Miracle-dz/UniFAR-FTUs huggingface dataset:
Hippocampal plasticity after a vagus nerve injury in the rat

Stimulation of the vagus nerve has been previously reported to promote neural plasticity and neurogenesis in the brain. Several studies also revealed plastic changes in the spinal cord after injuries to somatosensory nerves originating from both the brachial and lumbo-sacral plexuses. However, the neurogenic responses of the brain to the injury of the viscerosensory innervation are not as yet well understood. In the present study, we investigated whether cells in the dentate gyrus of the hippocampus respond to a chemical and physical damage to the vagus nerve in the adult rat. Intraperitoneal capsaicin administration was used to damage non-myelinated vagal afferents while subdiaphragmatic vagotomy was used to damage both the myelinated and non-myelinated vagal afferents. The 5-bromo-2-deoxyuridine (BrdU) incorporation together with cell-specific markers was used to study neural proliferation in subgranular zone, granule cell layer, molecular layer and hilus of the dentate gyrus. Microglia activation was determined by quantifying changes in the intensity of fluorescent staining with a primary antibody against ionizing calcium adapter-binding molecule 1. Results revealed that vagotomy decreased BrdU incorporation in the hilus 15 days after injury compared to the capsaicin group. Capsaicin administration decreased BrdU incorporation in the granular cell layer 60 days after the treatment. Capsaicin decreased the number of doublecortin-expressing cells in the dentate gyrus, whereas vagotomy did not alter the expression of doublecortin in the hippocampus. Both the capsaicin- and the vagotomy-induced damage to the vagus nerve decreased microglia activation in the hippocampus at 15 days after the injury. At 30 days post injury, capsaicin-treated and vagotomized rats revealed significantly more activated microglia. Our findings show that damage to the subdiaphragmatic vagus in adult rats is followed by microglia activation and long-lasting changes in the dentate gyrus, leading to alteration of neurogenesis.
Greening logistics by introducing process management– a viable tool for freight transport companies going green

ABSTRACT While the usage of process management within the freight transport industry is unknown and presumed low, it has been used within other sectors as an efficient approach for dealing with and fulfilling customer demands as well as environmental requirements. The purpose of this paper is to investigate how process management can enhance a customer focused greening in the transport and logistics sector. We present a literature review of the intersections of process management, freight transport and environmental sustainability. Furthermore, we conducted a case study of how two environmentally ambitious Swedish freight transport companies use process management to enhance environmental sustainability. We found that environmentally ambitious freight transport companies do not proactively use process management, and that workshops with topical experts and practitioners can be a way for introducing process management to enhance environmental sustainability in such companies.
Time-course of Changes in Inflammatory and Performance Responses Following a Soccer Game

Objective:To study the effects of a single soccer game on indices of performance, muscle damage, and inflammation during a 6-day recovery period. Design:Participants were assigned to either an experimental group (E, played in the game; n = 14) or a control group (C, did not participate in the game; n = 10). Setting:Data were collected on a soccer field and at the Physical Education and Sports Science laboratory of the Democritus University of Thrace before and after the soccer game. Participants:Twenty-four elite male soccer players (age, 20.1 ± 0.8 years; height, 1.78 ± 0.08 m; weight, 75.2 ± 6.8 kg). Main Outcome Measurements:Muscle strength, vertical jumping, speed, DOMS, muscle swelling, leukocyte count, creatine kinase (CK), lactate dehydrogenase (LDH), C-reactive protein (CRP), cortisol, testosterone, cytokines IL-6 and IL-1b, thioburbituric acid-reactive substances (TBARS), protein carbnyls (PC), and uric acid (UA). Results:Performance deteriorated 1 to 4 days post-game. An acute-phase inflammatory response consisted of a post-game peak of leukocyte count, cytokines, and cortisol, a 24-hour peak of CRP, TBARS, and DOMS, a 48-hour peak of CK, LDH, and PC, and a 72-hour peak of uric acid. Conclusion:A single soccer game induces short-term muscle damage and marked but transient inflammatory responses. Anaerobic performance seems to deteriorate for as long as 72-hour post-game. The acute phase inflammatory response in soccer appears to follow the same pattern as in other forms of exercise. These results clearly indicate the need of sufficient recovery for elite soccer players after a game.
Risk factors for cervical lymph node metastasis in superficial head and neck squamous cell carcinoma.

INTRODUCTION The necessity of transoral surgery for head and neck carcinoma is increasing, but its indications for the treatment of superficial head and neck carcinomas have not yet been established. This study was intended to help establish the standard indications for transoral surgery and additional therapy in patients with superficial head and neck carcinoma. METHODS Sixty-two patients with 83 superficial head and neck carcinoma underwent transoral tumor resection at the Cancer Institute Hospital between June 2006 and September 2011. We measured the tumor size and thickness, examined the gross appearance, permeation of vessels, and droplet infiltration, and analyzed the correlations between each parameter. RESULTS Sessile type of tumor on gross appearance showed a significantly higher incidence of thickness≥1000 µm than the other types. Tumor thickness≥1000 µm was associated with higher incidences of permeation of vessels, droplet infiltration, and cervical lymph node metastasis. CONCLUSIONS In superficial head and neck carcinoma, if the endoscopic gross appearance is the sessile type, tumor thickness is likely to be ≥1000 µm and risk of cervical lymph node metastasis is increased.
Enhanced Efficacy of Nigral-Striatal Cotransplants in Bilaterally Dopamine-Depleted Rats: An Anatomical and Behavioral Analysis

When embryonic ventral mesencephalic tissue containing nigral dopamine (DA) neurons is transplanted into adult DA-depleted striatum, synaptic connections form and behavioral effects are observed. This study investigated the cotransplantation of embryonic striatal tissue as a means of enhancing the innervation, survival, and functional effects of nigral transplants. Rats neonatally DA-depleted, via bilateral intraventricular injections of 6-hydroxydopamine, developed turning in response to amphetamine and stress following unilateral transplantation of either nigral or combination nigral-striatal cell suspensions. Animals with cotransplants developed higher levels of turning to both stimuli and maintained these responses for a longer period of time post-transplantation, when compared with animals receiving transplants of nigral cells alone. In addition, these combination transplants required fewer dopaminergic cells to produce a strong behavioral effect on the host. Dense patches of tyrosine hydroxylase (TH)-immunoreactive fibers were observed within the cotransplants, yet no greater outgrowth of DA fibers into host striatum was detected. Amphetamine produced widespread induction of the immediate-early gene c-fos in cells of host striatum that extended beyond the transplant-derived DA innervation. After both amphetamine and stress, Fos protein was found within both types of transplants, but these Fos-immunoreactive cells did not colocalize with TH-immunoreactive cells nor dense TH-immunoreactive patches within the grafts. Thus, cotransplanted embryonic striatal tissue augments the effects of ventral mesencephalic transplants, possibly by providing a trophic influence that enhances the function of the DA cells without increasing cell survival.
Randomized trial of screen-film versus full-field digital mammography with soft-copy reading in population-based screening program: follow-up and final results of Oslo II study.

PURPOSE To prospectively compare performance indicators at screen-film mammography (SFM) and full-field digital mammography (FFDM) in a population-based screening program. MATERIALS AND METHODS The regional ethics committee approved the study; informed consent was obtained from patients. Women aged 45-69 years were assigned to undergo SFM (n=16 985) or FFDM (n=6944). Two-view mammograms were interpreted by using independent double reading and a five-point rating scale for probability of cancer. Positive scores were discussed at consensus meetings before decision for recall. The group was followed up for 1.5 years (women aged 45-49 years) and 2.0 years (women aged 50-69 years) to include subsequent cancers with positive scores at baseline interpretation and to estimate interval cancer rate. Recall rates, cancer detection, positive predictive values (PPVs), sensitivity, specificity, tumor characteristics, and discordant interpretations of cancers were compared. RESULTS Recall rate was 4.2% at FFDM and 2.5% at SFM (P<.001). Cancer detection rate was 0.59% at FFDM and 0.38% at SFM (P=.02). There was no significant difference in PPVs. Median size of screening-detected invasive cancers was 14 mm at FFDM and 13 mm at SFM. Including cancers dismissed at consensus meetings, overall true-positive rate at baseline reading was 0.63% at FFDM and 0.43% at SFM (P=.04). Sensitivity was 77.4% at FFDM and 61.5% at SFM (P=.07); specificity was 96.5% and 97.9%, respectively (P<.005). Interval cancer rate was 17.4 at FFDM and 23.6 at SFM. The proportion of cancers with discordant double readings was comparable at FFDM and SFM. CONCLUSION FFDM resulted in a significantly higher cancer detection rate than did SFM. The PPVs were comparable for the two imaging modalities.
Augmented therapy improves outcome for pediatric high risk acute lymphocytic leukemia: Results of Children's Oncology Group trial P9906

The augmented BFM regimen improves outcome for children with NCI high acute lymphoblastic leukemia (ALL). Patient age, sex, and presenting white blood cell count (WBC) can be used to identify a subset of approximately 12% of children with B‐precursor ALL that had a 5‐year continuous complete remission (CCR) rate of only about 50% on earlier Pediatric Oncology Group (POG) trials.
The 2016 FCC Broadcast Incentive Auction

On March 29, 2016, the FCC initiated its first ever two-sided spectrum auction. The auction closed approximately one year later, having repurposed a total of 84 MHz of spectrum. The "incentive auction" included three primary components: (1) a reverse auction where broadcasters bid on the price at which they would voluntarily relinquish their current spectrum usage rights, (2) a forward ascending clock auction for flexible use wireless licenses which determined the winning bid for all licenses within a given geographic region, and (3) an assignment phase, where winning bidders from the forward auction participated in single-bid, second price sealed auctions to determine the exact frequencies individual licenses would be assigned within that geographic region. The reverse auction and the forward auction together completed a "stage". To guarantee that sufficient MHz were cleared, the auction included a "final stage rule" which, if not met, triggered a clearing of the previous stage and the start of a new stage. This rule led to a total of four stages taking place in the incentive auction before the final assignment phase took place. Even at first glance, the incentive auctions are unique among FCC spectrum auctions. Here we consider the estimated true valuation for these licenses based on market conditions. We further compare these results to more recent outcomes in previous FCC spectrum auctions for wireless services to determine if this novel auction mechanism (or possible strategic behavior in anticipation of the incentive auction) impacted auction outcomes.
Study on Pattern Distortion and DOA Estimation Performance of Crossed-Loop/Monopole Antenna in HF Radar

Crossed-loop (CL)/monopole antenna has been widely used in high-frequency ground-wave radar for ocean surface remote sensing owing to its compact size. The multiple signal classification (MUSIC) algorithm is commonly adapted to achieve a favorable angular resolution for this broad beam antenna. However, the direction of arrival (DOA) estimation is always troubled by the frequently occurring distortion of antenna pattern. Previous studies are mainly focused on the calibration by using the actual pattern or other assistant algorithms. In this paper, theoretical analysis is made to illustrate the reason for pattern distortion, which draws a conclusion that the distortion is essentially due to the different influence of environmental obstacles upon the electric field part and magnetic field part. This also implies that there is nearly no distortion between the two loops, which is validated by three different experiments. Based on this conclusion, the DOA estimation performance of the MUSIC algorithm is studied by creating a numerical relationship associating the estimation error with distortion level. Furthermore, a new method using ideal pattern is proposed to improve the estimation performance by removing the amplitude information of monopole and relying on the CL processing. Both simulation and experimental results are given to prove its validity.
Aspartame, behavior, and cognitive function in children with attention deficit disorder.

OBJECTIVE To determine the effects of large doses of aspartame on behavior, cognition, and monoamine metabolism in children with attention deficit disorder. DESIGN A randomized, double-blind, placebo-controlled crossover study of unmedicated children meeting Diagnostic and Statistical Manual of Mental Disorders (3rd ed) criteria for attention deficit disorder. SETTING Behavioral assessments were performed in the child's home by their parents and in the classroom by a teacher. Cognitive tests were administered and blood drawing was performed during a 2-day inpatient admission to our Children's Study Center. INTERVENTIONS Administration of aspartame (single morning dose, 34 mg/kg) or placebo for alternate 2-week periods. MAIN OUTCOME MEASURES Behavioral and cognitive tests included the Matching Familiar Figures Test (MFFT), Children's Checking Task (CCT), the Airplane Test, the Wisconsin Card Sorting Test (WCST), the Subjects Treatment Emergent Symptom Scale (STESS), the Multigrade Inventory for Teachers (MIT), and the Conners Behavior Rating Scale. Blood was drawn for complete blood cell count and liver function tests, as well as amino acid, methanol, formate, serotonin, and monoamine metabolite analyses, and urine was collected for measurement of catecholamine and monoamine metabolite excretion. RESULTS No clinically significant differences between aspartame and placebo were found for the STESS, MIT, or Conners ratings, or for the MFFT, CCT, WCST, or Airplane cognition tests. Also, no differences were noted for any of the biochemical measures, except for the expected increase in plasma phenylalanine and tyrosine following aspartame. CONCLUSIONS The findings indicate that aspartame at greater than 10 times usual consumption has no effect on the cognitive and behavioral status of children with attention deficit disorder. In addition, aspartame does not appear to affect urinary excretion rates of monoamines and metabolites.
Altered tissue expression pattern of cell adhesion molecules, ICAM‐1, E‐selectin and VCAM‐1, in bullous pemphigoid during methotrexate therapy

Abstract: Little is known about how eosinophils accumulate in bullous pemphigoid (BP) and why these cells rapidly disappear during immunosuppressive therapy. Eosinophils can produce cytokines such as IL‐4, IL‐5, IL‐6, IL‐10 and IL13, which can induce endothelial cells to express cellular adhesion molecules (CAMs) such as E‐selectin, vascular cell adhesion molecule‐1 (VCAM‐1) and intercellular adhesion molecule‐1 (ICAM‐1) necessary for the recruitment of eosinophils from the bloodstream to the skin. The present aim was to investigate the cellular expression of these three CAMs in serial biopsies before and during oral low‐dose methotrexate therapy. Seventy‐four biopsy specimens, 37 from active lesions and 37 from normal skin, were taken at different intervals from eight patients with bullous pemphigoid and stained immunohistochemically with specific monoclonal antibodies for these three CAMs. The expression and distribution of CAMs in the biopsies was evaluated and scored with light‐microscopic examination. The basal keratinocytes in active lesions expressed ICAM‐1. A strong VCAM‐1 expression of endothelial cells and pericytes was correlated to a perivascular inflammatory cell infiltrate that also showed intense immunoreactivity to ICAM‐1. Endothelial cell/pericytes also expressed E‐selectin strongly in the BP patients before therapy. The expression of CAMs faded during therapy and, to the best of our knowledge, this has not been previously reported. Thus we suggest that the rapid reduction of tissue eosinophils may reflect the altered pattern of cell adhesion molecules during immunosuppressive therapy, which could explain the prompt clinical improvement seen in BP patients treated with methotrexate.
A Quasi-Unipolar SPWM Full-Bridge Transformerless PV Grid-Connected Inverter with Constant Common-Mode Voltage

The unipolar sinusoidal pulsewidth modulation (SPWM) full-bridge transformerless photovoltaic inverter with ac bypass brings low conduction loss and low leakage current. In order to better eliminate the leakage current induced by the common-mode voltage, the clamping technology can be adopted to hold the common-mode voltage on a constant value in the freewheeling period. A full-bridge inverter topology with constant common-mode voltage (FB-CCV) has been derived and proposed in this paper, two unidirectional freewheeling branches are added into the ac side of the FB-CCV, and the split structure of the proposed freewheeling branches does not lead itself to the reverse-recovery issues for the freewheeling power switches and as such superjunction MOSFETs can be utilized without any efficiency penalty. The passive clamping branches consist of a capacitor divider and two diodes, is added into the dc side of the FB-CCV, therefore, the weakness of active damping branch has been overcome, and the better clamping performance has been achieved in the freewheeling period. In the dead time between the main switches and the freewheeling switches, the antiparallel diodes of diagonal main switches of the FB-CCV form the freewheeling path to clamp the common-mode voltage at a constant value, and a quasi-unipolar SPWM strategy is presented. The operation principle, differential-mode, and common-mode characteristics of the FB-CCV, Heric, H6, and HB-ZVR topologies are analyzed and compared in detail. Finally, the viability of the FB-CCV is verified by a universal prototype inverter rated at 5 kW.
Native-state energetics of a thermostabilized variant of ribonuclease HI.

Escherichia coli RNase HI is a well-characterized model system for protein folding and stability. Controlling protein stability is critical for both natural proteins and for the development of engineered proteins that function under extreme conditions. We have used native-state hydrogen exchange on a variant containing the stabilizing mutation Asp10 to alanine in order to determine its residue-specific stabilities. On average, the DeltaG(unf) value for each residue was increased by 2-3 kcal/mol, resulting in a lower relative population of partially unfolded forms. Though increased in stability by a uniform factor, D10A shows a distribution of stabilities in its secondary structural units that is similar to that of E. coli RNase H, but not the closely related protein from Thermus thermophilus. Hence, the simple mutation used to stabilize the enzyme does not recreate the balance of conformational flexibility evolved in the thermophilic protein.
Acute cardiovascular effects of bitter orange extract (p‐synephrine) consumed alone and in combination with caffeine in human subjects: A placebo‐controlled, double‐blind study

The purpose was to examine cardiovascular responses to supplementation with p‐synephrine alone and in combination with caffeine during quiet sitting. Sixteen subjects were given (in double‐blind manner) either 103 mg of p‐synephrine (S), 233 mg of caffeine +104 mg of p‐synephrine (LC + S), 240 mg of caffeine (LC), 337 mg of caffeine +46 mg of p‐synephrine (HC + S), 325 mg of caffeine (HC), or a placebo. The subjects sat quietly for 3 hr while heart rate (HR) and blood pressure were measured. Only HC + S and HC significantly increased mean systolic blood pressure (SBP) during the second hour and tended to increase mean SBP during the third hour. Mean diastolic blood pressure in S was significantly lower than the other trials during the first and second hours, and mean arterial pressure was significantly lower in S compared to the LC, LC + S, HC, and HC + S trials. No differences were observed in HR. Consumption of p‐synephrine may acutely reduce diastolic blood pressure and mean arterial pressure and not affect SBP or HR during quiet sitting. The addition of p‐synephrine to caffeine did not augment SBP or HR indicating that consumption of up to 104 mg of p‐synephrine does not induce cardiovascular stress during quiet sitting.
Impact of Labour Intensive Public Works on Youth Employment and Seasonal Migration: Evidence from Ghana

The study uses a sample of 3,876 youths to estimate the impact of Ghana’s Labour Intensive Public Works (LIPW) program on youth employment and seasonal migration using the ‘doubly-robust’ estimator that models both the treatment assignment and the outcome variable within the same framework. The data was collected in 2015 as part of the program’s impact evaluation agenda. Our results show that the project significantly increased youth employment but was less impactful on seasonal youth migration. Thus, we recommend that the labour intensive public works program should be expanded to capture more youths to increase youth employment in the country.
Fluconazole disk diffusion procedure for determining susceptibility of Candida species

A simple disk diffusion test was defined for quick determination of the susceptibility of Candida species to fluconazole. The standard macrotube dilution reference method, the fluconazole E test, and a 25-microgram fluconazole disk test were all performed with each of 250 Candida species selected to provide a broad range of fluconazole MICs. All three methods were in excellent agreement. On RPMI 1640-glucose agar, isolates with inhibition zone diameters of > or = 19 mm were all susceptible (MIC, < or = 8.0 micrograms/ml) by the E test and 94% were susceptible by the macrotube method. Strains with smaller zones were either resistant, intermediate (dose-dependent susceptibility), or susceptible by the reference methods. The disk test did not adequately separate fully resistant strains from those with dose-dependent susceptibility: additional quantitative tests are needed for the few strains that are not unequivocally susceptible by the disk method.