Miracle-dz/UniFAR-FTUs huggingface dataset:
Stimulus-selective Inhibition of Rat Osteocalcin Promoter Induction and Protein-DNA Interactions by the Homeodomain Repressor Msx2*

Osteocalcin (OC) is a matrix calcium-binding protein expressed in osteoblasts and odontoblasts undergoing mineralization. OC expression is up-regulated in part by signals initiated by basic fibroblast growth factor (FGF2), cyclic AMP or forskolin (FSK), and calcitriol via defined elements and DNA-protein interactions in the OC promoter. We identified the OC gene as a target for transcriptional suppression by Msx2, a homeodomain transcription factor that controls ossification in the developing skull. In this study, we examine the effects of Msx2 expression on OC promoter activation (luciferase reporter) by FGF2/FSK and calcitriol in MC3T3-E1 osteoblasts. Expression of Msx2 decreases basal activity of the 1-kilobase (−1050 to +32) rat OC promoter by 80%; however, the promoter is still inducible 3-fold by calcitriol. By contrast, OC promoter induction by FGF2/FSK is completely abrogated by Msx2. Because intrinsic Msx2 DNA binding activity is not required for the Msx2 suppressor function, we assessed whether Msx2 represses OC activation by regulating DNA-protein interactions at the FGF2 response element (OCFRE) and compared these interactions with those occurring at the calcitriol response element (VDRE). Treatment of MC3T3-E1 cells with FGF2/FSK or calcitriol up-regulates specific DNA-protein interactions at the OCFRE or VDRE, respectively, as detected by gel shift assay. Preincubation of crude nuclear extracts with recombinant glutathioneS-transferase (GST)-Msx2 dose-dependently inhibits OCFRE DNA binding activity, whereas GST has no effect. Msx2 itself does not bind the OCFRE. Residues 132–148 required for Msx2 core suppressor function in transfection assays are also required to inhibit OCFRE DNA binding activity. By contrast, GST-Msx2 has no effect on calcitriol-regulated DNA-protein interactions at the VDRE. Using gel shift as an assay, the OCFRE DNA-binding protein OCFREB was purified to about 50% homogeneity from MG63 osteosarcoma cells. Recombinant Msx2 inhibits purified OCFREB DNA binding activity, whereas the Msx2 variant lacking residues 132–148 is inactive. Thus, Msx2 abrogates up-regulation of the OC promoter by FGF2/FSK in part by inhibiting OCFREB binding to the OCFRE.
Radical cystectomy for high risk patients with superficial bladder cancer in the era of orthotopic urinary reconstruction

Background. Many patients with aggressive clinically superficial bladder cancer ultimately die of their cancer, due in part to resistance to undergo radical cystectomy. Radical cystectomy is usually curative for patients with pathologic superficial tumors. Orthotopic urinary reconstruction has lessened the morbidity and life‐style changes in patients after cystectomy, and may increase patient acceptance of cystectomy as therapy for high risk superficial tumors.
STXBP4 Drives Tumor Growth and Is Associated with Poor Prognosis through PDGF Receptor Signaling in Lung Squamous Cell Carcinoma

Purpose: Expression of the ΔN isoform of p63 (ΔNp63) is a diagnostic marker highly specific for lung squamous cell carcinoma (SCC). We previously found that Syntaxin Binding Protein 4 (STXBP4) regulates ΔNp63 ubiquitination, suggesting that STXBP4 may also be an SCC biomarker. To address this issue, we investigated the role of STXBP4 expression in SCC biology and the impact of STXBP4 expression on SCC prognosis. Experimental Design: We carried out a clinicopathologic analysis of STXBP4 expression in 87 lung SCC patients. Whole transcriptome analysis using RNA-seq was performed in STXBP4-positive and STXBP4-negative tumors of lung SCC. Soft-agar assay and xenograft assay were performed using overexpressing or knockdown SCC cells. Results: Significantly higher levels of STXBP4 expression were correlated with accumulations of ΔNp63 in clinical lung SCC specimens (Spearman rank correlation ρ = 0.219). Notably, STXBP4-positive tumors correlated with three important clinical parameters: T factor (P < 0.001), disease stage (P = 0.030), and pleural involvement (P = 0.028). Whole transcriptome sequencing followed by pathway analysis indicated that STXBP4 is involved in functional gene networks that regulate cell growth, proliferation, cell death, and survival in cancer. Platelet-derived growth factor receptor alpha (PDGFRα) was a key downstream mediator of STXBP4 function. In line with this, shRNA mediated STXBP4 and PDGFRA knockdown suppressed tumor growth in soft-agar and xenograft assays. Conclusions: STXBP4 plays a crucial role in driving SCC growth and is an independent prognostic factor for predicting worse outcome in lung SCC. These data suggest that STXBP4 is a relevant therapeutic target for patients with lung SCC. Clin Cancer Res; 23(13); 3442–52. ©2017 AACR.
The overexpression of salivary cytokeratins as potential diagnostic biomarkers in head and neck squamous cell carcinomas

Background Cytokeratin (CK) intermediate filaments are demonstrated to have enormous potential in regulating cellular motility and cancer progression. There are more than 20 divergent CKs that have been identified, of which CK 8, 17, 18 and 19 are reported to be elevated in the tumour biopsies of head and neck cancer squamous cell carcinoma (HNSCC) patients. However, CK expression profiles in the saliva of HNSCC patients have not been investigated. We aim to investigate the mRNA expression profiles of CKs in saliva collected from healthy controls, HPV-negative and -positive HNSCC patients. Methods Oral rinse samples were collected from 42 cancer-free healthy controls (age-matched) and patients who have been diagnosed with HPV-negative (n = 20) and -positive (n = 48) HNSCC. Results Here, we report that the mRNA expression profiles of CKs differed in saliva collected from healthy controls and HNSCC patients. The mRNA expression levels of CK 8 and 18 were significantly elevated in saliva collected from HPV-negative HNSCC patients; whilst, CK 17 and 19 were expressed at a higher mRNA level in saliva collected from HPV-positive HNSCC patients compared to healthy controls. Importantly, receiver operating characteristic (ROC) analysis showed salivary CK 8 and 18 to have superior sensitivity and specificity in discriminating the HPV-negative HNSCC patients from healthy controls (80% and 86%) as well as between HPV-negative and -positive HNSCC patients (75% and 81%). Conclusion In summary, we have demonstrated that an aberrant expression of salivary CKs may serve as a potential non-invasive diagnostic biomarker in HNSCC.
RESPONS PERTUMBUHAN DAN HASIL TIGA VARIETAS SORGUM (Sorghum bicolor (L.) Moench) RATOON I TERHADAP APLIKASI BAHAN ORGANIK TANAMAN SORGUM PERTAMA

Penelitian ini bertujuan untuk mengetahui pengaruh dosis bahan organik yang diaplikasikan pada tanaman sorgum pertama terhadap pertumbuhan dan hasil tanaman sorgum ratoon I, pertumbuhan dan hasil tanaman sorgum ratoon I yang terbaik pada tiga varietas yang dicoba, dan mengetahui pengaruh interaksi antara dosis bahan organik yang diaplikasikan pada tanaman sorgum pertama dengan tiga varietas yang dicoba terhadap pertumbuhan dan hasil tanaman sorgum ratoon I. Penelitian ini dilaksanakan di Balai Pengkajian Teknologi Pertanian (BPTP) Kebun Percobaan Natar, Desa Negara Ratu, Kecamatan Natar, Kabupaten Lampung Selatan dan Laboratorium Ilmu Tanaman, Fakultas Pertanian, Universitas Lampung yang dilaksanakan pada bulan September sampai dengan Desember 2013. Perlakuan disusun secara faktorial dengan Rancangan Petak Terbagi (Split Plot Design) dalam Rancangan Kelompok Teracak Sempurna (RKTS) dengan tiga ulangan. Petak percobaanyang digunakan pada penelitian ini berukuran 4m x 4 m. Tiap satu satuan percobaan seluas 16 m dengan jarak tanam 80x20 cm. Hasil penelitian menunjukkan bahwa ; Perlakuan dosis bahan organik 15 ton/ha menghasilkan bobot biji/malai sorgum ratoon I tertinggi yaitu 45,64 g/tanaman atau setara 285,25 g/m2 2; Varietas Numbu menunjukkan hasil bobot biji/ malai sorgum ratoon I tertinggi yaitu 55,37 g/tanaman atau setara 346,06 g/m sedangkan, varietas Wray menghasilkan bobot brangkasan basah tertinggi, yaitu 0,54 kg/tanaman atau setara 3,37 kg/m2 2 ; dan Kombinasi antara dosis bahan organik dan varietas sorgum yang tepat untuk menghasilkan bobot brangkasan basah tertinggi adalah dosis bahan organik 5 ton/ha dengan varietas Wray yaitu 0,54 kg/tanaman atau setara 3,37 g/m2.
Spatial Dependence in Sovereign Wealth Funds' Investments

The aim of this paper is to identify the driving forces of Sovereign wealth funds' investments. For this, we develop an original econometric framework that quantifies the role of spatial dependence in the location of investments, and uses the Inverse Hyperbolic Sine transformation of the dependent variable in a spatial panel model context. This transformation copes with two features of net flows, namely an highly skewed distribution and the presence of zero and negative values. Using a large-scale database, we provide evidence of negative spatial dependence, investments in one country being on average at the expense of its neighbors.
Effects of an adapted physical activity program on the physical condition of elderly women: an analysis of efficiency.

BACKGROUND Specific research tools and designs can assist in identifying the efficiency of physical activity in elderly women. OBJECTIVES To identify the effects of physical activity on the physical condition of older women. METHOD A one-year-long physical activity program (123 sessions) was implemented for women aged 60 years or older. Four physical assessments were conducted, in which weight, height, BMI, blood pressure, heart rate, absences, grip strength, flexibility, VO2max, and static and dynamic balance were assessed. The statistical analyses included a repeated measures analysis, both inferential (analysis of variance--ANOVA) and effect size (Cohen's d coefficient), as well as identification of the participants' efficiency (Data Envelopment Analysis--DEA). RESULTS Despite the observation of differences that depended on the analysis used, the results were successful in the sense that they showed that physical activity adapted to older women can effectively change the decline in physical ability associated with aging, depending on the purpose of the study. The 60-65 yrs group was the most capable of converting physical activity into health benefits in both the short and long term. The >65 yrs group took less advantage of physical activity. CONCLUSION Adherence to the program and actual time spent on each type of exercise are the factors that determine which population can benefit from physical activity programs. The DEA allows the assessment of the results related to time spent on physical activity in terms of health concerns. Article registered in Clinicaltrials.gov under number NCT01558401.
The Cognitive Estimation Test (CET): Psychometric limitations in neurorehabilitation populations

The Cognitive Estimation Test (CET), which purportedly assesses aspects of executive functioning, consists of answering questions that require deductive reasoning. This paper explored the CET's psychometric properties in neurorehabilitation samples, including its reliability and strength of association with tests involving varying levels of executive skill. Among 112 patients (age = 65.3 years, SD = 12.5) with various neurological impairments, CET performance had limited reliability and was moderately correlated with nearly all cognitive tests examined, regardless of their executive demands. These findings highlight the poor divergent validity of the CET and raise questions of its utility in cognitive assessment.
E±ciency and the Disposition E®ect in NFL Prediction Markets ¤

Examining NFL betting contracts at Tradesports.com, we nd mispricing consistent with the disposition e®ect, where investors are more likely to close out pro table positions than losing positions. Prices are too low when teams are ahead and too high when teams are behind. Returns following news events exhibit short-term reversals and longer-term momentum. These results do not appear driven by liquidity or nonnancial reasons for trade. Finding the disposition e®ect in a negative expected return gambling market questions standard explanations for the e®ect (belief in mean reversion, prospect theory). It is consistent with cognitive dissonance, and models with time-inconsistent behavior.
Antidepressant monotherapy for bipolar type II major depression.

OBJECTIVES Bipolar type II (BP II) disorder is thought to be distinct from BP I disorder on genetic and biological grounds, and it is not merely a milder form of the illness. It affects 1.5-2.5% of the US adult population, and is characterized by highly recurrent depressive episodes with a substantial morbidity from alcoholism and non-affective psychopathology, and a higher suicide rate than either BP I or unipolar depression. Treatment recommendations for BP II depression are based upon concerns over drug-induced manic-switch episodes, and suggest using either a mood stabilizer alone or a combination of an SSRI plus a mood stabilizer. Recent evidence, however, indicates that the rate of manic switch episodes may be modest in BP II patients. Recent studies have provided evidence that antidepressant monotherapy may be an effective initial and long-term treatment for BP II major depression with a low manic-switch rate. METHODS In this article, we review the recent literature on BP II disorder, with a focus on the treatment of BP II major depression. RESULTS We present a summary of data from recent studies by our group and others indicating that antidepressant monotherapy for BP II depression may be safe and effective with a low manic-switch rate. CONCLUSION Antidepressant monotherapy may be beneficial for some patients with BP II major depression.
Yes, ‘it’ did happen again – the Minsky crisis in Asia

During his lifetime Hyman Minsky made a seminal contribution to the development of financial Keynesianism. In this book, leading academics celebrate his work and explore his economic legacy. Special attention is paid to his work on contemporary economic method, the Great Depression, the European single currency and the global financial system and recent banking and financial crises – in particular the crisis in Asia. An attempt is made to categorise Minsky's brand of post Keynesianism and to compare his work with the Keynesian and Marxian traditions.
Validation of a food frequency questionnaire to determine vitamin D intakes using the method of triads.

BACKGROUND Dietary sources of vitamin D (both natural and fortified) are increasingly contributing to consumers' vitamin D intake and status. Therefore, the present study aimed to validate a vitamin D food frequency questionnaire (FFQ) for the assessment of habitual vitamin D intake. METHODS A total of 49 apparently healthy consenting adults (aged 18-64 years) from the local community were sampled at the end of winter. Dietary intakes were recorded using a 4-day weighed food record (4d-WFR) and a 17-item FFQ based on foods known to contribute to dietary vitamin D intake. Fasting vitamin D status was quantified by serum 25-hydroxyvitamin D [25(OH)D] using liquid chromatography tandem mass spectrometry. The method of triads was applied using these three measurements to determine the overall validity of the FFQ. RESULTS Vitamin D intakes from 4d-WFR ranged between 0.42 and 31.65 μg day(-1), whereas intakes determined from the FFQ ranged from 1.03 to 36.08 μg day(-1). Serum 25(OH)D concentrations ranged between 12.89 and 279.00 nmol L(-1). The mean (SD) difference between the FFQ and 4d-WFR was +1.62 ( 3.86). There were strong correlations between the vitamin D intake estimated by the FFQ and that from the 4d-WFR (r = 0.562) and also with serum 25(OH)D concentrations (r = 0.567). Vitamin D intake estimated from the 4d-WFR was also strongly correlated with serum 25(OH)D concentrations (r = 0.411). The overall validity coefficient calculated using the method of triads was high (0.881). CONCLUSIONS The vitamin D FFQ has been validated for use in future studies aiming to assess habitual vitamin D intake.
Social and economic consequences of the ageing of Western European populations

Summary It is clear that the proportion of old people in Western Europe will increase considerably in the future. In this paper the author considers some of the effects which this process is likely to have on Western Society. As the old must be provided for by social security schemes, which are ultimately financed by the working population, the burden on the producer is likely to increase and may become so heavy that it will be resented and evaded. Some possible remedies are discussed, including postponement of the retiring age, and the immigration of workers from Mediterranean countries and possibly from outside Europe. The future of migration movements in Britain is also considered.
Performance Financeira Corporativa e Performance Social Corporativa: Desenvolvimento Metodológico e Contribuição Teórica dos Estudos Empíricos* Corporate Financial Performance and Corporate Social Performance: Methodological Development and the Theoretical Contribution of Empirical Studies

One of the roles of accounting is to provide information on business performance, either through financial accounting indicators or otherwise. Theoretical-empirical studies on the relationship between Corporate Financial Performance (CFP) and Corporate Social Performance (CSP) have increased in recent years, indicating the development of this research field. However, the contribution to the theory by empirical studies is made in an incremental manner, given that each study normally focuses on a particular aspect of the theory. Therefore, it is periodically necessary to conduct an analysis to evaluate how the aggregation of empirical studies has contributed to the evolution of the theory. Designing such an analysis was the objective of the present study. The theoretical framework covered the following: stakeholder theory, the relationship between CSP and CFP, good management theory, and slack resource theory. This research covered a 15-year period (1996 to 2010), and the data collection employed a search tool for the following databases: Ebsco, Proquest, and ISI. The sampling process obtained a set of 58 exclusively theoretical-empirical and quantitative articles that test the CSP-CFP relationship. The main results in the theoretical field reinforce the proposed positive relationship between CSP and CFP and good management theory and demonstrate a deficiency in the explanation of the temporal lag in the causal relationship between CSP and CFP as well as deficiencies in the description of the CSP construct. These results suggest future studies to research the temporal lag in the causal relationship between CSP and CFP and the possible reasons that the positive association between CSP and CFP has not been assumed in some empirical studies.
A1 adenosine receptor inhibition of cyclic AMP formation and radioligand binding in the guinea‐pig cerebral cortex

1 A1 adenosine receptors were investigated by radioligand binding and functional studies in slices and particulate preparations from guinea‐pig cerebral cortex. 2 Binding of the adenosine receptor antagonist radioligand, 8‐cyclopentyl‐[3H]‐1,3‐dipropylxanthine (DPCPX) to guinea‐pig cerebral cortical membranes exhibited high density (1410 ± 241 fmol mg−1 protein) and high affinity (Kd 3.8 ± 0.3 nm). 3 [3H]‐DPCPX binding to guinea‐pig cerebral cortical membranes was displaced in a monophasic manner by adenosine receptor antagonists with the rank order of affinity (Ki values, nm): DPCPX (6) < xanthine amine congener (XAC, 153) < PD 115,199 (308). 4 Agonist displacement of [3H]‐DPCPX binding was biphasic and exhibited the following rank order at the low affinity site (Ki values): 2‐chloro‐N6‐cyclopentyl‐adenosine (CCPA, 513 nm) = N6‐R‐phenyl‐isopropyladenosine (R‐PIA, 526 nm) = M6‐cyclopentyladenosine (CPA, 532 nm) < 2‐chloroadenosine (2CA, 3.2 μm) = 5′‐N‐ethylcarboxamidoadenosine (NECA, 4.6 μm) < N6‐S‐phenylisopropyladenosine (S‐PIA, 19.9 μm). 5 In cerebral cortical slices, [3H]‐DPCPX binding was displaced by antagonists and agonists in an apparently monophasic manner with the rank order of affinity (Ki values, nm): DPCPX (14) < XAC (45) < R‐PIA (266) < PD 115,199 (666) < S‐PIA (21000). 6 Cyclic AMP accumulation stimulated by 30 μm forskolin in guinea‐pig cerebral cortical slices was inhibited by R‐PIA, CCPA and CPA up to 1 μm in a concentration‐dependent fashion with IQo values of 14, 18, and 22 nm, respectively. All three analogues inhibited the forskolin response to a similar extent (82–93% inhibition). NECA, S‐PIA and 2CA failed to inhibit the forskolin response, but rather enhanced the accumulation of cyclic AMP at concentrations of 100 nm or greater, presumably through activation of A2b adenosine receptors coupled to stimulation of cyclic AMP accumulation in guinea‐pig cerebral cortical slices. 7 The inhibition of forskolin‐stimulated cyclic AMP accumulation by CPA was antagonized with the rank order of affinity (Ki values, nm): DPCPX (6) < XAC (52) < PD 115,199 (505). 8 Xanthine‐based antagonists inhibited the adenosine receptor augmentation of histamine‐induced phosphoinositide turnover in guinea‐pig cerebral cortical slices with the rank order of affinity (Ki, nm): DPCPX (12) = XAC (17) < PD 155,199 (640). 9 In summary, we observe a good correlation between antagonist affinity at A1 receptors defined by radioligand binding, inhibition of cyclic AMP generation or augmentation of histamine‐evoked phosphoinositide turnover in guinea‐pig cerebral cortex.
The New Deal, the GI Bill, and the Post-War Housing

After the collapse of housing markets during the Great Depression, the government played a large role shaping the future of the housing . nance, housing policy in the New Deal, as well as the development of the GI Bill for war veterans that had signi.cant importance for mortgage . nance and education. Soon after, the housing markets witness the largest boom in recent history. The objective in the paper is to quantify the contribution of government intervention in housing markets in the expansion in U.S. home ownership using an equilibrium model of tenure choice. In the model home buyers have access to a menu of mortgage choices to . nance the acquisition of the house. The government also provides special programs, consistent with the provisions of the GI Bill and the tax code. The parameterized model is consistent with key aggregate and distributional features in the U.S. in 1940. The model is capable of accounting for 95 percent of the boom in homeownership. The model suggests that government policies had a non-trivial effect in the housing boom. All these policies has positive effects in the demand for housing by reducing the relative cost. For example, introduction of the mortgage deduction can account for 13 percent of the increase in ownership. The asymmetric treatment of owner-occupied housing also had signi.cant effects in housing demand. The model suggests that 35 percent of ownership in that period can be accounted for this factor. Again, the results are in-line with Rosen and Rosen (1980) that predict 25 percent decline. The general equilibrium effects are important. Ignoring the income and price effects should reduce the incentives to own a home even more. When these adjustments are not available, the model predicts a 45 percent decline (from 63 to 53 percent) in ownership.