Miracle-dz/UniFAR-FTUs huggingface dataset:
On the Minimum Number of k-Cliques in Graphs with Restricted Independence Number

We investigate the following problem of Erdős: Determine the minimum number of k-cliques in a graph of order n with independence number [ges ] l. We disprove the original conjecture of Erdős which was stated in 1962 for all but a finite number of pairs k, l, and give asymptotic estimates for l = 3, k [ges ] 4, and l = 4, k [ges ] 4.
Spondylo‐epiphyseal dysplasia, Maroteaux type (pseudo‐Morquio syndrome type 2), and parastremmatic dysplasia are caused by TRPV4 mutations

Recent discoveries have established the existence of a family of skeletal dysplasias caused by dominant mutations in TRPV4. This family comprises, in order of increasing severity, dominant brachyolmia, spondylo‐metaphyseal dysplasia Kozlowski type, and metatropic dysplasia. We tested the hypothesis that a further condition, Spondylo‐epiphyseal dysplasia (SED), Maroteaux type (MIM 184095; also known as pseudo‐Morquio syndrome type 2), could be caused by TRPV4 mutations. We analyzed six individuals with Maroteaux type SED, including three who had previously been reported. All six patients were found to have heterozygous TRPV4 mutations; three patients had unreported mutations, while three patients had mutations previously described in association with metatropic dysplasia. In addition, we tested one individual with a distinct rare disorder, parastremmatic dysplasia (MIM 168400). This patient had a common, recurrent mutation seen in several patients with Kozlowski type spondylo‐metaphyseal dysplasia. We conclude that SED Maroteaux type and parastremmatic dysplasia are part of the TRPV4 dysplasia family and that TRPV4 mutations show considerable variability in phenotypic expression resulting in distinct clinical‐radiographic phenotypes. © 2010 Wiley‐Liss, Inc.
Contrasting responses of human gingival and periodontal ligament fibroblasts to bacterial cell-surface components through the CD14/Toll-like receptor system.

We compared human periodontal ligament fibroblasts with human gingival fibroblasts isolated from the same donor to examine interleukin-8 (IL-8) responses of the cells to Salmonella lipopolysaccharide, a water-soluble peptidoglycan from Staphylococcus epidermidis and the synthetic muramyldipeptide, with special reference to the possible involvement of the CD14/Toll-like receptor (TLR) system of the cells in the responses. Human gingival fibroblasts expressed CD14 on their surfaces and strongly expressed CD14 mRNA, while human periodontal ligament fibroblasts showed considerably lower levels of expression in both respects. Both cells expressed mRNA of TLR-related molecules, i.e. TLR2, TLR4, MD-2 and MyD88, although human periodontal ligament fibroblasts expressed TLR2 more strongly than human gingival fibroblasts. Human gingival fibroblasts exhibited a stronger IL-8 response than human periodontal ligament fibroblasts to lipopolysaccharide, while human periodontal ligament fibroblasts exhibited a response comparable to, or slightly stronger than, that of human gingival fibroblasts to S. epidermidis peptidoglycan and muramyldipeptide. The IL-8 responses of both cells to lipopolysaccharide and S. epidermidis peptidoglycan were completely inhibited by antihuman CD14 monoclonal antibody (MAb). The responses of both cells to lipopolysaccaride were significantly inhibited by antihuman TLR4 MAb, while those to S. epidermidis peptidoglycan were inhibited by antihuman TLR2 MAb. In contrast, muramyldipeptide activated both types of cells in a TLR2- and TLR4-independent manner, although the activities of muramyldipeptide on human gingival fibroblasts, but not human periodontal ligament fibroblasts, were significantly inhibited by anti-CD14 MAb.
Human Cytomegalovirus oriLyt Sequence Requirements

ABSTRACT The mechanisms of action and regulation of the human cytomegalovirus (HCMV) lytic-phase DNA replicator, oriLyt, which spans more than 2 kbp in a structurally complex region near the middle of the unique long region (UL), are not understood. Because oriLyt is thought to be essential for promoting initiation of lytic DNA synthesis and may participate in regulating the switch between lytic and latent phases, we undertook a mutational study to better define its sequence requirements. Kanr gene cassette insertions located an oriLyt core region between nucleotides (nt) 91751 and 93299 that is necessary but not sufficient for replicator activity in transient assays. In contrast, insertions into auxiliary regions flanking either side of this core—also required for significant replicator activity—had little effect. To search for essential components within the core region, we made a series of overlapping, roughly 200-bp deletions, and qualitatively and quantitatively assessed the abilities of the resulting constructs to mediate replication. All but one of these deletions produced a significant (i.e., greater than twofold) loss of activity, arguing that sequences across this entire region contribute to replicator function. However, two particularly critical segments separated by a dispensable region, here called essential regions I and II, were identified. Within essential region I, which overlaps the previously identified early transcript SRT, two adjacent but nonoverlapping, roughly 200-bp deletions abolished detectable replication. No single element or motif from the left half of essential region I was found to be essential. Thus, essential region I probably promotes replication through the cooperation of multiple elements. However, four small deletions in the right half of essential region I, which included or lay adjacent to the conserved 31-nt oligopyrimidine tract (referred to as the Y block), abolished or virtually abolished oriLyt activity. Together, these results identify candidate oriLyt sequences within which molecular interactions essential for initiation oforiLyt-mediated DNA synthesis are likely to occur.
Recombinant Human Platelet-Activating Factor- Acetylhydrolase Inhibits Airway Inflammation and Hyperreactivity in Mouse Asthma Model1

Numerous in vitro and in vivo studies in both animal models and human asthmatics have implicated platelet-activating factor (PAF) as an important inflammatory mediator in asthma. In a murine asthma model, we examined the anti-inflammatory activities of recombinant human PAF-acetylhydrolase (rPAF-AH), which converts PAF to biologically inactive lyso-PAF. In this model, mice sensitized to OVA by i.p. and intranasal (i.n.) routes are challenged with the allergen by i.n. administration. The OVA challenge elicits an eosinophil infiltration into the lungs with widespread mucus occlusion of the airways and results in bronchial hyperreactivity. The administration of rPAF-AH had a marked effect on late-phase pulmonary inflammation, which included a significant reduction in airway eosinophil infiltration, mucus hypersecretion, and airway hyperreactivity in response to methacholine challenge. These studies demonstrate that elevating plasma levels of PAF-AH through the administration of rPAF-AH is effective in blocking the late-phase pulmonary inflammation that occurs in this murine allergen-challenge asthma model. These results suggest that rPAF-AH may have therapeutic effects in patients with allergic airway inflammation.
F0 timing in Kinyarwanda.

In Kinyarwanda (Bantu, Rwanda), a high tone can occur on either the first or the second mora of a syllable. A study of f0 timing in the language was undertaken to determine how such a contrast is realized phonetically, and how this realization is affected by phrase position, word position, and distance from a following high tone. The study also aimed to explore a reported effect of 'tone anticipation' (raising of pitch in a syllable before a high tone) in the language. The study found significant effects of the position of a tone within the syllable, its position within the phrase, and its position with respect to following high tones. Models of f0 timing in Kinyarwanda are proposed, demonstrating the role of moras in phonetic implementation of tone. These models clarify issues regarding the phonological representation of tones in Kinyarwanda, and the division between phonetic patterns and phonological ones.
Pulsed-field gel electrophoresis typing of Campylobacter fetus subsp. fetus isolated from sheep abortions in New Zealand

Abstract AIMS: To genotype Campylobacter fetus subsp. fetus isolates cultured from sheep abortions submitted to diagnostic laboratories in New Zealand during the year 2000 breeding season. To compare the types found nationally with those found in the Hawke' Bay region in 1999, and strains held in the New Zealand Reference Culture Collection, Medical Section (NZRM) from a study published in 1987. METHODS: Campylobacter fetus subsp. fetus isolates cultured by veterinary diagnostic laboratories in the year 2000 breeding season, from sheep abortions from throughout New Zealand, were typed using pulsed-field gel electrophoresis (PFGE). In addition, seven freeze-dried C. fetus subsp. fetus isolates (strain numbers 2939–2945) from the NZRM, representing restriction types a–g found amongst sheep abortion isolates in a study published in 1987, were typed using PFGE. RESULTS: In total, 293 C. fetus subsp. fetus isolates from 200 farms were obtained from veterinary diagnostic laboratories. Twenty-two distinct PFGE profiles were identified amongst the isolates. PFGE type B1 was predominant in each region of New Zealand and was identified from 66% of farms overall. Of the C. fetus subsp. fetus restriction types a–g lodged with the NZRM, 3/7 had PFGE profiles indistinguishable from profiles found in the current study. The other four restriction types had PFGE profiles that were unique but similar to those found in the current study. CONCLUSIONS: PFGE type B1 was predominant amongst the C. fetus subsp. fetus isolates cultured from sheep abortions in each region of New Zealand in the year 2000, as was found in Hawke' Bay in 1999. The similarity between PFGE profiles of C. fetus subsp. fetus sheep abortion isolates from 1987 and 2000, and the relative prevalence of the PFGE groups, suggests that there has been no major genotypic shift in the population of C. fetus subsp. fetus implicated in sheep abortion in New Zealand during this time.
Dental Caries and Microbial Acid Production in Twins

Objective: To determine the relative contribution of genetic and environmental stimuli on dental caries traits and microbial acid production in a twin model. Methods: Dental caries examinations and microbial acid production assays were performed on 388 pairs of twins 1.5–8 years old from the city of Montes Claros, Brazil. Genotyping 8 polymorphic DNA markers determined zygosity. Caries exams followed NIDCR criteria modified to distinguish white spot lesions from cavitated lesions. Surface-based caries prevalence rates (SBCPR) were computed and lesion severity was determined by a weighted index (LSI). Biofilm samples were collected from the tongue using a lactic acid indicator swab. Assay scores were categorized based on acid formation as 1 = low, 2 = medium, and 3 = high. Heritability analyses were performed using the SOLAR software package. Results: Heritability estimates for SBCPRs, LSI and for microbial acid production were H = 76.3 (p < 0.001), H = 70.6 (p < 0.001), H = 16.2 (p = 0.0078), respectively. Treating microbial acid production as a covariate in the SBCPR and LSI models did not significantly alter the heritability estimates, i.e. H = 76.5 (p < 0.001) and H = 70.8 (p < 0.001), respectively. Conclusions: These results suggest that variation in dental caries surface traits has a significant genetic contribution and that microbial acid production is modulated by the environment.
Large scale simulations of solar type III radio bursts: flux density, drift rate, duration and bandwidth

Non-thermal electrons accelerated in the solar corona can produce intense coherent radio emission, known as solar type III radio bursts. This intense radio emission is often observed from hundreds of MHz in the corona down to the tens of kHz range in interplanetary space. It involves a chain of physical processes from the generation of Langmuir waves to nonlinear processes of wave-wave interaction. We develop a self-consistent model to calculate radio emission from a non-thermal electron population over large frequency range, including the effects of electron transport, Langmuir wave-electron interaction, the evolution of Langmuir waves due to non-linear wave-wave interactions, Langmuir wave conversion into electromagnetic emission, and finally escape of the electromagnetic waves. For the first time we simulate escaping radio emission over a broad frequency range from 500~MHz down to a few MHz and infer key properties of the radio emission observed: the onset (starting) frequency, {identification as fundamental or harmonic emission}, peak flux density, instantaneous frequency bandwidth, and timescales for rise and decay. Comparing with the observations, these large scale simulations enable us to identify the processes governing the key type III solar radio burst characteristics.
General practice audit of asthma in childhood

It has been suggested that asthma is underdiagnosed in children. We studied the records of 52 children with asthma in our group practice. Although asthma had been positively diagnosed in roughly 10% of the children aged 18 months to 11 years, to make the diagnosis required an average of 16 to 20 consultations for respiratory problems. According to the symptoms recorded before the diagnosis was made, only three quarters of the children had presented with wheeze and roughly a third had had difficulty in sleeping. We think that the doctor's time would be better spent teaching patients about their asthma than by repeatedly treating non-existent respiratory infection.
Peroxisome proliferator-activated receptor-&ggr; interrupts angiogenic signal transduction by transrepression of platelet-derived growth factor-&bgr; receptor in hepatic stellate cells

ABSTRACT Hepatic stellate cells (HSCs) are liver-specific pericytes that are recruited to vessels and secret pro-angiogenic cytokines, and thus actively involved in pathological vascularization during liver fibrosis. Peroxisome proliferator-activated receptor-&ggr; (PPAR&ggr;) is a switch molecule controlling HSC activation. We investigated PPAR&ggr; regulation of angiogenic signal transduction and the molecular mechanisms involved in HSCs. Primary rat HSCs and liver sinusoidal endothelial cells (LSECs) were isolated and used in this study. Boyden chamber and tubulogenesis assays, identified that focal adhesion kinase (FAK)–RhoA signaling activated by platelet-derived growth factor (PDGF) was required for HSC motility and the associated vascularization. PDGF also stimulated vascular endothelial growth factor (VEGF) expression and HSC-driven vascularization through signals mediated by extracellular signal-regulated kinase (ERK) and mammalian target of rapamycin (mTOR). Gain- and loss-of-function analyses demonstrated that activation of PPAR&ggr; interrupted FAK–RhoA, ERK and mTOR cascades and inhibited HSC-based vascularization. Molecular evidence further revealed that PPAR&ggr; attenuation of HSC angiogenic properties was dependent on inhibition of PDGF-&bgr; receptor expression. We concluded that PPAR&ggr; inhibited angiogenic signal transduction through transrepression of PDGF-&bgr; receptor leading to reduced HSC motility, reduced VEGF expression, and thereby attenuated HSC-driven angiogenesis. PPAR&ggr; could be a molecular target for preventing vascular remolding in hepatic fibrosis.
From Antecedent Conditions to Violent Actions: A General Affective Aggression Model

The General Affective Aggression Model (GAAM) posits that variables that increase aggression do so by increasing aggressive affect, aggressive cognition, or arousal. The effects of trait hostility, pain, and cognitive cues on state hostility (Experiment 1), on lexical decisions for aggressive and control words (Experiment 2), on escape motives (Experiment 3), and on aggressive behavior (Experiment 4) are presented. Consistent with GAAM, trait hostility increased both flight and fight motives, presumably due to affective reactions. Pain also increased hostile affect but increased aggression only when aggressive thoughts were made highly accessible (i.e., after viewing gun pictures). Theoretical implications are discussed.
Model-Driven Domain Analysis and Software Development - Architectures and Functions

Software developers use different techniques for identification and specification of a domain's characteristics and requirements for a planned application. The importance of this step cannot be understated as it is impossible to be highly efficient with a weak beginning, even with a strong end of the software development life cycle. Model-Driven Domain Analysis and Software Development: Architectures and Functions displays how to effectively map and respond to the real-world challenges and purposes which software must solve. The implications can be far-reaching and apply to domains such as mechatronic, embedded and high risk systems, where failure could cost human lives. It is also important for complex business systems, wherein failures could lead to huge financial losses. This book forms an essential reference for developers and researchers by providing both cases and theories to ensure a strong and suitable domain analysis to support all other efforts when creating and applying software solutions.
Overeating and Obesity from Damage to a Noradrenergic System in the Brain

A discrete, ascending fiber system that supplies the hypothalamus with most of its noradrenergic terminals was destroyed at midbrain level, both electrolytically and with local injections of 6-hydroxydopamine, a destructive agent specific for catecholaminergic neurons. The result was hyperphagia leading to obesity. Fluorescence histochemical analysis showed that loss of noradrenergic terminals in ventral bundle termination areas such as the hypothalamus was necessary for hyperphagia. Damage to dorsal bundle or dopaminergic projections was not. Prior treatment with desmethylimipramine to selectively block uptake of 6-hydroxydopamine into noradrenergic neurons prevented both hyperphagia and loss of norepinephrine fluorescence. The lesions that produced hyperphagia also reduced the potency of d-amphetamine as an appetite suppressant. It is concluded that this noradrenergic bundle normally mediates suppression of feeding, thereby influences body weight, and serves as a substrate for d-amphetamine-induced loss of appetite.
MEGA ♪ —Empirical Support for Nomenclature on the Anomalies

Applied are empirical findings supporting the authors’ previously presented nomenclature identifying two subsets of sexually abusive youth overlooked by most contemporary risk assessment tools: sexually violent and predatory sexually violent youth. The cross-validation findings on an ecologically framed risk assessment tool, MEGA ♪ (Multiplex Empirically Guided Inventory of Ecological Aggregates for Assessing Sexually Abusive Children and Adolescents [Ages 19 and Under]) (N = 1,056 male and female sexually abusive youth, ages 4-19, including youth with low intellectual functioning), from the United States, Canada, England, and Scotland, were utilized. Findings provided normative data, with cutoff scores according to age and gender. Most contemporary risk assessment tools have three levels (low, moderate, and high), which may in fact be limited in assessing the range of risk level. The MEGA ♪ cross-validation established a new range of risk level, with the fourth level (very high) definitively identifying the most dangerous youth, thus empirically supporting the nomenclature of sexually violent and predatory sexually violent youth.
A Modified Capsular Shift for Atraumatic Anterior-Inferior Shoulder Instability

Purpose To evaluate the long-term outcome of a modified inferior capsular shift procedure in patients with atraumatic anterior-inferior shoulder instability by analyzing a consecutive series of patients who had undergone a modified inferior capsular shift for this specific type of shoulder instability. Study Design Case series; Level of evidence, 4. Methods Between 1992 and 1997, 38 shoulders of 35 patients with atraumatic anterior-inferior shoulder instability that were unresponsive to nonoperative management were operated on using a modified capsular shift procedure with longitudinal incision of the capsule medially and a bony fixation of the inferior flap to the glenoid and labrum in the 1 o'clock to 3 o'clock position. The patient study group consisted of 9 men and 26 women with a mean age of 25.4 years (range, 15-55 years) at the time of surgery. The mean follow-up was 7.4 years (range, 4.0-11.4 years); 1 patient was lost to follow-up directly after surgery. The study group was evaluated according to the Rowe score. Results After 7.4 years, 2 patients experienced a single redislocation or resubluxation, 1 patient had recurrent dislocations, and 1 patient had a positive apprehension sign, which is an overall redislocation rate of 10.5%. The average Rowe score increased to 90.6 (SD = 19.7) points from 36.2 (SD = 13.5) points before surgery. Seventy-two percent of the patients participating in sports returned to their preoperative level of competition. Conclusions Results in this series demonstrate the efficacy and durability of a modified capsular shift procedure for the treatment of atraumatic anterior-inferior shoulder instability.
Migration and Maturation of Human Colonic Dendritic Cells

Dendritic cells (DC) in the colon may regulate intestinal immunity but remain poorly characterized. In this study a CD11c+HLA-DR+lin− (CD3−CD14−CD16−CD19−CD34−) population has been identified by flow cytometry in cells obtained by rapid collagenase digestion of human colonic and rectal biopsies. These day 0 (d0) CD11c+HLA-DR+lin− cells comprised ∼0.6% of the mononuclear cells obtained from the lamina propria, were endocytically active, and had the phenotype of immature DC; they were CD40+ and expressed low levels of CD83 and CD86, but little or no CD80 or CD25. Similar d0 DC populations were isolated from the colonic mucosa of healthy controls and from both inflamed and noninflamed tissue from patients with Crohn’s disease. The lamina propria also contained a population of cells capable of migrating out of biopsies during an overnight culture and differentiating into mature DC with lower levels of endocytic activity and high cell surface expression of CD40, CD80, CD86, CD83, and CD25. This mature DC population was a potent stimulator of an allogeneic mixed leukocyte (MLR). Overnight culture of cells isolated by enzymatic digestion on d0 yielded DC with a phenotype intermediate between that of the d0 cells and that of the cells migrating out overnight. Overnight culture of colonic cells in which DC and HLA-DR+lin+ cells were differentially labeled with FITC-dextran suggested that some of the maturing DC might differentiate from HLA-DR+lin+ progenitors. This study presents the first analysis of the phenotype, maturational status, and migratory activity of human gut DC.