Miracle-dz/UniFAR-FTUs huggingface dataset:
Ligature-induced peri-implantitis in mice.

BACKGROUND AND OBJECTIVE Peri-implantitis has a prevalence of 11-47%, involves destruction of peri-implant bone and may lead to implant loss. A detailed understanding of the pathogenesis of peri-implantitis is lacking. The objective of this study was to develop a murine model of experimental peri-implantitis. MATERIAL AND METHODS Machined, smooth-surface, screw-shaped titanium implants were placed in the healed alveolar bone of the left maxillary molars of C57BL/6J male mice, 8 wk after tooth extraction. Peri-implantitis was induced by securing silk ligatures around the head of the implant fixtures. Implant survival and peri-implant bone levels were analyzed by micro-computed tomography (micro-CT) scans and histology, 12 wk after ligature placement. RESULTS Implant survival was 60% (six of 10) for implants with ligatures and 100% (eight of eight) for controls. Micro-CT revealed significantly greater bone loss around the implants that received ligatures and that survived, compared with controls. The radiographic findings were confirmed via histology and toluidine blue staining. CONCLUSION This study describes a murine model of experimental peri-implantitis around screw-shaped titanium implants placed in the edentulous alveolar bone. This model should be a useful tool to dissect pathogenic mechanisms of peri-implantitis and evaluate potential treatment interventions.
Heritability of periodontal bone loss in mice.

BACKGROUND Periodontitis is an inflammatory disease of the periodontal tissues that compromises tooth support and can lead to tooth loss. Although bacterial biofilm is central in disease pathogenesis, the host response plays an important role in the progression and severity of periodontitis. Indeed, clinical genetic studies indicate that periodontitis is 50% heritable. In this study, we hypothesized that lipopolysaccharide (LPS) injections lead to a strain-dependent periodontal bone loss pattern. MATERIAL AND METHODS We utilized five inbred mouse strains that derive the recombinant strains of the hybrid mouse diversity panel. Mice received Porphyromonas gingivalis-LPS injections for 6 wk. RESULTS AND CONCLUSION Micro-computed tomography analysis demonstrated a statistically significant strain-dependent bone loss. The most susceptible strain, C57BL/6J, had a fivefold higher LPS-induced bone loss compared to the most resistant strain, A/J. More importantly, periodontal bone loss revealed 49% heritability, which closely mimics periodontitis heritability for patients. To evaluate further the functional differences that underlie periodontal bone loss, osteoclast numbers of C57BL/6J and A/J mice were measured in vivo and in vitro. In vitro analysis of osteoclastogenic potential showed a higher number of osteoclasts in C57BL/6J compared to A/J mice. In vivo LPS injections statistically significantly increased osteoclast numbers in both groups. Importantly, the number of osteoclasts was higher in C57BL/6J vs. A/J mice. These data support a significant role of the genetic framework in LPS-induced periodontal bone loss and the feasibility of utilizing the hybrid mouse diversity panel to determine the genetic factors that affect periodontal bone loss. Expanding these studies will contribute in predicting patients genetically predisposed to periodontitis and in identifying the biological basis of disease susceptibility.
Subgingival bacterial community profiles in HIV-infected Brazilian adults with chronic periodontitis.

BACKGROUND AND OBJECTIVE To compare the subgingival microbial diversity between non-HIV-infected and HIV-infected individuals with chronic periodontitis using denaturing gradient gel electrophoresis (DGGE). MATERIAL AND METHODS Thirty-two patients were selected: 11 were HIV-infected and 21 were non-HIV-infected, and all had chronic periodontitis. Periodontal measurements included probing depth, clinical attachment level, visible supragingival biofilm and bleeding on probing. Subgingival biofilm samples were collected from periodontal sites (50% with probing depth ≤ 4 mm and 50% with probing depth ≥ 5 mm) and whole-genomic-amplified DNA was obtained. The DNA samples were subjected to amplification of a 16S rRNA gene fragment using universal bacterial primers, followed by DGGE analysis of the amplified gene sequences. RESULTS The non-HIV-infected group presented higher mean full-mouth visible supragingival biofilm (p = 0.004), bleeding on probing (p = 0.006), probing depth (p < 0.001) and clinical attachment level (p = 0.001) in comparison with the HIV-infected group. DGGE analysis revealed 81 distinct bands from all 33 individuals. Banding profiles revealed a higher diversity of the bacterial communities in the subgingival biofilm of HIV-infected patients with chronic periodontitis. Moreover, cluster and principal component analyses demonstrated that the bacterial community profiles differed between these two conditions. High interindividual and intra-individual variability in banding profiles were observed for both groups. CONCLUSION HIV-infected patients with chronic periodontitis present greater subgingival microbial diversity. In addition, the bacterial communities associated with HIV-infected and non-HIV-infected individuals are different in structure.
Forest refugia in Western and Central Africa as ‘museums’ of Mesozoic biodiversity

The refugial speciation model, or ‘species pump’, is widely accepted in the context of tropical biogeography and has been advocated as an explanation for present species distributions in tropical Western and Central Africa. In order to test this hypothesis, a phylogeny of the cryptic arachnid order Ricinulei, based on four nuclear and mitochondrial DNA markers, was inferred. This ancient clade of litter-dwelling arthropods, endemic to the primary forests of Western and Central Africa and the Neotropics, might provide insights into the mode and tempo of evolution in Africa. Twenty-six African ricinuleid specimens were sampled from eight countries spanning the distribution of Ricinulei on the continent, and analysed together with Neotropical samples plus other arachnid outgroups. The phylogenetic and molecular dating results suggest that Ricinulei diversified in association with the fragmentation of Gondwana. The early diversification of Ricinoides in Western and Central Africa around 88 (±33) Ma fits old palaeogeographical events better than recent climatic fluctuations. Unlike most recent molecular studies, these results agree with fossil evidence, suggesting that refugia may have acted as ‘museums’ conserving ancient diversity rather than as engines generating diversity during successive episodes of climatic fluctuation in Africa.
Self-Reported Physical Activity Among Older Acadians

Abstract North American society is aging. Individuals who are older are often confronted with a variety of issues such as transportation, health care, adequate income, and maintaining physical activity levels. Older adults are not a homogeneous group, for instance, Canadian society has been mainly composed of two cultures, English and French. The purpose of this study was to examine the contribution of demographic, health, and lifestyle variables on the self-reported physical activity of 1,281 older Acadians, residing in Eastern Canada. The demographic variables included age, sex, and education, while the health variables comprised self-perceived health and functional limitations. Four lifestyle variables were included: control, smoking, drinking, and social activity. A hierarchical regression analysis determined the contribution of these variables on self-reported physical activity. All variables were significant except for age, control and drinking, which accounted for 17% of the variance. Although health accounts for a greater percentage of the variance, lifestyle variables did contribute significantly to the model. The findings served to reinforce the need to further examine the contribution of lifestyle variables on self-reported physical activity in older Acadians.
Increasing neonicotinoid use and the declining butterfly fauna of lowland California

The butterfly fauna of lowland Northern California has exhibited a marked decline in recent years that previous studies have attributed in part to altered climatic conditions and changes in land use. Here, we ask if a shift in insecticide use towards neonicotinoids is associated with butterfly declines at four sites in the region that have been monitored for four decades. A negative association between butterfly populations and increasing neonicotinoid application is detectable while controlling for land use and other factors, and appears to be more severe for smaller-bodied species. These results suggest that neonicotinoids could influence non-target insect populations occurring in proximity to application locations, and highlights the need for mechanistic work to complement long-term observational data.
A trade-off between reproductive investment and maternal cerebellum size in a precocial bird

Natural selection favours increased investment in reproduction, yet considerable variation in parental investment is observed in natural populations. Life-history theory predicts that this variation is maintained by a trade-off between the benefits of increased reproductive investment and its associated costs for the parents. The nature of these costs of reproduction, however, remains poorly understood. The brain is an energetically highly expensive organ and increased reproductive investment may, therefore, negatively affect brain maintenance. Using artificial selection lines for high and low prenatal maternal investment in a precocial bird, the Japanese quail (Coturnix japonica), we provide experimental evidence for this hypothesis by showing that increased prenatal provisioning negatively affects the size of a particular brain region of the mother, the cerebellum. Our finding suggests that cognitive demands may constrain the evolution of parental investment, and vice versa, contributing to the maintenance of variation in reproductive behaviour in animal populations.
Safety, Feasibility and Family Experiences of Preventive Interventions for Children and Families with Parental Depression

We report on the safety and feasibility and family members’ experiences of two public health interventions for families with depressed parents. Depressed patients (N = 119) with children were randomised into either a one- or two-session discussion conducted by a clinician with parents, or a family intervention involving the whole family. Family members’ experiences were assessed by questionnaires. Clinicians provided information on intervention fidelity. The interventions proved safe and feasible, and were delivered with fidelity. Parents and children reported positive working relationships, increases in family understanding and decrease of worry. Children reported that they would recommend the intervention to others. Parents reported enhanced self-understanding, parenting and future orientation. While both interventions were received positively, parental perceptions of the family intervention were more positive. Child-centred public health interventions can be trained and implemented in adult mental health settings. Such approaches are valued by parents and children.
Establishment and maintenance of aphid endosymbionts after horizontal transfer is dependent on host genotype

Animal-associated microbial communities have important effects on host phenotypes. Individuals within and among species differ in the strains and species of microbes that they harbour, but how natural selection shapes the distribution and abundance of symbionts in natural populations is not well understood. Symbionts can be beneficial in certain environments but also impose costs on their hosts. Consequently, individuals that can or cannot associate with symbionts will be favoured under different ecological circumstances. As a result, we predict that individuals within a species vary in terms of how well they accept and maintain symbionts. In pea aphids, the frequency of endosymbionts varies among host-plant-associated populations (‘biotypes’). We show that aphid genotypes from different biotypes vary in how well they accept and maintain symbionts after horizontal transfer. We find that aphids from biotypes that frequently harbour symbionts are better able to associate with novel symbionts than those from biotypes that less frequently harbour symbionts. Intraspecific variation in the ability of hosts to interact with symbionts is an understudied factor explaining patterns of host–symbiont association.
Tuberculosis knowledge, perception and practice among patent medicine vendors in south-east, Nigeria.

Introduction: Patent medicines vendors (PMV) are ubiquitous in developing nations like Nigeria. They have been engaged to improve public health indices for health conditions like malaria. Tuberculosis case detection has remained sub-optimal in Nigeria. Little is known of the PMV interaction with tuberculosis control in Enugu state, south east Nigeria. The study explores tuberculosis knowledge, perception and practices among urban and rural based patent medicine vendors. Method: A comparative cross-sectional descriptive study with multi-stage sampling technique. Following stratification, Enugu north (urban) and Nkanu West (rural) LGAs were selected. All registered patent medicine vendors (urban 90, rural 70) were identified for the study. Eighty three (83) urban and 66 rural PMV participated. Data was collected with a semi-structured interviewer administered questionnaire and analyzed using SPSS version eleven and Microsoft excel. Results: General knowledge was good in the urban area (53.2%) and poor in the rural (35.3%) a percentage difference of 17.9% (P 0.05) and advising them on ways to avoid spreading cough (urban 73.5%, rural 83.3%, P>0.05) were common among the PMV. Conclusion: Urban PMV in Enugu state are more knowledgeable about tuberculosis than rural PMV, but their practices in relation to the disease are at par with harmful aspects. They need to be trained to improve their capacity for quality interaction with tuberculosis suspects. Key words: Patent medicine vendor, tuberculosis, control
The Transition of Junior Occupational Therapists to Clinical Practice: Report of a Preliminary Study

This study investigated the feasibility of identifying the factors that influenced occupational therapists' decision to practise, or cease to practise, within one year of qualification. Pre-qualification and post-qualification questionnaires completed by the study respondents (n=177) explored a selection of variables, both personal and employment-related. Outcome measures included turnover and withdrawal from practice (attrition). The study identified a number of factors of potential influence, concluding that a larger study to explore these factors was both feasible and timely.
Alcohol, diabetes and the student body

Abstract Type 1 diabetes is one of the most common chronic conditions of adolescence and young adulthood. It is characterized by a demanding and complex management regime. Individuals with diabetes must engage in continual self-care actions if they are to minimize their risks of developing long-term diabetes' complications. Research has demonstrated, however, that many young adults experience difficulties engaging in these types of practice, and indeed often engage in practices that are risky for their diabetes control. Narrative approaches could provide important insights into the reasons why young people engage in risky activities. In this article, the narratives of a particular group of young adults with Type 1 diabetes (university students) are examined to see what social and spatial factors underlie their risky alcohol consumption practices. This paper contributes to geographical research on risk and chronic illness, and to understandings of the interactions between bodies, spaces and risk.
Flow pattern similarities in the near wake of three bird species suggest a common role for unsteady aerodynamic effects in lift generation

Analysis of the aerodynamics of flapping wings has yielded a general understanding of how birds generate lift and thrust during flight. However, the role of unsteady aerodynamics in avian flight due to the flapping motion still holds open questions in respect to performance and efficiency. We studied the flight of three distinctive bird species: western sandpiper (Calidris mauri), European starling (Sturnus vulgaris) and American robin (Turdus migratorius) using long-duration, time-resolved particle image velocimetry, to better characterize and advance our understanding of how birds use unsteady flow features to enhance their aerodynamic performances during flapping flight. We show that during transitions between downstroke and upstroke phases of the wing cycle, the near wake-flow structures vary and generate unique sets of vortices. These structures appear as quadruple layers of concentrated vorticity aligned at an angle with respect to the horizon (named ‘double branch’). They occur where the circulation gradient changes sign, which implies that the forces exerted by the flapping wings of birds are modified during the transition phases. The flow patterns are similar in (non-dimensional) size and magnitude for the different birds suggesting that there are common mechanisms operating during flapping flight across species. These flow patterns occur at the same phase where drag reduction of about 5% per cycle and lift enhancement were observed in our prior studies. We propose that these flow structures should be considered in wake flow models that seek to account for the contribution of unsteady flow to lift and drag.
Iron status and C-reactive protein in canine leishmaniasis.

OBJECTIVE To investigate the iron status, its relationship with C-reactive protein and the prognostic value of both in canine leishmaniasis. METHOD Eighty-six dogs with leishmaniasis and two control groups (healthy dogs and dogs with diseases other than leishmaniasis) were selected. Iron status indicators and C-reactive protein were compared between the three groups. Correlations between C-reactive protein and iron, ferritin and total iron-binding capacity were evaluated in dogs with leishmaniasis. Iron, total iron-binding capacity and ferritin were compared between dogs stratified according to similar C-reactive protein concentrations. The mortality rate at 30 days post-diagnosis was compared between groups. Iron status indicators and C-reactive protein were compared between survivors and non-survivors. RESULTS Dogs with leishmaniasis had lower iron and total iron-binding capacity and higher ferritin and C-reactive protein. There was a significant but low correlation of C-reactive protein with iron, ferritin and total iron-binding capacity. Dogs with leishmaniasis had decreased iron and total iron-binding capacity and increased ferritin compared to other ill patients with similar C-reactive protein concentrations. Mortality was not significantly different between groups but non-survivor dogs with leishmaniasis had higher C-reactive protein and lower total iron-binding capacity. CLINICAL SIGNIFICANCE Inflammation contributes to the iron status alterations found in canine leishmaniasis but other mechanisms are likely involved. Low total iron-binding capacity and increased C-reactive protein are risk factors for outcome in canine leishmaniasis.
Interactional leader–follower sensorimotor communication strategies during repetitive joint actions

Non-verbal communication is the basis of animal interactions. In dyadic leader–follower interactions, leaders master the ability to carve their motor behaviour in order to ‘signal’ their future actions and internal plans while these signals influence the behaviour of follower partners, who automatically tend to imitate the leader even in complementary interactions. Despite their usefulness, signalling and imitation have a biomechanical cost, and it is unclear how this cost–benefits trade-off is managed during repetitive dyadic interactions that present learnable regularities. We studied signalling and imitation dynamics (indexed by movement kinematics) in pairs of leaders and followers during a repetitive, rule-based, joint action. Trial-by-trial Bayesian model comparison was used to evaluate the relation between signalling, imitation and pair performance. The different models incorporate different hypotheses concerning the factors (past interactions versus online movements) influencing the leader's signalling (or follower's imitation) kinematics. This approach showed that (i) leaders' signalling strategy improves future couple performance, (ii) leaders used the history of past interactions to shape their signalling, (iii) followers' imitative behaviour is more strongly affected by the online movement of the leader. This study elucidates the ways online sensorimotor communication help individuals align their task representations and ultimately improves joint action performance.
Inter- and intraobserver agreement in interpretation of CT features of medial coronoid process disease.

OBJECTIVES To evaluate inter- and intraobserver reliability of the assessment of computed tomography features commonly used in the identification and classification of medial coronoid process disease and to assess inter- and intraobserver variability in the identification of the percentage ulna sclerosis from single transverse computed tomography images. METHODS Eight observers, on two occasions, reviewed 84 standardised single transverse computed tomography images acquired at the level of the apex of the medial coronoid process. Observers assessed: medial coronoid process disease, coronoid process fragmentation, osteophytes, sclerosis grade and sclerosis delineation with normal bone defined using a sclerometer. Cohen's kappa and intraclass correlation coefficient were calculated. RESULTS Inter-observer agreement was fair to moderate for identification of computed tomography changes consistent with medial coronoid process disease, moderate to almost perfect for fragmentation, and moderate for osteophyte detection. Agreement was poor for sclerosis grading. Percentage sclerosis measured with a sclerometer had moderate to almost perfect inter- and intraobserver agreement. CLINICAL SIGNIFICANCE These findings suggest that subjective assessment of computed tomography images is less repeatable than more quantitative methods.