Miracle-dz/UniFAR-FTUs huggingface dataset:
Activity-dependent long-term potentiation of electrical synapses in the mammalian thalamus

Activity-dependent changes of synapse strength have been extensively characterized at chemical synapses, but the relationship between physiological forms of activity and strength at electrical synapses remains poorly understood. For mammalian electrical synapses composed of hexomers of connexin36, physiological forms of neuronal activity in coupled pairs has thus far have only been linked to long-term depression; activity that results in strengthening of electrical synapses has not yet been identified. The thalamic reticular nucleus (TRN), a central brain area primarily connected by gap junctional (electrical) synapses, regulates cortical attention to the sensory surround. Bidirectional plasticity of electrical synapses may be a key mechanism underlying these processes in both healthy and diseased states. Here we show in electrically coupled TRN pairs that tonic spiking in one neuron results in long-term potentiation of electrical synapses between coupled pairs of TRN neurons. Potentiation is expressed asymmetrically, indicating that regulation of connectivity depends on the direction of use. Further, potentiation depends on calcium flux, and we thus propose a calcium-based activity rule for bidirectional plasticity of electrical synapse strength. Because electrical synapses dominate intra-TRN connectivity, these synapses and their modifications are key regulators of thalamic attention circuitry. More broadly, bidirectional modifications of electrical synapses are likely to be a widespread and powerful principle for ongoing, dynamic reorganization of neuronal circuitry across the brain. Summary Long-term potentiation results from spiking in one cell of an electrically coupled pair. Asymmetry of synapses increases following unidirectional activity. We suggest a calcium-based rule for electrical synapse plasticity.
Therapeutic value of the genus Alpinia , Zingiberaceae Cristiane

Plants containing bioactive substances have increasingly become the object of research studies, particularly those plants with therapeutic value. Many species of the genus Alpinia provide a variety of medicinal properties, such as, Alpinia zerumbet (Pers.) Burtt et Smith and A. purpurata (Vieill) K. Schum, which have a significant presence in Brazil. These species have been commercialized in the food and cosmetic industries. However, their greatest importance arises from the medicinal properties of their essential oils containing flavonoids, terpenoids and kavalactones which have been used in folk medicine to treat, for example, arterial hypertension and inflammatory processes. In addition, such species are also used in multidisciplinary studies, including phytochemistry, ethnobotany and biology, indicating the key pharmacological role of this genus in everyday life. Therefore, this work aims to present a bibliographic review of the genus Alpinia and its significance in therapeutic applications.
Roles of Three Transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa Choline Uptake for Catabolism

ABSTRACT Pseudomonas aeruginosa uses the quaternary amine choline as a carbon source, osmoprotectant, and macromolecular precursor. The importance of choline in P. aeruginosa physiology is highlighted by the presence of multiple known and putative choline transporters encoded within its genome. This report describes the relative roles of three choline transporters, the ABC transporter CbcXWV and two symporters, BetT1 and BetT3, in P. aeruginosa growth on choline under osmotic conditions that are physiologically relevant to eukaryotic hosts. The increased lag phases exhibited by the ΔbetT1 and ΔbetT1 ΔbetT3 mutants relative to the wild type upon transfer to medium with choline as a sole carbon source suggested roles for BetT1 and BetT3 in cells newly exposed to choline. BetT3 and CbcXWV, but not BetT1, were sufficient to support growth on choline. betT1 and betT3 expression was regulated by the repressor BetI and choline, whereas cbcXWV expression was induced by the activator GbdR and glycine betaine. The data support a model in which, upon transfer to a choline-based medium, the glycine betaine derived from choline taken up by BetT1 and BetT3 promotes subsequent GbdR-mediated cbcXWV induction. Furthermore, growth data indicated that the relative contributions of each transporter varied under different conditions, as BetT1 and CbcXWV were the primary choline transporters under hypo-osmolar conditions whereas BetT3 was the major choline transporter under hyperosmolar conditions. This work represents the first systematic approach to unravel the mechanisms of choline uptake in P. aeruginosa, which has the most complex bacterial choline uptake systems characterized to date.
Evaluation of Heterotopic Ossification After Using Recombinant Human Bone Morphogenetic Protein–2 in Transforaminal Lumbar Interbody Fusion: A Computed Tomography Review of 996 Disc Levels

Study Design: Retrospective cohort study. Objectives: Reported incidences and complications of heterotopic ossification (HO) after using recombinant human bone morphogenetic protein–2 (rhBMP-2) in transforaminal lumbar interbody fusion (TLIF) have been inconclusive. This study was designed to evaluate both incidences of radiologic and symptomatic HO in a large series of TLIFs using rhBMP-2. Methods: A total of 996 disc levels in 927 consecutive TLIF patients were retrospectively evaluated at 6-month postoperative follow-up in a single surgical practice. Subjects were separated into the BMP group and the control group. Operative reports, pre- and postoperative medical records were reviewed. Computed tomography (CT) scans were analyzed and graded independently for ossification at each disc level of TLIF. Results: A total of 933 disc levels were in the BMP group, and 63 were in the control group. Six-month fusion rate of interbody was 92.5% in the BMP group, which was significantly higher in contrast to 71.4% in the control group (P < .001). The incidence of radiologic HO in the BMP group was 13.5%, which was significantly higher than 1.6% in the control group (P = .006). After controlling for basic demographics and comorbidities, the presence of radiologic HO was significantly associated with the use of rhBMP-2 (P = .026). However, only one case in the BMP group (0.11%) developed a symptomatic HO (mild-medium left buttock pain, treated nonsurgically) involving left foramen of L5-S1. Conclusions: rhBMP-2 can be safely used in TLIF with regard to HO. There was a low rate of radiologic HO and minimal symptomatic HO, with high fusion rates at 6 months postoperative.
Mineralogy of the clay fraction of alfisols in two slope curvatures: IV - spatial correlation with physical properties

Although the influence of clay mineralogy on soil physical properties has been widely studied, spatial relationships between these features in Alfisols have rarely been examined. The purpose of this work was to relate the clay minerals and physical properties of an Alfisol of sandstone origin in two slope curvatures. The crystallographic properties such as mean crystallite size (MCS) and width at half height (WHH) of hematite, goethite, kaolinite and gibbsite; contents of hematite and goethite; aluminium substitution (AS) and specific surface area (SSA) of hematite and goethite; the goethite/(goethite+hematite) and kaolinite/(kaolinite+gibbsite) ratios; and the citrate/bicarbonate/dithionite extractable Fe (Fed) were correlated with the soil physical properties through Pearson correlation coefficients and cross-semivariograms. The correlations found between aluminium substitution in goethite and the soil physical properties suggest that the degree of crystallinity of this mineral influences soil properties used as soil quality indicators. Thus, goethite with a high aluminium substitution resulted in large aggregate sizes and a high porosity, and also in a low bulk density and soil penetration resistance. The presence of highly crystalline gibbsite resulted in a high density and micropore content, as well as in smaller aggregates. Interpretation of the cross-semivariogram and classification of landscape compartments in terms of the spatial dependence pattern for the relief-dependent physical and mineralogical properties of the soil proved an effective supplementary method for assessing Pearson correlations between the soil physical and mineralogical properties.
Plant diversity changes in a nature reserve: a probabilistic sampling method for quantitative assessments

Species pool conservation is critical for the stability of ecosystem processes. However, climate and land use changes will likely affect biodiversity, and managers of protected areas are under increasing pressure to monitor native species diversity changes by approaches that are scientifically sound and comparable over time. Here we describe a plant diversity monitoring system in use since 2002 in the “Montagna di Torricchio” Nature Reserve (LTER_EU_IT_033), a Central Apennines representative area of 317 ha, most of which is under strict protection. The aim of this paper was to assess changes in plant species richness over time and to deduce the patterns of species assemblage. The monitoring system was based on a probabilistic sampling design representative of the different physiognomic vegetation types occurring in the Reserve. A total of 34 plots (10×10m) were sampled in 2002, 2003 and 2015, and their species presence/absence and relative coverage were estimated. Repeated measure ANOVA was used to test for plot-level and ecosystem-based changes in species richness along the study period. Temporal nestedness and temporal turnover metrics were used to assess patterns of species’ compositional changes. The results showed significantly different levels of species richness depending on the year, with the lowest value in 2003, probably linked to extreme drought events. Forest systems were comparatively stable, demonstrating the capacity to buffer interannual climate variability. Regarding compositional changes along the entire period (2002–2015), we found random patterns of both temporal nestedness and turnover, indicating stability in species composition. However, we also showed the contemporary occurrence of species loss and species replacement processes, considering the dry year 2003, a finding which should be further explored through fine-scale studies to unravel mechanisms of community assembly under drought. The use of a probabilistic sampling design representative of the different physiognomic vegetation types proved to be advantageous in monitoring the Nature Reserve vegetation and collecting reliable quantitative information. This data, in turn, provides the basis for improvements in management practices and proposed adaptation measures.
Self-configuration mechanisms for SDN deployment in Wireless Mesh Networks

Software-Defined Networking (SDN) is a framework conceived to make network infrastructures more programmable and more easily manageable. We explore the integration of the latter in Wireless Mesh Networks (WMNs) through the design of an opportunistic and self-configurable SDN solution. Opportunistic to be able to cope with the intermittent connectivity and network partitioning and merging scenarios caused by the dynamic nature of the wireless medium; and self-configurable to be able to redirect dynamically SDN-enabled Wireless Mesh Routers (WMRs) to the most suitable SDN controller. This paper proposes mechanisms allowing to make automatic an SDN-based WMN deployment. Our proposed solution enables (1) for each WMR to discover the reachable SDN controllers, then to select and connect by itself to the most suitable one; (2) the setting up of new SDN controller(s) among the WMRs in case of controllers unavailability. We validated our work using NEON, an SDN solution developed by CEA LIST that supports fast devices configuration and services deployment in dynamic and unconfigured infrastructures contexts.
Study on Honey Quality Evaluation and Detection of Adulteration by Analysis of Volatile Compounds

Abstract Fast evaluation of honey quality is a topical and significant problem of the food industry, bee keepers and consumers. In this work, 22 samples of commercially available honey aromas (with methyl and ethyl esters of phenylacetic acid predominated), 13 samples of authentic honey collected directly from bee keepers (characterised by high content of benzaldehyde, 2-phenylethanol, hotrienol and 2-phenylacetaldehyde) and 63 honeys purchased from an outdoor market were evaluated based on volatiles profiles determined through solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME-GC/MS) and then suspicious samples were identified. The results were statistically processed and compared with results of a sensory analysis. Six honeys, which differed significantly in volatiles profiles (outliers detected by Factor Analysis), selected volatile substance representation (furan-2-carbaldehyde, 1,4-dimethylpyrazole, benzaldehyde, 2-phenylacetaldehyde) and honey aroma intensity and pleasantness were subjected to targeted analyses (i.e. determination of 5-(hydroxymethyl)-2-furaldehyde, diastase activity, unauthorized additive presence). Four of these suspicious samples were found to have high content of 5-(hydroxymethyl)-2-furaldehyde (more than 40 mg/kg), three honeys had low values for diastase activity (less than 8) and three samples positive for triacetin addition. The fact that all these samples revealed a breach of least one of the selected quality parameters defined by the Codex Alimentarius standard proved the proposed methodology to be a useful tool for fast quality evaluation of honey.
Acylation-dependent Protein Export inLeishmania *

The surface of the protozoan parasiteLeishmania is unusual in that it consists predominantly of glycosylphosphatidylinositol-anchored glycoconjugates and proteins. Additionally, a family of hydrophilic acylated surface proteins (HASPs) has been localized to the extracellular face of the plasma membrane in infective parasite stages. These surface polypeptides lack a recognizable endoplasmic reticulum secretory signal sequence, transmembrane spanning domain, or glycosylphosphatidylinositol-anchor consensus sequence, indicating that novel mechanisms are involved in their transport and localization. Here, we show that the N-terminal domain of HASPB contains primary structural information that directs both N-myristoylation and palmitoylation and is essential for correct localization of the protein to the plasma membrane. Furthermore, the N-terminal 18 amino acids of HASPB, encoding the dual acylation site, are sufficient to target the heterologousAequorea victoria green fluorescent protein to the cell surface of Leishmania. Mutagenesis of the predicted acylated residues confirms that modification by both myristate and palmitate is required for correct trafficking. These data suggest that HASPB is a representative of a novel class of proteins whose translocation onto the surface of eukaryotic cells is dependent upon a “non-classical” pathway involvingN-myristoylation/palmitoylation. Significantly, HASPB is also translocated on to the extracellular face of the plasma membrane of transfected mammalian cells, indicating that the export signal for HASPB is recognized by a higher eukaryotic export mechanism.
The Buffering Effect of Religiosity on the Relationship Between Intolerance of Uncertainty and Depressive Symptoms

The purpose of the present study was to examine religiosity as a moderator of the relationship between intolerance of uncertainty (IU) and depressive symptoms in a large sample of general population adults recruited via the Internet (N = 566). Hierarchical regression revealed a significant interaction between IU and religiosity predicting depressive symptoms. Simple slopes analysis was used to further examine the significant interaction. Results of the simple slopes analysis indicated that the positive association between IU and depression scores was significantly stronger at lower, versus higher, levels of religiosity. This finding supports the hypothesis that religiosity may serve as a self-regulatory mechanism, furnishing religious practitioners with prescriptive norms that provide structure and order, thus reducing the impact of IU on depressive symptoms. These findings will be discussed in relation to two specific facets of IU (Inhibitory and Prospective IU).
The Coming Generational Storm

This paper extends the Auerbach-Kotlikoff life-cycle simulation model by incorporating demographic change, including lifespan extension, and multiple earnings groups within each cohort. The model is used to study the U.S. demographic transition. To ensure a realistic pattern of fertility by age, the model assumes that each agent gives birth to fractions of children during his child-bearing ages. The new model can also initiate transitions from arbitary initial conditions. The baseline simulation, which assumes a continuation of social security pay-as-you-go finance, exhibits sharp increases over time in the payroll tax rate. This increase siphons away so much saving that capital shallowing, rather than capital deepening, occurs over time. Hence, general equilibrium feedback effects exacerbate, rather than mitigate, the problems arising from population aging. The paper also simulates a range of policy reforms, including privatizing social security with different finance mechanisms. The simulations indicate that, compared with a continuation of current policy, privating social security would generate very major long-run welfare gains, particularly for the poorest members of society.
Preliminary data on the distribution, morphology and genetics of white-clawed crayfish and on their ectosymbionts in Lunigiana (Tuscany, Italy)

The white-clawed crayfish is a complex of species (Austropotamobius pallipes plus A. italicus , Crustacea) pivotal to riverine environments. Regrettably, it is endangered in many European countries due to a recent massive decline. We carried out a fine-scale survey on white-clawed crayfish distribution, morphology and genetics in an under-investigated region of central Italy (Lunigiana, Tuscany) to improve local knowledge and aid conservation management of the species. The torrential fauna of Lunigiana is scarcely known and habitat alterations due to the anthropic exploitation of the territory represent a potential threat to its persistence. We investigated crayfish occurrence in eight streams of the Magra River Basin. We performed nocturnal and diurnal transects, took morphometric measurements, collected samples for genetic analysis and checked for the occurrence of ectosymbionts. Crayfish were disclosed in two streams (Civasola, Verdesina). Morphometric and phylogenetic analysis (mtDNA COI gene Bayesian tree reconstruction) concurrently identified both populations as Austropotamobius italicus carinthiacus . In the Verdesina stream, crayfish were heavily infested by branchiobdellidans (Annelida). Verdesina crayfish were also significantly smaller than those found in the Civasola stream, where branchiobdellidans were absent. Hence, we hypothesized that such difference in size might be related to the high density of ectosymbionts. In the light of habitat features and of data herein provided, we propose that both Civasola and Verdesina streams should be considered as “natural ark sites” for A. italicus , with the Magra River Basin representing a regional stronghold for the conservation of the species.
Impact of Corporate Governance on Job Satisfaction of employees in Uganda

Empirical research in corporate governance have got much attention due to deficiencies in organizations. This research study has tried to explore the predictive relationship between corporate governance and employee job satisfaction, and dimensions of corporate governance (i.e., internal control, corporate structure and code of governance) and employee job satisfaction. The correlational research design was utilized to investigate the problem with a convenient sample of 196 respondents. Pearson Product-Moment correlation test, Standard regression, and hierarchical regression tests were used to test the hypotheses in this study. The result showed that corporate governance significantly and positively predicted employee job satisfaction. The three dimensions of corporate governance (i.e., corporate structure, code of governance and internal control) significantly and positively predicted employee job satisfaction with corporate structure accounting for more variance in employee job satisfaction than the other two dimensions. The implications of the findings with respect to theory have been discussed
The Political Entrepreneur and His Territory

This article explains the posture of the entrepreneurs to profit opportunities by the cognitive space in which they move. The identification of opportunities depends on local knowledge of times and places had by entrepreneurs. Each entrepreneur identifies different opportunities because each entrepreneur has a local knowledge which is specific for him. This dispersion of knowledge is explained by the fact that the entrepreneurs dot no move in same cognitive spaces. They are captive of what they know and what they know depends on the cognitive space which is theirs. The experiment of the businesses results in identifying market opportunities whereas the knowledge of the procedures of group decision and administrative procedures predisposes in the search of political profits. This demonstration is the subject of the second section of the article. It is preceded by a first section which develops the theoretical framework in which the question of the identification of the political profits arises.
The Systemic Risk of Cross-Border Banking: Evidence from the Sudden Stop and Interbank Stress Contagion in East Asia

ABSTRACT This article investigates the systemic risk of cross-border banking in East Asia. Using the recursive bivariate probit model, we jointly test the probability of the sudden stop in international lending and its simultaneous effect on the host countries’ interbank markets. The empirical results suggest that the risk of a sudden stop is associated with global liquidity shock; host country productivity shock; and the common lender contagion effect. This facilitates the transmission of interbank stress from advanced economies to emerging markets. However, the tension is mitigated by the “flight-home effect” caused by domestic investors’ repatriation. The sudden stop is more likely to occur in countries with lower financial openness but higher financial risk. Lending flows to the banking sectors are more sensitive to shocks than the flows to the non-bank private sectors.
Reducing Complexity with Simplicity - Usability Methods for Industry 4.0

At RWTH Aachen University the research cluster “Production Technology for High-Wage Countries” engages in advancing the polylemma of production. In many cases engineers and physicists develop simulation tools, machine interfaces, and data exploration tools but lack essential training in HumanComputer Interaction. Without proper training the interaction of visual, cognitive and task complexity can lead to solutions that are valuable only to the developers themselves, but are not usable without extensive training. We show the most critical ergonomic factors for developing software in a scientific engineering setting that focuses on complex problems. We present an overview of usability methods as well as complexity reduction methods and their applicability in engineering software design. We present an exemplary study for the case of supply chain management, where the approaches were successfully integrated into a serious game not only serving as an investigatory tool but also as a training utility for supply chain managers.