Miracle-dz/UniFAR-FTUs huggingface dataset:
KLF5 Promotes Breast Cell Survival Partially through Fibroblast Growth Factor-binding Protein 1-pERK-mediated Dual Specificity MKP-1 Protein Phosphorylation and Stabilization*

Krüpple-like transcription factor 5 (KLF5) is a zinc-finger transcription factor promoting cell survival and tumorigenesis in multiple cancers. A high expression level of KLF5 has been shown to be associated with shorter breast cancer patient survival. However, the role of KLF5 and mechanism of KLF5 actions in breast cancer remain unclear. In this study, we found that KLF5 knockdown by small interfering RNA in two breast cell lines, MCF10A and BT20, induces apoptosis. Interestingly, a pro-survival phosphatase, dual specificity mitogen-activated protein kinase phosphatase 1 (MKP-1), is down-regulated by KLF5 ablation. Consistently, KLF5 overexpression increases the MKP-1 protein expression in Hs578T and MCF7. We further found that MKP-1 is essential and sufficient for KLF5 to promote breast cell survival. However, MKP-1 is not a KLF5 direct transcription target because the MKP-1 mRNA level is not regulated by KLF5. By cycloheximide chase assays, we found that KLF5 decreases MKP-1 protein degradation via activating the ERK signaling. Inhibition of pERK by the pharmacological inhibitor U0126 specifically blocks KLF5-induced MKP-1 phosphorylation and stabilization. Additionally, constitutive activation of ERK by constitutively activated MEK1 rescues the KLF5 depletion-induced MKP-1 down-regulation. Consistently, the phosphorylation-deficient MKP-1 mutant cannot be stabilized by KLF5. Finally, the activation of ERK by KLF5 is very likely through the KLF5 direct target gene FGF-BP in breast cells. These findings suggest that KLF5 is a pro-survival factor that promotes breast cell survival partially through pERK-mediated MKP-1 phosphorylation and stabilization. The KLF5-FGF-BP-pERK-MKP-1 signaling axis may provide new therapeutic targets for invasive breast cancer.
Long-term follow-up of Hodgkin's disease trial.

To the Editor: In 1992, we reported the results of the prospective, randomized Cancer and Leukemia Group B trial in patients with newly diagnosed, advanced-stage Hodgkin's disease. The study compared three regimens: MOPP (mechlorethamine, vincristine, procarbazine, and prednisone) for 6 to 8 months, the standard treatment at that time; ABVD, a nonalkylating-agent–containing regimen (doxorubicin, bleomycin, vinblastine, and dacarbazine) for 6 to 8 months; and MOPP alternating with ABVD for 12 months.1 Radiation therapy was not administered. ABVD therapy alone for 6 to 8 months resulted in a rate of failure-free survival similar to the rate with a 12-month regimen of .
High-dose ampicillin-sulbactam as an alternative treatment of late-onset VAP from multidrug-resistant Acinetobacter baumannii

The increased incidence of multidrug-resistant (MDR) Acinetobacter baumannii ventilator-associated pneumonia in critically ill patients poses a severe therapeutic problem. The aim of this study was to evaluate the efficacy and safety of 2 high-dose treatment regimens of ampicillin-sulbactam (A/S) for MDR Acinetobacter baumannii VAP. We undertook a randomized, prospective trial of critically ill patents with (MDR) Acinetobacter baumannii VAP. Patients were randomly assigned to 1 of 2 treatment regimens of A/S (at a rate 2:1 every 8 h): 1) group A, 18/9 g daily dose (n = 14); and 2) group B, 24/12 g daily dose (n = 13). The duration of therapy was 8±2 d for both groups. A total of 27 patients were enrolled in the study. Clinical improvement was seen in 66.7% of the study population in 9/14 (64.3%) of group A patients and 9/13 (69.2%) of group B patients, respectively. Bacteriological success was achieved in 77.8% of the study population (12/14, 85.7% of group A) and in 9/13 (69.2%) of group B patients. The 14-d mortality rate was 25.9% and the all cause 30-d mortality was 48.1%. Both mortality rates did not differ significantly between the 2 groups. No major adverse reactions were recorded. We concluded that clinical and bacteriological results of the study support the use of high-dose regimen of ampicillin-sulbactam for MDR Acinetobacter baumannii VAP.
A generic randomization device for quantitative response variables

ABSTRACT We propose a generic randomization device (RD) for the estimation of mean of quantitative sensitive characteristic that make use of additive and multiplicative scrambled responses with blank card strategy. For this generic RD, we work with 12 choices and derive statistical properties of each model and give many immediate results in corollaries. It is interesting to note that various existing models can now be viewed as the special cases of this RD. Finally, various empirical studies have been done for each of these models to evaluate the efficiency performance.
Experimental evaluation of co‐culture of juvenile sea cucumbers, Holothuria scabra (Jaeger), with juvenile blue shrimp, Litopenaeus stylirostris (Stimpson)

The co-culture of juvenile sea cucumber Holothuria scabra (Jaeger), or ‘sandfish’, with juvenile blue shrimp Litopenaeus stylirostris (Stimpson) was tested by growing groups in co-culture and monoculture for 3 weeks in tanks with enriched sand substratum. Feed was supplied on trays, accessible only to shrimp. Survival of shrimp and sandfish was high in all treatments (73–100%). Growth of shrimp did not differ between monoculture and co-culture, but sandfish grew significantly slower in co-culture (P=0.03), although their sand burying and surface foraging were apparently unaffected by shrimp (P=0.76). However, shrimp increased the levels of total ammonia-N in tanks, which related inversely with sandfish growth (P=0.04). Conversely, sandfish did not appear to lower the water quality for shrimp culture. While sandfish bioturbate sediments and eat organic deposits, the juveniles did not significantly reduce the organic content of sand in tanks. Co-culturing juveniles of the two species in earthen ponds appears feasible, with no detriment to shrimp production, presenting a cost-effective method for growing sandfish to larger sizes for restocking. These findings underpin further studies to test the viability of commercial co-culture of sandfish with blue shrimp at later stages in the production cycle of shrimp.
A critical evaluation of methods for prediction of protein secondary structures.

CONTENTS mSTORICAL DEVELOPMENT OF THE FIELD 1 Postu lation o f a. -He li x and fJ-P leated Sheets . 1 Lo ca l Order in Synthetic Po lypeptides . 2 Leve ls o f Pr otei n Stru cture . 2 DEFINITION OF SECONDARY STRUCTURE •••••••••• •••• 3 He li ces and Sheets as De fined by Hydrogen Bonds 3 Reverse Tu rns . 4 Coi l Co nformation. 5 Other Parameters . 6
Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation

ULK1 is a key kinase in autophagy initiation. Nazio et al. demonstrate that the E3 ubiquitin ligase NEDD4L targets ULK1 for degradation soon after autophagy induction, whereas a simultaneous ULK1 mRNA transcription is needed for priming subsequent rounds of autophagy.
Anterior segment complications in diabetic patients following extracapsular cataract extraction and posterior chamber intraocular lens implantation.

Recovery of the anterior segment following extracapsular cataract extraction and posterior chamber intraocular lens implantation in 112 diabetic eyes was compared to that in 108 eyes of nondiabetic patients who underwent a similar procedure. The incidence of postoperative anterior segment complications was significantly higher in the diabetic than in the nondiabetic patients. The main complications were pigment dispersion, appearance of fibrin, development of posterior synechiae, and--rarely, and only in diabetic patients--pupillary block and transient iridal vascular congestion. The fibrin resolved following increased administration of steroids by means of eye drops and subconjunctival injections, with or without the addition of systemic steroids. Women were found to be at greater risk for development of posterior synechiae (P less than .025). Accumulation of fibrin was seen more often in women and in patients with preexisting diabetic retinopathy than in other patients, although the difference was not significant.
Presidential Address, 1975. The cardiovascular-behavioral interaction--as it appears today.

Research is reviewed concerning the interrelationships among cardiodynamics, blood pressure control mechanisms, somatic activity, and the stimulus parameter of active vs passive coping. Emerging evidence suggests that with passive coping such as classical aversive conditioning, the heart is more under vagal control which is directionally linked with somatic activity, while blood pressure is more dominated by vascular processes. With active coping such as shock avoidance, the heart is under greater sympathetic control which is directionally independent of concomitant somatic activity, while cardiac influences on blood pressure become more dominant. Several current psycho physiological issues are discussed including the possible significance of these effects for cardiovascular disease processes.
Validity Evaluation of the Uncertain EMC Simulation Results

Uncertainty analysis is widely used in today's electromagnetic compatibility simulations in order to include the variation and tolerance caused by realistic nonidealities. However, previous research has neglected to investigate the validity evaluation of uncertainty analysis results. In this paper, a novel validity evaluation method, named mean equivalent area method (MEAM), is proposed to quantify the difference between the reference data and the simulation results, particularly for validity evaluation of the uncertainty analysis results in computational electromagnetics. Comparing with other existing works or confirmed conclusion in published references, the performance of MEAM is presented. Furthermore, in order to improve both efficiency and accuracy of the uncertainty analysis method (Monte–Carlo method or generalized polynomial chaos method), a scheme based on MEAM is presented to examine whether the uncertainty analysis method has reached convergence. Finally, the selection of the reference data and the limitation of MEAM are illustrated in detail in Section VI.
UvA-DARE (Digital Academic Repository) Neutralization or Truncation? The perception of two Russian pitch accents on utterance-final syllables

This paper presents the results of a perception experiment that was carried out to verify the hypothesis that in Russian the contrast between pitch accents LH*L and LH* on utterance-ﬁnal syllables is neutralized. Recordings for the experiment were 10 sets of three short utterances with word stress in the ultimate, penultimate and antepenultimate syllable of the utterance-ﬁnal word. These utterances were read aloud by four female and four male native speakers. They were asked to realize accents LH*L and LH* in the utterance-ﬁnal word. After instructions and rehearsing, recordings were made separately for each of the two types. In the perception experiment, 30 native subjects listened to short utterances selected from the recordings and presented in 180 pairs: 120 pairs with ultimate stress and, in order to test whether listeners can hear the diﬀerence at all, 60 pairs with penultimate and antepenultimate word stress in utterance-ﬁnal position. The 180 stimuli pairs consisted of short utterances with realizations of LH*L and LH* on the ﬁnal word, each pair containing two same or two diﬀerent types of pitch accent. The task was to compare two stimuli in a pair and to indicate on a score form whether two realizations in a stimulus pair count as passable imitations of each other and thus belong to the same type of pitch accent. The same/diﬀerent judgments indicate that listeners suc-cessfully distinguished between the two pitch accents in the antepenultimate and penultimate conditions, but much less so in the ultimate condition. This suggests that the two accents are truncated in ﬁnal position, but not neutralized.
Indications and Contraindications: Interspinous Process Decompression Devices in Lumbar Spine Surgery

Abstract Background Interspinous process decompression devices (IPD) allow a minimally invasive treatment of lumbar spinal stenosis (LSS), but their use is discussed highly controversial. Several level I studies suggest that IPD implantation is a viable alternative for both conservative treatment and decompression, but clear indications and contraindications are still missing. This study was designed to explore the perspectives and limitations of IPDs and to evaluate the role of these devices in general. Material and Methods The study is based on a questionnaire sent to all hospitals registered in the German Hospital Address Register 2010 with an orthopedic, neurosurgerical, or spine surgery department (n = 1,321). The questionnaire was reviewed by experienced spine surgeons and statisticians, and included both single-response, close-ended, and multiple-response open-ended questions. Results We received 329 (24.9%) entirely analyzable questionnaires. A total of 164 respondents (49.8%) stated that IPDs are a treatment option for LSS, and 135 of the 164 respondents (82.3%) use them. Poor clinical experience (60%) and lack of evidence (53.9%) are the main reasons cited for not using IPDs. We detected a high negative correlation between the size of the hospital, the number of outpatients and inpatients treated for LSS and other spine pathologies, and the use of IPDs (p = 0.001). Most respondents prefer the combination of open decompression and IPD (64.4%; n = 87). A total of 9.6% (n = 13) of the users favor IPD implantation as a stand-alone procedure. Overall, 25.9% n = 35 use both options. Most surgeons aim to relieve the facet joints (87.7%) and to stabilize a preexisting instability (75.4%). They recommend IPDs in the segments L2–L3 (77%), L3–L4 (98.5%), and L4–l5 (99.3%) and consider that IPD implanation also could be done at the L5–S1 segment (40.1%). Overall, 64.4% (n = 87) of the users recommend limiting IPD implantation to two segments. Infection (96.3%), fracture (94.8%), isthmic spondylolisthesis (77%), degenerative spondylolisthesis (higher than Meyerding I [57%]), lumbar spine scoliosis (48.1%), and osteoporosis (50.4%) are seen as contraindications for IPD. Conclusion No clear consensus exists among spine surgeons concerning the use of IPD for LSS treatment. The study showed that hospital-related parameters also influence decision making for or against the use of IPDs. However, despite the lack of evidence, the indications and contraindications which had been identified in the present study might contribute to improved outcomes after IPD implantation or at least prevent harm to patients.
MUSCULAR DYSTROPHY IN NORTHERN IRELAND *

Summary Sib pairs from twenty-six Northern Ireland families with at least one member affected by ‘autosomal limb-girdle muscular dystrophy’ have been examined for linkage between the dystrophy and sex, colour vision, taste sensitivity to phenylthiocarbamide, ABO, Rhesus, Lewis (a), P, MNS and salivary secretion of AB antigens. The data present no definite signs of linkage; the only significant score obtained by Penrose's sib-pair method was for Lewis (a) in 128 sib pairs from families in which affected members had no face involvement. In view of the small amount of information available in this isolated instance and the relatively large number of tests made, the evidence for linkage with Lewis (a) in this group of families is unconvincing.
Primary Malignant Lymphoma Originating from the Chest Wall without Preceding Pleural Disease

An 84-year-old woman presented to our hospital with dyspnea on exertion and left back pain. Chest X-ray and chest computed tomography (CT) revealed an irregular pleural mass invading her left chest wall with rib destruction and pleural effusion. CT-guided needle biopsy revealed diffuse large B-cell lymphoma. Low-dose oral etoposide produced a complete response, and she continued oral chemotherapy for one year after the diagnosis and maintained good performance status. We herein report a very rare case of non-pyothorax-associated lymphoma that nonetheless resulted in great recovery.
Effects of Dietary Citric Acid on Metabolic Indicators and Gene Expression in the Skeletal Muscles of Fasted Mice

Citric acid is recognized and utilized as a dietary supplement for its ability to eliminate fatigue. However, the molecular and cellular mechanism underlying this effect has not been elucidated. This study investigated the effect of intragastric citric acid administration on the energy metabolism and gene expression in the skeletal muscles of mice that had been fasted without exercise, using biochemical analyses of metabolic indicators and DNA microarrays. Expression of the genes involved in the glycolysis, tricarboxylic acid cycle and β-oxidation of fatty acids were not affected by citric acid administration. However, citric acid increased the level of blood glucose and expression of the phosphoenolpyruvate carboxykinase gene. These results indicate that citric acid ingestion may eliminate fatigue by promoting gluconeogenesis.
Venues in Social Media: Examining Ambiance Perception Through Scene Semantics

We address the question of what visual cues, including scene objects and demographic attributes, contribute to the automatic inference of perceived ambiance in social media venues. We first use a state-of-art, deep scene semantic parsing method and a face attribute extractor to understand how different cues present in a scene relate to human perception of ambiance on Foursquare images of social venues. We then analyze correlational links between visual cues and thirteen ambiance variables, as well as the ability of the semantic attributes to automatically infer place ambiance. We study the effect of the type and amount of image data used for learning, and compare regression results to previous work, showing that the proposed approach results in marginal-to-moderate performance increase for up to ten of the ambiance dimensions, depending on the corpus.
Dietary protein hydrolysate and trypsin inhibitor effects on digestive capacities and performances during early-stages of spotted wolffish : Suggested mechanisms

Growth rate is dependent upon adequate provision of amino acids especially in newly-hatched fish which experience very high growth rate. The replacement of a fraction of protein content by partially hydrolyzed (pre-digested) proteins was carried out and the digestive capacities and performances of larval/juvenile spotted wolffish (Anarhichas minor) were measured. The goal of this study was to verify whether the scope for growth is principally dictated by the proteolytic capacity of the digestive system by examining the effect of protein hydrolysates (PH) and trypsin inhibitor dietary inclusion on protein digestion/assimilation capacities, growth and survival. Four experimental diets were examined: C (control) I (supplemented with 750 mg/kg soybean trypsin inhibitor (SBTI)) H (supplemented with 20% PH) and HI (supplemented with 20% PH and 750 mg/kg SBTI). Protein hydrolysate supplementation gave significantly higher body mass than control at day 15 post-hatching. Unexpectedly, at day 30 and 60, fish administered diet HI (containing trypsin inhibitor) were heavier than the other groups. Suggested mechanisms are presented and discussed. The main conclusions of this study are that wolffish larval stage lasts roughly 15 days and that juvenile growth is linked to proteolytic capacity, but also very likely to absorption capacity of peptides and amino acids.
Women in the radiology profession: data from a 1995 national survey.

OBJECTIVE This report provides an in-depth picture of similarities and differences in the professional and practice characteristics of women and men who are radiologists in the United States, with specific attention to whether gender differences are less pronounced among younger radiologists. MATERIALS AND METHODS Data were taken from a national stratified random sample survey of radiologists. The response rate was 75%. The 1731 men and 294 women responding included diagnostic radiologists, radiation oncologists, and radiology-related nuclear medicine specialists. RESULTS Seven percent of respondents who completed their radiology residency before 1970 were women, compared with 9% in 1970-1979, 19% in 1980-1989, and 27% in 1990-1995. Gender differences were smaller among posttraining radiologists younger than 40 years old than among those 40 years old or older for the following questions: when the respondent decided to specialize in radiology, whether the respondent is a subspecialist within diagnostic radiology, whether the respondent's practice is primarily academic, whether the practice is privately owned, and whether the respondent is a practice owner (partner, shareholder). Women who were residents and fellows more frequently indicated problems with unwanted sexual attention occurring in the past 2 years than did women who were not in training positions. However, posttraining women were more likely to have encountered discrimination in salary, hiring, or promotion at some time in their careers. CONCLUSION Women's professional roles in radiology are changing slowly. A narrowing gender gap among younger radiologists on some professional and practice characteristics may indicate a further diminishing of gender differences in radiology in the future. It may also reflect greater gender equality at earlier rather than later stages of one's career.
Weed Management in Strip-tilled Irish Potato and Sweetpotato Systems

Experiments were conducted to evaluate the effect of tillage systems and weed management on weed suppression and potato yield. Striptillage (ST) and conventional-tillage (CT) systems produced equal yields of Irish potato (Solanum tuberosum L.) or sweetpotato [Ipomoea batatas (L.) Lam. ] when herbicide treatments were applied. Weeds in the nontreated control reduced yield of Irish potato and prevented storage root growth in sweetpotato. Excellent control of broadleaf signalgrass [Brachiaria platyphylla (Griseb.) Nash], henbit (Lamium amplexicaule L.), prickly sida (Sida spinosa L.), and common ragweed (Ambrosia artemisiifolia L.) was obtained with metribuzin + metolachlor applied preemergence at Irish potato planting, followed by sethoxydim + crop oil applied postemergence in ST and CT systems. Redroot pigweed (Amaranthus retroflexus L.) control was >98% at 4 weeks after treatment but was 73% to 84% at harvest across all herbicide treatments in both tillage systems. In sweetpotato, control of black mustard [Brassica nigra (L.) W.J.D. Koch], goosegrass [Eleusine indica (L.) Gaertn. ], and fall panicum [Panicum dichotomiflorum Michx. ] was >95% throughout the growing season for all herbicide treatments in both ST and CT.
Armed Conflict and Domestic Violence: Evidence from Rwanda

The 1994 Rwandan genocide deeply aected the structure of the population by decreasing the sex ratio, the relative number of men and women. In addition, those living in urban areas with an educated background were more likely to be killed during the mass slaying, which resulted in a loss of human capital (De Walque and Verwimp, 2010). This study uses data from the 2005 Rwanda Demographic and Health Survey and employs an empirical strategy similar to dierences-in-die rences to study how the demographic changes caused by the genocide aected domestic violence and other marriage outcomes. I find that women who got married after the genocide in provinces where the sex ratio decreased more were more likely to become victims of intimate partner violence. I also find that they married less educated husbands. The results are consistent with a one-sided spousal search model, where women lower their reservation values as a consequence of the scarcity of men and the deterioration of the quality of potential husbands caused by the genocide.