Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular analysis of R-factors from multiresistant nosocomial isolates.

During an epidemic of infections at the Seattle Veterans Hospital, Washington, due to a multiresistant strain of Serratia marcencens, other enteric species were isolated that had antibiograms nearly identical to those of the epidemic S. marcescens. In 11 instances, these multiresistant species were isolated from specimens that also contained the epidemic serratia strain. All isolates of the epidemic serratia strain contained a conjugative 45-megadalton R-factor (pLST1000) coding for intermediate resistance to three amino-glycosides (minimal inhibitory concentrations, 4--8 micrograms/ml) and high-level resistance to ampicillin, carbenicillin, cephalothin, streptomycin, and sulfonamide. With the use of agarose gel electrophoresis and restriction endonuclease cleavage patterns after digestion with EcoRI, BamH1, and HindIII, it was determined that eight different enteric strains of six different species isolated from the patients contained an R-factor that was molecularly identical to the one isolated from the epidemic strain of S. marcescens. Thus, the epidemic of multiresistant infections at this hospital was caused both by the spread of an epidemic strain and an "epidemic plasmid. " The molecular characteristics of pLST1000 appear to be different from previously described multiresistant plasmids.
Exploring Implementation of m-Health Monitoring in Postpartum Women with Hypertension.

BACKGROUND Preeclampsia is a hypertensive disorder in pregnancy where a patients' blood pressure and warning signs of worsening disease need to be closely monitored during pregnancy and the postpartum period. INTRODUCTION No studies have examined remote patient monitoring using mobile health (m-health) technologies in obstetrical care for women with preeclampsia during the postpartum period. Remote monitoring and m-health technologies can expand healthcare coverage to the patient's home. This may be especially beneficial to patients with chronic conditions who live far from a healthcare facility. MATERIALS AND METHODS The study was designed to identify and examine the potential factors that influenced use of m-health technology and adherence to monitoring symptoms related to preeclampsia in postpartum women. A sample of 50 women enrolled into the study. Two participants were excluded, leaving a total sample size of 48 women. Users were given m-health devices to monitor blood pressure, weight, pulse, and oxygen saturation over a 2-week period. Nonusers did not receive equipment. The nurse call center monitored device readings and contacted participants as needed. Both groups completed a baseline and follow-up survey. RESULTS Women who elected to use the m-health technology on average had lower levels of perceived technology barriers, higher facilitating condition scores, and higher levels of perceived benefits of the technology compared with nonusers. Additionally, among users, there was no statistical difference between full and partial users at follow-up related to perceived ease of use, perceived satisfaction, or perceived benefits. DISCUSSION This study provided a basis for restructuring the management of care for postpartum women with hypertensive disorders through the use of m-health technology. CONCLUSION Mobile health technology may be beneficial during pregnancy and the postpartum period for women with preeclampsia to closely manage and monitor their blood pressure and warning signs of worsening disease.
Judging the Truth-Value of a Statement In and Out of a Deep Processing Context

Individuals judge fluently processed statements truer than disfluent statements, which reflects an illusion of truth. A dual-processing approach to the truth effect suggests that cognitive resources and motivation for accuracy should moderate this effect. However, previous research has only manipulated participants’ capacity during the encoding of the statements. We directly test an account of the truth effect based on processing features of System 1, manipulating both capacity and motivation at the time of the truth judgments. Fluency was manipulated either through repetition (Experiment 1) or perceptual contrast (Experiment 2). Congruent with dualistic assumptions, the evaluations of participants in the high-capacity and high-motivation conditions differed from those of participants in the other three conditions. However, the pattern of the truth effect in that specific condition was not the same in both experiments: in Experiment 1, the illusion of truth was only reduced, whereas the effect was complet.
Stereotyped subset #1 chronic lymphocytic leukemia: a direct link between B‐cell receptor structure, function, and patients' prognosis

Chronic lymphocytic leukemia (CLL) with stereotyped B‐cell receptor (BCR) belonging to subset #1 (IGHV1‐5‐7/IGKV1‐39) display a poor outcome. To characterize their genetic and genomic features and BCR function, we selected 20 subset #1 CLL from a series of 579 cases. Subset #1 CLL, all showing unmutated IGHV, were associated with the presence of del(11q) (50%) in comparison with unmutated CLL, unmutated stereotyped CLL other than subset #1 and with cases using the same IGHV genes but a heterogeneous VH CDR3 (non‐subset #1 CLL). There were no distinctive features regarding CD38, ZAP‐70, and TP53 disruption. NOTCH1, SF3B1, and BIRC3 were mutated in 15%, 0%, and 5% of cases, respectively, while BIRC3 was deleted in 22% of cases. Microarray unsupervised analysis on 80 unmutated/mutated/stereotyped/non‐stereotyped CLL showed a tight clustering of subset #1 cases. Their genomic signature exhibited several differentially expressed transcripts involved in BCR signal transduction, apoptosis regulation, cell proliferation, and oxidative processes, regardless of del(11q). Accordingly, BCR ligation with anti‐IgM revealed a significant higher proliferation of subset #1 versus unmutated non‐subset #1 CLL, both at baseline and after 24–48 hr stimulation. Subset #1 CLL represent a paradigmatic example of the direct link between BCR structure, function, and patients prognosis. Am. J. Hematol. 89:74–82, 2014. © 2013 Wiley Periodicals, Inc.
Global Convergence of a Nonsmooth Newton Method for Control-State Constrained Optimal Control Problems

We investigate a nonsmooth Newton method for the numerical solution of optimal control problems subject to mixed control-state constraints. The necessary conditions are stated in terms of a local minimum principle. By use of the Fischer-Burmeister function the local minimum principle is transformed into an equivalent nonlinear and nonsmooth equation in appropriate Banach spaces. This nonlinear and nonsmooth equation is solved by a nonsmooth Newton's method. We prove the global convergence and the locally superlinear convergence under certain regularity conditions. The globalized method is based on the minimization of the squared residual norm. Numerical examples for the Rayleigh problem conclude the article.
The Oligomeric Outer Dynein Arm Assembly Factor CCDC103 Is Tightly Integrated within the Ciliary Axoneme and Exhibits Periodic Binding to Microtubules*

Background: CCDC103 is necessary for the assembly of outer dynein arms on ciliary microtubules. Results: CCDC103 is arrayed along the axoneme, self-assembles, and binds microtubules with a 12-nm periodicity. Conclusion: CCDC103 is an integral component of the axoneme that underlies dynein assembly. Significance: Defining how dynein arms are incorporated is necessary to understand the complex patterning of motile cilia. CCDC103 is an ∼29-kDa protein consisting of a central RPAP3_C domain flanked by N- and C-terminal coiled coils. Defects in CCDC103 lead to primary ciliary dyskinesia caused by the loss of outer dynein arms. This protein is present along the entire length of the ciliary axoneme and does not require other dynein or docking complex components for its integration. Unlike other known dynein assembly factors within the axoneme, CCDC103 is not solubilized by 0.6 m NaCl and requires more chaotropic conditions, such as 0.5 m KI. Alternatively, it can be extracted using 0.3% sarkosyl. CCDC103 forms stable dimers and other oligomers in solution through interactions involving the central domain. The smallest particle observed by dynamic light scattering has a hydrodynamic diameter of ∼25 nm. Furthermore, CCDC103 binds microtubules directly, forming ∼9-nm diameter particles that exhibit a 12-nm spacing on the microtubule lattice, suggesting that there may be two CCDC103 units per outer arm dynein repeat. Although the outer dynein arm docking complex is necessary to form arrays of dyneins along microtubules, it is not sufficient to set up a single array in a precise location on each axonemal doublet. We propose that CCDC103 helps generate a high-affinity site on the doublets for outer arm assembly, either through direct interactions or indirectly, perhaps by modifying the underlying microtubule lattice.
Analyzing the WISC-R In Children with ADHD: The Predictive Value of Subtests, Kaufman, and Bannatyne Categories 2

SUMMARY Objective: The aim of this study was to evaluate the predictive value of Intelligence Quotient scores (IQs), subtests of Wechsler Intelligence Scale for Children-Revised (WISC-R), and the Kaufman’s and Bannatyne’s categories scores in attention deficit hyperactivity disorder (ADHD). In addition, this study was designed to examine the difference of some neurocognitive skills for children with ADHD and their unaffected peers using the WISC-R subtests. Method: WISC-R’s subtest and IQ scores, and scores of Kaufman’s and Bannatyne’s categories of the children who were diagnosed with only ADHD were compared with the same scores of the children in the healthy control group (N= 111) and the ADHD with comorbid group (N= 82). Results: It was found that the subtest scores (vocabulary, comprehension, digit span, picture completion, and block design) of the children with only ADHD and ADHD with comorbidity were significantly lower than the healthy group. In addition, object assembly subtest scores of the children with only ADHD were lower than the control group. Subtests of comprehension (Wald = 5.47, df = 1, p=0.05), digit span (Wald = 16.79, df = 1, p=0.001), and picture completion (Wald= 5.25, df= 1, p=0.05) were able to predict ADHD significantly. In this study, the categories of freedom from distractibility (Wald = 8.22, df = 1, p=0.01) and spatial abilities (Wald = 12.22, df = 1, p<0.0001) were also predictive for ADHD. Conclusion/Discussion: Problem-solving abilities in social processes, auditory short-term memories, visual-spatial abilities, and visual configuration abilities of the children with ADHD were lower than their healthy peers. It was thought that in WISC-R’s profile analysis, the categories of freedom from distractibility and spatial abilities could be distinctive in ADHD diagnosis.
Light-Reflection Liquid-Level Sensor

When parallel-polarized light is reflected at an angle ¿ by the interface between two transparent media with refractive indices n and N, the interface reflectance is switched from 1 to 0 by varying the refractive index ratio N/n from sin ¿ to tan ¿. This is the basis of a light-reflection liquid-level sensor that uses a prismatic window in the side wall of the vessel whose liquid level is to be monitored. Such a sensor is described and its design procedure is developed. Remote liquid-level telemetering is possible by using fiber optics to lead the light to and from this particular sensor.
On the Radial Density Profile of Intracluster Gas Tracing the Isothermal Dark Halo with a Finite Core

The cusped Navarro, Frenk, & White (NFW) universal density profile suggested by typical cold dark matter (CDM) models has been challenged in recent years by the discovery of soft cores with finite central density for a broad range of masses from dwarf galaxies to clusters of galaxies. It is thus desirable that a new, analytic model should instead become available for virialized dark halos. One promising candidate is probably the empirical density profile proposed by Burkert, which resembles an isothermal profile with a constant core in the inner region and matches the NFW profile at large radii. Meanwhile, such a revised dark halo (RDH) profile has turned out to be a great success on galactic scales. This stimulates us to apply the RDH profile to more massive systems such as clusters of galaxies. In this paper we have made an attempt to derive the radial density profile of intracluster gas from the RDH profile under the isothermal and hydrostatic equilibrium hypotheses, and compare it with those revealed by X-ray observations and inferred from the NFW profile. We show that the RDH-predicted gas density can be well represented by the conventional β model with a typical β parameter of β ~ 0.7-0.9. Alternatively, fitting the theoretically predicted X-ray surface brightness profile to an ensemble of 45 X-ray clusters observed by ROSAT, we find that the RDH and NFW profiles become almost indistinguishable from each other, and their characteristic density and scale length parameters are strongly correlated. Yet, unlike the NFW model, the RDH profile can allow us to work out straightforwardly the central dark matter density from X-ray measurements of the surface brightness and temperature of clusters. It appears that the resulting central densities of the 45 clusters have an average value of ⟨ρ0⟩ ≈ 0.01 M☉ pc-3, in agreement with the result estimated on galactic scales, which reinforces the claim for the presence of soft halo cores over the entire mass range.
Adverse drug reactions—a matter of opinion

The accurate identification of adverse drug reactions (ADRs) is difficult because ADRs usually present no unique clinical or laboratory findings that demarcate them from the manifestations of concurrent illnesses. The identification of ADRs depends on the clinical assessments of physicians‐sometimes the clinician treating the patient and at other times a clinical pharmacologist. Considering the complex and subjective nature of clinically identifying ADRs, how accurately are ADRs identified? To answer this question, three clinical pharmacologists each independently evaluated 60 selected cases to determine if medication, alcohol, or “recreational” drugs had caused the hospitalization. The three clinical pharmacologists agreed on only 30 cases (50%), and 27 of these were thought to be unrelated to medications. In 19 of the 30 cases about which the clinical pharmacologists disagreed, they disagreed on whether or not a medication‐ or alcohol‐related event had occurred at all. The clinical pharmacologists disagreed with the physicians treating the patient in 22% to 37% of the cases, but because of the differences among the pharmacologists, the treating physicians agreed with at least one of them in 95% of the cases. Complete agreement between the clinical pharmacologists and the treating physicians occurred in 47% of the cases. This degree of disparity in the clinical identification of ADRs shows that the evaluation of ADRs is subjective and imprecise. The accurate identification of ADRs awaits the development of an objective technique for recognizing ADRs.
The Osmoregulatory Ability of the Lampern (Lampetra Fluviatilis L.) In Sea Water During the Course of its Spawning Migration

1. Although fresh-run lamperns are able to withstand the effects of increasing sea-water concentration better than maturing animals, they can only maintain their plasma chloride constant in environments more dilute than 50% sea water. This is achieved, in part, by gradually reducing the urine output from the normal fresh-water level (155.8 ml./kg./day) to a negligible rate in solutions which are mildly hypertonic to the blood (33% sea water). 2. Studies on the rate of change of weight loss, of plasma chloride and of plasma osmotic pressure following abrupt immersion in dilute sea water show that mature lamperns cannot osmoregulate and can only survive in 33% sea water by tolerating a raised blood osmotic pressure caused by water loss. 3. Similar experiments on fresh-run animals suggest that the external surfaces of their bodies are less permeable to water than mature animals. Unlike mature animals, they also show considerable variation in the way in which they respond to 33% sea water. Some are able to maintain their plasma osmotic pressure and chloride well below that of the environment. These animals also show little loss in weight, and this indicates that water is taken up actively, since this process has been shown to occur in some animals. Other fresh-run animals show raised plasma osmotic pressures in varying degrees and these are associated with larger losses of weight. These facts suggest that the hypotonic regulating mechanism gradually degenerates as the lampern enters fresh water.
Fear and anger regulation in infancy: effects on the temporal dynamics of affective expression.

Emotion regulation has been conceptualized as the extrinsic and intrinsic processes responsible for monitoring, facilitating, and inhibiting heightened levels of positive and negative affect. Regulation of distress is related to the use of certain behavioral strategies. Our study examined whether putative regulatory behaviors widely assumed to be conceptually associated with these strategies are actually empirically associated with the changes in fearful and angry distress in 6-, 12-, and 18-month-old infants. Our key finding was that the use of some putative regulatory behaviors (e.g., distraction and approach) reduced the observable intensity of anger but were less effective in reducing the intensity of fear. The results suggest (1) caution in assuming that postulated regulatory behaviors actually have general distress-reducing effects and (2) the likelihood that "distress" is too global a construct for research on emotion regulation.
FETAL MOVEMENT PATTERNS IN THE FIRST TRIMESTER OF PREGNANCY

Fetal movements were examined at various ages during the first trimester of pregnancy in 46 patients. Characteristic types and patterns of movement were found to relate to the age of the fetus. By 10 to 12 weeks, a full range of movements had developed and the movement patterns then closely resembled those normally found in the second and third trimesters of normal pregnancy.
Inflammatory Bowel Disease in the United States from 1998 to 2005: Has Infliximab Affected Surgical Rates?

The treatment costs for patients in the United States with inflammatory bowel disease (IBD) exceed 1.7 billion dollars/year. Infliximab, an antibody to tumor necrosis factor-α, has been extensively used to treat IBD, with 390,000 IBD patients receiving the drug since its FDA approval in 1998. We sought to determine the impact of infliximab on population-based rates of hospitalizations and surgical care for patients with IBD in the United States. We used data from the Nationwide Inpatient Sample to analyze patterns of hospital-based treatment provided to patients with IBD between 1998 and 2005. Data from this analysis were combined with census data to calculate trends in population-based rates of treatment. Overall rates of hospitalization for patients with Crohn's disease and ulcerative colitis increased significantly between 1998 and 2005 (5.1%/year and 3.4%/year respectively, P < 0.001 for each). During the same time period there were no changes in the overall rates of surgical care. The expanding use of infliximab has not significantly impacted the use of surgical procedures for patients with either ulcerative colitis or Crohn's disease, and rates of nonsurgical hospitalizations have actually increased. Even in the era of infliximab, surgical care remains a mainstay in the treatment of IBD.
Risk of Intravesical Recurrence After Ureteroscopic Biopsy for Upper Tract Urothelial Carcinoma: Does the Location Matter?

INTRODUCTION To investigate whether ureteroscopic biopsy (URS-Bx) for upper tract urothelial carcinoma (UTUC) before radical nephroureterectomy affects intravesical recurrence (IVR). PATIENTS AND METHODS Of the 515 patients receiving radical nephroureterectomy for UTUC between 1998 and 2012, 387 patients were included for the analysis. URS-Bx was performed in 69 patients (17.8%). Patients were stratified according to tumor location, and the impact of URS-Bx on IVR was evaluated after adjusting for other clinicopathologic variables. RESULTS IVR occurred in 163 patients (42.1%). The postoperative 5-year IVR-free survival was not significantly different according to URS-Bx in the overall patient group (54.0% vs 39.3%, p = 0.056). In patients with renal pelvic tumor, IVR-free probability was lower in patients with URS-Bx than in patients without URS-Bx (39.6% vs 62.2%, p = 0.012), although IVR-free survival in patients with ureteral tumor was equivalent (36.7% vs 45.8%, p = 0.946). In multivariate analysis, previous bladder tumors (hazard ratio [HR], 1.65; p = 0.016) were a significant risk factor for IVR in all patients and ureteral tumor location (HR, 1.35; p = 0.071) was associated with IVR, although statistical significance was not achieved. In patients with renal pelvic tumor, URS-Bx HR, 1.98; p = 0.020 was the only risk factor for IVR. However, URS-Bx was not associated with IVR in patients with ureteral tumor, although previous bladder tumor HR, 1.74; p = 0.028 was a risk factor for IVR. CONCLUSION URS-Bx is a significant risk factor for IVR in patients with renal pelvic tumor and should be performed after sufficient consideration in these patients if other examinations are uncertain.
Antibacterial properties and reduction of MRSA biofilm with a dressing combining polyabsorbent fibres and a silver matrix.

OBJECTIVE This study was designed to evaluate the antibacterial activity of a wound dressing which combines polyacrylate fibres and a silver lipido-colloid matrix (UrgoClean Ag, silver polyabsorbent dressing), against biofilm of methicillin-resistant Staphylococcus aureus (MRSA). METHOD Samples of silver polyabsorbent dressing and the neutral form of this dressing (UrgoClean) were applied to biofilms of MRSA formed on a collagen I-coated surface, cultured for 24 hours. Different exposure times were tested (1, 2, 4 and 7 days) without dressing change. The biofilm reduction was quantified by using culture methods and by confocal laser scanning microscopy experiments. RESULTS The application of the silver polyabsorbent dressing resulted in a significant decrease of the biofilm population by a log reduction of 4.6, after 24 hours of exposure. Moreover, the antibiofilm activity was maintained for 7 days with reduction values up to 4 log (reduction of biofilm superior to 99.99%). The application of the neutral dressing also induced a significant reduction of the concentration of sessile cells after 1 day (about 0.90 log). The results obtained with this neutral form of the dressing showed that the polyacrylate fibres were able to exert a mechanical disruption of the biofilm architecture. CONCLUSION These in vitro experiments demonstrated that silver polyabsorbent dressing was able to strongly reduce the biofilm of MRSA. The antibiofilm mechanism of this dressing can be explained by a dual action of the polyabsorbent fibres (based on ammonium polyacrylate polymer around an acrylic core) which induced a mechanical disruption of the biofilm matrix and/or a sequestration of sessile cells, and the diffusion of silver ions which produced bactericidal activity. DECLARATION OF INTEREST This study was supported by Laboratoires Urgo (Dijon). P. Janod is an employee of Laboratoires Urgo. The company had no influence on the experimental design and the interpretation of the results.