Miracle-dz/UniFAR-FTUs huggingface dataset:
Adaptation of the Hydrocarbonoclastic Bacterium Alcanivorax borkumensis SK2 to Alkanes and Toxic Organic Compounds: a Physiological and Transcriptomic Approach

The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However, investigations of growth behavior and physiology of these bacteria when cultivated with n-alkanes of different chain lengths (C6 to C30) as the substrates are still lacking. Growth rates increased with increasing alkane chain length up to a maximum between C12 and C19, with no evident difference between even- and odd-numbered chain lengths, before decreasing with chain lengths greater than C19. Surface hydrophobicity of alkane-grown cells, assessed by determination of the water contact angles, showed a similar pattern, with maximum values associated with growth rates on alkanes with chain lengths between C11 and C19 and significantly lower values for cells grown on pyruvate. A. borkumensis was found to incorporate and modify the fatty acid intermediates generated by the corresponding n-alkane degradation pathway. Cells grown on distinct n-alkanes proved that A. borkumensis is able to not only incorporate but also modify fatty acid intermediates derived from the alkane degradation pathway. Comparing cells grown on pyruvate with those cultivated on hexadecane in terms of their tolerance toward two groups of toxic organic compounds, chlorophenols and alkanols, representing intensely studied organic compounds, revealed similar tolerances toward chlorophenols, whereas the toxicities of different n-alkanols were significantly reduced when hexadecane was used as a carbon source. As one adaptive mechanism of A. borkumensis to these toxic organic solvents, the activity of cis-trans isomerization of unsaturated fatty acids was proven. These findings could be verified by a detailed transcriptomic comparison between cultures grown on hexadecane and pyruvate and including solvent stress caused by the addition of 1-octanol as the most toxic intermediate of n-alkane degradation.
Measuring the benefits of knowledge management at the Financial Services Authority: a case study

While many knowledge management case studies and theoretical texts list benefits to an organization of the implementation of a knowledge management strategy, the literature provides little evidence that systems have been devised to measure those benefits. This paper presents a case study that attempted to demonstrate the benefits of knowledge management by measuring its impact. This work was carried out in the in-house legal division of the UK’s Financial Services Authority over a period of 18 months. A measurement model was devised based on concepts adapted from the Balanced Scorecard. This was the knowledge management benefits scorecard. The scorecard took each objective of the division’s knowledge management strategy and listed knowledge management activities necessary to achieve each objective. Next, likely benefits to individuals and the division arising from each knowledge management activity were listed. Methods of collecting qualitative and quantitative measurements data were then devised. Results obtained showed that benefits arising from some knowledge management activities could be clearly demonstrated. However, activities that required individuals to change their behaviour or take time to learn new skills were more problematical. More time would be necessary to demonstrate the benefits of knowledge management conclusively.
Endovascular Treatment of Bilateral Intracranial Vertebral Artery Dissecting Aneurysms Presenting With Subarachnoid Hemorrhage

BACKGROUND: Optimal management of bilateral vertebral artery dissecting aneurysms (bi-VDAs) causing subarachnoid hemorrhage (SAH) remains unclear. OBJECTIVE: To investigate the treatment methods and outcomes of bi-VDA causing SAH. METHODS: Seven patients were treated endovascularly for bi-VDA causing SAH. Treatment methods and outcomes were evaluated retrospectively. RESULTS: Two patients were treated with 2 overlapping stents for both ruptured and unruptured VDAs, 2 with 2 overlapping stents and coiling for ruptured VDA and with conservative treatment for unruptured VDA, 1 with internal trapping (IT) for ruptured VDA and stent-assisted coiling for unruptured VDA, 1 with IT for ruptured VDA and 2 overlapping stents for unruptured VDA, and 1 with IT for ruptured VDA and a single stent for unruptured VDA. None had rebleeding during follow-up (range, 15-48 months). All patients had favorable outcomes (modified Rankin Scale score, 0-2). On follow-up angiography at 6 to 36 months, 9 treated and 2 untreated VDAs revealed stable or improved state, whereas 3 VDAs in 2 patients showed regrowth. Of the 3 recurring VDAs, 1 was initially treated with IT but recurred owing to retrograde flow to the ipsilateral posterior inferior cerebellar artery (PICA), the second was treated with single stent but enlarged, and the last was treated with 2 overlapping stents and coiling but recurred from the remnant sac harboring the PICA origin. All 3 recurred VDAs were retreated with coiling with or without stent insertion. CONCLUSION: Bilateral VDAs presenting with SAH were safely treated with endovascular methods. However, endovascular treatment may be limited for VDAs with PICA origin involvement.
Autoregulation of ZEB2 expression for zearalenone production in Fusarium graminearum

Several Fusarium species produce the polyketide mycotoxin zearalenone (ZEA), a causative agent of hyperestrogenic syndrome in animals that is often found in F. graminearum–infected cereals in temperate regions. The ZEA biosynthetic cluster genes PKS4, PKS13, ZEB1 and ZEB2 encode a reducing polyketide synthase, a non‐reducing polyketide synthase, an isoamyl alcohol oxidase and a transcription factor respectively. In this study, the production of two isoforms (ZEB2L and ZEB2S) from the ZEB2 gene in F. graminearum via an alternative promoter was characterized. ZEB2L contains a basic leucine zipper (bZIP) DNA‐binding domain at the N‐terminus, whereas ZEB2S is an N‐terminally truncated form of ZEB2L that lacks the bZIP domain. Interestingly, ZEA triggers the induction of both ZEB2L and ZEB2S transcription. ZEB2L and ZEB2S interact with each other to form a heterodimer that regulates ZEA production by reducing the binding affinity of ZEB2L for the ZEB2L gene promoter. Our study provides insight into the autoregulation of ZEB2 expression by alternative promoter usage and a feedback loop during ZEA production; this regulatory mechanism is similar to that observed in higher eukaryotes.
Axonal projections of single auditory neurons in the thalamic reticular nucleus: implications for tonotopy‐related gating function and cross‐modal modulation

Tonotopically comparable subfields of the primary auditory area (AI) and nonprimary auditory areas (non‐AI), i.e. posterodorsal area (PD) and ventral auditory area (VA), in the rat cortex have similar topographies in the projection to the ventral division of the medial geniculate nucleus (MGV), but reverse topographies in the projection to the thalamic reticular nucleus (TRN). In this study, we examined axonal projections of single auditory TRN cells, using juxtacellular recording and labeling techniques, to determine features of TRN projections and estimate how the TRN mediates corticofugal inhibition along with the reverse topographies of cortical projections to the TRN. Auditory TRN cells sent topographic projections to limited parts of the MGV in a manner that relays cortical inputs from tonotopically comparable subfields of the AI and non‐AI (PD and VA) to different parts of the MGV. The results suggest that corticofugal excitations from the AI and non‐AI modulate thalamic cell activity in the same part of the MGV, whereas corticofugal inhibitions via the TRN modulate cell activity in different parts of the MGV with regard to tonotopic organization. The AI and non‐AI could serve distinctive gating functions for auditory attention through the differential topography of inhibitory modulation. In addition, we obtained an intriguing finding that a subset of auditory TRN cells projected to the somatosensory but not to the auditory thalamic nuclei. There was also a cell projecting to the MGV and somatosensory nuclei. These findings extend the previously suggested possibility that TRN has a cross‐modal as well as an intramodal gating function in the thalamus.
Dynamism, Capital Structure, and Performance in a Sub-Saharan Economy: Extending the Institutional Difference Hypothesis

Extant research examining the capital structure–performance relationship has been undertaken primarily in developed economies. These studies integrate agency theory considerations with the contingency perspective and suggest a positive interaction between sector dynamism, equity, and performance. Our study extends this research stream by examining the capital structure–performance link in a sub-Saharan economy, an underresearched but economically emerging region. Using logic undergirding a substantial stream of institutional theory-based studies that we term the institutional difference hypothesis , we argue that the implications of the capital structure–performance relationship are contingent on the extent of national-level institutional underdevelopment. Taking into account institutional differences between developed economies and those in the sub-Saharan region, we hypothesize a negative interaction between sector dynamism, equity, and firm performance in sub-Sahara. Using longitudinal data from Ghanaian corporations for 1996–1999, we find substantial support for our hypothesis. Contrary to findings from developed economies, sector dynamism negatively moderates the firm equity–performance relationship: a strongly positive effect of equity on performance in stable sectors becomes slightly negative in highly dynamic ones. We unpack the implications of these results for theory and research.
Vitamin D status among indigenous Mayan (Kekchi) and Afro-Caribe (Garifuna) adolescents from Guatemala: a comparative description between two ethnic groups residing on the Rio Dulce at the Caribbean coast in Izabal Province, Guatemala

Abstract Objective To assess vitamin D status and the influence of risk factors such as skin pigmentation and time spent outdoors on hypovitaminosis D among Guatemalan Kekchi and Garifuna adolescents. Design Cross-sectional study, with convenient sampling design. Blood samples, anthropometric and behavioural data were all collected during the dry season. Serum 25-hydroxyvitamin D (25(OH)D) concentrations were measured by RIA. Setting Communities of Rio Dulce and Livingston, Izabal Province, Caribbean coast of Guatemala, with latitude and longitude of 15°49′N and 88°45′W for Livingston and 15°46′N and 88°49′W for Rio Dulce, respectively. Subjects Eighty-six adolescents, divided evenly by sex and ethnicity, with mean age of 14 years. Results Mean (sd) 25(OH)D value was 27·8 (7·2) ng/ml for the total group, with 25·8 (5·9) and 29·8 (7·9) ng/ml, respectively, in Kekchis and Garifunas (P=0·01). Use of vitamin D supplementation, clothing practices and sun protection were not statistically different between groups. Skin area exposed on the day of data collection ranged from 20·0 % minimum to 49·4 % maximum, with mean (sd) exposure of 32·0 (8.5) %. With univariate regression analysis, age (P=0·034), sex (P=0·044), ethnicity (P=0·010), time spent outdoors (P=0·006) and percentage skin area exposed (P=0·001) were predictive. However, multivariate analysis indicated that only sex (P=0·034) and percentage skin area exposed (P=0·044) remained as predictors of 25(OH)D. Conclusions Despite residing in an optimal geographic location for sunlight exposure, nearly 65 % of study adolescents were either insufficient or deficient in vitamin D. Correction and long-term prevention of this nutritional problem may be instrumental in avoiding adverse effects in adulthood attributed to low 25(OH)D during adolescence.
Short-Term Pulmonary Response to Inhaled JP-8 Jet Fuel Aerosol in Mice

B6.A.D. (Ahrd/Nat s ) mice were utilized to investigate the short-term pulmonary response to JP-8 jet fuel (JP-8) aerosol inhalation. Mice were nose-only exposed to atmospheres of 0 to 118 mg/m3 for 1 h/d over a period of 7 days to further test the hypothesis that JP-8 concentrations below the permissible exposure level (PEL) of 350 mg/m3 will induce lung injury. At 24 to 30 hours after the final exposure, pulmonary function and respiratory permeability were measured on anesthetized mice and then randomly assigned for bronchoalveolar lavage or histopathology. Bronchoalveolar lavage fluid (BALF) was analyzed for total protein, lactic dehydrogenase (LDH), N-acetyl-β-D-glucosaminidase (NAG), and cytology. Respiratory permeability increases were observed following doses of 48 and 118 mg/m3 and were supported by concomitant BALF increases in total protein and LDH. Conversely, NAG and alveolar macrophage levels decreased following the same exposure concentrations. Morphological lung injury was characterized by the targeting of bronchiolar epithelium and consisted of perivascular edema, Clara cell vacuolization, and necrosis. Alveolar injury included sporadic pulmonary edema, intra-alveolar hemorrhage, and alterations in type II epithelial cells. These results indicate that repeated inhalation of aerosolized JP-8 induces physiological, biochemical, cellular, and morphological lung injury. This study also provides evidence for the reevaluation of the 350 mg/m3 PEL for more volatile petroleum distillates with regard to respirable aerosols.
Malignant mesothelioma in North America

Ascertainment, through 7,400 pathologists, of all fatal malignant mesothelial tumors in Canada (1960–75) and the U.S.A. (1972) gave a total of 668 cases (272 in 1972). In Canada, the annual number of male cases rose from about 17 in 1966 to 25 in 1972 but the number of female cases remained fairly steady at a much lower level. The annual incidence in North America in 972 was estimated at 2.8 per million males and 0.7 per million females aged 15 years and over. Occupational histories were obtained “blind” for 480 of the 557 cases through 1972, and their matched controls; relative risks were as follows: insulation work, 46.0, asbestos production and manufacture, 6.1, heating trades (other than insulation) 4.4. For nearly half the male cases and for about 5% of female cases, the tumor could be attributed to occupational exposure to asbestos, of which a fifth were in shipyards. No indication was found of other possible causes (including man‐made mineral fibers, tobacco smoking, or residence near zeolite deposits). Four subjects were men who had been employed in Quebec chrysotile mines and 3 were children of employees, but no other subject had lived in the mining area. The findings remain consistent with a much greater mesothelioma‐producing potential for crocidolite and amosite than for chrysotile; however, further studies of factory workers exposed to chrysotile only are needed to confirm this. Mineral fiber analysis of lung tissue from patients and controls is in progress.
Can Betweenness Centrality Explain Traffic Flow

Traffic flow is the process of physical agents moving along an urban travel network. These agents are autonomous, purposeful, flexible, and volatile. They establish a social network: agents near to each other can communicate and interact (other social ties, like kinship or friendship, are not considered here). Since the agents are mobile this social network is highly dynamic. Also agents are volatile. They enter traffic at any time, and leave as soon as they have reached their destination. The places where they emerge or disappear are distributed over space and time, but not in a random manner. Additionally, agents in urban traffic are purposeful. They have individual travel, sensing and communication capabilities, maybe even preferences, and a specific travel demand (to reach a destination by a specified time or specified costs). Especially, during travel they can interact with their fellow agents, be it by coordination (communication) or collaboration (transport), and they can sense and act in their physical environment, and thus, change their travel plans at any time to satisfy their travel demand. This means travel plans—if not the underlying travel demand itself—can be dynamic. This social network of agents in traffic can also be characterized by centrality measures; however, these measures are attributes of the agents, not of the nodes of the physical travel network, and they are constantly changing—hence, infeasible to track in a central database. With all these behavioral observations of urban traffic at hand, we are interested in whether, and if, to what extent the physical structure of the network, characterized by betweenness centrality, determines the traffic generated by the agents. Betweenness centrality is based on shortest paths in the network, and the typical (implicit) assumption of rational agents suggests expecting that they travel shortest paths. Accordingly one can expect that nodes on many shortest paths in the urban travel network attract much traffic, and by this way, betweenness centrality can characterize the patterns of traffic flow or traffic density. This view is supported by evidence reported in the literature (see, e.g., Hillier et al. 1993; Penn et al. 1998; Jiang 2008b). But two factors are shaking our confidence in this argument. First, we know that human agents act at most bounded rational (Gigerenzer 2008), and hence, their chosen distance function in determining shortest paths is likely not to be topological (alone). Second, the depicted dynamics of travel behavior cannot be found out of the characteristics of a static network. Therefore, whether betweenness centrality can explain traffic flow is a valid question. We will try to prove the hypothesis that betweenness centrality of the physical travel network is insufficient to explain traffic flow.
Reconfigurable topology synthesis for application-specific noc on partially dynamically reconfigurable FPGAs

In this paper, we propose a three-stage reconfigurable topology synthesis approach for Application-Specific NoC (ASNoC) on partially dynamically reconfigurable FPGAs, where the topology is reconfigured dynamically at run-time along with the application’s execution. Firstly, given the scheduling and floorplanning of task modules, an Integral Linear Programming (ILP)-based method is proposed to partition the communication requirements of the application into T time intervals. Secondly, we explore the proper positions of switches in the floorplan for global communications. Finally, considering the reconfiguration costs between adjacent time intervals, the routing path allocation problem is solved for time intervals in an iterative procedure to generate fine-grained dynamically reconfigurable ASNoC topologies for the given application. Experimental results show that, compared to the random partition of communication requirements, the proposed ILP-based method can achieve 12.1% power consumption improvement. And, the reconfigurable ASNoC can achieve 36.3% power consumption improvement when compared with static ASNoCs.
Genetic variation in immune function and susceptibility to human filariasis

The generation of a draft sequence of a the human genome has provided the opportunity to characterize human diversity, even as it pertains to differences in host response to parasitic infection with organisms that cause lymphatic filariasis, malaria and schistosomiasis. Worldwide, human infection with filarial pathogens represents a significant cause of morbidity throughout the tropics. In particular, epidemiologic evidence suggests that a genetic component contributes to susceptibility and possibly the outcomes of filarial infection. Different approaches can be applied in population-based studies in areas where filarial infection is endemic, such as genome linkage scans and candidate gene analysis for the purpose of identifying genetic risk factors. This review summarizes recent advances in our understanding of genetic contributions to human lymphatic filariasis and addresses the immediate questions facing the field. It is anticipated that the identification of susceptibility genes in filarial infection could provide new insights into therapeutic strategies, including pharmacological intervention and vaccine development, and influence public health measures to control or avert infection.
Noncanonical Effects of IRF9 in Intestinal Inflammation: More than Type I and Type III Interferons

ABSTRACT The interferon (IFN)-stimulated gene factor 3 (ISGF3) transcription factor with its Stat1, Stat2, and interferon regulatory factor 9 (IRF9) subunits is employed for transcriptional responses downstream of receptors for type I interferons (IFN-I) that include IFN-α and IFN-β and type III interferons (IFN-III), also called IFN-λ. Here, we show in a murine model of dextran sodium sulfate (DSS)-induced colitis that IRF9 deficiency protects animals, whereas the combined loss of IFN-I and IFN-III receptors worsens their condition. We explain the different phenotypes by demonstrating a function of IRF9 in a noncanonical transcriptional complex with Stat1, apart from IFN-I and IFN-III signaling. Together, Stat1 and IRF9 produce a proinflammatory activity that overrides the benefits of the IFN-III response on intestinal epithelial cells. Our results further suggest that the CXCL10 chemokine gene is an important mediator of this proinflammatory activity. We thus establish IFN-λ as a potentially anticolitogenic cytokine and propose an important role for IRF9 as a component of noncanonical Stat complexes in the development of colitis.
CORPORATE GOVERNANCE , VALUATION AND OWNERSHIP IN CHINA : AN EMPIRICAL STUDY OF LISTED FIRMS

The Chinese economy has undergone substantial and fundamental transformation as reforms are effected and the state monopoly is exchanged for more market oriented ownership structures. This research paper contributes to the ongoing body of work relating to corporate governance in China. The study investigates whether ownership changes, concentration or structure have significant influence on the performance of listed firms in China. The findings suggest that the market considers changes in ownership favourably, especially if the changes reduce state holdings. The market views the subsequent reduction in the influence of the state on enterprises in China positively. This then implies that legal person, tradable A or foreign holdings are considered superior to state ownership. It follows then that the structure of shareholdings is of importance and empirical evidence supports the notion. It is apparent that the function of legal persons is more highly regarded in the process of corporate governance in the market in China, as legal person blockholdings are found to be positively correlated with firm performance. Nevertheless, the study finds that ownership concentration per se, does not have explanatory power in relation to firm performance. Size is also found to be negatively correlated with performance, suggesting that the market identifies the unwieldy size of many of the large listed SOEs to be a hindrance to performance. Corporate Governance, Valuation and Ownership in China 2