Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression, purification and DNA-binding activities of two putative ModE proteins of Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)

In prokaryotes molybdenum is taken up by a high-affinity ABC-type transporter system encoded by the modABC genes. The endophyte β-Proteobacterium Herbaspirillum seropedicae has two modABC gene clusters and two genes encoding putative Mo-dependent regulator proteins (ModE1 and ModE2). Analysis of the amino acid sequence of the ModE1 protein of H. seropedicae revealed the presence of an N-terminal domain containing a DNA-binding helix-turn-helix motif (HTH) and a C-terminal domain with a molybdate-binding motif. The second putative regulator protein, ModE2, contains only the helix-turn-helix motif, similar to that observed in some sequenced genomes. We cloned the modE1 (810 bp) and modE2 (372 bp) genes and expressed them in Escherichia coli as His-tagged fusion proteins, which we subsequently purified. The over-expressed recombinant His-ModE1 was insoluble and was purified after solubilization with urea and then on-column refolded during affinity chromatography. The His-ModE2 was expressed as a soluble protein and purified by affinity chromatography. These purified proteins were analyzed by DNA band-shift assays using the modA2 promoter region as probe. Our results indicate that His-ModE1 and His-ModE2 are able to bind to the modA2 promoter region, suggesting that both proteins may play a role in the regulation of molybdenum uptake and metabolism in H. seropedicae.
Scheduling outpatients in hospital examination departments

Outpatients scheduling problem in examination departments allocates hospital equipments to patients and decides on the time to perform examination. This task plays an important role in utilizing the limited hospital equipments efficiently while ensuring quality of service for patients. First, the history examinations data of the hospital is analyzed, and the bottleneck department is defined. Then, some dispatching rules for the outpatient examination are developed to minimize total waiting time subject to minimum idle-time of the equipments in bottleneck department. Computational experiments are performed on the real data collected from Shanghai Ninth People Hospital, and the results show that it is efficient to reduce the waiting time of the outpatients.
Use of the Hand Test in the classification of psychiatric inpatient adolescents.

This study investigates the extent to which 2 Hand Test variables, the Pathology summary score (PATH) and the Acting Out Score (AOS), were able to differentiate among 3 groups of adolescents. Thirty psychiatric inpatient adolescents diagnosed with various Diagnostic and Statistical Manual of Mental Disorders (4th ed.; American Psychiatric Association, 1994) behavioral and emotional disorders were compared to 30 outpatient and 30 nonclinical adolescents matched on age, grade, and sex. An analysis of variance demonstrated the ability of both PATH and AOS to significantly differentiate among the 3 groups. Multiple regression equations utilized PATH and AOS as significant predictors of inpatient status (p < .0001) and general patient (inpatient and outpatient) status (p < .0001). Utilizing diagnostic efficiency statistics, overall correct classification was found to be highest for PATH > or = 3 and AOS > or = 2. These findings support the application of the Hand Test as a useful assessment tool for adolescents suspected of having psychiatric disorders and present evidence for the use of PATH and AOS for classification purposes in ways that are clinically meaningful.
Psychiatric morbidity among young offenders in England and Wales

third of the respondents had been working prior to coming to prison. The remainder mostly said they had been unemployed and looking for work or living off crime. More than eight out of 10 of the young offenders in the sample said that they currently smoked cigarettes. Just over one in 10 respondents said they had never drunk alcohol in the year before coming to prison, while over a quarter said they drank alcohol four or more times a week during that time. More than six out of 10 said they had used some illicit drug in the month before coming to prison. Very few of the young offenders had been in prison for two or more years during their current prison term (less than 2%) and the majority had been in prison for less than a year. Over half the young offenders were being held for acquisitive offences— burglary, robbery, and theft—while about one in five were being held for a violent offence. Among the women drug offences were also common: about one in five of the sample were being held for these offences.
Promotion Orientation Explains Why Future-Oriented People Exercise and Eat Healthy

The authors extended research linking individual differences in consideration of future consequences (CFC) with health behaviors by (a) testing whether individual differences in regulatory focus would mediate that link and (b) highlighting the value of a revised, two-factor CFC-14 scale with subscales assessing concern with future consequences (CFC-Future) and concern with immediate consequences (CFC-Immediate) proper. Exploratory and confirmatory factor analyses of the revised CFC-14 scale supported the presence of two highly reliable factors (CFC-Future and CFC-Immediate; αs from .80 to .84). Moreover, structural equation modeling showed that those high in CFC-Future engage in exercise and healthy eating because they adopt a promotion orientation. Future use of the two-factor CFC-14 scale is encouraged to shed additional light on how concern with future and concern with immediate consequences (proper) differentially impact the way people resolve a host of intertemporal dilemmas (e.g., health, financial, and environmental behavior).
THE CONNECTION BETWEEN THE HOST HALO AND THE SATELLITE GALAXIES OF THE MILKY WAY

Many properties of the Milky Way’s (MW) dark matter halo, including its mass-assembly history, concentration, and subhalo population, remain poorly constrained. We explore the connection between these properties of the MW and its satellite galaxy population, especially the implication of the presence of the Magellanic Clouds for the properties of the MW halo. Using a suite of high-resolution N-body simulations of MW-mass halos with a fixed final , we find that the presence of Magellanic Cloud-like satellites strongly correlates with the assembly history, concentration, and subhalo population of the host halo, such that MW-mass systems with Magellanic Clouds have lower concentration, more rapid recent accretion, and more massive subhalos than typical halos of the same mass. Using a flexible semi-analytic galaxy formation model that is tuned to reproduce the stellar mass function of the classical dwarf galaxies of the MW with Markov-Chain Monte-Carlo, we show that adopting host halos with different mass-assembly histories and concentrations can lead to different best-fit models for galaxy-formation physics, especially for the strength of feedback. These biases arise because the presence of the Magellanic Clouds boosts the overall population of high-mass subhalos, thus requiring a different stellar-mass-to-halo-mass ratio to match the data. These biases also lead to significant differences in the mass–metallicity relation, the kinematics of low-mass satellites, the number counts of small satellites associated with the Magellanic Clouds, and the stellar mass of MW itself. Observations of these galaxy properties can thus provide useful constraints on the properties of the MW halo.
“Hit-and-Run” Effects of Paramyxoviruses as a Basis for Chronic Respiratory Disease

Background: The traditional scheme for asthma pathogenesis depends on increased T helper type 2 (Th2) over T helper type 1 (Th1) responses to allergic and nonallergic stimuli and consequent airway inflammation, hyperreactivity and hypersecretion. Here we question whether the innate immune system, including airway epithelial cells, and the adaptive one may manifest an aberrant antiviral response as an additional basis for chronic inflammatory diseases, including asthma. Methods: We focused on the signal transduction and genetic basis for mucosal immunity, inflammation and remodeling, especially in relation to airway diseases. We concentrated on the response to paramyxoviruses because these agents are closely associated with common acute and chronic airway diseases. We used viral, cellular and mouse models, as well as human subjects, for study and made comparisons among these systems. Our approach aims to answer 2 major questions: (1) what are the factors that control acute paramyxoviral infection; and (2) how can these transient infections cause long term airway disease? Conclusions: Our studies show that antiviral defense depends on a special network of epithelial immune response genes that signal to the adaptive immune system. Viruses ordinarily trigger this network, but it is also permanently activated in asthma, even in the absence of viral infection. In addition, we find that, in susceptible genetic backgrounds, respiratory viruses cause a “hit-and-run” phenomenon indicated by the development of an asthmatic phenotype long after the infection has cleared. On the basis of this information, we developed a new scheme for asthma pathogenesis that includes epithelial, viral and allergic components and allows viral reprogramming of host behavior.
Relationship Between Neutrophil/Lymphocyte Ratio and Albuminuria in Diabetic Patients

MAterIAl and MetHODS: This study included 170 diabetic patients. The patients were divided 3 groups according to urinary albumin creatinine ratio (UACR). The first group included patients with normoalbuminuria (UACR <0.030). The second group included patients with moderately increased albuminuria (0.030-0.300), whereas the third group consisted of patients with severely increased albuminuria (UACR>0.300).
Diet and nutrition in polycystic ovary syndrome (PCOS): Pointers for nutritional management

Summary PCOS patients are not always markedly overweight but PCOS is strongly associated with abdominal obesity and insulin resistance. Effective approaches to nutrition and exercise improve endocrine features, reproductive function and cardiometabolic risk profile – even without marked weight loss. Recent studies allow us to make recommendations on macronutrient intake. Fat should be restricted to ≤30% of total calories with a low proportion of saturated fat. High intake of low GI carbohydrate contributes to dyslipidaemia and weight gain and also stimulates hunger and carbohydrate craving. Diet and exercise need to be tailored to the individual's needs and preferences. Calorie intake should be distributed between several meals per day with low intake from snacks and drinks. Use of drugs to either improve insulin sensitivity or to promote weight loss are justified as a short-term measure, and are most likely to be beneficial when used early in combination with diet and exercise.
Integrating Personality and Psychosocial Theoretical Approaches to Understanding Safer Sexual Behavior: Implications for Message Design

The purpose of this study was to propose and conduct tests of a multivariate model of condom use utilizing data from 2 independent samples of young adults (City 1, N = 746; City 2, N = 743). The model examined the relations between personality characteristics, including sexual sensation seeking and sexual impulsive decision making; psychosocial variables, including condom attitudes, norms, and self-efficacy; and condom use behaviors, including carrying, communicating about, and using condoms. Structural equation modeling analyses indicated a good fit for both models (Confirmatory Fit Index =. 93; Average Absolute Standardized Residuals =. 05 for both), with each explaining 25% of the variance (R 2 =. 25) in condom use behaviors. Results support the fusion of personality and psychosocial approaches to gain a broader theoretical understanding of condom use in young adults. In addition, those developing and implementing health communication campaigns may find sexual sensation seeking and sexual impulsive decision making to be fruitful variables on which to target messages aimed at increasing safer sexual behavior.
Role of educational technologies in reemergence of India as a knowledge society

India thrived as a knowledge society in the past. However, the colonial interlude extending over centuries systematically eroded her knowledge base. Though recently there has been a growing interest and awakening among the people and the government towards the goal to reemerge as a knowledge society, systematic solutions have been eluding us due mainly to our rigid structures of education, particularly the higher education. In this paper we discuss how knowledge centers, which can be developed within and outside our present structures of education, can harness education technologies, in synergy with the information and communication technologies, to facilitate the institutionalization of knowledge reforms in India and accelerate her march towards this ambitious goal. Recently we have taken a modest experimental initiative along this direction by starting a physics knowledge center in our institute. We also present here the encouraging results of this experiment.
Evaluation of the Selected 12-locus MIRU for Genotyping Beijing Family Mycobacterium tuberculosis in Korea

Background: Mycobacterial Interspersed Repetitive Units (MIRUs) that are located mainly in intergenic regions dispersed throughout the Mycobacterium tuberculosis genome. The selected MIRU loci, which were composed of a 12-locus set, demonstrated a high power for discrimination of Mycobacterium tuberculosis isolates collected from Kangwon province of Korea. To evaluate its ability to discriminate the M. tuberculosis strains, 45 clinical isolates were genotyped using the methods IS6110 RFLP and MIRU. Methods: All the samples were collected during the period from January 2007 to December 2007 from TB patients, who were residents and registered to a public health center of Kangwon Province in Korea. A total of 45 DNAs were extracted from clinical isolated mycobacterial strains and genotyped using IS6110 RFLP, the MIRU method. Results: We compared the 12-MIRU with IS6110 RFLP in the 45 samples, the 12-locus version offered less discriminatory power (Hunter-Gaston discriminatory index [HGDI]: 0.959 vs 0.998; 57.78% of clustered cases vs 8.89%). Conclusion: This 12-locus MIRU can be useful when additional combinations of other loci for genotyping M. tuberculosis in Korea where the Beijing family strains are dominant.
Normal cortisol response on low-dose synacthen (1 microg) test in children with Prader Willi syndrome.

INTRODUCTION It has been postulated that central adrenal insufficiency (CAI), resulting from hypothalamic dysfunction, may contribute to the increased unexplained death rates in Prader Willi syndrome (PWS). A study using the overnight metyrapone test reported a 60% prevalence of CAI in children with PWS. We used a low-dose Synacthen test to screen for CAI in children with PWS. METHODS We studied 41 children with genetic diagnosis of PWS [20 males; mean age, 7.68 (±5.23) yr] in five pediatric endocrinology centers in Australasia. All participants were randomly selected, and none had a history of Addisonian crisis. Ten of the cohort were receiving sex hormone therapy, 19 were receiving GH, and four were receiving T4. Their mean body mass index z-score was +1.48 (±1.68). Baseline morning ACTH and cortisol levels were measured, followed by iv administration of 1 μg Synacthen. Post-Synacthen cortisol levels were measured at 30 min, and a cortisol level above 500 nmol/liter was considered normal. RESULTS The mean baseline ACTH and cortisol were 15 (±14) ng/liter and 223 (±116) nmol/liter, respectively. The mean 30-min plasma cortisol was 690 (±114) nmol/liter, and the average increase from baseline was 201%. CONCLUSIONS Our result suggests that CAI is rare in children with PWS.
Sex Differences and the Heritability of Scute Pattern Abnormalities in the Green Sea Turtle from the Ogasawara Archipelago, Japan

Although scute pattern abnormalities in sea turtle species are considered to be strongly correlated with survival rate, there is little information available regarding these abnormalities and the primary cause for their development is unclear. For the conservation of sea turtle species, accumulating basic knowledge of scute pattern abnormalities is a fundamental step towards a better understanding of the causes of these abnormalities. In the present study, we counted vertebral and costal scutes from adults hunted for food (male and female) (n = 899), nesting females (n = 155), and hatchlings (n = 44,537) of the green sea turtle (Chelonia mydas) from the Ogasawara Archipelago, Japan. We found that the frequency of turtles with non-modal scute patterns was significantly greater in adult females than that in adult males (P = 0.02). Since females are produced by warmer incubation temperatures, and the period of sex determination coincides with the period of scute pattern determination, high incubation temperatures may be responsible for the induction of scute pattern abnormalities. Moreover, females with non-modal scute patterns produced a higher frequency of hatchlings with non-modal scute patterns than females with modal scute patterns (P < 0.01), indicating that scute abnormality may be heritable. For conservation of this species, our results suggest that decrease of the incubation temperature by cooling methods, such as provision of shade, may minimize the frequency of non-modal scute patterns, with consideration for the natural sex ratio.
Concentrations of some metabolites in the breath of healthy children aged 7–18 years measured using selected ion flow tube mass spectrometry (SIFT-MS)

Using selected ion flow tube mass spectrometry (SIFT-MS), measurements have been made of the levels of several metabolites in the exhaled breath of 200 healthy school children. Thus, concentration distributions of each metabolite have been obtained for the first time in the paediatric age range. The median values (in parentheses) of the concentrations in parts per billion, ppb, were ammonia (628), acetone (297), methanol (193), ethanol (187), isoprene (37), propanol (16), acetaldehyde (23) and pentanol (15). Hydrogen cyanide was not present in the breath above the detection limit of 2 ppb in the majority of subjects. The water vapour level (humidity) of the breath samples was routinely measured as a check on the sample integrity. Such data are essential if SIFT-MS breath analyses are to be used as a clinical tool to aid diagnosis and/or as a monitor of disease in children. The levels of metabolites usually followed a log-normal distribution and the levels of some compounds were similar to those obtained previously in adults. Lower values were found in the levels of acetone, ammonia, methanol and isoprene. There were no major variations in relation to gender. Some metabolites showed significant variation in relation to age and body mass index. To our knowledge, these are the first measurements of exhaled mouth breath pentanol levels. The median ammonia levels in mouth-exhaled breath of these children decreased with age, whereas in older adults, ammonia has been shown to increase with age. Breath acetone levels were significantly increased for those who had not eaten for more than 6 h prior to providing the breath sample, although dietary control was not a mandatory aspect of the protocol.
Differential effects of trivalent and pentavalent arsenicals on cell proliferation and cytokine secretion in normal human epidermal keratinocytes.

There is strong evidence from epidemiologic studies of an association between chronic exposure to inorganic arsenic (iAs) and hyperpigmentation, hyperkeratosis, and neoplasia in the skin. Although it is generally accepted that methylation is a mechanism of arsenic detoxification, recent studies have suggested that methylated arsenicals also have deleterious biological effects. In these studies we compare the effects of inorganic arsenicals (arsenite (iAs(III)) and arsenate (iAs(V))) and trivalent and pentavalent methylated arsenicals (methylarsine oxide (MAs(III)O), complex of dimethylarsinous acid with glutathione (DMAs(III)GS), methylarsonic acid (MAs(V)), and dimethylarsinic acid (DMAs(V))) in human keratinocyte cultures. Viability testing showed that the relative toxicities of the arsenicals were as follows: iAs(III) > MAs(III)O > DMAs(III)GS > DMAs(V) > MAs(V) > iAs(V). Trivalent arsenicals induced an increase in cell proliferation at concentrations in the 0.001 to 0.01 microM range, while at high concentrations (>0.5 microM) cell proliferation was inhibited. Pentavalent arsenicals did not stimulate cell proliferation. As seen in the viability studies, the methylated forms of As(V) were more cytotoxic than iAs(V). Exposure to low doses of trivalent arsenicals stimulated secretion of the growth-promoting cytokines, granulocyte macrophage colony stimulating factor and tumor necrosis factor-alpha. DMAs(V) reduced cytokine secretion at concentrations at which proliferation and viability were not affected. These data suggest that methylated arsenicals, products of the metabolic conversion of inorganic arsenic, can significantly affect viability and proliferation of human keratinocytes and modify their secretion of inflammatory and growth-promoting cytokines.
Pseudomonas aeruginosa bacteremia in patients with AIDS.

Twenty-seven episodes of Pseudomonas aeruginosa bacteremia in 21 patients with AIDS were evaluated at the Mount Sinai Medical Center in 1987-1992. Of 21 primary episodes, 12 were acquired in the community, 8 were nosocomial, and one was acquired in a nursing home. Sources of bacteremia (i.e., sites of infection; n = 30) included the lungs (12 cases) an indwelling vascular catheter (9), and the upper respiratory tract (5, including 2 cases of sinusitis, 2 cases of malignant external otitis, and 1 case of epiglottis/pharyngeal cellulitis); in 4 cases the source was unknown. White blood cell counts ranged from 0.1 to 26.2 (mean, 4.32) x 10(3)/mm3; in 19 of 26 cases, the absolute neutrophil count was > 1 x 10(3)/mm3. With the exclusion of primary episodes of bacteremia that resulted in death, the rate of relapse was 33.3% (5 of 15 cases). Mortality for the 25 evaluable episodes of bacteremia was 40% (32% for primary infection and 80% for relapse; P = .06); 52.6% of evaluable patients (10 of 19) ultimately died of P. aeruginosa bacteremia. The institution of appropriate therapy at presentation did not positively affect outcome. Rates of response were higher among episodes treated with a drug combination (an antipseudomonal beta-lactam or monobactam antibiotic plus an aminoglycoside) than among those treated with a single agent (P = .036).