Miracle-dz/UniFAR-FTUs huggingface dataset:
Abstract #4406: Hyperglycemia regulates RUNX2 activation and cellular wound healing through the aldose reductase polyol pathway

Cancer cells are known to rely on glucose for energy in a process of aerobic glycolysis called the Warburg effect that is believed to contribute to tumor growth, survival, and metastasis. The IGFR/PI3K/Akt/mTOR pathway is important in this process because it activates Glut1,2 transporter-mediated glucose uptake and apoptotic resistance in transformed cells. Components of the microenvironment, such as tumor vessels, may also depend on glycolysis to provide the necessary nutrients and energy required for angiogenesis. Therefore, vascular endothelial cells (ECs) could be sensitive to the same anti-glycolytic strategies proposed for tumor cells. The RUNX2 transcription factor, which is expressed in metastatic tumor cells and ECs, promotes vascular EC migration, proliferation, and angiogenesis in response to IGF1/IGFR activation. Here we show that physiological levels of glucose (euglycemia) increase RUNX2 DNA binding and transcriptional activity, while hyperglycemia (HG) does not. The aldose reductase (AR) polyol pathway has been shown to mediate vascular damage in response to HG, but may also neutralize toxic aldehyde byproducts of increased metabolism and contribute to drug resistance in cancer cells. Pharmacologic or genetic inhibition of AR in ECs reversed the HG suppression of RUNX2. IGF-1 secretion and IGFR phosphorylation by autocrine IGF-1 occurred equally well in euglycemic or HG conditions, suggesting that reduced RUNX2 activity in HG was not due to altered IGF-1/IGFR activation. AR also negatively regulated RUNX2-dependent vascular remodeling in an EC wounded monolayer assay, which was reversed by specific AR inhibition in HG. These current data suggest that RUNX2 mediates normal vessel function at physiological levels of glucose while HG conditions increase AR, inhibit RUNX2 activation, and suppress vascular remodeling; therefore, drugs that inhibit the AR pathway and enhance RUNX2 expression might normalize tumor vasculature, allowing chemotherapeutic delivery and better therapeutic response. Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 4406.
Localization of the streptococcal C5a peptidase to the surface of group A streptococci

Immunofluorescent staining was used to determine that the streptococcal C5a peptidase (SCP) exists as a cell surface antigen on group A streptococci. The ability of hyperimmune serum to neutralize cell-associated SCP activity provided further evidence for the location of SCP. Quantification of SCP during growth in vitro by indirect enzyme-linked immunosorbent assay showed that approximately 90% of the measurable antigen is cell bound.
Attachment theory in adult psychiatry. Part 1: Conceptualisations, measurement and clinical research findings

Since its original conception by Bowlby to explain an important evolutionary function of the child–caregiver relationship, attachment theory has been supported by much empirical research in a variety of settings. However, although attachment theory began as a clinical enterprise, its wider application to the everyday clinical understanding of adult mental health problems has lagged behind the available research. The theory can afford valuable insight not only into the developmental nature of common psychiatric disorders, but also into the development of the therapeutic relationship. This article gives an overview of (a) the current conceptualisations of attachment and the measurement of attachment for clinical research purposes and (b) the application of attachment theory to different psychopathologies. The importance of attachment to the therapeutic relationship will be discussed in a future article.
Multimodal Feedback and Interaction Techniques for Physically Based and Large Virtual Environments. (Retour Multimodal et Techniques d'Interaction pour des Environnements Virtuels Basés Physique et Larges)

Virtual Reality allows the simulation and interaction with Virtual Environments (VE) through different sensory modalities. However, interacting with complex physically based VE, such as non-rigid or large environments, presents many challenges in terms of interaction and sensory feedback. The first part of this Ph.D. thesis addresses haptic and multimodal feedback issues during manipulation of non-rigid media. We first present a novel approach for 6 degrees of freedom haptic interaction with fluids, allowing the generation of force feedback from viscous fluids through arbitrary-shaped rigid bodies. This approach is extended by including the haptic interaction with deformable bodies, thus allowing a unified haptic interaction with the different states of matter. A perceptual experiment showed that users could efficiently identify the different states through the haptic modality alone. Then, we introduce a novel vibrotactile fluid rendering model, leveraging previous knowledge on fluid sound synthesis. Through this approach, we allow the interaction with fluids with multimodal feedback, through vibrotactile, kinesthetic, acoustic and visual sensory channels. The second part of this Ph.D. thesis addresses interaction issues during walking navigation of large VE. Since the VE is often larger than the available real workspace, we introduce a novel navigation metaphor that informs users about the real physical boundaries. Using hybrid position/rate control, this technique provides a simple and intuitive metaphor for a navigation safe from collisions and breaks of immersion. Other workspaces, such as CAVE-like environments, present rotation boundaries due to missing screens. Thus, we present three novel metaphors dealing with these additional boundaries. Overall, the evaluation of these navigation techniques showed that they efficiently fulfilled their objectives while being highly appreciated by users.
Spastic paraparesis, cerebellar ataxia, and intention tremor: a severe variant of FXTAS?

Fragile X associated tremor/ataxia syndrome (FXTAS) is a recently identified neurodegenerative disorder affecting older adult males with pre-mutation alleles of the fragile X mental retardation 1 ( FMR1 ) gene.1–3 These male carriers, in their fifties and older, develop progressive intention tremor, cerebellar ataxia, progressive cognitive difficulties, and variable features including peripheral neuropathy, lower limb proximal muscle weakness, and autonomic dysfunction. Radiological signs include global brain atrophy and white matter disease characterised by hyperintensities in the middle cerebellar peduncles (MCP) on T2 sequence with scattered periventricular disease, corresponding to spongiform changes and demyelination on neuropathological studies.2 Here we report three pre-mutation carriers (two sisters and one sporadic case) presenting with spastic paraparesis, cerebellar ataxia, and intention tremor, and having neuroimaging features suggestive of a severe variant of FXTAS. A random X inactivation pattern was found in the two affected sisters. The third female sibling was unaffected and her inactivation pattern was significantly skewed. This suggests that the X inactivation ratio may be responsible for the incomplete penetrance of the neurological symptoms in this family. ### Case 1 (II-2) The first sister developed spasticity and balance difficulty in the third decade of her life. During this time, she underwent neurological evaluation. She was well oriented, with intact speech and cognitive function. Examination of the lower extremity showed paraparesis, mild spasticity, and positive Babinski. The upper extremities showed diminished co-ordination and intention tremor. EEG, ECG fundoscopy, electromyography (EMG), and nerve conduction studies were normal. Cerebrospinal fluid (CSF) was normal, with absence of oligoclonal bands. Muscle biopsy showed a chronic neurogenic process. She had anxiety and mild depression. ### Case 2 (II-3) The other sister gradually developed spasticity with dragging of the toes while walking, lower limb weakness, and difficulty with balance. Neurological evaluation at 35 years of age showed normal cognitive functions. Examination of the lower extremities, showed paraparesis, …
The glucose paradox. Is glucose a substrate for liver metabolism?

Dans la plupart des conditions seulement avec des charges excessives de glucose rarement rencontrees chez l'homme et le rat, le glucose serait capte efficacement de maniere directe par le foie. Il semble plutot que dans la phase post-grandiale immediate les glucides alimentaires soient convertis en glycogene et graisses hepatiques par un mecanisme indirect mettant en jeu la sequence: glucose→unite C 3 →glycogene et lipides. Le C 3 peut etre de l'acide lactique (et probablement de l'alanine)
Enhancing innovation through learning: the roles of market orientation and interorganisational knowledge stores

Drawing on the organisational learning theory, this study regards market orientation as a market-based learning and seeks to advance insight into how market orientation and interorganisational knowledge stores together affect different types of innovation. Our findings from a sample of 246 manufacturing firms provide supporting evidence for the positive effect of market orientation on both explorative and exploitative innovation. The effect of market orientation on innovation gets amplified when firms possess large amount of interorganisational knowledge, demonstrating the synergistic effect produced by different types of learning which is less known. This study, therefore, sheds light on the under-researched area of literature surrounding the interaction between different learning activities and the roles of knowledge from different sources on firm innovation.
The effects of black garlic on the working memory and pyramidal cell number of medial prefrontal cortex of rats exposed to monosodium glutamate

Abstract Monosodium glutamate-induced exitotoxicity causes oxidative stress in many brain areas, including the medial prefrontal cortex. The ethanolic garlic (Allium sativum) extract is considered as a neuroprotective substance. The present study aimed at investigating the effects of the ethanolic fermented garlic extract on the working memory and the pyramidal cell number of the medial prefrontal cortex of adolescent male Wistar rats exposed to monosodium glutamate (MSG). Twenty-five rats were randomly divided into five groups. The C– group was given 0.9% NaCl solution. The C + group was given 2 mg/g bw of MSG. The T1, T2, and T3 groups were given MSG and garlic extract (0.0125, 0.025, and 0.05 mg/g bw, respectively). All treatments were conducted for 10 days. The working memory capability of the rats was measured using Y-maze test. The total number of pyramidal cells of the medial prefrontal cortex was estimated using physical fractionator method. The working memory performances of the T1, T2, and T3 groups were significantly better than that of the C + group. There were no significant differences between groups in the estimated total number of pyramidal cell of medial prefrontal cortex. The MSG may not cause the death of neurons, but it may modify neuronal architectures that are sufficient to disrupt memory functions. Black garlic may play a role as an antioxidant agent that prevents the prefrontal cortex from glutamate-induced oxidative stress. It is concluded that the ethanolic fermented garlic extract prevented the working memory impairment following MSG administration.
Relationship between N-linked oligosaccharide chains of human serum immunoglobulin G and serum tumor markers with non-small cell lung cancer progression.

BACKGROUND It has been demonstrated that increased expression of the sialyl Le(x) sugar chains on cancer cells influences cellular adhesion to vascular-endothelial cells. Therefore, it was thought that alterations in the sugar chain structure of E-cadherin, a calcium dependent adhesion molecule, influence the metastasis of cancer cells. MATERIALS AND METHODS In this study, N-linked oligosaccharides of human serum immunoglobulin G (IgG) were analyzed in 12 patients with non-small cell lung cancer (NSCLC) (6 localized cancer: 3 adenocarcinomas and 3 squamous cell carcinomas; 6 metastatic cancer: 3 adenocarcinomas and 3 squamous cell carcinomas) and 10 healthy controls using fluorophore-associated carbohydrate electrophoresis (FACE). The relationship between changes in sugar chain structure and serum concentrations of carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA21-1) were evaluated. CEA levels in the sera were determined using an enzyme immunoassay, and CYFRA21-1 levels were determined using an enzyme chemiluminescent immunoassay. RESULTS Fri (monogalactosyl IgG oligosaccharides) and Fr2 (digalactosyl IgG oligosaccharides) decreased, while Fr4 (agalactosyl IgG oligosaccharides) significantly increased (p < 0.01-0.05) with NSCLC progression. The Fr4/Fr1+Fr2 ratio increased with NSCLC progression, and the ratios in localized and metastatic NSCLC were significantly higher than in healthy controls (p < 0.01 and p < 0.01, respectively). There was a strong correlation between serum CEA levels and Fr4 (r = 0.91) and a significant correlation between serum CEA levels and the Fr4/Fr1+Fr2 ratio (r = 0.83, p < 0.05) in patients with lung adenocarcinoma. There was a significant correlation between serum CYFRA21-1 levels and Fr4 (r = 0.88, p < 0.001) and a positive correlation between serum CYFRA21-1 levels and the Fr4/Fr1+Fr2 ratio (r = 0.38) in patients with lung squamous cell carcinoma. CONCLUSION The analysis of serum IgG N-linked oligosaccharide chain structures by FACE may be an auxiliary indicator of serum tumor markers for monitoring NSCLC progression.
Gutka syndrome and oral submucous fibrosis: few observations.

The proposed new terminology of gutka or areca nut chewer’s syndrome to the already existing wellknown premalignant condition—oral submucous fibrosis (OSF)—is interesting as a synonym. It is understood by the scientific community that areca nut and its various commercial preparations could lead to OSF,[2,3] presenting as a wide clinical spectrum as narrated by the author. Traditionally, pan chewing is a habit, which was prevalent as a cultural or social habit over hundreds of years in different parts of the Indian subcontinent and Asian countries. [4] However, the newer commercial preparations, which are widely used by the younger age group and among certain occupational sectors, are alarming.
Hybrid Software Defined Networking Controller

There exists some common bottlenecks among the many available Software Defined Networking (SDN) controllers. Of these, the current analysis uses the Floodlight software controller to identify bottlenecks. The analysis has implemented these bottlenecks in a Field Programmable Gate Array (FPGA) in order to assess its feasibility. Thereafter, a Hybrid SDN controller has been developed. Generally Hybrid SDN refers to a networking approach where traditional networking and SDN protocols are used and operated in the same environment. Hybrid in this paper context refers to a controller which is developed both on software and hardware platforms in order to achieve a higher scalability which is a current issue existing in software controller, and thereby overcoming the identified bottlenecks. The analysis has offloaded computational intensive models to an FPGA and tested for performance. These performance figures have then been compared against the performance figures as of a stand-alone software controller.
Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair

We describe a transposon insertion that reduces the efficiency of homologous recombination and DNA repair in Escherichia coli. The insertion, rec-258, was located between pyrE and dgo at min 82.1 on the current linkage map. On the basis of linkage to pyrE and complementation studies with the cloned rec+ gene, rec-258 was identified as an allele of the recG locus first reported by Storm et al. (P. K. Storm, W. P. M. Hoekstra, P. G. De Haan, and C. Verhoef, Mutat. Res. 13:9-17, 1971). The recG258 mutation confers sensitivity to mitomycin C and UV light and a 3- to 10-fold deficiency in conjugational recombination in wild-type, recB recC sbcA, and recB recC sbcB sbcC genetic backgrounds. It does not appear to affect plasmid recombination in the wild-type. A recG258 single mutant is also sensitive to ionizing radiation. The SOS response is induced normally, although the basal level of expression is elevated two- to threefold. Further genetic studies revealed that recB recG and recG recJ double mutants are much more sensitive to UV light than the respective single mutants in each case. However, no synergistic interactions were discovered between recG258 and mutations in recF, recN, or recQ. It is concluded that recG does not fall within any of the accepted groups of genes that affect recombination and DNA repair.
Antiproliferative effect of pheophorbide a–mediated photodynamic therapy and its synergistic effect with doxorubicin on multiple drug-resistant uterine sarcoma cell MES-SA/Dx5

Abstract Prolonged cancer chemotherapy is associated with the development of multidrug resistance (MDR), which is a major cause of treatment failure. Photodynamic therapy (PDT) has been applied as anticancer therapy and a means of circumventing MDR. The antiproliferative effect of pheophorbide a–mediated photodynamic therapy (Pa-PDT) has been demonstrated in several human cancer cell lines, including the uterine sarcoma cell line, MES-SA. This study set out to evaluate, first, the therapeutic potential of Pa-PDT on MES-SA/Dx5 uterine sarcoma cells and, subsequently, the effectiveness of combination therapy using Pa-PDT with doxorubicin (Dox). Our results showed that Pa-PDT was able to circumvent MDR in the P-glycoprotein (P-gp) overexpressing human uterine sarcoma cell line, MES-SA/Dx5. Intracellular accumulation of Pa and Pa-PDT-induced cell death was not abrogated by MDR phenotype, when compared to the parental cell line, MES-SA. Combined therapy using Pa-PDT and Dox, a common chemotherapeutic drug, was found to be synergistic in the cell line, MES-SA/Dx5. Both activity and expression of MDR1 and P-gp were reduced by Pa-PDT treatment and such reductions were attenuated by α-tocopherol, the scavenger of reactive oxygen species (ROS), suggesting that the effect of Pa-PDT was mediated by the generation of intracellular ROS. In conclusion, our findings demonstrated the therapeutic potential of Pa-PDT alone or in combination with Dox in combating multidrug-resistant malignancies.
Adrenocortical neoplasms: role of prognostic markers MIB-1, P53, and RB.

Differentiation between benign and malignant adrenocortical neoplasms is made using a combination of clinical and pathologic parameters. Despite these parameters, it is still difficult to predict the biologic potential of some tumors. Forty adrenocortical lesions, including 10 hyperplasias, 10 adenomas, 12 carcinomas and eight metastatic/recurrent adrenocortical carcinomas were studied for the expression of MiB-1, p53, and the retinoblastoma gene product (RB) utilizing immunohistochemical techniques. The mean tumor proliferating fraction (TPF), expressed as the number of MiB-1-positive nuclei per 1,000 tumor cells, was 14.9 in adenomas, 31.5 in hyperplasias, 208.1 in carcinomas and 166.1 in recurrent or metastatic disease. None of the 20 benign lesions had a TPF of > 80, and only one of the 20 malignancies had a TPF of < 80. Nine of the 20 carcinomas were positive for p53. None of the benign lesions were p53 positive. Thirty-nine cases, including benign and malignant ones, were RB positive, and one was uninterpretable. We conclude that prognostic markers can be of great assistance in recognizing adrenocortical carcinomas. High TPF (> 80), as measured by staining with MiB-1, and positive p53 strongly correlate with malignant behavior and therefore may be useful in distinguishing benign from malignant adrenal lesions. Staining for RB does not appear to be a helpful technique.
Socially Desirable Responding in Chinese University Students: Denial and Enhancement?

This study examined the Balanced Inventory of Desirable Responding (BIDR) with one-, two-, three-, and four-dimensional models and tested the BIDR's discriminant validity with personality variables. A confirmatory and exploratory factor analysis of responses from 600 Chinese university students (314 men, 282 women, 4 missing; M age = 20.0 yr.) provided results indicating that the four-factor model fit the data best; i.e., self-deception and impression management split into denial and enhancement. The Denial and Enhancement subscales with personality variables show significant differences, confirming the four-factor model. The cultural differences as a possible reason for the split were discussed.
Hepatoprotective and Antioxidant Effects of Silybum Marianum Plant against Hepatotoxicity Induced by Carbon Tetrachloride in Rats

The acute toxicity of ethanolic extract of Silybum marianum (SM) plant seeds and its effect on body weight were studied in rats. In vitro determination of the antioxidant activity of SM extract using 1, 1diphenyl 2-picryl hydrazyl (DPPH) radical was carried out. The hepatoprotective and in vivo antioxidant effects of SM extract were evaluated in CCL4 intoxicated rats. Estimation of serum liver enzymes (ALT, AST and ALP), antioxidant enzymes (SOD, GPx and CAT) in hepatic tissue and liver histopathology were the parameters used in the study. The results showed that no mortalities occur when SM extract was orally given to rats in graded doses up to 8.0 g b.wt. kg. SM extract induced a significant in vitro antioxidant activity when compared to standard ascorbic acid. Pretreatment of CCL4–intoxicated rats with SM extract (200 and 400 mg/kg b.wt./day) for 8 weeks significantly (P< 0.05) decreased the elevated serum liver enzymes and increased the activity of antioxidant enzymes in liver homogenate when compared to the CCL4-poisoned group. These biochemical findings were accompanied by amelioration of hepatic degenerative changes (vacuolar degeneration and necrosis) induced by CCL4. The results proved the protective effect of Silybum marianum on liver cells. The protective effect of SM extract may be attributed to the antioxidant effect flavonoids present in this plant. It could be concluded that Silybium marianum plant have high safety, hepatoprotective effect and antioxidant activity in rats. The study recommends that intake of Silybium marianum plant may be beneficial for patients who suffer from liver diseases associated with oxidative stress. [Nevien I. Soufy. Hepatoprotective and Antioxidant Effects of Silybum Marianum Plant against Hepatotoxicity Induced by Carbon Tetrachloride in Rats. Journal of American Science. 2012; 8(4): 479-486]. (ISSN: 1545-1003). http://www.americanscience. org. 64
Serological studies of measles and subacute sclerosing panencephalitis

THE RELATION between measles virus and subacute sclerosing panencephalitis ( SSPE) has been suggested by the findings in brain tissue specimens from patients with this disease. These findings have included Type A inclusions,l myxovirus-like particles,and specific fluorescence with measles antibody.6. 7 In addition, several groups have reported high measles complement fixing (CF) and hemagglutination inhibition (HI) titers in the sera and spinal fluid of affected individuals. "s For some patients rising titers were noted during the course of the illness, while in most instances there was no change in antibody. In one case when the patient recovered, there was a loss of detectable CF antibody and a persistence of HI titer. * The present studies were designed to further define the serological findings with measles and other viruses in SSPE.