Miracle-dz/UniFAR-FTUs huggingface dataset:
Nano hydroxyapatite‐blasted titanium surface affects pre‐osteoblast morphology by modulating critical intracellular pathways

Although, intracellular signaling pathways are proposed to predict the quality of cell‐surface relationship, this study addressed pre‐osteoblast behavior in response to nano hydroxyapatite (HA)‐blasted titanium (Ti) surface by exploring critical intracellular pathways and pre‐osteoblast morphological change. Physicochemical properties were evaluated by atomic force microscopy (AFM) and wettability considering water contact angle of three differently texturized Ti surfaces: Machined (Mac), Dual acid‐etching (DAE), and nano hydroxyapatite‐blasted (nHA). The results revealed critical differences in surface topography, impacting the water contact angle and later the osteoblast performance. In order to evaluate the effect of those topographical characteristics on biological responses, we have seeded pre‐osteoblast cells on the Ti discs for up to 4 h and subjected the cultures to biological analysis. First, we have observed pre‐osteoblasts morphological changes resulting from the interaction with the Ti texturized surfaces whereas the cells cultured on nHA presented a more advanced spreading process when compared with the cells cultured on the other surfaces. These results argued us for analyzing the molecular machinery and thus, we have shown that nHA promoted a lower Bax/Bcl2 ratio, suggesting an interesting anti‐apoptotic effect, maybe explained by the fact that HA is a natural element present in bone composition. Thereafter, we investigated the potential effect of those surfaces on promoting pre‐osteoblast adhesion and survival signaling by performing crystal violet and immunoblotting approaches, respectively. Our results showed that nHA promoted a higher pre‐osteoblast adhesion supported by up‐modulating FAK and Src activations, both signaling transducers involved during eukaryotic cell adhesion. Also, we have shown Ras‐Erk stimulation by the all evaluated surfaces. Finally, we showed that all Ti‐texturing surfaces were able to promote osteoblast differentiation up to 10 days, when alkaline phosphatase (ALP) activity and osteogenic transcription factors were up‐modulated. Altogether, our results showed for the first time that nano hydroxyapatite‐blasted titanium surface promotes crucial intracellular signaling network responsible for cell adapting on the Ti‐surface.Biotechnol. Bioeng. 2017;114: 1888–1898. © 2017 Wiley Periodicals, Inc.
Rapid increases in mercury concentrations in the eggs of mallards fed methylmercury

To determine how quickly breeding birds would have to feed in a mercury‐contaminated area before harmful concentrations of mercury, as methylmercury, built up in their eggs, we fed female mallards (Anas platyrhynchos) a control diet or diets containing 0.5, 1, 2, 4, or 8 μg/g mercury (on what was close to a dry weight basis) as methylmercury chloride for 23 d. After 18 d on their respective mercury diets, the eggs of mallards fed 0.5, 1, 2, 4, or 8 μg/g mercury contained 97.8, 86.0, 89.9, 88.9, and 85.9%, respectively, of the peak concentrations reached after 23 d. Depending on the dietary concentration of mercury, no more than approximately a week may be required for harmful concentrations (0.5–0.8 μg/g, wet weight) to be excreted into eggs.
Making Sense of the History of Information Systems Research 1975-1999: A View of Highly Cited Papers

In this paper, I analyze the history of IS research through the lens of 409 highly cited papers (i.e., papers with at least 100 Reuters Thompson Web of Science citations) published between 1975 and 1999. I focus on 1) what these highly cited papers are, 2) what they study, 3) who their authors are, and 4) where they were published.
A Systematic Literature Review and Research Agenda for Organ Donation Decision Communication

Background: This study systematically located and appraised peer-reviewed evidence for the efficacy of strategies to increase organ donation decision communication among adults including an assessment of study quality to guide future research in this field. There is little room to move in strengthening unanimously positive public attitudes toward organ donation. Consequently, researchers have called for a focus on organ donation decision communication to understand modifiable factors to increase organ donation rates. Methods: Multiple databases were searched during September 2015, and 44 studies were selected for inclusion. Data concerning participants, design, and outcomes were extracted. Studies were rated for quality and levels of evidence. Findings: Although not amenable to meta-analysis, the literature indicates that approximately 50% of adults who are willing to become an organ donor have discussed this decision with family. The majority of research was conducted in a Western context with an overrepresentation of students. Strategies to increase communication include education, motivation, input from lived experience, efforts to address salient audience beliefs, and scheduled reminders or prompts. Intentions and willingness to discuss organ donation were consistently positively related to discussion behavior; however, formative research and experimental studies testing theoretically driven interventions were scarce. Conclusions: There is mixed evidence for the role of demographic and attitudinal characteristics in the success of organ donation communication interventions. Additional theoretically based research is recommended to establish boundary conditions and validate strategies to increase organ donation decision communication among adults.
Prostate volume and cancer in screening programs

Prostate cancer screening is studied in a randomized trial in Antwerp, Belgium. The case group receives three screening tests (DRE, TRUS, and PSA). Intermediate evaluation shows that only 1/3 of the biopsy results is positive (35/125). The proposed analysis identifies variables that determine the biopsy outcome.
An inductorless noise-cancelling CMOS LNA using wideband linearization technique

An inductorless noise-cancelling CMOS low-noise amplifier (LNA) with wideband linearization technique is proposed. The complementary configuration by stacked NMOS/PMOS is employed to compensate second-order nonlinearity of the circuit. The third-order distortion of the auxiliary stage is also mitigated by that of the weak inversion transistors in the main path. The bias and scaling size combined by digital control words is further tuned to obtain enhanced linearity over the desired band. Implemented in a 0.18-μm CMOS process, simulated results show that the proposed LNA provides a voltage gain of 16.1 dB and a NF of 2.8∼3.4 dB from 0.1 to 1.4 GHz. The IIP3 and IIP2 of 13∼18.9 and 24∼40 dBm are obtained, respectively. The circuit core consumes 19 mW from a 1.8 V supply.
Global economic burden of schizophrenia: letter in response

and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). Neuropsychiatric Disease and Treatment 2016:12 3069–3072 Neuropsychiatric Disease and Treatment Dovepress
Mantle‐zone lymphoma: A follicular variant of intermediate lymphocytic lymphoma

A clinicopathologic analysis of 12 cases of a morphologically distinctive non‐Hodgkin's malignant lymphoma which appeared to arise from the mantle zones of secondary lymphoid follicles is presented. In lymph node and spleen sections, atypical small lymphoid cells with slightly irregular and indented nuclear contours proliferated as wide mantles around non‐neoplastic‐appearing germinal centers. Surface marker analysis performed on lymph nodes revealed a monoclonal B‐cell proliferation in two cases. Clinically, the patients usually had advanced disease (Stage III or IV) associated with splenomegaly. This lymphoma appears to be relatively low‐grade, since nine of 12 patients are alive with a median follow‐up period of 28.5 months. We believe that this “mantle‐zone lymphoma” represents a nodular or follicular variant of intermediate lymphocytic lymphoma.
Voluntary adopters versus forced adopters: integrating the diffusion of innovation theory and the technology acceptance model to study intra-organizational adoption

This study extends diffusion research to the intra-organizational level and integrates the classic diffusion of innovation theory (DIT) with the relatively new technology acceptance model (TAM) to empirically explore Chinese journalists' adoption of the internet. It makes a theoretical contribution by proposing four adoption categories — voluntary adopters, forced adopters, resistant non-adopters, and dormant non-adopters — according to the voluntariness of organizational members' innovation decision-making. Based on data from a nationwide survey of 813 journalists in China, this study demonstrates that the DIT and TAM are respectively related to voluntary and forced adoption of the internet.Young, male journalists who perceive the internet positively (i.e., relative advantage and ease of use) and think it to be popular in society are most likely to be voluntary adopters. High-ranking journalists who believe the internet can enhance their job performance and who work in large and technologically sophisticated organizations are most likely to be forced adopters.
C‐peptide modifies leptin and visfatin secretion in human adipose tissue

The effects of C‐peptide on adipose tissue, an organ involved in the development of obesity and insulin resistance, are not yet well known. The aim of this study was to determine whether C‐peptide could be involved in the regulation of the adipocytokine synthesis in human visceral adipose tissue.
Orally Active Osteoanabolic Agent GTDF Binds to Adiponectin Receptors, With a Preference for AdipoR1, Induces Adiponectin-Associated Signaling, and Improves Metabolic Health in a Rodent Model of Diabetes

Adiponectin is an adipocytokine that signals through plasma membrane–bound adiponectin receptors 1 and 2 (AdipoR1 and -2). Plasma adiponectin depletion is associated with type 2 diabetes, obesity, and cardiovascular diseases. Adiponectin therapy, however, is yet unavailable owing to its large size, complex multimerization, and functional differences of the multimers. We report discovery and characterization of 6-C-β-d-glucopyranosyl-(2S,3S)-(+)-5,7,3′,4′-tetrahydroxydihydroflavonol (GTDF) as an orally active adiponectin mimetic. GTDF interacted with both AdipoRs, with a preference for AdipoR1. It induced adiponectin-associated signaling and enhanced glucose uptake and fatty acid oxidation in vitro, which were augmented or abolished by AdipoR1 overexpression or silencing, respectively. GTDF improved metabolic health, characterized by elevated glucose clearance, β-cell survival, reduced steatohepatitis, browning of white adipose tissue, and improved lipid profile in an AdipoR1-expressing but not an AdipoR1-depleted strain of diabetic mice. The discovery of GTDF as an adiponectin mimetic provides a promising therapeutic tool for the treatment of metabolic diseases.
Morphology-Based Phylogeny and Biogeography of Wockia (Lepidoptera: Urodidae) with Description of a New Species from Japan and South Korea

Wockia, one of the six genera within the lepidopteran family Urodidae, currently includes nine species distributed in Holarctic, Oriental, and Neotropical regions. A new species of the genus, W. magna sp. nov., is described from Japan and South Korea. This is the first record of Urodidae from Japan. A cladistic analysis was constructed based on 27 morphological characters from five ingroup species and two outgroup taxa. A single most parsimonious tree was found (length = 38, CI = 71, RI = 70). The resulting tree failed to recover the monophyly of Wockia chewbacca and a clade Wockia sensu stricto, including four congeners from North America, temperate East Asia and Southeast Asia. Wockia sensu stricto was resolved as monophyletic and was divided into two subgroups, one including W. asperipunctella and W. magna, and the other including W. koreana and W. variata. The sister-group relationship of W. asperipunctella and W. magna was moderately supported. Synapomorphies of Wockia sensu stricto are provided from the resulting phylogeny. Systematic definitions of Wockia and other allied genera are revised. Optimal ancestral area reconstruction implemented in DIVA resulted in different hypotheses for Wockia, depending on how to constrain the maximum number of areas. The maximum area number set as two yielded a more likely scenario suggesting that Wockia sensu stricto originated in temperate East Asia and then dispersed into the Oriental region, North America, and Europe. A working hypothesis and other equally possible alternative explanations for the biogeography of Wockia are provided.
Amoxicillin for clinically unsuspected pneumonia in primary care: subgroup analysis

Clinically unsuspected pneumonia does benefit from antibiotic treatment in terms of duration and severity of symptoms http://ow.ly/T46up Antibiotic treatment is recommended for pneumonia [1, 2]. However, some studies have suggested that the milder spectrum of pneumonia in primary care does not have important adverse consequences if missed and, therefore, managed without antibiotics [3–5]. Better insight in the effects of antibiotics for pneumonia in primary care could improve patient information and management.
Mutagenesis of the phosphate‐binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates

Narrow substrate specificities often limit the use of enzymes in biocatalysis. To further the development of Escherichia coli 2‐keto‐3‐deoxy‐6‐phosphogluconate (KDPG) aldolase as a biocatalyst, the molecular determinants of substrate specificity were probed by mutagenesis. Our data demonstrate that S184 is located in the substrate‐binding pocket and interacts with the phosphate moiety of KDPG, providing biochemical support for the binding model proposed on the basis of crystallographic data. An analysis of the substrate selectivity of the mutant enzymes indicates that alterations to the phosphate‐binding site of KDPG aldolase changes the substrate selectivity. We report mutations that enhance catalysis of aldol cleavage of substrates lacking a phosphate moiety and demonstrate that electrophile reactivity correlates with the hydrophobicity of the substituted side chain. These mutations improve the selectivity for unnatural substrates as compared to KDPG by up to 2000‐fold. Furthermore, the S184L KDPG aldolase mutant improves the catalytic efficiency for the synthesis of a precursor for nikkomycin by 40‐fold, making it a useful biocatalyst for the preparation of fine chemicals.
The Effect of Molecular Environment on the Photoisomerization of Urocanic Acid ¶

Urocanic acid, imidazole propenoic acid, is a metabolic product of histidine, which accumulates in skin and is excreted in sweat. It absorbs UV radiation at wavelengths shorter than 340 nm, and its principal photochemical reaction is a trans‐cis isomerization about the propenyl double bond. This isomerization to the biologically active cis isomer is implicated in the photo‐induced suppression of the immune system of skin. The kinetics of the trans→cis photoisomerization of urocanic acid has been determined in a number of solvents, spanning a range of polarities. The initial rates of isomerization and the photostationary trans‐cis compositions, in all solvents except water, correlate linearly with solvent polarity. This indicates that the isomerization proceeds through a polar intermediate that is stabilized by coulombic interactions with the molecular environment.
Fistulae following laryngectomy in patients treated with irradiation

The development of fistulae following 197 consecutive laryngectomies performed from 1980 to 1987 have been examined. Fistulae were present in 28 patients (14 per cent). Age, T-classification, daily level of radiation dose and the time lapse between diagnosis and operation did not seem to influence the rate of fistula development. The study indicates that there is a two to three per cent risk of fistula development when a primary laryngectomy is performed. Fistula formation increased to about 10 to 12 per cent following radiotherapy, with an additional increase whenever previous operations on the neck had been done and/or if the disease demanded more extensive surgery. The study also indicates that the risk for fistula development is less when an experienced surgeon performs the operation.
A tale of two trajectories: bottom-up social software adoption in differing organisational contexts

The inherent bottom-up nature of social software diffusion differs markedly from the adoption trajectories to which most organisational IT strategies are accustomed. Through case studies in two organisations, this paper presents insights into how adoption of emergent social software platforms may take form, and discusses the implications of two radically different approaches to manage these processes. Our findings suggest that organisations may need to cede control over parts of the IT innovation adoption process to benefit from grassroots initiatives, and rather standardise successful innovations on an ongoing basis to avoid detrimental fragmentation of media usage across organisational boundaries.
Symptomatic zinc deficiency in breast‐fed term and premature infants

Two 3‐month‐old exclusively breast‐fed infants, one born at full‐term and the other born prematurely, developed symptomatic zinc deficiency manifested by an acrodermatitis enteropathica‐like eruption. Inadequate breast milk zinc was demonstrated in both cases. A rapid clinical response followed oral zinc supplementation after which their serum zinc levels returned to normal. The infants remained asymptomatic following cessation of zinc therapy. Reports of similar cases suggest that in a group of infants breast milk does not meet their nutritional zinc requirements. Inadequate breast milk zinc is thought to result from a defect in transfer of zinc from maternal serum to breast milk.
Picosecond dynamics of the glutamate receptor in response to agonist‐induced vibrational excitation

Conformational changes of proteins are dominated by the excitation and relaxation processes of their vibrational states. To elucidate the mechanism of receptor activation, the conformation dynamics of receptors must be analyzed in response to agonist‐induced vibrational excitation. In this study, we chose the bending vibrational mode of the guanidinium group of Arg485 of the glutamate receptor subunit GluR2 based on our previous studies, and we investigated picosecond dynamics of the glutamate receptor caused by the vibrational excitation of Arg485 via molecular dynamics simulations. The vibrational excitation energy in Arg485 in the ligand‐binding site initially flowed into Lys730, and then into the J‐helix at the subunit interface of the ligand‐binding domain. Consequently, the atomic displacement in the subunit interface around an intersubunit hydrogen bond was evoked in about 3 ps. This atomic displacement may perturb the subunit packing of the receptor, triggering receptor activation. Proteins 2004;54:000–000. © 2003 Wiley‐Liss, Inc.
Hypercalcemia in Malignancies: Treatment with Dichloromethylene Diphosphonate (Cl2MDP)

Eleven hypercalceic patients affected by various kinds of cancer were treated with dichloromethylene diphosphonate (Cl2MDP), 400 mg diluted in 500 ml of saline solution, i.v. , during 3 h. Drug administration normalized plasma calcium levels in all treated patients, within 72 h for patients with lung cancer; within a longer period for other kinds of cancer. Moreover, Cl2MDP also reduced calciuria in treated patients. The use of the drug was not followed by side effects and was also well tolerated in patients with kidney insufficiency.
Activation of Rac1 by phosphatidylinositol 3‐kinase in vivo: role in activation of mitogen‐activated protein kinase (MAPK) pathways and retinoic acid‐induced neuronal differentiation of SH‐SY5Y cells

Rho GTPases such as RhoA, Rac1 and Cdc42 are crucial players in the regulation of signal transduction pathways required for neuronal differentiation. Using an in vitro cell culture model of neuroblastoma SH‐SY5Y cells, we demonstrated previously that RhoA is an in vivo substrate of tissue transglutaminase (TGase) and retinoic acid (RA) promoted activation of RhoA by transamidation. Although activation of RhoA promoted cytoskeletal rearrangement in SH‐SY5Y cells, it was not involved in induction of neurite outgrowth. Here, we demonstrate that RA promotes activation of Rac1 in SH‐SY5Y cells in a transamidation‐independent manner. RA‐induced activation of Rac1 is mediated by phosphatidylinositol 3‐kinase (PI3K), probably because of phosphorylation of the p85 regulatory subunit by Src kinases. Over‐expression of constitutively active PI3K or Rac1‐V12 induces neurite outgrowth, activation of mitogen activated protein kinases (MAPKs), and expression of neuronal markers. The PI3K inhibitor LY294002, or over‐expression of dominant negative Rac1‐N17, blocks RA‐induced neurite outgrowth, activation of MAPKs, and expression of neuronal markers, suggesting that activation of PI3K/Rac1 signaling represents a potential mechanism for regulation of neuronal differentiation in SH‐SY5Y cells.