Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence of Recipient Site on the Function and Survival of Fat Grafts

Background Autologous fat grafting has become an increasingly common procedure for soft tissue augmentation throughout the body. However, the long-term outcome is always unpredictable because of inconsistent graft survival. Based on the “law of use and disuse,” we speculate that the volume loss of fat grafts will occur when transferred into a site where there is less fat. The purpose of this study is to investigate the cause of high resorption rate from the perspective of fat function after transplantation. Methods Adipose aspirates obtained from routine liposuction were injected into the dorsal site of athymic mice, which have no subcutaneous fat layer. The fat grafts were explanted at days 7, 15, and 30 after transplantation. Changes in fat function were evaluated by measuring the adipocyte size and the expression level of adipose differentiation–related protein. Results After grafting, adipose tissue was replaced by fibrosis, inflammation, and vacuolar tissues gradually over time. The size of fat cells decreased sharply from day 0 to day 7, increased at day 15, and further declined at day 30. Adipose differentiation–related protein expression experienced a dramatic increase at day 7 and then continuously decreased until day 30. Conclusions Assuming that the extrinsic factors influencing fat function and distribution remain stable, capabilities of the redistributed fat to handle free fatty acid and store lipid substance are reduced, leading to substantial tissue atrophy and volume decline after grafting.
Knowledge, interest, and anticipated barriers of pre-exposure prophylaxis uptake and adherence among gay, bisexual, and men who have sex with men who are incarcerated

Criminal justice (CJ) settings disproportionately include populations at high risk for acquiring HIV, and CJ-involved individuals are often at the intersection of multiple overlapping risk factors. However, few studies have examined attitudes about pre-exposure prophylaxis (PrEP) among incarcerated men who have sex with men (MSM). This study explored interest in, knowledge of, and barriers to PrEP uptake among gay, bisexual, and other men who have sex with men at the Rhode Island Department of Corrections. Using semi-structured interviews, 26 MSM were interviewed about PrEP knowledge, interest, timing preferences for provision (e.g. before or after release), and barriers to uptake and adherence during community re-entry. Interviews were coded and analyzed using a general inductive approach. Participants demonstrated low initial knowledge of PrEP but high interest after being told more about it. Participants self-identified risk factors for HIV acquisition, including condomless sex and substance use. In addition, participants preferred provision of PrEP prior to release. Post-release barriers to PrEP uptake and adherence included 1) concerns about costs of PrEP medications; 2) anticipated partner or family disapproval; 3) lack of access to transportation; 4) unstable housing; 5) compounding impacts of multiple hardships leading to a de-prioritization of PrEP and 6) fears of future re-incarceration. These results point to the need for future PrEP interventions among incarcerated populations that address incarceration and PrEP related barriers during community re-entry via wraparound services that address PrEP and incarceration-related barriers.
Coexpression of ETV6/MDS1/EVI1 and ETV6/EVI1 fusion transcripts in acute myeloid leukemia with t(3;12)(q26.2;p13) and thrombocytosis

Katsuya Yamamoto, Kimikazu Yakushijin, Hiroya Ichikawa, Atsuo Okamura, Shigeki Nagao, Seiji Kakiuchi, Keiji Kurata, Shinichiro Kawamoto, Keiji Matsui, Yuji Nakamachi, Jun Saegusa, Hiroshi Matsuoka and Hironobu Minami Division of Medical Oncology/Hematology, Department of Medicine, Kobe University Graduate School of Medicine, Kobe, Japan; Department of Clinical Laboratory, Kobe University Hospital, Kobe, Japan
Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule

Purpose It is well known that zirconia materials have good biocompatibility; however, little is known regarding the mechanism by which cells attach to these materials. The purpose of this study is to elucidate the mechanism of cell attachment. Materials and methods In this study, we examined the surface characteristics of ceria-stabilized zirconia/alumina nanocomposite (NANOZR), yttria-stabilized zirconia (Y-TZP) and commercially pure titanium (CpTi), and we evaluated the initial response of osteoblast-like cells to them with different inhibitors. Results Under the same polishing treatment, the three materials, NANOZR, Y-TZP and CpTi, show similar surface wettability but different surface roughness. Osteoblasts could adhere to the surface of all three materials, and spindle shapes were clearer in serum-containing media compared to PBS and serum-free culture media, suggesting that serum-contained proteins are helpful for the initial cell adhesion and spreading. Cell adhesion and proliferation were disrupted in the presence of EDTA. RGD-peptide interfered with cell proliferation by affecting cell protrusion and stress fibers. Monoclonal antibody against non-RGD type integrin α2β1 enhanced proliferation in Y-TZP, CpTi and culture dish but not in NANOZR. Cell proliferation on NANOZR was specifically inhibited in the presence of heparin. Furthermore, under heparin administration, spindle shape formation was maintained but actin cytoskeleton was disrupted, resulting in loose cellular spreading. Conclusion These results suggest that RGD type integrins and heparin-sensitive protein in coordination regulate cell morphology and proliferation on NANOZR, through the regulation of cell polarity and stress fiber formation, respectively.
Sentence-wise Smooth Regularization for Sequence to Sequence Learning

Maximum-likelihood estimation (MLE) is widely used in sequence to sequence tasks for model training. It uniformly treats the generation/prediction of each target token as multiclass classification, and yields non-smooth prediction probabilities: in a target sequence, some tokens are predicted with small probabilities while other tokens are with large probabilities. According to our empirical study, we find that the non-smoothness of the probabilities results in low quality of generated sequences. In this paper, we propose a sentence-wise regularization method which aims to output smooth prediction probabilities for all the tokens in the target sequence. Our proposed method can automatically adjust the weights and gradients of each token in one sentence to ensure the predictions in a sequence uniformly well. Experiments on three neural machine translation tasks and one text summarization task show that our method outperforms conventional MLE loss on all these tasks and achieves promising BLEU scores on WMT14 English-German and WMT17 Chinese-English translation task.
Peroxisome biogenesis, membrane contact sites, and quality control

Peroxisomes are conserved organelles of eukaryotic cells with important roles in cellular metabolism, human health, redox homeostasis, as well as intracellular metabolite transfer and signaling. We review here the current status of the different co‐existing modes of biogenesis of peroxisomal membrane proteins demonstrating the fascinating adaptability in their targeting and sorting pathways. While earlier studies focused on peroxisomes as autonomous organelles, the necessity of the ER and potentially even mitochondria as sources of peroxisomal membrane proteins and lipids has come to light in recent years. Additionally, the intimate physical juxtaposition of peroxisomes with other organelles has transitioned from being viewed as random encounters to a growing appreciation of the expanding roles of such inter‐organellar membrane contact sites in metabolic and regulatory functions. Peroxisomal quality control mechanisms have also come of age with a variety of mechanisms operating both during biogenesis and in the cellular response to environmental cues.
“My Sister Is the One That Made Me Stay Above Water”: How Social Supports Are Maintained and Strained When Survivors of Intimate Partner Violence Reside in Emergency Shelter Programs

While social support is critical in helping intimate partner violence (IPV) survivors heal and build hope, social isolation is a frequent experience for survivors. For survivors residing in domestic violence (DV) emergency shelters, the isolation from social supports is often exacerbated by shelter rules and policies. This study uses a 20th century feminist framework to understand the ways in which social support networks are maintained and/or strained when survivors reside in DV emergency shelters. Among this sample of 76 survivors, 40 participants identified family members as their support system, with siblings and mothers most frequently identified, and 14 participants identified friends as their social support. Prior to entering the DV shelter, survivors reported that their support networks provided encouragement and motivation along with emotional support, financial and material support, and support with children. Participants spoke about the process of informing their support systems of the transition into a DV shelter, the positive and negative impacts of the transition on their support system, and the roles that frequent telephone calls and texts played in maintaining their support networks. Participants reported positive impacts of residing in a DV shelter including improved emotional and physical health, strengthened emotional relationships, and improved physical and emotional resource provision. For those participants reporting negative experiences, they frequently identified logistical barriers, including the inability to receive visitors at the shelter, the distance between them and their friends and family, and strict rules around curfew and child care. Our findings invite researchers and practitioners to consider further examination of best practices around programming that supports survivors in maintaining social supports while residing in shelter programs
Myeloid‐derived suppressor cells and tumor: Current knowledge and future perspectives

The immunosuppressive features of tumor lesions participate not only as one of the major players inducing cancer progression but also a big challenge for effective immunotherapy. It has been found that immunosuppression associated with chronic inflammatory factors, such as growth factors, cytokines, and chemokines is generated by stroma and tumor cells. Chronic and exhaustive secretion of these mediators triggers the generation of myeloid‐derived suppressor cells (MDSCs) demonstrating one of the key players engaged in tumor immunosuppression. In point of fact, direct cell‐to‐cell contact is a prerequisite for immunosuppressive functions of MDSCs. From the clinical perspective, the frequency of peripheral blood MDSCs is correlated with clinical stage and therapeutic response in various cancers. Furthermore, MDSCs are involved in chemoresistant settings. Altogether, it is a rational therapeutic approach to block the fierce cycle in which MDSCs are developed and infiltrated to favor cancer progression. In this review, we will summarize recent findings of MDSCs in tumor progression and discuss potential therapeutic strategies that could be evaluated in future clinical trials.
Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors

Coculture with adipose-derived stem cells (ADSCs) can stimulate proliferation and migration of melanocytes. To enhance outcomes of skin disorders caused by melanocyte loss or death, mixed transplantation with ADSCs has been suggested. However, role of cocultured ADSCs in proliferation and migration of melanocytes remains unclear. This study determined the effect of ADSCs on production of growth factors and expression levels of intergrins in primary culture of adult human melanocytes with or without ADSCs and in nude mice grafted with such melanocytes. Higher amounts of growth factors for melanocytes, such as bFGF and SCF were produced and released from ADSCs by coculturing with melanocytes. Relative levels of integrins β1, α5, and α6 as well as adhesion to fibronectin and laminin were increased in melanocytes cocultured with ADSCs. Such increases were inhibited by neutralization of bFGF or SCF. Relative levels of bFGF, SCF and integrins were increased in nude mice skin after grafting with melanocyte+ADSC cocultures. Collectively, these results indicate that ADSCs can stimulate proliferation and migration of melanocytes by increasing expression of integrins in melanocytes through upregulation of production/release of melanocyte growth factors such as bFGF and SCF.
Bayesian deep neural networks for low-cost neurophysiological markers of Alzheimer's disease severity

As societies around the world are ageing, the number of Alzheimer's disease (AD) patients is rapidly increasing. To date, no low-cost, non-invasive biomarkers have been established to advance the objectivization of AD diagnosis and progression assessment. Here, we utilize Bayesian neural networks to develop a multivariate predictor for AD severity using a wide range of quantitative EEG (QEEG) markers. The Bayesian treatment of neural networks both automatically controls model complexity and provides a predictive distribution over the target function, giving uncertainty bounds for our regression task. It is therefore well suited to clinical neuroscience, where data sets are typically sparse and practitioners require a precise assessment of the predictive uncertainty. We use data of one of the largest prospective AD EEG trials ever conducted to demonstrate the potential of Bayesian deep learning in this domain, while comparing two distinct Bayesian neural network approaches, i.e., Monte Carlo dropout and Hamiltonian Monte Carlo.
Efficacy and safety of rituximab therapy for membranous nephropathy: a meta-analysis.

OBJECTIVE Membranous nephropathy (MN) is a leading cause of nephrotic syndrome in adults, but the treatment of MN remains controversial. Rituximab, a possible alternative treatment option, represented a new therapeutic hope for the treatment of membranous nephropathy (MN). We performed a meta-analysis to perform the efficacy and safety of rituximab therapy. MATERIALS AND METHODS Either randomized controlled trials (RCTs), cohort studies or case studies were eligible for review and were performed in Medline, Embase, Web of Science, Cochrane database with the computerized searches. The primary outcome measure was remissions and the endpoint outcomes. We assessed the following studies methodological quality independently by using the Cochrane Collaboration tool for assessing risk of bias scale. The primary meta-analyses were performed using fixed or random effects models due to a1n expected clinical diversity. RESULTS Five trials with a total of 351 patients were included. We found significant difference between rituximab and the placebo group on complete remissions rate (OR=1.6, 95%; CI=0.96, 2.66; I2 of 0% indicating no heterogeneity) and eGFR (MD=-0.69, 95% CI=-14.53, 0.73). The factors of remission and no remission MN patients who received treatment with rituximab on remission were proteinuria and albumin, which reported different (MD=7.20, 95% CI=-9.07, -5.33) (MD=10.70, 95% CI=7.23, 14.17). But remains high risk of infusion reactions (OR= 81.37, 95% CI=4.89, 1354.41). No evidence of significant risk of bias was reported. CONCLUSIONS Rituximab had a beneficial effect and remissions of proteinuria on MN during follow-up times, but some adverse events were still unknown. Taking consideration of long-term therapeutic side effects and dose of the drug, we suggest that rituximab might replace cyclophosphamide and steroids as first-line immunosuppressive therapy in MN patients.
Effects of inhibitors of the renin‐angiotensin system on reducing blood pressure and expression of inflammatory factors in CHD patients: A network meta‐analysis

The renin‐angiotensin system (RAS) is an ever‐evolving endocrine system with considerable checks and balances on the production and catabolism of angiotensin peptides most likely due to the manifold effects of angiotensins. We aimed to explore the effects of different inhibitors of RAS on blood pressure and expression of inflammatory factors in patients with coronary heart disease (CHD). We initially searched PubMed, EMBASE and Cochrane Library electronic databases with nine eligible randomized controlled trials enrolled. Direct and indirect evidence was combined to calculate the weighted mean difference value and draw surface under the cumulative ranking curves. The results demonstrated that, compared with placebo and enalapril, ramipril had a better effect on reducing systolic blood pressure after short‐term usage of drugs (<12 months), while perindopril had better effects on reducing diastolic blood pressure and C‐reactive protein expression. Furthermore, after long‐term usage of drugs (≥12 months), there was no significant difference among olmesartan, quinapril and candesartan in the treatment of patients with CHD. Perindopril and ramipril had better effects on inhibiting blood pressure and expression of inflammatory factors among eight inhibitors after short‐term usage of drugs (<12 months); while quinapril had better effects on reducing blood pressure and expression of inflammatory factor after long‐term usage of drugs, and there was little difference in the effects between olmesartan and candesartan (≥12 months). Perindopril may have better short‐term effects on reducing blood pressure and expression of inflammatory factor, while quinapril may have better long‐term effects on reducing blood pressure and expression of inflammatory factor.
Sewer Sediment Bacterial Communities Suggest Potential to Transform Persistent Organic Pollutants

Sediments of combined sewers are seeded with microbes from a variety of sources, and experience varying biogeochemical conditions. A variety of redox processes have been demonstrated to occur within sewer systems, as well as transformation of several recalcitrant xenobiotic contaminants. Illumina sequencing of the 16S ribosomal RNA gene from sediments of three combined sewer systems in the northeastern United States resulted in 10 000 to 47 000 operational taxonomic units per sample. Whereas orders Clostridiales and Bacteroidales, considered human fecal indicators, were dominant in one city's sediments; other cities had communities suggestive of diverse redox processes, including reductive dechlorination of chlorinated organic compounds. Collection systems previously associated with polychlorinated biphenyl (PCB) biotransformation, and those with elevated in situ PCB concentrations, had high abundances of Dehalococcoidetes. The results suggest that wastewater collection systems have intrinsic passive treatment capacity, reducing contaminant loads on water resource recovery facilities and, ultimately, on receiving waters.
Can Social Integration and Social Support Help to Explain Racial Disparities in Health Care Utilization Among Men with Diabetes?

Objective Discovering the mechanisms through which racial and ethnic background influence health is critical to better understanding racial disparities in health among men with a diabetes diagnosis. The present study examines whether social support and social integration mediate or buffer the relationship between race and health care utilization among U.S. men. Method This study used a sub-sample from the 2001 National Health Interview Survey that included Latino, African American, non-Latino White men living with self-reported diabetes (n = 7,148). Results Findings revealed that African American men with high levels of social integration were less likely to forego care (OR = 0.234, [CI = 0.053,1.045]). Also, African American men who did not attend church had higher odds of foregoing care (OR = 0.222, [CI = 0.110,0.448]). Conclusions The effect of social networks operate differently for African American men with diabetes compared to non-Latino white men. Identifying racial differences in the influence of social networks on health will assist in developing interventions that may help to decrease gender and race gaps in health for men with diabetes.
c-Met Signaling Protects from Nonalcoholic Steatohepatitis- (NASH-) Induced Fibrosis in Different Liver Cell Types

Nonalcoholic steatohepatitis (NASH) is the most common chronic, progressive liver disease in Western countries. The significance of cellular interactions of the HGF/c-Met axis in different liver cell subtypes and its relation to the oxidative stress response remains unclear so far. Hence, the present study is aimed at investigating the role of c-Met and the interaction with the oxidative stress response during NASH development in mice and humans. Conditional c-Met knockout (KO) lines (LysCre for Kupffer cells/macrophages, GFAPCre for α-SMA+ and CK19+ cells and MxCre for bone marrow-derived immune cells) were fed chow and either methionine-choline-deficient diet (MCD) for 4 weeks or high-fat diet (HFD) for 24 weeks. Mice lacking c-Met either in Kupffer cells, α-SMA+ and CK19+ cells, or bone marrow-derived immune cells displayed earlier and faster progressing steatohepatitis during dietary treatments. Severe fatty liver degeneration and histomorphological changes were accompanied by an increased infiltration of immune cells and a significant upregulation of inflammatory cytokine expression reflecting an earlier initiation of steatohepatitis development. In addition, animals with a cell-type-specific deletion of c-Met exhibited a strong generation of reactive oxygen species (ROS) by dihydroethidium (hydroethidine) (DHE) staining showing a significant increase in the oxidative stress response especially in LysCre/c-Metmut and MxCre/c-Metmut animals. All these changes finally lead to earlier and stronger fibrosis progression with strong accumulation of collagen within liver tissue of mice deficient for c-Met in different liver cell types. The HGF/c-Met signaling pathway prevents from steatosis development and has a protective function in the progression to steatohepatitis and fibrosis. It conveys an antifibrotic role independent on which cell type c-Met is missing (Kupffer cells/macrophages, α-SMA+ and CK19+ cells, or bone marrow-derived immune cells). These results highlight a global protective capacity of c-Met in NASH development and progression.