Miracle-dz/UniFAR-FTUs huggingface dataset:
Metipranolol promotes structure and function of retinal photoreceptors in the rd10 mouse model of human retinitis pigmentosa

Metipranolol is a β‐adrenergic receptor antagonist that is given orally for the treatment of hypertension and also applied topically to the cornea for treating glaucoma. It also inhibits nitrosative stress which has previously been shown to be the cause of cone photoreceptor death in retinitis pigmentosa. In this study, we tested the hypothesis that metipranolol protects photoreceptor structure and function in the mouse model rd10. At P35, compared with vehicle‐treated rd10 mice in which rod degeneration was nearly complete, rd10 mice given daily subcutaneous injections of 40 mg/kg of metipranolol had reduction in markers of nitrosative stress, fewer TUNEL‐positive cells, increased outer nuclear layer thickness, and substantially more staining for rhodopsin. This was accompanied by significantly higher mean scotopic and photopic electroretinogram b‐wave amplitudes indicating improved photoreceptor function. At P50, metipranolol‐treated rd10 mice had decreased 3‐nitrotyrosine staining in the retina, increased immunostaining for cone arrestin, a marker for cone photoreceptors, and significantly higher scotopic and photopic b‐wave amplitudes at the highest stimulus intensity compared with vehicle‐treated mice. At P65, cone density was significantly higher in metipranolol‐treated versus vehicle‐injected rd10 mice. Metipranolol applied as eye drops promoted cone photoreceptor function in retinas of rd10 mice greater than subcutaneously injected metipranolol. The reduced nitrosative damage and rescue of functional loss of photoreceptors in rd10 mice suggests that metipranolol, a drug with established ocular safety and tolerability, may have potential for treating patients with retinitis pigmentosa.
Number of Daily Peritoneal Dialysis Exchanges and Mortality Risk in a Chinese Population

Background: We report outcomes on ≥ 4 compared with < 4 exchanges/day in a Chinese cohort on continuous ambulatory peritoneal dialysis (CAPD). Methods: Data were sourced from the Baxter (China) Investment Co. Ltd Patient Support Program database, comprising an inception cohort commencing CAPD between 1 January 2005 and 13 August 2015. We used cause-specific Cox proportional hazards and Fine-Gray competing risks (kidney transplantation, change to hemodialysis) models to estimate mortality risk on ≥ 4 compared with < 4 exchanges/day. We matched or adjusted for age, gender, employment, insurance, primary renal disease, size of CAPD program, year of dialysis inception, and treatment center. Results: We modeled 100,022 subjects from 1,177 centers over 239,876 patient-years. Of these subjects, 43,185 received < 4 exchanges/day and 56,837 ≥ 4 exchanges/day. The proportion of patients on < 4 exchanges/day varied widely between centers. Those on < 4 exchanges/day were significantly older, more often female, of unknown employment, and from rural China. In the various models, ≥ 4 exchanges/day was associated with a significantly lower risk of death by 30% – 35% compared with < 4 exchanges/day. This beneficial effect was greatest in younger and rural patients. Conclusions: In this Chinese CAPD cohort, ≥ 4 exchanges/day was associated with significantly lower mortality risk than < 4 exchanges/day. Analyses are limited by residual confounding from unavailability of important prognostic covariates (e.g., comorbidity, socioeconomic factors) and data on residual renal function, peritoneal clearance, and transport status with which to judge the clinical appropriateness of CAPD prescription. Nonetheless, our study indicates this area as a high priority for further detailed study.
Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor

The Bacille Calmette-Guérin (BCG) vaccine comprises a family of genetically different strains derived by the loss of genomic regions (RDs) and other mutations. In BCG Moreau, loss of RD16 inactivates rv3405c * , encoding a transcriptional repressor that negatively regulates the expression of Rv3406, an alkyl sulfatase. To evaluate the impact of this loss on the BCG and host cell viability and the cytokine profile, THP-1 cells were infected with BCG Moreau (harbouring the empty vector) and a complemented strain carrying a functional copy of rv3405c. Viability of the host cells and bacteria as well as the pattern of cytokine secretion were evaluated. Our results show that the viability of BCG Moreau is higher than that of the complemented strain in an axenic medium, suggesting a possible functional gain associated with the constitutive expression of Rv3406. Viability of the host cells did not vary significantly between recombinant strains, but differences in the profiles of the cytokine secretion (IL-1β and IL-6) were observed. Our results suggest an example of a functional gain due to gene loss contributing to the elucidation of the impact of RD16 on the physiology of BCG Moreau.
EWSR1 Rearrangement and CD99 Expression as Diagnostic Biomarkers for Ewing/PNET Sarcomas in a Moroccan Population

Ewing sarcoma/primitive neuroectodermal tumor (Ewing/PNET sarcomas or EPS) are a group of round cell tumors. Malignant round cell tumors form a large and diverse group that includes rhabdomyosarcoma, synovial sarcoma, non-Hodgkin's lymphoma, neuroblastoma, hepatoblastoma, Wilm's tumor, desmoplastic small round cell tumor, and other morphologically similar entities. Differential diagnosis of Ewing sarcoma/primitive neuroectodermal tumor (Ewing/PNET sarcomas or EPS) is difficult. In addition to morphology and immunohistochemistry (IHC), differential diagnosis of these tumors is based on molecular analysis of the EWSR1 gene rearrangement using fluorescent in situ hybridization (FISH) technique. We investigated the diagnostic value of combined CD99 immunostaining and EWSR1 t(22q12) alteration using a dual-color, break-apart rearrangement probe in forty-one formalin-fixed paraffin-embedded (FFPE) tissue samples from pediatric and adult patients diagnosed with EPS. IHC was performed in all cases using the CD99 antibody and showed a positivity of 92.7% in the enrolled cases (38/41) followed by FISH analysis where 48.8% of the cases (20/41) were rearranged. Sensitivity and specificity for IHC assays were 88% and 58%, respectively. Notably, FISH had a sensitivity of 100% and a specificity of 87%. In addition, CD99 positivity was found to correlate with EWSR1 rearrangement (p < 0.05). This report shows that FISH has better sensitivity and specificity than IHC in the Moroccan population, and supports its combination with CD99 immunostaining as diagnostic biomarkers for this rare malignant entity. ”
Exploring factors for effective use of online information in SPOC within the engineering education

Today's Small Private Online Course (SPOC) environments are mashing different services from public, private, or hybrid clouds, different equipment, different tools, platforms, and learning pedagogies. The aim of this paper is to provide designers, developers, and educators with insights for implementation and utilization of such complex and heterogeneous SPOC frameworks. Factors that impact effective use of online information of designed digital content for engineering courses and successfulness of such systems are examined using SPOC platform at university course. First outcome of this study is fact that learners have used and accepted SPOC platform positively, which is examined using questionnaire. Second, this study as a result identified following important drivers for SPOC platform successfulness: the high quality of information and useful learning contents, easy for use, and social presence support. These drivers lead to learners feel usefulness of the platform and to enjoy in using it. Study also shown that SPOC platform quality is strong related to learners’ perceptions with the system and perceived easy of use.
The GreedEx experience: Evolution of different versions for the learning of greedy algorithms

This paper presents the evolution experienced by GreedEx, an interactive application to learn greedy algorithms. It shows the four different versions currently available. A first original version for computers, two versions for iPad, and another version for smartphones. Besides, it describes the evaluation performed for each version, which has served so as to justify such evolution.
AIM‐Mobile Learning Platform to enhance the teaching‐learning process using smartphones

We developed the responsive environment AIM‐Mobile Learning Platform, which permits to assess students using smartphones and to design online educational packets. We carried out a qualitative and quantitative study in Higher Education subjects using the case study and the triangulation data methods. Results demonstrated that the environment promotes a dynamic learning‐teaching process inside class, increasing the motivation, participation, and attention of students and helping them to be more conscious of their self‐learning process.
Spread: a new layer for profiled deep-learning side-channel attacks

Recent publications, such as [10] and [13], exploit the advantages of deep-learning techniques in performing Side-Channel Attacks. One example of the Side-Channel community interest for such techniques is the release of the public ASCAD database, which provides power consumption traces of a masked 128-bit AES implementation, and is meant to be a common benchmark to compare deep-learning techniques performances. In this paper, we propose two ways of improving the effectiveness of such attacks. The first one is as new kind of layer for neural networks, called “Spread” layer, which is efficient at tackling side-channel attacks issues, since it reduces the number of layers required and speeds up the learning phase. Our second proposal is an efficient way to correct the neural network predictions, based on its confusion matrix. We have validated both methods on ASCAD database, and conclude that they reduce the number of traces required to succeed attacks. In this article, we show their effectiveness for first-order and second-order attacks.
Multiplicative Masking for AES in Hardware

Hardware masked AES designs usually rely on Boolean masking and perform the computation of the S-box using the tower-field decomposition. On the other hand, splitting sensitive variables in a multiplicative way is more amenable for the computation of the AES S-box, as noted by Akkar and Giraud. However, multiplicative masking needs to be implemented carefully not to be vulnerable to first-order DPA with a zero-value power model. Up to now, sound higher-order multiplicative masking schemes have been implemented only in software. In this work, we demonstrate the first hardware implementation of AES using multiplicative masks. The method is tailored to be secure even if the underlying gates are not ideal and glitches occur in the circuit. We detail the design process of first- and second-order secure AES-128 cores, which result in the smallest die area to date among previous state-of-the-art masked AES implementations with comparable randomness cost and latency. The first- and second-order masked implementations improve resp. 29% and 18% over these designs. We deploy our construction on a Spartan-6 FPGA and perform a side-channel evaluation. No leakage is detected with up to 50 million traces for both our first- and second-order implementation. For the latter, this holds both for univariate and bivariate analysis.
Occult uterine malignancy during laparoscopic supracervical hysterectomy.

OBJECTIVES To estimate the incidence of occult uterine malignancies during laparoscopic supracervical hysterectomy (LSH). MATERIAL AND METHODS Retrospective cohort study based on archival data (2010-2016) of the Department of Gynecology and Oncology, Jagiellonian University. RESULTS Medical records of 696 women, who underwent LSH were analyzed. Two occult sarcomas (2/696; 0.29%, 0.003, 95% CI: 0.001 to 0.01), including one case of low-grade endometrial stromal sarcoma (ESS) with co-occurring atypical endometrial hyperplasia (AH) and one case of high-grade ESS were found postoperatively. One case of invasive primary fallopian tube cancer (1/696; 0.14%, 0.001, 95% CI: 0.00 to 0.008) and additional three cases of AH (3/696; 0.57%, 0.004, 95% CI: 0.001 to 0.013) were also identified. No case of EC was documented. One hundred sixty nine (24.3%) women of 696 had an endometrial sampling prior LSH including these with ESS. We did not observe worsening of the prognosis and all patients with confirmed malignancy are still alive and free from recurrence in 2-5 years of observations. CONCLUSIONS Most commonly the occult malignancy would have not been recognized if the surgery had not been conducted. When appropriate diagnostics is conducted, rare incidents of malignant tissue morcellation should not be considered as a professional misconduct but as a possible adverse event. Patients should be informed about the risk of malignancy according to available estimations and that endometrial sampling cannot eliminate such a risk. A consensus regarding safe indications, required diagnostics, and justifiability of mandatory use of contained morcellation for LSH should be developed.
LncRNA882 regulates leukemia inhibitory factor (LIF) by sponging miR‐15b in the endometrial epithelium cells of dairy goat

Despite the fact that long noncoding RNAs (lncRNAs) play roles in almost all biological processes, little is known about their biological function in the endometrium during the formation of endometrial receptivity. In this study, a comprehensive analysis of lncRNAs in goat endometrial tissues on Day 5 (prereceptive endometrium, PE) and Day 15 (receptive endometrium, RE) of pregnancy was performed by using RNA‐Seq. As a result, 668 differentially expressed lncRNAs (DELs) were found between the PE and RE. Further study showed that lncRNA882, regulated by estrogen (E2) and progestin (P4), could act as competing endogenous RNAs (ceRNAs) for miR‐15b, which inhibited the expression of transforming growth factor‐b‐activated kinase 1 binding protein 3 (TAB3) and then indirectly regulated the level of leukemia inhibitory factor (LIF). This was helpful for the formation of endometrial receptivity in dairy goats. In conclusion, we elucidated the endometrium lncRNA profiles of PE and RE in dairy goats; lncRNA882 acted as a ceRNA for miR‐15b and then indirectly regulated the level of LIF in goat endometrial epithelium cells. Thus, this study helped us to better understand the molecular regulation of endometrial receptivity in dairy goats.
The mechanisms of DIRAS family members in role of tumor suppressor

DIRAS family is a group of GTPases belonging to the RAS superfamily and shares homology with the pro‐oncogenic Ras GTPases. Currently, accumulating evidence show that DIRAS family members could be identified as putative tumor suppressors in various cancers. The either lost or reduced expression of DIRAS proteins play an important role in cancer development, including cell growth, migration, apoptosis, autophagic cell death, and tumor dormancy. This review focuses on the latest research regarding the roles and mechanisms of the DIRAS family members in regulating Ras function, cancer development, assessing potential challenges, and providing insights into the possibility of targeting them for therapeutic use.
Environment-dependent attack rates of cryptic and aposematic butterflies

Abstract Many organisms have evolved adaptive coloration that reduces their risk of predation. Cryptic coloration reduces the likelihood of detection/recognition by potential predators, while warning or aposematic coloration advertises unprofitability and thereby reduces the likelihood of attack. Although some studies show that aposematic coloration functions better at decreasing attack rate than crypsis, recent work has suggested and demonstrated that crypsis and aposematism are both successful strategies for avoiding predation. Furthermore, the visual environment (e.g., ambient lighting, background) affects the ability for predators to detect prey. We investigated these 2 related hypotheses using 2 well-known visually aposematic species of Heliconius butterflies, which occupy different habitats (open-canopy vs. closed-canopy), and one palatable, cryptic, generalist species Junonia coenia. We tested if the differently colored butterflies differ in attack rates by placing plasticine models of each of the 3 species in 2 different tropical habitats where the butterflies naturally occur: disturbed, open-canopy habitat and forested, closed-canopy habitat. The cryptic model had fewer attacks than one of the aposematic models. Predation rates differed between the 2 habitats, with the open habitat having much higher predation. However, we did not find an interaction between species and habitat type, which is perplexing due to the different aposematic phenotypes naturally occurring in different habitats. Our findings suggest that during the Panamanian dry season avian predation on perched butterflies is not a leading cause in habitat segregation between the 2 aposematic species and demonstrate that cryptically colored animals at rest may be better than aposematic prey at avoiding avian attacks in certain environments.
Ownershift: Facilitating Overhead Interaction in Virtual Reality with an Ownership-Preserving Hand Space Shift

We present Ownershift, an interaction technique for easing overhead manipulation in virtual reality, while preserving the illusion that the virtual hand is the user's own hand. In contrast to previous approaches, this technique does not alter the mapping of the virtual hand position for initial reaching movements towards the target. Instead, the virtual hand space is only shifted gradually if interaction with the overhead target requires an extended amount of time. While users perceive their virtual hand as operating overhead, their physical hand moves gradually to a less strained position at waist level. We evaluated the technique in a user study and show that Ownershift significantly reduces the physical strain of overhead interactions, while only slightly reducing task performance and the sense of body ownership of the virtual hand.
Phospholipase C‐γ2 promotes intracellular survival of mycobacteria

Mycobacterium tuberculosis (Mtb) infects millions of people each year. These bacilli can survive inside macrophages. To favor their survival, pathogen alters various signal transduction pathways in host cells. Phospholipase C (PLC) signaling regulates various processes in mammalian cells but has never been investigated for their roles in regulating phagocytosis and killing of mycobacteria by macrophages. Here, we report that infection with Mtb but not Mycobacterium smegmatis (MS) induces phosphorylation of PLC‐γ2 at tyrosine 1217 in J774A.1 cells. Small interfering RNA–mediated knockdown of PLC‐γ2 expression leads to the enhanced killing of both MS and Mtb by these cells suggesting that Mtb activates PLC‐γ2 to promote its intracellular survival within macrophages. Knockdown of PLC‐γ2 also lead to increased uptake of Mtb but not MS by J774.A. 1 cells. Further, we have observed that PLC‐γ2 was required for Mtb‐induced inhibition of expression of proinflammatory cytokine tumor necrosis factor‐α, inducible nitric oxide synthase, and chemokine (C‐C motif) ligand 5 (RANTES). Altogether, our results for the first time demonstrate that Mtb induces activation of macrophages PLC‐γ2 to inhibit their mycobactericidal response.
The Usefulness of Immunocytochemistry of CD56 in Determining Malignancy from Indeterminate Thyroid Fine-Needle Aspiration Cytology

Background Fine-needle aspiration cytology serves as a safe, economical tool in evaluating thyroid nodules. However, about 30% of the samples are categorized as indeterminate. Hence, many immunocytochemistry markers have been studied, but there has not been a single outstanding marker. We studied the efficacy of CD56 with human bone marrow endothelial cell marker-1 (HBME-1) in diagnosis in the Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) category III. Methods We reviewed ThinPrep liquid-based cytology (LBC) samples with Papanicolaou stain from July 1 to December 31, 2016 (2,195 cases) and selected TBSRTC category III cases (n = 363). Twenty-six cases were histologically confirmed as benign (six cases, 23%) or malignant (20 cases, 77%); we stained 26 LBC slides with HBME-1 and CD56 through the cell transfer method. For evaluation of reactivity of immunocytochemistry, we chose atypical follicular cell clusters. Results CD56 was not reactive in 18 of 20 cases (90%) of malignant nodules and showed cytoplasmic positivity in five of six cases (83%) of benign nodules. CD56 showed high sensitivity (90.0%) and relatively low specificity (83.3%) in detecting malignancy (p = .004). HBME-1 was reactive in 17 of 20 cases (85%) of malignant nodules and was not reactive in five of six cases (83%) of benign nodules. HBME-1 showed slightly lower sensitivity (85.0%) than CD56. The specificity in detecting malignancy by HBME-1 was similar to that of CD56 (83.3%, p = .008). CD56 and HBME-1 tests combined showed lower sensitivity (75.0% vs 90%) and higher specificity (93.8% vs 83.3%) in detecting malignancy compared to using CD56 alone. Conclusions Using CD56 alone showed relatively low specificity despite high sensitivity for detecting malignancy. Combining CD56 with HBME-1 could increase the specificity. Thus, we suggest that CD56 could be a useful preoperative marker for differential diagnosis of TBSRTC category III samples.
Phenotypic and genotypic characterization of Candida species isolated from candideamia in Iran

Background and Purpose: Candidemia is one of the most important fungal infections caused by Candida species. Infections and mortality caused by Candida species have been on a growing trend during the past two decades. The resistance of yeasts to antifungal drugs and their epidemiological issues have highlighted the importance of accurately distinguishing the yeasts at the species level. The technique applied for yeast identification should be fast enough to facilitate the imminent initiation of the appropriate therapy. Candidemia has not been studied comprehensively in Iran yet. Regarding this, the current study aimed to assess the epidemiology of candidemia at Tehran hospitals and compare the results with the previous ﬁndings. Materials and Methods: This study was conducted on 204 positive blood cultures obtained from 125 patients hospitalized in several hospitals located in Tehran, Iran, within a period of 13 months. The yeast isolation and species identification were accomplished using several phenotypic methods (i.e., production of germ tube in human serum, culture on CHROMagar Candida, and Corn meal agar containing Tween 80) and molecular methods, such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In addition, unknown cases were subjected to PCR sequencing. These methods were then compared in terms of accuracy, sensitivity, and speed of identiﬁcation. Results: According to the results, C. albicans (62.4%) was the most common isolate, followed by C. parapsilosis (n=36, 17.5%), C. glabrata (n=18, 8.8%), C. tropicalis (n=13, 6.3%), Trichosporon asahii (n=3, 1.5%), C. kefyr (n=2, 1.0%), C. lusitaniae (n=2, 1.0%), C. intermedia (n=1, 0.5%), C. guilliermondii (n=1, 0.5%), and C. krusei (n=1, 0.5%), respectively. Conclusion: As the findings indicated, the most common species causing candidemia were C. albicans, C. parapsilosis, and C. glabrata, respectively. Children less than one year old and people with cancer were at higher risk for candidemia, compared to other groups. Moreover, phenotypic and molecular methods resulted in the identification of 65.2% and 96.6% of the isolates, respectively. Consequently, PCR-RFLP could be concluded as a more favorable technique for species identification.