Miracle-dz/UniFAR-FTUs huggingface dataset:
A Metabolomics Exploration of the Sexual Phase in the Marine Diatom Pseudo-nitzschia multistriata

Pseudo-nitzschia multistriata is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presence of substantial changes occurring at the onset of sexual reproduction. Herein, we have hypothesized that the amount and nature of cellular metabolites varies along the mating process. To capture the metabolome of Pseudo-nitzschia multistriata at different harvesting times in an unbiased manner, we undertook an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry. Using three different extraction steps, the method revealed pronounced differences in the metabolic profiles between control cells in the vegetative phase (MT+ and MT−) and mixed strains of opposite MTs (cross) undergoing sexual reproduction. Of the 2408 high-quality features obtained, 70 known metabolites could be identified based on in-house libraries and online databases; additional 46 features could be classified by molecular networking of tandem mass spectra. The reduction of phytol detected in the cross can be linked to the general downregulation of photosynthesis during sexual reproduction observed elsewhere. Moreover, the role of highly regulated compounds such as 7-dehydrodesmosterol, whose changes in abundance were the highest in the experiment, oleamide, ectoine, or trigonelline is discussed.
On the role of aging in cancer incidence: An interpretation of the prostate cancer anomaly with implications for routine screening

Cross‐sectional age‐incidence patterns for the common male and female cancers in Connecticut were normalized to describe the percentage of total incidence that occurred at each age. The pattern for prostate cancer was atypical—less than the 99% confidence limit from the mean at ages less than 60 and greater than the 99% confidence limit from the mean at ages greater than 75. The available international data on latent carcinoma of the prostate, when normalized as above, resembled the mean male and female cancer patterns in Connecticut and suggested that the atypical behavior of clinical prostate cancer was the result of a lag between the incidence of latent carcinoma and promotion to clinical disease. Confirmation of this suggestion, with the potential to distinguish epidemiologically those latent carcinomas that remain asymptomatic from those that progress to malignancy, awaits routine screening for the latent carcinoma.
Intra‐ and inter‐population diversity at short tandem repeat loci in diverse populations of the world

To study the level of intra‐ and inter‐population variation at hypervariable DNA loci, we have characterized 15 human populations of diverse ethnic and geographic origins at six short tandem repeat loci by using the polymerase chain reaction. Even though the spectrum of allelic variation is quite broad and there are substantial differences in allele frequency distributions among populations, in general, populations within a major racial group show a greater degree of similarity. This observation is reflected in the analysis of gene diversity. When the total diversity is apportioned, the maximum variation becomes attributable to inter‐individual differences within a population; of the variation that is attributable to differences between populations within a racial group and differences between racial groups, the former is smaller than the latter. Separate analysis of gene diversity for each of the major population groups based on geographic and ethnic relationship shows that the total gene diversity is higher for the larger racial groups, namely, African, Caucasian and Mongoloid, than the American Indians and the Pacific Islanders. As expected, a reciprocal relationship between gene diversity and FST levels is observed. Higher values of FST in the American Indian and the Pacific Islanders may reflect smaller population size and a higher level of isolation. An analysis of genetic distance encompassing the populations belonging to the three major racial groups recognizes three distinct clusters – all the populations of African affiliation cluster together, as do the Caucasian affiliated and the Mongoloid groups, in two distinct clusters. Interestingly, three broadly classified cosmopolitan US populations, namely, US White, US Black and US Asian, cluster with their ancestrally related populations. This study dispels some of the concerns regarding the applicability of DNA typing data for forensic use.
Determination of the potency of remifentanil compared with alfentanil using ventilatory depression as the measure of opioid effect.

BACKGROUND Remifentanil is a new opioid with properties similar to other mu-specific agonists. To establish its pharmacologic profile relative to other known opioids, it is important to determine its potency. This study investigated the relative potency of remifentanil compared with alfentanil. METHODS Thirty young healthy males were administered double-blind remifentanil or alfentanil intravenously for 180 min using a computer-assisted continuous infusion device. Depression of ventilation was assessed by the minute ventilatory response to 7.5% CO2 administered via a "bag in the box" system. The target concentration of the study drug was adjusted to obtain 40-70% depression of baseline minute ventilation. Multiple blood samples were obtained during and following the infusion. The concentration-effect relationship of each drug was modeled, and the concentration needed to provide a 50% depression of ventilation (EC50) was determined. RESULTS Only 11 subjects in each drug group completed the study; however, there were sufficient data in 28 volunteers to model their EC50 values. The EC50 (mean and 95% confidence interval) for depression of minute ventilation with remifentanil was 1.17 (0.85-1.49) ng/ml and the EC50 for alfentanil was 49.4 (32.4-66.5) ng/ml. CONCLUSION Based on depression of the minute ventilatory response to 7.5% CO2, remifentanil is approximately 40 (26-65) times more potent than alfentanil when remifentanil and alfentanil whole-blood concentrations are compared. As alfentanil is usually measured as a plasma concentration, remifentanil is approximately 70 (41-104) times more potent than alfentanil when remifentanil whole-blood concentration is compared with alfentanil plasma concentration. This information should be used when performing comparative studies between remifentanil and other opioids.
Effects of Prior Use, Intention, and Habit on IT Continuance Across Sporadic Use and Frequent Use Conditions

This article is motivated by the desire to integrate and expand two literature streams, one that models effects of prior information technology (IT) use and habit strength on continued IT use and another that studies how to apply such models to IT that are used in a characteristically sporadic manner. We find that joint predictions of continuance intention, prior IT use, and habit strength within our research model are superior to subsets of the model across the extended range of usage frequency we studied. However, subsets of the model can also provide reasonable predictions where all measures are not available.
Screening chromosomal aberrations by array comparative genomic hybridization in 80 patients with congenital hypothyroidism and thyroid dysgenesis.

OBJECTIVE Congenital hypothyroidism occurs in 1:3500 live births and is therefore the most common congenital endocrine disorder. A spectrum of defective thyroid morphology, termed thyroid dysgenesis (TD), represents 80% of permanent congenital hypothyroidism cases. Although several candidate genes have been implicated in thyroid development, comprehensive screens failed to detect mutation carriers in a significant number of patients with nonsyndromic TD. Due to the sporadic occurrence of TD, de novo chromosomal rearrangements are conceivably representing one of the molecular mechanisms participating in its etiology. METHODS The introduction of array comparative genomic hybridization (CGH) has provided the ability to map DNA copy number variations (CNVs) genome wide with high resolution. We performed an array CGH screen of 80 TD patients to determine the role of CNVs in the etiology of the disease. RESULTS We identified novel CNVs that have not been described as frequent variations in the healthy population in 8.75% of all patients. These CNVs exclusively affected patients with athyreosis or thyroid hypoplasia and were nonrecurrent, and the regions flanking the CNVs were not enriched for segmental duplications. CONCLUSIONS The high rate of chromosomal changes in TD argues for an involvement of CNVs in the etiology of this disease. Yet the lack of recurrent aberrations suggests that the genetic causes of TD are heterogenous and not restricted to specific genomic hot spots. Thus, future studies may have to shift the focus from singling out specific genes to the identification of deregulated pathways as the underlying cause of the disease.
Five-Year Survival Is Similar in Thyroid Cancer Patients with Distant Metastases Prepared for Radioactive Iodine Therapy with either Thyroid Hormone Withdrawal or Recombinant Human TSH

Abstract Context: Elevated levels of TSH markedly enhance the effectiveness of radioiodine (RAI) therapy in metastatic thyroid cancer. Objective: The objective of the study was to compare short-term overall survival in thyroid cancer patients with RAI-avid distant metastases prepared for RAI therapy with either traditional thyroid hormone withdrawal (THW) or recombinant human TSH (rhTSH) stimulation. Design: This was a retrospective chart review. Setting: The study was conducted at a tertiary care comprehensive cancer center. Patients: Patients included 175 patients with RAI avid metastatic disease to lung and/or bone. Interventions: In 58 patients, all RAI treatments (remnant ablation and therapy of metastatic disease) were done with rhTSH stimulation. In 35 patients, all RAI treatments were done after THW. In 82 patients, THW was used for initial RAI treatment(s) with subsequent administered activities given after rhTSH stimulation. Main Outcome Measure: Overall survival was measured. Results: After a median follow-up of 5.5 yr, there were no significant differences in overall survival between patients prepared for RAI therapy with rhTSH alone, THW alone, or THW followed by rhTSH (Kaplan-Meier analysis,P = 0.80). In a multivariate analysis that included clinicopathological features and method of preparation (rhTSH or TWH), only age at diagnosis was an independent predictor of overall survival. Conclusions: Preparation for RAI therapy using either THW or rhTSH stimulation was associated with similar 5-yr overall survival rates in patients with RAI avid thyroid cancer metastases to lung or bone.
Prediction scoring system based on clinicohematologic parameters for cervical cancer patients undergoing chemoradiation

Objective A scoring system based on clinicohematologic parameters in cervical cancer patients receiving chemoradiation has not been reported to date. The aim of this study was to determine the prognostic value of clinicohematologic parameters in patients with cervical cancer undergoing chemoradiation and to develop a prediction scoring system based on these results. Methods A total of 107 patients who received definitive chemoradiation for cervical cancer were enrolled in this study. The clinical data and hematologic parameters were retrospectively reviewed, and their prognostic value in predicting survival was analyzed. The neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-to-monocyte ratio (LMR) and the changes in these hematologic parameters (ΔNLR, ΔPLR, and ΔLMR) between pre- and post-treatment were calculated to determine the specific value of these parameters for predicting patient survival. Results The median follow-up time was 39.9 (range 2.7–114.6) months. The 3-year overall survival rate and progression-free survival rate were 80.9% (95% CI 72.7 to 90.0) and 53.4% (95% CI 44.1 to 64.8), respectively. The median progression-free survival was 67.5 months and the median overall survival was not reached. According to multivariable analysis, a ΔNLR≥0 was significantly associated with decreased progression-free survival (HR=2.91, 95% CI 1.43 to 5.94) and overall survival (HR=3.13, 95% CI 1.18 to 8.27). In addition, age (age <58.5 years; progression-free survival: HR=2.55, 95% CI 1.38 to 4.70; overall survival: HR=4.49, 95% CI 1.78 to 11.33) and the International Federation of Gynecology and Obstetrics (FIGO) stage (Ⅲ-Ⅳ; progression-free survival: HR=2.49, 95% CI 1.40 to 4.43; overall survival: HR=3.02, 95% CI 1.32 to 6.90) were identified as predictors of poor survival. Conclusions Both the age and FIGO stage, as clinical parameters, and the ΔNLR, as a hematologic parameter, were independent prognostic factors for survival for cervical cancer patients treated with chemoradiation. Based on these results, we developed a risk score-based classification system for predicting survival.
Approximation of aging effect on dielectric tissue properties for SAR assessment of mobile telephones

In electromagnetic dosimetry of children heads for mobile telephones, the dielectric properties of biological tissues for adults are so far being used due to the lack of the ones of children. In this paper, we derived an empirical formula according to Lichtenecker's exponential law for the complex permittivity of various tissues as a function of the hydrated rate or the total body water (TBW). We first examined its validity using the data measured by Peyman et al. for rats, and then applied the formula to the dielectric properties of 7-year-old and 3-year-old child head models by means of the relationship between the TBW and the age. With the dielectric properties for children derived in such an approach, we analyzed numerically the spatial peak specific absorption rate (SAR) for a 900-MHz mobile telephone in adult and child head models. As a result, we found that the dielectric properties for children do not affect significantly the 1- or 10- g averaged spatial peak SAR as well as the penetration depth. The finding could be qualitatively explained as cancellation of the increased conductivity and decreased electric field penetrating into the tissue because of the same degree of increase between the conductivity and permittivity in children compared to adults. Even in an extreme case, the age effect on the spatial peak SAR of dielectric properties is still within 10%.
Alvimopan, a selective peripherally acting μ‐opioid antagonist *

Abstract Alvimopan is a novel, peripherally acting μ‐opioid antagonist that is being developed for the management of acute postoperative ileus and for the reversal of the delayed gastrointestinal and colonic transit that result in symptoms such as constipation, nausea and motility disorders in patients treated with opiate analgesics. There is a clinical need for effective medications for the treatment of postoperative ileus and opiate‐induced constipation and other motility disorders. This review addresses the basic and applied pharmacology and current evidence for the use of the medication, alvimopan, in clinical gastroenterology.
Probabilistic Small-Cell Caching: Performance Analysis and Optimization

Small-cell caching utilizes the embedded storage of small-cell base stations (SBSs) to store popular contents for the sake of reducing duplicated content transmissions in networks and for offloading the data traffic from macrocell base stations to SBSs. In this paper, we study a probabilistic small-cell caching strategy, where each SBS caches a subset of contents with a specific caching probability. We consider two kinds of network architectures: 1) The SBSs are always active, which is referred to as the always-on architecture; and 2) the SBSs are activated on demand by mobile users (MUs), which is referred to as the dynamic on–off architecture. We focus our attention on the probability that MUs can successfully download content from the storage of SBSs. First, we derive theoretical results of this successful download probability (SDP) using stochastic geometry theory. Then, we investigate the impact of the SBS parameters, such as the transmission power and deployment intensity on the SDP. Furthermore, we optimize the caching probabilities by maximizing the SDP based on our stochastic geometry analysis. The intrinsic amalgamation of optimization theory and stochastic geometry based analysis leads to our optimal caching strategy, characterized by the resultant closed-form expressions. Our results show that in the always-on architecture, the optimal caching probabilities solely depend on the content request probabilities, while in the dynamic on–off architecture, they also relate to the MU-to-SBS intensity ratio. Interestingly, in both architectures, the optimal caching probabilities are linear functions of the square root of the content request probabilities. Monte-Carlo simulations validate our theoretical analysis and show that the proposed schemes relying on the optimal caching probabilities are capable of achieving substantial SDP improvement, compared with the benchmark schemes.
Regulation of natural cytotoxicity receptors by heparan sulfate proteoglycans in ‐cis: A lesson from NKp44

NKp44 (NCR2) is a distinct member of natural cytotoxicity receptors (NCRs) family that can induce cytokine production and cytolytic activity in human NK cells. Heparan sulfate proteoglycans (HSPGs) are differentially expressed in various normal and cancerous tissues. HSPGs were reported to serve as ligands/co‐ligands for NKp44 and other NCRs. However, HSPG expression is not restricted to either group and can be found also in NK cells. Our current study reveals that NKp44 function can be modulated through interactions with HSPGs on NK cells themselves in ‐cis rather than on target cells in ‐trans. The intimate interaction of NKp44 and the NK cell‐associated HSPG syndecan‐4 (SDC4) in –cis can directly regulate membrane distribution of NKp44 and constitutively dampens the triggering of the receptor. We further demonstrate, that the disruption of NKp44 and SDC4 interaction releases the receptor to engage with its ligands in –trans and therefore enhances NKp44 activation potential and NK cell functional response.
Estimating Chlamydia Screening Rates by Using Reported Sexually Transmitted Disease Tests for Sexually Active Women Aged 16 to 25 Years in the United States

Objective: The objective of this study was to estimate Chlamydia trachomatis (CT) screening rates by using reported sexually transmitted disease (STD) tests for sexually active women aged 16 to 25 years in the U.S. general population. Methods: We analyzed data from the 2002 National Survey of Family Growth. Women were classified as sexually active if they reported having at least one male sex partner in the 12 months before the interview date. Women were classified as tested if they reported being tested for STDs by a healthcare provider in the preceding 12 months. Results: Of 2,563 sampled women aged 16 to 25 years, 75% were estimated to be sexually active. Of sexually active women, 42% reported they had been tested for STDs and 73% reported they had received Pap smears or pelvic examinations in the preceding 12 months. Conclusions: Even if all women tested for STDs were screened for CT, only 42% of sexually active women aged 16 to 25 years would have been screened for CT. CT screening rates could be significantly increased if CT tests were performed when women had Pap smears or pelvic examinations, because most sexually active women have routine Pap smears or pelvic examinations.
Prevalence and distribution of oral lesions: a cross-sectional study in Turkey.

OBJECTIVES The purpose of this study was to evaluate the prevalence and distribution of oral lesions (OLs) in Turkish population. SUBJECTS AND METHODS In this cross-sectional study, 765 residents (F/M: 375/390) were selected by the cluster sampling method and examined according to WHO criteria. RESULTS Excessive melanin pigmentation (6.9%) was the most common lesion in the study population. The tongue lesions observed in this study were as follows: fissured tongue (5.2%), varices (4.1%), hairy tongue (3.8%), geographic tongue (1.0%), atrophic tongue papillae (0.7%) and ankyloglossia (0.3%). The denture-related lesions were denture stomatitis (4.3%), suction irritation (0.8%), denture hyperplasia and torus palatinus as bony lesion (0.5%) and traumatic ulcers (0.3%). In regression analysis, being 65 years old and over was found as a statistically significant risk factor for the occurrence of pigmentation, fissured tongue, varices, hairy tongue, denture stomatitis and petechiae (P < 0.05). CONCLUSIONS Pigmentation, fissured tongue and denture stomatitis were observed to be the most common lesions in Turkish population. Elderly population was a significant risk factor for occurrence of some OLs.
Individualized Supplementation of Folic Acid According to Polymorphisms of Methylenetetrahydrofolate Reductase (MTHFR), Methionine Synthase Reductase (MTRR) Reduced Pregnant Complications

Objective: This study aimed to detect the genotype distributions and allele frequencies of methylenetetrahydrofolate reductase (MTHFR) C677T, A1298C and methionine synthase reductase (MTRR) A66G polymorphisms of pregnant women in Jiaodong region in China, and to investigate whether folic acid supplementation affect the pregnancy complications. Setting: A total of 7,812 pregnant women from the Jiaodong region in Shandong province in China. Methods: By using Taqman-MGB, 2,928 pregnant women (case group) were tested for the genotype distributions and allele frequencies of MTHFR C677T, A1298C and MTRR A66G polymorphisms. Folic acid metabolism ability was ranked at four levels and then pregnant women in different rank group were supplemented with different doses of folic acid. Their pregnancy complications were followed up and compared with 4,884 pregnant women without folic acid supplementation (control group) in the same hospital. Results: The allele frequencies of MTHFR C677T were 49.1 and 50.9%; those of MTHFR A1298C were 80.2 and 19.8%, and those of MTRR A66G were 74.1 and 25.9%. After supplemented with folic acid, the complication rates in different age groups were significantly reduced, especially for gestational diabetes mellitus and hypertension. Conclusion: Periconceptional folic acid supplementation and healthcare following gene polymorphism testing may be a powerful measure to decrease congenital malformations.
Frequency tuning properties of ocular vestibular evoked myogenic potentials

Loud air-conducted sound or bone-conducted vibration produces vestibular-dependent electromyographic responses both in the cervical muscles (cervical vestibular evoked myogenic potentials) and in the extraocular muscles (ocular vestibular evoked myogenic potentials; oVEMPs). Although previous frequency tuning measurements of cervical vestibular evoked myogenic potentials have provided important information for a clinical application, those of oVEMPs have rarely been studied. We explored the frequency tuning properties of oVEMPs in 12 healthy participants. Our results indicate that the best frequencies of the oVEMPs to air-conducted sound and bone-conducted vibration are 500 and 250 Hz, respectively. The difference in the best frequencies between the two stimuli may originate from the difference in the end organs (saccule or utricle), preferentially activated by each stimulus.