Miracle-dz/UniFAR-FTUs huggingface dataset:
Anti‐viral immune response of allogeneic irradiation bone marrow chimeras: cytotoxic T cell responsiveness depends upon H‐2 combination and infectious agent

Allogeneic irradiation bone marrow chimeras C57BL/10 (H‐2b) →B10.BR (H‐2k) and B10.BR (H‐2k)→C57BL/10 (H‐2b) were raised under specific pathogen‐free (SPF) conditions; they survived very well and were healthy under SPF for 3–4 months and subsequently, under conventional housing conditions, for 1 to 8 months. Their immune response against third‐party alloantigens was comparable with that of controls. Anti‐vaccinia virus responses were very low when compared with syngeneic control chimeras or unmanipulated control animals; if anti‐vaccinia virus cytotoxic T cell reactivity was measurable, it was specific for the bone marrow donor, rather than the recipient thymic H‐2 type. In contrast, the anti‐LCMV (lymphocytic‐choriomeningitis virus) response was excellent and comparable to that in controls for B10.BR (H‐2k)→C57BL/10 (H‐2b) chimeras, but was completely absent for C57BL/10 (H‐2b)→B10.BR (H‐2k) chimeras. LCMV‐specific cytotoxic effector T cells from B10.BR→C57BL/10 chimeras were restricted entirely to recipient H‐2b. In contrast to the asymmetric cytotoxic T cell response, both types of chimeras developed good primary footpad swelling reactions after local infection, which arose somewhat slower with LCMV than in control of chimeras. The capacity to control infection by Listeria monocytogenes was excellent for all controls and B10.BR→C57BL/10 chimeras but apparently absent in C57BL/10→B10.BR chimeras.
Análogos experimentais de seleção e extinção de metacontingências

A partir da explicacao do comportamento humano apresentada por Skinner em 1981, baseada no modelo de variacao e selecao por consequencia. E do conceito de metacontingencias proposto por Glenn, em 1986. Foram conduzidos 4 estudos experimentais, com objetivos de promover a selecao e a extincao de metacontingencias. O experimento 1 conseguiu demonstrar selecao de metacontingencias e dados que indicavam a extincao com a retirada da consequencia cultural selecionadora, mas ainda havia consequencias diferenciais durante a fase de extincao. Outros dois experimentos foram realizados no intuito de comparar os dados com uma fase de extincao sem qualquer consequencia diferencial. No experimento 2 nao foi obtido selecao e no experimento 3 houve selecao e extincao de contingencias comportamentais entrelacadas. O quarto experimento foi conduzido com objetivo de servir de grupo controle e deu sustentacao aos dados dos outros experimentos
A Pilot Study Assessing the Impact of Heat on the Transdermal Delivery of Testosterone

This study evaluated the effect of locally applied heat on the transdermal delivery of testosterone. Six healthy adult volunteers were tested three times while receiving a 5 mg androgen patch, the same patch with a heat‐generating patch, and no patch at all over 12 hours. Statistically significant differences in mean maximum serum testosterone concentration values were seen. Heat plus patch resulted in a mean maximum serum testosterone concentration of 939 ng/dl versus 635 ng/dl (patch only) and 425 ng/dl (no patch). (Heat + patch vs. no patch: p < 0.001; heat + patch vs. patch: p < 0.001; patch vs. no patch: p = 0.003.) The area under the curve of plasma testosterone concentration versus time values were means of 4114 ng/dl·h versus 1985 ng/dl·h for the patch‐only group (p = 0.001). The use of heat improved absorption of transdermal testosterone and decreased time to peak serum testosterone concentrations, resulting in a statistically significant difference in mean maximum serum testosterone concentrations compared with the use of the patch without heat.
Mesenchymal Stem Cell Manufacturing for Clinical Use

Mesenchymal stromal/stem cells (MSCs) are adult, self-renewable, multipotent cells that can be isolated in various tissues, most commonly bone marrow (BM) and adipose tissue. Because of their capacity for self-renewal and differentiation into tissues of mesodermal origin and due to their immunomodulatory ability, MSCs are used in many preclinical and clinical studies as possible new therapeutic agents for the treatment of a very broad range of conditions, including heart, hepatic, and neurodegenerative diseases. Whatever the tissue of origin or the clinical application, the MSCs must be expanded in vitro to obtain sufficient cell numbers, also when multiple doses are needed. The MSC manufacturing process for clinical use should comply with the principles of Good Manufacturing Practice (GMP). This ensures that cell preparations are produced and controlled, from the collection and manipulation of raw materials, through the processing of intermediate products, to the quality controls, storage, labelling and packaging, and release. The application of GMP to manufacture medicinal products such as MSCs must ensure that clinical trials are unaffected by inadequate safety, quality, or efficacy arising from unsatisfactory manipulation of the cells.
Peroxidasin: a novel enzyme‐matrix protein of Drosophila development.

Peroxidasin is a novel protein combining peroxidase and extracellular matrix motifs. Hemocytes differentiate early from head mesoderm, make peroxidasin and later phagocytose apoptotic cells. As hemocytes spread throughout the embryo, they synthesize extracellular matrix and peroxidasin, incorporating it into completed basement membranes. Cultured cells secrete peroxidasin; it occurs in larvae and adults. Each 1512 residue chain of the three‐armed, disulfide‐linked homotrimer combines a peroxidase domain with six leucine‐rich regions, four Ig loops, a thrombospondin/procollagen homology and an amphipathic alpha‐helix. The peroxidase domain is homologous with human myeloperoxidase and eosinophil peroxidase. This heme protein catalyzes H2O2‐driven radioiodinations, oxidations and formation of dityrosine. We propose that peroxidasin functions uniquely in extracellular matrix consolidation, phagocytosis and defense.
Effects of kynurenine on CD3+ and macrophages in wound healing

As prolongation of the inflammation phase in a healing process frequently leads to wound impairment, here we queried whether kynurenine (Kyn) could modulate this phase of wound healing. To address this, a protein microarray, quantitative polymerase chain reaction (qPCR), flow cytometry for immune cells and immune cell proliferation in the presence and absence of Kyn were conducted and compared. The result of a protein microarray revealed that the expression of 12 pro‐inflammatory cytokines and chemokines was modulated in Kyn‐treated mouse splenocytes as compared with those of control. These findings were then evaluated by conducting a qPCR for the gene expression of these factors and showed a significant reduction in the gene expression of majority of these cytokines and chemokines (interleukin [IL]‐2, IL‐17, C‐X‐C motif chemokine ligand [CXCL] 10, CXCL1, C‐C motif ligand [CCL] 12, CXCL9, CCL4, CXCL2, and CCL5) in response to Kyn treatment. To test the anti‐inflammatory effect of Kyn in an animal model, dorsal surface wounds were generated in a mouse model and wounds received daily topical application of either nothing (control), dermal cream (second control), or Kyn cream using uninjured skin tissue as another control. The wounded tissues were harvested on days 3, 6, and 10 postwounding. As anticipated, the results of fluorescence‐activated cell sorting analysis revealed that upon wounding, the number of total infiltrated CD3+ cells and macrophages (CD11b+) significantly increased on day 3, peaked on day 6, and reduced on day 10 post‐wounding. Interestingly, as compared with those of uninjured and dermal cream alone‐treated wounds, Kyn treatment significantly reduced the number of infiltrated CD3+ cells, but not CD11b+ cells, at different time intervals examined. These findings collectively suggest that Kyn, as a small molecule, can potentially be used to overcome the difficulties associated with persistency of inflammation in healing wounds.
Neighbour-distinguishing decompositions of graphs

Dans cette these nous explorons differentes decompositions de graphes. Le titre de la presente these est du au fait que la majorite de ces decompositions sont des decompositions voisin-distinguantes. En d'autres mots, nous pouvons en extraire des colorations propres des sommets. La question principale presentee dans cette these a ete introduite par Karonski, Łuczak et Thomason: Est il possible de ponderer les aretes d'un graphes avec les poids 1, 2 et 3, afin que tous les sommets voisins soient distingues par la somme des poids de leurs aretes incidentes ? Cette question deviendra plus tard la fameuse 1-2-3 Conjecture. Nous presentons differentes variantes de la 1-2-3 Conjecture, ainsi que leurs liens avec les decompositions localement irregulieres. Nous nous interessons tant a des problemes d'optimisation qu'a des problemes algorithmiques. Nous commencons par introduire une variante equitable des arete-ponderations voisin-somme-distinguantes, ou chaque poids doit etre utilise le meme nombre de fois (a l'unite pres). Ensuite nous presentons une variante injective ou chaque poids est utilise au plus une seule fois. Ce qui est un cas particulier de la variante equitable. De plus les ponderations injectives sont une variante locale des etiquetages anti-magiques. Ensuite nous modifions les conditions de distinction entre voisin en introduisant une variante 2-distinguante. les ponderations voisins-somme-2-distinguantes requierent que deux sommets voisins dans le graphe aient des sommes incidentes qui different d'au moins 2. Nous etudions le poids maximum minimal dans de telles ponderations pour certaines familles de graphes, ainsi que des problemes de complexite. Du aux liens entre les ponderations voisins-sommet-distinguantes et les decompositions localement irregulieres, nous nous sommes aussi interesse a ces dernieres, particulierement pour les graphes sub-cubiques, ainsi qu'a d'autres variantes des decompositions localement irregulieres. Finalement nous presentons un jeu de ponderations a deux joueurs, ainsi qu'une theorie de decompositions qui unifie les ponderations voisin-somme-distinguantes et les decompositions localement irregulieres.
[Clinical observasion of acupuncture in patients with depression and its impact on serum 5-HT].

OBJECTIVE To observe the clinical effect of acupuncture for depression and to discuss its impact on the content of 5-HT in patients with depression. METHODS Eighty patients with depression were randomly divided into an acupuncture group and a western medication group,40 cases in each one. Acupuncture was applied in the acupuncture group,Siman(KI 14),Shenshu(BL 23),Guanyuan(CV 4),Dazhui(GV 14),Yinlingquan(SP 9), Zusanli(ST 36),Taichong(LR 3),Yanglingquan(GB 34) and Jingming(BL 1) were selected, the intensive moxibustion was applied at G(uanyuan(CV 4). Fluoxetine was treated with oral administration in the western medication group. The treatments of six weeks were required in each group. The Hamilton depression rating scale (HAMD) was applied to evaluate efficacy and serum 5-HT was detected before and after treatment in the two groups. RESULTS After treatment,the scores of HAMD were decreased obviously in the two groups compared with those before treatment (scores in the acupuncture group: 24. 48 ± 0. 28 vs 8. 95 ± 2. 24; scores in the western medication group: 24. 14±0. 24 vs 10. 29±1. 30),and the differences were statistically significant (both P<0. 05). Between the two groups,the scores of HAMD in the acupuncture group at the end of the lst,2nd,4th,6th weeks were superior to those in the western medication group (all P<0. 05). The content of serum 5-HT after treatment was increased markedly compared with that before treatment [the content in the acupuncture group: (26. 21 2. 36)pg/mL vs (52. 07 ± 0. 56)pg/mL, the content in the western medication group:(26. 26±2. 31)pg/mL vs (51. 70±0. 52)pg/ mL, both P<0. 05]. But there was no statistic significance between the two groups(P >0.05). CONCLUSION The efficacy of acupuncture for depression is superior to that of western medication with fluoxetine.
Aproximación a la justificación de la atención selectiva a través de la percusión corporal - Método BAPNE

A traves de este articulo vamos a tratar de fundamentar la posible justificacion del metodo BAPNE en la estimulacion de la atencion selectiva segun el modelo propuesto por Sohlberg y Mateer. Para ello se han utilizado varias actividades del metodo, en el que es necesario un alto grado de atencion para seleccionar y mantener en presencia de estimulos distractores, distintas tareas.
English Proficiency and Labour Supply of Immigrants in Australia

This article explores the impact of English proficiency on labour supply of recent immigrants in Australia. Previous research has shown that English ability is important for participation and employment, however little research has been done with respect to hours worked. This study uses the second cohort of the Longitudinal Survey of Immigrants to Australia (LSIA) data to estimate a random effects Tobit model—the correct approach when the dependent variable is bounded by zero. To obtain consistent estimates of censored regression models with random effects, we employ the Chamberlain style Tobit random effects estimator. The second cohort arrived in 1999-2000 and includes two waves of LSIA data, with the waves being interviewed six and 18 months after arrival. The results suggest that English ability is positively related to the hours worked by immigrants. Therefore, if immigrants are proficient in English, they are more likely to be successful in the destination labour market.
CD31 Regulates Direction and Rate of Neutrophil Migration over and under Endothelial Cells

Mechanisms guiding migration of neutrophils through endothelium are poorly understood. We showed previously that CD31-CD31 binding acted as an ‘accelerator’ for neutrophils migrating on platelets, while neutrophil αvβ3-integrin acted as a sensor to align migration with the direction of imposed flow. Here, we perfused neutrophils over human umbilical vein endothelial cells (HUVEC) treated with tumour necrosis factor-α, and characterised the kinetics of migration over, through and underneath the HUVEC. Before penetrating the monolayer, activated neutrophils migrated relatively slowly over the surface (∼6 µm/min), preferentially in the direction of flow. Once transmigrated, neutrophils moved more rapidly (∼14 µm/min) without preferred direction. Treatment of HUVEC and/or neutrophils with function-blocking antibodies against CD31 reduced directionality but not velocity of migration on top of HUVEC, and reduced velocity of migration underneath the monolayer. If neutrophils were pre-activated with formyl peptide, they did not migrate through the HUVEC, but migrated with increased velocity and directionality on top. Under these circumstances, both velocity and directionality were reduced by blocking CD31. αvβ3-integrin did not regulate migration under any conditions. We conclude that CD31-CD31 bonds act as robust sensors which can guide neutrophil migration, and also modify its velocity. Thus mechanical and adhesive signals can regulate neutrophil migration driven by locally-acting chemotactic agents.
Estimation of the Tryptophan Requirement of Chickens for Maximum Body Weight Gain and Feed Efficiency

SUMMARY Tryptopahn is an essential amino acid for chickens and has many metabolic roles. The world’s literature on the tryptophan requirement of starting chickens (0 to 3 weeks) was reviewed. Data were pooled from 29 different experiments into two groups: broilers and other genotypes. The nonlinear mathematical models [ascending line with plateau (ALP) and ascending quadratic line with plateau (AQP)] were fitted to estimate the tryptophan requirement from these data sets. The tryptophan requirements estimated for body weight gain (BWG) and feed efficiency (FE) of broilers using the ALP model were 0.20 ± 0.002% (BWG) and 0.25 ± 0.2% (FE). Using the AQP model, the requirements were 0.24 ± 0.004% (BWG) and 0.29 ± 0.03% (FE). The tryptophan requirements for the other stocks using the ALP model were 0.24 ± 0.02% (BWG) and 0.20 ± 0.02% (FE). Using the AQP model, the requirements were 0.32 ± 0.06% (BWG) and 0.28 ± 0.05% (FE). Based on this analysis, it was concluded that the current National Research Council (NRC) [1] estimate of the tryptophan requirement (0.20% of the diet) is probably low.
Focal Adenitis in Salivary and Lacrimal Glands

The mechanism of production of Sjogren's syndrome (of which an oral manifestation is commonly dryness of the mouth) has been tentatively linked with that of rheumatoid arthritis by Morgan (1954) and with that of a 'broader spectrum of auto-immune disease' by Bunim (1961). The present work was designed to investigate the hypothesis that foci of lymphatic infiltration seen in salivary and lacrimal glands might be more common than was realized, that they might represent a focal form of Sj6gren's syndrome and that they might have the same associations with systemic disease entities as Sjogren's syndrome. The problem was approached in two ways: (1) by examination of post-mortem material and (2) by experimental attempts to reproduce the lesions in two species of laboratory animal. In regard to terminology: the view of Bunim (1961) that the term Sjogren's syndrome should be used in a comprehensive sense and should include 'Mikulicz's disease' is accepted. Focal adenitis of salivary and lacrimal glands will be defined as chronic inflammation of these glands apparently unconnected, at least until late in the process, with suppuration or duct obstruction and manifested by the presence of microscopical foci of lymphocytes, histiocytes and a few plasma cells, usually in association with small veins. Gland lobules, whether containing otherwise acceptable foci or not, in which polymorphonuclear leucocytes were found in any site outside blood vessels or in which there was any duct dilatation were specifically excluded from consideration.
Immunoglobulin G and immunoglobulin M enzyme-linked immunosorbent assays and defined toxoplasmosis serological patterns

Enzyme-linked immunosorbent assay (ELISA) for toxoplasmosis was evaluated in serum samples presenting defined toxoplasmosis serological patterns, as determined by results in immunoglobulin (Ig)G and IgM immunofluorescence (IgG-IF, IgM-IF), hemagglutination, and complement fixation tests. ELISA was carried out with alkaline phosphatase-labeled anti-IgG and anti-IgM antibodies. Serum titer was expressed as the serum dilution end point determined by mere observation of color development in the test. A straight agreement was found between IgG-ELISA and IgG-IF titers, both in group A sera of ancient infections (patterns II and III) and group B sera of recent infections (pattern I). A similar agreement was found between IgG-ELISA and hemagglutination titers in group A sera, for which coincident IgG-IF and hemagglutination titers are also frequent. However, in group B sera, in spite of the same toxoplasma extract being used to sensitize both plastic surfaces and erythrocytes, IgG-ELISA titers were much higher than hemagglutination titers, in a way similar to that observed for IgG-IF titers. IgM-ELISA was positive in every group B serum, with higher titers than corresponding IgM-IF titers. Occasional low-titered positive IgM-ELISA results were seen for group A sera, sometimes due to IgM-antiglobulin antibodies. An easy test to perform, ELISA seems to be an adequate substitute for toxoplasmosis IF tests for routine purposes.
Bacteriophages induced from weakly beta‐haemolytic human intestinal spirochaetes by mitomycin C

A comparative electron microscopic analysis of weakly beta‐haemolytic spirochaetes related to human and animal intestinal spirochaetosis was done in order to search for the presence of inducible bacteriophages associated with these spirochaetes. Bacteriophages were detected at the electron microscope after experimental induction with mitomycin C in 4 strains of weakly beta‐haemolytic spirochaetes related to human intestinal spirochaetosis, in Serpulina pilosicoli strain P43/6/78, the causative agent of swine intestinal spirochaetosis, in a spirochaetal strain related to avian intestinal spirochaetosis, and in Serpulina hyodysenteriae, strain P18A, the causative agent of swine dysentery, which was comparatively analysed as control. All phage‐particles observed in both human and animal intestinal spirochaetes were morphologically similar with an isometric head of 45 nm diameter and a tail 63–70 nm long and 7–12 nm width. The presence of morphologically similar phages in all the haemolytic intestinal spirochaetes of human and animal origin analysed in this study opens some important questions, about the genetic relationship of phages present in pathogenic intestinal spiro‐chaetes, their host range, and the possibility of natural gene transfer among pathogenic haemolytic intestinal spirochaetes of human and animal origin.
An attenuated influenza virus vaccine: Reactogenicity, transmissibility, immunogenicity, and protective efficacy.

An attenuated influenza virus vaccine (not sensitive to equine serum) was administered intranasally by nose drops and was effective in stimulating significant rises in both serum and nasal antibody and in protecting against subsequent challenge with virulent influenza virus. Vaccine virus was not transmitted from immunized volunteers to a control group. Clinical symptoms after immunization were very mild but were significantly more frequent in vaccinees than in members of the control group.
A systematic review of efficacy of machine-driven and manual subgingival debridement in the treatment of chronic periodontitis.

OBJECTIVES The purpose of this systematic review was to determine the efficacy of machine-driven compared with manual subgingival debridement in the treatment of periodontitis. BACKGROUND Mechanical debridement of the periodontal pocket plays a pivotal role in the treatment of periodontitis. METHODS A literature search for controlled clinical trials with at least 6 months' follow-up comparing machine-driven instruments with hand instruments for the treatment of chronic periodontitis was performed up to April 2001. Screening of titles and abstracts as well as data extraction was conducted independently by two reviewers (J.T. & T.F.F.). As primary outcome variable, the prevention of tooth loss was used; secondary outcome variables were the prevention of disease progression, the resolution of anatomical defects and the resolution of gingival inflammation. Efficiency was assessed by mean time needed to treat one tooth. RESULTS From a total of 419 abstracts, 27 articles were included for the review. The weighted kappa score for agreement between the two reviewers was 0.77, 95% CI: 0.65-0.89, indicating substantial agreement. No study reported on the selected primary outcome variables. Using clinical attachment gain, probing pocket depth reduction or bleeding on probing reduction as outcome variables, there appeared to be no differences between ultrasonic/sonic and manual debridement. No major differences in the frequency or severity of adverse effects were found. However no meta-analysis could be performed on any of the previously mentioned parameters. Ultrasonic/sonic debridement was found to take significantly less time, i.e. 36.6%, than debridement using hand instruments (P = 0.0002, 95% CI of the standardized effect estimate: 0.39-1.37, heterogeneity P = 0.77). CONCLUSIONS With respect to clinical outcome measures, the available data do not indicate a difference between ultrasonic/sonic and manual debridement in the treatment of chronic periodontitis for single-rooted teeth; however, the evidence for this is not very strong. In addition, ultrasonic/sonic subgingival debridement requires less time than hand instrumentation. Further research is needed to assess the efficacy of machine-driven debridement on multirooted teeth and clinical outcome variables having tangible benefit to the patients should be used.