Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of CINtec PLUS® testing as an adjunctive test in ASC‐US diagnosed SurePath® preparations

The CINtec PLUS® system is an immunohistochemical cocktail composed of antibodies against p16INK4a (surrogate of HPV infection) and Ki‐67 (proliferation marker) meant to improve the sensitivity and specificity for detecting high‐grade dysplasia (HGD). In the presence of dysplasia, a red chromogen marks Ki‐67 expression in the nucleus and a brown chromogen marks cytoplasmic p16INK4a expression. Only cells showing dual staining are interpreted as positive. This retrospective study examined the performance of CINtec PLUS testing when performed on ASC‐US diagnosed samples. Comparison was made to high‐risk HPV DNA test results and colposcopic biopsy results. Technical considerations in the interpretation of this immunohistochemical stain are additionally discussed. CINtec PLUS showed modest sensitivity (64%) and specificity (53%) in identifying the presence of HGD at surgical biopsy. Positive and negative predictive values for HGD were 28% and 83%, respectively. HR‐HPV DNA test yielded sensitivity of 100% and specificity of 21%. During interpretation, squamous metaplasia and endocervical cells were seen to show individual staining for p16INK4a or Ki‐67. Individual staining, when present within three dimensional cellular groups common to SurePath® preparations, can be time‐intensive to interpret necessitating thoughtful examination at high power. The Pap test with HR‐HPV DNA testing is a highly sensitive test. A specific test is needed to prevent false positives and over treatment. The CINtec® system provides a modest increase in specificity beyond HR‐HPV DNA testing. Future study of its appropriateness and cost‐ffectiveness in a treatment algorithm are warranted. Diagn. Cytopathol. 2013. © 2011 Wiley Periodicals, Inc.
Light‐Induced Retinal Degeneration Is Prevented by Zinc, a Component in the Age‐related Eye Disease Study Formulation †

Mineral supplements are often included in multivitamin preparations because of their beneficial effects on metabolism. In this study, we used an animal model of light‐induced retinal degeneration to test for photoreceptor cell protection by the essential trace element zinc. Rats were treated with various doses of zinc oxide and then exposed to intense visible light for as long as 8 h. Zinc treatment effectively prevented retinal light damage as determined by rhodopsin and retinal DNA recovery, histology and electrophoretic analysis of DNA damage and oxidized retinal proteins. Zinc oxide was particularly effective when given before light exposure and at doses two‐ to four‐fold higher than recommended by the age‐related eye disease study group. Treated rats exhibited higher serum and retinal pigment epithelial zinc levels and an altered retinal gene expression profile. Using an Ingenuity database, 512 genes with known functional annotations were found to be responsive to zinc supplementation, with 45% of these falling into a network related to cellular growth, proliferation, cell cycle and death. Although these data suggest an integrated and extensive regulatory response, zinc induced changes in gene expression also appear to enhance antioxidative capacity in retina and reduce oxidative damage arising from intense light exposure.
Survey of urologic laparoscopic practice patterns in Saudi Arabia.

BACKGROUND AND PURPOSE Laparoscopic surgery is taking a greater role in the management of many urologic diseases. We performed a survey whose aim was to define laparoscopic practice patterns among urologists in Saudi Arabia. MATERIALS AND METHODS In March 2009, detailed questionnaires about urologic laparoscopic practice patterns were distributed to 352 urologists who were working in Saudi Arabia. The questions related to age, residency training, and amount of laparoscopy performed. RESULTS The overall response rate was 42%. A total of 21% of certified urologists in Saudi Arabia performed no laparoscopy, 24.8% devoted less than 5% of their practice time, and 54.3% devoted 5% or more of their practice time to laparoscopic surgery. Of the respondents, 27.5% stated that they had adequate training during residency to perform laparoscopy. Both age and time in practice were inversely related to the amount of time devoted to laparoscopy (P = 0.001). Of those who were affiliated with a university, 70% devoted more than 5% of their operative time to laparoscopy. Urologists in the Ministry of Health were the least to perform a significant volume of laparoscopy. The two most important reasons mentioned for performing laparoscopy were a faster recovery period and reduced morbidity. The main laparoscopic procedures, according to the proportion of urologists who perform laparoscopic surgery, were varicocelectomy, 38%; simple nephrectomy, 27%; renal cyst decortications, 23%; adrenalectomy, 20%; radical nephrectomy, 20%; pyeloplasty, 18%; and orchidopexy, 17%. CONCLUSIONS Urologic laparoscopic practice in Saudi Arabia is still in its early stages. Accordingly, more laparoscopic procedures should be implemented in the local training programs.
Accuracy of CT-guided needle biopsy of musculoskeletal neoplasms.

OBJECTIVE The purpose of our study was to assess the accuracy of CT-guided biopsy of musculoskeletal neoplasms with respect to technique, anatomic site, and histology. MATERIALS AND METHODS During a 3-year period (January 1992 to December 1994), 176 core needle biopsies and 45 fine-needle aspirations were performed under CT guidance on patients with musculoskeletal neoplasms. To assess the accuracy of these procedures, we compared the diagnosis at biopsy with the final diagnosis as determined at the time of definitive treatment of the lesion. All biopsy findings were categorized as a primary malignancy (excluding round cell lesions), round cell lesion, local recurrence, or metastatic carcinoma. In addition, each lesion was analyzed according to which biopsy technique was used, whether frozen tissue section or rapid cytologic evaluation was used, and at which anatomic site the mass was found. RESULTS The accuracy for needle biopsy was 93% and that for fine-needle aspiration was 80%. The complication rate for both techniques was less than 1%. Accuracy rates for the four categories of primary malignancy, round cell lesion, local recurrence, and metastatic carcinoma were 87%, 75%, 94%, and 100%, respectively. The mismatch rates were similar in soft-tissue lesions (5/52) and bone lesions (16/169). Diminished accuracy was associated with round cell lesions (20%) and lesions located in the spine or the perivertebral region (20%). Nondiagnostic and insufficient specimens were found in 18 (8%) of the 221 patients. CONCLUSION. CT-guided biopsy of musculoskeletal malignancies is a safe and effective procedure if performed by a team of clinicians, pathologists, and radiologists who possess subspecialty expertise.
Cardiac Device Complications in the Cognitively Impaired

Patients with preexisting mild cognitive impairment or dementia may be at increased risk for developing cardiac device complications due to an impaired ability to follow postimplant care instructions. We sought to determine whether rates of infection, lead dislodgement, or appropriate or inappropriate implantable cardioverter defibrillator (ICD) shocks are increased in this population.
The Cupboard is Full: Public Finances for Public Services in the Global South

We are constantly told that there is no money for badly needed public services around the world. But who said the cupboard was bare? There is ample evidence that large pools of public monies exist for investment in public infrastructure, with public pension funds and sovereign wealth funds being two examples. Currently, these funds are being directed toward large-scale, capital-intensive, high-return projects aimed primarily at well-off urban residents and the private sector. Lessons from the financial crisis show that such funds could actually realize greater long-term returns from investment in public service provision while avoiding the politically controversial and contradictory practice of using public sector funds to support privatization. We make the case for using public pension funds and sovereign wealth funds for socially responsible investments in the global South, in support of essential public services.
Negative Regulation of Chk2 Expression by p53 Is Dependent on the CCAAT-binding Transcription Factor NF-Y*

The kinase Chk2 and tumor suppressor p53 participate in an ill defined regulatory interaction in mammalian cells. The abundance of Chk2 mRNA and protein has now been shown to be decreased by the induction of p53 in Saos2 cells. Ionizing radiation also triggered the phosphorylation and subsequent down-regulation of Chk2 in human colorectal HCT116 (p53+/+) cancer cells; irradiation of its isogenic mutant HCT116 (p53–/–) cells, which lack functional p53, induced Chk2 phosphorylation but not its down-regulation. In addition, HCT116 (p53+/+) cells constitutively expressing a dominant negative p53 (V143A) failed to suppress Chk2 expression after irradiation. Reporter gene assays in HCT116 (p53+/+) cells revealed that wild-type p53 repressed, whereas a dominant negative p53 mutant increased, the activity of the human Chk2 gene promoter. Mutational analysis showed that a CCAAT box located between nucleotides –152 and –138 of the promoter was responsible for its negative regulation by p53. Electrophoretic mobility shift assays demonstrated that the transcription factor NF-Y binds to this CCAAT sequence. A dominant negative mutant of NF-YA abolished the effect of p53 on Chk2 promoter activity. These results suggest that p53 negatively regulates Chk2 gene transcription through modulation of NF-Y function and that this regulation may be important for reentry of cells into the cell cycle after DNA damage is repaired.
Outcome of dental implants in partially edentulous patients with and without a history of periodontitis: a 5-year interim analysis of a cohort study.

PURPOSE To evaluate the outcome of dental implants placed in partially edentulous patients with a history of severe periodontitis (SP), moderate periodontitis (MP) and no periodontitis (NP). MATERIALS AND METHODS Sixty-two partially edentulous patients were consecutively enrolled in this study. Patients were divided into three groups according to their initial periodontal conditions, assessed with a modified Periodontal Screening and Recording (PSR) index: 26 patients were in the SP group, 7 in the MP group, and 29 in the NP group. Patients requiring periodontal treatment were treated prior to implantation. Various dental implants and procedures were used. In the SP group 129 implants were placed, 26 were placed in the MP group, and 72 in the NP group. Outcome measures were prosthetic success, implant survival, prevalence of peri-implantitis and mean peri-implant bone level changes on periapical radiographs. RESULTS Six patients dropped out, two from each group, at 5 years. Two implants and their prosthesis failed with peri-implantitis in one patient and two implants were successfully treated for periimplantitis in another patient in the SP group. After 5 years, patients affected by SP and MP lost on average twice the amount of peri-implant bone compared with healthy patients (2.6 mm versus 1.2 mm). This difference was highly statistically significant. CONCLUSIONS Patients with a history of SP and MP lose more peri-implant bone than periodontally healthy patients and might be at higher risk for peri-implantitis and implant failures.
HetNets with cognitive small cells: user offloading and distributed channel access techniques

Heterogeneous networks, consisting of macrocells overlaid with small cells (e.g., femtocells, picocells, microcells) provide a fast, flexible, cost-efficient, and fine-tuned design and expansion for existing cellular wireless networks to satisfy the ever increasing demand for network capacity. In HetNets, small cells serve as offloading spots in the radio access network to offload users and their associated traffic from congested macrocells. However, due to their large-scale deployment in random locations, limited transmit power, and the lack of complete coordination, the coexistence and efficient operation of small cells is very challenging. In this article, we discuss the coexistence challenges posed to small cells and show that, with cognition capabilities (e.g., achieved through spectrum sensing), small cells can overcome the posed challenges and efficiently coexist in a multitier cellular wireless network. Then we discuss a statistical tool, stochastic geometry, to model and analyze heterogeneous cellular networks. We give two examples where the stochastic geometry tools can be exploited to obtain insightful design guidelines. First, we exploit stochastic geometry to evaluate the load of each network tier and study different offloading techniques used to control this load. Second, we exploit stochastic geometry to maximize frequency reuse efficiency through spectrum sensing design for channel access and compare two channel access techniques based on spectrum sensing.
Outcomes of pregnancy in women with tetralogy of Fallot*

Abstract Background Surgical results after repair of tetralogy of Fallot have remained excellent for the last decades, with current long-term rates of survival over 95%. Since functional capacity, quality of life, and social interactions are basically normal in this large group of patients, pregnancy obviously becomes a relevant issue for the female subgroup. In consequence, adequate obstetrical and cardiological management of pregnancy is particularly important. Objective To describe the outcomes of pregnancy, and fertility, in a series of women who underwent surgery for tetralogy of Fallot in a single centre. Methods and results We obtained data from hospital records, national registries, and questionnaires on 78 women who underwent surgical correction of tetralogy of Fallot between 1972 and 1992. Of 58 women who reached an age of at least 18 years, with 45 of this cohort currently surviving, 13 having died as adults, there were 54 pregnancies in 25 women. The recorded rate of spontaneous abortion was 15%, and infertility rate was 3.4%. There have been 41 life births, with a median weight at birth of 3.2 kg. Only 1 newborn was small for gestational age, and no one was born before the 36th week. The recurrence rate of congenital heart disease was high, at 9.8%. Cardiac complications during or after pregnancy were not observed, and only one woman had pre-eclampsia. Conclusions Pregnancy is well tolerated in women with tetralogy of Fallot, and an excellent neonatal outcome is expected. The recurrence risk of congenital cardiac disease, most often tetralogy of Fallot, is high.
Fluorescence in situ hybridization for detecting transitional cell carcinoma: implications for clinical practice

To determine the diagnostic sensitivity of genetic studies using fluorescence in situ hybridization (FISH) for detecting both new and recurrent cases of transitional cell carcinoma (TCC) in a routine clinical practice setting, as bladder cancer has a significant risk of recurrence and progression to invasive disease and thus sensitive surveillance testing is very important.
The Impact of Incentives on Intrinsic and Extrinsic Motives for Fitness-Center Attendance in College First-Year Students

Purpose. A criticism of incentives for health behaviors is that incentives undermine intrinsic motivation. The objective of this study was to determine the impact of monetary incentive provision on participation motives for exercise in first-year college students at a northeastern public university. Design. Randomized-controlled trial. Setting. Public university in the Northeastern United States. Subjects. One hundred seventeen first-year college students. Intervention. Participants were randomized to one of three conditions: a control condition receiving no incentives for meeting fitness-center attendance goals; a discontinued-incentive condition receiving weekly incentives during fall semester 2011, and no incentives during spring semester 2012; or a continued-incentive condition receiving weekly incentives during fall semester, and incentives on a variable-interval schedule during spring semester. Measures. The Exercise Motivation Inventory 2 measured exercise participation motives at baseline, end of fall semester, and end of spring semester. Fitness-center attendance was monitored by using ID-card check-in/check-out records. Analysis. Repeated-measures analyses using linear mixed models with first-order autoregressive covariance structures were run to compare motive changes in the three conditions. Results. Participation motives of Enjoyment and Revitalization associated with intrinsic motivation did not decrease significantly over time in any of the conditions, F(4, 218) = 2.25, p = . 065 and F(4, 220) = 1.67, p = . 16, respectively. Conclusion. Intrinsically associated participation motives for exercise did not decrease with incentive provision. Therefore, incentives may encourage fitness-center attendance without negatively impacting participation motives for exercise.
Circadian changes of serum prolactin levels and tuberoinfundibular dopaminergic neuron activities in ovariectomized rats treated with or without estrogen: the role of the suprachiasmatic nuclei.

Variations of serum prolactin (PRL) levels and activities of tuberoinfundibular dopaminergic (TIDA) neurons during the afternoon of ovariectomized (OVX) rats treated with or without estrogen were determined in this study. Long-term OVX rats treated with or without polyestradiol phosphate (0.1 mg/rat, s.c.) were decapitated every hour from 10.00 to 19.00 h (except 11.00 and 13.00 h). Serum PRL and median eminence (ME) dihydroxyphenylacetic acid (DOPAC) or dihydroxyphenylalanine (DOPA) levels were determined by radioimmunoassay and high performance liquid chromatography plus electrochemical detection, respectively. A prominent PRL surge started and peaked around 14.00-15.00 h, and remained significantly higher than levels of 10.00 and 12.00 h throughout the afternoon. Significant decreases of ME DOPAC and DOPA concentrations were also observed between 14.00 and 19.00 h. In OVX rats with no estrogen replacement, no PRL surge was observed and the changes of ME DOPAC concentrations during the afternoon were not significant except for that at 17.00 h. The ME DOPA accumulation, however, exhibited significantly lower levels from 14.00 to 19.00 h than that at 12.00 h, indicating that an endogenous rhythm for DA synthesis existed in OVX rats. In estrogen-treated OVX rats bearing bilateral lesions of the suprachiasmatic nuclei, both changes in serum PRL level and TIDA neuron activity were abolished. We conclude that an endogenous rhythm of the activities of TIDA neurons may exist in both OVX and OVX plus estrogen-treated rats. The rhythm is regulated by the suprachiasmatic nuclei and may be amplified by estrogen for the induction of PRL surge.
Another look at heavy episodic drinking and alcohol use disorders among college and noncollege youth.

OBJECTIVE To estimate rates of heavy episodic drinking, alcohol abuse and alcohol dependence among U.S. adults 18-29 years of age and determine the relationship of these rates to student status and residence. METHOD The analysis is based on data from a subsample of U.S. adults 18-29 years of age (N = 8666; 4849 female) who were interviewed as part of the 2001-02 National Epidemiologic Survey on Alcohol and Related Conditions (N = 43,093). Data were collected in personal interviews from a representative sample of adults 18 and older, living in households and selected group quarters in the United States, including Alaska, Hawaii and the District of Columbia. RESULTS Of all adults 18-29 years of age, 73.1% reported any drinking in the past year, 39.6% reported any heavy episodic drinking, 21.1% reported heavy drinking more than once a month and 11.0% reported heavy drinking more than once a week. Among past-year drinkers, these correspond to rates of 54.3% for any heavy episodic drinking, 28.9% for heavy drinking more than once a month and 15.0% for heavy drinking more than once a week. Although rates of heavy episodic drinking were slightly higher for college students than for noncollege students (p < .01), differences according to place of residence were greater than differences according to student status. Overall, 7.0% of adults ages 18-29 met the DSM-IV criteria for alcohol abuse in the past year, and 9.2% met the criteria for alcohol dependence. The prevalence of abuse was highest among students living off campus (p < .01), and rates of dependence were highest among students living on campus (p < .01). CONCLUSIONS Heavy episodic drinking and alcohol use disorders are youth as well as college phenomena. Prevention campaigns targeted at all youth are needed to supplement interventions conducted at the campus level.
General medical and psychiatric perspectives on somatoform disorders: separated by an uncommon language

Purpose of review While Diagnostic and Statistical Manual of Mental Disorders-IV provided updated definitions and diagnostic criteria for the diagnoses subsumed under the category of somatoform disorders, various nosological questions are still unresolved. We assessed current research activity on important key concepts related to nosology, validity, and reliability of the Diagnostic and Statistical Manual of Mental Disorders-IV somatoform diagnoses. Recent findings The initial Medline search for articles from 2000 through 2005 yielded a total of 912 articles. After checking for redundancy and excluding publications using other than Diagnostic and Statistical Manual of Mental Disorders-IV diagnostic criteria, a total of 101 articles was further reviewed. Heterogeneity of papers precluded statistical approaches; thus, a descriptive and narrative review was pursued. Summary It appears that applying the specific categories and criteria as proposed by Diagnostic and Statistical Manual of Mental Disorders-IV only captures a small proportion of the phenomena of interest at the interface of medicine and psychiatry. A paradigm shift towards ‘general medical/psychiatry interface disorders’ in exchange for the current dichotomy of ‘mind’ vs. ‘body’ disorders, may reduce stigma associated with current diagnostic labeling, improve user acceptability, and stimulate research in this important but still fragmented clinical area that still lacks agreed-upon diagnostic definitions.