Miracle-dz/UniFAR-FTUs huggingface dataset:
Chronic hepatitis C virus infection: it is not only about the liver

Background and aims Although hepatitis B virus (HBV) and hepatitis C virus (HCV) are both hepatotropic and quite similar in terms of clinical manifestations and histopathology, their respective infections are distinct in terms of epidemiology and prognosis. Recognizing the differences between patients with HBV and HCV infection with respect to demographic characteristics, prevalence of comorbidities, and presence of lifestyle factors aids the proper treatment of these patients. We aimed to compare two populations with chronic viral liver disease (chronic HCV and chronic HBV), each of them with resolved hepatitis C. Patients and methods We included patients referred to a municipal reference clinic from March 2009 through May 2012. Patient data were collected using standardized questionnaires at the patients’ first visit to clinic. Questionnaires included epidemiological information, presence of comorbidities, and lifestyle. Results A total of 756 patients were included in the study, 348 (46.0%) with chronic HCV infection, 176 (23.3%) with chronic HBV infection, and 232 (30.7%) with resolved HCV infection. Multivariate analysis including patients with chronic HCV infection and chronic HBV infection indicated that age [adjusted odds ratio (AOR)=1.06; 95% confidence interval (CI): 1.03–1.08], alcohol abuse (AOR=1.58; 95% CI: 1.01–2.49), smoking (AOR=1.64; 95% CI: 1.00–2.17), and illicit drug (AOR=2.92; 95% CI: 1.69–5.02) use were associated independently with chronic HCV infection. Multivariate analyses including patients with chronic HCV infection and those patients with resolved HCV infection, presence of at least one comorbidity (AOR=1.94; 95% CI: 1.12–3.3), illicit drug use (AOR=3.24; 95% CI: 1.90–5.54), and age (AOR=1.03; 95% CI: 1.01–1.05) were independently associated with chronic HCV infection. Age (AOR=0.98; 95% CI: 0.96–0.99) and male sex (AOR=1.93; 95% CI: 1.26–2.95) were the only variables associated significantly with chronic HBV infection in the multivariate analysis between patients with chronic HBV infection and resolved HCV infection. Conclusion Our results highlight that patients with chronic HCV infection are complex and require a multidisciplinary approach during patient follow-up and clinical management.
Water Productivity Evaluation under Multi-GCM Projections of Climate Change in Oases of the Heihe River Basin, Northwest China

As the second largest inland river basin situated in the middle of the Hexi Corridor, Northwest China, the Heihe River basin (HRB) has been facing a severe water shortage problem, which seriously restricts its green and sustainable development. The evaluation of climate change impact on water productivity inferred by crop yield and actual evapotranspiration is of significant importance for water-saving in agricultural regions. In this study, the multi-model projections of climate change under the three Representative Concentration Pathways emission scenarios (RCP2.6, RCP4.5, RCP8.5) were used to drive an agro-hydrological model to evaluate the crop water productivity in the middle irrigated oases of the HRB from 2021–2050. Compared with the water productivity simulation based on field experiments during 2012–2015, the projected water productivity in the two typical agricultural areas (Gaotai and Ganzhou) both exhibited an increasing trend in the future 30 years, which was mainly attributed to the significant decrease of the crop water consumption. The water productivity in the Gaotai area under the three RCP scenarios during 2021–2050 increased by 9.2%, 14.3%, and 11.8%, while the water productivity increased by 15.4%, 21.6%, and 19.9% in the Ganzhou area, respectively. The findings can provide useful information on the Hexi Corridor and the Belt and Road to policy-makers and stakeholders for sustainable development of the water-ecosystem-economy system.
Place cell assemblies remain intact, despite reduced phase precession, after cholinergic disruption

The hippocampal theta rhythm is frequently viewed as a clocking mechanism that coordinates the spiking activity of neurons across the hippocampus to form coherent neural assemblies. Phase precession is a form of temporal coding evidencing this mechanism and is degraded following systemic pharmacological disruption of cholinergic signaling. However, whether neural assemblies are commensurately degraded, as would be predicted from a clocking mechanism hypothesis, remains unknown. To address this, we recorded the spiking activity of hippocampal place cells as rats completed laps on a circle track for chocolate drink before versus during the influence of a systemic muscarinic acetylcholine receptor antagonist. We compared the integrity of hippocampal ensembles using three approaches. The first approach used cross‐correlogram (CCG) analyses to ask if the relative spike‐timing between pairs of cells became less reliable. The second used a general linear model based analysis to ask whether the activity of simultaneously recorded neurons became any less predictive of the spiking activity of single neurons. Finally, the third approach used a reconstruction analysis to ask if the population activity was any less informative regarding the environmental position of the animal and whether theta sequences were impaired. The results of all three analyses paint a consistent picture: systemic cholinergic disruption did not degrade assembly integrity. These data demonstrate that place cell assemblies do not depend upon high quality phase precession.
Prodigiosin Induces Caspase‐9 and Caspase‐8 Activation and Cytochrome C Release in Jurkat T Cells

Abstract: Prodigiosin (PG) is an active component of bacterial origin, with reported apoptotic effects. We examined the activation of caspases‐9, ‐8, and ‐3 in PG‐treated Jurkat cells in a dose‐response study. These caspases were activated in apoptotic cells, as judged by the appearance of cleavage products from procaspases, and cytochrome c (cyt c) was released to the cytosol. In addition, PG induced the cleavage of poly (ADP‐ribose) polymerase (PARP). This study demonstrates that the activation of caspases‐9, ‐8, and ‐3 mediates PG‐induced apoptosis.
Characterization of cDNAs induced in meiotic prophase in lily microsporocytes.

To identify and analyze genes functioning during reproductive cell formation in higher plants, cDNAs harboring the messages induced in meiotic prophase were isolated and characterized. A cDNA library constructed from microsporocytes in meiotic prophase of Lilium longiflorum was screened with a subtraction probe specific to meiotic prophase. Clones selected were classified into 18 groups by cross hybridization and partial sequencing. Northern blot analysis revealed that the transcripts corresponding to the respective cDNA groups began accumulating at the early stages of meiosis and exhibited clone-specific profiles during meiosis and the spore formation process. The amino acid sequences of the predicted gene products showed similarity with known gene products, e.g. heat shock proteins, serine proteases in Bacillus, and RAD 51 gene product in yeast. Half of the putative gene products had hydrophobic N-terminal regions, suggesting that they may function as signal peptides.
Evidence for short-term, but not long-term, transfer effects in the temporal preparation of auditory stimuli

Starting procedures in racing sports consist of a warning (e.g., “Set”) followed by a target (e.g., “Go”) signal. During this interval (the foreperiod), athletes engage in temporal preparation whereby they prepare to respond to the target as quickly as possible. Despite a long history, the cognitive mechanisms underlying this process are debated. Recently, it has been suggested that traces of previous temporal durations drive temporal preparation performance rather than the traditional explanation that performance is related to the currently perceived hazard function. Los and colleagues used visual stimuli for the warning and target signals. As racing sports typically rely upon auditory stimuli, we investigated the role of memory on temporal preparation in the auditory domain. Experiment 1 investigated long-term transfer effects. In an acquisition phase, two groups of participants were exposed to different foreperiod distributions. One week later, during a transfer phase, both groups received the same distribution of foreperiods. There was no evidence for transfer effects. Therefore, Experiment 2 examined short-term transfer effects in which acquisition and transfer phases were completed in the same testing session. There was some evidence for transfer effects, but this was limited, suggesting that there may be modality-specific memory differences.
Are Socially Anxious Children Poor or Advanced Mindreaders?

Why are some children more socially anxious than others? One theory holds that socially anxious children are poor mindreaders, which hampers their social interactions; another that socially anxious children are advanced mindreaders leading to heightened self‐consciousness in social situations. To test these theories simultaneously, this study (N = 105, ages 8–12) assessed children's mindreading (accuracy in detecting mental states from the eye region), self‐consciousness (indexed as physiological blushing during public performance), and social anxiety levels. Results support both theories, showing a quadratic relation between mindreading and social anxiety. Low mindreading was related to clinical levels of social anxiety. High mindreading was related to subclinical levels of social anxiety through blushing. Our findings suggest two social‐cognitive pathways to heightened social anxiety.
Relationship between brachial and popliteal artery low-flow mediated constriction in older adults: Impact of aerobic fitness on vascular endothelial function.

We previously observed that brachial artery (BA) low-flow mediated constriction (L-FMC) is inversely related to aerobic fitness (i.e., VO2peak) in older adults (OA). However, it is unclear if an L-FMC response is elicited in the popliteal artery (POP) or if a similar inverse relationship with aerobic fitness exists. Considering that the POP experiences larger shear stress fluctuations during sedentary behaviors and traditional lower limb modes of aerobic exercise, we tested the hypotheses that: 1) heterogeneous L-FMC responses exist between the BA vs. POP of OA, and 2) that aerobic fitness will be inversely related to POP L-FMC. L-FMC was assessed in 47 healthy OA (30♀, 67 ± 5 yr) using duplex ultrasonography and quantified as the percent decrease in diameter (from baseline) during the last 30 s of a 5 min distal cuff occlusion period. When allometrically scaled to baseline diameter, the BA exhibited a greater L-FMC response than the POP (-1.3±1.6% vs. -0.4±1.6%; P=0.03). Furthermore, L-FMC responses in the BA and POP were not correlated (r=0.22; P=0.14). VO2peak was strongly correlated to POP L-FMC (r=-0.73; P<0.001). The heterogeneous BA versus POP L-FMC data indicate that upper limb L-FMC responses do not represent a systemic measure of endothelial-dependent vasoconstrictor capacity in OA. The strong association between VO2peak and POP L-FMC suggests that localized shear stress patterns, perhaps induced by lower limb dominant modes of aerobic exercise, may result in greater vasoconstrictor responsiveness in healthy OA.
The Epileptic Mother and Her Child

Summary: This study is a review of the literature on the influence of epilepsy and antiepileptic medication on the course of pregnancy and the foetus, as well as of the effect of pregnancy on the disease. Antiepileptic treatment is supposedly responsible for the increased rate of abnormal bleeding after delivery. Data on the perinatal mortality of epileptics are conflicting. The offspring of epileptic women are often smaller than normal neonates, indicating retarded growth, and the incidence of malformations is 1.25 times higher. Until now there has been no direct evidence of a teratogenic effect of the commonly used antiepileptic drugs, and the risk of malformations may possibly be correlated to the severity of the epilepsy and the disease per se. The effect of pregnancy on seizure frequency seems to be quite variable. Higher seizure frequency is seen during pregnancy, but this is not a universal phenomenon. The incidence of seizures in gestational epilepsy has no predictive value concerning the course of later pregnancies. A positive correlation seems to exist between the development of eclampsia and later epilepsy, a family history of epilepsy and cerebral dysrhytmias. Status epilepticus is uncommon in pregnancy. The requirement of several antiepileptic drugs is increased during pregnancy. And the metabolism of carbamazepine, primidone, and clorazepate is probably accelerated in the gestational period. Data on the metabolism of other antiepileptic drugs in this situation are inconclusive or lacking. The intestinal absorption of antiepileptic drugs may be impaired during pregnancy. The reduced concentration of plasma proteins is probably only of minor importance. Placentally transferred antiepileptic drugs are usually cleared from the newborn within a week, but a risk of intoxication in the newborn exists when diazepam is administered to the mother at delivery. The concentration of antiepileptic drugs in breast milk is low, but careful observation of the newborn is important when this drug and phenobarbitone are administered to the mother. The epileptic pregnant woman demands special attention, including careful obstetric control and monitoring of plasma concentrations once a month during pregnancy and once a week post partum until plasma clearance has returned to prepregnancy values with subsequent dose adjustment. Close observation of the newborn is mandatory. In most instances, breast‐feeding can be carried out without any risk to the baby.
Temperamental vulnerability to emotion dysregulation and risk for mental and physical health challenges

Abstract Emotion dysregulation characterizes many forms of psychopathology. Patterns of dysregulation occur as a function of a developmental process in which normative and adaptive emotion regulation skills fail to become part of the child's behavioral repertoire due to biological, psychological, and contextual processes and experiences. Here we highlight the processes involved in the dysregulation of temperamental anger and frustration that become core features of externalizing problems and place children at risk for more serious forms of psychopathology. We imbed these processes in a larger self-regulatory framework, and we discuss how they influence mental as well as physical health, using data from our 20-year longitudinal study following a large cohort of children into young adulthood. Recommendations are made for future research involving the integration of biological systems with mental and physical health outcomes.
Efficient Face Detection using Adaboost

Face detection is an essential application of visual object detection and it is one of the main components of face analysis and understanding with face localization and face recognition. It becomes a more and more complex domain used in a large number of applications, among which we find security, new communication interfaces, biometrics and many others. The goal of face detection is to detect human faces in still images or videos, in different situations. We will focus on a detector which processes images very quickly while achieving high detection
Epidemiology of MRSA CC398 in hospitals located in Spanish regions with different pig-farming densities: a multicentre study.

BACKGROUND Tetracycline resistance (TetR) is a marker of livestock-associated MRSA of lineage CC398. OBJECTIVES To determine the MRSA CC398 prevalence among TetR-MRSA recovered in Spanish hospitals located in regions with different pig-farming densities, and the influence of pig density as a key risk factor for its acquisition. METHODS TetR-MRSA isolates (n = 232) recovered from clinical and epidemiological samples during January-June 2016 in 20 hospitals in 13 regions with different pig-farming densities were analysed. MRSA CC398 identification, detection of spa types, methicillin resistance genes and immune evasion cluster (IEC) genes were performed by PCR/sequencing. Statistical analyses were performed to establish the relationships between MRSA CC398 prevalence and pig density. RESULTS The global MRSA prevalence was 29.7% (6.9% TetR-MRSA/MRSA), with 137 CC398 isolates recovered, representing 4.1% of total MRSA and 59.1% of TetR-MRSA. Among MRSA CC398, 16 different spa types were recorded (t011: 72.3%), and all but two strains were IEC negative. Higher pig-density regions were associated with significant MRSA CC398 increases in hospitals located in adjacent regions (P < 0.001). Linear regression models explained the relationships between MRSA CC398 and pig density (P < 0.001), with an increase of 6.6 MRSA CC398 cases per 100 MRSA per increase of 100 pigs/km2 in a region. CONCLUSIONS High pig density leads to a significant increase in MRSA CC398 in hospitals in Spain, and its combination with a high human population could help its dissemination. In Spain, the prevalence of the zoonotic CC398 lineage is closely related to pig-farming density; therefore, specific tools could be implemented in order to detect its dissemination.
In vitro Acidogenic Potential and Mutans Streptococci of Human Smooth-surface Plaque Associated with Initial Caries Lesions and Sound Enamel

Samples of human dental plaque were pooled from several "white spot" smooth tooth surface areas as well as from several clinically-sound tooth surface areas in each of 12 caries-positive college students. Each of the two samples from each subject was used for the determination of: (1) pH-lowering potential in vitro involving dispersed plaque suspensions, excess glucose supply, and a 60-minute test, and (2) the proportions of mutans streptococci and lactobacilli. When all subjects were considered, plaques from "white spot" areas, as compared with samples from sound surface areas, were characterized by significantly higher proportions of mutans streptococci, a lower starting ("resting") pH, a faster rate of pH drop between pH 6.0 and 5.0, and a lower minimum pH of the suspension; the lactobacillus proportions were generally very low in both types of plaques. For individual subjects, however, the proportions of mutans streptococci in plaque associated with "white spot" areas showed a wide range (0.001-10.0%), and samples with high as well as low levels of these micro-organisms could exhibit a high rate of pH drop and a low pH minimum. This suggests that, besides mutans streptococci and lactobacilli, other bacteria capable of acidogenesis at a low pH may contribute to the high pH-lowering potential exhibited by many plaques.
Vascular Smooth Muscle Cells Contribute to Atherosclerosis Immunity

Vascular smooth muscle cells (VSMCs) constitute the major cells in the media layer of arteries, and are critical to maintain the integrity of the arterial wall. They participate in arterial wall remodeling, and play important roles in atherosclerosis throughout all stages of the disease. Studies demonstrate that VSMCs can adopt numerous phenotypes depending on inputs from endothelial cells (ECs) of the intima, resident cells of the adventitia, circulating immune cells, hormones, and plasma lipoproteins. This plasticity allows them to perform multiple tasks in physiology and disease. In this minireview, we focus on a previously underappreciated activity of VSMCs, i.e., their impact on atherosclerosis immunity via formation of artery tertiary lymphoid organs (ATLOs).
Concurrent Learning of Adjacent and Nonadjacent Dependencies in Visuo-Spatial and Visuo-Verbal Sequences

Both adjacent and non-adjacent dependencies (AD and NAD) are present in natural language and other domains, yet the learning of non-adjacent sequential dependencies generally only occurs under favorable circumstances. It is currently unknown to what extent adults can learn AD and NAD, presented concurrently in spatial and verbal sequences during a single session, and whether a second session improves performance. In addition, the relationship between AD and NAD learning and other theoretically related cognitive and language processes has not yet been fully established. In this study, participants reproduced two types of sequences generated from an artificial grammar: visuo-spatial sequences with stimuli presented in four spatial locations, and visuo-verbal sequences with printed syllables. Participants were tested for incidental learning by reproducing novel sequences, half consistent with the grammar and half containing violations of either AD or NAD. The procedure was repeated on a second day. Results showed that both AD and NAD were learned in both visuo-spatial and visuo-verbal tasks, although AD learning was better than NAD and learning of NAD decreased over time. Furthermore, NAD learning for both spatial and verbal tasks was positively correlated with a language measure, whereas AD learning for both spatial and verbal tasks was negatively associated with working memory measures in the opposite domain. These results demonstrate that adults can learn both AD and NAD within a single session, but NAD learning is more easily disrupted than AD and both types of learning are sub-served by partially distinct cognitive processes. These findings increase our understanding of the processes governing the learning of AD and NAD in verbal and spatial domains.
Adipose-Derived Mesenchymal Stromal Cells Under Hypoxia: Changes in Extracellular Vesicles Secretion and Improvement of Renal Recovery after Ischemic Injury.

BACKGROUND/AIMS The therapeutic potential of extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) in kidney injury has been largely reported. However, new approaches are necessary to optimize the efficacy in the treatment of renal diseases. MSCs physiologically are under a low O2 partial pressure (pO2), and culturing adipose-derived MSCs (ADMSCs) in hypoxia alters their secretory paracrine properties. The aim of this study was to evaluate whether hypoxia preconditioning of ADMSCs alters the properties of secreted EVs to improve renal recovery after ischemia-reperfusion injury (IRI). METHODS The supernatants of ADMSCs cultivated under 21% pO2 (control) or 1% pO2 (hypoxia) were ultracentrifuged for EVs isolation that were posteriorly characterized by flow cytometry and electron microscopy. The uptake and effects of these EVs were analyzed by using in vitro and in vivo models. HK-2 renal tubule cell line was submitted do ATP depletion injury model. Proteomic analyses of these cells treated with EVs after injury were performed by nano-UPLC tandem nano-ESI-HDMSE method. For in vivo analyses, male Wistar rats were submitted to 45 min bilateral ischemia, followed by renal intracapsular administration of ADMSC-EVs within a 72 h reperfusion period. Histological, immunohistochemical and qRT-PCR analysis of these kidneys were performed to evaluate cell death, inflammation and oxidative stress. Kidney function was evaluated by measuring the blood levels of creatinine and urea. RESULTS The results demonstrate that hypoxia increases the ADMSCs capacity to secrete EVs that trigger different energy supply, antiapoptotic, immunomodulatory, angiogenic and anti-oxidative stress responses in renal tissue compared with EVs secreted in normoxia. Proteomic analyses of renal tubule cells treated with EVs from ADMSCs in normoxia and hypoxia give a specific signature of modulated proteins for each type of EVs, indicating regulation of distinct biological processes. CONCLUSION In summary, hypoxia potentially offers an interesting strategy to enhance the properties of EVs in the treatment of acute kidney disease.