Miracle-dz/UniFAR-FTUs huggingface dataset:
Propofol infusion anaesthesia and immune response in minor surgery

This study was set up to evaluate the effects of propofol infusion anaesthesia on immunological function in minor surgery. Twenty‐seven patients (median age 51 years, ASA 1‐2) scheduled for minor breast surgery were randomly assigned to two groups. Anaesthesia was induced in group 1 with propofol 2.5 mg.kg‐1 and maintained with propofol 12 mg.kg‐1.h‐1 and 30% O2 in air, whereas in group 2 anaesthesia was induced with thiopentone 4 mg.kg‐1 and maintained with 70% N2O in O2. Fentanyl and vecuronium were used in both groups. The percentages of T cells (p < 0.001), B cells (p < 0.01) and memory T cells increased (p < 0.01) in both groups. T helper cell percentages increased in the propofol but not in the thiopentone group (p < 0.05). The percentages of natural killer cells decreased from pre‐induction values in both groups (p < 0.001). No changes were seen in lymphocyte proliferative responses. Minor breast surgery under propofol or conventional combined anaesthesia had only minor effects on the immune response. The higher percentage of T helper cells after propofol anaesthesia compared to conventional combined anaesthesia is beneficial, but its clinical importance remains to be determined.
Magnetic resonance imaging evaluation of temporomandibular joint derangement in symptomatic and asymptomatic patients.

OBJECTIVE To highlight the importance of MRI in evaluation of the tempromandibular joint (TMJ). METHODS The MRI examination was performed at King Khalid University Hospital, Riyadh, Saudi Arabia with the approval from the local ethics committee, on 34 patients (68 joints) between January 2006 and November 2007, in which 10 were considered asymptomatic subjects (control). The remaining had symptoms and signs of TMJ pain or dysfunction, including limitation of movement and clicking. All our subjects were examined in both closed and open mouth position. Images were obtained by 1.5-T MRI system, in oblique sagittal plane utilizing 3 pulse sequences including T1 and T2 spin-echo, and spoiled gradient recall sequences. The evaluation of the meniscal disc configuration and position was carried out by 2 radiologists. RESULTS Sixteen out of 20 joints of asymptomatic subjects were normal. In the other 2 asymptomatic subjects, the MRI showed anterior disc displacement with reduction in 3 joints, and degenerative changes in 2 joints. Out of the 48 symptomatic joints, 26 (45%) joints were considered normal, while the other 22 joints showed anterior dislocation without reduction. Degenerative joint disease was also seen in 18 joints. CONCLUSION The MRI with the use of surface coils markedly improves the delineation of internal derangement of the TMJ, therefore, it enhanced the capability of detecting certain abnormalities, which proved to have a statistical significance in symptomatic patients.
Theoretical analysis of voltage-gated membrane channels under GSM and DECT exposure

In this work the response of a theoretical model of voltage-dependent membrane channels is analyzed for EM fields used in GSM and DECT cellular phones. A comparison between the effects of the two different communication protocols is performed, as well as an evaluation of the response of the model to temperature variations. In the simulations the conditions of exposure to EM fields generated by GSM and DECT mobile equipments, has been set starting from existing safety standards. Microscopic effects are investigated at the ionic channel's level inside cellular membranes. The obtained results show that both GSM and DECT signals have an evident effect on the behaviour of ionic channels, and seem to induce nonthermal effects. Maximum variations of around 30% in the open probability have been observed for sodium and potassium channels, and around 60% for calcium. The DECT signal seems to be more perturbing than the GSM signal on the calcium channel.
Effects of Zolpidem CR on Sleep and Nocturnal Ventilation in Patients with Heart Failure.

STUDY OBJECTIVE This study aimed to evaluate the effects of zolpidem CR (controlled release) on sleep and nocturnal ventilation in patients with congestive heart failure, a population at risk for insomnia and poor sleep quality. METHODS Fifteen patients with heart failure (ischemic cardiomyopathy) and ejection fraction ≤ 45% in NYHA functional class I or II were evaluated with full polysomnography in a placebo-controlled, double-blind, randomized trial. Patients underwent three tests: (1) baseline polysomnography and, after randomization, (2) a new test with zolpidem CR 12.5 mg or placebo, and after 1 week, (3) a new polysomnography, crossing the "medication" used. RESULTS A 16% increase in total sleep time was found with the use of zolpidem CR and an increase in stage 3 NREM sleep (slow wave sleep). The apnea hypopnea index (AHI) did not change with zolpidem CR even after controlling for supine position; however, a slight but significant decrease was observed in lowest oxygen saturation compared with baseline and placebo conditions (83.60 ± 5.51; 84.43 ± 3.80; 80.71 ± 5.18, P = 0.002). CONCLUSION Zolpidem CR improved sleep structure in patients with heart failure, did not change apnea hypopnea index, but slightly decreased lowest oxygen saturation.
Minimum information about animal experiments: supplier is also important

It has now been established that functional recovery after spinal cord injury (SCI) depends on several parameters, including animal strain. Here we demonstrate that rats from the same strain (Wistar) but from two independent commercial suppliers present different motor, sensory, and autonomic outcomes after a standard model of SCI, the so‐called compression model. Recovery is correlated with the extension of the lesion, and we show that the vertebral canal diameter varies between the two suppliers. To substantiate this point, we carried out another set of experiments, with the so‐called contusion model, which requires bone ablation and thus whose extension is not related to vertebral canal diameter. We show that there is no difference between the two suppliers. The purpose of our communication is to alert researchers on how crucial it is to control experimental parameters as closely as possible and to establish a standard for animal experiment in order to avoid unexpected biases. © 2008 Wiley‐Liss, Inc.
Hormone receptor status of breast tumors in black, Hispanic, and non‐Hispanic white women: An analysis of 13,239 cases

It has been postulated that different biologic characteristics of a tumor might account for at least a portion of the disparity in breast cancer survival for patients across racial and ethnic groups. The hormone receptor status of breast tumors is one characteristic with prognostic significance. Results of several studies indicate a higher proportion of estrogen receptor negative (ER‐) breast tumors among black or Hispanic women compared with non‐Hispanic whites. We investigated whether the association between race and ethnicity and the joint combination of ER and progesterone receptor (PR) status was independent of age and other tumor characteristics at diagnosis.
Transverse-mode dynamics in directly modulated vertical-cavity surface-emitting lasers with optical feedback

Vertical-cavity surface-emitting lasers (VCSELs) with optical feedback are known to exhibit different transverse-mode regimes depending on the injection current. Close to threshold a VCSEL operates on the fundamental transverse mode, while for larger injection the dynamics is often multimode, with the optical feedback inducing either in-phase or anti-phase transverse mode oscillations. In this paper, we study numerically the influence of current modulation on these different feedback-induced transverse-mode regimes. The modulation amplitude and period are taken as control parameters. We find that the in-phase and anti-phase regimes are robust under weak modulation. As the modulation amplitude increases, there is a transition to a dynamics governed by the current modulation, where the total output power follows the injection current and there is either single-mode or in-phase multimode behavior. However, the effect of the current modulation depends on the modulation period. Under fast modulation, the laser cannot follow the modulation and the optical-feedback-induced effects are dominant. On the contrary, under slow modulation there is a superposition of modulation and feedback effects, with the total output following the modulated current and an underlying transverse-mode behavior mainly determined by the optical feedback. A resonant behavior was observed for modulation periods close to the internal oscillation period. In this case, current modulation induces pulsing output intensity with single-mode or in-phase multimode behavior.
Prevention of Radiation-Induced Oral Cavity Mucositis by Plasmid/Liposome Delivery of the Human Manganese Superoxide Dismutase (SOD2) Transgene

Abstract Guo, H. , Seixas-Silva, Jr., J. A. , Epperly, M. W. , Gretton, J. E. , Shin, D. M. , Bar-Sagi, D. , Archer, H. and Greenberger, J. S. Prevention of Radiation-Induced Oral Cavity Mucositis by Plasmid/Liposome Delivery of the Human Manganese Superoxide Dismutase (SOD2) Transgene. Radiat. Res. 159, 361–370 (2003). Oral cavity mucositis is a major toxicity of radiation therapy for head and neck cancer. In the present mouse model studies, we evaluated intraoral administration of SOD2-PL complexes 24 h before single-fraction 30-Gy irradiation for the prevention of oral cavity mucositis. Expression of the human SOD2 transgene in the oral cavity of C3H/HeNsd mice was demonstrated by nested reverse transcriptase polymerase chain reaction (RT-PCR). Mice treated intraorally with bacterial β-galactosidase gene-plasmid/liposome (LacZ-PL) or hemagglutinin (HA)-manganese superoxide dismutase-plasmid/liposome (HA-SOD2-PL) demonstrated LacZ or HA-SOD2 expression, respectively, 24 h after injection. In a second strain of mouse, SOD2-PL-treated female athymic nude mice demonstrated significantly decreased ulceration at day 5 after 30 Gy, compared to LacZ-PL-injected, irradiated mice or irradiated controls. No further reduction in radiation-induced ulceration was detected in mice treated with both SOD2-PL and 10 mg/kg of amifostine (WR-2721) 30 min before 30 Gy compared to SOD2-PL alone. No significant protection of orthotopically transplanted murine squamous cell carcinoma (SCC-VII) tumors was detected in mice that received SOD2-PL treatment before 18 Gy. Thus overexpression of human SOD2 in the oral cavity mucosa can prevent radiation-induced mucositis with no detectable compromise in the therapeutic response of orthotopically transplanted tumors.
Polyphenol, antioxidant and antimicrobial potential of six different white and red wine grape processing leftovers.

BACKGROUND During winemaking, grape polyphenols are only partly extracted, and consequently unexploited. The main aim was to characterize the phenolic content of freeze-dried grape skin and seed (FDSS) extracts obtained from Slovenian and international grape varieties and to evaluate their antioxidant, antimicrobial and anti-adhesive activities. RESULTS FDSS of six Vitis vinifera L. grapevine cultivars from Vipava Valley region (Slovenia) underwent extraction and sonification under different conditions. Flavonols were the predominant content of extracts from white 'Zelen' and 'Sauvignon Blanc' grape varieties, with strong antimicrobial activities against Gram-negative bacteria. 'Pinot Noir' FDSS extracted with 50% aqueous ethanol extraction produced a high phenolic content in the final extract, which was further associated with strong antioxidant and antimicrobial activities against all tested bacteria. Bacterial adhesion to stainless steel surfaces with minimal and maximal surface roughness was significantly inhibited (up to 60%) across a wide FDSS concentration range, with lower concentrations also effective with two types of stainless steel surfaces. CONCLUSION FDSS extracts from winery by-products show interesting phenolic profiles that include flavonols, catechins, anthocyanins and hydroxycinnamic acids, with yields influenced by grapevine cultivar and extraction conditions. The antioxidant, antimicrobial and anti-adhesive activities of 50% aqueous ethanol 'Pinot Noir' FDSS extract reveals potential applications in food, pharmaceutical and cosmetic industries for these bioactive residues. © 2016 Society of Chemical Industry.
Prolonged overall survival in gastric cancer patients after adoptive immunotherapy.

AIM To assess the efficacy of immunotherapy with expanded activated autologous lymphocytes (EAALs) in gastric cancer. METHODS An observational study was designed to retrospectively analyze the clinical data of 84 gastric cancer patients, of whom 42 were treated by EAAL immunotherapy plus conventional treatment and another 42 only received conventional treatment (control group). EAALs were obtained by proliferation of peripheral blood mononuclear cells from patients followed by phenotype determination. Clinical data including age, gender, clinical stage, chemotherapeutic regimens, hospitalization, surgical, radiotherapy, and survival data were collected along with EAAL therapy details and side effects. Patients were followed and the relationship between treatment and overall survival (OS) data obtained for the immunotherapy and control groups were compared retrospectively. The safety of EAAL immunotherapy was also evaluated. RESULTS After in vitro culture and proliferation, the percentages of CD3+, CD3+CD8+, CD8+CD27+, CD8+CD28+, and CD3+CD16+/CD56+ cells increased remarkably (P < 0.05), while the percentages of CD3+CD4+, CD4+CD25+, and CD3-CD16+/CD56+ (natural killer cells) were overtly decreased (P < 0.05); no significant change was observed in CD4+CD25+CD127- cells (P = 0.448). Interestingly, OS in the immunotherapy group was significantly higher than that in the control group, with 27.0 and 13.9 mo obtained for the two groups, respectively (P = 0.028, HR = 0.573, 95%CI: 0.347-0.945). These findings indicated a 42.7% decrease in the risk of death. In addition, we found that clinical stage and application of EAAL immunotherapy were independent prognostic factors for gastric cancer patients. Indeed, the OS in stage IIIc and IV patients that had received surgery was prolonged after EAAL immunotherapy (P < 0.05). Importantly, in vitro induction and proliferation of EAAL were easy and biologically safe. CONCLUSION Overall, EAAL adoptive immunotherapy might prolong the OS in gastric cancer patients.
Kinetoplastid Membrane Protein-11 DNA Vaccination Induces Complete Protection against Both Pentavalent Antimonial-Sensitive and -Resistant Strains of Leishmania donovani That Correlates with Inducible Nitric Oxide Synthase Activity and IL-4 Generation: Evidence for Mixed Th1- and Th2-Like Responses 

The emergence of an increasing number of Leishmania donovani strains resistant to pentavalent antimonials (SbV), the first line of treatment for visceral leishmaniasis worldwide, accounts for decreasing efficacy of chemotherapeutic interventions. A kinetoplastid membrane protein-11 (KMP-11)-encoding construct protected extremely susceptible golden hamsters from both pentavalent antimony responsive (AG83) and antimony resistant (GE1F8R) virulent L. donovani challenge. All the KMP-11 DNA vaccinated hamsters continued to survive beyond 8 mo postinfection, with the majority showing sterile protection. Vaccinated hamsters showed reversal of T cell anergy with functional IL-2 generation along with vigorous specific anti-KMP-11 CTL-like response. Cytokines known to influence Th1- and Th2-like immune responses hinted toward a complex immune modulation in the presence of a mixed Th1/Th2 response in conferring protection against visceral leishmaniasis. KMP-11 DNA vaccinated hamsters were protected by a surge in IFN-γ, TNF-α, and IL-12 levels along with extreme down-regulation of IL-10. Surprisingly the prototype candidature of IL-4, known as a disease exacerbating cytokine, was found to have a positive correlation to protection. Contrary to some previous reports, inducible NO synthase was actively synthesized by macrophages of the protected hamsters with concomitant high levels of NO production. This is the first report of a vaccine conferring protection to both antimony responsive and resistant Leishmania strains reflecting several aspects of clinical visceral leishmaniasis.
Evaluation of an outpatient protocol in the treatment of canine parvoviral enteritis

OBJECTIVE To compare 2 treatment protocols (standard in-hospital versus modified outpatient) in affecting the duration of treatment or survival of dogs with parvoviral enteritis. DESIGN Prospective, randomized study. SETTING University teaching hospital. ANIMALS Client-owned dogs with naturally acquired parvovirus were randomized to receive either an inpatient (n = 20) or outpatient (n = 20) treatment protocol. INTERVENTIONS Both groups received intravenous (IV) fluid resuscitation and correction of hypoglycemia at hospital admission. Following stabilization, basic inpatient interventions included administration of IV fluids, administration of cefoxitin (22 mg/kg IV q 8 h), and maropitant (1 mg/kg IV q 24 h). Basic outpatient interventions (provided in-hospital) included administration of subcutaneous (SC) fluid (30 mL/kg q 6 h), administration of maropitant (1 mg/kg SC q 24 h) and cefovecin (8 mg/kg SC once). Using daily electrolyte and glucose evaluations, dextrose and potassium supplementation was provided intravenously (inpatients) or orally (outpatients) as indicated. Rescue criteria were used in both groups for analgesia and nausea. All dogs were syringe fed a commercial canine convalescence diet (1 mL/kg PO q 6 h) until voluntary appetite returned. MEASUREMENTS AND MAIN RESULTS Protocol success, defined as survival to hospital discharge, was 90% (18/20) for the inpatient group compared to 80% (16/20) for the outpatient group (P = 0.66). There was no difference detected in duration of hospitalization for inpatient dogs (4.6 ± 2 days) versus outpatient dogs (3.8 ± 1.8 days, P = 0.20). Metabolic disturbances were frequent in the outpatient group, with 50% of dogs requiring dextrose supplementation and 60% of dogs requiring potassium supplementation. CONCLUSIONS An outpatient protocol may be a reasonable alternative for dogs that cannot receive standard in-hospital treatment for parvoviral enteritis. Diligent supportive care and monitoring are still required to optimize treatment of dogs with parvoviral enteritis in an outpatient setting.
Phenotypic screening approaches for Chagas disease drug discovery

ABSTRACT Introduction: Chagas disease, caused by the parasite Trypanosoma cruzi, is a global public health issue. Current treatments targeting the parasite are limited to two old nitroheterocyclic drugs with serious side effects. The need for new and safer drugs has prompted numerous drug discovery efforts to identify compounds suitable for parasitological cure in the last decade. Areas covered: Target-based drug discovery has been limited by the small number of well-validated targets – the latest example being the failure of azoles, T. cruzi CYP51 inhibitors, in proof-of-concept clinical trials; instead phenotypic-based drug discovery has become the main pillar of Chagas R&D. Rather than focusing on the technical features of these screening assays, the authors describe the different assays developed and available in the field, and provide a critical view on their values and limitations in the screening cascade for Chagas drug development. Expert opinion: The application of technological advances to the field of Chagas disease has led to a variety of phenotypic assays that have not only changed the disease discovery landscape but have also helped us to gain a better understanding of parasite/host interactions. Recent examples of target resolution from phenotypic hits will uncover new opportunities for drug discovery for Chagas disease.
The effect of hydrothermal spark discharge anodization in the early integration of implants in sheep sinuses.

OBJECTIVES Spark discharge anodic oxidation forms a porous oxide film on titanium implant surfaces, which may increase surface roughness and enhance early osseointegration. This study aimed to clinically and histomorphometric compare commercially-available sandblasted (RBM) implants, treated with hydrothermal anodization and placed into an animal maxillary sinus model. MATERIALS AND METHODS Thirty 3.75 mm × 8.5 mm threaded titanium implants were placed into the maxillary sinuses of 10 sheep via an external approach, with three test groups and 10 implants per group: right side, Control = CP-titanium with RBM surface, Test group 1 = CP-titanium with RBM + anodized surface; left side, Test group 2 = Ti-6Al-7Nb with RBM + anodized surface. Schneiderian membranes were elevated but not bone grafted. Resonant frequency analysis (RFA) was measured at surgery. Animals were sacrificed after 1 month unloaded healing. Resin-embedded undemineralized ground-sections were digitised, and mean bone-implant contact (% BIC) was measured bilaterally for the best-three consecutive threads. RESULTS Seven of 30 implants showed signs of failure. RFA was low at placement but did not differ between the groups (group mean ISQ values ranged from 23 to 35; χ(2) = 0.37). RFA was not repeated at sacrifice due to implant instability. Histomorphometric analysis showed % BIC was highest for control (34.8 ± 15.7), followed by Test 1 (29.6 ± 18.1) and Test 2 implants (23.3 ± 22.7), but this difference was not statistically significant (χ(2) = 0.3). DISCUSSION AND CONCLUSIONS Early integration of RBM implants placed into thin maxillary sinus walls was not enhanced by hydrothermal anodization of implant surfaces. This may be related to the initial low stability of the implants and the relatively short healing period. However, non-anodized RBM surfaces showed promising results, with % BIC values comparable to the best estimates of other studies using sinus grafting. Whether the modification of the implant surfaces through anodization with simultaneous sinus grafting would promote enhanced early osseointegration, is a subject of future research.
Topographic organization of the dorsal nucleus of the lateral lemniscus in the cat

The dorsal nucleus of the lateral lemniscus (DNLL) is an auditory structure of the brainstem. It plays an important role in binaural processing and sound localization and it provides the inferior colliculus with an inhibitory projection. The DNLL is a highly conserved auditory structure across mammals, but differences among species in its detailed organization have been reported.