Miracle-dz/UniFAR-FTUs huggingface dataset:
Medical decision analysis for first-line therapy of chronic myeloid leukemia

Abstract Several tyrosine kinase inhibitors (TKIs) are approved for the treatment of chronic myeloid leukemia (CML). Our goal was to develop a clinical decision-analytic model for evaluation of the long-term effectiveness of different therapy regimens. We developed a Markov cohort model with a lifelong time horizon for first-line treatment with imatinib, dasatinib or nilotinib. Seven strategies including combinations of TKIs, chemotherapy and stem cell transplant were evaluated. The model was parameterized using published trial data, the Austrian CML registry and practice patterns estimated by experts. Health outcomes evaluated were life-years (LYs) and quality-adjusted LYs (QALYs). Based on our decision analysis, dasatinib following nilotinib failure was the most effective treatment in terms of LYs (19.8 LYs) and QALYs (16.1 QALYs). Sensitivity analyses showed that the ranking of strategies was mostly influenced by the duration of first- and second-line therapies. Our results may support decision-making regarding the sequential application of TKIs.
Halothane and Isoflurane Decrease Calcium Sensitivity and Maximal Force in Human Skinned Cardiac Fibers

BackgroundReports of the direct effects of volatile anesthetics on cardiac myofibrils, studied in various mammalian species but not in humans, have conflicted. To determine whether volatile anesthetics directly affect cardiac contractile proteins in humans, we examined the effects of various equianesthetic doses of halothane (0.46, 0.83, and 1.23 mM, equivalent to 0.75, 1.50, and 2.25%, respectively) and isoflurane (0.63, 1.22, and 1.93 mM, equivalent to 1.15, 2.30, and 3.50%, respectively) on the Ca2+ sensitivity and maximal force in human skinned cardiac fibers. MethodsLeft ventricular muscle strips were obtained from seven patients undergoing cardiac surgery. Sarcolemma was disrupted with EGTA (ethylene glycol bis (β-aminoethyl ether)-N,N,N‘,N’-tetraacetic acid), and sarcoplasmic reticulum was destroyed with EGTA plus BRIJ 58 detergent. Ca2+ sensitivity was studied by observing the isometric tension developed by skinned fiber bundles challenged with solutions of increasing Ca2+ concentrations expressed in pCa (where pCa = -log10[Ca2+]). Maximal force was measured with a pCa 4.8 solution. ResultsBoth anesthetics shifted the pCa-tension curves toward higher Ca2+ concentrations and decreased pCa for half-maximal activation in a dose-dependent and reversible fashion (from 5.71 for control to 5.56 and 5.55 for 1 MAC halothane and isoflurane, respectively) without changing the slope of this relationship (Hill coefficient). No differences between agents were observed at equianesthetic concentrations. The two agents also decreased the maximal activated tension in a dose-dependent fashion (-27 and −28% vs. control for 2 MAC halothane and isoflurane, respectively). ConclusionsThe current study indicates that halothane and isoflurane decrease Ca2+ sensitivity and maximal force in human skinned cardiac fibers at 20°C. If these effects extend to higher temperatures, they may contribute to the negative inotropic effect of these agents.
A survey of antimicrobial susceptibility of clinical isolates of Enterococcus spp. from Irish hospitals.

Nosocomial enterococcal infections are increasing. In order to establish the species distribution and antibiotic resistance patterns of enterococci in clinical specimens from hospitalized patients, we undertook a survey of 23 Irish hospitals. One thousand and five viable enterococcal strains were studied. Nine different species of enterococci were identified, including Enterococcus faecalis (84%); Enterococcus faecium (9%); and Enterococcus hirae (3%). The most common sites of isolation were the urinary tract (66%), wound and soft tissues (23%) and blood stream (3%). Many of the isolates were multiply antibiotic resistant. Ampicillin resistance was detected in 16%. Neither beta-lactamase production, nor high level penicillin resistance was detected. High level gentamicin resistance was evident in 7% of isolates and varied among species, e.g. 4% E. faecalis, 24% E. faecium, and 34% E. hirae. A number of isolates (23%) were also highly resistant to streptomycin. No clinically significant glycopeptide resistance was detected. The species distribution and incidence of multiple resistance was geographically widespread. This emphasizes the need for detailed speciation and in-vitro susceptibility testing along with the evaluation of alternative combination chemotherapeutic regimens for the management of serious enterococcal infection.
Losartan increases renal blood flow during isoflurane anesthesia in sheep

Background: Inhaled anesthetics cause a transient reversible depression of renal function by direct renal effects or indirectly by changes in neurohumoral systems or cardiovascular performance. When the sympathetic nervous activity is decreased during anesthesia, other vasoactive systems like vasopressin (AVP) and particularly the renin angiotensin system (RAS) are of importance for blood pressure maintenance. Little is known about how the renal circulation is affected by angiotensin receptor blockade during isoflurane anesthesia.
Mutations in the principal neutralization determinant of human immunodeficiency virus type 1 affect syncytium formation, virus infectivity, growth kinetics, and neutralization

The principal neutralization determinant (PND) of human immunodeficiency virus type 1 envelope glycoprotein gp120 contains a conserved GPG sequence. The effects of a 29-amino-acid deletion of most of the PND, a 3-amino-acid deletion in the GPG sequence, and 16 single-amino-acid substitutions in the GPG sequence were determined in a transient expression assay. All mutant envelope glycoproteins were expressed at levels comparable to that of the wild-type envelope, and mutations in the GPG sequence did not affect processing to gp120 or, except for the 29-amino-acid deletion, binding to CD4. Of all of the mutants, only the GHG and GFG mutants induced formation of syncytia similar in size and number to those induced by the wild-type envelope. When the envelope expression level was increased 10-fold or more, several additional mutants (APG, GAG, GSG, GQG, GVG, and GPF) also induced syncytium formation. Transfection with infectious proviral molecular clones containing the GHG, GFG, APG, GAG, GSG, or GPF mutations induced production of viral particles; however, only the GPG, GHG, and GFG viruses produced active infections in CD4-bearing cells. Furthermore, whereas the wild-type virus was efficiently neutralized by PND polyclonal and monoclonal antibodies, the GHG- and GFG-containing viruses were not. These results show that mutations in the GPG sequence found within the PND do not affect envelope expression and do not significantly affect CD4 binding or production of viral particles but that they do affect the ability of the envelope to induce syncytia and those of the viral particles to infect CD4 cells and be neutralized by PND antibodies.
Repeated Administration of Synthetic Oligodeoxynucleotides Expressing CpG Motifs Provides Long-Term Protection against Bacterial Infection

ABSTRACT Synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate an innate immune response characterized by the production of polyreactive immunoglobulin M antibodies and immunomodulatory cytokines. This immune response has been shown to protect mice from challenge by Listeria monocytogenes andFrancisella tularensis for up to 2 weeks. By repeatedly administering CpG ODN two to four times/month, we found that this protection could be maintained indefinitely. Protection was associated with a significant increase in the number of spleen cells that could be triggered by subsequent pathogen exposure to secrete gamma interferon and interleukin-6 in vivo (P < 0.01). ODN-treated animals remained healthy and developed neither macroscopic nor microscopic evidence of tissue damage or inflammation. Thus, repeated administration of CpG ODN may provide a safe means of conferring long-term protection against infectious pathogens.
Wireless Sensor Networks for Industrial Applications: Practical Approach

Wireless Sensor Networks (WSN) provides information for data acquisition and data distribution. It is a network that consists of spatially distributed and automated wireless sensor nodes which are capable of monitoring several physical environmental fields such as air pressure, luminosity, vibration and temperature. This paper focuses on the design of a WSN utilising ZigBee protocol and Waspmote technologies; the purpose is to provide telemetry services for industrial applications which will be required to collect data about light bulb luminosity as well as its associated surroundings. At the end of the processes, WSN framework was designed showing initial results of bulb luminescence reading, temperature and accelerometer; the designed system has the capability of sending real-time data over a network platform utilising the ZigBee 802.15. 4 protocol with sensor nodes attached to the circuit board; this will provide radio frequency telemetric services, utilising a cost effective platform that has the benefits of overcoming the cumbersome measures presently in operation. Sensor networks methods of development overtime have suffered setbacks for lack of well-defined practical approach that will produce results patterned for industrial acceptance; this paper measures on this area using a model that is environmental and eco-friendly.
Nonlinear modal analysis of the bar in unilateral contact via analytical weak-solutions to the wave equation

Nonlinear modal analysis is commonly performed by detecting families of periodic solutions of nonlinear conservative and autonomous systems. For a unilaterally constrained bar, nonlinear modes were detected using numerical solvers. In the present work, the analytical solution is investigated instead. Results show that infinitely many periodic solutions with the same energy and period might co-exist.
Primary 23-gauge sutureless vitrectomy for rhegmatogenous retinal detachment

Aims: To report a prospective non-comparative consecutive interventional study on the safety and efficacy of 23-Gauge transconjunctival sutureless pars plana vitrectomy for primary rhegmatogenous retinal detachment (RRD). Materials and Methods: Fifty eyes of 50 consecutive patients were recruited between June 2007 and January 2008. All surgeries were performed using the one-step 23-Gauge system with angled incisions. The surgical protocol consisted of a minimum of eight clinical visits: baseline, 1 day, 1 week, 1-, 3- and 6- months after the initial surgery. The endpoints were anatomical, functional results and complications arising from the surgery. Results: Anatomical success was achieved in 82% of cases (41 out of 50) with single surgery and rose to 98% (49 out of 50) with additional surgery. Mean visual acuity improved from logMAR 0.48(SD 0.36) to 0.26(SD 0.31), P < 0.001. Two cases with ocular hypotony, defined as an intraocular pressure ≤ 6mmHg, that were associated with a choroidal detachment were seen. Conclusions: Acceptable anatomical and functional success rates can be achieved with primary 23-Gauge transconjunctival sutureless vitrectomy for RRD. We found that the approach technique is different from conventional vitrectomy and the complications arising from post surgical hypotony and leakage from sclerotomies are potentially higher compared to 20-Gauge vitrectomy.
Long-Term Follow-up in Managing Anaplastic Astrocytoma by Multimodality Approach With Surgery Followed by Postoperative Radiotherapy and PCV-Chemotherapy: Phase II Trial

Overall survival and progression-free survival after 5 and 10 years of 31 patients with malignant glioma treated by a combination of surgery, postoperative radiotherapy, and chemotherapy with a PCV regimen (procarbazine, CCNU [lomustine] and vincristine) is described. Parameters were evaluated by age at diagnosis, gender, ethnic origin, pre- and postsurgery Karnofsky Performance Status (KPS) score, limit and amount of surgical resection, histopathologic type, number of chemotherapy courses, time between surgery and radiotherapy, response to combined therapy, and dosage and type of radiotherapy. Progression-free survival was 29% at 24 months and 22% at 60 and 120 months. Overall survival was 47%, 36%, and 36% after 24, 60, and 120 months, respectively. Favorable prognostic factors for survival in univariate analysis were pre- and postoperative KPS (≥70;p = 0.015;p = 0.0025, respectively), age of patients (<40;p = 0.01), number of chemotherapy cycles (≥6;p = 0.02), and radiation dose (≥60 Gy;p = 0.0015). The only significant prognostic factors for overall survival in a stepwise multivariate analysis were irradiation dose (p = 0.0001), number of chemotherapy cycles (p = 0.001), and preoperative KPS (p = 0.05); for progression-free survival it was number of chemotherapy cycles (p = 0.004). Survival was not affected by excision size, radiation method, histopathologic type of tumor, gender, ethnic origin, or time lapsed between surgery and irradiation.
A Method for Determining Critical Swimming Velocity

The purpose of this study was to determine whether the critical swimming velocity (Vcri) estimated by the swimming velocity for a distance of 300 m at maximal effort breaststroke reflects the maximal lactate steady state (MLSS). Twelve trained swimmers swam 50 m, 300 m and 2 000 m at maximal effort for determination of Vcri that averaged 1.167 +/- 0.045 m . sec (-1). Since Vcri was equivalent to 90.5 % of the mean swimming velocity over the distance of 300 m at maximal effort, the swimming velocity obtained by multiplying the swimming velocity for the distance of 300 m of each subject by 90.5 % was taken to be 100 % of the predicted critical swimming velocity (Vcri-pred). Then, in an MLSS test, the subjects were instructed to swim breaststroke 2 000 m (5 x 400 m) at three constant velocities (98 %, 100 %, and 102 % of Vcri-pred), interrupted by four short rest periods from 30 to 45 seconds for blood sampling and heart rate measurement. As a result, the blood lactate concentration at 100 % Vcri-pred showed a higher steady state than the slow velocity, but at high velocity did not show the steady state. In conclusion, we can accurately estimate the Vcri for breaststroke by a one-time 300-m maximal effort swimming test.
Recurrent non-specific low-back pain in adolescents: the role of exercise

This study evaluated the efficacy of an exercise programme as an intervention for recurrent non-specific low-back pain (NLSBP) in adolescents. A randomized controlled trial was conducted with an experimental group (n = 27, age 14.6 years) who participated in an 8-week exercise programme and a matched control group (n = 27, age 14.6 years) who continued normal daily activities. All participants suffered from recurrent NSLBP. Pre and post intervention measures of NSLBP status (pain severity and consequences) and daily inactivity (time spent sitting, PC time, TV time) were reported in one week diaries. Two-way mixed ANOVA (independent variables: pre/post and experimental/control) was conducted for each dependent variable, significance was set at P < 0.05. Significant interaction effects were identified for the severity of pain, number of occasions missing sport due to NSLBP and amount of sport participated in. In each case the experimental group benefited from the exercise programme. In contrast, no significant interaction effects were observed for physical inactivity, both groups spent a similar amount of time sitting, watching TV and using a PC pre- and post- intervention. It was concluded that an exercise programme acted as an effective short-term treatment strategy for NSLBP in adolescents. Further evaluation is required to assess the long-term effectiveness.
An XQuery-Based Language for Processing Updates in XML

Extensive usage of XML for information exchange and data processing has led to the development of standard languages for querying and publishing of XML data. However, a key problem in XML data management is the absence of a standard language to update XML. As a result, updates are carried out in a mostly ad-hoc fashion at the application level by writing code to modify tree-based structures representing XML documents in memory. So far very little research has been conducted on language-based updates and their impact on XML processing. In this paper we present UpdateX, our implementation of a declarative update language that can be used to directly update XML data using queries. Our update language is based on XQuery 1.0, W3C’s XML query language, and seamlessly integrates with all of its constructs and capabilities. We describe the user-level syntax of our update language and present a framework for its implementation. The UpdateX prototype, which has been implemented on top of the Galax XQuery 1.0 processor, is fully functional and its source code distribution is publicly available on the Web.
Differential Expression of Inducible Costimulator-Ligand Splice Variants: Lymphoid Regulation of Mouse GL50-B and Human GL50 Molecules

The process of immunological costimulation between APC and T cells is mediated by protein ligand:receptor interactions. To date, costimulatory receptors known to be expressed by T cells include the structurally related proteins CD28 and the inducible costimulator (ICOS). The ligands to human and mouse ICOS, human GL50 (hGL50), and mouse GL50 (mGL50) were recently cloned and demonstrated to have sequence similarity to the CD28 ligands B7-1 and B7-2. Examination of mGL50 cDNA transcripts by 3′RACE revealed an alternatively spliced form, mGL50-B, that encoded a protein product with a divergent 27-aa intracellular domain. Both mGL50- and mGL50-B-transfected cells exhibited binding to human and mouse ICOS-Ig fusion protein, indicating that the alternate cytoplasmic domain of mGL50-B does not interfere with extracellular interactions with ICOS receptor. Flow cytometric and RT-PCR analysis of BALB/c and RAG1−/− mice splenocytes demonstrate that freshly isolated B cells, T cells, macrophages, and dendritic cells express both splice variant forms of ICOS ligand. Comparative analyses with the human ICOS ligand splice variants hGL50 and B7-H2 indicate that differential splicing at the junction of cytoplasmic exon 6 and exon 7 may be a common method by which GL50-ICOS immunological costimulatory processes are regulated in vivo.
Polymorphism in ospC gene of Borrelia burgdorferi and immunoreactivity of OspC protein: implications for taxonomy and for use of OspC protein as a diagnostic antigen

The nucleotide sequences of the ospC gene from five Danish human Borrelia burgdorferi isolates representing all three B. burgdorferi genospecies (B. burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461) and from the American type strain B31 were determined and compared with the published ospC sequence from the German B. burgdorferi isolate PKo (R. Fuchs, S. Jauris, F. Lottspeich, V. Preac-Mursic, B. Wilske, and E. Soutschek, Mol. Microbiol. 6:503-509, 1992). The ospC gene was present in all isolates, regardless of the presence or absence of its product, OspC. The deduced amino acid sequences of OspC from the seven isolates were aligned and revealed pairwise sequence identities ranging from 60.5 to 100%. Differences were scattered throughout the amino acid sequences. A phylogenetic tree was constructed and revealed three distinct phenotypic groups OspCI to OspCIII corresponding to the three delineated genospecies. Immunoblot analysis revealed that the seven OspC proteins tested have both common and specific epitopes. There is significant epitope diversity, since even polyclonal antisera showed serotype-restricted specificity. Therefore, a serodiagnostic assay for Lyme borreliosis utilizing OspC as a test antigen should include all three OspC phenotypes in order to obtain a species-wide sensitivity.
Parental representation in young women who attempt suicide

ABSTRACT– A reliable and valid Parental Bonding Instrument was administered to young women who had attempted suicide. Results indicated low parental care and high overprotection. These results are consistent with theoretical formulations of suicidal behaviour, and taken with other studies help provide a profile of parental characteristics which may be associated with such behaviour.
Deciphering the Genes for Taste Receptors for Fructose in Drosophila

Taste sensitivity to sugars plays an essential role in the initiation of feeding behavior. In Drosophila melanogaster, recent studies have identified several gustatory receptor (Gr) genes required for sensing sweet compounds. However, it is as yet undetermined how these GRs function as taste receptors tuned to a wide range of sugars. Among sugars, fructose has been suggested to be detected by a distinct receptor from other sugars. While GR43A has been reported to sense fructose in the brain, it is not expressed in labellar gustatory receptor neurons that show taste response to fructose. In contrast, the Gr64a-Gr64f gene cluster was recently shown to be associated with fructose sensitivity. Here we sought to decipher the genes required for fructose response among Gr64a-Gr64f genes. Unexpectedly, the qPCR analyses for these genes show that labellar expression levels of Gr64d and Gr64e are higher in fructose low-sensitivity flies than in high-sensitivity flies. Moreover, gustatory nerve responses to fructose in labellar sensilla are higher in Gr64d and Gr64f mutant lines than in mutant flies of the other Gr64a-Gr64f genes. These data suggest the possibility that deletion of GR64D or GR64F may indirectly induce enhanced fructose sensitivity in the labellum. Finally, we conclude that response to fructose cannot be explained by a single one of the Gr64a-Gr64f genes.
Cervical cord and ventricle affection in neuromyelitis optica

Cervical cord involvement is common in neuromyelitis optica (NMO) and multiple sclerosis (MS), but its impact on disability in NMO has rarely been studied. Recent publications on NMO examined the periventricular system, areas of high aquaporin‐4 expression, but not yet by using ventricle volumetry.
Radionuclide concentrations in samples of medicinal herbs and effective dose from ingestion of 137Cs and natural radionuclides in herbal tea products from Serbian market

The objective of this study is to evaluate natural and artificial radioactivity levels in 18 different samples of medicinal herbs from Serbian market. The activity concentrations of 238U, 232Th, 40K, and 137Cs were measured using gamma spectroscopy system with a high-purity germanium detector. The activity concentrations of 238U, 232Th, 40K, and 137Cs were found to be in a range of 0.6–8.2, 1.7–15.1, 126–1243.7, and 0.3–8.8 Bq/kg, respectively. The annual whole body doses from ingestion of 137Cs and natural radionuclides for an adult person who consumed herbal tea from these medicinal herbs were found to be 2.5–469.9 nSv for 137Cs, 0.7–9.7 nSv for 238U, 0.3–2.8 nSv for 232Th, and 1026.0–10,132.0 nSv for 40K. These doses are not hazardous to public health. The obtained results were compared with correspondent studies conducted worldwide.