responses of Purkinje cells located at various depths in the vermis (zones a and b) were evoked by electrical stimulation of olivocerebellar fibres close to their origin and were recorded either extra‐ or intracellularly. 2. The onset latencies of directly evoked complex spikes ranged from 2.6 to 6.9 ms. A consistent trend in each electrode penetration was that the complex spike latencies were longer for the superficially encountered cells (where olivocerebellar fibre length is greatest) and shorter for deeper cells (where olivocerebellar fibre length is shorter). 3. Linear regression analysis suggests that conduction time in olivocerebellar fibres in the cat is not fixed but varies linearly with conduction distance. Our findings would be consistent with a uniform conduction velocity in olivocerebellar fibres of about 6.6 m s‐1.
Circulating CD14+CD16+ monocyte levels predict tissue invasive character of cholangiocarcinoma

Chronic inflammation as a risk factor for cancer development is driven in part by monocyte/macrophages, which in many cancers exhibit pro‐tumorigenic activity. In this study we identified elevation in CD14+CD16+, a minor blood monocyte subpopulation in cholangiocarcinoma (CCA) patients, compared to normal and biliary disease patient specimens. Tumour association was suggested by the observation that this elevated level decreased to normal after tumour resection. Moreover, the elevated level of CD14+CD16+ monocytes in CCA patient blood correlated with degree of MAC387‐positive (recent blood‐derived macrophage migrant‐specific marker) tumour‐associated macrophage infiltration as determined by immunohistochemistry. These CD14+CD16+ monocytes were suggested to enhance tumour progression as this subpopulation possesses (i) high expression of adhesion molecules (CD11c, CD49d, and CD54) and scavenger receptor (CD163), which enable them to adhere strongly to endothelial cells, and (ii) that peripheral blood monocytes from CCA patients express high levels of growth and angiogenic factor‐related genes (epiregulin, VEGF‐A and CXCL3). Elevation of peripheral CD14+CD16+ monocyte levels was associated with features associated with poor prognosis CCA parameters (non‐papillary type and high number of tissue macrophages). These data indicate that the CD14+CD16+ monocytes from CCA patients with pro‐tumorigenic characteristics may associate with rapid tumour progression and poor patient outcome. If confirmed in subsequent studies, the level of CD14+CD16+ monocytes may serve as a marker for disease activity in CCA patients and serve as a target for pathogenic macrophage specific drug development.
Rational polygons as rotation sets of generic homeomorphisms of the two torus

We prove the existence of an open and dense set 𝔇⊂Homeo0(𝕋2) (where Homeo0(𝕋2) denotes the set of toral homeomorphisms homotopic to the identity) such that the rotation set of any element in 𝔇 is a rational polygon. We also extend this result to the set of axiom A diffeomorphisms in Homeo0(𝕋2). Further, we observe the existence of minimal sets whose rotation set is a non‐trivial segment, for an open set in Homeo0(𝕋2).
Idealized tropical cyclone simulations of intermediate complexity: A test case for AGCMs

The paper introduces a moist, deterministic test case of intermediate complexity for Atmospheric General Circulation Models (AGCMs). We suggest pairing an AGCM dynamical core with simple physical parameterizations to test the evolution of a single, idealized, initially weak vortex into a tropical cyclone. The initial conditions are based on an initial vortex seed that is in gradient‐wind and hydrostatic balance. The suggested “simple‐physics” package consists of parameterizations of bulk aerodynamic surface fluxes for moisture, sensible heat and momentum, boundary layer diffusion, and large‐scale condensation. Such a configuration includes the important driving mechanisms for tropical cyclones, and leads to a rapid intensification of the initial vortex over a forecast period of ten days. The simple‐physics test paradigm is not limited to tropical cyclones, and can be universally applied to other flow fields. The physical parameterizations are described in detail to foster model intercomparisons. The characteristics of the intermediate‐complexity test case are demonstrated with the help of four hydrostatic dynamical cores that are part of the Community Atmosphere Model version 5 (CAM 5) developed at the National Center for Atmospheric Research (NCAR). In particular, these are the Finite‐Volume, Spectral Element, and spectral transform Eulerian and semi‐Lagrangian dynamical cores that are coupled to the simple‐physics suite. The simulations show that despite the simplicity of the physics forcings the models develop the tropical cyclone at horizontal grid spacings of about 55 km and finer. The simple‐physics simulations reveal essential differences in the storm's structure and strength due to the choice of the dynamical core. Similar differences are also seen in complex full‐physics aqua‐planet experiments with CAM 5 which serve as a motivator for this work. The results suggest that differences in complex full‐physics simulations can be, at least partly, replicated in simplified model setups. The simplified experiments might therefore provide easier access to an improved physical understanding of how the dynamical core and moist physical parameterizations interact. It is concluded that the simple‐physics test case has the potential to close the gap between dry dynamical core assessments and full‐physics aqua‐planet experiments, and can shed light on the role of the dynamical core in the presence of moisture processes.
CD8+ LYMPHOCYTES AUGMENT CHRONIC REJECTION IN A MHC CLASS II MISMATCHED MODEL

Chronic rejection, or cardiac allograft vasculopathy (CAV), remains the leading cause of late death in heart transplant recipients. The precise role and contributions of T lymphocyte subsets to CAV development remains unknown. Methods. Donor hearts from B6.C-H2bm12 mice were transplanted into T lymphocyte subset knockout recipients and T lymphocyte-reconstituted nude recipients. No immunosuppression was used. Intimal proliferation was measured morphometrically. In vitro studies were performed to analyze the donor-specific activation status of recipient CD8+ lymphocytes by examining cellular proliferation, interleukin-2 secretion, and interleukin-2R&agr; expression. Intracellular cytokine staining assay was performed to determine both the profile and source of intragraft cytokines. Results. Hearts transplanted into wild-type recipients developed severe CAV by 24 days. Intimal lesions were absent in the hearts that were transplanted into nude and CD4−/− knockout mice (containing CD8+ lymphocytes). In contrast, the donor hearts in CD8−/− knockout recipients (containing CD4+ lymphocytes) developed CAV, but significantly less than in wild-type. Adoptive transfer of T lymphocyte subset populations into nude recipients confirmed that CAV was absolutely contingent on CD4+ lymphocytes, and that CD8+ lymphocytes played an additive role in intimal lesion progression in the presence of CD4+ lymphocytes. Although CD8+ lymphocytes alone did not cause CAV in vivo, we demonstrated that MHC class II disparate alloantigens activated CD8+ lymphocytes both in vivo and in vitro. Finally, both CD4+ and CD8+ lymphocytes contributed to the intragraft IL-2 and IFN-&ggr; production. Conclusions. In this MHC class II mismatched murine model, CAV is a T lymphocyte dependent event, and absolutely contingent on the presence of CD4+ lymphocytes. Furthermore, CD8+ lymphocytes (1) are activated by MHC class II disparate antigens and (2) play a significant role in the progression of lesion development. Finally, both CD4+ and CD8+ lymphocytes contribute to CAV development via secretion of IFN-&ggr;, a known mediator of CAV in this model.
Intraventricular interferon and oral inosiplex in the treatment of subacute sclerosing panencephalitis

We treated 22 patients with subacute sclerosing panencephalitis (SSPE) with intraventricular α-interferon (IFN) and inosiplex PO and followed them for 2 to 54 months. Three deaths occurred. Clinical improvement, demonstrated by decreasing scores on the Neurological Disability Index, occurred in 11/22 (50%); five patients became stable, and the progression rate of the disease decreased in three. The remission rate was significantly higher than untreated controls from the same institution. Patients who had a slowly progressive disease responded best to treatment. Serious side effects were rare. We recommend intraventricular IFN, combined with oral inosiplex, in the treatment of SSPE.
Adverse reaction to Althesin

A fit 48-year-old lady, with no previous history of allergy or untoward incident during anaesthesia, was anaesthetized, for dilatation and curettage and insertion of intrauterine guide tubes for radiotherapy, with 4.5 ml Althesin (54 mg total steroids), nitrous oxide, oxygen and halothane. Anaesthesia and recovery were uneventful. Two weeks later the patient was anaesthetized for further radiotherapy. Immediately following the injection of 5 ml of Althesin the patient developed a marked crimson erythematous rash covering the whole body except for areas of pressure and, simultaneously, the blood pressure and radial pulse became unrecordable although the heart sounds were present. There was no evidence of bronchospasni, but a mild generalized tissue oedema was noted. One litre of Hartmann’s solution with hydrocortisone 200 mg was administered intravenously after which the radial pulse became palpable and the systolic blood pressure could be recorded as 80 mmHg. The ECG showed no abnormality apart from a sinus tachycardia. The infusion of more intravenous fluid raised the systolic arterial pressure to 100 mmHg, anda further increase to a normal value of 120 mmHg systolic occurred over the course of the next 12 hr.
Erectile dysfunction in high-risk hypertensive patients treated with beta-blockade agents.

BACKGROUND Erectile dysfunction (ED) is a multifactorial disease related to age, vascular disease, psychological disorders, or medical treatments. Beta-blockade agents are the recommended treatment for hypertensive patients with some specific organ damage but have been outlined as one of leading causes of drug-related ED, although differences between beta-blockade agents have not been assessed. METHODS Cross-sectional and observational study of hypertensive male subjects treated with any beta-blockade agent for at least 6 months. ED dysfunction was assessed by the International Index of Erectile Dysfunction (IIEF). RESULTS 1.007 patients, mean age 57.9 (10.59) years, were included. The prevalence of any category of ED was 71.0% (38.1% mild ED; 16.8% moderate ED; 16.1% severe ED). Patients with ED had longer time since the diagnosis of hypertension and higher prevalence of risk factors and comorbidities. The prevalence of ED increased linearly with age. ED patients received more medications and were more frequently treated with carvedilol and less frequently with nebivolol. Patients treated with nebivolol obtained higher scores in every parameter of the IIEF questionnaire. The multivariate analysis identified independent associations between ED and coronary heart disease (OR: 1.57), depression (OR: 2.25), diabetes (OR: 2.27), atrial fibrillation (OR: 2.59), and dyhidopiridines calcium channel blockers (OR: 1.76); treatment with nebivolol was associated to lower prevalence of ED (OR: 0.27). CONCLUSION ED is highly prevalent in hypertensive patients treated with beta-blockade agents. The presence of ED is associated with more extended organ damage and not to cardiovascular treatments, except for the lower prevalence in nebivolol-treated patients.
The relationship between health related quality of life and disease activity and damage in systemic lupus erythematosus.

OBJECTIVE To evaluate the relationship between self-reported quality of life and disease activity, damage, impairment, disability, and handicap in systemic lupus erythematosus (SLE). METHODS In this cross sectional study disease activity was measured with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and the Systemic Lupus Activity Measure (SLAM), and damage by the Systemic Lupus International Collaborating Clinics/American College of Rheumatology (SLICC/ACR) damage index (SDI). Quality of life was assessed by the Medical Outcome Survey Short Form 36 (SF-36) and the EuroQol (EQ-5D). Multiple linear regression was used to identify significant associations of patients' health status, and logistic regression was used to evaluate the relationship of each of the 5 dimensions of the EQ-5D in terms of impairment, disability, and handicap. RESULTS Damage was associated with the Physical Function (PF) and Social Function subscales of the SF-36. Disease activity was associated with the General Health (GH) subscale. Ability to carry out usual activities was strongly related to PF and GH as well as to global rating of the thermometer rating scale of the EQ-5D. Role Physical (RP) and Bodily Pain (BP) of the SF-36 were also associated with the EQ-5D rating scale. In addition, patients' ratings of anxiety and depression were strongly related to the Mental Health (MH) summary scale of the SF-36. CONCLUSION Perceived health status of patients with lupus was associated with disease activity, damage, role physical, bodily pain, capacity for usual activity, and mobility. EQ-5D is a valid instrument for the measure of health related quality of life in SLE.