Miracle-dz/UniFAR-FTUs huggingface dataset:
Relationship Between Community-Level Alcohol Outlet Accessibility and Individual-Level Herpes Simplex Virus Type 2 Infection Among Young Women in South Africa

Background Exposure to alcohol outlets may influence sexual health outcomes at the individual and community levels. Visiting alcohol outlets facilitates alcohol consumption and exposes patrons to a risky environment and network of potential partners, whereas the presence of alcohol outlets in the community may shift social acceptance of riskier behavior. We hypothesize that living in communities with more alcohol outlets is associated with increased sexual risk. Methods We performed a cross-sectional analysis in a sample of 2174 South African schoolgirls (ages 13–21 years) living across 24 villages in the rural Agincourt subdistrict, underpinned by long-term health and sociodemographic surveillance. To examine the association between number of alcohol outlets in village of residence and individual-level prevalent herpes simplex virus type 2 (HSV-2) infection, we used generalized estimating equations with logit links, adjusting for individual- and village-level covariates. Results The median number of alcohol outlets per village was 3 (range, 0–7). Herpes simplex virus type 2 prevalence increased from villages with no outlets (1.4% [95% confidence interval, 0.2–12.1]), to villages with 1 to 4 outlets (4.5% [3.7–5.5]), and to villages with more than 4 outlets (6.3% [5.6, 7.1]). An increase of 1 alcohol outlet per village was associated with an 11% increase in the odds of HSV-2 infection (adjusted odds ratio [95% confidence interval], 1.11 [0.98–1.25]). Conclusions Living in villages with more alcohol outlets was associated with increased prevalence of HSV-2 infection in young women. Structural interventions and sexual health screenings targeting villages with extensive alcohol outlet environments could help prevent the spread of sexually transmitted infections.
Pullulan as a stabilizer agent of polymeric nanocapsules for drug delivery

Polymeric stabilizers have received attention in the preparation of nanostructured systems due to their ability to enhance formulation stability. Considering this, the objective of this work was to prepare poly(e-caprolactone) nanocapsules using the pullulan as a polymeric stabilizer. The nanocapsules were prepared using the interfacial deposition method of preformed polymers and they were characterized in terms of pH, average diameter, polydispersity index, zeta potential, beclomethasone dipropionate content, encapsulation efficiency, photostability and drug release profiles. The formulations showed physicochemical characteristics consistent with nanocarriers for drug delivery such as: average diameter lower than 270 nm, polydispersity indexes lower than 0.2, negative zeta potential (-22.7 to -26.3 mV) and encapsulation efficiencies close to 100%. In addition, the nanocapsules were able to delay the beclomethasone dipropionate photodegradation under UVC radiation and by the dialysis bag diffusion technique, the nanocapsules were able to prolong the drug release. Thus, pullulan could be considered an interesting excipient to formulate polymeric nanocapsules.
Effects of 1 year of growth hormone therapy on serum lipoprotein levels in growth hormone-deficient adults. Influence of gender and Apo(a) and ApoE phenotypes.

We investigated the influence of gender and apoE and apo(a) phenotypes as well as the effect of the metabolic effects of growth hormone (GH) on the effect of GH therapy on serum lipoprotein concentrations in GH-deficient (GHD) adults. Forty-four consecutive patients, 30 men and 14 women aged 46.5 (range, 19 to 76) years with GHD due mainly to pituitary tumors, were treated with recombinant human GH for 12 months. Serum concentrations of lipoproteins, insulin, thyroxine, and insulin-like growth factor-I were determined, body composition was assessed by bioelectrical impedance, and apo(a) and apoE phenotypes were analyzed. Lipoprotein(a) [Lp(a)] concentrations in the GHD subjects were compared with a gender- and apo(a) phenotype-matched control group. After 12 months of GH treatment, the total cholesterol, LDL cholesterol, and apoB concentrations decreased, the HDL cholesterol and apoE concentrations increased, and the apoA-I and triglyceride concentrations were unchanged. Before treatment, the Lp(a) concentration was similar to that in the control group. However, after 12 months of treatment, the Lp(a) concentration had increased by 44% and 101% above baseline and the control group, respectively. Men and women responded differently to GH, with a more marked increase in Lp(a) concentration and fat-free mass and a more pronounced decrease in body-fat mass in men. Apo(a) phenotypes had no major influence on the effect of GH therapy. The only significant difference between apoE phenotypes was a higher baseline Lp(a) concentration among apoE4 heterozygotes. (ABSTRACT TRUNCATED AT 250 WORDS)
Low bBack-off 16-APSK transmission using magnitude modulation and symbol quantization

QAM and APSK are used to meet the ever growing demand for higher data rates in bandwidth limited satellite channels whose main constraint to communication capacity is a non-linear transmitting high power amplifier (HPA). The inherent APSK robustness against nonlinear distortion leads to its introduction in the recent standard for digital video broadcasting (DVB-S2). Power efficiency is still limited by high signal PAPR at the HPA input. Magnitude modulation is a technique for PAPR reduction, proposed with success for QPSK. This paper shows that it is possible to use the magnitude modulation concept for the 16-APSK case, even considering the huge number of symbol combinations. The methodpsilas capability to avoid phase modulation is improved. In order to reduce look-up table computation complexity and storage requirements, the constellation symbols are vector quantized. Constellation and RRC symmetries are also explored. Experimental results show considerable gains of 80% in back-off reduction.
A novel single nucleotide polymorphism altering stability and activity of CYP2a6.

CYP2A6 is known as a major cytochrome P450 (CYP) responsible for the oxidation of nicotine and coumarin in humans. In this study, we explored genetic polymorphisms, which reduce CYP2A6 activity in Japanese. Two novel mutations in exon 9 of the CYP2A6 gene were found. A single nucleotide polymorphism of T1412C and G1454T resulted in Ile471Thr and Arg485Leu substitution, respectively. The frequency of the former variant allele was considerably high (15.7%), while the latter variant appeared to be a rare polymorphism. Heterologous expression of CYP2A6 using a cDNA possessing C instead of T-base at codon 471 in Escherichia coli caused remarkable reduction of the stability of holoenzyme at 37 degrees C. Furthermore, this variant enzyme almost lacked nicotine C-oxidase activity, although coumarin 7-hydroxylase activity was still observed. These data suggest that individuals homozygous for the T1412C variant allele or heterozygous for this and a defect allele such as the CYP2A6*4 may be poor metabolizer of nicotine, but not coumarin.
Tissue Distribution Profiles of the Human TRPM Cation Channel Family

Eight members of the TRP-melastatin (TRPM) subfamily have been identified, whose physiological functions and distribution are poorly characterized. Although tissue expression and distribution patterns have been reported for individual TRPM channels, comparisons between individual studies are not possible because of variations in analysis techniques and tissue selection. We report here a comparative analysis of the expression patterns of all of the human TRPM channels in selected peripheral tissues and the central nervous system (CNS) using two distinct but complimentary approaches: TaqMan and SYBR Green real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). These techniques generated comparative distribution profiles and demonstrated tissue-specific co-expression of TRPM mRNA species, indicating significant potential for the formation of heteromeric channels. TRPM channels 2, 4, 5, 6, and 7 in contrast to 1, 3, and 8 are widely distributed in the CNS and periphery. The tissues demonstrating highest expression for individual family members were brain (TRPM1), brain and bone marrow (TRPM2), brain and pituitary (TRPM3), intestine and prostate (TRPM4), intestine, pancreas, and prostate (TRPM5), intestine and brain (TRPM6), heart, pituitary, bone, and adipose tissue (TRPM7), and prostate and liver (TRPM8). The data reported here will guide the elucidation of TRPM channel physiological functions.
Severe deprivation in twins: a case study.

SUMMARY The author reports an unusual case of deprivation. Monozygotic twin boys were reared from age 18 months to 7 yr in social isolation by a psychopathic stepmother and an inadequate father. On discovery, their mental age level was 3 yr, hilt after treatment, a period in a children's home and approximately 2 yr in a good foster-home, they had made remarkable progress and now appear about average for their age. Forensic aspects of the case are discussed, as arc features of the foster-home placement, and the significance of twins hip in the recovery. Residual effects of deprivation will be studied by an extended follow-up. Because of the criminal behaviour of the stepmother the children were exposed to living conditions which resembled those of an experimental situation. From the human standpoint, of course, it was an extremely cruel and unrepeatable experiment. From the scientific point of view, however, it was also a very valuable our. It will be important, therefore, to follow up the ease over a long period, in the hope of contributing to a solution of some of the problems associated with mental deprivation.
The ALIAS Pilot Trial: A Dose-Escalation and Safety Study of Albumin Therapy for Acute Ischemic Stroke—I: Physiological Responses and Safety Results

Background and Purpose— In preclinical stroke models, high-dose human albumin confers robust neuroprotection. We investigated the safety and tolerability of this therapy in patients with acute ischemic stroke. Methods— The ALIAS (Albumin in Acute Stroke) Pilot Clinical Trial used a multiple-tier, open-label, dose-escalation design. Subjects with acute ischemic stroke (NIH Stroke Scale [NIHSS] of 6 or above) received a 2-hour infusion of 25% human albumin (ALB) beginning within 16 hours of stroke onset. Six successive ALB dose tiers were assessed ranging from 0.34 to 2.05 g/kg. Neurologic and cardiac function was sequentially monitored. At 3 months, the NIHSS, modified Rankin Scale, and Barthel Index were measured. Results— Eighty-two subjects (mean age, 65 years) received ALB at 7.8±3.4 hours after stroke onset (mean±standard deviation). Forty-two patients also received standard-of-care intravenous tissue plasminogen activator (tPA). Vital signs were unaltered by ALB treatment. Dose-related increases in plasma albumin and mild hemodilution were maximal at 4 to 12 hours. Age-related plasma brain natriuretic peptide levels increased at 24 hours after ALB but did not predict cardiac adverse events. The sole ALB-related adverse event was mild or moderate pulmonary edema in 13.4% of subjects, which was readily managed with diuretics. In the tPA-treated subgroup, symptomatic intracranial hemorrhage occurred in only one of 42 subjects. Conclusions— Twenty-five percent human albumin in doses ranging up to 2.05 g/kg was tolerated by patients with acute ischemic stroke without major dose-limiting complications. tPA therapy did not affect the safety profile of ALB. The companion article presents neurologic outcome data and efficacy analysis in these subjects.
Imaging mass spectrometry in transmission geometry.

Imaging mass spectrometry has been used to provide information on the spatial distribution of lipids,[1,2] peptides[3] and proteins,[4] as well as pharmaceuticals[5] and metabolites[6,7] directly from biological tissue sections. Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry has been successful in investigating protein changes corresponding to several diseases, including Alzheimer’s,[8] Parkinson’s,[9,10] and cancer. [11-13] Secondary ion mass spectrometry (SIMS) has been used to map the distribution of cancer agents[14] and lipids[15] with high sensitivity and spatial resolution, although its mass range is limited by extensive molecular fragmentation. [16] Desorption electrospray ionization (DESI), an ambient ionization method, shows promise in the imaging of living tissue without sample preparation,[17,18] but its spatial resolution is currently limited. [19] Common to all surface imaging methods is the ablation from the front side, also referred to as reflection geometry, with the laser beam, cluster beam or liquid stream at an angle to the plane of the surface. Pairing the sensitivity and specificity of mass spectrometry (MS) with imaging techniques has the potential to provide information on the chemical composition of surfaces at the subcellular dimension, but necessitates the development of methods combining high-throughput analysis and high sensitivity with high spatial resolution.
Relationship of volumetric bone density and structural parameters at different skeletal sites to sex steroid levels in women.

CONTEXT Although estrogen clearly plays a central role in regulating bone mass in women, studies in men have suggested that there may be a threshold bioavailable (bio) estradiol (E2) level below which aging men begin to lose bone and that the threshold for estrogen deficiency in cortical bone may be considerably lower than that in trabecular bone. There are no data testing this in women. OBJECTIVE Our objective was to assess volumetric bone mineral density (vBMD) and bone geometry by quantitative computed tomography and relate these to circulating bio E2 and bio testosterone levels. DESIGN We studied a cross-sectional, age-stratified population sample of 235 women (age, 21-97 yr). RESULTS vBMD/structural parameters were not related to sex steroid levels in young premenopausal women (age, 20-39 yr) with a median bio E2 level of 17 pg/ml (63 pmol/liter). By contrast, bio E2 and bio testosterone levels were both significantly associated with trabecular and cortical vBMD and cortical area at multiple sites in late postmenopausal women (age > or = 60 yr) who had a median bio E2 level of 3 pg/ml (11 pmol/liter). Late premenopausal and early postmenopausal women (age, 40-59 yr) with an intermediate median bio E2 level of 11 pg/ml (42 pmol/liter) showed age-adjusted correlations of bio E2 levels with trabecular but not with cortical vBMD. CONCLUSIONS In women, bio E2 levels are associated with vBMD and some structural bone parameters at low but not high bio E2 levels. Similar to findings in men, the threshold for estrogen deficiency in cortical bone in women appears to be lower than that in trabecular bone.
The Role of Debt and Equity Finance over the Business Cycle

This paper documents that debt and equity issuance are procyclical for most size-sorted firm categories of listed U.S. firms. The procyclicality of equity issuance decreases monotonically with firm size. At the aggregate level, however, the results are not conclusive. The reason is that issuance is countercyclical for very large firms which, although few in number, have a large effect on the aggregate because of their enormous size. We show that the shadow price of external funds is procyclical if firms use the standard one-period contract. This model property generates procyclical equity and - as in the data - the procyclicality decreases with firm size. Another factor that causes equity to be procyclical in the model is a countercyclical cost of equity issuance. The calibrated model (i) generates a countercyclical default rate, (ii) generates a stronger cyclical response for small firms, and (iii) magnifies shocks, whereas the model without equity as an external financing source does the exact opposite.
Antisense Phosphorodiamidate Morpholino Oligomer Length and Target Position Effects on Gene-Specific Inhibition in Escherichia coli

ABSTRACT Phosphorodiamidate morpholino oligomers (PMOs) are synthetic DNA analogs that inhibit gene expression in a sequence-dependent manner. PMOs of various lengths (7 to 20 bases) were tested for inhibition of luciferase expression in Escherichia coli. Shorter PMOs generally inhibited luciferase greater than longer PMOs. Conversely, in bacterial cell-free protein synthesis reactions, longer PMOs inhibited equally or more than shorter PMOs. Overlapping, isometric (10-base) PMOs complementary to the region around the start codon of luciferase inhibited to different extents in bacterial cell-free protein expression reactions. Including the anti-start codon in PMOs was not required for maximal inhibition. PMOs targeted to 5′ nontranslated or 3′ coding regions within luciferase mRNA did not inhibit, except for one PMO targeted to the ribosome-binding site. Inhibition of luciferase expression correlated negatively with the predicted secondary structure of mRNA regions targeted by PMO but did not correlate with C+G content of targeted regions. The effects of PMO length and position were corroborated by using PMOs (6 to 20 bases) targeted to acpP, a gene required for viability. Because inhibition by PMOs of ∼11 bases was unexpected based on previous results in eukaryotes, we tested an 11-base PMO in HeLa cells and reticulocyte cell-free protein synthesis reactions. The 11-base PMO significantly inhibited luciferase expression in HeLa cells, although less than did a 20-base PMO. In reticulocyte cell-free reactions, there was a trend toward more inhibition with longer PMOs. These studies indicate that strategies for designing PMOs are substantially different for prokaryotic than eukaryotic targets.
Pathology as it relates to ear surgery II. Labyrinthectomy

Five human temporal bones and three surgical VIIIth nerve biopsies from patients who had previously undergone labyrinthectomy, five months to ten years before examination, were studied by light and electron microscopy. Post-labyrinthectomy dizziness could be explained on the bases of inadequate surgical removal of the vestibular sense-organs, neuroma formation in the vestibule, and high regenerative potential of the vestibular nerve. Post-labyrinthectomy pressure sensation and tinnitus are most probably due to cochlear endolymphatic hydrops and need cochlear neurectomy. The severe atrophy in the sensorineural structures of the cochlea was not associated with retrograde degeneration of the cochlear nerve central axons. This may be of significance in the artificial electrical stimulation of the cochlear nerve in deaf patients.
Fibromyalgia in Behçet’s disease: a narrative review

Introduction: Fibromyalgia is characterised by chronic widespread pain and tenderness. It has often been reported to occur concomitantly with chronic rheumatological conditions. Behçet’s disease is a chronic relapsing, multisystem, autoinflammatory disease. There is only limited understanding of a potential relationship between fibromyalgia and Behçet’s disease. Aim: Given the potential detrimental influence of pain on the outcome of chronic disease, the aim of this narrative review is to gain an understanding of the incidence and presentation of fibromyalgia in Behçet’s disease. Methods: Electronic databases Scopus, Medline, PubMed and UpToDate were searched. Results: A total of 269 studies were identified, and limitations and exclusion/inclusion criteria were applied to ensure accurate and comparable selection of studies; four studies were selected. All cases were assessed for the presence of fibromyalgia according to the 1990 or 2010 diagnostic criteria of the American College of Rheumatology, with Behçet’s disease diagnosed according to the International Study Group (ISG) for Behçet’s disease criteria. A higher prevalence of fibromyalgia (5.7–37.1%) was reported in Behçet’s disease compared to that of the general population (2.9–4.7%). Discussion: While an increased prevalence of fibromyalgia was found in patients with Behçet’s disease, this needs to be considered within the context of limited available evidence. The potential impact of these conditions on the disease activity of each other is not clear and may require a prospective study. Conclusion: Fibromyalgia appears to be more prevalent in those with Behçet’s disease than would be expected in the overall population. Significance: This review provides some evidence that fibromyalgia is more prevalent in those with Behçet’s disease. To ensure appropriate patient treatment choices, it is important that both conditions are diagnosed where they co-exist.
Knowledge management in local government: The case of Stellenbosch Municipality

Knowledge management (KM) was a well-founded management approach that held significant benefits for public sector organisations. KM as a management philosophy had an impact on various components of an organisation and it could therefore significantly advance organisational efficiency. The successful implementation of KM initiatives had to be ensured because, different organisational processes and departments had to collaborate and functional silos had to be eliminated. KM required long-term commitment and dedication from all organisational members. Furthermore, there were certain knowledge management enablers in an organisation that needed to be developed and that were necessary for the achievement of organisational effectiveness. These enablers were the organisational culture, human resources, information technology, organisational structure and, the organisation’s strategy and leadership. This article assessed each of these enablers and how they impacted on an organisation’s KM efforts. Stellenbosch Municipality was used as a case in point. The article further examined how local governments were able to effectively implement KM practices as strategic tools used to achieve service delivery and operational goals. The article concluded with a number of recommended strategies, (1) to develop the KM enablers that were present in organisations and (2) to aid the implementation of successful organisational KM initiatives.
Linkage map of Pseudomonas aeruginosa PAT

The locations of new markers relative to markers previously mapped on the chromosome of Pseudomonas aeruginosa strain PAT were defined by generalized transduction with phage F116L and F1083. Although the marker orders of the various marker groups were deduced mainly from the results of two-factor crosses, the locations of a number of markers were confirmed by three-factor crosses. A linkage map of the chromosome of P. aeruginosa PAT was constructed which shows the relative locations of 50 genes. From the available data, the linkage maps of P. aeruginosa strains PAO and PAT appear to be similar.
DS+: Reliable Distributed Snapshot Algorithm for Wireless Sensor Networks

Acquiring the snapshot of a distributed system helps gathering system related global state. In wireless sensor networks (WSNs), global state shows if a node is terminated or deadlock occurs along with many other situations which prevents a WSN from fully functioning. In this paper, we present a fully distributed snapshot acquisition algorithm adapted to tree topology wireless sensor networks (WSNs). Since snapshot acquisition is through control messages sent over highly lossy wireless channels and congested nodes, we enhanced the snapshot algorithm with a sink based reliability suit to achieve robustness. We analyzed the performance of the algorithm in terms of snapshot success ratio and response time in simulation and experimental small test bed environment. The results reveal that the proposed tailor made reliability model increases snapshot acquisition performance by a factor of seven and response time by a factor of two in a 30-node network. We have also shown that the proposed algorithm outperforms its counterparts in the specified network setting.