Miracle-dz/UniFAR-FTUs huggingface dataset:
Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction.

Bacteriophage M13 procoat protein is synthesized on free polysomes prior to its assembly into the inner membrane of Escherichia coli. As an initial step of the membrane insertion pathway, the precursor protein interacts with the cytoplasmic face of the inner membrane. We have used oligonucleotide‐directed mutagenesis to study the regions of the procoat protein involved in membrane binding. We find that there is an absolute requirement for positively charged amino acids at both ends of the protein. Replacing these with negatively charged residues resulted in an accumulation of the precursor in the cytoplasm. We propose that the positively charged amino acids are directly involved in membrane binding, possibly directly to the negatively charged phospholipid head groups. This was tested in vitro with artificial liposomes. Whereas wild‐type procoat interacted with these liposomes, we found that procoat mutants with negatively charged amino acids at both ends did not bind. Therefore, we conclude that newly synthesized M13 procoat protein binds electrostatically to the negatively charged inner membrane of E. coli.
PENERAPAN METODE UTAUT UNTUK MEMPREDIKSI BEHAVIORAL INTENTIONS USER DALAM MENGGUNAKAN APLIKASI ZABBIX

Use of information technology is essential for the company, because it can increase the effectiveness and efficiency of the company's performance. But the application of information technology is not always successful. One of the critical success factors of the application of information technology is the attitude of the users who use the technology. UTAUT also used by some researchers to look at the intentions and behavior of users of information technology in several areas of the company. Therefore, this study was developed by adopting the model to see the intentions of the user UTAUT Zabbix Application in PT Media Nusantara Citra Tbk. Four constructs of UTAUT used as determinants that influence the behavioral intentions (behavioral intentions user), namely performance expectancy, effort expectancy, social influence and facilitating conditions. Data in the study were tested by means of SEM (Structural Equation Model). SEM is a statistical analysis tool used to complete multilevel research model simultaneously. SEM can be used to solve equations with variables that make up a path. Data obtained through 50 questionnaires distributed to PT Media Nusantara Citra Tbk and in particular the IT Infrastructure Division. The analysis showed that behavioral intentions are significantly influenced by the user, effort expectancy and facilitating conditions, while the performance expectancy and social factors influence found no significant effect.
Post-Vietnam Dioxin Exposure in Agent Orange-Contaminated C-123 Aircraft.

Foreword:Many readers are familiar with the extensive herbicide spraying operations to clear tropical foliage during the Vietnam War in order to prevent enemy forces from using greenery as cover. But it may not be apparent to many readers that some of the same C-123 aircraft used for spraying were shipped back to the Unites States and used by Air Force reserve units for training purposes. Some of those reservists believed that they were exposed to dioxin, a toxic contaminant in the herbicides, whose presence was established by a small number of surface samples from these planes. The IOM was asked to determine whether the residual level of dioxin in these planes could plausibly have been high enough to result in adverse health outcomes. Understanding the kinetics of dioxin degradation and movement in a closed space was critical to this assessment. The study committee was able to utilize a small amount of available data to reach its logical conclusions. —Frederick Erdtmann, MD, MPH, Director, Board on the Health of Select Populations, IOM
Service Quality Measurement Model of E-Banking Website: Validity and Reliability Test of the Research Instrument

This study is a preliminary study in order to find the right research instrument, to develop service quality measurement model for eBanking website in Indonesia using Webqual. The analysis techniques use in this study is the validity test with KMO and Bartlett’s test, and reliability test with Cronbach Alpha. Respondents in this study were clients of private banks National. The initial phase of this study that the respondents where selected from a Faculty of Computer Science and Information Technology majors in Banking Information Systems, Laboratory Assistant and lecturer in Gunadarma University. The limitation of this study was the number of respondents who meet the criteria for bank customers who use e-banking facilities. The first phase of deployment questionnaire results showed that the personality variable was not reliable, and there is one question that is not valid. The overall result shows that the research instrument is reliable and valid, so it can be used for the next research steps.
Innovators intent: role of IT in facilitating innovative knowledge practices in social enterprises

With this paper we want to explore further the innovators intent, where social enterprises use imaginative ways to take advantage of information technology to create, share and manage the knowledge pool of their small enterprise. We draw on several perspectives on how information processing needs are addressed, as well as the manner in which IT enables and facilitates sense-making. Studies exploring the role of IT in organisations abound, however our focus is not large organisations but small social enterprises (SEs) and how they use IT to further their business objectives. Hence there is still a lack of understanding on how IT can support the management of knowledge within the context of SEs dealing with different contextual settings influenced by: constant tensions between social and economic objectives, more focus on sustainability than competiveness, limited resources, and high levels of democratic participation. All these conditions manifest themselves in SEs, aiming to tackle social problems, improve communities, people’s quality of life, and environment. To obtain a conceptual and empirical understanding of how IT can facilitate acquisition, conversion and application of knowledge in SEs, we conducted a qualitative study with 21 interviews to owners, senior members and founders of SEs in the UK, underpinned by findings from a quantitative survey with 432 responses. We found how IT was supporting informal practices of knowledge management in SEs, more the recovery and storage of necessary information in SEs, and less the collaborative work and communication among enterprise members. However, it was established that SEs were using different technologies, such as, cloud solutions and web 2.0 tools to manage informally their knowledge. The possible impediments for SEs to support themselves more on IT solutions can be linked mainly to economic and human constraints. These findings elucidate new perspectives of how small and hybrid organisations are supporting their operations using IT and the crucial role of cloud and web 2.0 tools in facilitating informal knowledge management practices.
Limits of the "algebraic" specification of abstract data types

This paper deals with the feasibility of the algebraic specification technique for abstract data types, which consists of a declaration of the operations of a data type and a finite set of "axioms" for the operations. We discuss some inherent difficulties arising from this technique and present a simple example of an abstract type for which the method cannot be applied.
Effects of butenafine hydrochloride, a new benzylamine derivative, on experimental tinea pedis in guinea pigs

Butenafine is a new antifungal benzylamine. The efficacy of butenafine was investigated in an experimental tinea pedis model in guinea pigs, which is pathologically similar to natural infections in humans. Butenafine (0.1 ml) in 0.2 to 1.0% solutions was applied to the site of infection. Treatment was started on day 10 postinfection and was continued for 20 days. Butenafine applied once daily exhibited excellent dose-related therapeutic efficacy. The efficacy of butenafine was significantly superior to those tolnaftate, clotrimazole, and bifonazole.
A three-level learner's model for the needs of an integrated environment for individual planned teaching

The learner's knowledge, skills, psychological characteristics, and preferred style of learning have been used for individualization of teaching in different generations of computer-based systems. A survey indicates that the problem of the learner modeling in the intelligent distributed teaching and learning environments is still opened. In the present paper a three-level learner's model for the needs of an integrated environment for individual planned teaching is discussed.
An Endothelial Cell-Specific Requirement for the UL133-UL138 Locus of Human Cytomegalovirus for Efficient Virus Maturation

ABSTRACT Human cytomegalovirus (HCMV) infects a variety of cell types in humans, resulting in a varied pathogenesis in the immunocompromised host. Endothelial cells (ECs) are considered an important target of HCMV infection that may contribute to viral pathogenesis. Although the viral determinants important for entry into ECs are well defined, the molecular determinants regulating postentry tropism in ECs are not known. We previously identified the UL133-UL138 locus encoded within the clinical strain-specific ULb′ region of the HCMV genome as important for the latent infection in CD34+ hematopoietic progenitor cells (HPCs). Interestingly, this locus, while dispensable for replication in fibroblasts, was required for efficient replication in ECs infected with the TB40E or fusion-inducing factor X (FIX) HCMV strains. ECs infected with a virus lacking the entire locus (UL133-UL138 NULL virus) complete the immediate-early and early phases of infection but are defective for infectious progeny virus production. ECs infected with UL133-UL138 NULL virus exhibited striking differences in the organization of intracellular membranes and in the assembly of mature virions relative to ECs infected with wild-type (WT) virus. In UL133-UL138 NULL virus-infected ECs, Golgi stacks were disrupted, and the viral assembly compartment characteristic of HCMV infection failed to form. Further, progeny virions in UL133-UL138 NULL virus-infected ECs inefficiently acquired the virion tegument and secondary envelope. These defects were specific to infection in ECs and not observed in fibroblasts infected with UL133-UL138 NULL virus, suggesting an EC-specific requirement for the UL133-UL138 locus for late stages of replication. To our knowledge, the UL133-UL138 locus represents the first cell-type-dependent, postentry tropism determinant required for viral maturation.
Evaluation of a Group Cue-Exposure Treatment for Opiate Addicts

Twenty-four detoxified opiate addicts were randomized to an experimental group and a control group to evaluate efficacy of a group cue-exposure treatment to reduce or extinguish classically conditioned responses to drug-related stimuli. Assessment included psychophysiological responses (skin temperature, skin conductance level—SCL—, and heart rate) to a videotape and subjective measures (subjective craving, positive and negative affect) before and after the videotape. The experimental group received a group cue-exposure program to drug-related stimuli that comprised twelve treatment sessions administered three times weekly. The treatment program significantly reduced conditioned responses to drug-related stimuli, as measured by SCL and positive affect. Veinticuatro adictos a los opiáceos, ya desintoxicados, fueron asignados de forma aleatoria a un grupo experimental y un grupo control para evaluar la eficacia de un tratamiento de exposición para reducir o extinguir las respuestas condicionadas a los estímulos asociados con la droga. La evaluación incluyó las respuestas psicofisiológicas (temperatura periférica, nivel de conductancia dermoeléctrica y frecuencia cardiaca) emitidas ante la presentación de un video, así como medidas subjetivas (grado de “deseo” subjetivo, afecto positivo y negativo) antes y después de la presentación del video. El grupo experimental recibió un programa de exposición en grupo a estímulos relacionados con la droga, consistente en doce sesiones de tratamiento con una periodicidad de tres veces por semana. El programa de tratamiento redujo de forma significativa las respuestas condicionadas a los estímulos relacionados con la droga, de acuerdo con las medidas de nivel de conductancia electrotérmica y afecto positivo.
The Effects of Audit-Firm Monopolies within Local Audit Markets

This study identifies auditors who have 100% market shares in a city-industry audit market (hereinafter, monopolist auditors) and examines their pricing strategy. I document that monopolist auditors charge lower fees than do industry specialist auditors. This result is consistent with a monopolists’ pricing strategy (i.e., limit pricing) to deter new entrants but contrasts with regulators’ concerns about monopoly pricing. I further find that monopolist auditors more often fail to detect misstatements than do industry specialist auditors. This is consistent with regulators’ concerns about market-dominating auditors’ complacency. In cross-sectional tests, limit pricing is predominantly evident in homogenous operation industries where more profits are at stake. On the other hand, limit pricing is moderated when clients’ proprietary costs are high, which lowers auditor switching risk. In addition, I find that monopolist auditors’ audit failures are more pronounced when the current market competition within a city is low. These findings suggest that auditors exhibit distinctive incentives when they lack the closest competitor.
A homozygous ZMPSTE24 null mutation in combination with a heterozygous mutation in the LMNA gene causes Hutchinson‐Gilford progeria syndrome (HGPS): insights into the pathophysiology of HGPS

Hutchinson‐Gilford progeria syndrome (HGPS) is a rare premature aging disorder normally caused by a spontaneous heterozygous mutation in the LMNA gene that codes for the nuclear lamina protein lamin A. Several enzymes are involved in the processing of its precursor, prelamin A, to the mature lamin A. A functional knockout of one of the enzymes involved in prelamin A processing, the zinc metalloprotease ZMPSTE24, causes an even more severe disorder with early neonatal death described as restrictive dermatopathy (RD). This work describes a HGPS patient with a combined defect of a homozygous loss‐of‐function mutation in the ZMPSTE24 gene and a heterozygous mutation in the LMNA gene that results in a C‐terminal elongation of the final lamin A. Whereas the loss of function mutation of ZMPSTE24 normally results in lethal RD, the truncation of LMNA seems to be a salvage alteration alleviating the clinical picture to the HGPS phenotype. The mutations of our patient indicate that farnesylated prelamin A is the deleterious agent leading to the HGPS phenotype, which gives further insights into the pathophysiology of the disorder. Hum Mutat 27(6), 524–531, 2006. © 2006 Wiley‐Liss, Inc.
Preference of South African consumers for the eating quality and appearance of apples

The attributes that drive South African consumer preference for apple eating quality and appearance were investigated in this study. Black, white and Indian consumers of two age groups (18-25 or 26+) and different socio-economic background participated in consumer studies conducted in Pretoria, Gauteng province and Durban, Kwa-Zulu Natal province. Nine different commercial apple cultivars were used to attain variation in eating quality and appearance attributes. Descriptive sensory analysis was carried out on all nine cultivars using a trained panel. Consumer preference for eating quality and appearance of all nine cultivars was assessed on a 9-point hedonic scale. Principal component analysis (PCA) was used to project the outcome of descriptive sensory analysis onto consumers’ preference dimension. Region, ethnic and age groups as well as gender, interacted for consumer preference of eating quality. Black consumers from both Gauteng and Kwa-Zulu Natal and from both age groups (18-25 and 26+), as well as white and Indian consumers from the older age group (26+) had a high preference for sweet taste. White and Indian consumers from the young age group (1825) preferred cultivars that were crisp and firm. The male consumers’ eating quality preference associated positively with sweetness. Age, as well as region, ethnic group and gender interacted for consumer preference of appearance. The older (26+) consumers disliked the appearance of ‘Granny Smith’, ‘Braeburn’ and also, to a lesser extent, ‘Fuji’. Younger (18-25) consumers disliked the appearance of ‘Braeburn’ and ‘Fuji’ but preferred the appearance of ‘Granny Smith’. Black consumers generally preferred the appearance of ‘Golden Delicious’. Since ‘Golden Delicious’ associated with sweet taste, black consumers’ appearance preference associated with their eating quality preference. White and Indian consumers preferred the appearance of ‘Granny Smith’ which closely associated with sour taste and also with their eating quality preference. Our results indicate that specific apple cultivars could be targeted to specific ethnic groups to increase sales.
The Impact of Financial Development on Foreign Direct Investment in Nigeria

Abstract The study examined the relationship between foreign direct investment and financial development in Nigeria for the period 1980 to 2015. Using the vector error correction model (VECM) technique, the study observed a negative relationship existed between financial market development and foreign direct investment in the long run while in the short run, a positive relationship existed between financial market development and foreign direct investment in Nigeria over the estimated period of 1980-2015. Therefore, the study recommended further development of the Nigerian financial market by the monetary authority given its positive influence on the inflows of foreign direct investment in the short run. There is also the need for more financial reform of the Nigerian financial system in order to bring more foreign direct investment into the country both in the long and short runs. Keywords Financial development, Foreign Direct Investment, VECM, Nigeria
Calcium Oscillations and Exocytosis in Pituitary Gonadotropes a

Our laboratory has been interested in the intracellular signaling mechanisms leading to exocytosis in anterior pituitary cells. The anterior pituitary contains five secretory cell types, each under specific control of releasing hormones and neurotransmitters released by hypothalamic neurons. Surprisingly, the intracellular signals used to turn on different anterior pituitary cells are not all the same. Indeed, the releasing-hormone receptors of the different cell types couple to different G proteins and initiate different second-messenger cascades. For example, gonadotropin-releasing hormone (GnRH) receptors couple to a pertussis toxin-insensitive G protein to activate phospholipase C in gonadotropes, and growth-hormone-releasing hormone (GHRH) receptors couple to G, to activate adenylyl cyclase in somatotropes. How do such different stimuli lead to the common end result, namely, exocytosis of hormones? This report describes a picture of GnRH-mediated signaling in gonadotropes (FIGURE 1) derived from our work and that of others. The approaches we have used combine patch-clamp methods' and fluorescent Ca2+ indicators,2 as we have described b e f ~ r e . ~ ~ In brief, anterior pituitaries of (56)-week-old male rats are dissociated to yield individual cells and are plated for culture. Gonadotropes are positively identified by the reverse-hemolytic-plaque assay and are studied by whole-cell clamp methods to record their ionic currents and membrane capacitance. The intracellular free Caz+ is measured in the single cell by microphotometry with the dye indo-1 loaded into the cell via the recording pipette.
A New Paradigm for Systematic Literature Reviews in Supply Chain Management

While systematic literature reviews (SLRs) have contributed substantially to developing knowledge in fields such as medicine, they have made limited contributions to developing knowledge in the supply chain management domain. This is due to the ontological and epistemological idiosyncrasies of research in supply chain management, which need to be accounted for when retrieving, selecting, and synthesizing studies in an SLR. Therefore, we propose a new paradigm for SLRs in the supply chain domain that is based on both best practice and the unique attributes of doing supply chain management research. This approach involves exploring existing studies with attention to theoretical boundaries, units of analysis, sources of data, study contexts, and definitions and the operationalization of constructs, as well as research methods, with the goal of refining or revising existing theory. This new paradigm will push supply chain management research to the frontier of current methodological standards and build a foundation for improving the contribution of future SLRs in the supply chain and adjacent management disciplines.
The Role of Beliefs on Learning About Homosexuality in a College Course

The present research investigated how personal beliefs about homosexuality influence learning in a college course. We tested students in introductory psychology over material on the science of homosexuality by Simon LeVay (2010). All students reported information about their typical academic habits and the extent to which homosexuality was consistent with their beliefs and values. The results showed that students’ personal beliefs were related to academic behaviors (e.g., reading assignments, skipping class) and retention of the course material. The results also showed that students’ recall of course material six weeks later was predicted by the extent to which they reported studying information that is inconsistent with their beliefs for an exam and then forgetting it. Students who reported the material to be inconsistent with their beliefs engaged in selective forgetting of the material on homosexuality. The results provide evidence that personal beliefs can reduce the retention of belief-inconsistent information in a college course.
Intention to Purchase Counterfeit Luxury Products: A Comparative Study Between Pakistani and the UK Consumers

ABSTRACT This study compares Pakistani and the UK consumers' purchase intentions toward counterfeit luxury products by focusing on the relationships between the following four factors: perceived quality, status consumption, low price, and ethics. Two samples of students from the UK (114) and Pakistan (137) were surveyed for comparative purposes. Data were analyzed by using AMOS and SPSS. The results show that while Pakistani consumers are satisfied with the perceived quality of counterfeit products, the UK consumers are not. The status associated with counterfeit products and price of the products were identified as important factors for both populations. However, Pakistani consumers exhibit less ethical behavior compare to the UK consumers. Thus, the study provides evidence that intention for purchasing counterfeit luxury products differs between the two populations and thus companies may have to differentiate their strategies in tackling the problem in developing and developed countries. Selecting a single product category and a single range of products may restrict generalizability.