Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of progesterone prior to GnRH-PGF2alpha treatment on induction of oestrus and pregnancy in anoestrous Awassi ewes.

An experiment was conducted to examine the effect of progesterone prior to a GnRH-PGF2alpha treatment on oestrus and pregnancy in seasonally anoestrous Awassi ewes. Twenty-four ewes were randomly assigned to three groups to be pre-treated with 60 mg medroxyprogesterone acetate sponges (group A), 600 mg progesterone sponges (group B) or blank sponges (group C) for 4 days. All ewes were injected with 100 microg of GnRH 24 h after sponge removal followed, 5 days later, by 20 mg PGF2alpha injection. Ewes were exposed to three fertile rams at the time of PGF2alpha injection (day 0, 0 h) and were checked for breeding marks at 6-h intervals for 5 days. Blood samples were collected from all ewes 1 day (day -10) prior to sponge insertion, at the time of sponge removal (day -6), 1 day following sponge removal (day -5, at the time of GnRH injection) and at the time of PGF2alpha injection (day 0) for analysis of progesterone. Progesterone concentrations on days -10 and -5 were basal and averaged 0.2 +/- 0.04 and 0.2 +/- 0.2 ng/ml, respectively. Progesterone concentrations on day -6 were elevated only in group B ewes and were higher (p < 0.0001) than those of groups A and C. Progesterone concentrations on day 0 were higher (p = 0.002) in groups A and B than group C. Oestrous responses occurred only in ewes of groups A and B (p > 0.05). Induced oestrus conception rate was greater (p < 0.01) in group A than groups B and C. Ewes returned to oestrus 17-20 days following day 0 were two of eight, six of eight and three of eight of groups A, B and C, respectively, all of which eventually lambed. The overall lambing rate was 82% in progesterone-primed ewes compared with only 38% non-progesterone-primed ewes (p < 0.05). Progesterone priming apparently sensitizes GnRH-PGF2alpha-treated seasonally anoestrous ewes and increases their response in oestrus and pregnancy rates.
The effects of handholding on anxiety in cataract surgery patients under local anaesthesia.

AIM OF THE STUDY To assess the effectiveness of handholding on the anxiety of patients undergoing planned cataract surgery under local anaesthesia. BACKGROUND Anxiety concerning anaesthesia, pain, physical injuries, isolation, prognosis, possibilities of deformity, or loss of self-control may be stressful to patients undergoing surgery. Anxiety activates the sympathetic nervous system, characterized by an increase in catecholamine concentration, heart rate and blood pressure and increased glucocorticoid levels; it also affects immune responses. Therefore, there is a need for nursing interventions to reduce the anxiety of patients under local anaesthesia who are conscious. METHODS An untreated control group design with pre and post-test was used. Among 62 patients, 30 were randomly assigned to the handholding group and 32 to the control group. Handholding was provided to subjects of the handholding group during surgery. Visual analogue scales and interviews were used to measure anxiety, and pulse rate and systolic and diastolic blood pressure were used as physiological measures of stress. Blood was taken for analysis of levels of epinephrine, norepinephrine, cortisol, neutrophils, lymphocytes and natural killer cells. RESULTS The number of subjects who reported decreased anxiety during operation was significantly higher in the handholding group compared with the control group and most of the subjects reported that handholding during operation was very helpful in reducing anxiety. Epinephrine levels in the handholding group were significantly lower than in the control group. CONCLUSIONS Results suggest that this noninvasive intervention has potential for reducing anxiety in patients having cataract surgery under local anaesthesia.
Manganese Efficiency in Barley: Identification and Characterization of the Metal Ion Transporter HvIRT11[OA]

Manganese (Mn) deficiency is an important plant nutritional disorder in many parts of the world. Barley (Hordeum vulgare) genotypes differ considerably in their ability to grow in soils with low Mn2+ availability. Differential genotypic Mn efficiency can be attributed to differences in Mn2+ uptake kinetics in the low nanomolar concentration range. However, the molecular basis for these differences has not yet been clarified. We present here the identification and characterization of the first barley gene encoding a plasma membrane-localized metal transport protein able to transport Mn2+. The gene is designated HvIRT1 (for IRON-REGULATED TRANSPORTER1) because it belongs to the ZIP gene family and has a high similarity to rice (Oryza sativa) OsIRT1. A novel yeast uptake assay based on inductively coupled plasma-mass spectrometry analysis of 31 different metal and metalloid ions showed that the HvIRT1 protein, in addition to Mn2+, also transported Fe2+/Fe3+, Zn2+, and Cd2+. Both Mn and iron deficiency induced an up-regulation of HvIRT1 in two barley genotypes differing in Mn efficiency, but the expression levels in all cases were highest (up to 40%) in the Mn-efficient genotype. The higher expression of HvIRT1 correlated with an increased Mn2+ uptake rate. We conclude that HvIRT1 is an important component controlling Mn2+ uptake in barley roots and contributes to genotypic differences in Mn2+ uptake kinetics.
The Body in Performance

Abramovic, Marina and Pijnappel, Johan 1995. Marina Abramovic: cleaning the house. London: Academy Editions. Anderson, P. 2010. So much wasted: hunger, performance, and the morbidity of resistance. Durham, N.C. : Duke University Press. Anderson, P. and Menon, J. 2009. Violence performed: local roots and global routes of conflict. Basingstoke: Palgrave Macmillan. Anker, V. 2008. Digital dance: the effects of interaction between new technologies and dance performance. Saarbrucken: VDM Verlag Dr. Muller. Anon, 2007. Marina Abramovic: 7 easy pieces. Milan: Edizioni Charta. Anon, 2010. Understanding Pina: the legacy of Pina Bausch. Anon, n.d. Activism and performance. Anon, n.d. After the fall: dance-theatre and dance-performance. In: Contemporary Theatres in Europe. [online] Routledge, pp.188–198. Available at: . Anon, n.d. Belarus Free Theatre | Theatre group. Available at: . Anon, n.d. CIRCA The Clandestine Insurgent Rebel Clown Army. Available at: . Anon, n.d. Coco Fusco. Available at: . Anon, n.d. DeDomenici Temporary Website. Available at: . Anon, n.d. Extremism and performance. Anon, n.d. Home | DV8. Available at: . Anon, n.d. Human rights and performance. Anon, n.d. In Place of War. Available at: . Anon, n.d. Interdisciplinarity and performance. Anon, n.d. Introduction. Anon, n.d. Kleinkunst and performance. Anon, n.d. Peformance Studies. Available at: . Anon, n.d. Performance and audience. Anon, n.d. Platform. Available at: . Anon, n.d. Questions to consider: Anon, n.d. Questions to consider: Anon, n.d. Questions to consider: Anon, n.d. Questions to consider: Anon, n.d. Questions to consider: . Anon, n.d. Questions to consider: . Anon, n.d. Questions to consider: . Anon, n.d. Reverend Billy & The Church of Stop Shopping. Available at: . Anon, n.d. Tanztheater: a dancing across margins. In: Liminal acts. [online] London: Cassell. Available at: . Anon, n.d. The atlas group. Available at: . Anon, n.d. The ontology of performance: representation without reproduction. In: Unmarked: the politics of performance. [online] London: Routledge. Available at: . Anon, n.d. The politics of liveness and the body in performance. Anon, n.d. The Universal Declaration of Human Rights. Available at: . Anon, n.d. Theatre of the Oppressed. Available at: . Anon, n.d. Tricycle Theatre. Available at: . Anon, n.d. UbuWeb Dance. Available at: . Anon, n.d. the vacuum cleaner an artist and activist collective of one. Available at: . Ashery, Oreet 2007. Oreet Ashery. Athey, Ron and Adams, Lee 2009. Visions of excess.
The relationship between transformational leadership and organizational learning culture in magnet and non-magnet hospitals

The purpose of this study was to determine the relationship between transformational leadership and organizational learning culture in perioperative nursing units, and further, whether there was a difference in these relationships between hospitals with and without ANCC Magnet Recognition ®. The study also explored whether there were significant differences between perioperative staff nurse perceptions of leadership and learning culture in Magnet and nonMagnet hospitals. Using a quantitative, correlational design, this study measured the responses of 1,121, board certified perioperative nurses to a survey comprised of items from the Multifactor Leadership Questionnaire (MLQ5X) (Avolio & Bass, 1995), the Dimensions of Learning Organization Questionnaire (DLOQ-A) (Watkins 1993; 1996), and select demographic questions including whether or not their hospital had earned the Magnet designation. Regression analysis and ANOVA findings indicated a strong, positive correlation between transformational leadership and organizational learning culture. This relationship persisted after adjusting for demographics and hospital characteristics. However, Magnet status did not have a moderating effect on this relationship. While Magnet hospitals had significantly higher total scores for both transformational leadership and learning culture than non-Magnet hospitals, the relationship between transformational leadership and learning culture was similar in both groups. These findings reinforce the beneficial effects of the ANCC Magnet Recognition Program ® on nursing work environments, which have been shown to positively influence nursing recruitment, retention and patient outcomes.
Influence of cultivation conditions on mechanical and morphological properties of bacterial cellulose tubes

Bacterial cellulose (BC) was deposited in tubular form by fermenting Acetobacter xylinum on top of silicone tubes as an oxygenated support and by blowing different concentrations of oxygen, that is, 21% (air), 35%, 50%, and 100%. Mechanical properties such as burst pressure and tensile properties were evaluated for all tubes. The burst pressure of the tubes increased with an increase in oxygen ratio and reached a top value of 880 mmHg at 100% oxygen. The Young's modulus was approximately 5 MPa for all tubes, irrespective of the oxygen ratio. The elongation to break decreased from 30% to 10–20% when the oxygen ratio was increased. The morphology of the tubes was characterized by Scanning Electron Microscopy (SEM). All tubes had an even inner side and a more porous outer side. The cross section indicated that the tubes are composed of layers and that the amount of layers and the yield of cellulose increased with an increase in oxygen ratio. We propose that an internal vessel wall with high density is required for the tube to sustain a certain pressure. An increase in wall thickness by an increase in oxygen ratio might explain the increasing burst pressure with increasing oxygen ratio. The fermentation method used renders it possible to produce branched tubes, tubes with unlimited length and inner diameters. Endothelial cells (ECs) were grown onto the lumen of the tubes. The cells formed a confluent layer after 7 days. The tubes potential as a vascular graft is currently under investigation in a large animal model at the Centre of Vascular Engineering, Sahlgrenska University Hospital, Gothenburg. Biotechnol. Bioeng. 2007;97: 425–434. © 2006 Wiley Periodicals, Inc.
How Should Mood Disorders be Modelled?

Classification of any mental disorder is likely to have clinical utility only if it is based on a valid underlying model. The depressive disorders have long provoked debates as to whether a categorical or a dimensional model is all explanatory. This paper will argue that no single (categorical or dimensional) model is likely to be valid, and that a mix of models is required to classify, diagnose and shape management decisions for the mood disorders. After reviewing limitations to the dimensionally based official classificatory systems (DSM-IV and ICD-10), and noting some of the consequences, a set of alternative strategies is outlined. In essence, identifying syndromal ‘fuzzy sets’ from phenotypic and aetiological clustering, a model that occurs in the rest of medicine.
A Mutational Analysis of the Baculovirus Inhibitor of Apoptosis Op-IAP*

A family of antiapoptotic regulators known as inhibitors of apoptosis (IAPs) was initially identified and functionally described in baculoviruses, and IAP homologues are now known in insects, birds, and mammals. Baculovirus andDrosophila IAPs inhibit apoptosis induced byDrosophila proapoptotic proteins Reaper, HID, and GRIM and physically interact with them through their baculovirus IAP repeat (BIR) region. Here we examined the functional importance of BIR and RING finger motifs of Orgyia pseudotsugata nuclear polyhedrosis virus Op-IAP and D-IAP1 in binding to and inhibiting HID. In the absence of both the BIR1 and RING motifs, the BIR2 regions of Op-IAP and D-IAP1 were able to associate with HID and block HID-induced apoptosis. Mutation of conserved amino acid residues within the BIR and RING finger motifs revealed that the conserved residues within BIR2 were essential for Op-IAP to inhibit apoptosis. However, most of the conserved residues of the BIR2 were not required for HID binding. A region at the carboxy-proximal end of BIR2 was essential for the association of Op-IAP with HID. Thus binding to HID is necessary but not sufficient to block HID-induced apoptosis: the conserved residues within BIR2 must have an additional role in blocking apoptosis. These findings demonstrate that the region encompassing a single BIR of Op-IAP and D-IAP1 can be sufficient for physical interaction with and inhibition of apoptosis induced by HID.
Intravascular ultrasound measures of coronary atherosclerosis are associated with the Framingham risk score: an analysis from a global IVUS registry.

AIMS In addition to an adjunctive imaging platform during coronary angiography, intravascular ultrasound (IVUS) with Virtual Histology (VH) is increasingly being used to quantify coronary atherosclerosis. The relationship between VH-IVUS measures of coronary atherosclerosis and traditional cardiovascular risk factors has not been completely described. The objective of this study was to determine if an association exists between VH-IVUS measures of coronary atherosclerosis and the Framingham risk score in a prospective, multinational registry. METHODS AND RESULTS Patients enrolled from 2004-2006 at 37 multinational centres in the prospective VHIVUS Global Registry were analysed. All subjects underwent diagnostic coronary angiography followed by IVUS. A Framingham risk score (FRS) was calculated for each subject, then stratified into three exclusive estimates (<10%, 10-19%, or >or= 20%) for future coronary heart disease (CHD) event risk over 10 years. Among 531 patients, plaque volume of the most diseased 10 mm segment increased with increasing FRS (P=0.006, adjusted for multiple comparisons). Patients with higher FRS estimates of CHD risk had a higher proportion of plaque classified as thin cap fibroatheroma compared with patients in the middle and lower risk score categories (21.4% vs 15.2% and 11.3%, respectively, P=0.008, adjusted for multiple comparisons). CONCLUSIONS Using data from a large, multinational VH-IVUS registry we describe an association between the Framingham risk score and VH-IVUS measures of atherosclerosis within the most diseased 10 mm segment, namely plaque volume and the proportion of thin cap fibroatheroma.
Subpixel colocalization reveals amyloid precursor protein-dependent kinesin-1 and dynein association with axonal vesicles

Intracellular transport of vesicles and organelles along microtubules is powered by kinesin and cytoplasmic dynein molecular motors. Both motors can attach to the same cargo and thus must be coordinated to ensure proper distribution of intracellular materials. Although a number of hypotheses have been proposed to explain how these motors are coordinated, considerable uncertainty remains, in part because of the absence of methods for assessing motor subunit composition on individual vesicular cargos. We developed a robust quantitative immunofluorescence method based on subpixel colocalization to elucidate relative kinesin-1 and cytoplasmic dynein motor subunit composition of individual, endogenous amyloid precursor protein (APP) vesicles in mouse hippocampal cells. The resulting method and data allow us to test a key in vivo prediction of the hypothesis that APP can recruit kinesin-1 to APP vesicles in neuronal axons. We found that APP levels are well-correlated with the amount of the light chain of kinesin-1 (KLC1) and the heavy chain of cytoplasmic dynein (DHC1) on vesicles. In addition, genetic reduction of APP diminishes KLC1 and DHC1 levels on APP cargos. Finally, our data reveal that reduction of KLC1 leads to decreased levels of DHC1 on APP vesicles, suggesting that KLC1 is necessary for the association of DHC1 to these cargos, and help to explain previously reported retrograde transport defects generated when kinesin-1 is reduced.
Registering wishes about organ and tissue donation: personal discussion during licence renewal may be superior to online registration

Consent to organ and tissue donation is higher when the deceased has indicated a wish to donate. The Australian Organ Donor Register (AODR) is the national register of preferences regarding donation. The AODR has a number of limitations; it has no mechanism for requiring individuals to register their wishes, while the online format both raises concerns about the validity of the consent obtained and precludes personal discussion of fears and concerns about donation. A solution to these limitations is to utilize state‐based agencies that administer driving licences. This strategy ties the donation decision to an existing task (renewal of driving licences), and provides an opportunity for a personalized intervention at the time the decision is being made.
Basolateral amygdala glutamatergic activation enhances taste aversion through NMDA receptor activation in the insular cortex

In conditioned taste aversion (CTA), a subject learns to associate a novel taste with visceral malaise. Brainstem, limbic and neocortical structures have been implicated in CTA memory formation. Nevertheless, the role of interactions between forebrain structures during these processes is still unknown. The present experiment was aimed at investigating the possible interaction between the basolateral nucleus of the amygdala (BLA) and the insular cortex (IC) during CTA memory formation. Injection of a low dose of lithium chloride (30 mg/kg, i.p.) 30 min after novel taste consumption (saccharin 0.1%) induces a weak CTA. Unilateral BLA injection of glutamate (2 µg in 0.5 µL) just before low lithium induces a stronger CTA. Unilateral injection of an N‐methyl‐d‐aspartate (NMDA) receptor antagonist (AP5, 5 µg in 0.5 µL) in IC has no effect. However, AP5 treatment in IC at the same time or 1 h after the ipsilateral BLA injection reverses the glutamate‐induced CTA enhancement. Injection of AP5 in IC 3 h after BLA injection does not interfere with the glutamate effect. Moreover, the CTA‐enhancing effect of glutamate was also blocked by contralateral IC injection of AP5 at the same time. These results provide strong evidence that NMDA receptor activation in the IC is essential to enable CTA enhancement induced by glutamate infusion in the BLA during a limited time period that extends to 1 but not to 3 hours. These findings indicate that BLA–IC interactions regulate the strength of CTA. The bilateral nature of these amygdalo–cortical interactions is discussed.
Unconcious or unwanted? High school curriculum priorities: Leadership perspectives

While there has been much attention focused on the “effectiveness” of high schools meeting NCLB standards and their ability to produce students who are “college and career ready” as measured by ACT test scores, less attention has been given to how the curriculum teaches students to think critically about the world around them. Critical consciousness is the thinking necessary to participate in a democratic society and provide our students with knowledge to construct personal meaning and purpose in the world (Freire, 1988; Grundy, 1987; Kincheloe, 2008). District curriculum leadership perspectives about curriculum priorities in light of current accountability measures are important to study as curriculum leaders are charged with the responsibility of leading and facilitating educational outcomes for schools and districts. This phenomenological study described how district curriculum leaders conceptualize their roles with respect to how curricular priorities are determined in an era of accountability, and explored to what extent they believe those priorities support the development of critical consciousness for high school students. District high school curriculum leaders’ perspectives were gathered through an online survey, individual interviews, and a focus group. Their answers were analyzed utilizing a theoretical framework informed by scholarly literature related to the importance of critical consciousness in creating cultures of democratic community as a focus for education, rather than that of accountability for school improvement. Transformative leadership was found to assist with fostering a culture of democratic community. This study established that the current curricular priorities for high schools are focusing on creating more “college and career” ready students as determined by curriculum and assessment alignment to Common Core standards, higher enrollments in honors and Advanced Placement (AP) courses, and increased enrollments in post-secondary institutions, but lack the dimension of critical consciousness as a curriculum outcome to ensure cultures of democratic community. While thinking skills or
Indispensable, Expendable, or Irrelevant? Effects of Job Insecurity on the Employee Reactions to Perceived Outsourcing in the Hotel Industry

This study examines the role of job insecurity as a moderator that may trigger destructive responses by employees to perceived outsourcing of labor services. Although some studies have suggested that outsourcing might not be viewed favorably by the hotel staff, the article first argues that because outsourcing of labor can be a useful strategy for the effective functioning of a hotel, mere perceptions of outsourcing by internal employees should lead them to react favorably to the hotel in the form of citizenship (organizational citizenship behavior–organization [OCB-O]) and decreased deviance (deviant workplace behavior–organization [DWB-O]). We invoke unitarism theory, which emphasizes the shared interests of all the members of an organization. The article then argues that these reactions to outsourcing may become negative when internal employees note the presence of job insecurity, triggering decreased OCB-O and DWB-O. Data were collected from 215 in-house employees working concurrently with outsourced employees at 14 hotels in Gran Canaria (Spain). Structural equation modeling (SEM) results suggest that, contrary to expectations, perceived outsourcing leads employees to significantly increase their DWB-O, but not vary their OCB-O. Unlike OCB-O, these DWB-O reactions to perceived outsourcing became stronger among employees who were high rather than low in job insecurity. The findings suggest that job insecurity plays an expendable, but relevant, role in reactions to outsourcing that harm their success.