Miracle-dz/UniFAR-FTUs huggingface dataset:
Acetylation and methylation profiles of H3K27 in porcine embryos cultured in vitro

Summary Methylation and acetylation of histone H3 at lysine 27 (H3K27) regulate chromatin structure and gene expression during early embryo development. While H3K27 acetylation (H3K27ac) is associated with active gene expression, H3K27 methylation (H3K27me) is linked to transcriptional repression. The aim of this study was to assess the profile of H3K27 acetylation and methylation (mono-, di- and trimethyl) during oocyte maturation and early development in vitro of porcine embryos. Oocytes/embryos were fixed at different developmental stages from germinal vesicle to day 8 blastocysts and submitted to an immunocytochemistry protocol to identify the presence and quantify the immunofluorescence intensity of H3K27ac, H3K27me1, H3K27me2 and H3K27me3. A strong fluorescent signal for H3K27ac was observed in all developmental stages. H3K27me1 and H3K27me2 were detected in oocytes, but the fluorescent signal decreased through the cleavage stages and rose again at the blastocyst stage. H3K27me3 was detected in oocytes, in only one pronucleus in zygotes, cleaved-stage embryos and blastocysts. The nuclear fluorescence signal for H3K27me3 increased from the 2-cell stage to 4-cell stage embryos, decreased at the 8-cell and morula stages and increased again in blastocysts. Different patterns of the H3K27me3 mark were observed at the blastocyst stage. Our results suggest that changes in the H3K27 methylation status regulate early porcine embryo development as previously shown in other species.
Sedative and cardiorespiratory effects of detomidine constant rate infusion in sheep

The use of sheep in experiments is widespread and is increasing worldwide, and so is the need to develop species-specific anaesthetic techniques to ensure animal safety. Previous studies have mentioned several protocols involving the administration of alpha-2 adrenergic agonists in sheep; however, assessment of the efficacy and safety of these infusion techniques is still relatively new. Thus, the aim of the present study is to assess the effectiveness of detomidine constant rate infusion (CRI) in sheep by measuring the cardiovascular and respiratory parameters, blood gas variables and sedation scores. Eight adult female Santa Inês sheep received 20 µg/kg of detomidine hydrochloride intravenously as a bolus loading dose, followed by an infusion rate of 60 µg/kg/h. The heart rates and respiratory rates changed continuously during the CRI period. No arrhythmias were observed. The reduction in arterial partial pressure of oxygen (PaO2) was not significant, but one animal showed signs of hypoxaemia (minimum PaO2 of 66.9 mmHg). The arterial partial pressure of carbon dioxide (PaCO2) increased, but the animals did not become hypercapnic. The bicarbonate (HCO3−), pH and base excess (BE) tended towards metabolic alkalosis. The cardiac output (CO), stroke volume (SV), cardiac index (CI) and ejection fraction (EF%) showed no significant changes. The fractional shortening (FS%) decreased slightly, starting at T45min. Sedation scores varied between 3 (0/10) after sedation and during recovery and 7 (0/10) during CRI. We concluded that administering detomidine at an infusion rate of 60 µg/kg/h in Santa Inês sheep is a simple technique that produces satisfactory sedation for minimally invasive procedures.
An Aboriginal Australian Genome Reveals Separate Human Dispersals into Asia

Whole-genome data indicate that early modern humans expanded into Australia 62,000 to 75,000 years ago. We present an Aboriginal Australian genomic sequence obtained from a 100-year-old lock of hair donated by an Aboriginal man from southern Western Australia in the early 20th century. We detect no evidence of European admixture and estimate contamination levels to be below 0.5%. We show that Aboriginal Australians are descendants of an early human dispersal into eastern Asia, possibly 62,000 to 75,000 years ago. This dispersal is separate from the one that gave rise to modern Asians 25,000 to 38,000 years ago. We also find evidence of gene flow between populations of the two dispersal waves prior to the divergence of Native Americans from modern Asian ancestors. Our findings support the hypothesis that present-day Aboriginal Australians descend from the earliest humans to occupy Australia, likely representing one of the oldest continuous populations outside Africa.
Domestic Spillovers and Foreign Networks in Exporting

We explore the effects of Domestic Spillovers (other domestic firms exporting a good to a given country) and Foreign Networks (links to other countries via firm’s trading partners) on the firm’s choice of new export destinations. By matching Colombian exporting and Chilean importing and exporting firms between 2007 and 2016, we show that both effects matter and that they amplify each other. Jointly these effects are more important than geographic proximity measures, import and export growth, and market size combined. Domestic spillovers are relatively more important, especially for identifying the persistent exporters, which export to a new destination for more than a year.
Quantum jumps in atomic systems.

We present a microscopic model for the so-called quantum jumps in three-level atoms with one transition between two levels being strongly driven and the third level being metastable. Our model is based on calculating the elementary probability density P(0,t)(t1, … , t n ) which gives the probability density that exactly n photons are emitted at times t1, … , t n by the atom in the time interval (0, t) on the strong transition. This enables us to calculate the conditional probabilities c ˜ ( τ ) that given a photon has emitted at τ = 0 the next photon emission occurs at time τ. This quantity is shown to be the sum of two exponentials which for sufficient differetial in the time scales describe the periods of light and darkness. Using this conditional probability it is possible to numerically simulate the individual photon emissions.
[The presence of Mycoplasma hominis and Ureaplasma urealyticum in the cervical canal of uterus].

OBJECTIVES Mycoplasma hominis and Ureaplasma species are common commensal inhabitants of the lower genitourinary tract in adolescents and adults who are sexually active. A lot of authors points out that these microorganisms can play an important role in pathology of genital tract like pelvic inflammatory disease, sterility or non-gonococcal urethritis. MATERIALS AND METHODS In the study samples from cervical canal of the uterus were obtained from 222 women. The first group consist of 132 women who were examined in II Gynecological Clinic in Wroclaw for different, probably infectious, gynecological pathologies (adnexitis, sterility, bacterial vaginosis). 90 women without infectious diseases were in a control group. All swabs taken from cervix were tested for Mycoplasma hominis and Ureaplasma urealyticum. RESULTS The prevalence of Ureaplasma urealyticum was 31.8% in the first tested group (42 of 132 women were positive) and 8.8% in control group (8 of 90 were positive). 3% (4 of 132) of patients were positive to Mycoplasma hominis in the first group and only 1.1% (1 of 90) in a control group. CONCLUSIONS Ureaplasma urealyticum was found most often in such genital tract pathologies like acute or recurrent adnexitis, sterility or bacterial vaginosis. No statistically significant correlation was found between the age of the patients and the incidence of mycoplasmas.
When is brand content shared on Facebook? A field study on online Word-of-Mouth

When implementing social media strategies on Facebook brand pages, companies focus on factors that prompt electronic Word-of-Mouth (eWoM) about brand contents. This study examines individual and collective factors that lead to brand content sharing behaviors. The research conducts a field study of a real brand on its Facebook fan page to gather actual behavioral data. Both declarative and behavioral data are used to explain brand eWoM, based on 250 participants. Empirical results show that the Facebook activity has a positive influence on brand content sharing for active users (posters) but not for nonactive users (lurkers), while brand engagement positively affects brand content sharing for both active and nonactive users. Furthermore, brand community involvement does not have an influence on brand content sharing. Findings offer companies useful recommendations for using the determinants included in our research design to understand and analyze the sharing behaviors of users.
Primed CD8+ T‐Cell Responses to Allogeneic Endothelial Cells Are Controlled by Local Complement Activation

CD8 T cells primed by transplantation recognize allogeneic class I MHC molecules expressed on graft vascular endothelium and contribute to allograft injury. We previously showed that immune cell‐derived complement activation fragments are integral to T cell activation/expansion. Herein we tested the impact of local complement production/activation on T cell/endothelial cell (EC) interactions. We found that proinflammatory cytokines upregulated alternative pathway complement production by ECs, yielding C5a. We further found that ECs deficient in the cell surface C3/C5 convertase regulator decay accelerating factor (DAF, CD55) induced greater CD8 T‐cell proliferation and more IFNγ+ and perforin+ effector cells than wild‐type (WT) ECs. Allogeneic C3−/− EC induced little or no CD8 responses. Abrogation of responses following C5a receptor (C5aR) blockade, or augmentation following addition of recombinant C5a demonstrated that the effects were mediated through T‐cell‐expressed‐C5aR interactions. Analyses of in vivo CD8 cell responses to transplanted heart grafts deficient in EC DAF showed similar augmentation. The findings reveal that EC‐derived complement triggers secondary CD8 T‐cell differentiation and expansion and argue that targeting complement and/or C5aR could limit T‐cell‐mediated graft injury.
Self-Improving Motivations and Collectivism

Much research reveals pronounced self-improving motivations among East Asians. A question remains whether similar patterns would emerge in non-Asian collectivist cultures. Research that has used self-report measures reveals mixed evidence regarding collectivist self-improvement. The present study assesses self-improving motivations among Chileans using a behavioral measure in a replication of an earlier experiment with Americans and Japanese by Heine and colleagues (2001). The results revealed that Chileans, like Japanese and unlike Americans, were more likely to switch tasks following success feedback than following failure feedback.
A functional MRI study of subjects recovered from hemiparetic stroke.

BACKGROUND AND PURPOSE Stroke recovery mechanisms remain incompletely understood, particularly for subjects with cortical stroke, in whom limited data are available. We used functional magnetic resonance imaging to compare brain activations in normal controls and subjects who recovered from hemiparetic stroke. METHODS Functional magnetic resonance imaging was performed in ten stroke subjects with good recovery, five with deep, and five with cortical infarcts. Brain activation was achieved by index finger-tapping. Statistical parametric activation maps were obtained using a t test and a threshold of P < . 001. In five bilateral motor regions, the volume of activated brain for each stroke subject was compared with the distribution of activation volumes among nine controls. RESULTS Control subjects activated several motor regions. During recovered hand finger-tapping, stroke subjects activated the same regions as controls, often in a larger brain volume. In the unaffected hemisphere, sensorimotor cortex activation was increased in six of nine stroke subjects compared with controls. Cerebellar hemisphere contralateral and premotor cortex ipsilateral to this region, as well as supplementary motor areas, also had increased activation. In the stroke hemisphere, activation exceeding controls was uncommon, except that three of five cortical strokes showed peri-infarct activation foci. During unaffected hand finger-tapping, increased activation by stroke subjects compared with controls was uncommon; however, decreased activation was seen in unaffected sensorimotor cortex, suggesting that this region's responsiveness increased to the ipsilateral hand and decreased to contralateral hand movements. Use of a different threshold for defining activation (P < .01) did not change the overall findings (kappa = .75). CONCLUSIONS Recovered finger-tapping by stroke subjects activated the same motor regions as controls but to a larger extent, particularly in the unaffected hemisphere. Increased reliance on these motor areas may represent an important component of motor recovery. Functional magnetic resonance imaging studies of subjects who recovered from stroke provide evidence for several processes that may be related to restoration of neurologic function.
Familial lymphocytic thyroiditis: analysis of linkage with histocompatibility and blood groups.

ABSTRACT An association between Hashimoto's thyroiditis, the major histocompatibility loci and certain blood antigens has been sought. The diagnosis of thyroiditis was firmly established in 13 members of 4 families and in an additional 7 there was suggestive evidence of the disorder. Our investigation of HL-A antigens in these patients and other family members failed to reveal any consistent association with thyroiditis. There was also no linkage demonstrated between thyroiditis and blood or serum group antigens. Although linkage has been described between histocompatibility antigens and experimental thyroiditis in the mouse, such a relationship is lacking in Hashimoto's thyroiditis.
Pharmacokinetics, Bioavailability, Tissue Distribution, Excretion, and Metabolite Identification of Methoxyflavones in Kaempferia parviflora Extract in Rats

Kaempferia parviflora (KP) is an herbal plant in the family of Zingiberaceae. KP mainly contains methoxyflavones, especially 5,7-dimethoxyflavone (DMF), 5,7,4′-trimethoxyflavone (TMF), and 3,5,7,3′,4′-pentamethoxyflavone (PMF). The present study was designed to characterize the pharmacokinetics, including bioavailability, distribution, excretion, and identification of metabolites after administration of a KP ethanolic extract. Male rats were orally or intravenously administered a 250 mg/kg concentration of a KP extract, and blood samples were obtained at selected times to determine pharmacokinetic parameters of PMF, TMF, and DMF. For distribution and excretion studies, the organs, urine, and feces samples were collected at various times after oral administration of a larger (750 mg/kg) dose of KP extract. Methoxyflavones in the biological samples were quantified by high-performance liquid chromatography-UV, and the metabolites in urine and feces were further identified by using liquid chromatography-tandem mass spectrometry. After oral administration, concentrations of the three methoxyflavones quickly approached their maximal concentration, ranging from 0.55 to 0.88 μg/ml within 1 to 2 h after administration, and then were gradually excreted with half-lives of 3 to 6 h. The methoxyflavones showed low oral bioavailability of 1 to 4%. Three methoxyflavones were detected at their highest levels in liver followed by kidney. They were also found in lung, testes, and brain. After absorption, organ distribution, and metabolism, the components of KP were mainly eliminated through urine in the forms of demethylated, sulfated, and glucuronidated products and as demethylated metabolites in the feces. The parent compounds were found to have 0.79, 1.76, and 3.10% dose recovery in urine and 1.06, 1.77, and 0.96% dose recovery in feces for PMF, TMF, and DMF, respectively. These studies are the first to describe the pharmacokinetics of KP extract to provide the information on blood and tissue levels.
Emergence of Regional Entrepreneurship Through Hometown Tax Donation - Unique Regional Development System: Implications from Development of New Products and Improvement in Business Capabilities by Gift Providers -

Hometown Tax Donation is designed to bolster regional economies through the provision of reciprocal gifts by local businesses, and can be regarded as a unique SME policy which is not reliant on subsidies, but instead, offers businesses the incentive to develop attractive products and yields better results when businesses and municipalities collaborate closely. To assess its effectiveness, we surveyed certain gift providers on their efforts in new product development and advancement in business capabilities. Results showed that the gift market is sowing the seeds for development of new products and businesses in rural areas, and is also boosting business capabilities in many cases. Meanwhile, certain companies have earned large revenues from gifts or are overdependent on gifts. It is therefore necessary to make Hometown Tax Donation function as a viable SME policy which incorporates an exit strategy.
Understanding the adoption of business intelligence in decision-making within the IT distribution industry

Demand visibility in the distribution supply chain is obscured for IT distributors because there is limited sight of the end-consumer. Therefore, it is important that distributors collect and analyse reseller data to obtain valuable insights. This assists in adding value to the supply chain. However, few South African distributors have adopted business intelligence (BI) which enables this analysis. Consequently, the objective of the study was to understand how senior management used BI to drive evidence-based decisions. The exploratory study employed qualitative methods to explore the adoption of BI among senior management in the South African distribution industry. A predominantly inductive approach was used to interpret the interview results. These insights created the basis on which the conceptual model of evidence-based decision-making was built. The findings were that critical success factors of BI adoption supported and extended the existing literature. Though the consistent adoption of BI was lacking, evidence was found in support of the information processing capability of BI. The presence of senior management’s absorptive capacity played a direct role in the successful adoption of BI. These provided further evidence that datadriven decisions required a combination of management’s ability to synthesise data and capitalise on the opportunities presented in the data. Data, used effectively, allowed for improved demand visibility within the distribution channel.
Evidence for mucins and sialic acid as receptors for Pseudomonas aeruginosa in the lower respiratory tract

The nature of the receptors for mucoid and nonmucoid Pseudomonas aeruginosa was investigated by using adherence to injured tracheal epithelium as a model. Bovine submaxillary mucin and crude rat tracheal mucin inhibited the adherence of both types of P. aeruginosa. Among the sugars present in these mucins only N-aceylneuraminic acid inhibited adherence. Inhibition of adherence probably involved the binding of N-acetylneuraminic acid to the bacterial cells and not to the tracheal cells. The mucoid strain appeared to be much more sensitive to inhibition by N-acetylneuraminic acid. Periodate oxidation and cholera filtrate also reduced the adherence of both strains, but Clostridium perfringens neuraminidase treatment did not alter adherence. A nonmucoid isogenic mutant of an unstable mucoid strain was also inhibited by N-acetylneuraminic acid. These data suggest that the receptor for P. aeruginosa is a sialic acid moiety on cell surfaces or in mucins.
Association of hand and arm disinfection with asthma control in US nurses

Objectives To investigate the association between occupational exposure to disinfectants/antiseptics used for hand hygiene and asthma control in nurses. Methods In 2014, we invited female nurses with asthma drawn from the Nurses’ Health Study II to complete two supplemental questionnaires on their occupation and asthma (cross-sectional study, response rate: 80%). Among 4055 nurses (mean age: 59 years) with physician-diagnosed asthma and asthma medication use in the past year, we examined asthma control, as defined by the Asthma Control Test (ACT). Nurses were asked about the daily frequency of hand hygiene tasks: ‘wash/scrub hands with disinfectants/hand sanitizers’ (hand hygiene) and ‘wash/scrub arms with disinfecting products’ (surrogate of surgical hand/arm antisepsis). Analyses were adjusted for age, race, ethnicity, smoking status and body mass index. Results Nurses with partly controlled asthma (ACT: 20–24, 50%) and poorly controlled asthma (ACT ≤19, 18%) were compared with nurses with controlled asthma (ACT=25, 32%). In separate models, both hand and arm hygiene were associated with poorly controlled asthma. After mutual adjustment, only arm hygiene was associated with poorly controlled asthma: OR (95% CI) for <1 time/day, 1.38 (1.06 to 1.80); ≥1 time/day, 1.96 (1.52 to 2.51), versus never. We observed a consistent dose–response relationship between frequency of arm hygiene tasks (never to >10 times/day) and poor asthma control. Associations persisted after further adjustment for surfaces/instruments disinfection tasks. Conclusions Frequency of hand/arm hygiene tasks in nurses was associated with poor asthma control. The results suggest an adverse effect of products used for surgical hand/arm antisepsis. This potential new occupational risk factor for asthma warrants further study.
Applying the Institutional Theory at the Level of Halal Consumers: The Case of Cape Town in South Africa

ABSTRACT The institutional theory, especially at an individual level, has not been conducted on halal consumers in South Africa. The purpose of this study was to evaluate the degree to which halal consumers who have higher institutional pressures are more expected to purchase halal food products. This study draws upon institutional theory to present empirical evidence that institutional factors may show a significant effect on halal consumer’s purchase intention and buying behavior toward purchasing halal food products. A sample of 298 halal consumers in South Africa was selected. Data samples were collected via self-administrated questionnaires. Structural equation modeling (SEM) was used to test study hypotheses. The study showed that all institutional factors have a direct effect on halal consumer’s intention and indirect effect on buying behavior. Based on the results, normative pressures had a high significant effect among institutional pressures, followed by other factors such as mimetic and coercive pressures, respectively. This study is first of the uncommon studies examining halal consumers’ purchase intention and buying behavior in a non-Muslim country, employing the institutional theory in the context of halal food consumption.
Clustering Kuwaiti consumer attitudes towards Sharia-compliant financial products

PurposeThis purpose of this paper is to clustering Kuwaiti consumers choice of Sharia-complaint financial products and services based on several factors such as religiosity, financial knowledge, customer value, satisfaction, trust, service quality, relationship with service providers and innovation.Design/methodology/approachThe study uses self-organizing maps, a neural network technique, in order to cluster and segment consumers of Sharia-compliant financial products and services in Kuwait. From a marketing perspective, SOM can be viewed as a flexible clustering technique in which different clusters are identified without the rigid traditional statistical assumptions of linearity or normality.FindingsThis paper shows that consumers of Sharia-compliant financial products in Kuwait can be clustered into three distinct segments: enthusiasts, laggards and rejectors. The enthusiasts represent the largest cluster with a frequency of around 66 percent, while the Rejectors represent the smallest segment with a frequency of 10 percent.Originality/valueThis paper advances our knowledge about the behavioral aspects of financial consumer choice within a non-traditional Sharia-compliant financial products context.