Miracle-dz/UniFAR-FTUs huggingface dataset:
Intraoperative Touch Imprint and Frozen Section Analysis of Sentinel Lymph Nodes After Neoadjuvant Chemotherapy for Breast Cancer

Objective:To evaluate the accuracy of touch imprint and frozen section analysis of sentinel nodes after neoadjuvant chemotherapy. Summary Background Data:Intraoperative evaluation of the sentinel node can determine the need for axillary dissection at the time of initial operation and therefore spare the patient a second operation. Little data, however, exists on the accuracy of intraoperative evaluation of sentinel nodes after neoadjuvant chemotherapy. Methods:A retrospective chart review was performed of all sentinel node procedures for breast cancer from 2004 to 2008 at a single institution. The sentinel node procedure was done before (no-NACT) chemotherapy in 107 patients and 37 had the procedure after neoadjuvant (NACT) chemotherapy. Intraoperative analysis of sentinel nodes was performed using touch imprint and frozen section techniques. Results:In the no-NACT group, intraoperative assessment by touch imprint analysis had 61% sensitivity, 100% specificity, and 87% accuracy. Frozen section analysis was similar with 74% sensitivity, 100% specificity, and 90% accuracy. In the NACT group, touch imprint analysis had 79% sensitivity, 100% specificity, and 90% accuracy. Frozen section analysis was again similar with 74% sensitivity, 100% specificity, and 83% accuracy. When the no-NACT group and the NACT group were compared, both frozen section and touch imprint analysis had similar sensitivity, specificity, and accuracy. Conclusions:Intraoperative evaluation of sentinel nodes with touch imprint and frozen section analysis in patients treated with neoadjuvant chemotherapy showed acceptable sensitivity, specificity, and accuracy. Nearly all of the misses in intraoperative evaluation were in patients with micrometastases.
‘Adipaging’: ageing and obesity share biological hallmarks related to a dysfunctional adipose tissue

The increasing ageing of our societies is accompanied by a pandemic of obesity and related cardiometabolic disorders. Progressive dysfunction of the white adipose tissue is increasingly recognized as an important hallmark of the ageing process, which in turn contributes to metabolic alterations, multi‐organ damage and a systemic pro‐inflammatory state (‘inflammageing’). On the other hand, obesity, the paradigm of adipose tissue dysfunction, shares numerous biological similarities with the normal ageing process such as chronic inflammation and multi‐system alterations. Accordingly, understanding the interplay between accelerated ageing related to obesity and adipose tissue dysfunction is critical to gain insight into the ageing process in general as well as into the pathophysiology of obesity and other related conditions. Here we postulate the concept of ‘adipaging’ to illustrate the common links between ageing and obesity and the fact that, to a great extent, obese adults are prematurely aged individuals.
Identification of novel biomass‐degrading enzymes from genomic dark matter: Populating genomic sequence space with functional annotation

Although recent nucleotide sequencing technologies have significantly enhanced our understanding of microbial genomes, the function of ∼35% of genes identified in a genome currently remains unknown. To improve the understanding of microbial genomes and consequently of microbial processes it will be crucial to assign a function to this “genomic dark matter. ” Due to the urgent need for additional carbohydrate‐active enzymes for improved production of transportation fuels from lignocellulosic biomass, we screened the genomes of more than 5,500 microorganisms for hypothetical proteins that are located in the proximity of already known cellulases. We identified, synthesized and expressed a total of 17 putative cellulase genes with insufficient sequence similarity to currently known cellulases to be identified as such using traditional sequence annotation techniques that rely on significant sequence similarity. The recombinant proteins of the newly identified putative cellulases were subjected to enzymatic activity assays to verify their hydrolytic activity towards cellulose and lignocellulosic biomass. Eleven (65%) of the tested enzymes had significant activity towards at least one of the substrates. This high success rate highlights that a gene context‐based approach can be used to assign function to genes that are otherwise categorized as “genomic dark matter” and to identify biomass‐degrading enzymes that have little sequence similarity to already known cellulases. The ability to assign function to genes that have no related sequence representatives with functional annotation will be important to enhance our understanding of microbial processes and to identify microbial proteins for a wide range of applications. Biotechnol. Bioeng. 2014;111: 1550–1565. © 2014 Wiley Periodicals, Inc.
Estimations of Bacterial Growth Rates in Natural Waters

Specific growth rates as low as 0.005 hr−1 (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterilization) and by competition with the natural microflora for the unknown growth-limiting substrate.
Weights for Computing Vertex Normals from Facet Normals

Abstract I propose a new equation to estimate the normal at a vertex of a polygonal approximation to a smooth surface, as a weighted sum of the normals to the facets surrounding the vertex. The equation accounts for the difference in size of these facets by assigning larger weights for smaller facets. When tested on random cubic polynomial surfaces, the equation is superior to other popular weighting methods.
HIV knowledge and condom intention among sexually abstinent Chinese students.

AIMS To (a) assess the HIV/AIDS knowledge and sources of HIV/sexually transmitted infection (STI) information among sexually abstinent college students in China; (b) examine whether constructs from the transtheoretical model (TTM) are applicable to this study population regarding condom use intention; and (c) evaluate the association between genders and TTM constructs, and HIV/AIDS knowledge. BACKGROUND Chinese college students are vulnerable to HIV and other STIs. Strategies targeting abstinent students are more cost-effective than providing treatment for diseases. METHODS We surveyed 390 students enrolled in two universities in China. Data were collected from June 2009 to March 2010. RESULTS Only 11% and 24% were aware of HIV spread by infected semen and of the protective effects of condom use against HIV. The mass media were major sources of HIV/STI information. Individuals who had higher levels of self-efficacy and reported more perceived benefits and fewer perceived barriers were more likely to be in TTM contemplation stage of condom use than those in precontemplation. Females were less likely to discuss HIV/STIs through online chat or email with strangers than males. Individuals who had higher levels of self-efficacy and reported more perceived benefits and fewer perceived barriers were more likely to be in TTM contemplation stage of condom use than those in precontemplation. CONCLUSION Sexually abstinent college students in China may be more likely to transition from precontemplation to contemplation if they know the benefits of condom use for the prevention of HIV/STIs and if they learn to successfully minimize potential barriers related to condom use.
Effect of a Periodized Power Training Program on the Functional Performances and Contractile Properties of the Quadriceps in Sprinters

Our purpose was to compare the effect of a periodized preparation consisting of power endurance training and high-intensity power training on the contractile properties of the quadriceps muscle and functional performances in well trained male sprinters (n = 7). After 4 weeks of high-intensity power training, 60-m sprint running time improved by an average of 1.83% (SD = 0.96; p < .05). This improvement was inversely related to an increase in maximal voluntary contraction torque (r = -.89, p < .05) and poorly correlated with changes in the contractile kinetics of the quadriceps muscle (r range from .36 to -.46). These findings suggest that sprint performance is poorly predicted by muscle intrinsic properties and that a neural adaptation appears to explain most of the observed functional adaptations.
Comparison of Dynamic Contour Tonometry and Goldmann Applanation Tonometry in Keratoconus

Purpose: We performed a comparative study using Goldmann applanation tonometry (GAT) and dynamic contour tonometry (DCT) to measure intraocular pressure (IOP) in eyes with keratoconus. Methods: IOP was measured in 114 eyes of 75 patients with keratoconus (51 men and 24 women; mean age, 36.1 ± 11.2 years) using GAT and DCT in randomized order. Central corneal thickness (CCT), minimal corneal thickness (MCT), and corneal topography were recorded using the Pentacam. Four groups according to Amsler's keratoconus classification were composed and analyzed for significant differences of CCT, MCT, GAT, and DCT results. Results: Mean CCT in the 114 keratoconus eyes was 481.1 ± 46.2 μm (range, 334-601 μm). Mean MCT was 453.3 ± 56.3 μm (range, 239-573 μm). Mean IOP measured using GAT was 13.1 ± 2.9 mm Hg, whereas mean IOP measured using DCT was 14.8 ± 2.6 mm Hg. Neither the results for GAT nor those for DCT showed a significant correlation with CCT (Pearson correlation: P < 0.05). Multifactorial analysis revealed that CCT and MCT, but not GAT and DCT, results were significantly different in corneas of varying curvatures. Conclusions: This study shows that DCT measures IOP higher than GAT in eyes with keratoconus. In keratoconus, both methods seem to be independent of CCT and therefore are equally, but not interchangeably, applicable when monitoring IOP. Further analysis revealed that CCT and MCT are significantly different in corneas of varying Amsler grade.
Effects of homogeneous and variable exposure on magnitude of adaptation to optical tilt.

Repeated exposure to hallway exploration, alternated with periods of either watching the active hand or exploring a different set of hallways, maintains increasing visual adaptation beyond the point in time at which previous studies using homogeneous exposure have found such visual shift (VS) to be asymptotic. Experiment 1 established that this alternation-repetition effect does not depend on an actual change in task (hall to hand) but also occurs when the task context alone is changed (hall to hall). Experiment 2 compared variable (hall-to-hall) exposure with homogeneous (hall) exposure, showing that variable exposure removes the usual limit on adaptation. In both experiments, proprioceptive shift (PS) and total negative aftereffect (NA) both tended to be less than VS, producing substantial overadditivity (i.e., VS + PS greater than NA). General requirements for an attentional explanation of the alternation-repetition effect are outlined, and possible explanations of overadditivity consistent with the linear model are discussed.
Lumbar Spine Orthosis Wearing: I. Restriction of Gross Body Motions

The effects of wearing commonly prescribed low-back braces and corsets on restriction of gross body motions were investigated. A lumbosacral corset, a chairback brace, and a molded plastic thoracolumbosacral orthosis (TLSO) were studied. Four trunk movements (flexion, extension, lateral bending, and twisting) were examined in five healthy adult men when standing and sitting. All three orthoses restricted at least some gross body motion to approximately two thirds to one half of no-orthosis values. All three orthoses failed to provide restrictions of at least 10% in at least one motion. Mean motion restriction across all eight movements studied in all five subjects were largest when wearing the TLSO and least when wearing the corset. Gross body motion restrictions relieve lumbar trunk muscle and spine loads.
Use of etomidate as an induction agent for rapid sequence intubation in a pediatric emergency department.

OBJECTIVES Although etomidate is widely used for rapid sequence intubation (RSI), there is no consensus on the optimal induction agent and no prospective pediatric emergency department (ED) study exists. The objective of this study was to assess the effectiveness and safety of etomidate as an induction agent for RSI in the pediatric ED. METHODS Data on RSI conditions and complications were collected prospectively on patients undergoing RSI in a tertiary pediatric ED from January 2003 to December 2003. ED hemodynamic data and inpatient data were collected retrospectively via chart review. RESULTS Seventy-seven of 101 patients requiring intubation underwent RSI with etomidate. The mean (+/- SD) age was 8.2 (+/- 6.2) years. All 77 patients were successfully intubated. Intubation condition data were available for 69 of 77 patients (89.6%). Conditions were good in 68 of 69 (99%; 95% confidence interval = 92.2% to 99.9%). The mean (+/- SD) maximal percent decrease in systolic blood pressure was 10% (+/- 13.6%). A greater than 20% maximal percent decrease in systolic blood pressure occurred in 12 of 69 patients (17.4%; 95% confidence interval = 9.3% to 28.4%). There was no relationship between seizures after etomidate administration and prior seizure history (p = 0.25). Corticosteroids were given to 29 of 77 patients post-RSI for varying diagnoses. All eight patients given corticosteroids for shock were in shock at the time of intubation. CONCLUSIONS In the pediatric ED setting, etomidate as an induction agent provided successful RSI conditions and resulted in varied hemodynamic changes that were especially favorable in those patients presenting in decompensated shock. Hypotension and seizures were uncommon and occurred in patients with confounding diagnoses. Until the significance of a single dose of etomidate on adrenal dysfunction is further clarified, caution should be used in those patients at risk for adrenal insufficiency.
AN ALTERNATIVE MODE OF ACTION OF ENDOCRINE-DISRUPTING CHEMICALS AND CHEMOPREVENTION

Endocrine-disrupting chemicals (EDCs) produce a wide variety of endocrine-disruption effects, including developmental disorders, carcinogenicity, and mutagenicity. Our recent study demonstrated that about 94% of the 48 EDCs classified by Centers for Disease Control and Prevention (CDC) generated free radicals and that this free radical generation induced by EDCs might represent a common toxic mechanism of action of EDCs. The chemoprevention of endocrine-disrupting effects, such as employed in the control of caner by interfering with common toxic mechanisms of action of EDCs, represents a promising approach to this problem. In this context, it is proposed that EDCs may produce endocrine-disrupting effects including carcinogenicity via the generation of free radicals, and thus the effects may be modulated or prevented by scavenging free radicals with antioxidants, such as vitamins, curcumin, isoflavonoids, resveratrol, and plant polysaccharides. Here, an alternative mode of action of EDCs and their possible chemoprevention are proposed.
A Rat’s Whiskers Point the Way toward a Novel Stimulus-Dependent, Protective Stroke Therapy

Stroke is the fourth leading cause of death in the United States and the leading cause of long-term disability. Ischemic stroke, due to an interruption in blood supply, is particularly prevalent; 87% of all strokes are ischemic. Unfortunately, current options for acute treatment are extremely limited and there is a great need for new treatment strategies. This review will discuss evidence that mild sensory stimulation can completely protect the jeopardized brain from an impending stroke in a rodent model. When delivered within the first 2 hours following ischemic onset, this stimulation results in complete protection, including a full reestablishment of cortical function, sensorimotor capabilities, and blood flow. Identical stimulation, however, initiated 3 hours following ischemic onset, results in an increase in damage compared with untreated animals. The protective effect is not specific to a single sensory modality, anesthesia, or age, and increasing evoked cortical activity by increasing stimulation accelerates recovery. Taken together, these findings demonstrate that cortical activity is a critical factor for protection and suggest a new, exciting potential avenue for the development of acute stroke treatment strategies that may produce a noninvasive, drug-free, equipment-free, and side effect–free means of protecting from ischemic stroke.
Adsorption of different laccases on cellulose and lignin surface

The adsorption of Trametes hirsuta and Melanocarpus albomyces laccases on cellulose and lignin model substrates was studied by quartz crystal microbalance with dissipation, QCM-D. The laccase-treated surfaces were also analyzed by atomic force microscopy (AFM) and x-ray photoelectron spectroscopy (XPS). The laccases were found to adsorb at acidic and neutral pHs on both surfaces. The adsorbed amounts increased rather linearly with the change in dissipation when the lignin or cellulose was treated with T. hirsuta laccase. Higher adsorbed amounts were obtained using M. albomyces laccase. The adsorption of M. albomyces laccase on lignin was strongly dependent on pH. At low pH thin and rigid laccase layers were formed and the amount adsorbed was high, while at high pH the laccase layers formed were dissipative and loose and the amount adsorbed was low. A good correlation between the adsorbed amount of laccase and the surface nitrogen content was found. The adsorption of laccases made the surface structure of the cellulose and lignin substrates more granular.
Family medicine specialty selection: a proposed research agenda.

BACKGROUND AND OBJECTIVES Our objectives were to provide an update on the status of selection by medical students of family medicine as a specialty, to summarize what is known about influences on this specialty choice, and to propose a research agenda for the future. METHODS Data from the National Resident Matching Program and the American Academy of Family Physicians residency database were obtained. Past reviews of research on family medicine specialty selection were summarized, and recent research was reviewed. Gaps in the knowledge base and common research design weaknesses were listed. RESULTS As a result of our analysis, a set of 12 research questions are posed and a set of 11 study design recommendations made. CONCLUSIONS Family medicine specialty choice research must be planned and conducted with the most robust methodology possible, building on past research, to produce results that can assist in formulating effective policy.
The bacterial cell‐division protein ZipA and its interaction with an FtsZ fragment revealed by X‐ray crystallography

In Escherichia coli, FtsZ, a homologue of eukaryotic tubulins, and ZipA, a membrane‐anchored protein that binds to FtsZ, are two essential components of the septal ring structure that mediates cell division. Recent data indicate that ZipA is involved in the assembly of the ring by linking FtsZ to the cytoplasmic membrane and that the ZipA‐FtsZ interaction is mediated by their C‐terminal domains. We present the X‐ray crystal structures of the C‐terminal FtsZ‐binding domain of ZipA and a complex between this domain and a C‐terminal fragment of FtsZ. The ZipA domain is a six‐stranded β‐sheet packed against three α‐helices and contains the split β‐α‐β motif found in many RNA‐binding proteins. The uncovered side of the sheet incorporates a shallow hydrophobic cavity exposed to solvent. In the complex, the 17‐residue FtsZ fragment occupies this entire cavity of ZipA and binds as an extended β‐strand followed by α‐helix. An alanine‐scanning mutagenesis analysis of the FtsZ fragment was also performed, which shows that only a small cluster of the buried FtsZ side chains is critical in binding to ZipA.
Energy conservation goals: What people adopt, what they recommend, and why

Failures to reduce greenhouse gas emissions by adopting policies, technologies, and lifestyle changes have led the world to the brink of crisis, or likely beyond. Here we use Internet surveys to attempt to understand these failures by studying factors that affect the adoption of personal energy conservation behaviors and also endorsement of energy conservation goals proposed for others. We demonstrate an asymmetry between goals for self and others (“I’ll do the easy thing, you do the hard thing”), but we show that this asymmetry is partly produced by actor/observer differences: people know what they do already (and generally do not propose those actions as personal goals) and also know their own situational constraints that are barriers to action. We also show, however, that endorsement of conservation goals decreases steeply as a function of perceived difficulty; this suggests a role for motivated cognition as a barrier to conservation: difficult things are perceived as less applicable to one’s situation.
Induction of a fast inactivation gating on delayed rectifier Shab K+ channels by the anti-inflammatory drug celecoxib

Celecoxib is a drug designed to selectively inhibit COX-2, an inflammation-inducible cyclooxygenase isoform, over the constitutively expressed COX-1 isoform. In addition to this selective inhibition it is now known that celecoxib exerts a variety of effects on several types of ion channels, thus producing secondary physiological effects. In this work we demonstrate that at therapeutically relevant concentrations celecoxib interacts with Shab K+ channels specifically promoting a fast inactivation gating (without blocking the pore or significantly affecting other gating processes). At least two celecoxib molecules bind to each channel promoting a fast inactivation that develops from both open and closed states. Channel inactivation in turn causes a reduction of the size of IK. Taken together, our observations show that in addition to its intended therapeutic target celecoxib is a useful tool to further study the mechanism of Shab channel inactivation.