Miracle-dz/UniFAR-FTUs huggingface dataset:
Alcohol and aviation.

Aviation accidents due to alcohol consumption by aircrew appear to be rare, especially in commercial aviation. However, a small proportion of general aviation accidents are attributable to alcohol use by aircrew, and aircrew are not well informed about the metabolism of alcohol and its effects on performance. Furthermore, there is evidence that aircrew performance may be impaired by alcohol consumption even after their Blood Alcohol Concentration has returned to "zero" (i.e. < 5 mg/dl). Accidents caused by impairment of aircrew performance by alcohol may therefore not be attributed to alcohol use at all. Aviation safety relies upon faultless human performance and is thus highly sensitive to alcohol-related impairment of performance. This paper provides a review of research regarding aircrew alcohol consumption, impairment of aircrew performance by alcohol, incidence of aviation accidents attributable to alcohol use by aircrew, and other related subjects.
Intra‐ and inter‐network functional alterations in Parkinson's disease with mild cognitive impairment

Mild cognitive impairment (MCI) is prevalent in 15%–40% of Parkinson's disease (PD) patients at diagnosis. In this investigation, we study brain intra‐ and inter‐network alterations in resting state functional magnetic resonance imaging (rs‐fMRI) in recently diagnosed PD patients and characterise them as either cognitive normal (PD‐NC) or with MCI (PD‐MCI). Patients were divided into two groups, PD‐NC (N = 62) and PD‐MCI (N = 37) and for comparison, healthy controls (HC, N = 30) were also included. Intra‐ and inter‐network connectivity were investigated from participants’ rs‐fMRIs in 26 resting state networks (RSNs). Intra‐network differences were found between both patient groups and HCs for networks associated with motor control (motor cortex), spatial attention and visual perception. When comparing both PD‐NC and PD‐MCI, intra‐network alterations were found in RSNs related to attention, executive function and motor control (cerebellum). The inter‐network analysis revealed a hyper‐synchronisation between the basal ganglia network and the motor cortex in PD‐NC compared with HCs. When both patient groups were compared, intra‐network alterations in RSNs related to attention, motor control, visual perception and executive function were found. We also detected disease‐driven negative synchronisations and synchronisation shifts from positive to negative and vice versa in both patient groups compared with HCs. The hyper‐synchronisation between basal ganglia and motor cortical RSNs in PD and its synchronisation shift from negative to positive compared with HCs, suggest a compensatory response to basal dysfunction and altered basal‐cortical motor control in the resting state brain of PD patients. Hum Brain Mapp 38:1702–1715, 2017. © 2016 Wiley Periodicals, Inc.
Acceptance and Continuation of Contraceptive Methods Immediate Postabortion

Background/Aims: Providing preabortion contraceptive counseling for the method of contraception may reduce the incidence of repeat abortions. This study aimed to compare the acceptance and continuation rates of intrauterine devices (IUDs), combined oral contraceptives (COCs), and condom use after abortion in women who received preabortion contraceptive counseling. Methods: Women seeking a first-trimester abortion prospectively received preabortion contraceptive counseling and the choice of method of contraception was based on the counseling and not related to prior methods of birth control. Outcomes included continuation of postabortion contraceptive use and repeat abortions over a 6-month follow-up period. Results: Four hundred and fifty-five women (IUD group: n = 161; COC group: n = 149; condom group: n = 135) completed 6-month follow-up after the abortions. At the sixth month follow-up, patients continued using the IUD, COC, and condoms for contraception were 64.2, 10, and 51.5%, respectively. Higher age, being married, parity ≥1, and previous abortion were factors that were associated with more frequent selection of an IUD, while a higher education level was associated with a more frequent selection of COC and condoms. Conclusion: The continued use of COCs after abortion is low even with preabortion contraceptive counseling. The IUD offers reliable birth control with a lower discontinuation rate than COCs or condoms.
Antiretroviral treatment regimens and immune parameters in the prevention of systemic AIDS-related non-Hodgkin's lymphoma.

PURPOSE Immunosuppression induced by HIV-1 increases the risk of developing non-Hodgkin's lymphoma (NHL). We measured the influence of immunologic factors and highly active antiretroviral therapy (HAART) on this risk. As there are no data demonstrating that specific antiretroviral regimens are effective at protecting from NHL, we compared different HAART regimens. PATIENTS AND METHODS The protective effect of HAART regimens, containing protease inhibitors (PI) and/or non-nucleoside reverse transcriptase inhibitors (NNRTIs) on the development of NHL was examined in a prospectively recorded cohort of 9,621 HIV-infected individuals. Lymphocyte and natural killer subset data were also entered in univariate and multivariate analyses to establish and stratify the risk of NHL. RESULTS From this cohort of 9,621 patients, 102 have been diagnosed with systemic AIDS-related NHL since 1996, when HAART became freely available here. By univariate analysis, increased age, higher nadir CD4 and CD8 T-cell counts, CD19 B-cell count, CD16/56 natural killer count and exposure to NNRTI or PI containing HAART conferred significant protection against NHL (P <.05). In a multivariate analysis, age, nadir CD4 and CD8 T-cell counts, and exposure to HAART were independent predictors of risk of NHL (P <.02). NNRTI-based HAART (adjusted rate ratio, 0.4; 95% CI, 0.3 to 0.5) was as protective as PI-based HAART, and these were significantly more protective than nucleoside analogues alone (rate ratio, 0.5; 95% CI, 0.4 to 0.7) or no antiretrovirals (P <.001). CONCLUSION Effective HAART-induced maintenance of CD4 and CD8 counts protects from systemic AIDS-related NHL.
Fault heterogeneity and earthquake scaling

There is an on‐going debate in the seismological community as to whether stress drop is independent of earthquake size and this has important implications for earthquake physics. Here we investigate this question in a simple 2D cellular automaton that includes heterogeneity. We find that when the range of heterogeneity is low, the scaling approaches that of constant stress drop. However, clear deviations from the constant stress drop model are observed when the range of heterogeneity is large. Further, fractal distributions of strength show more significant departures from constant scaling than do random ones. Additionally, sub‐sampling the data over limited magnitude ranges can give the appearance of constant stress drop even when the entire data set does not support this. Our results suggest that deviations from constant earthquake scaling are real and reflect the heterogeneity of natural fault zones, but may not provide much information about the physics of earthquakes.
Adherence to treatment guidelines in Type 2 diabetes patients failing metformin monotherapy in a real-world setting

SUMMARY Aim: To describe the drug management of T2DM patients in a real life cohort with suboptimal HbA1c after treatment with metformin monotherapy. Methods: we performed a retrospective cohort analysis of computerized medical records after measuring an HbA1c >7% for the first time following at least 90 days on metformin therapy. Results: Among 7705 eligible patients, 56% (n = 4336) changed treatment within 1-year, by increasing metformin dose (36%), adding drugs (60%), or switching to other medications (4%). Strongest predictors of change were higher HbA1c, younger age and higher socioeconomic status (SES). Conclusion: In this cohort, the extent of inertia appears to be smaller than that reported in previous studies. Nonetheless, disease management programs aimed at improving guideline adherence and reducing inertia are still warranted.
Overcoming Barriers to Arteriovenous Fistula Creation and Use

ABSTRACT National guidelines advocate the placement of arteriovenous fistulas (AVFs) as the preferred vascular access for hemodialysis (HD) patients because of their low complication rate, lower costs, and prolonged patency, once matured. The current Dialysis Outcomes Quality Initiative (DOQI) guidelines aim for an AVF incidence of 50% and a 40% prevalence in the United States. Although patients currently starting dialysis do so at an increasingly older age and with more comorbidity, they should be given every opportunity to receive an AVF. Meeting this challenge is facilitated by a multidisciplinary approach with early referral to the nephrologist in the predialysis period for access planning. Key components of a vascular access program may include the coordination by a dedicated access coordinator and outcome tracking via a prospective database. Preoperative vessel evaluation and careful selection of an appropriate surgical site, along with an experienced surgeon, improve surgical outcomes. Transposed brachiobasilic or other tertiary fistulas should be offered to patients who cannot receive a native radiocephalic or brachiocephalic fistula. The ability to routinely monitor and salvage failing AVFs is important to achieving successful AVF outcomes. Standardized definitions of AVF outcomes are important to allow individual centers and continuous quality assurance (CQA) programs to track and benchmark their outcomes against local and national standards to help them meet recommended targets.
Control of in vitro and in vivo spread of coxsackievirus B4 infection by sensitized spleen cells and antibody.

The effects of antibody and immune spleen cells of mice on the spread of coxsackievirus B4 in monkey kidney tissue cultures and mice were investigated. The spread of the virus in vitro was decreased both by antibody and by sensitized spleen cells, and the antiviral effect persisted even when the immune serum or immune cells were separated from the virus-infected monolayer by agar. The antiviral effect of the spleen cells was blocked by treatment of cells with antiserum to mouse IgG, but treatment of cells with antiserum to thymocytes had no effect. Non-glass-adherent cells were more capable of limiting the spread of coxsackievirus in cell cultures than were glass-adherent cells. Migration of sensitized splenic leukocytes in vitro was inhibited by specific antigen beginning three or four weeks after various doses of coxsackievirus B4. Adoptive transfer of antiserum protected 80%-100% of recipient mice, but spleen cells from only some of the mice protected 20%-66% of recipient mice against challenge with coxsackievirus B4. Thus, immunized mice develop sensitized T (thymus-derived) and B (bone marrow-derived) cells in the spleen. The B cells elaborate antibody to coxsackievirus, which can act locally to control the spread of infection. These results suggest that antibody plays a critical role in the control of the spread of coxsackievirus infection.
WHAT DOES MYSTICISM HAVE TO TEACH US ABOUT CONSCIOUSNESS

A key strategy for understanding a complex phenomenon is to look at its simplest manifestations. The gene structure of E. coli, for example, has contributed significantly to our understanding of gene functioning in more complex organisms. Mystical experiences may represent the simplest form of human consciousness and thus, by the same token, may provide valuable insights into the nature of human consciousness.
Associations of glycated haemoglobin A1c and glycated albumin with subclinical atherosclerosis in middle‐aged and elderly Chinese population with impaired glucose regulation

The aim of this study was to investigate the correlations of glycated haemoglobin A1c (HbA1c) and glycated albumin (GA) with subclinical atherosclerosis in middle‐aged and elderly Chinese populations with impaired glucose regulation (IGR). In total, 640 subjects with IGR and no history of cardiovascular disease or carotid artery plaque were recruited for this study (256 men, 384 women; age range, 40–70 years). The carotid intima‐media thickness (C‐IMT) measured by carotid ultrasonography was used as an indicator of subclinical atherosclerosis. Increased C‐IMT was defined as ≥ 0.70 mm (upper quartile). HbA1c and GA were measured with high‐performance liquid chromatography and enzymatic method, respectively. The average HbA1c and GA among all 640 subjects were 5.7 ± 0.3% and 14.0 ± 1.1%, respectively. HbA1c and GA were higher in subjects with increased C‐IMT than in subjects with normal C‐IMT (5.8 ± 0.3% vs 5.7 ± 0.3% and 14.2 ± 1.0% vs 13.9 ± 1.1%, respectively; both P < 0.01). Correlation analysis showed that both HbA1c and GA were positively correlated with C‐IMT (r = 0.135 and 0.112, respectively; both P < 0.01). Logistic regression analysis revealed that both HbA1c (odds ratio (OR), 2.630; 95% confidence interval (95% CI), 1.401–4.935; P = 0.003) and GA OR, 1.215; 95% CI, 1.008–1.466; P = 0.041) were independent risk factors for increased C‐IMT. Both HbA1c and GA reflect the risk of subclinical atherosclerosis in middle‐aged and elderly Chinese populations with IGR.
Anaphylaxis Management in the Pediatric Emergency Department: Opportunities for Improvement

Purpose: To determine the rate, immediate treatment, and outpatient management for anaphylaxis in patients receiving care in a pediatric emergency department (ED). Methods: This is a retrospective cross-sectional descriptive study of patients (21 years or younger) who received care for anaphylaxis for a 5-year period in the ED of the Children's Hospital of Alabama in Birmingham, AL, which has an annual census of 55,000. The diagnostic criteria for anaphylaxis were symptoms and/or signs involving 2 or more organ systems (dermatologic, respiratory, gastrointestinal, and cardiovascular), hypotension for age, 1 organ system involvement with admission to the hospital, and/or dermatologic system involvement treated with intramuscular epinephrine. Results: There were 124 patient visits by 103 patients (4.5 events/10,000 ED patient visits) who met the diagnostic criteria for anaphylaxis. This included 114 (92%) patients who had involvement of two or more organ systems. There were 66 (64%) males and 33 (27%) patient visits that resulted in hospitalization. The most common organ system involvement was dermatologic in 121 (98%), followed by respiratory in 101 (81%), gastrointestinal in 33 (27%), and cardiovascular in 11 (9%). Medical interventions include 69 patients treated with intramuscular epinephrine (56%; either in pre-hospital setting and/or during ED visit), 97 patients treated with corticosteroids (79%), 114 patients treated with H1 and/or H2 antihistamine (93%), 15 patients treated with intravenous fluid bolus (12%), and 37 patients treated with albuterol nebulization (30%). Food was the most common inciting allergen (in 45 or 36% of patients). Among the foods that were listed as causing reactions were peanuts, shellfish, milk, ice cream, fruit, nuts, and fried chicken. Compared with ED care-only patients, the hospitalized patients had a significantly greater rate of cardiovascular system involvement and of receiving more ED interventions. Of 91 ED care-only patients, autoinjection epinephrine was prescribed to 63% and referral to an allergist was recommended to 33%. Patients treated with intramuscular epinephrine had a significantly greater rate of hospitalization and of receiving more ED interventions compared with patients who were not treated with epinephrine. There were no patient deaths. Conclusions: This study is the first to describe the management of anaphylaxis in a pediatric ED. The results revealed opportunities for improvement. Although our ED treatment and outpatient management of patients with anaphylaxis did not meet the recommended standards of care with regard to administration of intramuscular epinephrine, prescribing autoinjection epinephrine, or referral to an allergist for all patients who had a diagnosis of anaphylaxis, we do report a higher concordance with published recommendations than those reported in previous studies performed in adults.
Ultrasound evaluation of carotid artery in primary hyperparathyroidism.

Patients with primary hyperparathyroidism (PHPT) have an increased cardiovascular morbidity and mortality. Elevated serum calcium and/or PTH may directly contribute to vascular tissue damage, but the role of classic factors for atherosclerosis has not fully been evaluated in this disease. The aim of our study was to dissect the potential effect of hypercalcemia and/or high PTH from that of major cardiovascular risk factors (i.e. diabetes mellitus, hyperlipidemia, hypertension, obesity, smoking habit) on the carotid artery structure of patients with PHPT. Twenty-six consecutive patients with PHPT [subdivided into two groups according to the absence (n = 10) or the presence (n = 16) of one or more risk factors] and 15 normocalcemic healthy subjects as controls were studied. At ultrasonography, a significant increase (P < 0.001) of carotid mean and maximum intima-media thickness, as well as a significant reduction of lumen diameter (P < 0.05) were found in the PHPT group with risk factors, compared with the other two groups. This suggests that hypercalcemia and/or PTH elevation per se are not determinant of carotid atherosclerosis in PHPT, and that increased cardiovascular mortality and morbility in this disease is attributable to the combined presence of classic cardiovascular risk factors.
A chick growth test for the evaluation of protein quality in cereal-based diets. 2. Comparison of the total protein efficiency method with the gross protein value method.

Synopsis Various protein concentrates have been evaluated by the Gross Protein Value (GPV) method and by the Total Protein Efficiency (TPE) method. The new method discriminated between the samples better than did the GPV, and correlations between the two tests were significant at the 1 per cent level for leaf protein concentrates and at the 5 per cent level for fish meals.
Evaluation of STAT5A Gene Expression in Aflatoxin B1 Treated Bovine Mammary Epithelial Cells.

PURPOSE Aflatoxin B1 (AFB1) is a potent mycotoxin which has been produced by fungi such as Aspergillus flavus and Aspergillus parasiticus as secondary metabolites due to their growth on food stuffs and induces hepatocellular carcinoma in many animal species, including humans. In the present study, the effect of AFB1 on STAT5A gene expression was investigated in bovine mammary epithelial cells using real time RT-PCR. METHODS Bovine mammary epithelial cells were seeded in a 24-well culture plate for three-dimensional (3D) culture in Matrigel matrix. After 21 days of 3D culture and reaching the required number of cells, cells were treated with AFB1 and incubated for 8 h. For real time PCR reaction, total RNA from the cultured and treated cells was extracted and used for complementary DNA synthesis. RESULTS The expression of STAT5A gene was significantly down regulated by AFB1 in dose- dependent manner and led to the reduction of proliferation and differentiation of epithelial cells, which has direct effect in milk protein quantity and quality. CONCLUSION According to the results, it seems that down regulation of STAT5A gene in AFB1-treated cells maybe due to DNA damage induced by AFB1 in bovine mammary epithelial cells.
Hyaluronic Acid Hydrogels for Articular Cartilage Defect Repair in a Large Animal Model

Introduction Intrinsic repair of articular cartilage damage is unsatisfactory. Due to limitations of current surgical treatments, tissue engineering (TE) approaches have been pursued as promising alternatives. Our laboratory investigates hyaluronic acid (HA) hydrogels as a scaffold for cartilage TE. When seeded with mesenchymal stem cells (MSCs) and cultured in defined media containing the chondrogenic factor transforming growth factor-b3 (TGF-b3) for several months, these constructs can reach near native biomechanical and biochemical properties. As opposed to pre-maturing these constructs prior to implantation, an alternative approach could be to encapsulate cells in HA hydrogels along with TGF-b3 laden microspheres (all polymerized in-situ) within a defect to induce differentiation and direct new matrix production in-vivo. Delivering such a high dose of TGF-b3 can induce cartilaginous matrix production in both in-vitro and subcutaneous invivo models. Our long-term goal is to compare these approaches directly within a clinically relevant in-vivo cartilage injury model. As a first step, the goal of this study was to assess the natural healing response to an HA hydrogels alone or delivering TGF-b3 in a large animal model of cartilage injury and repair.
A Multilaboratory, Multicountry Study To Determine Bedaquiline MIC Quality Control Ranges for Phenotypic Drug Susceptibility Testing

ABSTRACT The aim of this study was to establish standardized drug susceptibility testing (DST) methodologies and reference MIC quality control (QC) ranges for bedaquiline, a diarylquinoline antimycobacterial, used in the treatment of adults with multidrug-resistant tuberculosis. Two tier-2 QC reproducibility studies of bedaquiline DST were conducted in eight laboratories using Clinical Laboratory and Standards Institute (CLSI) guidelines. Agar dilution and broth microdilution methods were evaluated. Mycobacterium tuberculosis H37Rv was used as the QC reference strain. Bedaquiline MIC frequency, mode, and geometric mean were calculated. When resulting data occurred outside predefined CLSI criteria, the entire laboratory data set was excluded. For the agar dilution MIC, a 4-dilution QC range (0.015 to 0.12 μg/ml) centered around the geometric mean included 95.8% (7H10 agar dilution; 204/213 observations with one data set excluded) or 95.9% (7H11 agar dilution; 232/242) of bedaquiline MICs. For the 7H9 broth microdilution MIC, a 3-dilution QC range (0.015 to 0.06 μg/ml) centered around the mode included 98.1% (207/211, with one data set excluded) of bedaquiline MICs. Microbiological equivalence was demonstrated for bedaquiline MICs determined using 7H10 agar and 7H11 agar but not for bedaquiline MICs determined using 7H9 broth and 7H10 agar or 7H9 broth and 7H11 agar. Bedaquiline DST methodologies and MIC QC ranges against the H37Rv M. tuberculosis reference strain have been established: 0.015 to 0.12 μg/ml for the 7H10 and 7H11 agar dilution MICs and 0.015 to 0.06 μg/ml for the 7H9 broth microdilution MIC. These methodologies and QC ranges will be submitted to CLSI and EUCAST to inform future research and provide guidance for routine clinical bedaquiline DST in laboratories worldwide.
Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-Producing Escherichia coli Strains from Calves

ABSTRACT A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.