Miracle-dz/UniFAR-FTUs huggingface dataset:
Ionic signalling in astroglia beyond calcium

Astrocytes are homeostatic and protective cells of the central nervous system. Astroglial homeostatic responses are tightly coordinated with neuronal activity. Astrocytes maintain neuronal excitability through regulation of extracellular ion concentrations, as well as assisting and modulating synaptic transmission by uptake and catabolism of major neurotransmitters. Moreover, they support neuronal metabolism and detoxify ammonium and reactive oxygen species. Astroglial homeostatic actions are initiated and controlled by intercellular signalling of ions, including Ca2+, Na+, Cl−, H+ and possibly K+. This review summarises current knowledge on ionic signals mediated by the major monovalent ions, which occur in microdomains, as global events, or as propagating intercellular waves and thereby represent the substrate for astroglial excitability.
THE EFFECTS OF WHOLE BODY VIBRATION ON VERTICAL JUMP, POWER, BALANCE, AND AGILITY IN UNTRAINED ADULTS.

Background Despite the increased use of whole body vibration among athletes, there is limited literature on its acute effects within heterogeneous populations such as untrained adults or recreational athletes. Hypothesis/Purpose The purpose of this study was to investigate the acute effects of whole body vibration on vertical jump, power, balance, and agility for untrained males and females. It was hypothesized that there would be an effect on each outcome variable. Study Design Quasi-experimental, pretest-posttest design. Methods Twenty males and sixteen females, mean age 24.5 years, were assessed for vertical jump height and power as measured by the Myotest accelerometer, balance as measured by the NeuroCom Balance Master System, and agility as measured by a modified T-test. Each session consisted of a five-minute treadmill warm-up, a practice test, a baseline measurement, a two-minute rest period, whole body vibration at 2 mm and 30 Hz for 60 seconds, and a final measurement. Three different counterbalanced testing sessions were separated by a minimum of 48 hours in between sessions to minimize fatigue. Results Significant differences existed for both genders for main effect of time for Agility (p = 0.022); end point excursion Left (p = 0.007); and maximum endpoint excursion Left (p = 0.039). Differences for main effect of gender revealed females performed better than males in the following respects: end point excursion Right (p = 0.035); end point excursion Left (p = 0.014); maximum endpoint excursion Right (p = 0.024); and maximum endpoint excursion Left (p = 0.005). Males performed better than females in two respects: Agility (p < 0.0005) and Power (p < 0.0005). A significant interaction was observed between time and gender for vertical jump (p = 0.020). Simple main effects revealed males jumped higher than females during both pre and post intervention, p < 0.0005. Females had a significant decrease in the vertical jump post intervention (p = 0.05). Conclusion Results indicated that whole body vibration produced significant differences in the main effect of time and agility, and end point and maximum end point excursion Left for both genders, acutely. Females performed better in balance compared to males and poorer in vertical jump, but males performed better in agility and power.
Evolving Trends in Liver Transplant for Metabolic Liver Disease in the United States

Indications for liver transplantation (LT) in metabolic disease are evolving. We reviewed the US experience with primary LT for metabolic disease in the Scientific Registry for Transplant Recipients (October 1987 to June 2017) to determine the following: temporal changes in indications, longterm outcomes, and factors predicting survival. Patients were grouped by the presence of structural liver disease (SLD) and whether the defect was confined to the liver. There were 5996 patients who underwent LT for metabolic disease, 2354 (39.3%) being children. LT for metabolic disease increased in children but not in adults. Children experienced a 6‐fold increase in LT for metabolic disease without SLD. Indications for LT remained stable in adults. Living donor liver transplantation increased between era 1 and era 3 from 5.6% to 7.6% in children and 0% to 4.5% in adults. Patient and graft survival improved with time. The latest 5‐year patient survival rates were 94.5% and 81.5% in children and adults, respectively. Outcomes were worse in adults and in those with extrahepatic disease (P < 0.01), whereas SLD did not affect outcomes. Survival improved with younger age at LT until age <2 years. On multivariate analysis, diagnostic category, inpatient status, age at LT, and transplant era significantly predicted outcomes in all ages with male sex predicting survival in childhood only. Children without structural disease were less likely to die awaiting LT and had improved post‐LT survival compared with children with chronic liver disease. In conclusion, LT for metabolic disease is increasingly used for phenotypic correction in children; extrahepatic manifestations significantly impact survival at all ages; where indicated, transplantation should not be unnecessarily delayed; and the development of new allocation models may be required.
Growth and Enterotoxin Production by Staphylococci in Genoa Salami 1.

Staphylococcus aureus strains 265 and 243 which produce enterotoxins A and B, respectively, were inoculated into meat being made into Genoa salami in the amount of 103, 105, and 107, cells/g. No lactic starter culture was added. Samples were taken at different stages of processing to determine the microbial populations, percentage moisture, total acidity, pH, and enterotoxin content. Staphylococcal populations varying from about 107 to 5 × 108/g were detected during tempering of the salami. Enterotoxin A was detected in surface but not in core samples of salami inoculated with 105 and 107 S. aureus 265 cells/g. However, no enterotoxin B was detected in the salami inoculated with S. aureus 243, which requires a relatively high aw for enterotoxin production. Staphylococcal counts were higher in surface samples than in core samples, attributable to the difference in oxygen, but there was no significant difference in microaerophilic lactic Kid bacteria in different portions of the salami.
Ticagrelor Versus Clopidogrel in Comatose Survivors of Out-of-Hospital Cardiac Arrest Undergoing Percutaneous Coronary Intervention and Hypothermia: A Randomized Study.

Comatose survivors of out-of-hospital cardiac arrest increasingly undergo immediate percutaneous coronary intervention (PCI) and stenting, which is associated with the need for administration of P2Y12 inhibitor.1 Because these patients remain intubated and mechanically ventilated after reestablishment of spontaneous circulation, administration of crushed and dissolved tablets by nasogastric tube remains the only option. Because the antiplatelet effect of clopidogrel is significantly decreased during the first 48 hours after reestablishment of spontaneous circulation,2 we hypothesized that a novel P2Y12 inhibitor, ticagrelor, which does not require metabolic activation in the liver, might result in faster and stronger platelet inhibition. Consecutive comatose survivors of out-of-hospital cardiac arrest with reestablishment of spontaneous circulation undergoing immediate PCI receiving a periprocedural bolus of 250 to 500 mg acetylsalicylic acid (Aspegic, Sanofi-Aventis) and unfractionated heparin to target activated clotting time between 250 and 300 seconds were randomized to ticagrelor (Brilique, AstraZeneca) or clopidogrel (Plavix, Bristol-Myers Squibb) with a random table and sealed envelope system. The tablets were crushed, dissolved into 15 to 20 mL of distilled water, and injected using a nasogastric tube. Ticagrelor was administered as 180 mg loading and then 90 mg every 12 hours. Clopidogrel was given as 600 …
Potential of Osteopontin in the Management of Epithelial Ovarian Cancer

Abstract Background Osteopontin (sOPN) is a promising blood tumour marker for detecting epithelial ovarian cancer (EOC). However, other clinical uses of sOPN as a tumour marker in EOC are still lacking. Since sOPN concentrations in serum are not associated with those in ascites, we compared clinical value of sOPN concentrations in the two body fluids. Patients and methods The study included 31 women with advanced EOC and 34 women with benign gynaecological pathology. In the EOC group, serum for sOPN analysis was obtained preoperatively, after primary debulking surgery and after chemotherapy. In the control group, serum was obtained before and after surgery. Ascites and peritoneal fluid were obtained during surgery. sOPN concentrations were determined by flow cytometry bead-based assay. Results The sensitivity and specificity of sOPN in detecting EOC was 91.2% and 90.3% (cut-off = 47.4 ng/ml) in serum, and 96.8% and 100% (cut-off = 529.5 ng/ml) in ascites. Kaplan-Meier analysis showed a significant association between higher serum sOPN concentration and overall survival (p = 0.018) or progression free survival (p = 0.008). Higher ascites sOPN concentrations were associated with suboptimally debulked tumour and unresectable disease. Higher serum sOPN concentrations were associated with refractory disease or incomplete response to platinum-based chemotherapy. Conclusions The study showed that ascites sOPN level mirrors present disease and is superior to serum level for diagnostic purposes and surgical planning, although the end result of treatment is the response of the whole body in fighting the disease. The preoperative sOPN concentration in serum thus better reflects disease outcome.
Introducing a Crystallography-Mediated Reconstruction (CMR) Approach to Atom Probe Tomography

Abstract Current approaches to reconstruction in atom probe tomography produce results that exhibit substantial distortions throughout the analysis depth. This is largely because of the need to apply a multitude of assumptions when estimating the evolution of the tip shape, and other pseudo-empirical reconstruction factors, which vary both across the face of the tip and throughout the analysis depth. We introduce a new crystallography-mediated reconstruction to improve the spatial accuracy and dramatically reduce these in-depth variations. To achieve this, we developed a barycentric transform to directly relate atomic positions in detector space to real space. This is mediated by novel crystallographic analysis techniques, including: (1) calculating the orientation of a crystal directly from the field evaporation map, (2) tracking pole locations throughout the evaporation sequence, and (3) accounting for the evolving tip radius in a manner that removes the dependence on the geometric field factor. By improving the in-depth spatial accuracy of the atom probe reconstruction, a greater accuracy of the atomic neighborhood relationships is available. This is critical in modern materials science and engineering, where an understanding of the solid solution architecture, precipitate dispersions, and descriptions of the interfaces between phases or grains are key inputs to microstructure–property relationships.
An algebra for composing access control policies

Despite considerable advancements in the area of access control and authorization languages, current approaches to enforcing access control are all based on monolithic and complete specifications. This assumption is too restrictive when access control restrictions to be enforced come from the combination of different policy specifications, each possibly under the control of independent authorities, and where the specifics of some component policies may not even be known apriori. Turning individual specifications into a coherent policy to be fed into the access control system requires a nontrivial combination and translation process. This article addresses the problem of combining authorization specifications that may be independently stated, possibly in different languages and according to different policies. We propose an algebra of security policies together with its formal semantics and illustrate how to formulate complex policies in the algebra and reason about them. A translation of policy expressions into equivalent logic programs is illustrated, which provides the basis for the implementation of the algebra. The algebra's expressiveness is analyzed through a comparison with first-order logic.
Comparison of Standard Methods for Assessing Dietary Intake of Benzo[a]pyrene

Background: Inconsistent presence and strength of associations between dietary benzo[a]pyrene (BaP) exposure and cancers may be due to differences in exposure assessment methods. Thus, we determined correlations of usual meat and BaP intake among three methods: food frequency questionnaires (FFQ), diet diaries, and a biomarker. Methods: Thirty-six nonsmokers were recruited in Baltimore, MD during 2004–2005. Meat and BaP intake estimated from baseline and follow-up FFQs combined with a BaP residue database (FFQ-RD), mean meat and BaP intake estimated from three diet diaries coupled with the residue database (Diary-RD), and mean of three urinary 1-hydroxypyrene glucuronide (1-OHPG) measurements were compared using Spearman correlations. Collections spanned approximately nine months. Results: BaP intakes from meat from the baseline [median = 6.4, interquartile range (IQR) = 13.9 ng/d] and follow-up FFQ-RD (median = 7.3, IQR = 35.7 ng/d) were higher than the Diary-RD (median = 1.1, IQR = 7.4 ng/d). Mean 1-OHPG concentration was weakly correlated with mean meat intake (r = 0.33, P = 0.05) and BaP intake from meat (r = 0.27, P = 0.11) from the Diary-RD. Mean BaP intake estimated from the Diary-RD was positively correlated with the follow-up (r = 0.35, P = 0.04) but not baseline (r = 0.20, P = 0.24) FFQ; the converse was true for meat intake. Conclusions: Diary-RD estimates were supported by biomarker measurements, but considerable unexplained variability remained. Limited correlation among the dietary BaP exposure assessment methods could be due to differences in timeframes covered by the assessments, interpersonal variability in metabolism, deficiencies in the residue database, or nondietary exposures to BaP. Impact: Limited correlation in estimated BaP intake among standard methods may contribute to inconsistent epidemiology of BaP and cancer. Cancer Epidemiol Biomarkers Prev; 20(5); 962–70. ©2011 AACR.
Ego Depletion as a Measure of Emotion Processing Deficits among MUS Patients

Abstract Previous studies have found a link between emotion processing deficits and medically unexplained physical symptoms (MUS). The majority of previous studies have used self-report measures of emotional processing, with the majority focusing on alexithymia, and have produced inconsistent and contradictory results. In the present study, we investigated the role of emotion-processing deficits in MUS by assessing emotion regulation performance and the effects of performance on ego depletion. Participants with high or low levels of self-reported MUS watched emotion-evoking videos under instructions to allow or suppress their emotional reactions, and then performed an anagram task to measure their effort and perseverance. Contrary to the research with self-report measures, experimental analysis of emotion-processing revealed that MUS is correlated with the inability to regulate negative emotions. Unexpected self-regulatory tendencies of the participants with high symptom levels were discussed.
Barriers and Levels of Physical Activity in Patients With Symptomatic Peripheral Artery Disease: Comparison Between Women and Men.

This cross-sectional study compared physical activity levels and barriers between 212 men and women with symptomatic peripheral artery disease. Physical activity was objectively measured by an accelerometer. Barriers to physical activity were obtained using a validated questionnaire. Women reported higher amounts of light physical activity (p < .001) and lower moderate-vigorous physical activity (p < .001) than men. Women more often reported barriers such as "not having anyone to accompany" (p = .006), "lack of money" (p = .018), "fear of falling or worsening the disease" (p = .010), "lack of security" (p = .015), "not having places to sit when feeling leg pain" (p = .021), and "difficulty in getting to a place to practice physical activity" (p = .015). In conclusion, women with symptomatic peripheral artery disease presented with lower amounts of moderate-vigorous activity and more barriers to activity than men. Strategies to minimize the barriers, including group actives and nonpainful exercises, are recommended for women with peripheral artery disease.
Rehabilitation after Hip Fracture for Nursing Home Residents: A Controlled Feasibility Trial.

Background This study compared function three-months after hip fracture surgery between nursing home residents participating in a 10-week outreach rehabilitation program and those receiving usual care. Function, health-related quality of life (HRQL) and mortality were also compared over 12-months and outreach program feasibility was assessed. Methods A feasibility trial was undertaken in Canadian nursing homes; of 77 participants, 46 were allocated to Outreach and 31 to Control prior to assessing function or cognition. Outreach participants received 10 weeks of rehabilitation (30 sessions) and Control participants received usual post-hospital fracture care in their nursing homes. The primary outcome was the Functional Independence Measure Physical Domain(FIMphysical) score three-months post-fracture; we also explored FIM Locomotion and Mobility. Secondary outcomes were FIM scores, EQ-5D-3L scores and mortality over 12-months. Program feasibility was also evaluated. Results The mean age was 88.7±7.0 years, 55(71%) were female and 58(75%) had severe cognitive impairment with no significant group differences(p>0.14). Outreach participants had significantly higher FIM Locomotion than usual care (p=0.02), but no significant group differences were seen in FIMphysical or FIM Mobility score three-months post-fracture. In adjusted analyses, Outreach participants reported significant improvements in all FIM and EQ-5D-3L scores compared to Control participants over 12-months(p<0.05). Mortality did not differ by group(p=0.80). Thirty(65%) outreach participants completed the program. Conclusion Our feasibility trial demonstrated that Outreach participants achieved better locomotion by three-months post-fracture compared to participants receiving usual post-fracture care; benefits were sustained to 12-months post-fracture. In adjusted analyses, Outreach participants also showed sustained benefits in physical function and HRQL.
Systematic review with meta‐analysis: cholecystectomy for biliary dyskinesia—what can the gallbladder ejection fraction tell us?

Gallbladder dyskinesia (gallbladder spasm, biliary dyskinesia or chronic acalculous cholecystitis) is a poorly defined entity which presents as biliary‐type pain without any identifiable organic pathology. Abnormal gallbladder ejection fraction (GBEF) is used by some to select those likely to benefit from cholecystectomy. The validity of this approach has been questioned.
circOMA1-Mediated miR-145-5p Suppresses Tumor Growth of Nonfunctioning Pituitary Adenomas by Targeting TPT1.

CONTEXT Nonfunctioning pituitary adenomas (NFPAs) are the major cause of hypopituitarism and infertility. However, the pathogenesis of NFPAs remains largely unknown. Previous studies have demonstrated the crucial role of miRNAs in the progression of pituitary adenomas. Increasing evidence has indicated that circular RNAs (circRNAs) might mediate miRNA transcriptional activity, providing new insights to study the pathogenesis of NFPAs. OBJECTIVES To explore the regulation and activity of the circRNA-miRNA-mRNA axis in the tumorigenesis of NFPAs. DESIGN The function of miR-145-5p in NFPAs was investigated invitro and invivo. The mechanical details were explored and potential targets of miR-145-5p were identified. Finally, miR-145-5p-associated circRNAs were functionally recognized and confirmed. RESULTS miR-145-5p was markedly decreased in NFPA samples and correlated negatively with NFPA invasiveness. Overexpression of miR-145-5p suppressed NFPA cell proliferation and invasiveness and promoted apoptosis. Further results confirmed that translationally controlled tumor protein (TPT1) is a target of miR-145-5p and mediated the effect of miR-145-5p. TPT1 and its downstream factors Mcl-1 and Bcl-xL were downregulated, and Bax was upregulated by miR-145-5p. Moreover, circOMA1 (hsa_circRNA_0002316) was demonstrated to sponge miR-145-5p, whose suppression on NFPA cells was abrogated by circOMA1 overexpression. circOMA1 silencing exhibited a similar inhibitory effect with miR-145-5p overexpression by downregulating TPT1. We found that circOMA1 could further upregulate Mcl-1 and Bcl-xL and downregulate Bax. CONCLUSIONS circOMA1 promotes NFPA progression by acting as the sponge of tumor suppressor miR-145-5p to regulate the TPT1 signaling pathway, revealing a therapeutic target in preventing the tumorigenesis of NFPAs.
From poly(alkyl cyanoacrylate) to squalene as core material for the design of nanomedicines

Abstract This review article covers the most important steps of the pioneering work of Patrick Couvreur and tries to shed light on his outstanding career that has been a source of inspiration for many decades. His discovery of biodegradable poly(alkyl cyanoacrylate) (PACA) nanoparticles (NPs) has opened large perspectives in nanomedicine. Indeed, NPs made from various types of alkyl cyanoacrylate monomers have been used in different applications, such as the treatment of intracellular infections or the treatment of multidrug resistant hepatocarcinoma. This latest application led to the Phase III clinical trial of Livatag®, a PACA nanoparticulate formulation of doxorubicin. Despite the success of PACA NPs, the development of a novel type of NP with higher drug loadings and lower burst release was tackled by the discovery of squalene-based nanomedicines where the drug is covalently linked to the lipid derivative and the resulting conjugate is self-assembled into NPs. This pioneering work was accompanied by a wide range of novel applications which mainly dealt with the management of unmet medical needs (e.g. pancreatic cancer, brain ischaemia and spinal cord injury).
Incremental utility of an extended stroop task for identifying subtle differences in cognitive performance among healthy older adults

Abstract The Stroop Color and Word Test is a test of processing speed, response inhibition, and executive functioning (EF). This project examined whether extending the Stroop Color-Word trial beyond the standard time limit could more accurately assess performance on EF measures. Cognitively healthy older individuals (n = 198) enrolled in a study of cardiovascular health completed the Stroop as part of a neuropsychological battery. Two scores were computed for the Color-Word trial: the number of items completed within the first 45 seconds (traditional Color-Word score) and the speed of page completion beyond the first 45 seconds (Stroop-Extended score). Criterion measures included the Trail-Making Test Part B (TMT-B), Digit Span Backward, Symbol Digit Modalities Test, Short Category Test, and measures of verbal fluency. Results from hierarchical linear regression analyses indicated that the extended Stroop score accounted for small but statistically significant variance in TMT-B (additional 2.6%) and Digit Span Backwards (additional 2.6%) beyond the standard Color-Word score. These findings suggest that extending the Stroop Color-Word trial beyond the first 45 seconds provides a limited increase in predictive power within a healthy sample with restricted range of performance. The extended Stroop requires additional examination in heterogeneous samples, including clinical populations, to determine its predictive utility.