Miracle-dz/UniFAR-FTUs huggingface dataset:
Progression of autoimmune hepatitis is mediated by IL‐18‐producing dendritic cells and hepatic CXCL9 expression in mice

Clinical manifestations of autoimmune hepatitis (AIH) range from mild chronic to acute, sometimes fulminant hepatitis. However, it is unknown how the progression to fatal hepatitis occurs. We developed a mouse model of fatal AIH by inducing a concurrent loss of forkhead box P3+ regulatory T cells and programmed cell death‐1 (PD‐1)‐mediated signaling. In this model, dysregulated follicular helper T cells in the spleen are responsible for the induction, and the C‐C chemokine receptor 6/C‐C chemokine ligand 20 axis is crucial for the migration of these T cells into the liver. Using this fatal AIH model, we aimed to clarify key molecules triggering fatal AIH progression. During progression, T‐bet together with interferon (IFN)‐γ and C‐X‐C chemokine receptor (CXCR)3 were highly expressed in the inflamed liver, suggesting helper T (Th)1‐type inflammation. T cells that dominantly expanded in the spleen and the inflamed liver were CXCR3‐expressing CD8+ T cells; depletion of these CD8+ T cells suppressed AIH progression. Expression of one CXCR3 ligand, chemokine (C‐X‐C motif) ligand (CXCL)9, was elevated in the liver. CXCL9‐expressing macrophages/Kupffer cells were colocalized with infiltrating T cells, and in vivo administration of anti‐CXCL9 suppressed AIH progression. In addition, serum levels of interleukin (IL)‐18, but not IL‐1β, were elevated during progression, and dendritic cells in the spleen and liver highly produced IL‐18. In vivo administration of anti‐IL‐18R suppressed the increase of splenic CXCR3+ T cells and the progression to fatal AIH. Moreover, tumor necrosis factor alpha, but not IFN‐γ, was involved in up‐regulating CXCL9 in the liver and for increased serum levels of IL‐18. Conclusion: These data suggest that, in our mouse model, fatal progression of AIH is mediated by IL‐18‐dependent differentiation of T cells into Th1 cells and effector T cells, respectively, and that CXCR3‐CXCL9 axis‐dependent migration of those T cells is crucial for fatal progression. (Hepatology 2014;60:224–236)
Historical deforestation due to expansion of crop demand: implications for biofuels

The report presents an independent estimate of the part of LUC emissions due to deforestation, starting from the 29% of historical deforestation area (and estimated emissions) caused by expansion of different crops. The deforestation area and emissions per tonne of extra crop are converted to emissions per MJ biofuel from that crop. The average global deforestation caused by increase in production of a crop or biofuel is estimated, making no geographical differentiation in where the extra demand occurs or where that would provoke deforestation. The source of historical deforestation data is a report published by DG ENV [EC 2013] which estimates which areas of forest were lost to different crops and to other land uses (grazing, logged forest, urban and others.) between 1990 and 2008. It used historical deforestation data from FAO’s Forest Resource Assessment 2010, interpreted with other FAO data. The emissions are calculated only from deforestation and peat forest drainage, attributed to each MJ biofuel. This does not include emissions from the grassland area converted to cropland. This method gives an independent verification of the general magnitude of LUC area and emissions which should be expected from bottom-up models of LUC for scenarios, and the results indicate that historical LUC emissions were higher than those estimated by most economic models.
Outward Direct Investment, Firm Productivity and Credit Constraints: Evidence from Chinese Firms

China is currently the third largest country in terms of outward direct investment (ODI), with the investors mainly being state‐owned enterprises. This presents a question: What inhibits private enterprises from increasing ODI? Using a firm‐level panel data set for Zhejiang Province in China, we examine the impact of firm heterogeneity on private firm ODI. We have three main findings: first, a higher productivity level contributes to better access to ODI, and increases ODI value as well; second, lowering a firm's financial constraint level can increase both the probability and volume of ODI; third, productivity cannot offset the negative effect of financial constraint on private firm ODI.
ERINOKS: EneRgy-Efficient INduction-Based Food Processing for Optimized KitchenS

The new Industry 4.0 era has opened a new gateway having the capacity to be applied in gastronomy sector in order to provide clever solutions to its critical challenges or emerging needs like: Energy consumption; Process optimization; Healthy and personalized nutrition; and Effective maintenance and refinement of entire portfolio of gastronomic services. The proposed food technology, which is mainly based on so-called magnetic induction, is enabled as an embedded solution. The holistic solution, namely ERINOKS, has an Internet-of-Things (IoT) background enabling distributed and connected food processes, adaptive power control according to the amount of food (by weight control) and a recommendation system for effective nutrition of individuals or group of people having special preferences. ERINOKS supports statistical methods like bootstrapping to analyze the parameters affecting the food processes and energy efficiency. Such a statistical analysis can be used to model the food consumption rates according to age, gender, origin, daily or seasonal factors and individual preferences or health status. ERINOKS utilizes a virtual assistant simulating food preparation steps or just for sharing information about ingredients for the use of cookers and consumers, i.e. selecting or cooking the healthiest food.
THE UNOFFICIAL ECONOMY ? EVIDENCE FROM PANEL DATA

One factor that contributes in the size of the shadow economy is the regulation of business activities. This paper provides empirical analysis of the effects of regulatory discretion on the unoffi cial economy. It adds to the previous fi ndings by gathering evidence from a large data set of 162 countries for the 1999 to 2007 period. Going beyond simple correlation, it uses the Arellano–Bond estimator to investigate the dynamics and causal effects of regulation on the shadow economy. We fi nd that increase in regulation increases the size of the shadow economy.
Repertoire Shift in the Humoral Response to Phosphocholine-Keyhole Limpet Hemocyanin: VH Somatic Mutation in Germinal Center B Cells Impairs T15 Ig Function1

Phosphocholine (PC) is a naturally occurring Ag common to many pathogenic microorganisms. Early in the primary response to PC conjugated to keyhole limpet hemocyanin (KLH), T15 Id+ Abs constitute >90% of the serum Ig in BALB/c mice. During the late primary and memory response to PC-protein, a shift in the repertoire occurs and T15 Id+ Abs lose dominance. In this study, we use immunohistochemistry and single germinal center microdissection to locate T15 Id+ cells in the spleen in a primary response to PC-KLH. We demonstrate T15 Id+ B cells and VH1-DFL16.1-JH1 and Vκ22-Jκ5 rearrangements in germinal centers early in the immune response; thus loss of T15 dominance is not due to lack of T15 cells within germinal centers. One-hundred thirty one VH1 and 57 Vκ22 rearrangements were cloned and sequenced. Thirty four percent of the VH1 clones and 37% of the Vκ22 clones contained somatic mutations indicating participation in the germinal center response. Six variant T15 H clones were expressed with wild-type T15 L chain in vitro. Two of these Abs were defective in secretion providing the first evidence that mutation occurring in vivo can disrupt Ig assembly and secretion. Of the four secretion-competent Abs, two failed to display binding to PC-protein, while the other two displayed altered carrier recognition. These results indicate that somatic mutation of T15 in vivo can result in the loss of binding and secretion, potentially leading to B cell wastage. The failure of T15 to gain affinity enhancing mutations in the face of these detrimental changes may contribute to repertoire shift.
Vancomycin-Resistant Enterococci from Humans and Retail Chickens in Taiwan with Unique VanB Phenotype-vanA Genotype Incongruence

ABSTRACT Vancomycin resistant enterococci (VRE) with VanB phenotype-vanA genotype incongruence were found in all 39 VRE isolated from chicken carcasses and four human VRE isolates in Taiwan. Three identical mutations in the vanS gene were found in the VanB phenotype-vanA genotype VRE sequenced. This finding indicates possible transmission of glycopeptide resistance among different hosts.
Gene expression profiles in circulating tumor cells to predict prognosis in metastatic breast cancer patients.

BACKGROUND A circulating tumor cell (CTC) count is an established prognostic factor in metastatic breast cancer (MBC). Besides enumeration, CTC characterization promises to improve outcome prediction and treatment guidance. Having shown the feasibility of quantifying clinically relevant mRNA transcripts in CTCs, we determined the prognostic value of CTC gene expression in MBC. PATIENTS AND METHODS CTCs were isolated and enumerated from blood of 197 MBC patients who were about to start first-line systemic therapy. Of these, 180 were assessable for quantification of mRNA expression by RT-qPCR in relation to time-to-treatment failure (TTF). A prognostic CTC gene profile was generated by leave-one-out cross validation in a 103 patient discovery set and validated in 77 patients. Additionally, all 180 patients were randomly divided into two equal sets to discover and validate a second prognostic profile. RESULTS CTC count predicted for TTF at baseline {≥5 versus <5 CTCs/7.5 ml blood, hazard ratio (HR) 2.92 [95% confidence interval (CI) 1.71-4.95] P < 0.0001}. A 16-gene CTC profile was generated in the first discovery set, which identified patients with death or TTF <9 months versus those with a better outcome. In multivariate analysis, the 16-gene profile was the only factor associated with TTF [HR 3.15 (95% CI 1.35-7.33) P 0.008]. Validation of this profile in the independent patient set pointed into the same direction, but was not statistically significant. A newly generated 8-gene profile showed similarly favorable test characteristics as the 16-gene profile, but did not significantly pass validation either. CONCLUSION A 16-gene CTC profile was identified, which provided prognostic value on top of CTC count in MBC patients. However, validation of this profile in an independent cohort, nor of a second profile, reached statistical significance, underscoring the need to further fine-tune the still promising approach of CTC characterization.
Are Th17 cells and their cytokines a therapeutic target in Guillain–Barré syndrome?

Introduction: Guillain–Barré syndrome (GBS) is an immune-mediated inflammatory disorder of the peripheral nervous system (PNS). Experimental autoimmune neuritis (EAN) is a useful animal model for studying GBS. Currently, GBS remains a life-threatening disorder and more effective therapeutic strategies are in urgent need. Areas covered: Accumulating evidence has revealed that T helper (Th) 17 cells and their cytokines are pathogenic in GBS/EAN. Drugs attenuated clinical signs of GBS/EAN, in part, by decreasing Th17 cells or IL-17A. Th17 cells and their cytokines might be potential therapeutic targets. Approaches targeting Th17 cells or their cytokines are in development in treating Th17 cells-involved disorders. In this review, we summarize the up-to-date knowledge on roles of Th17 cells and their cytokines in GBS/EAN, as well potential approaches targeting Th17 cells and their cytokines as clinical applications. Expert opinion: As Th17 cells produce different sets of pro-inflammatory cytokines and Th17-related cytokines are not exclusively produced by Th17 cells, targeting Th17 cell development may be superior to blocking a single Th17 cytokine to treat Th17 cells-involved disorders. Considering the essential role of retinoic acid-related orphan receptor γT (RORγT) and IL-23 in Th17 cell development, RORγT inhibitors or IL-23 antagonists may provide better clinical efficacy in treating GBS/EAN.
Prevalence and Intensity of Nematode Parasites in Wisconsin Ermine

Abstract: In the midwestern United States, ermine (Mustela erminea) are economically important because they are legally harvested for pelts. Information on parasites of ermine is lacking, and the effects that nematode parasites have on body condition of ermine hosts are unknown. We identified Skrjabingylus nasicola and Filaroides martis in ermine trapped from 2007 to 2013 from 6 counties in Wisconsin. Small mammals, commonly consumed by ermine, serve as paratenic hosts for both parasites. Our goal was to identify how age and sex of ermine, along with year, influence nematode parasitism. We also investigated how infection affected body condition for male and female ermine using body mass standardized by length as an index of body condition. We commonly found S. nasicola and F. martis in male and female ermine, but both prevalence and intensity of infection were higher for males. Relative to juveniles (<1 yr), adult (>1 yr) male ermine did not exhibit significantly higher intensity or prevalence of either parasite. We found that body condition was not compromised by infection for either sex, and intensity of S. nasicola and prevalence of F. martis were highest during the 2010–2011 trapping season. Of the 6 yr studied, precipitation was highest during the summer before the 2010–2011 season, and increased precipitation can cause increases in populations of gastropod intermediate hosts. We think that several distinct natural history components, namely, mating structure, diet, and metabolic rate, influence nematode parasitism in ermine.
Growth in a Time of Debt

In this paper, we exploit a new multi-country historical dataset on public (government) debt to search for a systemic relationship between high public debt level growth and inflation our main result is that whereas the link between growth and debt seems relatively weak at “normal” debt levels, median growth rates for countries with over roughly ninety percent of gdp are about one percent lower than otherwise; average (mean) growth rates are several percent lower . surprisingly,
A COMPARISON OF FOUR SEROLOGIC ASSAYS IN SCREENING FOR BRUCELLA EXPOSURE IN HAWAIIAN MONK SEALS

A survey for Brucella spp. antibodies was undertaken on 164 serum samples from 144 Hawaiian monk seals (Monachus schauinslandi) from the northwestern Hawaiian Islands collected between 1995 and 2002. The buffered antigen plate agglutination test (BPAT), the indirect enzyme immunoassay (I-ELISA), the competitive enzyme immunoassay (C-ELISA), and the fluorescence polarization assay (FPA) were compared with regard to their ability in detecting antibodies to Brucella spp. in the serum samples. Overall, antibodies were detected in 28 (17.1%) animals, using the BPAT test, 25 (15.2%) by the C-ELISA, and 19 (11.6%) in the I-ELISA and the FPA test, using thresholds established for cattle. No evidence of gross pathology consistent with clinical brucellosis was noted in any of the seropositive animals tested. Although further work would be necessary to validate these tests for use with monk seals it appears that both the C-ELISA and the FPA tests would be appropriate as diagnostic screening tests for detection of antibodies to Brucella spp. in this species.
Different centre of pressure patterns within the golf stroke II: Group-based analysis

Abstract Although the golf coaching literature stresses the importance of weight transfer during the swing, research has been conflicting or lacking statistical support. A potential problem with previous studies is that no attempt was made to account for different movement strategies in the golf swing. This study evaluated the relationship between centre of pressure measures and club head velocity within two previously identified swing styles, the “Front Foot” and “Reverse” styles. Thirty-nine Front Foot golfers and 19 Reverse golfers performed swings with a driver while standing on two force plates. From the force plate data, centre of pressure displacement, velocity, range, and timing parameters were calculated. Correlation and regression analysis indicated that a larger range of centre of pressure and a more rapid centre of pressure movement in the downswing was associated with a larger club head velocity at ball contact for the Front Foot group. For the Reverse golfers, positioning the centre of pressure further from the back foot at late backswing and a more rapid centre of pressure transfer towards the back foot at ball contact was associated with a larger club head velocity at ball contact. This study has highlighted the importance of identifying different movement strategies before evaluating performance measures, as different parameters were found to be important for the Front Foot and Reverse styles.
The Rise of Agreement; the Case of Persian Enclitics

Traditionally, it was supposed that Persian pronominal enclitics act as pronouns or real arguments, while this paper tries to indicate that they act as agreement markers in some contexts, too. As historically they have moved from Wackernagel's position toward verb adjacent positions, their functions have also changed. Now, they are used as subject agreement markers in a group of Persian structures. It is argued that they have acquired this new role, subject agreement markers, via grammaticalization, and Blocking Principle, as envisaged by Fus (2005) could explain this process.
Diagnostic accuracy of serological assays in pediatric inflammatory bowel disease.

BACKGROUND & AIMS Accurate serological assays are desirable for the diagnosis of inflammatory bowel disease (IBD) types in the pediatric age group. The aim of this study was to test the diagnostic accuracy of modified assays for perinuclear (p) antineutrophil cytoplasmic antibodies (ANCAs) and anti-Saccharomyces cerevisiae antibodies (ASCAs) in patients with pediatric ulcerative colitis (UC) and Crohn's disease (CD) and in those without IBD. METHODS With observers blinded to patients' diagnoses, serum specimens were analyzed for immunoglobulin (Ig) A and IgG ASCAs and ANCAs by enzyme-linked immunosorbent assay. The perinuclear location of ANCAs visualized by indirect immunofluorescence was confirmed by its disappearance after administration of deoxyribonuclease. RESULTS IgA and IgG ASCA titers were significantly greater and highly specific for CD (95% for either, 100% if both positive). pANCA was 92% specific for UC and absent in all non-IBD controls. The majority of patients with CD positive for pANCA had a UC-like presentation. Disease location, duration, activity, complications, and treatment with immunosuppressive drugs did not have an impact on the ASCA or pANCA assay results. After resection, UC patients remained pANCA positive, in contrast to patients with CD, in whom ASCA titers decreased toward normal values postoperatively. CONCLUSIONS ASCA and pANCA assays are highly disease specific for CD and UC, respectively. These serological tests can assist clinicians in diagnosing and categorizing patients with IBD and may be useful in making therapeutic decisions.
Review: Efficacy and tolerability of olmesartan/amlodipine combination therapy in patients with mild-to-severe hypertension: focus on 24-h blood pressure control

European guidelines recommend that treating patients with hypertension to blood pressure (BP) goal is an important target for cardiovascular (CV) risk reduction. However, office BP may be a suboptimal target, given its limitations. Indeed, there is evidence that 24-h ambulatory BP monitoring (ABPM) parameters may score better in this regard, representing more accurate predictors of CV risk. In particular, mean 24-h BP and BP variability both correlate closely with hypertension end-organ damage and rate of CV events, which suggests that antihypertensive therapy should provide smooth BP control over the full 24-h dosing interval. The use of ABPM has demonstrated that fixed-dose combination therapy, comprising agents with complementary mechanisms of action, may overcome the challenge of suboptimal BP control by providing improvements in antihypertensive efficacy and tolerability throughout the 24-h period. Olmesartan/amlodipine is one of the latest combination therapies to be approved, and a number of large clinical trials have demonstrated the efficacy and tolerability of this combination in patients with mild-to-severe hypertension. Furthermore, recent ABPM studies of olmesartan/amlodipine-based treatment algorithms have shown the satisfactory 24-h antihypertensive efficacy of this fixed-dose combination. This review provides an overview of recent clinical data on the efficacy and tolerability of fixed-dose olmesartan/amlodipine combination therapy for the treatment of mild-to-severe hypertension, with a focus on sustained 24-h BP control.
Dose-response study of spinal hyperbaric ropivacaine for cesarean section

Background: Spinal hyperbaric ropivacaine may produce more predictable and reliable anesthesia than plain ropivacaine for cesarean section. The dose-response relation for spinal hyperbaric ropivacaine is undetermined. This double-blind, randomized, dose-response study determined the ED50 (50% effective dose) and ED95 (95% effective dose) of spinal hyperbaric ropivacaine for cesarean section anesthesia. Methods: Sixty parturients undergoing elective cesarean section delivery with use of combined spinal-epidural anesthesia were enrolled in this study. An epidural catheter was placed at the L1∼L2 vertebral interspace, then lumbar puncture was performed at the L3∼L4 vertebral interspace, and parturients were randomized to receive spinal hyperbaric ropivacaine in doses of 10.5 mg, 12 mg, 13.5 mg, or 15 mg in equal volumes of 3 ml. Sensory levels (pinprick) were assessed every 2.5 min until a T7 level was achieved and motor changes were assessed by modified Bromage Score. A dose was considered effective if an upper sensory level to pin prick of T7 or above was achieved and no intraoperative epidural supplement was required. ED50 and ED95 were determined with use of a logistic regression model. Results: ED50 (95% confidence interval) of spinal hyperbaric ropivacaine was determined to be 10.37 (5.23∼11.59) mg and ED95 (95% confidence interval) to be 15.39 (13.81∼23.59) mg. The maximum sensory block levels and the duration of motor block and the rate of hypotension, but not onset of anesthesia, were significantly related to the ropivacaine dose. Conclusion: The ED50 and ED95 of spinal hyperbaric ropivacaine for cesarean delivery under the conditions of this study were 10.37 mg and 15.39 mg, respectively. Ropivacaine is suitable for spinal anesthesia in cesarean delivery.
A Path Model of School Violence Perpetration: Introducing Online Game Addiction as a New Risk Factor

Drawing on the cognitive information–processing model of aggression and the general aggression model, we explored why adolescents become addicted to online games and how their immersion in online games affects school violence perpetration (SVP). For this purpose, we conducted statistical analyses on 1,775 elementary and middle school students who resided in northern districts of Seoul, South Korea. The results validated the proposed structural equation model and confirmed the statistical significance of the structural paths from the variables; that is, the paths from child abuse and self-esteem to SVP were significant. The levels of self-esteem and child abuse victimization affected SVP, and this effect was mediated by online game addiction (OGA). Furthermore, a multigroup path analysis showed significant gender differences in the path coefficients of the proposed model, indicating that gender exerted differential effects on adolescents’ OGA and SVP. Based on these results, prevention and intervention methods to curb violence in schools have been proposed.