Miracle-dz/UniFAR-FTUs huggingface dataset:
The geography of rental housing discrimination, segregation, and social exclusion: New evidence from Sydney

ABSTRACT We investigate whether rental housing discrimination directed against 2 predominant ethnic minority groups in Sydney, Australia, is more likely to occur in neighborhoods with a particular mix of ethnicities, socioeconomic profiles, or quality of social goods and whether this geographic pattern reinforces spatial disadvantages of these minorities in a way that abets their social exclusion. We construct measures of differential treatment based on in-person paired testing conducted in 2013, with Anglo, Indian, and Muslim Middle Eastern testers. We summarize 4 dimensions of postcode-level social goods using a principal component analysis reflecting school quality, crime rates, resident employment rates, proximate jobs and job growth, and commuting options. Our ordinary least squares (OLS) regressions show that differential treatment in the Sydney rental market is strongly related to a neighborhood’s ethnic composition and 2 aspects of its social goods involving both desirable and undesirable components but is not related to the socioeconomic characteristics of the neighborhood’s population.
Blunt bowel and mesenteric injuries: the role of screening computed tomography.

BACKGROUND Early generation scanners have demonstrated poor sensitivity detecting blunt bowel/mesenteric injuries (BBMI). This study was aimed at determining the accuracy and role of helical scanners in BBMI. METHODS Retrospective chart review of patients with BBMI, or computed tomographic scans suspicious of BBMI, from August of 1995 to December of 1998. RESULTS One hundred of 8,112 scans (1.2%) were suspicious of BBMI. Of these suspicious scans, 53 patients had BBMI (true positive-TP) and 47 patients did not (false positive-FP). Seven patients with negative scans had BBMI (false negative-FN). Computed tomography contributed toward early surgery in 77% of patients who may have been delayed. Six patients developed intra-abdominal abscess. The abscess group had a significantly longer time interval from injury to surgery. Multiple findings were seen in 57% of true positive scans, whereas in 13% of false positive scans (p < 0.0001). An algorithm for management of BBMI is presented. CONCLUSION Helical scanners have high accuracy in detecting BBMI. Single versus multiple findings are useful in managing these injuries.
The Interaction of a Carbohydrate-binding Module from a Clostridium perfringens N-Acetyl-β-hexosaminidase with Its Carbohydrate Receptor*

Clostridium perfringens is a notable colonizer of the human gastrointestinal tract. This bacterium is quite remarkable for a human pathogen by the number of glycoside hydrolases found in its genome. The modularity of these enzymes is striking as is the frequent occurrence of modules having amino acid sequence identity with family 32 carbohydrate-binding modules (CBMs), often referred to as F5/8 domains. Here we report the properties of family 32 CBMs from a C. perfringens N-acetyl-β-hexosaminidase. Macroarray, UV difference, and isothermal titration calorimetry binding studies indicate a preference for the disaccharide LacNAc (β-d-galactosyl-1,4-β-d-N-acetylglucosamine). The molecular details of the interaction of this CBM with galactose, LacNAc, and the type II blood group H-trisaccharide are revealed by x-ray crystallographic studies at resolutions of 1.49, 2.4, and 2.3 Å, respectively.
Structural analysis of the embryonic development in Brycon cephalus (Günther, 1869)

Summary The embryogenesis of Brycon cephalus was established in seven stages: zygote, cleavage, blastula, gastrula, segmentation, larval and hatching, in an incubation period of 11 h (26°C). The zygote phase was observed directly after fertilization and egg hydration. Cleavage began at 0.5 h of incubation and extended up to the morula phase (1.5 h; +100 blastomeres). Cleavage was meroblastic and underwent the following division pattern: the first five divisions were vertical and perpendicular to each other, following the model 2 × 2, 4 × 2, 4 × 4 and 4 × 8. The sixth division was horizontal and occurred at 1.25 h after fertilization, giving rise to two cell layers (4 × 8 × 2) with 64 blastomeres. At the blastula stage (1.25–1.5 h), an irregular space between the blastomeres, the blastocoele, could be detected and the periblast structure initiated. The gastrula (1.75–6.0 h) was characterized by the morphogenetic movements of epiboly, convergence and cell involution, and formation of the embryonic axis. The segmentation stage (7–9 h) comprised the development of somites, the notochord, optic, otic and Kupffer's vesicles, neural tube, primitive intestine and ended with the release of the tail. The larval stage (up to 10 h) was characterized by the presence of 30 somites and growth and elongation of the larvae. At the hatching stage, the embryos presented more than 30 somites and exhibited swimming movements and a soft chorion. The blastomeres presented euchromatic nuclei, indicating a high mitotic activity and many yolk globules in the cytoplasm. The periblast was constituted of a layer with several nuclei and many vesicles, which grew during the epiboly movement.
Simultaneous 3D Imaging of Bone and Vessel Microstructure in a Rat Model

Analysis of bone microvascularization has generally been performed from 2D histology. The method proposed in this study enables for the first time to simultaneously analyze, in 3D, the microvascularization and bone microstructure in a rat model. The method is based on the use of quantitative synchrotron micro-computed tomography (SR-μCT) coupled to an automatic image analysis procedure. It was applied to investigate the effect of intermittent parathyroid hormone (PTH) administration on angiogenesis and osteogenesis in rats. Rats were posthumously injected with a contrast agent and subsequently imaged. The algorithm allowed the reconstruction and the segmentation of both bone microstructure and microvascularization in cortical and trabecular regions. A large set of 3D quantitative parameters were then extracted from the bone and vascular networks. In particular, we propose a new parameter, utilizing the availability of both microstructures to relate the two, which we dub the vascular-trabecular interdistance (VTI). Due to the short acquisition times of SR-μCT and the efficiency of the image analysis algorithm, a large data set was analyzed, which permitted statistical analysis of the measured parameters. Statistical analysis confirmed that treatment with PTH significantly modulated several bone and vessel parameters, including the VTI.
The Discordance of Individual Risk Estimates and the Reference Class Problem

Multivariate methods that relate outcomes to risk factors have been adopted clinically to individualize treatment. This has promoted the belief that individuals have a true or unique risk. The logic of assigning an individual a single risk value has been criticized since 1866. The reason is that any individual can be simultaneously considered a member of different groups, with each group having its own risk level (the reference class problem). Lemeshow et al. provided well-documented examples of remarkable discordance between predictions for an individual by different valid predictive methods utilizing different risk factors. The prevalence of such discordance is unknown as it is rarely evaluated, but must be substantial due to the abundance of risk factors. Lemeshow et al. cautioned against using ICU mortality predictions for the provision of care to individual patients. If individual risk estimates are used clinically, users should be aware that valid methods may give very different results.
Aspects of Bioavailability of Mercury for Methylation in Pure Cultures of Desulfobulbus propionicus (1pr3)

ABSTRACT We have previously hypothesized that sulfide inhibits Hg methylation by decreasing its bioavailability to sulfate-reducing bacteria (SRB), the important methylators of Hg in natural sediments. With a view to designing a bioassay to test this hypothesis, we investigated a number of aspects of Hg methylation by the SRBDesulfobulbus propionicus, including (i) the relationship between cell density and methylmercury (MeHg) production, (ii) the time course of Hg methylation relative to growth stage, (iii) changes in the bioavailability of an added inorganic Hg (HgI) spike over time, and (iv) the dependence of methylation on the concentration of dissolved HgI present in the culture. We then tested the effect of sulfide on MeHg production by this microorganism. These experiments demonstrated that under conditions of equal bioavailability, per-cell MeHg production was constant through log-phase culture growth. However, the methylation rate of a new Hg spike dramatically decreased after the first 5 h. This result was seen whether methylation rate was expressed as a fraction of the total added Hg or the filtered HgI concentration, which suggests that Hg bioavailability decreased through both changes in Hg complexation and formation of solid phases. At low sulfide concentration, MeHg production was linearly related to the concentration of filtered HgI. The methylation of filtered HgI decreased about fourfold as sulfide concentration was increased from 10−6 to 10−3 M. This decline is consistent with a decrease in the bioavailability of HgI, possibly due to a decline in the dissolved neutral complex, HgS0.
Genetic and pharmacological analyses of Syk function in alphaIIbbeta3 signaling in platelets.

Agonists induce inside-out IIbβ3signaling resulting in fibrinogen binding and platelet aggregation. These in turn trigger outside-in signaling resulting in further platelet stimulation. Because the Syk tyrosine kinase is activated during both phases of integrin signaling, we evaluated its role in IIbβ3 function in murine platelets rendered null for Syk by gene targeting and in human platelets incubated with piceatannol, a tyrosine kinase inhibitor reportedly selective for Syk. Both Syk null murine platelets and piceatannol-treated human platelets exhibited a partial, but statistically significant defect in activation of IIbβ3 by adenine diphosphate (ADP) ± epinephrine as assessed by fibrinogen binding. Syk null platelets adhered normally to immobilized fibrinogen, and mice with these platelets exhibited normal tail bleeding times. In contrast, piceatannol treatment of human platelets completely inhibited platelet adhesion to immobilized fibrinogen. The discrepancy in extent of integrin dysfunction between murine and human platelet models may be due to lack of specificity of piceatannol, because this compound inhibited the activity of Src and FAK as well as Syk and also reduced tyrosine phosphorylation of multiple platelet proteins. These results provide genetic evidence that Syk plays a role in IIbβ3 signaling in platelets and pharmacological evidence that, although piceatannol also inhibits IIbβ3 signaling, it does so by inhibtion of multiple protein tyrosine kinases.
Psychometric properties of the Posttraumatic Diagnostic Scale for DSM-5 (PDS-5).

[Correction Notice: An Erratum for this article was reported in Vol 28(10) of Psychological Assessment (see record 2016-36101-001). In the article, the third sentence of the Internal Consistency subsection of the Results section should read: Item 8, “Not being able to remember important parts of the trauma,” had an item–total correlation of . 34, and Item 16, “Taking more risks or doing things that might cause you or others harm” had an item–total correlation of . 44; the range of item–total correlations for the remaining 18 items was . 62–. 82, with an average of . 70. ”] The aim of this study was to evaluate the psychometric properties of the Posttraumatic Diagnostic Scale for DSM–5 (PDS–5), a self-report measure of posttraumatic stress disorder (PTSD) based on diagnostic criteria of the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders. Participants were 242 urban community residents, veterans, and college undergraduates recruited from 3 study sites who had experienced a DSM–5 Criterion A traumatic experience. The PDS–5 demonstrated excellent internal consistency (α = .95) and test–retest reliability (r = .90) and good convergent validity with the PTSD Checklist—Specific Version (r = .90) and the PTSD Symptom Scale—Interview Version for DSM–5 (PSSI–5; r = .85). The PDS–5 also showed good discriminant validity with the Beck Depression Inventory—II and the State–Trait Anxiety Inventory—Trait scale (all ZH > 3.05, ps < .01). There was 78% agreement between the PDS–5 and the PSSI–5. Receiver operating characteristic analysis yielded a cutoff score of 28 for identifying a probable PTSD diagnosis. The PDS–5 is a valid and reliable measure of DSM–5 PTSD symptomatology.
MARCH1 E3 Ubiquitin Ligase Dampens the Innate Inflammatory Response by Modulating Monocyte Functions in Mice

Ubiquitination was recently identified as a central process in the pathogenesis and development of numerous inflammatory diseases, such as obesity, atherosclerosis, and asthma. Treatment with proteasomal inhibitors led to severe side effects because ubiquitination is heavily involved in a plethora of cellular functions. Thus, new players regulating ubiquitination processes must be identified to improve therapies for inflammatory diseases. In addition to their role in adaptive immunity, endosomal MHC class II (MHCII) molecules were shown to modulate innate immune responses by fine tuning the TLR4 signaling pathway. However, the role of MHCII ubiquitination by membrane associated ring-CH-type finger 1 (MARCH1) E3 ubiquitin ligase in this process remains to be assessed. In this article, we demonstrate that MARCH1 is a key inhibitor of innate inflammation in response to bacterial endotoxins. The higher mortality of March1−/− mice challenged with a lethal dose of LPS was associated with significantly stronger systemic production of proinflammatory cytokines and splenic NK cell activation; however, we did not find evidence that MARCH1 modulates LPS or IL-10 signaling pathways. Instead, the mechanism by which MARCH1 protects against endotoxic shock rests on its capacity to promote the transition of monocytes from Ly6CHi to Ly6C+/−. Moreover, in competitive bone marrow chimeras, March1−/− monocytes and polymorphonuclear neutrophils outcompeted wild-type cells with regard to bone marrow egress and homing to peripheral organs. We conclude that MARCH1 exerts MHCII-independent effects that regulate the innate arm of immunity. Thus, MARCH1 might represent a potential new target for emerging therapies based on ubiquitination reactions in inflammatory diseases.
Effect of the fatty acid composition of acclimated oenological Lactobacillus plantarum on the resistance to ethanol

The aim of this work was to evaluate the changes due to acclimation to ethanol on the fatty acid composition of three oenological Lactobacillus plantarum strains and their effect on the resistance to ethanol and malic acid consumption (MAC). Lactobacillus plantarum UNQLp 133, UNQLp 65.3 and UNQLp 155 were acclimated in the presence of 6 or 10% v/v ethanol, for 48 h at 28°C. Lipids were extracted to obtain fatty acid methyl esters and analysed by gas chromatography interfaced with mass spectroscopy. The influence of change in fatty acid composition on the viability and MAC in synthetic wine was analysed by determining the Pearson correlation coefficient. Acclimated strains showed a significant change in the fatty composition with regard to the nonacclimated strains. Adaptation to ethanol led to a decrease in the unsaturated/saturated ratio, mainly resulting from an increase in the contribution of short‐length fatty acid C12:0 and a decrease of C18:1. The content of C12:0 was related to a higher viability after inoculation of synthetic wine. The MAC increased at higher contents in saturated fatty acid, but its efficiency was strain dependent.
Early Versus Delayed Operative Intervention in Displaced Clavicle Fractures

Objectives: Recent evidence to suggest that fixation of clavicle fractures yields better outcomes than conservative treatments has led to an increasing trend toward operative management. There is no evidence, however, to compare early fixation with delayed fixation for symptomatic patients before union. Design: Prospective comparative case series. Setting: Level 1 regional trauma center. Patients: Displaced clavicle fractures treated operatively in our institution during a 4-year period. Ninety-seven patients were included: 68 with early fixation and 29 delayed. Radiographic and clinical outcomes were available for all patients and scores were available for 62. Intervention: Early plate fixation (within 3 weeks) of displaced clavicle fractures compared with delayed (3–12 weeks) fixation of displaced clavicle fractures. Outcomes: Radiographic union, Oxford Shoulder Score, QuickDASH, EQ5D, and a patient interview. Mean follow-up was to 30 months. Results: There were no statistically significant differences in age (P > 0.05), sex (P > 0.05), and energy of injury (P > 0.05) between the 2 groups. The mean QuickDASH was 8.9 early and 9.1 delayed (P < 0.05) and the Oxford Shoulder Score was 44.2 early and 43.9 delayed (P < 0.05). In the early fixation group, there were 5 wound healing complications, and 8 went on subsequently to have removal of prominent metalwork. In the delayed fixation group, 2 had wound healing complications and 4 required removal of prominent metalwork. There were no statistically significant differences in the EQ5D scores. Conclusion: Our series supports delayed fixation of symptomatic clavicle fractures as results do not differ from early fixation. Level of Evidence: Therapeutic Level II. See Instructions for Authors for a complete description of levels of evidence.
The fusiform response to faces: Explicit versus implicit processing of emotion

Regions of the fusiform gyrus (FG) respond preferentially to faces over other classes of visual stimuli. It remains unclear whether emotional face information modulates FG activity. In the present study, whole‐head magnetoencephalography (MEG) was obtained from fifteen healthy adults who viewed emotionally expressive faces and made button responses based upon emotion (explicit condition) or age (implicit condition). Dipole source modeling produced source waveforms for left and right primary visual and left and right fusiform areas. Stronger left FG activity (M170) to fearful than happy or neutral faces was observed only in the explicit task, suggesting that directed attention to the emotional content of faces facilitates observation of M170 valence modulation. A strong association between M170 FG activity and reaction times in the explicit task provided additional evidence for a role of the fusiform gyrus in processing emotional information. Hum Brain Mapp, 2013. © 2011 Wiley Periodicals, Inc.
Genetic diversity among isolates of Phaeomoniella chlamydospora on grapevines

Phaeomoniella chlamydospora is one of the main causal agents of Petri disease and esca of grapevines. Although it is known to have a coelomycete synanamorph, no teleomorph has thus far been reported for P. chlamydospora, and its disease cycle remains largely unknown. The present study compared the genetic diversity of P. chlamydospora isolates from different grapevine-growing countries using amplified fragment length polymorphisms. Sixty-three isolates from South Africa and 25 from grapevine regions in Australia, France, Iran, Italy, New Zealand, Slovenia and the USA were studied. Two primer combinations were used producing 138 scorable markers, of which 33% were polymorphic. An unweighted paired group method of arithmetic averages analysis showed a high similarity (≥94.5%) among the different isolates. The overall low level of genetic variation confirmed asexual reproduction to be dominant in the field. Different genotypes were found among isolates of P. chlamydospora within the same grapevine, suggesting multiple infections from different inoculum sources. Isolates from different production areas and countries had a high percentage of similarity and clustered together, indicating the absence of genotype-geographic structure. The presence of the same genotype in different vineyards and production areas suggests that long-range dispersal through aerial inoculum or infected plant material play an important role in genotype distribution.
Cross Regional Differences in HIV/AIDS Prevalence in Tanzania: How Socioeconomic and Cultural Contexts Affect Perceived Individual and Group Efficacy

The purpose of this study was to conduct a cross-regional comparative analysis of the cultural, social, and economic differences that influence the HIV/AIDS prevalence as well as the differences in media channels and messages used in the prevention campaigns in two neighboring regions in Tanzania-Singida with one of the lowest (3.3%) and Iringa with one of the highest (9.1%) prevalence in the country. Four focus groups were conducted in each region among both rural and urban areas in the two regions. The findings indicated major differences in tribal composition, cultural, social, and economic factors that influence sexual behaviors in the two regions. Participants also indicated that there were differences in media channels used in the prevention campaigns in the two regions. The findings have practical and theoretical implications for campaign designers, scholars, organizations, and funding agencies in Tanzania in particular and sub-Saharan Africa in general.
Depressive symptoms as a predictor of cognitive decline: MacArthur Studies of Successful Aging.

OBJECTIVE The prevalence of dementia continues to rise, and yet, there are few known modifiable risk factors. Depression, as a treatable condition, may be important in the development of dementia. Our objective was to examine the association between depressive symptoms and longitudinal cognitive changes in older adults who were high-functioning at baseline. METHODS The authors analyzed data from a community-based cohort (aged 70-79 at baseline), who, at study entry, scored 7 or more (out of 9) on the Short Portable Mental Status Questionnaire (SPMSQ). Depressive symptoms were assessed at baseline using the depression subscale of the Hopkins Symptom Check List. Cognitive performance was measured at baseline and at seven-year follow up by the SPMSQ and by summary scores from standard tests of naming, construction, spatial recognition, abstraction, and delayed recall. RESULTS After adjusting for potential confounders, including age, education, and chronic health conditions such as diabetes, heart attack, stroke, and hypertension, a higher number of baseline depressive symptoms were strongly associated with greater seven-year decline in cognitive performance and with higher odds of incident cognitive impairment, i.e., decline in SPMSQ score to < or = 6 (adjusted odds ratio per quartile of depressive symptoms score: 1.34, 95% confidence interval: 1.10-1.68). CONCLUSIONS Depressive symptomatology independently predicts cognitive decline and incident cognitive impairment in previously high-functioning older persons.