Miracle-dz/UniFAR-FTUs huggingface dataset:
Field and laboratory methods for quantifying brown adipose tissue thermogenesis

Non‐shivering thermogenesis (NST) is a metabolic response to acute cold exposure that involves the liberation of chemical energy through physiological mechanisms that are separate from muscle shivering. Recent research suggests that the metabolic and endocrine action of brown adipose tissue (BAT) may play an important role in adult human NST. Thus, characterizing variation in BAT across human populations is of central importance to human biologists interested in human energetics and cardio‐metabolic health. The gold standard for measuring BAT requires positron emission tomography (PET) and computed tomography (CT)—a technique that is expensive, exposes the participant to radiation, and is inaccessible to researchers working in many regions. Here, the author outline a noninvasive, portable alternative approach to quantifying BAT that modifies the protocols commonly used in PET/CT studies. The method consists of three components: (a) activating BAT thermogenesis using a mild cooling condition; (b) indirectly quantifying BAT thermogenesis by measuring the change in skin temperature where BAT is commonly stored using infrared thermal imaging; and (c) estimating NST by measuring the change in energy expenditure using open‐circuit indirect calorimetry. The development of “field‐friendly” methods will allow human biologists to better characterize population variation in BAT as well as its adaptive and health significance.
The Economics of Setting Auditing Standards

This paper develops a theory of the negotiating positions, or preferences over auditing standards, of the interest groups that may set such standards. Specifically, we consider how the preferences of auditors as standard setters may differ from the preferences of investors. We represent auditing standards by two properties: toughness and vagueness. Toughness is the level of audit effort required by the standards. Vagueness is the uncertainty of audit effort levels that could be considered by different parties as “in compliance” with the standards. Our model predicts that auditors and investors would consider both toughness and vagueness in influencing or setting auditing standards, since preferences over vagueness depend on the level of toughness. We model the market as composed of auditors with different wealth levels and investors with various investment projects and optimal standards will vary across these parties. We show that as a result of such tensions, auditing standards with a degree of vagueness are preferred. By analyzing the economic incentives of different potential standard setters, we help explain how auditing standards have developed in the real world and lay out the foundation for future research on the standards.
Electroacupuncture alleviates morphine-induced hyperalgesia by regulating spinal CB1 receptors and ERK1/2 activity

Electroacupuncture (EA), a traditional Chinese therapeutic technique, is considered an effective method for treating certain painful neuropathies induced by various neuropathological damage. The current study investigated the effect of EA on intrathecal (IT) morphine-induced hyperalgesia (MIH) and examined the hypothesis that activation of cannabinoid receptor 1 (CB1) could enhance the antinociceptive effect of EA on MIH via regulation of the extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway. Using a rat model of IT MIH, mechanical and thermal hyperalgesia were evaluated by an electronic von Frey filament and hotplate at baseline (1 day before IT administration) and at days 1, 3, 5 and 7 after IT administration. Rats received IT normal saline, IT morphine or IT morphine + EA at ST36-GB34. The protein levels of ERK1/2, phosphorylated (p)-ERK1/2 and CB1 in the spinal cord were assayed by western blotting. Furthermore, the effect of IT injection of the CB1 agonist WIN 55,212-2 and the CB1 antagonist SR141716 on the antinociceptive effect of EA in rats with MIH was investigated. Nociceptive behavior and ERK1/2, phosphorylated (p)-ERK1/2 and CB1 protein levels were evaluated as mentioned above. The results revealed that chronic IT injections of morphine induced a significant decrease in mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) accompanied with remarkable upregulation of p-ERK1/2 in the spinal cord, which could be attenuated by EA at the ST36-GB34 acupoints. In the rat model of MIH, IT injection of WIN 55,212-2 combined with EA induced a significant increase in MWT and TWL accompanied with a significant decrease in p-ERK1/2 and a significant increase in CB1 protein level compared with EA alone, while SR141716 induced the opposite results. The present study suggests that EA alleviates hyperalgesia induced by IT injection of morphine partially through the inhibition of ERK1/2 activation. Activation of the CB1 receptor enhances the antinociceptive effect of EA in rats with MIH partly through the regulation of the spinal CB1-ERK1/2 signaling pathway.
Interference Control in Working Memory Is Associated with Ventrolateral Prefrontal Cortex Volume

Goal-irrelevant information may interfere with ongoing task activities if not controlled properly. Evidence suggests that the ability to control interference is connected mainly to the prefrontal cortex (pFC). However, it remains unclear whether gray matter (GM) volume in prefrontal regions influences individual differences in interference control (IC) and if these relationships are affected by aging. Using cross-sectional and longitudinal estimates over a 4- to 5-year period, we examined the relationship between relative IC scores, obtained from a 2-back working memory task, GM volumes, and performance in different cognitive domains. By identifying individuals with either no or high levels of interference, we demonstrated that participants with superior IC had larger volume of the ventrolateral pFC, regardless of participant demographics. The same pattern was observed both at baseline and follow-up. Cross-sectional estimates further showed that interference increased as a function of age, but interference did not change between baseline and follow-up. Similarly, across-sample associations between IC and pFC volume were found in the cross-sectional data, along with no longitudinal change–change relationships. Moreover, relative IC scores could be linked to composite scores of fluid intelligence, indicating that control of interference may relate to performance in expected cognitive domains. These results provide new evidence that a relative IC score can be related to volume of specific and relevant regions within pFC and that this relationship is not modulated by age. This supports a view that the GM volume in these regions plays a role in resisting interference during a working memory task.
Temporal Trends and Clinical Outcomes Associated with Vasopressor and Inotrope Use In The Cardiac Intensive Care Unit.

BACKGROUND The use of norepinephrine may be associated with better outcomes in some patients with shock. We sought to determine whether norepinephrine was associated with lower mortality in unselected cardiac intensive care unit (CICU) patients compared to other vasopressors, and whether patterns of vasopressor and inotrope usage in the CICU have changed over time. METHODS We retrospectively evaluated consecutive adult patients admitted to a tertiary care hospital CICU from January 1, 2007, to December 31, 2015. Vasoactive drug doses were quantified using the peak Vasoactive-Inotropic Score (VIS). Temporal trends were assessed using the Cochran-Armitage trends test and multivariable logistic regression was used to determine predictors of hospital mortality. RESULTS We included 10,004 patients with a mean age of 67 ± 15 years; vasoactive drugs were used in 2,468 (24.7%) patients. Use of norepinephrine increased over time, while dopamine utilization decreased (p < 0.001 for trends). After adjustment for illness severity and other variables, the peak VIS was a predictor of hospital mortality across the entire population (unit OR 1.013, 95% CI 1.009-1.017, p < 0.001) and among patients receiving vasoactive drugs (OR 1.018, 95% CI 1.013-1.022, p < 0.001). Among patients receiving vasoactive drugs, norepinephrine was associated with a lower risk of hospital mortality (OR 0.66, 95% CI 0.49-0.90, p = 0.008) after adjustment for illness severity and peak VIS. CONCLUSIONS Vasoactive drug use in CICU patients has a dose-dependent association with short-term mortality. Use of norepinephrine in CICU patients is associated with decreased odds of death when compared to other vasoactive drugs.
Modelling the dynamics of children’s gross motor coordination

ABSTRACT This study modelled children’s gross motor coordination, investigated sex-differences and identified the effects of fixed and dynamic correlates on motor coordination development. A total of 344 Portuguese children (170 girls), from 6 age cohorts (5 to 9 years of age), were followed consecutively for three years (age range 5 to 11 years) using a mixed-longitudinal cohort design. Birth weight, hand dominance and socioeconomic status (SES) were identified. Gross motor coordination, body mass index, physical fitness (PF) and physical activity (PA) were assessed annually. A sequence of multilevel hierarchical linear models were developed. Model 1 found that age, age2, sex, sex-by-age and sex-by-age2 were significant predictors (p < 0.05) of gross motor coordination. Boys outperformed girls from 6 years of age onwards. Model 2 found a cohort effect (p < 0.05). Model 3 found that right handers were more coordinated (p < 0.05). When the confounders of body mass index, PF and PA were added to the model (Model 4) it was found that boys and girls had parallel trajectories in their gross motor coordination development. In conclusion children with increasing body mass index were less coordinated, while those who were stronger and more agile had steeper trajectories of gross motor coordination with age.
Who wants to be a citizen scientist? Identifying the potential of citizen science and target segments in Switzerland

Driven by the proliferation of digital media, citizen science – the involvement of non-scientists in scientific research – represents one of the most important recent developments in science communication as it brings science and the public closer together. So far, however, citizen science projects have mostly attracted people that are highly educated, mostly male and already have very positive attitudes towards science. Based on nationally representative survey data (N = 1051), our study explores the potential of citizen science in Switzerland. Using regression analysis, we show that attitudes towards science are significant antecedents of respondents’ interest in participating in citizen science – but that gender and education are not. In addition, latent class analysis identifies five segments, representing over one-third of the Swiss population, who are interested in citizen science and could potentially be engaged: ‘Free-Timers’, ‘Senior Sciencephiles’, ‘Young Sciencephiles’, ‘Intrigued Adolescents’ and ‘Fully Employed Parents’. Additional description suggests that previously overlooked segments are best addressed online via YouTube or offline in zoos or botanical gardens. Overall, our analysis suggests that citizen science’s potential is far higher than previous projects were able to realize.
A prospective study

Cardiac surgery induces a significant inflammatory hypermetabolic stress response, resulting in postoperative hyperglycemia in both preoperatively diabetic and nondiabetic patients. Such postoperative hyperglycemia has been associated with adverse outcomes in surgery and postsurgical recovery. Yet, while diabetes is a known risk factor for postoperative hyperglycemia, predictors of postoperative hyperglycemia among nondiabetics in the local Southeast Asian population remain unknown. We aim to investigate the predictors and outcomes associated with hyperglycemia after cardiac surgery among nondiabetics in the local Southeast Asian population. We analyzed data from 1602 nondiabetic adult patients undergoing elective cardiac surgery, from 2008 to 2010 at the 2 main heart centers in Singapore. Nondiabetic patients who developed postoperative hyperglycemia tended to be women, older, more obese, and hypertensive. Higher body mass index (BMI), age, aortic cross-clamp time, and blood transfusion were identified as independent risk factors of postoperative hyperglycemia. Postoperative hyperglycemia was also significantly associated with postoperative cardiac arrhythmias (26.9% vs 15.0%, P< .001), acute kidney injury (30.0% vs 20.1%, P< .001), longer intensive care unit (ICU) stay (46.7±104.1 vs 37.2±76.6hours, P= .044) and longer hospitalization (11.5±12.2 vs 9.6±8.0 days, P< .001). Our study identified aortic cross-clamp time and blood transfusion as independent risk factors of postoperative hyperglycemia after cardiac surgery in nondiabetics. Similar to other studies, higher BMI and age were independent risk factors for postoperative hyperglycemia. Postoperative hyperglycemia was also associated with adverse perioperative outcomes and should thereby be avoided by treating modifiable risk factors identified in this study including reducing blood transfusion and aortic cross-clamp time. Our findings contribute to early risk stratification of nondiabetic patients who are at increased risk of postoperative hyperglycemia. Abbreviations: ADQI= acute dialysis quality initiative, AKIN = acute kidney injury network, AKI = acute kidney injury, ARF= acute renal failure, ACE = angiotensin converting enzyme, ACC = aortic cross-clamp, BMI = body mass index, CPB = cardiopulmonary bypass, CI = confidence interval, CABG = coronary artery bypass graft, DHEA = dehydroepiandrosterone, eGFR = estimated glomerular filtration rate, EuroSCORE = European System for Cardiac Operative Risk Evaluation, Hct = hematocrit, HDU = high dependency Unit, ICU = intensive care unit, IGF-1 = insulin-like growth factor 1, IRB = Institutional Review Board, LVEF = left ventricular ejection fraction, OR = odds ratio, SIRS = systemic inflammatory response syndrome.
Prescription of renin‐angiotensin‐aldosterone system inhibitors (RAASi) and its determinants in patients with advanced CKD under nephrologist care

Renin‐angiotensin‐aldosterone system inhibitors (RAASi) are recommended for chronic kidney disease (CKD) patients. In this study, we describe RAASi prescription patterns in the Chronic Kidney Disease Outcomes and Practice Patterns Study (CKDopps) in Brazil, Germany, France, and the United States (US). 5870 patients (mean age 66‐72 years; congestive heart failure [CHF] in 11%‐19%; diabetes in 43%‐54%; serum potassium ≥5 in 20%‐35%) were included. RAASi prescription was more common in Germany (80%) and France (77%) than Brazil (66%) and the United States (52%), where the prevalence of prescription decreases particularly in patients with CKD stage 5. In the multivariable regression model, RAASi prescription was least common in the United States and more common in patients who were younger, had diabetes, hypertension, or less advanced CKD. In conclusion, RAASi prescription patterns vary by country, and by demographic and clinical characteristics. RAASi appear to be underused, even among patients with strong class‐specific recommendations. Although the reasons for this variation could not be fully identified in this cross‐sectional observation, our data indicate that the risk of hyperkalemia may contribute to the underuse of this class of agents in moderate to advanced CKD.
Comparison between a facemask and mouthpiece on breathing mechanics and gas exchange variables during high-intensity exercise

Abstract Gas-collection masks are used as a comfortable alternative to the traditional mouthpiece and noseclip during exercise testing protocols in human performance laboratories. However, these masks may introduce potential problems which could affect metabolic and ventilatory parameters, including gas leaks and added dead space. Therefore, the purpose of this study was to compare breathing mechanics, gas exchange variables and ratings of perceived breathlessness (RPB) during high-intensity exercise between a mouthpiece and face mask. Fourteen men [⩒O2peak = 55.3 ± 7.3 ml·kg−1·min−1] were recruited to perform 6 min of cycle ergometry (Velotron Pro, RacerMate, Inc., Seattle, WA) at a work rate corresponding to 90% of ⩒O2peak while breathing on either (1) a mouthpiece (Hans Rudolph, KC, KS) with nose clip, or (2) a face mask (7450, Hans Rudolph, KC, KS). The difference in ⩒E between the mouthpiece (156.8 ± 23.3 L/min) and face mask (153.3 ± 21.8 L/min) was not significant (p = 0.534). Similarly, there were no significant differences in breathing mechanics, gas exchange variables or RPB. These data suggest that the facemask can continue to be used interchangeably with the mouthpiece and may even be a more comfortable alternative during high-intensity exercise.
Comprehensive evaluation of total corneal refractive power by ray tracing in predicting corneal power in eyes after small incision lenticule extraction

Purpose To assess the prediction accuracy of four variations of total corneal refractive power (TCRP) by the ray tracing method in determining corneal power in eyes after myopic small incision lenticule extraction (SMILE). Methods Forty eyes of forty patients who had undergone myopic SMILE were enrolled in this prospective study. Manifest refraction and Pentacam HR were performed preoperatively and three months or more postoperatively. Mean keratometry (Km), true net power (TNP), equivalent keratometry readings (EKR) and 4 subtypes of TCRP (pupil centered or apex centered within a ring or a zone)—TCRPpupil,ring, TCRPpupil,zone, TCRPapex,ring and TCRPapex,zone—were recorded and compared to the theoretical postoperative keratometry value using the clinical history method (CHM). Results The only keratometric values that showed no statistically significant differences from the CHM were 4.0 mm and 4.5 mm EKR, 6.0 mm TCRPpupil,zone and TCRPapex,zone. Pearson’s correlation test revealed that 4.0 mm TCRPpupil,zone exhibited the highest correlation coefficient (r = 0.974) followed by TCRPapex,zone 4.0 mm (0.972) and EKR 4.5 mm (0.970). The 95% limits of agreement (LOA) of the 4.0 mm EKR and CHM, the 4.5 mm EKR and CHM, the 6.0 mm TCRPpupil,zone and CHM, the 6.0 mm TCRPapex,zone and CHM were (-1.27 to 1.22 D), (-1.04 to 0.98 D), (-1.39 to 1.08 D) and (-1.38 to 0.96 D), respectively, while the modified 4.0 mm TCRPpupil,zone (TCRPpuil,zone + 0.70 D) and TCRPapex,zone (TCRPapex,zone+0.70 D) yielded the narrowest 95% LOA of (-0.96 to 0.95 D) and (-0.96D, 1.05 D). Conclusions Total corneal refractive power using the ray tracing method could predict corrected corneal power derived from the CHM in eyes following SMILE surgery after simple modification.
Therapeutic effect of c‐Jun N‐terminal kinase inhibition on pancreatic cancer

c‐Jun N‐terminal kinase (JNK) is a member of the mitogen‐activated protein kinase (MAPK) family, and it is reportedly involved in the development of several cancers. However, the role of JNK in pancreatic cancer has not been elucidated. We assessed t he involvement of JNK in the development of pancreatic cancer and investigated the therapeutic effect of JNK inhibitors on this deadly cancer. Small interfering RNAs against JNK or the JNK inhibitor SP600125 were used to examine the role of JNK in cellular proliferation and the cell cycles of pancreatic cancer cell lines. Ptf1acre/+;LSL‐KrasG12D/+;Tgfbr2flox/flox mice were treated with the JNK inhibitor to examine pancreatic histology and survival. The effect of JNK inhibition on tumor angiogenesis was also assessed using cell lines and murine pancreatic cancer specimens. JNK was frequently activated in human and murine pancreatic cancer in vitro and in vivo. Growth of human pancreatic cancer cell lines was suppressed by JNK inhibition through G1 arrest in the cell cycle with decreased cyclin D1 expression. In addition, oncogenic K‐ras expression led to activation of JNK in pancreatic cancer cell lines. Treatment of Ptf1acre/+;LSL‐KrasG12D/+;Tgfbr2flox/flox mice with the JNK inhibitor decreased growth of murine pancreatic cancer and prolonged survival of the mice significantly. Angiogenesis was also decreased by JNK inhibition in vitro and in vivo. In conclusion, activation of JNK promotes development of pancreatic cancer, and JNK may be a potential therapeutic target for pancreatic cancer.
The Submaximal Lateral Shuffle Test: A reliability and sensitivity analysis

ABSTRACT Lateral ankle stability and how it changes in different footwear has been investigated for years. Research, however, has shown a lack of reliability or sensitivity of available methodologies. This study aimed to evaluate the test-retest reliability and sensitivity of a novel lateral stability protocol, the Submaximal Lateral Shuffle Test (SLST). We recruited 11 and 40 participants to assess reliability and sensitivity of the SLST, respectively. Participants performed the SLST in footwear that differed in collar height and upper stiffness. ICC values showed good to excellent reliability in peak ankle angles and moments, ground reaction forces, impulses, stance time, and performance time. Significantly lower peak inversion and adduction angles and lower medio-lateral push off peak forces were found in the high cut shoes compared to the low cut shoes. The medio-lateral landing peak force showed lower forces in the high cut shoes. The smallest worthwhile change indicated meaningful differences in 70.0–82.5% of participants for inversion, adduction, medio-lateral landing peak, and push off peak forces. These results, however, were not systematic such that there was not a consistent direction of the difference for all participants. In conclusion, the SLST is a promising protocol to further investigate lateral stability in footwear.
Neurocomputational mechanisms at play when weighing concerns for extrinsic rewards, moral values, and social image

Humans not only value extrinsic monetary rewards but also their own morality and their image in the eyes of others. Yet violating moral norms is frequent, especially when people know that they are not under scrutiny. When moral values and monetary payoffs are at odds, how does the brain weigh the benefits and costs of moral and monetary payoffs? Here, using a neurocomputational model of decision value (DV) and functional (f)MRI, we investigated whether different brain systems are engaged when deciding whether to earn money by contributing to a “bad cause” and when deciding whether to sacrifice money to contribute to a “good cause,” both when such choices were made privately or in public. Although similar principles of DV computations were used to solve these dilemmas, they engaged 2 distinct valuation systems. When weighing monetary benefits and moral costs, people were willing to trade their moral values in exchange for money, an effect accompanied by DV computation engaging the anterior insula and the lateral prefrontal cortex (PFC). In contrast, weighing monetary costs against compliance with one’s moral values engaged the ventral putamen. Moreover, regardless of the type of dilemma, a brain network including the anterior cingulate cortex (ACC), anterior insula, and the right temporoparietal junction (TJP) was more engaged in public than in private settings. Together, these findings identify how the brain processes three sources of motivation: extrinsic rewards, moral values, and concerns for image.