Miracle-dz/UniFAR-FTUs huggingface dataset:
Odd-skipped related-1 controls neural crest chondrogenesis during tongue development

Significance Development of the tongue is a major vertebrate adaptation to terrestrial life. Interestingly, although the tongues of birds and mammals initially develop similarly, the bird tongue is underpinned by an extensive internal skeleton, whereas the oral part of the mammalian tongue is boneless, which is critical not only for feeding but also for phonetic articulation and oral health. This paper shows that the mouse embryonic tongue, but not that in chick, expresses high levels of the odd-skipped related-1 (Osr1) transcription factor, which represses expression of SRY-box containing gene-9 (Sox9), the master regulator of cartilage formation. Mice lacking Osr1 function develop a cartilage in the anterior tongue reminiscent of that in chick. These data provide unique insights into the mechanisms of tongue development and evolution. The tongue is a critical element of the feeding system in tetrapod animals for their successful adaptation to terrestrial life. Whereas the oral part of the mammalian tongue contains soft tissues only, the avian tongue has an internal skeleton extending to the anterior tip. The mechanisms underlying the evolutionary divergence in tongue skeleton formation are completely unknown. We show here that the odd-skipped related-1 (Osr1) transcription factor is expressed throughout the neural crest-derived tongue mesenchyme in mouse, but not in chick, embryos during early tongue morphogenesis. Neural crest-specific inactivation of Osr1 resulted in formation of an ectopic cartilage in the mouse tongue, reminiscent in shape and developmental ontogeny of the anterior tongue cartilage in chick. SRY-box containing gene-9 (Sox9), the master regulator of chondrogenesis, is widely expressed in the nascent tongue mesenchyme at the onset of tongue morphogenesis but its expression is dramatically down-regulated concomitant with activation of Osr1 expression in the developing mouse tongue. In Osr1 mutant mouse embryos, expression of Sox9 persisted in the developing tongue mesenchyme where chondrogenesis is subsequently activated to form the ectopic cartilage. Furthermore, we show that Osr1 binds to the Sox9 gene promoter and that overexpression of Osr1 suppressed expression of endogenous Sox9 mRNAs and Sox9 promoter-driven reporter. These data indicate that Osr1 normally prevents chondrogenesis in the mammalian tongue through repression of Sox9 expression and suggest that changes in regulation of Osr1 expression in the neural crest-derived tongue mesenchyme underlie the evolutionary divergence of birds from other vertebrates in tongue morphogenesis.
Active Process Requiring TCR-Mediated -Chain Is an a Allelic Exclusion of the TCR

Phenotypic allelic exclusion at the TCR (cid:1) locus is developmentally regulated in thymocytes. Many immature thymocytes express two cell surface (cid:1) -chain species. Following positive selection, the vast majority of mature thymocytes and peripheral T cells display a single cell surface (cid:1) -chain. A posttranslational mechanism occurring at the same time as positive selection and TCR up-regulation leads to this phenotypic allelic exclusion. Different models have been proposed to explain the posttranslational regulation of the (cid:1) -chain allelic exclusion. In this study, we report that allelic exclusion is not regulated by competition between distinct (cid:1) -chains for a single (cid:2) -chain, as proposed by the dueling (cid:1) -chain model, nor by limiting CD3 (cid:3) -chain in mature TCR high thymocytes. Our data instead favor the selective retention model where the positive selection signal through the TCR leads to phenotypic allelic exclusion by speciﬁcally maintaining cell surface expression of the selected (cid:1) -chain while the nonselected (cid:1) -chain is internalized. The use of inhibitors speciﬁc for Lck and/or other Src kinases indicates a role for these protein tyrosine kinases in the signaling events leading to the down-regulation of the nonselectable (cid:1) -chain. Loss of the ubiquitin ligase/TCR signaling adapter molecule c-Cbl, which is important in TCR down-modulation and is a negative regulator of T cell signaling, leads to increased dual (cid:1) -chain expression on the cell surface of double-positive thymocytes. Thus, not only is there an important role for TCR signaling in causing (cid:1) -chain
An Estimation Method for the Semiparametric Mixed Effects Model

Summary. A semiparametric mixed effects regression model is proposed for the analysis of clustered or longitudinal data with continuous, ordinal, or binary outcome. The common assumption of Gaussian random effects is relaxed by using a predictive recursion method (Newton and Zhang, 1999) to provide a nonparametric smooth density estimate. A new strategy is introduced to accelerate the algorithm. Parameter estimates are obtained by maximizing the marginal profile likelihood by Powell's conjugate direction search method. Monte Carlo results are presented to show that the method can improve the mean squared error of the fixed effects estimators when the random effects distribution is not Gaussian. The usefulness of visualizing the random effects density itself is illustrated in the analysis of data from the Wisconsin Sleep Survey. The proposed estimation procedure is computationally feasible for quite large data sets.
Female Mate Preference in Goby Eviota prasina: Do Secondary Sexual Traits Influence Female Choice

Abstract Males of the small gobiid fish Eviota prasina have longer dorsal fins than females and use their dorsal fins for courtship displays and male-male competition. We examined the influence of male dorsal fin length as well as the frequency of courtship displays on female mate preference using sequential choice experiments. Females responded more frequently to courting males with longer dorsal fins than males with shorter dorsal fins. This indicates female mate preference on the basis of male dorsal fin length in this goby. In addition, the logistic regression analysis showed that male courtship frequency positively influenced female mate preference. Since males incur costs to produce and maintain longer dorsal fins and to perform frequent courtship displays, these secondary sexual traits may indicate the quality of physical characteristics. In E. prasina, only males conducted parental care for eggs in their nests. Therefore, females may be able to ensure greater survival rates as well as higher genetic quality of their offspring by choosing males with better qualities based on these male traits.
Early exposure to lead and neuropsychological outcome in adolescence

One hundred and ninety-five participants in the Cincinnati Lead Study were neuropsychologically evaluated in mid-adolescence. The neuropsychological measures yielded five factors labeled Memory, Learning/IQ, Attention, Visuoconstruction, and Fine-Motor. Prenatal, Average Childhood, and 78 month blood lead (PbB) levels were used in a series of multiple regression analyses. Following rigorous covariate pretesting and adjustment, a significant main effect of 78 month PbB on the Fine-Motor factor was found (p < .004). Significant interactions were also found between gender and lead exposure parameters for both Attention and Visuoconstruction indicating heightened risk in males. Finally, a trend toward significance was found for the PbB × SES interaction for Learning/IQ, consistent with previous evidence of increased educational and cognitive vulnerability for youth from more disadvantaged backgrounds. These results provide new evidence from the longest continuing prospective study of the remote effects of early lead exposure. They indicate the presence of selective neuropsychological effects in this population, and also that males and females are not uniformly affected. These results also underscore the complexity of models of neurobehavioral development, and the modest predictive power of any single determinant. (JINS, 2004, 10, 261–270.)
Elective Single Embryo Transfer: Guidelines for Practice British Fertility Society and Association of Clinical Embryologists

Assisted conception treatment is the single most important cause in the increase in multiple pregnancy and births over the last 25 years. Multiple births are associated with significant peri natal morbidity and mortality. Europe has led the way in reducing multiple births by widespread adoption of an elective single embryo policy, which in Belgium is linked to an increase in state funding. Randomized controlled trials suggest that an eSET policy must include the ability to cryopreserve and transfer any remaining quality embryos to obtain parity with a double embryo transfer. This document provides a review of the available evidence with guidelines for practice, to help facilitate the introduction of an eSET policy in the UK.
Phylogenetic analysis of the Mustela altaica (Carnivora: Mustelidae) based on complete mitochondrial genome

Abstract The mountain weasel (Mustela altaica) belongs to family Mustelidae, which is the near threatened species in the IUCN Red List. In this study, the complete mitochondrial genome of M. altaica was sequenced and characterized. The genome is 16,521 bases in length (GenBank accession no. KC815122). The nucleotide sequence data of 12 heavy-strand protein-coding genes of M. altaica and other 20 Mustelidae species were used for phylogenetic analyses. Trees constructed by using Bayesian inference, maximum parsimony and maximum likelihood demonstrated that M. altaica was close to Mustela nivalis and they were sister to Mustela putorius and Mustela sibirica.
Lithium Regulates Total and Synaptic Expression of the AMPA Glutamate Receptor GluR2 in Vitro and in Vivo

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Molecular Autopsy for Sudden Unexplained Death? Time to Discuss Pros and Cons

Molecular Autopsy for Sudden Unexplained Death? Time to Discuss Pros and Cons ANDREA MAZZANTI, M.D.∗ and SILVIA G. PRIORI, M.D., Ph.D.∗,†,‡ From the ∗Molecular Cardiology, IRCCS Fondazione Salvatore Maugeri, Pavia, Italy; †Cardiovascular Genetics Program, The Leon H. Charney Division of Cardiology, New York University School of Medicine, New York, USA; and ‡Department of Molecular Medicine, University of Pavia, Pavia, Italy
Gα12 activates Rho GTPase through tyrosine-phosphorylated leukemia-associated RhoGEF

Heterotrimeric G proteins, G12 and G13, have been shown to transduce signals from G protein-coupled receptors to activate Rho GTPase in cells. Recently, we identified p115RhoGEF, one of the guanine nucleotide exchange factors (GEFs) for Rho, as a direct link between Gα13 and Rho [Kozasa, T. , et al. (1998) Science 280, 2109–2111; Hart, M. J., et al. (1998) Science 280, 2112–2114]. Activated Gα13 stimulated the RhoGEF activity of p115 through interaction with the N-terminal RGS domain. However, Gα12 could not activate Rho through p115, although it interacted with the RGS domain of p115. The biochemical mechanism from Gα12 to Rho activation remained unknown. In this study, we analyzed the interaction of leukemia-associated RhoGEF (LARG), which also contains RGS domain, with Gα12 and Gα13. RGS domain of LARG demonstrated Gα12- and Gα13-specific GAP activity. LARG synergistically stimulated SRF activation by Gα12 and Gα13 in HeLa cells, and the SRF activation by Gα12-LARG was further stimulated by coexpression of Tec tyrosine kinase. It was also found that LARG is phosphorylated on tyrosine by Tec. In reconstitution assays, the RhoGEF activity of nonphosphorylated LARG was stimulated by Gα13 but not Gα12. However, when LARG was phosphorylated by Tec, Gα12 effectively stimulated the RhoGEF activity of LARG. These results demonstrate the biochemical mechanism of Rho activation through Gα12 and that the regulation of RhoGEFs by heterotrimeric G proteins G12/13 is further modulated by tyrosine phosphorylation.
New concept parameters of RigiScan in differentiation of vascular erectile dysfunction: Is it a useful test?

Abstract Background: The aim of this study is to investigate the value of new nocturnal penile tumescence recording parameters, such as tumescence activity unit and rigidity activity unit values, total erection number and erection times, in differentiating between psychogenic erectile dysfunction and organic erectile dysfunction. We also aimed to determine the role of these parameters in differentiating arterial erectile dysfunction from veno‐occlusive dysfunction.
Hybridoma cell culture optimization using nonlinear model predictive control

Abstract This work addresses the application of control systems to the optimization of a monoclonal antibodies (MAb) production chain. The attention is focused on the maximization of hybridoma fed-batch culture productivity. The proposed model presents kinetics showing strong nonlinearities through min-max functions expressing overflow metabolism. A nonlinear model predictive control (NMPC) algorithm, choosing the best trajectory over a moving finite horizon among different sequences of inputs, is suggested in order to optimize productivity. Sensitivities of selected objective functions are considered in a minimax robust version of the NMPC in order to choose the best configuration with respect to practical operating conditions.
A Synchronous Generator Internal Fault Model Based on the Voltage-Behind-Reactance Representation

An internal fault in a synchronous generator produces an effect similar to increasing the number of grouped coils in the stator winding, making it necessary to use additional time-variant inductances to represent the condition, with the ensuing increase in modeling complexity and computation time required for its solution. In this paper, a new model for the simulation of internal faults in synchronous generators is presented. The model is based on the so-called voltage-behind-reactance (VBR) representation, a contemporary reference frame, which has proved to be numerically more efficient than the classical phase-domain model used to study internal faults in synchronous generators; making it a better fit for large-scale, multimachine power systems applications, the long-term objective of this research work. An implementation for electromagnetic transients program (EMTP) type solutions is presented together with a test case where internal faults are applied, producing results that are in close agreement with results available in the open literature. Furthermore, an external perturbation is also carried out and results match exactly those produced by an equivalent VBR implementation.
Combination ivermectin plus diethylcarbamazine, a new effective tool for control of lymphatic filariasis.

In 1993, a three arm double-blind controlled trial was implemented in French Polynesia, to compare the tolerance and efficacy of single doses of the combination ivermectin (IVR) 400 micrograms.kg-1 plus diethylcarbamazine (DEC) 6mg.kg-1 vs IVR 400 micrograms.kg-1 or DEC 6 mg.kg-1 alone, for treatment of Wuchereria bancrofti carriers. Of the 57 treated male patients in whom microfilaremia (mf) densities ranged from 22 to 4,709 mf/ml, three groups of 19 were randomly selected, and allocated to one of the three treatments. Twelve months after treatment 37%, 16% and 16% of patients were mf negative in groups DEC, IVR and IVR plus DEC respectively. Mf percent return to pretreatment level was significantly lower in the group IVR + DEC (1.9%) than for DEC 6 (14.7%) or IVR 400 (11.6%). Antigenemia percent return to pretreatment level was lower in the groups IVR + DEC or DEC 6 than for IVR 400. The combination IVR + DEC proved to be the most effective on macrofilariae and microfilariae (antigenemia and mf negative patients). The combination will be a very powerful tool for control of lymphatic filariasis. An annual filariasis day could be the most cost-effective strategy for administration of the drugs.
Cyclin D1 (CCND1) expression is involved in estrogen receptor beta (ERβ) in human prostate cancer

Estrogen receptor beta (ERβ) has been demonstrated to be expressed in prostate carcinoma cells and estrogen signals through ERβ to act as a tumor suppressor in prostate cancer patients. ERβ is thought to regulate the cell cycle of prostate carcinoma cells by controlling the expression of cell cycle regulators including cyclin D1 (CCND1). This interaction is of particular interest as CCND1 has been implicated in the development of prostate cancer.
Two-year clinical effectiveness of a resin-modified glass-ionomer adhesive.

PURPOSE To evaluate the clinical effectiveness of a resin-modified glass-ionomer adhesive FujiBond LC (GC) in non-carious Class V cervical lesions. METHODS 28 patients and 52 abrasion-erosion lesions were restored using FujiBond LC and a microfilled resin composite in a cavity design with the adjacent enamel margins beveled. Clinical parameters as retention, marginal integrity, clinical microleakage, caries recurrence, esthetics, gingival response, tooth vitality and post-operative sensitivity were evaluated at 6 months, 1 and 2 years. RESULTS A 100% retention rate was recorded after 2 years of clinical service. However, only 15% of the restorations showed a perfect marginal adaptation. All marginal defects were small, either located at the incisal or at the cervical margin. The percentage of small marginal defects was obviously higher at the incisal enamel margin than at the cervical dentin margin. At the 2-year recall, half of the restorations exhibited a superficial, localized discoloration at the restoration margin, however none of the restorations showed deep, generalized discoloration. Remaining criteria of clinical effectiveness were rated as excellent.
Efficacy of botulinum-A toxin in adults with neurogenic overactive bladder: initial results.

INTRODUCTION AND OBJECTIVES To study the effects of intradetrusor botulinum-A toxin (BTA) (BOTOX) injections in adult patients with detrusor overactivity who failed to respond to anticholinergic medications or developed intolerable side-effects. MATERIALS AND METHODS Ten patients (average age 41 years, range 19-76 years) with neurogenic bladder and detrusor overactivity who were using clean intermittent catheterization 4 to 7 times a day with anticholinergic therapy were included in our study. Their history, physical examination, abdominal ultrasound, urine analysis, serum creatinine and electrolytes, as well as multichannel urodynamic evaluation were documented before administration of BTA injections. Anticholinergic medication was stopped at the time of injection. Three hundred to 400 U of BTA were injected into 30 to 40 different sites in the detrusor muscle under cystoscopic guidance. Clinical assessment and urodynamic testing were repeated 3 months after injection. RESULTS Five patients were tetraplegic and five were paraplegic at the thoracic or lumbar level. Three patients had incontinence episodes between catheterizations before treatment, and only one patient continued to have incontinence episodes between catheterizations after treatment. Reflex volume increased by 63.08% from 180 +/- 44.99 ml to 293.7 +/- 208.42 ml (p < 0.02). Maximal bladder capacity was augmented by 73.63% from 290 +/- 131.64 ml to 518.20 +/- 273.35 ml (p < 0.03). Maximal detrusor pressure decreased by 15.52% from 69.60 +/- 39.19 cmH(2)O to 58.80 +/- 33.95 cmH(2)O (p < 0.2). No patients complained of side effects. CONCLUSION BTA appears to be an effective and safe therapeutic option for overactive bladder in patients with spinal cord injury failing anticholinergic therapy and delays more invasive treatments. These initial results confirm European literature reports.
Domain Attention with an Ensemble of Experts

An important problem in domain adaptation is to quickly generalize to a new domain with limited supervision given K existing domains. One approach is to retrain a global model across all K + 1 domains using standard techniques, for instance Daumé III (2009). However, it is desirable to adapt without having to re-estimate a global model from scratch each time a new domain with potentially new intents and slots is added. We describe a solution based on attending an ensemble of domain experts. We assume K domain specific intent and slot models trained on respective domains. When given domain K + 1, our model uses a weighted combination of the K domain experts’ feedback along with its own opinion to make predictions on the new domain. In experiments, the model significantly outperforms baselines that do not use domain adaptation and also performs better than the full retraining approach.
Psychological effects of abortion on women: a review of the literature

The psychological effects of abortion have been the subject of scientific investigation, as well as public debate internationally, for several decades and have generated a great deal of discussion, as well as some controversy. Given the importance of this matter for women and the significant public health implications resulting from it, it is an important issue upon which to focus attention from a health policy point of view.
Dental Caries in Rats Associated with Candida albicans

In addition to occasional opportunistic colonization of the oral mucosa, Candida albicans is frequently found in carious dentin. The yeast’s potential to induce dental caries as a consequence of its pronounced ability to produce and tolerate acids was investigated. Eighty caries-active Osborne-Mendel rats were raised on an ampicillin-supplemented diet and exposed to C. albicans and/or Streptococcus mutans, except for controls. Throughout the 28-day test period, the animals were offered the modified cariogenic diet 2000a, containing 40% various sugars. Subsequently, maxillary molars were scored for plaque extent. After dissection, the mandibular molars were evaluated for smooth surface and fissure caries. Test animals exposed to C. albicans displayed considerably more advanced fissure lesions (p < 0.001) than non-exposed controls. While S. mutans yielded similar results, a combined association of C. albicans and S. mutans had no effect on occlusal caries incidence. Substituting dietary sucrose by glucose did not modify caries induction by C. albicans. However, animals fed a diet containing 20% of both sugars showed no differences to non-infected controls. Smooth surface caries was not generated by the yeast. This study provides experimental evidence that C. albicans is capable of causing occlusal caries in rats at a high rate.