Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence of Degree of Hydrolysis on Chemical Composition, Functional Properties, and Antioxidant Activities of Chinese Sturgeon (Acipenser sinensis) Hydrolysates Obtained by Using Alcalase 2.4L

ABSTRACT Protein hydrolysates from Chinese sturgeon were prepared using Alcalase 2.4L enzyme. Under the optimum conditions (enzyme–substrate ratio of 3.5%, pH of 8.5, and temperature of 55°C), the degree of hydrolysis (DH) was 13.8%, 16.7%, and 19.1% after 1, 3, and 6 h, respectively. The contents of crude protein and amino acid increased at DH of 19.1% to 86.97% and 78.29%, respectively. There was an obvious increase in the low-molecular-weight peptides, which could enhance the hydrolysate’s functional properties such as solubility, representing more than 90% at different pH levels. The obtained protein hydrolysates revealed good emulsification properties and high oil absorption. Furthermore, good antioxidant activities such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) inhibition, ferric reducing power, and ferrous ion (Fe2+) chelating ability were attained depending on the solution concentration. The findings indicate that the functional and antioxidant properties of protein hydrolysates could be useful in many applications of the food industry.
Utilization and Characterization of Genome-wide SNP Markers for Assessment of Ecotypic Differentiation in Arabidopsis thaliana

Single nucleotide polymorphisms (SNPs) are sequencing-based marker and very informative to explore the genetic variation that influence the phenotype (Bokharaeian et al., 2017). SNP may originated because of single nucleotide alternation (deletion, insertion or transition and transversion substitution) during evolution for adaptation International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 06 (2019) Journal homepage: http://www.ijcmas. com
Effect of Cold Plasma Treatment of Seeds on Quality of Seed Crop of Okra

The effect of cold plasma treatment of seeds on quality of seed crop of okra [Abelmoschus esculentus (L.) cultivar P-8] was studied at Department of Seed Science and Technology, Dr Y.S. Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh. The experiment comprising of seeds of two different age viz., ‘A1’ (One year old seed) and ‘A2’ (Two year old seed) and seven plasma treatments of seed viz., ‘P1’ (Only vaccum, no plasma treatment), ‘P2’ (3 minute), ‘P3’ (6 minute), ‘P4’ (9 minute), ‘P5’ (12 minute), ‘P6’ (15 minute) an ‘P7’ (Control without treatment). The seeds with different combination of treatments were evaluated for different quality parameters studied in laboratory. It was found that crop raised from one year old seeds resulted in higher germination percentage (92.39 %) in comparison with crop raised from two year old seeds (86.29 %) and the seed treated with treatment ‘P5’ shows highest germination percentage i.e. 91.75 %. This may be due to higher thousand seed weight resulted in bolder seed and have sufficient food reserve, obtaining good quality fruits which resulted in higher germination percentage. Similarly other characters like speed of germination, seedling length, seedling dry weight, SVI-I and SVI-II were found to be maximum (40.48,26.90 cm, 23.06 mg, 2486.16 and 2138.61, respectively) in one year old seeds and minimum (35.09, 26.01 cm, 21.89 mg, 2244.96 and 1890.79, respectively) in two year old seed. The reduction in seed quality parameters could be attributed to different in one year and two year old seed is due to ageing. Whereas in all the plasma treatments ‘P5’ shows highest speed of germination, seedling length, seedling dry weight, SVI-I and SVI-II. These results indicated that cold plasma treatment might promote the growth and modify the speed of germination.
Insects remove more seeds than mammals in first‐year prairie restorations

Seed sowing is a common early step in restoration, but seed consumers can impede plant establishment and alter community structure. Moreover, seed consumers vary in feeding behaviors and the relative importance of different seed consumer groups during restoration are not well understood. At 12 first‐year prairie restorations in Michigan, we studied seed predation using seed removal trays to ask: What is the relative magnitude of seed removal by insects and mammals? Do seed removal rates change over the growing season? Do habitat edges influence seed removal? At what rates are 10 prairie plant species' seeds removed by mammals and insects? Seed removal depended on consumer type, time of year, and seed species. Insects accounted for the majority of seed removal, contrary to previous research in similar systems. In May, insects removed 1.8 times more seeds than mammals, while in August, they removed 5.1 times more. There was greater seed removal in August. During May 28% of seeds were removed, compared to 54% of seeds removed during August, an increase driven by insects. Edge proximity did not influence seed removal. Certain seed species were removed more than others. For example, Lespedeza capitata (round‐headed bush clover) was always removed at high rates, whereas Coreopsis lanceolata (lance‐leaved coreopsis) and Andropogon gerardii (big bluestem) were always removed at low rates. Mammals and insects showed different preferences for several species. This research suggests a prominent role of seed predation, particularly by insects, for early prairie restoration dynamics, with influences varying temporally and among species.
Manual aspiration thrombectomy for acute and subacute inferior vena cava thrombosis and lower extremity deep venous thrombosis

Deep vein thrombosis (DVT), which can lead to pulmonary embolism (PE), is a major contributor to the global disease burden and is the third most common cardiovascular pathology after coronary artery disease and stroke. Venous thromboembolic disease, which encompasses the disease entities of DVT and PE, affects up to 10 million cases every year and represents a serious and potentially life-threatening condition. Standard anticoagulation therapy alone is ineffective at promoting deep venous system thrombus removal. Many patients develop postthrombotic syndrome (PTS) despite being on adequate anticoagulation therapy. Aggressive therapy for rapid thrombus removal is important to prevent the development of PTS. Besides impeding the onset of PTS, rapid clearance of the thrombus is also required in the treatment of phlegmasia cerulea dolens, an uncommon but life-threatening complication of acute DVT that can lead to arterial insufficiency, compartment syndrome, venous gangrene, and limb amputation. Manual aspiration thrombectomy (MAT) can provide rapid and effective therapy that could be compared to the open surgical thrombectomy approach with minimal risk of morbidity, mortality, or recurrence after surgery. Though many devices have been developed to date for pharmacomechanical thrombolysis, the cost of the treatment using these devices is very expensive. MAT is simple to perform, easy to learn, inexpensive, and rapid. This review will outline and dissect several studies and case reports, sourced from the PubMed database, on the subject of the use of MAT in treating inferior vena cava thrombosis and lower extremity DVT, including in patients with compression of the iliac vein and phlegmasia cerulea dolens.
Effects of Cranial Irradiation on Adult Hippocampal Neuronal Development and the Role of p53

Cranial radiotherapy is a standard treatment for brain tumours, but is associated with late side effects including compromised cognitive functions that can significantly impair patients’ quality of life. Cognitive impairment is a major dose-limiting factor for cranial radiotherapy, and has therefore been an important subject of study. A large body of preclinical studies suggest that injury to the hippocampus, a key structure for learning and memory, contributes to radiationinduced cognitive impairment. Hippocampus houses the life-long generation of new neurons, a process termed neurogenesis. Neurogenesis inhibition is considered to be one of the most important mechanisms leading to post-irradiation cognitive impairment. Mechanistic insights into how radiation affects neurogenesis could therefore help to alleviate the undesirable effects from cranial radiotherapy. I postulate that p53, an extensively studied tumor suppressor known to play an important role in both radiation biology and stem cell biology, regulates post-irradiation neurogenesis. The role of p53 in post-irradiation hippocampus neurogenesis was examined using transgenic mice with different copy numbers of the p53 gene. We also examined the effects of aging on postirradiation hippocampal neurogenesis because of the reported age-dependent increase of p53 in
Regulation of resection by chromatin associated proteins

The DNA molecule is permanently exposed to multiple sources of damage that threaten the genomic integrity. One of the most dangerous lesions are the Double strand breaks (DSBs), wich if unrepaired or missrepaired, can lead to several diseases including cancer. To overcome this risk, cells have evolved two main mechanisms to repair the DSBs: The Non-Homologous EndJoining (NHEJ) and the homologous recombination (HR). The balance between both repair pathways is a capital issue to ensure the proper restoration of the original DNA sequence, but regulation of this process is not fully understood. Among factors that influence the NHEJ/HR ratio, like cell cycle checkpoints or resection process, chromatin plays an important role. Chromatin is a dynamic structure that wraps and protects the DNA molecule but also modulates the accessibility of repair machinery to the lesion. In this Thesis, we selected several chromatin associated proteins to study them as possible candidates for the NHEJ/HR balance regulation. Thus, we have described a new role of RAD51 paralogs, independent of RAD51, in earlier steps of DSB repair than those described helping RAD51 in recombination. Specifically, RAD51 paralogs seem to hamper the recruitment of NHEJ machinery to resected DNA to promote the HR pathway. Moreover, the chromatin remodeler ALC1 controls resection together with eIF4A1, independently of its role as transcription factor or chromatin remodeler. Strikingly, ALC1/eIF4A1 regulates mRNA stability of CtIP, the key factor for resection promotion. Lastly, we have reported a nuclear direct role of ELP6 protein in the DSB repair that is not influenced by the histone acetyltransferase (HAT) activity of transcription elongator complex.
Towards a Refined Inventory of Lexical Bundles: an Experiment in the Formulex Method

A number of corpus studies focusing on the description of the use and functions of lexical bundles havebeen conducted recently in order to explore the phraseology of learner language. As with any studiesof lexical bundles, the problem of overlapping or structurally incomplete items poses a particularchallenge. In practice, it is often difficult to align such units with specific discourse functions. The factthat lexical bundles do not constitute neat form-and-meaning mappings results from, among otherreasons, their being grounded in language use rather than language system. In this pilot study weattempt to test a new method called Formulex (Forsyth, 2015a; 2015b) to verify whether an applicationof the criterion of coverage – in addition to the conventional criteria of orthographic length, minimumfrequency and distribution range (Biber et al., 1999) – may help obtain a more refined inventory of lexicalbundles and hence facilitate further qualitative analyses. To that end, we use Polish and Lithuaniancomponents of the International Corpus of Learner English (ICLE, Granger et al., 2009), as well as theLOCNESS corpus (CECL), representing academic essays written by British and American students. Theresults revealed that many lexical bundles of fixed length identified in a conventional way are fragmentsof longer chunks of text and hence they should not be treated as complete or standalone 4-word lexicalitems. It was also revealed that the application of the Formulex method, where the word sequences aremutually exclusive, helps a researcher filter out overlapping or non-perceptually salient lexical bundlesand, ultimately, specify more precise boundaries of lexical bundles of fixed length. DOI: http://dx.doi. org/10.5755/j01.sal. 0.29. 15327
Content-based Retrieval of 3D Medical Images

Abstract -- While content-based image retrieval (CBIR) has been researched for more than two decades, retrieving 3D datasets has been progressing considerably more slowly, especially in respect to its application to the medical domain. This is in part due to the limitation of processing speed when trying to retrieve high-resolution datasets in real-time. Another barrier is that most existing methods have been developed based on 2D images instead of 3D, leaving a gap to be filled. At present, a significant number of exploitations are focusing on the extraction of 3D shapes. However, it appears other information tends to be equally important in clinical decision making. In this paper, Local Binary Pattern (LBP), a texture based approach stemming from 2D forms, has been studied extensively through the application to 3D images from a collection of MR brain images in a content-based image retrieval system (CBIR). The initial results show LBP not only can achieve a precision rate of up to 78% but also can perform retrieval in real time with sub-second processing speeds. Comparison with the other three popular texture-based methods, namely 3D Grey Level Co-occurrence Matrices, 3D Wavelet Transforms and 3D Gabor Transforms, is also carried out. The results demonstrate that LBP outperforms them all in terms of retrieval precision and processing speed. Keywords – CBIR, 3D image retrieval, 3D texture extraction.
Risk of metastatic disease on 68gallium‐prostate‐specific membrane antigen positron emission tomography/computed tomography scan for primary staging of 1253 men at the diagnosis of prostate cancer

To determine the number of men with 68gallium‐prostate‐specific membrane antigen positron emission tomography/computed tomography (68Ga‐PSMA PET/CT) avid metastasis at diagnosis, as most data on 68Ga‐PSMA PET/CT are for the evaluation of recurrent disease after primary treatment and to our knowledge this study is the largest series of primary prostate cancer staging with 68Ga‐PSMA PET/CT.
Steady-state responses in the somatosensory system interact with endogenous beta activity

Brain oscillations have been related to many aspects of human behavior. To understand a potential causal relationship, it is of great importance to develop methods for modulating ongoing neural activity. It has been shown that external rhythmic stimulation leads to an oscillatory brain response that follows the temporal structure of the presented stimulus and is assumed to reflect the synchronization of ongoing neural oscillations with the stimulation rhythm. This interaction between individual brain activity and so called steady-state evoked potentials (SSEPs) is the fundamental requirement of neural entrainment. Here, we investigate whether neural responses to rhythmic vibrotactile stimulation, measured with EEG, are dependent on ongoing individual brain oscillations, and therefore reflect entrained oscillatory activity. For this, we measured phase synchronization in response to rhythmic stimulation across various frequencies in the alpha and beta band. Three different stimulation intensities were applied for each frequency relative to the individual sensory threshold. We found that a higher stimulation intensity, compared to lower intensities, resulted in a more pronounced phase synchronization with the stimulation signal. Moreover, EEG responses to low stimulation frequencies closer to individual beta peak frequencies revealed a higher degree of entrainment, compared to stimulation conditions with frequencies that were more distant to endogenous oscillations. Our findings provide evidence that the efficacy of vibrotactile rhythmic beta stimulation to evoke a SSEPs is dependent on ongoing brain oscillations.
Long-term ophthalmologic examinations of eyes with equine recurrent uveitis after pars plana vitrectomy

Equine recurrent uveitis (ERU) is an ocular disease in horses of different breeds with a prevalence between 2.7 and 7.6 % in Europe and vitrectomy is a common method of treatment. The objective of the study was to evaluate the long-term retention of vision and control of uveitis after pars plana vitrectomy performed between 2005 and 2013 on eyes with a history of equine recurrent uveitis (ERU). Vitrectomy in eyes with ERU can influence the recurrent nature of the disease. Previous studies indicated success rates regarding the further absence of uveitis between 73.6 and 97.7 %. Owners of the horses were contacted and an ophthalmological reexamination including tonometry, induction of mydriasis, slit lamp biomicroscopy and direct ophthalmology was performed in 116 horses (147 eyes vitrectomized). Visual acuity was examined by the dazzle reaction and the menace response. Aspects such as gender, age and breed of the horses, as well as leptospiral status and preexisting damage of operated eyes are described. A total of 246 eyes were accessible for follow-up information, whereas 147 eyes were available for ophthalmologic reexaminations. If only the reexamined eyes are considered, an improvement of the condition was seen in 133 eyes (91 %) and 14 eyes (9 %) showed at least one uveitic recurrence after vitrectomy. A positive menace response and a positive dazzle reaction were observed in 128 of the 147 reexamined eyes (87 %). The number of episodes prior to vitrectomy, the gender of the horse and the leptospiral status of the eye showed no significant correlation with the prevalence of recurrences. Coat color, breed and age of the horse operated, and preexisting ocular alterations showed a significant influence on the chance of postoperative recurrences of uveitis. Horses with a coat color other than “chestnut” and “black and brown” had significantly more recurrences and odds ratio for these horses developing recurrences was also significantly higher. The present study shows that a large number of reexamined eyes remained visual (87 %) and a large proportion of the eyes (91 %) showed an improvement of the condition after vitrectomy. Results of this study suggest that genetic factors, age and preexisting ocular alterations influence the long-term prognosis of eyes with ERU after vitrectomy.
QTL MAPPING OF ESTERASE ISOZYME FORMS IN Brassica rapa L. MATURE SEEDS

loci responsible for the activity of 13 different esterase forms of mature seeds in L. identified mapped for the time. The doubled haploid lines of two mapping populations, DH30 and DH38, were studied. All identified esterase isoforms were divided into three groups according to their electrophoretic mobility. The group of isoforms A1-A3 had a high molecular weight and low electrophoretic mobility. The group of B1-B7 isoforms exhibited an average molecular weight and an average electrophoretic mobility. The C1-C3 group consisted of isoforms having a low weight and, the highest electrophoretic mobility. QTL analysis loci esterase isoform composite interval mapping QTLs on esterase isoforms population isoform for the DH30 two isoforms, analysis initial these loci in the ontogenesis and adaptability of plants B. rapa . In general, the carried out mo- lecular genetic mapping and biochemical analysis of the studied biochemical traits of various manifestation of esterase isoforms in mature seeds of B. rapa revealed genetic determinants of the studied characters, as well as the genome distribution of mapped QTLs, which in the long term makes it possible to conduct effective molecular and genetic screening of collection accessions and breeding material of the B. rapa species according to these biochemical characters when perform-ing genetic and selection investigations in this species.
Epigenetic plasticity potentiates a rapid cyclical shift to and from an aggressive cancer phenotype

Highly tumorigenic, drug‐resistant cancer stem‐like cells drive cancer progression. These aggressive cells can arise repeatedly from bulk tumor cells independently of mutational events, suggesting an epigenetic mechanism. To test this possibility, we studied bladder cancer cells as they cyclically shifted to and from a cancer stem‐like phenotype, and we discovered that these two states exhibit distinct DNA methylation and chromatin accessibility. Most differential chromatin accessibility was independent of methylation and affected the expression of driver genes such as E2F3, a cell cycle regulator associated with aggressive bladder cancer. Cancer stem‐like cells exhibited increased E2F3 promoter accessibility and increased E2F3 expression that drove cell migration, invasiveness and drug resistance. Epigenetic interference using a DNA methylation inhibitor blocked the transition to a cancer stem‐like state and reduced E2F3 expression. Our findings indicate that epigenetic plasticity plays a key role in the transition to and from an aggressive, drug‐resistant phenotype.
Exogenous Protein as an Environmental Stimuli of Biofilm Formation in Select Bacterial Strains

A screening of environmental conditions that would elicit robust biofilm in a collection of Serratia marcescens isolated from soil revealed that exogenous milk protein increased biofilm productivity up to ten-fold. A select screening of fish pathogens, freshwater and human isolates identified several other species that responded similarly to exogenous protein. The optimal protein concentration was species specific; S. marcescens at 5% milk protein, Aeromonas sp. at 2-3%, Flavobacterium columnare at 1% and Pseudomonas aeruginosa at 0.1-0.4%. Media supplemented with milk protein also increased the cell counts in biofilm as well as the protein incorporated into the biofilm matrix. These data suggest that relatively high concentrations of exogenous protein may serve as an environmental trigger for biofilm formation, particularly for pathogenic bacteria exposed to relatively high concentrations of protein in bodily fluids and mucosal surfaces.
A multimodal investigation of stress effects on memory processes

Stress verbessert das Gedachtnis besonders fur zentrale Objekte nach 24 Stunden. In einer Eye Tracking Studie wurde mittels Trier Sozial-Stress Test untersucht, ob Stress die Fixation zentraler Objekte beeinflusst, und sich das Gedachtnis dadurch verbessert. In der 2. Studie wurde erhohte Vigilanz durch Stress nach 24 Stunden uber den Auditorischen Startle Reflex unter Einbezug eines Duftes untersucht. Die 3. Studie behandelte direkte Stress Effekte auf Gedachtnis und Startle Reflex. Stress fuhrte zu langerer Fixation, die jedoch nicht mit verbessertem Objektgedachtnis korrelierte. Hypervigilanz durch Stress fuhrte zu starkerem Startle Reflex am nachsten Tag ohne verstarkte Spezifizitat in Bezug auf den Duft aus der Stresssituation. Direkt nach der Stresserfahrung zeigte sich tendenziell das gegenteilige Muster. Das Objektgedachtnis war bereits verbessert. Die Befunde tragen zu einem besseren Verstandnis behavioraler und physiologischer Kurz- und Langzeiteffekte von Stress bei.