Miracle-dz/UniFAR-FTUs huggingface dataset:
The influence of birth season on height: Evidence from Indonesia.

OBJECTIVES Literature on the effect of birth month on height has generally considered regions in temperate climates. However, because many climatic conditions there change with seasons, it is difficult to isolate potential causes. This study estimated the effect of birth month and season on terminal height by analyzing the population of a country with only a few factors driving its climate. MATERIALS AND METHODS The sample was derived from nationally representative data of the Indonesian population. We considered 9,262 men and 10,314 women 20-50 years of age. We applied cosinor analysis to a time series of height by birth month. We then applied a more flexible approach by regressing height on a series of dummy variables for birth month (and, subsequently, season) and birth year fixed effects by sex. RESULTS There was no statistically significant difference in height by birth month. However, although weakly significant, men born in the dry season (June-September) were 2.3 mm shorter than those born in the wet season (the remaining months). The corresponding figure for women was 2.6 mm, a statistically significant difference. DISCUSSION We eliminated some potential factors previously suggested in the literature, including insolation, the position of our planet with respect to the sun, food availability, and maternal workload. We speculate that babies born in the dry season were affected in the third trimester by the high disease burden that characterizes the wet season.
Age-related increase of pulse pressure and gene polymorphisms in essential hypertension: a preliminary study

Genes may modulate the changes of blood pressure (BP) with age; this possibility has never been studied for the age-related increase of pulse pressure (PP), although in older populations, PP is considered the stronger mechanical factor predicting cardiovascular mortality. In humans, the presence of the mutant allele C of the angiotensin II (Ang II) AT1-receptor or of the mutant allele T of the eNOS G298 T gene polymorphisms is associated with enhanced contractile properties of conduit arteries in response to vasoconstrictive agents. In this study, we evaluated, in subjects with untreated essential hypertension, whether the presence of these mutant alleles or their combination might influence the age-related increase of PP. Three main findings emerged from the study and were particularly observed in women: 1) the presence of the C and/or of the T mutant alleles or their combination were associated with a steeper slope of the age versus PP curve, compared with subjects without the mutant allele; 2) the slope was more significantly enhanced when the two mutant alleles were associated in the same genotype; and 3) no comparable age- and gender-related changes in systolic, diastolic or mean BP were found according to this genetic classification. In subjects with essential hypertension, genes may modulate the age-mediated increase of PP. This finding gives new insights in the interactions between genes, mechanical factors and cardiovascular risk.
Identification of Cargo Proteins Specific for the Nucleocytoplasmic Transport Carrier Transportin by Combination of an in Vitro Transport System and Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-based Quantitative Proteomics*

The human importin-β family consists of 21 nucleocytoplasmic transport carrier proteins that carry proteins and RNAs across the nuclear envelope through nuclear pores in specific directions. These transport carriers are responsible for the nucleocytoplasmic transport of thousands of proteins, but the cargo allocation of each carrier, which is necessary information if one wishes to understand the physiological context of transport, is poorly characterized. To address this issue, we developed a high-throughput method to identify the cargoes of transport carriers by applying stable isotope labeling by amino acids in cell culture to construct an in vitro transport system. Our method can be outlined in three steps. (1) Cells are cultured in a medium containing a stable isotope. (2) The cell membranes of the labeled cells are permeabilized, and proteins extracted from unlabeled cells are transported into the nuclei of the permeabilized cells. In this step, the reaction system is first depleted of all importin-β family carriers and then supplemented with a particular importin-β family carrier of interest. (3) Proteins in the nuclei are extracted and analyzed quantitatively via LC-MS/MS. As an important test case, we used this method to identify cargo proteins of transportin, a representative member of the importin-β family. As expected, the identified candidate cargo proteins included previously reported transportin cargoes as well as new potential cargoes, which we corroborated via in vitro binding assays. The identified cargoes are predominately RNA-interacting proteins, affirming that cargoes allotted to the same carrier share functional characteristics. Finally, we found that the transportin cargoes possessed at least two classes of signal sequences: the well characterized PY-nuclear localization signals specific for transportin, and Lys/Arg-rich segments capable of binding to both transportin and importin-β. Thus, our method will be useful for linking a carrier to features shared among its cargoes and to specific nuclear localization signals.
Pandemic Spread of Cholera: Genetic Diversity and Relationships within the Seventh Pandemic Clone of Vibrio cholerae Determined by Amplified Fragment Length Polymorphism

ABSTRACT The seventh cholera pandemic started in 1961 and continues today. A collection of 45 seventh pandemic isolates of V. cholerae sampled over a 33-year period were analyzed by amplified fragment length polymorphism (AFLP) fingerprinting. All but four pairs and one set of three isolates were distinguished. AFLP revealed far more variation than ribotyping, which was until now the most useful method of revealing variation within the pandemic clone. Unfortunately, the ribotype variation observed is mainly due to recombination between the multiple copies of the rrn genes (R. Lan and P. R. Reeves, Microbiology 144:1213–1221, 1998), which makes changes susceptible to repeat occurrences and reversion. This AFLP study shows that particularly for the common ribotypes G and H, such events have indeed occurred. AFLP grouped most of the 45 isolates into two clusters. Cluster I consists mainly of strains from the 1960s and 1970s, while cluster II contains mainly strains from the 1980s and 1990s, revealing a temporal pattern of change in the clone. This is best seen in the relationships of the strains from Africa, which correlate with the epidemiology of epidemics on that continent. The data confirm independent introductions to Africa during the 1970s outbreak and reveal several other African introductions. In the 1991 cholera upsurge, isolates from the Southern and Eastern African epidemic focus are markedly different from those from the West African epidemic focus. An isolate from 1987 in Algeria was identical to the West epidemic isolates, suggesting that the strain was present in Africa at least 3 years before causing large outbreaks. These observations have major implications for our understanding of cholera epidemiology.
Changes in brain activation in stroke patients after mental practice and physical exercise: a functional MRI study

Mental practice is a new rehabilitation method that refers to the mental rehearsal of motor imagery content with the goal of improving motor performance. However, the relationship between activated regions and motor recovery after mental practice training is not well understood. In this study, 15 patients who suffered a first-ever subcortical stroke with neurological deficits affecting the right hand, but no significant cognitive impairment were recruited. 10 patients underwent mental practice combined with physical practice training, and 5 patients only underwent physical practice training. We observed brain activation regions after 4 weeks of training, and explored the correlation of activation changes with functional recovery of the affected hands. The results showed that, after 4 weeks of mental practice combined with physical training, the Fugl-Meyer assessment score for the affected right hand was significantly increased than that after 4 weeks of practice training alone. Functional MRI showed enhanced activation in the left primary somatosensory cortex, attenuated activation intensity in the right primary motor cortex, and enhanced right cerebellar activation observed during the motor imagery task using the affected right hand after mental practice training. The changes in brain cortical activity were related to functional recovery of the hand. Experimental findings indicate that cortical and cerebellar functional reorganization following mental practice contributed to the improvement of hand function.
TOXINOGENY IN CORYNEBACTERIUM DIPHTHERIAE

In 1951, Freeman made the remarkable discovery that nontoxinogenic Corynebacterium diphtheriae became toxinogenic2 after association with the lytic phage B, previously described by Toshach (1950). From lysates of nontoxinogenic, nonlysogenic C. diphtheriae, Freeman and Morse (1951) established, through the isolation of single cells, lysogenic strains which were toxinogenic. The lysogenic clones were resistant to phage B and "carried" a phage which the authors found indistinguishable from phage B. Cultivation of these lysogenic, toxinogenic strains, in antiphage serum, did not eliminate the carried phage. Whereas Freeman's interpretation of his findings was that a spontaneously developing "toxigenic mutant" had been selected by "phage lysis" (Freeman, 1951), the sum of all the evidence to be found in his clearly described observations and experiments was overwhelmingly in favor of phage being the genetic determinant governing toxinogeny. It has since been established that although toxin production is under the genetic control of phage (see Groman, 1955; Barksdale, 1955; Groman and Eaton, 1955) the expression of the tox+ character is possible only in certain strains of corynebacteria (Parsons, 1955; Groman, 1956; Barksdale, 1959). We are interested in when and how the tox+ character becomes expressed in "growing" diphtheria bacilli. It has long been known that C. diphtheriae
Successful Use of Central Venous Catheter as Permanent Hemodialysis Access: 84-Month Follow-Up in Lucania

Cuffed tunneled venous access catheters are commonly used for temporary and permanent access in hemodialysis (HD) patients. These catheters serve an essential role in providing permanent access in subjects in whom all other access options have been exhausted. The predominant complications are catheter thrombosis, catheter fibrin sheating and infection. The aim of this study was to evaluate long-term survival and complications of permanent venous catheters (PVC) placed for the purpose of HD during the period from January 1992 to December 1998, at the Dialysis Units of Lucania (a southern Italian region). A total of 98 PVC were placed in 88 patients during this period. The catheters used were of three types: (a) 72 VasCath Soft Cell catheters (Bard Instrument Company, Toronto, Ont., Canada); (b) 22 PermCath catheters (Quinton Instrument Company, Seattle, Wash., USA), and (c) 4 Tesio catheters (Bellco SpA, Mirandola, Italy). Survival curves of catheters were calculated using the Kaplan-Meier product-limit estimator. The patient survival was 60% at the 78th month. Actually, 52 patients (27 males, 25 females) are still alive: 15 (26.9%) of these patients have diabetes mellitus and 1 has been transplanted. The actuarial survival rate of PVC was 89% in the whole population studied and 82% in subjects alive after 84 months. Twenty-five patients (28.4%) had PVC as the first reliable vascular access. Long-term complications occurred 27 times (1 episode every 44.81 month/patient) as: breakage (3.1%); thrombosis (10.2%); displacement (2.0%); subcutaneous tunnel bleeding (3.1%); inadequate blood flow (7.1%), and infection (10.2%). In conclusion, our data confirm that PVC might represent an effective long-term blood access route for HD. Again, PVC are getting the access of choice for selected patients (i.e., older subjects with cardiovascular diseases and cancer patients) and are enjoying a dramatic increase in use for subjects who are terrified of repetitive venopuncture.
High prevalence of pfmdr1 N86Y and Y184F mutations in Plasmodium falciparum isolates from Bioko island, Equatorial Guinea

Abstract Objective: Drug resistance against Plasmodium falciparum has been recognized as the crucial obstacle to curbing mortality and morbidity from malaria. To investigate the distribution and pattern of multidrug resistance 1 (pfmdr1) gene polymorphisms in P. falciparum, isolates collected from the malaria high-endemic Bioko Island, Equatorial Guinea. Methods: Blood samples were collected from 217 patients with P. falciparum malaria during rainy season in 2012 on Bioko Island. These samples were extracted using Chelex to obtain parasite DNA. Nest-polymerase chain reaction (PCR) and sequencing were employed to detect mutations (N86Y, E130K, Y184F, S1034C, N1042D, V1109I, and D1246Y) and haplotypes in pfmdr1 gene. Results: A total of 151 samples were successfully detected for pfmdr1 mutations from the 217 patients. Pfmdr1 mutations were found in 91·39% (138/151) P. falciparum isolates. However, no mutation at 130 and 1109 was identified from these samples. Four haplotypes coding 86, 184, 1034, 1 042, and 1 246 were found including NYSND, YYSND, NFSND, and YFSND, which accounted for 8·61% (13/151), 2·65% (4/151), 29·80% (45/151), and 58·94% (89/151), respectively. Conclusions: Our results exhibited hypersensitivity to lumefantrine (LU) and mefloquine (MQ) and resistance to chloroquine (CQ) and amodiaquine (AQ) in P. falciparum isolates from Bioko Island. This information will be useful for anti-malarial drug policy in Equatorial Guinea.
Meta-analysis when only the median survival times are known: A comparison with individual patient data results

Background: The hazard ratio (HR) is the most appropriate measure for time to event outcomes such as survival. In systematic reviews, HRs can be calculated either from the raw trial data obtained as part of an individual patient data (IPD) meta-analysis or from the appropriate trial-level summary statistics. However, the information required for the latter are seldom reported in sufficient detail to allow reviewers to calculate HRs. In contrast, the median survival and survival rates at specific time points are frequently presented. We aimed to evaluate retrospectively the performance of meta-analyses using median survival times and survival rates by comparing them with meta-analyses using IPD to calculate HRs. Methods: IPD from thirteen published meta-analyses (MAs) in cancers with high mortality rates were used. Median survival and survival rates were calculated from the IPD rather than taken from publications so that the same trials, patients, and extended follow-up are used in each analysis. Results and Conclusions: We show that using median survival times or survival rates at a particular point in time are not reasonable surrogate measures for meta-analyses of survival outcomes and that, wherever possible, HRs should be calculated. Individual trial publications reporting on time to event outcomes, therefore, should provide more detailed statistical information, preferably logHRs and their variances, or their estimators.
Association of the Vitamin D Receptor Genotype with Bone Metastases in Breast Cancer Patients

This study was designed in order to evaluate specific vitamin D receptor (VDR) genotypes as indicators of the likelihood of developing osseous metastases in breast cancer patients. Therefore, we determined polymorphisms of the VDR gene in a study group comprising 183 breast cancer patients. Specific fragments spanning over intron 8 and exon 9 of the VDR gene were amplified by polymerase chain reaction. The fragments were then incubated with each of the specific endonucleases ApaI, BsmI or TaqI, respectively. The VDR gene polymorphisms were detected by the presence or absence of the particular restriction site using agarose gel electrophoresis. Statistical analyses revealed a significant correlation between both the VDR gene polymorphisms indicated as AA (absence of the ApaI restriction site in both alleles) or TT (absence of the TaqI restriction site in both alleles), respectively, and the occurrence of bone metastases. Patients with the AA genotype have a 1.7-fold increased risk of developing bone metastases, whereas patients with the TT genotype have a 0.5-fold risk. Neither other genotypes nor allelic combinations displayed any further correlation with the clinical stage. The data suggest that the AA genotype of the VDR gene might be useful to identify breast cancer patients with a high probability of forming occult bone metastases who are considered to benefit from an adjuvant bone-protective therapy.
Apoptosis and expression of anti‐ and pro‐apoptotic proteins in peripheral blood mononuclear cells of Fanconi anaemia patients: a study of 73 cases

Abstract: Fanconi anaemia (FA) is a rare genetic disease whose patients have a high predisposition to haematological abnormalities and cancer. Fas expression levels in peripheral blood lymphocytes samples of 73 FA patients were measured to verify if alterations in Fas expression could lead to predisposition/resistance to spontaneous or PHA induced apoptosis, as well as, to reflect some haematological features of this disease. The anti‐ and pro‐apoptotic proteins Bcl‐2 and Bax were also evaluated. FA patients samples could be divided into three different groups based on Fas expression: 20 samples had low, 32 normal and 21 increased Fas levels when compared to 41 control samples. No correlation was found between Fas and Bcl‐2 expression but a good association was obtained with Bax, in the subgroup with increased Fas expression. The best correlation was seen between Bax expression and apoptosis. Out of the 15 samples with high Bax expression, 11 underwent apoptosis whereas only one out of seven samples with low levels of Bax displayed increased induced apoptosis. Most patients with normal haematological features expressed Fas within normal levels. It is difficult to establish, however, if Fas‐expression is involved in the cause or is a consequence of the effects observed.
Migración Rural-Urbana en Bolivia: Ventajas y Desventajas

Este documento discute las ventajas y desventajas de la migracion rural-urbana, y muestra que los costos de una mayor urbanizacion en Bolivia (crimen, polucion, congestion, etc) son mas bien pequenos comparados con los costos encontrados en otros paises latinoamericanos. Los beneficios, por otro lado, pueden ser grandes. Es probable que el incentivar la migracion rural-urbana sea una de las formas mas economicas de reducir la pobreza en Bolivia, ya que la provision de servicios basicos tales como la electricidad, el agua potable, escuelas y servicios de salud para la poblacion es mucho mas economica cuando las personas estan agrupadas en poblados o ciudades. Adicionalmente, las economias de escala en las ciudades generan oportunidades economicas e incrementan los ingresos de la poblacion.
Perceptual Criteria and Attributes Used for Evaluation of Clothing by Women Using Wheelchairs

The apparel and clothing attributes important to persons with disabilities are poorly understood. The purpose of this study was to identify apparel attributes of persona] value to persons using a wheelchair and used to evaluate clothing. Four focus group interviews on three categories of clothing, formal wear, casual wear, and undergarments, were conducted with 19 female wheelchair users. Participants discussed clothing generally and after viewing garments designed especially for people with disabilities. Analysis of the content of the interviews yielded 49 phrases or words that could be categorized into Physical Appearance, Physical Performance, Expressiveness, and Extrinsic Attributes. Participants were concerned most about Physical Appearance for which criteria are observable attributes of the garment. Attributes of value in order of importance are styling of garment, fabric, garment construction, and color. The Physical Performance criteria include in order of attention benefits of physical aspects such as convenience, performance of garment, fabric, and effect of care. Expressiveness criteria evoked participants' feelings and reaction to use. Extrinsic Attribute criteria are those used to evaluate and nuke purchase decisions and are of less importance. These 19 persons with disability value casual clothing which had regular features. Results describe useful information on the criteria disabled persons use to evaluate clothing for use.
Food prices and obesity: evidence and policy implications for taxes and subsidies.

CONTEXT Pricing policies have been posited as potential policy instruments to address the increasing prevalence of obesity. This article examines whether altering the cost of unhealthy, energy-dense foods, compared with healthy, less-dense foods through the use of fiscal pricing (tax or subsidy) policy instruments would, in fact, change food consumption patterns and overall diet enough to significantly reduce individuals' weight outcomes. METHODS This article examined empirical evidence regarding the food and restaurant price sensitivity of weight outcomes based on a literature search to identify peer-reviewed English-language articles published between 1990 and 2008. Studies were identified from the Medline, PubMed, Econlit, and PAIS databases. The fifteen search combinations used the terms obesity, body mass index, and BMI each in combination with the terms price, prices, tax, taxation, and subsidy. FINDINGS The studies reviewed showed that when statistically significant associations were found between food and restaurant prices (taxes) and weight outcomes, the effects were generally small in magnitude, although in some cases they were larger for low-socioeconomic status (SES) populations and for those at risk for overweight or obesity. CONCLUSIONS The limited existing evidence suggests that small taxes or subsidies are not likely to produce significant changes in BMI or obesity prevalence but that nontrivial pricing interventions may have some measurable effects on Americans' weight outcomes, particularly for children and adolescents, low-SES populations, and those most at risk for overweight. Additional research is needed to be able to draw strong policy conclusions regarding the effectiveness of fiscal-pricing interventions aimed at reducing obesity.
Implikasi Undang-Undang Nomor 30 Tahun 2014 tentang Administrasi Pemerintahan terhadap Fungsi Peradilan Tata Usaha Negara

Penelitian ini bertujuan untuk menjawab permasalahan tentang: 1) Bagaimanakah implikasi UU Administrasi Pemerintahan terhadap fungsi PTUN, apakah keberadaan UU Administrasi Pemerintahan memperlemah atau memperkuat fungsi PTUN sebagai lembaga pengawasan? 2) Apakah perlu dilakukan upaya penguatan fungsi PTUN seiring dengan meluasnya kompetensi absolut PTUN setelah berlakunya UU Administrasi Pemerintahan? Penelitian ini merupakan penelitian hukum normatif, yaitu menganalisis bahan-bahan hukum primer, sekunder, dan tersier. Bahan hukum dianalisis secara yuridis kualitatif. Hasil penelitian menyimpulkan bahwa: 1) Perluasan kompetensi absolut PTUN dapat menimbulkan implikasi memperlemah atau menguatkan fungsi PTUN bergantung pada integritas SDM termasuk hakim; dan 2) Perlu penguatan fungsi PTUN seiring dengan meluasnya komptensi PTUN berdasarkan UU Administrasi Pemerintahan yaitu dengan: a) segera dilakukan perubahan UU PTUN menyesuaikan dengan UU Administrasi Pemerintahan dan meningkatkan daya paksa pelaksanaan putusan dengan membentuk lembaga eksekusi untuk melaksanakan putusan PTUN;Â b) meningkatkan independensi hakim PTUN dalam memeriksa, memutus dan menyelesaikan sengketa atau permohonan.