Miracle-dz/UniFAR-FTUs huggingface dataset:
Phosphorylation-induced Conformational Ensemble Switching in an Intrinsically Disordered Cancer/Testis Antigen*

Background: PAGE4, an intrinsically disordered protein up-regulated in prostate cancer, binds to c-Jun and potentiates its transactivation. Results: The effects of phosphorylation on PAGE4 conformation, dynamics, and c-Jun binding were determined by NMR. Conclusion: Phosphorylation induces a more compact conformational ensemble, restricting access to the c-Jun binding site. Significance: This study may help to explain how phosphorylation of PAGE4 alters its binding to c-Jun. Prostate-associated gene 4 (PAGE4) is an intrinsically disordered cancer/testis antigen that is up-regulated in the fetal and diseased human prostate. Knocking down PAGE4 expression results in cell death, whereas its overexpression leads to a growth advantage of prostate cancer cells (Zeng, Y., He, Y., Yang, F., Mooney, S. M., Getzenberg, R. H., Orban, J., and Kulkarni, P. (2011) The cancer/testis antigen prostate-associated gene 4 (PAGE4) is a highly intrinsically disordered protein. J. Biol. Chem. 286, 13985–13994). Phosphorylation of PAGE4 at Thr-51 is critical for potentiating c-Jun transactivation, an important factor in controlling cell growth, apoptosis, and stress response. Using NMR spectroscopy, we show that the PAGE4 polypeptide chain has local and long-range conformational preferences that are perturbed by site-specific phosphorylation at Thr-51. The population of transient turn-like structures increases upon phosphorylation in an ∼20-residue acidic region centered on Thr-51. This central region therefore becomes more compact and more negatively charged, with increasing intramolecular contacts to basic sequence motifs near the N and C termini. Although flexibility is decreased in the central region of phospho-PAGE4, the polypeptide chain remains highly dynamic overall. PAGE4 utilizes a transient helical structure adjacent to the central acidic region to bind c-Jun with low affinity in vitro. The binding interaction is attenuated by phosphorylation at Thr-51, most likely because of masking the effects of the more compact phosphorylated state. Therefore, phosphorylation of PAGE4 leads to conformational shifts in the dynamic ensemble, with large functional consequences. The changes in the structural ensemble induced by posttranslational modifications are similar conceptually to the conformational switching events seen in some marginally stable (“metamorphic”) folded proteins in response to mutation or environmental triggers.
Maximum likelihood detection for differential unitary space-time modulation with carrier frequency offset

Can conventional differential unitary space time modulation (DUSTM) be applied when there is an unknown carrier frequency offset (CFO)? This paper answers this question affirmatively and derives the necessary maximum likelihood (ML) detection rule. The asymptotic performance of the proposed ML rule is analyzed, leading to a code design criterion for DUSTM by using the modified diversity product. The resulting proposed decision rule is a new differential modulation scheme in both the temporal and spatial domains. Two sub-optimal multiple-symbol decision rules with improved performance are also proposed. For the efficient implementation of these, we derive a modified bound intersection detector (BID), a generalization of the previously derived optimal BID for the conventional DUSTM. The simulation results show that the proposed differential modulation scheme is more robust against CFO drifting than the existing double temporal differential modulation.
Identifying Reasons for Employee Turnover in Housekeeping Department-A Study of Selected Hotels in Delhi

Hotel industry in India is witnessing a number of challenges at domestic & global level. These challenges are faced due to number of reasons, which include government policies, competition, market size and human resource related issues. Over the time, the Human Resource related issues have emerged as big challenge for Indian hotel industry. Though there are many challenges but employee turnover is one of the major challenge that the hospitality industry facing. Deloiltte research team conducted a Comparison Trends Survey (2013-14)}, which shows that the average rate of employee turnover is 13% across the country in hospitality. According to the report of National Skill Development Corporation (NSDC )(2011)has projected human resource requirement in hotel industry in India will be 27, 72000 by the year 2022. Housekeeping department is considered to be the backbone of hotel operations. Further, the review of same report suggest that the share of housekeeping department in total H.R requirement will be 6,34000 by the year 2022, which is highest among all the other departments of hotel. New Delhi, being an administrative capital of India is always a “melting pot of diverse culture”, it is a city with a rich historical past & a world class international airport makes it act as a “Gateway to India”. Today it has wide variety of hotels & many more are coming. A report published by Confederation of India Industry (CII) (2012) shows that Delhi alone has 288 registered hotels, which account 12% of total registered hotels within the country ranging from budget to five star. Despite of remarkable growth in hotel sector, dealing with Human Resource issues is a problem. The present study focuses on employee turnover issues in housekeeping departments of hotels located in New Delhi & tries to find out various reasons of employee turnover.
AFLP analysis of type strains and laboratory and industrial strains of Saccharomyces sensu stricto and its application to phenetic clustering

Using nine primer pairs, amplified fragment length polymorphism (AFLP) analysis was conducted to characterize industrial, laboratory and type strains of Saccharomyces sensu stricto. S. cerevisiae, S. bayanus, S. carlsbergensis and S. paradoxus had species‐specific AFLP profiles, with some variations among the strains. Nineteen wine, ale, bakery, whisky and laboratory strains of S. cerevisiae were differentiated by two primer pairs, while out of 19 strains of sake yeast, two groups consisting of two and eight strains were not differentiated using nine primer pairs. A phenogram of 41 strains of S. cerevisiae, two strains of S. bayanus, the type strain of S. pastorianus, three strains of S. carlsbergensis, one hybrid strain of S. cerevisiae and S. bayanus and the type strain of S. paradoxus was obtained by the unweighted pair group method, using arithmetic averages (UPGMA) based on the percentage of shared AFLP fragments of each sample pair. This phenogram demonstrated clear separations of S. cerevisiae, S. bayanus, S. carlsbergensis and S. paradoxus. However, S. pastorianus ATCC 12752T showed the highest percentages of shared fragments with the strains of S. bayanus, and formed a cluster with them. Except for the type strain of S. pastorianus, the percentages of shared fragments showed a similar tendency with reported data of DNA relatedness. The cluster of S. cerevisiae separated into three subclusters: one consisting of sake and shochu strains and a whisky strain; another consisting of bakery, wine, ale and whisky strains; and a third consisting of laboratory strains. Copyright © 2001 John Wiley & Sons, Ltd.
Means or meaning: the logic of Paul’s rhetoric in Galatians 3:10-14

Gal. 3:10-14 is still one of the most controversial and challenging passages in Paul's letters. The logic of Paul's rhetoric is that which mainly baffles. Study of this text has been hampered by an inadequate appreciation of the ranges of possible meanings, at all semantic levels. We seek to redress this lack m chapter 2. We survey the science of logic. We discover overlooked semantic possibilities for three key word-groups in Paul's rhetoric. and could be "discourse" lexical concepts. By Paul very possibly intends "accomplishments" rather than "endeavour. " Chapter 3 finds the indicated senses Paul’s. Effectively multiplying our data via sociolinguistic cognizance that identical words may denote different "realities" for speaker and hearer, we discover that Paul's usage implies a three-fold working semantic hypothesis: For Paul "faith" believes in a covenantal condition besides itself, namely obedience (endeavour to fulfil God's commands); Paul is basically denying that justification depends upon any particular amount of accomplishment of God’s commands; and the issue Paul is addressing is not that of die true means of justification, but that of the true meaning of ("righteousness" and thereby of) "justification" in the context of God’s covenant. The remainder of the thesis confirms and elaborates this overall meaning for Gal. 3:10-14. In verse 10 Paul points out that logically those who hold to the theory of "justification" have circumstances which contradict that theory; thus he is arguing by a "circumstantial" ad hominem type of argument. In verses 11-12 he circumstantially undermines his opponents' "accomplishments" righteousness-criterion by its incompatibility with Hab. 2:4. In verses 13-14, the "rescue" works entirely by causa cognoscendi: it is not a means of propitiation or repayment, either for man or for God. Our findings support our hypothesis.
Pharmacokinetic and bioequivalence evaluation of single‐tablet and separate‐tablet regimens for once‐daily cobicistat‐boosted elvitegravir in healthy Japanese male subjects: A randomized, two‐way crossover study

This randomized, two‐way crossover study evaluated the bioavailability of elvitegravir administered as the new individual tablet containing 150 mg and a cobicistat 150 mg tablet, concomitantly with a fixed‐dose combination tablet containing 200 mg of emtricitabine and 300 mg of tenofovir disoproxil fumarate (EVG + COBI + FTC/TDF), in comparison with a single‐tablet regimen containing the same dose of each component (EVG/COBI/FTC/TDF). Twenty‐four healthy Japanese male subjects received the two different elvitegravir treatments, the separate‐tablet or single‐tablet regimen, once‐daily for 10 days in each. The pharmacokinetic parameters (Cmax, AUCtau, and Ctau) of elvitegravir were investigated at Day 10 after each treatment, together with safety and tolerability. Relative to EVG/COBI/FTC/TDF, the geometric least‐squares mean ratios (GMR) and 90% confidence intervals (CIs) for elvitegravir Cmax and AUCtau were within the boundary of 0.8–1.25, while the upper limit of the 90% CI of GMR for Ctau was narrowly below the lack of bioequivalence boundary (0.79). No deaths, serious AEs, or drug‐related AEs occurred. In conclusion, Cmax and AUCtau of elvitegravir met the strict definition of bioequivalence, indicating that the two regimens were essentially bioequivalent. Treatment with both regimens for 10 days appeared to be safe and well tolerated.
Theorising a Quaker View of the Atonement

Gustaf Aulen described a classical view of the atonement, in which God through Christ triumphs over the forces of evil: ‘Christus victor’. Denny Weaver’s narrative Christus victor developed this view into a fully developed theory, spelled out as biblical narrative. A biblical theology framework provides a context for integrating this theory with Larry Shelton’s theory that God in the atonement establishes and maintains a new covenant community with humanity. Christus victor/covenant atonement incorporates biblical values of nonviolence and restorative justice; satisfaction and substitutionary theories are rooted in retributive justice and violence. George Fox was committed to a Christus victor view of Christ’s atonement. Lamb’s war writings of Fox, Burrough, and Nayler gave a powerful and original extension of that view. These Friends foreshadowed the combination of narrative Christus victor theory with covenant theory of the atonement.
‘Would you eat cultured meat?’: Consumers’ reactions and attitude formation in Belgium, Portugal and the UK

NOTICE: this is the author’s version of a work that was accepted for publication in Meat Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Verbeke, W. , Marcu, A. , Rutsaert, P., Gaspar, R. , Seibt, B. , Fletcher, D. , & Barnett, J. (2015). 'Would you eat cultured meat? ': consumers' reactions and attitude formation in Belgium, Portugal and the United Kingdom. Meat Science, 102, 49-58. 10.1016/j.meatsci. 2014.11. 013
Chaos Behavior in the Discrete BVP oscillator

The discrete BVP oscillator obtained through the Euler method is investigated, and also first proved that there exist chaotic phenomena in the sense of Marotto's definition of chaos and two-period cycles. And numerical simulations not only show the consistence with the theoretical analysis but also exhibit the complex dynamical behaviors, including the ten-periodic orbit, a cascade of period-doubling bifurcation, quasiperiodic orbits and the chaotic orbits in Marotto's chaos and intermitten's chaos. The computations of Lyapunov exponents confirm the existence of dynamical behaviors.
Contribution of glutamate receptors to brain-derived neurotrophic factor-induced elevation of intracellular Ca2+levels

In the present study, acute effects of brain-derived neurotrophic factor (BDNF) on intracellular calcium concentration ([Ca2+]i) in cultured visual cortical neurons were investigated. We examined specifically whether pharmacological blockade of glutamatergic receptors interfered with effects of BDNF on [Ca2+]i. We found that blockade of N-methyl-D-aspartate (NMDA) receptors significantly reduced the number of BDNF-responsive neurons, while inhibition of metabotropic glutamate receptors (mGluR) completely prevented the effect of BDNF. By contrast, blockade of &agr;-amino-3-hydroxy-5-methyl-4-isoxasole propionic acid (AMPA) receptors did not affect the BDNF-induced increase of [Ca2+]i. Our results thus suggest that glutamate-mediated excitatory pathways are involved in the BDNF-induced rise of [Ca2+]i in visual cortical neurons, and that both mGluR and NMDA receptors play a critical role in this event.
Identification of healthy elderly fallers and non-fallers by gait analysis under dual-task conditions

Objective: We compared falling and non-falling healthy elderly subjects to identify balance disorders associated with falling. Gait parameters were determined when carrying out single and dual tasks. Design: Case comparison study. Setting: Subjects were studied in the gait laboratory at Hopital Roger Salengro, Lille, France. Subjects: A group of 40 healthy elderly women were assigned to one of two groups according to their falling history: 21 fallers aged 70.4±6.4 years and 19 non-fallers aged 67.0±4.8 years. All subjects performed first a single leg balance test with two conditions (eyes open/closed). Then, gait parameters were analysed under single-task and dual motor-task conditions (walking with a glass of water in the hand). Main measures: Falls, number of times suspended foot touched the floor during the single leg balance test, cadence, speed, stride time, step time, single-support time, stride length and step length during walking under single- and dual-task conditions. Results: During the single leg balance test, fallers placed their feet on the floor three times more often than non-fallers under eyes open conditions (P < 0.05) and twice as often under eyes closed conditions (P < 0.05). In the single-task condition, no significant difference in gait parameters was reported between fallers and non fallers. There was a significant difference (P < 0.05) in the gait parameters (cadence, speed, stride and step time, single-support time) between fallers and non-fallers under dual-task conditions. Conclusions: Dual tasks perturb walking in fallers, who exhibit deteriorated static balance. Consequently, walking under dual-task conditions plus a single leg balance test could be helpful in detecting walking disorders and planning physiotherapy to prevent falls.
Engineering management education post GFC and copenhagen: Are we on the right track?

As world leaders around the globe navigate around the global financial crisis (GFC) and climate change agendas, aspiring engineering and engineering educators must rethink the enabling pathways for the personal, professional and educational development of professional engineering managers. A call for reform has exhibited in the early 21st century in form of numerous reports and literature around the globe. However, a revisit may now be required to ensure that the recommendations remain valid in the education of the next generation of engineering managers. The paper looks to the latest literature to review the findings of those pre GFC reports and highlights some of the existing engineering management and leadership programs around the world. It comments on the adequacy of those programs post GFC and provides a clearer picture of the key components and pathways of an aspiring engineering manager's learning journey. The issues that educators need address within course and program design are identified and discussed.
A method of handling virtual objects that incorporates the sense of touch

This study aims at the application of the virtual reality technique to model construction and simulation in industrial design and the arts. This paper discusses several problems in the interactive direct manipulation of the virtual object from the viewpoint of real space. The first point is to realize the representation system which can represent the motion parallax without experiencing the response delay. For this purpose, the reduction of the measurement time for the positions of both eyes is investigated, and the problem is solved by developing the both-eye position measurement method using position-sensitive devices (PSD). The second point is to realize the accurate and direct specification method of manipulation from the real space. The direct specification using the hand or the stylus in the real space makes it difficult to realize an accurate manipulation since the error between the real space and the virtual space coordinates is sensed visually in a direct way. For this problem, a stylus for virtual object manipulation was developed. It has a special structure wherein the grip is in the real space and the pointer is in the virtual space. By this elaboration, the visual error in the position relation between the pointer and the object to be handled can be eliminated. Then the real-time processing is investigated for the decision of contact between the stylus pointer and the virtual object to be manipulated, as well as for the generation of the tactile sensation. The real-time processing is realized by utilizing the feature of the virtual pointer stylus and the drawing mechanism.
Factors influencing innovation decision making in Portuguese firms

There is an undeniable positive effect of innovation for both firms and the economy, with particular regards to the financial performance of firms. However, there is an important role of the decision making process for the allocation of resources to finance the innovation process. The aim of this paper is to understand what factors explain the decision making process in innovation activities of Portuguese firms. This is an empirical study, based on the modern theoretical approaches, which has relied on five key aspects for innovation: barriers, sources, cooperation, funding; and the decision making process. Primary data was collected through surveys to firms that have applied for innovation programmes within the Portuguese innovation agency. Univariate and multivariate statistical techniques were used. Our results suggest that the factors that mostly influence the Portuguese firms' innovation decision-making processes are economical and financial (namely those related to profit increase and labour costs reduction).
Angulated Implants for Fabrication of Implant Supported Fixed Partial Denture in the Maxilla

Until recently, angled abutments have been the only solution to correcting the trajectory of the emergence profile of labially inclined implants in the maxilla. However, the clinical implications of angled abutments reveal several shortcomings. Newly designed angulated implants with a 12-degree restorative platform angulation are an alternative to angled abutments. The purpose of this article was to report a case utilizing new angulated implants (Co-axis, Keystone dental, Burlington, MA, USA) in the premaxilla thereby facilitating fabrication of a multi-unit implant retained fixed dental prosthesis.
Educating patients: Self-Management approaches

Purpose. To review and evaluate approaches to educating patients with fibromyalgia syndrome (FMS). Methods. A narrative literature review was undertaken to summarize the published literature on patient education for FMS patients. Results. A number of studies contain specific education strategies while others are combined with exercise or movement therapies or cognitive-behavioural therapy. Conclusions. Self-efficacy provides an effective theoretical model from which to understand how patients change as a result of education strategies that focus on self-management. Programmes that combine education with cognitive-behavioural techniques and exercise are most effective in enhancing self-efficacy and decreasing symptoms of FMS.
A pilot randomized clinical trial testing integrated 12‐Step facilitation (iTSF) treatment for adolescent substance use disorder

BACKGROUND AND AIMS The integration of 12-Step philosophy and practices is common in adolescent substance use disorder (SUD) treatment programs, particularly in North America. However, although numerous experimental studies have tested 12-Step facilitation (TSF) treatments among adults, no studies have tested TSF-specific treatments for adolescents. We tested the efficacy of a novel integrated TSF. DESIGN Explanatory, parallel-group, randomized clinical trial comparing 10 sessions of either motivational enhancement therapy/cognitive-behavioral therapy (MET/CBT; n = 30) or a novel integrated TSF (iTSF; n = 29), with follow-up assessments at 3, 6 and 9 months following treatment entry. SETTING Out-patient addiction clinic in the United States. PARTICIPANTS Adolescents [n = 59; mean age = 16.8 (1.7) years; range = 14-21; 27% female; 78% white]. INTERVENTION AND COMPARATOR The iTSF integrated 12-Step with motivational and cognitive-behavioral strategies, and was compared with state-of-the-art MET/CBT for SUD. MEASUREMENTS Primary outcome: percentage days abstinent (PDA); secondary outcomes: 12-Step attendance, substance-related consequences, longest period of abstinence, proportion abstinent/mostly abstinent, psychiatric symptoms. FINDINGS Primary outcome: PDA was not significantly different across treatments [b = 0.08, 95% confidence interval (CI) = -0.08 to 0.24, P = 0.33; Bayes' factor = 0.28). SECONDARY OUTCOMES during treatment, iTSF patients had substantially greater 12-Step attendance, but this advantage declined thereafter (b = -0.87; 95% CI = -1.67 to 0.07, P = 0.03). iTSF did show a significant advantage at all follow-up points for substance-related consequences (b = -0.42; 95% CI = -0.80 to -0.04, P < 0.05; effect size range d = 0.26-0.71). Other secondary outcomes did not differ significantly between treatments, but effect sizes tended to favor iTSF. Throughout the entire sample, greater 12-Step meeting attendance was associated significantly with longer abstinence during (r = 0.39, P = 0.008), and early following (r = 0.30, P = 0.049), treatment. CONCLUSION Compared with motivational enhancement therapy/cognitive-behavioral therapy (MET/CBT), in terms of abstinence, a novel integrated 12-Step facilitation treatment for adolescent substance use disorder (iTSF) showed no greater benefits, but showed benefits in terms of 12-Step attendance and consequences. Given widespread use of combinations of 12-Step, MET and CBT in adolescent community out-patient settings in North America, iTSF may provide an integrated evidence-based option that is compatible with existing practices.