Miracle-dz/UniFAR-FTUs huggingface dataset:
Development and characterization of a novel monoclonal antibody (mNI‐11) that induces cell adhesion of the LPS‐stimulated human monocyte‐like cell line U937

A monoclonal immunoglobulin G1 (IgG1) antibody (mAb), designated mNI‐11, was produced by immunizing mice with the lipopolysaccharide (LPS)‐stimulated monocyte‐like cell line U937. The reactivity of mNI‐11 was tested by the indirect immunofluorescence method. The antigen defined by mNI‐11 was found to be expressed on U937 cells, LPS‐stimulated U937 cells, normal CD14+ cells (monocytes/macrophages), and human umbilical vein endothelial cells (HUVECs). Expression of the antigen defined by mNI‐11 on HUVECs slightly increased in response to exposure to tumor necrosis factor‐α (TNF‐α) and phorbol myristate acetate (PMA). When the reactivity of mNI‐11 and mAbs binding human differentiation antigens such as CD11a, CD11b, CD11c, CD14, CD16, CD18, CD23, CD28, CD29, CD31, CD43, CD44, CD45RA, CD49d, CD50, CD54, CD58, CD80, CD102, CD106, HLA‐class I, or HLA‐class II antigen was compared, no mNI‐11 reactivity resembling that of these mAbs was found. mNI‐11 markedly induced homotypic cell aggregation of U937 cells when they were stimulated with LPS. The mNI‐11‐induced aggregation of LPS‐stimulated U937 cells, referred to as LPS‐U937 cells, required neither Fc receptor engagement nor cross‐linking of the antigen defined by mNI‐11 because aggregation was induced by both F(ab')2 fragments and monovalent F(ab/) fragments of mNI‐11. The mNI‐11‐induced aggregation was blocked by the addition of ethylenediaminetetraacetate, and also when incubated at 4°. mAbs to CD11a/CD18 (lymphocyte‐function associated antigen‐1; LFA‐1) and CD54 (intercellular adhesion molecule‐1; ICAM‐1) completely blocked the LPS‐U937 cell aggregation induced by mNI‐11. The IPS‐U937 cell aggregation induced by mNI‐11 was partially but not completely blocked by the protein kinase C inhibitors sphingosine and H‐7, and was completely blocked by the protein‐tyrosine kinase inhibitor genistein. Interestingly, mNI‐11 markedly promoted LPS‐U937 cell adhesion to HUVECs. The mNI‐11‐induced LPS‐U937 cell adhesion to HUVECs was not reduced in the presence of LFA‐1 (CD11a/CD18) or ICAM‐1 (CD54) mAbs. On the other hand, IPS‐U937 cells, whether treated with mNI‐11 or not, sufficiently adhered to the extracellular matrix protein fibronectin, but not to laminin or collagen type I. However, mNI‐11 did not markedly promote IPS‐U937 cell adhesion to fibronectin. Adhesion of IPS‐U937 cells treated with mNI‐11 to fibronectin was completely blocked by CD29 (β chain of very late antigens) mAb. The surface antigen recognized by mNI‐11 had a molecular size of ~97 kDa under non‐reducing conditions and ~117 kDa under reducing conditions, as determined by immunoblotting analysis. We found that mNI‐11 recognizes an adhesion‐associated molecule distinct from any previously reported in terms of its pattern of cellular distribution and molecular weight, and also found that mNI‐11 has activity which induces cell adhesion/aggregation of U937 cells when stimulated with LPS.
Relative Efficacy of Different Tilts with Biphasic Defibrillation in Humans

Objective: The goal of this study was to assess if tilt bears any impact on defibrillation efficacy of biphasic shocks. Background: Although it has been shown that hiphasic waveform may increase the defibrillation efficacy, this pulsing method has not been as extensively studied in patients, and information regarding the effect of different tilts is lacking. Methods: This study consisted of two similar but distinct protocols including 33 patients undergoing transvenous defibriilator implant. In 17 patients (Part I) defibrillation threshold was obtained delivering biphasic waveforms with 50%, 65%, and 80% tilt in random fashion. Similarly, in 16 patients (Part II) testing of biphasic waveform with 40%, 50%, and 65% tilt was performed in random order. The electrode system used consisted of two transvenous leads and a subcutaneous patch in all 33 patients. Results: In Part I, tilt of 50% demonstrated a defibrillation threshold significantly lower than 65% tilt (7.5 ± 4.3 J vs 9.7 ± 5.0 J; P = 0.04) and 80% tilt (7.5 ± 4.3) vs 11.7 ± 5.9 J; P < 0.01). Similarly, 65% tilt provided a lower defibrillation threshold than 80% tilt (9.7 ± 5.0 J vs 11.7 ± 5.9 J; P = 0.02). In Part II, no significant difference was observed in terms of defibriilation threshold between 40% tilt and the two tilts of 50% and 65%. However, as in Part I, 50% tilt provided a significant reduction of the energy to defibrillate as compared to 65% tilt (6.3 ± 3.6 J vs 9.0 ± 4.8 J; P < 0.01). The 50% tilt resulted in better defibrillation efficacy than 65% tilt independent of the lead system used for testing (Medtronic Transvene and CPI Endotak‐C). Conclusions: Biphasic shocks with 50% tilt required less energy for defibrillation than 40%, 65%, and 80% tilts. However, in the clinical setting a programmable tilt may be preferable to account for some patient‐to‐patient variability.
Multicopy suppression of a gacA mutation by the infC operon in Pseudomonas fluorescens CHA0: competition with the global translational regulator RsmA.

The gacA gene of the biocontrol strain Pseudomonas fluorescens CHA0 codes for a response regulator which, together with the sensor kinase GacS (=LemA), is required for the production of exoenzymes and secondary metabolites involved in biocontrol, including hydrogen cyanide (HCN). A gacA multicopy suppressor was isolated from a cosmid library of strain CHA0 and identified as the infC-rpmI-rplT operon, which encodes the translation initiation factor IF3 and the ribosomal proteins L35 and L20. The efficiency of suppression was about 30%, as determined by the use of a GacA-controlled reporter construct, i.e. a translational hcnA'-'lacZ fusion. Overexpression of the rsmA gene (coding for a global translational repressor) reversed the suppressive effect of the amplified infC operon. This finding suggests that some product(s) of the infC operon can compete with RsmA at the level of translation in P. fluorescens CHA0 and that important biocontrol traits can be regulated at this level.
Boosting Attachment Security to Promote Mental Health, Prosocial Values, and Inter-Group Tolerance

In this article, we conceptualize the sense of attachment security as an inner resource and present theory and research on the broaden and build cycle of attachment security generated by the actual or symbolic encounter with external or internalized loving and caring relationship partners. We also propose that the body of research stimulated by attachment theory offers productive hints about interventions that might increase positive experiences and prosocial behavior by bolstering a person's sense of security. On this basis, we review recent experimental studies showing how interventions designed to increase attachment security have beneficial effects on mental health, prosocial behavior, and intergroup relations, and discuss unaddressed issues concerning the mechanism underlying the beneficial effects of these interventions, the temporal course of these effects, and their interaction with countervailing forces.
Patients' experiences of learning clean intermittent self-catheterization: a qualitative study.

AIM This paper is a report of a study to explore the experiences of learning to carry out clean intermittent self-catheterization and user views of service provision. BACKGROUND This treatment imposes a variety of challenges to users, both physical and emotional. Safety, infection rates and complications have been investigated, but the practicalities of learning and performing self-catheterization have not been addressed. What is currently known about the topic from a user perspective is based on audit, small projects or anecdotal reports provided by continence advisers. METHOD In-depth interviews were carried out in 2006 with eight men and seven women, aged 33-81 years (median 65), and carrying out self-catheterization for a variety of reasons. A grounded theory framework was used. FINDINGS Themes identified were psychological issues, physical problems and service interaction. The communication skills of nurses helped facilitate the learning experience. In conjunction with nurses' skills, a friendly relaxed approach alleviated embarrassment and anxiety, thus facilitating information exchange and retention of information. CONCLUSION Adequate information and thorough instruction by healthcare professionals helps empower people to take control and master the treatment, with potential contribution to ongoing compliance and long-term urinary tract health. Development of a policy supporting evidence-based care and a consistent teaching programme is highly recommended for use where this treatment is regularly employed.
Relationship Between Intangible Assets and Cash Flows: An Empirical Analysis of Publicly Listed Corporations in the Philippines

Businesses have recognized the importance of intangible assets in improving their financial performance, creating value, and maintaining competitiveness. Yet, a number of corporate entities have remained indifferent about investing on intangibles and recognizing their economic benefits. This study examined the economic benefits derived by 140 (out of 264) publicly listed corporations in the Philippines with intangible assets shown on their financial statements from 2010 to 2013. Using the Ordinary Least Square Cross-Section Regression Method and panel regression, results showed that intangible assets impact significantly on total cash flow (91.07%) and cash flow from investing activities (68.13%), operating activities (29.56%), and financing activities (6.07%). Based on z-scores, the study noted that the impact of intangible assets on cash flow is significantly different across these sectors: financial; holding companies; services; and industrial, mining, and oil. Essentially, businesses have to consider the specific sector where they belong in coming up with sound business decisions pertaining to intangible assets.
Comparison of three methods for the estimation of cross-shock electric potential using Cluster data

Abstract. Cluster four point measurements provide a comprehensive dataset for the separation of temporal and spatial variations, which is crucial for the calculation of the cross shock electrostatic potential using electric field measurements. While Cluster is probably the most suited among present and past spacecraft missions to provide such a separation at the terrestrial bow shock, it is far from ideal for a study of the cross shock potential, since only 2 components of the electric field are measured in the spacecraft spin plane. The present paper is devoted to the comparison of 3 different techniques that can be used to estimate the potential with this limitation. The first technique is the estimate taking only into account the projection of the measured components onto the shock normal. The second uses the ideal MHD condition E · B = 0 to estimate the third electric field component. The last method is based on the structure of the electric field in the Normal Incidence Frame (NIF) for which only the potential component along the shock normal and the motional electric field exist. All 3 approaches are used to estimate the potential for a single crossing of the terrestrial bow shock that took place on the 31 March 2001. Surprisingly all three methods lead to the same order of magnitude for the cross shock potential. It is argued that the third method must lead to more reliable results. The effect of the shock normal inaccuracy is investigated for this particular shock crossing. The resulting electrostatic potential appears too high in comparison with the theoretical results for low Mach number shocks. This shows the variability of the potential, interpreted in the frame of the non-stationary shock model.
Politics and Post-Colonial Ideology: Historical Negation and Symbolic Exclusion Predict Political Party Preference

The Dark Duo Model of Post-Colonial Ideology proposes that two core ideologies (Historical Negation and Symbolic Exclusion) play a key role in shaping intergroup relations in post-colonial society. Using a longitudinal panel study of New Zealanders (N = 3,769), we examined the effects of these ideologies on political party preferences from 2009 to 2010. Historical Negation (denying the relevance of historical injustice) and Symbolic Exclusion (discounting indigenous peoples as representative of the national category) uniquely predicted cross-lagged changes in support for conservative (versus liberal) political parties. Testing these models in reverse showed that political party support also predicted the uptake of these ideologies. These effects held after controlling for a host of demographic factors, including socio-economic status, gender, ethnicity, religious affiliation, employment and education. We argue that Historical Negation and Symbolic Exclusion legitimize social inequality in post-colonial societies by shaping political party support, and that change in political support also leads to the endorsement or rejection of these ideologies over time. These findings demonstrate the importance of considering culture-specific ideologies when predicting changes in political attitudes and voting behaviour.
Clinical outcomes of CyberKnife stereotactic radiosurgery for elderly patients with presumed primary stage I lung cancer.

BACKGROUND In certain situations, especially in the elderly patient population, a tissue diagnosis of a suspected pulmonary neoplasm is not feasible. Often, a definitive treatment such as stereotactic body radiosurgery is recommended, rather than active surveillance. The aim of this study is to evaluate the efficacy and tolerability of stereotactic body radiotherapy (SBRT) for elderly patients with presumed primary stage I lung cancer without pathological tissue confirmation. METHODS We performed a retrospective analysis of 25 elderly patients (≥75 years) with presumed primary stage I lung cancer treated with SBRT from 2009-2015. The primary end point was local control (LC); secondary end points were survival and toxicity. RESULTS The median follow-up (FU) was 36.0 months (range, 4 to 84 months). The 1-year LC rate was 100%, 3-year LC rate was 78.8%, and 5-year LC rate was 65.7%. The median progression-free survival (PFS) time was 48.0 months (95% CI: 31.2-64.8). The 1-, 3-, and 5-year overall survival (OS) rates were 96.0%, 70.2%, and 50.7%, respectively. The 1-, 3-, and 5-year cancer-specific survival (CSS) rates were 100%, 81.3%, and 67.0%, respectively. No grade 4 or higher toxicity was encountered. CONCLUSIONS SBRT is safe and effective treatment for patients with presumed primary stage I lung cancer where obtaining pathological confirmation of malignancy is challenging.
Maternal depression and developmental outcome of toddlers and preschoolers

Maternal depression is considered a risk factor for the socioemotional and cognitive development of children [1]. The current prevalence of depression in Canada averages at 6%, which is similar to the rates in other western countries [2] (the female-to-male ratio average is 2:1 [3]). However, the prevalence of postpartum depression is approximately 13% [4]. Women of childbearing age are particularly at risk for depression, and many of them experience high levels of social morbidity and depressive symptoms that are often unrecognized and untreated. Mothers already at risk for depression are particularly fragile during the first months postpartum. Maternal depression has consequences on the child’s development. Because physicians who care for infants and children encounter mothers repeatedly, it is important that they have the knowledge and skills for the detection of symptoms of maternal depression.
NF-κB as a Therapeutic Target in Multiple Myeloma*

We have shown that thalidomide (Thal) and its immunomodulatory derivatives (IMiDs), proteasome inhibitor PS-341, and As2O3 act directly on multiple myeloma (MM) cells and in the bone marrow (BM) milieu to overcome drug resistance. Although Thal/IMiDs, PS-341, and As2O3 inhibit nuclear factor (NF)-κB activation, they also have multiple and varied other actions. In this study, we therefore specifically address the role of NF-κB blockade in mediating anti-MM activity. To characterize the effect of specific NF-κB blockade on MM cell growth and survival in vitro, we used an IκB kinase (IKK) inhibitor (PS-1145). Our studies demonstrate that PS-1145 and PS-341 block TNFα-induced NF-κB activation in a dose- and time-dependent fashion in MM cells through inhibition of IκBα phosphorylation and degradation of IκBα, respectively. Dexamethasone (Dex), which up-regulates IκBα protein, enhances blockade of NF-κB activation by PS-1145. Moreover, PS-1145 blocks the protective effect of IL-6 against Dex-induced apotosis. TNFα-induced intracellular adhesion molecule (ICAM)-1 expression on both RPMI8226 and MM.1S cells is also inhibited by PS-1145. Moreover, PS-1145 inhibits both IL-6 secretion from BMSCs triggered by MM cell adhesion and proliferation of MM cells adherent to BMSCs. However, in contrast to PS-341, PS-1145 only partially (20–50%) inhibits MM cell proliferation, suggesting that NF-κB blockade cannot account for all of the anti-MM activity of PS-341. Importantly, however, TNFα induces MM cell toxicity in the presence of PS-1145. These studies demonstrate that specific targeting of NF-κB can overcome the growth and survival advantage conferred both by tumor cell binding to BMSCs and cytokine secretion in the BM milieu. Furthermore, they provide the framework for clinical evaluation of novel MM therapies based upon targeting NF-κB.
Internet-delivered psychotherapy for depression in adults

Purpose of review The rapidly growing number of published research papers attests to the increasing interest in Internet-delivered psychotherapy (iPT). The present article reviews the current status of iPT for the treatment of adults with symptoms of depression. Recent findings Randomized controlled trials have confirmed the efficacy of guided iPT in treating people with diagnosed or elevated symptoms of depression with equivalent results obtained by programs based on cognitive behavioural or problem solving models. With guidance, effect sizes are comparable to those obtained in face-to-face psychotherapy and low-intensity interventions are as effective as those with higher levels of therapist contact. On current evidence, entirely self-guided programs appear to have fewer benefits, but deliver tangible benefits to completers. Summary Recent studies indicate the utility of iPT. Large-scale trials are needed to evaluate optimal strategies for disseminating iPT. Future studies should independently replicate findings and efforts are required to educate patients and health professionals about iPT.
Environmental, developmental, and genetic factors controlling root system architecture

A better understanding of the development and architecture of roots is essential to develop strategies to increase crop yield and optimize agricultural land use. Roots control nutrient and water uptake, provide anchoring and mechanical support and can serve as important storage organs. Root growth and development is under tight genetic control and modulated by developmental cues including plant hormones and the environment. This review focuses on root architecture and its diversity and the role of environment, nutrient, and water as well as plant hormones and their interactions in shaping root architecture.
Two Distinct Gamma-2 Herpesviruses in African Green Monkeys: a Second Gamma-2 Herpesvirus Lineage among Old World Primates?

ABSTRACT Primate gamma-2 herpesviruses (rhadinoviruses) have so far been found in humans (Kaposi's sarcoma-associated herpesvirus [KSHV], also called human herpesvirus 8), macaques (Macaca spp.) (rhesus rhadinovirus [RRV] and retroperitoneal fibromatosis herpesvirus [RFHV]), squirrel monkeys (Saimiri sciureus) (herpesvirus saimiri), and spider monkeys (Ateles spp.) (herpesvirus ateles). Using serological screening and degenerate consensus primer PCR for the viral DNA polymerase gene, we have detected sequences from two distinct gamma-2 herpesviruses, termedChlorocebus rhadinovirus 1 (ChRV1) and ChRV2, in African green monkeys. ChRV1 is more closely related to KSHV and RFHV, whereas ChRV2 is closest to RRV. Our findings suggest the existence of two distinct rhadinovirus lineages, represented by the KSHV/RFHV/ChRV1 group and the RRV/ChRV2 group, respectively, in at least two Old World monkey species. Antibodies to members of the RRV/ChRV2 lineage may cross-react in an immunofluorescence assay for early and late KSHV antigens.
Plasma Changes of Pituitary Protein 7B2 in Chronic Renal Failure Before and After Haemodialysis, Comparisons with Pituitary Hormones and Neuropeptides

Pituitary protein 7B2, is a highly conserved molecule, located in the gonadotrophs and released by luteinising hormone releasing hormone (LHRH), in vitro and in vivo. 7B2-immunoreactivity (7B2-IR) was estimated in patients with chronic renal failure (CRF) before and after haemodialysis. Twenty patients (twelve male, eight female) aged 18-67 (43.9 + 17.7 [SD]) years with CRF were studied before and after haemodialysis. A blood sample was taken from the haemodialysis access site (Cimino fistula) [n=13] or Scribner shunt [n=7]. Haemodialysis was then carried out using a hollow fibre AK 10 dialyser and Gambro control unit. A second blood sample was taken ten minutes after the end of haemodialysis. Pre and post-dialysis blood pressure, pulse and weight were recorded. Plasma creatinine was also recorded before and after haemodialysis. 7B2IR was est imated us ing a sens i t ive radioimmunoassay (RIA). Chromatography was used to verify the expected elution position of 7B2. Mean plasma creatinine fell from 1223 + 52 (645 1500) μmol/l before to 546 + 40 (230-900) μmol/l after haemodialysis (p < 0.001). 7B2 concentrations in 20 patients with CRF before haemodialysis were found to be 123 + 7.4 pmol/l; post-dialysis 7B2 levels were 262 + 19.5 pmol/l, significantly increased compared to those seen during pre-dialysis (p < 0.01). 7B2 levels in patients with CRF correlated positively with plasma creatinine (r = 0.48, p < 0.04). Α representative chromatographic profile of 7B2– IR in plasma of CRF patients eluted at the expected position. 7B2-IR is increased in CRF patients and is further increased after haemodialysis.
Framework of Postpurchase Consumption Incidence and Quantity

Vol. XXXIX (August 2002), 321–335 321 *Pierre Chandon is Assistant Professor of Marketing, INSEAD (e-mail: pierre.chandon@insead.edu). Brian Wansink is Professor of Business Administration, of Nutritional Science, of Advertising, and of Agricultural and Consumer Economics, University of Illinois at Urbana–Champaign, and Professor of Marketing, Wageningen University (e-mail: wansink@ uiuc.edu). This article has benefited from the comments of Gilles Laurent, Bob Meyer, Jim Hess, Steve Hoch, Scott Neslin, Laurence Capron, Vithala Rao, Delphine Manceau, and the three anonymous JMR reviewers. The authors thank Alain Quaghebeur from Secodip for generously providing the data for Study 1 and thank the Fondation HEC, the INSEAD Foundation, FNEGE, the Dartmouth College Scholars Fund, and the Food and Brand Lab at the University of Illinois for their financial support. PIERRE CHANDON and BRIAN WANSINK*