Miracle-dz/UniFAR-FTUs huggingface dataset:
Inactive Photosystem II Complexes in Leaves : Turnover Rate and Quantitation.

The flash-induced electrochromic shift, measured by the amplitude of the rapid absorbance increase at 518 nanometers (DeltaA518), was used to determine the amount of charge separation within photosystems II and I in spinach (Spinacia oleracea L.) leaves. The recovery time of the reaction centers was determined by comparing the amplitudes of DeltaA518 induced by two flashes separated by a variable time interval. The recovery of the DeltaA518 on the second flash revealed that 20% of the reaction centers exhibited a recovery half-time of 1.7 +/- 0.3 seconds, which is 1000 times slower than normally active reaction centers. Measurements using isolated thylakoid membranes showed that photosystem I constituted 38% of the total number of reaction centers, and that the photosystem I reaction centers were nearly fully active, indicating that the slowly turning over reaction centers were due solely to photosystem II. The results demonstrate that in spinach leaves approximately 32% of the photosystem II complexes are effectively inactive, in that their contribution to energy conversion is negligible. Additional evidence for inactive photosystem II complexes in spinach leaves was provided by fluorescence induction measurements, used to monitor the oxidation kinetics of the primary quinone acceptor of photosystem II, Q(A), after a short flash. The measurements showed that in a fraction of the photosystem II complexes the oxidation of Q(A) (-) was slow, displaying a half-time of 1.5 +/- 0.3 seconds. The kinetics of Q(A) (-) oxidation were virtually identical to the kinetics of the recovery of photosystem II determined from the electrochromic shift. The key difference between active and inactive photosystem II centers is that in the inactive centers the oxidation rate of Q(A) (-) is slow compared to active centers. Measurements of the electrochromic shift in detached leaves from several different species of plants revealed a significant fraction of slowly turning over reaction centers, raising the possibility that reaction centers that are inefficient in energy conversion may be a common feature in plants.
The Inner Workings of the Outer Surface: Skin and Gill Microbiota as Indicators of Changing Gut Health in Yellowtail Kingfish

The mucosal surfaces and associated microbiota of fish are an important primary barrier and provide the first line of defense against potential pathogens. An understanding of the skin and gill microbial assemblages and the factors which drive their composition may provide useful insights into the broad dynamics of fish host–microbial relationships, and may reveal underlying changes in health status. This is particularly pertinent to cultivated systems whereby various stressors may led to conditions (like enteritis) which impinge on productivity. As an economically important species, we assessed whether the outer-surface bacterial communities reflect a change in gut health status of cultivated Yellowtail Kingfish (Seriola lalandi). Active bacterial assemblages were surveyed from RNA extracts from swabs of the skin and gills by constructing Illumina 16S rRNA gene amplicon libraries. Proteobacteria and Bacteroidetes were predominant in both the skin and gills, with enrichment of key β-proteobacteria in the gills (Nitrosomonadales and Ferrovales). Fish exhibiting early stage chronic lymphocytic enteritis comprised markedly different global bacterial assemblages compared to those deemed healthy and exhibiting late stages of the disease. This corresponded to an overall loss of diversity and enrichment of Proteobacteria and Actinobacteria, particularly in the gills. In contrast, bacterial assemblages of fish with late stage enteritis were generally similar to those of healthy individuals, though with some distinct taxa. In conclusion, gut health status is an important factor which defines the skin and gill bacterial assemblages of fish and likely reflects changes in immune states and barrier systems during the early onset of conditions like enteritis. This study represents the first to investigate the microbiota of the outer mucosal surfaces of fish in response to underlying chronic gut enteritis, revealing potential biomarkers for assessing fish health in commercial aquaculture systems.
Intermediate biomarkers of increased risk for colorectal Cancer: Comparison of different methods of analysis and modifications by chemopreventive interventions

Intermediate biomarkers of abnormal cell growth and development have recently been used in chemoprevention trials in attempts to identify the efficacy of chemopreventive agents in human subjects. Measurements carried out include those related to cell proliferation, differentiation, and gene structure and expression in the colon. Among modified patterns of cell proliferation identified by microautoradiographic or immunoperoxidase assays, a characteristic expansion in the size of the proliferative compartment has been observed in normal‐appearing colorectal mucosa of human subjects with diseases increasing cancer risk: the same patterns have been induced by chemical carcinogens in rodents. Moreover, this intermediate biomarker has been modulated by chemopreventive agents in both rodents and humans. Newer intermediate biomarkers being studied for application to human chemoprevention programs include normal and abnormal patterns of expression of mucins, intermediate filaments and cytoskeletal proteins, and the structure and expression of a variety of genes associated with normal and abnormal cell development. The application of these various intermediate biomarkers to chemoprevention studies is increasing the ability of investigators to analyze the efects of novel chemopreventive agents in the colon and in other organs. © 1992 Wiley‐Liss, Inc.
Age at first birth and long-term health in life course perspective: contextualizing the health effects of fertility timing

We contribute to understanding the link between age at first birth (AFB) and subsequent parental health in later life by contextualizing this relationship in terms of the parents’ broader socio-economic, partnership, and parenting circumstances. Using the Household, Income and Labor Dynamics in Australia (HILDA) survey, we analyse the relationship between AFB and general physical health (N = 5,802 women/4,743 men) and number of chronic diseases (N = 4,438 women/3,519 men). Consistent with previous research, our results show that older AFB is related to significantly better general physical health and fewer chronic diseases for both men and women. However, we also find that a) the magnitude of this relationship is reduced for more advantaged persons, and b) the strength of the relationship between AFB and physical health depends on marital history in a gender-specific fashion. Implications for life course and fundamental cause theories, and for further research, are discussed.
Poor correlation of supratherapeutic international normalised ratio and vitamin K‐dependent procoagulant factor levels during warfarin therapy

Patients with a supratherapeutic international normalised ratio (ST‐INR) are at risk for bleeding. ST‐INR is corrected by withholding warfarin therapy and often by supplementing vitamin K or providing vitamin K‐dependent factors; the exact therapeutic decision is based on the extent of the prolonged INR. Currently, ST‐INRs are frequently observed in clinical practice due to the use of sensitive recombinant tissue thromboplastin reagents and automation. However, there are scant data correlating an ST‐INR with various vitamin K‐dependent factors. This prospective cohort study, set in a large tertiary care teaching hospital for the University of Texas Southwestern Medical Center at Dallas, defined the relationship between ST‐INR (>5·0) and measured vitamin K‐dependent procoagulant factors. Prothrombin time, INR and vitamin K‐dependent factors II, VII, IX and X were measured in 78 patients with an INR > 5·0 (ST‐INR) who were on warfarin therapy for more than 2 months. There was no significant relationship between the ST‐INR and levels of important vitamin K‐dependent factors II and X. These data support the recent guidelines that the management of an INR > 5·0 should be driven by the clinical determinants rather than specific INR values per se.
Endogenous Market Structure, Trade Cost Reduction, and Welfare

We show the long-run effects of a trade cost reduction in an oligopolistic industry. If the labor markets are competitive and the products are homogeneous, a lower transportation cost affects neither consumer surplus nor welfare of the importing country under free entry of domestic firms. If the products are imperfect substitutes, a lower transportation cost may reduce consumer surplus and welfare of the importing country. We also show the implications of a domestic labor union, free entry of domestic and foreign firms, integer constraint, and a tariff as the trade cost.
What Makes Psychiatry such a Stressful Profession? A Qualitative Study

Objective: We aimed to elucidate causative and protective factors associated with burnout in New Zealand psychiatrists, to identify signs and symptoms of the burnout syndrome, and to identify any difference in the perspectives of psychiatrists who scored high versus low on the emotional exhaustion (EE) subscale of the Maslach Burnout Inventory (MBI). Method: Twelve participants were randomly selected, six from each group of psychiatrists (high versus low EE). A face-to-face interview based on a semi-structured questionnaire was carried out with each participant. The interviews were recorded and transcribed verbatim. The transcriptions were manually analysed by two independent reviewers using a grounded theory approach for qualitative analysis. Results: Major features of burnout were changes in appearance, behaviour and mood. Causes of burnout identified were external, internal and interpersonal factors, and changing work environment. Protective factors associated with burnout included supportive relationships, extracurricular activities, variety in one's work, a positive attitude towards one's work and high job satisfaction. Conclusions: A preliminary profile of the burnout syndrome was established and has a potential role in the prevention of burnout in psychiatrists. Causative factors associated with burnout were identified and supported previous findings. Novel factors protective against burnout were suggested and require further investigation. No differences were apparent between the two subgroups of psychiatrists (high versus low EE).
Rapid Pacing Results in Changes in Atrial But Not in Ventricular Refractoriness

SCHOONDERWOERD, B.A., et al.: Rapid Pacing Results in Changes in Atrial But Not in Ventricular Refractoriness. It is well known that atrial tachycardia causes atrial electrical remodeling, characterized by shortening of atrial effective refractory periods (AERPs) and loss of physiological adaptation of AERP to rate. However, the nature and time course of changes in ventricular effective refractory periods (VERP) caused by rapid rates are to be established. After being instrumented with epicardial electrodes on both atria and both ventricles nine goats were subjected to 1 week of rapid AV pacing with a rate of 240 beats/min and an AV delay of 100 ms. Pacing was only interrupted for measurement of left and right AERPs and VERPs at three basic cycle lengths (BCL) of 400 ms, 300 ms, and 200 ms during sinus rhythm in the conscious animal. Left and right AERPs decreased at all BCLs, reaching minimum values after 3 days (right AERP at BCL of 400 ms, 96 ± 16 ms after 3 days vs 144 ± 16 ms at baseline, P < 0.05). In contrast, both left and right VERPs did not change at any BCL. This study demonstrates a difference between the atria and ventricles with respect to tachycardia induced changes in refractoriness.
Perennial Grass and Native Wildflowers: A Synergistic Approach to Habitat Management

Marginal agricultural land provides opportunities to diversify landscapes by producing biomass for biofuel, and through floral provisioning that enhances arthropod-mediated ecosystem service delivery. We examined the effects of local spatial context (adjacent to woodland or agriculture) and irrigation (irrigation or no irrigation) on wildflower bloom and visitation by arthropods in a biofeedstocks-wildflower habitat buffer design. Twenty habitat buffer plots were established containing a subplot of Napier grass (Pennisetum perpureum Schumach) for biofeedstock, three commercial wildflower mix subplots, and a control subplot containing spontaneous weeds. Arthropods and flowers were visually observed in quadrats throughout the season. At the end of the season we measured soil nutrients and harvested Napier biomass. We found irrespective of buffer location or irrigation, pollinators were observed more frequently early in the season and on experimental plots with wildflowers than on weeds in the control plots. Natural enemies showed a tendency for being more common on plots adjacent to a wooded border, and were also more commonly observed early in the season. Herbivore visits were infrequent and not significantly influenced by experimental treatments. Napier grass yields were high and typical of first-year yields reported regionally, and were not affected by location context or irrigation. Our results suggest habitat management designs integrating bioenergy crop and floral resources provide marketable biomass and habitat for beneficial arthropods.
Institutional Change, Regional Features and Labour Market Performance in eight EU's Transition Countries

The aim of this paper is to throw some light on the main differences/similarities and dynamics in institutional frameworks, regional/sectoral features and labour market performances in the eight transition countries that became EU members in May 2004 (8-CEECs). In the second section, a partial review of the main theoretical and empirical literature on the "great transformation" (Kornai, 2006) is presented, with a particular attention to the researches focusing on the relationship between institutional change and economic/employment performance and to the studies considering some regional features of the transition processes. Some stylized facts for the eight CEECs are presented in the third section, by distinguishing (i) initial conditions, (ii) institutional changes and progress in transition and (iii) economic/ employment performance (GDP growth, unemployment and employment rates, etc.). In the forth section, the empirical results on some regional (NUTS 3) features (convergence, concentration and specialisation) of the 8-CEECs are discussed. Finally, an attempt to econometrically investigate some determinants of regional income convergence and national GDP and employment dynamics is presented in the fifth section, by highlighting the role of institutional change and some regional features. The main policy implications, concerning both European and national economic policies, are presented in the conclusive section.
GDP growth determinants and foreign direct investment causality: the case of Iran

Given the economic conditions in Iran and the need to accelerate its economic growth, there is a rising interest in examining the variables that fuel its GDP growth. The scant literature on economic growth in Iran is composed of only a few scholarly studies that investigate this nation's GDP growth. However, none of these studies has examined the causality between GDP growth and its determining elements. The purpose of this study is: (1) to determine the economic variables that contribute to Iran's GDP per capita growth over time, and (2) to examine the causality between foreign direct investment and the relevant variables that are included in the model. To achieve these goals the study uses a model that is based on the postulates of de Mello. The results indicate that: (1) the major determinants of GDP per capita growth in Iran are value added growth and domestic investment growth; (2) there is no causal relationship between foreign direct investment growth and GDP per capita growth in Iran in either direction; and (3) there is no causal relationship between foreign direct investment growth and value added growth in Iran in either direction.
The Immune Response to Ocular Herpes Simplex Virus Type 1 Infection

Herpes simplex virus type 1 (HSV-1) is a prevalent microbial pathogen infecting 60% to 90% of the adult world population. The co-evolution of the virus with humans is due, in part, to adaptations that the virus has evolved to aid it in escaping immune surveillance, including the establishment of a latent infection in its human host. A latent infection allows the virus to remain in the host without inducing tissue pathology or eliciting an immune response. During the acute infection or reactivation of latent virus, the immune response is significant, which can ultimately result in corneal blindness or fatal sporadic encephalitis. In fact, HSV-1 is one of the leading causes of infectious corneal blindness in the world as a result of chronic episodes of viral reactivation leading to stromal keratitis and scarring. Significant inroads have been made in identifying key immune mediators that control ocular HSV-1 infection and potentially viral reactivation. Likewise, viral mechanisms associated with immune evasion have also been identified and will be discussed. Lastly, novel therapeutic strategies that are currently under development show promise and will be included in this review. Most investigators have taken full advantage of the murine host as a viable working in vivo model of HSV-1 due to the sensitivity and susceptibility to viral infection, ease of manipulation, and a multitude of developed probes to study changes at the cellular and molecular levels. Therefore, comments in this review will primarily be restricted to those observations pertaining to the mouse model and the assumption (however great) that similar events occur in the human condition.
Relationship of serum resistin level to traits of metabolic syndrome and serum paraoxonase 1 activity in a population with a broad range of body mass index.

UNLABELLED The relationship between resistin, one of the adipokines, and metabolic syndrome is not fully elucidated. Altered activity of the HDL-associated antioxidant enzyme paraoxonase 1 (PON1) that participates in the antioxidant defense mechanisms of HDL may have an important role in the obesity-related accelerated atherosclerosis. Inverse associations of PON1 with obesity and serum levels of leptin have been demonstrated. Our aim was to investigate the association of serum levels of resistin with (i) PON1 activity, and (ii) parameters of metabolic syndrome, including some that are additional for research. A total of 74 Caucasian subjects were recruited into the study and divided into 3 age and sex-matched groups. Group 1, 25 non-diabetic overweight/obese subjects with BMI of 28-39.9 kg/m (2); group 2, 25 non-diabetic obese patients with BMI >or=40 kg/m (2); and the control group 3, 24 healthy, normal-weight control subjects. Serum levels of resistin were correlated negatively with BMI (r=-0.27, P<0.05), waist circumference (r=-0.28, P<0.05), serum levels of leptin (r=-0.28, P<0.05), non-esterified fatty acids (NEFA) (r=-0.23, P<0.05), and HbA (1C) (r=-0.26, P<0.05), systolic BP (r=-0.28, P<0.05), and lipid peroxidation (measured by TBARS) (r=-0.40, P<0.01), and correlated positively with PON1 (r=0.24, P<0.05). No association was detected between the serum concentrations of resistin and the following investigated parameters: diastolic BP, levels of uric acid, glucose, insulin, or insulin resistance (measured by homeostasis model assessment, HOMA-IR), triglyceride, total cholesterol, LDL-C, and HDL-C. During multiple regression analyses BMI and TBARS were independent predictors of PON1, while age, gender, blood pressure, HOMA-IR, LDL-C, HDL-C, and resistin were not. CONCLUSIONS Among the study subjects, serum levels of resistin showed a positive, although not independent correlation with serum PON1, and a negative correlation with numerous parameters of the metabolic syndrome (i.e. adiposity, blood pressure, levels of leptin, free fatty acid, glycosylated hemoglobin, and lipid peroxidation). BMI and TBARS are independent predictors of PON1 activity.
A focus group assessment to determine motivations, barriers and effectiveness of a university-based worksite wellness program.

BACKGROUND This study explores university employee perceptions and under-standing about its Worksite Health Promotion Program (WHPP). The WHPP included a Health Risk Appraisal (HRA), biometric screening, publicity for on-campus health programs and facilities, and health coaching. METHODS A qualitative design was used based on a grounded theory ap-proach. Four 90 minutes focus groups with 6-8 participants in each were conducted within a two 2 week period among employees, representing fac-ulty/participants, fac-ulty/nonparticipants, staff/participants, and staff/nonparticipants. Responses to questions about motivations, barriers, and perceived health benefits that impacted participation in the WHPP were digi-tally recorded, transcribed and coded for themes. RESULTS Incentives effectively motivated participation. Biometric screening had the largest impact on behavior change, followed by the information learned from the HRA. However, despite two-thirds of the employees partici-pating in the pro-gram, lack of a full understanding of WHPP benefits and services lowered partici-pation in follow-up services and supplemental pro-grams. CONCLUSIONS Biometric screening and HRAs effectively motivate program par-ticipation. Communication of benefits and services are important when providing WHPPs.
Predictors of placebo response in peripheral neuropathic pain: insights from pregabalin clinical trials

Background Greater understanding of factors associated with the high placebo-response rates noted in recent neuropathic pain trials may improve trial design. This study investigated placebo response and its predictors in pregabalin trials in patients with diabetic peripheral neuropathy (DPN) or postherpetic neuralgia. Patients and methods Individual patient data from 16 randomized, placebo-controlled, double-blind trials of pregabalin in 3,053 patients with DPN and 1,460 patients with postherpetic neuralgia were pooled (by condition and all together) in order to investigate the placebo response and its predictors. Univariate and multivariate analyses were performed across all 16 trials to identify predictors of change in pain score in patients. Trials with a >2-point mean reduction in pain score at endpoint with placebo were designated high placebo response and were compared with low placebo-response trials (those with a ≤2-point mean reduction) with respect to patient and study characteristics. Results Three high placebo-response studies were identified, with all in DPN patients and all conducted postapproval of pregabalin. Younger age, higher mean baseline pain score, longer study duration, higher ratio of patients on active treatment to placebo, and study conducted postapproval were all significantly associated with a higher placebo response (P<0.05). There was a trend towards an increased placebo response in all studies over time without any corresponding change in the response to pregabalin. Conclusion Consideration of the factors identified here as contributing to a higher placebo response could help improve the sensitivity and accuracy of clinical trials in patients with neuropathic pain.