Miracle-dz/UniFAR-FTUs huggingface dataset:
Witnesses in the consultation room – Experiences of peer group supervision

Abstract Background: In Denmark group supervision for general practitioners is an established part of continuing professional development. Several different approaches are used, including Balint Groups and Bendix Groups. The aim of this paper is to describe the benefits and challenges of a peer group supervision model with the role of supervisor taken by group members in turn. The setting was a group of general practitioners using a patient-centred consultation model and video-recordings of consultations. Methods and material: As part of a larger study the first author studied this long-established group by observing three group sessions over a period of six months. She conducted two individual, semi-structured interviews with each general practitioner who presented consultations at the observed sessions, and held a group interview with the entire group at the end of the observation period. The interviews were recorded digitally and transcribed verbatim. The data were analysed using systematic text condensation. Results and conclusion: Participation seemed to improve communication skills and the ability to take a more patient-centred approach. It increased job satisfaction and prevented burnout. A non-judgemental environment, respect and acknowledgement of all participants were important for developing confidence and for supporting personal and professional development.
Intimate Encounters: Filipina Women and the Remaking of Rural Japan

List of Illustrations Acknowledgments Introduction: Relations of Cultural Production PART ONE Figures of Desires 1. Sites of Encounter 2. America and Other Stories of Filipina Migration to Japan 3. Japan in the Kiso Valley, the Kiso Valley in Japan PART TWO Terms of Relations 4. Kindred Subjects 5. The Pressures of Home 6. Runaway Stories Epilogue Appendix A Appendix B Notes Bibliography Index
On the inherent properties of Soluplus and its application in ibuprofen solid dispersions generated by microwave-quench cooling technology

Abstract Polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer, or Soluplus®, is a relatively new copolymer and a promising carrier of amorphous solid dispersions. Knowledge on the inherent properties of Soluplus® (e.g. cloud points, critical micelle concentrations, and viscosity) in different conditions is relatively inadequate, and the application characteristics of Soluplus®-based solid dispersions made by microwave methods still need to be clarified. In the present investigation, the inherent properties of a Soluplus® carrier, including cloud points, critical micelle concentrations, and viscosity, were explored in different media and in altered conditions. Ibuprofen, a BCS class II non-steroidal anti-inflammatory drug, was selected to develop Soluplus®-based amorphous solid dispersions using the microwave-quench cooling (MQC) method. Scanning electronic microscopy (SEM), differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), Raman spectroscopy (RS), and Fourier transform infrared spectroscopy (FT-IR) were adopted to analyze amorphous properties and molecular interactions in ibuprofen/Soluplus® amorphous solid dispersions generated by MQC. Dissolution, dissolution extension, phase solubility, equilibrium solubility, and supersaturated crystallization inhibiting experiments were performed to elucidate the effects of Soluplus® on ibuprofen in solid dispersions. This research provides valuable information on the inherent properties of Soluplus® and presents a basic understanding of Soluplus® as a carrier of amorphous solid dispersions.
Extracellular Arginine Rapidly Dilates In Vivo Intestinal Arteries and Arterioles Through a Nitric Oxide Mechanism

Objective: Arginine used for nitric oxide formation can be from intracellular stores or transported into cells. The study evaluated the rapidity, and primary site of NO and vascular resistance responses to arginine at near physiological concentrations (100–400 μ M). Methods: Arginine was applied to a single arteriole through a micropipette to determine the fastest possible responses. For vascular blood flow and [NO] responses, arginine was added to the bathing media. Results: Dilation of single arterioles to arginine began in 10–15 seconds and application over the entire vasculature increased [NO] in ∼ 60–90 seconds, and flow increased within 120–300 seconds. Resting periarteriolar [NO] for arterioles was 493.6 ± 30.5 nM and increased to 696.1 ± 68.2 and 820.1 ± 110.5 nM at 200 and 400 μ M L‐arginine. The blood flow increased 50% at 400–1200 μ M L‐arginine. The reduced arterial resistance during topical arginine was significantly greater than microvascular resistance at 100 and 200 μ M arginine. All responses were blocked by L‐NAME. Conclusions: This study demonstrated arterial resistance responses are as or more responsive to arginine induced NO formation as arterioles at near physiological concentrations of arginine. The vascular NO and resistance responses occurred rapidly at L‐arginine concentrations at and below 400 μ M, which predict arginine transport processes were involved.
Uranium and other heavy metal resistance and accumulation in bacteria isolated from uranium mine wastes

Ten bacterial strains isolated from uranium mine wastes were characterized in terms of their uranium and other metal resistance and accumulation. 16S rRNA gene sequence analysis identified the strains as members of genera Bacillus, Serratia, and Arthrobacter. Strains were able to utilize various carbon sources, particularly aromatic hydrocarbons, grow at broad pH and temperature ranges and produce non specific acid phosphatase relevant for metal phosphate precipitation in contaminated environment. The isolates exhibited high uranium and other heavy metals (Ni, Co, Cu and Cd) resistance and accumulation capacities. Particularly, Arthrobacter sp. J001 and Bacillus sp. J003 were superior in terms of U resistance at low pH (pH 4.0) along with metals and actinides (U and Th) removal with maximum cell loading of 1088 μmol U, 1293 μmol Th, 425 μmol Cu, 305 μmol Cd, 377 μmol Zn, 250 μmol Ni g−1 cell dry wt. Genes encoding P1B-type ATPases (Cu-CPx and Zn-CPx) and ABC transporters (nik) as catalytic tools for maintaining cellular metal homeostasis were detected within several Bacillus spp. , with possible incidence of horizontal gene transfer for the later gene showing phylogenetic lineage to α Proteobacteria members. The study provides evidence on intrinsic abilities of indigenous bacteria from U-mine suitable for survival and cleaning up of contaminated mine sites.
Using urinary 1-hydroxypyrene concentrations to evaluate polycyclic aromatic hydrocarbon exposure in women using biomass combustion as main energy source

Abstract Context: The use of solid fuels for cooking and heating is likely to be the largest source of indoor air pollution on a global scale. Objective: The aim of this study was to investigate the urinary excretion of 1-hydroxypyrene (1-OHP) in women living in a rural community, where biomass combustion is used as main energy source during a working day. Materials and methods: The study was performed on urine samples collected in 2012, of 30 healthy women who were residents of a rural community in San Luis Potosi, Mexico. Urine was collected from each woman at three time points (morning, post-lunch and evening) during a working day. The analysis of urinary 1-OHP was performed using high-performance liquid chromatography (HPLC). Also, a health-risk assessment was conducted. Results: The highest levels of 1-OHP in this study were found in the samples taken in the evening (geometric mean ± SD; 0.36 ± 0.13 µg/g creatinine). However, no significant differences among 1-OHP concentrations in the evening and post-lunch samples (0.27 ± 0.10 µg/g creatinine; 0.58 ± 0.67 µg/L) were observed. But, the 1-OHP levels (0.17 ± 0.13 µg/g creatinine; 0.19 ± 0.21 µg/L) found in samples collected in the morning were significantly lower than the 1-OHP levels found in samples collected during post-lunch and evening time. Discussion and conclusion: The data shown in this study demonstrated an increment in the exposure levels to PAHs in women across the shift. However, no health risk was found in this study.
Exploiting organizer influence and geographical preference for new event recommendation

The rapid growth of event‐based social networks (EBSNs) has generated great demand for personalized event recommendation. Indeed, new events are published every day in EBSNs. In this paper, we focus on the problem of recommending new events (i.e., the newly published events that have not yet received any user response) to registered users in EBSNs. Notice that collaborative filtering is ineffective for recommending new events due to the short lifetime of events and the lack of historical information, that is, the cold‐start problem. A straightforward approach to address this problem is to adopt content‐based recommendation techniques, for example, deriving user interests through the content information of the events they attended. Nevertheless, we observe that organizer influence and geographical preference also play important roles in a user's decision to participate in an event. Motivated by this observation, we combine user interest, organizer influence, and geographical preference to present a unified model for new event recommendation. In particular, we utilize a topic model to derive user interests while adopting matrix factorization to infer organizer influences on users from the interactions between users and organizers. Moreover, we model users' geographical preferences via their location histories of attended events. We conduct a performance evaluation using real‐world data collected from DoubanEvent. The experimental results demonstrate the effectiveness of our proposed recommendation model.
Purification of the regulatory component of adenylate cyclase.

The regulatory component (G/F) of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6. 1.1] from rabbit liver plasma membranes has been purified essentially to homogeneity. The purification was accomplished by three chromatographic procedures in sodium cholate-containing solutions, followed by three steps in Lubrol-containing solutions. The specific activity of G/F was enriched 2000-fold from extracts of membranes to 3-4 mumol x min-1 x mg-1 (reconstituted adenylate cyclase activity). Purified G/F reconstitutes guanine nucleotide-, fluoride-, and hormone-stimulated adenylate cyclase activity in the adenylate cyclase-deficient variant of S49 murine lymphoma cells. G/F also recouples hormonal stimulation of the enzyme in the uncoupled variant of S49. Preparations of pure G/F contain three polypeptides with approximate molecular weights of 52,000, 45,000, and 35,000. The active G/F protein behaves as a multisubunit complex of these polypeptides. Treatment of G/F with [32P]NAD+ and cholera toxin covalently labels the molecular weight 52,000 and 45,000 polypeptides with 32P.
Suspected metaldehyde slug bait poisoning in dogs: a retrospective analysis of cases reported to the Veterinary Poisons Information Service

A retrospective analysis of telephone enquiries to the Veterinary Poisons Information Service found 772 cases with follow-up concerning suspected metaldehyde slug bait ingestion in dogs between 1985 and 2010. Half the enquiries occurred in the summer months. The amount and strength of the slug bait ingested was rarely known. In 56, cases the quantity consumed was estimated and was on average 229.6 grams of bait. Clinical signs developed in 77.3 per cent of dogs; common signs were convulsions, hypersalivation, twitching, hyperaesthesia, tremor, vomiting, hyperthermia and ataxia. Only 4.6 per cent of dogs developed hepatic changes, and only one developed renal impairment. The average time to onset of signs was 2.9 hours post-ingestion, with 50.3 per cent of dogs developing effects within one hour. Increased muscle activity (twitching, convulsions) lasted on average 15.2 hours. Recovery time was reported in 61 cases and occurred on average at 39.3 hours. Common treatments were gut decontamination, anticonvulsants, anaesthetics and intravenous fluids. Of the dogs that were treated with sedatives, 45.8 per cent required more than one sedative or anaesthetic agent. Methocarbamol was rarely used, probably due to unavailability. The outcome was reported in 762 dogs; 21.7 per cent remained asymptomatic, 61.7 per cent recovered and 16 per cent of dogs died or were euthanased. Where known (only six cases), the fatal dose of bait ranged from 4.2 to 26.7 g/kg (average 11.8 g/kg).
Curcumin and Cancer Cells: How Many Ways Can Curry Kill Tumor Cells Selectively?

Cancer is a hyperproliferative disorder that is usually treated by chemotherapeutic agents that are toxic not only to tumor cells but also to normal cells, so these agents produce major side effects. In addition, these agents are highly expensive and thus not affordable for most. Moreover, such agents cannot be used for cancer prevention. Traditional medicines are generally free of the deleterious side effects and usually inexpensive. Curcumin, a component of turmeric (Curcuma longa), is one such agent that is safe, affordable, and efficacious. How curcumin kills tumor cells is the focus of this review. We show that curcumin modulates growth of tumor cells through regulation of multiple cell signaling pathways including cell proliferation pathway (cyclin D1, c-myc), cell survival pathway (Bcl-2, Bcl-xL, cFLIP, XIAP, c-IAP1), caspase activation pathway (caspase-8, 3, 9), tumor suppressor pathway (p53, p21) death receptor pathway (DR4, DR5), mitochondrial pathways, and protein kinase pathway (JNK, Akt, and AMPK). How curcumin selectively kills tumor cells, and not normal cells, is also described in detail.
Irrelevant speech does not interfere with serial recall in early blind listeners

Phonological working memory is known be (a) inversely related to the duration of the items to be learned (word-length effect), and (b) impaired by the presence of irrelevant speech-like sounds (irrelevant-speech effect). As it is discussed controversially whether these memory disruptions are subject to attentional control, both effects were studied in sighted participants and in a sample of early blind individuals who are expected to be superior in selectively attending to auditory stimuli. Results show that, while performance depended on word length in both groups, irrelevant speech interfered with recall only in the sighted group, but not in blind participants. This suggests that blind listeners may be able to effectively prevent irrelevant sound from being encoded in the phonological store, presumably due to superior auditory processing. The occurrence of a word-length effect, however, implies that blind and sighted listeners are utilizing the same phonological rehearsal mechanism in order to maintain information in the phonological store.
Membrane vesicle secretion and prophage induction in multidrug‐resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress

Several bacterial species produce membrane vesicles (MVs) in response to antibiotic stress. However, the biogenesis and role of MVs in bacterial antibiotic resistance mechanisms have remained unclear. Here, we studied the effect of the fluoroquinolone ciprofloxacin on MV secretion by Stenotrophomonas maltophilia using a combination of electron microscopy and proteomic approaches. We found that in addition to the classical outer membrane vesicles (OMV), ciprofloxacin-stimulated cultures produced larger vesicles containing both outer and inner membranes termed outer-inner membrane vesicles (OIMV), and that such MVs are enriched with cytosolic proteins. Remarkably, OIMV were found to be decorated with filamentous structures identified as fimbriae. In addition, ciprofloxacin stress leads to the release of bacteriophages and phage tail-like particles. Prophage induction by ciprofloxacin has been linked to pathogenesis and horizontal gene transfer in several bacterial species. Together, our findings show that ciprofloxacin treatment of S. maltophilia leads to the secretion of a heterogeneous pool of MVs and the induction of prophages that are potentially involved in adverse side-effects during antibiotic treatment.
Rapid identification of some Leptospira isolates from cattle by random amplified polymorphic DNA fingerprinting

We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Australian cattle. Each serovar and genotype had a unique RAPD profile. Of 26 field isolates of Leptospira, 23 were identified as hardjo genotype bovis subtype A, 2 were identified as zanoni, and 1 was identified as pomona by RAPD fingerprinting, and their types were confirmed by cross-absorption agglutination and restriction enzyme analysis.
THE IMPACT OF CORRELATED PROJECTIONS ON WEAK LENSING CLUSTER COUNTS

Large-scale structure projections are an obstacle in converting the shear signal of clusters detected in weak-lensing maps into virial masses. However, this step is not necessary for constraining cosmology with the shear-peak abundance, if we are able to predict its amplitude. We generate a large ensemble of N-body simulations spanning four cosmological models, with total volume Vtot ∼ 1(h−1 Gpc)3 per model. Variations to the matter density parameter and amplitude of fluctuations are considered. We measure the abundance of peaks in the mass density projected in ≈100 h−1 Mpc slabs to determine the impact of structures spatially correlated with the simulation clusters, identified by the three-dimensional (3D) friends-of-friends (FoF) algorithm. The halo model shows that the choice of the smoothing filter for the density field is important in reducing the contribution of correlated projections to individual halo masses. Such contributions are less than 2% in the case of the optimal, compensated filter used throughout this analysis. We measure the change in the mass of peaks when projected in slabs of various thicknesses. Peaks in slabs of 26 h−1 Mpc and 102 h−1 Mpc suffer an average mass change of less than 2% compared to their mass in slabs of 51 h−1 Mpc. We then explore the cosmology dependence of the projected-peak mass function, and find that, for a wide range of slab thicknesses (< 500 h−1 Mpc), it scales with cosmology in exactly the same way as the 3D FoF mass function and the Sheth–Tormen (ST) formula. This extends the earlier result of Marian et al. Finally, we show that for all cosmological models considered, the low and intermediate mass bins of the peak abundance can be described using a modified ST functional form to within 10%–20% accuracy.
Securing Systems with Scarce Entropy: LWE-Based Lossless Computational Fuzzy Extractor for the IoT

With advent of the Internet of Things, lightweight devices necessitate secure and cost-efficient key storage. Since traditional secure key storage is expensive, novel solutions have been developed based on the idea of deriving the key from noisy entropy sources. Such sources when combined with fuzzy extractors allow cryptographically strong key derivation. Information-theoretic fuzzy extractors require large amounts of input entropy to account for entropy loss in the key extraction process. It has been shown by Fuller et al. (ASIACRYPT’13) that the entropy loss can be reduced if the requirement is relaxed to computational security based on the hardness of the Learning with Errors problem. Using this computational fuzzy extractor, we show how to construct a device-server authentication system providing outsider chosen perturbation security and pre-application robustness. We present the first implementation of a lossless computational fuzzy extractor where the entropy of the source equals the entropy of the key on a constrained device. The implementation needs only 1.45KB of SRAM and 9.8KB of Flash memory on an 8-bit microcontroller. Furthermore, we also show how a device-server authentication system can be constructed and efficiently implemented in our system. We compare our implementation to existing work in terms of security, while achieving no entropy loss.
Prominent 85‐kDa Oligomannosidic Glycoproteins of Rat Brain Are Signal Regulatory Proteins and Include the SHP Substrate‐1 for Tyrosine Phosphatases

Abstract: The glycoprotein component in rat brain reacting most strongly with Galanthus nivalis agglutinin (GNA) on western blots migrates as an 85‐kDa band. GNA identifies mannose‐rich oligosaccharides because it is highly specific for terminal α‐mannose residues. After purification of this 85‐kDa glycoprotein band by chromatography on GNA‐agarose and preparative gel electrophoresis, binding of other lectins demonstrated the presence of fucose and a trace of galactose, but no sialic acid. Treatment with N‐Glycanase or endoglycosidase H produced a 65‐kDa band, indicating that it consisted of about one‐fourth N‐linked oligomannosidic carbohydrate moieties. High‐performance anion‐exchange chromatography and fluorescence‐assisted carbohydrate electrophoresis indicated that the major carbohydrate moiety is a heptasaccharide with the structure Manα1‐6(Manα1‐3)Manα1‐6(Manα1‐3)Manβ1‐4Glc‐NAcβ1‐4GlcNAc (Man5GlcNAc2). Determination of amino acid sequences of peptides produced by endoproteinase digestion demonstrated that this 85‐kDa mannose‐rich glycoprotein component contained the SHP substrate‐1 for phosphotyrosine phosphatases and at least one other member of the signal‐regulatory protein (SIRP) family. The unusually high content of oligomannosidic carbohydrate moieties on these receptor‐like members of the immunoglobulin superfamily in neural tissue could be of functional significance for intercellular adhesion or signaling.
α-Hydroxybutyric Acid Is a Selective Metabolite Biomarker of Impaired Glucose Tolerance

OBJECTIVE Plasma metabolites that distinguish isolated impaired glucose tolerance (iIGT) from isolated impaired fasting glucose (iIFG) may be useful biomarkers to predict IGT, a high-risk state for the development of type 2 diabetes. RESEARCH DESIGN AND METHODS Targeted metabolomics with 23 metabolites previously associated with dysglycemia was performed with fasting plasma samples from subjects without diabetes at time 0 of an oral glucose tolerance test (OGTT) in two observational cohorts: RISC (Relationship Between Insulin Sensitivity and Cardiovascular Disease) and DMVhi (Diabetes Mellitus and Vascular Health Initiative). Odds ratios (ORs) for a one-SD change in the metabolite level were calculated using multiple logistic regression models controlling for age, sex, and BMI to test for associations with iIGT or iIFG versus normal. Selective biomarkers of iIGT were further validated in the Botnia study. RESULTS α-Hydroxybutyric acid (α-HB) was most strongly associated with iIGT in RISC (OR 2.54 [95% CI 1.86–3.48], P value 5E-9) and DMVhi (2.75 [1.81–4.19], 4E-5) while having no significant association with iIFG. In Botnia, α-HB was selectively associated with iIGT (2.03 [1.65–2.49], 3E-11) and had no significant association with iIFG. Linoleoyl-glycerophosphocholine (L-GPC) and oleic acid were also found to be selective biomarkers of iIGT. In multivariate IGT prediction models, addition of α-HB, L-GPC, and oleic acid to age, sex, BMI, and fasting glucose significantly improved area under the curve in all three cohorts. CONCLUSIONS α-HB, L-GPC, and oleic acid were shown to be selective biomarkers of iIGT, independent of age, sex, BMI, and fasting glucose, in 4,053 subjects without diabetes from three European cohorts. These biomarkers can be used in predictive models to identify subjects with IGT without performing an OGTT.