Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of load manipulations, heart rate, and contractility on left ventricular apical rotation. An experimental study in anesthetized dogs.

BACKGROUND Left ventricular twist or torsion has been defined as the counterclockwise rotation of the ventricular apex with respect to the base during systole. We have recently shown that since base rotation is minimal, measurement of apex rotation reflects the dynamics of left ventricular (LV) twist. Since the mechanisms by which load and contractility affect twist are controversial, we aimed to determine the relation between apex rotation and volume, contractility, and heart rate under conditions in which dimensions and pressures were accurately measured. METHODS AND RESULTS Using our optical device coupled to the LV apex, apex rotation was recorded simultaneously with LV pressure, ECG, LV segment length, and minor-axis diameters (sonomicrometry) in 12 open-chest dogs. Using vena caval occlusion and volume loading, a linear end-diastolic (ED) relation between apex rotation and LV area index was obtained (slope, 0.61 +/- 0.06 degrees/percent change; intercept, -60.1 +/- 6.2 degrees; n = 10) that differed from the end-systolic (ES) relation (slope, 1.36 +/- 0.27 degree/percent change; intercept, -132.5 +/- 24.9 degrees; P < .005). With changes in contractility, afterload, or heart rate, for both ED and ES the apex rotation-volume points fell within the range of the relations established by changing preload, suggesting that volume is the major determinant of twist. Vena caval occlusion (preload and afterload decrease) caused an increase in amplitude of apex rotation, with maximal apex rotation occurring earlier in ejection. In contrast, acute volume loading (predominant preload increase) caused a small decrease in the amplitude of apex rotation, and twist relaxation was delayed into the isovolumic relaxation period. Likewise, with single-beat aortic occlusion (increased afterload), there was a slight decrease in the amplitude of apex rotation, and maximal apex rotation was delayed into the isovolumic relaxation period. Paired pacing (increased contractility) increased the total amplitude of apex rotation by 42% and caused a delay in untwisting until the end of the isovolumic relaxation period. An increase in heart rate over 150 beats per minute resulted in a significant decrease in the amplitude of apex rotation with a similar delay of twist relaxation into the isovolumic relaxation period. CONCLUSIONS The effects of load, contractility, and heart rate manipulations on LV twist as measured throughout the cardiac cycle by the optical apex rotation method are manifested by changes in both the amplitude and dynamics of torsion. LV twist at ED and ES is primarily a function of volume; this relation appears to be unaltered by heart rate, afterload, and contractility. Whereas decreased load caused early untwisting, increases in preload, afterload, heart rate, and contractility caused a consistent pattern of delay in twist relaxation.
The Education and Employment Status of Patients with Inflammatory Bowel Diseases

&NA; Inflammatory bowel disease (IBD) has the propensity to affect patients who are in their late teens and early 20s, an age when most people decide on their educational and career directions. This review describes the effects that IBD has on the continuum of education and employment. Patients with Crohn's disease and ulcerative colitis attain a similar level of education as that of the general population. The quality of life of such patients in school, as measured by both patients' and teachers' perceptions, indicates that, despite the difficulties that students face in terms of missed school time and physical inconveniences, teachers are generally perceived by students to have favorable attitudes toward helping them. Even though earlier work in the area of employment has suggested that the occurrence of IBD is clustered among people in white‐collar positions, recent data have suggested that certain environmental risks for IBD (i.e., sedentary or indoor jobs) may be associated with jobs classified as being white‐collar, and therefore having a white‐collar job may in itself not be a risk factor for the development of IBD. Patients with IBD have a higher rate of nonparticipation in the labor force, and the participation rate seems to maintain steady levels over time. A majority of patients with IBD continue in the same employment positions over a period of years. Patients with IBD, especially those who have undergone surgery, took more sick leave than their counterparts without IBD. A majority of patients with IBD favored the disclosure of their diagnosis to their employers and perceived little discrimination in the workplace. Furthermore, most employers were perceived by their employees with IBD as having fair attitudes toward the compensation provided for their employees with IBD.
Glial fibrillary acidic protein and βA4 protein deposits in temporal lobe of aging brain and senile dementia of the Alzheimer type: Relation with the cognitive state and with quantitative studies of senile plaques and neurofibrillary tangles

The aim of this study was to compare brain glial fibrillary acidic protein (GFAP) levels to the modifications of cognitive functions (Blessed test score [BTS]), the density of the main neuropathological lesions (senile plaques [SP] and neurofibrillary tangles [NFT]), and the density of the two main subtypes of βA4 deposits (classic plaques and diffuse deposits) in a series of patients with normal aging and senile dementia of the Alzheimer type of various degrees of severity. GFAP levels (enzymelinked immunosorbent assay [ELISA] technique) and the densities of changes were measured in the temporal lobe of 12 women over 75 years of age. Under these conditions, the ELISA assay could determine GFAP in brain homogenates (aqueous‐Triton buffer soluble extract) in a range from 2.5 ng to 600 ng per assay. Least affected patients (with a BTS of 19 and over) all ranged below 60 μg/mg protein. Most affected patients (with a BTS under 6) ranged above 150 μg/mg protein. However, interindividual variations were wide. A significant correlation between the BTS and the amount of GFAP could be found only when using the non parametric test of Spearman. There was a significant positive correlation between the amount of GFAP and the density of (1) SP, (2) NFT both revealed by Bodian's silver stain, and (3) classic βA4 plaques shown by immunocytochemistry. On the contrary, no correlation was observed with diffuse βA4 deposits. One case with very large amounts of diffuse βA4 deposits without SP or NFT showed no associated GFAP reactivity. This suggests that GFAP production is a critical event in the formation of classic SP.
The experiences of prenatal depression among Taiwanese women.

AIM This paper is a report of a study to describe Taiwanese women's experiences of prenatal depression. BACKGROUND Despite its long-term adverse effects for both mother and child, prenatal depression has received less scholarly attention than postpartum depression. Studies to date have proposed structured measures for quantifying prenatal depression, but the characteristics of this phenomenon have not been qualitatively analysed. METHOD A convenience sample of 12 Taiwanese pregnant women scoring above 15 on the Edinburgh Perinatal Depression Scale participated in in-depth interviews during 2005. The interviews were transcribed verbatim and analysed using content analysis. FINDINGS Unbalanced commitment to motherhood was the primary theme of the life experiences of prenatal depression in these Taiwanese women, which summarized their feelings of engagement and ambivalence towards their experiences of transition to motherhood. Five recurring sub-themes were identified: multiple conflicting roles, lack of social support, dissatisfaction with body image, future uncertainty and emotional instability. CONCLUSION Prenatal depression is a painful growth experience represented by silent and unbalanced commitment during the maternal role transition. Our findings may help healthcare professionals to understand the vulnerable experiences of pregnant women and devise preventive intervention strategies, such as prenatal education for both parents and comprehensive screening for prenatal depression in at-risk pregnant women, so that they are better prepared for the transition to motherhood.
Increased Resistance to Quinolones in Campylobacter jejuni: A Genetic Analysis of gyrA Gene Mutations in Quinolone‐Resistant Clinical Isolates

Campylobacter jejuni is a frequent cause of enteritis and sometimes it requires antimicrobial therapy. We have studied the evolution of resistance to nine antibiotics from 1990 to 1994 and investigated how frequently gyrA mutations are involved in the acquisition of quinolone resistance. The percentage of chloramphenicol‐, clindamycin‐, tertracycline‐ and amoxicillin plus clavulanic acid‐resistant strains has remained practically unchanged and erythromycin and gentamicin resistance has decreased, whereas the percentage of ampicillin‐, nalidixic acid‐ or ciprofloxacin‐resistant strains has almost doubled in the follow‐up period, from 56 to 76% for ampicillin‐ and from 47.5 to 88% for quinolone‐resistant strains. This study clearly shows that a mutation in Thr‐86 to Ile or Lys is a frequent mechanism associated with the acquisition of a high level of resistance to quinolones in clinical isolates of C. jejuni.
An upstream regulatory region mediates high-level, tissue-specific expression of the human alpha 1(I) collagen gene in transgenic mice

Studies in vitro have not adequately resolved the role of intronic and upstream elements in regulating expression of the alpha 1(I) collagen gene. To address this issue, we generated 12 separate lines of transgenic mice with alpha 1(I) collagen-human growth hormone (hGH) constructs containing different amounts of 5'-flanking sequence, with or without most of the first intron. Transgenes driven by 2.3 kb of alpha 1(I) 5'-flanking sequence, whether or not they contained the first intron, were expressed at a high level and in a tissue-specific manner in seven out of seven independent lines of transgenic mice. In most tissues, the transgene was expressed at levels approaching that of the endogenous alpha 1(I) gene and was regulated identically with the endogenous gene as animals aged. However, in lung, expression of the transgene was anomalously high, and in muscle, expression was lower than that of the endogenous gene, suggesting that in these tissues other regions of the gene may participate in directing appropriate expression. Five lines of mice were generated containing transgenes driven by 0.44 kb of alpha 1(I) 5'-flanking sequence (with or without the first intron), and expression was detected in four out of five of these lines. The level of expression of the 0.44-kb constructs in the major collagen-producing tissues was 15- to 500-fold lower than that observed with the longer 2.3-kb promoter. While transgenes containing the 0.44-kb promoter and the first intron retained a modest degree of tissue-specific expression, those without the first intron lacked tissue specificity and were poorly expressed in all tissues except lung. (ABSTRACT TRUNCATED AT 250 WORDS)
Translation of scales in cross-cultural research: issues and techniques.

AIMS This paper is a report of a study designed to: (i) describe issues and techniques of translation of standard measures for use in international research; (ii) identify a user-friendly and valid translation method when researchers have limited resources during translation procedure; and (iii) discuss translation issues using data from a pilot study as an example. BACKGROUND The process of translation is an important part of cross-cultural studies. Cross-cultural researchers are often confronted by the need to translate scales from one language to another and to do this with limited resources. METHOD The lessons learned from our experience in a pilot study are presented to underline the importance of using appropriate translation procedures. The issues of the back-translation method are discussed to identify strategies to ensure success when translating measures. FINDINGS A combined technique is an appropriate method to maintain the content equivalences between the original and translated instruments in international research. There are several possible combinations of translation techniques. However, there is no gold standard of translation techniques because the research environment (e.g. accessibility and availability of bilingual people) and the research questions are different. CONCLUSIONS It is important to use appropriate translation procedures and to employ a combined translation technique based on the research environment and questions.
The Impact of changes in stock prices and house prices on consumption

This paper,based on wealth effect theory,makes an empirical study for the wealth effect of our country's assets price.The main conclusions are as follows: both the stock market and real estate market show some wealth effects in China;the wealth effect in real estate market is far greater than that in the stock market;as the economy develops,the wealth effect of assets is strengthening.Meanwhile,there is an interacting effect between GDP and the social consumption;the development of the real market can promote national economy;but the effect of the stock market is limited.
Effect of ploughing and previous crop on winter wheat pests and their natural enemies under integrated farming system in Slovakia

The effects of ploughing and previous crop on winter wheat pests and their natural enemies under an integrated farming system was investigated in an experimental area in Slovakia. The experiment consisted of 4 small plots (50 m2 each) to which both organic and inorganic fertilisers were applied. Moreover, there was one application of herbicide and a fungicide on each plot. The ploughing was either deep or shallow, and either silage-maize or pea was used as a previous crop. The effects of the ploughing and previous crop was studied by means of multivariate redundancy analysis (RDA) and subsequent Monte-Carlo permutation test. The results showed that there was a considerable temporal variation in the abundance of insect pests and their natural enemies. Of the two husbandry treatments, only ploughing caused significant difference in the composition and abundance of both pests and natural enemies. However, while most of the pests favoured deep ploughing, the majority of natural enemies occurred more abundantly on shallowly ploughed plots. The previous crop did not affect numbers of either pests or natural enemies. Two systems, integrated and organic, which have been surveyed previously, were mutually compared with respect to the abundance of insect pests. The organic farming system turned out to be more sustainable.
CD4+ T Cells Mediate the Protective Effect of the Recombinant Asp f3-Based Anti-Aspergillosis Vaccine

ABSTRACT The mortality and morbidity caused by invasive aspergillosis present a major obstacle to the successful treatment of blood cancers with hematopoietic cell transplants. Patients who receive hematopoietic cell transplants are usually immunosuppressed for extended periods, and infection with the ubiquitous mold Aspergillus fumigatus is responsible for most cases of aspergillosis. Previously, we demonstrated that vaccination with recombinant forms of the A. fumigatus protein Asp f3 protected cortisone acetate-immunosuppressed mice from experimentally induced pulmonary aspergillosis. Here, we investigated the vaccine's protective mechanism and evaluated in particular the roles of antibodies and T cells. After vaccination, Asp f3-specific preinfection IgG titers did not significantly differ between surviving and nonsurviving mice, and passive transfer of anti-Asp f3 antibodies did not protect immunosuppressed recipients from aspergillosis. We experimentally confirmed Asp f3's predicted peroxisomal localization in A. fumigatus hyphae. We found that fungal Asp f3 is inaccessible to antibodies, unless both cell walls and membranes have been permeabilized. Antibody-induced depletion of CD4+ T cells reduced the survival of recombinant Asp f3 (rAsp f3)-vaccinated mice to nonimmune levels, and transplantation of purified CD4+ T cells from rAsp f3-vaccinated mice into nonimmunized recipients transferred antifungal protection. In addition, residues 60 to 79 and 75 to 94 of Asp f3 contain epitopes that induce proliferation of T cells from vaccinated survivors. Vaccine-primed CD4+ T cells are not expected to clear the fungal pathogen directly; however, they may locally activate immunosuppressed phagocytes that elicit the antifungal effect.
The Stability Curve of Bovine Adenosine Deaminase is Bimodal

Abstract We have determined the stability curve of bovine adenosine deaminase via titrations with guanidine hydrochloride at pH = 6.3 from 5 to 65° C. The data indicate that the enzyme undergoes an abrupt conformational transition at ∼29° C, a finding supported by a temperature scan of the intrinsic enzyme fluorescence emission. Analysis of the data above and below this temperature with the modified Gibbs-Helmholtz equation allows for complete description of the equilibrium unfolding thermodynamics for either enzyme conformation. The high-temperature form of the enzyme is described by ΔH° = 648 ± 37 kJ/mole, ΔCp = 23.2 ± 2.5 kJ/mole-K, and a heat denaturational temperature Th dn = 72.5 ± 0.9° C. The low-temperature form is described by ΔH° = 1284 ± 47 kJ/mole, ΔCp = 73.2 ± 4.9 kJ/mole-K, and Th dn = 32.6 ± 0.6° C. Further thermodynamic analysis of the conformations that predominate at 38.3° C, the bovine normal body temperature, and at 4° C, where the crystals for x-ray structural analyses were formed, suggest that the stability of either form is due to favorable amino acid side chain nonpolar interactions with these interactions being much more optimized in the low-temperature conformation. We therefore conclude that the structure as determined by x-ray crystallographic methods cannot be the physiological structure. The data also suggest that the general calculation of enzyme stability curves from the extrapolation of heat denaturation data may inaccurately represent the enzyme stability as a low- temperature, nondenaturational transition is assumed not to exist. Further consequences in terms of general enzyme catalysis are also discussed.
VALVULAR AND STRUCTURAL HEART DISEASES Original Studies Impact of Aortic Valve Calcification on the Outcome of Transcatheter Aortic Valve Implantation: Results from the Prospective Multicenter German TAVI Registry

Stephan Staubach,* MD, Jennifer Franke, MD, Ulrich Gerckens, MD, Gerhard Schuler, MD, Ralf Zahn, MD, Holger Eggebrecht, MD, Rainer Hambrecht, MD, Stefan Sack, MD, Gert Richardt, MD, Martin Horack, Jochen Senges, MD, Daniel H. Steinberg, MD, Jakob Ledwoch, Stephan Fichtlscherer, MD, Mirko Doss, MD, Nina Wunderlich, MD, and Horst Sievert, MD, on behalf of the German Transcatheter Aortic Valve Implantation—Registry Investigators
Functional impairment and disability in early rheumatoid arthritis--development over 5 years.

OBJECTIVE To evaluate the development of functional impairment and disability in early rheumatoid arthritis (RA). METHODS Sixty-three patients with definite RA with mean disease duration of about 1 year were followed for 5 years. Joint inflammation was evaluated with an active joint count, and radiographic changes in hands and feet with the Larsen method. Functional impairment of particular joint systems was assessed with a performance index, Signals of Functional Impairment Index, and disability with the Health Assessment Questionnaire (HAQ). RESULTS During the observation time the disease activity decreased, and the radiographic changes of hands and feet increased significantly. Joint replacement in 10 hips, 1 knee, and 1 shoulder were performed in 9 patients after median 43 months. At study start almost half the patients had impaired hand function, mostly affecting finger flexion and pincer grip. The most marked deterioration of joint function had occurred already after 2 years in metatarsophalangeal joints (55%), elbow joints (35%), ankle joints (30%), shoulder joints (28%), and hip joints (25%). The median HAQ level at study start was 0.8, and the median change of HAQ over 5 years was 0.1. (not significant). The progression of dysfunction was not linear but followed a highly variable course over the years. Patients with higher HAQ scores at study end could be correctly classified in 75% of the cases by the 3 factors, baseline HAQ score, female sex, and a low educational level. Presence of a replaced joint did not contribute significantly, but patients with joint replacement tended to be more disabled. CONCLUSION Functional outcome of RA after about 6 years of disease was fairly good. Functional impairment of different joints had progressed, but most patients were still mildly disabled. A subgroup of 9 patients had a worse disease course with rapidly progressing large joint destruction.
Recombinant activated factor VII in liver patients: a retrospective cohort study from Australia and New Zealand

Recombinant factor VIIa (rFVIIa) is used in the treatment of life-threatening haemorrhage that is refractory to conventional treatment. The evidence supporting this practice in patients with liver disease is very limited. It has been used as a salvage therapy in end-stage liver disease (ESLD), in orthotopic liver transplant (OLT), other surgery, and upper gastrointestinal bleeding (UGIB) subpopulations. It has also been used prior to procedures in patients with ESLD. Data were collected by the Australia and New Zealand Haemostasis Registry (ANZHR) to perform a retrospective cohort study on the different subgroups of liver patients. This included 115 cases of use of rFVIIa in liver patients from 20 hospitals. A retrospective cohort study on the different subgroups of liver patients was performed. Main outcome measures were reduction or cessation of bleeding and 28-day mortality. Variables previously shown to predict response to bleeding after administration of rFVIIa were examined to determine whether correlations exist. Salvage therapy with rFVIIa was associated with reduction or cessation in bleeding in 24 of 36 OLT patients, 24 of 36 UGIB patients and 15 of 26 of other surgery patients. Clinical response to rFVIIa in OLT patients and other surgery patients was associated with a significantly lower mortality compared to nonresponders (P = 0.003 and 0.022, respectively). There was no relationship between mortality and bleeding response in patients with UGIB. Variables including acidosis, hypothermia, hypofibrinogenaemia, thrombocytopenia and Model of End-Stage Liver Disease (MELD) score were not associated with clinical response to rFVIIa. Five cases of use prior to procedures are described. Recombinant FVIIa is used as rescue therapy in surgical patients with ESLD and refractory haemorrhage in Australia and New Zealand. Traditional haemostasis variables were not associated with clinical response to rFVIIa in this cohort. Response to rFVIIa is associated with decreased mortality in ESLD patients undergoing OLT and other surgery, but not in UGIB.