Miracle-dz/UniFAR-FTUs huggingface dataset:
A.S.P.E.N. clinical guidelines: nutrition support of adult patients with hyperglycemia.

BACKGROUND Hyperglycemia is a frequent occurrence in adult hospitalized patients who receive nutrition support. Both hyperglycemia and hypoglycemia (resulting from attempts to correct hyperglycemia) are associated with adverse outcomes in diabetic as well as nondiabetic patients. This American Society for Parenteral and Enteral Nutrition (A.S.P.E.N.) Clinical Guideline summarizes the most current evidence and provides guidelines for the desired blood glucose goal range in hospitalized patients receiving nutrition support, the definition of hypoglycemia, and the rationale for use of diabetes-specific enteral formulas in hospitalized patients. METHOD A systematic review of the best available evidence to answer a series of questions regarding glucose control in adults receiving parenteral or enteral nutrition was undertaken and evaluated using concepts adopted from the Grading of Recommendations, Assessment, Development and Evaluation working group. A consensus process was used to develop the clinical guideline recommendations prior to external and internal review and approval by the A.S. P.E. N. Board of Directors. RESULTS/CONCLUSIONS 1. What is the desired blood glucose goal range in adult hospitalized patients receiving nutrition support? We recommend a target blood glucose goal range of 140-180 mg/dL (7.8-10 mmol/L). (Strong) 2. How is hypoglycemia defined in adult hospitalized patients receiving nutrition support? We recommend that hypoglycemia be defined as a blood glucose concentration of <70 mg/dL (<3.9 mmol/L). (Strong) 3. Should diabetes-specific enteral formulas be used for adult hospitalized patients with hyperglycemia? We cannot make a recommendation at this time.
Estimation of the light shift in Ramsey-coherent population trapping

We both numerically and analytically investigate on the light shift in Ramsey-CPT resonance for compact atomic clocks. The numerical calculation of the light shift is based on a density matrix analysis in Λ-type three state model. And the analytical method leads to an estimation equation of light shift in the Raman-Ramsey scheme. The estimation equation expresses the relationship between light shift and all pulse parameters. The results show that the estimation equation was reproduced well as the numerical results.
Effect of inhibition of the electrogenic Na+/K+ pump on the mechanical activity in the rat uterus

Summary— The effects of ouabain and K+‐free solution were studied in estrogen‐primed rat uterine strips under resting tone or repeatedly stimulated with KCl, acetylcholine or oxytocin applied for 20 minutes at 60 minute intervals. These effects were compared with those of the K+ channel opener cromakalim. In preparations under resting tone, ouabain (0.1 mM and 0.3 mM) induced rhythmic contractions which disappeared after 20–30 minutes whereas at a higher concentration (1 mM) it evoked a rapid, phasic response followed by a small tonic contraction. Exposure of the strip to a K+‐free solution induced either rhythmic waves, which ceased after 8–10 minutes, or a single phasic contraction which was followed by a small and slow increase in the resting tone (54 ± 10 mg after 180 min exposure). Nifedipine (0.3 μM) abolished the rhythmic or phasic component of these responses but failed to modify the late small tonic contraction induced by ouabain 1 mM or by K+‐free solution. Ouabain (0.1–1 mM) or K+‐free‐evoked responses disappeared after short (4 min) or prolonged (60 min) exposure to a Ca2+‐free, 3 mM EGTA‐containing solution. Cromakalim (10 nM −0.1 mM) did not induce any variation in the resting tone either in the presence or in the absence of Ca2+ in the medium. In strips repeatedly stimulated with acetylcholine (0.1 mM) or oxytocin (1 μM), ouabain (0.3 mM), K+‐free‐solution and cromakalim (10 μM) reduced the amplitude of the initial, phasic response and progressively decreased the oscillatory component of the response to these agonists. Conversely, the successive responses evoked by KCl 60 mM in similar experimental conditions were not affected by ouabain or cromakalim. Ouabain (0.3 mM), K+‐free solution and cromakalim (10 μM) decreased the Ca2+‐independent, maintained contractions induced by acetylcholine or oxytocin after prolonged exposure to a Ca2+‐free, EGTA‐containing medium. These inhibitory effects were partially or completely reversed in the presence of the non‐selective potassium channel blocker tetraethylammonium (10 mM) or in a Ca2+‐free solution containing 60 mM K+. In conclusion, these results suggest that the response induced by ouabain or K+‐free solution in estrogen‐primed rat myometrium involves Ca2+ influx through potential‐operated calcium channels but not Ca2+ release from intracellular stores. In addition, our results show that prolonged exposure to ouabain or K+‐free medium decreases membrane receptor‐mediated responses in rat uterus. This inhibitory effect seems to be the result, at least in part, of a decrease in the cytosolic level of K+, due to the inhibition of the electrogenic Na+ pump.
Impaired Recycling of Apolipoprotein E4 Is Associated with Intracellular Cholesterol Accumulation*

After internalization of triglyceride-rich lipoproteins (TRL) in hepatoma cells, TRL particles are immediately disintegrated in the early endosomal compartment. This involves the targeting of lipids and apoprotein B along the degradative pathway and the recycling of TRL-derived apoE through recycling endosomes. Re-secretion of apoE is accompanied by the concomitant association of apoE and cellular cholesterol with high-density lipoproteins (HDL). Since epidemiological data showed that apoE3 and apoE4 have differential effects on HDL metabolism, we investigated whether the intracellular processing of TRL-derived apoE4 differs from apoE3-TRL. In this study, we demonstrated by radioactive and immunofluorescence uptake experiments that cell-surface binding and internalization of TRL-derived apoE4 are increased compared with apoE3 in hepatoma cells. Pulse-chase experiments revealed that HDL-induced recycling, but not disintegration and degradation, of apoE4-enriched TRL is strongly reduced in these cells. Furthermore, impaired HDL-induced apoE4 recycling is associated with reduced cholesterol efflux. Studies performed in Tangier fibroblasts showed that apoE recycling does not depend on ATP-binding cassette transporter A1 activity. These studies provide initial evidence that impaired recycling of apoE4 could interfere with intracellular cholesterol transport and contribute to the pathophysiological lipoprotein profile observed in apoE4 homozygotes.
Solid-phase enzyme immunoassay for Chlamydial antibodies

An enzyme immunoassay (EIA) for chlamydial immunoglobulin G antibodies was developed by using microtiter wells coated with partially purified reticulate bodies of Chlamydia trachomatis serotype L2, grown in McCoy cells, and uninfected McCoy cells as a control. Duplicate testing of a single serum dilution, 1:500, was found to be sufficient. A good correlation between positive reactions was observed in a comparative study of 421 patient sera with the EIA and an inclusion immunofluorescence test. A good correlation between positive reactions was also observed in a comparative study of 140 patient sera with EIA and microimmunofluorescence tests in which chlamydial elementary or reticulate bodies were used as antigens. Sera of 77 healthy control individuals with low titers in inclusion immunofluorescence or complement fixation tests gave negative results in the EIA. Immunoblotting experiments showed that the major antigenic component in the EIA antigen was a protein with an Mr of 39,000.
Proactive and Reactive Aggression in a Child Psychiatric Inpatient Population

The study examined relations between caregiver- and child-reported proactive and reactive aggression and psychopathic characteristics (callous/unemotional traits, narcissism, and impulsivity) in a sample of 105 children (69% male) ranging from 6 to 12 years of age (M = 9.61, SD = 1.83) who were admitted to a child psychiatric inpatient facility. Caregiver reports of aggression suggested that proactive and reactive aggression were similarly related to callous/unemotional traits and narcissism but that only reactive aggression was associated with impulsivity. In contrast, child reports of proactive aggression, but not reactive aggression, were associated with all three psychopathic characteristics. Theoretical and clinical implications are discussed.
Development and implementation of Timor-Leste health policy framework 2002 : a governance analysis

This thesis examines the role of the central and local administration (the Ministry of Health and District Health Service) and their development partners who are tasked with the implementation of health services with the attendant relevant health policies within the bounds of the Timor-Leste’s Health Policy Framework 2002. This study explores and analyzes how health policy actors, decision makers and implementers engage with policy processes and elements of governance. The elements include: structure, power, equity, ethics, cooperation, responsiveness, transparency, accountability, effectiveness and efficiency, and participation. The research explores structures and processes that are in place, the way they operate, and their legitimacy. It compares these arrangements in two districts, where one district has good performance and one district has poor performance in terms of achieving Ministry of Health identified health indicators and objectives. The study identifies that these elements of governance are essential to strengthen local health governance and to improve the health status and well-being of the Timorese people. The overall design of this study followed mixed-methods, qualitative and quantitative approaches with case studies. NVIVO 8 computer software program was used to facilitate data analysis and assist with interpretation. It was identified that the two districts have similarities and differences in history and experience of governance, as well as geography, demography, socio economic and human capital background. These have contributed to differences in performance and differences in the achievement of health indicators and objectives. It was found that the development of health policies and procedures was dominated by those with power, including donors and leaders of the political parties, which disempowered the local administration and communities. Lack of responsiveness on the part of the central and local administration and partners is the foremost concern of both districts. This lack of responsiveness jeopardizes the implementation of health programs.
Hip fracture risk in older white men is associated with change in body weight from age 50 years to old age.

BACKGROUND Change in body weight is a potentially modifiable risk factor for hip fracture in older women but, to our knowledge, its relationship to risk in older men has not been reported previously. OBJECTIVE To investigate the effects of weight loss and weight gain from age 50 years to old age on the risk of hip fracture among elderly men. METHODS The association between weight change and risk of hip fracture was studied in a cohort of 2413 community-dwelling white men aged 67 years or older from 3 sites of the Established Populations for Epidemiologic Study of the Elderly. RESULTS The older men in this study, observed for a total of 13620 person-years during the 8 years of follow-up, experienced 72 hip fractures, yielding an overall incidence rate of 5.3 per 1000 person-years. Extreme weight loss (> or =10%) beginning at age 50 years was associated in a proportional hazards model with increased risk of hip fracture (relative risk, 1.8; 95% confidence interval, 1.04-3.3). Weight loss of 10% or more was associated with several indicators of poor health, including physical disability, low mental status score, and low physical activity (P<.05). Weight gain of 10% or more beginning at age 50 years provided borderline protection against the risk of hip fracture (relative risk, 0.4; 95% confidence interval, 0.1-1.00). CONCLUSIONS Despite differences between older men and women in the incidence of and risk factors for hip fracture, weight history is also an important determinant of the risk of hip fracture among older men. Weight loss of 10% or more beginning at age 50 years increases the risk of hip fracture in older white men; weight gain of 10% or more decreases the risk of hip fracture. The relationship between extreme weight loss and poor health suggests that weight loss is a marker of frailty that may increase the risk of hip fracture in older men. Physicians should include weight history in their assessment of the risk of hip fracture among older men.
Genomic Subtraction Identifies Salmonella typhimurium Prophages, F-Related Plasmid Sequences, and a Novel Fimbrial Operon, stf, Which Are Absent inSalmonella typhi

ABSTRACT Salmonella typhimurium causes systemic and fatal infection in inbred mice, while the related serotype Salmonella typhi is avirulent for mammals other than humans. In order to identify genes from the virulent strain S. typhimurium ATCC 14028 that are absent in S. typhi Ty2, and therefore might be involved in S. typhimurium mouse virulence, a PCR-supported genomic subtractive hybridization procedure was employed. We have identified a novel putative fimbrial operon,stfACDEFG, located at centisome 5 of the S. typhimurium chromosome, which is absent in S. typhi,Salmonella arizonae, and Salmonella bongori but was detected in several other Salmonella serotypes. The fimbrial genes represent a genomic insertion in S. typhimurium compared to the respective region betweenfhuB and hemL in Escherichia coliK-12. In addition, the subtraction procedure yielded F plasmid-related sequences from the S. typhimurium virulence plasmid, a number of DNA fragments representing parts of lambdoid prophages and putative sugar transporters, and several fragments with unknown sequences. The majority of subtracted chromosomal sequences map to three distinct locations, around centisomes 5, 27, and 57.
Borrowing Challenges for Minorities: How Lending Technologies Affect Discrimination in the SME Credit Market

Can relationship-lending technology be used to overcome discrimination, as the Allport’s (1954) contact hypothesis suggests? Or should minorities stay away from the bank? Using a sample of SMEs from the Survey of Small Business Finances 2003, this article shows that though relationship-lending technology generally reduces the spread and probability of the need to provide collateral for a loan, use of such technology actually leads to greater discrimination against minorities. Asians are the most negatively affected by this effect, followed by Afro-Americans and Hispanics. However, this effect occurs only when the length of the relationship is short; it tends to disappear when relationships are longer. With regard to the area in which a firm is located, there is less discrimination in areas with high minority population rates than in areas with low minority population rates. These results are robust with regard to the self-selection problem.
Genetic modifiers of EGFR dependence in non-small cell lung cancer

Significance Non-small cell lung cancers (NSCLCs) harboring mutations in the epidermal growth factor receptor (EGFR) gene are often singularly reliant on EGFR activity for tumor cell survival, but the genetic basis for this dependence is not fully understood. In this study, we have performed a screen to identify a spectrum of kinase genes whose overexpression can overcome NSCLC cells’ reliance on EGFR. Using both unbiased and targeted approaches, we demonstrate that these genes commonly bypass dependence on EGFR through reactivation of downstream signaling pathways. Lung adenocarcinomas harboring activating mutations in the epidermal growth factor receptor (EGFR) represent a common molecular subset of non-small cell lung cancer (NSCLC) cases. EGFR mutations predict sensitivity to EGFR tyrosine kinase inhibitors (TKIs) and thus represent a dependency in NSCLCs harboring these alterations, but the genetic basis of EGFR dependence is not fully understood. Here, we applied an unbiased, ORF-based screen to identify genetic modifiers of EGFR dependence in EGFR-mutant NSCLC cells. This approach identified 18 kinase and kinase-related genes whose overexpression can substitute for EGFR in EGFR-dependent PC9 cells, and these genes include seven of nine Src family kinase genes, FGFR1, FGFR2, ITK, NTRK1, NTRK2, MOS, MST1R, and RAF1. A subset of these genes can complement loss of EGFR activity across multiple EGFR-dependent models. Unbiased gene-expression profiling of cells overexpressing EGFR bypass genes, together with targeted validation studies, reveals EGFR-independent activation of the MEK-ERK and phosphoinositide 3-kinase (PI3K)-AKT pathways. Combined inhibition of PI3K-mTOR and MEK restores EGFR dependence in cells expressing each of the 18 EGFR bypass genes. Together, these data uncover a broad spectrum of kinases capable of overcoming dependence on EGFR and underscore their convergence on the PI3K-AKT and MEK-ERK signaling axes in sustaining EGFR-independent survival.
CEACAM1 (CD66a) Promotes Human Monocyte Survival via a Phosphatidylinositol 3-Kinase- and AKT-dependent Pathway*

CEACAM1 (also known as CD66a) is a transmembrane glycoprotein that mediates homophilic intercellular interactions that influence cellular growth, immune cell activation, and tissue morphogenesis. Various studies have suggested a link between CEACAM1 and cellular apoptosis, including a recent demonstration that ERK1/2 signaling is triggered downstream of CEACAM1. In this study, we reveal that CEACAM1-long binding confers survival signals to human peripheral blood mononuclear cells. CEACAM-specific antibodies effectively protected peripheral blood mononuclear cells from apoptosis, with this effect being particularly dramatic for primary monocytes that undergo spontaneous apoptosis during in vitro culture. This protective effect was reiterated when using soluble CEACAM1, which binds to cell-surface CEACAM1 via homophilic interactions. Monocyte survival correlated with a CEACAM1-dependent up-regulation of the cellular inhibitor of apoptosis Bcl-2 and the abrogation of caspase-3 activation. CEACAM1 binding triggered a phosphatidylinositol 3-kinase-dependent activation of the protein kinase Akt without influencing the activity of extracellular signal-related kinase ERK, whereas the phosphatidylinositol 3-kinase-specific inhibitor LY294002 effectively blocked the protective effect of CEACAM1. Together, this work indicates that CEACAM1 confers a phosphatidylinositol 3-kinase- and Akt-dependent survival signal that inhibits mitochondrion-dependent apoptosis of monocytes. By controlling both ERK/MEK and PI3K/Akt pathways, CEACAM1 functions as a key regulator of contact-dependent control of cell survival, differentiation, and growth.
The use of albumin solid dispersion to enhance the solubility of unionizable drugs

Abstract In this study, solid dispersions of prednisolone (PRD) and bovine serum albumin (BSA) were prepared by spray drying and freeze drying methods using a PRD:BSA solution [20:1 molar ratio (MR)]. PRD-BSA dispersed mixtures were characterized by scanning electron microscopy (SEM), and powder X-ray diffraction (XRD), and differential scanning calorimetry (DSC). PRD-BSA physical and dispersed mixtures showed significantly higher solubility in water than that of unprocessed drug. Enhancement factor of six was obtained in both physical mixture and solid dispersion solubility studies. In-vitro dissolution and release studies under physiological conditions showed an immediate release of PRD from the solid dispersions, with almost 90% of the drug dissolved in the first 10 min. PRD was immediately released from BSA binding complex. This study demonstrates the potential for the use of BSA to enhance the solubility and dissolution rate, hence bioavailability, of the unionizable drugs.
A Market Neutral Statistical Arbitrage Trading Model

The momentum effect is a systematic inefficiency in the market that can be exploited by a trading strategy. This conclusion is supported by theoretical and empirical evidence. But the academic research that tries to quantify the performance of this kind of strategy often relies on a methodology that is too simplistic. The question arises what performance a trader realistically could achieve in relation to the results presented in academic journals. To answer this, we have written a computer program to run simulations with the added realism of transaction costs and more advanced trading rules based on a wider array of data than classic methodology allows. This has been done on Swedish stocks between 1995 and 2001. We then compare the simulation based on our own advanced model with a simulation that emulates a simplistic methodology. It is found that the negative impact on return of including transaction costs is outweighed by the lower risk attributed to our more advanced trading rules, as indicated by e.g. Sharpe and standard measures of risk. We can thus conclude that the momentum effect might be even more attractive as a basis for a trading strategy than have been suggested in prior academic research. As an academic paper, we think that the methodology (our simulation platform) used to obtain the conclusion in our thesis is more important than the conclusion itself. It is evident that a good evaluation of any trading strategy requires more realistic simulations than is commonplace in academia today.
Characterization of the APLF FHA–XRCC1 phosphopeptide interaction and its structural and functional implications

Abstract Aprataxin and PNKP-like factor (APLF) is a DNA repair factor containing a forkhead-associated (FHA) domain that supports binding to the phosphorylated FHA domain binding motifs (FBMs) in XRCC1 and XRCC4. We have characterized the interaction of the APLF FHA domain with phosphorylated XRCC1 peptides using crystallographic, NMR, and fluorescence polarization studies. The FHA–FBM interactions exhibit significant pH dependence in the physiological range as a consequence of the atypically high pK values of the phosphoserine and phosphothreonine residues and the preference for a dianionic charge state of FHA-bound pThr. These high pK values are characteristic of the polyanionic peptides typically produced by CK2 phosphorylation. Binding affinity is greatly enhanced by residues flanking the crystallographically-defined recognition motif, apparently as a consequence of non-specific electrostatic interactions, supporting the role of XRCC1 in nuclear cotransport of APLF. The FHA domain-dependent interaction of XRCC1 with APLF joins repair scaffolds that support single-strand break repair and non-homologous end joining (NHEJ). It is suggested that for double-strand DNA breaks that have initially formed a complex with PARP1 and its binding partner XRCC1, this interaction acts as a backup attempt to intercept the more error-prone alternative NHEJ repair pathway by recruiting Ku and associated NHEJ factors.