Miracle-dz/UniFAR-FTUs huggingface dataset:
Parathyroid hormone gene rs6256 and calcium sensing receptor gene rs1801725 variants are not associated with susceptibility to colorectal cancer in Iran.

BACKGROUND Substantial evidence from epidemiological studies has suggested that increased levels of calcium may play a protective role against colorectal cancer (CRC). Given the vital role of calcium sensing receptor (CaSR) and parathyroid hormone (PTH) in the maintenance of calcium homeostasis, we explored whether the rs1801725 (A986S) variant located in exon 7 of the CaSR gene and the rs6256 variant located in exon 3 of PTH gene might be associated with CRC risk. MATERIALS AND METHODS In this study 860 subjects including 350 cases with CRC and 510 controls were enrolled and genotyped using PCR-RFLP methods. RESULTS We observed no significant difference in genotype or allele frequencies between the cases with CRC and controls for both CaSR and PTH genes either before or after adjustment for confounding factors including age, BMI, sex, smoking status, and family history of CRC. Furthermore, no evidence for effect modification of any association of rs1801725 and rs6256 variants and CRC by BMI, sex, or tumor site was observed. In addition, there was no significant difference in genotype and allele frequencies between the normal weight (BMI<25 kg/m2) cases and overweight/ obese (BMI≥25 kg/m2) cases for the two SNPs. CONCLUSIONS These data indicated that the CaSR gene A986S variant is not a genetic contributor to CRC risk in the Iranian population. Furthermore, our results suggest for the first time that PTH gene variant does not affect CRC risk. Nonetheless, further studies with larger sample size are needed to validate these findings.
Using threshold values for cost per quality-adjusted life-year gained in healthcare decisions

Background: In many countries, the incremental cost-effectiveness ratio (ICER) is used to assess whether an intervention is worth its costs. At the same time, policy makers often feel uncomfortable with refusing reimbursement of any intervention purely on the basis of the fact that the ICER exceeds a specific threshold value. Reluctance to define a single threshold value for the ICER seems to have been stronger in social security systems than in national healthcare services systems. This study explores how basic differences between healthcare systems impact upon the potential usefulness of an ICER threshold value. Methods: This study is a narrative review of literature about the theoretical foundations of the ICER threshold value approach and its practical relevance in different types of healthcare systems. Results: A single ICER threshold value cannot be maintained, defined, or measured and should not be used as a policy-making tool. None of the solutions presented up until now to make the ICER threshold approach a valuable policy-making tool overcome the important weaknesses of the approach. Conclusions: ICERs and ICER threshold values are insufficient for assessing interventions' value for money. Rather, they should be considered as one element in the decision-making process. Complete rationalization of the decision-making process by means of quantitative decision criteria is undesirable and not feasible. Increasing transparency in the criteria used for a decision and explicitness about the relative importance of each criterion should, therefore, be the major goal.
Relationship Between External and Internal Loads of Professional Soccer Players During Full Matches in Official Games Using Global Positioning Systems and Heart-Rate Technology.

PURPOSE To analyze the match running profile, distance traveled over successive 15 min of match play, heart rates (HRs), and index of performance efficiency (effindex) of professional soccer players with a global positioning system (GPS) and HR in official competition. METHODS Twenty-six professional players were investigated during full matches in competitive club-level matches (N = 223). Time-motion data and HR were collected using GPS and HR technology. RESULTS The relative total distance was 113 ± 11 m/min, with substantial differences between halves. For all playing positions, a substantial decrease in total distance and distance covered at >13.0 km/h was observed in the second half in comparison with the first. The decrease during the second half in distance covered at >13.0 km/h was substantially higher than in total distance. The average HR recorded was 86.0% maximal HR, and the relationship between external and internal load (effindex) was 1.3, with substantial differences between halves in all playing positions, except strikers for effindex. Wide midfielders reflected substantially the lowest mean HR and highest effindex, whereas center backs showed substantially the lowest effindex of all playing positions. CONCLUSIONS The current study confirmed the decrement in a player's performance toward the end of a match in all playing positions. Wide midfielders displayed the highest and fittest levels of physical and physiological demands, respectively, whereas center backs had the lowest and least-fit levels of physical and physiological demands, respectively. The position-specific relationship between external and internal load confirms that players with more overall running performance during the full match were the best in effindex.
Effects of Neonatal Handling on Central Noradrenergic and Nitric Oxidergic Systems and Reproductive Parameters in Female Rats

Early-life environmental events that disrupt the mother-pup relationship may induce profound long-lasting changes on several behavioral and neuroendocrine systems. The neonatal handling procedure, which involves repeated brief maternal separations followed by experimental manipulations, reduces sexual behavior and induces anovulatory estrous cycles in female rats. On the afternoon of proestrus, neonatally handled females show a reduced surge of luteinizing hormone (LH) and an increased content of gonadotropin-releasing hormone in the medial preoptic area (MPOA). In order to detect the possible causes for the reduced ovulation and sexual behavior, the present study aimed to analyze the effects of neonatal handling on noradrenaline (NA) and nitric oxide (NO) levels in the MPOA on the afternoon of proestrus. Neonatal handling reduced MHPG (NA metabolite) levels and MHPG/NA ratio in the MPOA, indicating decreased NAergic activity. Additionally, neonatal handling decreased NO levels, as measured by the metabolites (NOx), nitrite and nitrate in the same period. We may conclude that the neonatal handling procedure decreased activity of the NAergic and NOergic systems in the MPOA during proestrus, which is involved in the control of LH and FSH secretion, and this may possibly explain the anovulatory estrous cycles and reduced sexual behavior of the neonatally handled female rats.
A Cross-reactivity Study of Whole Genomic DNA Probes for Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis

In this study, we evaluated the sensitivity and specificity of whole genomic DNA probes for the periodontal pathogens Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis. By means of these probes, DNA hybridizations were performed against other organisms found in the oral cavity and organisms previously determined to be genetically similar. All three probes were sensitive to 103 cells for their respective organism. The H. actinomycetemcomitans probe cross-reacted with several haemophilus strains, Wolinella, and Campylobacter, indicating that H. actinomycetemcomitans-specific sequences would have to be identified and cloned for accurate detection of this organism in heterogeneous patient samples. Only very low levels of cross-reactivity were observed between the B. intermedius probe and representative black-pigmented Bacteroides. This low level of cross-reactivity did not interfere with the accurate identification of B. intermedius in sample evaluations. The B. gingivalis probe showed no cross-reactivity. Whole genomic probes will be used for the detection of B. intermedius and B. gingivalis in patient samples.
Assessment of Postoperative Pain in Cats After Ovariectomy by Laparoscopy, Median Celiotomy, or Flank Laparotomy.

OBJECTIVE To compare postoperative pain, duration of surgery, and duration of anesthesia for 3 methods of ovariectomy in cats: (1) conventional ventral median open approach (Midline), (2) right flank approach (Flank), and (3) median 2-portal laparoscopic procedure (Lap). STUDY DESIGN Randomized, prospective clinical trial. ANIMALS Healthy, sexually intact female cats (n = 60). METHODS Cats were randomly assigned to 1 of 3 groups: Midline (n = 20), Flank (20), and Lap (20) were evaluated 1, 2, 4, 6, and 12 hours after endotracheal extubation. Postoperative pain was scored using the 4A-vet pain scale that combines a subjective numerical pain rating and objective scoring of physiologic and behavioral variables including the response to stimulation of the surgical site. Pain scores (PS) were compared between groups. RESULTS There was a significant difference in the PS between groups. PS for Midline and Flank were not significantly different but were both significantly higher compared with Lap. Depending on time, 5-20% of the cats had intense postoperative pain in both Midline and Flank groups. None of the Lap cats had intense postoperative pain. CONCLUSIONS Laparoscopic ovariectomy, although slower, appeared less painful compared with conventional ventral midline and flank ovariectomy. Postoperative pain did not differ significantly between midline and flank groups.
Rat sertoli and spermatogenic cells express a similar gene, and its product is antigenically related to an outer dense fiber‐associated protein

We have previously reported that a heterodimeric protein secreted by rat Sertoli cells is antigenically related to a protein associated with outer dense fibers of the sperm tail. Therefore, we have explored the possibility that Sertoli and spermatogenic cells express a similar gene encoding a homologous protein. A Sertoli cell heterodimeric protein cDNA probe recognizes specific mRNA in pachytene and round spermatids fractionated by centrifugal elutriation; however, this specific mRNA was less prominent than in cultured Sertoli cells. In agreement with these observations, in situ hybridization experiments show that Sertoli cells are predominantly engaged in active heterodimeric protein mRNA synthesis, while meiotic prophase spermatocytes and spermatids also show significant but less abundant specific mRNA. Immunoblotting experiments demonstrate that, while Sertoli cells synthesize a heterodimeric protein consisting of two disulfidelinked components with molecular masses of 45 and 35 kD, both primary spermatocytes and round spermatids synthesize single 30 kD monomers not associated by disulfide linkage but recognized by antisera to Sertoli cell heterodimeric protein Immunoblotting and immunogold electron microscopic studies show that antisera to Sertoli cell heterodimeric protein recognize a protein associated with outer dense fibers. This immunoreactivity was abolished by a 5‐min pronase treatment, without affecting the integrity of outer dense fibers. Results of this study and previous studies demonstrate that both Sertoli and spermatogenic cells express a similar gene and that an antigenically related product encoded by this gene becomes associated with outer dense fibers during their assembly at spermiogenesis. © 1992 Wiley‐Liss, Inc.
A Novel Combined Method of Osteosynthesis in Treatment of Tibial Fractures: A Comparative Study on Sheep with Application of Rod‐Through‐Plate Fixator and Bone Plating

The study compares the efficiency of a new bone fixator combining periostal and intramedullary osteosynthesis to bone plating in treatment of tibial fractures in sheep. Experimental osteotomies were performed in the middle third of the left tibia. Animals were divided into two groups: in one group (four animals) combined osteosynthesis (rod‐through‐plate fixator, RTP fixator) was applied, and in the other group (three animals) bone plating was used. The experiments lasted for 10 weeks during which fracture union was followed by radiography, and the healing process was studied by blood serum markers reflecting bone turnover and by histological and immunohistochemical investigations. In the RTP fixator group, animals started to load body weight on the operated limbs the next day after the surgery, while in the bone plating group, this happened only on the seventh day. In the RTP fixator group, consolidation of fractures was also faster, as demonstrated by radiographical, histological, and immunohistochemical investigations and in part by blood serum markers for bone formation. It can be concluded that application of RTP fixation is more efficient than plate fixation in the treatment of experimental osteotomies of long bones in sheep.
Do Lower Top Marginal Tax Rates Slow the Income Growth of Workers?

Top marginal tax rates are positively correlated with the pretax income growth of the bottom 90 per cent — those who are not subject to the top rates. To explain this correlation, this paper presents and tests a model in which executives can increase firm profitability by (i) increasing the firm's level of technology and (ii) decreasing labor costs. In the model, higher marginal tax rates may reduce pretax inequality by increasing the average income growth of workers. This hypothesis is tested by examining the effect of top marginal tax rates on (unobserved) relative bargaining power between labor and firms and, therefore, on the income growth of workers in the USA. Bargaining power, in both the theoretical and the empirical models, is proxied by private‐sector unionization and use of offshore labor resulting in higher imports.
Transcriptional immune response in mesenteric lymph nodes in pigs with different levels of resistance to Ascaris suum

A single nucleotide polymorphism on chromosome 4 (SNP TXNIP) has been reported to be associated with roundworm (Ascaris suum) burden in pigs. The objective of the present study was to analyse the immune response to A. suum mounted by pigs with genotype AA (n = 24) and AB (n = 23) at the TXNIP locus. The pigs were repeatedly infected with A. suum from eight weeks of age until necropsy eight weeks later. An uninfected control group (AA; n = 5 and AB; n = 5) was also included. At post mortem, we collected mesenteric lymph nodes and measured the expression of 28 selected immune-related genes. Recordings of worm burdens confirmed our previous results that pigs of the AA genotype were more resistant to infection than AB pigs. We estimated the genotype difference in relative expression levels in infected and uninfected animals. No significant change in expression levels between the two genotypes due to infection was observed for any of the genes, although IL-13 approached significance (P = 0.08; Punadjusted = 0.003). Furthermore, statistical analysis testing for the effect of infection separately in each genotype showed significant up-regulation of IL-13 (P<0.05) and CCL17 (P<0.05) following A. suum infection in the ‘resistant’ AA genotype and not in the ‘susceptible’ AB genotype. Pigs of genotype AB had higher expression of the high-affinity IgG receptor (FCGR1A) than AA pigs in both infected and non-infected animals (P = 1.85*10−11).
Development of a Sensory Neuronal Cell Model for the Estimation of Mild Eye Irritation

In an attempt to improve the in vitro test strategy for the estimation of eye irritation, a neuronal cell model has been developed, with cells expressing vanilloid receptor type 1 (VR1) nociceptors. The currently accepted method for measuring eye irritancy is the ethically and scientifically criticised Draize rabbit eye test, despite the fact that alternative in vitro methods are available which have proved to be reliable and reproducible for predicting severe ocular toxicity. However, no alternative tests for measuring neuronal stimulation have yet been developed, and the prediction of eye irritation in the mild range is therefore insufficient. VR1 is a nociceptor localised in C-fibre neurons innervating the cornea and the surrounding tissue, and it responds to potentially damaging stimuli by releasing Ca2+ into the cytoplasm. As a sensory endpoint, [Ca2+]i was measured in VR1 transfected cells, as well as in control cells. Short-term cell cytotoxicity studies (cell membrane rupture and morphological divergence) were used to determine the non-corrosive concentrations of the test chemicals. Preliminary results indicated that hygiene products used daily may induce eye irritation via VR1 nociceptors. The lowest toxic concentration (0.025%) of liquid hand soap, as determined by morphologic divergences of cells, generated an 80% increase in [Ca2+]i over the basal [Ca2+]i in VR1 transfected cells, whereas the non-specific [Ca2+]i increased by 33%. Furthermore, all the endpoints studied indicated that shampoo for children was less active than shampoo for adults. If this method is successfully validated with standardised reference chemicals, the model could complete the test battery of in vitro alternatives, resulting in the saving of thousands of laboratory animals.
Effect of dual‐specificity protein phosphatase 5 on pluripotency maintenance and differentiation of mouse embryonic stem cells

The MAPK/Erk signaling pathway is considered as a key regulator of the pluripotency and differentiation of embryonic stem (ES) cells, while dual‐specificity protein phosphatases (DUSPs) are negative regulators of MAPK. Although DUSPs are potential embryogenesis regulators, their functions in the regulation of ES cell differentiation have not been demonstrated. The present study revealed that Dusp5 was expressed in mouse ES (mES) cells and that its expression was correlated with the undifferentiated state of these cells. Exogenous Dusp5 expression enhanced mES cell clonogenicity and suppressed mES cell differentiation by maintaining Nanog expression via the inhibition of the Erk pathway. Following Dusp5 knockdown, Nanog and Oct4 expression was significantly attenuated and the Erk signaling pathway was activated. Additionally, EBs derived from Dusp5 knockdown mES cells (KDEBs) exhibited a weak adherence capability, very little outgrowth, and a reduction in the number of epithelial‐like cells. The expression of Gata6 (an endodermal marker) and Flk1 and Twist1 (mesodermal markers) was inhibited in KDEBs, which indicated that Dusp5 influenced the differentiation of these germ layers during EB development. Collectively, this study suggested that Dusp5 plays an important role in the maintenance of pluripotency in mES cells, and that Dusp5 may be required for EB development. J. Cell. Biochem. 112: 3185–3193, 2011. © 2011 Wiley Periodicals, Inc.
The Impact of Agricultural Extension Services in the Context of a Heavily Subsidized Input System: The Case of Malawi

This paper examines the interplay between Malawi’s input subsidy and access to extension services, and the impact of both on farm productivity and food security using Malawi’s Integrated Household Panel Survey. A correlated random effects (CRE) device is used, and consistency and robustness of results are checked using various other estimation models. The receipt of fertilizer and seed subsidies is shown to have an inconsistent impact on farm productivity and food security; at the same time, access to agricultural advice is consistently insignificant in explaining these. Further analysis, however, shows a statistically significant and strong association with farm productivity and food security when access to extension services is unpacked to include indicators of usefulness and farmers’ satisfaction. Households that reported receipt of “very useful” agricultural advice had greater productivity and greater food security compared to those that reported receipt of advice that they considered not useful and those that did not receive any advice at all. This result implies the need to ensure the provision of relevant and useful agricultural advice to increase the likelihood of achieving agricultural development outcomes.
CEO Compensation and Risk-Taking in Banking Industry

La crise financiere de 2008 a ete largement imputee a une prise de risque excessive par lesbanques US et du monde entier, qui ont ete mises en grande difficulte. Une des questionsprincipales que se posent les scientifiques et les regulateurs concerne le role joue par lesmodes de remuneration des dirigeants des banques dans l'incitation a la prise de risque. Le butde cette recherche est d'etudier si les modes de remuneration des dirigeants des banquesinduisent la prise de risque et contribuent a la crise financiere. Nous nous proposonsd'analyser separement les effets de chaque composante de la remuneration des PDG (lesalaire, la prime, les autres remunerations annuelles, le pourcentage du salaire, le pourcentagede la prime, le pourcentage des autres remunerations annuelles, et les remunerations qui sontfondees sur une participation au capital de la banque) sur la prise de risque dans le secteurbancaire. Nous tenterons aussi de deceler plus specifiquement une eventuelle responsabilitede ces modes de remuneration dans le declenchement de la crise financiere et lesmanifestations du risque bancaire dans les deux premieres annees de crise. Pour les risquesbancaires, nous utilisons de nombreuses mesures differentes : le risque total, le risquesystematique, le risque idiosyncratique, le ratio de la provision pour pertes sur prets enpourcentage des credits, le ratio des prets non performants en pourcentage des credits, lerisque de defaut mesure par la distance par rapport au defaut (Z-score), le changement de laCDS, le changement de la notation des banques et la chute de la valeur des actions. Enutilisant un echantillon de 63 grandes banques d'Europe, du Canada et des Etats-Uniscouvrant une periode de 5 ans de 2004 a 2008, nous trouvons que le salaire et la prime desPDG diminuent l‘essentiel des risques bancaires, alors que les autres remunerations annuellesdes PDG les augmentent. Ces modes de remuneration des PDG n‘ont pourtant aucun lien avecles changements du risque bancaire dans la crise. En ce qui concerne les remunerationsfondees sur une participation au capital de la banque, nous trouvons que l'utilisation desactions gratuites en remuneration pendant la periode pre-crise n'a pas d'effet sur leschangements anormaux du risque bancaire dans la periode de crise. Au contraire, l'utilisationdes options d'achat d'actions pendant la meme periode est une des raisons des manifestationsdu risque bancaire dans la recente crise financiere.