Miracle-dz/UniFAR-FTUs huggingface dataset:
Group A Rotaviruses Produce Extrahepatic Biliary Obstruction in Orally Inoculated Newborn Mice

ABSTRACT: Extrahepatic biliary atresia is a devastating disease occurring in 1 in 10 000 to 14 000 infants annually in the United States. We have recently described preliminary data suggesting an association of group C rotavirus with biliary atresia in two infants. However, a group C rotavirus animal model of biliary atresia is not presently available. On the other hand, some strains of the bettercharacterized and much more common group A rotaviruses produce hepatobiliary disease in infant mice. This disease shares many characteristics of the human infection. The present report describes extrahepatic biliary obstruction in immunocompetent BALB/c infant mice infected with a human or animal strain of group A rotavirus. Two-d-old BALB/c mice orally inoculated with hepatobiliary tropic rotavirus were shown to have active virus replication in the biliary tract and liver as early as 48 h postinoculation. At approximately 7 d postinoculation, between one fourth and one half of infant mice, depending on the virus strain, showed signs of inflammation and swelling in the bile ducts. The obstruction was complete in about one half of symptomatic animals. Although there was no obvious atresia as described in human infants, the obstruction was irreversible about 50% of the time, and the resulting fibrosis and bile ductular proliferation in the liver were strikingly similar to those seen in the liver of the human infant with biliary atresia.
Effect of Ship-Stack Effluents on Cloud Reflectivity

Under stable meteorological conditions the effect of ship-stack exhaust on overlying clouds was detected in daytime satellite images as an enhancement in cloud reflectivity at 3.7 micrometers. The exhaust is a source of cloud-condensation nuclei that increases the number of cloud droplets while reducing droplet size. This reduction in droplet size causes the reflectivity at 3.7 micrometers to be greater than the levels for nearby noncontaminated clouds of similar physical characteristics. The increase in droplet number causes the reflectivity at 0.63 micrometer to be significantly higher for the contaminated clouds despite the likelihood that the exhaust is a source of particles that absorb at visible wavelengths. The effect of aerosols on cloud reflectivity is expected to have a larger influence on the earth's albedo than that due to the direct scattering and absorption of sunlight by the aerosols alone.
Variability of candidate genes for cardiovascular risk in the Mediterranean

A screening of 22 DNA polymorphisms has been performed in western Mediterranean populations. The analyzed markers correspond to polymorphic sites in several candidate genes for cardiovascular disease including apolipoproteins and their receptor, genes implied in the hemostasis regulation and the angiotensin converting enzyme gene. The results showed a significant degree of interpopulation diversity, in agreement with previous data from other polymorphisms. The genetic distance pattern is compatible with a south-to-north African influence in the Iberia Peninsula and a remarkable gene flow sub-Saharan Africa into Morocco.
Missed appointments at public hospitals in Riyadh, Saudi Arabia.

OBJECTIVE Failure to keep appointments poses many problems for patients, physicians and health personnel. The objective of the study is to identify factors influencing appointment failure in Riyadh Central Hospital, Riyadh, Kingdom of Saudi Arabia. METHODS The study sample consisted of 500 Saudi patients selected randomly from Riyadh Central Hospital's records. Two hundred and fifty were selected from patients who broke at least one appointment and another 250 were selected from patients who have a history of keeping records. Interviews were carried out by telephone using a structured questionnaire, only 426 patients consented to participate in the study. RESULTS The study showed that appointment failure rate is 30%. The data revealed no associations between missed appointment, behavior and age, income, travel time and method of transportation. None of the health related variables investigated were associated with missed appointments. The breakers stated waiting time before seeing a doctor, time from scheduling to appointments, transportation (particularly for women) and forgetfulness as the main reasons for missing appointments. CONCLUSION Broken appointments can be reduced by continuous health education for patients, improving efficiency of health administrators, telephone reminders and by physicians negotiating a follow-up schedule for their patients.
Job satisfaction and intention to leave: a questionnaire survey of hospital nurses in Shanghai of China.

AIM To explore nurses' views and experiences regarding job satisfaction and their intention to leave in Shanghai. BACKGROUND The widespread nursing shortage and nurses' high turnover rate has become a global issue. Job satisfaction is the most consistent predictor of nurses' intention to leave and has been reported as explaining most of the variance on their intention to leave. DESIGN A cross-sectional survey. METHODS A convenience sample of nurses was recruited from 19 large general hospitals in Shanghai and the research assistants distributed 2850 questionnaires to nurses. The response rate of this study was 78·95%, and 2250 nurses completed the questionnaire. RESULTS Regarding the overall job satisfaction, 50·2% nurses were dissatisfied and 40·4% nurses reported that they had intention to leave the current employment. Respondents' characteristics had an impact on job satisfaction and their intention to leave. The results showed that age, marital status, work experience, overall job satisfaction, job satisfaction: extrinsic rewards, interaction, praise/recognition and control/responsibility were significant factors contributing to nurses' intention to leave. CONCLUSIONS The finding may be a cause of concern for hospital management and highlights the importance of the two concepts (job satisfaction and intention to leave) in Shanghai. Innovative and adaptable managerial interventions need to be taken to improve nurses' job satisfaction and to strengthen their intention to stay. RELEVANCE TO CLINICAL PRACTICE Our findings outline some issues contributing to these problems and provide nurse administrators with information regarding specific influences on nurses' job satisfaction and intention to leave in Shanghai and innovative and adaptable managerial interventions that are needed. Our findings may also provide direction for nurse managers and healthcare management to implement strategies to improve nurses' job satisfaction and their intention to stay.
Optimal Spacing of “Covered” and “Exposed” Time Intervals in a Stochastic Process with High Penalty Costs: Applications to Parking and Insurance

This paper studies a class of policies for a stochastic process that is constituted of several time intervals of total time T. The intervals can be covered (or insured) at a pay-per-use rate or exposed (uninsured) with the risk of a large penalty. A decision maker has the three options: (i) Pay the user fee for the full period, (ii) not pay at all, and (iii) sporadically pay a user fee leaving an uncovered period at the end of each covered one. The penalty risk is assumed to occur during an uncovered interval according to a Poisson process. We present the expected cost model and find the optimal coverage policy. We present conditions under which it is always optimal to pay in full or not pay at all to minimize the expected total cost. Finally, we relax two assumptions and allow for the consideration of setup costs for every time the decision maker pays the coverage fees as well as a random duration T and derive new conditions for optimal strategies. Possible application of our model is paying parking meter fees and deciding between self-insuring one’s property and buying full (or partial) insurance coverage.
Cerebral oxygenation decreases but does not impair performance during self‐paced, strenuous exercise

Aim: The reduction in cerebral oxygenation (Cox) is associated with the cessation of exercise during constant work rate and incremental tests to exhaustion. Yet in exercises of this nature, ecological validity is limited due to work rate being either fully or partly dictated by the protocol, and it is unknown whether cerebral deoxygenation also occurs during self‐paced exercise. Here, we investigated the cerebral haemodynamics during a 5‐km running time trial in trained runners.
Gut microbial diversity is reduced and is associated with colonic inflammation in a piglet model of short bowel syndrome

Background and objectives: Following small bowel resection (SBR), the luminal environment is altered, which contributes to clinical manifestations of short bowel syndrome (SBS) including malabsorption, mucosal inflammation and bacterial overgrowth. However, the impact of SBR on the colon has not been well-defined. The aims of this study were to characterize the colonic microbiota following SBR and to assess the impact of SBR on mucosal inflammation in the colon. Results Analysis of the colonic microbiota demonstrated that there was a significant level of dysbiosis both two and six weeks post-SBR, particularly in the phylum Firmicutes, coupled with a decrease in overall bacterial diversity in the colon. This decrease in diversity was associated with an increase in colonic inflammation six weeks post-surgery. Methods Female (4-week old) piglets (5−6/group) received a 75% SBR, a transection (sham) or no surgery. Compositional analysis of the colonic microbiota was performed by high-throughput sequencing, two- and six-weeks post-surgery. The gene expression of the pro-inflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, IL-18 and tumor necrosis factor (TNF)-α in the colonic mucosa was assessed by qRT-PCR and the number of macrophages and percentage inducible nitric oxide synthase (iNOS) staining in the colonic epithelium were quantified by immunohistochemistry. Conclusions SBR significantly decreased the diversity of the colonic microbiota and this was associated with an increase in colonic mucosal inflammation. This study supports the hypothesis that SBR has a significant impact on the colon and that this may play an important role in defining clinical outcome.
Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., endemic yeasts associated with Protea flowers in South Africa.

In a taxonomic study of yeasts recovered from nectar of flowers and associated insects in South Africa, 11 strains were found to represent two novel species. Morphological and physiological characteristics and sequence analyses of the large-subunit rRNA gene D1/D2 region, as well as the actin, RNA polymerase II and elongation factor 2 genes, showed that the two novel species belonged to the genus Metschnikowia. Metschnikowia drakensbergensis sp. nov. (type strain EBD-CdVSA09-2(T) =CBS 13649(T) =NRRL Y-63721(T); MycoBank no. MB809688; allotype EBD-CdVSA10-2(A) =CBS13650(A) =NRRL Y-63720(A)) was recovered from nectar of Protea roupelliae and the beetle Heterochelus sp. This species belongs to the large-spored Metschnikowia clade and is closely related to Metschnikowia proteae, with which mating reactions and single-spored asci were observed. Metschnikowia caudata sp. nov. (type strain EBD-CdVSA08-1(T) =CBS 13651(T) =NRRL Y-63722(T); MycoBank no. MB809689; allotype EBD-CdVSA57-2(A) =CBS 13729(A) =NRRL Y-63723(A)) was isolated from nectar of Protea dracomontana, P. roupelliae and P. subvestita and a honeybee, and is a sister species to Candida hainanensis and Metschnikowia lopburiensis. Analyses of the four sequences demonstrated the existence of three separate phylotypes. Intraspecies matings led to the production of mature asci of unprecedented morphology, with a long, flexuous tail. A single ascospore was produced in all compatible crosses, regardless of sequence phylotype. The two species appear to be endemic to South Africa. The ecology and habitat specificity of these novel species are discussed in terms of host plant and insect host species.
Effects of gastric pacing on gastric emptying and plasma motilin.

AIM To investigate the effects of gastric pacing on gastric emptying and plasma motilin level in a canine model of gastric motility disorders and the correlation between gastric emptying and plasma motilin level. METHODS Ten healthy Mongrel dogs were divided into: experimental group of six dogs and control group of four dogs. A model of gastric motility disorders was established in the experimental group undergone truncal vagotomy combined with injection of glucagon. Gastric half-emptying time (GEt(1/2)) was monitored with single photon emission computerized tomography (SPECT), and the half-solid test meal was labeled with an isotope-(99m)Tc sulfur colloid. Plasma motilin concentration was measured with radioimmunoassay (RIA) kit. Surface gastric pacing at 1.1-1.2 times the intrinsic slow-wave frequency and a superimposed series of high frequency pulses (10-30 Hz) was performed for 45 min daily for a month in conscious dogs. RESULTS After surgery, GEt(1/2) in dogs undergone truncal vagotomy was increased significantly from 56.35+/-2.99 min to 79.42+/-1.91 min (P<0.001), but surface gastric pacing markedly accelerated gastric emptying and significantly decreased GEt(1/2) to 64.94+/-1.75 min (P<0.001) in animals undergone vagotomy. There was a significant increase of plasma level of motilin at the phase of IMCIII (interdigestive myoelectrical complex, IMCIII) in the dogs undergone bilateral truncal vagotomy (baseline vs vagotomy, 184.29+/-9.81 pg/ml vs 242.09+/-17.22 pg/ml; P<0.01). But plasma motilin concentration (212.55+/-11.20 pg/ml; P<0.02) was decreased significantly after a long-term treatment with gastric pacing. Before gastric pacing, GEt(1/2) and plasma motilin concentration of the dogs undergone vagotomy showed a positive correlation (r=0.867, P<0.01), but after a long-term gastric pacing, GEt(1/2) and motilin level showed a negative correlation (r=-0.733, P<0.04). CONCLUSION Surface gastric pacing with optimal pacing parameters can improve gastric emptying parameters and significantly accelerate gastric emptying and can resume or alter motor function in a canine model of motility disorders. Gastric emptying is correlated well with plasma motilin level before and after pacing, which suggests that motilin can modulate the mechanism of gastric pacing by altering gastric motility.
Wii Fit® training vs. Adapted Physical Activities: which one is the most appropriate to improve the balance of independent senior subjects? A randomized controlled study

Objective: To compare the effectiveness of three protocols (Adapted Physical Activities, Wii Fit®, Adapted Physical Activities + Wii Fit®) on the balance of independent senior subjects. Design: Case comparison study. Settings: Healthy elderly subjects living in independent community dwellings. Subjects: Thirty-six subjects, average age 75.09 ± 10.26 years, took part in this study, and were randomly assigned to one of the four experimental groups: G1 followed an Adapted Physical Activities training programme, while the second group (G2) participated in Wii Fit® training and the third one (G3) combined both methods. There was no training for the fourth group (G4). All subjects trained once a week (1 hour) for 20 weeks and were assessed before and after treatment. Main measures: The Tinetti test, unipedal tests and the Wii Fit® tests. Results: After training, the scores in the Tinetti test decreased significantly (P < 0.05) for G1, G2 and G3 respectively in static conditions and for G1 and G3 in dynamic conditions. After training, the performance in the unipedal tests decreased significantly (P < 0.05) for G1 and G3. The position of the centre of gravity was modified significantly (P < 0.05) for G2 and G3. Conclusion: After 20 training sessions, G1 (Adapted Physical Activities), G2 (Wii Fit®) and G3 (Adapted Physical Activities and Wii Fit®) improved their balance. In addition, G1 and G3 increased their dynamic balance. The findings suggest that Adapted Physical Activities training limits the decline in sensorial functions in the elderly.
What Do Programmers Know about Software Energy Consumption?

Traditionally, programmers received a range of training on programming languages and methodologies, but they rarely receive training on software energy consumption. Yet, the popularity of mobile devices and cloud computing requires increased awareness of software energy consumption. On mobile devices, battery life often limits computation. Under the demands of cloud computing, datacenters struggle to reduce energy consumption through virtualization and datacenter-infrastructure-management systems. Efficient software energy consumption is increasingly becoming an important nonfunctional requirement for programmers. However, are programmers knowledgeable enough about software energy consumption? Do they base their implementation decision on popular beliefs? Researchers surveyed more than 100 programmers regarding their knowledge of software energy consumption. They found that the programmers had limited knowledge of energy efficiency, lacked knowledge of the best practices to reduce software energy consumption, and were often unsure about how software consumes energy. These results highlight the need for better training and education on energy consumption and efficiency.
‘Who's who’ in renal sphaerosporids (Bivalvulida: Myxozoa) from common carp, Prussian carp and goldfish – molecular identification of cryptic species, blood stages and new members of Sphaerospora sensu stricto

SUMMARY Myxozoans are a group of diverse, spore-forming metazoan microparasites bound to aquatic environments. Sphaerospora dykovae (previously S. renicola) causes renal sphaerosporosis and acute swim bladder inflammation (SBI) in juvenile Cyprinus carpio carpio, in central Europe. A morphologically similar species with comparably low pathogenicity, S. angulata has been described from C. c. carpio, Carassius auratus auratus and Carassius gibelio. To clarify uncertainties and ambiguities in taxon identification in these hosts we decided to re-investigate differences in spore morphology using a statistical approach, in combination with SSU and LSU rDNA sequence analyses. We found that developing spores of S. angulata and S. dykovae cannot be distinguished morphologically and designed a duplex PCR assay for the cryptic species that demonstrated S. dykovae is specific to C. c. carpio, whereas S. angulata infects C. a. auratus and C. gibelio. The molecular identification of myxozoan blood stages in common carp and goldfish, which had previously been ascribed to Sphaerospora spp. showed that approximately 75% of blood stages were from non-sphaerosporid coelozoic species infecting these cyprinids and more than 10% were from an alien species, Myxobilatus gasterostei, developing in sticklebacks. We hereby report non-selective myxozoan host invasion and multi-species infections, whose role in SBI still requires clarification.
Polyamine-Mediated Effects of Prolactin Dictate Emergence from Mink Obligate Embryonic Diapause1

ABSTRACT Embryonic diapause is an evolutionary strategy to ensure that offspring are born when maternal and environmental conditions are optimal for survival. In many species of carnivores, obligate embryonic diapause occurs in every gestation. Reciprocal embryo transplant studies indicate that embryo arrest during diapause is conferred by uterine conditions and is due to a lack of specific factors necessary for continued development. In previous studies, global gene expression analysis revealed reduced uterine expression during diapause of a cluster of genes in the mink that regulate the abundance of polyamines, including ornithine decarboxylase 1 (ODC1). In addition, in vivo inhibition of the conversion of ornithine to the polyamine, putrescine, induced a reversible arrest in mink embryonic development and an arrest in trophoblast cell proliferation in vitro. Previous studies have implicated prolactin as the principal endocrine signal to terminate diapause. In this study, uterine expression of both the progesterone and estrogen receptors remained low at reactivation whilst the prolactin receptor was expressed at all times. Treatment of mink uterine epithelial cells with varying doses of prolactin indicated that this hormone induces ODC1 expression in the uterus via pSTAT1 and mTOR, thereby regulating uterine polyamine levels. In addition, we performed global gene expression analysis on mink embryos to further explore dynamic changes during diapause and found 94 genes upregulated at reactivation from diapause. Three polyamine-related genes, including ODC1, were also upregulated at reactivation from diapause. To establish whether polyamines mitigate escape from embryonic diapause, we collected mink embryos in diapause and incubated them in vitro with putrescine. Increase in embryo volume, the first indication of emergence from diapause, was observed within the first 5 days of culture in all viable embryos treated with putrescine, and the duration of embryo survival was increased threefold. Concomitant increases were also observed in both the total number of cells and the proportion of dividing cells in putrescine-treated embryos whilst control embryos remained in the diapause state. In further studies, inhibition of polyamine synthesis abrogated proliferation in cells derived from the inner cell mass of the mink embryo, while putrescine induced dose-dependent increases in cell division. We conclude that supplementation of embryos in diapause with putrescine results in their escape from developmental dormancy. These results provide strong evidence that obligate diapause in vivo is caused by the paucity of polyamines necessary for activation of the embryo after prolactin-induced termination of diapause.
Carteiras Igualmente Ponderadas com Poucas Ações e o Pequeno Investidor

This article analyzes equally-weighted stock portfolios (1/N) focusing on unsophisticated investors with small portfolios. The number of shares included in the 1/N portfolios ranged from 6 to 16 with rebalancing executed only three times a year. The period of analysis included daily and monthly returns between 1998 and 2011. The performance of the 1/N portfolios was compared to stock mutual funds, a global minimum variance portfolio with a 10% limit on positive weights (MVP 10%) and the Ibovespa index. The comparisons employed nonparametric tests, measures of risk-adjusted return and considered transaction costs. The results indicate that the performance of the selected funds is, at best, equivalent to the 1/N portfolios, albeit with a lower standard deviation. There was no significant difference in the median returns of the 1/N portfolios relative to the Ibovespa and MVP 10%. The stock selection criterion according to the ranking by the previous period Sharpe ratio is relevant, but rebalancing may be carried out less than three times a year. The 1/N portfolios are an attractive alternative to stock funds for investors with small stock portfolios even though their transaction costs may exceed 400 basis points per year.