Miracle-dz/UniFAR-FTUs huggingface dataset:
The combination of bevacizumab/Avastin and erlotinib/Tarceva is relevant for the treatment of metastatic renal cell carcinoma: the role of a synonymous mutation of the EGFR receptor

Metastatic clear cell renal cell carcinomas (mRCC) over-express the vascular endothelial growth factor (VEGF). Hence, the anti-VEGF antibody bevacizumab/Avastin (BVZ) combined with interferon alpha (IFN) was approved for the treatment of mRCC. However, approval was lost in July 2016 due to the absence of sustained efficacy. We previously showed that BVZ accelerates tumor growth in experimental models of mRCC in mice, results in part explained by down-regulation of the phospho tyrosine phosphatase receptor kappa (PTPRκ) in tumor cells. The epidermal growth factor receptor (EGFR) is a direct target of PTPRκ. Its down-regulation leads to constitutive activation of EGFR, an observation which prompted us to test the effect of the EGFR inhibitor erlotinib/Tarceva (ERLO) in addition to BVZ/IFN. The influence of the long non-coding RNA, EGFR-AS1, on ERLO efficacy was also addressed. Methods: The effect of BVZ/IFN/ERLO was tested on the growth of experimental tumors in nude mice. The presence of germline mutation in the EGFR was evaluated on cell lines and primary RCC cells. In vitro translation and transfections of expression vectors coding the wild-type or the EGFR mutated gene in HEK-293 cells were used to test the role of EGFR mutation of the ERLO efficacy. Correlation between EGFR/EGFR-AS1 expression and survival was analyzed with an online available data base (TCGA). Results: Tumor growth was strongly reduced by the triple combination BVZ/IFN/ERLO and linked to reduced levels of pro-angiogenic/pro-inflammatory cytokines of the ELR+CXCL family and to subsequent inhibition of vascularization, a decreased number of lymphatic vessels and polarization of macrophages towards the M1 phenotype. Cells isolated from surgical resection of human tumors presented a range of sensitivity to ERLO depending on the presence of a newly detected mutation in the EGFR and to the presence of EGFR-AS1. Conclusions: Our results point-out that the BVZ/IFN/ERLO combination deserves testing for the treatment of mRCC that have a specific mutation in the EGFR.
Improvement of Malignant Glioma Treatment: Preclinical evaluation of adenoviral gene therapy and PRRT

textabstractGlioblastoma multiforme (GBM) is the most aggressive type of malignant glioma (WHO grade 4). Patients suffering from a GBM have a median survival of less than a year after diagnosis despite the recent developments in neurosurgery, radiotherapy and chemotherapy. This poor prognosis has led to the investigation of new treatment modalities. Locoregional treatment modalities are potentially attractive for GBMs because these tumors are generally confined to the central nervous system and only rarely metastasize outside the nervous system. Examples of such locoregional treatment modalities are gene therapy and peptide receptor radionuclide therapy (PRRT). As will be discussed in chapter 1.2, after successful gene therapy treatment experiments in animal models, the clinical results of GBM gene therapy have been disappointing, showing the need for improvement of this modality. PRRT could also be beneficial for GBM patients since small radiolabeled peptide molecules can migrate from the injection site to the tumor, and target to their corresponding receptor on the cell membrane of these cells. At this moment, however, it is not clear which peptide receptors are expressed on human GBM tissues. The goal of this thesis was to identify bottlenecks limiting the efficacy of glioma gene therapy using molecular imaging techniques. In addition, we explored the possibility of PRRT treatment for GBM patients.
Levantamento etnobotânico de plantas medicinais utilizadas na cidade de Vilhena, Rondônia / Ethnobotanical survey of medicinal plants used in the city of Vilhena, Rondônia

Este trabalho teve como objetivo fazer o levantamento etnobotânico de plantas medicinais no Municipio de Vilhena-RO. Foram aplicados questionarios estruturados a 99 moradores, identificando-se as plantas utilizadas, a finalidade terapeutica, os orgaos vegetais e a forma de preparo dos fitoterapicos. Alem disso, identificou-se tambem a forma de aquisicao dos conhecimentos acerca das plantas e o grau de escolaridade dos entrevistados. Identificou-se 42 especies de plantas medicinais, distribuidas em 22 familias botânicas, com maior representatividade para a familia Lamiaceae. As plantas mais citadas foram: boldo (Peumus boldus M.), hortela (Mentha pullegium L.), erva-cidreira (Lippia alba (Mill.) NE Brown), poejo (Mentha pulegium L.), erva doce (Pimpinella anisum L.), alecrim (Rosmarinus officinalis L.), capim santo (Cymbopogon citratus D.C.), laranja (Citrus sinensis (L.) O. ) e babosa (Aloe vera (L.) Burn. F). As partes mais utilizadas foram as folhas; sendo o fervimento o modo de preparo mais usual. As doencas mais citadas foram gripe, dores estomacais e calmantes. Diversas pesquisas sobre a utilizacao de plantas em tratamento terapeuticos tem sido realizadas. Entretanto, ainda ha muito a se conhecer sobre o uso terapeutico, eficacia e seguranca comprovada dos produtos derivados de plantas. O estudo das plantas medicinais e de grande relevância, tendo em vista a utilizacao das substâncias ativas no preparo de novos farmacos. Palavras-chave: Medicina natural. Amazonia. Lamiaceae. ABSTRACT: In this study we aim at making ethnobotanical survey of medicinal plants in the city of Vilhena, RO. Structured questionnaires were applied to 99 residents, identifying the plants used, the therapeutic purpose, the plant organs and how to prepare the herbal drugs. In addition, we identified also the way of acquiring knowledge about the plants and the level of education of respondents. We identified 42 species of medicinal plants distributed in 22 botanical families, with greater representation for the family Lamiaceae. The plants most frequently mentioned were: Boldo (Peumus boldus M.), peppermint (Mentha pullegium L.), lemon grass (Lippia alba (Mill.) NE Brown), pennyroyal (Mentha pulegium L.), anise (Pimpinella anisum L .), rosemary (Rosmarinus officinalis L.), holy grass (Cymbopogon citratus DC), orange (Citrus sinensis (L.) O. ) and aloe (Aloe vera (L.) Burn. F). The parts used were leaves, even boiling being the most usual method of preparation. The diseases most frequently mentioned were the flu, stomach aches and calming. Several studies on the use of therapeutic treatment plants have been carried out. However, there is still much to learn about the therapeutic use, proven efficacy and safety of products derived from plants. The study of medicinal plants is of great importance in view of the use of active substances in the preparation of new drugs. Keywords: Natural Medicine. Amazon. Lamiaceae.
A Coherent Framework for Predicting Emerging Market Credit Spreads with Support Vector Regression

We propose a coherent framework using support vector regression (SRV) for generating and ranking a set of high quality models for predicting emerging market sovereign credit spreads. Our framework adapts a global optimization algorithm employing an hv-block cross-validation metric, pertinent for models with serially correlated economic variables, to produce robust sets of tuning parameters for SRV kernel functions. In contrast to previous approaches identifying a single "best" tuning parameter setting, a task that is pragmatically improbable to achieve in many applications, we proceed with a collection of tuning parameter candidates, employing the Model Confidence Set test to select the most accurate models from the collection of promising candidates. Using bond credit spread data for three large emerging market economies and an array of input variables motivated by economic theory, we apply our framework to identify relatively small sets of SVR models with su perior out-of-sample forecasting performance. Benchmarking our SRV forecasts against random walk and conventional linear model forecasts provides evidence for the notably superior forecasting accuracy of SRV-based models. In contrast to routinely used linear model benchmarks, the SRV-based models can generate accurate forecasts using only a small set of input variables limited to the country-specific credit-spread-curve factors, lending some support to the rational expectation theory of the term structure in the context of emerging market credit spreads. Consequently, our evidence indicates a better ability of highly flexible SVR to capture investor expectations about future spreads reflected in today's credit spread curve.
Fire protection of GFRP pultruded profiles for floors of buildings

This paper presents the results of an experimental research on the fire behaviour of GFRP pultruded profiles, whose main objective was to study the viability of their use in floors of building, as structural elements. The feasibility and efficacy of using different protective coatings/layers and a water cooling system to provide fire protection to GFRP pultruded profiles was investigated. Dynamic mechanical analysis (DMA) and differential scanning calorimetry (DSC) and thermogravimetric (DSC/TGA) measurements were first performed in the GFRP material and also in the fire protection materials in order to determine their thermophysical and thermo-mechanical properties. Fire resistance tests were then conducted on an intermediate scale oven to investigate the behaviour of loaded GFRP pultruded beams in a fire situation (unprotected and protected), simulated by the ISO 834 time-temperature curve. These tests allowed to investigate the thermal response of the GFRP material when exposed to fire, the mechanical response and the failure modes of the beams under a simulated fire and the fire resistance of the beams with the different fire protection systems, allowing to define the field of application of each investigated solution, according to standards’ requirements.
PENGETAHUAN IBU HAMIL TENTANG INISIASI MENYUSU DINI

Abstract : Early breastfeeding initiation is babies’ immediate self-suckle within an hour after being born. However, according to one of the aforetime researches, only 4% women in Indonesia did early breastfeeding initiation, while the other 96% ones did not. Objective: to observe the expectant mothers’ cognition about the early breastfeeding initiation in polyclinic gynecologist/Obstetrics BLU RSU Prof. Dr. R. D. Kandou Manado, periode November-December 2013. Method: this research use descriptive research design with cross-sectional study approach . This research was carried out in November-December, 2013. The sample of the study was 50 expectant mothers checking their pregnancy at polyclinic gynecologist/Obstetrics BLU RSU Prof. Dr. R. D. Kandou Manado. Results: it was found that the associate knowledge upon early breastfeeding initiation according to the age group, exist in the age group <20 year (100%), according to the level of education, exist in university level (100%), and according to the number of children, exist in expectant mother with one child (100%). Conclusion : Overall, expectant mothers’ cognition upon early breastfeeding initiation in good category (96%), while in bad category (4%). Keywords : expectant mothers’ cognition, early breast feeding initiation. Abstrak: Inisiasi Menyusu Dini (IMD) adalah segeranya bayi menyusu sendiri dalam satu jam pertama setelah lahir. Namun menurut salah satu survei yang telah dilakukan, pelaksanaan inisiasi menyusu dini hanya dilakukan oleh 4% wanita di Indonesia sedangkan 96% wanita lainnya tidak mempraktekkannya. Tujuan: Mengetahui pengetahuan ibu hamil tentang inisiasi menyusu dini di Poliklinik Kandungan/Obstetri BLU RSU Prof. Dr. R. D. Kandou Manado periode November-Desember 2013. Metode: Penelitian ini menggunakan desain penelitian deskriptif dengan pendekatan cross-sectional study . Penelitian ini dilakukan pada bulan November-Desember 2013 dengan sampelnya adalah ibu hamil yang memeriksakan kehamilannya ke Poliklinik Kandungan/Obstetri BLU RSU Prof. Dr. R. D. Kandou Manado yang berjumlah 50 orang. Hasil: Didapatkan hasil pengetahuan yang baik tentang inisiasi menyusu dini menurut kelompok umur ada pada kelompok umur <20 tahun (100%), menurut pendidikan terakhir ada pada tingkat perguruan tinggi (100%) dan menurut jumlah anak ada pada ibu hamil yang memiliki satu anak (100%). Simpulan: Secara keseluruhan, pengetahuan ibu hamil tentang inisiasi menyusu dini dalam kategori baik (96%) sedangkan untuk kategori buruk (4%). Kata kunci: Pengetahuan ibu hamil, inisiasi menyusu dini.
Chlorine decay under steady and unsteady-state hydraulic conditions

This paper describes a simulation framework for the scale-adaptive hydraulic and chlorine decay modelling under steady and unsteady-state flows. Bulk flow and pipe wall reaction coefficients are replaced with steady and unsteady-state reaction coefficients. An unsteady decay coefficient is defined which depends upon the absolute value of shear stress and the rate of change of shear stress for quasi-unsteady and unsteady-state flows. A preliminary experimental and analytical investigation was carried out in a water transmission main. The results were used to model monochloramine decay and these demonstrate that the dynamic hydraulic conditions have a significant impact on water quality deterioration and the rapid loss of disinfectant residual. © 2013 The by Elsevier Ltd. Selection and peer-review under responsibility of the CCWI2013 Committee.
Mortalin stabilizes CD151-depedent tetraspanin-enriched microdomains and implicates in the progression of hepatocellular carcinoma

Background: Our previous studies showed that tetraspanin CD151 was implicated in the progression of hepatocellular carcinoma (HCC), mainly depending on the formation of functional complexes with molecular partners, including Mortalin. In this study, we investigate the role of mortalin in CD151-depedent progression of HCCs. Methods: Immunofluorescent staining, western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were used to investigate the expression and location of CD151 and Mortalin in four HCC cell lines with different metastatic ability. The relationship between Mortalin and CD151 was investigated in HCCLM3 cells using co-immunoprecipitation. CD151 or Mortalin expression in HCC cells were modified by transfection technology. Wound-healing assay and Transwell assay were used to assay the role of CD151 and Mortalin in cell migration and invasion. The expression and prognostic implication of CD151 and Mortalin in 187 cases of HCCs were analyzed. Results: Expression of Mortalin in HCC cells was positive related to their metastatic ability and its tendency was in line with the expression of CD151. Immunofluorescent staining showed that Mortalin was located in cytoplasm, while positive staining for CD151 was observed in cytoplasm and membrane of HCC cells. co-IP revealed that Mortalin formed a complex with CD151. Down-regulation of Mortalin induced a moderate decreased CD151 protein, but not CD151 mRNA, while inhibition of CD151 did not influence the expression of Mortalin at the level of both protein and mRNA. Interference of Mortalin significantly inhibited the invasion and migration of HCC cells with high CD151 expression and partially restored the invasion and migration of HCC cells induced by CD151 over-expression. Clinically, high Mortalin expression correlated with malignant phenotype of HCC, such as microvascular invasion (p=0.017) and tumor diameter (p=0.001). HCC patients expressing high Mortalin were tend to have higher expression of CD151. HCC patients expressing high level of CD151 showed the poorer prognosis in a Mortalin-dependent manner. Conclusions: Mortalin maybe stabilize of the structure of CD151-dependent tetraspanin-enriched microdomains and implicate in the progression of HCC.
The metabolic syndrome of omega-3-depleted rats.IX.Food intake

The present study complements recent investigations on the changes in fatty acid content and pattern of liver phospholipids and triglycerides found in � 3-depleted rats which, when exposed for 2 weeks to a flaxseed oil-enriched diet, display aggravated liver steatosis, visceral obesity and excess body weight. In the present experiments, the � 3- depleted rats exposed to the flaxseed oil-enriched diet still displayed insulin resistance, with higher plasma glucose and insulin concentrations than in control animals undergoing the same dietary manipulations. The food intake and gain in body weight after the switch in diet were also higher in the � 3-depleted rats than in control animals exposed for the same period to either soybean or flaxseed oil-enriched diets, there being a close analogy between these two variables in the 3 groups of rats. Emphasis is placed on the orexigenic effect attributable to the brain accumulation of C22:5� 3 and C22:6� 3, on the flaxseed dietary self-selection in the � 3-depleted rats, and on the increase in the lipid content of the diet from 5 to 10% (w/w) as possible determinants of the increase in food intake. In a clinical perspective, these findings indi- cate that, when considering the most suitable dietary approach to correct a deficiency in long-chain polyunsaturated � 3 fatty acids, equal attention should be paid to the lipid and � 3 fatty acid content of the diet, as well as to food intake, in or- der to avoid undesirable increases in adipose tissue mass and body weight.
Collaborating to compete

Collaboration – working together, in simple terms – is of great importance in diverse, multi-player supply networks such as those operated by Australian winemaking firms. This holds whether the collaboration is between employees fulfilling similar roles within an organisation, between specialists such as viticulturists and winemakers undertaking different roles, between people from different organisations, or even at a high-level between organisations. Fundamental drivers are the common goal of maximising the value realised from assets in the supply network (in particular the grape assets), the critical need for specialised knowledge, and the geographic and organisational diversity of participants. Harvesting and grape intake proceed most effectively if the operations of grape growers, harvester operators, grape transporters and wineries are tightly coordinated, are resilient to disruptions and can adapt to unplanned events. The benefits of collaboration are clear and significant. In this paper, we look in some detail at collaborative mechanisms for intake management and computerised decisionsupport applications that can promote such collaboration.
Relationship between adolescent varicocele and mean platelet volume Adölesan varikosel ile ortalama trombosit hacminin ilişkisi

Bulgular: Ocak 2012 ve Şubat 2018 tarihleri arasında kliniğimize başvuran 102 olgu çalışmaya dahil edildi. Olguların yaş ortalaması 15.12 ± 2.12 yıl idi. Her iki grup arasında yaş ortalamaları ve dağılımları açısından istatistiksel farklılık izlenmedi (p>0.05). Grup 1 ve 2 için yaş ortalamaları sırası ile 15.07±2.18 ve 15.19±2.06 idi. Grup 1 ve 2 için MPV değerler sırası ile 8.09±0.69 ve 9.65±1.6 olarak hesaplandı. Grup 2’de ölçülen MPV değeri grup 1 göre istatiksel olarak anlamlı olarak yüksek izlendi (p<0.001).
Arterial oxygen saturation and heart rate in term newborn in the first hour after birth.

INTRODUCTION The pulse oximeter has been useful in the cardiorespiratory evaluation of the newborn. OBJECTIVE To assess arterial oxygen saturation (SpO2) and heart rate (HR) in newborns in the first 60 minutes after birth. PATIENTS AND METHOD Prospective observational study in healthy term newborns, delivered vaginally or by cesarean section, with maternal bonding, carried out at sea level. A continuous post- ductal SpO2 and HR record were obtained from minute 1 to 10 after birth, and then at 15, 30 and 60 minutes. The SpO2 and HR were measured with a Nellcor pulse oximeter. The software Stata v.14 was used for the statistical analysis. RESULTS 324 healthy term newborns that met the inclusion criteria were included, of which 160 born vaginally and 164 by cesarean section. The SpO2 increased progres sively from minute 1 (58.7%) to minute 10 (94.5%). Newborns delivered vaginally had a significantly higher SpO2 until minute 10 after birth than those born by cesarean section (p < 0.001). In newborns delivered vaginally, HR was significantly higher in the first two minutes after birth, and then from minute 10 to 60 (p < 0.003). There were no differences by gender in SpO2 and HR. CONCLUSION In term newborns, the SpO2 increases progressively, being higher in the first 10 minutes in those born vaginally. In newborns delivered vaginally, a higher HR was also observed in the first and last minutes evaluated.
Contingent Valuation Method – Case examples from Czech Republic

In the Czech Republic (CR), the forest is generally accepted as a multifunctional natural resource, which is both a production and non-production factor in the life of the society; it is the natural wealth of the society but also wealth reproducible by labour. The economic, environmental and social importance of forest comes from a wide range of both market and non-market services. In the Czech Republic, forests are considered as a very important socioeconomic and environmental phenomenon, covering 33.8% of the total area of CR (Report, 2016). The area of forests has been gradually increasing over a long period from 2.191 mil. ha in 1875 and 2.479 mil. ha in 1950 to 2.662 mil. ha in 2013 in spite of big deforestations for infrastructure and artificial lakes building, natural raw materials mining, industry and settlements enlarging, and is gradually still increasing (mostly by afforestation of abandoned agricultural lands). In 2012, forest stands covered 98.2% of the total forestland area, which demonstrates a relatively high density of forests and percentage of forestland used for the forest services’ providing (Report 2014). Forests are almost regularly scattered all over the country, easily and freely accessible to people irrespective of forest ownership kind without any general limits (there are only several specific limits for forest visiting, e.g. in nature strong protected areas, drinking water reservoirs perimeters, and military areas). The beginnings of forest services valuations dates back to the 1970s and 1980s in the CR. Generally, the methodological approaches were focused to both expert valuation methods and consumer surplus valuation methods (mainly CVM – Willingness to pay variant). Generally, particular valuation methods and techniques have been chosen by:  aims of forest services valuation,  relationships of individual forest services to the market: market forest services, non-market forest services influencing the market (saving financial means of the society), pure non-market forest services (so called social forest services),  input data availability.
On the projectivity of proper normal curves over valuation domains

A theorem of S. Lichtenbaum states, that every proper, regular curve C over a discrete valuation domain R is projective. This theorem is generalized to the case of an arbitrary valuation domain R using the following notion of regularity for non-noetherian rings introduced by J. Bertin: the local ring OC,x of a point x ∈ C is called regular, if every finitely generated ideal I has finite projective dimension. The generalization is a particular case of a projectivity criterion for proper, normal R-curves: such a curve C is projective if for every irreducible component Y of its closed fibre Cm there exists a closed point P of the generic fibre of C such that the Zariski closure P meets Y , and meets Cm in regular points only.