Miracle-dz/UniFAR-FTUs huggingface dataset:
Diminished Human Immunodeficiency Virus Type 1 Reverse Transcription and Nuclear Transport in Primary Macrophages Arrested in Early G1 Phase of the Cell Cycle

ABSTRACT Previously, we and others have demonstrated that the process of reverse transcription of human immunodeficiency virus type 1 (HIV-1) is disturbed in nondividing macrophages and quiescent T lymphocytes. Here we analyzed which phase of the cell cycle in macrophages is crucial for early steps in the HIV-1 replication cycle. HIV-1 Ba-L-inoculated macrophages arrested early in the G1 phase byn-butyrate contained incomplete products of reverse transcription. In gamma-irradiated macrophages, reverse transcription was successfully completed but proviral integration could not be detected. In these cells, nuclear import was disturbed as reflected by the absence of two-long-terminal-repeat circles. In macrophages arrested late in G1 phase by aphidicolin or 5,6-dichloro-1-β-d-ribofuranosyl-benzimidazole (DRB), reverse transcription was unaffected. Proviral integration occurred efficiently in DRB-treated macrophages, whereas integrated proviral DNA could not be detected after aphidicolin treatment. Arrest at G2 phase of the cell cycle by nocodazole did not affect reverse transcription or proviral integration. Treatment of macrophages with hydroxyurea (HU), which reduces the intracellular deoxynucleoside triphosphate (dNTP) pool by blocking the de novo synthesis of dNTP, resulted in a dose-dependent inhibition of HIV-1 reverse transcription. This could partially be restored by the addition of nucleoside precursors. Addition of nucleoside precursors enhanced both reverse transcription and cell proliferation. However, the disturbed reverse transcription observed in the nonproliferating andn-butyrate-treated macrophages could not be restored by addition of nucleoside precursors. Similar to observations in quiescent T lymphocytes, incomplete proviral DNA species were arrested in the cytoplasm of the macrophages. Our results indicate that also in primary macrophages the intracellular nucleotide pools and other cellular factors that coincide with late G1 phase of the cell cycle may contribute to efficient reverse transcription and nuclear localization.
Chronic Diarrhoea Caused by Cryptococcus neoformans in a Non-human Immunodeficiency Virus-infected Patient

A 43-y-old woman with underlying cervix carcinoma stage IIIB, status postirradiation, and diabetes mellitus with tripathy suffered from chronic diarrhoea for more than 6 months. Stool examination showed few white blood cells and red blood cells. Rectal swab cultures disclosed Cryptococcus neoformans in 2 samples obtained 2 weeks apart. The diarrhoea responded to treatment with oral fluconazole for 4 weeks. Repeated rectal swabs revealed no yeast growth. During follow-up for 4 y, she had no problems with diarrhoea. Chronic diarrhoea caused by C. neoformans is rare and clinical suspicion is needed.
Mapping resource use over a Russian landscape: an integrated look at harvesting of a non-timber forest product in central Kamchatka

Small-scale resource use became an important adaptive mechanism in remote logging communities in Russia at the onset of the post-Soviet period in 1991. We focused on harvesting of a non-timber forest product, lingonberry (Vaccinium vitis-idaea), in the forests of the Kamchatka Peninsula (Russian Far East). We employed an integrated geographical approach to make quantifiable connections between harvesting and the landscape, and to interpret these relationships in their broader contexts. Landsat TM images were used for a new classification; the resulting land-cover map was the basis for linking non-spatial data on harvesters’ gathering behaviors to spatial data within delineated lingonberry gathering sites. Several significant relationships emerged: (1) mature forests negatively affected harvesters’ initial choice to gather in a site, while young forests had a positive effect; (2) land-cover type was critical in determining how and why gathering occurred: post-disturbance young and maturing forests were significantly associated with higher gathering intensity and with the choice to market harvests; and (3) distance from gathering sites to villages and main roads also mattered: longer distances were significantly correlated to more time spent gathering and to increased marketing of harvests. We further considered our findings in light of the larger ecological and social dynamics at play in central Kamchatka. This unique study is an important starting point for conservation- and sustainable development-based work, and for additional research into the drivers of human–landscape interactions in the Russian Far East.
346 Bayram and Erol Childhood Traumatic Experiences , Anxiety , and Depression Levels in Fibromyalgia and Rheumatoid Arthritis pain

Method: A total of 30 patients with fibromyalgia, 30 patients with rheumatoid arthritis, and 30 healthy controls, matched with patients with respect to gender, age, and education, were included in the study (90 participants in total). All participants were given a form for sociodemographic characteristics, the Childhood Trauma Questionnaire (CTQ), and Hospital Anxiety and Depression Scale (HAD). Patients were also asked to complete a numeric pain scale (NPS).
Chapter 9 - Societal inequality, health, and well-being

“A house may be large or small; as long as the neighboring (sic) houses are likewise small, it satisfies all social requirement for a residence. But let there arise next to the little house a palace, and the little house shrinks to a hut. The little house now makes it clear that its inmate has no social position at all to maintain, or but a very insignificant one; and however high it may shoot up in the course of civilization, if the neighboring (sic) palace rises in equal of even in greater measure, the occupant of the relatively little house will always find himself more uncomfortable, more dissatisfied, more cramped within his four walls. ” (Marx, 1847)
The History of Trade Unionism

CONTENTS. Chapter: I. The Origins of Trade Unionism. II. The Struggle for Existence [1799-1825]. III. The Revolutionary Period [1829-1842]. IV. The New Spirit and the New Model [1843-1860]. V. The Junta and their Allies [1860-1875]. VI. Sectional Developments [1863-1885]. VII. The Old Unionism and the New [1875-1889]. VIII. The Trade Union World [1892-94]. Map of Trade Unionism. Appendix: — On the assumed connection between the Trade Unions and the Gilds in Dublin — Sliding Scales — The Summons to the First Trade Union Congress — Distribution of Trade Unionists in the United Kingdom — The Progress in Membership of particular Trade Unions — List of Publications on Trade Unions and Combinations of Workmen.
The study of the relationship between organizational structure and psychological empowerment among the staffs in Ministry of Economic Affairs and Finance

The present paper has been conducted with the aim of studying the relationship between organizational structure and psychological empowerment of staff of the Ministry of Economic Affairs and Finance. This research is applied and is survey study from conduct point of view. Research population includes all the staff, experts and managers of Financial Supervision Department and Treasury of the whole country of the Ministry of Economic Affairs and Finance which is a total number of 537 persons. For this purpose we have used closed questionnaires in the form of five options (standard questionnaire of Robins’ organizational structure which has three dimensions of formality, complexity and focus and standard questionnaire of Whetten and Cameron’s psychological empowerment which has 5 dimensions of trust, meaningfulness, effectiveness, self-efficiency, and self-determination). The size of the sample is 224 individuals of staff, experts and managers which have been selected with the use of stratified sampling. For analyzing the obtained data descriptive statistics (frequency table) and inferential statistics (Pearson’s correlation coefficient test) have been used which the results indicate that there is significant and negative relationship between organizational structure and three dimensions of “formality”, “complexity”, “focus” with psychological empowerment variable. Dimension of focus has the highest significance (first rank) among other dimensions of organizational structure variable and also self-determination has the highest importance (first rank) among the dimensions of psychological empowerment. Normal 0 false false false RU X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Обычная таблица"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;}
Diode Laser Transscleral Cyclophotocoagulation for the Treatment of Refractory Glaucoma After Penetrating Keratoplasty

Purpose: To evaluate the effect and safety of diode laser transscleral cyclophotocoagulation (TSCPC) in eyes with refractory glaucoma after penetrating keratoplasty (PK). Methods: Diode laser TSCPC was performed on 32 eyes of 32 patients with secondary glaucoma after penetrating keratoplasty. The mean follow-up period was 11.4 ± 3.8 months (range, 6–20 months). The intraocular pressure (IOP), number of glaucoma medications, graft status, side effects, and complications of the procedure were all recorded during the follow-up period. The treatment was considered successful if after one cyclodiode treatment the IOP could be reduced to or below 22 mmHg with or without medication. The success rate of the diode laser treatment was analyzed by the Kaplan-Meier survival analysis method during the 6th and 12th months of the follow-up period and the end of the study. The decrease in drug requirement after laser treatment was analyzed by the Wilcoxon nonparametric test. Statistical significance was set at p < 0.05. Results: During the follow-up period, diode laser TSCPC reduced the IOP significantly. Our cumulative success rate was 56% at the end of the study. Cyclodiode treatment resulted in an IOP less than 22 mmHg in 97% of the eyes on the 6th month and 72% of the eyes on the 12th month with or without medication. The total retreatment rate of the study was 44%. More than one treatment was necessary, 8 times in the 1st month, 9 times in the 3rd month, 5 times in the 6th month and 3 times in the 12th month. The average number of antiglaucomatous drugs used was 2.8 before surgery and 1.2 after the 12th month follow-up period (p < 0.05). No eyes with graft failure after treatment were present. Visual acuity improved (> 2 Snellen lines of acuity) in two eyes and remained the same in the others. No serious side effects such as phthisis bulbi or hypotonia were observed. Conclusions: Diode laser TSCPC appears to be a safe and effective procedure for the treatment of uncontrolled glaucoma secondary to penetrating keratoplasty.
Volatility forecasting: evidence from a fractional integrated asymmetric power ARCH skewed-t model

Predicting the one-step-ahead volatility is of great importance in measuring and managing investment risk more accurately. Taking into consideration the main characteristics of the conditional volatility of asset returns, an asymmetric Autoregressive Conditional Heteroscedasticity (ARCH) model is estimated. The model is extended to also capture (i) the skewness and excess kurtosis that the asset returns exhibit, and (ii) the fractional integration of the conditional variance. The model, which takes into consideration both the fractional integration of the conditional variance as well as the skewed and leptokurtic conditional distribution of innovations, produces the most accurate one-day-ahead volatility forecasts. The study recommends to portfolio managers and traders that extended ARCH models generate more accurate volatility forecasts of stock returns.
Novel Isopentenyladenosine Analogues: Synthesis, Characterization, and Evaluation of Antiproliferative Activity on Bladder Carcinoma Cells

Isopentenyladenosine (iPA), a member of the cytokinin family of plant hormones, exerts a marked antiproliferative activity on some leukemic and epithelial cancer cell lines. To characterize the molecular moieties required for the in vitro antitumor activity of the molecule and to obtain cytostatic iPA derivatives potentially useful as chemotherapeutic agents, N9-acyclic analogues have been synthesized using regioselective Mitsunobu reaction and characterized by elemental analyses, 1H and 13C NMR. These compounds were analyzed for their activity on human bladder cancer cell lines. In this study, we report that iPA inhibited the proliferation but not the migration of human bladder cancer cells, while the newly synthesized analogues revealed no significant cytostatic activity apart from the compound with a saturated double bond of the isopentenyl chain. These results indicate that the integrity of the ribose ring is required for the cytostatic activity of iPA.
Use of Benchmarks in Predicting Earnings Management?

This study conducts multiple approaches to identify whether earnings benchmarks are an indicator for earnings management within the Australian market. We investigate firms reporting small positive earnings and small positive earnings changes, referred to as benchmark beaters. Accrual quality models, earnings distributions and earnings persistence measures are applied to identify whether benchmark beating firms are manipulating earnings. Our findings suggest that the small positive earnings benchmark attracts earnings managers. However, we do not identify any evidence to indicate that the positive earnings change benchmark is a signal for earnings management.
The Effect of Disclosures by Management, Analysts, and Business Press on Cost of Capital, Return Volatility, and Analyst Forecasts: A Study Using Content Analysis

ABSTRACT: We document systematic evidence of risk effects of disclosures culled from a virtually exhaustive set of sources from the print medium. We content analyze more than 100,000 disclosure reports by management, analysts, and news reporters (i.e., business press) in constructing firm‐specific disclosure measures that are quantitative and amenable to replication. We expect credibility and timeliness differences in the disclosures by source, which would translate into differential cost of capital effects. We find that when content analysis indicates favorable disclosures, the firm's risk, as proxied by the cost of capital, stock return volatility, and analyst forecast dispersion, declines significantly. In contrast, unfavorable disclosures are accompanied by significant increases in risk measures. Analysis of disclosures by source—corporations, analysts, and the business press—reveals that negative disclosures from business press sources result in increased cost of capital and return volatility, and fa.
Molecularly genetic analysis of von Hippel‐Lindau associated central nervous system hemangioblastoma

Von Hippel‐Lindau (VHL) disease is an autosomal dominant inherited cancer predisposition syndrome, characterized by development of a variety of neoplasms in multiple organs. Central nervous system hemangioblastoma (CHB) is the most common manifestation of VHL disease. The germline mutations in the VHL tumor suppressor gene are responsible for the inherited cancer predisposition syndrome. To expand the VHL mutation data and to investigate the tumorigenesis of VHL‐associated CNS hemangioblastoma, 24 CHB tissue samples and blood samples of 14 patients from 10 Chinese VHL families were collected and subjected to molecular genetic analysis. Six distinctive germline mutations were identified, and the 601 G to C (Val130Phe) mutation is reported for the first time. Somatic mutations analysis of the VHL gene in VHL‐associated CHB showed that loss of heterozygosity (LOH) occurred in more than half of the cases. In addition, expression of the ZAC1 tumor suppressor gene protein was studied using immunohistochemistry staining in CHB tissues with a specific polyclonal antibody. The ZAC1 protein was lost in all CHB. Our data exhibited high frequency of LOH of ZAC1 gene in VHL‐associated CHB, indicating that ZAC1 may have a role in tumorigenesis of VHL‐associated CHB.
MULTIPLE PARAMETER RADIATION INJURY ASSESSMENT USING A NONHUMAN PRIMATE RADIATION MODEL—BIODOSIMETRY APPLICATIONS

There are urgent needs to establish capability to rapidly assess radiation injury in mass casualty and population monitoring scenarios. This study's objective was to evaluate several currently available biomarkers that can provide early diagnostic triage information after radiation exposure. Hematology and blood chemistry measurements were performed on samples derived from a nonhuman primate (Macaca mulatta; n = 8) total-body irradiation (TBI) model (6.5-Gy 60Co &ggr; rays at 0.6 Gy min−1). The results from this study demonstrate: a) time course for changes in C-reactive protein (CRP) (−2 d to 15 d after TBI); b) time-dependent (−2 d, 1–4 d after TBI) changes in blood cell counts [i.e., lymphocytes decrease to 5–8% of pre-study levels at 1 to 4 d after TBI; ratio of neutrophil to lymphocytes increases by 44 ± 18 (p = 0.016), 12 ± 4 (p = 0.001), 8 ± 2 (p = 0.0020), and 5.0 ± 2 (p = 0.002) fold at 1, 2, 3, and 4 days after TBI, respectively]; and c) 4.5 ± 0.8 (p = 0.002)-fold increases in serum amylase activity 1 d after TBI. Plasma CRP levels at 1 d after exposure were 22 ± 13 (p = 0.0005) (females) and 44 ± 11 (p = 0.0004) (males)-fold elevated above baseline levels. One hundred percent successful separation of samples from exposed macaques (24 h after TBI) vs. samples from the same macaque taken before irradiation using a discriminant analysis based on four biomarkers (i.e., lymphocytes, neutrophils, ratio of neutrophils to lymphocytes, and serum amylase activity) was demonstrated. These results demonstrate the practical use of multiple parameter biomarkers to enhance the discrimination of exposed vs. non-exposed individuals and justify a follow-on rhesus macaque dose-response study.
N‐methyl‐D‐aspartate receptor channel blockers prevent pentylenetetrazole‐induced convulsions and morphological changes in rat brain neurons

Alterations in inhibitory and excitatory neurotransmission play a central role in the etiology of epilepsy, with overstimulation of glutamate receptors influencing epileptic activity and corresponding neuronal damage. N‐methyl‐D‐aspartate (NMDA) receptors, which belong to a class of ionotropic glutamate receptors, play a primary role in this process. This study compared the anticonvulsant properties of two NMDA receptor channel blockers, memantine and 1‐phenylcyclohexylamine (IEM‐1921), in a pentylenetetrazole (PTZ) model of seizures in rats and investigated their potencies in preventing PTZ‐induced morphological changes in the brain. The anticonvulsant properties of IEM‐1921 (5 mg/kg) were more pronounced than those of memantine at the same dose. IEM‐1921 and memantine decreased the duration of convulsions by 82% and 37%, respectively. Both compounds were relatively effective at preventing the tonic component of seizures but not myoclonic seizures. Memantine significantly reduced the lethality caused by PTZ‐induced seizures from 42% to 11%, and all animals pretreated with IEM‐1921 survived. Morphological examination of the rat brain 24 hr after administration of PTZ revealed alterations in the morphology of 20–25% of neurons in the neocortex and the hippocampus, potentially induced by excessive glutamate. The expression of the excitatory amino acid transporter 1 protein was increased in the hippocampus of the PTZ‐treated rats. However, dark neurons did not express caspase‐3 and were immunopositive for the neuronal nuclear antigen protein, indicating that these neurons were alive. Both NMDA antagonists prevented neuronal abnormalities in the brain. These results suggest that NMDA receptor channel blockers might be considered possible neuroprotective agents for prolonged seizures or status epilepticus leading to neuronal damage. © 2014 Wiley Periodicals, Inc.
Ectopic expression of MyoD1 in mice causes prenatal lethalities

A variety of differentiated cell types can be converted to skeletal muscle following transfection with the myogenic regulatory gene MyoD1. To determine whether MyoD1 is a dominant muscle regulator in vivo, mouse fertilized eggs were microinjected with a β‐actin/MyoD1 gene. Ectopic expression of MyoD1 during mouse embryogenesis led to embryonic lethalities, the cause of which is not known. Transgenic embryos died before midgestation. The majority of tested embryos between 7.5 and 9.5 days, although retarded compared to control littermates, differentiated normally into tissues representative of all three germ layers. In most transgenic embryos there was no indication of myogenic conversion. The expression of the introduced gene was detected in all ectodermal and mesodermal tissues but was absent in all endodermal cells. Forced expression of MyoD1 was associated with the activation of myogenin and MLC2 (but not myf5 or MRF4) genes in non‐muscle cell types, demonstrating the dominant regulatory function of MyoD1 during development. These results demonstrate that ectopic MyoD1 expression and activation of myogenin and MLC2 have no significant effects in the determination of cell lineages or the developmental fate of differentiated mesodermal and ectodermal cell lineages. © 1993 wiley‐Liss, Inc.
The Securitisation of Sri Lankan Tourism in the Absence of Peace

The military conflict in Sri Lanka may be over officially, but conflict continues as a ‘war without sound’ (community informant, Mullaitivu 2013), or as war by other means (Dahlman 2011). In the absence of peace and reconciliation, but the presence of economic growth, development by stealth proceeds. Much has been written about the militarisation of civilian life in Sri Lanka (Kadirgamar 2013; David 2013), but this paper focuses specifically on how militarisation has proceeded with little public protest or pushback. The political work accomplished by ‘securitisation’ is used to gain consent and create new space and capacity for state security measures and militarisation. This paper recasts the connections between security, peace, and development in post-war Sri Lanka, drawing on fieldwork in one area that connects all of these projects: tourism. An analysis of ‘war tourism’ in Sri Lanka shows how it reproduces threats to Sri Lanka’s security at the same time that it celebrates military victory and might. Tourism encapsulates economic, security, and development agendas in very specific ways. Tourist sites mobilise fear of potential terrorism and return to the rule of the Liberation Tigers of Tamil Eelam (LTTE), if vigilance and militarisation are not maintained. In such a context of risk, development is best done by the military. Within this logic of securitisation, militarisation becomes a common sense approach. How is this common sense produced? The securitisation of development is vivid in the post-war context of Sri Lanka, inextricably tied to neoliberal imperatives to convey a democratic, stable country that is open to and good for business.