Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparative Analysis of the Frequency and Distribution of Stem and Progenitor Cells in the Adult Mouse Brain

The neurosphere assay can detect and expand neural stem cells (NSCs) and progenitor cells, but it cannot discriminate between these two populations. Given two assays have purported to overcome this shortfall, we performed a comparative analysis of the distribution and frequency of NSCs and progenitor cells detected in 400 μm coronal segments along the ventricular neuraxis of the adult mouse brain using the neurosphere assay, the neural colony forming cell assay (N‐CFCA), and label‐retaining cell (LRC) approach. We observed a large variation in the number of progenitor/stem cells detected in serial sections along the neuraxis, with the number of neurosphere‐forming cells detected in individual 400 μm sections varying from a minimum of eight to a maximum of 891 depending upon the rostral‐caudal coordinate assayed. Moreover, the greatest variability occurred in the rostral portion of the lateral ventricles, thereby explaining the large variation in neurosphere frequency previously reported. Whereas the overall number of neurospheres (3730 ± 276) or colonies (4275 ± 124) we detected along the neuraxis did not differ significantly, LRC numbers were significantly reduced (1186 ± 188, 7 month chase) in comparison to both total colonies and neurospheres. Moreover, approximately two orders of magnitude fewer NSC‐derived colonies (50 ± 10) were detected using the N‐CFCA as compared to LRCs. Given only 5% of the LRCs are cycling (BrdU+/Ki‐67+) or competent to divide (BrdU+/Mcm‐2+), and proliferate upon transfer to culture, it is unclear whether this technique selectively detects endogenous NSCs. Overall, caution should be taken with the interpretation and employment of all these techniques.
Impact of European Union Generalized System of Preferences scheme on fruit and vegetable exports from East Africa: A preference margin approach

We evaluate the impact of the Generalized System of Preferences (GSP) scheme on horticultural exports from Kenya, Tanzania and Uganda to the European Union (EU). The preference margin, computed as the difference between trade-weighted Most Favoured Nation’s rate and the ad valorem equivalents(, is used as a proxy for the GSP scheme. The zero-inflated Poisson estimator is used to control for overdispersion and excess zero trade flows, while time-invariant effects control for heterogeneity. The findings suggest that the EU-GSP scheme promotes bean exports from the three East African states as well as pepper from Uganda. Conversely, the results suggest that the scheme seems not to enhance export of asparagus from Kenya, vegetables from Tanzania and bananas from Uganda to the EU.
Real and Monetary Policy Convergence: EMU Crisis to the CFA Zone

A major lesson of the EMU crisis is that serious disequilibria result from regional monetary arrangements not designed to be robust to a variety of shocks. The purpose of this paper is to assess these disequilibria within the CEMAC, UEMOA and CFA zones. In the assessments, monetary policy targets inflation and financial dynamics of depth, efficiency, activity and size while real sector policy targets economic performance in terms of GDP growth. We also provide the speed of convergence and time required to achieve a 100% convergence. But for financial intermediary size within the CFA zone, findings for the most part support only unconditional convergence. There is no form of convergence within the CEMAC zone. The broad insignificance of conditional convergence results have substantial policy implications. Monetary and real policies which are often homogenous for member states are thwarted by heterogeneous structural and institutional characteristics which give rise to different levels and patterns of financial intermediary development. Therefore member states should work towards harmonizing cross-country differences in structural and institutional characteristics that hamper the effectiveness of monetary policies.
The Role of Interference Control in Working Memory: A Study with Children at Risk of Adhd

The study aimed to test whether the impairment in a working memory task observed in children at risk of ADHD was due to a lack of control of interfering information being processed whilst carrying out the memory task. Two groups of children at risk of ADHD with or without a learning disability (reading impairment) were compared to a control group in a working memory task. Activation of irrelevant items was tested with a lexical decision task presented immediately after the final recall in about half of the trials. Results showed a poor working memory performance in children at risk of ADHD and reading disability associated with a larger activation of irrelevant information than that of control children. Results indicated that the to-be-excluded and interfering items are still highly accessible to working memory in children that fail the working memory task. The examination of working memory and interference control of children at risk of ADHD with a learning disability revealed a counterintuitive picture of children with poor working memory showing better recall/activation of processed information. This picture is consistent with a view of working memory related to an efficient inhibitory control that influences cognitive functioning.
Physiological and Pharmacological Roles of Vascular Nucleoside Transporters

Abstract Adenosine modulates various vascular functions such as vasodilatation and anti-inflammation. The local concentration of adenosine in the vicinity of adenosine receptors is fine tuned by 2 classes of nucleoside transporters: equilibrative nucleoside transporters (ENTs) and concentrative nucleoside transporters (CNTs). In vascular smooth muscle cells, 95% of adenosine transport is mediated by ENT-1 and the rest by ENT-2. In endothelial cells, 60%, 10%, and 30% of adenosine transport are mediated by ENT-1, ENT-2, and CNT-2, respectively. In vitro studies show that glucose per se increases the expression level of ENT-1 via mitogen-activating protein kinase–dependent pathways. Similar results have been demonstrated in diabetic animal models. Hypertension is associated with the increased expression of CNT-2. It has been speculated that the increase in the activities of ENT-1 and CNT-2 may reduce the availability of adenosine to adenosine receptors, thereby weakening the vascular functions of adenosine. This may explain why patients with diabetes and hypertension suffer greater morbidity from ischemia and atherosclerosis. No oral hypoglycemic agents can inhibit ENTs, but an exception is troglitazone (a thiazolidinedione that has been withdrawn from the market). ENTs are also sensitive to dihydropyridine-type calcium-channel blockers, particularly nimodipine, which can inhibit ENT-1 in the nanomolar range. Those calcium-channel blockers are noncompetitive inhibitors of ENTs, probably working through the reversible interactions with allosteric sites. The nonsteroidal anti-inflammatory drug sulindac sulfide is a competitive inhibitor of ENT-1. In addition to their original pharmacological actions, it is believed that the drugs mentioned above may regulate vascular functions through potentiation of the effects of adenosine.
An extended two‐point dixon algorithm for calculating separate water, fat, and B0 images

A new algorithm is presented that provides separate water, fat, and B0 images utilizing the in‐phase and opposed‐phase acquisitions of the two‐point Dixon (2PD) method. The accuracy of the extended method (E2PD) compares favorably with the three‐point Dixon (3PD) method, and the acquisition requires 2/3 the 3PD scan time. Slightly increased mismapping may occur in pixels containing an admixture of water and fat due to reduced SNR in the B0 field map compared with the 3PD method.
Effect of Surgical Caseload on Revision Rate Following Total and Unicompartmental Knee Replacement.

BACKGROUND High-volume surgeons attain the best results following unicompartmental knee replacement (UKR), but the exact relationship between caseload and outcome is not clear. It is not known whether this effect is due to patient selection or surgical skill nor whether a similar effect is seen in total knee replacement (TKR). The aim of this study was to quantify the effect of surgical caseload on survival of both TKR and UKR. METHODS This study was based on 459,280 patient records (422,149 TKRs and 37,131 UKRs) from the National Joint Registry for England and Wales. The caseload-outcome relationship was characterized graphically and quantified using regression techniques. Patient selection was compared among high, medium, and low-volume surgeons. Prosthetic survival was compared between UKRs (performed by high, medium, and low-volume surgeons) and matched TKRs. RESULTS Caseload affected survival of TKR and, more strongly, of UKR. The revision rate following UKR dropped steeply until the volume reached ten cases per year, plateauing at thirty cases. For surgeons performing fewer than ten UKRs per year, the mean eight-year rate of survival of the UKRs was 87.9% (95% confidence interval [CI] = 86.9% to 88.8%) compared with 92.4% (95% CI = 90.9% to 93.6%) for those who performed thirty UKRs or more per year. Analysis of the TKRs showed a linear decrease in revision rate as caseload increased (hazard ratio [HR] for revision = 0.99 [95% CI = 0.98 to 0.99] for every five-case increase in caseload). Surgeons who performed a lower volume of UKRs tended to operate on younger and healthier patients and were more likely to perform revisions to treat loosening and pain. After matching of patients who had undergone UKR with those who had undergone TKR, the surgeons who performed a high volume of UKRs were found to have an eight-year revision/revision rate similar to that seen after TKR (HR for revision or reoperation = 1.10 [95% CI = 0.99 to 1.22] favoring TKR). CONCLUSIONS This study confirmed the importance of surgical caseload in determining the survival of UKR and, to a lesser extent, TKR. The reasons for this effect are complex and not fully explained by variables recorded in the National Joint Registry; however, the patient selection and revision threshold of lower-volume surgeons may be a factor. Examination of matched patients in this study demonstrated that high-volume surgeons can achieve revision/reoperation rates similar to those observed following TKR.
ADAPTIVE RADIATION ALONG GENETIC LINES OF LEAST RESISTANCE

Are measurements of quantitative genetic variation useful for predicting long‐term adaptive evolution? To answer this question, I focus on gmax, the multivariate direction of greatest additive genetic variance within populations. Original data on threespine sticklebacks, together with published genetic measurements from other vertebrates, show that morphological differentiation between species has been biased in the direction of gmax for at least four million years, despite evidence that natural selection is the cause of differentiation. This bias toward the direction of evolution tends to decay with time. Rate of morphological divergence between species is inversely proportional to θ, the angle between the direction of divergence and the direction of greatest genetic variation. The direction of greatest phenotypic variance is not identical with gmax, but for these data is nearly as successful at predicting the direction of species divergence. I interpret the findings to mean that genetic variances and covariances constrain adaptive change in quantitative traits for reasonably long spans of time. An alternative hypothesis, however, cannot be ruled out: that morphological differentiation is biased in the direction gmax because divergence and gmax are both shaped by the same natural selection pressures. Either way, the results reveal that adaptive differentiation occurs principally along “genetic lines of least resistance. ”
Corporate Social Responsibility Disclosure, Political Connection and Tax Aggressiveness: Evidence from China’s Capital Markets

Corporate social responsibility disclosure, political connection and tax aggressiveness have become the focus of the media. By using samples of China’s listed firms from 2008 to 2014, this study examines the relationship among corporate social responsibility disclosure, political connection and tax aggressiveness. The results show that corporate social responsibility disclosure significantly strengthens the possibility of tax aggressiveness; firms with no or less close political connections can make use of the disclosure of corporate social responsibility to do tax aggressiveness. Furthermore, the change of political connection can significantly weaken the positive association between corporate social responsibility disclosure and tax aggressiveness. However, some data are collected by hands and that may cause some deviations. These findings help governments, managements and investors evaluate firm’s behavior and make decisions.
Inhibition of p38 MAPK Attenuates Ionizing Radiation-Induced Hematopoietic Cell Senescence and Residual Bone Marrow Injury

Exposure to a moderate or high total-body dose of radiation induces not only acute bone marrow suppression but also residual (or long-term) bone marrow injury. The induction of residual bone marrow injury is primarily attributed to the induction of hematopoietic cell senescence by ionizing radiation. However, the mechanisms underlying radiation-induced hematopoietic cell senescence are not known and thus were investigated in the present study. Using a well-established long-term bone marrow cell culture system, we found that radiation induced hematopoietic cell senescence at least in part via activation of p38 mitogen-activated protein kinase (p38). This suggestion is supported by the finding that exposure to radiation selectively activated p38 in bone marrow hematopoietic cells. The activation was associated with a significant reduction in hematopoietic cell clonogenic function, an increased expression of p16INK4a (p16), and an elevated senescence-associated β-galactosidase (SA-β-gal) activity. All these changes were attenuated by p38 inhibition with a specific p38 inhibitor, SB203580 (SB). Selective activation of p38 was also observed in bone marrow hematopoietic stem cells (HSCs) after mice were exposed to a sublethal total-body dose (6.5 Gy) of radiation. Treatment of the irradiated mice with SB after total-body irradiation (TBI) increased the frequencies of HSCs and hematopoietic progenitor cells (HPCs) in their bone marrow and the clonogenic functions of the irradiated HSCs and HPCs. These findings suggest that activation of p38 plays a role in mediating radiation-induced hematopoietic cell senescence and residual bone marrow suppression.
Comprehensive Cyclic Voltammetry Characterization of AIROF Microelectrodes

Cyclic voltammetry (CV) at slow scan rate is usually performed to characterize the electrochemical behavior of microelectrodes. Early in vitro study on over 200 activated iridium oxide film (AIROF) microelectrodes showed variability of their electrochemical behavior. In this paper, we reported CV measurements under 3 different scan rates for 96 AIROF microelectrodes. We traced their behavior during 7 stages of fabrication and processing starting from prior to activation, and ending with post-implantation in the experimental animal. The results show that even at the early stage of electrode activation, variations between near identical electrodes existed
The association between body mass index and musculoskeletal foot disorders: a systematic review

The primary aim of this systematic review was to investigate the relationship between body mass index (BMI) and foot disorders. The secondary aim was to investigate whether weight loss is effective for reducing foot pain. Five electronic databases (Ovid MEDLINE, Ovid EMBASE, Ovid AMED, CINAHL and The Cochrane Library) and reference lists from relevant papers were searched in April 2011. Twenty‐five papers that reported on the association between BMI and musculoskeletal foot disorders met our inclusion criteria and were reviewed. The evidence indicates: (i) a strong association between increased BMI and non‐specific foot pain; and (ii) a strong association between increased BMI and chronic plantar heel pain in a non‐athletic population. The evidence is inconclusive regarding the relationship between BMI and the following specific disorders of the foot; hallux valgus, tendonitis, osteoarthritis and flat foot. With respect to our second aim, there were only two prospective cohort studies that reported a reduction in foot symptoms following weight loss surgery. In summary, increased BMI is strongly associated with non‐specific foot pain in the general population and chronic plantar heel pain in a non‐athletic population. However, there is currently limited evidence to support weight loss to reduce foot pain.
The Per regulon of enteropathogenic Escherichia coli : identification of a regulatory cascade and a novel transcriptional activator, the locus of enterocyte effacement (LEE)‐encoded regulator (Ler)

Enteropathogenic Escherichia coli (EPEC) is the prototype organism of a group of pathogenic Gram‐negative bacteria that cause attaching and effacing (AE) intestinal lesions. All EPEC genes necessary for the AE phenotype are encoded within a 35.6 kb pathogenicity island termed the locus of enterocyte effacement (LEE). The LEE encodes 41 predicted open reading frames (ORFs), including components of a type III secretion apparatus and secreted molecules involved in the disruption of the host cell cytoskeleton. To initiate our studies on regulation of genes within the LEE, we determined the genetic organization of the LEE, defining transcriptional units and mapping transcriptional start points. We found that components of the type III secretion system are transcribed from three polycistronic operons designated LEE1, LEE2 and LEE3. The secreted Esp molecules are part of a fourth polycistronic operon designated LEE4. Using reporter gene fusion assays, we found that the previously described plasmid‐encoded regulator (Per) activated operons LEE1, LEE2 and LEE3, and modestly increased the expression of LEE4 in EPEC. Using single‐copy lacZ fusions in K‐12‐derived strains, we determined that Per only directly activated the LEE1::lacZ fusion, and did not directly activate the other operons. Orf1 of the LEE1 operon activated the expression of single‐copy LEE2::lacZ and LEE3::lacZ fusions in trans and modestly increased the expression of LEE4::lacZ in K‐12 strains. Orf1 was therefore designated Ler, for LEE‐encoded regulator. Thus, the four polycistronic operons of the LEE that encode type III secretion components and secreted molecules are now included in the Per regulon, where Ler participates in this novel regulatory cascade in EPEC.
Throw Me a Lifeline: A Comparison of Port Cities with Antithetical Adaptation Strategies to Sea-Level Rise

THROW ME A LIFELINE: A COMPARISON OF PORT CITIES WITH ANTITHETICAL ADAPTATION STRATEGIES TO SEA-LEVEL RISE Claudia Marie Risner Old Dominion University, 2018 Director: Dr. Regina Karp Sea-level rise (SLR) is a manifestation of climate change that is particularly hazardous to port cities that must remain on the waterfront to function, yet are increasingly battered and flooded by encroaching storms, and sinking into the rising saltwater. Despite sharing a common high level of risk, port cities are choosing antithetical adaptation strategies that range from hard-engineered structural flood protection, to behavioral modifications, to innovative softengineered measures, to doing nothing at all. Why is this? Are transnational city networks, such as C40 Cities, a lifeline to drowning cities? Do differences in governance structure, financial capacity, risk tolerance to the hazard, or the influence of special interest groups matter? These factors and the interplay of civil, public, and corporate actors in the context of changing environmental conditions are examined in this cross-disciplinary qualitative study to understand their effects on adaptation decision-making processes over time. Four at-risk global port cities—Venice, Rotterdam, Guangzhou, and Miami—were selected for comparison based on their antithetical adaptation strategies of retreating, climate proofing, innovating, and denying. The Panarchy model of nested four-stage adaptive renewal cycles frames the ongoing and cross-scalar interaction of stakeholders and special interest groups at the city, national, transnational, and international levels. This methodology enables the identification of patterns, power distributions, and path dependencies that contribute to appropriate or maladaptive adaptation. As is characteristic of complex adaptive systems, this study finds that decisions cannot be correlated with a single factor. For those cities that display key characteristics of resilience, SLR is a catalyst for proactive and appropriate adaptation. For others, socio-economic and socio-political factors trump environmental factors in deciding whether, when, and how a city decreased its risk to SLR hazard.
Digital Radiographic Evaluation of the Level of Alveolar Bone Crest in External Hexagon Implants Submitted to 2 Types of Implant Abutments Under Immediate Loading

PurposeThe aim of this study was to evaluate by means of digital radiography the behavior of the alveolar bone crest in external hexagon implants following the use of 2 different types of abutments, one for conventional cemented prosthesis and one for modified cemented prosthesis. MethodsTen external hexagon implants (platform 4.1) were placed in 5 patients. Initial instrumentation was carried out to obtain primary stability of the temporary prostheses under immediate loading. Each patient received both abutments for conventional and modified cemented prosthesis. Standardized digital periapical radiographies were performed at times T0 (immediately after implant placement) and T1 (4 months after implant placement). A straight line was initially established from the implant platform to the distal and mesial peri-implantar marginal bone tissue (immediately in contact with the implant) and measured by digital radiography, using Sidexis version 2.3 (Sirona Dental Systems GmbH, Bensheim, Germany) software. The data were submitted to paired-samples t-test analysis. ResultsThere was no significant difference between the conventional and modified cemented prosthesis. In both cases, t-test results were within the null hypothesis level. ConclusionThe abutment for the modified cemented prosthesis resulted in no significant radiographic difference of alveolar bone crest height, when compared with the conventional cemented prostheses.