Miracle-dz/UniFAR-FTUs huggingface dataset:
Yeasts from macroalgae and lichens that inhabit the South Shetland Islands, Antarctica.

Antarctic terrestrial ecosystems are largely dominated by lichens, while shallow coastal environments are mainly covered by macroalgae. The aim of this study was to isolate and to evaluate the diversity of yeasts in different species of macroalgae and lichens collected in South Shetland Islands, Antarctica. A total of 405 yeasts were recovered (205 from macroalgae and 200 from lichens). The yeast community from macroalgae was most diversity than the yeast community from lichen. The dominance index was similar for both substrates. A total of 24 taxa from macroalgae and 18 from lichens were identified, and only 5 were common to both substrates. Metschnikowia australis, Mrakia sp. , Rhodotorula glacialis and Glaciozyma litorale were the most abundant yeasts in macroalgae and Cryptococcus victoriae, Rhodotorula laryngis, Rhodotorula arctica, Trichosporon sp. 1 and Mrakia sp. were the most abundant in lichens. Based on molecular and phylogenetic analyses, four yeast from macroalgae and six from lichens were considered potential new species. This is the first study to report the yeast communities from the Antarctic macroalgae Himantothallus grandifolius and lichen Ramalina terebrata. Results suggest that Antarctic phyco and lichensphere represent a huge substrate for cold-adapted yeasts and enhanced the knowledge of the microbiota from extreme environments.
Accumulation of classical monocytes defines a subgroup of MDS that frequently evolves into CMML.

To the editor: Even though the diagnosis criteria of chronic myelomonocytic leukemia (CMML) have been recently revised by the World Health Organization (WHO),[1][1] recognition of this disease can be challenging. We demonstrated recently that a percentage of classical monocytes CD14++CD16− (MO1
Parenting Challenges and Needs for Fathers Following Acquired Brain Injury (ABI) in Queensland, Australia: A Preliminary Model

Parenting following acquired brain injury (ABI) has received little empirical or clinical investigation. The current study aimed to explore the challenges facing fathers following ABI and identify their needs for support. Interviews were conducted with three men with ABI, two partners and three rehabilitation coordinators. Five themes emerged from the data and a preliminary model of parenting for fathers following injury was developed. The interactive effects of the ABI and associated impairment on family relationships/functioning, perceptions/identity of parenting role, parenting knowledge/skills and confidence/self efficacy are described. Interventions should be flexible, individualised and tailored to address the unique parenting needs of the father within each family.
Five-year results of brentuximab vedotin in patients with relapsed or refractory systemic anaplastic large cell lymphoma.

This pivotal phase 2 study evaluated the safety and efficacy of brentuximab vedotin in patients with relapsed or refractory (R/R) systemic anaplastic large cell lymphoma (ALCL). After a median observation period of approximately 6 years from first treatment, we examined the durability of remission, progression-free survival (PFS), overall survival (OS), and safety outcomes of patients treated on this trial. Among all enrolled patients (n = 58), no progressions were observed beyond 40 months, and median OS was not reached. Patients with a complete response (CR), as assessed by the investigator (38 of 58, 66%), continued to demonstrate improved outcomes with neither median OS nor PFS reached. Of the 38 CR patients, 16 received a consolidative stem cell transplant (SCT) with median PFS not reached. Among patients who were on-study and in remission at study closure, 16 patients had not received any new treatment after single-agent brentuximab vedotin other than consolidative SCT. Among this subset of 16 patients, 8 received SCT, and the remaining 8 patients (14% of all enrolled patients) remained in sustained remission without consolidative SCT or any new anticancer therapy. Thirty-three patients experienced peripheral neuropathy, among whom, the majority (30 of 33, 91%) had experienced resolution or improvement at their last assessment. These final results, which demonstrated a high rate of peripheral neuropathy resolution, and durable remissions in a subset of patients with relapsed or refractory systemic ALCL, provide evidence that single-agent brentuximab vedotin may be a potentially curative treatment option. This trial was registered at www.clinicaltrials. gov as #NCT00866047.
Eculizumab treatment and impaired opsonophagocytic killing of meningococci by whole blood from immunized adults.

Eculizumab, a humanized anti-complement C5 monoclonal antibody (mAb) for treatment of paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome, blocks the terminal complement pathway required for serum bactericidal activity (SBA). Because treated patients are at >1000-fold increased risk of meningococcal disease, vaccination is recommended; whether vaccination can protect by opsonophagocytic activity in the absence of SBA is not known. Meningococci were added to anticoagulated blood from 12 healthy adults vaccinated with meningococcal serogroup B and serogroup A, C, W, Y vaccines. Bacterial survival was measured after 3-hour incubation in the presence of eculizumab or control complement factor D inhibitor ACH-4471, which blocks the complement alternative pathway (AP) and is in phase 2 development for treatment of PNH. In the absence of inhibitors, colony formation units (CFUs) per milliliter in blood from all 12 immunized subjects decreased from ∼4000 at time 0 to sterile cultures at 3 hours. In the presence of eculizumab, there was a >22-fold increase in geometric mean CFUs per milliliter (90 596 and 114 683 CFU/mL for serogroup B and C strains, respectively; P < .0001 compared with time 0). In the presence of ACH-4471, there was a >12-fold decrease (23 and 331 CFU/mL, respectively; P < .0001). The lack of meningococci killing by blood containing eculizumab resulted from inhibition of release of C5a, a C5 split product needed for upregulation of phagocytosis. The results provide an explanation for the large number of cases of meningococcal disease in immunized patients being treated with eculizumab and suggest that vaccination may provide better protection against meningococcal disease in patients treated with an AP-specific inhibitor.
Vitamin D receptor gene polymorphism: association with Crohn's disease susceptibility

BACKGROUND The vitamin D receptor (VDR) gene represents a strong positional candidate susceptibility gene for inflammatory bowel disease (IBD). The VDR gene maps to a region on chromosome 12 that has been shown to be linked to IBD by genome screening techniques. It is the cellular receptor for 1,25(OH)2 vitamin D3 (calcitriol) which has a wide range of different regulatory effects on the immune system. IBD is characterised by activation of the mucosal immune system. AIM To determine if polymorphisms in the VDR gene are associated with susceptibility to IBD SUBJECTS European Caucasoids: 158 patients with ulcerative colitis, 245 with Crohn's disease, and 164 cadaveric renal allograft donor controls. METHOD Single nucleotide polymorphisms (TaqI,ApaI, and FokI) in VDR were typed in patients with Crohn's disease, ulcerative colitis, and controls by polymerase chain reaction with sequence specific primers. RESULTS There were significantly more homozygotes for the TaqI polymorphism at codon 352 of exon 8 (genotype “tt”) among patients with Crohn's disease (frequency 0.22) than patients with ulcerative colitis (0.12) or controls (0.12) (odds ratio 1.99; 95% confidence interval 1.14–3.47; p=0.017). CONCLUSION This study provides preliminary evidence for a genetic association between Crohn's disease susceptibility and a gene that lies within one of the candidate regions determined by linkage analysis.
Feeding outcomes in preterm infants after discharge from the neonatal intensive care unit (NICU): a systematic review

Abstract Optimal growth and successful feeding in the neonatal intensive care unit (NICU) are difficult to achieve, and data indicate premature infants continue to struggle after discharge. The purpose of this systematic review was to identify growth and feeding outcomes in the NICU published within the last 10 years. Available evidence suggests weight-for-age decreases between birth and discharge from the NICU, and continues to lag behind expectations after discharge. Prevalence rates of breastfeeding differ across countries, with declining rates after discharge from the NICU. Interventions focused on increasing breastfeeding rates are effective. Most healthy preterm infants successfully nipple feed at a gestational age ≥ 36 weeks, but infants may be discharged prior to achieving full oral feeding, or eating with poor coordination. Earlier born preterm infants are later at achieving full oral feedings. After discharge, preterm infants are slower to develop eating skills, parental reports of feeding problems are prevalent, and parents introduce solids to their infants earlier than recommended. This review enhances professionals’ understanding of the difficulties of feeding and growth in preterm born infants that are faced by parents.
Incidência e fatores de risco de lesões em jogadores de futsal portugueses

INTRODUCTION: The reduced number of studies published on the occurrence of injuries in futsal in Portugal motivated this study. OBJECTIVE: This study aimed to identify potential causes for injuries in this sport, which can be a reference for the designing of specific protocols for injury prevention. METHODS: The sample was composed of 411 Portuguese male and female futsal players, of diverse competitive levels. All the participants filled out a previously validated questionnaire, based on retrospective information. Statistical inference consisted of inferential analysis using the Kruskal-Wallis test and the Mann-Whitney test for nonparametric data (significance level of 5%). RESULTS: The results confirm the ankle sprain injury as the injury with the highest incidence (48.8% of total) in futsal. The injuries with recovery period between 8 and 28 days were the most frequent (52.7% of total). This study did not show gender or the position of the player on the field to cause significant differences on the frequency of the injuries, or on the type or anatomical region of the injury. However, there were significant differences between training and competition, with greater occurrence of sprains and contractures in the case of training and higher incidence of muscle tears and fractures in competition, with a longer recovery period resulting from injuries suffered during competition. Moroever, significant differences were found for the mechanism of injury, with higher incidence of bone or joint injuries, sprains and fractures as a result of contact with opponents and higher incidence of muscle or ligament injuries without contact with opponents. The results did not show any significant differences in the laterality of injuries. CONCLUSION: The results highlight the importance of specific programmes for ankle sprain injury prevention, specially in early stages, regardless of their position on the field of play, especially in situations of contact with opponents.
Primary isolated gingival tuberculosis: A rare case report

Tuberculosis is an inflammatory granulomatous systemic disease that rarely presents as primary lesions in gingiva. Gingival involvement has been reported in only a very limited number of cases. A case of primary tuberculosis affecting gingiva and alveolar mucosa is presented. A 20-year-old female patient presented with multiple gingival ulcers, necrotic slough over the gingiva in maxillary arch facially and palatally, and bone destruction. The patient had no evidence of tuberculosis elsewhere. The diagnosis was made following a gingival biopsy. An incisional biopsy with polymerase chain reaction (PCR) and Ziehl Nielsen (ZN) staining was consistent with the diagnosis of gingival tuberculosis. The patient was subsequently referred to the physician for clinical management and the condition resolved with active antitubercular treatment. It is concluded that tuberculosis is a systemic disease that rarely affects the gingiva. The possibility of gingival involvement as sole manifestation of the disease should be considered in the differential diagnosis of gingival lesions
Differential mitochondrial proteome analysis of human lung adenocarcinoma and normal bronchial epithelium cell lines using quantitative mass spectrometry

Lung cancer is one of the higher incidences of malignant tumors around the world. At present, tumor markers CEA, CA19‐9, and CA‐125 in serum are used for the diagnosis of lung cancer, however, fewer studies have shown tumor markers for early diagnosis. Therefore, using quantitative mass spectrometry, differential mitochondrial proteome analysis was performed, comparing human lung adenocarcinoma and normal bronchial epithelium cells.
A Comparison of Problem-Based Learning and Standard Curriculum Students: Three Years of Retrospective Data

Background: Many, many studies, including three major reviews, that compare problem-based curricula to more traditional curricula, have been published in the last 10 years. No study, however, has looked specifically at a closed-loop or reiterative problem-based approach. Purpose: A 3-year retrospective study was done to compare the academic performance of students in a reiterative problem-based learning (PBL)curriculum with that of their counterparts in the standard curriculum to ensure that the PBL students were not disadvantaged. Methods: Three graduating classes' performance on a number of outcome measures, including the United States Medical Licensing Examination, clinical clerkship, and clinical practice examination performance, were compared. Results: Analyses indicate that students in the PBL curriculum performed at least as well as, and in some instances better than, their counterparts in the standard curriculum. Conclusion: Students in the PBL curriculum, a student-directed curriculum, were not d.
Inhibition of carbachol stimulated acid secretion by interleukin 1β in rabbit parietal cells requires protein kinase C

BACKGROUND Interleukin 1β (IL-1β) is a potent inhibitor of gastric acid secretion. Regulatory actions at several levels have previously been demonstrated, including direct inhibition of parietal cell acid secretion. Although IL-1β may activate several intracellular signalling pathways, the mechanisms responsible for inhibition of carbachol stimulated acid secretion have not been determined. AIMS To investigate the roles of protein kinase C (PKC) and the sphingomyelinase signalling pathways in the regulation of acid secretion by IL-1β. METHODS Rabbit parietal cells were obtained by collagenase-EDTA digestion and centrifugal elutriation. Acid secretion stimulated by carbachol andA23187 (to mimic elevations in intracellular calcium) was assessed by14C aminopyrine uptake in response to IL-1β, PKC, and sphingomyelinase manipulation. RESULTS IL-1β inhibited carbachol and A23187 stimulated acid secretion in a dose dependent manner. The inhibitory actions were completely reversed by each of three different PKC inhibitors, staurosporine, H-7, and chelerythrine, as well as by PKC depletion with high dose phorbol ester pretreatment. IL-1β did not downregulate parietal cell muscarinic receptor. IL-1β significantly increased membrane PKC activity. Activation of the sphingomyelinase/ceramide pathway had no effect on basal or stimulated acid secretion. The inhibitory action of IL-1β was independent of protein kinase A and protein kinase G activity. CONCLUSIONS IL-1β directly inhibits parietal cell carbachol stimulated acid secretion. This action occurs distal to muscarinic receptor activation and elevations in intracellular calcium and requires PKC.
Pretreatment neutrophil‐lymphocyte ratio is not a significant prognostic factor in epidermal growth factor receptor‐mutant non‐small cell lung cancer patients treated with tyrosine kinase inhibitors

The neutrophil‐lymphocyte ratio (NLR) is a marker of poor prognosis in lung cancer patients. However, previous data have been based on an heterogeneous population of lung cancer patients and various treatments. In this study, we evaluate the prognostic value of NLR in an homogeneous population of epidermal growth factor receptor (EGFR)‐mutant non‐small cell lung cancer (NSCLC) patients.
Time-redundant multiple computation for fault-tolerant digital neural networks

In mission-critical applications of artificial neural networks, error correction at the architectural level is often mandatory to guarantee consistency and reliability of the network's outputs. Time redundancy allows for fault tolerance with low circuit complexity overhead. In this paper, the application REcomputing with Triplication With Voting (RETWV) at the system level is proposed for concurrent error correction in neural networks. Feed-forward multi-layered neural networks are considered as an example, but the proposed technique can be easily extended to different neural paradigms.
Treatment of intestinal Behçet's syndrome with chimeric tumour necrosis factor α antibody

Few patients with Behçet's syndrome have gastrointestinal ulceration. Such patients are difficult to treat and have a higher mortality. Faced with refractory symptoms in two patients with intestinal Behçet's, we used the tumour necrosis factor α (TNF-α) monoclonal antibody infliximab to induce remission. Both women (one aged 27 years, the other 30 years) presented with orogenital ulceration, pustular rash, abdominal pain, bloody diarrhoea due to colonic ulceration, weight loss, and synovitis. One had thrombophlebitis, digital vasculitis, perianal fistula, and paracolic abscess; the other had conjunctivitis and an ulcer in the natal cleft. Treatment with prednisolone, methyl prednisolone, and thalidomide in one and prednisolone, colchicine, and cyclosporin in the other was ineffective. After full discussion, infliximab (3 mg/kg, dose reduced because of recent sepsis in one, and 5 mg/kg in the other) was administered. Within 10 days the ulcers healed, with resolution of bloody diarrhoea and all extraintestinal manifestations. A second infusion of infliximab was necessary eight weeks later in one case, followed by sustained (>15 months) remission on low dose thalidomide. Remission was initially sustained for 12 months in the other but thalidomide had to be stopped due to intolerance, and a good response to retreatment lasted only 12 weeks without immunosuppression, before a third infusion. The cause of Behçet's syndrome is unknown but peripheral blood CD45 γδ T cells in Behçet's produce >50-fold more TNF-α than controls when stimulated with phorbol myristate acetate and anti-CD3. Infliximab could have a role for inducing remission in Behçet's syndrome.
Promising efficacy and acceptable safety of venetoclax plus bortezomib and dexamethasone in relapsed/refractory MM.

The antiapoptotic proteins BCL-2 and myeloid cell leukemia sequence 1 (MCL-1) promote multiple myeloma (MM) cell survival. Venetoclax is a selective, orally bioavailable small-molecule BCL-2 inhibitor; bortezomib can indirectly inhibit MCL-1. In preclinical studies, venetoclax enhanced bortezomib activity, suggesting that cotargeting of BCL-2 and MCL-1 could be an effective treatment strategy in myeloma. This phase 1b trial studied patients with relapsed/refractory MM receiving daily venetoclax (50-1200 mg per designated dose cohort; 800 mg in safety expansion) in combination with bortezomib and dexamethasone. A total of 66 patients were enrolled (54 in the dose-escalation cohorts and 12 in the safety expansion). Patients had received a median of 3 prior therapies (range, 1-13); 26 (39%) were refractory to prior bortezomib and 35 (53%) to lenalidomide; 39 (59%) had prior stem cell transplant. The combination was generally well tolerated, and common adverse events included mild gastrointestinal toxicities (diarrhea [46%], constipation [41%], and nausea [38%]) and grade 3/4 cytopenias (thrombocytopenia [29%] and anemia [15%]). The overall response rate (ORR) was 67% (44/66); 42% achieved very good partial response or better (≥VGPR). Median time to progression and duration of response were 9.5 and 9.7 months, respectively. ORR of 97% and ≥VGPR 73% were seen in patients not refractory to bortezomib who had 1 to 3 prior therapies. Patients with high BCL2 expression had a higher ORR (94% [17/18]) than patients with low BCL2 expression (59% [16/27]). This novel combination of venetoclax with bortezomib and dexamethasone has an acceptable safety profile and promising efficacy in patients with relapsed/refractory MM. This trial was registered at www.clinicaltrials. gov as #NCT01794507.
Ultrathin DSAEK vs. Conventional DSAEK: Results from a Dutch multicentre randomised clinical trial

The Dutch Ultra‐Thin DSAEK study is a multicenter randomized controlled study comparing the effectiveness and cost‐effectiveness of precut ultra‐thin (<100 m) DSAEK with standard thickness DSAEK. Fifty‐eight adult patients with Fuchs endothelial dystrophy and no other visual limiting comorbidities were randomized to either ultra‐thin or standard DSAEK in four tertiary medical centers in The Netherlands. Preliminary (6 months follow‐up) outcomes, including: ETDRS best corrected visual acuity, refractive and topographic astigmatism, endothelial cell density, contrast sensitivity, straylight, complication profile, quality of life and vision related quality of life will be presented.
Effect of low-carbohydrate high-protein diets on acid-base balance, stone-forming propensity, and calcium metabolism

Abstract Background: Low-carbohydrate high-protein (LCHP) diets are used commonly for weight reduction. This study explores the relationship between such diets and acid-base balance, kidney-stone risk, and calcium and bone metabolism. Methods: Ten healthy subjects participated in a metabolic study. Subjects initially consumed their usual non-weight-reducing diet, then a severely carbohydrate-restricted induction diet for 2 weeks, followed by a moderately carbohydrate-restricted maintenance diet for 4 weeks. Results : Urine pH decreased from 6.09 (Usual) to 5.56 (Induction; P P P P P P P P P P N -telopeptide levels trended upward, whereas serum osteocalcin concentrations decreased significantly ( P Conclusion: Consumption of an LCHP diet for 6 weeks delivers a marked acid load to the kidney, increases the risk for stone formation, decreases estimated calcium balance, and may increase the risk for bone loss. © 2002 by the National Kidney Foundation, Inc.