Miracle-dz/UniFAR-FTUs huggingface dataset:
Contribution of antibody in normal human serum to early deposition of C3 onto encapsulated and nonencapsulated Cryptococcus neoformans

Encapsulated and nonencapsulated cryptococci differ in their activation of the complement system. Incubation of nonencapsulated cryptococci in normal human serum (NHS) initiates both the classical and alternative pathways. This activation is characterized by an immediate, synchronous activation and binding of C3 to the yeast cells. Encapsulated cryptococci activate only the alternative pathway. This activation is characterized by a delayed (4 to 5 min), asynchronous activation and binding of C3. We examined the properties of antibodies in NHS that mediate immediate, synchronous binding of C3 to nonencapsulated cryptococci and zymosan. Adsorption of NHS with nonencapsulated cryptococci or zymosan produced a 4- to 6-min delay in the kinetics for activation and binding of C3 from the adsorbed serum to each respective yeast cell. This delay was similar to the delay observed when nonencapsulated cryptococci or zymosan was incubated in NHS in which the classical pathway was blocked by chelation of Ca2+. Proteins bound to serum-treated nonencapsulated cryptococci or zymosan were eluted and found to be predominantly immunoglobulin G (IgG), with lesser amounts of IgM. The eluted IgG could restore to adsorbed serum the rapid early kinetics for activation and binding of C3 characteristic of classical pathway initiation. Cross-adsorption showed that there was considerable cross-reactivity between the antibodies which restored rapid, early activation kinetics to NHS adsorbed with zymosan or nonencapsulated cryptococci. Encapsulated cryptococci were unable to adsorb the antibodies from NHS that mediated the rapid, early activation and binding of C3 to zymosan and nonencapsulated cryptococci. The latter results show that occlusion of antigenic sites at the cryptococcal cell wall is a newly recognized property that can be added to the repertoire of biological activities of the cryptococcal capsule.
Retrieval Compensated Group Structured Sparsity for Image Super-Resolution

Sparse representation-based image super-resolution is a well-studied topic; however, a general sparse framework that can utilize both internal and external dependencies remains unexplored. In this paper, we propose a group-structured sparse representation approach to make full use of both internal and external dependencies to facilitate image super-resolution. External compensated correlated information is introduced by a two-stage retrieval and refinement. First, in the global stage, the content-based features are exploited to select correlated external images. Then, in the local stage, the patch similarity, measured by the combination of content and high-frequency patch features, is utilized to refine the selected external data. To better learn priors from the compensated external data based on the distribution of the internal data and further complement their advantages, nonlocal redundancy is incorporated into the sparse representation model to form a group sparsity framework based on an adaptive structured dictionary. Our proposed adaptive structured dictionary consists of two parts: one trained on internal data and the other trained on compensated external data. Both are organized in a cluster-based form. To provide the desired over-completeness property, when sparsely coding a given LR patch, the proposed structured dictionary is generated dynamically by combining several of the nearest internal and external orthogonal subdictionaries to the patch instead of selecting only the nearest one as in previous methods. Extensive experiments on image super-resolution validate the effectiveness and state-of-the-art performance of the proposed method. Additional experiments on contaminated and uncorrelated external data also demonstrate its superior robustness.
Rapid Adsorption of Crystal Violet onto Magnetic Zeolite Synthesized from Fly Ash and Magnetite Nanoparticles

This work reports the adsorption of crystal violet (CV) dye onto magnetic zeolite (MZ) nanoparticles, synthesized by direct fusion of fly ash (FA) and magnetite particles. The synthesised MZ showed high capacity for CV dye adsorption, removing 95% of the dye at an equilibrium adsorption time of 10 min and 25℃. The effects of adsorbent dosage, dye concentration, and pH, on adsorption were evaluated. Adsorption data were best described by the Langmuir adsorption isotherm (R2 = 0.9986), while the adsorption kinetics was best fitted by the pseudo-second-order kinetic model (R2 = 0.9999). Application of the MZs synthesised from inexpensive resources such as FA could ensure the sustainability and cost effectiveness of treating industrial effluent containing basic dyes, especially effluent from the textile industries.
Community and contraceptive choice in rural Thailand: A case study of Nang Rong

This paper blends quantitative with qualitative data in an investigation of community and contraceptive choice in Nang Rong, Thailand. Specifically, it develops an explanation of 1) method dominance within villages, coupled with 2) marked differences between villages in the popularity of particular methods. The quantitative analysis demonstrates the importance of village location and placement of family planning services for patterns of contraceptive choice. The qualitative data provide a complementary perspective, emphasizing the importance of social as well as physical space and giving particular attention to the structure of conversational networks.
CT-guided interventional procedures without CT fluoroscopy assistance: patient effective dose and absorbed dose considerations.

OBJECTIVE The purpose of this study was to determine patient effective dose (E) and peak absorbed dose to the skin of the patient from various CT-guided interventional procedures performed without CT fluoroscopy assistance. MATERIALS AND METHODS In total, 49 interventions were retrospectively studied: 14 biopsies, 14 radiofrequency ablations, 14 abscess drainages, and seven nephrostomies. CT images were acquired from the department's PACS system and reviewed to record the scan parameters of each slice. Entrance surface dose and E were estimated using the Impactscan database and the related Monte Carlo conversion coefficients. RESULTS Median values of E for biopsies, radiofrequency ablations, abscess drainages, and nephrostomies were 23, 35.3, 16.2, and 11.5 mSv, respectively. Respective ranges were 5.8-46.6, 18.4-57.2, 10.9-31.5, and 5.1-32.7 mSv. The corresponding median values and ranges for the peak absorbed dose were 281, 557, 155, and 145 mGy and 133-982, 147-699, 94-315, and 75-297 mGy. The diagnostic scans obtained before the interventions were responsible for 63%, 33% 40%, and 51% of E, respectively. The largest contribution to the peak absorbed dose was due to positioning of the tissue acquisition biopsy gun in biopsies (48%), the radiofrequency needle in ablations (57%), and the catheter in abscess drainages (41%) and nephrostomies (49%). CONCLUSION For the CT interventions studied, and especially for biopsies and radiofrequency ablations, patient effective doses were considerably high. Maximum peak absorbed dose observed was about 1 Gy, considerably lower than the threshold for deterministic effects (2 Gy).
The Genomic Heterogeneity among Mycobacterium terrae Complex Displayed by Sequencing of 16S rRNA and hsp65 Genes

The species identification within Mycobacterium terrae complex has been known to be very difficult. In this study, the genomic diversity of M. terrae complex with eighteen clinical isolates, which were initially identified as M. terrae complex by phenotypic method, was investigated, including that of three type strains (M. terrae, M. nonchromogenicum, and M. triviale). 16S rRNA and 65‐kDa heat shock protein (hsp65) gene sequences of mycobacteria were determined and aligned with eleven other references for the comparison using similarity search against the GenBank and Ribosomal Database Project II (RDP) databases. 16S rRNA and hsp65 genes of M. terrae complex showed genomic heterogeneity. Amongst the eighteen clinical isolates, nine were identified as M. nonchromogenicum, eight as M. terrae, one as M. mucogenicum with the molecular characteristic of rapid growth. M. nonchromogenicum could be subdivided into three subgroups, while M. terrae could be subdivided into two subgroups using a 5 bp criterion (>1% difference). Seven isolates in two subgroups of M. nonchromogenicum were Mycobacterium sp. strain MCRO 6, which was closely related to M. nonchromogenicum. The hsp65 gene could not differentiate one M. nonchromogenicum from M. avium or one M. terrae from M. intracellulare. The nucleotide sequence analysis of 16S rRNA and hsp65 genes was shown to be useful in identifying the M. terrae complex, but hsp65 was less discriminating than 16S rRNA.
Contractile properties and sarcoplasmic reticulum calcium content in type I and type II skeletal muscle fibres in active aged humans

Muscle weakness in old age is due in large part to an overall loss of skeletal muscle tissue, but it remains uncertain how much also stems from alterations in the properties of the individual muscle fibres. This study examined the contractile properties and amount of stored intracellular calcium in single muscle fibres of Old (70 ± 4 years) and Young (22 ± 3 years) adults. The maximum level of force production (per unit cross‐sectional area) in fast twitch fibres in Old subjects was lower than in Young subjects, and the fibres were also less sensitive to activation by calcium. The amount of calcium stored inside muscle fibres and available to trigger contraction was also lower in both fast‐ and slow‐twitch muscle fibres in the Old subjects. These findings indicate that muscle weakness in old age stems in part from an impaired capacity for force production in the individual muscle fibres.
Moving closer to the audience: watching football on television

This article aims to describe specific practices of television spectators based on recordings of English families and friends while watching football on television. Their conversations and the talk and events on television are transcribed and analysed with interactional sociolinguistic and conversation analytical methodologies. By doing 'watching football on television', the spectators constitute themselves as a community of practice. Their strategies include direct address of the television (i.e. the commentator or one of the protagonists of the game) and signalling of independent knowledge and emotions to construct their identities of football fan and expert. Conflict between these two identities may become instantiated in the talk. At times, the spectators mutually negotiate the participant role 'party to the talk at home' for the television. This is done by furnishing second pair parts to the commentators' adjacency pairs. Also, it includes respecting the commentators' turns. Having spent countless hours watching football on television, the spectators manage to carefully construct their talk around the commentators' so that one single, coherent conversation emerges. The practices show how the participants as watchers strive to become part of the spectacle using the television as a bridge to the game itself.
Insufficiently dehydrated hydrogen bonds as determinants of protein interactions

The prediction of binding sites and the understanding of interfaces associated with protein complexation remains an open problem in molecular biophysics. This work shows that a crucial factor in predicting and rationalizing protein–protein interfaces can be inferred by assessing the extent of intramolecular desolvation of backbone hydrogen bonds in monomeric structures. Our statistical analysis of native structures shows that, in the majority of soluble proteins, most backbone hydrogen bonds are thoroughly wrapped intramolecularly by nonpolar groups except for a few ones. These latter underwrapped hydrogen bonds may be dramatically stabilized by removal of water. This fact implies that packing defects are “sticky” in a way that decisively contributes to determining the binding sites for proteins, as an examination of numerous complexes demonstrates.
Comparison of flow cytometry with the Sysmex XT2000iV automated analyzer for the detection of reticulated platelets in dogs.

BACKGROUND Immature (reticulated) platelets (r-PLT) are not routinely assessed by hematology analyzers, but may be useful in the evaluation of the bone marrow response to thrombocytopenia. OBJECTIVE The aim of this study was to compare the Sysmex XT2000iV hematology analyzer with standard flow cytometry for the determination of r-PLT percentage in dogs. METHODS Blood samples were obtained from 40 healthy dogs, 12 thrombocytopenic dogs, and 6 dogs with normal platelet counts but with disorders associated with increased thrombopoiesis. The percentage of r-PLT was determined with a FACscan flow cytometer (r-PLT[F]) using CD61-phycoerythrin antibody and thiazole orange, and with the PLT-O channel of the Sysmex analyzer (r-PLT[S]). Mean platelet volume, platelet distribution width, and platelet large cell ratio were also determined on the Sysmex. Repeatability (intra-assay precision) and effect of storage were tested for the automated analyzer. RESULTS The reference interval (mean+/-1.96 X SD) for r-PLT(F) was 1.91+/-1.29% (range 0.78-3.68%) and for r-PLT(S) was 0.56+/-0.82% (range 0.11-2.16%). For both flow cytometry and the Sysmex, the patient group had a significantly higher mean percentage of r-PLT compared with the control group (P<.0001, unpaired Student's t-tests). Fair correlation (r=0.71; Spearman's regression analysis) was found for r-PLT results between the 2 methods, and a negative proportional systematic bias of -6.26 was found for the Sysmex (Bland-Altman analysis). Based on receiver operating characteristic curves and a cut-off of > or =0.975%, a sensitivity of 94.7% and a specificity of 85.7% were obtained for detecting r-PLT on the Sysmex, using flow cytometry as the reference method. Blood samples stored at 4 degrees C and 25 degrees C had a significant increase in the percentage of r-PLT after 24 and 48 hours, respectively. CONCLUSIONS The PLT-O channel of the Sysmex XT2000iV is capable of detecting immature platelets in healthy, thrombocytopenic, and non-thrombocytopenic ill dogs.
Hunchback‐independent silencing of late Ubx enhancers by a Polycomb Group Response Element.

Drosophila homeotic genes are kept silent outside of their appropriate expression domains by a repressive chromatin complex formed by the Polycomb Group proteins. In the case of the Ubx gene, it has been proposed that the early repressor HB, binding at enhancers, recruits the Polycomb complex and specifies the domain of repression. We show that some Ubx enhancers are activated after blastoderm. If a Polycomb Response Element (PRE) is combined with such late enhancers, repression of a reporter gene can be established everywhere in the embryo, irrespective of the presence or absence of hunchback protein. If, however, these late enhancers are combined with a Ubx early enhancer, as well as a PRE, repression is established only where the reporter gene was inactive at early stages. These results imply that the Polycomb complex is not dependent on hunchback and suggest that the pattern of silencing reflects rather the state of activity of the gene at the time the Polycomb complex is formed.
Correlating Brain Atrophy With Cognitive Dysfunction, Mood Disturbances, and Personality Disorder in Multiple Sclerosis

Neuropsychological impairment is a common feature of multiple sclerosis. Affected patients often have deficits in informationprocessing speed and memory and exhibit psychopathological states such as depression. A minority of patients have rarer affect/mood disorders such as euphoria sclerotica and pathological laughter/crying. Neuropsychological impairment is a major predictor of low quality of life, unemployment, and caregiver distress. Studies evaluating correlations between neuropsychological impairment and findings on magnetic resonance imaging show that neuropsychological dysfunction is associated with lesion burden and diffuse disease in normalappearing brain tissue. However, measures of tissue atrophy including whole‐brain and central atrophy are especially well correlated with and predictive of cognitive impairment. Moreover, recent studies have shown that conventional measures of brain atrophy explain more variance in neuropsychological dysfunction than do measures of lesion burden. In particular, neuropsychological outcomes correspond highly with linear measures of subcortical atrophy such as ventricle enlargement. Within the domain of emotional dysfunction, both lesion burden and atrophy are related to major depressive disorder. Brain atrophy also predicts euphoria and disinhibition. The preliminary data suggest that although lesion burden primarily predicts depressive disorder, both lesion burden and atrophy predict the presence of euphoria. Euphoria and disinhibition likely represent personality change associated with worsening cognition as the disease progresses. Taken together, this growing body of data on magnetic resonance imaging to neuropsychological correlations supports the clinical relevance and validity of brain atrophy as a measure of disease progression in multiple sclerosis. Continuing research focuses on the possible relationship between measures of regional brain atrophy and cognitive and emotional impairment.
Open arms, conflicted hearts: nurse-practitioner's attitudes towards working with lesbian, gay and bisexual patients.

AIMS AND OBJECTIVES To explore nurse-practitioner's attitudes towards working with lesbian, gay and bisexual patients. BACKGROUND Literature suggests that lesbians, gay men and bisexuals have significant health disparities compared to heterosexuals. Although the reasons are multifactorial, research suggests that attitudes of healthcare providers (HCPs) may be a contributing factor in both accessing and receiving care. There is currently no literature exploring the attitudes of the approximately 300,000 nurse-practitioners in the United States. Thus, nurse-practitioners strengths and challenges in providing care to sexual minorities are unknown. DESIGN As part of a larger study, Corbin & Strauss' grounded theory methodology was used to explore the attitudes towards lesbian, gay and bisexual patients among primary care nurse-practitioners in NYC. METHODS Data were collected via individual semi-structured interviews with nurse-practitioners currently in practice in primary or outpatient care in NYC (n = 19). Data were evaluated using the three-step constant comparison method. RESULTS Nurse-practitioners in this study had varied, often overlapping and sometimes conflicting, attitudes about working with lesbian, gay and bisexual patients. The main theme identified was 'open arms, conflicted hearts' with three major subthemes - feeling at home, struggling to maintain professionalism and finding comfort under the umbrella of diversity. CONCLUSIONS Nurse-practitioner participants in this study had varied attitudes about working with lesbian, gay and bisexual patients, ranging from open, confident and comfortable to ambivalent, cautious and unsure about working with lesbian, gay and bisexual patients generally and specifically regarding the health needs of this population. RELEVANCE TO CLINICAL PRACTICE This study highlights the inadequate didactic and clinical preparation most nurse-practitioners feel they have to care for lesbian, gay and bisexual patients. There is a need for increased education for registered nurses and nurse-practitioners regarding lesbian, gay and bisexual culture, their unique healthcare needs, as well as the role of stigma and marginalisation in caring for vulnerable populations.
Individual differences in the temporal variability of medically unexplained symptom reporting.

THEORY Non-specific or medically unexplained symptoms account for up to 35% of outpatient referrals. In contrast to the literature on affect, little is known about how people frame daily symptoms and how these patterns are influenced by individual differences. METHOD Three fixed occasion diary studies over an 8-day period (one on ill veterans of the Gulf War and two on healthy undergraduates) and a single cross-sectional survey (on ill Gulf War veterans) were conducted. The severity and frequency of daily symptoms were assessed in all daily diary samples, together with the Big 5 personality domains in one of the undergraduate samples and in the survey of veterans. The data were analysed using auto-correlations and hierarchical multivariate linear modelling. RESULTS In all the chronically ill and healthy samples, the data suggested potential cyclical patterns for symptom severity reporting. With regard to the frequency of symptom reporting, the veterans showed a pattern of constant symptom reporting. Finally, the relationship between the reported severity of symptoms was attenuated by intellect and surgency. DISCUSSION There is evidence that daily experiences of symptom severity are framed relative to each other and this relationship is influenced by personality. The practical implications of these findings are discussed.
[Breast cancer in women thirty years old or less].

PURPOSE Breast cancer rarely occurs in very young women, its diagnosis and management could sometimes be difficult. Our aim is to analyse the epidemiological and clinicopathological features of a group of very young women and especially to evaluate the results of therapeutic strategy. METHODS We report a retrospective study conducted at the department of radiotherapy in Alexis-Vautrin Centre, concerning 30 patients aged < or = 30 years in whom a diagnosis of invasive breast carcinoma was made between 1986 and 2001. RESULTS Six patients had familial history of breast cancer. Palpable tumor was found in 90% of cases, the average size was 3.5 cm. Eleven patients presented with stage I, 11 presented with stage II, 6 presented with stage III and 2 presented with stage IV. Five cancers were diagnosed after pregnancy (average tumor size = 5.8 cm). Eleven patients received neoadjuvant chemotherapy and 23 (82%) of 28 operable cases of invasive malignancy underwent breast conservative surgery (BCS). We found an invasive ductal carcinoma with grade III in 13/27 cases and a nodal involvement in a half of cases, 11 patients of 26 had no expression of oestrogen receptor. The average follow-up was 5 years: six patients (20%) recurred locally (all of them were initially treated by BCS), four patients developed a contralateral breast cancer and three developed a second malignancy. Ten patients died of their metastatic disease. The 5-year overall survival rate was 78%. CONCLUSION Our results are consistent with those of the published reports and suggest that very young women with breast cancer have a poorer prognosis compared with the older ones. They should receive, according to their prognostic factors, an appropriate regional, systemic and hormonal therapy.
Antagonistic effect of bacteriocin against urinary catheter associated Pseudomonas aeruginosa biofilm

Context: Pseudomonas aeruginosa is a gram negative opportunistic bacteria causes several infections commonly colonize these devices and developing biofilms. Bacteria in biofilm can be up to 1,000 times more resistant to antibiotics than the same bacteria circulating in a planktonic state. Case Report: A total of 10 isolates of Pseudomonas aeruginosa were isolated from catheter associated urinary tract infections. While carbenicillin was the most effective antibiotic, all isolates developed multidrug resistant. Both crude and purified bacteriocin showed marked inhibition activity against planktonic and biofilm of the highly resistant isolate P. aeruginosa P7. Conclusion: Bacteriocin extracted from a locally isolated L. acidophilus has an anti P. aeruginosa biofilm activity also it can be used as a therapeutic agent after adequate in vivo experimentation.