Miracle-dz/UniFAR-FTUs huggingface dataset:
Inositol Pyrophosphate Kinase Asp1 Modulates Chromosome Segregation Fidelity and Spindle Function in Schizosaccharomyces pombe

ABSTRACT Chromosome transmission fidelity during mitosis is of critical importance for the fitness of an organism, as mistakes will lead to aneuploidy, which has a causative role in numerous severe diseases. Proper segregation of chromosomes depends on interdependent processes at the microtubule-kinetochore interface and the spindle assembly checkpoint. Here we report the discovery of a new element essential for chromosome transmission fidelity that implicates inositol pyrophosphates (IPPs) as playing a key role in this process. The protein is Asp1, the Schizosaccharomyces pombe member of the highly conserved Vip1 family. Vip1 enzymes are bifunctional: they consist of an IPP-generating kinase domain and a pyrophosphatase domain that uses such IPPs as substrates. We show that Asp1 kinase function is required for bipolar spindle formation. The absence of Asp1-generated IPPs resulted in errors in sister chromatid biorientation, a prolonged checkpoint-controlled delay of anaphase onset, and chromosome missegregation. Remarkably, expression of Asp1 variants that generated higher-than-wild-type levels of IPPs led to a faster-than-wild-type entry into anaphase A without an increase in chromosome missegregation. In fact, the chromosome transmission fidelity of a nonessential chromosome was enhanced with increased cellular IPPs. Thus, we identified an element that optimized the wild-type chromosome transmission process.
Morphologic variations of small cell lung cancer: A histopathologic study of pretreatment and posttreatment specimens in 104 patients

A consecutive series of 104 autopsies on patients treated in protocol for small cell carcinoma of the lung (SCCL) was studied with respect to (1) metastatic pattern at autopsy in relation to pretreatment WHO 1981 classification, and (2) extent and significance of non‐SCCL tumor tissue at autopsy. The only significant difference in the metastatic pattern at autopsy between patients with pretreatment oat cell or intermediate subtype was metastases to the brain. Thus, the frequency of brain metastases was 17/35 (49%) in patients with oat cell type compared to 2/18 (11%) in patients with intermediate type (P < 0.05). At autopsy 13 of 98 patients (13%) had non‐SCCL tumor tissue in at least one site. These patients had a significantly shorter survival (P = 0.020) compared to patients with pure SCCL at autopsy. Furthermore, none of these 13 patients had obtained complete remission. Whether these morphologic variations had been present at the pretreatment stage of the disease or were due to the chemotherapeutic treatment could not be solved in the present study. However, the results might indicate that mixed SCCL/non‐SCCL histology is a negative prognostic factor in the treatment of SCCL. Prospective studies including more extensive pretreatment tissue sampling seem to be required.
Neisseria gonorrhoeae Filamentous Phage NgoΦ6 Is Capable of Infecting a Variety of Gram-Negative Bacteria

ABSTRACT We constructed a phagemid consisting of the whole genome of the Neisseria gonorrhoeae bacteriophage NgoΦ6 cloned into a pBluescript plasmid derivative lacking the f1 origin of replication (named pBS::Φ6). Escherichia coli cells harboring pBS::Φ6 were able to produce a biologically active phagemid, NgoΦ6fm, capable of infecting, integrating its DNA into the chromosome of, and producing progeny phagemids in, a variety of taxonomically distant Gram-negative bacteria, including E. coli, Haemophilus influenzae, Neisseria sicca, Pseudomonas sp. , and Paracoccus methylutens. A derivative of pBS::Φ6 lacking the phage orf7 gene, a positional homolog of filamentous phage proteins that mediate the interaction between the phage and the bacterial pilus, was capable of producing phagemid particles that were able to infect E. coli, Haemophilus influenzae, N. sicca, Pseudomonas sp. , and Paracoccus methylutens, indicating that NgoΦ6 infects cells of these species using a mechanism that does not involve the Orf7 gene product and that NgoΦ6 initiates infection through a novel process in these species. We further demonstrate that the establishment of the lysogenic state does not require an active phage integrase. Since phagemid particles were capable of infecting diverse hosts, this indicates that NgoΦ6 is the first broad-host-range filamentous bacteriophage described.
Problem Based Learning on Cloud Economics Analysis Using Open Source Simulation

The paper reports the applicablity of open source simulation software called GreenCloud to assist a novel Problem Based Learning in a laboratory environment. The actual case of Indonesia government plan to deploy cloud based data center infrastructure was picked up as the actual case. In such case, cloud economics analysis is required along with technical one. An open source software called GreenCloud is suitebale to perform the simulation and analysis of cloud computing from economics perspective. It was applied into three models of cloud architecture namely Two-Tier, Three-Tier and Three-Tier High Speed and then analyzed in terms of their energy consumptions based on three options of cloud economics scheme, namely i) non energy savings, ii) Dynamics Voltage and Frequency Scaling (DVFS) and iii) Dynamics Shutdown (DNS).
Clinical and structural results of open repair of an isolated one-tendon tear of the rotator cuff.

BACKGROUND The clinical outcomes of open rotator cuff repair are well established, but the structural results and their effect on clinical outcome are poorly known. We assessed the structural changes in the musculotendinous units after open rotator cuff repair and correlated these findings with the clinical outcome to establish a benchmark for future series. METHODS Thirty-two consecutive standardized open repairs of a single tendon tear of the rotator cuff were analyzed in twenty-one men and eleven women with an average age of 59.0 years. The supraspinatus tendon was involved in twenty-two patients and the subscapularis tendon, in ten. The clinical outcome, including the Constant score, was assessed prospectively for all patients at an average of thirty-eight months postoperatively. The structural outcome was assessed on standardized magnetic resonance imaging scans. RESULTS The mean overall subjective shoulder value was 82.8% of the value for a normal shoulder. On the average, the age and gender-adjusted Constant score increased from 63.9% preoperatively to 94.5% postoperatively (p < 0.0001); the score for pain, from 6.8 points to 13.2 points (p < 0.0001); and the score for activities of daily living, from 11.2 points to 17.9 points (p < 0.0001). The overall rerupture rate was 13% (four of the thirty-two shoulders). All reruptures were distinctly smaller than the original tear. Muscular atrophy or fatty infiltration did not significantly decrease after the tendon repair. In fact, fatty infiltration in the supraspinatus (p < 0.0053) and infraspinatus (p < 0.003) muscles increased significantly. CONCLUSIONS Direct open repair of a complete, isolated tear of one tendon of the rotator cuff resulted in significant subjective and objective improvement and very high patient satisfaction. Successful direct repair was not associated with a decrease in preoperative muscular atrophy and was associated with increased fatty infiltration of the muscle.
KPIs for facility's performance assessment, Part II: identification of variables and deriving expressions for core indicators

Purpose – The purpose of this paper is to identify key variables that affect the quantifiable key performance indicators (KPIs) and to derive equations to measure these indicators. Qualitative KPIs are also discussed in terms of the aspects that need to be covered while carrying out qualitative performance assessment. Design/methodology/approach – A combination of literature and an industry opinion-based qualitative approach is applied to develop equations to calculate the quantifiable KPIs. A facility asset management consulting firm is included in the process of deriving the equations. Key aspects of a facility's qualitative performance assessment are categorized and discussed by performing a literature review. Findings – Mathematical expressions for core performance indicators are presented and discussed along with key variables. In addition, the information needed to quantify these core indicators is also discussed. Research limitations/implications – This paper represents the second step towards esta.
THE TENSION BETWEEN PROFESSIONAL CONTROL AND OPEN PARTICIPATION

Amid growing difficulties for professionals generally, media workers in particular are negotiating the increasingly contested boundary space between producers and users in the digital environment. This article, based on a review of the academic literature, explores that larger tension transforming the creative industries by extrapolating from the case of journalism – namely, the ongoing tension between professional control and open participation in the news process. Firstly, the sociology of professions, with its emphasis on boundary maintenance, is used to examine journalism as boundary work, profession, and ideology – each contributing to the formation of journalism's professional logic of control over content. Secondly, by considering the affordances and cultures of digital technologies, the article articulates open participation and its ideology. Thirdly, and against this backdrop of ideological incompatibility, a review of empirical literature finds that journalists have struggled to reconcile this key tension, caught in the professional impulse toward one-way publishing control even as media become a multi-way network. Yet, emerging research also suggests the possibility of a hybrid logic of adaptability and openness – an ethic of participation – emerging to resolve this tension going forward. The article concludes by pointing to innovations in analytical frameworks and research methods that may shed new light on the producer–user tension in journalism.
Differences in fatigability between the sexes during a sustained submaximal contraction protocol in prepubertal children

Abstract The aim of this study was to determine whether there are differences in the fatigability of plantar flexor muscles during sustained submaximal contractions in prepubertal boys and girls. Fifteen boys (age 10.0 ± 1.0 years) and 15 girls (age 9.8 ± 0.9 years) participated in the study. The fatigue protocol consisted of a 10 min isometric plantar flexion at 20% of the maximal voluntary contraction. Immediately after this, five maximal isometric contractions were performed with a 3 min interval between contractions. During the experiment, electromyograms of the agonist muscles soleus and medial gastrocnemius and antagonist tibialis anterior were recorded. We observed no differences between the sexes (P < 0.05) in the decrease in torque or in the recovery rate after the fatigue protocol. Nor were there any differences between the sexes (P < 0.05) in agonist or antagonist muscle activation during the fatigue protocol and recovery period. The results indicate that there are no differences in fatigability between prepubertal boys and girls during submaximal sustained contractions, probably because the agonist and antagonist muscles were activated similarly in both sexes.
Irisin mRNA and circulating levels in relation to other myokines in healthy and morbidly obese humans.

OBJECTIVE Skeletal muscle is considered to be an endocrine organ that secretes a number of myokines including follistatin (FST), myostatin (MSTN), activin A, and the newly identified irisin. Irisin's biology and function exhibit similarities with the functions of the FST-MSTN-activin A axis. It remains unknown whether there is any interplay among these molecules. The aim of this study is to examine potential associations of irisin with the FST, MSTN, and activin A axis. METHODS Two observational studies were performed to evaluate the associations of irisin with the other three peptides. Study A included 150 healthy males aged 18.48±0.16 years with BMI 23.18±3.75 kg/m(2). Fasting serum samples were used to measure the levels of the molecules of interest. Study B included 14 morbidly obese individuals, candidates for bariatric surgery, aged 53.14±8.93 years with BMI 50.18±10.63 kg/m(2). Blood samples were obtained after an overnight fast. Eight out of the 14 participants consented to an optional thigh biopsy during their bariatric surgery. Using the above blood and tissue samples, we measured circulating levels and muscle mRNA of irisin, FST, MSTN, and activin A. RESULTS We report that FNDC5 mRNA in muscle is positively correlated with FST mRNA expression in morbidly obese subjects (ρ=0.93, P<0.001). We also found that circulating irisin is positively correlated with FST circulating levels among lean subjects (ρ=0.17, P=0.05) while this association was suggestive among the obese (ρ=0.56, P=0.07). CONCLUSION The newly identified myokine irisin may be positively associated with FST at both the mRNA and circulating protein level.