Miracle-dz/UniFAR-FTUs huggingface dataset:
Association of the HPA axis response to upcoming competition and shooting outcomes in elite junior shooting players

Abstract Successful shooting performance in competition is reliant on several factors such as shooting techniques and competition-associated psychological stresses. This study examined the hypothalamus-pituitary-adrenal (HPA) axis response to upcoming competition and its association with shooting outcomes in elite junior shooting players. The cortisol awakening response (CAR) and dehydroepiandrosterone (DHEA) secretion after awakening were measured for two consecutive days (the day before and on the day of competition for the selection of national shooting team members) in 19 junior men and 21 junior women players, and the shooting scores of the individual players were obtained. The total cortisol secretion during the CAR period (CARauc) increased, but total DHEA secretion during the post-awakening period (Daucawk) decreased on the day of competition, compared with one day before competition. The CARauc was higher in women than in men players, whereas Daucawk was higher in men than in women players across the two consecutive days. Cortisol and DHEA levels were comparable between low-scored (below the mean scores for air pistol or air rifle players) and high-scored players one day before competition. However, the CARauc on the day of competition was higher and the variations in the CARauc and molar CARauc/Daucawk ratios across the two consecutive days were greater in low-scored than in high-scored men and women players. These results indicated that upcoming competition involves alterations of the CAR and DHEA secretion after the awakening period, and greater HPA response to the upcoming competition was adversely associated with shooting scores in junior shooting athletes. Lay summary An important upcoming competition was perceived as a strong stressor on awakening that induced alteration in CAR and DHEA secretion after the awakening period in elite shooting players. This study observed that, irrespective of their age and period of shooting practice, the HPA axis function on the day of competition was associated with shooting outcomes in elite shooting players.
Plk1 protects kinetochore–centromere architecture against microtubule pulling forces

During mitosis, sister chromatids attach to microtubules which generate ~ 700 pN pulling force focused on the centromere. We report that chromatin‐localized signals generated by Polo‐like kinase 1 (Plk1) maintain the integrity of the kinetochore and centromere against this force. Without sufficient Plk1 activity, chromosomes become misaligned after normal condensation and congression. These chromosomes are silent to the mitotic checkpoint, and many lag and mis‐segregate in anaphase. Their centromeres and kinetochores lack CENP‐A, CENP‐C, CENP‐T, Hec1, Nuf2, and Knl1; however, CENP‐B is retained. CENP‐A loss occurs coincident with secondary misalignment and anaphase onset. This disruption occurs asymmetrically prior to anaphase and requires tension generated by microtubules. Mechanistically, centromeres highly recruit PICH DNA helicase and PICH depletion restores kinetochore disruption in pre‐anaphase cells. Furthermore, anaphase defects are significantly reduced by tethering Plk1 to chromatin, including H2B, and INCENP, but not to CENP‐A. Taken as a whole, this demonstrates that Plk1 signals are crucial for stabilizing centromeric architecture against tension.
In vivo identification of arteries and veins using two‐photon excitation elastin autofluorescence

Distinguishing arteries from veins in vivo has a great significance in clinical practices and preclinical studies. Optical imaging methods such as two‐photon microscopy can provide high‐resolution morphological information of tissue and are therefore extremely suitable for imaging small blood vessels. However, few optical imaging methods allow in vivo identification of arteries and veins merely utilizing the autofluorescence signal of blood vessels. In this report, we found the arterial wall generates a remarkably stronger two‐photon excitation autofluorescence (TPEA) signal compared with the venous wall based on BALB/c mice. According to histological analysis and fluorescence characteristic measurement, the contrast signal is confirmed to be from elastin fibers. Employing this unique feature, we propose an objective and effective artery–vein separation strategy that considers the presence of the elastin‐TPEA border as the indicator of arteries. Using this strategy, the arterial and venous networks of the dorsal skin and cerebral cortex of BALB/c mice are demonstrated to be excellently mapped and accurately separated in vivo without depending on any exogenous contrast agent, empirical knowledge, and algorithm. This study may provide a novel technique for mapping arterial and venous networks for anatomic research as well as an extra aid to basic researches on the mechanism, diagnosis, and treatment of blood vessel‐related diseases.
The bloom is (slightly) off the rose: the motherhood effect on psychological functioning in successive pregnancies

Abstract Objective: To examine the maternal psychological state during the course of two successive pregnancies. Methods: The sample consisted of 73 women drawn from a larger maternal–fetal cohort that participated during two pregnancies. Women completed self-report psychological questionnaires at 24, 30, and 36 weeks gestation to index maternal depressive symptoms, trait anxiety, and pregnancy hassles and uplifts. Analyses examined stability of maternal symptoms across successive pregnancies in the same women. Results: Antenatal symptoms of depression and anxiety exhibited strong intra-individual stability between successive pregnancies. Mean differences in maternal symptoms were not detected for either at 24, 30, or 36 weeks gestation, excepting elevated anxiety symptoms at the mid-point due to greater fluctuation in maternal anxiety during the prior pregnancy. Subsequent pregnancies were associated with less intense uplifting feelings about the pregnancy on each measurement occasion. Conclusions: Findings suggest marked consistency in maternal psychological orientation across subsequent pregnancies, though parity also plays a role in the maternal experience.
Towards large-scale nonparametric scene parsing of images and video

In computer vision, scene parsing is the problem of labelling every pixel in an image or video with its semantic category. Its goal is a complete and consistent semantic interpretation of the structure of the real world scene. Scene parsing forms a core component in many emerging technologies such as self-driving vehicles and prosthetic vision, and also informs complementary computer vision tasks such as depth estimation. This thesis presents a novel nonparametric scene parsing framework for images and video. In contrast to conventional practice, our scene parsing framework is built on nonparametric search-based label transfer instead of discriminative classification. We formulate exemplar-based scene parsing for both 2D (from images) and 3D (from video), and demonstrate accurate labelling on standard benchmarks. Since our framework is nonparametric, it is easily extensible to new categories and examples as the database grows. Nonparametric scene parsing is computationally demanding at test time, and requires methods for searching large collections of data that are time and memory efficient. This thesis also presents two novel binary encoding algorithms for large-scale approximate nearest neighbor search: the bank of random rotations is data independent and does not require training, while the supervised sparse projections algorithm targets efficient search of high-dimensional labelled data. We evaluate these algorithms on standard retrieval benchmarks, and then demonstrate their integration into our nonparametric scene parsing framework. Using 256-bit codes, binary encoding reduces search times by an order of magnitude and memory requirements by three orders of magnitude, while maintaining a mean per-class accuracy within 1% on the 3D scene parsing task.
Manipulation of Synechocystis sp. PCC 6803 as a platform for functional identification of genes involved in carotenoid metabolism

Carotenoids are not only essential light-harvesting/photoprotective pigments for plants, but also essential phytonutrients for human health. Although the carotenoid metabolic pathway is generally shared by photosynthetic organisms, some unique carotenoids are only synthesized in particular species. Eludication of the enzymes that catalyze their biosynthesis is a prerequisite for manipulating their production by transgenic and synthetic biological approaches. Both pigment complementation in Escherichia coli and gene overexpression in the wild-type and corresponding silencing mutants of Arabidopsis thaliana have been used for functional characterization of putative enzymes but with some limits. In this study, we engineered the carotenoid metabolic pathway of the cyanobacterium Synechocystis sp. PCC6803 by silencing its endogenous carotene hydroxylase gene (CrtO) and introducing different exogenous genes. With the zeaxanthin epoxidase gene from A. thaliana, we extended the metabolic pathway from zeaxanthin to violaxanthin in Synechocystis. We also established in Synechocystis the metabolic branch from lycopene to lutein, which does not naturally exist in cyanobacteria and some algal phyla, by transforming with the genes for lycopene ε-cyclase (Lut2) and carotene ε-hydroxylase (Lut1) from A. thaliana. Our results also confirmed that the endogenous carotene β-hydroxylase CrtR could only hydroxylate the unsubstituted β-ring. Taken together, we demonstrated that Synechocystis can be manipulated as a new platform for the functional characterization of unknown proteins that have putative functions on carotenoid metabolism. This article is protected by copyright. All rights reserved.
Singlet-Triplet Inversion in Heptazine and in Polymeric Carbon Nitrides.

According to Hund's rule, the lowest triplet state (T1) is lower in energy than the lowest excited singlet state (S1) in closed-shell molecules. The exchange integral lowers the energy of the triplet state and raises the energy of the singlet state of the same orbital character, leading to a positive singlet-triplet energy gap (∆ST). Exceptions are known for biradicals and charge-transfer excited states of large molecules in which the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO) are spatially separated, resulting in a small exchange integral. In the present work, we discovered with ADC(2), CC2, EOM-CCSD and CASPT2 calculations that heptazine (1,3,4,6,7,9,9b-heptaazaphenalene or tri-s-triazine) exhibits an inverted S1/T1 energy gap (∆ST ≈ - 0.25 eV). This appears to be the first example of a stable closed-shell organic molecule exhibiting S1/T1 inversion at its equilibrium geometry. The origins of this phenomenon are the nearly pure HOMO-LUMO excitation character of the S1 and T1 states and the lack of spatial overlap of HOMO and LUMO due to a unique structure of these orbitals of heptazine. The S1/T1 inversion is found to be extremely robust, being affected neither by substitution of heptazine nor by oligomerization of heptazine units. Using time-resolved photoluminescence and transient absorption spectroscopy, we investigated the excited-state dynamics of 2,5,8-tris(4-methoxyphenyl)-1,3,4,6,7,9,9b-heptaazaphenalene (TAHz), a chemically stable heptazine derivative, in the presence of external heavy atom sources as well as triplet-quenching oxygen. These spectroscopic data are consistent with TAHz singlet excited-state decay in the absence of a low-energy triplet loss channel. The absence of intersystem crossing and an exceptionally low radiative rate result in unusually long S1 lifetimes (of the order of hundreds of nanoseconds in non-aqueous solvents). These features of the heptazine chromophore have profound implications for organic optoelectronics as well as for water-splitting photocatalysis with heptazine-based polymers (e.g., graphitic carbon nitride) which have yet to be systematically explored and exploited.
The role of platelets in bleeding in patients with thrombocytopenia and hematological disease

Abstract This review evaluates the role of platelets in bleeding risk among patients with hematological disease and thrombocytopenia. Platelets are pivotal in primary hemostasis, and possess non-hemostatic properties involved in angiogenesis, tissue repair, inflammation and metastatis. Also, platelets safeguard vascular integrity in inflamed vessels. Overall, bleeding risk depends on the underlying disease, and patients with cancer and platelet count <6–10 × 109/L have a markedly increased bleeding risk, while the platelet count does not correlate with bleeding risk at higher platelet counts. Other factors might affect platelet properties and thus bleeding risk, for example, drugs, low hematocrit, coagulation system impairments or transfusion of dysfunctional donor platelets. For patients with leukemia and immune thrombocytopenia, reduced platelet activation, platelet aggregation, or thrombopoiesis, reflected by the reduced presence of reticulated platelets, are associated with bleeding phenotype. However, mechanistic insight into the cause of reduced platelet function in different thrombocytopenic conditions is sparse, except for some inherited platelet disorders. Promising tools for platelet function studies in thrombocytopenia are flow cytometry and biomarker studies on platelet constituents. An important message from this current paper is that bleeding risk assessment must be tailored to specific patient populations and cannot be applied broadly to all patients with thrombocytopenia.
Racial Disparities in Breastfeeding Initiation and Duration Among U.S. Infants Born in 2015

Surveillance of U.S. breastfeeding duration and exclusivity has historically reported estimates among all infants, regardless of whether they had initiated breastfeeding. These surveillance estimates have consistently shown that non-Hispanic black (black) infants are less likely to breastfeed, compared with other racial/ethnic groups. * Less is known about disparities in breastfeeding duration when calculated only among infants who had initiated breastfeeding, compared with surveillance estimates based on all infants. CDC analyzed National Immunization Survey-Child (NIS-Child) data for infants born in 2015 to describe breastfeeding duration and exclusivity at ages 3 and 6 months among all black and non-Hispanic white (white) infants, and among only those who had initiated breastfeeding. When calculated among all infants regardless of breastfeeding initiation, breastfeeding differences between black and white infants were 14.7 percentage points (95% confidence interval [CI] = 10.7-18.8) for any breastfeeding at age 3 months and were significantly different for both any and exclusive breastfeeding at both ages 3 and 6 months. Among only infants who had initiated breastfeeding, the magnitude of black-white differences in breastfeeding rates were smaller. This was most notable in rates of any breastfeeding at 3 months, where the percentage point difference between black and white infants was reduced to 1.2 (95% CI = -2.3-4.6) percentage points and was no longer statistically significant. Black-white disparities in breastfeeding duration result, in part, from disparities in initiation. Interventions both to improve breastfeeding initiation and to support continuation among black mothers might help reduce disparities.
Role of Bronchoscopic Techniques in the Diagnosis of Thoracic Sarcoidosis

The diagnosis of sarcoidosis relies on clinical and radiological presentation, evidence of non-caseating granulomas in histopathology and exclusion of alternative causes of granulomatous inflammation. Currently, a proper diagnosis, with a high level of confidence, is considered as key to the appropriate diagnosis and management of the disease. In this sense, this review aims to provide a brief overview on the role of bronchoscopy in the diagnosis of thoracic sarcoidosis, incorporating newer techniques to establish, including endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), transesophageal ultrasound-guided needle aspiration with the use of an echo bronchoscope (EUS-B-FNA) and transbronchial lung cryobiopsy (TBLC). Most of the literature reports the diagnostic superiority of endosonographic techniques, such as EBUS-TBNA alone or in combination with EUS-FNA, over conventional bronchoscopic modalities in diagnosing Scadding stages I and II of the disease. Moreover, TBLC may be considered a useful and safe diagnostic tool for thoracic sarcoidosis, overcoming some limitations of transbronchial lung biopsy (TBLB), avoiding more invasive modalities and being complementary to endosonographic procedures such as EBUS-TBNA.
Medication Use and Risk of Delirium in Mechanically Ventilated Patients

One of the principal complications in patients in the intensive care unit, particularly in those receiving mechanical ventilation, is medication-induced delirium. The present study aimed to intensively analyze pharmaceutical factors affecting the development of delirium in mechanically ventilated patients using the electronic health records. The present study was designed as a retrospective case–control study. The delirium group included 500 mechanically ventilated patients. The non-delirium group included 2,000 patients who were hospitalized during the same period as the delirium group and received mechanical ventilation. A total of seven types of medications (narcotic analgesics, non-narcotic analgesics, psychopharmaceuticals, sleep aid medications, anticholinergics, steroids, and diuretics), conventionally used to manage mechanical ventilation, were found to be major risk factors associated with the occurrence of delirium. Since these medications are an integral part of managing mechanically ventilated patients, prudent protocol-based medication approaches are essential to decrease the risk of delirium.
Retroviral-like determinants and functions required for dimerization of Ty1 retrotransposon RNA

ABSTRACT During replication of long terminal repeat (LTR)-retrotransposons, their proteins and genome (g) RNA assemble into virus-like particles (VLPs) that are not infectious but functionally related to retroviral virions. Both virions and VLPs contain gRNA in a dimeric form, but contrary to retroviruses, little is known about how gRNA dimerization and packaging occurs in LTR-retrotransposons. The LTR-retrotransposon Ty1 from Saccharomyces cerevisiae is an informative model for studying LTR-retrotransposon and retrovirus replication. Using structural, mutational and functional analyses, we explored dimerization of Ty1 genomic RNA. We provide direct evidence that interactions of self-complementary PAL1 and PAL2 palindromic sequences localized within the 5′UTR are essential for Ty1 gRNA dimer formation. Mutations disrupting PAL1-PAL2 complementarity restricted RNA dimerization in vitro and Ty1 mobility in vivo. Although dimer formation and mobility of these mutants was inhibited, our work suggests that Ty1 RNA can dimerize via alternative contact points. In contrast to previous studies, we cannot confirm a role for PAL3, tRNAiMet as well as recently proposed initial kissing-loop interactions in dimer formation. Our data also supports the critical role of Ty1 Gag in RNA dimerization. Mature Ty1 Gag binds in the proximity of sequences involved in RNA dimerization and tRNAiMet annealing, but the 5′ pseudoknot in Ty1 RNA may constitute a preferred Gag-binding site. Taken together, these results expand our understanding of genome dimerization and packaging strategies utilized by LTR-retroelements.
Branes and bundles through conifold transitions and dualities in heterotic string theory

Geometric transitions between Calabi-Yau manifolds have proven to be a powerful tool in exploring the intricate and interconnected vacuum structure of string compactifications. However, their role in N=1, 4-dimensional string compactifications remains relatively unexplored. In this work we present a novel proposal for transitioning the background geometry (including NS5-branes and holomorphic, slope-stable vector bundles) of 4-dimensional, N=1 heterotic string compactifications through a conifold transition connecting Calabi-Yau threefolds. Our proposal is geometric in nature but informed by the heterotic effective theory. Central to this study is a description of how the cotangent bundles of the deformation and resolution manifolds in the conifold can be connected by an apparent small instanton transition with a 5-brane wrapping the small resolution curves. We show that by a"pair creation"process 5-branes can be generated simultaneously in the gauge and gravitational sectors and used to describe a coupled minimal change in the manifold and gauge sector. This observation leads us to propose dualities for 5-branes and gauge bundles in heterotic conifolds which we then confirm at the level of spectrum in large classes of examples. While the 5-brane duality is novel, we observe that the bundle correspondence has appeared before in the Target Space Duality exhibited by (0,2) GLSMs. Thus our work provides a geometric explanation of (0,2) Target Space Duality.
CRISPR/Cas9 Editing of the Polyomavirus Tumor Antigens Inhibits Merkel Cell Carcinoma Growth In Vitro

Merkel cell carcinoma (MCC) is an aggressive type of skin cancer whose main causative agent is Merkel cell polyomavirus (MCPyV). MCPyV is integrated into the genome of the tumor cells in most MCCs. Virus-positive tumor cells constitutively express two viral oncoproteins that promote cell growth: the small (sT) and the large (LT) tumor antigens (TAs). Despite the success of immunotherapies in patients with MCC, not all individuals respond to these treatments. Therefore, new therapeutic options continue to be investigated. Herein, we used CRISPR/Cas9 to target the viral oncogenes in two virus-positive MCC cell lines: MS-1 and WAGA. Frameshift mutations introduced in the target sequence upon repair of the Cas9-induced DNA break resulted in decreased LT protein levels, which subsequently impaired cell proliferation, caused cell cycle arrest, and led to increased apoptosis. Importantly, a virus-negative non-MCC cell line (HEK293T) remained unaffected, as well as those cells expressing a non-targeting single-guide RNA (sgRNA). Thus, we presumed that the noted effects were not due to the off-target activity of the TAs-targeting sgRNAs. Additionally, WAGA cells had altered levels of cellular proteins involved in cell cycle regulation, supporting the observed cell cycle. Taken together, our findings provide evidence for the development of a CRISPR/Cas9-based therapeutic option for virus-positive MCC.