Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibition of cyclin‐dependent kinases 2 and 4 activities as well as induction of cdk inhibitors p21 and p27 during growth arrest of human breast carcinoma cells by (−)‐epigallocatechin‐3‐gallate

(−)‐Epigallocatechin‐3‐gallate (EGCG) potently inhibits cell proliferation and suppresses tumor growth both in vitro and vivo, but little is known regarding the cell cycle regulatory proteins mediating these effects. This study investigated the effects of EGCG and other catechins on the cell cycle progression. DNA flow cytometric analysis indicated that 30 μM of EGCG blocked cell cycle progression at G1 phase in asynchronous MCF‐7 cells. In addition, cells exposed to 30 μM of EGCG remained in the G1 phase after release from aphidicolin block. Over a 24‐h exposure to EGCG, the Rb protein changed from hyper‐ to hypophosphorylated form and G1 arrest developed. The protein expression of cyclin D1, and E reduced slightly under the same conditions. Immunocomplex kinase experiments showed that EGCG inhibited the activities of cyclin‐dependent kinase 2 (Cdk2) and 4 (Cdk4) in a dose‐dependent manner in the cell‐free system. As the cells were exposed to EGCG (30 μM) over 24 h a gradual loss of both Cdk2 and Cdk4 kinase activities occurred. EGCG also induced the expression of the Cdk inhibitor p21 protein and this effect correlated with the increase in p53 levels. The level of p21 mRNA also increased under the same conditions. In addition, EGCG also increased the expression of the Cdk inhibitor p27 protein within 6 h after EGCG treatment. These results suggest that EGCG either exerts its growth‐inhibitory effects through modulation of the activities of several key G1 regulatory proteins such as Cdk2 and Cdk4 or mediates the induction of Cdk inhibitor p21 and p27. J. Cell. Biochem. 75:1–12, 1999. © 1999 Wiley‐Liss, Inc.
Clinical patterns of hand eczema in women

The hand eczema of 263 women was studied as 4 clinical patterns: palms and fingers, dorsa and lingers, lingers only and the entire hands. The palmar pattern was the commonest. Sensitisers affected the patterns equally but irritants were of less significance in the palmar group than among the rest. Atopics did not appear to have a distinctive pattern. Among the occupational group, irritants were of much greater importance than allergens.
Influence of three concentrations of DL-methionine or methionine hydroxy analog on milk yield and milk composition.

Sixty-eight Holstein cows were fed diets consisting of 60% concentrate and 40% forage, dry basis. Diets were supplemented with DL-methionine or methionine hydroxy analog at either 0, . 1, . 2, or . 3% of the concentrate. Dietary protein averaged 14% of total dry matter on all diets except for a positive control diet, which was 19% crude protein. Data were collected beginning 4 d postpartum and continued through 116 d postpartum. Milk yield was not influenced by either DL-methionine or methionine hydroxy analog. Supplementation with DL-methionine resulted in a quadratic response in milk fat percentage (3.34, 3.58, 3.72, 3.42) at 0, . 1, . 2, and . 3% of the concentrate, respectively. Milk fat percentage increased linearly (3.34, 3.64, 3.60, 3.73) with supplementation of methionine hydroxy analog at 0, . 1, . 2, and . 3%, respectively.
Terlipressin in Hepatorenal Syndrome

Objective: To compare the pharmacology, dosing, and adverse reactions of vasopressin and terlipressin for the treatment of hepatorenal syndrome (HRS) and assess the efficacy of the investigational drug terlipressin for HRS. Data Sources: Articles evaluating prospective studies for vasopressin and terlipressin were discussed after being identified through PubMed (1966-November 2010), International Pharmaceutical Abstracts (1970-November 2010), and EMBASE (1985-November 2010) with combinations of the following terms: vasopressin, terlipressin, and hepatorenal syndrome. In addition, reference citations from publications identified were reviewed. Thirteen studies were identified for terlipressin, along with 4 meta-analyses and 1 case report. For vasopressin, 2 studies were identified. Study Selection and Data Extraction: Prospective clinical studies directly comparing terlipressin and vasopressin were evaluated, as well as prospective clinical studies and meta-analyses for terlipressin in HRS. Data Synthesis: NO randomized, placebo-controlled trials using vasopressin for the treatment of type I HRS have been published, and 4 randomized studies Involving 197 patients provide the most current outcome data for use of terlipressin in HRS. Terlipressin differs significantly from vasopressin with regard to its pharmacology, dosing, and adverse drug reaction profile. There is a paucity of data on vasopressin for HRS. Conclusions: NO definitive recommendations can be made for the use of terlipressin for this indication until further, well-conducted studies are performed.
Pyranopterin Coordination Controls Molybdenum Electrochemistry in Escherichia coli Nitrate Reductase*

Background: The role of the pyranopterin component of the mononuclear molybdenum cofactor is largely unknown. Results: Variants of pyranopterin-coordinating amino acid residues were generated, and their effects on electrochemistry/catalysis investigated. Conclusion: The pyranopterin environment modulates molybdenum electrochemistry. Significance: The pyranopterin coordination environment enables redox-tuning of the molybdenum atom, and facilitates molybdoenzyme reactivity toward a broad range of substrates. We test the hypothesis that pyranopterin (PPT) coordination plays a critical role in defining molybdenum active site redox chemistry and reactivity in the mononuclear molybdoenzymes. The molybdenum atom of Escherichia coli nitrate reductase A (NarGHI) is coordinated by two PPT-dithiolene chelates that are defined as proximal and distal based on their proximity to a [4Fe-4S] cluster known as FS0. We examined variants of two sets of residues involved in PPT coordination: (i) those interacting directly or indirectly with the pyran oxygen of the bicyclic distal PPT (NarG-Ser719, NarG-His1163, and NarG-His1184); and (ii) those involved in bridging the two PPTs and stabilizing the oxidation state of the proximal PPT (NarG-His1092 and NarG-His1098). A S719A variant has essentially no effect on the overall Mo(VI/IV) reduction potential, whereas the H1163A and H1184A variants elicit large effects (ΔEm values of −88 and −36 mV, respectively). Ala variants of His1092 and His1098 also elicit large ΔEm values of −143 and −101 mV, respectively. An Arg variant of His1092 elicits a small ΔEm of +18 mV on the Mo(VI/IV) reduction potential. There is a linear correlation between the molybdenum Em value and both enzyme activity and the ability to support anaerobic respiratory growth on nitrate. These data support a non-innocent role for the PPT moieties in controlling active site metal redox chemistry and catalysis.
Shear Bond Strengths of Brackets Bonded with a New Self-Adhering Resin Composite

Purpose To determine the shear bond strength (SBS) of orthodontic brackets bonded with a self-adhering resin composite. Methods 45 freshly extracted mandibular incisors were randomly divided into 3 groups (n = 15 per group). Brackets were bonded with 3 bonding systems and cured with an LED light. Brackets were bonded with a new self-adhering resin cement in the study group. A conventional etch-and-rinse adhesive system and a self-etching adhesive system were used for comparison. After bonding, the SBS of the brackets were tested with a universal testing machine. Results Analysis of variance indicated a significant difference between groups (p<0.001). The highest values for SBS were measured in group I (21.80 ± 2.57 MPa). The SBS was significantly lower in groups II and III than in group I (P<.001). The lowest values for SBS were measured in group III (5.90 ± 0.90 MPa). SBS was significantly higher in group II than in group III (p<0.001). Conclusions The self-adhering adhesive system showed a clinically insufficient SBS, whereas the SBS of conventional etch-and-rinse adhesive system and the self-etching adhesive system were significantly higher and clinically sufficient.
Photochemistry of Bacteriochlorophylls in Human Blood Plasma: 1. Pigment Stability and Light‐induced Modifications of Lipoproteins

Transmetalated derivatives of bacteriochlorophyll are promising sensitizers in photodynamic therapy. Protocols using short delay times between injection and irradiation cause interest in the photochemistry of these pigments in the blood. Using near‐infrared irradiation where these pigments absorb strongly, we have studied the photochemistry of Zn‐ and Pd‐bacteriopheophorbide (WST09), and of the highly polar taurinated Pd‐derivative, WST11, in isolated fractions of human blood plasma. The stability of all pigments is increased in blood plasma, compared with monomeric solutions. Pd‐bacteriopheophorbide is much more stable than the other two derivatives. It also has a higher capacity for inducing reactive oxygen species, yet the consumption of oxygen is comparable. There is furthermore evidence for photobleaching under anoxic conditions. The generation of hydroperoxides (ROOH) is faster with Pd‐ than with Zn‐complexes; the formation of endoperoxides (ROOR′), measured as thiobarbituric acid reactive substances, is comparable with the two central metals. Formation of both ROOH and ROOR′ is increased in low‐density lipoproteins (LDL) compared with high‐density lipoproteins (HDL), which is probably related to the higher concentration of target molecules in the former. In HDL, extensive cross‐linking is induced among the apolipoproteins; judged from the electrophoretic mobility of LDL and HDL particles, there is also a gross structural change. Photosensitized cross‐linking is much less pronounced with high‐density proteins.
VAGINAL MICRONIZED NATURAL PROGESTERONE TREATMENT AND REDUCED RISK OF RECURRENT PRETERM BIRTH

Preterm birth (PTB) is defined as birth before 37 completed weeks of gestation, which occurs in approximately 10% of all pregnancies [1]. PTB causes about 70% of all neonatal deaths of nonanomalous infants, as well as neonatal morbidities including respiratory distress syndrome, intraventricular hemorrhage, necrotizing enterocolitis, bronchopulmonary dysplasia and long-term complications such as cerebral palsy [2,3]. The PTB rate has risen worldwide, and in South Korea, the PTB rate has also risen from 4.3% in 1995 to 10% in 2003 [1,4]. There are many known risk factors for PTB, but one of the most VAGINAL MICRONIZED NATURAL PROGESTERONE TREATMENT AND REDUCED RISK OF RECURRENT PRETERM BIRTH
Role Asymmetry and Code Transmission in Signaling Games: An Experimental and Computational Investigation

In signaling games, a sender has private access to a state of affairs and uses a signal to inform a receiver about that state. If no common association of signals and states is initially available, sender and receiver must coordinate to develop one. How do players divide coordination labor? We show experimentally that, if players switch roles at each communication round, coordination labor is shared. However, in games with fixed roles, coordination labor is divided: Receivers adjust their mappings more frequently, whereas senders maintain the initial code, which is transmitted to receivers and becomes the common code. In a series of computer simulations, player and role asymmetry as observed experimentally were accounted for by a model in which the receiver in the first signaling round has a higher chance of adjusting its code than its partner. From this basic division of labor among players, certain properties of role asymmetry, in particular correlations with game complexity, are seen to follow.
Prevalence and patterns of palatine and adenoid tonsilloliths in cone-beam computed tomography images of an Iranian population

Background: Tonsilloliths are calcified concretions that develop in tonsillar crypts. They are usually small and asymptomatic, so they are found accidentally during routine dental radiogrphy procedure. Large tonsilloliths can occur with clinical signs and symptoms. The purpose of this study was to evaluate the prevalence and patterns of palatine and adenoid tonsilloliths in cone-beam computed tomography (CBCT) images. Materials and Methods: In this cross-sectional study, 0.5-mm axial and coronal slices of 134 CBCT images were evaluated to determine the presence of palatine and adenoid calcifications. Their patterns such as being unilateral or bilateral as well as single or multiple and their largest linear sizes were reported. Results: Fifty-four (40.3%) patients with palatine tonsilloliths and 17 (12.7%) with adenoid calcifications were found. Thirty (55.6%) palatine tonsilloliths were unilateral, 19 (35.2%) were detected in the left tonsils. Approximately, 54 cases of 78 palatine calcifications were multiple. Seventeen patients had adenoid calcifications that 41.1% of them were unilateral. Fourteen adenoid calcifications were single. The mean ages of patients with palatine tonsilloliths and adenoid calcifications were 45.59 years and 46.53 years, respectively. The range of linear measurements of palatine tonsil calcifications was 0.9–4.2 mm (2.47-mm mean size) while adenoid calcifications ranged from 0.5 to 2.2 mm (0.95-mm mean size). The level of statistical significant difference was <0.05. Conclusion: Gender did not affect total prevalence, the pattern of tonsilar calcifications and their linear sizes. The prevalence of tonsilloliths increased with aging, but this variable did not have an effect on their linear size.
Free nilpotent minimum algebras

In the present paper we give a description of the free algebra over an arbitrary set of generators in the variety of nilpotent minimum algebras. Such description is given in terms of a weak Boolean product of directly indecomposable algebras over the Boolean space corresponding to the Boolean subalgebra of the free NM‐algebra. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
High sensitive C-reactive protein, adiponectin, and urine albumin excretion rate in Chinese coronary artery disease patients with different glucose tolerance status.

BACKGROUND Serum high sensitive C-reactive protein (hs-CRP), adiponectin levels and urine albumin excretion rate (UAER) are probably associated with inflammation and atherosclerosis. The aim of this study was to determine the three markers in coronary artery disease (CAD) subjects with different glucose tolerance status in a Chinese population and further explore the levels of the three markers in these subjects and the possible association of these markers with CAD risk factors and the severity of CAD as well. METHODS A total of 242 subjects with angiographically documented CAD were recruited, and then assigned to three groups: the normal glucose tolerance (NGT) + CAD group, including 100 CAD patients with NGT; the impaired glucose tolerance (IGT) + CAD group, 40 CAD patients with IGT; the type 2 diabetes mellitus (T2DM) + CAD group, 102 CAD patients with T2DM. Serum hs-CRP, adiponectin levels as well as UAER were measured in all subjects. RESULTS Serum hs-CRP levels were increased in the T2DM + CAD group compared with the NGT + CAD group (4.71 +/- 2.59) vs (3.60 +/- 2.46) mg/L, P = 0.037. Serum adiponectin levels were gradually decreased from the NGT + CAD to IGT + CAD to T2DM + CAD groups, (5.99 +/- 1.84), (5.82 +/- 1.72) and (4.65 +/- 1.71) mg/L, P = 0.002 and 0.040 for NGT + CAD and IGT + CAD groups vs T2DM + CAD group, respectively. While the UAER was gradually increased from the NGT + CAD to IGT + CAD to T2DM + CAD groups, (6.42 +/- 2.51), (6.89 +/- 2.94) and (15.03 +/- 4.22) microg/min (P < 0.001) for NGT + CAD and IGT + CAD groups vs T2DM + CAD group. Multiple linear stepwise regression analysis showed that waist-hip ratio (WHR) and low density lipoprotein cholesterol (LDL-C) were the significant determinants of serum hs-CRP levels; triglyceride (TG), high density lipoprotein cholesterol (HDL-C), age, WHR, T2DM, 2-hour serum insulin (2hINS), sex, and apolipoprotein B were the significant determinants of serum adiponectin levels; and systolic blood pressure (SBP), T2DM, and hemoglobin A1c (HbA1c) were the significant determinants of UAER in all subjects (R(2) = 0.070, 0.352, and 0.214, respectively). However, no significant correlation was seen for hs-CRP, adiponectin and UAER with the severity of CAD. Hs-CRP levels were significantly correlated with UAER. CONCLUSIONS There was a trend of increased serum hs-CRP levels from the NGT + CAD to IGT + CAD to T2DM + CAD groups, though it only showed significance in the T2DM + CAD group compared with the NGT + CAD group. Serum adiponectin levels were decreased and UAER was increased from the NGT + CAD to IGT + CAD to T2DM + CAD groups. Increased UAER and serum hs-CRP, and decreased adiponectin levels were associated with traditional CAD risk factors but failed to be correlated with the severity of CAD. Hs-CRP levels were significantly correlated with UAER.
Policy Gradient Adaptive Dynamic Programming for Data-Based Optimal Control

The model-free optimal control problem of general discrete-time nonlinear systems is considered in this paper, and a data-based policy gradient adaptive dynamic programming (PGADP) algorithm is developed to design an adaptive optimal controller method. By using offline and online data rather than the mathematical system model, the PGADP algorithm improves control policy with a gradient descent scheme. The convergence of the PGADP algorithm is proved by demonstrating that the constructed <inline-formula> <tex-math notation="LaTeX">${Q}$ </tex-math></inline-formula>-function sequence converges to the optimal <inline-formula> <tex-math notation="LaTeX">${Q}$ </tex-math></inline-formula>-function. Based on the PGADP algorithm, the adaptive control method is developed with an actor-critic structure and the method of weighted residuals. Its convergence properties are analyzed, where the approximate <inline-formula> <tex-math notation="LaTeX">${Q}$ </tex-math></inline-formula>-function converges to its optimum. Computer simulation results demonstrate the effectiveness of the PGADP-based adaptive control method.
Use of mesalazine slow release suppositories 1 g three times per week to maintain remission of ulcerative proctitis: a randomised double blind placebo controlled multicentre study

Background—Daily administration of rectal formulations of mesalazine is effective in preventing relapse of ulcerative proctitis. Maintenance of remission with lower doses would be an advantage. Aim—The efficacy of mesalazine suppositories (Pentasa) 1 g three times a week v placebo to maintain remission in patients with cryptogenetic proctitis was studied. Methods—Ninety five patients with cryptogenetic proctitis were randomised within two weeks of remission to receive for one year or until relapse three suppositories per week of either Pentasa (n=48) or placebo (n=47). In the case of a relapse, the patients received one suppository/day. Results—It was found that 25 of 48 subjectsv 18 of 47 remained in remission in the mesalazine and placebo groups respectively. The relapse rate was lower in the mesalazine group for the following time intervals: 0–90 days (19%v 38%, p=0.035), 0–180 days (29% v 54%, p=0.017), 0–270 days (38% v 60%, p=0.031), and 0–365 days (48% v 62%, p=0.18). Treatment of relapse with one suppository/day induced remission in 11 of 18 and 2 of 26 patients in the mesalazine and placebo groups respectively (p=0.001). Overall, 61%v 28% patients remained in the protocol and were in remission at one year (p=0.001). Tolerance was good. Conclusion—Mesalazine suppositories 1 g three times a week are effective for preventing relapses of cryptogenetic proctitis. Increasing the dose to 1 g/day is effective in a high proportion of subjects who relapsed.
Stroke prophylaxis in atrial fibrillation: who gets it and who does not? Report from the Stockholm Cohort-study on Atrial Fibrillation (SCAF-study).

AIMS Underuse of warfarin for stroke prophylaxis in atrial fibrillation (AF) is extensive and represents a major problem in clinical practice. To identify factors associated with warfarin treatment in eligible AF patients. METHODS AND RESULTS The study population consisted of all Swedish resident AF patients at the Stockholm South General Hospital during 2002 (n=2796). Medical records were examined and complemented by data from the Swedish National Hospital Discharge Register. Sixty-eight percent of the patients (1898/2796) had indications, and no apparent contraindications for warfarin treatment. Of these 54% (1029/1898) got warfarin. Factors favouring warfarin treatment after adjustment for other factors were history of ischaemic stroke, an implanted pacemaker, treatment in a cardiology rather than internal medicine ward and valvular defect. Factors associated with a reduced likelihood of warfarin treatment were paroxysmal type of AF and age >80 years. Important risk factors for stroke in AF like heart failure, hypertension, and diabetes did not increase the chances of warfarin treatment. CONCLUSION Risk stratification using known risk factors of stroke seems to affect warfarin treatment only to a minor degree in clinical practice. Undertreatment was particularly common in patients with paroxysmal AF and in patients aged >80 years and calls for improved clinical routines in accordance with international guidelines.
Action of C‐type natriuretic peptide (CNP) on passive avoidance learning in rats: involvement of transmitters

The action of C‐type of natriuretic peptide (CNP) was tested on one‐way passive avoidance learning in rats. The involvement of transmitters was investigated by pretreating the animals with different receptor blockers. CNP administered into the lateral brain ventricle caused a dose‐dependent facilitation of learning and consolidation of passive avoidance learning, but was ineffective on retrieval. Pretreatment of the animals with atropine, haloperidol or the nitric oxide synthase inhibitor nitro‐l‐arginine abolished the action of CNP. Phenoxybenzamine, naloxone, bicuculline, propranolol and methysergide were ineffective in modifying the action of CNP on consolidation. The results suggest that CNP is able to improve the learning and consolidation of learning in a passive avoidance paradigm, but is ineffective on retrieval processes. In the action of CNP, dopamine, acetylcholine and nitric oxide could be the mediating transmitters.
Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine

The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes—cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Betv1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.