Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of the alpha6 integrin as a candidate receptor for papillomaviruses

Papillomaviruses (PVs) bind in a specific and saturable fashion to a range of epithelial and other cell lines. Treatment of cells with trypsin markedly reduces their ability to bind virus particles, suggesting that binding is mediated via a cell membrane protein. We have investigated the interaction of human PV type 6b L1 virus-like particles (VLPs) with two epithelial cell lines, CV-1 and HaCaT, which bind VLPs, and a B-cell line (DG75) previously shown not to bind VLPs. Immunoprecipitation of a mixture of PV VLPs with [35S]methionine-labeled cell extracts and with biotin-labeled cell surface proteins identified four proteins from CV-1 and HaCaT cells of 220, 120, 87, and 35 kDa that reacted with VLPs and were not present in DG75 cells. The alpha6beta4 integrin complex has subunits corresponding to the VLP precipitated proteins, and the tissue distribution of this complex suggested that it was a candidate human PV receptor. Monoclonal antibodies (MAbs) to the alpha6 or beta4 integrin subunits precipitated VLPs from a mixture of CV-1 cell proteins and VLPs, whereas MAbs to other integrin subunits did not. An alpha6 integrin-specific MAb (GoH3) inhibited VLP binding to CV-1 and HaCaT cells, whereas an anti-beta4 integrin MAb and a range of integrin-specific and other MAbs did not. Furthermore, human laminin, the natural ligand for the alpha6beta4 integrin, was able to block VLP binding. By use of sections of monkey esophagus, the distribution of alpha6 integrin expression in the basal epithelium was shown to coincide with the distribution of bound VLPs. Taken together, these data suggest that VLPs bind specifically to the alpha6 integrin subunit and that integrin complexes containing alpha6 integrin complexed with either beta1 or beta4 integrins may act as a receptor for PV binding and entry into epithelial cells.
Truth and Truthmakers

The A. wants to reconcile maximalisme about truthmakers (every truth has a truthmaker) and the relation of necessitation (every contingent truth can be used in a truthmaker for a necessary truth). He investigates the metaphysical sense of the implication and whether one can admit de re contingency. He examines the pure possibilities and the case of alien properties according with David Lewis and studies whether it is contingent that there is something rather than nothing. He analyses the truthmakers of necessary truths and adopts a possibilist thesis for matters of demonstrative science. He concludes with the transcategorial (and metaphysical) necessity which is implicit in the generalization of truthmakers.
In vitro antiviral activity of neem (Azardirachta indica L.) bark extract against herpes simplex virus type‐1 infection

Herpes simplex virus type 1 (HSV‐1) causes significant health problems from periodical skin and corneal lesions to encephalitis. We report here that an aqueous extract preparation from the barks of neem plant Azardirachta indica acts as a potent entry inhibitor against HSV‐1 infection into natural target cells. The neem bark extract (NBE) significantly blocked HSV‐1 entry into cells at concentrations ranging from 50 to 100 μg/ml. The blocking activity of NBE was observed when the extract was pre‐incubated with the virus but not with the target cells, suggesting a direct antiHSV‐1 property of the neem bark. Further, virions treated with NBE failed to bind the cells which implicate a role of NBE as an attachment step blocker. Cells treated with NBE also inhibited HSV‐1 glycoprotein‐mediated cell–cell fusion and polykaryocytes formation suggesting an additional role of NBE at the viral fusion step. These findings open a potential new avenue for the development of NBE as a novel antiherpetic microbicide. Copyright © 2009 John Wiley & Sons, Ltd.
Working Environment and work retention

The project shows that the working environment is rarely the sole cause of early retirement, but working environment factors may explain a large fraction of the transition to disability pension in particular. Several working environment predictors of early retirement are well-documented in the Nordic countries, but workplace policies and activities to retain older workers rarely aim to improve the working environment. There may be great potentials in developing workplace interventions which combine prevention of working environment risk factors (e.g. risk factors for accidents, strenuous work/high physical work demands, insufficient recovery between work shifts, quantitative work demands, conflicts at work and bullying/harassment, and age discrimination) with increment of job satisfaction through increased control/influence, possibilities for development and recognition from management.
Estrogen formation in human prostatic tissue from patients with and without benign prostatic hyperplasia

Prostatic tissue removed at the time of cystoprostatectomy was separated into periurethral and peripheral zones. Homogenized tissue was incubated with [1,2,6,73H] androstenedione in the presence or absence of an aromatase inhibitor, 4‐hydroxyandrostenedione (4‐OHAD) and a 5α‐reductase inhibitor 4‐MA (N,N‐diethyl‐4‐methyl‐3‐oxo‐4‐aza‐5α‐androstane 17β‐carboxamide). Estrogen formation was determined by reverse isotope dilution of [3H] estrone and [3H] estradiol and crystallization to constant specific activity. Control incubations were carried out in parallel utilizing heated prostatic tissue. Total estrogens produced in the periurethral zone in patients with benign prostatic hyperplasia (BPH) was 223 fmol/ mg protein/hr (SE ± 57) compared to 102 fmol (SE ± 17) in patients without BPH. Estrogen formation in the peripheral zone was 175 fmol (SE ± 69) and 105 fmol (SE ± 26) in patients with and without BPH, respectively. The prostatic aromatase exhibits Michaelis‐Menton kinetics with an apparent Km of 90 nM. 4‐OHAD inhibited aromatization in the prostatic tissue by 57‐93%. Aromatization was also strongly inhibited by 4‐MA, indicating that 4‐MA is a potent aromatase as well as a 5α‐reductase inhibitor in this tissue. These results suggest that aromatization of androgens to estrogens in the human prostate proceeds at a substantial rate and that local estrogen formation could preexist and be a factor in the etiology of BPH and prostatic cancer.
Instantons, Topological Strings and Enumerative Geometry

We review and elaborate on certain aspects of the connections between instanton counting in maximally supersymmetric gauge theories and the computation of enumerative invariants of smooth varieties. We study in detail three instances of gauge theories in six, four, and two dimensions which naturally arise in the context of topological string theory on certain noncompact threefolds. We describe how the instanton counting in these gauge theories is related to the computation of the entropy of supersymmetric black holes and how these results are related to wall-crossing properties of enumerative invariants such as Donaldson-Thomas and Gromov-Witten invariants. Some features of moduli spaces of torsion-free sheaves and the computation of their Euler characteristics are also elucidated.
Expression of proteinase-activated receptors in mouse microglial cells

Microglia are the resident immune cells of the CNS: they are activated rapidly by CNS damage and perform the function of tissue macrophages. The first steps during microglial activation are currently under intense study, and it is widely believed that substances released from damaged brain tissue can trigger this process. We recently reported that the blood coagulation factor thrombin, which enters the CNS during breakdown of the blood–brain barrier, activates microglial cells. The cellular effects of thrombin and trypsin-like proteases are mediated by proteinase-activated receptors (PARs). Based on our prior data we hypothesized that microglial cells express these receptors. Using RT-PCR and flow cytometry, we report that primary mouse microglial cells, as well as the murine microglial cell lines BV-2 and N9, indeed express PARs, albeit at different levels. Demonstrating multiple PARs on microglia may enhance the attractiveness of PARs as therapeutic targets in neuroinflammatory disorders.
Expression of genes of type I and type II collagen in the formation and development of the blastema of regenerating newt limb

We cloned cDNAs of α1(I) and α1(II) collagen, and studied their expression profiles in regenerating limbs of newts, Cynops pyrrhogaster. The expression of the α1(I) gene was markedly up‐regulated at the early bud stage of the blastema. In situ hybridization experiments revealed that the α1(I) gene was expressed in not only mesenchymal cells of the blastema, but also the basal cells of the wound epidermis at the wound healing stage when the epidermal basement membrane was absent. This unique expression continued until 21 days (late bud stage), while the basement membrane began to form at 14 days. These results indicate biochemical differences between the wound and normal epidermis, and suggest the direct involvement of the former in the synthesis of blastemal matrices of type I collagen. Actually, immunohistochemistry revealed that type I collagen began to be deposited beneath the wound epidermis at 8 days, and accumulated there and around blastemal mesenchymal cells at 14 to 21 days. Undifferentiated mesenchymal cells associated with the amputated muscle fibers actively expressed the α1(I) gene. Mesenchymal cells in the central region of blastemas deposited type I collagen fibers around them. Concomitantly with the appearance of prechondrocytes, the α1(II) collagen gene became activated. The present study clearly shows that the expression of the genes of both type I and type II collagen in blastemal cells is temporally and regionally well‐regulated in a cooperative manner. Dev Dyn 1999;216:59–71. © 1999 Wiley‐Liss, Inc.
APLIKASI TECHNOLOGY ACCEPTANCE MODEL PADA SISTEM INFORMASI MANAJEMEN RUMAH SAKIT

The purpose of this study was to apply the Technology Acceptance Model (TAM) in the Management Information Systemof Prof. Dr. R. Soeharso Orthopedics Hospital in Surakarta. This study was an empirical research with quantitative method. The data used comprised of primary and secondary ones. The study population was all employees of Prof. Dr. R. Soeharsowho use the management information system, i.e. 382 people. By using simple random method inthe sampling techniques,193 people were selected as the study sample. The results show that: computer self-efficacy,positively and significantly affects the perception of the benefits and the perceived ease of technology usein the information system of the hospital management; and so subjective norms, itpositively and significantlyaffects the perception of the benefits and the perceived ease of technology use in the hospital management information system; system accessibilitydoes not significantly affect the perception of the benefits, but it gives positive and significant influenceon the perceived ease of technology use in the information system of the hospital management; there isa significant and positive influencefrom the perceived ease of technology use against the perceived benefits of the information system, however, that beneficial perception does not affect the attitude of technology use in the information system of hospital management;the perception of the ease of technology use does not significantly affect the perceived attitude in using the technology, nonetheless, that attitude about the technology use has positive and significant influence against behavioral intention to use the technology in the hospital management information system; and that intention in the technology use, subsequently affects significantly and positively the behavior of the use of the same technology in the hospital management information system ofProf. Dr. R. SoeharsoOrthopedics Hospital of Surakarta. Key words : Technology Accepance Model, Computer Self Efficacy, Subjective Norms, System Accessibility, Perception of Technology Benefits, Attitude of Technology Use, Intention of Technology Use, Behavior of Technology Use,Hospital Management Information System
Hybrid Proxy Re-encryption Between IBE And CBE

In proxy re-encryption, a proxy can transform a ciphertext computed under Alice’s public key into one that can be opened under Bob’s decryption key. In 2007, Matsuo proposed the concept of four types of proxy re-encryption schemes: CBE(Certificate Based Public Key Encryption) to IBE(Identity Based Encryption)(type 1), IBE to IBE(type 2), IBE to CBE (type 3), CBE to CBE (type 4). We observe that the proxy re-encryption from CBE to IBE scheme in Matsuo’s scheme inherits the key escrow problem from IBE. Is this necessary for proxy re-encryption from CBE to IBE? We give a negative answer. If we emphasis on the PKG’s involving in the re-encryption key generation, some interesting results can be obtained. We propose the concept of hybrid proxy re-encryption without key escrow, give the new security model for this primitive, construct such a scheme and prove its security. Furthermore, we construct the first proxy re-encryption scheme from from IBE to CBE, giving the security model for this new primitive and prove its security. At last, we compare our schemes with other related schemes, the results show that our schemes can have high level security with good efficiency.
Setting the pace of the Neurospora circadian clock by multiple independent FRQ phosphorylation events

Protein phosphorylation plays essential roles in eukaryotic circadian clocks. Like PERIOD in animals, the Neurospora core circadian protein FRQ is progressively phosphorylated and becomes extensively phosphorylated before its degradation. In this study, by using purified FRQ protein from Neurospora, we identified 43 in vivo FRQ phosphorylation sites by mass spectrometry analysis. In addition, we show that CK-1a and CKII are responsible for most FRQ phosphorylation events and identify an additional 33 phosphorylation sites by in vitro kinase assays. Whole-cell metabolic isotope labeling and quantitative MS analyses suggest that circadian oscillation of the FRQ phosphorylation profile is primarily due to progressive phosphorylation at the majority of these newly discovered phosphorylation sites. Furthermore, systematic mutations of the identified FRQ phosphorylation sites led to either long or short period phenotypes. These changes in circadian period are attributed to increases or decreases in FRQ stability, respectively. Together, this comprehensive study of FRQ phosphorylation reveals that regulation of FRQ stability by multiple independent phosphorylation events is a major factor that determines the period length of the clock. A model is proposed to explain how FRQ stability is regulated by multiple phosphorylation events.
Laterally Coupled DFB Lasers for One-Step Growth Photonic Integrated Circuits in InP

Design and characterization of a transmitter building block for one-step growth photonic integration, featuring a 1310 - nm laterally coupled distributed-feedback laser with a front-side passive optical waveguide and a back-side optical power monitor, are presented. Formed on a semi-insulating Fe : InP substrate and processed by means of a stepper optical lithography, the device perfectly suits the multiguide vertical integration-a newly developed one-step growth photonic integrated technique in InP.
Deglutitive laryngeal closure in stroke patients

Background: Dysphagia has been reported in up to 70% of patients with stroke, predisposing them to aspiration and pneumonia. Despite this, the mechanism for aspiration remains unclear. Aims: To determine the relationship between bolus flow and laryngeal closure during swallowing in patients with stroke and to examine the sensorimotor mechanisms leading to aspiration. Methods: Measures of swallowing and bolus flow were taken from digital videofluoroscopic images in 90 patients with stroke and 50 healthy adults, after repeated volitional swallows of controlled volumes of thin liquid. Aspiration was assessed using a validated Penetration–Aspiration Scale. Oral sensation was also measured by electrical stimulation at the faucial pillars. Results: After stroke, laryngeal ascent was delayed (mean (standard deviation (SD)) 0.31 (0.06) s, p<0.001), resulting in prolongation of pharyngeal transit time (1.17 (0.07) s, p<0.001) without a concomitant increase in laryngeal closure duration (0.84 (0.04) s, p = 0.9). The delay in laryngeal elevation correlated with both the severity of aspiration (r = 0.5, p<0.001) and oral sensation (r = 0.5, p<0.001). Conclusions: After stroke, duration of laryngeal delay and degree of sensory deficit are associated with the severity of aspiration. These findings indicate a role for sensorimotor interactions in control of swallowing and have implications for the assessment and management of dysphagia after stroke.
Designed to Fail: Effects of the Default Option and Information Complexity on Student Loan Repayment

We ask why so few student loan borrowers enroll in Income Driven Repayment when the majority would benefit from doing so. To do so we run an incentivized laboratory experiment using a facsimile of the government’s Student Loan Exit Counseling website. We test the role information complexity, uncertainty about earnings, and the default option play. We show that despite an ex ante optimal choice, the majority choose, or are defaulted into, a plan that offers no protection against default. We find the default option is a driver of this phenomenon, suggesting the government has an easy policy lever to lower default rates – change the default plan.
PRENATAL MATERNAL BIOCHEMISTRY PREDICTS NEONATAL BIOCHEMISTRY

Depressed (n = 45) and nondepressed (n = 47) mothers were recruited prenatally at an ultrasound clinic. Their urine samples were assayed for cortisol, catecholamines (norepinephrine, epinephrine, dopamine) and serotonin. Their urines were assayed again at the neonatal period, and their newborns' urines were also assayed at that time. The depressed versus the nondepressed mothers showed significantly higher cortisol and norepinephrine and significantly lower dopamine levels across the pre- and postnatal assessments. At the postnatal assessment all levels had decreased except the serotonin levels for both groups. Regression analyses on the mother's postnatal biochemistry with the prenatal biochemistry entered as predictor variables showed highly significant, specific relationships between each of the catecholamines, cortisol, and serotonin. The newborn's biochemistry (except for epinephrine) was higher than the maternal biochemistry. Regression analyses on the neonatal biochemistry with the mother's prenatal biochemistry entered as predictor- variables also suggested highly significant, specific relationships. The continuity between the mother's and the newborn's neurotransmitter/-neurohormone profiles and data showing that elevated norepinephrine and cortisol predict to low birthweight and prematurity, respectively, highlight the importance of assessing these levels during pregnancy.
Interrelationships between Public Open Space, Common Pool Resources, Publicness Levels and Commons Dilemmas: A Different Perspective in Urban Planning

Public open space (POS) is central to the environment, and oftentimes spatial and architectural designs are emphasised in urban planning as part of creating quality POS. However, such initial design and planning of POS may not adequately encapsulate the sustainability dimensions of the complex social-ecological behavioural patterns of POS consumption and management, hence resulting in space mismanagement, underinvestment, and quality degradation. This phenomenon is particularly true and relevant in the context of government/state-owned POS. Therefore, an objective of this perspective paper, coupled with the concepts of the publicness levels, is to provide a different understanding of exclusivity and subtractibility natures of POS, primarily using the theory of common pool resources (CPRs), which subsequently helps explain and rationalise the perennial, adversarial POS management, quality and sustainability status quo. This paper reveals that, instead of being considered as pure public goods, scarce POS owns two inherent attributes of CPR, namely non-excludable and subtractive (rivalrous) that are ultimately susceptible to social/commons dilemmas, covering the Tragedy of the commons (overexploitation), management shirking, free-riding, underuse, disuse, and moral hazard, which lead to degraded, unsustainable POS. The commons or CPR theory can indeed offer a new paradigm shift, making urban planners and landscape managers to embrace that the unexclusive natures of CPR-based POS are truly finite and depletable and thus vulnerable to POS dilemmas. Hence, to achieve quality, sustainable POS commons, effective governance in terms of consumption and consistent management is vital. For future research, urban design as a necessary societal role is suggested, which has established the need for effective allocation of POS management via an adaptive institutional property rights design.
PERANAN PERTUMBUHAN EKONOMI DAN INFLASI TERHADAP KINERJA INVESTASI ASING LANGSUNG (FDI) DI NEGARA ASEAN

Investasi asing langsung (FDI) merupakan faktor penting dalam menggerakkan pertumbuhan ekonomi suatu negara terutama bagi negara-negara berkembang. Pertumbuhan ekonomi merupakan salah satu faktor penarik investasi langsung. Kinerja inflasi di negara-negara berkembang dinilai cukup tinggi dibanding negara-negara maju sehingga berpengaruh dalam pengambilan keputusan oleh investor terutama FDI. Dalam hal ini inflasi merupakan refleksi dari biaya investasi, dimana bila tingkat inflasi tinggi maka biaya investasi juga semakin tinggi sehingga nilai FDI akan menurun. Tujuan dilakukannya penelitian ini adalah untuk melihat pengaruh pertumbuhan ekonomi dan inflasi terhadap investasi asing langsung di empat negara ASEAN yaitu Indonesia, Malaysia, Filipina, dan Thailand. Data yang digunakan adalah data time serius dengan jangka waktu 2000-2017. Uji yang digunakan adalah uji asumsi klasik dan regresi berganda. Hasil penelitian dapat disimpulkan pertumbuhan ekonomi dan inflasi berpengaruh siginifikan terhadap investasi asing langsung di negara ASEAN baik secara parsial maupun secara bersama-sama.
CSF levels of β‐amyloid 1‐42, tau and phosphorylated tau protein in CADASIL

Background and purpose: Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) can be considered a useful model of pure subcortical vascular dementia (SVD) because it occurs in young adults, unlikely to have concomitant age‐ and Alzheimer’s disease (AD)‐related pathology. In patients with CADASIL we evaluated the cerebrospinal fluid (CSF) levels of β‐amyloid 1‐42 (Aβ42), total tau protein (t‐tau) and phosphorylated tau protein (p‐tau), which are well‐accepted biomarkers of AD.
An Experimental Investigation of Epistemic Modal Adverbs and Adjectives

This paper analyses epistemic modal adverbs and adjectives, both theoretically and experimentally, while addressing the prevalent conceptions of modality and context update. While modality is standardly viewed and represented uniformly, we show that epistemic modal adverbs and adjectives differ in various linguistic environments, and present experimental evidence that supports the claim that epistemic modal adverbs and adjectives differ in terms of cognitive processing. While context update is standardly viewed as a two-stage process composed of assertion and acceptance/rejection, we present experimental evidence that supports the claim that there is also a stage of evaluation, in which the hearer considers the content of the assertion and the implications of adding this content to the common ground.