Miracle-dz/UniFAR-FTUs huggingface dataset:
Determination of Ovarian Reserve with Anti-Mullerian Hormone in Patients with Polycystic Ovary Syndrome and Premenopausal Women

Objective: We aimed to evaluate the capacity of measuring serum anti-mullerian hormone (AMH) levels in determining ovarian reserve in patients with polycystic ovary syndrome (PCOS) with increased ovarian reserve and in premenopausal women with decreased ovarian reserve. Methods: Seventy-five patients who presented to our clinic between May-September 2011 were included. The patients comprised 25 (20-35 years) with PCOS, 25 (20-35 years) with normo-ovulatory cycle, and 25 (40-45 years) in the premenopausal period. Blood specimen was taken from patients during the early follicular period (on the third day of spontaneous menstrual cycles) and FSH, LH, estradiol (E2), and AMH levels were analyzed. In addition, body mass indexes (BMI) of the patients were calculated by measuring their heights and weights. Results: We determined statistically significant differences between groups regarding AMH values (p<0.001). We determined the mean AMH value of the PCOS group to be 58.45±33.68 pmol/L, 19.92±21.35 pmol/L for the control group, and 2.47±5.31 pmol/L for the premenopausal group. We found a statistically significant negative relationship between AMH and BMI levels in all cases without discrimination (r:-0,277; p=0.016), and we found a statistically significant positive relationship between AMH and AFC (antral follicle count) (r: 0.908; p=<0.001). Conclusion: AMH is a highly specific biomarker in determining ovarian reserve. High levels of AMH values in patients with PCOS and low levels in premenopausal women demonstrate that AMH is a safe biomarker in determining ovarian reserve. (JAREM 2014; 2: 62-8)
Linking aberrant chromatin features in chronic lymphocytic leukemia to transcription factor networks

In chronic lymphocytic leukemia (CLL), a diverse set of genetic mutations is embedded in a deregulated epigenetic landscape that drives cancerogenesis. To elucidate the role of aberrant chromatin features, we mapped DNA methylation, seven histone modifications, nucleosome positions, chromatin accessibility, binding of EBF1 and CTCF, as well as the transcriptome of B cells from CLL patients and healthy donors. A globally increased histone deacetylase activity was detected and half of the genome comprised transcriptionally downregulated partially DNA methylated domains demarcated by CTCF. CLL samples displayed a H3K4me3 redistribution and nucleosome gain at promoters as well as changes of enhancer activity and enhancer linkage to target genes. A DNA binding motif analysis identified transcription factors that gained or lost binding in CLL at sites with aberrant chromatin features. These findings were integrated into a gene regulatory enhancer containing network enriched for B‐cell receptor signaling pathway components. Our study predicts novel molecular links to targets of CLL therapies and provides a valuable resource for further studies on the epigenetic contribution to the disease.
A comparative analysis of suicide attempts in left-behind children and non-left-behind children in rural China

To estimate the prevalence of suicide attempts and explore the shared and unique factors influencing suicide risk in left-behind children (LBC) and non-left-behind children (NLBC) in rural China, this study collected data using a multi-stage cluster random sampling method from 13,952 children including 6,034 LBC and 7,918 NLBC. Sociodemographic characteristics, suicide attempts, neglect and physical abuse, negative life events, and loneliness were measured by self-reported questionnaires. Data were analyzed using logistic regression models. Gender and mother's education level were unique influential factors for NLBC while family structure type was a unique influential factor for LBC. The study provides two novel findings regarding NLBC specifically: 1. Children with optimal family socioeconomic status are more likely to report suicide attempts (odds ratio OR = 1)than are those in the general children population, OR 0.52 (95% CI: 0.39–0.70), and 2. Children with higher mother’s education level are subject to higher suicide rates in high school, OR 1.67 (95% CI: 1.13–2.46), and post-secondary education, OR 2.14 (95% CI: 1.37–3.37). The unique characteristics of LBC and NLBC in China suggest that investigating risk factors and determining the factors that might be targeted in intervention programs are urgently needed currently.
Access to hypertension care and services in primary health-care settings in Vietnam: a systematic narrative review of existing literature

ABSTRACT Background: Health care in Vietnam is challenged by a high burden of hypertension (HTN). Since 2000, several interventions were implemented to manage HTN; it is not clear what is the status of patient access to HTN care. Objective: This article aims to perform a systematic narrative review of the available evidence on access to HTN care and services in primary health-care settings in Vietnam. Methods: Search engines were used to identify relevant records of scientific and grey literature. Data from selected articles were analysed using standardised spreadsheets and MaxQDA and following a framework synthesis methodology. Results: There has been increasing interest in research and policy concerning the burden of HTN in Vietnam, covering many aspects of access to treatment at the primary health-care level. Vietnam’s National HTN Programme is managed as a vertical programme and its services integrated into the network of primary health-care facilities across the public sector in selected provinces. The Programme financed population-wide screening campaigns for the early detection of HTN among people above 40 years of age. There was no information on the acceptability of HTN health services, especially regarding the interaction between patients and health professionals. In general, articles reported good availability of medication, but problems in accessing them included: fragmentation and lack of consistency in prescribing medication between different levels and short timespans for dispensing medication at primary health-care facilities. There was limited information related to the cost and economic impact of HTN treatment. Treatment adherence among hypertensive patients based on four studies did not exceed 70%. Conclusions: Although the Vietnamese health-care system has taken steps to accommodate some of the needs of HTN patients, it is crucial to scale-up interventions that allow for regular, systematic, and integrated care, especially at the lowest levels of care.
Acid Sensitive Ion channels are expressed in human induced pluripotent stem cell-derived cardiomyocytes.

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are potential sources for cardiac regeneration and drug development. hiPSC-CMs express all the cardiac ion channels and the unique cardiac Ca2+-signaling phenotype. Here we tested for expression of acid sensing ion channels (ASIC) in spontaneously beating cardiomyocytes derived from three different hiPSC lines (IMR-90, iPSC-K3 and Ukki011-A). Rapid application of solutions buffered at pH 6.7, 6.0, or 5.0 triggered rapidly activating and slowly inactivating voltage-independent inward current that reversed at voltages positive to ENa, was suppressed by 5µM amiloride and withdrawal of [Na+]o , like neuronal ASIC currents. ASIC currents were expressed at much lower percentages and densities in undifferentiated hiPSC, and in dermal fibroblasts. ASIC1 mRNA and protein were measured in first 60 days but not 100-days post-differentiation hiPSC cultures. Hyper-acidification (pH5-6) also triggered large Ca2+ transients in intact hiPSC-CMs that were neither ruthenium red nor amiloride-sensitive, but were absent in whole cell-clamped hiPSC-CMs. Neither ASIC1 currents nor its protein were detected in rat adult cardiomyocytes, but hyper-acidification did activate smaller and slowly activating currents with drug sensitivity similar to TRPV channels. Considering ASIC expression in developing but not adult myocardium, a role in heart development is likely.
Extracting shape reflectance of Lambertian, specular, and hybrid surfaces

Abstract : All existing shape extraction techniques that are based on photometric measurements rely on assumed surface reflection properties. A method is proposed for determining the shape of surfaces whose reflectance properties may vary from Lambertian to specular without prior knowledge of the relative strengths of the Lambertian and specular components of reflection. The object surface is illuminated using extended light sources and is viewed from a single direction. Surface illumination using extended sources makes it possible to ensure the detection of both Lambertian and specular reflections. Multiple source directions are used to obtain an image sequence of the object. An extraction algorithm uses the set of image intensity values measured at each surface point to compute orientation as well as the relative strengths of the Lambertian and specular reflection components. The proposed methods has been named photometric sampling as it uses samples of a photometric function that relates image intensity to surface orientation, reflectance, and light source characteristics that describes the reflectance model and orientation of a surface point. Experiments were conducted on Lambertian surfaces, specular surfaces, and hybrid surfaces whose reflectance model is composed of both Lambertian and specular components. The results show high accuracy in measured orientations and estimated reflectance parameters. Keywords: Extraction techniques; Photometric measurements; Reflectance properties; Specular reflectance; Photometric sampling.
Comparative fishing trials for Charybdis japonica using collapsible box-shaped and dome-shaped pots

Comparative fishing trials were undertaken with two commercial collapsible pots for the crab Charybdis japonica. One pot was box-shaped with two slit entrances at the ends, while the other was dome-shaped with two open funnel entrances. Pots were placed at the bottom of a pond that is connected to Kagoshima Bay. Fishing trials were carried out using a 1-day soaking time; the bait used was mackerel. The box-shaped pot had higher catches of crabs and non-target organisms, ***DIRECT SUPPORT *** A02OT006 00004 but the number of large crabs (carapace width \s>8 cm) was almost half as many as that caught with the dome-shaped pot. The catch of non-target species was also greatly reduced in the dome-shaped pot, and fish caught were mostly enmeshed in the netting. The reduction in catches of small crabs and non-target organisms was due to the nature of the dome-shaped pot’s larger mesh size and open entrances, which allowed for escape. The dome-shaped pot seemed more efficient for harvesting commercial size crabs, while the box-shaped pot seemed better for removing all crab sizes.
Complementary congruent and opposite neurons achieve concurrent multisensory integration and segregation

Our brain perceives the world by exploiting multisensory cues to extract information about various aspects of external stimuli. The sensory cues from the same stimulus should be integrated to improve perception, and otherwise segregated to distinguish different stimuli. In reality, however, the brain faces the challenge of recognizing stimuli without knowing in advance the sources of sensory cues. To address this challenge, we propose that the brain conducts integration and segregation concurrently with complementary neurons. Studying the inference of heading-direction via visual and vestibular cues, we develop a network model with two reciprocally connected modules modeling interacting visual-vestibular areas. In each module, there are two groups of neurons whose tunings under each sensory cue are either congruent or opposite. We show that congruent neurons implement integration, while opposite neurons compute cue disparity information for segregation, and the interplay between two groups of neurons achieves efficient multisensory information processing.
Developing a prediction rule to assess hepatic malignancy in patients with cirrhosis.

OBJECTIVE The objectives of our study were to identify independent clinical, demographic, and MR imaging correlates of malignancy in patients with cirrhosis and to develop a predictive model based on identified correlates of malignancy. MATERIALS AND METHODS Sixty examinations of 58 patients with biopsy proof of lesions suggestive of hepatocellular carcinoma on MR imaging were retrospectively reviewed. The signal intensity of the lesion on T2-weighted imaging and dynamic gadolinium-enhanced imaging, the size of the lesion, and the number of suspicious lesions were recorded; in addition, patient age and sex, alpha-fetoprotein level, and hepatitis C viral genotype were noted. The association between malignancy and each predictor variable was evaluated using the chi-square test or the two-group t test. The final logistic regression model included the variables that were shown to have a significant association with malignancy and the clinically relevant predictors. We used the adjusted odds ratios to measure the strength of each association. The discriminant ability of the model for detecting hepatic malignancy was assessed using receiver operating characteristic curve analysis. RESULTS The prevalence of hepatic malignancy in our study population was 64%. The area under the receiver operating characteristic curve for the logistic regression model was 0.82. Venous washout (odds ratio = 9.2), alpha-fetoprotein level (odds ratio = 3.2), and number of lesions (odds ratio = 1.5) were significant predictors for malignancy (p < 0.05). When arterial enhancement and venous washout were either both present or both absent, alpha-fetoprotein level contributed little to the prediction of malignancy. CONCLUSION The MR characteristics of hepatic lesions during the dynamic venous phase in conjunction with the serum alpha-fetoprotein level and number of lesions are predictors of hepatic malignancy. The use of these predictors can facilitate explicit estimation of malignancy in individuals with underlying cirrhosis, potentially improving clinical decision-making.
Physical exercise and human adipose‐derived mesenchymal stem cells ameliorate motor disturbances in a male rat model of Parkinson's disease

Parkinson's disease (PD) is a disabling and highly costly neurodegenerative condition with worldwide prevalence. Despite advances in treatments that slow progression and minimize locomotor impairments, its clinical management is still a challenge. Previous preclinical studies, using mesenchymal stem cell (MSC) transplantation and isolated physical exercise (EX), reported beneficial results for treatment of PD. Therefore, this experimental randomized study aimed to elucidate the therapeutic potential of combined therapy using adipose‐derived human MSCs (ADSCs) grafted into the striatum in conjunction with aerobic treadmill training, specifically in terms of locomotor performance in a unilateral PD rat model induced by 6‐hydroxydopamine (6‐OHDA). Forty‐one male Wistar rats were categorized into five groups in accordance with the type of treatment to which they were subjected (Sham, 6‐OHDA − injury, 6‐OHDA + exercise, 6‐OHDA + cells, and 6‐OHDA + combined). Subsequently, dopaminergic depletion was assessed by the methylphenidate challenge and the specified therapeutic intervention was conducted in each group. The foot fault task was performed at the end of the experiment to serve as an assessment of motor skills. The results showed that despite disturbances in motor balance and coordination, locomotor dysfunction was ameliorated in all treatment categories in comparison to the injury group (sign test, p < 0.001, effect size: 0.71). The exercise alone and combined groups were the categories that exhibited the best recovery in terms of movement performance (p < 0.001). Overall, this study confirms that exercise is a powerful option to improve motor function and a promising adjuvant intervention for stem cell transplantation in the treatment of PD motor symptoms.
The evolution of online ideological communities

Online communities are virtual spaces for users to share interests, support others, and to exchange knowledge and information. Understanding user behavior is valuable to organizations and has applications from marketing to security, for instance, identifying leaders within a community or predicting future behavior. In the present research, we seek to understand the various roles that users adopt in online communities–for instance, who leads the conversation? Who are the supporters? We examine user role changes over time and the pathways that users follow. This allows us to explore the differences between users who progress to leadership positions and users who fail to develop influence. We also reflect on how user role proportions impact the overall health of the community. Here, we examine two online ideological communities, RevLeft and Islamic Awakening (N = 1631; N = 849), and provide a novel approach to identify various types of users. Finally, we study user role trajectories over time and identify community “leaders” from meta-data alone. Study One examined both communities using K-MEANS cluster analysis of behavioral meta-data, which revealed seven user roles. We then mapped these roles against Preece and Schneiderman’s (2009) Reader-to-Leader Framework (RtLF). Both communities aligned with the RtLF, where most users were “contributors”, many were “collaborators”, and few were “leaders”. Study Two looked at one community over a two-year period and found that, despite a high churn rate of users, roles were stable over time. We built a model of user role transitions over the two years. This can be used to predict user role changes in the future, which will have implications for community managers and security focused contexts (e.g., analyzing behavioral meta-data from forums and websites known to be associated with illicit activity).
Production , Manufacturing and Logistics Inventory model of deteriorated items with a constraint : A geometric programming approach

An inventory model for deteriorating items is built-up with limited storage space. Here, demand rate for the items is finite, items deteriorate at constant rates and are replenished instantaneously. Following EOQ model, the problem is formulated with and without truncation on the deterioration term and ultimately is converted to the minimization of a signomial expression with a posynomial constraint. It is solved by modified geometric programming (MGP) method and non-linear programming (NLP) method. The problem is supported by numerical examples. The results from two versions of the model (with and without truncation) and two methods (i.e. MGP and NLP) are compared. 2005 Elsevier B.V. All rights reserved.
Diagnostic Performance of MALDI-TOF MS Compared to Conventional Microbiological Cultures in Patients with Suspected Endophthalmitis

ABSTRACT Purpose: To evaluate the performance and speed of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) when identifying the pathogenic microorganism of endophthalmitis compared to conventional microbiological culturing. Methods: Forty-four patients with suspected endophthalmitis who had undergone vitrectomy were enrolled. Vitreous specimen was analyzed using either conventional culturing or MALDI-TOF MS. Results: The identification rates of the conventional microbiological culture and MALDI-TOF MS were 45.5% (20/44) and 65.9% (29/44), respectively (Kappa value 0.787, P < 0.000). The mean detection times by the standard culturing method and MALDI-TOF MS were 5.39 ± 0.56d and 3.17 ± 0.40d (P < 0.001). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of MALDI-TOF MS were 70.59%, 54.17%, 80.00%, and 86.67%, respectively. Polymicrobial endophthalmitis was identified in 6.82% of the patients (3/44) using conventional microbiological culturing. However, MALDI-TOF MS failed to identify any polymicrobial infection. Conclusions: With a higher sensitivity, acceptable specificity and a shorter detection time, MALDI-TOF MS was an efficient technique for the rapid identification of a pathogenic microorganism in endophthalmitis.
A novel synthetic inhibitor of factor Xa decreases early reocclusion and improves 24-h patency after coronary fibrinolysis in dogs.

Inhibition of factor Xa (FXa) attenuates thrombus progression. This study was designed to determine whether a novel, synthetic inhibitor of FXa (ZK-807834, molecular mass 527 Da, K(i) = 0.11 nM) administered during and briefly after pharmacologic coronary fibrinolysis increases 24-h patency. Either ZK-807834 (< or = 1.6 mg/kg, n = 10; 6.5 mg/kg, n = 8; or 13 mg/kg, n = 7); a peptide inhibitor of FXa, recombinant tick anticoagulant peptide (rTAP, 13.6 mg/kg, n = 7); heparin (150 U/kg bolus and 50 U/kg/h infusion) and aspirin (5 mg/kg) (n = 7); or saline as a control (n = 13) were administered i.v. over 135 min in conscious dogs after thrombotic occlusion induced by electrical injury to a coronary artery. Fibrinolysis was induced with recombinant human tissue-type plasminogen activator (1.0 mg/kg i.v. over 1 h), and patency was monitored continuously for 24 h with an implanted Doppler probe. Reocclusion occurred in all control and heparin/aspirin-treated dogs within 1 h after fibrinolysis. High dose ZK-807834 prevented reocclusion in five of six dogs and delayed reocclusion in the other dog (186 min after recanalization, p = 0.0005 versus heparin/aspirin). Reocclusion was delayed (406 +/- 329 min), but still occurred in three of six rTAP-treated dogs (p = 0.003 versus heparin/aspirin). Patency after 24 h was 100% in ZK-807834-treated and rTAP-treated dogs compared with 67% in control and 83% in heparin/aspirin-treated dogs. PT was increased 3.7-fold, activated partial thromboplastin time 4.9-fold, and bleeding time 2.5-fold by high dose ZK-807834 compared with 1.2-fold, 11.5-fold, and 2.3-fold, respectively, for heparin/aspirin. Inhibition of FXa with ZK-807834 decreases reocclusion and improves patency of recanalized arteries without increasing bleeding compared with heparin/aspirin.