Miracle-dz/UniFAR-FTUs huggingface dataset:
Ophthalmology in Critical Care.

Ophthalmic disease encountered in the intensive care unit (ICU) has a wide spectrum of prevalence and severity. Prolonged exposure of the cornea is common and preventable. Trauma, glaucoma, infection, vascular disease, and burns are among the potential causes of vision loss. Patients are predisposed to ocular complications by the ICU environment and critical illness itself. Critically ill patients require prioritization of life-sustaining interventions and less emphasis is placed on ophthalmic disease, leading to missed opportunities for vision-saving intervention. It is therefore imperative for intensivists, nurses, and other providers to have an increased awareness and understanding of the broad range of ocular conditions potentially seen in the ICU.
Evaluating and Validating Emotion Elicitation Using English and Arabic Movie Clips on a Saudi Sample

With the advancement of technology in both hardware and software, estimating human affective states has become possible. Currently, movie clips are used as they are a widely-accepted method of eliciting emotions in a replicable way. However, cultural differences might influence the effectiveness of some video clips to elicit the target emotions. In this paper, we describe several sensors and techniques to measure, validate and investigate the relationship between cultural acceptance and eliciting universal expressions of affect using movie clips. For emotion elicitation, a standardised list of English language clips, as well as an initial set of Arabic video clips are used for comparison. For validation, bio-signal devices to measure physiological and behavioural responses associated with emotional stimuli are used. Physiological and behavioural responses are measured from 29 subjects of Arabic background while watching the selected clips. For the six emotions’ classification, a multiclass SVM (six-class) classifier using the physiological and behavioural measures as input results in a higher recognition rate for elicited emotions from Arabic video clips (avg. 60%) compared to the English video clips (avg. 52%). These results might reflect that using video clips from the subjects’ culture is more likely to elicit the target emotions. Besides measuring the physiological and behavioural responses, an online survey was carried out to evaluate the effectiveness of the selected video clips in eliciting the target emotions. The online survey, having on average 220 respondents for each clip, supported the findings.
The cause of obesity: are we barking up the wrong tree?

Obesity may be more complex than most other societal problems we have faced so far. Viewed from an energy balance viewpoint, obesity can be thought of as a failure of our biological regulatory system for balancing the energy we take in from the food we eat and drinks with the energy we expend as metabolism and to perform physical activity. While some have approached obesity as resulting primarily from a ‘defect’ in the biological system itself, the problem may also be viewed as an evolving environment that is overwhelming the biological regulation of energy balance and leads to obesity. There are strong arguments for the latter point of view. First, the increase in the prevalence of obesity globally is too rapid to be associated with major genetic changes. Second, while more than 400 genes have been identified to play a role in body weight regulation, only a handful of humans worldwide have been shown to have a true ‘genetic obesity’. In the face of unidirectional environmental pressures to eat more and move less, our biological systems cannot maintain energy balance. Historically, resisting weight gain has not had great evolutional value. Put simply, for most of our history we have had no need to defend against positive energy balance. In this way, it is not our physiology that is abnormal, it is our environment. There is increasing recognition that obesity is not just a personal responsibility, but a societal issue and that dealing with it will not be simple. The factors in our environment that contribute to positive energy balance represent aspirations to which our ancestor aspired, such as having a constant supply of food and not having to work hard to secure food, shelter and safety. Obesity may be a by-product of our success as a society and it is unlikely we are willing to give up our food security and our labour-saving devices. Our challenge is not to go backwards in time to an environment that was conducive to a healthy body weight, but to learn how to modify our current environment in ways to achieve both healthy weights for our population and a high quality of life.
Basolateral 3-O-methylglucose transport by cultured kidney (LLC-PK1) epithelial cells.

In previous work we demonstrated the similarity of basolateral sugar transport of LLC-PK1 renal epithelia to basolateral kidney sugar transport using 2-deoxy-D-glucose as a substrate. In this study we first examine a central limitation to use of 2-deoxyglucose for basolateral sugar transport study in LLC-PK1 epithelia, namely, a shift of the rate-limiting step in uptake from transport to phosphorylation. Use of 3-O-methylglucose avoids this complication because it is not phosphorylated. However, use of 3-O-methylglucose requires much shorter incubation periods to examine linear rates of uptake (steady state is reached by 60 s at 22 degrees C for 0.1 mM 3-O-methylglucose). As was true for 2-deoxyglucose, apical uptake of 3-O-methylglucose was only a fraction of total uptake. Basolateral uptake was characteristically more sensitive to phloretin and cytochalasin B inhibition, relative to phlorizin. Inhibition studies indicate a requirement for a free hydroxyl on C-1 carbon of the pyranose ring, as is characteristic for renal basolateral sugar transport. Kinetic analysis indicates a single transport system with a Km of 10.9 mM and Vmax of 17.2 pmol.micrograms DNA-1.15 s-1. Subconfluent, undifferentiated LLC-PK1 cells show a similar Km (12.7 mM) but a ninefold higher Vmax (166.2 pmol.micrograms DNA-1.15 s-1). Stimulation of 3-O-methylglucose transport rate in confluent cultures by phorbol ester is relatively small (less than 100%) compared with effects on other somatic cells. The uptake rate of 3-O-methylglucose is not affected by glucose starvation, but subsequent refeeding with glucose-containing medium does significantly stimulate uptake.
Effects of long-term chlorpyrifos exposure on mortality and reproductive tissues of Banded Gourami (Trichogaster fasciata)

Abstract This study assessed the long-term toxicity of chlorpyrifos on survival and reproduction of Banded Gourami by using mortality, gonado-somatic index (GSI) and histopathological observations as endpoints. Adult fish were exposed to five different concentrations of chlorpyrifos (0, 15, 50, 150, 500 µg/L) in 15 PVC tanks for 15, 30, 45, 60 and 75 days. Results showed that all male and female fish died after 15 days of 500 µg/L chlorpyrifos exposure. No consistent significant effect was observed for both male and female GSI. Furthermore, results showed dose- and time-dependent histopathological alterations for both ovary and testes. The 60-d No Observed Effect Concentration (NOEC) for most histopathological alterations of Banded Gourami ovary and testes was 50 μg/L, while 60-d NOEC for mortality of both male and female fish was < 15 μg/L. The results show that the long-term exposure to chlorpyrifos not only affect the reproductive tissues of Banded Gourami at exposure concentrations but also cause their mortality. Future studies should evaluate effects at lower concentrations.
The Increased Effectiveness of Loaded Versus Unloaded Plyometric-Jump Training in Improving Muscle Power, Speed, Change-of-Direction, and Kicking-Distance Performance in Prepubertal Male Soccer Players.

PURPOSE This study examined the effects of loaded (LPJT) and unloaded (UPJT) plyometric jump training programmes on measures of muscle power, speed, change-of-direction and kicking-distance performance in prepubertal male soccer players. METHODS Participants (N=29) were randomly assigned to a LPJT group (n=13; age=13.0±0.7 years) using weighted vests or UPJT group (n=16; age=13.0±0.5 years) using body mass only. Before and after the intervention, tests for the assessment of proxies of muscle power (i.e., countermovement-jump [CMJ], standing-long-jump [SLJ]), speed (i.e., 5-m, 10-m, and 20-m sprint), change-of-direction (i.e., Illinois change-of-direction test [ICoDT], modified 505 agility test), and kicking-distance test were conducted. Data were analysed using magnitude-based inferences. RESULTS Within-group analyses for the LPJT group showed large and very large improvements for 10-m sprint-time (effect size [ES]=2.00) and modified 505 CoD (ES=2.83) tests, respectively. For the same group, moderate improvements were observed in ICoDT (ES=0.61), 5- and 20-m sprint-time (ES=1.00 for both tests), CMJ (ES=1.00) and MKD (ES=0.90). Small enhancements in the SLJ (ES=0.50) test were apparent. Regarding the UPJT group, small improvements were observed for all tests (ES=0.33 to 0.57) except 5-m and 10-m sprint-time (ES=1.00 and 0.63, respectively). Between-group analyses favored the LPJT group for the modified 505 CoD (ES=0.61), SLJ (ES=0.50), and MKD (ES=0.57) tests, but not for 5-m sprint-time (ES=1.00). Only trivial between-group differences were shown for the remaining tests (ES=0.00 to 0.09). CONCLUSION Overall, LPJT appears to be more effective than UPJT in improving measures of muscle power, speed, change-of-direction and kicking-distance performance in prepubertal male soccer players.
External Auditory Canal Reconstruction and Mastoid Obliteration Using Modified Palva Flap in Canal Wall Down Mastoidectomy With Tympanoplasty

Objective: To evaluate the effectiveness of a modified Palva flap used for external auditory canal reconstruction and mastoid obliteration in canal wall down mastoidectomy. Methods: We retrospectively analyzed patients who underwent canal wall down mastoidectomy with tympanoplasty using modified Palva flap. All patients underwent pure tone audiometry and temporal bone computed tomography (CT) before surgery, and the same tests were performed in the first year after surgery. The external auditory canal volumes were calculated by summing the areas of each section selected in temporal bone CT. For each patient, the ratio of external auditory canal volume was calculated from CT taken before and after surgery. Results: The mean of external auditory canal volume after canal wall down with a modified Palva flap was about 1.4 times larger than before surgery. The modified Palva flap is effective for the reconstruction of the external auditory canal. Both pure tone audiometry level and air-bone gap showed statistically significant improvement after surgery (Ps = .001 and .002, respectively). Conclusions: The external auditory canal volume slightly increased, but the status of mastoid obliteration was well maintained 1 year after surgery. The modified Palva flap used in this study is an easy and effective method in external auditory canal reconstruction and mastoid obliteration.
Cadmium Complexed with β2-Microglubulin, Albumin and Lipocalin-2 rather than Metallothionein Cause Megalin:Cubilin Dependent Toxicity of the Renal Proximal Tubule

Cadmium (Cd2+) in the environment is a significant health hazard. Chronic low Cd2+ exposure mainly results from food and tobacco smoking and causes kidney damage, predominantly in the proximal tubule. Blood Cd2+ binds to thiol-containing high (e.g., albumin, transferrin) and low molecular weight proteins (e.g., the high-affinity metal-binding protein metallothionein, β2-microglobulin, α1-microglobulin and lipocalin-2). These plasma proteins reach the glomerular filtrate and are endocytosed at the proximal tubule via the multiligand receptor complex megalin:cubilin. The current dogma of chronic Cd2+ nephrotoxicity claims that Cd2+-metallothionein endocytosed via megalin:cubilin causes renal damage. However, a thorough study of the literature strongly argues for revision of this model for various reasons, mainly: (i) It relied on studies with unusually high Cd2+-metallothionein concentrations; (ii) the KD of megalin for metallothionein is ~105-times higher than (Cd2+)-metallothionein plasma concentrations. Here we investigated the uptake and toxicity of ultrafiltrated Cd2+-binding protein ligands that are endocytosed via megalin:cubilin in the proximal tubule. Metallothionein, β2-microglobulin, α1-microglobulin, lipocalin-2, albumin and transferrin were investigated, both as apo- and Cd2+-protein complexes, in a rat proximal tubule cell line (WKPT-0293 Cl.2) expressing megalin:cubilin at low passage, but is lost at high passage. Uptake was determined by fluorescence microscopy and toxicity by MTT cell viability assay. Apo-proteins in low and high passage cells as well as Cd2+-protein complexes in megalin:cubilin deficient high passage cells did not affect cell viability. The data prove Cd2+-metallothionein is not toxic, even at >100-fold physiological metallothionein concentrations in the primary filtrate. Rather, Cd2+-β2-microglobulin, Cd2+-albumin and Cd2+-lipocalin-2 at concentrations present in the primary filtrate are taken up by low passage proximal tubule cells and cause toxicity. They are therefore likely candidates of Cd2+-protein complexes damaging the proximal tubule via megalin:cubilin at concentrations found in the ultrafiltrate.
Team sport expertise shows superior stimulus-driven visual attention and motor inhibition

Previous studies on athletes’ cognitive functions have reported superior performance on tasks measuring attention and sensorimotor abilities. However, how types of sports training shapes cognitive profile remains to be further explored. In this study, we recruited elite athletes specialized in badminton (N = 35, female = 12) and volleyball (N = 29, female = 13), as well as healthy adult controls (N = 27, female = 17) who had not receive any regular sports training. All participants completed cognitive assessments on spatial attention, sensory memory, cognitive flexibility, motor inhibition, and the attention networks. The results showed that athletes generally showed superior performance on selective cognitive domains compared to healthy controls. Specifically, compared to the healthy control, volleyball players showed superior on iconic memory, inhibitory control of action, and attentional alerting, whereas badminton players showed advantages on iconic memory and basic processing speed. Overall, volleyball players outperformed badminton players on those tasks require stimulus-driven visual attention and motor inhibition, likely due to different training modalities and characteristics of specialty that involves even more complex cognitive processes. To conclude, our findings suggest cognitive plasticity may drive by sports training in team/individual sports expertise, manifesting cognitive profile in sport expertise with distinct training modalities.
Protein melting temperature cannot fully assess whether protein folding free energy underlies the universal abundance-evolutionary rate correlation seen in proteins.

The protein misfolding avoidance hypothesis (MAH) explains the universal negative correlation between protein abundance and sequence evolutionary rate across the proteome by identifying protein folding free energy (ΔG) as the confounding variable. Abundant proteins resist toxic misfolding events by being more stable, and more stable proteins evolve slower because their mutations are more destabilizing. Direct supporting evidence consists only of computer simulations. A study taking advantage of a recent experimental breakthrough in measuring protein stability proteome-wide through melting temperature (Tm) (Leuenberger et al. 2017), found weak MAH support for the Escherichia coli proteome, and no support for the Saccharomyces cerevisiae, Homo sapiens and Thermus thermophilus proteomes (Plata and Vitkup 2017). I find that the nontrivial relationship between Tm and ΔG and inaccuracy in Tm measurements by Leuenberger et al. 2017 can be responsible for not observing strong positive abundance-Tm and strong negative Tm-evolutionary rate correlations. SUPPLEMENTARY INFORMATION Supplementary data are available online.
A comparison of all-cause and cause-specific mortality by household socioeconomic status across seven INDEPTH network health and demographic surveillance systems in sub-Saharan Africa

ABSTRACT Background: Understanding socioeconomic disparities in all-cause and cause-specific mortality can help inform prevention and treatment strategies. Objectives: To quantify cause-specific mortality rates by socioeconomic status across seven health and demographic surveillance systems (HDSS) in five countries (Ethiopia, Kenya, Malawi, Mozambique, and Nigeria) in the INDEPTH Network in sub-Saharan Africa. Methods: We linked demographic residence data with household survey data containing living standards and education information we used to create a poverty index. Person-years lived and deaths between 2003 and 2016 (periods varied by HDSS) were stratified in each HDSS by age, sex, year, and number of deprivations on the poverty index (0–8). Causes of death were assigned to each death using the InterVA-4 model based on responses to verbal autopsy questionnaires. We estimated rate ratios between socioeconomic groups (2–4 and 5–8 deprivations on our poverty index compared to 0–2 deprivations) for specific causes of death and calculated life expectancy for the deprivation groups. Results: Our pooled data contained almost 3.5 million person-years of observation and 25,038 deaths. All-cause mortality rates were higher among people in households with 5–8 deprivations on our poverty index compared to 0–2 deprivations, controlling for age, sex, and year (rate ratios ranged 1.42 to 2.06 across HDSS sites). The poorest group had consistently higher death rates in communicable, maternal, neonatal, and nutritional conditions (rate ratios ranged 1.34–4.05) and for non-communicable diseases in several sites (1.14–1.93). The disparities in mortality between 5–8 deprivation groups and 0–2 deprivation groups led to lower life expectancy in the higher-deprivation groups by six years in all sites and more than 10 years in five sites. Conclusions: We show large disparities in mortality on the basis of socioeconomic status across seven HDSS in sub-Saharan Africa due to disparities in communicable disease mortality and from non-communicable diseases in some sites. Life expectancy gaps between socioeconomic groups within sites were similar to the gaps between high-income and lower-middle-income countries. Prevention and treatment efforts can benefit from understanding subpopulations facing higher mortality from specific conditions.
Non-invasive cardiac mapping for non-response in cardiac resynchronization therapy

Abstract Cardiac resynchronization therapy (CRT) is an effective intervention in selected patients with moderate-to-severe heart failure with reduced ejection fraction and abnormal left ventricular activation time. The non-response rate of approximately 30% has remained nearly unchanged since this therapy was introduced 25 years ago. While intracardiac mapping is widely used for diagnosis and guidance of therapy in patients with tachyarrhythmia, its application in characterization of the electrical substrate to elucidate the mechanisms involved in CRT response remain anecdotal. In the present review, we describe the traditional determinants of CRT response before presenting novel non-invasive techniques used for CRT optimization. We discuss efforts to identify the target electrical substrate to guide the deployment of pacing electrodes during the operative procedure. Non-invasive body surface mapping technologies such as ECG imaging or ECG belt enables prediction of acute and chronic CRT response. While electrical dyssynchrony parameters provide high predictive accuracy for CRT response when obtained during intrinsic conduction, their predictive value is less when acquired during CRT or LV-pacing. Key messages Classic predictors of CRT response are female gender, NYHA class ≤ III, left ventricular ejection fraction ≥25%, QRS duration ≥150 ms and estimated glomerular filtration rate ≥60 mL/min. ECG-imaging is a comprehensive non-invasive mapping system which allows to express the amount of electrical asynchrony of a CRT candidate. Non-invasive body surface mapping technologies enables excellent prediction of acute and chronic CRT response before implantation. When performed during CRT or LV-pacing, the added value of these mapping systems remains unclear.
Association between Weather Types based on the Spatial Synoptic Classification and All-Cause Mortality in Sweden, 1991–2014

Much is known about the adverse health impact of high and low temperatures. The Spatial Synoptic Classification is a useful tool for assessing weather effects on health because it considers the combined effect of meteorological factors rather than temperature only. The aim of this study was to assess the association between oppressive weather types and daily total mortality in Sweden. Time-series Poisson regression with distributed lags was used to assess the relationship between oppressive weather (Dry Polar, Dry Tropical, Moist Polar, and Moist Tropical) and daily deaths over 14 days in the extended summer (May to September), and 28 days during the extended winter (November to March), from 1991 to 2014. Days not classified as oppressive weather served as the reference category. We computed relative risks with 95% confidence intervals, adjusting for trends and seasonality. Results of the southern (Skåne and Stockholm) and northern (Jämtland and Västerbotten) locations were pooled using meta-analysis for regional-level estimates. Analyses were performed using the dlnm and mvmeta packages in R. During summer, in the South, the Moist Tropical and Dry Tropical weather types increased the mortality at lag 0 through lag 3 and lag 6, respectively. Moist Polar weather was associated with mortality at longer lags. In the North, Dry Tropical weather increased the mortality at shorter lags. During winter, in the South, Dry Polar and Moist Polar weather increased mortality from lag 6 to lag 10 and from lag 19 to lag 26, respectively. No effect of oppressive weather was found in the North. The effect of oppressive weather types in Sweden varies across seasons and regions. In the North, a small study sample reduces precision of estimates, while in the South, the effect of oppressive weather types is more evident in both seasons.
Absence of expression of the Wiskott-Aldrich syndrome protein in peripheral blood cells of Wiskott-Aldrich syndrome patients.

Wiskott-Aldrich syndrome (WAS) is an X-linked primary immunodeficiency that is usually associated with thrombocytopenia and eczema. The very variable phenotype of WAS results from defects in the WAS protein (WASP), the function of which is not well understood. In many cases causative mutations have now been identified in the WAS gene. Attempts have been made to correlate the nature of the mutations with the severity of the disease. In this study we investigated mutations in 13 patients with WAS and analyzed the expression of WASP in patient blood samples by immunoblot analysis. We found that despite extensive variation in the nature of the mutations in patients with severe WAS symptoms, none express the protein. However, in 1 patient with a mild clinical phenotype WASP expression was detected. Such an analysis could be used as an initial screening procedure for the diagnosis of WAS prior to genotypic analysis.