Miracle-dz/UniFAR-FTUs huggingface dataset:
Preclinical Disability

Accomplishing a compression of morbidity will require prevention of disability, a significant component of illness in older adults. To do this, better understanding is needed of the natural history of functional loss prior to disability. The authors hypothesize that there is an identifiable stage of preclinical disability that will predict future disability and identify a focus for prevention. It is proposed, based on observations in the Johns Hopkins Functional Status Laboratory, that preclinical disability is characterized by persons who perceive no difficulty with performance of a task and yet are found to have either (a) general diminution in activities requiring related abilities, or (b) changes in the performance of specific tasks. Both models are due to impairments. The authors suggest that nondisabled persons use compensatory strategies that minimize functional restrictions resulting from impairment; this may keep functional decline at a preclinical level. These hypotheses, if verified in prospective studies, may provide a basis for early detection of those at risk of disability and suggest effective preventive measures.
Investigating the efficacy of pamidronate, a chemical inhibitor of farnesyl pyrophosphate synthase, in the inhibition of influenza virus infection in vitro and in vivo.

Influenza A virus has caused significant pandemics in the past decades, including the H1N1‑2009 pandemic. Viperin is an interferon‑inducible protein that acts as a broad‑spectrum antiviral protein via the inhibition of farnesyl pyrophosphate synthase (FPPS). To mimic this activity of viperin, the present study investigated the effectiveness of a commercially available FPPS inhibitor (pamidronate) as an inhibitor of influenza virus infection in vitro and in vivo. HeLaM cells were treated with pamidronate to determine its effect on the replication of influenza virus A/H1N1/WSN/1933. C57BL/6 mice were also subjected to intratracheal pamidronate treatment regimes prior to and following lethal influenza challenge. Treatment with the FPPS inhibitor in vitro resulted in a considerable reduction in the viral titer of ~1 log and diminished lipid raft formation without cellular toxicity, thus mimicking the antiviral effect of viperin. However, pamidronate lacked efficacy in vivo and was associated with increased pulmonary damage, most likely due to the complexity of drug‑host interactions in the infected mice. Further studies are warranted on pamidronate treatment in other infectious diseases that are more susceptible to FPPS inhibition.
Sequential Recommendation with User Memory Networks

User preferences are usually dynamic in real-world recommender systems, and a user»s historical behavior records may not be equally important when predicting his/her future interests. Existing recommendation algorithms -- including both shallow and deep approaches -- usually embed a user»s historical records into a single latent vector/representation, which may have lost the per item- or feature-level correlations between a user»s historical records and future interests. In this paper, we aim to express, store, and manipulate users» historical records in a more explicit, dynamic, and effective manner. To do so, we introduce the memory mechanism to recommender systems. Specifically, we design a memory-augmented neural network (MANN) integrated with the insights of collaborative filtering for recommendation. By leveraging the external memory matrix in MANN, we store and update users» historical records explicitly, which enhances the expressiveness of the model. We further adapt our framework to both item- and feature-level versions, and design the corresponding memory reading/writing operations according to the nature of personalized recommendation scenarios. Compared with state-of-the-art methods that consider users» sequential behavior for recommendation, e.g., sequential recommenders with recurrent neural networks (RNN) or Markov chains, our method achieves significantly and consistently better performance on four real-world datasets. Moreover, experimental analyses show that our method is able to extract the intuitive patterns of how users» future actions are affected by previous behaviors.
Factors determining family support and quality of life of elderly population

Background: Aging is an unavoidable and irreversible change as a result of demographic transition in all societies. Family support for the elderly people has become a very important issue in examining the overall well-being of the elderly people. Family support is playing a key role in determining the quality of life (QOL) of the aged people. Objective: The main aim of the research was to find the factors determining the QOL and family support of elderly people. Material and Methods: An exploratory descriptive design was followed to obtain error-free results. A total of 213 elderly people were consecutively recruited from a randomly selected setting. Results: The elderly men reported a better quality of life. Elderly people who had received a formal edu-cation also reported a good quality of life. Those who were financially independent had a healthier QOL. Medically healthy elderly people had enhanced QOL. Those who performed their daily activities inde-pendently also had superior QOL. Elderly people who got support from their family members had an en-hanced QOL. Those who reported having no major problems in their life also had a superior QOL. The educated elderly people had perceived better family support. Medically healthy aged people perceived better family support. Conclusion: This study found that there are various factors such as gender, education, financial inde-pendence, and family support determined the QOL of the aged. Similarly, education, wealth, and family support were the predictors for family support of the elderly people.
Overcoming CD8+ T-Cell Exhaustion in Viral Hepatitis: Lessons from the Mouse Model and Clinical Perspectives

About 500 million people all over the world are chronically infected with hepatitis B virus (HBV) or hepatitis C virus (HCV) and are thus at high risk of developing liver fibrosis, cirrhosis or hepatocellular carcinoma. While in adults about 90% of acutely HBV-infected patients clear the virus, only 30% of acute HCV infections clear spontaneously. Several mechanisms contribute to the failure in viral clearance. The main factors responsible for the chronification of HBV and HCV infection are, on the one hand, viral escape mutations leading to lack of recognition by antiviral immune cells and, on the other hand, loss of antiviral effector functions of virus-specific CD8+ T cells, called T-cell exhaustion. This review focuses on the latter highlighting current knowledge about the heterogeneity of exhausted CD8+ T cells and the potential for re-invigoration of exhausted T-cell populations during chronic viral hepatitis. Although direct-acting antivirals successfully clear chronic HCV infection, there is still the need for a prophylactic vaccine to prevent primary infection. Moreover, a therapeutic strategy eliminating HBV infection still does not exist. A better understanding of T-cell exhaustion and the potential for functional recovery will help to develop new immunotherapeutic approaches for chronic viral hepatitis.
How different are students and their learning styles

Introduction: Students, like anybody else differ from each other. As students they differ in their preferred mode of learning, i.e. their preferred modes in gathering, organizing and thinking about information. A recent classification proposed by Neil Fleming and associates state that students learning styles can be divided into Visual/graphic, Aural, Read/write and Kinesthetic types, VARK. Aim: The aim of the recent study is to investigate learning styles among dental students in two different dental colleges of India. Method: The VARK-questionnaire contains 15 multiple-choice- questions with four possibilities to select an answer. Each possibility represents one of the four modes of perception. But, one can select more than one answer to each question, which is necessary for the identification of poly modal modes of perception and learning. This is also a psychometric problem when trying to state a measure of the reliability of the questionnaire. The VARK-questionnaire was distributed among 200 students and was collected back. This sample size represents 100% response rate from the students in the class and is markedly above the level required to make conclusions about student preferences for receiving and processing information. The students spent about 10 minutes in an ordinary lesson to fill in the questionnaire. Students register number and name were used in the study and there was no blinding practiced. Study Design: Questionnaire based clinical study Results: The responses from the students in our University where classified into multi-modal (VARK), tri-modal (VRK, VAK, VAR, ARK), bi-modal (VR, VA, VK, RK) and uni-modal (V, A, R.K) categories. Results showed that subjects had a higher preference for multimodal learning. Conclusion: We conclude that students in our set up prefer multimodal and more of Kinesthetic of learning. To meet their needs, a variation in teaching, learning and examination must be implemented. If not, these students with a high kinesthetic preference for perception and learning may be at the losing end.
In vivo confocal intrinsic optical signal identification of localized retinal dysfunction.

PURPOSE The purposes of this study were to investigate the physiological mechanism of stimulus-evoked fast intrinsic optical signals (IOSs) recorded in dynamic confocal imaging of the retina, and to demonstrate the feasibility of in vivo confocal IOS mapping of localized retinal dysfunctions. METHODS A rapid line-scan confocal ophthalmoscope was constructed to achieve in vivo confocal IOS imaging of frog (Rana pipiens) retinas at cellular resolution. In order to investigate the physiological mechanism of confocal IOS, comparative IOS and electroretinography (ERG) measurements were made using normal frog eyes activated by variable-intensity stimuli. A dynamic spatiotemporal filtering algorithm was developed to reject the contamination of hemodynamic changes on fast IOS recording. Laser-injured frog eyes were employed to test the potential of confocal IOS mapping of localized retinal dysfunctions. RESULTS Comparative IOS and ERG experiments revealed a close correlation between the confocal IOS and retinal ERG, particularly the ERG a-wave, which has been widely used to evaluate photoreceptor function. IOS imaging of laser-injured frog eyes indicated that the confocal IOS could unambiguously detect localized (30 μm) functional lesions in the retina before a morphological abnormality is detectable. CONCLUSIONS The confocal IOS predominantly results from retinal photoreceptors, and can be used to map localized photoreceptor lesion in laser-injured frog eyes. We anticipate that confocal IOS imaging can provide applications in early detection of age-related macular degeneration, retinitis pigmentosa, and other retinal diseases that can cause pathological changes in the photoreceptors.
Expert Statement: Pneumothorax Associated with Endoscopic Valve Therapy for Emphysema - Potential Mechanisms, Treatment Algorithm, and Case Examples

The use of endoscopically placed unidirectional valves for the treatment of emphysema is increasing. With better patient selection, there is also an increased likelihood of complications associated with the procedure, such as postprocedural pneumothorax. There is, however, little evidence of pneumothorax management in patients with severe COPD and emphysema. This report describes an expert recommendation that has been developed to outline pneumothorax management after valve placement to inform physicians and patients of the risk-benefit profile and to assist them in decision making. Skilled and aggressive pneumothorax management is necessary in this patient population, and by following these recommendations traumatic scenarios, prolonged drainage, extended hospitalizations, and/or surgery might be avoided in many cases.
Pulmonary , gastrointestinal and urogenital pharmacology Dopamine treatment attenuates acute kidney injury in a rat model of deep hypothermia and rewarming – The role of renal H 2 S-producing enzymes

Hypothermia and rewarming produces organ injury through the production of reactive oxygen species. We previously found that dopamine prevents hypothermia and rewarming-induced apoptosis in cultured cells through increased expression of the H2S-producing enzyme cystathionine β-Synthase (CBS). Here, we investigate whether dopamine protects the kidney in deep body cooling and explore the role of H2S-producing enzymes in an in vivo rat model of deep hypothermia and rewarming. In anesthetized Wistar rats, body temperature was decreased to 15 °C for 3 h, followed by rewarming for 1 h. Rats (nZ5 per group) were treated throughout the procedure with vehicle or dopamine infusion, and in the presence or absence of a non-specific inhibitor of H2S-producing enzymes, amino-oxyacetic acid (AOAA). Kidney damage and renal expression of three H2S-producing enzymes (CBS, CSE and 3-MST) was quantified and serum H2S level measured. Hypothermia and rewarming induced renal damage, evidenced by increased serum creatinine, renal reactive oxygen species production, KIM-1 expression and influx of immune cells, which was accompanied by substantially lowered renal expression of CBS, CSE, and 3-MST and lowered serum H2S levels. Infusion of dopamine fully attenuated renal damage and maintained expression of H2S-producing enzymes, while normalizing serum H2S. AOAA further decreased the expression of H2S-producing enzymes and serum H2S level, and aggravated renal damage. Hence, dopamine preserves renal integrity during deep hypothermia and rewarming likely by maintaining the expression of renal H2S-producing enzymes and serum H2S. & 2015 Elsevier B.V. All rights reserved.
Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-{alpha}-mediated inflammation and insulin resistance in primary human adipocytes.

BACKGROUND Quercetin and trans-resveratrol (trans-RSV) are plant polyphenols reported to reduce inflammation or insulin resistance associated with obesity. Recently, we showed that grape powder extract, which contains quercetin and trans-RSV, attenuates markers of inflammation in human adipocytes and macrophages and insulin resistance in human adipocytes. However, we do not know how quercetin and trans-RSV individually affected these outcomes. OBJECTIVE The aim of this study was to examine the extent to which quercetin and trans-RSV prevented inflammation or insulin resistance in primary cultures of human adipocytes treated with tumor necrosis factor-α (TNF-α)-an inflammatory cytokine elevated in the plasma and adipose tissue of obese, diabetic individuals. DESIGN Cultures of human adipocytes were pretreated with quercetin and trans-RSV followed by treatment with TNF-α. Subsequently, gene and protein markers of inflammation and insulin resistance were measured. RESULTS Quercetin, and to a lesser extent trans-RSV, attenuated the TNF-α-induced expression of inflammatory genes such as interleukin (IL)-6, IL-1β, IL-8, and monocyte chemoattractant protein-1 (MCP-1) and the secretion of IL-6, IL-8, and MCP-1. Quercetin attenuated TNF-α-mediated phosphorylation of extracellular signal-related kinase and c-Jun-NH₂ terminal kinase, whereas trans-RSV attenuated only c-Jun-NH₂ terminal kinase phosphorylation. Quercetin and trans-RSV attenuated TNF-α-mediated phosphorylation of c-Jun and degradation of inhibitory κB protein. Quercetin, but not trans-RSV, decreased TNF-α-induced nuclear factor-κB transcriptional activity. Quercetin and trans-RSV attenuated the TNF-α-mediated suppression of peroxisome proliferator-activated receptor γ (PPARγ) and PPARγ target genes and of PPARγ protein concentrations and transcriptional activity. Quercetin prevented the TNF-α-mediated serine phosphorylation of insulin receptor substrate-1 and protein tyrosine phosphatase-1B gene expression and the suppression of insulin-stimulated glucose uptake, whereas trans-RSV prevented only the TNF-α-mediated serine phosphorylation of insulin receptor substrate-1. CONCLUSION These data suggest that quercetin is equally or more effective than trans-RSV in attenuating TNF-α-mediated inflammation and insulin resistance in primary human adipocytes.
Usefulness of PHQ-9 in primary care to determine meaningful symptoms of low mood: a qualitative study.

BACKGROUND Self-administered questionnaires, such as the Patient Health Questionnaire (PHQ-9), are regularly used in clinical practice to guide prescribing or to measure recovery and response to treatment. There are concerns that patients are not all interpreting the questionnaire items in the same way. Cognitive interviewing is a research technique that identifies 'interpretative measurement error' (IME). IME is distinct from traditional components of measurement error, such as not reading the question as worded, or recording answers inaccurately. AIM To use cognitive interviewing techniques to explore patterns in answer mapping and comprehension of the PHQ-9 questionnaire to ascertain whether the measure captures meaningful symptoms of low mood. DESIGN AND SETTING Qualitative study using cognitive interviewing techniques and card sorting in six GP practices in Bristol. METHOD The study recruited 18 participants at the point of entry to a longitudinal primary care depression cohort study, PANDA (the indications for Prescribing ANtiDepressants that will leAd to a clinical benefit). Participants were interviewed 2, 4, and 6 weeks after their baseline visit. Cognitive interviews were digitally recorded. Analysis used the digital audio file, rather than verbatim transcripts, as it retained important features needed for analyses. RESULTS Cognitive interviewing revealed that items on the PHQ-9 are interpreted in a range of ways, that patients often cannot 'fit' their experience into the response options, and therefore often feel the questionnaire is misrepresenting their experience of meaningful symptoms of low mood. CONCLUSION The PHQ-9 may be missing the presence and/or intensity of certain symptoms that are meaningful to patients. Clinicians should adopt caution when using it.
GC/MS Analysis of Citrus aurantium L. Hydrolate and its Comparison with the Commercial Samples GC/MS Analysis of Citrus aurantium L. Hydrolate and its Comparison with the Commercial Samples

The chemical composition of the Hydrolate of Citrus aurantium L. (Rutaceae) flowers (neroli) grown in Iran, and two commercial hydrolates were analyzed by GC/MS. Thirty compounds (90.3%) were identified in the Hydrolate by the use of laboratory apparatus, thirty-eight compounds (83.4%) in the Hydrolate from the traditional method and fifteen compounds (98.3%) in the industrially produced sample. The major compounds within the Hydrolate obtained in the laboratory were geraniol (26.6%), a-terpineol (20.7%), linalool (15.4%) and benzene acetaldehyde (5.5%). Linalool (44.1%), methyl anthranilate (11.8%) and cis-linalool oxide (6.1%) were found in high percentages in the Hydrolate from the obtained traditional sample. 1,8-Cineol (15.9%), linalool (13.8%) and a-terpineol (6.6%) were more than other constituents in the industrially obtained hydrolate.
Osteoporosis-Pseudoglioma Syndrome: Three Novel Mutations in the LRP5 Gene and Response to Bisphosphonate Treatment

Background/Aims: Osteoporosis-pseudoglioma (OPPG) syndrome is a rare disorder characterized by congenital or infancy-onset visual loss and severe juvenile osteoporosis. OPPG is caused by homozygous mutations in the low-density lipoprotein receptor-related protein 5 (LRP5) gene. We present three novel homozygous LRP5 mutations found in 3 unrelated Turkish children with consanguineous parents, along with clinical phenotypes and response to treatment with bisphosphonates (bisP). Methods/Results: The LRP5 gene was analyzed by direct sequencing after PCR amplification. Mutation screening for LRP5 revealed homozygous nonsense R1002X mutation in the first patient and homozygous missense mutations V336M and G507S in the second and third patient, respectively. The parents were heterozygous for these mutations. The patients’ eye symptoms began during the first months of life but the OPPG diagnoses were made based on skeletal deformities and osteopenia after 4 years of age. The patients’ bone mineral density Z scores were very low and consistent with osteopenia. All patients were treated with bisP for 3.5–7 years. Conclusion: We report three novel LRP5 mutations in 3 Turkish patients with OPPG. We show that the response of bisP therapy has improved the lumbar spinal bone mineral density Z scores and the patients’ quality of life as the bone pains decreased.
SPECT/CT in the Management of Osteochondral Lesions of the Talus

Background: The treatment of osteochondral lesion of the talus (OLT) is mainly based upon the stage of the disease so accurate imaging is crucial. SPECT/CT combines bone scan with high-resolution CT and can provide functional-anatomical images in a single stage. The purpose of this study was to assess the value of SPECT/CT in the management of OLT. Materials and Methods: From 2004 to 2009, 22 patients with OLT were identified that had both SPECT/CT and MRI of the foot and ankle. All charts were reviewed to ascertain the reason for ordering the SPECT/CT and the additional information obtained. AOFAS outcome scores were calculated at the time of followup. Results: Twelve patients underwent ankle arthroscopy for debridement or drilling of the osteochondral lesion. The mean AOFAS score in these 12 patients was 83.6. SPECT/CT helped preoperative planning by identifying the exact location of the active lesion, especially in multifocal disease or revision surgeries while showing the depth of the active lesion. Ten patients had conservative management due to minimal or no activity over the lesion on SPECT/CT images. The mean AOFAS score in these ten patients was 78.8 which was comparable to the operative group. Conclusion: We believe SPECT/CT was able to provide additional diagnostic value by demonstrating a co-existing pathology as a potential cause of pain and in preoperative planning by showing the depth of activity and the precise location of the active segment in multiple lesions. Level of Evidence: III, Retrospective Case Control Study
Evaluating current practice in the provision of written information to stroke patients and their carers

This study aimed to determine current practice in the provision of written information to 57 stroke patients and their carers. It also explored their informational needs while in hospital and 6months later and examined the suitability of the written materials received, comparing readability levels to participants' general reading ability. While in hospital, 22.8% of patients and 41.7% of carers received written information, yet 91.2% of patients and 100% of carers wanted information. More than half of the participants wanted information on preventing strokes, causes and risk factors of stroke, recovery, what a stroke is, stroke-related medications and sources of further information. At 6months after stroke, 75.5% of patients and all carers wanted further information. The mean SMOG readability level of the written materials received was equivalent to a grade 11 level of education, compared to the patients' mean reading ability, which was equivalent to a 7th–8th grade reading level. The authors conclude tha.