Miracle-dz/UniFAR-FTUs huggingface dataset:
Propofol and Dexmedetomidine for Monitored Anesthesia Care During Laser In Situ Keratomileusis

Background: The safety and efficacy of dexmedetomidine during laser in situ keratomileusis (LASIK) in stressed patients under topical anesthesia has not been fully investigated. Methods: We randomized 30 adult patients with ametropia, with a Self-Rating Anxiety Scale score greater than 50, who were undergoing LASIK to receive dexmedetomidine (DEX group), propofol (P group), or placebo. No differences in baseline characteristics were observed between the groups. In the DEX group, dexmedetomidine was loaded (0.3 &mgr;g/kg) before superficial anesthesia for eye surgery. Mean arterial pressure, heart rate (HR), oxygen saturation (SpO2), bispectral index (BIS) monitoring, and end-tidal CO2 were recorded before infusion (baseline) and every 5 minutes after loading of the study drugs. The degree of sedation was evaluated using the Ramsay Sedation Scale by an anesthesiologist who was unaware of the type of sedative used. The satisfaction of the patients and surgeon was assessed immediately after surgery using a 7-point Likert-like verbal rating scale. Results: Significantly lower HR was observed over time in the DEX group compared with the P group. No significant differences in SpO2 were observed between the groups. Bispectral index scores decreased at 10, 15, and 20 minutes after infusion. The surgeon's satisfaction scores were higher in the DEX group than in the P group. Recovery times were longer in DEX group than in the P group. Conclusions: Dexmedetomidine in conjunction with topical anesthesia offered better conscious sedation for nervous patients during LASIK, but these patients required a longer recovery period.
Cellphone evolution - applying evolution theory to an info-communication system

In this paper we propose the application of evolutionary principles in studying technological devices and processes. First we introduce the concepts of Darwinian evolution, which we use to draw an analogy with technology and show how human-made devices can be treated as evolving artificial agents. After explaining the methodology of reconstructing evolutionary history, we apply successfully the delineated principles and methodology to an info-communication system: cellphones. By modeling a possible phylogenetic tree of mobile phones and exploring technological changes through time, we are able to show evolutionary trends and parallels with biological processes.
COL1A1 mutation analysis in Lithuanian patients with osteogenesis imperfecta.

Osteogenesis imperfecta (OI) is a generalised disorder of connective tissue characterised by an increased fragility of bones and also manifested in other tissues containing collagen type I, by blue sclera, hearing loss, dentinogenesis imperfecta, hyperextensible joints, hernias and easy bruising. OI is dominantly inherited and results in >90% OI cases, caused by mutations in one of the two genes COL1A1 or COL1A2 coding for type I procollagen. The Lithuanian OI database comprises 147 case records covering the period of 1980 - 2001. Clinical and genealogical analysis of OI cases/families from Lithuania available for examination revealed 18 familial cases of OI type I and 22 sporadic cases: OI type II (3 cases), OI type III (11 cases) and OI type I (8 cases). As a result of their molecular genetic investigation, 11 mutations were identified in the COL1A1 gene in 13 unrelated patients. Of them, nine mutations (E500X, G481A, c.2046insCTCTCTAG, c.1668delT, c.1667insC, c.4337insC, IVS19+1G > A, IVS20-2A > G, IVS22-1G > T) appeared to be novel, i.e. not yet registered in the Human Type I and Type III Collagen Mutations Database (http://www.le.ac.uk/genetics/collagen).
A method comparison in monitoring disease progression of G93A mouse model of ALS

Our aim was to find an optimal method for monitoring disease progression and assessing the effectiveness of new agents in SOD1 mice. We compared six testing methods including clinical grading, weighing, hanging wire test, rotarod test, motor neuron counting and motor unit number estimation (MUNE) in SOD1 mice and control animals. The six methods were all able to differentiate between control animals and SOD1 transgenic mice at some time points; of them motor neuron counting, weighing and MUNE could detect abnormalities in presymptomatic stage in SOD1 mice; The number of functional motor units precisely correlated with motor neuron counts (r = 0.894, p<0.01). We concluded that MUNE is the better testing measure for monitoring disease and evaluating pharmacological therapies in SOD1 mice, which not only can reflect a meaningful biological effect of the agents used, but also is a sensitive, accurate and practical method.
Protective effect of resveratrol against neuronal damage through oxidative stress in cerebral hemisphere of aluminum and fluoride treated rats

Aluminum has no defined biological function and it is potentially involved in the pathogenesis of neurodegenerative disorders. Furthermore, the presence of fluoride causes more aluminum to accumulate in the brain, resulting in increased neuronal damage. In recent years, resveratrol through its ameliorative effects was found to be a neuroprotectant. This study reports the protective effects of resveratrol on combined aluminum and fluoride induced neuronal damage through oxidative stress in rats. Protective effects of resveratrol (30 mg/kg b.w) on markers of oxidative stress were determined in rats exposed to aluminum chloride (100 mg/kg b.w) along with sodium fluoride (10 mg/kg b.w) for 8 weeks. The results showed a statistically significant (p<0.05) increase in lipid peroxidation (LPx) as well as a significant (p<0.05) decrease in superoxide dismutase and catalase activity. Enlarged cells, neurofibrillary tangles, and vacuolar spaces showing oxidative stress in the cerebral cortex were also observed in hematoxylin and eosin stained sections in aluminum and fluoride treated rats. Administration of resveratrol along with aluminum + fluoride showed significant reversal of oxidative stress and neuronal damage in rats. Thus resveratrol potentially acts as a neuroprotectant against aluminum chloride + sodium fluoride induced neuronal damage through its anti-oxidant efficacy.
Immunohistochemical Analysis of Biliary Tract Lesions

The distinction among inflammatory, benign, and malignant lesions of the biliary tract can at times be difficult. Several methods have been used, including immunohistochemistry (IHC), with variable success. We evaluated a panel of IHC stains to determine their utility in discriminating between bile duct lesions. Formalin-fixed, paraffinembedded 4-μm sections from 12 inflammatory lesions, 10 bile duct adenomas, and 13 bile duct carcinomas were immunostained using a modified avidin-biotin-complex technique after epitope enhancement using antibodies for p53, Ki-67, and bcl-2. For p53 and bcl-2, greater than 1% of cells staining positive was interpreted as positive. The proliferation index was calculated by determining the number of Ki-67–positive cells in a 1000 cell count. In the inflammatory group, 0 of 12 reacted with anti-p53, 2 of 12 were positive with anti–bcl-2, and the proliferation index with was 22.9% ± 3.9%. Two of 10 bile duct adenomas showed reactivity with anti–bcl-2, and none were decorated with anti-p53 or Ki-67. In the carcinoma group, 6 of 13 were positive with anti-p53, 9 of 12 were positive with anti-bcl-2, and the proliferation index was 35.3% ± 5.5%. The proliferation rates differed significantly between groups (P < 0.05). The presence of bcl-2 and p53 immunoreactivity coupled with a high proliferative rate in a biliary tract lesion suggests a malignant process. A panel using these antibodies may be useful in difficult cases.
Subcellular Origin of Sphingosine 1-Phosphate Is Essential for Its Toxic Effect in Lyase-deficient Neurons*

Cerebellar granule cells from sphingosine 1-phosphate (S1P) lyase-deficient mice were used to study the toxicity of this potent sphingolipid metabolite in terminally differentiated postmitotic neurons. Based on earlier findings with the lyase-stable, semi-synthetic, cis-4-methylsphingosine phosphate, we hypothesized that accumulation of S1P above a certain threshold induces neuronal apoptosis. The present studies confirmed this conclusion and further revealed that for S1P to induce apoptosis in lyase-deficient neurons it must also be produced by sphingosine-kinase2 (SK2). These conclusions are based on the finding that incubation of lyase-deficient neurons with either sphingosine or S1P results in a similar elevation in cellular S1P; however, only S1P addition to the culture medium induces apoptosis. This was not due to S1P acting on the S1P receptor but to hydrolysis of S1P to sphingosine that was phosphorylated by the cells, as described before for cis-4-methylsphingosine. Although the cells produced S1P from both exogenously added sphingosine as well as sphingosine derived from exogenous S1P, the S1P from these two sources were not equivalent, because the former was primarily produced by SK1, whereas the latter was mainly formed by SK2 (as also was cis-4-methylsphingosine phosphate), based on studies in neurons lacking SK1 or SK2 activity. Thus, these investigations show that, due to the existence of at least two functionally distinct intracellular origins for S1P, exogenous S1P can be neurotoxic. In this model, S1P accumulated due to a defective lyase, however, this cause of toxicity might also be important in other cases, as illustrated by the neurotoxicity of cis-4-methylsphingosine phosphate.
Evaluating Diabetes Educational Interventions With a Skill Development Component in Adolescents With Type 1 Diabetes

Purpose Adolescents with type 1 diabetes mellitus (T1DM) may have reduced quality of life (QOL) when compared with their peers. This systematic review evaluated the effectiveness of diabetes self-management education (DSME) interventions with a skills development component on the QOL of adolescents with T1DM. Methods Six databases were systematically searched for studies on the QOL outcomes of DSME interventions for adolescents with T1DM. Fourteen studies published between 1994 and 2014 met the inclusion criteria. Results Of the 14 studies, only 4 had significant QOL outcomes for the intervention participants. Successful DSME interventions had indirect behavioral skills foci or a combination of indirect and direct behavioral skills foci and a duration ≥2 months. Conclusion This review provides evidence that educational interventions with an indirect behavioral skills development that facilitates diabetes management may improve QOL among adolescents with T1DM. Structured interventions targeting adolescents’ QOL are needed to determine which are most effective in improving QOL. Adolescents with T1DM should be routinely screened and monitored for low QOL and referred to QOL-improving interventions.
Evaluation of the Role of ALDH1 as Cancer Stem Cell Marker in Colorectal Carcinoma: An Immunohistochemical Study.

INTRODUCTION Colorectal Carcinoma (CRC) is the third most commonly diagnosed cancer in males. Stem Cells (SC) may be involved in tumour growth, including colon cancer. Aldehyde Dehydrogenase 1 (ALDH1) is a detoxifying enzyme that might modulate SC proliferation. AIMS To evaluate the immunohistochemical expression of ALDH1 as stem cell marker in the pathogenesis of colorectal carcinoma. MATERIALS AND METHODS This retrospective study included 71 colorectal specimens (49 colorectal carcinoma, 13 adenoma and 9 normal cases) that were collected from Pathology Department, Faculty of Medicine, Menoufia University during the period from 2011 to 2015. All cases were stained by ALDH 1 antibody. Survival data were available for 31cases. RESULTS There was a statistical significant association between epithelial positivity of ALDH1 and younger age (p=0.003), right sided tumour (p=0.038), presence of lymph node invasion (p= 0.04), ulcerating gross picture (p=0.01) and presence of vascular invasion (p=0.05). Moreover, there was statistical significant association between stromal positivity of ALDH1 and smaller tumour size (p=0.03) and inverse association between stromal expression of ALDH1 and grade of tumour (p=0.000) and perineural invasion (p= 0.05). Furthermore, there was an inverse significant relation between CD44 and ALDH1 expression (p=0.001). Univariate recurrence free survival analysis revealed the bad prognostic impact of high grade (p=0.03) and female sex (p=0.02) on patient outcome. CONCLUSION Epithelial expression of ALDH1 might be associated with poor prognosis while its stromal expression might be associated with good prognosis.
Moral Management in Competitive Markets

The intrinsic motivation of a firm's management for engaging in pro-social behavior is an important determinant of a firm's social conduct. I provide the first model in which firms run by morally-motivated managers engage in corporate social responsibility (CSR) in a competitive setting. CSR induced by moral management crowds out a competitor's strategic CSR, increasing profitability and leading shareholders to strategically delegate moral managers. Firms run by moral managers can engage in a socially-excessive amount of CSR, and shareholders appoint such managers if and only if moral management is sufficiently effective at crowding out a competitor's strategic CSR.
Investigating the preferences of older people for telehealth as a new model of health care service delivery: A discrete choice experiment

Introduction Telehealth approaches to health care delivery can potentially improve quality of care and clinical outcomes, reduce mortality and hospital utilisation, and complement conventional treatments. However, substantial research into the potential for integrating telehealth within health care in Australia, particularly in the provision of services relevant to older people, including palliative care, aged care and rehabilitation, is lacking. Furthermore, to date, no discrete choice experiment (DCE) studies internationally have sought the views and preferences of older people about the basic features that should make up a telehealth approach to these services. Methods Using a DCE, we investigated the relative importance of six salient features of telehealth (what aspects of care are to be pursued during telehealth sessions, distance to the nearest hospital or clinic, clinicians’ attitude to telehealth, patients’ experience of using technology, what types of assessments should be conducted face-to-face versus via telehealth sessions and the costs associated with receiving telehealth). Data were obtained from an online panel of older people aged 65 years and above, drawn from the Australian general population. Results The mean age for 330 study participants was 69 years. In general, individuals expressed strong preferences for telehealth services that offered all aspects of care, were relatively inexpensive and targeted specifically at individuals living in remote regions without easy access to a hospital or clinic. Participants also preferred telehealth services to be offered to individuals with some prior experience of using technology, provided by clinicians who were positive about telehealth but wanted all or some pre-telehealth health assessments to take place in a hospital or clinic. Preferences only differed by gender. Additionally, respondents did not feel that telehealth led to loss of privacy and confidentiality. Discussion Our findings indicate a preference amongst respondents for face-to-face pre-telehealth health assessments and, thereafter, a comprehensive telehealth model (in terms of services offered) targeted at those with some technological know-how as a substitute for attendance at hospitals and clinics, especially where these health facilities were far away from older people’s homes. The findings may be usefully incorporated into the design of future telehealth models of service delivery for older people.
Inhibition in kainate-lesioned hyperexcitable hippocampi: physiologic, autoradiographic, and immunocytochemical observations

Following kainate lesions of hippocampal subfield CA3, the remaining CA 1 pyramidal cells become hyperexcitable. This lesion is of interest because, morphologically, it resembles the damage often seen in cases of temporal lobe epilepsy; it may provide insight into the consequences of such cell loss in humans. The hyperexcitability in CA 1 is associated with a loss of both early and late IPSPs. At long postlesion latencies (2–4 months) inhibition shows partial recovery and the hyperexcitability subsides. The intent of the present work was to determine if alterations in CA 1 excitability and functional inhibition postlesion are correlated with changes in morphologic and physiologic indicators of inhibitory interneuron function or with alterations in binding sites for inhibitory transmitters. Using GAD immunocytochemistry, we found no acute or chronic lesion-induced decrease in numbers of CA 1 interneurons or in qualitative characteristics of the pericellular distribution of their terminals in CA 1 stratum pyramidale. Intracellular recordings from identified cells in CA 1 indicated that putative interneurons were viable in hyperexcitable tissue. It was further observed that “recovery” in tissue studied 2–4 months postlesion primarily involved the early IPSP; the late IPSP failed to reappear. Quantitative in vitro autoradiographic analysis of 3H-flunitrazepam--a marker for the early IPSP associated GABAA receptor complex--indicated that hyperexcitability was associated with an increase in GABAA receptor number in CA 1; receptor binding returned to normal at long postlesion latencies as the early IPSP returned and hyperexcitability subsided. Finally, hyperexcitable pyramidal cells were found to retain their responsivity to exogenously applied GABA. These data indicate that much of the cellular machinery necessary for inhibition is retained in CA 1, despite lesion-induced hyperexcitability. We suggest that the acute loss of the IPSP after kainate lesion is due to a transient disconnection between inhibitory and excitatory elements in CA 1 and/or to a loss of normal afferent drive from CA3 onto some CA 1 interneurons. We further suggest that incomplete recovery can be explained by abnormalities that occur as neuroplastic rearrangements in response to deafferentation of CA 1. The relevance of these studies to human hippocampal necrosis and to other models of focal epilepsy is discussed.
Incorporating action research into in-service teacher education (INSET) programs

Conducting action research (AR) is considered important for teachers’ professional development, their actual teaching practice and their theoretical knowledge . However, for many teachers, research is a new challenge and action research an unfamiliar form of classroom research. AR is a process through which practitioners address issues of importance in their practice. The interface between AR and teacher cognition can advance our understanding of teaching practice in general. Very few, if any, however, has investigated teachers’ deliberate decision-making processes after action research processes. In an attempt to address this gap in literature, this exploratory research study investigates the influence of AR on teacher cognition.
Design and Demonstration of a Microbiaxial Optomechanical Device for Multiscale Characterization of Soft Biological Tissues with Two-Photon Microscopy

Abstract The biomechanical response of tissues serves as a valuable marker in the prediction of disease and in understanding the related behavior of the body under various disease and age states. Alterations in the macroscopic biomechanical response of diseased tissues are well documented; however, a thorough understanding of the microstructural events that lead to these changes is poorly understood. In this article we introduce a novel microbiaxial optomechanical device that allows two-photon imaging techniques to be coupled with macromechanical stimulation in hydrated planar tissue specimens. This allows that the mechanical response of the microstructure can be quantified and related to the macroscopic response of the same tissue sample. This occurs without the need to fix tissue in strain states that could introduce a change in the microstructural configuration. We demonstrate the passive realignment of fibrous proteins under various types of loading, which demonstrates the ability of tissue microstructure to reinforce itself in periods of high stress. In addition, the collagen and elastin response of tissue during viscoelastic behavior is reported showing interstitial fluid movement and fiber realignment potentially responsible for the temporal behavior. We also demonstrate that nonhomogeneities in fiber strain exist over biaxial regions of assumed homogeneity.
The Changing Politics of American Federalism

This article synthesizes and interprets contemporary research on the changing relationships between American federalism and the U.S. political system. It does so by examining how three important political trends—nationalization, polarization, and delegitimation—have affected intergovernmental relations and intergovernmental policy across a range of venues: electoral politics, policymaking, and public administration. In each of these areas, traditional scholarly perspectives on the relationship between federalism and the political system are contrasted with the findings of contemporary research and analysis.
Therapeutic effects of Citrullus colocynthis fruit in patients with type II diabetes: A clinical trial study

Aim: Citrullus colocynthis plant was traditionally used for the treatment of diabetes in Sabzevar city, Iran. This study aimed to investigate the effects of C. colocythis on fasting blood sugar (FBS) and HbA1c in patients with type II diabetes. Materials and Methods: Totally 70 patients with type II diabetes attending the diabetes clinic in Sabzevar city were investigated. Patients were divided into two groups of intervention and placebo randomly and were studied for 2 months. Mean serum level of FBS and HbA1c was compared within and between groups at the end of the study. Data were analyzed using student and paired t-tests via SPSS software version 16. Results: A significant difference was revealed between before and after intervention for HbA1c and FBS levels in the intervention group (P = 0.01 and P = 0.04, respectively). The difference between before and after intervention for FBS and HbA1c levels in the placebo group were not significant (P = 0.8, P = 0.68 respectively). This study showed a negative relationship between either the mean ± standard deviation serum level of FBS or HbA1c and body mass index in the treatment group significantly (P = 0.03, 0.008, respectively). The present study did not identify any side effects during the study period among the treatment group. Conclusion: According to the findings of this study, application of 125 mg C. colocynthis once per day for 2 months can lead to considerable decrease in the mean levels of HbA1c and FBS among the patients with type II diabetes without any side effects.