Miracle-dz/UniFAR-FTUs huggingface dataset:
Myocardial high energy phosphate stores in cardiac hypertrophy and heart failure.

The aim of this study was to determine whether the impairment of contractility in the myocardium obtained from hypertrophied and failing hearts is due to a decreased store of energy and whether an imbalance exists between energy production and energy utilization in these hearts in vivo. Right ventricular hypertrophy and right ventricular failure were produced in cats by constriction of the main pulmonary artery. Concentrations of creatine phosphate (CP) and adenosine triphosphate (ATP) were determined in samples removed from the right ventricles of these animals during life and in papillary muscles isolated from these same hearts. The papillary muscles from the cats with hypertrophy and failure exhibited depressed intrinsic contractility. The stores of ATP were normal in both the ventricular muscles and the papillary muscles in animals with hypertrophy and failure. Although the stores of CP were significantly depressed in the right ventricles of these animals, the stores had increased toward normal in papillary muscles isolated from these same hearts in vitro. The finding of normal energy stores in the papillary muscles from animals with hypertrophy and failure indicates that their intrinsically depressed contractility cannot be due to reductions of their energy stores in vivo such as were found in the samples from the ventricular wall. The differences between high energy phosphate stores in failing heart in vivo and these stores as they are replenished in vitro also indicate that the in vivo depressions are secondary to an imbalance between energy production and energy utilization in the overloaded, hypertrophied and failing heart. ADDITIONAL
Analyses of Nursing Home Residents With Cancer at Admission

The objective of the study was to profile nursing home residents with cancer at admission to the nursing facility. We used all admission assessments in the Minimum Data Set recorded throughout the United States during 2002 to identify 61,890 residents with cancer, or 11.3% of all admissions. Nursing home residents with cancer were significantly older and more likely to be male than other residents at admission. Large proportions of nursing home residents with cancer were activities of daily living dependent and about 55% used a wheelchair as their primary mode of locomotion. More than 37% of residents with cancer experienced at least moderate daily pain at admission and almost 26% had a diagnosis of depression. At admission, more than half of residents with cancer had an unstable health condition, 21% were judged to be in their final 6 months of life, and 19% received hospice care. More than 40% of residents with cancer had no advance directives recorded at admission. Compared to other residents at admission, larger proportions of residents with cancer require heavy care. Staff at nursing facilities need to address depression, pain management, and the implementation of advance directives to improve the quality of life for residents with cancer.
Trigeminal neuralgia without neurovascular compression presents earlier than trigeminal neuralgia with neurovascular compression.

OBJECT Trigeminal neuralgia (TN) occurs and recurs in the absence of neurovascular compression (NVC). To characterize what may be distinct patient populations, the authors examined age at onset in patients with TN with and without NVC. METHODS A retrospective review of patients undergoing posterior fossa surgery for Type I TN at Oregon Health & Science University from 2009 to 2013 was undertaken. Charts were reviewed, and imaging and operative data were collected for patients with and without NVC. Mean, median, and the empirical cumulative distribution of onset age were determined. Statistical analysis was performed using Student t-test, Wilcoxon and Kolmogorov-Smirnoff tests, and Kaplan-Meier analysis. Multivariate analysis was performed using a Cox proportional hazards model. RESULTS The charts of 219 patients with TN were reviewed. There were 156 patients who underwent posterior fossa exploration and microvascular decompression or internal neurolysis: 129 patients with NVC and 27 without NVC. Mean age at symptoms onset for patients with and without NVC was 51.1 and 42.6 years, respectively. This difference (8.4 years) was significant (t-test: p = 0.007), with sufficient power to detect an effect size of 8.2 years. Median age between groups with and without NVC was 53.25 and 41.2 years, respectively (p = 0.003). Histogram analysis revealed a bimodal age at onset in patients without NVC, and cumulative distribution of age at onset revealed an earlier presentation of symptoms (p = 0.003) in patients without NVC. Chi-square analysis revealed a trend toward female predominance in patients without NVC, which was not significant (p = 0.08). Multivariate analysis revealed that age at onset was related to NVC but not sex, symptom side or distribution, or patient response to medical treatment. CONCLUSIONS NVC is neither sufficient nor necessary for the development of TN. Patients with TN without NVC may represent a distinct population of younger, predominantly female patients. Further research into the pathophysiology underlying this debilitating disease is needed.
Immunolocalization and serum antibody responses to Brugia malayi pepsin inhibitor homolog (Bm‐33)

cDNA coding for Brugia malayi pepsin inhibitor homolog (Bm‐33) from the human filarial parasite was cloned in pRSET for large‐scale expression and functional characterization. The pRSET‐B cloned gene did not yield recombinant protein expression and the reason was attributed to the presence of an N‐terminal signal peptide. The gene was subcloned in pRSET‐A without a signal peptide and the 33 kDa histidine‐tagged recombinant protein was purified by IMAC. All individuals from an endemic area generated IgG responses against Bm‐33 in the order MF>CP>EN. Isotype analysis indicated an elevated IgG4 reactivity in the order MF>EN>CP. Bm‐33‐specific IgE levels were elevated in MF, CP and EN compared to non‐endemic normals with no significant differences among the groups. Paraffin‐embedded sections of Setaria digitata (cattle filarial parasite) stained with mouse anti‐Bm‐33 antibodies exhibited the hypodermal nature of Bm‐33. These findings suggest that Bm‐33 is an immunodominant antigen and contributes to filarial pathogenesis.
Phenylketonuria mutations in Europe

Phenylketonuria (PKU) is heterogeneous. More than 400 different mutations in the phenylalanine hydroxylase (PAH) gene have been identified. In a systematic review of the molecular genetics of PKU in Europe we identified 29 mutations that may be regarded as prevalent in European populations. Comprehensive regional data for these mutations were collated from all available studies. The spectrum of mutations found in individual regions results from a combination of factors including founder effect, range expansion and migration, genetic drift, and probably heterozygote advantage. Common mutations include R408W on a haplotype 2 background in Eastern Europe, IVS10–11G>A in the Mediterranean, IVS12+1G>A in Denmark and England, Y414C in Scandinavia, I65T in Western Europe, and R408W on haplotype 1 in the British Isles. Molecular data from mild hyperphenylalaninemia (MHP) patients are available from a number of countries, but it is currently not possible to calculate relative allele frequencies. The available data on PAH mutations are useful for the understanding of both the clinical features and the population genetics of PAH deficiency in Europe. Hum Mutat 21:345–356, 2003. © 2003 Wiley‐Liss, Inc.
A stereological evaluation of secretin and gastric inhibitory peptide‐containing mucosal cells of the perinatal small intestine of the pig

Stereological methods were used to quantify secretin and gastric inhibitory peptide (GIP)‐immunoreactivity (GIP‐IR) in paraffin sections of the duodenum, jejunum and ileum of fetal and neonatal piglets. In addition, sections were processed for GLP‐1‐immunohistochemistry. The volume density of the tunica mucosa increased after birth, giving rise to a decreased volume density of the tela submucosa and tunica muscularis. Generally known region‐specific morphological distinctions were reflected in differing volume densities of the various layers. The highest volume density of GIP‐IR epithelial cells was observed in the jejunum of the neonate. In contrast, the volume density of secretin‐IR epithelial cells was highest in the duodenum of both fetal and neonatal piglets. The volume occupied by GIP‐IR and secretin‐IR epithelial cells increased in the jejunum after birth. Additionally, ileal secretin‐IR epithelial cells were more numerous in the neonatal piglet. In conclusion, the quantitative and qualitative presence of GIP‐IR and secretin‐IR epithelial cells agree with earlier reports of their presence and co‐localization between GIP‐IR and GLP‐1‐IR, in the pig small intestine. Furthermore, the differences suggest that age‐ and region‐related functional demands are temporally and probably causally related with the morphological diversification of the intestine and its endocrine cells.
Nurse practitioner-physician comanagement of primary care patients: The promise of a new delivery care model to improve quality of care.

BACKGROUND The U.S. primary care system is under tremendous strain to deliver care to an increased volume of patients with a concurrent primary care physician shortage. Nurse practitioner (NP)-physician comanagement of primary care patients has been proposed by some policy makers to help alleviate this strain. To date, no collective evidence demonstrates the effects of NP-physician comanagement in primary care. PURPOSE This is the first review to synthesize all available studies that compare the effects of NP-physician comanagement to an individual physician managing primary care. METHODS The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) framework guided the conduct of this systematic review. Five electronic databases were searched. Titles, abstracts, and full texts were reviewed, and inclusion/exclusion criteria were applied to narrow search results to eligible studies. Quality appraisal was performed using Downs and Black's quality checklist for randomized and nonrandomized studies. RESULTS Six studies were identified for synthesis. Three outcome categories emerged: (a) primary care provider adherence to recommended care guidelines, (b) empirical changes in clinical patient outcomes, and (c) patient/caregiver quality of life. Significantly more recommended care guidelines were completed with NP-physician comanagement. There was variability of clinical patient outcomes with some findings favoring the comanagement model. Limited differences in patient quality of life were found. Across all studies, the NP-physician comanagementcare delivery model was determined to produce no detrimental effect on measured outcomes and, in some cases, was more beneficial in reaching practice and clinical targets. PRACTICE IMPLICATIONS The use of NP-physician comanagement of primary care patients is a promising delivery care model to improve the quality of care delivery and alleviate organizational strain given the current demands of increased patient panel sizes and primary care physician shortages. Future research should focus on NP-physician interactions and processes to isolate the attributes of a successful NP-physician comanagement model.
Recent evidence for adverse effects of residential proximity to traffic sources on asthma

Purpose of review A growing body of evidence indicates that residential proximity to traffic sources increases the risk for asthma and asthma exacerbations. In this review we have considered publications from 2006–2007 that examined the impact of residential traffic-related exposures on asthma occurrence and severity. Recent findings In these studies, exposures were estimated using traffic metrics based on residential distances from major roads and freeways, traffic densities around homes, and models of traffic exposure. Overall, residential proximity to traffic sources was associated with increased asthma occurrence and exacerbations in both children and adults. Land-use regression models were superior to individual traffic metrics in explaining the variability of traffic-related pollutants. Susceptibility may also play a role in variation in the effects of traffic on asthma. Summary There is consistent evidence that living near traffic sources is associated with asthma occurrence and exacerbations. Future studies have the opportunity to improve exposure estimates by measuring traffic-related pollutants near homes and schools and including time/activity patterns in prediction models. Further research is also warranted to investigate the differential impact of traffic by genetic and other susceptibility factors and to identify specific pollutants that underlie the adverse effect of traffic on asthma.
Variation of the glycosylation of human pancreatic bile-salt-dependent lipase.

Glycoproteins of human pancreatic juice were characterized by means of lectins after electrophoresis and electrotransfer to nitrocellulose membranes. For the detected glycoproteins, only a 100-kDa glycoprotein varied in the pancreatic juice from a normal patient (i.e. without any pancreatic disorder) compared to the pancreatic juice from a patient suffering from chronic pancreatitis. This protein, which is the only protein in human pancreatic juice which is O-glycosylated and N-glycosylated, was identified as the bile-salt-dependent lipase. Among the glycosylated proteins present in human pancreatic juice, only the glycosylation of bile-salt-dependent lipase differs between individuals. The enzyme was isolated either from normal or pathological human pancreatic juices. The purified variants have an identical molecular mass and amino-acid composition. As suspected from lectin affinity studies, the oligosaccharide composition differs between the variants. The structure of the N-linked oligosaccharides of the variant from the pancreatic juice of a normal donor correlated with complete processing and maturation of a complex-type N-glycan. Alteration of the maturation process can be detected for a bile-salt-dependent-lipase variant from a patient suffering with chronic pancreatitis, since the carbohydrate composition is compatible with the predominance of hybrid or high-mannose-type structures. The amount of sugar involved in O-glycosylation associated with the peanut agglutinin reactivity suggests the presence of 12-14 minimal Gal beta 1-->3GalNac-->T/S O-glycan structures which are sialylated and fucosylated. The amount of sugar involved in the O-linked oligosaccharide structure appears to be unchanged in the variants isolated from the pathological pancreatic juice.
Somatic genetic alterations (loh) in benign, borderline and invasive ovarian tumours: Intratumoral molecular heterogeneity

Loss of heterozygosity (LOH) affects a number of chromosome regions in ovarian cancer, pointing to the possible involvement of tumour‐suppressor genes in ovarian tumorigenesis. We performed comparative analysis of allelic loss at 6 frequently affected chromosome regions in a panel of 53 benign, borderline and malignant ovarian tumours. Precursor lesions could provide evidence that an accumulation of genetic events is required for normal ovarian epithelium to generate malignant tumours. LOH on chromosome 1p was relatively common in benign, borderline and malignant tumours, while at 11p and 7q it was observed not only in invasive but also in borderline tumours. Moreover, 17q and 18q were affected mainly in advanced malignant tumours and revealed a high frequency of clonal intratumoral heterogeneity. We encountered different spectra of genetic alterations in primary tumours and their metastasis, which may be the results of intratumoral heterogeneity leading to dissemination in only some sub‐clones. Int. J. Cancer 82:822–826, 1999. © 1999 Wiley‐Liss, Inc.
Confinement effects on glass forming liquids probed by dynamic mechanical analysis

Many molecular glass forming liquids show a shift of the glass transition ${T}_{g}$ to lower temperatures when the liquid is confined into mesoporous host matrices. Two contrary explanations for this effect are given in literature: First, confinement induced acceleration of the dynamics of the molecules leads to an effective downshift of ${T}_{g}$ increasing with decreasing pore size. Second, due to thermal mismatch between the liquid and the surrounding host matrix, negative pressure develops inside the pores with decreasing temperature, which also shifts ${T}_{g}$ to lower temperatures. Here we present dynamic mechanical analysis measurements of the glass forming liquid salol in Vycor and Gelsil with pore sizes of $d=2.6$, 5.0 and 7.5 nm. The dynamic complex elastic susceptibility data can be consistently described with the assumption of two relaxation processes inside the pores: A surface induced slowed down relaxation due to interaction with rough pore interfaces and a second relaxation within the core of the pores. This core relaxation time is reduced with decreasing pore size $d$, leading to a downshift of ${T}_{g}\ensuremath{\propto}1/d$ in perfect agreement with recent differential scanning calorimetry (DSC) measurements. Thermal expansion measurements of empty and salol filled mesoporous samples revealed that the contribution of negative pressure to the downshift of ${T}_{g}$ is small $(l30%)$ and the main effect is due to the suppression of dynamically correlated regions of size $\ensuremath{\xi}$ when the pore size $d$ approaches $\ensuremath{\xi}$.
20-nm magnetic domain wall motion memory with ultralow-power operation

We study the write and retention properties of magnetic domain wall (DW)-motion memory devices with the dimensions down to 20 nm. We find that the write current and time are scaled along with device size while sufficient thermal stability and low error rate are maintained. As a result, ultralow-power (a few fJ) and reliable operation is possible even at reduced dimensions.
Prognostic factors for musculoskeletal sickness absence and return to work among welders and metal workers.

OBJECTIVES: To analyse factors that determine the occurrence of sickness absence due to musculoskeletal problems and the time it takes to return to work. METHODS: A longitudinal study with two year follow up was conducted among 283 male welders and metal workers. The survey started with a standardised interview on the occurrence of musculoskeletal complaints. 61 (22%) workers were lost to follow up. Data on sickness absence among 222 workers during the follow up were collected from absence records and self reports. Regression analysis based on proportional hazards models was applied to identify risk factors for the occurrence and duration of sickness absence due to various musculoskeletal complaints. RESULTS: During the follow up 51% of the workers attributed at least one period of sickness absence to musculoskeletal complaints which accounted for 44% of all work days lost. A history of back pain was not associated with sickness absence for back pain, partly because subjects with back pain were more likely to be lost to follow up. Neck or shoulder pain and pain of the upper extremities contributed significantly to neck or shoulder absence (relative risk (RR) 3.35; 95% confidence interval (95% CI) 1.73 to 6.47) and to upper extremities absence (RR 2.29; 95% CI 1.17 to 4.46), respectively. Company and job title were also significant predictors for sickness absence due to these musculoskeletal complaints. Absence with musculoskeletal complaints was not associated with age, height, body mass index, smoking, and duration of employment. Return to work after neck or shoulder absence was worse among metal workers than welders (RR 2.12; 95% CI 1.08 to 4.17). Return to work after lower extremities absence was strongly influenced by visiting a physician (RR 11.31; 95% CI 2.94 to 43.46) and by musculoskeletal comorbidity (RR 2.81; 95% CI 1.18 to 6.73). CONCLUSIONS: Complaints of the neck or shoulder and upper extremities in the 12 months before the study were associated with sickness absence for these complaints during the follow up. Workers with absence due to pain from back, neck or shoulder, upper extremities, or lower extremities were at higher risk of subsequent sickness absence in the next year.
Aetiology Based Diagnosis and Treatment Selection in Intellectually Disabled People with Challenging Behaviours

Since both intellectual disability and challenging behaviour are entities encompassing heterogeneous clinical conditions and current taxonomies are of limited use in this field of psychiatry, diagnosing psychiatric symptoms in intellectually disabled patients is still very complex. In the diagnostic process of psychiatric symptoms and behavioural abnormalities, the first step should be genome profiling using the latest techniques in order to detect pathogenic CNVs or single gene mutations that are causative for the developmental delay. Their importance can be derived from the scientific observation that several genetic syndromes are associated with a specific behavioural, psychiatric, neuropsychological or neurological symptom profile, relevant for both choice of treatment and prognosis. Second, it has to be stressed that psychiatric disorders, especially from the depression and anxiety spectrum, frequently manifest with atypical symptoms that may hamper adequate pharmacological treatment. With respect to challenging behaviours in general, it should be emphasized that these are essentially dependent on contextual variables for which no rational pharmacological treatment is available and behavioural interventions are primarily warranted. Prescription of psychotropics has been demonstrated to be marginally effective only and to induce regularly unwanted side effects or even an increase of abnormal behaviours. It is therefore recommended to measure always the plasma concentration of psychotropics and antiepileptics and to perform, preferably prior to the start of treatment, genotyping of relevant cytochrome isoenzymes. In is concluded that, apart from the a priori genetic analysis, careful investigation of the here described data sources is needed to formulate a diagnostic hypothesis and treatment proposal.
Association between posterior uveal melanoma and iris freckles, iris naevi, and choroidal naevi

Aim: To investigate the association between posterior uveal melanoma and iris freckles, iris naevi, and choroidal naevi. Methods: Cross sectional study of 65 patients with posterior uveal melanoma and 218 controls. Iris colour, iris freckles, iris naevi, and choroidal naevi were recorded for each eye of each patient. Results: Iris freckles were present in 40 (61.5%) patients with melanoma and 135 (61.9%) controls (p = 0.494). Iris naevi were present in four (6.2%) patients with melanoma and nine (4.1%) controls (p = 0.955). Choroidal naevi were present in 12 (18.5%) patients with melanoma and 38 (17.4%) controls (p = 0.815). Conclusion: This study did not detect an association between posterior uveal melanoma and iris freckles, iris naevi, or choroidal naevi.