Miracle-dz/UniFAR-FTUs huggingface dataset:
Bacterial Translocation and Risk of Liver Cancer in a Finnish Cohort

Background: Elevated systemic exposure to gut-derived bacterial products has been associated with hepatic inflammation and chronic liver diseases, potentially increasing the risk of liver cancer. However, only one prior study prospectively examined exposure to bacterial products in the circulation and risk of liver cancer, with a relatively limited coverage of biomarkers. Methods: We conducted a nested case–control study (224 liver cancer cases and 224 matched controls) in a large cohort of Finnish male smokers followed from baseline (1985–1988) to 2014. The associations between a panel of biomarkers for bacterial translocation and the risk of liver cancer were assessed using multivariable-adjusted conditional logistic regression. The biomarkers included immunoglobulin (Ig) A, IgG, and IgM against lipopolysaccharide (LPS) and flagellin, soluble CD14 (an LPS coreceptor), and the LPS-binding protein. Results: Anti-flagellin IgA [odds ratios (OR), 2.79; 95% confidence intervals (CI), 1.34–5.78; Ptrend = 0.01] and anti-LPS IgA (2.44; 95% CI, 1.33–4.48; Ptrend < 0.01) were significantly associated with risk of liver cancer. When restricting the analysis to histologically classified hepatocellular carcinoma, the ORs were 4.18 (95% CI, 1.60–10.92; Ptrend < 0.01) and 2.48 (95% CI, 1.16–5.29; Ptrend < 0.01), respectively. The results were not substantially changed after excluding cases diagnosed within the first 5 years of follow-up and those with hepatitis C virus infection. Conclusions: Antibodies to flagellin and LPS were associated with increased risk of liver cancer. Impact: Gut-derived bacterial translocation into the circulation may play a role in the development of primary liver cancer. Our findings could contribute to the understanding of primary liver cancer etiology and further prevention efforts.
Plasmodium life cycle stage classification based quantification of malaria parasitaemia in thin blood smears

Visual inspection for the quantification of malaria parasitaemiain (MP) and classification of life cycle stage are hard and time taking. Even though, automated techniques for the quantification of MP and their classification are reported in the literature. However, either reported techniques are imperfect or cannot deal with special issues such as anemia and hemoglobinopathies due to clumps of red blood cells (RBCs). The focus of the current work is to examine the thin blood smear microscopic images stained with Giemsa by digital image processing techniques, grading MP on independent factors (RBCs morphology) and classification of its life cycle stage. For the classification of the life cycle of malaria parasite the k‐nearest neighbor, Naïve Bayes and multi‐class support vector machine are employed for classification based on histograms of oriented gradients and local binary pattern features. The proposed methodology is based on inductive technique, segment malaria parasites through the adaptive machine learning techniques. The quantification accuracy of RBCs is enhanced; RBCs clumps are split by analysis of concavity regions for focal points. Further, classification of infected and non‐infected RBCs has been made to grade MP precisely. The training and testing of the proposed approach on benchmark dataset with respect to ground truth data, yield 96.75% MP sensitivity and 94.59% specificity. Additionally, the proposed approach addresses the process with independent factors (RBCs morphology). Finally, it is an economical solution for MP grading in immense testing.
Re-envisioning reading comprehension for english language learners a critical point of view

When teaching a second language, it is important to bear in mind that the four skills: speaking, writing, listening, and reading must be facilitated and developed with the same relevance. However, some learners might tell their teacher they can understand everything he says, but they are unable to produce; or that they can understand a simple text, but when they face comprehension questions that require a critical level of understanding, they do not perform as expected. It is well known that productive skills depend on receptive skills (listening and reading) due to these serve as models for written and oral production; even though one of the most common problems teachers face with students and one of the most complex processes during teaching is oral and written comprehension, we could say that learners are more exposed to listening than to reading practice. It is to say, learners listen to their English teacher in the classroom and they listen to music in English, but second language learners do not have reading habits not even in their mother tongue. Reading is a process that involves attention, creativity, and reading strategies to develop effective comprehension from a text; throughout many years, authors support different theories in which they state the importance of involving students in real contexts. Our attempt in this paper is to point out the idea that reading is not an activity of translation, as some learners might think, rather a task that entails taking the meaning of words within a meaningful context for learners. Besides, we strongly believe that learners will read better in their second language if they learn about text’s characteristics and if they think about their own learning process, using a set of strategies to grasp meaning from texts. The main article Re-envisioning Reading Comprehension for English Language Learners, mentions some strategies that, from our own teaching experience, did not work as expected, and others that can be taken into further consideration in our teaching practice.
Mental health symptoms and the reintegration difficulty of military couples following deployment: A longitudinal application of the relational turbulence model.

OBJECTIVE Understanding the factors that predict the reintegration difficulty of military couples during the postdeployment transition has important implications for theory, research, and practice. Building on the logic of the relational turbulence model, this paper evaluates the relationship processes of reunion uncertainty and reintegration interference from a partner as mediators of the connection between people's mental health symptoms and their difficulty with reintegration after deployment. METHOD Dyadic longitudinal data were collected from 555 US military couples once per month for 8 consecutive months. RESULTS Findings mapped the trajectory of reintegration difficulty and suggested reunion uncertainty and reintegration interference from a partner as mediators of the link between people's depressive and posttraumatic stress symptoms and the magnitude of their reintegration difficulty. CONCLUSION These results highlight relationship processes as a key domain of intervention to preserve the well-being of military couples during the postdeployment transition.
Putative virulence factors of extra‐intestinal Escherichia coli isolated from bovine mastitis with different clinical scores

We investigated the genes kpsMTII, iucD, sfaDE, afaBC, papA and papC, (proposed to be involved in extra‐intestinal pathogenic Escherichia coli—ExPEC), phylogroup classification and the in vitro swimming and swarming motility in 50 E. coli isolated from bovine mastitis with different clinical severity scores (mild, moderate and severe). The aforementioned genes were detected in 12 (n = 12/50; 24·0%) isolates. kpsMTII and iucD were the most frequent genes identified in six (n = 6/50; 12·0%) and four (n = 4/50; 8·0%) of the isolates, respectively. In only one (n = 1/50; 2·0%) isolate, more than one gene was simultaneously identified: iucD and kpsMTll were detected whereas sfaDE and afaBC were not detected. Mild, moderate and severe clinical signs were observed in 40·0% (n = 20/50), 28·0% (n = 14/50) and 32·0% (n = 16/50) of the cases. Commensal phylogroups B1 (n = 19/50; 38·0%) and A (n = 19/50; 38·0%) were prevalent; whereas pathogenic phylogroups B2 and D were observed in only 10·0% (n = 5/50). Swarming and swimming motilities were observed in 90·0% (n = 45/50) and 68·0% (n = 34/50) of the isolates, respectively; and there was a significant association (P = 0·0036) between swarming motility and severe clinical cases (score 3). To the best of our knowledge, this is the first study where clinical severity of bovine mastitis cases and the genes proposed to classify ExPEC were assessed in relation to swarming and swimming motility.
Bronchoscopic lung cryobiopsy: An Indian Association for Bronchology position statement

Background: Bronchoscopic lung cryobiopsy (BLC) is a novel technique for obtaining lung tissue for the diagnosis of diffuse parenchymal lung diseases. The procedure is performed using several different variations of technique, resulting in an inconsistent diagnostic yield and a variable risk of complications. There is an unmet need for standardization of the technical aspects of BLC. Methodology: This is a position statement framed by a group comprising experts from the fields of pulmonary medicine, thoracic surgery, pathology, and radiology under the aegis of the Indian Association for Bronchology. Sixteen questions on various technical aspects of BLC were framed. A literature search was conducted using PubMed and EMBASE databases. The expert group discussed the available evidence relevant to each question through e-mail and a face-to-face meeting, and arrived at a consensus. Results: The experts agreed that patients should be carefully selected for BLC after weighing the risks and benefits of the procedure. Where appropriate, consideration should be given to perform alternate procedures such as conventional transbronchial biopsy or subject the patient directly to a surgical lung biopsy. The procedure is best performed after placement of an artificial airway under sedation/general anesthesia. Fluoroscopic guidance and occlusion balloon should be utilized for positioning the cryoprobe to reduce the risk of pneumothorax and bleeding, respectively. At least four tissue specimens (with at least two of adequate size, i.e., ≥5 mm) should be obtained during the procedure from different lobes or different segments of a lobe. The histopathological findings of BLC should be interpreted by an experienced pulmonary pathologist. The final diagnosis should be made after a multidisciplinary discussion. Finally, there is a need for structured training for performing BLC. Conclusion: This position statement is an attempt to provide practical recommendations for the performance of BLC in DPLDs.
Diabetes Self-Management and Glycemic Control: The Role of Autonomy Support From Informal Health Supporters

Objective: Effective diabetes self-management can prevent long-term health complications but is often complex and difficult to achieve. Health care professionals’ support for patients’ autonomy (autonomy support) in managing their diabetes contributes to better diabetes self-care and glycemic control. Most adults with diabetes also receive self-management support from informal health supporters. Yet, the role of autonomy support from these informal health supporters has not been explored. We examined patients’ perceived autonomy support from their main health supporter (family member or friend) in relation to their diabetes distress, self-efficacy, self-monitoring of blood glucose (SMBG), medication adherence, and hemoglobin A1c (HbA1c). Method: Three hundred twenty-six veterans with Type 2 diabetes at high risk for complications, who identified a main health supporter, were surveyed using self-report measures of social support, diabetes distress, self-efficacy, and self-care. HbA1c and prescription fill data were extracted from VA electronic records for the 12-months before and after the survey. Linear regression and linear mixed models examined the associations of autonomy support with diabetes distress, self-efficacy, SMBG, medication adherence, and glycemic control, controlling for participant-supporter relationship characteristics and patient-factors. Results: In adjusted models, greater autonomy support was associated with lower diabetes distress (B = −.323, SE = .098, p = .001), greater self-efficacy (B = .819, SE = .148, p < .001), more frequent SMBG (B = .297, SE = .082, p < .001), and better subsequent 12-month glycemic control (B = .257, SE = .085, p = .003) but not with medication adherence (B = .001, SE = .020, p = .994). Conclusions: Autonomy support from main health supporters is significantly associated better diabetes related attitudes, self-care, and glycemic control after adjusting for relationship and patient characteristics.
Drug Overdose Deaths Among Women Aged 30–64 Years — United States, 1999–2017

The drug epidemic in the United States continues to evolve. The drug overdose death rate has rapidly increased among women (1,2), although within this demographic group, the increase in overdose death risk is not uniform. From 1999 to 2010, the largest percentage changes in the rates of overall drug overdose deaths were among women in the age groups 45-54 years and 55-64 years (1); however, this finding does not take into account trends in specific drugs or consider changes in age group distributions in drug-specific overdose death rates. To target prevention strategies to address the epidemic among women in these age groups, CDC examined overdose death rates among women aged 30-64 years during 1999-2017, overall and by drug subcategories (antidepressants, benzodiazepines, cocaine, heroin, prescription opioids, and synthetic opioids, excluding methadone). Age distribution changes in drug-specific overdose death rates were calculated. Among women aged 30-64 years, the unadjusted drug overdose death rate increased 260%, from 6.7 deaths per 100,000 population (4,314 total drug overdose deaths) in 1999 to 24.3 (18,110) in 2017. The number and rate of deaths involving antidepressants, benzodiazepines, cocaine, heroin, and synthetic opioids each increased during this period. Prescription opioid-related deaths increased between 1999 and 2017 among women aged 30-64 years, with the largest increases among those aged 55-64 years. Interventions to address the rise in drug overdose deaths include implementing the CDC Guideline for Prescribing Opioids for Chronic Pain (3), reviewing records of controlled substance prescribing (e.g., prescription drug monitoring programs, health insurance programs), and developing capacity of drug use disorder treatments and linkage to care, especially for middle-aged women with drug use disorders.
Genetic Analysis of the Organization, Development, and Plasticity of Corneal Innervation in Mice

The cornea has the densest sensory innervation of the body, originating primarily from neurons in the trigeminal ganglion. The basic principles of cornea nerve patterning have been established many years ago using classic neuroanatomical methods, such as immunocytochemistry and electrophysiology. Our understanding of the morphology and distribution of the sensory nerves in the skin has considerably progressed over the past few years through the generation and analysis of a variety of genetically modified mouse lines. Surprisingly, these lines were not used to study corneal axons. Here, we have screened a collection of transgenic and knockin mice (of both sexes) to select lines allowing the visualization and genetic manipulation of corneal nerves. We identified multiple lines, including some in which different types of corneal axons can be simultaneously observed with fluorescent proteins expressed in a combinatorial manner. We also provide the first description of the morphology and arborization of single corneal axons and identify three main types of branching pattern. We applied this genetic strategy to the analysis of corneal nerve development and plasticity. We provide direct evidence for a progressive reduction of the density of corneal innervation during aging. We also show that the semaphorin receptor neuropilin-1 acts cell-autonomously to control the development of corneal axons and that early axon guidance defects have long-term consequences on corneal innervation. SIGNIFICANCE STATEMENT We have screened a collection of transgenic and knockin mice and identify lines allowing the visualization and genetic manipulation of corneal nerves. We provide the first description of the arborization pattern of single corneal axons. We also present applications of this genetic strategy to the analysis of corneal nerve development and remodeling during aging
Effect of Initial Intensive Insulin Therapy Followed by Sitagliptin on ? Cell Function in Patients with New Onset Type 2 Diabetes

Objective: It is established that early insulin therapy can improve both β cell function and glycaemic control in newly diagnosed type 2 diabetic patients. The dipeptidyl peptidase-4 (DPP-4) inhibitor, sitagliptin, can preserve or even increase the number of β cells in animal models of diabetes. Therefore, we aimed to determine whether treatment with sitagliptin after initial intensive insulin therapy would further reduce glycaemia and preserve β cell function in new-onset type 2 diabetes. Methods: 48 Chinese patients with newly diagnosed type 2 diabetes (fasting blood glucose concentration 13.42 ± 0.38 mmol/L; HbA1c:11.8 ± 0.2%) were recruited. All received insulin pump therapy for two weeks, followed by sitagliptin (100mg orally once daily) for three months. Arginine tests were performed at baseline, after two-weeks’ insulin pump therapy, and after 3 months’ sitagliptin therapy. Blood samples were collected at baseline, before and after the treatment with sitagliptin for measurement of blood glucose, plasma insulin and lipids profiles. β cell function was evaluated by HOMA-β and the insulin response to arginine. Results: Fasting blood glucose concentrations were substantially decreased after two weeks’ insulin therapy (P<0.01), and were further reduced after 3 months’ treatment with sitagliptin (P<0.01). HOMA-β and HOMA-IR were improved (P<0.01) after two weeks’ treatment with insulin, while HOMA-β was further improved after 3 months’ sitagliptin (P<0.01). However, the insulin response to arginine did not increase after two weeks’ insulin therapy, but did improve after sitagliptin (P<0.05). Conclusions: Intensive insulin therapy improved both glycaemic control and β cell function in newly diagnosed Chinese type 2 diabetes, and the improvements in β cell function was preserved after 3 months of sitagliptin.
The Autoimmune Susceptibility Gene C5orf30 Regulates Macrophage-Mediated Resolution of Inflammation

Genetic variants in C5orf30 have been associated with development of the autoimmune conditions primary biliary cirrhosis and rheumatoid arthritis. In rheumatoid arthritis, C5orf30 expression is cell-specific, with highest expression found in macrophages and synovial fibroblasts. C5orf30 is highly expressed in inflamed joints and is a negative regulator of tissue damage in a mouse model of inflammatory arthritis. Transcriptomic analysis from ultrasound-guided synovial biopsy of inflamed joints in a well characterized clinical cohort of newly diagnosed, disease-modifying antirheumatic drugs–naive rheumatoid arthritis patients was used to determine the clinical association of C5orf30 expression with disease activity. A combined molecular and computational biology approach was used to elucidate C5orf30 function in macrophages both in vitro and in vivo. Synovial expression of C5orf30 is inversely correlated with both clinical measures of rheumatoid arthritis disease activity and with synovial TNF mRNA expression. C5orf30 plays a role in regulating macrophage phenotype and is differentially turned over in inflammatory and anti-inflammatory macrophages. Inhibition of C5orf30 reduces wound healing/repair–associated functions of macrophages, reduces signaling required for resolution of inflammation, and decreases secretion of anti-inflammatory mediators. In an animal model of wound healing (zebrafish), C5orf30 inhibition increases the recruitment of macrophages to the wound site. Finally, we demonstrate that C5orf30 skews macrophage immunometabolism, demonstrating a mechanism for C5orf30-mediated immune regulation.
The impact of chronic non-specific cough on children and their families: A narrative literature review

The aim of this article is to critically appraise and synthesize research that examines the impact chronic non-specific cough has on children and their families and to highlight gaps within the research. Chronic non-specific cough refers to a persistent cough without a specific diagnosis. While studies have begun to examine the impact on children and their families, this research has not been synthesized and appraised. A narrative literature review was undertaken. A comprehensive and systematic search was undertaken, using CINAHL, MEDLINE, British Nursing Index, PsycINFO, Cochrane Wiley Library and ASSIA databases. Studies were critically appraised for quality using the Hawker et al.’s appraisal tool. A narrative review of the findings was undertaken. Nine quantitative studies were included in the review. The article suggests that chronic non-specific cough affects the quality of life of both families and children, affecting quality of sleep, impacting upon participation in activities, causing emotional distress and creating substantial demand on the health service. Furthermore, the research highlighted the worries experienced by parents in relation to the cause of their child’s cough. The review did not identify any qualitative research in this area and only one study collected data directly from children.
Human Exonuclease 1 (EXO1) Regulatory Functions in DNA Replication with Putative Roles in Cancer

Human exonuclease 1 (EXO1), a 5'→3' exonuclease, contributes to the regulation of the cell cycle checkpoints, replication fork maintenance, and post replicative DNA repair pathways. These processes are required for the resolution of stalled or blocked DNA replication that can lead to replication stress and potential collapse of the replication fork. Failure to restart the DNA replication process can result in double-strand breaks, cell-cycle arrest, cell death, or cellular transformation. In this review, we summarize the involvement of EXO1 in the replication, DNA repair pathways, cell cycle checkpoints, and the link between EXO1 and cancer.
Association between Access to Electronic Devices in the Home Environment and Cardiorespiratory Fitness in Children

This study examined the association between access to electronic devices in the home and cardiorespiratory fitness in children. Participants were children aged 8–12 years from a local elementary school (n = 106, mean age = 9.7 + 1.1 years, male = 50). Child access to electronic devices was measured with a 37-item parent-reported questionnaire. Estimated maximal aerobic capacity (VO2 Peak) was calculated from The Progressive Aerobic Cardiovascular Endurance Run (PACER) using a validated algorithm. The association between access to electronic devices in the home and cardiorespiratory fitness was explored by employing hierarchical ridge regression, using the Ordinary Least Squares (OLS) model, controlling for the covariates of sex, age, and Body Mass Index (BMI). Controlling for sex, age, and BMI, the number of electronic items in a child’s bedroom was significantly inversely related to the estimated VO2 Peak (b = −1.30 mL/kg/min, 95% C.I.: −2.46 mL/kg/min, −0.15 mL/kg/min, p = 0.028) and PACER laps (b = −3.70 laps, 95% C.I.: −6.97 laps, −0.41 laps, p = 0.028) However, the total number of electronic items in the home and total number of electronic items owned did not significantly relate to the estimated VO2 Peak (p = 0.847, 0.964) or the number of PACER laps (p = 0.847, 0.964). Child health behavior interventions focused on the home environment should devote specific attention to the bedroom as a primary locus of easily modifiable intervention.