Miracle-dz/UniFAR-FTUs huggingface dataset:
Examination of Food Chain-Derived Listeria monocytogenes Strains of Different Serotypes Reveals Considerable Diversity in inlA Genotypes, Mutability, and Adaptation to Cold Temperatures

ABSTRACT Listeria monocytogenes strains belonging to serotypes 1/2a and 4b are frequently linked to listeriosis. While inlA mutations leading to premature stop codons (PMSCs) and attenuated virulence are common in 1/2a, they are rare in serotype 4b. We observed PMSCs in 35% of L. monocytogenes isolates (n = 54) recovered from the British Columbia food supply, including serotypes 1/2a (30%), 1/2c (100%), and 3a (100%), and a 3-codon deletion (amino acid positions 738 to 740) seen in 57% of 4b isolates from fish-processing facilities. Caco-2 invasion assays showed that two isolates with the deletion were significantly more invasive than EGD-SmR (P < 0.0001) and were either as (FF19-1) or more (FE13-1) invasive than a clinical control strain (08-5578) (P = 0.006). To examine whether serotype 1/2a was more likely to acquire mutations than other serotypes, strains were plated on agar with rifampin, revealing 4b isolates to be significantly more mutable than 1/2a, 1/2c, and 3a serotypes (P = 0.0002). We also examined the ability of 33 strains to adapt to cold temperature following a downshift from 37ï¿½C to 4ï¿½C. Overall, three distinct cold-adapting groups (CAG) were observed: 46% were fast (<70 h), 39% were intermediate (70 to 200 h), and 15% were slow (>200 h) adaptors. Intermediate CAG strains (70%) more frequently possessed inlA PMSCs than did fast (20%) and slow (10%) CAGs; in contrast, 87% of fast adaptors lacked inlA PMSCs. In conclusion, we report food chain-derived 1/2a and 4b serotypes with a 3-codon deletion possessing invasive behavior and the novel association of inlA genotypes encoding a full-length InlA with fast cold-adaptation phenotypes.
Beyond the day-ahead market – effects of revenue maximisation of the marketing of renewables on electricity markets

The proportion of renewable energy has rapidly increased in many countries during the last few years. Due to the specific characteristics of variable renewables, this development influences the price on electricity markets as well as flexibility requirements in the electricity system. New developments regarding market liberalisation and support schemes for renewables encourage the active participation of renewables in the electricity market. This paper analyses the effects of such a participation of renewables in different electricity market segments. In particular, we investigate how the active marketing of renewables on the day-ahead, intra-day, balancing and futures markets dampen their effects on markets and systems. Using German data for 2013, we determine the effect of direct marketing on average market price levels and price volatility, the possible contribution of renewables to balancing, the profitability of flexible generation from biomass, and the additional revenues that renewables can generate from participating in different markets. Price effects of shifting renewables between markets, and limits in intra-day market liquidity are included in the assessment.
Climatological Estimation of Environmental Uncertainty Over the Middle Atlantic Bight Shelf and Slope

Historical hydrographic data are used to determine the spatial and seasonal patterns of uncertainty in thermohaline and sound-speed fields in a well-sampled region, the continental shelf and slope in the Middle Atlantic Bight (MAB). Several different historical databases are combined to produce two-dimensional (2-D) plan view and cross-shelf fields of temperature, salinity, and sound speed in two separate regions, the New England shelf and the shelf off Delaware and Maryland. In addition, spatial maps of the sound-speed fields reveal that the maximum variance of the sound speed occurs at the edge of the continental shelf, in the vicinity of the shelfbreak front. The standard deviation of the sound speed was largest during the spring and summer, with magnitudes as large as 14 m/s in a narrow band coinciding with the mean position of the shelfbreak front. During spring the peak in variance was located near the surface outcrop of the front, but during summer the maximum variance was centered at a depth of 30 m, immediately beneath the seasonal thermocline. Comparisons with both synoptic measurements from the Shelfbreak PRIMER experiment as well as moored time series from the Nantucket Shoals Flux Experiment confirm that the shelfbreak front is a "hotspot" of uncertainty (maximum variance), and that the vertical structure of the peak variance is dependent on the presence or absence of the seasonal thermocline
GCMB, a second human homolog of the fly glide/gcm gene

Glial cells play fundamental roles in neurogenesis. In addition, defective or abnormal gliogenesis is associated with severe diseases. To understand the molecular basis of such diseases, it is crucial to identify the genes promoting normal gliogenesis. Here we identify GCMB, which encodes a human protein homologous to the fly glial promoting factor glial cell deficient/glial cell missing (glide/gcm).
Do existing psychologic scales measure the nonspecific benefit associated with CAM treatment?

OBJECTIVE To determine whether existing psychologic well-being scales are sensitive to change after complementary and alternative medicine (CAM) treatment, and whether changes in those scales of well-being correlate with symptom change. This may limit the need for the development of new CAM-specific outcomes. DESIGN A study investigating change on several outcome measures over a 4-month period during CAM treatment. Patients attending the Centre for Complementary and Integrated Medicine (CCIM, Southampton, UK) for their first appointment were recruited and completed their baseline forms (T1) at the first consultation. Three (3) further sets of questionnaires (T2, T3 and T4) were mailed to them with a stamped addressed envelope at monthly intervals and were returned to CCIM. PATIENTS AND LOCATIONS: People visiting the CCIM were treated by 1 of 3 practitioners with an individualized combination of homeopathy, dietary advice, and nutritional supplements for treatment of their chronic benign illness. OUTCOME The previously validated outcome measures were as follows: (1) symptoms (Measure Yourself Medical Outcome Profile: MYMOP); (2) mood (Positive and Negative Affect Scale; PANAS); and (3) Brief Assessment of Sense of Coherence (BASOC). RESULTS Forty-five (45) patients were recruited and 40 completed the study; MYMOP (p=0.001), PANAS negative (p=0.025), and BASOC (p=0.019) all showed similar patterns of significant improvement over time; PANAS positive showed a nonsignificant trend for improvement (p=0.074). Change on one scale was correlated with change on other scales. CONCLUSIONS Existing psychology scales of well-being are sensitive to change after CAM treatment and consistent with symptom improvement. Existing measures of positive affect provide an alternative to the negative, symptom-driven approach of conventional medicine.
Isoflurane Impairs Immature Astroglia Development In Vitro: The Role of Actin Cytoskeleton

General anesthetics, either alone or in combination, can be detrimental to the developing mammalian brain and induce extensive apoptotic degeneration of immature neurons when they are administered at the peak of synaptogenesis. Because neuron development and normal functions depend on the integrity of astroglia, we sought to determine whether general anesthesia also causes disturbances in the early development of astroglia. Using isoflurane, an inhaled anesthetic that is highly toxic to immature neurons, we studied primary astroglia cultures, focusing on very early development (Day-In-Vitro 4 treatment). Exposure to 3% isoflurane for 24 hours delayed morphological differentiation and impaired the growth of immature astrocytes. The timing of delayed astroglia maturation and growth coincided with a major disturbance in actin cytoskeleton sculpting that was manifest as impaired actin stress fiber formation and cytoskeletal organization and downregulation of the focal adhesion protein, paxillin. Isoflurane-induced actin cytoskeletal changes were accompanied by a significant decrease in protein levels of the endogenous GTPase RhoA that regulates the phosphorylation of myosin light chain protein, suggesting that isoflurane-induced impairment in glial growth and morphological development is, in part, mediated by the RhoA/myosin light chain protein signaling pathway.
Body image and depressive symptoms in person suffering from psoriasis.

OBJECTIVES The purpose of the research study was specifying the relationship between severity of psoriasis and body image and self-reported depressive symptoms, taking into account the differences between the sexes. METHODS The research study involved 54 psoriasis patients, including 30 men and 24 women aged from 19 to 82. The level of depressive symptoms was assessed using the Beck Depression Inventory, and body image - using the Multidimensional Body-Self Relations Questionnaire. The disease severity was objectively assessed using the Psoriasis Area and Severity Index. RESULTS The female psoriasis patients were more critical of their appearance than the men (p< 0.01), and at the same time they were more appearance-oriented (p< 0.05). The men rated their fitness level (p< 0.01) and care for good physical condition (p< 0.01) higher than the women. The women showed less satisfaction with their body parts than the men (p< 0.01) and more fear of obesity (p< 0.05) and overweight (p< 0.05). No statistically significant differences were found between men and women with regard to general evaluation of body image and self-reported depressive symptoms. Moreover, it was established that in the case of both women and men there was a correlation between lowered mood and psoriasis severity (R = 0.416), as well as body image (R = - 0.282). In relation to individual scales, there was a statistically significant relationship between self-reported depressive symptoms and appearance evaluation (R = - 0.519), health evaluation (R = - 0.585), satisfaction with body parts (R = - 0.462), as well as appearance orientation (R = 0.425). CONCLUSIONS Distortion of body image is correlated with self-reported depressive symptoms in psoriasis patients.
Repetitive transcranial magnetic stimulation of the motor cortex ameliorates spasticity in multiple sclerosis

Objective: To investigate whether repetitive transcranial magnetic stimulation (rTMS) can modify spasticity. Methods: We used high-frequency (5 Hz) and low-frequency (1 Hz) rTMS protocols in 19 remitting patients with relapsing–remitting multiple sclerosis and lower limb spasticity. Results: A single session of 1 Hz rTMS over the leg primary motor cortex increased H/M amplitude ratio of the soleus H reflex, a reliable neurophysiologic measure of stretch reflex. Five hertz rTMS decreased H/M amplitude ratio of the soleus H reflex and increased corticospinal excitability. Single sessions did not induce any effect on spasticity. A significant improvement of lower limb spasticity was observed when rTMS applications were repeated during a 2-week period. Clinical improvement was long-lasting (at least 7 days after the end of treatment) when the patients underwent 5 Hz rTMS treatment during a 2-week protocol. No effect was obtained after a 2-week sham stimulation. Conclusions: Repetitive transcranial magnetic stimulation may improve spasticity in multiple sclerosis.
Long-Term Surgical Results of Pubovaginal Sling Procedure Using Polypropylene Mesh in the Treatment of Stress Urinary Incontinence

Introduction: Surgery for stress urinary incontinence (SUI) in women might cure incontinence symptom butdevelop new problems. This study assessed the long-term outcome of pubovaginal sling procedure using polypropylene mesh in patients with SUI. Materials and Methods: A total of 108 patients with various types of SUI received pubovaginal sling procedure using polypropylene mesh. A surgical technique that did not create bladder outlet obstruction after operation was used in all the patients. Video urodynamic study was performed at baseline, 2 weeks and 3 months after operation. The long-term surgical results and patients’ satisfaction were assessed. Results: The age of patients ranged from 33 to 94 years (mean 62.6 ± 12.0 years). The follow-up period ranged from 24 to 72 months (median 46 months). Among the 108 patients, a successful result was noted in 92 (85.2%), including a continence rate of 65.7% and mild SUI in 19.4% of patients. Treatment failure was noted in 16 patients (14.8%), including 13 (12%) with urge incontinence and 3 (2.8%) with severe SUI that was cured by a second sling. There were 104 patients (96.3%) who could void volitionally with little residual urine, whereas 4 (3.7%) needed transvaginal urethrolysis. Urodynamic study revealed no significant changes in the mean values of parameters at 3 months after operation. However, 22 (20%) patients had an increase in voiding pressure by 50% at 3 months postoperatively. The reported satisfactory rate was 89.8%. Conclusions: Pubovaginal sling procedure using polypropylene mesh had a high success rate in all types of SUI. The continence rate was suboptimal, indicating that the absence of bladder outlet obstruction might result in inadequate urethral compression after pubovaginal sling procedure.
Pilot assessment of the antineoplastic drug contamination levels in British Columbian hospitals pre- and post-cleaning

Objective. We undertook a pilot study involving six British Columbian hospital pharmacies to determine if antineoplastic drug contamination of surfaces exists and whether residual drugs remain on these surfaces despite being cleaned. Methods. At each site, the pharmacy technician responsible for preparing the antineoplastic drugs was observed to determine which surfaces were contacted and to ascertain the frequency of contact. Surfaces observed to be most frequently contacted were subsequently wiped after drug preparation pre- and post-clean. The wipe samples were then analyzed by liquid chromatography tandem mass spectrometry to determine the amount of contamination. Cyclophosphamide (CP) and methotrexate (MTX) were used as representative markers to reflect overall antineoplastic drug contamination levels. Results. Fourteen of the 23 surfaces sampled pre-clean (61%) were contaminated with either MTX or CP. The pre-clean wipe samples had a geometric mean concentration of 0.0135 ng/cm2 for MTX (range <Limit of Detection {LOD} to 12.45 ng/cm2) and 0.114 ng/cm2 for CP (range <LOD to 8.53 ng/cm2). Post-clean contamination levels were generally lower than its pre-clean equivalent; the concentration difference post- vs. pre-clean was statistically significant for CP only. However, some samples appeared to have higher post-clean contamination levels. Conclusions. The results suggest that drug contamination is common in hospital pharmacies we sampled and that current cleaning practices in British Columbia may not be effective in removing residual drug from the surfaces. A more extensive study is recommended to confirm these results as well as a review of cleaning protocols to ensure their effectiveness in reducing contamination levels.
Contribution of functional voltage‐gated Na+ channel expression to cell behaviors involved in the metastatic cascade in rat prostate cancer: II. Secretory membrane activity

The secretory membrane activities of two rat prostate cancer cell lines of markedly different metastatic potential, and corresponding electrophysiological characteristics, were studied in a comparative approach. In particular, voltage‐gated Na+ channels (VGSCs) were expressed in the strongly metastatic MAT‐LyLu but not in the closely related, but weakly metastatic, AT‐2 cells. Uptake and release of the non‐cytotoxic marker horseradish peroxidase (HRP) were used as indices of general endocytotic and exocytotic membrane activity, respectively. The amount of tracer present in a given experimental condition was quantified by light microscopic digital imaging. The uptake of HRP was an active process, abolished completely by incubating the cells at low temperature (5°C) and suppressed by disrupting the cytoskeleton. Interestingly, the extent of HRP uptake into the strongly metastatic MAT‐LyLu cells was almost twice that into the weakly metastatic AT‐2 cells. Vesicular uptake of HRP occurred in a fast followed by a slow phase; these appeared to correspond to cytoplasmic and perinuclear pools, respectively. Importantly, the overall quantitative difference in the uptake disappeared in the presence of 1 μM tetrodotoxin which significantly reduced the uptake of HRP into the MAT‐LyLu cells. There was no effect on the AT‐2 cells, consistent with functional VGSC expression occurring selectively in the former. A similar effect was observed in Na+‐free medium. The uptake was partially dependent upon extracellular Ca2+ but was not affected by raising the extracellular K+ concentration. We suggest that functional VGSC expression could potentiate prostate cancer cells' metastatic ability by enhancing their secretory membrane activity. © 2003 Wiley‐Liss, Inc.
Inhibition of NF- (cid:1) B Sensitizes A431 Cells to Epidermal Growth Factor-induced Apoptosis, whereas Its Activation by Ectopic Expression of RelA Confers Resistance*

Epidermal growth factor (EGF) is a well known mitogen, but it paradoxically induces apoptosis in cells that overexpress its receptor. We demonstrate for the first time that the EGF-induced apoptosis is accelerated if NF- (cid:1) B is inactivated. To inactivate NF- (cid:1) B, human epidermoid carcinoma cells (A431) that overexpress EGF receptor were stably transfected with an I (cid:1) B- (cid:2) double mutant construct. Under the NF- (cid:1) B-inactivated condition, A431 cells were more sensitive to EGF with decreased cell viability and increased externalization of phosphatidylserine on the cell surface, DNA fragmentation, and activation of caspases (3 and 8 but not 9), typical features of apoptosis. These results were further supported by the potentiation of the growth inhibitory effects of EGF by chemical inhibitors of NF- (cid:1) B (curcumin and sodium salicylate) and the protective role of RelA evidenced by the resistance of A431-RelA cells (stably transfected with RelA) to EGF-induced apoptosis. EGF treatment or ectopic expression of RelA in A431 cells induced DNA binding activity of NF- (cid:1) B (p50 and RelA) and the expression of c-IAP1, a (1:3000),
Translation of the Dizziness Handicap Inventory into Chinese, Validation of it, and Evaluation of the Quality of Life of Patients with Chronic Dizziness

The aims of this study were 1) to translate the Dizziness Handicap Inventory (DHI) into Chinese and to validate it and 2) to use the Chinese DHI and the Chinese (Hong Kong) Short Form-36 Health Survey (Chinese [HK] SF-36) to evaluate the quality of life (QoL) of patients with chronic dizziness. Seventy-one patients with 6 months of dizziness due to vestibular dysfunction were evaluated initially, 7 days later (n = 49), and after 7 months of medical treatment (n = 17). The Chinese DHI has been shown to retain good test-retest reliability (intraclass correlation coefficient range, 0.64 to 0.87) and internal consistency (Cronbach α coefficient > 0.7) as compared to the original DHI and has a moderate responsiveness (0.54). We find the Chinese DHI to be a valid tool for evaluation of QoL of Chinese patients with dizziness. Low mean scores on the Chinese DHI and Chinese (HK) SF-36 signify that chronic dizziness has a considerable impact on the QoL of these patients.
Identification of Dehydrin-Like Proteins Responsive to Chilling in Floral Buds of Blueberry (Vaccinium, section Cyanococcus)

The level of three major polypeptides of 65, 60, and 14 kD increased in response to chilling unit accumulation in floral buds of a woody perennial, blueberry (Vaccinium, section Cyanococcus). The level of the polypeptides increased most dramatically within 300 h of chilling and decreased to the prechilling level with the initiation of budbreak. Cold-hardiness levels were assessed for dormant buds of Vaccinium corymbosum and Vaccinium ashei after different chilling treatments until the resumption of growth. These levels coincided with the level of the chilling-responsive polypeptides. Like some other previously described cold-induced proteins in annual plants, the level of the chilling-induced polypeptides also increased in leaves in response to cold treatment; the chilling-induced polypeptides were heat stable, resisting aggregation after incubation at 95[deg]C for 15 min. By fractionating bud proteins first by isoelectric point (pl) and then by molecular mass, the pl values of the 65- and 60-kD polypeptides were found to be 7.5 to 8.0 and the pl value of the 14-kD polypeptide was judged to be 8.5. Purification of the 65- and 60-kD polypeptides, followed by digestion with endoproteinase Lys-C and sequencing of selected fragments, revealed similarities in amino acid composition between the 65- and 60-kD polypeptides and dehydrins. Indeed, antiserum to the lysine-rich consensus sequence EKKGIMDKIKEKLPG of dehydrin proteins cross-reacted to all three of the major chilling-responsive polypeptides of blueberry, identifying these as dehydrins or dehydrin-like proteins.
Humoral immune responses and CD27+ B cells in children with DiGeorge syndrome (22q11.2 deletion syndrome)

The spectrum of T‐cell abnormalities in 22q11.2 syndrome is quite broad, ranging from profound and life threatening to non‐existent defects. Humoral abnormalities have been described in some of these patients, although no data are currently available on their phenotypical and functional B cell subsets. The purpose of this study was to investigate humoral immune function in a cohort of 13 children with DiGeorge syndrome by immunophenotyping B and by analysing their functionality in vivo. Humoral immunity was assessed by serum immunoglobulin evaluation, IgG subclasses determination, and testing of specific antibody titers to recall antigens. B cells were analyzed by flow cytometry and the relevant percentage of membrane surface expression of CD27, IgM, IgD was evaluated. In our cohort, one of 13 children (7.7%) had a complete IgA deficiency, four of 13 (30.7%) had minor immunoglobulin abnormalities, and five (38%) had an impaired production of specific antibodies. Five of 13 children (38%) had recurrent infections. Interestingly, peripheral CD27+ B cells were reduced in our patients as compared with age‐matched healthy controls, and this decrement was statistically significant for IgM+ IgD+ CD27+ B cells. Immunoglobulin abnormalities were associated with the occurrence of recurrent infections. We conclude that a significant proportion of patients with DiGeorge syndrome have defective humoral immunity, which may represent an additional pathogenic mechanism underlying the increased susceptibility to infections. Whether the decreased CD27+ B‐cell subset might be one of the defects that contribute to impaired humoral immunity, and to susceptibility to infection remains to be elucidated.