Miracle-dz/UniFAR-FTUs huggingface dataset:
Subscales of the Fear Survey Schedule-III in Community Dwelling Older Adults – Relations to Clinical and Cognitive Measures

ABSTRACT Objectives: One major obstacle to the diagnosis and treatment of specific phobias in later life is the lack of assessment tools that are relevant to the fears of older adults. This study investigated the utility of five reliable subscales (Blood/Injury, Agoraphobia, Aggression, Animal/Insect, Social Fears) derived from the Fear Survey Schedule-III, a popular measure of phobic fear, in discriminating older from younger participants. Method: The sample was comprised of 81 younger and 61 older adults who completed self-report measures of anxiety and depression and neuropsychological tests. Results: Older adults scored significantly higher than their younger counterparts only on the Aggression subscale of the FSS-III; whereas younger adults scored significantly higher than the older group only on the Social Fears subscale. These subscales showed slightly different associations with other measures of anxiety, depression, and intelligence across age groups. Within the older sample, scores on the Aggression subscale were significantly higher than all other subscales except for Social Fears. Conclusions: It is recommended that clinicians and researchers use subscale scores derived from the FSS-III, rather than total scores, when treating and studying fears of later life. Clinical Implications: Use of FSS-III subscales in assessment could result in improved detection and treatment of phobias in later life, and thus raise quality of life among the elderly. Older adults’ fears of victimization should be prioritized as a possible treatment target in clinical settings.
The Influence of Passive Haptic Feedback and Difference Interaction Metaphors on Presence and Task Performance

This paper explores the influence of passive haptic feedback on presence and task performance using two important interaction metaphors. We compared direct interaction with the user's hand with interaction using a stylus. Twenty-four participants performed a simple selection task consisting of pressing buttons while playing a memory game, with haptic feedback and interaction metaphor as the independent variables. We measured task performance by computing errors and time between button presses. We measured presence with questionnaires and through a new method based on users' involuntary movements. Our results suggest that passive haptic feedback improves both presence and task performance. However, small but significant differences related to the interaction metaphor were only apparent when haptic feedback was not provided.
Impairments in life satisfaction in infertility: Associations with perceived stress, affectivity, partnership quality, social support and the desire to have a child

Abstract Between 10% and 15% of couples in western society are affected by infertility. As a major source of psychological distress, infertility may be accompanied by a substantial decline in well-being, and mental symptoms including those of depression and anxiety. This study aimed to quantify impairments in life satisfaction in infertile couples and to identify psychosocial variables moderating these impairments. For this purpose, 228 infertile couples completed the Life Satisfaction Questionnaire (LSQ), Perceived Stress Questionnaire (PSQ), Positive and Negative Affect Schedule (PANAS), Penn State Worry Questionnaire (PSWQ), Partnership Questionnaire (PQ), Social Support Questionnaire, (SSQ) and Questionnaire on the Desire to Have a Child (QDC). Our sample scored below the norm of the general population on the LSQ sum score and various subscales, including those assessing social relationships, health, sexuality, leisure activities, and self-evaluation. Correlation and regression analysis revealed an inverse association of life satisfaction (LSQ) with stress (PSQ), negative affect (PANAS), and worry (PSWQ), and positive associations with positive affect (PANAS), partnership quality (PQ), social support (SSQ), and the strength of the desire to have a child (QDC). These findings support the notion of markedly reduced satisfaction with numerous life areas in the context of infertility. Psychosocial factors such as increased stress related to infertility treatment, affective impairments, partnership problems, lack of social support or social exclusion may contribute to this reduction. Psychological interventions aimed at controlling stress, strengthening partnerships, and improving social inclusion may be helpful in reducing the burden of infertility and improving the life satisfaction of affected couples.
High prevalence of SCC mec-associated Phenol-soluble modulin gene in clinical isolates of methicillin-resistant. Staphylococcus aureus.

INTRODUCTION We aimed to determine the distribution of Phenol-soluble modulin-mec (psm-mec) gene and its relationship with biofilm formation in clinical methicillin-resistant S. aureus (MRSA). METHODS In a descriptive study, a total of 94 cefoxitin-resistant S. aureus isolates were collected from patients and tested for antibiotic susceptibility testing, multiplex polymerase chain reaction (MPCR) for detection of mecA and pvl genes, PCR for detection of psm-mec gene and SCCmec typing of psm-mec and pvl-positive isolates. Furthermore, isolates were tested by microtiter plate method for biofilm formation assay. RESULTS Multiplex PCR for detection of mecA and pvl genes was performed for all cefoxitin-resistant isolates. The mecA gene was found in 92 (97.9%) isolates but none of the isolates carried the pvl gene. Sixty-five (69.1%) isolates harbored psm-mec genes and 95.4% of these isolates belong to SCCmec type III. Statistical analysis showed a significant difference between the presence or absence of psm-mec gene and biofilm production (P<0.001). CONCLUSION In this study, more than half of the MRSA strains harbored psm-mec gene and almost one-fifth of them produced strong biofilm. Since the strains with strong biofilm formation have more antibiotic resistance and cause the long-lasting infection, for the suitable treatment of hospitalized patients with this kind of MRSA strains, we should be paid more attention to these strains.
Dissociating the neural substrates for inhibition and shifting in domain‐general cognitive control

Inhibition and shifting are two key components of domain‐general cognitive control. Numerous studies have investigated the neural substrates of both components, but it is still unclear whether the relevant brain regions are specifically involved in one specific component or commonly engaged in both components. Here, we addressed this question by using functional magnetic resonance imaging and a modified saccade paradigm that was effective to disentangle inhibition and shifting in one experiment. The results showed that both the middle frontal gyrus and left parietal lobe were involved in both components but the middle frontal gyrus was more active for the inhibition while the inferior parietal lobe was more active for the shifting processing. The outcome suggests that, although both regions are engaged in inhibition and shifting, each plays a dominant role in one component. These findings provide a further insight into the neural dissociation in inhibition and shifting, as well as a better explanation on the framework of unity and diversity from a neuropsychological viewpoint.
The nociceptive withdrawal reflex of the trunk is organized with unique muscle receptive fields and motor strategies

Noxious stimuli induce a nociceptive withdrawal reflex (NWR) to protect the tissue from injury. Although the NWR was once considered as a stereotyped response, previous studies report distinct responses depending on the stimulation site and context for limbs. We aimed to determine whether noxious stimuli over the trunk produced adaptable complex NWR. We hypothesized that organization of the NWR of the trunk muscle would vary with the site of noxious input and would differ between body and spine postures, which modify the potential for specific muscles to remove threat. Fourteen participants were tested in sitting and three lumbar spine postures in side lying (neutral, flexion and extension). Noxious electrical stimuli were applied over the sacrum, spinous process of L3 and T12, lateral side of the 8th rib and anterior midline. NWR latency and amplitude were recorded with surface electromyography (EMG) electrodes over different trunk muscles. Distinct patterns of muscle activation depended on the stimulation site and were consistent with motor strategies needed to withdraw from the noxious stimuli. The NWR pattern differed between body positions, with less modulation observed in sitting than side lying. Spine posture did not affect the NWR organisation. Our results suggest the circuits controlling trunk muscle NWR presents with adaptability as a function of stimulation site and body position by utilizing the great complexity of the trunk muscle system to produce an efficient protective response. This suggests that the central nervous system (CNS) uses multiple adaptable strategies that are unique depending on which context the noxious stimuli are applied.
Impaired Ca2+ Regulation of CD4+CD25+ Regulatory T Cells from Pediatric Asthma

Background: CD4<sup>+</sup>CD25<sup>+</sup> regulatory T (T<sub>reg</sub>) cells can control the allergic response to allergen, airway eosinophilia and airway hypersensitivity. We speculated that chronic inflammation persisting in asthma airways is dependent on abnormalities of these T<sub>reg</sub> cells. There are differences in the pathology of asthma in adults and children, and the airways of pediatric asthma are considered to be more naive than those of adults. Therefore, we analyzed the functionality of T<sub>reg</sub> cells in pediatric asthma and the relationship between T<sub>reg</sub> function and asthma symptoms. Methods: The anergic state, which is one of the defining properties of T<sub>reg</sub>, was analyzed by measuring intracellular Ca<sup>2+</sup> influx following T cell receptor (TCR) stimulation. FOXP3-positive cells and FOXP3 mRNA expression were measured by flow analysis and real-time PCR with the SYBR method, respectively. Results: CD45RO<sup>+</sup> cells make up approximately 99% of CD4<sup>+</sup>CD25<sup>high</sup> T cells and 89% of CD4<sup>+</sup>CD25<sup>low</sup> T cells in human adult blood. The proportion of CD45RO<sup>+</sup> cells in CD4<sup>+</sup>CD25<sup>+</sup> (high + low) T cells from pediatric asthma was much smaller (about 56%). Interestingly, our data indicated that CD45RO<sup>+</sup> T<sub>reg</sub> cells from pediatric asthma aberrantly increased intracellular Ca<sup>2+</sup> concentrations following TCR activation compared with pediatric nonasthma controls. Conclusion: These impaired CD45RO<sup>+</sup> T<sub>reg</sub> cell functions were correlated with asthma symptoms. The correlation was observed in the group with a highly expressed atopic phenotype and longer duration of asthma. We suggest that chronic inflammation in pediatric asthma airways may be the result of impaired regulatory functions of CD45RO<sup>+</sup> T<sub>reg</sub> cells.
New Clinical Nurse Faculty Orientation: A Review of the Literature.

AIM The aim of this literature review was to obtain relevant information and research findings about orientation for new clinical nurse faculty. BACKGROUND Hiring clinical experts as clinical faculty is common practice to address the nurse faculty shortage. As clinical experts do not possess the pedagogical expertise needed for effective teaching, they require specialized orientation when entering a faculty role. There is no consensus as to best practices for new clinical faculty orientation due to a dearth of research in this area. METHOD A comprehensive search of the literature was conducted to obtain relevant information and research findings about orientation for new clinical nursing faculty. RESULTS Current orientation strategies include face-to-face, hybrid, and mentoring components. The limited empirical research is mostly descriptive or pilot studies. DISCUSSION More research is needed on the effectiveness of various forms of orientation for new clinical faculty to support clinical faculty competence, patient safety, and evidence-based practices in this area.
The Role of KRAS in Endometrial Cancer: A Mini-Review

Endometrial cancer (EC) is the most common cancer of the female genital tract, resulting annually in 76,000 related deaths worldwide. EC originates either from oestrogen-related proliferative endometrium (type I, endometrioid), or from atrophic endometrium (type II, non-endometrioid). Each type of EC is characterized by different molecular profile alterations. The Kirsten rat sarcoma viral oncogene homolog (KRAS) gene encodes a signalling protein which moderates response to various extracellular signals via down-regulation of the mitogen-activated protein kinase (MAPK) or phosphoinositide-3-kinase/v-akt murine thymoma viral oncogene (PI3K/AKT) pathways. This article reviews the role of KRAS in predicting transition from hyperplastic endometrium to early-stage well-differentiated EC, as well as further invasive proliferation of the tumour to advanced-stage disease. KRAS seems to be directly associated with type I EC, and most studies support its early involvement in carcinogenesis. Current evidence correlates KRAS mutations with increased cell proliferation and apoptosis, as well as up-regulation of endometrial cell oestrogen receptors. Tumours positive for KRAS mutation can harbour hypermethylation-related changes in genome expression, and this can be the cause of concurrent loss of DNA repair proteins. Despite some evidence that KRAS mutation status affects cancer progression, a consensus is yet to be reached. Based on the available evidence, we suggest that screening for KRAS mutations in patients with hyperplastic endometrium or early-stage type I EC, may provide important information for prognosis stratification, and further provision of personalised treatment options.
Functional characterization of various channel‐expressing central airway epithelial cells from mouse induced pluripotent stem cells

Functional central airway epithelial cells (CAECs) from induced pluripotent stem cells (iPSCs) are an attractive potential cell source for central airway regeneration. The central airway epithelium, such as the tracheal epithelium, is composed of ciliated cells, goblet cells, and basal cells and has physiologically important functions such as the regulation of water volume on the airway surface by Cl− and water channels and the elimination of particles inhaled from the external environment by ciliary movement. Previous work from our group and from other research groups has reported the generation of airway epithelial cells from iPSCs. However, it remains unclear whether iPSC‐derived CAECs express the various channels that are required for the regulation of water volume on the airway surface and whether these channels function properly. In this study, we generated CAECs from iPSCs supplemented with activin and bFGF using air–liquid interface culture. We then evaluated the physiological functioning of the iPSC‐derived CAECs by examining the gene expression and transport functions of Cl − channels using a halide ion‐sensitive yellow fluorescent protein and ciliary movement. Reverse‐transcription polymerase chain reaction and immunohistochemistry indicated that various channel markers such as cystic fibrosis transmembrane conductance regulator (CFTR) and aquaporin (AQP) were present in iPSC‐derived CAECs. Furthermore, the transport functions of Cl − channels and CFTR were successfully confirmed. Finally, ciliary movement was measured, and a ciliary beating frequency (CBF) of approximately 10 Hz was observed. These results demonstrate that CAECs generated by our method have physiological functions similar to those of native CAECs.
Induction of labor at full‐term in pregnant women with uncomplicated singleton pregnancy: A systematic review and meta‐analysis of randomized trials

The lowest incidence of perinatal morbidity and mortality occurs around 39‐40 weeks. Therefore, some have advocated induction of uncomplicated singleton gestations once they reach full‐term. The aim of the study was to evaluate the risk of cesarean delivery, and any maternal and perinatal effects of a policy of induction of labor in women with full‐term uncomplicated singleton gestations.
Identification of three podoviruses infecting Klebsiella encoding capsule depolymerases that digest specific capsular types

Klebsiella pneumoniae is an important human pathogen causing opportunistic nosocomial and community‐acquired infections. A major public health concern regarding K. pneumoniae is the increasing incidence of multidrug‐resistant strains. Here, we isolated three novel Klebsiella bacteriophages, KN1‐1, KN3‐1 and KN4‐1, which infect KN1, KN3 and K56, and KN4 types respectively. We determined their genome sequences and conducted a comparative analysis that revealed a variable region containing capsule depolymerase‐encoding genes. Recombinant depolymerase proteins were produced, and their enzymatic activity and specificity were evaluated. We identified four capsule depolymerases in these phages that could only digest the capsule types of their respective hosts. Our results demonstrate that the activities of these capsule depolymerases were correlated with the host range of each phage; thus, the capsule depolymerases are host specificity determinants. By generating a capsule mutant, we demonstrate that capsule was essential for phage adsorption and infection. Further, capsule depolymerases can enhance bacterial susceptibility to serum killing. The discovery of these phages and depolymerases lays the foundation for the typing of KN1, KN3, KN4 and K56 Klebsiella and could be useful alternative therapeutics for the treatment of K. pneumoniae infections.
Transgenic Expression of IL15 Improves Antiglioma Activity of IL13Rα2-CAR T Cells but Results in Antigen Loss Variants

Glioblastoma responds imperfectly to immunotherapy. Transgenic expression of IL15 in T cells expressing chimeric antigen receptors improved their proliferative capacity, persistence, and cytokine production. The emergence of antigen-loss variants highlights the need to target multiple tumor antigens. Glioblastoma (GBM) is the most aggressive primary brain tumor in adults and is virtually incurable with conventional therapies. Immunotherapy with T cells expressing GBM-specific chimeric antigen receptors (CAR) is an attractive approach to improve outcomes. Although CAR T cells targeting GBM antigens, such as IL13 receptor subunit α2 (IL13Rα2), HER2, and EGFR variant III (EGFRvIII), have had antitumor activity in preclinical models, early-phase clinical testing has demonstrated limited antiglioma activity. Transgenic expression of IL15 is an appealing strategy to enhance CAR T-cell effector function. We tested this approach in our IL13Rα2-positive glioma model in which limited IL13Rα2-CAR T-cell persistence results in recurrence of antigen-positive gliomas. T cells were genetically modified with retroviral vectors encoding IL13Rα2-CARs or IL15 (IL13Rα2-CAR.IL15 T cells). IL13Rα2-CAR.IL15 T cells recognized glioma cells in an antigen-dependent fashion, had greater proliferative capacity, and produced more cytokines after repeated stimulations in comparison with IL13Rα2-CAR T cells. No autonomous IL13Rα2-CAR.IL15 T-cell proliferation was observed; however, IL15 expression increased IL13Rα2-CAR T-cell viability in the absence of exogenous cytokines or antigen. In vivo, IL13Rα2-CAR.IL15 T cells persisted longer and had greater antiglioma activity than IL13Rα2-CAR T cells, resulting in a survival advantage. Gliomas recurring after 40 days after T-cell injection had downregulated IL13Rα2 expression, indicating that antigen loss variants occur in the setting of improved T-cell persistence. Thus, CAR T cells for GBM should not only be genetically modified to improve their proliferation and persistence, but also to target multiple antigens. Summary: Glioblastoma responds imperfectly to immunotherapy. Transgenic expression of IL15 in T cells expressing CARs improved their proliferative capacity, persistence, and cytokine production. The emergence of antigen loss variants highlights the need to target multiple tumor antigens. Cancer Immunol Res; 5(7); 571–81. ©2017 AACR.
THE EFFECTS OF HYPONATREMIA ON BONE DENSITY AND FRACTURES: A SYSTEMATIC REVIEW AND META-ANALYSIS.

Objective: Hyponatremia decreases bone mineral density and is a major risk factor for fragility fractures. Objectives of our systematic review and meta-analysis were to analyze the overall effects of hyponatremia on bone fractures, osteoporosis, and mortality. Methods: We extracted data from Medline, Cochrane Central, and EMBASE 1960-2017 and conference abstracts from 2007-2017. We included studies with data on serum sodium, fractures, bone density, or diagnoses of osteoporosis. Studies were independently reviewed by two authors and assessed for bias using the Newcastle-Ottawa scale. Random effect models meta-analysis was used when at least three studies reported the same outcome measures. We reported summary odds ratios (ORs) and 95% confidence intervals (CIs). Results: We included 26 studies for qualitative analysis. Fifteen studies were included in the meta-analysis to evaluate the effects of hyponatremia on fractures, four studies for bone mineral density changes, and six for mortality. Hyponatremia increased the odds of fractures at all sites (summary OR, 2.34 [95% CI, 1.86, 2.96]. There was an increase in the odds of osteoporosis (summary OR, 2.67 [95% CI, 2.07, 3.43]). Mortality risk among the included studies remained high (summary OR, 1.31 [95% CI, 1.16, 1.47]). Conclusion: Our meta-analysis confirms a statistically significant association of hyponatremia with bone fractures and osteoporosis along with higher mortality. Long-term prospective studies evaluating the impact of correcting hyponatremia on bone health, fractures, and mortality are required. Abbreviations: AVP = arginine vasopressin; CI = confidence interval; CKD = chronic kidney disease; OR = odds ratio; SIADH = syndrome of inappropriate antidiuretic hormone.
Embryo responses to stress induced by assisted reproductive technologies

Assisted reproductive technology (ART) has led to the birth of millions of babies. In cattle, thousands of embryos are produced annually. However, since the introduction and widespread use of ART, negative effects on embryos and offspring are starting to emerge. Knowledge so far, mostly provided by animal models, indicates that suboptimal conditions during ART can affect embryo viability and quality, and may induce embryonic stress responses. These stress responses take the form of severe gene expression alterations or modifications in critical epigenetic marks established during early developmental stages that can persist after birth. Unfortunately, while developmental plasticity allows the embryo to survive these stressful conditions, such insult may lead to adult health problems and to long‐term effects on offspring that could be transmitted to subsequent generations. In this review, we describe how in mice, livestock, and humans, besides affecting the development of the embryo itself, ART stressors may also have significant repercussions on offspring health and physiology. Finally, we argue the case that better control of stressors during ART will help improve embryo quality and offspring health.