Miracle-dz/UniFAR-FTUs huggingface dataset:
A Tale of Two Studies: Equal Opportunity Climate and Mission Readiness

Abstract : Workplace discrimination and strain are both linked to mission readiness, as they yield decreased job performance (Cropanzano, Rupp, and Byrne, 2003; Goldman, Gutek, Stein, and Lewis, 2006). Together, they cost US organizations over a billion dollars per day (American Institute of Stress, 2012; Center for American Progress, 2012). Surprisingly, despite these high costs and associations with important organizational factors, little is known about how the two constructs are related. Whereas research suggests that individuals who directly experience harassment are prone to some forms of strain (Goldman et al., 2006), there is a paucity of empirical investigations examining the impact of workplace discrimination on burnout among individuals who simply work in environments where harassment is present. We conducted two studies investigating aspects of equal opportunity climate as predictors of emotional exhaustion among Department of Defense personnel. The first study focused on the leader in terms of task-oriented leadership and leader equal opportunity behaviors and on the unit in terms of unit cohesion. The results of this study indicated that being a good leader in terms of simply focusing on mission accomplishment is insufficient to prevent emotional exhaustion. The second study presents a conditional process model that describes a psychological process in which equal opportunity climate leads to emotional exhaustion indirectly through perceptions of organizational support. Hence, it (1) describes how an equal opportunity climate impacts emotional exhaustion, and (2) identifies personality traits that determine for whom this process is most important. Both studies suggest opportunities for the development of leaders in the Department of Defense.
Hepato-Incretin Function of GLP-1: Novel Concept and Target in Type 1 Diabetes

The liver is a key player in glucose regulation, and several factors are involved in this regulation (1). Glucagon, autonomic nerves, and the adrenals stimulate hepatic glucose production to allow sufficient brain glucose delivery. Insulin inhibits hepatic glucose production, preventing excess hepatic glucose release. This is of particular importance after meals, as is evident from the negative correlation between early insulin response after meals and postprandial glucose (2). Therefore, low insulin and high glucagon levels—such as observed in type 1 and type 2 diabetes—sustain liver glucose production, thereby resulting in postprandial and fasting hyperglycemia. Thus, reducing hepatic glucose production is an important target in the treatment of diabetes (3). The incretin hormone GLP-1 inhibits hepatic glucose production through its ability to stimulate insulin secretion and inhibit glucagon secretion (4). As GLP-1 is released after meal ingestion in proportion to meal size (5), this effect allows meal-dependent inhibition of hepatic glucose production. However, GLP-1 may also inhibit endogenous glucose production through an islet-independent mechanism (6–9). This allows not only a rapid shutoff of endogenous glucose production after meal ingestion but also incretin therapy to be potent in insulin-deficient diabetes. A study in the current issue of Diabetes presents convincing evidence for such an islet-independent inhibition of endogenous glucose production by GLP-1 (10). The study by Jun et al. (10) leveraged the unique glucagon receptor …
Current concepts in the treatment of radial head fractures in the adult. A clinical and biomechanical approach.

The treatment of simple radial head fractures type Mason-Hotchkiss 1 and 2 is unequivocal. In the case of a displacement of less than 2 mm (Mason-Hotchkiss 1), functional conservative treatment is indicated. If the displacement is more than 2 mm (Mason-Hotchkiss 2) internal fixation is indicated, combined with treatment of the soft-tissue injuries. The treatment of comminuted radial head fractures Mason-Hotchkiss type 3 is more controversial, as they are usually associated with ligament injuries. Conservative treatment appears to have unsatisfactory results. Opinions differ on surgical treatment, i.e. 1) excision of the radial head; 2) reconstruction by means of internal fixation; 3) excision and reconstruction with a prosthesis. Basic experimental research on human cadaver elbows shows the importance of the conservation of the radial head, especially in case of associated soft tissue injuries. In most cases a stable osteosynthesis is impossible, and in case of associated ligament disruptions, resection and reconstruction by means of a rigid radial head prosthesis should be considered. Experimental research demonstrates promising results for rigid radial head prostheses in human cadaver elbows with a resected radial head and failing medical collateral ligament. Short-term clinical studies seem to confirm this, but long-term results are as yet unknown.
PDZD8 Is a Novel Gag-Interacting Factor That Promotes Retroviral Infection

ABSTRACT In a yeast two-hybrid screen for cellular factors that could interact with human immunodeficiency virus type 1 (HIV-1) Gag protein, we identified PDZD8 and confirmed the interaction by coimmunoprecipitation (co-IP). PDZD8 overexpression promoted the initiation of reverse transcription and increased infection by pseudotyped retroviruses independent of the route of viral entry, while transient knockdown of endogenous levels decreased HIV-1 infection. A mutant of PDZD8 lacking a predicted coiled-coil domain in its Gag-interacting region failed to bind Gag and promote HIV-1 infection, identifying the domain of PDZD8 required for mediating these effects. As such, we identify PDZD8 as a novel positive mediator of retroviral infection.
Regulation of tradeoffs between plant defenses against pathogens with different lifestyles

Plants activate distinct defense responses depending on the lifestyle of the attacker encountered. In these responses, salicylic acid (SA) and jasmonic acid (JA) play important signaling roles. SA induces defense against biotrophic pathogens that feed and reproduce on live host cells, whereas JA activates defense against necrotrophic pathogens that kill host cells for nutrition and reproduction. Cross-talk between these defense signaling pathways has been shown to optimize the response against a single attacker. However, its role in defense against multiple pathogens with distinct lifestyles is unknown. Here we show that infection with biotrophic Pseudomonas syringae, which induces SA-mediated defense, rendered plants more susceptible to the necrotrophic pathogen Alternaria brassicicola by suppression of the JA signaling pathway. This process was partly dependent on the cross-talk modulator NPR1. Surprisingly, this tradeoff was restricted to tissues adjacent to the site of initial infection; A. brassicicola infection in systemic tissue was not affected. Even more surprisingly, tradeoff occurred only with the virulent Pseudomonas strain. Avirulent strains that induced programmed cell death (PCD), an effective plant-resistance mechanism against biotrophs, did not cause suppression of JA-dependent defense. This result might be advantageous to the plant by preventing necrotrophic pathogen growth in tissues undergoing PCD. Our findings show that plants tightly control cross-talk between SA- and JA-dependent defenses in a previously unrecognized spatial and pathogen type-specific fashion. This process allows them to prevent unfavorable signal interactions and maximize their ability to concomitantly fend off multiple pathogens.
Reconstitution of DNA topoisomerase VI of the thermophilic archaeon Sulfolobus shibatae from subunits separately overexpressed in Escherichia coli.

DNA topoisomerase VI from the hyperthermophilic archaeon Sulfolobus shibatae is the prototype of a novel family of type II DNA topoisomerases that share little sequence similarity with other type II enzymes, including bacterial and eukaryal type II DNA topoisomerases and archaeal DNA gyrases. DNA topoisomerase VI relaxes both negatively and positively supercoiled DNA in the presence of ATP and has no DNA supercoiling activity. The native enzyme is a heterotetramer composed of two subunits, A and B, with apparent molecular masses of 47 and 60 kDa, respectively. Here wereport the overexpression in Escherichia coli and the purification of each subunit. The A subunit exhibits clusters of arginines encoded by rare codons in E.coli . The expression of this protein thus requires the co-expression of the minor E.coli arginyl tRNA which reads AGG and AGA codons. The A subunit expressed in E.coli was obtained from inclusion bodies after denaturation and renaturation. The B subunit was overexpressed in E.coli and purified in soluble form. When purified B subunit was added to the renatured A subunit, ATP-dependent relaxation and decatenation activities of the hyperthermophilic DNA topoisomerase were reconstituted. The reconstituted recombinant enzyme exhibits a specific activity similar to the enzyme purified from S.shibatae . It catalyzes transient double-strand cleavage of DNA and becomes covalently attached to the ends of the cleaved DNA. This cleavage is detected only in the presence of both subunits and in the presence of ATP or its non-hydrolyzable analog AMPPNP.
Design of a high-voltage driver based on low-voltage CMOS with an adapted level shifter optimized for a wide range of supply voltage

This paper presents the design of a high-voltage driver with an adapted level shifter for switching converters. The proposed HV-driver and level shifter are based on stacked standard CMOS, therefore the design is technology independent. The circuit is designed in 65-nm TSMC technology with a nominal voltage of 2.5 V and optimized for arbitrary supply voltages from 2.6 V to 6.0 V. This range is extended by 41.7% when compared against common drivers and level shifters with the circuit being suitable for a supply voltage range of 2.4 V between 2.6 V and 5.0 V. The total area of the designed level shifter is about 3.8% of that required for similar circuit in previous work.
Moderate prenatal alcohol exposure and serotonin genotype interact to alter CNS serotonin function in rhesus monkey offspring.

BACKGROUND Moderate prenatal alcohol exposure can contribute to neurodevelopmental impairments and disrupt several neurotransmitter systems. We examined the timing of moderate level alcohol exposure, serotonin transporter gene polymorphic region variation (rh5-HTTLPR), and levels of primary serotonin and dopamine (DA) metabolites in cerebrospinal fluid (CSF) in rhesus monkeys. METHODS Thirty-two 30-month old rhesus monkeys (Macaca mulatta) from 4 groups of females were assessed: (i) early alcohol-exposed group (n = 9), in which mothers voluntarily consumed 0.6 g/kg/d alcohol solution on gestational days 0 to 50; (ii) middle-to-late gestation alcohol-exposed group (n = 6), mothers consumed 0.6 g/kg/d alcohol solution on gestational days 50 to 135; (iii) a continuous-exposure group (n = 8), mothers consumed 0.6 g/kg/d alcohol solution on gestational days 0 to 135; and (iv) controls (n = 9), mothers consumed an isocaloric control solution on gestational days 0 to 50, 50 to 135, or 0 to 135. Serotonin transporter promoter region allelic variants (homozygous s/s or heterozygous s/l vs. homozygous l/l) were determined. We examined CSF concentrations of the 5-HT and DA metabolites, 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA), respectively, at baseline and 50 hours after separation from cage-mates, when the monkeys were 30 months old. RESULTS Early- and middle-to-late gestation-alcohol exposed monkeys carrying the short allele had lower concentrations of 5-HIAA in CSF relative to other groups. Concentrations of 5-HIAA in CSF were lower for s allele carriers and increased from baseline relative to pre-separation values, whereas 5-HIAA levels in l/l allele carriers were not affected by separation. Monkeys carrying the short allele had lower basal concentrations of HVA in CSF compared with monkeys homozygous for the long allele. CONCLUSION Carrying the s allele of the 5-HT transporter increased the probability of reduced 5-HIAA in early- and middle-to-late gestation alcohol-exposed monkeys and reduced HVA at baseline. These findings that prenatal alcohol exposure altered central 5-HT activity in genetically sensitive monkeys raise questions about whether abnormal serotonin biological pathways could underlie some of the psychiatric disorders reported in fetal alcohol spectrum disorder.
Clinical Features and Treatments of Syphilitic Uveitis: A Systematic Review and Meta-Analysis

Purpose To investigate the clinical features and efficacies of treatments for syphilitic uveitis. Methods PubMed was searched for studies of syphilitic uveitis published between January 1990 and October 2016. The clinical features were summarized and appraised. The pooled success rate was defined as an improved or maintained final visual acuity and was calculated with 95% confidence intervals (CIs). Heterogeneity, subgroup analysis, sensitivity analysis, and publication bias were assessed. Results Thirty-two studies involving 670 patients were analyzed. The most common type of syphilitic uveitis was papillitis. The pooled success rate was 0.91 (95% CI 0.84–0.97) for antibacterial agents alone (15 studies, 286 patients); 0.95 (95% CI 0.91–0.98) for antibacterial agents and systemic corticosteroids combined (11 studies, 245 patients); and 0.91 (95% CI 0.80–0.98) for antibacterial agents, systemic corticosteroids, and other immunosuppressants combined (3 studies, 73 patients). Subgroup analyses revealed no correlations of the efficacy of antibacterial agent monotherapy with study characteristics, such as human immunodeficiency virus coinfection status. Conclusions This systematic review and meta-analysis revealed the efficacy of antibacterial agents for treating syphilitic uveitis. Coadministration of systemic corticosteroids or immunosuppressants did not elicit further improvements in the clinical outcomes of antibacterial agents.
Liver inflammation and cytokine production, but not acute phase protein synthesis, accompany the adult liver progenitor (oval) cell response to chronic liver injury

Oval cells are facultative liver progenitor cells, which are invoked during chronic liver injury in order to replenish damaged hepatocytes and bile duct cells. Previous studies have observed inflammation and cytokine production in the liver during chronic injury. Further, it has been proposed that inflammatory growth factors may mediate the proliferation of oval cells during disease progression. We have undertaken a detailed examination of inflammation and cytokine production during a time course of liver injury and repair, invoked by feeding mice a choline‐deficient, ethionine‐supplemented (CDE) diet. We show that immediately following initial liver injury, B220‐expressing leucocytes transiently infiltrate the liver. This inflammatory response occurred immediately before oval cell numbers began to expand in the liver, suggesting that the two events may be linked. Two waves of liver cytokine production were observed during the CDE time course. The first occurred shortly following commencement of the diet, suggesting that it may represent a hepatic acute phase response. However, examination of acute phase marker expression in CDE‐fed mice did not support this hypothesis. The second wave of cytokine expression correlated with the expansion of oval cell numbers in the liver, suggesting that these factors may mediate oval cell proliferation. No inflammatory signalling was detected following withdrawal of the injury stimulus. In summary, our results document a close correlation between inflammation, cytokine production and the expansion of oval cells in the liver during experimental chronic injury.
Variability of Germinative Potential among Pathogenic Species of Aspergillus

ABSTRACT The objective of our study was to evaluate parameters influencing the germination of Aspergillus conidia. Inoculum concentration and age significantly influenced germination. Different incubation temperatures revealed significant differences among Aspergillus species. The internal human milieu provides the ideal conditions for the development of invasive disease by Aspergillus fumigatus but restricts invasion by Aspergillus flavus and Aspergillus niger.
Identification of uniparental disomy in phenotypically abnormal carriers of isochromosomes or Robertsonian translocations.

Carriers of either homologous or non-homologous acrocentric rearrangements are at an increased risk for aneuploidy, and, thus, for uniparental disomy (UPD). Abnormal phenotypes due to genomic imprinting are associated with UPD for the acrocentric chromosomes 14 and 15. The purpose of this study was to determine the prevalence of UPD in a population with acrocentric rearrangements (either an isochromosome or a Robertsonian translocation) and abnormal phenotypes. Fifty individuals were studied. Of the 50 rearrangements, two were homologous rearrangements and both showed UPD. Forty-eight were non-homologous Robertsonian translocations, of which two showed UPD. This study demonstrates that UPD explains the abnormal phenotypes in some balanced carriers of acrocentric rearrangements. Our results and the large number of case reports in the literature suggest that patients with abnormal phenotypes and acrocentric rearrangements of chromosomes 14 or 15 should be tested for UPD.
High‐resolution localization of hyaluronic acid in the golden hamster oocyte‐cumulus complex by use of a hyaluronidase‐gold complex

The distribution of hyaluronic acid in the oocyte‐cumulus complexes collected from the oviduct ampulla of superovulated hamsters was revealed by use of hyaluronidase coupled to colloidal gold. On thin sections of Lowicryl‐embedded oocyte‐cumulus complexes, gold particles were associated specifically with interconnecting fibrillar materials that make up the cumulus matrix. Inside the cumulus cells, gold particles were found over the cisternal membrane of the rough endoplasmic reticulum, in the contents of lysosomes and multivesicular bodies, and over Golgi vesicles of some cumulus cells. A high concentration of gold labeling was observed over the peripheral condensed chromatin and perinucleolar components in the nucleus. The cell surface of the cumulus cells also appeared to be labeled. Gold particles, however, were absent over the mitochondria and lipid vacuoles. In the oocytes, labeling was found to be associated mainly with rough endoplasmic reticulum and arrays of lamellar structures; cortical granules, mitochondria, and coated vesicles were essentially devoid of gold particles. Gold particles were also seen along the plasma membrane of the oocytes and within the perivitelline space. The zona pellucida was not labeled by hyaluronidase‐gold. Different control experiments confirmed the specificity of the labeling. Digestion of thin sections with hyaluronidase prior to incubation with hyaluronidase‐gold abolished the initial reaction, whereas treatment of thin sections with chondroitinase did not prevent labeling of oocyte‐cumulus complexes by hyaluronidase‐gold. Although the function of hyaluronic acid in the oocyte‐cumulus complex at the time of ovulation and fertilization is not known, the high concentration of this particular compound in the cumulus matrix and the cumulus cells and its specific locations in the perivitelline space and in the superovulated oocytes implicate the significance of its presence and warrant future investigations.
Molecular basis of congenital insensitivity to pain with anhidrosis (CIPA): Mutations and polymorphisms in TRKA (NTRK1) gene encoding the receptor tyrosine kinase for nerve growth factor

Congenital insensitivity to pain with anhidrosis (CIPA), also referred to as hereditary sensory and autonomic neuropathy type IV (HSAN‐IV), is an autosomal recessive hereditary disorder characterized by recurrent episodic fever, anhidrosis (inability to sweat), absence of reaction to noxious stimuli, self‐mutilating behavior, and mental retardation. The TRKA (NTRK1) gene located on chromosome 1 (1q21‐q22), consists of 17 exons and spans at least 23 kb. TRKA encodes the receptor tyrosine kinase (RTK) for nerve growth factor (NGF) and is the gene responsible for CIPA. Defects in NGF signal transduction at the TRKA receptor lead to failure to support survival of sympathetic ganglion neurons and nociceptive sensory neurons derived from the neural crest. Thirty‐seven different TRKA mutations, identified in patients in various countries, including nine frameshift, seven nonsense, seven splice, and 14 missense mutations, are distributed in an extracellular domain involved in NGF binding, as well as in the intracellular signal‐transduction domain. Extensive analysis of CIPA mutations and associated intragenic polymorphisms should facilitate detection of CIPA mutations and aid in the diagnosis and genetic counseling of this painless but severe genetic disorder with devastating complications. In addition, naturally occurring TRKA missense mutations with loss of function provide considerable insight into the structure–function relationship in the RTK family. Further, molecular pathology of CIPA would provide unique opportunities to explore critical roles of the autonomic sympathetic nervous system as well as peripheral sensory nervous system that transmit noxious stimuli in humans. Hum Mutat 18:462–471, 2001. © 2001 Wiley‐Liss, Inc.
Tolerability and Usefulness of Iohexol in Cardioangiography

The first investigation of iohexol in human cardioangiography is presented. Slight subjective reactions as well as moderate and transient influence on hemodynamics and ECG parameters were recorded. Analysis of serum enzymes indicated no myocardial injury. Good or excellent films of the left ventricle, aortic root and coronary arteries were obtained.
The International Headache Society (IHS) Headache Classification as Applied to a Headache Clinic Population

Four hundred patients attending a headache clinic were classified using the IHS criteria. The majority required more than two, often three or four, diagnoses. Even though migraine was the most common diagnosis, only 1/4 of those with a migraine diagnosis had it as the only diagnosis. Seventy-five percent of migraine patients had coexistent chronic tension-type headache (CTTH), drug-induced headache or both. Ninety-six percent of patients diagnosed as having migraine with aura also suffered from migraine without aura. More than 1/3 of patients (37.7%) attending the clinic suffered from chronic daily headache (CDH) (chronic cluster headache excluded), which is not included as a separate entity in the IHS classification. Pure CTTH formed only a small minority of CDH, whereas 86.6% of CDH had migraine as one of the diagnoses. Drug-induced headache was a prominent second or third diagnosis. The advantages and disadvantages of multiple verses single diagnosis in CDH and the need to recognize the natural history of headache disorders in the classification are discussed.