Miracle-dz/UniFAR-FTUs huggingface dataset:
Hybridization between two polyploid Cardamine (Brassicaceae) species in North-western Spain: discordance between morphological and genetic variation patterns.

BACKGROUND AND AIMS Hybridization is an important evolutionary phenomenon, and therefore a detailed understanding of the dynamics of interspecific gene flow and resulting morphological and genetic patterns is of widespread interest. Here hybridization between the polyploids Cardamine pratensis and C. raphanifolia at four localities is explored. Using different types of data, the aim is to provide simultaneous and direct comparisons between genotype and phenotype variation patterns in the studied hybrid populations. METHODS Evidence of hybridization has been gathered from morphology, molecular markers (amplified fragment length polymorphism and chloroplast DNA sequences), pollen viability, karyology and nuclear DNA content. KEY RESULTS All data support extensive gene flow occurring in the hybrid populations. A wide range of morphological and genetic variation is observed, which includes both parental and intermediate types. Unbalanced pollen fertility and several ploidy levels are recorded. CONCLUSIONS Incongruence reported between genotype and phenotype suggests that parental phenotypes are affected by introgression, and intermediate hybrid phenotypes can be genetically closer to one of the parents. Thus, it is evident that morphology, when used alone, can be misleading for interpreting hybridization, and critical evaluation of other data is needed.
Osteosarcoma following retinoblastoma: Age at onset and latency period

In order to assess the role of genetic predisposition in the induction of radiation-induced tumors, we performed statistical analysis on data from the literature and from our own Institute with regard to the age at onset and the latency period of osteosarcoma as the second primary tumor for retinoblastoma with or without subsequent radiotherapy. In retinoblastoma survivors who subsequently developed osteosarcoma, the age at onset of retinoblastoma was young (average of 12 months) in both unilateral and bilateral forms. This suggests that all or almost all of the patients were genetically predisposed by a mutation of one allele of the RB1 gene. For retinoblastoma patients, osteosarcomas occurred 1.2 years earlier inside than outside the radiation field. The latency period between radiotherapy and osteosarcoma onset was 1.3 years shorter inside than outside the radiation field. Interestingly, a bimodal distribution of latency periods was observed for osteosarcomas arising inside, but not outside the radiation field: 40% occurred after a short latency, while the latency of the remaining 60% was comparable to that of osteosarcoma occurring outside the radiation field. This suggests that different mechanisms may be involved in radiocarcinogenesis. A radiation-induced mutation of the second RB1 allele may be the cause of osteosarcomas occurring after a short delay, while other genes may be affected in those occurring after a longer delay.
A distinct variant of the Ehlers‐Danlos syndrome

Two unrelated males presented a distinct syndrome, consisting mainly of mental retardation, short stature, wrinkled facies, curly and fine hair, scanty eyebrows and eyelashes, telecanthus, periodontitis, hypermobility of the joints, hyperextensibility and fragility of the skin, multiple nevi, papiraceous scars, bruisability, varicose veins, pectus excavatum, winged scapulae, pes planus and bilateral cryptorchidism. Since some features were typical of Ehlers‐Danlos Syndrome (EDS), the clinical data were analyzed comparatively with the different types of EDS. The individualization of a distinct variant is concluded. Increased paternal age at the birth of both cases suggests a de novo dominant mutation.
Global Ultra-Low-Frequency Geomagnetic Pulsations Associated with the March 24, 1991 Geomagnetic Storm

On 24 March 1991, global ultra-low-frequency (ULF) pulsations (1.1 3.3 mHz) observed in the magnetosphere as well as on the ground were studied via analyzing magnetic field data obtained from a global network, comprising ground-based observatories and geosynchronous satellites. In the magnetosphere, the compressional and transverse components of the magnetic fields recorded at two satellites, GOES 6 and GOES 7, showed dominant fluctuations when they were in the vicinity of the noon sector, whereas the transverse fluctuations became dominant when they were at the dawn side. Similarly, on the ground, the H and D components had major fluctuations along with an increase in amplitude from low to high geomagnetic latitudes. In addition, the amplitude of the ULF pulsation was enhanced at the dawn and dusk sides. The geomagnetic pulsations propagated anti-sunward and were of counterclockwise and clockwise elliptical polarizations at the dawn and dusk sides respectively. The counterclockwise elliptical polarization reversed to a clockwise elliptical polarization at geomagnetic local noon and linear polarization was observed during the reversal. It appears that the analysis of the global network data not only provided us with a study of the characteristics of the waves in the magnetosphere and on the ground but also provided us with correlations between the geosynchronous and ground observations, which should be essential to the determination of possible mechanisms of this storm-related wave event.
Modeling point velocity and depth statistical distributions in steep tropical and alpine stream reaches

Statistical hydraulic models predict the frequency distributions of point hydraulic variables, relative to their reach‐averaged values, in a stream reach based on its average characteristics (e.g., discharge, depth, width, average particle size). The models initially developed in Europe have not been tested for steeper streams (>4%) with coarse grain size. We recorded water velocities and depths in 44 reaches of steep streams in tropical islands and the Alps during 69 surveys. We fitted the observed distributions of velocities and depths using a mixture of two distributions, one with low variance and the other with a high variance. Then, we predicted the mixing parameter on the basis of the reach‐averaged characteristics. We compared the observed and predicted frequencies for five classes of velocities, including a class of negative velocities, and four classes of water depths. The predictions of class frequencies have a bias of ≤5%. Our statistical model of velocity distribution predicts the frequencies of velocity classes with an explained variance between 33 and 72% for four classes of velocity and null for a class of intermediate velocity. The statistical model of depth distributions was less efficient with an explained variance between 25 and 38% for three classes of depth and null for large depths. The average Froude number, the total height of large drops relative to the reach length and the average slope are the main explanatory variables of velocity and depth distributions.
Coronary surgery in patients with low ejection fraction: mid-term results

Background: The aim of this study was to investigate the effect of preoperative low ejection fraction (≤30%) on postoperative morbidity and mortality in patients undergoing isolated on-pump coronary artery bypass grafting. We also investigated the effect of pre- and perioperative factors on survival. Methods: Between January 2002 and December 2009, 103 (6.2%) patients with an ejection fraction ≤30% and 1554 (93.8%) with an ejection fraction >30% underwent coronary artery bypass grafting. Results: In multivariate logistic regression analysis, cardiopulmonary bypass time, operation time, prolonged inotropic support, and intensive care unit stay were independent predictors of mortality in patients with low ejection fraction. Intensive care unit and hospital stays were significantly longer in these patients, and the postoperative mortality rate was significantly higher. Advanced age (≥70 years) influenced mortality during the follow-up of patients with low ejection fraction. Midterm survival was significantly reduced in patients with ejection fraction ≤30%. Smoking, prolonged inotropic support, and prolonged ventilatory support were independent predictors of midterm survival in patients with ejection fraction ≤30%. Conclusion: On-pump coronary artery bypass grafting can be performed in patients with ejection fraction ≤30%, with reasonable mortality and morbidity rates.
Post-9/11 Latino Veteran Mental Health Service Utilization

Abstract Previous research has identified disparities in mental health service utilization between Latinos and non-Latino Whites. This study aimed to examine patterns of mental health service utilization between Latino and non-Latino White post-9/11 war veterans (N = 740) enrolling for healthcare at the Veteran Affairs San Diego Healthcare System and to identify significant predictors of service utilization in Latino veterans. Predisposing, enabling, and need factors from the Behavioral Model for Health Service Utilization (BMHSU) were tested as predictors of mental health utilization. No significant differences in BMHSU factors or health service utilization emerged between Latino and non-Latino White veterans, suggesting military service may mute differences seen in civilian samples. In addition, none of the predisposing or enabling BMHSU variables significantly contributed to predict mental health utilization in Latino veterans. However, certain need variables (i.e., depression, traumatic brain injury, and pain) predicted attending one or more mental health appointments for Latino veterans. These findings reinforce the importance of assessing these particular need factors in post-9/11 Latino veterans as they have implications for treatment utilization. Furthermore, these findings suggest there may be a need to develop a model that better understands health service utilization in Latino veterans by considering the joint influence of their military identity and ethnic identity.
Who Takes the Lead in Risky Decision Making? Effects of Group Members' Risk Preferences and Prototypicality.

In two experiments, we studied the effects of (a) the extent to which group members are risk seeking in comparison with others in the group and (b) group member prototypicality (the extent to which individuals hold group-typical risk preferences) on the likelihood that group members will take the lead in risky decision making. Participants were led to believe that they engaged in a four-person group discussion and received bogus feedback about their own risk preferences, the risk preferences of the other group members, and the risk preferences of their group as a whole. In Experiment 2, we also manipulated the framing of the decision problem (gain vs. loss frame). Results supported the hypotheses that (a) more risk seeking members are more likely to take the lead and (b) prototypical members are more likely to take the lead when the problem facing the group is ambiguous (i.e., when group risk preferences and decision framing are incongruent), whereas nonprototypical members are more likely to take the lead when the problem facing the group is relatively clear-cut (i.e., when group risk preferences and decision framing are congruent). Copyright 2000 Academic Press.
Summary of Research Report: Foreign Language Teaching in the Northern part of Thailand

This research report synthesized the research data on foreign language education and use in northern Thailand. The project was conducted by seven different research groups working toward the same underlying objectives, namely to explore the current state of foreign language education and use at all academic levels and to examine satisfaction, needs, and problems related to foreign language use in the business sector in northern Thailand. The
Research on ecosystem services in Brazil: a systematic review

Studies using the ‘ecosystem services’ (ES) approach developed in Brazil based on the framework of the Millennium Ecosystem Assessment (MEA), and range from quantitative and qualitative evaluation to the development of economic instruments for payment for ecosystem services (PES) or compensation for their maintenance, mainly for hydrological services. In order to summarize current knowledge regarding ES, the structure for teaching in ES, and also to provide a basis for future research in Brazil, we carried out a systematic review of publications on ES and a study on the availability of undergraduate and graduate courses related to ES. We found 282 publications for the 2006-2017 period, which included peer-reviewed articles, books, book chapters, theses, dissertations, articles in annals and technical publications. We identified current knowledge, knowledge gaps and trends in ES researches that may guide surveys and scenario analyses for future studies, in different biomes and regions of the country. Atlantic Forest and Amazon are the most-studied biome. Most of the studies were related to the evaluation of different types of ES and to the development of methodologies for their evaluation and monitoring. The most common ES are related to biodiversity, carbon sequestration and water.
Dietary supplementation of
 Bacillus cereus
 as probiotics in Pengze crucian carp (
 Carassius auratus
 var. Pengze): Effects on growth performance, fillet quality, serum biochemical parameters and intestinal histology

This study was aimed to evaluate the effects of dietary supplementation of Bacillus cereus as probiotics on growth, fillet quality, serum biochemical parameters and intestinal histology of Pengze crucian carp (Carassius auratus var. Pengze). Fish were fed basic diet (Control group) and three diets such as CD7, CD9 and CD11 supplemented with B. cereus at dose of 10⁷, 10⁹ and 10¹¹ CFU/kg for 70 days. The results showed that supplementation of 10⁹ CFU/kg B. cereus significantly improved the growth performance compared to that in the Control group. The textures of muscle were improved by dietary supplementation of probiotics, and the hardness, gumminess, chewiness and the resilience of fish fillet increased with increasing dose of B. cereus. Total cholesterol including high‐density lipoprotein and low‐density lipoprotein level in serum decreased correspondingly with increasing level of probiotics whereas, acid phosphatase and catalase activities increased in serum. B. cereus supplementation enhanced the immunity and antioxidant capacity via an increase in acid phosphatase, alkaline phosphatase, glutathione peroxidase, reduce glutathione, and catalase, accompanied by a significant reduction in malondialdehyde. Higher intestinal fold height was observed in CD9 group than other groups. The lowest enterocyte height was exhibited in the Control group, HE values increased with increasing dose of probiotics. Moreover, dietary inclusion of B. cereus significantly affected the digestive enzymes activities in terms of higher lipase and trypase activities. In conclusion, dietary supplementation of B. cereus could promote the growth, elevate the immunity and antioxidant status of carp, and importantly improve the texture of fish fillet.
Effectiveness of probiotic therapy for the prevention of relapse in patients with inactive ulcerative colitis.

AIM To evaluate the effectiveness of probiotic therapy for suppressing relapse in patients with inactive ulcerative colitis (UC). METHODS Bio-Three tablets, each containing 2 mg of lactomin (Streptococcus faecalis T-110), 10 mg of Clostridium butyricum TO-A, and 10 mg of Bacillus mesentericus TO-A, were used as probiotic therapy. Sixty outpatients with UC in remission were randomly assigned to receive 9 Bio-Three tablets/day (Bio-Three group) or 9 placebo tablets/day (placebo group) for 12 mo in addition to their ongoing medications. Clinical symptoms were evaluated monthly or on the exacerbation of symptoms or need for additional medication. Fecal samples were collected to analyze bacterial DNA at baseline and 3-mo intervals. Terminal restriction fragment length polymorphism and cluster analyses were done to examine bacterial components of the fecal microflora. RESULTS Forty-six patients, 23 in each group, completed the study, and 14 were excluded. The relapse rates in the Bio-Three and placebo groups were respectively 0.0% vs 17.4% at 3 mo (P = 0.036), 8.7% vs 26.1% at 6 mo (P = 0.119), and 21.7% vs 34.8% (P = 0.326) at 9 mo. At 12 mo, the remission rate was 69.5% in the Bio-Three group and 56.6% in the placebo group (P = 0.248). On cluster analysis of fecal flora, 7 patients belonged to cluster I, 32 to cluster II, and 7 to cluster III. CONCLUSION Probiotics may be effective for maintaining clinical remission in patients with quiescent UC, especially those who belong to cluster I on fecal bacterial analysis.
Magnetic resonance imaging analysis of amygdala and other subcortical brain regions in adolescents with bipolar disorder.

OBJECTIVES Few studies have examined the abnormalities that underlie the neuroanatomy of bipolar disorder in youth. The aim of this study was to evaluate brain regions that are thought to modulate mood utilizing quantitative analyses of thin-slice magnetic resonance imaging (MRI) scans of adolescents with bipolar disorder. We hypothesized that adolescents with bipolar disorder would exhibit abnormalities in brain regions that are involved in the regulation of mood including the amygdala, globus pallidus, caudate, putamen, and thalamus. METHODS Bipolar adolescents (n = 23) and healthy subjects (n = 20) matched for age, race, sex, socioeconomic status, IQ, education and Tanner stage, were evaluated using the Washington University at St Louis Kiddie-Schedule for Affective Disorders and Schizophrenia (WASH-U K-SADS). Contiguous 1 mm axial T1-weighted MRI slices were obtained using a GE 1.5 T MR scanner. Regions of interest (ROI) included total cerebral volume, amygdala, globus pallidus, caudate, putamen, and thalamus. RESULTS Total cerebral volume was smaller in bipolar adolescents than in healthy adolescents. A MANCOVA revealed a significant group difference in overall ROI volumes after adjusting for total cerebral volume. Specifically, adolescents with bipolar disorder exhibited smaller amygdala and enlarged putamen compared with healthy subjects. CONCLUSIONS Our findings indicate that adolescents with bipolar disorder exhibit abnormalities in some of the brain regions that are thought to be involved in the regulation of mood. Additional structural and functional neuroimaging investigations of children, adolescents, and adults with bipolar disorder are necessary to clarify the role of these brain regions in the neurophysiology of adolescent bipolar disorder.
Formulation of Flood Disaster Classification Standards Based on Fuzzy Clustering Iterative Model and Chaotic Differential Evolution Algorithm

In allusion to the problem of formulation of flood disaster classification standards, this paper proposes a method to deal with a fuzzy clustering iterative model and chaotic differential evolution algorithm. After analyzed a flooding example with fuzzy clustering iterative model, the classification standards was developed according to the optimal fuzzy clustering center matrix and the standard was verified by projection pursuit cluster model. Data analysis showed that the classification is reasonable and effective.
Bactericidal versus bacteriostatic antibiotic therapy of experimental pneumococcal meningitis in rabbits.

A rabbit model of pneumococcal meningitis was used to examine the importance of bactericidal vs. bacteriostatic antimicrobial agents in the therapy of meningitis 112 animals were infected with one of two strains of type III Streptococcus pneumoniae. Both strains were exquisitely sensitive to ampicillin, minimum inhibitory concentration (MIC)/minimum bactericidal concentration (MBC)<0.125 mug/ml. The activity of chloramphenicol against the two strains varied: strain(1)-MIC 2 mug/ml, MBC 16 mug/ml; strain(2)-MIC 1 mug/ml, MBC 2 mug/ml. Animals were treated with either ampicillin or chloramphenicol in dosages that achieved a peak bactericidal effect in cerebrospinal fluid (CSF) for ampicillin against both strains. Two different dosages were used for chloramphenicol. The first dosage achieved a peak CSF concentration of 4.4+/-1.1 mug/ml that produced a bacteriostatic effect against strain(1) and bactericidal effect against strain(2). The second dosage achieved a bactericidal effect against both strains (mean peak CSF concentration 30.0 mug/ml). All animals were treated intramuscularly three times a day for 5 d. CSF was sampled daily and 3 d after discontinuation of therapy for quantitative bacterial cultures. Results demonstrate that only antimicrobial therapy that achieved a bactericidal effect in CSF was associated with cure. Over 90% of animals treated with one of the bactericidal regimens (i.e., animals in which the bacterial counts in CSF dropped >5 log(10) colony-forming units [cfu]/ ml after 48 h) had sterile CSF after 5 d of treatment. On the other hand, the regimen that achieved bacteriostatic concentrations (CSF drug concentrations between the MIC and MBC) produced a drop of 2.4 log(10) cfu/ml by 48 h; however, none of the animals that survived had sterile CSF after 5 d. These studies clearly demonstrate in a strictly controlled manner that maximally effective antimicrobial therapy of experimental pneumococcal meningitis depends on achieving a bactericidal effect in CSF.
Performance Analysis of ZF Pre-Coder and Extended Joint Channel Estimation Method in Downlink CDMA with HPA

In the down link scenario of CDMA systems, Linear De-Correlating Detectors (LDDs) provide satisfactory Symbol Error Rates (SERs), but they require all active users’ spreading sequences, which is impractical from privacy perspective. To overcome this impracticality, a simple matched filter receiver is considered in this paper. However, this receiver degrades the SER due to Multiple Access Interference (MAI). So, a Zero Force (ZF) pre-coder is employed in the transmitter to improve the SER. Moreover, we consider the Non-Linear Distortion (NLD) caused by the High Power Amplifier (HPA) of the Base Station (BS) due to large Peak to Average Power Ratio (PAPR) of the composite of CDMA signals. We analyze the down link of the proposed system to derive an equation for its SER. Theoretical analysis and numerical results show that the ZF pre-coder increases the total degradation of the link significantly compared with that of the LDD. So, as a solution, rather than ZF pre-coder, we propose a new method called extended joint channel estimation in which joint estimation of channel gains and LDD operator are used by the mobile station (MS). In this case, the BS transmits row k of LDD operator to MS k. Simulation results show that the SER of this new proposed method is close to that of the LDD in Additive White Gaussian Noise (AWGN) channel with the added advantage of no need for spreading sequences of all co-users.
Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria

ABSTRACT A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with Bifidobacterium genus- and species-specific primers. Real-time PCR detection of serially diluted DNA extracted from cultured bifidobacteria was linear for cell counts ranging from 106 to 10 cells per PCR assay. It was also found that the method was applicable to the detection of Bifidobacterium in feces when it was present at concentrations of >106 cells per g of feces. Concerning the distribution of Bifidobacterium species in intestinal flora, the Bifidobacterium adolescentis group, the Bifidobacterium catenulatum group, and Bifidobacterium longum were found to be the three predominant species by examination of DNA extracted from the feces of 46 healthy adults. We also examined changes in the population and composition of Bifidobacterium species in human intestinal flora of six healthy adults over an 8-month period. The results showed that the composition of bifidobacterial flora was basically stable throughout the test period.