Effect of adding different levels of green tea infusion to semen extenders on some characteristics of the chilled-stored Awassi ram semen

The aim of this study was to assess the effect of adding different levels of green tea (GT) infusion to semen extenders on the percentage of motility, viability and abnormality of ram semen during storage at 5 ◦ C for 0, 24, 48, 72, 96, 120 and 144 hrs after collection and dilution. A total of 16 ejaculates from 3 Awassi rams were gathered by artificial vagina and extended by TRIS extender. The GT infusion was added by 0, 2, 4 and 6 ml/ 100 ml of extender which represents control group (C) and treatment groups (T1, T2 and T3) respectively. The results demonstrated a significant increase (P<0.05) in the percentage of sperm motility for each of the treatments T2 and T3 as compared with control group (C) for periods 0, 24, 48, 72, 96 and 120 hrs, while the treatment T1 showed a calculation increased for the same trait as compared with the control group (C) for all periods of experiment. The percentage of sperm viability showed a calculation increased for all treated groups T1, T2 and T3 as compared with control group (C) for all periods of experiment. Otherwise, the results appeared a significant decrease (P<0.05) in the sperm abnormalities percentage for both treatments T2 and T3 as compared with the control group (C) for all periods of experiment, whereas the treatment T1 revealed a significant decrease (P<0.05) for both periods 120 and 144 hrs as compared with control group (C) for the same trait. Therefore, it was concluded that adding GT infusion to the ram semen extenders can be used to enhance semen characteristics which include motility and viability as well as reduce the abnormality of ram semen stored at 5°C. ضعب ىلع يونولا لئاسلا تاففخه ىلا رضخلاا ياشلا عيقن نه ةفلتخه تايوتسه ةفاضا ريثات ةيساوعلا شابكلل ذيربتلاب ظوفحولا يونولا لئاسلا صئاصخ ةصلاخلا ىلع يىٌولا لئبسلا ثبففخه ىلا شضخلاا يبشلا عٍقً يه تفلتخه ثبٌىتسه تفبضا شٍثأت نٍٍقتل ىه تٍلبحلا تساسذلا يه فذهلا لا مبٌغلاا يهبٍح ثبهىشتو تٌىٍحو تكشحل تٌىئولا تبسٌلا ةساشح تجسد ىلع بهضفح ذٌع تٍساىع 5 م ◦ ،شفص ةذول 24 ، 48 ، 72 ، 96 ، 120 و 144 عوج نت . فٍفختلاو عوجلا ذعب تعبس 16 يه تفزق 3 نتو ًعبٌطصلاا لبهولا ماذختسبب تٍساىع شببك Husam J. H. Banana College of Agriculture/ University of Baghdad Hazim K. J. Al-Saab College of Agriculture/ University of Baghdad Firas A. Mahmood College of Agriculture/ University of Baghdad هنانب نيسح نساج ماسح تعاسضلا تٍلك – داذغب تعهبج بعصلا رساج راسك مزاح تعاسضلا تٍلك – داذغب تعهبج دووحه ذوحا سارف اسضلا تٍلك تع – داذغب تعهبج Euphrates Journal of Agriculture Science-7 (4): 1-9 , (2015) Banana et al. ____________________________________________________________________________________ 2 ISSN 2072-3875 ففخه ماذختسبب بهفٍفخت TRIS و شفص ساذقوب شضخلاا يبشلا عٍقً تفبضا نت . 2 و 4 و 6 /له 100 خولا يه له ًتلاو فف ةشطٍسلا تعىوجه لثوت (C) ثلاهبعولا يه لكو T1 و T2 و T3 . ًلاىتلا ىلع تٌىٌعه ةدبٌص دىجو ىلا جئبتٌلا ثسبشا (P<0.05) ثلاهبعولا يه لكل يهبٍحلا تكشحل تٌىئولا تبسٌلا ًف T2 و T3 تًسبقه ةشطٍسلا تعىوجه عه (C) ثاشتفلل 0 ، 24 ، 48 ، 72 ، 96 و 120 سبشا يٍح ًف ،تعبس ىلولاا تلهبعولا ث T1 ةدبٌص دىجو ىلا ةشطٍسلا تعىوجه عه تًسبقه تفصلا سفٌل تٍببسح (C) ةدبٌص يهبٍحلا تٌىٍحل تٌىئولا تبسٌلا ثشهظا ذقو . تبشجتلا ثاشتف لكل ثلاهبعولا عٍوجل تٍببسح T1 ، T2 و T3 ةشطٍسلا تعىوجه عه تًسبقه (C) ثشهظا ذقف ،كلر ىلع ةولاع . تبشجتلا ثاشتف لكل يىٌعه ضبفخًا دىجو جئبتٌلا (P<0.05) يٍتلهبعولا يه لكل يهبٍحلا ثبهىشتل تٌىئولا تبسٌلا ًف T2 و T3 عه تًسبقه ةشطٍسلا تعىوجه (C) يٍتشتفلا يه لكل يىٌعه ضبفخًا دىجو ىلا ىلولاا تلهبعولا ثسبشا يٍح ًف ،تبشجتلا ثاشتف عٍوجل 120 و 144 ةشطٍسلا تعىوجه عه تًسبقه تعبس (C) ثبففخه ىلا شضخلاا يبشلا عٍقً تفبضا ىبب جتٌتسً ازل . تفصلا سفٌل ىلا تفبضلابب تٌىٍحلاو تكشحلا لوشت ًتلاو يىٌولا لئبسلا صئبصخ يٍسحتل مذختسٌ ىا يكوٌ تٍساىعلا شببكلل يىٌولا لئبسلا ةساشح تجسد ىلع تظىفحولا شببكلا يهبٍح ثبهىشت لٍلقت 5 م ◦ .