Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparative biokinetics and metabolism of pure monomeric, dimeric, and polymeric flavan-3-ols: a randomized cross-over study in humans.

SCOPE Flavan-3-ols are abundant polyphenols in human nutrition and are associated with beneficial health effects. The aim of this study was to comparatively investigate the metabolic fate of (-)-epicatechin, procyanidin B1, and polymeric procyanidins in a randomized cross-over study in humans. METHODS AND RESULTS Parent compounds, conjugates, and microbial metabolites were determined in plasma, urine, and faeces by HPLC-MS and GC-MS/MS. Glucuronidated, sulfated, and methylated (-)-epicatechin and 5-(3',4'-dihydroxyphenyl)-valerolactone were the dominant metabolites in blood and urine. In addition, minor amounts of procyanidin B1 and 4-hydroxy-5-(3',4'-dihydroxyphenyl)valeric acid and their conjugated metabolites were detected. The formation of 5-(3',4'-dihydroxyphenyl)-valerolactone and 4-hydroxy-5-(3',4'-dihydroxyphenyl)valeric acid varied largely between individuals as well as with the degree of polymerization of flavan-3-ols. Monomer units were not detectable in plasma or urine after procyanidin B1 and polymeric procyanidin intake. No correlation was found between the intake of flavan-3-ols and the occurrence of phenolic acids in blood and urine or the phenolic compound profiles in faeces. CONCLUSION In addition to conjugated metabolites derived from the absorption of monomeric flavan-3-ols, 5-(3',4'-dihydroxyphenyl)-valerolactone represents an important in vivo metabolite of (-)-epicatechin and procyanidin B1 produced by the gut microbiota.
Clinical performance of access flap surgery in the treatment of the intrabony defect. A systematic review and meta-analysis of randomized clinical trials.

AIM To systematically review the literature and to determine the clinical performance of conservative surgery (CS) for the treatment of intrabony defects (ID). METHODS RCTs on ID treatment with 12 months of follow-up were identified through electronic databases and hand-searched journals. Primary outcomes were tooth survival, clinical attachment (CAL) gain, probing depth (PD) reduction and gingival recession increase (REC). Weighted means and forest plots were calculated for each outcome variable 12 months after surgery. Long-term stability was explored with RCTs of at least 24 months of follow-up. Subgroup analysis was performed according to the type of flap. RESULTS Twenty-seven trials reporting 647 subjects and 734 defects were identified. Twelve months after CS, tooth survival was 98% (IQ: 96.77-100), CAL gain 1.65 mm (95% CI: 1.37-1.94; p < 0.0001), PD reduction 2.80 mm (CI: 2.43-3.18; p < 0.0001) and REC increase 1.26 mm (CI: 0.94-1.49; p < 0.0001). Longer follow-up showed similar findings. CI of CAL gain were 1.44-3.52 for recently introduced papilla preservation flaps and 1.25-1.89 mm for access flaps. CONCLUSIONS The treatment of intrabony defect with CS is associated with high tooth retention and improvement of periodontal clinical parameters. Clinical performance may vary according to the type of surgical flap used.
An Empirical Analyses of Unemployment Hysteresis and Natural Rate of Unemployment Approaches for MENA Countries

This paper aims to test natural rate of unemployment and approach of unemployment hysteresis in MENA*** countries for the period of 1991-2014 by using panel data analysis methods which considering the crosssectional dependency properties. Cross-sectional dependency test results indicate that there is cross-section dependency among all variables. According to CADF test results, unemployment rates in all countries have unit root. According to the CIPS test results, unemployment rates in MENA countries has unit root. In this context, all the impact of a shock can be seen on the unemployment rate in the mentioned region is permanent. Therefore; it can be said that the current approach of unemployment hysteresis in the region countries are valid.
Influence of biofuel crops on mosquito production and oviposition site selection

The proposed expansion of biofuels production may cause unintended land‐use changes and potentially alter ecosystem services. This study evaluated the impact of first‐generation (corn) and second‐generation (switchgrass and Miscanthus) biofuel crops on production and oviposition site selection by two vector mosquitoes, the yellow fever mosquito Aedes aegypti and the Asian tiger mosquito Aedes albopictus. Larvae of the two species were reared at varying conspecific and heterospecific densities in senescent leaf infusions prepared from one of the three biofuel crops and their survival and development time to adulthood determined. The effects of the three leaf infusions on water chemistry and oviposition site selection by the two mosquito species were also determined. Ae. albopictus females deposited significantly fewer eggs in Miscanthus than in corn infusion while Ae. aegypti females deposited significantly fewer eggs in Miscanthus than in both corn and switchgrass infusion. Survival to adulthood for both mosquito species was significantly lower in corn than in switchgrass and Miscanthus infusions; was consistently lower at high‐ (0:40 and 20:20) than at low density treatments in both switchgrass and Miscanthus infusions; and significantly lower at high intraspecific density (40:0 and 0: 40) than at high interspecific density (20:20) in Miscanthus infusion. Development time to adulthood was positively related to larval density, but was not influenced by biofuel leaf treatment. Corn infusion had lower pH values and higher salinity, conductivity, total dissolved solids (TDS), and temperature values than switchgrass and Miscanthus infusions. These findings demonstrate the potential for biofuel crops to modify the chemistry of aquatic habitats in ways that may influence mosquito production and thereby the risk of exposure to mosquito‐borne diseases.
Charting the excitability of premotor to motor connections while withholding or initiating a selected movement

In 19 healthy volunteers, we used transcranial magnetic stimulation (TMS) to probe the excitability in pathways linking the left dorsal premotor cortex and right primary motor cortex and those linking the left and right motor cortex during the response delay and the reaction time period while subjects performed a delayed response [symbol 1 (S1) ‐ symbol 2 (S2)] Go–NoGo reaction time task with visual cues. Conditioning TMS pulses were applied to the left premotor or left motor cortex 8 ms before a test pulse was given to the right motor cortex at 300 or 1800 ms after S1 or 150 ms after S2. S1 coded for right‐hand or left‐hand movement, and S2 for release or stopping the prepared movement. Conditioning of the left premotor cortex led to interhemispheric inhibition at 300 ms post‐S1, interhemispheric facilitation at 150 ms post‐S2, and shorter reaction times in the move‐left condition. Conditioning of the left motor cortex led to inhibition at 1800 ms post‐S1 and 150 ms post‐S2, and slower reaction times for move‐right conditions, and inhibition at 300 and 1800 ms post‐S1 for move‐left conditions. Relative motor evoked potential amplitudes following premotor conditioning at 150 ms post‐S2 were significantly smaller in ‘NoGo’ than in ‘Go’ trials for move‐left instructions. We conclude that the excitability in left premotor/motor right motor pathways is context‐dependent and affects motor behaviour. Thus, the left premotor cortex is engaged not only in action selection but also in withholding and releasing a preselected movement generated by the right motor cortex.
CML42-Mediated Calcium Signaling Coordinates Responses to Spodoptera Herbivory and Abiotic Stresses in Arabidopsis1[W][OA]

In the interaction between Arabidopsis (Arabidopsis thaliana) and the generalist herbivorous insect Spodoptera littoralis, little is known about early events in defense signaling and their link to downstream phytohormone pathways. S. littoralis oral secretions induced both Ca2+ and phytohormone elevation in Arabidopsis. Plant gene expression induced by oral secretions revealed up-regulation of a gene encoding a calmodulin-like protein, CML42. Functional analysis of cml42 plants revealed more resistance to herbivory than in the wild type, because caterpillars gain less weight on the mutant, indicating that CML42 negatively regulates plant defense; cml42 also showed increased aliphatic glucosinolate content and hyperactivated transcript accumulation of the jasmonic acid (JA)-responsive genes VSP2 and Thi2.1 upon herbivory, which might contribute to increased resistance. CML42 up-regulation is negatively regulated by the jasmonate receptor Coronatine Insensitive1 (COI1), as loss of functional COI1 resulted in prolonged CML42 activation. CML42 thus acts as a negative regulator of plant defense by decreasing COI1-mediated JA sensitivity and the expression of JA-responsive genes and is independent of herbivory-induced JA biosynthesis. JA-induced Ca2+ elevation and root growth inhibition were more sensitive in cml42, also indicating higher JA perception. Our results indicate that CML42 acts as a crucial signaling component connecting Ca2+ and JA signaling. CML42 is localized to cytosol and nucleus. CML42 is also involved in abiotic stress responses, as kaempferol glycosides were down-regulated in cml42, and impaired in ultraviolet B resistance. Under drought stress, the level of abscisic acid accumulation was higher in cml42 plants. Thus, CML42 might serve as a Ca2+ sensor having multiple functions in insect herbivory defense and abiotic stress responses.
On the Performance Limits of Cryogenically Operated SiGe HBTs and Its Relation to Scaling for Terahertz Speeds

The goal of achieving terahertz (THz) transistors within the silicon material system has generated significant recent interest. In this paper, we use operating temperature as an effective way of gaining a better understanding of the performance limits of SiGe HBTs and their ultimate capabilities for achieving THz speeds. Different approaches for vertical profile scaling and reduction of parasitics are addressed, and three prototype fourth-generation SiGe HBTs are compared and evaluated down to deep cryogenic temperatures, using both dc and ac measurements. A record peak fT/fmax of 463/618 GHz was achieved at 4.5 K using 130-nm lithography (309/343 GHz at 300 K), demonstrating the feasibility of reaching half-THz fT and fmax simultaneously in a silicon-based transistor. The BVCEO of this cooled SiGe HBT was 1.6 V at 4.5 K (BVCBO = 5.6 V), yielding a record fT times BVCEO product of 750 GHzldrV (510 GHzldrV at 300 K). These remarkable levels of transistor performance and the associated interesting device physics observed at cryogenic temperatures in these devices provide important insights into further device scaling for THz speeds at room temperature. It is predicted in a new scaling roadmap that fT/fmax of room-temperature SiGe HBTs could potentially achieve 782/910 GHz at a BVCEO of 1.1 V at the 32-nm lithographic node.
Gender differences in glutathione metabolism in Alzheimer's disease

The mechanism underlying Alzheimer's disease (AD), an age‐related neurodegenerative disease, is still an area of significant controversy. Oxidative damage of macromolecules has been suggested to play an important role in the development of AD; however, the underlying mechanism is still unclear. In this study, we showed that the concentration of glutathione (GSH), the most abundant intracellular free thiol and an important antioxidant, was decreased in red blood cells from male AD patients compared with age‐ and gender‐matched controls. However, there was no difference in blood GSH concentration between the female patients and female controls. The decrease in GSH content in red blood cells from male AD patients was associated with reduced activities of glutamate cysteine ligase and glutathione synthase, the two enzymes involved in de novo GSH synthesis, with no change in the amount of oxidized glutathione or the activity of glutathione reductase, suggesting that a decreased de novo GSH synthetic capacity is responsible for the decline in GSH content in AD. These results showed for the first time that GSH metabolism was regulated differently in male and female AD patients. © 2005 Wiley‐Liss, Inc.
Investigation of serotype distribution and resistance genes profile in group B Streptococcus isolated from pregnant women: a Chinese multicenter cohort study

We surveyed the group B Streptococcus (GBS) strains isolated from four teaching hospitals during 1‐year period to investigate the current serotypes and antimicrobial resistance status of these strains. A total of 231 non‐duplicate colonizing GBS isolates were collected from pregnant women. Antimicrobial susceptibility of these isolates was tested by the disk diffusion method. Serotype was performed by a multiplex polymerase chain reaction (PCR) method. Analysis of the resistance mechanisms was performed by PCR amplification and DNA sequencing. Seven serotypes (Ia, Ib, II, III, V, VI, and VIII) were identified, and the prevalence ranged from 0.9 to 35.9%. All isolates were susceptible to the penicillin, ceftriaxone, and vancomycin. The resistance of all the isolates to erythromycin, clindamycin, and levofloxacin was 61.5, 51.9, and 35.5%, respectively. The erythromycin resistance was mainly associated with the genes ermB and ermB‐mef(A/E) (69.8%). The most predominant phenotype was cMLSB (77.5%). Five gene panels, including gyrA, parC, parE, gyrA‐parC, and gyrA‐parC‐parE, were detected. The most predominant genotype was gyrA‐parC‐parE triple mutation (69.5%). The S81L in gyrA gene, S79Y mutation in parC gene, and H225Y mutation in parE gene were discovered. The isolates with serotype III, V, and Ia were the most important clone concerning the prevalence and resistance.
Evaluation of (111) Indium-Labeled Autologous Leukocyte Scintigraphy for the Diagnosis of Chronic Osteomyelitis in Patients With Grade IV Pressure Ulcers, as Compared With a Standard Diagnostic Protocol

Diagnosing osteomyelitis in patients with pressure ulcers is complicated by overlying soft-tissue inflammation and reactive bone formation. We set out to evaluate the efficacy of indium scanning in the diagnosis of chronic osteomyelitis in spinal-cord-injury patients with grade IV pressure ulcers. The goal was to estimate the sensitivity and specificity of indium scanning as compared with diagnostic modalities previously evaluated by the principal investigator. Our method was a retrospective chart review of cases at a university hospital. Eleven patients with pressure sores and suspected chronic osteomyelitis underwent indium scanning. The results were compared with ostectomy specimens taken at the time of reconstructive surgery and/or bone biopsy. The sensitivity and specificity were 100% and 50%, respectively. Indium scanning appears to be more sensitive than specific. These data suggest that the value of indium scanning may primarily be to rule out osteomyelitis and not as a primary diagnostic modality.
Disparities in Cardiovascular Research Output and Citations From 52 African Countries: A Time‐Trend, Bibliometric Analysis (1999–2008)

Background Cardiovascular research output and citations of publications from Africa have historically been low yet may be increasing. However, data from the continent are limited. Methods and Results To evaluate the cardiovascular research output and citations from 52 African countries between 1999 and 2008, we created a bibliometric filter to capture cardiovascular research articles published in the Web of Knowledge based on specialist journals and title words. Two coauthors with expertise in cardiovascular medicine tested and refined this filter to achieve >90% precision and recall. We matched retrieved records with their associated citation reports and calculated the running 5‐year citation count postpublication, including the year of publication. Publications from Africa were identified by author addresses. South Africa published 872 cardiovascular research papers, Egypt 393, Tunisia 264, and Nigeria 192 between 1999 and 2008. The number of publications increased over the time period for a small number of countries (range 0.1 to 4.8 more publications per year by fractional count). Most countries' citations were low (<50), but citations were greatest for South Africa (7063), Egypt (2557), Tunisia (903), and Nigeria (540). The same countries had the greatest annual increase in 5‐year citation index values: 65 (95% CI: 30, 99) for South Africa, 46 (34, 58) for Egypt, 22 (15, 28) for Tunisia, and 8 (2, 14) for Nigeria. The burden of cardiovascular disease had a weak and inconsistent relationship to cardiovascular publications (r2=0.07, P=0.05). Greater gross domestic product was associated with more cardiovascular publications in 2008 (r2=0.53, P<0.0001). Conclusions The increases in cardiovascular research outputs from Africa are concentrated in a few countries. The reasons for regional differences in research outputs require further investigation, particularly relative to competing disease burdens. Higher prioritization of cardiovascular research funding from African countries is warranted.
China Pro-Growth Monetary Policy and Its Asymmetric Transmission

China monetary policy, as well as its transmission, is yet to be understood by researchers and policymakers. In the spirit of Taylor (1993, 2000), we develop a tractable framework that approximates practical monetary policy of China. The framework, grounded in relevant institutional elements, allows us to quantify the policy effects on output and prices. We find strong evidence that monetary policy is designed to support real GDP growth mandated by the central government while resisting inflation pressures and that contributions of monetary policy shocks to the GDP fluctuation are asymmetric across different states of the economy. These findings highlight the role of M2 growth as a primary instrument and the bank lending channel to investment as a key transmission mechanism for monetary policy. Our analysis sheds light on institutional constraints on a gradual transition from M2 growth to the nominal policy interest rate as a primary instrument for monetary policy.
Mutations in Escherichia coli dnaA which suppress a dnaX(Ts) polymerization mutation and are dominant when located in the chromosomal allele and recessive on plasmids

Extragenic suppressor mutations which had the ability to suppress a dnaX2016(Ts) DNA polymerization defect and which concomitantly caused cold sensitivity have been characterized within the dnaA initiation gene. When these alleles (designated Cs, Sx) were moved into dnaX+ strains, the new mutants became cold sensitive and phenotypically were initiation defective at 20 degrees C (J.R. Walker, J.A. Ramsey, and W.G. Haldenwang, Proc. Natl. Acad. Sci. USA 79:3340-3344, 1982). Detailed localization by marker rescue and DNA sequencing are reported here. One mutation changed codon 213 from Ala to Asp, the second changed Arg-432 to Leu, and the third changed codon 435 from Thr to Lys. It is striking that two of the three spontaneous mutations occurred in codons 432 and 435; these codons are within a very highly conserved, 12-residue region (K. Skarstad and E. Boye, Biochim. Biophys. Acta 1217:111-130, 1994; W. Messer and C. Weigel, submitted for publication) which must be critical for one of the DnaA activities. The dominance of wild-type and mutant alleles in both initiation and suppression activities was studied. First, in initiation function, the wild-type allele was dominant over the Cs, Sx alleles, and this dominance was independent of location. That is, the dnaA+ allele restored growth to dnaA (Cs, Sx) strains at 20 degrees C independently of which allele was present on the plasmid. The dnaA (Cs, Sx) alleles provided initiator function at 39 degrees C and were dominant in a dnaA(Ts) host at that temperature. On the other hand, suppression was dominant when the suppressor allele was chromosomal but recessive when it was plasmid borne. Furthermore, suppression was not observed when the suppressor allele was present on a plasmid and the chromosomal dnaA was a null allele. These data suggest that the suppressor allele must be integrated into the chromosome, perhaps at the normal dnaA location. Suppression by dnaA (Cs, Sx) did not require initiation at oriC; it was observed in strains deleted of oriC and which initiated at an integrated plasmid origin.
Arteriovenous access creation defers chronic hemodialysis initiation.

AIMS This study evaluated whether arteriovenous access (AVA) creation before hemodialysis might affect the rate of decline of renal function. METHODS This was a retrospective, case-control study comparing two groups of 80 patients each. The AVA group underwent AVA creation more than 2 months before initiation of chronic hemodialysis (CHD). The catheter group (i.e., the control group) commenced CHD through a temporary catheter. The catheter group patients were matched with AVA group patients in terms of age, gender, nature of underlying disease, and rate of decline in estimated glomerular filtration rate (eGFR) before AVA creation. The zero point (Z-point) was defined as the date of AVA creation for AVA patients, or the date on which the same eGFR was attained by each catheter group patient compared with a matched AVA patient. Time-to-dialysis was defined as the interval from the Z-point to the date of initiation of dialysis. The rates of change in eGFR before and after the Z-point were also measured. RESULTS The AVA and catheter groups were similar in terms of baseline characteristics. The Z-point eGFR (ml/min/1.73 m2) was 11.4±3.1 in the AVA group and 11.3±3.2 in the catheter group. The eGFR at the time of dialysis was 6.4±2.0 in the AVA group and 6.1±1.9 in the catheter group. The mean and median dialysis-free time was longer in the AVA than in the catheter group (14.2±9.4 vs. 5.9±4.1 months, 13.1 (3-41) vs. 5.0 (2-17) months, p<0.001). Multivariate proportional Cox's hazard modeling showed that the AVA group and the Z-point eGFR were each independent predictors of the time to initiation of CHD. The mean changes in eGFR per month (delta eGFR) before the Z-point were similar for the two groups. For the AVA group, the mean delta eGFR was lower after the Z-point compared to before (-0.63 vs. -0.21 ml/min/1.73 m2, p=0.002). For the catheter group, the mean delta eGFR was similar before and after the Z-point (-0.63 vs. -0.67 ml/min/1.73 m2). The mean delta eGFR after the Z-point was less in the AVA group compared to the catheter group (-0.67 vs. -0.21 ml/min/1.73 m2, p=0.002). CONCLUSION In this retrospective observational study, AVA creation appears to retard the rate of decline in eGFR and to defer CHD initiation.