Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic diversity, taxonomy and legumins implications of seed storage protein profiling in Fabaceae

Proteomic evidences can be pivotal to the discovery of new plant proteins and plant relationships, due to the diversity of form it can reveal. Seed storage protein profiles of 20 Fabaceae species: 4 grainlegumes and 16 non-pulses; of 16 genera and 10 tribes were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to estimate protein content diversity and the possible genetic relatedness. 28.3% similarity and 71.7% proteomic polymorphism was scored for the species. The high variability expressed by the lot reflects the genetic diversity amongst Fabaceae population. Dendrogram based on the proteomic data clustered the species into four groups. Aside two species, Albizia lebbeck and Albizia zygia belonging to the tribe Ingeae and those of the tribe Caesalpinieae , the other species clustered with several other non-traditional cohorts resulting in a rearrangement that showed least semblance with phylogenetic relationships based on traditional morphology taxonomic delimitation. The similarity in profiles can be preliminarily forensic for proteins of importance whether for nutritional, industrial or for improvement of existing crops or for entirely new plants as crops. The protein mix, and the resultant relationship based on seed storage proteins instigates a review of erstwhile taxonomic, agricultural and research perspectives for the Fabaceae. Keywords : Fabaceae, seed protein, polymorphism, genetic diversity, taxonomy, single protein African Journal of Biotechnology Vol. 12(17), pp. 2157-2163
Non-failable approximation method for conditioned distributions

We consider a general method for the approximation of the distribution of a process conditioned to not hit a given set. Existing methods are based on particle system that are failable, in the sense that, in many situations , they are not well defined after a given random time. We present a method based on a new particle system which is always well define. Moreover , we provide sufficient conditions ensuring that the particle method converges uniformly in time. We also show that this method provides an approximation method for the quasi-stationary distribution of Markov processes. Our results are illustrated by their application to a neutron transport model.
Nodular Goiter and Thyroid Carcinoma in Children and Adolescents in a Moderate Endemic Area (Lower Silesia-Sudeten Endemia) in the Last Twelve Years

Although thyroid carcinoma is more common in the adult population, the risk of a nodule being malignant is greater in children. The aim of our present investigation was to ascertain the percentage of malignancy in nodular goiter observed in patients from the Lower Silesia region in the last 12 years. The examination included 60 children (12 boys and 48 girls) treated in our clinic from 1987 to June 1998. Age varied from 7 to 18 years (mean 14.8 +/- 2.4), most of them in the age group between 13 and 18 years. The following investigations were performed: TSH, T3, T4, thyroid ultrasonography, fine needle aspiration biopsy and Tc99 scintigraphy of the thyroid. Most of the patients were euthyroid; two children demonstrated pressure symptoms. All the patients were treated by operation. Histological examinations revealed the following: nodular goiter in 19 patients, cystic nodular goiter in 5, follicular adenoma in 20, fetal adenoma in 3, nodular goiter and follicular adenoma in 6, papillary carcinoma in 6, and follicular carcinoma in 1 patient. We concluded that an increased incidence of thyroid cancer has been noted in children with nodular goiter in Lower Silesia during the last 12 years. Thyroid cancer was observed mostly in patients with single nodules and was associated with a high risk of malignancy.
The role of endothelial cell Ca2+ store release in the regulation of microvascular permeability in vivo

Microvascular permeability is regulated by changes in intracellular calcium concentration. The mechanism by which this increase in calcium determines permeability under normal conditions and during stimulation with agonists remains to be elucidated. In order to determine whether calcium release from intracellular stores could contribute towards the regulation of vascular permeability, hydraulic conductivity (Lp) was measured in frog mesenteric microvessels during stimulation of the endothelial cells of these vessels with agonists that release calcium from the intracellular stores. ATP (which acts through activation of inositol 1,4,5‐trisphosphate (IP3) receptors) increased Lp in the absence of calcium influx across the plasma membrane 2.3 ± 0.3 fold (mean ±s.e.m., P < 0.01, n= 8), which was less than the increase in the presence of calcium influx (3.1 ± 1.1 fold). Caffeine (which acts through activation of ryanodine receptors) also increased Lp in the absence of calcium influx across the plasma membrane 3.8 ± 1.0 fold (P < 0.01, n= 9), but by at least as much as it does in the presence of calcium influx (2.8 ± 0.5 fold). It is surprising that there was a strong positive correlation between the size of the response during store release and the baseline permeability (r = 0.91 for ATP, r = 0.75 for caffeine). This suggests that the filling state of the stores may regulate the baseline permeability of the microvessels.
Stability and Variability of the Thermohaline Circulation in the Past and Future: a Study with a Coupled Model of Intermediate Complexity

We present the results of a study of the stability properties of the thermohaline ocean circulation (THC) and the possible mechanisms of rapid climate changes using the climate system model of intermediate complexity, CLIMBER-2. We consider two climate states, the warm modern climate and the cold glacial climate, and compare the stability properties of the THC in both cases. While for modern climate there are two stable circulation modes: the warm and the off mode, under glacial conditions we find only one (cold) stable mode of Atlantic ocean circulation. This mode is characterized by deep water formation south of Iceland and has a shallower overturning with weaker outflow to the southern Atlantic. Another mode of the THC similar to modern warm mode is marginally convectively unstable under glacial conditions and a relatively small negative perturbation in the freshwater flux to the Nordic Seas can trigger rapid transitions between cold and warm modes. After being excited, the warm mode is metastable on the time scale of a few hundred years. We speculate that the stable cold mode corresponds to stadial periods with cold climate over the Northern Atlantic, while temporal transitions between cold and warm modes could explain the observed Dansgaard-Oeschger oscillations. The warm modern climate does not possess this type of instability, but rapid global warming can trigger a complete collapse of the THC. We discuss the mechanisms and uncertainties of future changes in the THC.
Quality of Life, Needs, and Interest Among Cocaine Users: Differences by Cocaine Use Intensity and Lifetime Severity of Addiction to Cocaine

We examined the quality of life (QoL) of 149 patients who were recruited in 2005 at outpatient treatment centers for cocaine dependence in Spain. Important life areas and life areas with potential need and interest to change in order to improve the QoL were analyzed in terms of patients’ cocaine use intensity within the previous six months and lifetime severity addiction to cocaine. The Spanish versions of the Drug User Quality of Life Scale and the Lifetime Severity Index for Cocaine were used to measure QoL, needs and interest, and severity addiction to cocaine. The data analysis employed t-tests, linear regression, Mann–Whitney U tests, multivariate regression, and chi-square tests. Tailoring treatment programs to address the life areas that are considered relevant to cocaine users considering their intensity of consumption and lifetime severity addiction to cocaine may improve retention and treatment outcomes. Further research needs to consider patients of different ethnic backgrounds and cultural contexts. The study's limitations are noted.
MACROLIDES PROTECT AGAINST PSEUDOMONAS AERUGINOSA INFECTION VIA INHIBITION OF INFLAMMASOMES

Macrolides antibiotics have been effectively used in many chronic diseases, especially with Pseudomonas aeruginosa (P. aeruginosa) infection. The mechanisms underlying the therapeutic effects of macrolides in these diseases remain poorly understood. We established a mouse model of chronic lung infection using P. aeruginosa agar-beads, with azithromycin treatment or placebo. Lung injury, bacterial clearance, and inflammasome-related proteins were measured. In vitro, the inflammasomes activation induced by flagellin or ATP were assessed in LPS-primed macrophages with or without macrolides treatment. Plasma IL-18 levels were determined from patients who were diagnosed with bronchiectasis isolated with or without P. aeruginosa and treated with azithromycin for 3-5 days. Azithromycin treatment enhanced bacterial clearance and attenuated lung injury in mice chronically infected with P. aeruginosa, which resulted from the inhibition of caspase-1-dependent IL-1β and IL-18 secretion. In vitro, azithromycin and erythromycin inhibited NLRC4 and NLRP3 inflammasomes activation. Plasma IL-18 levels were higher in bronchiectasis patients with P. aeruginosa isolation compared with healthy controls. Azithromycin administration markedly decreased IL-18 secretion in bronchiectasis patients. The results of this study reveal that azithromycin and erythromycin exert a novel anti-inflammatory effect by attenuating inflammasomes activation, which suggests potential treatment options for inflammasome-related diseases.
Effect of Anterior Cruciate Ligament Reconstruction and Meniscectomy on Length of Career in National Football League Athletes

Background Meniscal and anterior cruciate ligament (ACL) injuries are common in college football athletes. The effect of meniscectomy and/or ACL surgery on the length of an athlete’s career in the National Football League (NFL) has not been well examined. Hypothesis Athletes with a history of meniscectomy or ACL surgery before the NFL combine have a shorter career than matched controls. Study Design Case-control study; Level of evidence, 3. Methods A database containing the injury history and career NFL statistics of athletes from 1987–2000 was used to match athletes with a history of meniscectomy and/or ACL surgery, and no other surgery or major injury, to controls without previous surgeries. Athletes were matched by position, year drafted, round drafted, and additional injury history. Results Fifty-four athletes with a history of meniscectomy, 29 with a history of ACL reconstruction, and 11 with a history of both were identified and matched with controls. Isolated meniscectomy reduced the length of career in years (5.6 vs 7.0; P = .03) and games played (62 vs 85; P = .02). Isolated ACL surgery did not significantly reduce the length of career in years or games played. Comparing the athletes with meniscectomy or ACL reconstruction to athletes with combined ACL reconstruction and meniscectomy, a history of both surgeries, resulted in a shorter career in games started (7.9 vs 35.1; P <.01), games played (41 vs 63; P = .07), and years (4.0 vs 5.8; P = .08) than a history of either surgery alone. Conclusion A history of meniscectomy, but not ACL reconstruction, shortens the expected career of a professional football player. A combination of ACL reconstruction and meniscectomy may be more detrimental to an athlete’s durability than either surgery alone. Further research is warranted to better understand how these injuries and surgeries affect an athlete’s career and what can be done to improve the long-term outcome after treatment.
The new challenge: mobile multimedia sensor networks

Along with the fast development of sensor hardware, traditional scalar sensor nodes are now provided with both multimedia and mobility functions. By observing this emerging combination of these two functions into traditional wireless sensor networks (WSNs), we take the opportunity in this paper to highlight a subset of WSNs, which we name as ‘mobile multimedia sensor networks’ (MMSNs). Furthermore, we present a number of challenging issues that may appear in MMSNs in terms of enhancing diverse quality of services (QoS) and prolonging the network lifetime for both WSNs and multimedia communication research communities.
Effects of Hemodynamic Differences on the Assessment of Inter-Brain Synchrony Between Adults and Infants

The simultaneous recording of brain activity in two or more people, termed hyperscanning, is an emerging field of research investigating the neural basis of social interaction. Hyperscanning studies of adult–infant dyads (e.g., parent and infant) have great potential to provide insights into how social functions develop. In particular, taking advantage of functional near-infrared spectroscopy (fNIRS) for its spatial resolution and invulnerability to motion artifacts, adult–infant fNIRS may play a major role in this field. However, there remains a problem in analyzing hyperscanning data between adult and young populations. Namely, there are intrinsic differences in hemodynamic time latencies depending on age, and the peak latency of the hemodynamic response function (HRF) is longer in younger populations. Despite this fact, the effects of such differences on quantified synchrony have not yet been examined. Consequently, the present study investigated the influence of intrinsic hemodynamic differences on wavelet coherence for assessing brain synchrony, and further examined the statistical removal of these effects through simulation experiments. First, we assumed a social signal model, where one counterpart of the dyad (e.g., infant) sends a social signal to the other (e.g., parent), which eventually results in simultaneous brain activation. Based on this model, simulated fNIRS activation sequences were synthesized by convolving boxcar event sequences with HRFs. We set two conditions for the event: synchronized and asynchronized event conditions. We also modeled the HRFs of adults and infants by referring to previous studies. After preprocessing with additional statistical processing, we calculated the wavelet coherence for each synthesized fNIRS activation sequence pair. The simulation results showed that the wavelet coherence in the synchronized event condition was attenuated for the combination of different HRFs. We also confirmed that prewhitening via an autoregressive filter could recover the attenuation of wavelet coherence in the 0.03–0.1 Hz frequency band, which was regarded as being associated with synchronous neural activity. Our results showed that variability in hemodynamics affected the analysis of inter-brain synchrony, and that the application of prewhitening is critical for such evaluations between adult and young populations.
Review: Endothelial progenitor cells: markers of vascular reparative capacity

Assessment of the propensity for vascular events has been based on measurement of risk factors predisposing one to vascular injury. These assessments are based on the strong associations between risk factors such as hypertension, cholesterol levels, smoking, and diabetes which were first described almost a half century ago. The more recent discovery of the relationship between ongoing inflammation and clinical outcomes has led to a variety of blood-based assays which may impart additional knowledge about an individual's propensity for future cardiovascular events. Vascular health is now better represented as a balance between ongoing injury and resultant vascular repair, mediated at least in part by circulating endothelial progenitor cells. To date, one's risk for vascular events has focused exclusively on assessing propensity for vascular damage, either by assessing conventional risk factors which were initially identified over half a century ago, or more recently by assessing markers of inflammation and other circulating factors which area related to subsequent clinical events. Circulating endothelial progenitor cells play important roles in accelerating endothelialization at areas of vascular damage, and EPC enumeration is a viable strategy for assessing reparative capacity. To date, EPC numbers have been correlated with the numbers of cardiovascular risk factors, extent of coronary disease, and future cardiovascular events. Given that EPC enumeration and functional characterization represent the only assessment of the reparative side of the balance between damage and renovation, this technique may offer independent and different assessment of propensity to cardiovascular injury, greatly improving risk stratification of patients.
Changes in bone mineral content with decreased training in competitive young adult tennis players and controls: a prospective 4-yr follow-up.

PURPOSE The purpose of this 4-yr prospective follow-up study of male tennis players was to determine the changes in the playing-to-nonplaying arm differences in bone mineral content (BMC) and answer two questions: 1) Are training induced bone gains lost with decreased training? and 2) Are the possible bone changes related to the changes in training? METHODS Subjects were 13 former competitive male tennis players and their 13 controls. The players had started their playing career at the mean age of 11 yr. The initial comparisons between the playing and contralateral arms' BMC were done in 1992 (mean age 26 yr), and follow-up was performed 4 yr later. The players were competing at the national top level in the beginning of the study, and all had retired from the top tennis (mean) 2.3+/-0.6 yr before follow-up. Accordingly, they had reduced the average training frequency from 5.2 times a week to 2.6 times a week and the mean hours of training per week from 7.6 to 3.3. RESULTS The 1996 measurements revealed that on average the players' exercise-induced bone gain in the playing arm had not disappeared with time despite decreased average playing activity and that observed bone changes were not related to changes in training. The side-to-side BMC differences were largest in the humeral shaft (25% in 1992 and 26% 4 yr later) and the proximal humerus (19% and 18%). The radial shaft difference was 14% and the distal radius difference 13% in both 1992 and 1996. In controls, the side-to-side BMC differences were small, less than 5%, and they did not change by time. CONCLUSIONS In conclusion, this study suggests that bone gain obtained by regular tennis training does not disappear by time despite decreased playing activity. Regular exercise can be valuable for maximizing peak bone mass and thus contributing to prevention of osteoporosis and related fractures later in life.
Vessel- and target cell-specific actions of endothelin-1 and endothelin-3 in rat liver.

We studied the sinusoidal and extrasinusoidal constrictor response of hepatic microcirculation to endothelin-1 (ET-1) and endothelin-3 (ET-3) and the possible role of Ito cells vs. Kupffer cells or endothelial cells in mediating this response, using isolated rat livers under high-power intravital microscopy. Rats were pretreated by injection of 2.6 x 10(8) fluorescent latex beads (1 micron) intravenously to label Kupffer cells. Three hours later livers were isolated and perfused before and during the infusion of 1 nM ET-1 or ET-3 with or without the endothelin type A (ETA) receptor antagonist BQ-123 or ETB antagonist IRL-1038. Alternatively, the perfused livers were infused with the ETB agonist sarafotoxin 6c (S6c, 1 or 5 nM). Sinusoid diameters were quantitated at the sites of Ito cells (identified by vitamin A fluorescence) or Kupffer cells (phagocytosed fluorescent latex beads) or where neither cell type was found (endothelial cells). ET-1 was found to induce significant sinusoid constriction at the sites of Ito cells (13.21 +/- 0.58 microns control vs. 10.47 +/- 0.48 microns during ET-1 infusion) but not at the sites of Kupffer cells or endothelial cells (13.26 +/- 0.79 vs. 12.92 +/- 0.61 microns and 12.20 +/- 0.71 vs. 11.98 +/- 0.40 microns, respectively), whereas neither ET-3 nor S6c had any effect on sinusoid narrowing, despite a 1.8-fold (ET-3) or 5.6-fold (5 nM S6c) greater increase in total portal resistance compared with ET-1. (ABSTRACT TRUNCATED AT 250 WORDS)
Anterior/Posterior Spinal Instrumentation Versus Posterior Instrumentation Alone for the Treatment of Adolescent Idiopathic Scoliotic Curves More Than 90°

Study Design. A retrospective review of patients with adolescent idiopathic scoliosis (AIS), with curves more than 90° treated with either a combined anterior/posterior spinal fusion or a posterior spinal fusion alone. Objectives. To assess the results of spinal fusion for AIS curves >90° and determine whether the use of a posterior-only approach with an all-pedicle screw construct can decrease the need for anterior release surgery. Summary of Background Data. Treatment of AIS curves >90° often consists of anterior release and posterior fusion to improve coronal correction and fusion rate. However, the use of pedicle screws has allowed improved coronal curve correction rates even in large curves, which may decrease the need for anterior release surgery. Methods. A total of 54 consecutive patients with AIS with curves >90° who underwent a spinal fusion procedure at 1 institution between 1987 and 2001, with either a combined anterior/posterior spinal fusion (hooks and screws) or a posterior spinal fusion alone with an all-pedicle screw construct, were included for analysis. All patients had a minimum 2-year follow-up (mean 6.0; range 2.0–14.5), and were analyzed radiographically as well as with pulmonary function tests. Statistical analyses were performed between groups using the Wilcoxon-Mann-Whitney tests. Results. There were 20 patients treated with an anterior/posterior spinal fusion and 34 with a posterior spinal fusion alone. There were no statistically significant differences between the groups for gender, age, number of levels fused, preoperative coronal/sagittal Cobb measurements, coronal curve flexibility, or amount of postoperative coronal Cobb correction. There was less of a negative effect on pulmonary function in the group treated with posterior spinal fusion versus the group treated with a combined anterior/posterior spinal fusion (P < 0.005). There were no complications/reoperations in either group. Conclusion. In this patient population with often restrictive preoperative pulmonary function, a posterior-only approach with the use of an all-pedicle screw construct has the advantage of providing the same correction as an anterior/posterior spinal fusion, without the need for entering the thorax and more negatively impacting pulmonary function.
Prevalence of Methicillin-Resistant Staphylococcus aureus Colonization and Infection in Hospitalized Palliative Care Patients With Cancer

Little is known about the pattern of methicillin-resistant Staphylococcus aureus (MRSA) colonization and infection in hospitalized palliative care (PC) patients. We reviewed 854 admissions for 289 patients with advanced cancer managed by a PC service in a tertiary care hospital. The MRSA screening was performed at least once in 228 (79%) patients, and 21 (9%) of them were MRSA positive. Other cultures were done in 251 (86.8%) patients, and 8 (3%) patients were MRSA positive. The total number of MRSA-positive admissions was 28 (3%), with a median admission duration of 8 days. A substantial proportion of hospitalized PC patients with cancer are MRSA positive. Research is required to study the impact of infection control measures on the quality of PC delivered to MRSA-positive terminally ill patients in hospitals.