Miracle-dz/UniFAR-FTUs huggingface dataset:
From Early Family Systems to Internalizing Symptoms: The Role of Emotion Regulation and Peer Relations

Research has demonstrated the importance of early family characteristics, such as the quality of caregiving, on children’s later mental health. Information is, however, needed about the role of more holistic family systems and specific child-related socioemotional mechanisms. In this study, we conceptualize families as dynamic family system types, consisting of both marital and parenting trajectories over the transition to parenthood. First, we examine how early family system types predict children’s anxiety, depression, peer exclusion, and emotion regulation. Second, we test whether couples’ infertility history and other family related contextual factors moderate the effects of family system types on child outcomes. Third, we test whether children’s emotion regulation and peer exclusion mediate the effects of family system types on anxiety and depression. The participants were 452 families representing cohesive, distant, authoritative, enmeshed, and discrepant family types, identified on the basis of relationship autonomy and intimacy from pregnancy to the child’s age of 2 and 12 months. Children’s anxiety, depression, emotion regulation, and peer exclusion were assessed at the age of 7–8 years. Structural equation modeling showed that distant, enmeshed, and discrepant families similarly predicted children’s heightened anxiety and depression. Infertility history, parental education, and parity moderated the associations between certain family system types and child outcomes. Finally, emotion regulation, but not peer exclusion, was a common mediating mechanism between distant and enmeshed families and children’s depression. The results emphasize the importance of early family environments on children’s emotion regulation development and internalizing psychopathology.
Function of Rta Is Essential for Lytic Replication of Murine Gammaherpesvirus 68

ABSTRACT Rta, encoded primarily by open reading frame 50, is well conserved among gammaherpesviruses. It has been shown that the Rta proteins of Epstein Barr virus (EBV), Kaposi's sarcoma-associated herpesvirus (KSHV, or HHV-8), and murine gammaherpesvirus 68 (MHV-68; also referred to as γHV68) play an important role in viral reactivation from latency. However, the role of Rta during productive de novo infection has not been characterized in gammaherpesviruses. Since there are cell lines that can support efficient productive de novo infection by MHV-68 but not EBV or KSHV, we examined whether MHV-68 Rta plays a role in initiating viral lytic replication in productively infected cells. Rta, functioning as a transcriptional activator, can activate the viral promoter of early lytic genes. The amino acid sequence alignments of the Rta homologues suggest that the organizations of their functional domains are similar, with the DNA binding and dimerization domains at the N terminus and the trans-activation domain at the C terminus. We constructed two mutants of MHV-68 Rta, Rd1 and Rd2, with deletions of 112 and 243 amino acids from the C terminus, respectively. Rd1 and Rd2 could no longer trans-activate the promoter of MHV-68 gene 57, consistent with the deletions of theirtrans-activation domains at the C terminus. Furthermore, Rd1 and Rd2 were able to function as dominant-negative mutants, inhibiting trans-activation of wild-type Rta. To study whether Rd1 and Rd2 blocked viral lytic replication, purified virion DNA was cotransfected with Rd1 or Rd2 into fibroblasts. Expression of viral lytic proteins was greatly suppressed, and the yield of infectious viruses was reduced up to 104-fold. Stable cell lines constitutively expressing Rd2 were established and infected with MHV-68. Transcription of the immediate-early gene, rta, and the early gene, tk, of the virus was reduced in these cell lines. The presence of Rd2 also led to attenuation of viral lytic protein expression and virion production. The ability of Rta dominant-negative mutants to inhibit productive infection suggests that the trans-activation function of Rta is essential for MHV-68 lytic replication. We propose that a single viral protein, Rta, governs the initiation of MHV-68 lytic replication during both reactivation and productive de novo infection.
On the operationalization of psychopathy: further support for a three‐faceted personality oriented model

Objective: This study is an attempt to compare two alternative models of psychopathy (PCL‐R); (i) the traditional 17‐item two‐factor model where the first factor describes a deceitful, manipulative and callous, unemotional dimension and the second factor describes the impulsive, irresponsible and antisocial behavioral lifestyle dimension; and (ii) a recently proposed 13‐item three‐factor model involving an interpersonal facet, an affective facet and a behavioral facet.
Application of the boundary contour/feature contour system to magnetic resonance brain scan imagery

Boundary contour system (BCS) and feature contour system (FCS) human vision models are applied to magnetic resonance brain scan imagery to highlight significant features for the purpose of pattern recognition. A discussion is presented of psychophysical and neurophysiological evidence in support of the model. The implementation is described. The resulting images are compared to the original to demonstrate the effectiveness of the model
Risk Assessment Studies: Detailed Host Range Testing of Wild-Type Cabbage Moth, Mamestra brassicae (Lepidoptera: Noctuidae), Nuclear Polyhedrosis Virus

The host range of a multiply enveloped nuclear polyhedrosis virus (NPV) (Baculoviridae) isolated from the cabbage moth, Mamestra brassicae (Lepidoptera: Noctuidae), was determined by challenging a wide range of insect species with high (106 polyhedral inclusion bodies) and low (103 polyhedral inclusion bodies) doses of the virus. The identity of the progeny virus was confirmed by dot blotting. Analysis of 50% lethal dose was carried out on selected species, and the progeny virus was identified by using restriction enzyme analysis and Southern blotting. Other than the Lepidoptera, none of the species tested was susceptible to M. brassicae NPV. Within the Lepidoptera, M. brassicae NPV was infective to members of four families (Noctuidae, Geometridae, Yponomeutidae, and Nymphalidae). Of 66 lepidopterous species tested, M. brassicae NPV was cross-infective to 32 of them; however, 91% of the susceptible species were in the Noctuidae. The relevance of host range data in risk assessment studies is discussed.
Antisense inhibition of urokinase: Effect on malignancy in a human osteosarcoma cell line

Expression of urokinase‐type plasminogen activator (u‐PA) strongly correlates with a malignant tumor cell phenotype. In the multistep process of metastasis, different cellular functions are influenced by urokinase. The enzyme is known to be effective via both proteolytical and signal transduction mechanisms. In the present study, the osteosarcoma cell line MNNG/HOS was transfected with a vector capable of expressing an antisense transcript, complementary to 1,021 bases of the 3′ end of u‐PA cDNA. This construct was most effective in reducing u‐PA expression in previous experiments. Stably transfected antisense (as) cell lines were characterized and compared with the parental MNNG/HOS. Antisense transfection of MNNG/HOS gave the following results: (1) stable incorporation of the construct into the genome of as‐clones, as detected by Southern blot analysis; (2) decreased mRNA level of u‐PA, as detected by Northern blot analysis; (3) approximately 50% reduced enzyme expression in cell culture medium and cell homogenate; and (4) unchanged cellular proliferation activity and u‐PAR expression. In further functional analysis, as‐clones showed (1) significantly reduced invasion and motility in modified Transwell chambers (random migration and chemotaxis with collagen I as a chemoattractant); (2) significantly reduced adhesion on matrices of collagen I and vitronectin; (3) unchanged adhesion properties on Matrigel matrix; and (4) reduced metastatic potential to lungs and especially liver in chick embryos after i.v. infection into chorioallantoic membrane veins. Our data show that in MNNG/HOS urokinase influences cellular malignancy by promoting migration and selective adhesion. These specific functions were notable in addition to the effects on invasion and basement membrane degradation. Int. J. Cancer 77:153–160, 1998. © 1998 Wiley‐Liss, Inc.
Tobacco use in Vietnam. Prevalence, predictors, and the role of the transnational tobacco corporations.

OBJECTIVE To describe tobacco use in Vietnam and the impact of transnational tobacco corporations there. DESIGN In cities, a multistage cluster design; in communes, a systematic sample design, using face-to-face interviews in all sites. SETTING Hanoi, Ho Chi Minh City, and 2 rural communes in Vietnam. PARTICIPANTS Random samples totaling 2004 men and women aged 18 years or older. MAIN OUTCOME MEASURES Prevalence and correlates of tobacco smoking, amount and duration of smoking, age at initiation, quitting behavior, knowledge of health hazards of and attitudes toward smoking, and cigarette brand smoked, preferred, and recognized as most widely advertised. RESULTS Smoking prevalence among men (n=970) was 72.8% and 4.3% among women (n=1031). Male smokers had smoked a mean of 15.5 years; the median age at initiation was 19.5 years. Among male smokers, 16% smoked non-Vietnamese cigarettes. More than twice as many (38%), however, said that they would prefer to smoke a non-Vietnamese brand if they could afford the cost. Among those who recalled any cigarette advertising (38%), 71% recalled a non-Vietnamese brand as the most commonly advertised. Male smokers who were significantly more likely to smoke non-Vietnamese brands lived in the south, were engaged in blue collar or business/service occupations, earned higher incomes, and lived in urban areas. CONCLUSIONS Vietnam has the highest reported male smoking prevalence rate in the world. Unless forceful steps are taken to reduce smoking among men and prevent the uptake of smoking by youth and women, Vietnam will face a tremendous health and economic burden in the near future. Implementation of a comprehensive national tobacco control campaign together with international regulation will be the keys to the eradication of the tobacco epidemic in Vietnam and throughout the developing world.
Alternative Chromatin Structures of the 35S rRNA Genes in Saccharomyces cerevisiae Provide a Molecular Basis for the Selective Recruitment of RNA Polymerases I and II

ABSTRACT In all eukaryotes, a specialized enzyme, RNA polymerase I (Pol I), is dedicated to transcribe the 35S rRNA gene from a multicopy gene cluster, the ribosomal DNA (rDNA). In certain Saccharomyces cerevisiae mutants, 35S rRNA genes can be transcribed by RNA polymerase II (Pol II). In these mutants, rDNA silencing of Pol II transcription is impaired. It has been speculated that upstream activating factor (UAF), which binds to a specific DNA element within the Pol I promoter, plays a crucial role in forming chromatin structures responsible for polymerase specificity and silencing at the rDNA locus. We therefore performed an in-depth analysis of chromatin structure and composition in different mutant backgrounds. We demonstrate that chromatin architecture of the entire Pol I-transcribed region is substantially altered in the absence of UAF, allowing RNA polymerases II and III to access DNA elements flanking a Pol promoter-proximal Reb1 binding site. Furthermore, lack of UAF leads to the loss of Sir2 from rDNA, correlating with impaired Pol II silencing. This analysis of rDNA chromatin provides a molecular basis, explaining many phenotypes observed in previous genetic analyses.
Reduction of Dicer impairs Schwann cell differentiation and myelination

The process of Schwann cell myelination requires precisely coordinated gene expression. At the onset of myelination, there is an increase in the expression of differentiation‐promoting transcription factors that regulate key Schwann cell genes. Further control of myelin gene expression occurs at the posttranscriptional level and, in part, is mediated by RNA binding proteins and micro‐RNAs (miRNAs). miRNAs are small, endogenously derived RNA molecules that repress gene expression by specifically binding to their mRNA targets. In the experiments described here, we tested whether miRNAs were essential in controlling myelination by reducing the levels of Dicer, an essential endoribonuclease in miRNA biogenesis. We decreased the expression of Dicer by about 60% within Schwann cells using a lentiviral vector expressing an shRNA against Dicer. The reduced levels of Dicer led to a decrease in the steady‐state expression of selected miRNAs and of the transcription factors Oct6 and Egr2/Krox20, both of which are critical for Schwann cells differentiation and myelination. In contrast, the levels of c‐jun and Sox2 were up‐regulated by the reduction in Dicer and were associated with an increase in Schwann cell proliferation. In dorsal root ganglion cocultures, Schwann cells transduced with Dicer shRNA synthesized less myelin, which was accompanied by significant reductions in the levels of myelin basic protein and protein zero. These findings support a critical role for Dicer and miRNAs in Schwann cell differentiation and myelination. © 2010 Wiley‐Liss, Inc.
Severe keratitis following corneal cross‐linking for keratoconus

performed and the patient underwent proton beam radiotherapy. Two years after proton beam radiotherapy, the tumour thickness was 3.5 mm but the patient developed a recurrent vitreous haemorrhage and neovascular glaucoma requiring enucleation. Pathological examination showed a partially necrotic choroidal melanoma of epithelioid cell type, with vascular hyalinization from the radiotherapy. The mitotic count was 1 ⁄ 40 high power fields. Numerous macrophages were seen. The sclera adjacent to the tumour showed collagen degeneration and calcification (Fig. 2). Apical tumour recurrence after ruthenium plaque radiotherapy is rare, even with eccentric plaque placement (Damato et al. 2005; Lommatzsch et al. 2000; Summanen et al. 1993). This case therefore raises the possibility that treatment failure occurred because of intrascleral calcification beneath the tumour. We suggest that this finding should prompt a higher dose of brachytherapy or an alternative form of radiotherapy, such as proton beam radiotherapy.
Choking Under the Pressure of a Positive Stereotype: Gender Identification and Self-Consciousness Moderate Men's Math Test Performance

ABSTRACT Choking under pressure occurs when an individual underperforms due to situational pressure. The present study examined whether being the target of a positive social stereotype regarding math ability causes choking among men. Gender identification and self-consciousness were hypothesized to moderate the effect of math-gender stereotypes on men's math test performance. Men high in self-consciousness but low in gender identification significantly underperformed when exposed to gender-relevant test instructions. No significant effects were found under a gender-irrelevant condition. These findings are discussed in the contexts of research on stereotype threat, stereotype lift, and choking under pressure.
Individual genetic diversity and probability of infection by avian malaria parasites in blue tits (Cyanistes caeruleus)

Understanding the importance of host genetic diversity for coping with parasites and infectious diseases is a long‐standing goal in evolutionary biology. Here, we study the association between probability of infection by avian malaria (Plasmodium relictum) and individual genetic diversity in three blue tit (Cyanistes caeruleus) populations that strongly differ in prevalence of this parasite. For this purpose, we screened avian malaria infections and genotyped 789 blue tits across 26 microsatellite markers. We used two different arrays of markers: 14 loci classified as neutral and 12 loci classified as putatively functional. We found a significant relationship between probability of infection and host genetic diversity estimated at the subset of neutral markers that was not explained by strong local effects and did not differ among the studied populations. This relationship was not linear, and probability of infection increased up to values of homozygosity by locus (HL) around 0.15, reached a plateau at values of HL from 0.15 to 0.40 and finally declined among a small proportion of highly homozygous individuals (HL > 0.4). We did not find evidence for significant identity disequilibrium, which may have resulted from a low variance of inbreeding in the study populations and/or the small power of our set of markers to detect it. A combination of subtle positive and negative local effects and/or a saturation threshold in the association between probability of infection and host genetic diversity in combination with increased resistance to parasites in highly homozygous individuals may explain the observed negative quadratic relationship. Overall, our study highlights that parasites play an important role in shaping host genetic variation and suggests that the use of large sets of neutral markers may be more appropriate for the study of heterozygosity–fitness correlations.
A wideband, directional model for the satellite‐to‐indoor propagation channel at S‐band

This paper presents a hybrid empirical‐statistical model for the satellite‐to‐indoor propagation channel at S‐band derived from measurements using a helicopter to simulate the satellite. The measurements have been carried out by means of a wideband, circularly polarized channel sounder in a SIMO (Single Input Multiple Output) configuration, allowing the derivation of entry loss, wideband and spatial model parameters. It is hoped that this paper will provide relevant information on the satellite‐to‐indoor channel given the large amount of experimental data available, covering a significant member of different buildings of various types, and the amount of measurement configurations: elevations and azimuths. Copyright © 2009 John Wiley & Sons, Ltd.
Primary children's attitudes towards electronic games and effect of electronic games on primary children's mathematics learning

Electronic game playing is a very popular activity for children today. In the last few years there has been much attention to the potential of using games for learning. Though there have been some negative sides to electronic game playing – such as the claim that game playing is linked to aggressive or addictive behaviours (Sandford & Williamson, 2005) – a number of empirical studies suggest that games can be a tool for learning (e.g. McFarlane, Sparrowhawk & Heald, 2002, Miller & Robertson, 2010, 2011). The purpose of this thesis was to investigate children’s attitudes towards electronic games in Scotland and China and examine the effects of mathematics electronic games on the mathematics achievement and mathematics attitudes of primary school students. In the first part of the research, a total of 44 students from one primary school in Scotland and 127 pupils from two primary schools in China participated in the study investigating their attitude towards electronic games. This study found that electronic game playing was a very popular activity for both Scottish and Chinese children and they had positive attitudes towards electronic games. Children were motivated by the fun aspects most. However Scottish children spent more time on gaming than Chinese students. Moreover, Scottish children tended to regard games primarily as a source of enjoyment and for entertainment, while games seemed to be a learning medium besides fun for Chinese students. The second part of the study examined the effects of a mobile phone game ‘Brain challenge’ on Primary 4 students’ achievement in mathematics and on students’ attitudes towards mathematics. An experimental control-group design with repeated
Mobility of the Lower Lumbar Spine After Posterolateral Fusion Determined by Roentgen Stereophotogrammetric Analysis

To determine the time-table for intervertebral stabilization after posterolateral fusion without osteosynthesis in the lower lumbar spine, 11 patients with no previous spinal surgery and with fusion due to spondylolysis-olisthesis Grade 1 to 2 or lumbar disc disorder/facet joint arthrosis were examined by roentgen stereophotogrammetric analysis (RSA) in supine and erect positions, and by conventional radiography for 1 year after surgery. In eight patients with osseous fusion radiographically, the sagittal and the vertical translations between the fused vertebral segments began to decrease after 3 to 6 months. However, the time for rigid fusion as defined by RSA varied between 3 months and 1 year, and in four patients sagittal/vertical translations of mostly less than 1 mm still persisted at 1 year postoperatively. In three patients with poor fusion radiographically, no rigid fusion as defined by RSA was obtained. Sagittal/vertical translations of 1 to 10 mm persisted at 1 year postoperatively in these patients. The preoperative pain disappeared in all patients except in one who had osseous fusion radiographically but persisting translations after 1 year.
On the Probabilistic and Physical Consistency of Traffic Random Variables and Models

In this article we deal with the probabilistic and physical consistency of traffic‐related random variables and models. We analyze and discuss the conditions for a model to be consistent from two different points of view: probabilistic and physical (dimensional analysis). The first, leads us to the concept of stability in general and reproductivity in particular because, for example, origin–destination (OD) and link flows are the sum of route flows and route travel times are the sum of link travel times. This implies stability with respect to sums (reproductivity). Normal models are justified because when the number of summands increases the averages approach the normal distribution. Similarly, stability with respect to minimum or maximum operations arises in practice. From the dimensional analysis point of view, some models are demonstrated not to be convenient. In particular, it is shown that some families of distributions are valid only for dimensionless variables. All these and other problems are discussed and some proposed models in the literature are analyzed from these two points of view. When some families fail to satisfy the desired properties, alternative models are provided via extension of the original families. Finally, some simple examples and conclusions are given to summarize the analysis.
A Stability Notion for Lattice Boltzmann Equations

Lattice Boltzmann equations are usually constructed to satisfy some physical requirements such as Galilean invariance and isotropy, to possess a velocity-independent pressure and no compressible effects, and so on. In this paper, a stability requirement is introduced as a new criterion for the constructions. With this requirement, we derive some relations of parameters for several lattice Boltzmann models. Interestingly, these relations are satisfied by many choices of parameters used in the literature.