Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibitory effect of the coffee diterpene kahweol on carrageenan‐induced inflammation in rats

Previous studies reported that kahweol, a coffee‐specific diterpene, inhibits cyclooxygenase‐2 (COX‐2) and inducible nitric oxide synthase (iNOS) expression in cultured lipopolysaccharide‐activated macrophages. The aim of this study was to confirm the anti‐inflammatory effects of kahweol by examining its effect on the inflammatory response induced by carrageenan in a rat using an acute air pouch inflammation model. Kahweol significantly reduced the levels of the inflammatory process markers in the air pouch, such as the volume of exudates, the amount of protein and the number of leukocytes and neutrophils. The levels of nitrite, TNF‐a and prostaglandin E2 (PGE2) were also markedly lower in the air pouch of the kahweol‐treated animals than in the controls. Immunoblot analysis showed that kahweol reduced the COX‐2 and iNOS expression level in the exudate cells. The histological examination showed that there was a lower inflammatory response in the pouch tissues from the kahweol‐treated animals. In addition, kahweol significantly reduced the paw edema induced by carrageenan and also markedly reduced the level of PGE2 production in the inflamed paw. These results suggest that kahweol has significant anti‐inflammatory effects in vivo, which might be due to the inhibition of iNOS and COX‐2 expression in the inflammatory sites.
The stability of the demand for money function in Islamic and non-Islamic monetary policy regimes

This study, using quarterly data from Egypt and Iran, extends the literature on demand for money by examining the stability of money demand functions in two different monetary policy regimes, an Islamic banking system and a conventional banking system. A stable demand for money enables central banks accurately to predict the demand for money and hence attain a price stability objective through the adjustment of the money supply. This paper adopts a restructured form of Friedman's (1956) model, which considers real demand for money as an extension to the theory of demand for durable goods. The study estimates the long-run demand for money functions in Iran, which represents an Islamic banking system, and Egypt, which represents a conventional banking system. The study then examines empirically the stability of the demand for money function under two different financial systems. The study finds that the demand for money function is stable under the Islamic banking system and unstable under the interest-based banking system.
Expression of cyclins and cyclin dependent kinases in human benign and malignant melanocytic lesions*

Aims—The regulation of cell proliferation is a key event in normal development, pathophysiological responses to injury, and tumorigenesis. The orderly progression of cells through the cell cycle depends on a finely tuned balance between the concentrations of activated cyclins and cyclin dependent kinases. This study was undertaken to compare the expression of cell cycle regulators in benign and malignant melanocytic lesions during tumour progression. Methods—Immunohistochemistry was used to analyse 49 primary cutaneous malignant melanomas, 18 metastatic melanomas, and 12 histologically confirmed naevus cell naevi for their expression of cyclins (A, B1, D1, D2, D3, and E) and cyclin dependent kinases (CDK1, CDK2, and CDK4). Results—Cyclin E and CDK2 had the highest expression patterns in human cutaneous melanomas and metastases and correlated positively with histological type and tumour stage. Cyclins B1, D2, and D3 had significantly increased expression in metastases, but normal or even decreased expression in primary melanomas. However, cyclins A and D1, and CDK1 and CDK4 were expressed very weakly in situ with no significant differences between naevi, melanomas, or metastases, and there was no correlation with histopathological staging. The specificity of recognition by the antibodies used was confirmed by western blotting on a panel of seven human melanoma cell lines. Cyclins A, B, and E were expressed by all seven, whereas cyclin D1 was detectable in six of seven and CDK2 and cdc2 were present in five of seven lines analysed. Conclusions—Taken together, this study demonstrated a significant increase of cyclin E and CDK2 expression during tumour progression in malignant melanomas.
Outage State Optimal Load Allocation by Automatic Sectionalizing Switches Operation in Distribution Systems

This paper presents a systematic and practical algorithm for load transfer by automatic sectionalizing switch operation in distribution systems at a fault occurrence subject to the transformer-capacity constraints and the line-capacity constraints on condition that all the section loads are estimated. The algorithm is developed by introducing the concept of subset sum problem and network structure. Computer experience with a real system indicates that the algorithm proposed here is valid and effective for practical operations.
Factors Associated with Treatment Failure in Vertebral Osteomyelitis Requiring Spinal Instrumentation

ABSTRACT Patients with vertebral osteomyelitis may require instrumentation for spinal stabilization. Determining the optimal duration and type of antimicrobial therapy for these patients is challenging. The aim of this study was to examine risk factors for treatment failure, in particular antimicrobial duration, in a cohort of patients requiring spinal instrumentation for vertebral osteomyelitis. We conducted a retrospective cohort study of all patients with vertebral osteomyelitis who had spinal instrumentation between January 2002 and January 2012 at the University of Maryland Medical Center. The primary outcome measure was treatment failure >4 weeks postoperatively. We identified 131 patients with vertebral osteomyelitis requiring spinal instrumentation, 94 of whom had >4 weeks of follow-up and were included in the primary analysis. Treatment failure occurred in 22 of the 94 patients (23%) at a median of 4 months after surgery. Among patients who failed therapy, 20 of 22 failed within 1 year of surgery. Cervical and thoracic infection sites and the presence of negative cultures were associated with fewer treatment failures. Addition of rifampin and the use of chronic suppressive antimicrobials did not affect treatment failure rate. Twenty-three percent of patients with spinal instrumentation for vertebral osteomyelitis experienced treatment failure. Treatment failure almost always occurred within the first year of spinal instrumentation.
Validation of remote mapping of cochlear implants

Recipients of cochlear implants need to attend regular mapping sessions to adjust their speech processors. We developed a PC-based system for remote mapping, which employed voice, video and text communication. Eleven implant recipients had their implant electrodes programmed (mapped) both by conventional methods and remotely. Speech tests, conducted by a family member or research assistant, were used to test the outcome of the programming during the remote consultation. The recipient and audiologist were surveyed regarding aspects of the teleconsultation. There were no significant differences between conventionally and remotely programmed electrode settings. The speech test results were perfect in most cases. The average time to complete the conventional mapping session was 37 min and the average time for the remote mapping session was 42 min (P = 0.034). Recipients and the audiologist reported favourably on most aspects of the consultations. Lack of synchronisation between voice and video was the most common problem, which disrupted communication. The assistant was important to overcome this problem. All but one participant indicated they would be willing to use tele-mapping in the future. Remote programming of cochlear implants can be conducted reliably with relatively simple equipment, potentially in the homes of remotely located patients assisted by a family member.
Osteoarthritis prevention

Purpose of reviewWe discuss recent published epidemiologic data regarding risk factors for incident and progressive knee osteoarthritis and related knee pain to identify targets for primary and secondary prevention. We also discuss recently identified methodologic challenges to the study of knee osteoarthritis, particularly osteoarthritis progression. Recent findingsRecent epidemiologic studies and systematic reviews of knee osteoarthritis have confirmed that being overweight and obese, and knee injuries increase the risk for incident knee osteoarthritis. Biomechanical risk factors such as leg-length inequalities and malalignment require further study. Obesity also appears to play a role in accelerating osteoarthritis worsening. However, with the exception of malalignment, no risk factors for knee osteoarthritis progression have been identified. Novel approaches to the study of knee pain have demonstrated a strong association between structural abnormalities and knee pain, contrary to the ‘so-called’ structure-symptom discordance, as well as between fluctuations of knee pain with changes in specific structural lesions. A number of methodologic issues, including conditioning on an intermediate stage of disease and depletion of susceptibles may explain, in part, the difficulty in identifying risk factors for knee osteoarthritis. SummaryThere is strong epidemiologic evidence that being overweight or obese and knee injury are associated with increased risk of developing knee osteoarthritis. Further study is required to confirm associations of leg-length inequality and malalignment with incident knee osteoarthritis. Few new risk factors for progression of knee osteoarthritis have been identified in the past few years. Without such knowledge, secondary prevention of osteoarthritis remains challenging.
Sustaining Quality Improvement in Community Health Centers: Perceptions of Leaders and Staff

The Health Disparities Collaboratives are the largest national quality improvement (QI) initiatives in community health centers. This article identifies the incentives and assistance personnel believe are necessary to sustain QI. In 2004, 1006 survey respondents (response rate 67%) at 165 centers cited lack of resources, time, and staff burnout as common barriers. Release time was the most desired personal incentive. The highest funding priorities were direct patient care services (44% ranked no. 1), data entry (34%), and staff time for QI (26%). Participants also needed help with patient self-management (73%), information systems (77%), and getting providers to follow guidelines (64%).
National parliamentary scrutiny of European Union affairs: explaining divergence of formal arrangements and actual activity

The role of national parliaments in the European Union (EU) has gained prominence in the last decade among academics and practitioners alike in relation to the alleged democratic deficit of the EU. The existing literature has largely focused on the formal powers of national parliaments. However, the formally strongest parliaments are not necessarily the most active, and vice versa. This thesis argues that this divergence between formal rights and actual activity exists because not formal scrutiny powers, but public Euroscepticism, the presence of parties for which Europe is salient and which care deeply about Europe (‘issue entrepreneurs’) as well as internal party cohesion on the EU are the most important factors influencing activity. Public Euroscepticism leads to more debate and resolutions when issue entrepreneurs are present and when parties have a coherent position on Europe. Recent measures and future plans to further increase the formal powers of national parliaments might not lead to more activity and might thus not help to ameliorate the democratic deficit. Methodologically, this thesis relies on computer-assisted content analysis, multilevel models as well as elite interviews. Each of the three papers focuses on a different aspect of parliamentary activity. The first paper focuses on the ‘communication function’ of parliaments and presents an analysis of parliamentary debates in EU affairs. The second paper quantitatively analyses the determinants of parliamentary activity in the form of resolutions, relating to the government control or ‘scrutiny function’. The third paper investigates parliamentary scrutiny activity in-depth in a case study of the ‘Fiscal Compact’. The thesis argues that national parliaments should mostly concentrate on their communication function in EU affairs, while other functions might best be carried out by the European Parliament. It thus suggests that different parliamentary functions can best be fulfilled by different institutions in the EU multilevel system.
On Earnings Management

The objective existence of information asymmetry and accounting uncertainty requires a rational enterprise to manage earnings.The earnings management is neutral and different from the profit manipulation.It can moderately make up the rigidity and incompleteness of contracts,and help to enhance the information of earnings and guide resource allocation prospectively.But the earnings management is still a type of accounting information distortion.Improving the stock market regulation can help to reduce the policy incentives of earnings management;strengthening the accounting principles and accounting system development can narrow the management of the space;improving corporate governance structure and raising certified public accountants occupational level can sound the administration of internal and external binding mechanism.
Admission and Acute Complication Rate for Outpatient Lumbar Microdiscectomy

Objective: Specialization is generally independently associated with improved outcomes for most types of surgery. This is the first study comparing the immediate success of outpatient lumbar microdiscectomy with respect to acute complication and conversion to inpatient rate. Long term pain relief is not examined in this study. Methods: Two separate prospective databases (one belonging to a neurosurgeon and brain tumor specialist, not specializing in spine (NS) and one belonging to four spine surgeons (SS)) were retrospectively reviewed. All acute complications as well as admission data of patients scheduled for outpatient lumbar microdiscectomy were extracted. Results: In total, 269 patients were in the NS group and 137 patients were in the SS group. The NS group averaged 24 cases per year while the SS group averaged 50 cases per year. Chi-square tests revealed no difference in acute complication rate [NS(6.7%), SS(7.3%)] (p>0.5) and admission rate [NS(4.1%), SS(5.8%)] (p=0.4) while the SS group had a significantly higher proportion of patients undergoing repeat microdiscectomy [NS(4.1%), SS(37.2%)] (p<0.0001). Excluding revision operations, there was no statistically significant difference in acute complication [NS(5.4%), SS(1.2%)] (p=0.09) and conversion to inpatient [NS(4.3%), SS(4.6%)] (p>0.5) rate. The combined acute complication and conversion to inpatient rate was 6.9% and 4.7% respectively. Conclusion: Based on this limited study, outpatient lumbar microdiscectomy can be apparently performed safely with similar immediate complication rates by both non-spine specialized neurosurgeons and spine surgeons, even though the trend favored the latter group for both outcome measures.
Dealing with immunogenicity of biologicals: assessment and clinical relevance

Purpose of reviewIn the last decade, biologicals revolutionized rheumatology. An increasing number of patients benefit from biotherapeuticals. However, some patients do not respond to treatment and others lose their response after a certain time. Immunogenicity is one of the factors linked to secondary nonresponse but its clinical significance has remained controversial. Recent findingsIn recent years, knowledge of how to assess immunogenicity of biologicals has improved. Various reports show an inverse relationship between drug levels and antibody formation against the drug. Studies associated immunogenicity of therapeutic antibodies with clinically significant nonresponse in a subgroup of patients. Clinically relevant immunogenicity is influenced by several factors including dosing and concomitant medication. It has been shown that immunogenicity against biologicals can be persistent or transient. SummaryImmunogenicity affects a significant number of patients treated with biologicals. Monitoring of drug levels as well as of antibodies against therapeutic antibodies may lead to more rational treatment strategies.
Development of peripheral hindlimb and central spinal cord innervation by subpopulations of dorsal root ganglion cells in the embryonic rat

The development of rat peripheral hindlimb and lumbar dorsal horn innervation by different subpopulations of dorsal root ganglion cells was investigated from embryonic day (E)13 to birth by using immunostaining. Antibodies to protein gene product (PGP) 9.5, growth associated protein (GAP) 43, and peripherin were used as pan‐neuronal markers, RT97 for A fibres; calcitonin gene‐related peptide (CGRP), trkA, and the lectin IB4 for small A and C fibres. Size frequency analysis showed that RT97 is a selective marker for large A cells at E18. Although both A and C fibres enter the hindlimb at E13–14, A fibres are the first to innervate the skin and dominate over small fibre innervation until later fetal life. CGRP expression in sensory axons appears at E19 and in all regions simultaneously, suggesting expression in existing fibres. All sensory terminals grow transiently to the skin surface before retracting subepidermally at late embryonic stages. The development of peripheral and central innervation by the same subpopulations of sensory neurons was compared. The entry of A fibre terminals into the lumbar dorsal horn at E14 coincided with hindlimb skin innervation. In contrast, C fibres were not detected in the dorsal horn until E18, 4 days after peripheral innervation. CGRP expression appears in both spinal cord and hindlimb targets at E19. IB4 binding in the central terminals began at E18 but was never observed in embryonic peripheral axons.These results demonstrate that in fetal skin, A fibre innervation dominates over C fibres. In addition, alathough peripheral and central innervation by A fibres coincide, this is not true for C fibres, suggesting that central target factors may control C fibre terminal growth within the dorsal horn. J. Comp. Neurol. 418:281–298, 2000. © 2000 Wiley‐Liss, Inc.
NL-InSAR: Nonlocal Interferogram Estimation

Interferometric synthetic aperture radar (SAR) data provide reflectivity, interferometric phase, and coherence images, which are paramount to scene interpretation or low-level processing tasks such as segmentation and 3-D reconstruction. These images are estimated in practice from a Hermitian product on local windows. These windows lead to biases and resolution losses due to the local heterogeneity caused by edges and textures. This paper proposes a nonlocal approach for the joint estimation of the reflectivity, the interferometric phase, and the coherence images from an interferometric pair of coregistered single-look complex (SLC) SAR images. Nonlocal techniques are known to efficiently reduce noise while preserving structures by performing the weighted averaging of similar pixels. Two pixels are considered similar if the surrounding image patches are “resembling. ” Patch similarity is usually defined as the Euclidean distance between the vectors of graylevels. In this paper, a statistically grounded patch-similarity criterion suitable to SLC images is derived. A weighted maximum likelihood estimation of the SAR interferogram is then computed with weights derived in a data-driven way. Weights are defined from the intensity and interferometric phase and are iteratively refined based both on the similarity between noisy patches and on the similarity of patches from the previous estimate. The efficiency of this new interferogram construction technique is illustrated both qualitatively and quantitatively on synthetic and true data.
Cell Loss during Pseudoislet Formation Hampers Profound Improvements in Islet Lentiviral Transduction Efficacy for Transplantation Purposes

Islet transplantation is a promising treatment in type 1 diabetes, but the need for chronic immunosuppression is a major hurdle to broad applicability. Ex vivo introduction of agents by lentiviral vectors—improving β-cell resistance against immune attack—is an attractive path to pursue. The aim of this study was to investigate whether dissociation of islets to single cells prior to viral infection and reaggregation before transplantation would improve viral transduction efficacy without cytotoxicity. This procedure improved transduction efficacy with a LV-pWPT-CMV-EGFP construct from 11.2 ± 4.1% at MOI 50 in whole islets to 80.0 ± 2.8% at MOI 5. Viability (as measured by Hoechst/PI) and functionality (as measured by glucose challenge) remained high. After transplantation, the transfected pseudoislet aggregates remained EGFP positive for more than 90 days and the expression of EGFP colocalized primarily with the insulin-positive β-cells. No increased vulnerability to immune attack was observed in vitro or in vivo. These data demonstrate that dispersion of islets prior to lentiviral transfection and reaggregation prior to transplantation is a highly efficient way to introduce genes of interest into islets for transplantation purposes in vitro and in vivo, but the amount of β-cells needed for normalization of glycemia was more than eightfold higher when using dispersed cell aggregates versus unmanipulated islets. The high price to pay to reach stable and strong transgene expression in islet cells is certainly an important cell loss.
Differentiating brown and white adipose tissues by high-resolution diffusion NMR spectroscopy[S]

There are two types of fat tissues, white adipose tissue (WAT) and brown adipose tissue (BAT), which essentially perform opposite functions in whole body energy metabolism. There is a large interest in identifying novel biophysical properties of WAT and BAT by a quantitative and easy-to-run technique. In this work, we used high-resolution pulsed field gradient diffusion NMR spectroscopy to study the apparent diffusion coefficient (ADC) of fat molecules in rat BAT and WAT samples. The ADC of fat in BAT and WAT from rats fed with a chow diet was compared with that of rats fed with a high-fat diet to monitor how the diffusion properties change due to obesity-associated parameters such as lipid droplet size, fatty acid chain length, and saturation. Feeding a high-fat diet resulted in increased saturation, increased chain lengths, and reduced ADC of fat in WAT. The ADC of fat was lower in BAT relative to WAT in rats fed both chow and high-fat diets. Diffusion of fat was restricted in BAT due to the presence of small multilocular lipid droplets. Our findings indicate that in vivo diffusion might be a potential way for better delineation of BAT and WAT in both lean and obese states.