Miracle-dz/UniFAR-FTUs huggingface dataset:
STRUCTURE OF STREPTOCOCCAL CELL WALLS. 3. CHARACTERIZATION OF AN ALANINE-CONTAINING GLUCOSAMINYLMURAMIC ACID DERIVATIVE LIBERATED BY LYSOZYME FROM STREPTOCOCCAL GLYCOPEPTIDE.

A glycopeptide (also called mucopeptide) fraction of bacterial cell walls, believed to be associated with shape and structural stability of microorganisms, may, in the case of Group A streptococci, be isolated with relative ease as the formamide-insoluble residue of protease-treated cell walls (1). Analyses and certain susceptibilities to enzymic lysis of this fraction have been reported (1, 2) as well as the recognition in the lysate of a disaccharide containing 2-deoxy-2-acetamido-n-glucose and N-acetylmuramic acid (2). The present article supplies experimental details and in several regards expands the data of our preliminary communication (2).
Communication barriers to family farm succession planning

Many farm families fail to take succession planning actions even when information is available on the tax, business organization, and investment aspects of this process. In semi-structured interviews conducted with multi-generational members of nine small farm families in Pennsylvania, most respondents attributed a high level of importance to succession planning, but conceded that they had not done enough Communication Barriers to Family Farm Succession Planning 10/26/09 08:05:37
Isolation of a novel TP53 target gene from a colon cancer cell line carrying a highly regulated wild‐type TP53 expression system

We established a colon cancer cell line SW480‐LOWTP53‐1 carrying a wild‐type TP53 transgene that is inducible under control of the lactose operon. Induction of this transgene by isopropyl‐β‐D‐thiogalactoside (IPTG) arrests growth of the transfected cells. To investigate cellular responses related to the TP53 signaling pathway to induce growth arrest, we applied a differential display method to screen mRNAs isolated from this cell line and looked for genes whose expression was activated or suppressed after induction of wild‐type TP53. Subsequent Northern blot analysis confirmed that expression of one novel gene was regulated by wild‐type TP53. The cDNA, termed TP53TG1 (TP53 target gene 1), contained an open reading frame of 270 nucleotides encoding 90 amino acids. Under conditions of cellular stress (ultraviolet irradiation or exposure to bleomycin or cisplatin), expression of TP53TG1 was induced in a wild‐type TP53‐dependent manner, indicating that this gene is likely to play an important role in the signaling pathway of TP53 and may function in response to cellular damage. Genes Chromosomes Cancer 23:1–9, 1998. © 1998 Wiley‐Liss, Inc.
Liver Receptor Homologue-1 Mediates Species- and Cell Line-specific Bile Acid-dependent Negative Feedback Regulation of the Apical Sodium-dependent Bile Acid Transporter*

Intestinal reclamation of bile salts is mediated in large part by the apical sodium-dependent bile acid transporter (ASBT). The bile acid responsiveness of ASBT is controversial. Bile acid feeding in mice results in decreased expression of ASBT protein and mRNA. Mouse but not rat ASBT promoter activity was repressed in Caco-2, but not IEC-6, cells by chenodeoxycholic acid. A potential liver receptor homologue-1 (LRH-1) cis-acting element was identified in the bile acid-responsive region of the mouse but not rat promoter. The mouse, but not rat, promoter was activated by LRH-1, and this correlated with nuclear protein binding to the mouse but not rat LRH-1 element. The short heterodimer partner diminished the activity of the mouse promoter and could partially offset its activation by LRH-1. Interconversion of the potential LRH-1 cis-elements between the mouse and rat ASBT promoters was associated with an interconversion of LRH-1 and bile acid responsiveness. LRH-1 protein was found in Caco-2 cells and mouse ileum, but not IEC-6 cells or rat ileum. Bile acid response was mediated by the farnesoid X receptor, as shown by the fact that overexpression of a dominant-negative farnesoid X-receptor eliminated the bile acid mediated down-regulation of ASBT. In addition, ASBT expression in farnesoid X receptor null mice was unresponsive to bile acid feeding. In summary cell line- and species-specific negative feedback regulation of ASBT by bile acids is mediated by farnesoid X receptor via small heterodimer partner-dependent repression of LRH-1 activation of the ASBT promoter.
STUDIES OF COPROPORPHYRIN. II. AN INVESTIGATION OF THE CONTRIBUTION TO THE URINARY COPROPORPHYRIN OF HEMOGLOBIN AND OF BACTERIAL METABOLISM IN THE INTESTINAL TRACT.

Stokvis (1) advanced the idea that blood in the intestinal tract was the source of the urinary porphyrin. Schumm (2) also held this belief. Subsequent studies, notably those of Fischer and Hilmer (3), Brugsch (4, 5), and Kaunitz (6) indicated that a variable fraction of the urinary coproporphyrin was thus derived. The presence of a porphyrin in meconium (2, 7, 8), later identified by Waldenstr6m as coproporphyrin I (9), together with a number of other evidences of an endogenous formation, which are discussed in detail in recent reviews(10, 11), have made it reasonably clear that at most not more than a certain fraction of the, urinary coproporphyrin (UCP) could be regarded as originating in the intestinal tract. The purpose of the present investigation was to obtain more definite information as to the size of this fraction, under various experimental and clinical conditions. It was also determined to study the relative percentage of coproporphyrin isomers composing any increases which might be observed, since previous studies bearing on these questions had dealt only with the total coproporphyrin. It was conceivable on a priori reasoning, that any one or a combination of the following might alter the total coproporphyrin concentration or the isomer distribution in the urine: (1) A formation of coproporphyrin III from hemoglobin, by the bacterial flora of the colon, with reabsorption and excretion in the urine. Jakob (12) showed that pure cultures of E. coli produce small amounts of coproporphyrin III in the presence of hemoglobin. Snapper (13-15)
PHYSICAL ACTIVITY AND ITS RELATIONSHIP TO RODEO INJURY AND SUCCESS

The purpose of this study was to determine the interrelationship among physical activity, injury, and success of rodeo athletes. Seventy-two male Professional Rodeo Cowboys' Association members served as participants and were grouped into timed, steer-wrestling, saddle- bronc, bareback, bull-riding, and multiple rough-stock events. Participants completed demographic and overall physical activity questionnaires. Sixty-two percent of the subjects participated in regular exercise for a minimum of 2 days per week. Pearson bivariate correlation coefficients revealed weak correlations (r = 0.151, 20.188, and 20.074; p > 0.05) among metabolic (MET) level, earnings rate, and injury rate. A 1-way between-subjects analysis of variance indicated no differences in MET level among groups. Physical activity did not affect performance or injury rate, and competitors in various events did not have different levels of energy expenditure. Therefore, rodeo sports-medicine personnel should encourage athletes to engage in other sport- specific training activities to help prevent rodeo injuries.
[A 10-day sequential therapy for eradication of Helicobacter pylori infection in children].

OBJECTIVE To evaluate the clinical effect of a 10-day sequential therapy which was made up of omeprazole, clarithromycin, amoxicillin-clavulanate and metronidazole for the eradication of Helicobacter pylori (Hp) infection in children. METHOD A total of 214 children with abdominal pain, who were confirmed to have Hp infection through endoscopy, biopsy, and Hp culture. The 214 cases were randomly divided into four groups. A 10-day sequential therapy group accepted omeprazole 0.8 - 1.0 mg/(kg·d) plus amoxicillin-clavulanate 50 mg/(kg·d) for five days and omeprazole 0.8 - 1.0 mg/(kg·d), clarithromycin 20 mg/(kg·d) and metronidazole 20 mg/(kg·d) for the remaining five days. The 7-day triple therapy group, 10-day triple therapy group and 14-day triple therapy group received omeprazole 0.8 - 1.0 mg/(kg·d), amoxicillin-clavulanate 50 mg/(kg·d) and clarithromycin 20 mg/(kg·d) for 7 days,10 days,14 days, respectively. All drugs were given twice daily. All these patients received (13)C urea breath test ((13)C-UBT) four weeks after the treatment. RESULT Finally, 199 patients were followed up, and the total rate of loss to follow-up was 7.0% (15/214). Hp eradication rate was 85.2% and 90.2% in the 10-day sequential therapy group on intention to treat (ITT) and per protocol (PP) analyses, 66.0% and 71.4% in the 7-day triple therapy group on ITT and PP analyses; 60.0% and 67.3% in 10-day triple therapy group on ITT and PP analyses, and 78.8% and 82.0% in patients who received the 10-day sequential regimen on ITT and PP analyses, respectively. By ITT analysis, there was significantly difference between the 10-day sequential therapy group and 7-day or 10-day triple therapy group (P < 0.05), while no significant difference was found between the 10-day sequential therapy group and 14-day triple therapy group (P > 0.05). The results of the ITT analysis and the PP analysis were the same. The four groups had neither significant difference in abdominal pain relief (P > 0.05) nor in incidence of adverse reactions (P > 0.05). CONCLUSION The 10-day sequential regimen was significantly more effective than both 7-day triple regimen and 10-day triple regimen, while had the same eradication rate compared with the 14-day sequential therapy. But 10-day triple regimen to eradicate Hp infection in children had the advantages such as short course of treatment and better compliance.
Origin of language and origin of languages

The question of monogenesis vs. polygenesis of human languages was essentially neglected by contemporary linguistics until the appearance of the research on the genetics of human populations by L. L. Cavalli-Sforza and his collaborators, which brought to light very exciting parallels between the distribution of human populations and that of language families. The present paper highlights some aspects of the history of the problem and some points of the contemporary discussion. We first outline the “Biblical paradigm”, which persisted until the 18th century even in scientific milieus. Then, we outline some aspects of the 19th century debate about monogenesis vs. polygenesis of languages and about the relationships between languages and human populations: in particular, we will discuss the views of Darwin on the one hand and of some linguists on the other (Schleicher, M. Müller, Whitney and Trombetti). It will be seen that their positions only partly coincide; at any rate, it will be shown that Darwin was partly inspired by the problems of the genealogy of languages and that the linguists, for their part, took account of Darwin’s views. Turning to today’s debate, we first present the positions of the linguists arguing for monogenesis, namely J. Greenberg and M. Ruhlen, as well as the criticisms raised against their methods by the majority of linguists. Other scholars, such as D. Bickerton or N. Chomsky, essentially argue, from different points of view, that the problem of monogenesis vs. polygenesis of languages is a “pseudo-problem”. We however think that, although the question cannot be reasonably solved by linguistic means, it cannot be discarded as meaningless: it is an anthropological rather than a linguistic problem. We present some reflections and suggestions, in the light of which the monogenetic hypothesis appears as more tenable than the polygenetic one.
GeneChip® analysis shows altered mRNA expression of transcripts of neurotransmitter and signal transduction pathways in the cerebral cortex of portacaval shunted rats

Identifying the gene expression changes induced by hepatic encephalopathy (HE) leads to a better understanding of the molecular mechanisms of HE‐induced neurological dysfunction. Using GeneChip® and real‐time PCR, the present study evaluated the gene expression profile of rat cerebral cortex at 4 weeks after portacaval shunting. Among 1,263 transcripts represented on the chip, mRNA levels of 31 transcripts were altered (greater than twofold; 16 increased and 15 decreased) in the portacaval shunted (PCS) rat compared to sham control. Changes observed by GeneChip® analysis were confirmed for 20 transcripts (8 increased, 7 decreased, and 5 unchanged in PCS rat brain) by real‐time PCR. Neurotransmitter receptors, transporters, and members of the second messenger signal transduction are the major groups of genes altered in PCS rat brain. Of importance was that the increased heme oxygenase‐1 and decreased Cu,Zn‐superoxide dismutase expression observed raise the possibility of oxidative stress playing a pathogenic role in chronic HE. © 2002 Wiley‐Liss, Inc.
Ventricular arrhythmias during race recovery in Standardbred Racehorses and associations with autonomic activity.

BACKGROUND A lack of information on normal heart rhythm at maximal effort hampers investigation of poor performance and sudden death in Standardbred racing. HYPOTHESIS/OBJECTIVE To characterize rhythm variations during scheduled racing in clinically normal Standardbred horses. ANIMALS Two hundred and eighty-eight Standardbred horses competing in 40 scheduled races at a Southern Ontario racetrack. METHODS Observational study, convenience sampling: Heart rhythm was monitored by ECG from harnessing to postrace recovery and assessed visually and by examining heart rate intervals. Rhythm variations were used as response variables in multivariate analysis of race records detailing signalment, race, and race outcome. RESULTS Monitoring involved 345 individual horse or race events. Occasional, isolated premature cycles, only, occurred during the race. Postrace, sudden cardiac slowing (punctuated deceleration [PD]) appeared in 42 events (12.2%). Only premature ventricular complexes were exhibited in 40 events postrace (11.6%), whereas 55 (15.9%) exhibited complex ventricular arrhythmias (CVA) including torsades-like polymorphic ventricular tachycardia, 34.5% of these being closely associated with PD (odds ratio = 8). Predispositions to CVA were found for horses parked at the 1/2 mile (odds ratio = 3), and trotters breaking in the stretch (odds ratio = 38). Horses spontaneously reverted to sinus rhythm and no sudden death events were encountered. CONCLUSIONS AND CLINICAL IMPORTANCE Arrhythmias occur frequently in racing Standardbreds during cardiac deceleration, often associated with sudden, rapid increases in vagal tone. Circumstances imposing unusual demand and racing at the trot appear to predispose. Findings provide insight into possible mechanisms of sudden death.
A Large Variation in the Rates of Synonymous Substitution for RNA Viruses and Its Relationship to a Diversity of Viral Infection and Transmission Modes

Abstract RNA viruses successfully adapt to various environments by repeatedly producing new mutants, often through generating a number of nucleotide substitutions. To estimate the degree of variation in mutation rates of RNA viruses and to understand the source of such variation, we studied the synonymous substitution rate because synonymous substitution is exempt from functional constraints at the protein level, and its rate reflects the mutation rate to a great extent. We estimated the synonymous substitution rates for a total of 49 different species of RNA viruses, and we found that the rates had tremendous variation by 5 orders of magnitude (from 1.3 × 10−7 to 6.2 × 10−2 /synonymous site/year). Comparing the synonymous substitution rates with the replication frequencies and replication error rates for the RNA viruses, we found that the main source of the rate variation was differences in the replication frequency because the rates of replication error were roughly constant over different RNA viruses. Moreover, we examined a relationship between viral life strategies and synonymous substitution rates to understand which viral life strategies affect replication frequencies. The results show that the variation of synonymous substitution rates has been influenced most by either the difference in the infection modes or the differences in the transmission modes. In conclusion, the variation of mutation rates for RNA viruses is caused by different replication frequencies, which are affected strongly by the infection and transmission modes.
From quality gurus and TQM to ISO 9001:2015: a review of several quality paths

A revision of several paths for the Quality journey is presented: from Quality Gurus and Total Quality Management (TQM) models to the ISO 9000 International Standards Series. Since ISO 9001:2008 is now in the revision process to the expected ISO 9001:2015 version, an analysis is made of he proposed changes and the underlying reasons and the impacts foreseen on the more than 1.3 Million certified organizations. This revision should be a step towards TQM and reflect the changes of an increasingly complex, demanding and dynamic environment, while assuring that complying organizations are able to provide conformity products and services that satisfy their customers. Major benefits are expected such as less emphasis on documentation and new/reinforced approaches: consideration of Organizational Context and (relevant) Stakeholders, Risk Based thinking and Knowledge Management. Comments and recommendations are presented for organizations wishing to implement or update their Quality Systems accordingly to ISO 9001:2015.
Sequential therapy and prognostic factors in metastatic renal cell carcinoma: single centre experience.

PURPOSE In advanced stage renal cell cancer (RCC), overall survival (OS) of patients has been prolonged due to targeted therapies. To date, there are several prognostic risk models that have been developed for metastatic RCC (mRCC). The purpose of this study was to evaluate the outcomes of the sequential therapy (IFN-α, tyrosine kinase inhibitors/TKIs, m-TOR inhibitor) and prognostic factors in patients with mRCC, especially those with bone metastasis. METHODS We retrospectively examined the data of 82 patients with pathologically proven mRCC who were followed-up and treated at the Medical Oncology Clinic of the Dr A.Y Oncology Hospital between 2005 and 2013. RESULTS Median OS was 23 months in all patients with mRCC and 20 months in patients treated with TKIs. According to MSKCC and HENG risk classifications, median OS differed between the groups (p=0.02, p<0.001, respectively). Median OS was lower in patients with isolated bone metastasis compared to those with lung metastasis (16 vs 24 months, p=0.25). Median OS improved with increasing number of sequential therapies (p=0.08). CONCLUSION This study confirmed the correlation between MSKCC and HENG risk models and survival data. Additionally, it was shown that increase of the number of therapeutic lines in sequential therapy prolonged survival and that bone metastases were negative prognostic factors.
Genotoxic-activated G 2M checkpoint exit is dependent on CDC 25 B phosphatase expression

Cell cycle arrest at the G2-M checkpoint is an essential feature of the mechanisms that preserve genomic integrity. CDC25 phosphatases control cell cycle progression by dephosphorylating and activating cyclin-dependent kinase/cyclin complexes. Their activities are, therefore, tightly regulated to modulate cell cycle arrest in response to DNA damage exposure. Here, we report that overexpression of CDC25B affects viability, reduces clonogenic efficiency, and increases sensitivity of cancer cells to a genotoxic agent. We show that ectopic expression of CDC25B results in bypass of a genotoxic-induced G2-M checkpoint. In addition, cancer cells constitutively expressing high level of CDC25B are shown to be prone to exit prematurely from the G2-M checkpoint arrest and to enter mitosis. Finally, we show that this exit is dependent on CDC25B expression. Together with previous results, our data strongly support a model in which CDC25B is the key phosphatase that controls entry into mitosis after DNA damage, thus emphasizing the relevance of its overexpression in many human tumors. [Mol Cancer Ther 2006;5(6):1446–51]
A unified analysis of executive pay: the case of the banking industry

This study examines executive compensation determinants in the U.S. banking industry. Multiple theories of executive pay are discussed and tested using a relatively homogenous sample. We perform an in-depth look at the corporate governance and ownership structure of the companies selected. We explore the simultaneous relationship between compensation, firm performance, and board strength, exploiting variables unique to the banking industry. Our primary finding is that after controlling for both regulatory oversight and external market discipline, a strong board is associated with higher firm performance and lower levels of executive pay, consistent with such a board of directors providing a strong monitoring function.
Dentoskeletal changes in Class II malocclusion patients after treatment with the Twin Block functional appliance

OBJECTIVES: This prospective clinical study evaluated dentoskeletal changes in Class II malocclusion patients after treatment with the Twin Block functional appliance. METHODS: The treatment group consisted of 19 subjects (mean age 9.5 years) and the control group consisted of 19 subjects (mean age 9.9 years) situated before the pubertal growth spurt. Unpaired Student's t test showed the sample homogeneity at the beginning of the study. Initial (T1) and one year follow-up (T2) cephalometric radiographs were obtained for all subjects. Wilcoxon test and Mann-Whitney test were used to evaluate changes intra and inter groups from T1 to T2. RESULTS: A Class I molar relationship was achieved in 15 subjects of the TB group while no modification occurred in the control group. No significant effect was observed either in the maxilla or in the vertical pattern. A significant increase in total mandibular length and an anterior displacement of the mandibular position occurred in the treated group (p<0.05) as well as an overjet reduction, influenced by significant upper incisor retroclination and lower incisor proclination (p<0.05). CONCLUSION: Class II treatment with the Twin Block appliance in Brazilian patients showed skeletal and dental effects, including increase in mandibular length and incisors compensation, respectively.
Systematic functional characterization of putative zinc transport genes and identification of zinc toxicosis phenotypes in Drosophila melanogaster

SUMMARY The heavy metal zinc is an essential component of the human diet and is incorporated as a structural component in up to 10% of all mammalian proteins. The physiological importance of zinc homeostasis at the cellular level and the molecular mechanisms involved in this process have become topics of increasing interest in recent years. We have performed a systematic functional characterization of the majority of the predicted Drosophila Zip (zinc/iron regulated transporter-related protein) and ZnT genes, using the Gal4-UAS system to carry out both ubiquitous and targeted over-expression and suppression studies for 13 of the 17 putative zinc transport genes identified to date. We found that six of these 13 genes may be essential for fly viability and that three of the remaining seven demonstrate over-expression phenotypes. Our findings reaffirm the previously proposed function of dZnT63C (CG17723: FBgn005432) as an important zinc efflux protein and indicate that the fly homolog of hZip1, dZip42C.1 (CG9428: FBgn0033096), is a strong zinc importer in Drosophila. By combining over-expression of dZip42C.1 with suppression of dZnT63C we were able to produce easily identifiable zinc toxicosis phenotypes, which can be rescued or worsened by modifying dietary zinc content. Our findings show that a genetically based zinc toxicosis situation can be therapeutically treated or exacerbated by modifications to the diet, providing a sensitized background for future, more detailed studies of Zip/ZnT function.