Miracle-dz/UniFAR-FTUs huggingface dataset:
Phylogenomics supports incongruence between ecological specialization and taxonomy in a charismatic clade of buck moths

Local adaptation can be a fundamental component of speciation, but its dynamics in relation to gene flow are not necessarily straightforward. Herbivorous taxa with localized host plant or habitat specialization across their geographic range are ideal models for investigating the patterns and constraints of local adaptation and its impact on diversification. The charismatic, day‐flying moths of the Hemileuca maia species complex (Lepidoptera: Saturniidae) are such taxa, as they are geographically widespread, exhibit considerable ecological and morphological variability and host and habitat specificity, but apparently lack genetic differentiation across their range. Here, we use genomewide single nucleotide polymorphisms to assess relationships and population structure of this group across North America and investigate the scales where genomic divergence correlates with adaptive ecological characteristics. In contrast to previous genetic studies of the group, we find broad‐ and fine‐scale genetic differentiation between lineages, which is at odds with various levels of taxonomic description and recognition of conservation units. Furthermore, ecological specialization only explains some fine‐scale genetic differentiation, and across much of the group's range, local adaptation is apparently occurring in the face of strong gene flow. These results provide unprecedented insight into drivers of speciation in this group, the relationship between taxonomy and genomics‐informed species boundaries and conservation management of internationally protected entities. Broadly, this system provides a model for understanding how local adaptation in an herbivore can arise and be maintained in the face of apparently strong gene flow, and the importance of geographic isolation in generating genomic divergence, despite a lack of ecological divergence.
Multi-Criteria Decision Making for Optimal Configuration of Business Process Model Families

Business process model families (BPMF) provide collective representation of business processes that can be defined for a target domain of interest. They enable rapid development of appropriate business processes by quick BPMF configuration based on the stakeholders’ needs and preferences. In this paper we propose a novel framework for user-centric quality-driven configuration of business process model families capturing different kinds of non-functional preferences and solving the optimal BPMFs configuration problem. Focusing on the following key elements: Analytic Hierarchy Process (AHP), and its extension known as CS-AHP, Fuzzy logic and Genetic Algorithms; the framework maximizes the degree of satisfaction of defined non-functional preferences while simultaneously preserving the behavioural correctness of each derived business process. Furthermore, we provide extensive analyses of the proposed framework from three different perspectives: accuracy assessment, analysis of the impact of different variability and uncertainty elements, and comparison with respect to the published literature. Empirical analyses in this paper indicated that the proposed approach outperforms competitors. DOI: http://dx.doi. org/10.5755/j01.itc. 47.3. 18652
Perinatal Maternal Stress and Susceptibility to Infectious Diseases in Later Childhood: An Early Life Programming Perspective

Abstract There is evidence of transmission of stress-related dysregulation from parents to offspring during early developmental stages, leading to adverse health outcomes. This study investigates whether perinatal stress is linked to the risk of infectious diseases in children aged 7–11 years. We hypothesize that stress exposure during pregnancy and the first 6 months after birth independently predict common infectious diseases. Data are obtained from ELSPAC-CZ, a prospective birth cohort. Maternal stress, operationalized as the number of life events, is examined for pregnancy and the first 6 months postpartum. Children’s diseases include eye infection, ear infection, bronchitis/lung infection, laryngitis, strep throat, cold sores, and flu/flu-like infection. More prenatal and postnatal life events are both independently linked to a higher number of infectious diseases between the ages of 7–11 years. The effect is larger for postnatal vs. prenatal events, and the effect of prenatal events is attenuated after maternal health in pregnancy is controlled. The results suggest that perinatal stress is linked to susceptibility to infectious diseases in school-age children. Interventions to address stress in pregnant and postpartum women may benefit long-term children’s health.
So much more than a necessary evil? Lessons from a study following the implementation of recognition of trainers

Background: The UK General Medical Council (GMC) now requires that trainers in specified roles be "recognised" by evidencing training (General Medical Council, 2012), with wide variation across the UK in implementation. This study aimed to understand the impact on established local educators of this mandatory requirement but also to investigate how our faculty development programme (the Clinical Educator Programme – CEP) can optimise this process for future trainers. Literature review found little published work on the Framework Standards used for this process. Methods: From a pragmatic stance, case study methodology was used allowing for mixed methods of data collection (online survey and focus groups) and "insider research" (Unluer, 2012). Consultants holding a named role (n=875) as defined by the GMC (2012, p.15) were sampled. Results: Of the 28% who responded, the majority (83.3%) were clinical supervisors (n=204). 77.6% were registered on the Faculty Development Programme (CEP, n=190), most stating they registered because of Recognition of Trainers (RoT) (n=107). Demonstrable differences were found between CEP participants and others and between those registered for personal developmental reasons when compared to those who gave RoT as their only reason. This included potential benefits and risks of the process. Ten themes emerged including "a necessary evil". Support for CEP contributing to a community of practice (Lave & Wenger, 1991) was found. Mandatory requirements should not detract from professionalization of medical education, yet this study found just over half of respondents were supportive of the implemented process (51.8%). Conclusion: Further support is needed to help clinical educators understand the potential benefits of on-going educational CPD. Crichton F, Jones L MedEdPublish https://doi.org/10.15694/mep.2018. 0000073.1 Page | 2
Effect of Foster Care Intervention on Trajectories of General and Specific Psychopathology Among Children With Histories of Institutional Rearing: A Randomized Clinical Trial

Importance It is unclear whether early institutional rearing is associated with more problematic trajectories of psychopathology from childhood to adolescence and whether assignment to foster care mitigates this risk. Objectives To examine trajectories of latent psychopathology factors—general (P), internalizing (INT), and externalizing (EXT)—among children reared in institutions and to evaluate whether randomization to foster care is associated with reductions in psychopathology from middle childhood through adolescence. Design, Setting, and Participants This longitudinal, intent-to-treat randomized clinical trial was conducted in Bucharest, Romania, where children residing in 6 institutions underwent baseline testing and were then randomly assigned to a care as usual group (CAUG) or a foster care group (FCG). A matched sample of a never-institutionalized group (NIG) was recruited to serve as a comparison group. The study commenced in April 2001, and the most recent (age 16 years) follow-up started in January 2015 and is ongoing. Intervention Institutionally reared children randomized to high-quality foster homes. Main Outcomes and Measures Psychopathology was measured using the MacArthur Health and Behavior Questionnaire. Teachers and/or caregivers reported on symptoms of psychopathology in several domains. Results A total of 220 children (50.0% female; 119 ever institutionalized) were included in the analysis at the mean ages of 8, 12, and 16 years. A latent bifactor model with general (P) and specific internalizing (INT) and externalizing (EXT) factors offered a good fit to the data. At age 8 years, CAUG (mean, 0.41; 95% CI, 0.17-0.67) and FCG (mean, 0.30; 95% CI, 0.04-0.53) had higher P than NIG (mean, −0.40; 95% CI, −0.56 to −0.18). By age 16 years, FCG (mean, 0.07; 95% CI, −0.18 to 0.29) had lower P than CAUG (mean, 0.37; 95% CI, 0.13-0.60). This effect was likely driven by modest declines in P from age 8 years to age 16 years among FCG (slope, −0.12; 95% CI, −0.26 to 0.04) compared with CAUG, who remained stably high over this period (slope, −0.02; 95% CI, −0.19 to 0.14). Moreover, CAUG and FCG showed increasing divergence in EXT over time, such that FCG (mean, −0.30; 95% CI, −0.58 to −0.02) had fewer problems than CAUG (mean, 0.05; 95% CI, −0.25 to 0.36) by age 16 years. No INT differences were observed. Conclusions and Relevance Institutionalization increases transdiagnostic vulnerability to psychopathology from childhood to adolescence, a period of significant social and biological change. Early assignment to foster care partially mitigates this risk, thus highlighting the importance of social enrichment in buffering the effects of severe early neglect on trajectories of psychopathology. Trial Registration ClinicalTrials.gov Identifier: NCT00747396
GOLPH3: a Golgi phosphatidylinositol(4)phosphate effector that directs vesicle trafficking and drives cancer

GOLPH3 is a peripheral membrane protein localized to the Golgi and its vesicles, but its purpose had been unclear. We found that GOLPH3 binds specifically to the phosphoinositide phosphatidylinositol(4)phosphate [PtdIns(4)P], which functions at the Golgi to promote vesicle exit for trafficking to the plasma membrane. PtdIns(4)P is enriched at the trans-Golgi and so recruits GOLPH3. Here, a GOLPH3 complex is formed when it binds to myosin18A (MYO18A), which binds F-actin. This complex generates a pulling force to extract vesicles from the Golgi; interference with this GOLPH3 complex results in dramatically reduced vesicle trafficking. The GOLPH3 complex has been identified as a driver of cancer in humans, likely through multiple mechanisms that activate secretory trafficking. In this review, we summarize the literature that identifies the nature of the GOLPH3 complex and its role in cancer. We also consider the GOLPH3 complex as a hub with the potential to reveal regulation of the Golgi and suggest the possibility of GOLPH3 complex inhibition as a therapeutic approach in cancer.
Supervised Learning for Suicidal Ideation Detection in Online User Content

Early detection and treatment are regarded as the most effective ways to prevent suicidal ideation and potential suicide attempts—two critical risk factors resulting in successful suicides. Online communication channels are becoming a new way for people to express their suicidal tendencies. This paper presents an approach to understand suicidal ideation through online user-generated content with the goal of early detection via supervised learning. Analysing users’ language preferences and topic descriptions reveals rich knowledge that can be used as an early warning system for detecting suicidal tendencies. Suicidal individuals express strong negative feelings, anxiety, and hopelessness. Suicidal thoughts may involve family and friends. And topics they discuss cover both personal and social issues. To detect suicidal ideation, we extract several informative sets of features, including statistical, syntactic, linguistic, word embedding, and topic features, and we compare six classifiers, including four traditional supervised classifiers and two neural network models. An experimental study demonstrates the feasibility and practicability of the approach and provides benchmarks for the suicidal ideation detection on the active online platforms: Reddit SuicideWatch and Twitter.
Overexpression of Fgfr2c causes craniofacial bone hypoplasia and ameliorates craniosynostosis in the Crouzon mouse

ABSTRACT FGFR2c regulates many aspects of craniofacial and skeletal development. Mutations in the FGFR2 gene are causative of multiple forms of syndromic craniosynostosis, including Crouzon syndrome. Paradoxically, mouse studies have shown that the activation (Fgfr2cC342Y; a mouse model for human Crouzon syndrome), as well as the removal (Fgfr2cnull), of the FGFR2c isoform can drive suture abolishment. This study aims to address the downstream effects of pathogenic FGFR2c signalling by studying the effects of Fgfr2c overexpression. Conditional overexpression of Fgfr2c (R26RFgfr2c;βact) results in craniofacial hypoplasia as well as microtia and cleft palate. Contrary to Fgfr2cnull and Fgfr2cC342Y, Fgfr2c overexpression is insufficient to drive onset of craniosynostosis. Examination of the MAPK/ERK pathway in the embryonic sutures of Fgfr2cC342Y and R26RFgfr2c;βact mice reveals that both mutants have increased pERK expression. The contrasting phenotypes between Fgfr2cC342Y and R26RFgfr2c;βact mice prompted us to assess the impact of the Fgfr2c overexpression allele on the Crouzon mouse (Fgfr2cC342Y), in particular its effects on the coronal suture. Our results demonstrate that Fgfr2c overexpression is sufficient to partially rescue craniosynostosis through increased proliferation and reduced osteogenic activity in E18.5 Fgfr2cC342Y embryos. This study demonstrates the intricate balance of FGF signalling required for correct calvarial bone and suture morphogenesis, and that increasing the expression of the wild-type FGFR2c isoform could be a way to prevent or delay craniosynostosis progression. Summary: Increased levels of FGFR2c cause craniofacial bone hypoplasia, microtia and cleft palate, but not craniosynostosis. Introduction of an extra Fgfr2c allele into a mouse model for Crouzon syndrome partially rescues the craniosynostosis phenotype.
Towards a deeper understanding of meaningful use in electronic health records.

OBJECTIVE The purpose of this study was to identify and analyse the US Health Sector concept of the 'Meaningful Use' of electronic health records (EHR's) based on the wide diversity of discourses in the published literature. Meaningful Use is defined as 'use of digital medical and health records to improve quality, safety and efficiency of patient health information'. METHODS Herman Dooyeweerd's philosophical suite of aspects was the chosen research tool used to analyse seminal papers concerning the Meaningful Use of EHRs. The methodology included the identification of the criteria for choosing the papers: application of the criteria to the literature and then selection of the papers and finally the application of Dooyeweerd's aspects to the papers in order to analyse and classify the motivations of the authors. RESULTS As a result of the aspectual analysis of the relevant texts in the seminal papers, two aspects were identified, one as primary and another one as secondary. In addition, the analytic aspect was repeated twice as the primary aspect. Although there were a number of papers distinctively centred on different aspects, overlaps and similarities were also clearly identified. DISCUSSION Dooyeweerd's philosophy is useful in helping to affirm the factors that are important to the authors of the seminal papers and the philosophy helps us to accommodate this diversity in a unique way, therefore enhancing our understanding of approaches in the area. CONCLUSION By employing the lens of Dooyeweerd's aspects, the study enhances the understanding of diversity in one discipline and could potentially provide a new system for the classification of diversity within other related disciplines.
Stable Matching for Dynamic Ride-Sharing Systems

Dynamic ride-sharing systems enable people to share rides and increase the efficiency of urban transportation by connecting riders and drivers on short notice. Automated systems that establish ride-share matches with minimal input from participants provide the most convenience and the most potential for system-wide performance improvement, such as reduction in total vehicle-miles traveled. Indeed, such systems may be designed to match riders and drivers to maximize system performance improvement. However, system-optimal matches may not provide the maximum benefit to each individual participant. In this paper we consider a notion of stability for ride-share matches and present several mathematical programming methods to establish stable or nearly-stable matches, where we note that ride-share matching optimization is performed over time with incomplete information. Our numerical experiments using travel demand data for the metropolitan Atlanta region show that we can significantly increase the stability of ride-share matching solutions at the cost of only a small degradation in system-wide performance.
Dynamics and determinants of the force of infection of dengue virus from 1994 to 2015 in Managua, Nicaragua

Significance A recently licensed dengue vaccine requires pre-vaccination screening for dengue virus (DENV)-specific antibodies and was initially recommended for use only in areas with high DENV transmission. However, DENV transmission intensity is not static. We show that the age at which children acquired DENV-specific immunity more than doubled during the observational period (2004–2015) of a pediatric cohort study in Managua, Nicaragua. A high force of infection of DENV in the 1990s could be explained by the introduction of a novel DENV serotype, while a gradual decline in force of infection could be explained by a long-term population demographic transition. Such dynamics make DENV transmission intensity a problematic metric for designing, implementing, and evaluating interventions and for selecting target age groups/sites. Dengue virus (DENV) is the most prevalent human vector-borne viral disease. The force of infection (FoI), the rate at which susceptible individuals are infected in a population, is an important metric for infectious disease modeling. Understanding how and why the FoI of DENV changes over time is critical for developing immunization and vector control policies. We used age-stratified seroprevalence data from 12 years of the Pediatric Dengue Cohort Study in Nicaragua to estimate the annual FoI of DENV from 1994 to 2015. Seroprevalence data revealed a change in the rate at which children acquire DENV-specific immunity: in 2004, 50% of children age >4 years were seropositive, but by 2015, 50% seropositivity was reached only by age 11 years. We estimated a spike in the FoI in 1997–1998 and 1998–1999 and a gradual decline thereafter, and children age <4 years experienced a lower FoI. Two hypotheses to explain the change in the FoI were tested: (i) a transition from introduction of specific DENV serotypes to their endemic transmission and (ii) a population demographic transition due to declining birth rates and increasing life expectancy. We used mathematical models to simulate these hypotheses. We show that the initial high FoI can be explained by the introduction of DENV-3 in 1994–1998, and that the overall gradual decline in the FoI can be attributed to demographic shifts. Changes in immunity and demographics strongly impacted DENV transmission in Nicaragua. Population-level measures of transmission intensity are dynamic and thus challenging to use to guide vaccine implementation locally and globally.
Iron–gold alloy nanoparticles serve as a cornerstone in hyperthermia-mediated controlled drug release for cancer therapy

Introduction The efficacy of a chemotherapy drug in cancer therapy is highly determined by the ability to control the rate and extent of its release in vivo. However, the lack of techniques to accurately control drug release drastically limits the potency of a chemotherapy drug. Materials and methods Here, we present a novel strategy to precisely monitor drug release under magnetic stimulation. Methotrexate (MTX), an anticancer drug, was covalently functionalized onto iron–gold alloy magnetic nanoparticles (Fe–Au alloy nanoparticles or NFAs) through 2-aminoethanethiol grafting and the ability of this drug–nanoparticle conjugate (NFA–MTX) in limiting HepG2 (liver carcinoma) cell growth was studied. Well-dispersed NFAs were prepared through pyrolysis. Results and discussion Transmission electron microscopy revealed the average nanoparticle size to be 7.22±2.6 nm, while X-ray diffraction showed distinct 2θ peaks for iron and gold, confirming the presence of iron and gold nanoparticles. Inductively coupled plasma mass spectrometry revealed that the amount of NFA–MTX conjugate ingested by HepG2 cancer cells was 1.5 times higher than that ingested by L929 fibroblasts, thereby proving a higher selective ingestion by cancer cells compared to normal cells. Fourier-transform infrared spectroscopy revealed the breakage of Au-S bonds by the heat generated under magnetic field stimulation to release MTX from the NFA–MTX conjugate, triggering a 95% decrease in cellular viability at 100 µg/mL. Conclusion The ability of NFA–MTX to dissociate under the influence of an applied magnetic field provides a new strategy to induce cancer cell death via hyperthermia. Applications in drug delivery, drug development, and cancer research are expected.
The Preliminary Experiences with Three-Dimensional Heads-Up Display Viewing System for Vitreoretinal Surgery under Various Status

ABSTRACT Statement: The current article has not been published elsewhere and has not been submitted simultaneously for publication elsewhere. Purpose: To investigate the preliminary use of three-dimensional (3D) heads-up display (HUD) viewing system for vitreoretinal surgery under various status. Materials and Methods: Nonrandomized case–control study. Consecutive cases to have vitreoretinal surgery under various status were prospectively recruited. Twenty-five-gauge vitrectomy platform and 3D viewing system were used. Main outcomes included: luminous emittance (lux) of endoillumination pipe, surgical duration, the surgeon and residents’ preference and ergonomics. Consecutive patients to have vitreoretinal surgery with the conventional viewing system were recruited as control group following the same inclusion and exclusion criteria and underwent surgeries by the same surgeon with the same microscope and vitrectomy platform. Results: Thirty-one patients (31 eyes; Group Study) and twenty-eight patients (28 eyes; Group Control) were included; without significantly statistical difference in terms of age, gender, main diagnosis, surgical duration, and difficulty rating between both groups (all P > 0.05). Lower endoillumination intensity was needed in Group Study than that in Group Control (10% vs. 35%; 598.7 ± 5.4 vs. 1913.0 ± 12.9 lux, P < 0.001). The surgeon and residents expressed overwhelming preference with the 3D system in both groups. Improved ergonomic was rated in Group Study (4.4 ± 0.8 vs. 3.2 ± 1.0, P < 0.001). Some intraoperative difficulties and discomforts appeared to the surgeon and assistants when using the 3D viewing system. Conclusion: Vitreoretinal surgery under various status can be well finished with the HUD platform by novice at the system. Main benefits included lower endoillumination intensity, enhanced users’ preference, and improved ergonomics. Some further refinements of the system are expected.