Miracle-dz/UniFAR-FTUs huggingface dataset:
Enzyme-Linked Immunosorbent Assays Using Recombinant Envelope Protein Expressed in COS-1 and Drosophila S2 Cells for Detection of West Nile Virus Immunoglobulin M in Serum or Cerebrospinal Fluid

ABSTRACT Humans infected with West Nile virus (WNV) develop immunoglobulin M (IgM) antibodies soon after infection. The microtiter-based assays for WNV IgM antibody detection used by most state public health and reference laboratories utilize WNV antigen isolated from infected Vero cells or recombinant envelope protein produced in COS-1 cells. Recombinant antigen produced in COS-1 cells was used to develop a WNV IgM capture enzyme immunoassay (EIA). A supplementary EIA using WNV envelope protein expressed in Drosophila melanogaster S2 cells was also developed. Both assays detected WNV IgM in the sera of experimentally infected rhesus monkeys within approximately 10 days postinfection. Human sera previously tested for WNV IgM at a state public health laboratory (SPHL) were evaluated using both EIAs. Among the sera from 20 individuals with laboratory-confirmed WNV infection (i.e., IgM-positive cerebrospinal fluid [CSF]) that were categorized as equivocal for WNV IgM at the SPHL, 19 were IgM positive and one was negative by the new EIAs. Of the 19 IgM-positive patients, 15 were diagnosed with meningitis or encephalitis; the IgM-negative patient was not diagnosed with neurological disease. There was 100% agreement between the EIAs for the detection of WNV IgM. CSF samples from 21 individuals tested equivocal for WNV IgM at the SPHL; all 21 were positive in both bead assays, and 16 of these patients were diagnosed with neurological disease. These findings demonstrate that the new EIAs accurately identify WNV infection in individuals with confirmed WNV encephalitis and that they exhibit enhanced sensitivity over that of the microtiter assay format.
Geophysical Variables and Behavior: LXXXII. A Strong Association between Sudden Infant Death Syndrome and Increments of Global Geomagnetic Activity—Possible Support for the Melatonin Hypothesis

A significant subpopulation of young infants who die suddenly (SIDS) often exhibit anomalies consistent with disruptions within the pineal-limbic system. We have hypothesized that sudden decreases in nocturnal melatonin by a specific range of geomagnetic activity would precipitate sudden infant death. A correlation of . 90 was found between the numbers of cases of Sudden Infant Death Syndrome per month during the years 1960 and 1961 in Ontario and an increase (primarily) in numbers of days per month with average geomagnetic activity between 11 and 20 nT and 31 through 40 nT but a decrease in the numbers of days with values between 21 and 30 nT. This nonlinear sensitivity, presumably associated with specific ripple frequencies within the geomagnetic field, may explain the failure by other researchers to detect linear associations between the numbers of these types of deaths and gross, scalar indicators of geomagnetic activity.
Collective behavior of swarms with general nonlinear attraction and repulsion functions

WE study the swarms (multi-agent systems in network) with general nonlinear attraction and repulsion functions. Our work generalizes the results given by Gazi and Passino [7] and Chu et al. [9, 11] into a more general setting. It is shown that the members of multi-agents system in reciprocal communication network will asymptotically form a cohesive cluster with finite size if the nonlinear attraction and repulsion functions satisfy some very mild assumptions. These results can be generalized into the case that the network is non-reciprocal with detailed balance condition. However, under general non-reciprocal networks the members of the system could appear some oscillatory or dispersed behavior depending on the choices of the nonlinear attraction and repulsion functions. We present several numerical simulations demonstrating the correctness of our theoretic results.
Intravenous flumazenil versus oxazepam tapering in the treatment of benzodiazepine withdrawal: a randomized, placebo‐controlled study

Flumazenil (FLU), a benzodiazepine (BZD) partial agonist with a weak intrinsic activity, was previously found unable to precipitate withdrawal in tolerant subjects submitted to long‐lasting BZD treatment. The potential use of FLU to treat BZD withdrawal symptoms has also been evaluated tentatively in clinical studies. In the present experiment, FLU (treatment A) was compared with oxazepam tapering (treatment B) and placebo (treatment C) in the control of BZD withdrawal symptoms in three groups of BZD dependent patients. Group A patients (20) received FLU 1 mg twice a day for 8 days, and oxazepam 30 mg in two divided doses (15 mg + 15 mg) during the first night, oxazepam 15 mg during the second night and oxazepam 7.5 mg during the third night. FLU was injected i.v. in saline for 4 hours in the morning and 4 hours in the afternoon, in association with placebo tablets. Group B patients (20) were treated by tapering of oxazepam dosage (from 120 mg) and with saline solution (as placebo) instead of FLU for 8 days. Group C patients (10) received saline instead of FLU and placebo tablets instead of oxazepam for 8 days. FLU immediately reversed BZD effects on balance task and significantly reduced withdrawal symptoms in comparison with oxazepam and placebo on both self‐reported and observer‐rated withdrawal scales. The partial agonist also reduced craving scores during the detoxification procedure. In addition, during oxazepam tapering, group B patients experienced paradoxical symptoms that were not apparent in FLU patients. Patients treated with FLU showed a significantly lower relapse rates on days 15, 23 and 30 after the detoxification week. Our data provide further evidence of FLUs ability to counteract BZD effects, control BZD withdrawal and normalize BZD receptor function. The effectiveness of FLU may reflect its capacity to upregulate BZD receptors and to reverse the uncoupling between the recognition sites of BZD and GABA, on the GABA A macromolecular complex, that has been reported in tolerant subjects.
A Computer-Based Interactive System for Group Decisionmaking

The rationale, design, and operation of the Perceptronics' Group Decision Aid, are described, and some experimental studies of its effectiveness are reported. The decision aid is supported by a PDP 11/45 minicomputer using the UNIX operating system. It features simple individual data entry terminals and a large screen (7 ft) color video display for feedback of computer-generated information. The system's purpose is to guide the group decisionmaking process by selective elicitation of a decision tree which incorporates value and probability inputs from all group members. Conflicts in value judgments are identified and discussions are initiated through use of multiattribute utility analysis. A specially trained system operator, called an intermediator, facilitates group interaction with the aiding program so that group members need have no prior familiarity with computers or decision analysis. Experimental studies are described comparing the performance, behavior, and responses of aided and nonaided groups in a complex international crisis decision problem. Improvements with aiding were noted in the completeness of the decision process and in the satisfaction and confidence of the group participants.
Specific Personality Traits and Coping Styles Predict Affective Symptoms in Early Post Acute Coronary Syndrome Inpatients

Objective: Affective symptoms have adverse effects in hospital and long-term cardiac outcomes of post Acute Coronary Syndrome (ACS) patients. This study aims to identify personality traits and maladaptive coping strategies that could predict affective symptoms in early post-ACS patients. Methods: Seventy patients undergoing revascularization procedures were examined within a week after their admission by means of the Hospital Anxiety-Depression Scale. Personality was analyzed through the Type D Personality Scale and the Coping Inventory for Stressful Situation. Multiple logistic regression analysis was used to determine independent predictors of anxiety and depressed mood. Results: A high rate of depressive and anxious symptoms was found and 76% of patients resulted Type D personality. Depression was associated with b-blocker therapy, Type D personality, and specific coping strategies. Unmarried status, low education, unstable angina, Type D personality, emotion, and avoidance oriented coping independently predicted anxiety. Conclusion: These findings underlie the importance of assessment for Type D personality and coping strategies that could be useful to identify post-ACS patients at higher risk for affective symptoms. Using these brief instruments, as sensitive screening measures, we investigated the prevalence of depressive and anxiety symptoms in patients with ACS, we identified personality traits and coping strategies used to manage stress and estimated independent predictors of affectivity disorders after ACS.
Understanding design patterns — what is the problem?

Design patterns codify proven solutions to recurring design problems. Their proper use within a development context requires that: (i) we understand them; (ii) we ascertain their applicability or relevance to the design problem at hand; and (iii) we apply them faithfully to the problem at hand. We argue that an explicit representation of the design problem solved by a design pattern is key to supporting the three tasks in an integrated fashion. We propose a model‐driven representation of design patterns consisting of triples < MP, MS, T > where MP is a model of the problem solved by the pattern, MS is a model of the solution proposed by the pattern, and T is a model transformation of an instance of the problem into an instance of the solution. Given an object‐oriented design model, we look for model fragments that match MP (call them instances of MP), and when one is found, we apply the transformation T yielding an instance of MS. Easier said than done. Experimentation with an Eclipse Modeling Framework‐based implementation of our approach applied to a number of open‐source software application's raised fundamental questions about: (i) the nature of design patterns in general, and the ones that lend themselves to our approach, and (ii) our understanding and codification of seemingly simple design patterns. In this paper, we present the principles behind our approach, report on the results of applying the approach to the Gang of Four (GoF) design patterns, and discuss the representability of design problems solved by these patterns. Copyright © 2011 John Wiley & Sons, Ltd.
Toward Understanding Changes in Perceptions of Assessment for Student Affairs Professionals: A Case-Study Approach

As national and institutional pressures to adopt reporting student success grow, understanding these changes and the impact on our staff is important. This case study looked at a student affairs division at a large urban university in the southwest to better understand the changes of perceptions of assessment. The themes found were: 1. ) assessment was not to be feared, 2. ) SAD was contributing to the larger campus voice, 3. ) assessment was a must have for departments, and 4. ) good professionals use assessment. The implications toward practice in a culture of assessment and future research directions are discussed.
[VERTEBRAL SUBCHONDRAL BONE AND INTERVERTEBRAL DISC DEGENERATION].

OBJECTIVE To review the role of vertebral subchondral bone in maintaining normal physiological function of the intervertebral disc and in the intervertebral disc degeneration in light of bone anatomy, microstructure, histopathological features, and MRI imaging features. METHODS The related home and abroad literature concerning vertebral subchondral bone and intervertebral disc degeneration was extensively reviewed and comprehensively analyzed. RESULTS Vertebral subchondral bone is part of the vertebral endplate and is defined as the vascularized cortical and trabecular bone layer located between the cartilage endplate and vertebral body. It not only plays a cushion shocks role in terms of conducting stress and effectively resists the hydrostatic nucleus, but also ensures the normal supply of disc nutrition. Subchondral bone sclerosis caused by bone remodeling abnormality severely decreases the ability of subchondral bone stress absorption and protective function of disc, which finally leads to increased inflammatory factors locally and hindered nutrition pathway of disc and enhanced disc degeneration. CONCLUSION To further strengthen the knowledge and understanding of the vertebral subchondral bone will play a positive role in the study on the pathogenesis of intervertebral disc degeneration.
Postoperative myocardial infarction after diagnostic video-assisted thoracoscopy and pleurodesis for catamenial pneumothorax: A unique case report

Myocardial infarction (MI) is uncommon in patients undergoing noncardiac surgery without a history of coronary artery disease. But, patients with compromised pulmonary function and coexisting anaemia superimposed by precipitating factors like prolonged hypotension and tachycardia can culminate in myocardial catastrophe even in the absence of risk factors. We are herewith reporting an unusual case of postoperative non-ST elevation MI without any pre-existing ischemic heart disease. A 39-year-old female patient who was submitted for diagnostic video-assisted thoracoscopy and chemical pleurodesis for recurrent pneumothorax developed postoperative MI. After review of all the factors, it was found that the patient developed Type 2 MI as a sequel to oxygen supply and demand mismatch secondary to hypoxia and prolonged hypotension. This was evident in the 12-lead electrocardiogram and was confirmed by elevated cardiac biomarkers and regional wall motion abnormality on echocardiography.
Hedonic bull pricing models: estimating the value of traits of bulls sold following performance testing

Selection of a herd sire has always been of paramount importance given the initial financial investment and their contribution and effect on the genetic make-up of a beef herd. Data was collected from the nation’s longest consecutively run bull test conducted at the University Farm of Oklahoma Panhandle State University (OPSU). The Bull Test and Bull Sale data utilized were collected from 2008-2013. Performance data was collected over a 112 day test period with data collection occurring at 28 day intervals. The top seventy bulls from each year’s test were selected based upon a performance index of 1⁄2 ADG and 1⁄2 weight per day of age (WDA), and a semen quality and motility score of excellent and sold at auction. Angus bulls were the focus of the study as they represented the vast majority of individuals sold. Three hedonic pricing models were created to try to determine what attributes buyers at the OPSU bull test sale were placing emphasis on. The initial hedonic model contained production data that included BW, ADG, WDA, Julian age, final test weight ultrasound data, and a dummy variable for sale year. The second model utilized all production data and added genetic variables in the form of production EPDs (Calving Ease Direct (CED), BW, Weaning Weight and Yearling Weight) and maternal EPDs (Calving Ease Maternal, Maternal Milk). The third model included the variables from the first and second models with the inclusion of carcass EPDs (Marbling, Ribeye Area (REA) and FAT). Year was significant in all three models however there was less of an effect on price as more variables were included. In model one, the production facts that were of significance were: ADG (P<0.01), BW (P<0.01) and final test weight (P<0.01). In the second model, ADG, BW and final test weight retained their significance at the P<0.01 level. The only production EPD that was significant (P<0.05) was CED. In the third model, years, ADG and BW were still significant (P<0.01). Final test weight (P=0.70) and CED (P = 0.132) had substantial changes. The carcass EPD ribeye area had a P value of 0.057. Producers who are placing bulls on test can utilize the given information to assist with their selection. It cannot go unsaid that while single trait selection can be very detrimental; ADG was significant across all models. The study indicates that performance and growth are of utmost importance to buyers, followed by birth weight
Insulin-like growth factor-II and insulin-like growth factor binding protein-2 prospectively predict longitudinal elevation of HDL-cholesterol in type 2 diabetes

Introduction Associations of insulin-like growth factor-II (IGF-II) and insulin-like growth factor binding protein-2 (IGFBP-2) with cardiovascular risk have been inadequately studied. We hypothesized that IGF-II and IGFBP-2 associate with longitudinal trends in lipid profiles in type 2 diabetes patients. Subjects and methods Four hundred and eighty nine subjects with type 2 diabetes (age 27–87 years) from the Salford Diabetes Cohort were studied. Longitudinal clinical information was extracted for an eight-year period (2002–2009) from an integrated electronic dataset of primary care and hospital data. Results There were 294 male subjects and mean age was 62.9 years. At baseline, IGF-II concentration was 602 ng/mL. HDL cholesterol at baseline was associated with log-IGF-II concentration in a model adjusted for age, gender, baseline body-mass index (BMI), estimated glomerular filtration rate (eGFR) and lipid-lowering therapy. IGFBP-1 and IGFBP-2 were associated with high HDL-cholesterol. A higher circulating IGF-II concentration at baseline was also associated with longitudinal increase in HDL-cholesterol in mixed-effects regression analyses independent of IGF-I, IGFBP-1, IGFBP-2, IGFBP-3, age, gender, eGFR, BMI and lipid-lowering therapy. Log-transformed baseline concentrations of IGFBP-1 and IGFBP-2 were also associated with longitudinal elevation in HDL-cholesterol. No association was observed for IGF-II or IGFBP-2 with longitudinal LDL cholesterol trends. Conclusion Our analyses based on ‘real world’ data demonstrate that higher baseline IGF-II and IGFBP-2 predict increased HDL concentration over time, implicating IGF-II in modulation of circulating HDL-cholesterol concentrations.
An Investigation of Preservice Teachers’ Knowledge of Diversity and Multicultural Literature at One University

The research study surveyed students classified as Juniors at the University of Arkansas currently enrolled in the Children’s Literature course, a required class for both Elementary Education and Childhood Education degree program students attending the University of Arkansas. The survey that was administered is the Cultural Diversity Awareness Inventory (CDAI). Additional data collected consisted of observations and interviews of students enrolled in the course. The research lasted one semester and consisted of multiple components. Through the study, the data suggests students grew in their knowledge of multicultural concepts and multicultural literature, but their growth was better measured through interviews, not the CDAI survey. AN INVESTIGATION OF PRESERVICE TEACHERS 3
Modeling & Analysis of Design Parameters for Portable Hand Orthoses to Assist Upper Motor Neuron Syndrome Impairments and Prototype Design

Wearable assistive robotics have the potential to address an unmet medical need of reducing disability in individuals with chronic hand impairments due to neurological trauma. Despite myriad prior works, few patients have seen the benefits of such devices. Following application experience with tendon-actuated soft robotic gloves and a collaborator’s orthosis with novel flat-spring actuators, we identified two common assumptions regarding hand orthosis design. The first was reliance on incomplete studies of grasping forces during activities of daily living as a basis for design criteria, leading to poor optimization. The second was a neglect of increases in muscle tone following neurological trauma, rendering most devices non-applicable to a large subset of the population. To address these gaps, we measured joint torques during activities of daily living with able-bodied subjects using dexterity representative of orthosis-aided motion. Next, we measured assistive torques needed to extend the fingers of individuals with increased flexor tone following TBI. Finally, we applied this knowledge to design a cable actuated orthosis for assisting finger extension, providing a basis for future work focused on an under-represented subgroup of patients.
Altered frequency of type 1 cytokine secreting cells in the peripheral blood of patients with primary Sjögren's syndrome.

OBJECTIVE An imbalance in immunoregulatory cytokines may contribute to the etiopathogenesis of Sjogren's syndrome (SS). We investigated systemic abnormalities in cytokine production in the peripheral blood in patients with SS. METHODS ELISPOT assays were used to detect and enumerate cells spontaneously secreting interleukin 2 (IL-2), IL-6, IL-10, and interferon-gamma (IFN-gamma) in freshly isolated peripheral blood mononuclear cells from 20 patients with SS and 20 healthy controls. RESULTS The number of cells spontaneously secreting type 1 cytokines IL-2 and IFN-gamma was decreased in the peripheral blood of patients with SS compared to controls. There was no change observed in the number of cells spontaneously secreting IL-6 and IL-10. Cells spontaneously secreting IL-4 were too rare in peripheral blood to evaluate, although cells capable of secreting IL-4 in response to phytohemagglutinin did not differ from controls. Patients with severe extraglandular symptoms (such as vasculitis) had a significantly lower frequency of IFN-gamma secreting cells in their peripheral blood than those without extraglandular involvement. CONCLUSION These results suggest that decreased type 1 cytokine production may contribute to or reflect the pathogenesis of SS.
Protein-tyrosine Pseudokinase 7 (PTK7) Directs Cancer Cell Motility and Metastasis*

Background: In embryonic development, PTK7 regulates orientation of cells in a tissue plane. Results: PTK7 controls cellular protrusions and, as a result, directional cell motility and metastasis in fibrosarcoma HT1080 cells. Conclusion: Both PTK7 expression and proteolysis contribute to efficient cell motility and metastasis. Significance: PTK7 is a potential diagnostic biomarker with predictive value and a promising drug target in cancer. It is well established that widely expressed PTK7 is essential for vertebrate tissue morphogenesis. In cancer, the functionality of PTK7 is selectively regulated by membrane type-1 matrix metalloproteinase (MT1-MMP), ADAMs (a disintegrin domain and metalloproteinases), and γ-secretase proteolysis. Here, we established that the full-length membrane PTK7, its Chuzhoi mutant with the two functional MT1-MMP cleavage sites, and its L622D mutant with the single inactivated MT1-MMP cleavage site differentially regulate cell motility in a two-dimensional versus three-dimensional environment. We also demonstrated that in polarized cancer cells, the levels of PTK7 expression and proteolysis were directly linked to the structure and kinetics of cell protrusions, including lamellipodia and invadopodia. In the functionally relevant and widely accepted animal models of metastasis, mouse and chick embryo models, both the overexpression and knock-out of PTK7 in HT1080 cells abrogated metastatic dissemination. Our analysis of human tissue specimens confirmed intensive proteolysis of PTK7 in colorectal cancer tumors, but not in matching normal tissue. Our results provide convincing evidence that both PTK7 expression and proteolysis, rather than the level of the cellular full-length PTK7 alone, contribute to efficient directional cell motility and metastasis in cancer.