Miracle-dz/UniFAR-FTUs huggingface dataset:
Mesenchymal Stem Cells Regulate Angiogenesis According to Their Mechanical Environment

In fracture and bone defect healing, MSCs largely drive tissue regeneration. MSCs have been shown to promote angiogenesis both in vivo and in vitro. Angiogenesis is a prerequisite to large tissue reconstitution. The present study investigated how mechanical loading of MSCs influences their proangiogenic capacity. The results show a significant enhancement of angiogenesis by conditioned media from mechanically stimulated compared with unstimulated MSCs in two‐dimensional tube formation and three‐dimensional spheroid sprouting assays. In particular, proliferation but not migration or adhesion of endothelial cells was elevated. Promotion of angiogenesis was dependent upon fibroblast growth factor receptor 1 (FGFR1) signaling. Moreover, stimulation of tube formation was inhibited by vascular endothelial growth factor receptor (VEGFR) tyrosine kinase blocking. Screening for the expression levels of different soluble regulators of angiogenesis revealed an enrichment of matrix metalloprotease 2, transforming growth factor β1, and basic fibroblast growth factor but not of vascular endothelial growth factor in response to mechanical stimulation. In conclusion, mechanical loading of MSCs seems to result in a paracrine stimulation of angiogenesis, most likely by the regulation of a network of several angiogenic molecules. The underlying mechanism appears to be dependent on the FGFR and VEGFR signaling cascades and might be mediated by an additional cross‐talk with other pathways.
Psychosocial correlates of mild visual loss.

&NA; Studies of the psychosocial aspects of visual impairment have emphasized the effects of blindness, giving relatively little attention to the effects of mild or partial visual impairment. Consequently, we know little about when in the course of visual loss significant psychosocial dysfunction develops. To address this question, we assessed psychosocial functioning at three times over eight months in 31 adults with proliferative diabetic retinopathy and mild to moderate visual impairment in at least one eye. Examination of the correlations between visual and psychosocial measures revealed strong and significant correlations between visual acuity and adjustment (range of r = ‐0.45 to ‐0.68), between visual acuity and psychological symptoms (range of r = ‐0.39 to ‐0.50), and between visual acuity and emotion‐focused coping (range of r = ‐0.38 to ‐0.53). The strength of these correlations and their occurrence in three independent measures of psychosocial functioning suggest a clinically meaningful relationship between visual and psychosocial functioning in the range of mild to moderate visual impairment. Psychosocial dysfunction related to visual impairment develops long before blindness. Further prospective research on the psychosocial aspects of partial visual impairment will clarify this relationship and may help justify early intervention with rehabilitation in the visually impaired who do not qualify for services for the blind.
The role of sex-specific normative beliefs in undergraduate alcohol use.

OBJECTIVES To create explanatory models of 3 undergraduate drinking practices based on sex-specific norms. METHODS An electronic, student survey at one Mid-western university produced a representative sample of college students. RESULTS Multivariate analyses indicated that close-friend norms were the best predictors of drinking frequency, quantity, and drunkenness. With one exception, typical student (or distal) norms had no significant relationship to drinking. Opposite-sex norms had associations with drinking above and beyond that explained by same-sex norms. CONCLUSIONS The findings challenge the current application of the popular social norms approach that relies on distal drinking norms to provide normative feedback.
Radar cross section (RCS) modeling and simulation, part 1: a tutorial review of definitions, strategies, and canonical examples

Modeling and simulation strategies for radar cross section (RCS) prediction are reviewed, and a novel FDTD-based virtual RCS prediction tool is introduced in this two-part paper. Part 1 is reserved for a tutorial review. Concepts and definitions related to RCS modeling are outlined. Analytical approaches, i.e., high-frequency asymptotics (HFA), as well as powerful time- and frequency-domain numerical methods, are given. Canonical examples using the finite-difference time-domain (FDTD) Method, the method of moments (MoM), and physical optics (PO) are presented.
Cancer stem cells: An insight

Cancer stem cells (CSCs) have the ability to self-renew and are present in most tissues including breast, brain, lung, head and neck, prostates, testis, ovary, esophagus, colon and liver. Their origin is yet to be discovered though a series of hypotheses have been proposed in this regard. CSCs play a role in not only the creation of cancer but also its evolution, metastasis and recurrence. CSCs have an important role in cancer therapy and the resistance toward chemotherapeutic agents. This article reviews the characteristics of CSCs in terms of origin, methods of isolation and cancer therapy.
Expression of cytokine mRNA in gastric mucosa with Helicobacter pylori infection.

BACKGROUND We have studied the cytokine production patterns in gastric mucosal biopsy specimens with and without the Helicobacter pylori infection, using a reverse transcription-polymerase chain reaction (RT-PCR) method capable of detecting low levels of specific mRNA. METHODS Total RNA was prepared from biopsy specimens with the acid guanidinium thiocyanate-phenol-chloroform method. cDNA was synthesized by M-MLV RTase and amplified using the oligonucleotide primers specific for interleukin-1 beta (IL-1beta), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, tumor necrosis factor-alpha (TNF-alpha), interferon-alpha (IFN-alpha), IFN-beta, and IFN-gamma by PCR methods. RESULTS Although IL-1 beta and IFN-gamma mRNA were detected in most specimens, IL-2, IL-3, IL-4, IL-5, IL-9, TNF-alpha, and IFN-beta mRNA were not detected at all. The expressions of IL-7 and IL-8 mRNA were significantly higher in H. pylori-positive gastritis than in H. pylori-negative normal controls. There was a significant correlation between the expression of IL-8 mRNA and the severity of gastritis both in the antrum and in the corpus. On the other hand, there was a significant correlation between the expression of IL-7 mRNA and the severity of gastritis only in the corpus. CONCLUSIONS These findings suggest that some cytokines, especially IL-7 and IL-8, play some roles in H. pylori-associated gastritis.
Effect of Methionine Source and Dietary Crude Protein Level on Growth Performance, Carcass Traits and Nutrient Retention in Chinese Color-feathered Chicks

A total of 1,200 LinNan Chinese color-feathered chicks were used to study the effects of methionine source [DL-2-hydroxy-4-methylthio-butanoic acid (HMTBa) or DL-methionine (DLM)] and dietary crude protein (CP) level on growth performance, carcass traits, and whole-body nitrogen and fat retention. The trial was designed as a 2x2 factorial arrangement, including two CP levels (adequate and low) and two methionine sources (HMTBa and DL-methionine). Diets were formulated for three phases, starter (0-21 d), grower (21-42 d), and finisher (42-63 d). Chicks fed HMTBa had higher daily gain and improved feed efficiency than DLM during the grower phase (p 0.05). Chicks fed low-CP diets grew slower, used feed less efficiently during the grower, finisher phase and overall. On d 42, regardless of dietary CP levels, birds fed HMTBa had higher carcass weights, breast and thigh weights than DLM-fed birds (p<0.04). Birds fed low-CP diet had lighter carcass weights and less breast muscle, thigh muscle, and dressing percentage at the end of starter, grower and finisher phases (p<0.05). Whole body composition analyses found that birds fed HMTBa tended to contain more protein and less fat compared to those chicks fed DLM at the end of the starter phase (p<0.10). Low-CP diets increased CP concentration in the whole body at the end of the finisher phase (p = 0.05). HMTBa supplementation increased whole-body N retention rate during the finisher phase and overall (p<0.01), and low-CP diets reduced N intake and whole-body fat retention during the finisher phase and overall (p<0.05). In summary, HMTBa was better than DLM on an equimolar basis for growth performance, carcass traits, and N retention in Chinese color-feathered chicks. Low-CP diets lowered growth performance as well as carcass traits in color-feathered birds, probably due to imbalanced AA profiles.
Secondary Ice Production: Current State of the Science and Recommendations for the Future

AbstractMeasured ice crystal concentrations in natural clouds at modest supercooling (temperature ~>−10°C) are often orders of magnitude greater than the number concentration of primary ice nucleating particles. Therefore, it has long been proposed that a secondary ice production process must exist that is able to rapidly enhance the number concentration of the ice population following initial primary ice nucleation events. Secondary ice production is important for the prediction of ice crystal concentration and the subsequent evolution of some types of clouds, but the physical basis of the process is not understood and the production rates are not well constrained. In November 2015 an international workshop was held to discuss the current state of the science and future work to constrain and improve our understanding of secondary ice production processes. Examples and recommendations for in situ observations, remote sensing, laboratory investigations, and modeling approaches are presented.
Variation in VIP latrine sludge contents

This study investigated variations in the characteristics of the sludge content from different ventilated improved pit (VIP) latrines and variation in these characteristics at specific depths within each pit. Faecal sludge from 16 VIP latrines within the eThekwini Municipality was collected and laboratory characterisation including moisture content, total and volatile solids, chemical oxygen demand, and aerobic biodegradability was performed. Sludge samples were collected from 4 specific depths within each pit investigated. The laboratory characterisation performed showed that none of the VIP latrines investigated had the same sludge characteristics, and that within a pit sludge characteristics varied with increasing depth in the pit. This supports the motivating hypothesis that, depending on household habits and local environmental conditions, there should be considerable variation in the organic contents, moisture content, non-biodegradable content and microbial population between different pits. This variation with increasing depth within a pit is expected, since fresh material is constantly being added to the pit overlaying older material which might have undergone a certain degree of stabilisation.
On the Brain of Dasypus Sexcinctus.

IN most systematic treatises on Comparative Anatomy incidental notices of the brains of the Edentata occur, and in several memoirs especially devoted to the anatomy of various members of this family more'detailed descriptions are given. Tiedemann 1 (PL. v. Fig. 8) figures and describes the brain of Myrmecophaga didactylus. Rapp' figures and describes the brain of Bradypus cuculliger. Hyrtl3 describes the brain of Chlamydophorus truncatus, and compares it with that of Dasypus gymnurus. Tiedemann (P1. v. Fig. 10), Rapp and Alessandrino' describe and figure the brain of Dasypus novemcinctus, and Tiedemann (P1. iv. Figs. 9 & 10) and Flower' have given figures and descriptions of the brain of Bradypus (Cbolcepus) didactylus. I have seen no special reference to the brain of Dasypus sexcinctus; and as I recently acquired a perfectly fresh adult female specimen of this animal I purpose giving an account of its cerebral characters. Dimensions. Inches. Extreme length of encephalon . 2-0 Length of olfactory lobe . 0 5 Length of inner face of cerebral hemisphere . 1 1 Length of cerebellum . 016 Greatest breadth of cerebrum . 1X5 Greatest breadth of cerebellum . 1 0
Assessment of the Effects of Poultry Litter on Surface Runoff Water Quality from Agricultural Lands

The use of pelletized poultry litter (PPL) as a substitute for inorganic fertilizers is increasingly being en-couraged in states like Delaware which have a considerable surplus of poultry litter. However, we know very little about the impacts of PPL on runoff water quality and whether it is an environmentally-sound and sus-tainable alternative to inorganic fertilizer. To address these questions we compared the exports of nutrients (NH4-N, NO3-N and PO4-P) and trace elements (As, Cu, and Zn) in surface runoff from agricultural plots receiving PPL, raw poultry litter (RPL), urea and no-fertilizer (control) treatments. The study was conducted on agricultural land located in Middletown, Delaware with corn as the cover crop. The experimental plots were 5 m wide and 12 m long with reduced tillage and no-tillage management practices. Sampling was con-ducted for six natural rainfall events from April through August 2008. Nutrient (NH4-N, NO3-N and PO4-P) exports from plots receiving PPL were less than those with urea or raw litter applications. While exports of trace elements from the PPL treatment exceeded those from urea, they were much lower than the corre-sponding exports from the RPL treatments. Mass exports of nutrients and trace elements were correlated with event size (rainfall amount) but were not correlated with timing of event (days since litter application). Results from this study suggest that the use of PPL in combination with no-tillage may provide an environ-mentally safe alternative to synthetic fertilizers.
Evidence that interaction of hepatocytes with the collecting (hepatic) veins triggers position-specific transcription of the glutamine synthetase and ornithine aminotransferase genes in the mouse liver

We previously demonstrated that glutamine synthetase (GS) and ornithine aminotransferase (OAT) mRNAs are expressed in the mouse liver acinus preferentially in pericentral hepatocytes, that is, those immediately surrounding terminal central veins (A.L. Bennett, K.E. Paulson, R.E. Miller, and J.E. Darnell, Jr., J. Cell Biol. 105:1073-1085, 1987, and F.C. Kuo, W.L. Hwu, D. Valle, and J.E. Darnell, Jr., Proc. Natl. Acad. Sci. USA, in press). We now show that hepatocytes surrounding large collecting hepatic veins but not portal veins also express these two mRNAs. The pericentral hepatocytes are the most distal hepatocytes with respect to acinar blood flow, whereas this is not necessarily the case for hepatocytes next to the large collecting hepatic veins. This result implies that it is contact with some hepatic venous element which signals positional expression. In an effort to induce conditions that change relationships between hepatocytes and blood vessels, regenerating liver was studied. After surgical removal of two-thirds or more of the liver, there was no noticeable change in GS or OAT expression in the remaining liver tissue during regeneration. However, treatment with carbon tetrachloride (CCl4), which specifically kills pericentral hepatocytes, completely removed GS- and OAT-containing cells and promptly halted hepatic transcription of GS. Repair of CCl4 damage is associated with invasion of inflammatory and scavenging cells, which remove dead hepatocytes to allow regrowth. Only when hepatocytes resumed contact with pericentral veins were the pretreatment levels of OAT and GS mRNA and high levels of GS transcription restored.
Pay for Performance in Primary Care in England and California: Comparison of Unintended Consequences

PURPOSE We undertook an in-depth exploration of the unintended consequences of pay-for-performance programs In England and California. METHODS We interviewed primary care physicians in California (20) and England (20) and compared unintended consequences in each setting. Interview recordings were transcribed verbatim and subjected to thematic analysis. RESULTS Unintended consequences reported by physicians varied according to the incentive program. English physicians were much more likely to report that the program changed the nature of the office visit. This change was linked to a larger number of performance measures and heavy reliance on electronic medical records, with computer prompts to facilitate the delivery of performance measures. Californian physicians were more likely to express resentment about pay for performance and appeared less motivated to act on financial incentives, even in the program with the highest rewards. The inability of Californian physicians to exclude individual patients from performance calculations caused frustration, and some physicians reported such undesirable behaviors as forced disenrollment of noncompliant patients. English physicians are assessed using data extracted from their own medical records, whereas in California assessment mostly relies on data collected by multiple third parties that may have different quality targets. Assessing performance based on these data contributes to feelings of resentment, lack of understanding, and lack of ownership reported by Californian physicians. CONCLUSIONS Our study findings suggest that unintended consequences of incentive programs relate to the way in which these programs are designed and implemented. Although unintended, these consequences are not necessarily unpredictable. When designing incentive schemes, more attention needs to be paid to factors likely to produce unintended consequences.
Dissecting the brain's fear system reveals the hypothalamus is critical for responding in subordinate conspecific intruders

Effective defense against natural threats in the environment is essential for the survival of individual animals. Thus, instinctive behavioral responses accompanied by fear have evolved to protect individuals from predators and from opponents of the same species (dominant conspecifics). While it has been suggested that all perceived environmental threats trigger the same set of innately determined defensive responses, we tested the alternate hypothesis that different stimuli may evoke differentiable behaviors supported by distinct neural circuitry. The results of behavioral, neuronal immediate early gene activation, lesion, and neuroanatomical experiments indicate that the hypothalamus is necessary for full expression of defensive behavioral responses in a subordinate conspecific, that lesions of the dorsal premammillary nucleus drastically reduce behavioral measures of fear in these animals, and that essentially separate hypothalamic circuitry supports defensive responses to a predator or a dominant conspecific. It is now clear that differentiable neural circuitry underlies defensive responses to fear conditioning associated with painful stimuli, predators, and dominant conspecifics and that the hypothalamus is an essential component of the circuitry for the latter two stimuli.
THE EFFECT OF Δ1‐TETRAHYDROCANNABINOL ON THE RELEASE OF [3H]‐(‐)‐NORADRENALINE FROM THE ISOLATED VAS DEFERENS OF THE RAT

1 The amount of tritium released upon transmural stimulation of the isolated vas of the rat incubated with [3H]‐Δ1‐tetrahydrocannabinol (THC) (2.8 μM) was significantly greater than the non‐stimulated overflow. 2 There was no difference between non‐stimulated overflow and the effect of transmural stimulation of vasa equilibrated with [14C]‐sorbitol (6.5 mg/ml). 3 The difference between non‐stimulated and stimulated efflux from vasa equilibrated with [3H]‐Δ1‐THC was abolished in rats pretreated for 24 h with 6‐hydroxydopamine (250 mg/kg). 4 The amount of tritium released upon transmural stimulation of the vas incubated with [3H]‐noradrenaline (6 μm) was significantly greater than the non‐stimulated overflow. 5 Δ1‐THC (560 Nm and 14 μm) caused a significant dose‐dependent reduction of both non‐stimulated and stimulated tritium efflux, especially the latter.
Placental Membrane and Placental Disc Microscopic Chorionic Cysts Share Similar Clinicopathologic Associations

Placental membrane microscopic chorionic cysts (MCC) are associated with clinical conditions and placental features of in utero hypoxia. Those occurring in the cell islands, chorionic plate, placental septa, and maternal floor of the chorionic disc have been thought to have degenerative/liquefactive or secretory etiology and no clinical significance. To study the clinical and placental associations of MCC, 24 clinical (maternal and fetal) and 46 gross and microscopic placental features were statistically compared among 266 consecutive cases with MCC (study group, SG) and 656 consecutive placentas without MCC (control group, CG). The SG was further subdivided into (A) 139 placentas with chorionic disc MCC (at least 3 cysts per 1 placental parenchyma section), (B) 93 cases with membrane MCC (at least 3 chorionic lakes per membrane roll), and (C) 34 cases with both membrane and chorionic disc MCC in the same placenta. The SG contained more cases than the CG of diabetes mellitus, fewer cases with preterm premature ruptures of membranes, more multiple pregnancies, less chorioamnionitis, more cases of massive perivillous fibrin deposition, and more cases involving an excessive amount of chorionic disc extravillous trophoblasts. Within the SG, statistically significant differences among SGA, SGB, and SGC groups were found in terms of maternal diabetes mellitus, total perinatal mortality, neonatal mortality, cesarean deliveries, chorioamnionitis, histological meconium staining, chorangiosis, and excessive amount of chorionic disc extravillous trophoblast, respectively. Both membrane and chorionic disc MCC, frequently associated with an excessive amount of extravillous trophoblast, should be regarded as members of the family of extravillous trophoblastic lesions associated with chronic placental hypoxia.
The CLEC12A receptor marks human basophils: Potential implications for minimal residual disease detection in acute myeloid leukemia

The transmembrane receptor C‐type lectin domain family 12, member A (CLEC12A) is known to be highly expressed on monocytes and neutrophils and is a reliable leukemia associated marker in acute myeloid leukemia. Consequently, detailed knowledge of the various normal cell types expressing this receptor is essential. We have observed CLEC12A to be expressed on CD45lowSSClowCD14‐CD123+ basophils in peripheral blood (PB) and in this study, we aimed at verifying this observation and further delineate the CD45lowSSClowCD14‐CD123 + CLEC12A+ subpopulation.