Miracle-dz/UniFAR-FTUs huggingface dataset:
Maturation of cortical circuits requires Semaphorin 7A

Significance Sensory experience exerts profound control over the structure and function of developing cortical circuits during an early postnatal critical period. Abnormalities in this process contribute to perceptual and cognitive deficits, but molecular mechanisms generating excitatory and inhibitory cortical networks during this period remain poorly understood. We show here that Semaphorin 7A (Sema7A) is highly expressed in mouse somatosensory cortex when tactile information conveyed by the thalamus shapes development of somatosensory cortical networks. In mice lacking Sema7A, the anatomical layout of the somatosensory cortex is disrupted, dendritic arbors are misoriented, inhibitory connections develop abnormally, and thalamocortical activity fails to elicit a normal balance of excitation and inhibition. Taken together, our data indicate that maturation of thalamocortical and local circuits in cortex requires Sema7A. Abnormal cortical circuits underlie some cognitive and psychiatric disorders, yet the molecular signals that generate normal cortical networks remain poorly understood. Semaphorin 7A (Sema7A) is an atypical member of the semaphorin family that is GPI-linked, expressed principally postnatally, and enriched in sensory cortex. Significantly, SEMA7A is deleted in individuals with 15q24 microdeletion syndrome, characterized by developmental delay, autism, and sensory perceptual deficits. We studied the role that Sema7A plays in establishing functional cortical circuitry in mouse somatosensory barrel cortex. We found that Sema7A is expressed in spiny stellate cells and GABAergic interneurons and that its absence disrupts barrel cytoarchitecture, reduces asymmetrical orientation of spiny stellate cell dendrites, and functionally impairs thalamocortically evoked synaptic responses, with reduced feed-forward GABAergic inhibition. These data identify Sema7A as a regulator of thalamocortical and local circuit development in layer 4 and provide a molecular handle that can be used to explore the coordinated generation of excitatory and inhibitory cortical circuits.
HSV-2 Serologic Testing in an HMO Population: Uptake and Psychosocial Sequelae

Objectives: To prospectively measure the uptake of Herpes simplex virus Type 2 (HSV-2) testing and psychosocial response to a new serologic diagnosis of HSV-2 in a health maintenance organization (HMO) population. Study Design: Randomly selected urban HMO enrollees were invited to be tested for HSV-2 antibody at a research clinic. Participants had blood drawn and completed demographic and psychosocial questionnaires. Results: Of 3111 eligible enrollees contacted, 344 (11%) were tested. Eighty-seven (26%) tested HSV-2 seropositive, and 44 (51%) of these did not report a prior genital herpes diagnosis. Distress, measured by the total mood disturbance, was 6.5 points higher on average following a new genital herpes diagnosis relative to baseline (actual range = 109 points, P = 0.003) but not statistically different from HSV-2 negative or previously diagnosed participants. Conclusions: HMO enrollees unexpectedly testing HSV-2 positive showed short-term psychosocial distress that resolved during 6-month follow-up. Findings suggest that concerns about psychosocial burden should not deter voluntary serologic HSV-2 testing in primary care settings.
Home Based Entrepreneurship Industry Program to Improve Primary School Teacher Education (PGSD) Students’ Creativity and Innovation

A major challenge for universities today is how to produce graduates who can compete in the global world and can apply their education in society. The government has been encouraging the college students not to be jobseeker but to create jobs. PGSD graduates have been focused on being educators at public or private elementary schools with the status of honorary workers, contract workers, or civil servants (PNS). For graduates who do not get a job, this condition will increase the number of unemployed in Indonesia which is still at the number of 7.01 million people. Responding to this, one solution that can be used as an effort to generate entrepreneurial spirit and overcome the dependence on government in terms of getting a decent job is by encouraging and establishing a home based entrepreneurship program for PGSD students. Home industry is expected to be an alternative income for students, because it is a side business that does not take much time. Through the entrepreneurship program based on home industry, students are expected to become more innovative and creative in creating useful products or services that are useful and valuable in life.
Deposition of complement components on Streptococcus agalactiae in bovine milk in the absence of inflammation

Bovine milk is generally considered to be almost devoid of complement, on the basis of undetectable hemolytic activity, unless inflammation recruits plasma components. This study examines the deposition of complement components on a mastitis-causing isolate of Streptococcus agalactiae by enzyme-linked immunosorbent assay (ELISA). Neat milk from mid-lactating, uninflamed mammary glands (normal milk) effected marked C3 deposition on bacteria. Kinetic studies showed a protracted lag period (30 to 45 min) preceding C3 deposition, which required about 2 h to reach a maximum. Experiments with diluted serum suggested that this slow C3 deposition resulted mainly from the low concentration of certain components of complement in milk. Bacteria incubated in neat milk readily bound bovine conglutinin, indicating the presence of iC3b. Elution of covalently bound C3 fragments with hydroxylamine confirmed the deposition of C3b and iC3b on bacteria. Deposition of C4 on bacteria was not detected in neat milk, suggesting that C3 deposition did not result from the activation of the classical pathway. This was not the result of a lack of antibodies. However, C4 deposition could be obtained by adding purified bovine C1q to normal milk, and C3 deposition was accelerated, suggesting the participation of the classical pathway. The deposition of C1q on antibody-sensitized bacteria was impeded by milk compared with that of C1q diluted in phosphate-buffered saline. Concentrations of C1q in normal milk were very low, ranging from 150 to 250 ng/ml. Overall, these findings indicate that C1q was a limiting factor of the classical pathway in normal milk. The capacity of milk to deposit C3 fragments on mastitis-causing S. agalactiae prompts further studies to investigate its role in opsonophagocytosis.
Carcass and blood components of broiler chickens fed sorghum or millet as replacement for maize in the semi arid zone of Nigeria.

Carcass and blood components of broiler chickens fed sorghum or millet as replacement for maize in the semi arid zone of Nigeria 1Clement Ijaduwa Medugu, 2Ibrahim Dankasa Kwari, 2Joseph Igwebuike, 3Iro Nkama, 3Ibrahim Dukku Mohammed and 4Bruce Hamaker Borno State Agricultural Development Programme (BOSADP) Maiduguri Department of Animal Science, University of Maiduguri, P.M. B. 1069, Maiduguri – Nigeria Department of Food Science and Technology, University of Maiduguri, P.M. B. 1069, Maiduguri – Nigeria Department of Food Science, Purdue University, 1160 Food Science building West Lafayette, IN 47907-1160 ABSTRACT
Change of volatile components in six microalgae with different growth phases.

BACKGROUND Head space solid-phase microextraction-gas chromatography-mass spectrometry has been applied to analyze the volatile components of six marine microalgae (Thalassiosira weissflogii, Nitzschia closterium, Chaetoceros calcitrans, Platymonas helgolandica, Nannochloropsis spp. and Dicrateria inornata) from Bacillariophyta, Chlorophyta and Chrysophyta, respectively, in different growth phases. RESULTS All volatile compounds were identified by database searching in the NIST08 Mass Spectral Library and analyzed by principal component analysis with SIMCA-P software (Umetrics, Umea, Sweden). The results clearly revealed that the volatile components of the six microalgae were significantly different in the exponential, stationary and declining phases. Aldehydes, alkanes, some esters and dimethyl sulfide significantly changed in different growth phases. CONCLUSION This is the first report on the comprehensive characteristics of volatile components in different microalgae and in different growth phases. The results may provide reference data for studies on the flavor of cultivated aquatic organism, odor formation in nature water, choice of feeding period and microalgae species selection for the artificial rearing of marine organisms. © 2016 Society of Chemical Industry.
The Impact of eWOM in Social Media on Consumer Purchase Decisions: A Comparative Study between Romanian and Bosnian Consumers

Marketers define their social media platform as an essential piece to reach their consumers. The flock of web users turning to social media to receive and convey their thoughts, opinions, and suggestions has made social media an integral part of digital marketing. Using traditional media to advertise had restrictions in placement and outcome while, nowadays, social media has liberated this obstacle. It has created a platform which allows information to spread freely and at a fast pace. Using electronic word-of-mouth in social media permits a constant connection to a vast audience. The purpose of this study is to explore the effect of electronic word-of-mouth within the context of social media on consumer purchase decisions. More precisely, how the familiarity of the Electronic Word of Mouth (eWOM) source, the way of communication, writer’s expertise and the popularity of a product affects the consumer's purchase decision. The data has been gathered using quantitative research method. The sample consists of students studying in Sarajevo and Timisoara. Having sample from two different countries would be good base for comparative analysis. The survey for data analysis was emailed to students’ email addresses. The results of the analysis will provide a comparison between the universities and the level of impact eWOM in social media has on their purchase decisions.
Robust depth filter sizing for centrate clarification

Cellulosic depth filters embedded with diatomaceous earth are widely used to remove colloidal cell debris from centrate as a secondary clarification step during the harvest of mammalian cell culture fluid. The high cost associated with process failure in a GMP (Good Manufacturing Practice) environment highlights the need for a robust process scale depth filter sizing that allows for (1) stochastic batch‐to‐batch variations from filter media, bioreactor feed and operation, and (2) systematic scaling differences in average performance between filter sizes and formats. Matched‐lot depth filter media tested at the same conditions with consecutive batches of the same molecule were used to assess the sources and magnitudes of process variability. Depth filter sizing safety factors of 1.2–1.6 allow a filtration process to compensate for random batch‐to‐batch process variations. Matched‐lot depth filter media in four different devices tested simultaneously at the same conditions was used with a common feed to assess scaling effects. All filter devices showed <11% capacity difference and the Pod format devices showed no statistically different capacity differences. © 2015 American Institute of Chemical Engineers Biotechnol. Prog. , 31:1542–1550, 2015
IMPLEMENTASI GOOD CORPORATE GOVERNANCE DI INDONESIA

ABSTRAK Applying good corporate governance becoming become strategic determinant to company so that earning ever improve value and also look after going concern growth process. For the reason, every company requires to continue to improve its hard work so that can take benefit from applying good corporate governance. If this good corporate governance principle is executed seriously, may simply company will have sturdy base in running its business. Externally, company will be more be trusted by investor, meaning its stocks market value will increasingly. Partner work even also does not hesitate to develop broader business relation again. Supplier have trustworthy and clear hold and also sure will be treated fairly so that can give best price, which mean create efficiency to company. Creditors even also have high trust for the giving of its credit which is possible needed to extension of is effort. Keywords : Good corporate governance, fairness, transparency, accountability, and responsibility
Explaining acceptance of e-health services: An extension of TAM and health belief model approach

This study explains factors in terms of intention to use e-health services: consumer informatics e-health type. For this purpose, the original version of Technology Acceptance Model (TAM), which included perceived usefulness, perceived ease of use, and intention to use, is extended with Health Belief Model approach (perceived health risk, health consciousness). A survey method and analyzed data using SmartPLS 3.0 shows that additional factors (perceived health risk, health consciousness) have a significant effect on intention to use e-health services. Perceived health risk and health consciousness exerted strong direct effects on individual perceived usefulness of e-health services. An original construct of TAM, perceived usefulness, also have a strong effect on intention to use e-health services. In contrast, perceived ease of use did not have significant effect on intention to use e-health services.
Intensity of Love in a Community Sample of Spanish Couples in the Region of Madrid

Abstract Intensity of love was examined in a representative community sample of 2,988 couples, from the Region of Madrid (Spain). Gender differences revealed no significant differences in intensity of love. Twenty-five percent of the participants who were in a relationship of less than 10 years and eleven percent of the participants in a relationship of more than 30 years reported being “very intensely in love” F(3, 5.972) = 191.32, p < . 001, ηp 2 = 0.08. The cognitive variables, thinking positively about the partner and thinking about the partner when apart, were significant predictors of the intensity of long-term love for both men and women. Affection was also positively associated with intense love for men (r = .47, p < .001) and women (r = .52, p < .001); sexual intercourse was for women (r = .36, p < .001) but not for men. Wanting to know the whereabouts of partner was associated with the intensity of love for women (r = .15, p < .05), but not for men. Engaging in novel activities with partner was not associated with intense love for male or females. As predicted, love declined significantly in the first decade of marriage, but not much after that. The percentage of men and women who reported being intensely in love was lower than that obtained in the U.S.
The Nordic Housing Enabler: Inter-rater reliability in cross-Nordic occupational therapy practice

Abstract This study addresses development of a content-valid cross-Nordic version of the Housing Enabler and investigation of its inter-rater reliability when used in occupational therapy rating situations, involving occupational therapists, clients, and their home environments. The instrument was translated from the original Swedish version of the Housing Enabler, and adapted according to accessibility norms and guidelines for housing design in Sweden, Denmark, Finland, and Iceland. This iterative process involved occupational therapists, architects, building engineers, and professional translators, resulting in the Nordic Housing Enabler. For reliability testing, the sampling strategy and data collection procedures used were the same in all countries. Twenty voluntary occupational therapists, pair-wise but independently of each other, collected data from 106 cases by means of the Nordic Housing Enabler. Inter-rater reliability was calculated by means of percentage agreement and kappa statistics. Overall good percentage agreement for the personal and environmental components of the instrument was shown, indicating that the instrument was sufficiently reliable for application in practice and research in the Nordic context. The varying kappa results highlight the need for further study in order to understand the influence of prevalence more profoundly, which should be kept in mind when interpreting the results.
Mutations in the multi‐drug resistance regulator MRR1, followed by loss of heterozygosity, are the main cause of MDR1 overexpression in fluconazole‐resistant Candida albicans strains

Overexpression of the MDR1 gene, encoding a multi‐drug efflux pump of the major facilitator superfamily, is a major cause of resistance to the widely used antifungal agent fluconazole and other toxic substances in the fungal pathogen Candida albicans. We found that all tested clinical and in vitro generated C. albicans strains that had become fluconazole‐resistant by constitutive MDR1 upregulation contained mutations in the MRR1 gene, which encodes a transcription factor that controls MDR1 expression. Introduction of the mutated alleles into a drug‐susceptible C. albicans strain resulted in activation of the MDR1 promoter and multi‐drug resistance, confirming that the amino acid substitutions in Mrr1p were gain‐of‐function mutations that rendered the transcription factor constitutively active. The majority of the MDR1 overexpressing strains had become homozygous for the mutated MRR1 alleles, demonstrating that the increased resistance level conferred by two gain‐of‐function alleles provides sufficient advantage to select for the loss of heterozygosity in the presence of fluconazole both in vitro and within the human host during therapy. Loss of heterozygosity usually occurred by mitotic recombination between the two chromosome 3 homologues on which MRR1 is located, but evidence for complete loss of one chromosome and duplication of the chromosome containing the mutated MRR1 allele was also obtained in two in vitro generated fluconazole‐resistant strains. These results demonstrate that gain‐of‐function mutations in MRR1 are the major, if not the sole, mechanism of MDR1 overexpression in fluconazole‐resistant strains and that this transcription factor plays a central role in the development of drug resistance in C. albicans.
Predictors of Clinicopathologic Stage Discrepancy in Oropharyngeal Squamous Cell Carcinoma: A National Cancer Database Study

Objective To determine the frequency, associated factors, and prognosis of clinicopathologic stage discrepancy in oropharyngeal squamous cell carcinoma (OPSCC). Study Design Retrospective study using a national database. Setting National Cancer Database. Subjects and Methods Cases of OPSCC diagnosed between January 1, 2004, and December 31, 2013, with full clinical and pathologic staging information available were identified. Demographic, clinicopathologic, and treatment variables associated with overall stage discrepancy were identified by multivariate logistic regression analysis. Results In total, 7731 cases of OPSCC were identified. Overall stage discrepancy was present in 30.2% of cases (21.9% upstaging, 8.2% downstaging). A total of 13.1% of cases were T-upstaged, and 10.5% of cases were T-downstaged; 22.9% of cases were N-upstaged, and 8.6% of cases were N-downstaged. Upstaging by overall stage was associated with a high Charlson-Deyo score, high tumor grade, number of lymph nodes examined, and increasing tumor size. No factors were positively associated with downstaging. High tumor grade was negatively associated with downstaging. For stage II, III, and IVA tumors, upstaging was associated with poorer OS. Conclusion Clinicopathologic stage discrepancy is common in OPSCC and is likely attributable to insensitive clinical staging techniques as well as to intrinsic tumor biologic properties. Upstaging is associated with poorer prognosis, which is likely due to advancement of disease.
Genetic diversity of HPV16 and HPV18 in Brazilian patients with invasive cervical cancer

Cervical cancer is the fourth most common cancer among women, and ∼70–80% of these cancers are associated with two human papillomavirus types: HPV16 and HPV18. Several studies have reported that intra‐type diversity is associated with the progression of infection to invasive cancer. Herein, we report the genetic diversity of HPV16 and HPV18 in a cohort of 594 Brazilian women with invasive cervical cancer and describe the prevalence of lineages and intra‐type diversity prior to the implementation of the public immunization program in Brazil. HPV detection and genotyping were performed using PCR, PGMY/GP primers, and DNA extracted from fresh tumors. The HPV16 (378 women) and HPV18 (80 women) lineages were identified by PCR and sequencing of the LCR and E6 fragments, followed by SNV comparison and phylogenetic analysis. In our cohort, was found a higher frequency of the lineage A (in 217 women), followed by lineage D (in 97 women) and lineages B and C (in 10 women each) for HPV16; and a higher frequency of lineage A (in 56 women) followed by lineage B (in 15 women) in HPV18. The genetic diversity of HPV16 indicated a recent expansion of specific variants or a selective advantage that is associated with invasive cancer; this pattern was not observed for HPV18. J. Med. Virol. 88:1279–1287, 2016. © 2015 Wiley Periodicals, Inc.
Fuelling Development: Sugarcane Expansion Impacts in Brazil

With the growing effort to decrease greenhouse gas emissions and the inevitable shift towards renewable energy, sugarcane and ethanol production are forecast to expand worldwide. This paper studies the multiple local impacts of the sugar, ethanol and bioelectricity producing mills in the Brazilian state of Mato Grosso do Sul, where the industry grew almost threefold in an eight-year period. We find a myriad of positive impacts related to the entry of sugarcane mills with investments accounting for 130% of the average municipal GDP. Three years after a mill is built, a typical municipality has a 30% increase in GDP; population increases by 10%; employment shoots up by 40%; wages go up 49%; and tax revenues jump 31%. Land use shifts primarily from pastures to sugarcane and there is a decrease in deforestation. There are positive spillovers on agriculture, leading to an increase in the productivity of other crops. Channels contributing to these spillovers include a larger and more educated labor force combined with better financial services, transportation, agricultural equipment and support activities. These results are important to the contentious debate on how different energy sources affect the producing areas. Key-words: Local economic development, spillovers, biofuels, land use. JEL-Codes: R11, R14, Q16 ∗Email: juliano@econ.puc-rio.br. Department of Economics, PUC-Rio, and Climate Policy Initiative. †Email: bpietracci@cpirio.org. Department of Economics, PUC-Rio. ‡Email: prisicila.souza@cpirio.org. Department of Economics, PUC-Rio, and Climate Policy Initiative. 1We acknowledge support from CIFF (Children’s Investment Fund Foundation) and the New Climate Economy to conduct the present research. Breno Pietracci thanks funding from CAPES Postdoctoral Program (PNPD-Capes). We thank Beatriz Ribeiro, Christiane Szerman, Daniel Souza and Giovanna Gottlieb for excellent research assistance.