Miracle-dz/UniFAR-FTUs huggingface dataset:
White spot syndrome virus protein ICP11: A histone-binding DNA mimic that disrupts nucleosome assembly

White spot syndrome virus (WSSV) is a large (≈300 kbp), double-stranded DNA eukaryotic virus that has caused serious disease in crustaceans worldwide. ICP11 is the most highly expressed WSSV nonstructural gene/protein, which strongly suggests its importance in WSSV infection; but until now, its function has remained obscure. We show here that ICP11 acts as a DNA mimic. In crystal, ICP11 formed a polymer of dimers with 2 rows of negatively charged spots that approximated the duplex arrangement of the phosphate groups in DNA. Functionally, ICP11 prevented DNA from binding to histone proteins H2A, H2B, H3, and H2A.x, and in hemocytes from WSSV-infected shrimp, ICP11 colocalized with histone H3 and activated-H2A.x. These observations together suggest that ICP11 might interfere with nucleosome assembly and prevent H2A.x from fulfilling its critical function of repairing DNA double strand breaks. Therefore, ICP11 possesses a functionality that is unique among the handful of presently known DNA mimic proteins.
Anemia in young children of the Muynak District of Karakalpakistan, Uzbekistan: prevalence, type, and correlates.

OBJECTIVES This study examined prevalence and correlates of anemia in the Muynak District of Uzbekistan, an area of rapidly changing social and economic conditions following the collapse of the Soviet Union. METHODS Questionnaire data and blood samples were collected on a random sample of 433 children aged 1 through 4 years. RESULTS The prevalence of anemia ranged from 89% in 1-year-olds to 48% in 4-year-olds. Correlates for anemia included younger age, a communal water source, and a history of pica. CONCLUSIONS Anemia is a widespread problem in young children in this district. An aggressive attempt to reverse this problem is needed.
Spatial-frequency channels in human vision.

Psychometric functions were determined concurrently for detection of simple gratings (luminance sinusoidally modulated with spatial frequency f) and complex gratings (luminance modulated by the sum of two sinusoids, with frequencies f and f′). Results were used to test the hypothesis that the two components of a complex grating may be detected independently. In an extensive experiment with f = 14 cycles/deg, the independence hypothesis was consistently rejected only when f/f′=54 or 45, but rarely rejected when the value of f/f′ lay outside this range. In other experiments, f was between 1.9 and 22.4 cycles/deg. All results are compatible with the assumption that the human visual system contains sensory channels, each selectively sensitive to different narrow ranges of spatial frequencies, whose outputs are detected independently.
Physical activity level is associated with the O2 cost of walking in cerebral palsy.

PURPOSE To determine, in children and adolescents with cerebral palsy (CP), the relationship between physical activity level (PAL) and i) oxygen cost of walking, and ii) peak VO2. METHODS In 11 subjects (10.6-16.3 yr) with mild CP, PAL, the ratio of total energy expenditure to resting energy expenditure, was determined from 3 d of heart rate (HR) monitoring (field), with individual HR-VO2 calibrations done in the lab. The oxygen cost of walking was measured during three 3-min walks on a treadmill at 60, 75, and 90% of each subject's fastest treadmill walking speed (FWS). Subjects also performed a maximal treadmill exercise test. Alpha was set at 0.05. RESULTS One subject was an outlier and eliminated from all simple linear regression analyses. For the remaining 10 subjects, PAL (1.37+/-0.18) was related (r=-0.70 to -0.84) to net VO2 at 60 and 75% FWS (13.1+/-4.1 and 16.2+/-4.2 mL.kg.min), net VO2.m, averaged across the three speeds (0.32+/-0.23 mL.kg.m), and percent peak VO2 at all three speeds (54.5+/-21.5, 63.5+/-20.9, and 75.5+/-15.1%). PAL was not significantly related to net VO2 at 90% FWS (20.8+/-5.3 mL.kg.min) or to peak VO2 (34.0+/-9.2 mL.kg.min). CONCLUSION For this population, those with low PAL may also have a high oxygen cost of walking. These individuals' PAL was not related to their peak VO2. Further research is required to determine whether interventions that decrease the oxygen cost of walking also affect PAL and whether changes in PAL affect the oxygen cost of walking.
Marginal zinc deficiency in older adults: responsiveness of zinc status indicators.

OBJECTIVE Suspicions that mild zinc deficiency is common among the elderly cannot be confirmed or refuted because definitive indicators of zinc status are lacking. The goal of this study was to document the clinical responsiveness of parameters of zinc status in a group of older adults consuming a carefully controlled diet: first moderately low in zinc (3.97 mg/day for 15 days) and then high in zinc (28.19 mg/day for 6 days). METHODS Fifteen older adults (mean age = 66.6 yrs) volunteered to consume a marginally zinc-deficient diet for 15 days followed by 6 days of zinc repletion. Plasma concentrations of erythrocyte metallothionein and the enzyme 5'-nucleotidase, as well as levels of zinc, alkaline phosphatase, copper and ceruloplasmin were measured before and after zinc depletion and repletion. RESULTS Plasma zinc levels were not altered during the study. Alkaline phosphatase (AP) values did not change in the expected direction, although a small decrease in AP following zinc repletion was statistically significant. Erythrocyte metallothionein results followed a pattern similar to that of alkaline phosphatase, little change, but a small, statistically significant drop after zinc repletion. As expected, there were no diet-associated changes in plasma copper and ceruloplasmin levels. In contrast, plasma concentrations of the enzyme 5'-nucleotidase decreased (p < 0.01) from 2.7 +/- 0.5 to 1.1 +/- 0.5 U during zinc depletion and increased (p < 0.05) to 2.2 +/- 0.4 U after 6 days of repletion. CONCLUSIONS Mild zinc deficiency is difficult to detect. In this study, traditional indicators such as plasma zinc and alkaline phosphatase did not change as would be expected in response to alterations in zinc intake. Likewise, erythrocyte metallothionein did not respond to altered zinc intakes as expected but this factor may reflect long-standing or more severe zinc depletion and thus requires additional study. Activity of the enzyme 5'-nucleotidase appears responsive to acute changes in zinc intake; however, more work is needed to define how well these activities will reflect zinc intake in other types of subjects.
Combined Subgaleal/Myocutaneous Technique for Temporalis Muscle Dissection

Abstract Background The frontal branch of the facial nerve (FBFN) is the most susceptible neural structure to injury during frontotemporal craniotomies. The balance between adequate temporalis muscle mobilization and frontal branch protection with minimal anatomical alteration is the philosophy behind our approach to temporalis muscle dissection. Objective To describe a combined subgaleal/myocutaneous technique for dissection and mobilization of the temporalis muscle in anterolateral cranial approaches. Methods Interdisciplinary literature review of the anatomical course of the FBFN was performed. Retrospective analysis of anterolateral craniotomies performed at our institution in which the combined subgaleal/myocutaneous (CSGMC) technique was performed. Results A total of 71 cases of anterolateral craniotomies (excluding full variant orbitozygomatic) were performed with the successful application of a CSGMC technique (36 pterional, 31 orbitopterional, and 4 fronto-orbital). Partial frontalis weakness was transient in one case. Conclusion The CSGMC technique provides sufficient protection for the FBFN and allows for adequate mobilization for a variety of skull base exposures while minimally violating myofascial anatomy. This is the first reported technique that allows both adequate temporalis muscle mobilization with performance of the one-piece orbitofrontal and orbitopterional approaches, without disruption of the superficial/deep temporalis fascia and fat-pad complex.
High efficiency design of induction machines for industrial applications

This paper presents a high-level overview of the issues and challenges relating to high efficiency induction machine design for industrial applications. First, a brief overview of the classical approach to machine design is presented, followed by a commentary on contemporary design using finite element method and design optimization techniques. The issues, challenges and opportunities of improving the efficiency of induction motors are discussed and some interesting results of stack lengthening investigations are presented.
Bacillus cereus G9241 Makes Anthrax Toxin and Capsule like Highly Virulent B. anthracis Ames but Behaves like Attenuated Toxigenic Nonencapsulated B. anthracis Sterne in Rabbits and Mice

ABSTRACT Bacillus cereus G9241 was isolated from a welder with a pulmonary anthrax-like illness. The organism contains two megaplasmids, pBCXO1 and pBC218. These plasmids are analogous to the Bacillus anthracis Ames plasmids pXO1 and pXO2 that encode anthrax toxins and capsule, respectively. Here we evaluated the virulence of B. cereus G9241 as well as the contributions of pBCXO1 and pBC218 to virulence. B. cereus G9241 was avirulent in New Zealand rabbits after subcutaneous inoculation and attenuated 100-fold compared to the published 50% lethal dose (LD50) values for B. anthracis Ames after aerosol inoculation. A/J and C57BL/6J mice were comparably susceptible to B. cereus G9241 by both subcutaneous and intranasal routes of infection. However, the LD50s for B. cereus G9241 in both mouse strains were markedly higher than those reported for B. anthracis Ames and more like those of the toxigenic but nonencapsulated B. anthracis Sterne. Furthermore, B. cereus G9241 spores could germinate and disseminate after intranasal inoculation into A/J mice, as indicated by the presence of vegetative cells in the spleen and blood of animals 48 h after infection. Lastly, B. cereus G9241 derivatives cured of one or both megaplasmids were highly attenuated in A/J mice. We conclude that the presence of the toxin- and capsule-encoding plasmids pBCXO1 and pBC218 in B. cereus G9241 alone is insufficient to render the strain as virulent as B. anthracis Ames. However, like B. anthracis, full virulence of B. cereus G9241 for mice requires the presence of both plasmids.
Effects of Self-Evaluation on the P300 Event-Related Potential

The effects of self evaluation on the P300 event-related potential (ERP) were explored with 56 participants (16 men, 40 women; M age = 23.4 yr., SD = 1.2) across three conditions. The conditions included (1) a standard ERP auditory oddball discrimination between a random target (15% occurrence) and standard stimuli (85% occurrence), (2) the oddball task followed by the additional cognitive task of maintaining a mental count of the target tones, and (3) the oddball task followed by the additional cognitive task of self-evaluating whether they felt surprised by the current occurrence of the target tone. The added cognitive requirements for Conditions 2 and 3 required the subjects to maintain a cognitive readiness for the secondary stimulus-related task during their sensory discrimination response for the standard oddball task. During the self-evaluation condition, the P300 amplitude was significantly larger across all recording locations than the regular oddball condition and the cognitive count condition.
Variability of polymorphic families of three types of xylanase inhibitors in the wheat grain proteome

Cereals contain proteinaceous inhibitors of endo‐β‐1,4‐xylanases (E.C.3.2.1.8, xylanases). Since these xylanase inhibitors (XIs) are only active against xylanases of microbial origin and do not interact with plant endogenous xylanases, they are believed to act as a defensive barrier against phytopathogenic attack. So far, three types of XIs have been identified, i.e. Triticum aestivum XI (TAXI), xylanase inhibiting protein (XIP), and thaumatin‐like XI (TLXI) proteins. In this study the variation in XI forms present in wheat grain was elucidated using high‐resolution 2‐DE in combination with LC‐ESI‐MS/MS and biochemical techniques. Reproducible 2‐DE fingerprints of TAXI‐, XIP‐, and TLXI‐type XIs, selectively purified from whole meal of three European wheat cultivars using cation exchange chromatography followed by affinity chromatography, were obtained using a pH‐gradient of 6 to 11 and a molecular mass range of 10 to 60 kDa. Large polymorphic XI families, not known to date, which exhibit different pI‐ and/or molecular mass values, were visualised by colloidal CBB staining. Identification of distinct genetic variants by MS/MS‐analysis provides a partial explanation for the observed XI heterogeneity. Besides genetic diversity, PTMs, such as glycosylation, account for the additional complexity of the 2‐DE patterns.
Profiles and Service Utilization for Children Accessing a Mental Health Walk-In Clinic versus Usual Care

Many children and adolescents with mental health problems do not receive the treatment they need. Unmet need raises questions about specific barriers that may prevent service use, and/or the characteristics of children and families who are less likely to receive care. Brief interventions or single–session psychotherapy delivered in a highly accessible manner are methods of addressing the problems associated with waitlists and limited access to care. In the current study the authors offer an exploratory evaluation of the West End Walk-In Counseling Centre for children and youth with psychosocial problems. Children 4 to 18 years of age who accessed the Walk-In Counseling Centre and a comparison group of clients who accessed usual care were assessed at intake, post-treatment, and 3-month follow-up on demographic characteristics, behavioral/emotional adjustment and functioning, client satisfaction, and service use. Children in the walk-in group had more severe behavioral/emotional adjustment and functioning than usual care clients at baseline. At post-treatment, walk-in clients had lower scores on Total Mental Health Problems and Internalizing Behaviors, and exhibited fewer problems across all scales at follow-up. Walk-in clients found the wait time for service more reasonable and at follow-up, felt the service addressed concerns and had higher regard for counselor availability and cultural sensitivity of the service than usual care clients. Service utilization, assessed at post–treatment and 3-month follow-up, showed that both groups were more likely to access mental health and education services rather than health or child welfare services, and were more likely to have used services in the 12 months prior to service than the 3 months following service completion. Walk–in clients had steeper rates of improvement compared to usual care clients despite equivalence in psychosocial functioning at baseline. The walk-in model may be an effective alternative to usual care, particularly for those clients only willing to wait up to 2 weeks for service.
Assessment of Implicit Anti-Fat and Pro-Slim Attitudes in Young Women Using the Implicit Relational Assessment Procedure

Research on implicit attitudes to both male and female body size has produced evidence for the existence of an attitudinal bias in the form of preference for images of thinness over images of fatness. Specifically, studies that have employed the Implicit Relational Assessment Procedure (IRAP) have shown that this bias is specifically attributable to a pro-slim attitude, rather than to an anti-fat one. It is not clear, however, if the same type of specific pro-slim bias will be found when attitudes are measured exclusively for female participants and only to female targets. The present study has employed the IRAP for the assessment of implicit attitudes towards fatness and slimness in a sample of 40 Spanish young female college students. The task required participants to respond relationally in alternating trial-blocks. In pro-slim/anti-fat blocks, they had to respond as if photos of underweight women were pleasant and photos of overweight women were unpleasant. In anti-fat/pro-slim blocks they had to respond according to the opposite pattern (overweight as pleasant and underweight as unpleasant). Additionally, participants had to produce explicit ratings of pleasantness for the same photos used in the IRAP (with a visual analogue scale: VAS), and answer measures of body dissatisfaction (the Body Shape Questionnaire: BSQ) and of eating disorders symptomatology (the Eating Attitudes Test: EAT-40). Results showed no evidence of implicit bias for body size (neither pro-slim nor anti-fat). Besides, no significant correlations were found between implicit and explicit measures. These results are consistent with prior evidence suggesting that women show less anti-fat prejudice than men, and with studies that find less anti-fat bias in Spanish population than in Anglo-Saxon population.
Abnormal fecal Lactobacillus flora and vitamin B12 deficiency in a patient with short bowel syndrome.

Lactobacillus overgrowth has been reported in patients with short bowel syndrome (SBS) (1–4). We previously reported D-lactic acidosis that Lactobacillus overgrowth caused in a male SBS patient (5). He had severe anemia related to vitamin B12 deficiency 9 years later. It has been generally accepted that B12 deficiency in SBS patients results from the inadequate absorption of B12 and/or the bacteria–host competition for uptake of B12 (6). In the present study, we suspected the recurrence of Lactobacillus overgrowth as a possible cause, because some lactobacilli require B12 for growth. Therefore, we analyzed the fecal flora and the B12 auxotrophy of Lactobacillus isolates.
Increased Efficacy of the Immunoglobulin G2a Subclass in Antibody-Mediated Protection against Lactate Dehydrogenase-Elevating Virus-Induced Polioencephalomyelitis Revealed with Switch Mutants

ABSTRACT Immunoglobulin G1 (IgG1), IgG2a, and IgG2b switch variants were derived from an IgG3 monoclonal antibody directed against the VP3 envelope glycoprotein of lactate dehydrogenase-elevating virus (LDV). Among the four antibodies, IgG2a delayed the onset and progression of LDV-induced polioencephalomyelitis more than did the other subclasses. This suggests that the IgG2a predominance observed in many IgG antibody responses elicited by live viruses could, at least under some circumstances, correspond to the selection of the best protection for the infected host.
College and Career Readiness: Course Taking Of Deaf and Hard of Hearing Secondary School Students

Research shows that deaf and hard of hearing (DHH) students frequently enter college and the workplace relatively unprepared for success in math, science, and reading. Based on data from the National Longitudinal Transition Study–2 (NLTS2), the present study focused on DHH students’ college and career readiness by investigating their opportunities in secondary school to acquire college and career skills. DHH students earned more credits overall than hearing peers; both groups earned a similar number of credits in academic courses. However, DHH students took more vocational and nonacademic courses and fewer courses in science, social science, and foreign languages. There was evidence that DHH students’ academic courses in math lacked the rigor of those taken by hearing peers, as DHH students earned more credits in basic math and fewer credits in midlevel math courses, and even fewer in advanced math courses, than hearing peers.
Temperature Dependency of T1 Relaxation Time in Unfixed and Fixed Human Brain Tissue

MR relaxation times of most tissues are ex- pected to depend on temperature which can impact find- ings in post mortem MRI or MRI based thermometry. The purpose of this study was to investigate the exact tem- perature dependency of the T1 relaxation in different structures in unfixed and fixed human brain samples. Following autopsy, coronal brain slices from five de- ceased subjects underwent MRI at 3T in a temperature range between 4°C and 37°C. T1 showed a linear depend- ency on temperature with the highest temperature coeffi- cient in the cortex (17 ms/°C) and the lowest in the white matter (3ms/°C). The fixation of the tissue lowered T1 and additionally the temperature coefficient in all regions investigated. The temperature coefficients obtained in this study serve as reference for thermometry and for correct- ing quantitative post mortem MRI.
Detecting coagulability status by thromboelastography in women with the history of preeclampsia and inherited thrombophilia.

OBJECTIVE To assign tendency to thrombosis in patients with preeclampsia and inherited thrombophilia using thromboelastography (TEG), and therefore to evaluate possible relationship between thrombophilia and preeclampsia. MATERIALS AND METHODS Kinetics of clot formation was assessed with TEG analyzer in 49 patients with severe preeclampsia, 54 cases with previous diagnosis of inherited thrombophilia, and 31 controls. RESULTS 'r, 'k', TMA, coagulation index (CI) parameters were found statistically discrete between patients with inherited thrombophilia and controls. The difference between preeclampsia and control groups was not statistically significant. The difference in a angle was statistically significant between thrombophilics and preeclamptics (p = 0.01), and between thrombophilics and controls (p = 0.004). CI was found statistically lower in thrombophilia group than control group (p = 0.006). Particularly, clot lysis time (CLT) was measured to shorten in preeclampsia when compared with controls and patients with thrombophilia (p = 0.032, p = 0.028, respectively). CONCLUSIONS Not only the inherited thrombophilia group but also preeclampsia group demonstrated elongated clot initiation patterns when compared to the controls. Moreover, apart from the patients with inherited thrombophilia, preeclamptics exposed shorter CLT values indicating a possible increment in clot turn over, which eventually results in increased depletion of coagulation substrates, and thus, increased frequencies of oxidative cycle injury.
The Psychological Distance of Climate Change

Avoiding dangerous climate change is one of the most urgent social risk issues we face today and understanding related public perceptions is critical to engaging the public with the major societal transformations required to combat climate change. Analyses of public perceptions have indicated that climate change is perceived as distant on a number of different dimensions. However, to date there has been no in‐depth exploration of the psychological distance of climate change. This study uses a nationally representative British sample in order to systematically explore and characterize each of the four theorized dimensions of psychological distance—temporal, social, and geographical distance, and uncertainty—in relation to climate change. We examine how each of these different aspects of psychological distance relate to each other as well as to concerns about climate change and sustainable behavior intentions. Results indicate that climate change is both psychologically distant and proximal in relation to different dimensions. Lower psychological distance was generally associated with higher levels of concern, although perceived impacts on developing countries, as an indicator of social distance, was also significantly related to preparedness to act on climate change. Our findings clearly point to the utility of risk communication techniques designed to reduce psychological distance. However, highlighting the potentially very serious distant impacts of climate change may also be useful in promoting sustainable behavior, even among those already concerned.