Miracle-dz/UniFAR-FTUs huggingface dataset:
[The Effectiveness of Cognitive Behavioral Therapy, Medication, or Combined Treatment For Child Hood Anxiety Disorders].

OBJECTIVE The aims of this study were to evaluate the effectiveness of the Fear Hunter cognitive behavioral therapy program, which was developed for the treatment of childhood anxiety disorders, and to compare its effectiveness with standard medication treatment. METHOD A total of 46 participants (aged 8 to 12) that applied to the Ege University, Faculty of Medicine, Child and Adolescent Psychiatry clinic and had a diagnosis of anxiety disorder were recruited for the study. The participants were randomly assigned to cognitive behavioral therapy (CBT), standard drug treatment (ST), or combined treatment (CBT+ ST) groups according to the order of application. Subjects were evaluated using pretest, posttest and 3 months follow-up measurements. The participants were assessed by the researcher using The Screen for Child Anxiety Related Emotional Disorders (SCARED), The Children's Negative Cognitive Errors Questionnaire (CNCEQ), Health Related Quality of Life in Children (Kid-KINDL), and Children's Depression Inventory (CDI). RESULTS The results of repeated measures ANOVA showed that, although general anxiety scores of all treatment conditions significantly decreased at posttest and follow up, a combination of two therapies (CBT+ST) had a significantly superior response rate. Moreover, all treatment conditions including CBT (CBT+ST and CBT) were superior to ST in terms of negative cognitive errors, quality of life, and depression. CONCLUSION It is thought that The Fear Hunter Therapy Program is an effective treatment technique because; it provides significant improvement in the primary and secondary symptoms (e.g. quality of life, depression, negative automatic thoughts) of childhood anxiety disorders.
Epigenetic regulation of metalloproteinases and their inhibitors in rotator cuff tears

Rotator cuff tear is a common orthopedic condition. Metalloproteinases (MMP) and their inhibitors (TIMP) seem to play a role in the development of joint injuries and in the failure of tissue healing. However, the mechanisms of regulation of gene expression in tendons are still unknown. Epigenetic mechanisms, such as DNA methylation and microRNAs regulation, are involved in the dynamic control of gene expression. Here, the mRNA expression and DNA methylation status of MMPs (MMP1, MMP2, MMP3, MMP9, MMP13, and MMP14) and TIMPs (TIMP1-3) and the expression of miR-29 family members in ruptured supraspinatus tendons were compared with non-injured tendons of individuals without this lesion. Additionally, the gene expression and methylation status at the edge of the ruptured tendon were compared with macroscopically non-injured rotator cuff tendon samples from the anterior and posterior regions of patients with tendon tears. Moreover, the possible associations between the molecular alterations and the clinical and histologic characteristics were investigated. Dysregulated expression and DNA methylation of MMP and TIMP genes were found across the rotator cuff tendon samples of patients with supraspinatus tears. These alterations were influenced at least in part by age at surgery, sex, smoking habit, tear size, and duration of symptoms. Alterations in the studied MMP and TIMP genes may contribute to the presence of microcysts, fissures, necrosis, and neovascularization in tendons and may thus be involved in the tendon healing process. In conclusion, MMPs and their inhibitors are regulated by epigenetic modifications and may play a role in rotator cuff tears.
A finite element comparison between the mechanical behaviour of rigid and resilient oral implants with respect to immediate loading

In this paper, a qualitative comparison between two types of dental implants with respect to their behaviour under immediate loading is presented. This analysis has been carried out using the finite element method. Since micromotions (and not the load) are responsible of the appearance of a fibrous interface avoiding osseointegration, the relative displacement between the bone surface and the implant has been the main variable analyzed at different loading states and for the two implant types here considered. The implants analyzed differ in their mechanical behavior: rigid or resilient. Their main difference lies in the joining between the different pieces that make up the dental system. While in the rigid implant all the pieces are screwed, in the resilient implant a relative displacement between the pieces is allowed, with the additional introduction of a silicone gasket that acts like the periodontal ligament. Both implants were considered with a similar geometry and under two different loading scenarios, one equivalent to the force of chewing applied to a molar and another which corresponds to a premolar. For the resilient implant, a hyperelastic behaviour for the silicone and contact conditions between the different mobile parts of the implant are considered. The displacements of the emerging-body in both designs are also compared with the values obtained by several authors. However, the results show that both implants fulfill the constraint of the immediate loading protocol. The micromotions of the resilient implant are lower to those of the rigid one, favouring therefore a good osseointegration process while keeping the stresses in the implant under admissible maximum values.
Development of the endoderm and gut in medaka, Oryzias latipes

We performed an extensive analysis of endodermal development and gut tube morphogenesis in the medaka embryo by histology and in situ hybridization. The markers used in these analyses included sox17, sox32, foxA2, gata‐4, ‐5, ‐6 and shh. sox17, sox32, foxA2, and gata‐5 and ‐6 are expressed in the early endoderm to the onset of gut tube formation. Sections of medaka embryos hybridized with foxA2, a pan‐endodermal marker during gut morphogenesis, demonstrated that gut tube formation is initiated in the anterior portion and that the anterior and mid/posterior gut undergo distinct morphogenetic processes. Tube formation in the anterior endoderm that is fated to the pharynx and esophagus is much delayed and appears to be independent of gut morphogenesis. The overall aspects of medaka gut development are similar to those of zebrafish, except that zebrafish tube formation initiates at both the anterior and posterior portions. Our results therefore describe both molecular and morphological aspects of medaka digestive system development that will be necessary for the characterization of medaka mutants.
Clozapine-Induced Seizures and EEG Abnormalities in Ambulatory Psychiatric Patients

OBJECTIVE: To examine the seizure characteristics and electroencephalogram (EEG) abnormalities in psychiatric patients taking clozapine, given the estimate of a 10% cumulative risk of generalized seizures in this population. DESIGN: We reviewed all consecutive EEGs of ambulatory psychiatric patients taking clozapine performed at our laboratory during 1996 and 1997. SETTING: A university-affiliated urban teaching hospital. SUBJECTS: Twelve patients (4 F/8 M; mean age 40.1 y, range 20–63) had either presented with de novo ictal events within the first month of clozapine therapy (n = 8) or had EEGs recorded to assess seizure risk (n = 4). RESULTS: According to clinical history and interictal EEG findings, the patients were subdivided as follows: three patients with generalized tonic–clonic seizures, two with generalized myoclonic jerks (1 associated with simple partial seizures), two with complex partial seizures, and one with simple partial seizures. The EEGs revealed interictal epileptiform abnormalities (IEDs) in eight patients, two of whom had not had seizures. IEDs were focal or multifocal, with a predominance of left temporal foci. One patient showed a paroxysmal response to photic stimulation. CONCLUSIONS: Patients taking clozapine may be prone to partial seizures and focal EEG abnormalities as well as to generalized seizures and EEG abnormalities, as previously reported.
Surfactant Sodium Lauryl Sulfate Enhances Skin Vaccination

The skin is a highly accessible organ and thus provides an attractive immune environment for cost-effective, simple, and needle-free delivery of vaccines and immunomodulators. In this study, we pretreated mouse skin with an anionic surfactant, sodium lauryl sulfate (SLS), for a short period of time (10 min) followed by epicutaneous vaccination with hen egg lysozyme antigen. We demonstrated for the first time that pretreatment of skin with surfactant SLS significantly enhances the production of antibody to hen egg lysozyme. Short term pretreatment with SLS disorganized the stratum corneum, extracted partial lamellar lipids, induced the maturation of Langerhans cells, and did not result in epidermis thickening. To reveal the mechanism underlying these changes, particularly at the molecular level, we used a novel proteomic technique using ultrafiltration capillaries and mass spectrometry to identify in vivo proteins/peptides secreted in the SLS-pretreated skin. Two secretory proteins, named as calcium-binding protein S100A9 and thymosin β4, were identified by this novel technique. These two proteins thus may provide new insight into the enhancing effect of surfactants on skin vaccination.
Nutritional consequences of small intestinal bacterial overgrowth

15 INTRODUCTION Small intestinal bacterial overgrowth (SIBO) is characterized by a variety of signs and symptoms resulting from nutrient malabsorption when food, enteral feedings, medications such as lactulose or those containing sorbitol or fiber supplements interact with the bacteria. The symptoms may appear minor and nonspecific and lead to diagnostic confusion as these same signs and symptoms are often associated with an underlying disease process when present. SIBO implies a quantitative assessment of bacteria present in the small intestine. Although a certain level of commensal bacteria is important, it is the presence of a particular species type in an atypical location of the bowel, in addition to an excess number, that results in the development of the classical clinical manifestations of this condition. SIBO is usually defined as the presence of >105 colony forming units (cfu)/mL of bacteria in the proximal small intestine (1); however, it has been suggested that a lower colony count (e.g., >103 cfu/mL) may be adequate to induce symptoms as Nutritional Consequences of Small Intestinal Bacterial Overgrowth NUTRITION ISSUES IN GASTROENTEROLOGY, SERIES #69
Crystal structure of bromido-fac-tricarbonyl[5-(3,4,5-trimethoxyphenyl)-3-(pyridin-2-yl)-1H-1,2,4-triazole-κ2 N 2,N 3]rhenium(I) methanol monosolvate

The ReI atom in the title methanol solvate is coordinated octahedrally by two N atoms of the chelating organic ligand, one Br atom and three facially configured carbonyl ligands. Hydrogen bonds between the complex and methanol solvent molecules lead to a layered arrangement in the structure.
Expression of Green Fluorescent Protein Fused to Magnetosome Proteins in Microaerophilic Magnetotactic Bacteria

ABSTRACT The magnetosomes of magnetotactic bacteria are prokaryotic organelles consisting of a magnetite crystal bounded by a phospholipid bilayer that contains a distinct set of proteins with various functions. Because of their unique magnetic and crystalline properties, magnetosome particles are potentially useful as magnetic nanoparticles in a number of applications, which in many cases requires the coupling of functional moieties to the magnetosome membrane. In this work, we studied the use of green fluorescent protein (GFP) as a reporter for the magnetosomal localization and expression of fusion proteins in the microaerophilic Magnetospirillum gryphiswaldense by flow cytometry, fluorescence microscopy, and biochemical analysis. Although optimum conditions for high fluorescence and magnetite synthesis were mutually exclusive, we established oxygen-limited growth conditions, which supported growth, magnetite biomineralization, and GFP fluorophore formation at reasonable rates. Under these optimized conditions, we studied the subcellular localization and expression of the GFP-tagged magnetosome proteins MamC, MamF, and MamG by fluorescence microscopy and immunoblotting. While all fusions specifically localized at the magnetosome membrane, MamC-GFP displayed the strongest expression and fluorescence. MamC-GFP-tagged magnetosomes purified from cells displayed strong fluorescence, which was sensitive to detergents but stable under a wide range of temperature and salt concentrations. In summary, our data demonstrate the use of GFP as a reporter for protein localization under magnetite-forming conditions and the utility of MamC as an anchor for magnetosome-specific display of heterologous gene fusions.
Lexical properties in the writing of foreign language learners over eight years of study : single words and collocations

Lexical proficiency has been defined and researched in terms of lexical knowledge, use and fluency. Different studies have shown that use of vocabulary in a foreign language (or L2) develops more slowly than vocabulary knowledge, either passive or active. However, many studies of free production compared learners of two or three proficiency levels and examined single words, not multiword units, even though the latter are characteristic of idiomatic language, and should be considered a component of lexical use. The data for the present study was collected as part of the on-going compilation of an Israeli learner corpus of written English. The data was analyzed to examine progress in vocabulary use over 8 years of learning, starting with students at the end of elementary school (grade 6) and ending with English majors at the university. The passages were compared on lexical richness – the proportion of frequent to non-frequent vocabulary, on lexical variation – type token ratio, and on the number of collocations. A total of 290 essays (200 words each) were analyzed using the VocabProfile, a software program that calculates the percentage of a text’s words at different frequency levels and provides the text’s type-token ratio. Significant increases in the use of infrequent vocabulary and collocations were found only with the university students. A significant increase in lexical variation was found at the end of high school. The lack of substantial progress during school years, on the one hand, and the significant progress during the one year at university, on the other hand, corroborate previous research. In light of this limited progress, recommendations are made for further investigations into the effect of different pedagogical approaches to the teaching of foreign language vocabulary.
Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats

Aim: The study was designed to investigate the immediate (1 h) effect of leptin and ghrelin injection on in vivo postprandial hepatic glycogen and lipid synthesis. Animals andMethods: Adult Sprague-Dawley male rats were fed a semisynthetic control diet ad libitum. Overnight-fasted rats were gavaged with 4 ml of water containing 1.25 g of the diet and immediately injected intraperitoneally with 4 mCi of 3H2O. After 1 h, rats were either intraperitoneally injected with saline (control), leptin (20 µg/rat) or ghrelin (10 µg/rat) and sacrificed 1 h later. Blood, liver and epididymal fat pads (EFP) were taken for analysis. Results: Plasma triglyceride level was higher in the leptin group compared to control. Leptin injection reduced hepatic glycogen synthesis while glycogen accumulation was not affected and ghrelin injection did not affect hepatic glycogen synthesis. Both hepatic and EFP lipogenesis were not affected by leptin or ghrelin. Conclusion: Leptin and ghrelin administration had no immediate effect on hepatic and adipose tissue lipogenesis. Leptin reduced in vivo postprandial hepatic glycogenesis and increased plasma triglyceride level which may be due to reduced uptake by peripheral tissues. Thus, leptin was found to exert an immediate effect on lipid and carbohydrate metabolism unlike that of ghrelin.
Modern Metal‐on‐metal Hip Resurfacing

&NA; For this technology overview, the tools of evidence‐based medicine were used to summarize information on the indications, effectiveness, and failure rates of modern metal‐on‐metal hip resurfacing technology. The task was complicated by the fact that resurfacing arthroplasty is commonly offered only to a subset of patients who are candidates for total hip replacement, often prohibiting direct comparisons. Comprehensive literature searches were conducted to address four key questions addressing revision rates, patient characteristics, effectiveness of treatment, and whether improved technique, surgeon experience, and/or patient selection lead to improved outcomes. Despite data limitations, it is apparent that revision rates are higher after resurfacing than after total hip arthroplasty. Potential prognostic indicators did not yield a consistent predictor of patient‐oriented outcomes (eg, pain relief) for either resurfacing arthroplasty or total hip replacement. Because of differences between patients who received hip resurfacing and those who received total hip arthroplasty, the results of studies comparing these techniques cannot be interpreted. Finally, changes in technique and increased experience result in a decrease in revision rates and femoral neck fractures and improved pain and hip scores in resurfacing.
Adolescencia e Imagen Corporal en la Época de la Delgadez

Este articulo describe el estado de satisfaccion corporal en un grupo de adolescentes costarricenses de ambos sexos. Se analiza la importancia de la imagen corporal en la adolescencia dentro del contexto de una cultura donde el ser delgado esta estrechamente relacionado con el atractivo fisico. Los y la adolescentes establecen comparaciones entre su apariencia y los modelos ideales sociales, los cuales, al ser poco realistas, les pueden generar insatisfaccion corporal, ansiedad, depresion, baja autoestima y trastornos alimenticios.
KUALITAS PRODUK, HARGA DAN KUALITAS LAYANAN PENGARUHNYA TERHADAP KEPUASAN KONSUMEN PENGGUNA MOBIL NISSAN MARCH PADA PT. WAHANA WIRAWAN MANADO

Kompetisi antar perusahaan saat ini sangat ketat, sehingga manajemen perusahaan berupaya untuk selalu memberikan pelayanan terbaik dengan orientasi penciptaan produk untuk memenuhi kebutuhan dan keinginan pelangganya. Kualitas produk adalah faktor penentu kepuasan konsumen setelah melakukan pembelian dan pemakaian terhadap suatu produk. Tingkat kualitas yang lebih tinggi akan menghasilkan kepuasan pelanggan yang lebih tinggi dan meningkatkan penjualan. Tujuan penelitian ini adalah untuk mengetahui pengaruh kualitas produk, harga dan kualitas layanan terhadap kepuasan konsumen pengguna mobil Nissan March pada PT. Wahana Wirawan Manado. Metode penelitian adalah asosiatif untuk melihat keterhubungan antar variabel. Penelitian ini menggunakan regresi linear berganda . Hasil penelitian yang dilakukan menunjukkan bahwa kualitas produk , harga dan kualitas layanan berpengaruh secara simultan terhadap kepuasan konsumen pengguna mobil Nissan March , sehingga hipotesis yang menyatakan bahwa diduga variabel kualitas produk , harga dan kualitas layanan secara bersama berpengaruh terhadap kepuasan konsumen dapat di terima. Pihak manajemen, sebaiknya memperhatikan harga mengingat koefisien harga dalam penelitian ini lemah. Kata kunci : kualitas produk, harga, kualitas layanan, kepuasan konsumen
Home IVIG for CIDP: A Focus on Patient Centred Care

Objective: To determine the safety and tolerability of home-based intravenous immunoglobulin (IVIG) (Gamunex) as maintenance treatment in patients with chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) in Canada. Methods: We enrolled ten subjects with CIDP who had previously received IVIG in the hospital setting to receive the comparable IVIG dose (1-2 g/kg/month) in the home for six months. The patients were evaluated in the clinic at three months and at six months to evaluate their clinical status as well as the safety and tolerability of IVIG. Results: All subjects tolerated home-based IVIG treatment as maintenance treatment of CIDP. There were no serious adverse events related to IVIG. Subjects did experience “anticipated” IVIG events post-infusion such as headache and fatigue, which were managed with analgesics and supportive counseling. One subject withdrew consent at end of study due to hospitalization. This event was not related to the IVIG. Another subject experienced a “flare” of CIDP symptoms near the end of the study, however, completed all visits as per protocol. All subjects expressed excellent satisfaction with the individualized therapy, and almost all (nine out of ten) patients preferred home-infusion to hospital-infusion. Conclusion: Intravenous immunoglobulin can be delivered safely and is well tolerated outside the hospital setting in Canada in patients with chronic, stable neuromuscular conditions such as CIDP who have previously tolerated IVIG in the hospital medical day Care Unit.
Recent Advances in the Treatment of Chronic Refractory Immune Thrombocytopenic Purpura

We define chronic refractory immune thrombocytopenic purpura (ITP) as ITP with persistent thrombocytopenia following treatment with glucocorticoids and splenectomy. Chronic refractory ITP is uncommon, occurring in fewer than 10% of all adult patients with ITP diagnoses.The goal of treatment is only to achieve a safe platelet count with minimal treatment-related risk. A safe platelet count may be considered to be as low as 10,000/μL, because the risk for major bleeding in otherwise healthy subjects is great only when the platelet count is less than 10,000/μL. Observation without specific treatment is appropriate for patients with moderate thrombocytopenia and no clinically important bleeding symptoms. For patients with chronic refractory ITP who require treatment, there is no consensus for what therapies to use or the sequence in which to use them. For patients with severe and symptomatic thrombocytopenia, the use of anti-CD20 (rituximab) and immunosuppressive agents, alone or in combination, may be most effective.The mechanism of all current therapies is to decrease the accelerated platelet destruction brought about by immunosuppression. An alternative approach, the stimulation of platelet production with thrombopoietic agents, has been successful in investigational studies and may provide a new management option.
CEBP factors regulate telomerase reverse transcriptase promoter activity in whey acidic protein‐T mice during mammary carcinogenesis

Telomerase is activated in the majority of invasive breast cancers, but the time point of telomerase activation during mammary carcinogenesis is not clear. We have recently presented a transgenic mouse model to study human telomerase reverse transcriptase (TERT) gene expression in vivo (hTERTp‐lacZ). In the present study, hTERTp‐lacZxWAP‐T bitransgenic mice were generated to analyze the mechanisms responsible for human and mouse TERT upregulation during tumor progression in vivo. We found that telomerase activity and TERT expression were consistently upregulated in SV40‐induced invasive mammary tumors compared to normal and hyperplastic tissues and ductal carcinoma in situ (DCIS). Human and mouse TERT genes are regulated similarly in the breast tissue, involving the CEBP transcription factors. Loss of CEBP‐α and induction of CEBP‐β expression correlated well with the activation of TERT expression in mouse mammary tumors. Transfection of CEBP‐α into human or murine cells resulted in TERT repression, whereas knockdown of CEBP‐α in primary human mammary epithelial cells resulted in reactivation of endogenous TERT expression and telomerase activity. Conversely, ectopic expression of CEBP‐β activated endogenous TERT gene expression. Moreover, ChIP and EMSA experiments revealed binding of CEBP‐α and CEBP‐β to human TERT‐promoter. This is the first evidence indicating that CEBP‐α and CEBP‐β are involved in TERT gene regulation during carcinogenesis.