Miracle-dz/UniFAR-FTUs huggingface dataset:
Pomphorhynchus laevis: the intestinal acanthocephalan as a lead sink for its fish host, chub (Leuciscus cephalus).

Aqueous lead exposure of chub (Leuciscus cephalus) experimentally infected with the parasite Pomphorhynchus laevis resulted in a rapid accumulation of this metal in the intestinal acanthocephalans, reaching concentrations which were significantly greater than in the host muscle, liver, and intestine and approximately 1000 times higher than the exposure con centration. Parasitized chub accumulated significantly less lead in their intestinal wall than their uninfected conspecifics (Mann-Whitney U test, P </= 0.05). From in vitro studies it was shown that lead uptake of P. laevis cystacanths clearly increases by adding 1% eel bile to a commercial RMPI-1640 medium containing 0.1 microg ml(-1) Pb(2+) compared to the controls, which were maintained in RMPI-1640 medium containing lead at the same concentration but without bile. It is therefore most likely that P. laevis in chub absorb bile-bound lead from the host intestine and thus reduce its reabsorption by the intestinal wall, thereby interrupting the hepatic-intestinal cycling of the metal. This is the first example of a beneficial impact of a parasite on its host.
Direct Comparison of Manganese Detoxification/Efflux Proteins and Molecular Characterization of ZnT10 Protein as a Manganese Transporter*

Manganese homeostasis involves coordinated regulation of specific proteins involved in manganese influx and efflux. However, the proteins that are involved in detoxification/efflux have not been completely resolved nor has the basis by which they select their metal substrate. Here, we compared six proteins, which were reported to be involved in manganese detoxification/efflux, by evaluating their ability to reduce manganese toxicity in chicken DT40 cells, finding that human ZnT10 (hZnT10) was the most significant contributor. A domain swapping and substitution analysis between hZnT10 and the zinc-specific transporter hZnT1 showed that residue Asn43, which corresponds to the His residue constituting the potential intramembranous zinc coordination site in other ZnT transporters, is necessary to impart hZnT10's unique manganese mobilization activity; residues Cys52 and Leu242 in transmembrane domains II and V play a subtler role in controlling the metal specificity of hZnT10. Interestingly, the His → Asn reversion mutant in hZnT1 conferred manganese transport activity and loss of zinc transport activity. These results provide important information about manganese detoxification/efflux mechanisms in vertebrate cells as well as the molecular characterization of hZnT10 as a manganese transporter.
Childhood obstructive sleep apnoea: Hypertension was not mentioned

EDITOR—An important effect is missing in the editorial on childhood obstructive sleep apnoea by Kotagal.1 The neuropsychological sequelae of this condition were mentioned, but another effect was not—namely, high blood pressure. This omission is not uncommon as shown by a report on the global burden of hypertension.2 Currently, there is good evidence—for example, …
Nonparametric Hemodynamic Deconvolution of fMRI Using Homomorphic Filtering

Functional magnetic resonance imaging (fMRI) is an indirect measure of neural activity which is modeled as a convolution of the latent neuronal response and the hemodynamic response function (HRF). Since the sources of HRF variability can be nonneural in nature, the measured fMRI signal does not faithfully represent underlying neural activity. Therefore, it is advantageous to deconvolve the HRF from the fMRI signal. However, since both latent neural activity and the voxel-specific HRF is unknown, the deconvolution must be blind. Existing blind deconvolution approaches employ highly parameterized models, and it is unclear whether these models have an over fitting problem. In order to address these issues, we 1) present a nonparametric deconvolution method based on homomorphic filtering to obtain the latent neuronal response from the fMRI signal and, 2) compare our approach to the best performing existing parametric model based on the estimation of the biophysical hemodynamic model using the Cubature Kalman Filter/Smoother. We hypothesized that if the results from nonparametric deconvolution closely resembled that obtained from parametric deconvolution, then the problem of over fitting during estimation in highly parameterized deconvolution models of fMRI could possibly be over stated. Both simulations and experimental results demonstrate support for our hypothesis since the estimated latent neural response from both parametric and nonparametric methods were highly correlated in the visual cortex. Further, simulations showed that both methods were effective in recovering the simulated ground truth of the latent neural response.
Parallel-Alleys and Distance-Alleys on Horopter Plane in the Dark

On each of three frontoparallel planes at distances of about 98, 186, and 276 cm from the subject, two series of light points running horizontally in the dark, one above the other, were constructed by six subjects: a P-alley in which the two series appear as two straight lines that are parallel, and a D-alley in which each pair of points appear equally separated in the vertical direction. Luneburg's mapping functions between visual space and physical space have been used to obtain estimates of K (curvature) and σ (a constant related to depth perception). In addition, ratios of perceptual distances were assessed by four out of the six subjects with eleven triplets of points on the alleys and values of K were estimated (and the fits of theoretical equations were tested) without the use of the mapping functions. In contrast to experiments with P-alleys and D-alleys on the horizontal plane, K was not unequivocally negative in either of the two experiments.
Conservation of Plasmid DNA Sequences in Coronatine-Producing Pathovars of Pseudomonas syringae

In Pseudomonas syringae pv. tomato PT23.2, plasmid pPT23A (101 kb) is involved in synthesis of the phytotoxin coronatine (C. L. Bender, D. K. Malvick, and R. E. Mitchell, J. Bacteriol. 171:807-812, 1989). The physical characterization of mutations that abolished coronatine production indicated that at least 30 kb of pPT23A DNA are required for toxin synthesis. In the present study, 32P-labeled DNA fragments from the 30-kb region of pPT23A hybridized to plasmid DNAs from several coronatine-producing pathovars of P. syringae under conditions of high stringency. These experiments indicated that this region of pPT23A was strongly conserved in large plasmids (90 to 105 kb) that reside in P. syringae pv. atropurpurea, glycinea, and morsprunorum. The functional significance of the observed homology was demonstrated in marker-exchange experiments in which Tn5-inactivated sequences from the 30-kb region of pPT23A were used to mutate coronatine synthesis genes in the three heterologous pathovars. Physical characterization of the Tn5 insertions generated by marker exchange indicated that genes controlling coronatine synthesis in P. syringae pv. atropurpurea 1304, glycinea 4180, and morsprunorum 567 and 3714 were located on the large indigenous plasmids where homology was originally detected. Therefore, coronatine biosynthesis genes are strongly conserved in the plasmid DNAs of four producing pathovars, despite their disparate origins (California, Japan, New Zealand, Great Britain, and Italy).
Prognostic significance of clinicopathological factors in early breast cancer: 20 years of follow-up in a single-center analysis

Background To quantify the effect of traditional prognostic factors [nodal status, estrogen-receptor (ER), progesterone-receptor (PR), human epidermal growth factor receptor 2 (HER2)] on long-term outcome of patients with early breast cancer (EBC), treated in clinical practice over a period of about twenty years. Results 1198 consecutive patients were identified. Median DFS (disease-free survival): ER+/PR±/HER2−, 165 months (mo) if node-negative (N0) and 114mo if node-positive (N+) (p < 0.001); triple-negative (TN), 109mo if N0 and 65mo if N+ (p 0.144); ER+/PR±/HER2+ in patients not-treated with adjuvant trastuzumab (T−), not reached if N0 and 114mo if N+ (p 0.297); ER+/PR±/HER2+ in patients treated with trastuzumab (T+), 95mo if N0 and 85mo if N+ (p 0.615); ER−/PR−/HER2+ T−, not reached if N0 and 26mo if N+ (p 0.279); ER−/PR−/HER2+ T+, not reached if N0 and 66mo if N+ (p 0.014). Median OS (overall survival): ER+/ PR±/HER2−, 166mo if N0 and 144mo if N+ (p 0.028); TN, 158mo if N0 and 96mo if N+ (p 0.384); ER+/PR±/HER2+ T−, not reached if N0 and 157mo if N+ (p 0.475), ER+/PR±/HER2+ T+, not reached if N0 and 106mo if N+ (p 0.436); ER−/PR−/HER2+ T−, not reached if N0 and 34mo if N+ (p 0.273); ER−/PR−/HER2+ T+, not reached neither if N0 nor if N+ (p 0.094). Materials and Methods Disease-free survival (DFS) and overall survival (OS) were evaluated according to tumor characteristics, based on information retrospectively retrieved from patients’ medical records. Conclusions Pathological tumor characteristics and nodal status still represent useful tools in treatment selection and follow-up decision making of EBC patients in clinical practice.
How and when team regulatory focus influences team innovation and member creativity

Purpose This study aims at testing how team regulatory focus may relate to individual creativity and team innovation and addressing the fit/misfit issue of team regulatory focus and team bureaucracy. Design/methodology/approach We collected data from 377 members and their leaders within 56 R&D teams in two Taiwan companies. Findings A team promotion focus was positively related, whereas a team prevention focus was negatively related, to both team innovation and member creativity through team perspective taking and employee information elaboration, respectively. Furthermore, team bureaucracy played a moderating role that suppressed the indirect relationship between team regulatory focus and creativity. Originality/value This is one of first studies to explore an underlying mechanism linking team regulatory focus and both team innovation and member creativity. We provide a more complete view of the creative and innovation implications of team-level self-regulation.
‘All cities are equal, but some are more equal than others’. Policy mobility and asymmetric relations in inter-urban networks for sustainability

ABSTRACT Knowledge sharing is deemed an important function of transnational municipal networks. However, in the literature on these organisations, a critical discussion of the implications of this process is scant. This article unpacks the mechanisms regulating learning, and examines the cognitive and relational dynamics of knowledge exchange within socio-ecological urban networks. By analysing the experience of a small group of European post-industrial second cities in socio-ecological urban networks, this article shows that network members exchange ideas and practices to tackle urban regeneration issues. The data suggest that, despite touted as a peer-to-peer practice, knowledge sharing reinforces asymmetrical relationships among network members, enabling the ‘soft domination’ of more advanced cities over less successful ones.
Adaptive Feedback Cancellation Using a Partitioned-Block Frequency-Domain Kalman Filter Approach With PEM-Based Signal Prewhitening

Adaptive filtering based feedback cancellation is a widespread approach to acoustic feedback control. However, traditional adaptive filtering algorithms have to be modified in order to work satisfactorily in a closed-loop scenario. In particular, the undesired signal correlation between the loudspeaker signal and the source signal in a closed-loop scenario is one of the major problems to address when using adaptive filters for feedback cancellation. Slow convergence speed and limited tracking capabilities are other important limitations to be considered. Additionally, computationally expensive algorithms as well as long delays should be avoided, for instance, in hearing aid applications, because of power constraints, important to extend battery life, and real-time implementations requirements, respectively. We present an algorithm combining good decorrelation properties, by means of the prediction-error method based signal prewhitening, fast convergence, good tracking behavior, and low computational complexity by means of the frequency-domain Kalman filter, and low delay by means of a partitioned-block implementation.
Deep Supervised Learning Using Local Errors

Error backpropagation is a highly effective mechanism for learning high-quality hierarchical features in deep networks. Updating the features or weights in one layer, however, requires waiting for the propagation of error signals from higher layers. Learning using delayed and non-local errors makes it hard to reconcile backpropagation with the learning mechanisms observed in biological neural networks as it requires the neurons to maintain a memory of the input long enough until the higher-layer errors arrive. In this paper, we propose an alternative learning mechanism where errors are generated locally in each layer using fixed, random auxiliary classifiers. Lower layers could thus be trained independently of higher layers and training could either proceed layer by layer, or simultaneously in all layers using local error information. We address biological plausibility concerns such as weight symmetry requirements and show that the proposed learning mechanism based on fixed, broad, and random tuning of each neuron to the classification categories outperforms the biologically-motivated feedback alignment learning technique on the CIFAR10 dataset, approaching the performance of standard backpropagation. Our approach highlights a potential biological mechanism for the supervised, or task-dependent, learning of feature hierarchies. In addition, we show that it is well suited for learning deep networks in custom hardware where it can drastically reduce memory traffic and data communication overheads. Code used to run all learning experiments is available under https://gitlab.com/hesham-mostafa/learning-using-local-erros.git.
Cleft palate repair and variations

Cleft palate affects almost every function of the face except vision. Today a child born with cleft palate with or without cleft lip should not be considered as unfortunate, because surgical repair of cleft palate has reached a highly satisfactory level. However for an average cleft surgeon palatoplasty remains an enigma. The surgery differs from centre to centre and surgeon to surgeon. However there is general agreement that palatoplasty (soft palate at least) should be performed between 6-12 months of age. Basically there are three groups of palatoplasty techniques. One is for hard palate repair, second for soft palate repair and the third based on the surgical schedule. Hard palate repair techniques are Veau-Wardill-Kilner V-Y, von Langenbeck, two-flap, Aleveolar extension palatoplasty, vomer flap, raw area free palatoplasty etc. The soft palate techniques are intravelar veloplasty, double opposing Z-plasty, radical muscle dissection, primary pharyngeal flap etc. And the protocol based techniques are Schweckendiek's, Malek's, whole in one, modified schedule with palatoplasty before lip repair etc. One should also know the effect of each technique on maxillofacial growth and speech. The ideal technique of palatoplasty is the one which gives perfect speech without affecting the maxillofacial growth and hearing. The techniques are still evolving because we are yet to design an ideal one. It is always good to know all the techniques and variations so that one can choose whichever gives the best result in one's hands. A large number of techniques are available in literature, and also every surgeon incorporates his own modification to make it a variation. However there are some basic techniques, which are described in details which are used in various centres. Some of the important variations are also described.
Fluoxetine inhibits inflammatory response and bone loss in a rat model of ligature-induced periodontitis.

BACKGROUND Fluoxetine, a selective serotonin reuptake inhibitor, has been found recently to possess anti-inflammatory properties. The present study investigates the effects of fluoxetine on inflammatory tissue destruction in a rat model of ligature-induced periodontal disease. METHODS Thirty male Wistar rats were randomly assigned into three groups (n = 10 animals per group): 1) control rats (without ligature); 2) rats with ligature + placebo (saline; oral gavage); and 3) rats with ligature + fluoxetine (20 mg/kg/day in saline; oral gavage). Histologic analyses were performed on the furcation region and mesial aspect of mandibular first molars of rats sacrificed at 15 days after ligature-induced periodontal disease. Reverse transcription-polymerase chain reaction and zymography were performed to analyze the mRNA expression of interleukin (IL)-1β, cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and inducible nitric oxide synthase and the MMP-9 activity, respectively, in gingival tissues samples. RESULTS Compared to the ligature + placebo group, alveolar bone loss was reduced in the fluoxetine group (P <0.05), and the amount of collagen fibers in the gingival tissue was maintained. Moreover, in gingival tissue sampled 3 days after ligature attachment, fluoxetine administration reduced IL-1β and COX-2 mRNA expression. Fluoxetine downregulated MMP-9 activity, without affecting MMP-9 mRNA expression induced by ligature, compared to the ligature + placebo group (P <0.05). These data suggest that fluoxetine suppressed proinflammatory responses, as well as proteolytic enzyme activity, induced by ligature. CONCLUSION In the present study, fluoxetine suppresses the inflammatory response and protects against periodontal bone resorption and destruction of collagen fibers, suggesting that fluoxetine can constitute a promising therapeutic approach for periodontal diseases.
Effectiveness of the water resources allocation institution in Tunisia

This paper examines the water allocation system and its effects on water use, users9 behavior and on the country9s achievement of desirable goals. It investigates efficiency, equity, and sustainability issues that arise under this allocation rule. The current allocation system functions according to a queuing system of priorities, where the urban allocation is found to be the most efficient and least risky allocation, while the ecological sector bears the highest degree of climate risk variability. The rule of priority is applied to crops as well; the non-strategic crops have the least efficient and riskiest allocation. The current system allows the country to secure drinking water supply, but it does not create sufficient incentives for entitlement holders that have priority to increase their water use efficiency, does not guarantee ecosystem health and integrity and does not equally distribute the risk among users. An institutional reform is especially relevant to improve water use performance in the agriculture sector and the country9s ability to manage drought. The nonpriority system that allows farmers to exchange their water-use entitlement might increase social welfare of water use.
Heavy overtime work and depressive disorder among male workers.

BACKGROUND The association between overtime and depression is unclear and very few studies have examined the association between heavy overtime work, i.e. working more than 60 h per week, and depression. AIMS To examine the association between heavy overtime work and the onset of depressive disorder among male workers. METHODS A 1-year follow-up cohort study of male workers in a manufacturing company in Japan, between 2008 and 2009. Working hours, depressive disorder, assessed by the Center for Epidemiologic Studies Depression (CES-D) Scale (score ≥16 points), and covariates were measured at baseline and at follow-up. Participants who had depressive disorder at baseline were excluded. RESULTS At follow-up, 1194 participants aged between 18 and 71 years were analysed. Multiple logistic regression analysis revealed that the odds ratio for the new onset of depressive disorder was 4.5 (95% CI 1.8-11.1) times higher for employees working >60 h per week than for those working ≤50 h per week, when adjusted for age, lifestyle factors, work-related characteristics and socio-demographic characteristics at baseline and working hours at follow-up. However, the correlation between working 50.1 to 60 h per week and depressive disorder was not significant. The trend test of depressive disorder among groups by working hours was significant (P < 0.01). CONCLUSIONS Heavy overtime work is a risk factor for the new onset of depressive disorder in this population of male workers. Working >60 h per week may be the cut-off to screen for high-risk groups who need preventive action against depressive disorder.
Interleukin‐1β induces osteopontin expression in pulmonary fibroblasts

Osteopontin is a multifunctional matricellular protein identified as one of the most upregulated genes in pulmonary fibrosis. Experimental animal models have identified early pro‐fibrotic cytokines as essential to the pathogenesis of inflammation‐induced pulmonary fibrosis. However, the principal sources of osteopontin in the fibroproliferative lung, and the factors responsible for its induction, have not been fully defined. We isolated primary rat lung fibroblasts in culture to examine the expression and regulation of lung fibroblast‐derived osteopontin. Our results demonstrate a potent and dramatic increase in osteopontin expression induced by interleukin‐1β (IL‐1β), whereas tumor necrosis factor‐α, transforming growth factor‐β, and angiotensin II had minimal effect. Stimulation with IL‐1β resulted in the secretion of soluble osteopontin protein. We found that osteopontin expression by IL‐1β was regulated via signaling primarily through the mitogen‐activated protein kinase member ERK1/2, partially by p38 MAPK, but not at all by JNK. Finally, the mechanism of IL‐1β increase in osteopontin mRNA requires de novo transcription and translation. In conclusion, we find that osteopontin is expressed by primary lung fibroblasts and is potently upregulated by the early inflammatory and pro‐fibrotic cytokine IL‐1β. Activated fibroblasts may be a significant source of osteopontin production during lung fibrogenesis. J. Cell. Biochem. © 2005 Wiley‐Liss, Inc.