Miracle-dz/UniFAR-FTUs huggingface dataset:
The key protein of endosomal mRNP transport Rrm4 binds translational landmark sites of cargo mRNAs

RNA-binding proteins (RBPs) determine spatiotemporal gene expression by mediating active transport and local translation of cargo mRNAs. Here, we cast a transcriptome-wide view on the transported mRNAs and cognate RBP binding sites during endosomal messenger ribonucleoprotein (mRNP) transport in Ustilago maydis. Using individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP), we compare the key transport RBP Rrm4 and the newly identified endosomal mRNP component Grp1 that is crucial to coordinate hyphal growth. Both RBPs bind predominantly in the 3’ untranslated region of thousands of shared cargo mRNAs, often in close proximity. Intriguingly, Rrm4 precisely binds at stop codons, which constitute landmark sites of translation, suggesting an intimate connection of mRNA transport and translation. Towards uncovering the code of recognition, we identify UAUG as specific binding motif of Rrm4 that is bound by its third RRM domain. Altogether, we provide first insights into the positional organisation of co-localising RBPs on individual cargo mRNAs.
Where to Live? How Morphology and Evolutionary History Predict Microhabitat Choice by Tropical Tadpoles

Tadpoles have diverse morphologies and occupy diverse habitats. The morphological differences between tadpoles can be represented by linear and geometric measurements and used to explain the organization of tadpole assemblages. However, the effects of evolutionary history must be isolated from the morphological differences before we can determine which patterns result from the use and sharing of common ecological resources. Here, we aimed to determine how morphological similarities and phylogenetic distances affect microhabitat choice by tadpoles. We analyzed the tadpoles of 101 anuran species and classified them according to ecomorphological guild, habitat use, position in the water column, and floor substrate. We used geometric and traditional morphometric approaches to describe the morphological variation among tadpoles and calculated the patristic distance for each species. Afterwards, we used morphometric and phylogenetic matrices as predictors of the variance in the ecological matrix, using a partial redundancy analysis. When we used traditional morphometric data, phylogeny explained a large amount of the ecological variation. By contrast, when we used geometric morphometric data, morphology and phylogeny explained similar amounts of the ecological variation, showing that the technique used to extract morphological variation affects the results. We provide evidence that both morphology, as a surrogate for contemporary factors, and evolutionary inertia are important in determining the behavior of tadpoles. Thus, niche conservatism can be important in modeling the behavior of tadpoles, but does not explain all the preferences of tadpoles.
Gender and Politeness in a Foreign Language Academic Context

Theories and strategies of politeness and impoliteness are drawn upon to investigate underlying factors that might contribute to an understanding of differences among students and teachers towards politeness strategies. Classroom politeness in the present study is operationally defined according to various strategies or behaviors that the students in question draw upon in reacting to different situations. The study explores through a survey and a discourse completion test the degree of politeness the genders in an L1 Arabic context indicate to certain situations. Results show that the possible causes for the 'misunderstandings' and any perceived differences in 'impoliteness' between the genders are more 'cultural' rather than that of 'impoliteness' on the part of the students. Recommendations are made for program coordinators and teachers to deal with this issue in EFL classrooms along with future needed research.
Influence of extreme velocities on dynamic cavity expansion

Cavitation can broadly be described as the unstable expansion of an empty void in a body, usually occurring when loads on the body reach a critical level. In this thesis, dynamic cavity expansion (DCE) in solids is of particular interest. Cavity expansion has been studied extensively under quasi-static and dynamic loading conditions. However, the behavior of cavitation fields with extreme dynamic expansion velocities have little been studied, especially in materials without a definite yield point. In this thesis, DCE in a hardening elastoplastic medium is considered under extreme velocities. Two nonlinear differential equations are used to describe the steady-state expansion. Using numerical integration, this system is solved to explore the behavior under extreme expansion velocities. By gradually increasing the expansion velocities, we find that a singularity occurs in the governing system, indicating a shock wave emerging and propagating through the material. With this limit velocity of the material known, further characteristics of the material can be described and investigated. Thesis Supervisor: Tal Cohen Title: Assistant Professor of Civil and Environmental Engineering
Disagreement over Error Disclosure, Mar 04 ... AMA Journal of Ethics

Mr. Miller continued to have a fever and over the next 24 hours developed low blood pressure and evidence of sepsis, including positive blood cultures. He was transferred to the intensive care unit for management of septic shock. On admission to the ICU, the medication error was noted, and the medication was corrected to cefipime. Mr. Miller required ICU care for 2 days and was on pressors for several hours to maintain his blood pressure. Ultimately he responded to the antibiotics, and he remained in the hospital on antibiotics until his neutropenia resolved.
Effects of Partner Attachment Quality on PTSD Severity with Combat-Exposed Veterans

Posttraumatic stress disorder (PTSD) as a response to a traumatic stressor encompasses re-experiencing, avoidance, and hyperarousal. Although many individuals will experience a traumatic stressor in the course of a lifetime, only a fraction fully develop PTSD. The purpose of this dissertation was to inform the question as to why some develop PTSD as a response to combat exposure and others do not. This study used the PTSD Checklist – Military Version (PCL-M), Trauma Symptom Inventory 2 (TSI-2) and the Attachment and Clinical Issues Questionnaire (ACIQ) to test if secure partner attachments predict PTSD severity, anger, and somatization. These models were not statistically significant. However, exploratory analysis revealed that poorer peer relationships and a withdrawal pattern of social engagement significantly predict PTSD according to the TSI-2 TRAUMA factor score, R 2 = . 41, F (2, 19) = 6.56, p = . 007. These results suggest that better peer relationships may buffer the negative effects of combat exposure. Treatment implications and future research questions are discussed.
Organizational Behavior and Human Decision Processes

Knowledge or idea exchange is an important function of groups in organizations. Much research has demonstrated that idea sharing in groups involves relatively inefficient processes. Some of these processes are briefly summarized. It is proposed that there are conditions under which idea sharing in groups can be productive. One important factor is the extent to which group members carefully process the ideas exchanged in the group (attention). Another is the opportunity for group members to reflect on the ideas after the exchange process (incubation). These factors were examined by using a “brainwriting” paradigm. Evidence was obtained for enhanced performance of sharing groups in comparison to nonsharing or nominal groups both during the idea-generation session and in a second individual idea-generation session. This study suggests that, under the right conditions, the idea exchange process in groups may be an important means for enhancing creativity and innovation in organizations. q 2000 Aca-
Prevalence of Listeria monocytogenes in ready-to-eat foods in Bulgaria.

Listeria monocytogenes is a widespread foodborne pathogen, which is able to grow and survive even at refrigeration temperatures, in vacuum-packed food and in modified atmosphere. It is also often found as an environmental contaminant in food processing plants. Important sources of L. monocytogenes are different ready-to-eat (RTE) products with a long shelf life. RTE are also known as products which support the growth of L. monocytogenes. The microbiological criteria for L. monocytogenes in foodstuffs are specified in Commission Regulation (EC) No 2073/2005. For RTE foods which are able to support the growth of the pathogen, absence in 25 g of the product is required at the end of the production, if the manufacturer is not able to prove that the bacterial counts in the product will not exceed the limit of 100 cfu/g throughout the shelf life. During the period from 01.05. 2011 to 30.04. 2012, in Bulgaria a program for monitoring the prevalence of L. monocytogenes was performed. The monitoring involved three categories of RTE foods gravad and smoked fish, soft and semi-soft cheeses and heat-treated meat products. The monitoring program was a part of the European baseline survey on the prevalence of Listeria monocytogenes in certain ready-to-eat foods at retail in the EU, 2010 2011 (Commission decision 2010/678/ЕС). A total of 367 samples were taken from three categories of RTE foods packaged (not frozen) hot or cold smoked or gravad fish (2 × 122 samples, i.e. duplicates from the same batch), soft and semisoft cheeses (63 samples) and packaged heat-treated meat products (60 samples). The samples were examined qualitatively according to ISO 11290 part 1:1996 and quantitatively according to ISO 11290 part 2:1998. From the fish products duplicate samples were taken and one of the samples was analyzed within 24 h after arrival at the laboratory, whereas the other one was kept refrigerated at the temperature indicated on the label and was examined at the end of the shelf life. The meat products and the cheese samples were tested only at the end of the shelf life. The fish samples were also analyzed for pH (according to ISO 2917:1999) and water activity (according to ISO 21807:2004) immediately after they had been received at the laboratory. L. monocytogenes was detected in 12.3% (15/122) of the fish samples analyzed at the day of receiving and in 8.2% (10/122) of the samples at the end of the shelf life. The highest prevalence of L. monocytogenes was found in cold smoked fish and lower in gravad and hot smoked fish. L. monocytogenes was not detected in any of the meat products and cheese samples. Levels of L. monocytogenes exceeding 100 cfu/g were found in three (2.5%) of the fish samples analyzed at the day of receiving at the laboratory and in one (0.8%) of the samples tested at the end of the shelf-life. The presented data were statistically processed by Student’s t-test. L. monocytogenes was found only in RTE fish products (15 isolates). The maximum level of 100 cfu/g was surpassed in only three of the cases.
Focal mechanism and slip history of the 2011 Mw 9.1 off the Pacific coast of Tohoku Earthquake, constrained with teleseismic body and surface waves

Focal mechanism and slip history of the 2011 Mw 9.1 off the Pacific coast of Tohoku, Japan earthquake were derived rapidly from teleseismic body and surface waves. Multiple double couples (MDC) analysis was first conducted using 1-hour long period seismic waves, yielding a single double couple with a seismic moment of 5.06×1022 N m, whose low angle nodal plane orients 199° and dips 10° west. Spatiotemporal rupture history was then constrained using both broadband body waves and long period seismic waves. The solution was updated twice in three days. Our preferred model, which based on the MDC fault plane, local JMA hypocenter and calibrated alignments of body and surface waves, revealed a complex rupture process. The rupture initiated slowly at a depth of 23 km. It first propagated in downdip and bilaterally directions along the subduction interface for 45 s and then broke a 80 km by 250 km near trench asperity in the up-dip direction and produced up to 60 m slip. The fault plane below the hypocenter had two more subevents, occurring in 70–95 s and 110–160 s, respectively. The total seismic moment was 5.8 × 1022 N m. The abnormal high slip near the trench suggests a recurrence interval larger than 500 yr.
Chapters School of Hotel Administration Collection 1994 Utility Analysis : What are the Black Boxes , and Do They Affect Decisions ?

Excerpt] Utility analysis has been described as 'a family of theories and measures designed to describe, predict, and/or explain what determines the usefulness or desirability of decision options, and to examine how information affects decisions' (Boudreau, 1991, p. 621). Utility analysis (UA) evolved to provide tools for better describing and communicating the impact of HRM and industrial psychology interventions on organizational goals. Most UA research has been based on an implicit assumption that such communication would be aimed toward managers, who control the resources necessary to implement such interventions. Previous research has noted the need to move beyond developing new measures of utility parameters, and to focus on the role of UA information in managerial decision processes (Boudreau, 1989, 1991). Yet there remains a lack of research exploring these issues. In this chapter, we attempt to explicate some of these underlying assumptions and suggest how future UA research may fruitfully test them. We focus specifically on areas where the practice of human resource management seems to diverge from the implied behaviors of UA theory. These deviations provide potential clues about how to enhance the accuracy and usefulness of UA models, and suggest new directions for future UA research and practice. The themes developed in this chapter relate to UA applications in training, compensation, performance assessment, and internal staffing. However, for simplicity and exposition, we will use the external selection model as our guiding framework. External selection is the focus of a significant proportion of UA research, so using this model will relate our themes to a large body of literature. Using the external selection model, this review will attempt to 'open up the black boxes' of four fundamental UA processes: 1. The relationship between predictors and criteria, represented by r, the correlation coefficient. 2. The nature of the criteria, represented by SDy. 3. The nature of the selection process, represented by Zx. 4. The nature of the implementation process, represented by C.
Diffusion-approximation for a kinetic equation with perturbed velocity redistribution process

We derive the hydrodynamic limit of a kinetic equation with a stochastic, short range perturbation of the velocity operator. Under some mixing hypotheses on the stochastic perturbation, we establish a diffusion-approximation result: the limit we obtain is a parabolic stochastic partial differential equation on the macroscopic parameter, the density here.
DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method.

By using the thickness shear mode acoustics method (TSM) and single-molecule force spectroscopy (SMFS) we studied the interactions between DNA aptamers (sgc8c) specific to the protein tyrosine kinase 7 (PTK7), which is localized in the membranes of leukemia lymphoblastics (MOLT-4), and lymphocyte (Jurkat) cell lines, as well with PTK7-negative U266 myeloid leukemia cells. The TSM method allowed the development of a highly sensitive, label-free biosensor for the detection leukemia cells with a limit of detection of (195±20) cells/mL. SMFS approved the high selectivity of the sgc8c aptamers to the PTK7 receptors at the cell surface and allowed determining the binding probability of the aptamers to the PTK7 receptors at different cell lines.
Effect of precipitation fluctuation on soil carbon storage of a tropical peat swamp forest.

Problem statement: It is important to compare the effect of extremely different rainfall conditions on soil carbon storage of lowland tropical peat swamp forest. Therefore, under these natural rainfall gradient, the objectives of this study were to determine whether rainfall affects soil carbon storage of a tropical peat swamp forest and to determine what correlations between variables occurs which stimulate soil carbon storage changes of a tropical peat swamp forest. Approach: Soil sampling was conducted in two different plots (0.3 ha each plot) to a depth of 15 cm under two extremely different mean rainfall at Sibu, Sarawak, Malaysia. The soil samples were analyzed for acidity, organic matter content, total carbon, total nitrogen and total phosphorus. The humic acid extraction was also done and soil carbon storage values were obtained by calculation. The calculation of carbon storage was by the bulk density method. Comparison between paired means of soil carbon storage under two different rainfall gradients were tested using paired t-test and correlation analysis was used to correlate variables (pH, soil organic matter, total carbon, total nitrogen, total phosphorus, C/N ratio, C/P ratio, humic acid yield, unstable carbon and stable carbon). Results: The percentage of stable carbon count of unstable carbon was 42.93% under lower rainfall, while that of higher rainfall was 62.69 %. It suggests that this natural tropical peat swamp forest plays an important role as a sink rather than a source of carbon under higher rainfall but inversely under lower rainfall. It also suggests that soil organic matter tends to decompose and releases CO2 by oxidation under lower rainfall. Stable carbon positively correlated with humic acid yield for the two areas with different rainfall (p<0.01, r = 1.00). However, under higher rainfall, stable carbon also positively correlated with soil organic matter (p<0.05, r = 0.42) and total carbon (p<0.05, r = 0.42). It was found that stable carbon negatively correlated with soil acidity on both higher (p<0.05, r = -0.51) and lower rainfall areas (p<0.01, r = -0.54). However, that association appeared prominent under lower rainfall. Conclusion: Anaerobic environment is more prominent under higher rainfall and may facilitate high value of soil carbon storage in the soil profile of tropical peat swamp forest and allow this ecosystem to function as a carbon sink. During lower rainfall, water availability in tropical peat swamp forest may stimulate this ecosystem to maintain its soil acidity by releasing more CO2 in soil air and becomes a source rather than a sink of carbon.
Heterogeneous Bank Lending Responses to Monetary Policy : New Evidence from a Real-time Identification

We present new evidence on how heterogeneity in banks interacts with monetary policy changes to impact bank lending. Using an exogenous policy measure identified from narratives on FOMC intentions and real-time economic forecasts, we find much greater heterogeneity in U.S. bank lending responses than that found in previous research based on realized federal funds rate changes. Our findings suggest that studies using realized monetary policy changes confound the monetary policy’s effects with those of changes in expected macrofundamentals. We also extend Romer and Romer (2004)’s identification scheme, and expand the time and balance sheet coverage of the U.S. banking sample.
ON ABSOLUTE NORMAL DOUBLE MATRIX SUMMABILITY METHODS

It is shown that, if T is a row nite nonnegative double summability matrix satisfying certain conditions, then jTjk summability is stronger than j N; pnjk summability, for k 1. As special summabil- ity methods T we consider weighted mean and double Ces aro, (C; 1; 1), methods.
MÓNICA ALEXANDRA DOS SANTOS FERREIRA I-2L, UM POSSÍVEL INIBIDOR DA PP1 EM ESPERMATOZÓIDES HUMANOS I-2L, A NOVEL PUTATIVE PP1 INHIBITOR IN HUMAN SPERM

Protein phosphatase-1 (PP1) is a ubiquitously expressed serine/threonine protein phosphatase that controls several cellular processes. In mammalian sperm, changes in PP1 activity correlates with sperm motility development during the transit through the epididymis. The PP1 holoenzyme structure is composed of a conserved subunit termed PP1c and PP1-interacting proteins (PIPs) which direct the holoenzyme to the proximity of its substrates, determining their activity. The catalytic subunit of PP1 is encoded by three different genes termed PP1α, PP1β and PP1γ. PP1γ1 and PP1γ2 are alternatively spliced isoforms of the PP1γ gene. PP1γ1 is ubiquitously expressed whereas PP1γ2 is mainly expressed is testis and sperm. Mammalian inhibitor-2 (I-2) is an ancient PIP that binds to PP1c, resulting in the inhibition of PP1 activity. The reactivation of this complex is triggered by phosphorylation of I-2 at Thr73 residue by GSK-3. Given all the roles that have been proposed to PP1 based on its binding subunits it seemed very important to identify novel PIPs. Thus, in order to search for novel PP1 regulator in human testis yeast two-hybrid screens were performed using PP1γ1 and PP1γ2 as baits to screen a human testis cDNA library. A clone was obtained very similar to I-2, termed I-2 Like (I-2L). After bioinformatics analysis comparison, the main difference detected was Thr73/Pro73 substitution which abolishes the GSK-3 phosphorylation site. This introduces a new regulatory mechanism for the PP1γ2-I-2L complex. The presence of I-2/I-2L in human sperm was confirmed by immunoblot and immunocytochemistry analysis. I-2/I-2L and PP1γ2 co-localize endogenously in the middleand principalpiece of spermatozoa flagellum consistent with the role of PP1γ2-(I-2/I-2L) complex in sperm motility. The similarities between I2/I-2L in terms of amino acid sequences (95%) introduced a challenge to differentiate I-2 and I-2L proteins. Hence, the proteolytic fingerprint of I-2 and I2L was analyzed for further mass spectrometry (MS) analysis, and concluded that this was a fine approach to prove the existence of I-2L in vivo. Studies are now in progress in order to validate, via MS analysis, the amino acid sequence of I-2L immunoprecipitated from human sperm. Inhibitor-2L, a novel putative protein phosphatase-1 inhibitor in human sperm Mestrado em Biologia Molecular e Celular 7 Abbreviations . 8
Measuring two‐dimensional movements using a single InSAR pair

We present a new method to extract along‐track displacements from InSAR data, based on split‐beam InSAR processing, to create forward‐ and backward‐looking interferograms. The phase difference between the two modified interferograms provides the along‐track displacement component. Thus, from each conventional InSAR pair we extract two components of the displacement vector: one along the line of sight, the other in the along‐track direction. We analyze the precision of the new method by comparing our solution to GPS and offset‐derived along‐track displacements in interferograms of the 1999, Hector Mine earthquake. The RMS error between GPS displacements and our results ranges from 5 to 8.8cm. Our method is consistent with along‐track displacements derived by pixel‐offsets, which are limited to 12–15cm precision. The theoretical precision of the new method depends on SNR and coherence. For a signal to noise ratio of 30 the expected precisions are 3, 11cm for coherence of 0.8, 0.4, respectively.