Miracle-dz/UniFAR-FTUs huggingface dataset:
Proteomic Profiling of Primary Human Acute Myeloid Leukemia Cells Does Not Reflect Their Constitutive Release of Soluble Mediators

Acute myeloid leukemia (AML) is a heterogeneous disease, and communication between leukemic cells and their neighboring leukemia-supporting normal cells is involved in leukemogenesis. The bone marrow cytokine network is therefore important, and the mediator release profile seems more important than single mediators. It is not known whether the characterization of primary AML cell proteomes reflects the heterogeneity of the broad and dynamic constitutive mediator release profile by these cells. To address this, we compared the intracellular levels of 41 proteins in 19 AML patients with the constitutive extracellular release during in vitro culture, including chemokines, growth factors, proteases, and protease regulators. The constitutive release of most mediators showed a wide variation (up to 2000-fold differences) between patients. Detectable intracellular levels were seen for 10 of 41 mediators, but for most of these 10 mediators we could not detect significant correlations between the constitutive release during in vitro culture and their intracellular levels. Intracellular protein levels in primary human AML cells do not reflect the dynamics, capacity, and variation between patients in constitutive mediator release profiles. Measurements of these profiles thus add complementary information to proteomic detection/quantification regarding the heterogeneity of the AML cell contributions to the bone marrow cytokine network.
Two-Factor-Based Public Data Protection Scheme in Smart Ocean Management

Nowadays, two-factor data security protection has become a research hotspot in smart ocean management. With the increasing popularity of smart ocean management, how to achieve the two-factor protection of public data resources in smart ocean management is a serious problem to be tackled. Furthermore, how to achieve both security and revocation is also a challenge for two-factor protection. In this paper, we propose a two-factor-based protection scheme with factor revocation in smart ocean management. The proposed scheme allows data owners (DOs) to send encrypted messages to users through a shipboard server (SS). The DOs are required to formulate access policy and perform attribute-based encryption on messages. In order to decrypt, the users need to possess two factors. The first factor is the user’s secret key. The second factor is security equipment, which is a sensor card in smart ocean system. The ciphertext can be decrypted if and only if the user gathers the key and the security equipment at the same time. What is more, once the security equipment is lost, the equipment can be revoked and a new one is redistributed to the users. The theoretical analysis and experiment results indeed indicate the security, efficiency, and practicality of our scheme.
Neural indicators of perceptual variability of pain across species

Significance While several features of brain activity can be used to predict the variability of painful percepts within a given individual, it is much more difficult to predict pain variability across individuals. Here, we used electrophysiology to sample brain activity of humans and rodents, and demonstrated that laser-induced gamma oscillations sampled by central electrodes predict pain sensitivity across individuals both reliably and selectively: reliably, because they consistently predict between-subject pain intensity in both humans and rodents; selectively, because they do not track the between-subject reported intensity of nonpainful but equally salient auditory, visual, and nonnociceptive somatosensory stimuli. This discovery indicates that variability in an individual’s pain sensitivity is, at least partly, explained by variability in the amplitude of gamma oscillations of that individual. Individuals exhibit considerable and unpredictable variability in painful percepts in response to the same nociceptive stimulus. Previous work has found neural responses that, while not necessarily responsible for the painful percepts themselves, can still correlate well with intensity of pain perception within a given individual. However, there is no reliable neural response reflecting the variability in pain perception across individuals. Here, we use an electrophysiological approach in humans and rodents to demonstrate that brain oscillations in the gamma band [gamma-band event-related synchronization (γ-ERS)] sampled by central electrodes reliably predict pain sensitivity across individuals. We observed a clear dissociation between the large number of neural measures that reflected subjective pain ratings at within-subject level but not across individuals, and γ-ERS, which reliably distinguished subjective ratings within the same individual but also coded pain sensitivity across different individuals. Importantly, the ability of γ-ERS to track pain sensitivity across individuals was selective because it did not track the between-subject reported intensity of nonpainful but equally salient auditory, visual, and nonnociceptive somatosensory stimuli. These results also demonstrate that graded neural activity related to within-subject variability should be minimized to accurately investigate the relationship between nociceptive-evoked neural activities and pain sensitivity across individuals.
Prevention of Malignant Transformation of Oral Leukoplakia and Oral Lichen Planus Using Laser: An Observational Study

Objectives: The present observational study was conducted with an aim to evaluate the efficacy of diode laser in management of homogenous oral leukoplakia (OL) and reticular oral lichen planus (OLP), so that these potentially malignant disorders can be limited and further malignant transformation can be prevented. Further the assessment of associated postoperative complications after laser therapy was also carried out. Study Design: Present study was carried out using diode laser 810nm on 60 subjects, of whom 30 subjects were of homogenous OL and 30 subjects were of reticular OLP aged between 20 – 60 years, the diagnosis of which was histopathologically confirmed. Results: Of the 60 subjects none complained of pain during and immediately after surgery with no bleeding at any stage of the procedure. By the end of 3rd day post operative most subjects reported no pain and swelling and very few subjects had negligible pain and swelling when evaluated. On subsequent follow-up of 1,2 and 4 week(s) none of the subject reported of pain, swelling or bleeding and it was noted that all the areas treated with laser had healed without scarring with no signs of recurrence. Fibrosis was seen in two male patients and one female patient treated for OL and OLP respectively. Conclusion: Patient compliance and contentment without any postoperative complications were observed to be of high degree in the present study. Thus diode laser can be considered as a best alternative to conventional surgical treatment modality in managing OL and OLP and preventing its further transformation.
Hand Related Disorders Following Axillary Dissection for Breast Cancer

Lymphedema is the most common complication of an axillary dissection with lymph node examination. A retrospective chart review and a detailed questionnaire were used to evaluate the prevalence of hand disorders in patients following breast surgery with an axillary dissection. The questionnaire was sent to 250 patients who had undergone an axillary dissection for breast can- cer. 143 patients returned the questionnaire and based upon the presence of hand numbness and tendinitic type pain patients were categorized as either symptomatic or asymptomatic. Forty-seven patients (33%) reported significant post-operative edema. Forty- five (31%) patients noted hand numbness or tingling, with diffi- culty in grasping objects, or weakness of the operated side. Fifteen (10%) of the patients had been given the diagnosis of carpal tunnel syndrome since surgery. Seventeen (12%) of patients noted click- ing, or sticking of their fingers or thumb and five (3%) had been given the diagnosis of a trigger digit. There was a significant difference between symptomatic and asymptomatic patients with regards to presence of lymphedema (p 4 0.001) and the amount of edema (p 4 0.03).
Psoriasis and the TNF/IL23/IL17 axis.

The excellent response of psoriasis to anti-TNF-α(TNF)/IL23/IL17A biologics implies a crucial role for the TNF/IL23/IL17 axis in developing psoriasis. In addition to the TNF/IL23/IL17 axis provided by immune cells, current evidence points to an important contribution of TNF, IL23 and IL17C produced from nonhematopoietic keratinocytes. Therefore, crosstalk between immune cells and keratinocytes forms a multilayered feed-forward loop to accelerate the TNF/IL23/IL17A axis. Many biologics have already been licensed or are under clinical trials. Given that the IL-17 signature is more upregulated in the skin than in synovium in psoriatic arthritis, anti-IL-23/IL-17 agents seem to be superior to anti-TNF-α remedies in the treatment of skin lesions. In this review, we summarize recent topics in psoriasis and the TNF/IL23/IL17 axis.
Globalization and identity development among emerging adults from Ladakh.

OBJECTIVES Around the world, the transformative power of globalization has increased intercultural connectivity, initiated sociocultural change, and broadened the possibilities for diverse life trajectories. These developments have increased the number of individuals within non-Western contexts who experience emerging adulthood as a developmental life stage between adolescence and adulthood. Central to this life stage is identity development, which has become increasingly challenging during times of rapid sociocultural change. The present study investigated the interplay among cultural orientation, personal identity development, and psychological well-being during the processes of globalization-based acculturation in the North Indian Himalayan region of Ladakh. METHOD A sample of 487 Ladakhi college students studying in Leh (72.9% female) and Delhi (48.0% female), ranging in age from 17 to 28 years, completed self-report questionnaires about cultural orientation, personal identity development, and psychological well-being. RESULTS Local Ladakhi cultural endorsement was not directly associated with psychological well-being. Furthermore, both personal identity exploration and commitment appeared to mediate the relationship between cultural endorsement and psychological well-being. Endorsement of traditional Ladakhi and Indian cultural streams was related to identity commitment and indirectly to psychological well-being. Endorsement of the global Western cultural stream was associated with identity exploration and indirectly with psychological well-being. CONCLUSION Results illustrate the key role of personal identity among indigenous non-Western emerging adults exposed to cultural globalization. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
Fluorescence modulation of excited state intramolecular proton transfer (ESIPT) probe 3-formyl-4-hydroxy benzoic acid encapsulated in the protein binding domain of serum albumins: a combined spectroscopic and molecular docking study

The interaction and binding behavior of excited state intramolecular proton transfer (ESIPT) probe 3-formyl-4-hydroxy benzoic acid (FHBA) with bovine and human serum albumins (BSA and HSA, respectively) was monitored through a series of biophysical techniques including steady state and time-resolved fluorescence as well as molecular docking calculation. The fluorescence emission of FHBA can be tuned from selective excited state species due to its spontaneous association with protein binding sites in a cooperative manner. On the other hand, Stern- Volmer analysis of the tryptophan fluorescence quenching data in presence of FHBA reveals the ligand- protein association constant (Ka) and other thermodynamic parameters like enthalpy (? H) and entropy (? S) change corresponding to the ligand binding process. The results show that FHBA binds into the sub-domain IIA of the proteins in 1:1 stoichiometry with an apparent binding constant value in the range of ∼104 M1. In both the cases, FHBA binds spontaneously with the proteins through entropy driven hydrophobic mechanism, although the interaction with BSA is relatively stronger in comparison with HSA. The fluorescence titration results are corroborated nicely with the supplement experiments like circular dichroism (CD), isothermal titration calorimetry (ITC) and also with the results from molecular docking calculation.
Regulation of cardiac myocyte cohesion and gap junctions via desmosomal adhesion

Mutations in desmosomal proteins can induce arrhythmogenic cardiomyopathy with life‐threatening arrhythmia. Previous data demonstrated adrenergic signalling to be important to regulate desmosomal cohesion in cardiac myocytes. Here, we investigated how signalling pathways including adrenergic signalling, PKC and SERCA regulate desmosomal adhesion and how this controls gap junctions (GJs) in cardiac myocytes.
Health-related quality of life and anxiety in the PAN-CAN lung cancer screening cohort

Objectives The impact of lung cancer screening with low-dose chest CT (LDCT) on participants’ anxiety levels and health-related quality of life (HRQoL) is an important consideration in the implementation of such programmes. We aimed to describe changes in anxiety and HRQoL in a high-risk Canadian cohort undergoing LDCT lung cancer screening. Methods 2537 subjects who had 2% or greater lung cancer risk over 6 years using a risk prediction tool were recruited from eight centres across Canada in the Pan-Canadian Early Detection of Lung Cancer Study (2008–2010). We compared HRQoL and anxiety levels before and after screening of 1237 participants with LDCT (excluding a subset of 1300 participants who also underwent autofluorescence bronchoscopy screening), as well as after investigations performed because of a positive screening examination. The 12-item short-form Physical and Mental Component Scales (SF-12), EQ-5D-3L scores and State Trait Anxiety Inventory-State anxiety were used at each assessment. Results Overall, there were no clinically significant differences in HRQoL outcomes between baseline and each of the survey time points following initial screening. No mean change in anxiety in the overall cohort was noted following baseline LDCT, but more participants had clinically significant increase in anxiety versus decrease after baseline screening (increase >minimal clinically important difference (MCID) (n=180) vs decrease >MCID (n=50), p<0.001). This finding persisted but to a lesser degree at the 12 month time point (increase >MCID (n=146) vs decrease >MCID (n=87), p<0.001). Conclusions CT screening for lung cancer has no major overall impact on HRQoL among participants, although a minority of participants (number-needed-to-harm=7 after baseline screening and 18 at 1 year) demonstrated clinically significant increased anxiety levels. Trialregistration number NCT00751660; Results.
Phage Therapy in the Postantibiotic Era

Antibiotic resistance is arguably the biggest current threat to global health. An increasing number of infections are becoming harder or almost impossible to treat, carrying high morbidity, mortality, and financial cost. SUMMARY Antibiotic resistance is arguably the biggest current threat to global health. An increasing number of infections are becoming harder or almost impossible to treat, carrying high morbidity, mortality, and financial cost. The therapeutic use of bacteriophages, viruses that infect and kill bacteria, is well suited to be part of the multidimensional strategies to combat antibiotic resistance. Although phage therapy was first implemented almost a century ago, it was brought to a standstill after the successful introduction of antibiotics. Now, with the rise of antibiotic resistance, phage therapy is experiencing a well-deserved rebirth. Among the admittedly vast literature recently published on this topic, this review aims to provide a forward-looking perspective on phage therapy and its role in modern society. We cover the key points of the antibiotic resistance crisis and then explain the biological and evolutionary principles that support the use of phages, their interaction with the immune system, and a comparison with antibiotic therapy. By going through up-to-date reports and, whenever possible, human clinical trials, we examine the versatility of phage therapy. We discuss conventional approaches as well as novel strategies, including the use of phage-antibiotic combinations, phage-derived enzymes, exploitation of phage resistance mechanisms, and phage bioengineering. Finally, we discuss the benefits of phage therapy beyond the clinical perspective, including opportunities for scientific outreach and effective education, interdisciplinary collaboration, cultural and economic growth, and even innovative use of social media, making the case that phage therapy is more than just an alternative to antibiotics.
Leprosy survey among rural communities and wild armadillos from Amazonas state, Northern Brazil

There is evidence that in southern US, leprosy is a zoonosis infecting wild Dasypus novemcinctus armadillos but the extent of this finding is unknown. This ecological study investigated leprosy in rural communities and in wild armadillos from the Brazilian Amazon. The study area was the Mamiá Lake of Coari municipality, Amazonas State, Northern region, a hyper endemic leprosy area where residents live on subsistence farming, fishing and armadillo hunting and its meat intake are frequent. The leprosy survey was conducted in sixteen communities by a visiting team of specialists. Local partakers provided wild armadillos to investigate M. leprae infection. Volunteers had complete dermato-neurological examination by a dermatologist with expertise in leprosy diagnosis, suspect skin lesions were biopsied for histopathology (Hematoxylin-eosin/HE, Fite-Faraco/FF staining); slit skin smears were collected. Armadillos’ tissue fragments (skins, spleens, livers, lymph nodes, adrenal glands, others) were prepared for histopathology (HE/FF) and for M. leprae repetitive element-RLEP-qPCR. Among 176 volunteers, six new indeterminate leprosy cases were identified (incidence = 3.4%). Suspect skin sections and slit skin smears were negative for bacilli. Twelve wild D. novemcinctus were investigated (48 specimens/96 slides) and histopathological features of M. leprae infection were not found, except for one skin presenting unspecific inflammatory infiltrate suggestive of indeterminate leprosy. Possible traumatic neuroma, granuloma with epithelioid and Langhans cells, foreign-body granuloma were also identified. Granulomatous/non-granulomatous dermatitides were periodic-acid-Schiff/PAS negative for fungus. M. leprae-RLEP-qPCR was negative in all armadillos’ tissues; no bacillus was found in histopathology. Our survey in rural communities confirmed the high endemicity for leprosy while one armadillo was compatible with paucibacillary M. leprae infection. At least in the highly endemic rural area of Coari, in the Brazilian Amazon region where infectious sources from untreated multibacillary leprosy are abundant, M. leprae infected armadillos may not represent a major source of infection nor a significant public health concern.
Dietary l-Tryptophan Supplementation Enhances the Intestinal Mucosal Barrier Function in Weaned Piglets: Implication of Tryptophan-Metabolizing Microbiota

l-Tryptophan (Trp) is known to play an important role in the health of the large intestine. However, a role of dietary Trp in the small-intestinal mucosal barrier and microbiota remains poorly understood. The present study was conducted with weaned piglets to address this issue. Postweaning piglets were fed for 4 weeks a corn- and soybean meal-based diet supplemented with 0 (Control), 0.1, 0.2, or 0.4% Trp. The small-intestinal microbiota and serum amino acids were analyzed by bacterial 16S rRNA gene-based high-throughput sequencing methods and high-performance liquid chromatography, respectively. The mRNA levels for genes involved in host defense and the abundances of tight-junction proteins in jejunum and duodenum were measured by real time-PCR and Western blot techniques, respectively. The concentrations of Trp in the serum of Trp-supplemented piglets increased in a dose-dependent manner. Compared with the control group, dietary supplementation with 0.2–0.4% Trp reduced the abundances of Clostridium sensu stricto and Streptococcus in the jejunum, increased the abundances of Lactobacillus and Clostridium XI (two species of bacteria that can metabolize Trp) in the jejunum, and augmented the concentrations of secretory immunoglobulin A (sIgA) as well as mRNA levels for porcine β-defensins 2 and 3 in jejunal tissues. Moreover, dietary Trp supplementation activated the mammalian target of rapamycin signaling and increased the abundances of tight-junction proteins (zonula occludens (ZO)-1, ZO-3, and claudin-1) in jejunum and duodenum. We suggested that Trp-metabolizing bacteria in the small intestine of weaned pigs primarily mediated the beneficial effects of dietary Trp on its mucosal integrity, health, and function.
Estilo de Aprendizaje Visual: una estrategia educativa para el desarrollo de la memoria a largo plazo

This article presents the results of the investigation based in the visual learning style as a strategy for long-term memory which it aims to create a better process in the knowledge acquisition to help students. In this way, the visual style will make easier to the student retain information for a long-term. In adittion, this article contains a theorical description about the learning styles and memory. In this investigation has been elaborated two measuring instruments that was applied to 40 students belonging to seventh year in the Teaching Unit Francisco Flor in three phases: an initial screening, an observation datasheet with the application of encourage activities, and a final test. Following, the analysis and interpretation of datum was realized and the verification of the hypothesis too. The results of the initial test and the final test were weighted; with it can calculate the Z-Test. It shows that exist a development of the memory in the long-term by educational strategies.