Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Diet and Type of Pregnancy on Transcriptional Expression of Selected Genes in Sheep Mammary Gland

Simple Summary An experiment was designed to determine the effect of diet and type of pregnancy on the mammary gland development, measured by the transcriptional expression of genes involved in angiogenesis and cell turnover/lactogenesis. To that end, twin and single-bearing ewes were fed naturalized pasture or red clover from day −45 pre-partum until day +60 post-partum, taking samples of mammary tissue at day −10, +30 and +60 post-partum. The results showed that the group of twin-bearing ewes fed red clover was the best combination to increase the expression of genes associated to angiogenesis and cell turnover/lactogenesis in the mammary gland. Abstract These trials were carried out to determine firstly the effect of diet and type of pregnancy on the transcriptional expression of genes involved in angiogenesis and cell turnover/lactogenesis inside the sheep mammary gland from late gestation to late lactation. Eighteen Ile de France sheep, 8 twin- and 10 single-bearing ewes were alloted into two groups according to their diet, either based on ad libitum naturalized pasture or red clover hay plus lupine from day −45 pre-partum until day +60 post-partum. Samples from diets and mammary glands were collected at day −10 pre partum (time 1), day +30 (time 2) and day +60 post-partum (time 3) and analyzed by qRT-PCR. Additionally, samples from longissimus dorsi muscle were taken from lambs twice, at weaning and 45 days later, to determine the effect of the maternal treatment with regard to diet and type of pregnancy, on the mRNA expression of genes involved in lipid metabolism. The data was processed using the lme4 package for R, and SPSS Statistics 23.0 for Windows®. The results showed that the group of twin-bearing ewes fed red clover showed a higher expression of genes involved in angiogenesis before lambing and in cell turnover/lactogenesis during late lactation, explained by a lamb survival mechanism to delay apoptosis as a way to keep a secretory cells population and boosted by the diet quality, assuring a longer milk production potential during late lactation. Regarding lambs, apparently the maternal diet would influence the transcriptional expression of lipogenic enzymes in the longissimus dorsi muscle after weaning, but further studies are necessary to validate these results. In summary, Twin-bearing ewes fed red clover performed best at increasing the expression of genes associated with angiogenesis and cell turnover/lactogenesis in the mammary gland.
Microbiota and Immune-Mediated Skin Diseases—An Overview

In recent years, increased attention has been paid to the relationship between microbiota and various diseases, especially immune-mediated diseases. Because conventional therapy for many autoimmune diseases is limited both in efficacy and safety, there is an increased interest in identifying nutraceuticals, particularly probiotics, able to modulate the microbiota and ameliorate these diseases. In this review, we analyzed the research focused on the role of gut microbiota and skin in immunity, their role in immune-mediated skin diseases (IMSDs), and the beneficial effect of probiotics in patients with this pathology. We selected articles published between 2009 and 2019 in PubMed and ScienceDirect that provided information regarding microbiota, IMSDs and the role of probiotics in these diseases. We included results from different types of studies including observational and interventional clinical trials or in vivo and in vitro experimental studies. Our results showed that probiotics have a beneficial effect in changing the microbiota of patients with IMSDs; they also influence disease progression. Further studies are needed to better understand the impact of new therapies on intestinal microbiota. It is also important to determine whether the microbiota of patients with autoimmune diseases can be manipulated in order to restore homeostasis of the microbiota.
The clinical and genetic characteristics of permanent neonatal diabetes (PNDM) in the state of Qatar

Neonatal diabetes mellitus (NDM) is a rare condition that occurs within the first six months of life. Permanent NDM (PNDM) is caused by mutations in specific genes that are known for their expression at early and/or late stages of pancreatic beta‐ cell development, and are either involved in beta‐cell survival, insulin processing, regulation, and release. The native population in Qatar continues to practice consanguineous marriages that lead to a high level of homozygosity. To our knowledge, there is no previous report on the genomics of NDM among the Qatari population. The aims of the current study are to identify patients with NDM diagnosed between 2001 and 2016, and examine their clinical and genetic characteristics.
An impaired glucagon-like peptide-1 response is associated with prediabetes in polycystic ovary syndrome with obesity

Objective Impaired glucose homeostasis in polycystic ovary syndrome (PCOS) is associated with obesity, age, and disease phenotype. This study aimed to investigate the glucagon-like peptide-1 (GLP-1) response in patients with obesity and PCOS with normal glucose tolerance (NGT) or prediabetes. Methods Twenty-six women with obesity and PCOS were included. Thirteen women had NGT and 13 had prediabetes. Serum glucose, insulin, and GLP-1 levels were measured during an oral glucose tolerance test. Beta-cell function and insulin resistance were determined. Results Women with prediabetes had significantly lower GLP-1 levels than did those with NGT after a glucose load. GLP-1 levels <3.02 pM at 120 minutes were associated with prediabetes. Women with prediabetes had a lower oral glucose insulin sensitivity (OGIS) index and greater amount of visceral adipose tissue than did those with NGT. Plasma GLP-1 levels at 120 minutes were correlated with visceral adiposity and the OGIS index. A change in GLP-1 levels was correlated with a family history of type 2 diabetes. Conclusion The GLP-1 response is lower in patients with obesity, PCOS, and prediabetes than in those with obesity, PCOS, and NGT. Further investigation of the GLP-1 response as a potential separate risk factor for prediabetes in PCOS is required.
Magnetic resonance imaging (MRI) reveals high cardiac ejection fractions in red-footed tortoises (Chelonoidis carbonarius)

ABSTRACT The ejection fraction of the trabeculated cardiac ventricle of reptiles has not previously been measured. Here, we used the gold standard clinical methodology – electrocardiogram-gated flow magnetic resonance imaging (MRI) – to validate stroke volume measurements and end diastolic ventricular blood volume. This produced an estimate of ejection fraction in our study species, the red footed tortoise Chelonoidis carbonarius (n=5), under isoflurane anaesthesia of 88±11%. After reduction of the prevailing right-to-left intraventricular shunt through the action of atropine, the ejection fraction was 96±6%. This methodology opens new avenues for studying the complex hearts of ectotherms, and validating hypotheses on the function of a more highly trabeculated heart than that of endotherms, which have lower ejection fractions. Summary: Electrocardiogram-gated MRI was used to visualize the cardiac cycle and blood volume movement in red footed tortoises without the need for surgery; the determined ejection fraction was over 80%.
Exploratory and agile behaviors with central dopaminergic activities in open field tests in Formosan wood mice (Apodemus semotus)

ABSTRACT Taiwan is a mountainous island, and nearly 75% of its lands are 1000 m above sea level. Formosan wood mice, Apodemus semotus, are endemic rodents and are broadly distributed at altitudes between 1400 and 3700 m in Taiwan. Interestingly, Formosan wood mice show similar locomotor activity in the laboratory as they do in the wild. Hence, we are interested in studying whether exploratory behaviors and central dopaminergic activity are changed in the open field test. We used male C57BL/6J mice as the control, comparing their behavioral responses in the open field, step-down inhibitory avoidance discrimination and novel object recognition tests with those of male Formosan wood mice. We also examined dopamine and its major metabolite 3,4-dihydroxyphenylacetic acid in the medial prefrontal cortex, striatum and nucleus accumbens. In open field tests, Formosan wood mice revealed higher levels of locomotion and exploration than C57BL/6J mice. Learning and memory performance in the novel object recognition test was similar in both Formosan wood mice and C57BL/6J mice, but more agile responses in the inhibitory avoidance discrimination task were found in Formosan wood mice. There was no difference in behavioral responses in the open field test between new second-generation Formosan wood mice and Formosan wood mice that were inbred for more than 10 generations. After repeated exposure to the open field test, high levels of locomotion and exploration as well as central dopaminergic activities were markedly persistent in Formosan wood mice, but these activities were significantly reduced in C57BL/6J mice. Diazepam (anxiolytic) treatment reduced the higher exploratory activity and central dopaminergic activities in Formosan wood mice, but this treatment had no effect in C57BL/6J mice. This study provides comparative findings, as two phylogenetically related species showed differences in behavioral responses. Summary: Comparative findings of two phylogenetically related species (Formosan wood mice and C57BL/6J mice) indicate differences in behavioral responses, especially in the locomotor and exploratory activities in an open field test.
Aging is associated with low thyroid state and organ specific sensitivity to thyroxine.

BACKGROUND Serum thyroid state in the elderly correlates with extended longevity. We hypothesized that age impacts systemic, but also organ-specific thyroid state and response to thyroxine (T4). METHODS Young (3 months) and old (23 months) male mice were analysed at baseline and after acute T4 challenge. Age effects on circulating thyroid stimulating hormone (TSH) and thyroid hormone (TH) concentrations, transcript expression in the pituitary and thyroid were compared to organ-specific response characterized by hepatic and cardiac content of TH and TH metabolites and expression of TH-target genes as well as hepatic deiodinase 1 activity. RESULTS Circulating TH concentrations and hepatic and cardiac TH content were lower in old vs. young mice. After injection with T4, conversion of T4 to triiodothyronine was decreased in old mice while TH transport in liver and heart was not affected. Organ-specific TH response was augmented in old mice in liver but not heart, indicating age and tissue-specific sensitivity to TH. Compensatory increase of thyroid stimulating hormone subunit beta expression in the pituitary and increased serum TSH concentrations, but reduced expression of thyroid differentiation markers were found in old mice. CONCLUSIONS We suggest that reduced activity of the aged thyroid is responsible for the systemic low TH state in old mice. Furthermore, divergent TH metabolism and tissue response in liver and heart occur after T4 treatment in an aged organism. These rodent data are in agreement with a much narrower window for T4 substitution in the elderly patients to avoid overtreatment.
Mothers' and fathers' observed interaction with preschoolers: Similarities and differences in parenting behavior in a well-resourced sample

Parenting behavior is a key factor in children’s socio-emotional development. However, little is known about similarities and differences in maternal and paternal parenting behavior, as most studies have focused on mothers. The present study investigated similarities and differences in mothers’ and fathers’ parenting behavior during observed free play with their preschool children, in a Danish well-resourced sample. We examined differences in mean scores and associations between mothers’ and fathers’ sensitivity, intrusiveness and limit-setting assessed with the Coding Interactive Behavior instrument. Additionally, we conducted confirmatory factor analysis to test the model-fit between the measurement model and parental data. Exploratory factor analysis was conducted to investigate if maternal and paternal factor structures replicated the three parenting constructs, and to explore if certain parenting behaviors seemed specifically related to either mothering or fathering. Participants included 52 mothers, 41 fathers and their 5-year old children. Similar mean scores were found for mothers and fathers on all parenting constructs. Maternal and paternal parenting behavior were not correlated. Confirmatory factor analysis revealed a poor model-fit. For both mothers and fathers, latent factors related to sensitivity, intrusiveness and limit-setting emerged, which indicated that the Coding Interactive Behavior instrument was suitable for assessment of both maternal and paternal sensitivity, intrusiveness and limit-setting. However, item loadings suggested that the instrument assessed maternal sensitivity more accurately than paternal sensitivity in our sample. Two additional factors were retrieved for fathers, i.e. paternal performance and challenging behavior, and paternal teaching behavior. This finding may suggest that additional parenting constructs need to be developed for researchers to be able to thoroughly investigate similarities and differences in mothers’ and fathers’ parenting behavior. Despite difference in factor structure, we did not identify behaviors solely related to mothering or to fathering.
Development and Preliminary Evaluation of a Visual Annotation Tool to Rapidly Collect Expert-Annotated Weight Errors in Pediatric Growth Charts

Patient weights can be entered incorrectly into electronic health record (EHR) systems. These weight errors can cause significant patient harm especially in pediatrics where weight-based dosing is pervasively used. Determining weight errors through manual chart reviews is impractical in busy clinics, and current EHR alerts are rudimentary. To address these issues, we seek to develop an advanced algorithm to detect weight errors using supervised machine learning techniques. The critical first step is to collect labelled weight errors for algorithm training. In this paper, we designed and preliminarily evaluated a visual annotation tool using Agile software development to achieve the goal of supporting the rapid collection of expert-annotated weight errors. The design was based on the fact that weight errors are infrequent and medical experts can easily spot potential errors. The results show positive user feedback and prepared us for the formal user-centered evaluation as the next step.
Perceived stress and self-efficacy are associated with diabetes self-management among adolescents with type 1 diabetes: A moderated mediation analysis.

AIMS The aims of this study were to: a) determine if self-efficacy mediates the relationship between perceived stress and diabetes self-management in adolescents with type 1 diabetes; and b) explore whether perceived stress moderated the self-efficacy and diabetes self-management relationship. DESIGN Non-experimental, descriptive correlational design, conducted from January- December 2016. METHODS Guided by the Adaptation to Diabetes framework, data on demographic and clinical characteristics, perceived stress, self-efficacy and diabetes self-management were collected. Descriptive analyses and regression analyses were generated by SPSS Version 22. Structural equation modeling was implemented with the MPlus program. RESULTS There was no direct effect of perceived stress on diabetes self-management; however, self-efficacy mediated the relationship between perceived stress and diabetes self-management. Adolescents who had high self-efficacy and low perceived stress demonstrated better diabetes care activities and diabetes communication than would be predicted from the main effects of self-efficacy and perceived stress alone. CONCLUSIONS Decreasing perceived stress and improving self-efficacy are important strategies to improve diabetes self-management in adolescents with type 1 diabetes. IMPACT Adolescents with type 1 diabetes experience considerable stress with daily self-management demands. This study highlights the mediating role of self-efficacy on perceived stress and diabetes self-management. Assessment of perceived stress and self-efficacy in self-management tasks in adolescents with T1D may help nurses individualize self-management education and support. Incorporating strategies to promote stress management and self-efficacy in diabetes education may also improve diabetes self-management. This article is protected by copyright. All rights reserved.
Phytochemical Analysis, Biochemical and Mineral Composition and GC-MS Profiling of Methanolic Extract of Chinese Arrowhead Sagittaria trifolia L. from Northeast China

Sagittaria trifolia is a medicinal foodstuff of China and East Asia belonging to the family Alismataceae. Samples of S. trifolia tubers were collected from Meihekow, Siping, Jilin, Harbin and Wuchang from Northeast China. The current study was aimed to evaluate the qualitative and quantitative analysis, antioxidant activity, biochemical analysis and chemical composition of different populations of S. trifolia. By using Folin–Ciocalteu, aluminium chloride colourimetric and 1,1-diphenyl-1-picrylhydrazyl (DPPH), total phenol and flavonoids content and antioxidant activity was analysed. Furthermore, chemical composition, biochemical analysis and mineral substances were also determined. The results showed the presence of flavonoids, phenols, saponins, tannins, glycosides and steroids except for alkaloids and terpenoids by qualitative analysis. Quantitative analysis revealed that highest total phenol, flavonoids content and antioxidant potential identified from Meihekow, i.e., 2.307 mg GAE/g, 12.263 mg QE/g and 77.373%, respectively. Gas chromatography-mass spectrometry results showed the presence of 40 chemical compounds corresponding to 99.44% of total extract that might be responsible for antioxidant properties. Mineral and biochemical analysis revealed the presence of calcium, magnesium, potassium, sodium, iron, copper, zinc and, carbohydrate, protein, fibre and fat contents, respectively. Interestingly, all S. trifolia populations collected from different locations possess similar composition. The dietary values, phytoconstituents, antioxidant activities and nutritional and curative chemical compounds of S. trifolia are beneficial for the nutritherapy of human beings.
Discovery of New Inhibitors of Transforming Growth Factor-Beta Type 1 Receptor by Utilizing Docking and Structure-Activity Relationship Analysis

The transforming growth factor-beta (TGF-β) plays an important role in pathological fibrosis and cancer transformation. Therefore, the inhibition of the TGF-β signaling pathway has therapeutic potential in the treatment of cancer. In this study, the binding modes between 47 molecules with a pyrrolotriazine-like backbone structure and transforming growth factor-beta type 1 receptor (TβR1) were simulated by molecular docking using Discovery Studio software, and their structure–activity relationships were analyzed. On the basis of the analysis of the binding modes of ligands in the active site and the structure–activity relationships, 29,254 new compounds were designed for virtual screening. According to the aforementioned analyses and Lipinski’s rule of five, five new compounds (CQMU1901–1905) with potential activity were screened through molecular docking. Among them, CQMU1905 is an attractive molecule composed of 5-fluorouracil (5-FU), 6-mercaptopurine (6-MP), and 5-azacytosine. Interestingly, 5-FU, 6-MP, and 5-azacytidine are often used as anti-metabolic agents in cancer treatment. Compared with existing compounds, CQMU1901–1905 can interact with target proteins more effectively and have good potential for modification, making them worthy of further study.
Upfront autologous hematopoietic stem cell transplantation consolidation for patients with aggressive B‐cell lymphomas in first remission in the rituximab era: A systematic review and meta‐analysis

The outcomes for patients with diffuse large B‐cell lymphoma (DLBCL) with adverse clinical prognostic factors such as a high age‐adjusted International Prognostic Index (aaIPI) are not optimal. In the current study, the authors performed a systematic review and meta‐analysis to assess the totality of evidence pertaining to the efficacy of autologous hematopoietic stem cell transplantation (auto‐HCT) consolidation for patients with DLBCL in first remission.
The artificial object detection and current velocity measurement using SAR ocean surface images

Due to the fact that water surface covers wide areas, remote sensing is the most appropriate way of getting information about ocean environment for vessel tracking, security purposes, ecological studies and others. Processing of synthetic aperture radar (SAR) images is extensively used for control and monitoring of the ocean surface. Image data can be acquired from Earth observation satellites, such as TerraSAR-X, ERS, and COSMO-SkyMed. Thus, SAR image processing can be used to solve many problems arising in this field of research. This paper discusses some of them including ship detection, oil pollution control and ocean currents mapping. Due to complexity of the problem several specialized algorithm are necessary to develop. The oil spill detection algorithm consists of the following main steps: image preprocessing, detection of dark areas, parameter extraction and classification. The ship detection algorithm consists of the following main steps: prescreening, land masking, image segmentation combined with parameter measurement, ship orientation estimation and object discrimination. The proposed approach to ocean currents mapping is based on Doppler's law. The results of computer modeling on real SAR images are presented. Based on these results it is concluded that the proposed approaches can be used in maritime applications.
Outpatient urinary tract infections in an era of virtual health care: trends from 2008 to 2017.

BACKGROUND Urinary tract infections (UTIs) occur commonly, but recent data on UTI rates are scarce. It is unknown how the growth of virtual health care delivery affects outpatient UTI management and trends in the United States. PATIENTS AND METHODS From January 1, 2008 to December 31, 2017, UTIs from outpatient settings (office, emergency, and virtual visits) were identified from electronic health records at Kaiser Permanente Southern California using multiple UTI definitions. Annual rates estimated by Poisson regression were stratified by sex, care setting, age, and race/ethnicity. Annual trends were estimated by linear or piecewise Poisson regression. RESULTS UTIs occurred in 1,065,955 individuals. Rates per 1000 person-years were 53.7 (95% CI: 50.6 to 57.0) by diagnosis code with antibiotic and 25.8 (95% CI: 24.7 to 26.9) by positive culture. Compared to office and emergency visits, UTIs were increasingly diagnosed in virtual visits, where rates by diagnosis code with antibiotic increased annually by 21.2% (95% CI: 16.5% to 26.2%) in females and 29.3% (95% CI: 23.7% to 35.3%) in males. Only 32% of virtual care diagnoses had a culture order. Overall, UTI rates were highest and increased the most in older adults. Rates were also higher in Hispanic and White females and Black and White males. CONCLUSION Outpatient UTI rates increased from 2008 to 2017, especially in virtual care and among older adults. Virtual care is important for expanding access to health services, but strategies are needed in all outpatient care settings to ensure accurate UTI diagnosis and reduce inappropriate antibiotic treatment.