Miracle-dz/UniFAR-FTUs huggingface dataset:
Rsp5 is required for the nuclear export of mRNA of HSF1 and MSN2/4 under stress conditions in Saccharomyces cerevisiae

Rsp5 is an essential and multi‐functional E3 ubiquitin ligase in Saccharomyces cerevisiae. We previously isolated the Ala401Glu rsp5 mutant that is hypersensitive to various stresses. In rsp5A401E cells, the transcription of the stress protein genes was defective. To understand the mechanism by which Rsp5 regulates the expression of stress proteins, we analyzed the expression and localization of two major transcription factors, Hsf1 and Msn2/4, required for stress protein gene expression in S. cerevisiae. The mRNA levels of HSF1 and MSN2/4 in rsp5A401E cells were slightly lower than those of wild‐type cells. An interesting finding is that the protein levels of HSF1 and Msn2/4 were remarkably defective in rsp5A401E cells after exposure to temperature up‐shift and ethanol, although these proteins are mainly localized in the nucleus under these stress conditions. We also showed that the mRNAs of HSF1 and MSN2/4 were accumulated in the nucleus of rsp5A401E cells after exposure to temperature up‐shift and ethanol, and even under non‐stress conditions, suggesting that Rsp5 is required for the nuclear export of these mRNAs. These results indicate that, in response to environmental stresses, Rsp5 primarily regulates the expression of Hsf1 and Msn2/4 at the post‐transcriptional level and is involved in the repair system of stress‐induced abnormal proteins.
Urodynamic outcome after surgery for severe prolapse and potential stress incontinence.

OBJECTIVE Women with severe prolapse may be paradoxically continent because of kinking of the urethra. It is currently a common practice to perform urethropexy in women who demonstrate stress incontinence on preoperative reduction of the prolapse with a pessary. We compared the urodynamic outcomes after reconstructive operations that included suspending urethropexy with outcomes after those that did not. STUDY DESIGN A review was performed of the charts of the Gynecologic Urology Clinic at Los Angeles County-University of Southern California Women's and Children's Hospital from 1991-1997 of patients with grade III uterovaginal prolapse or procidentia in whom the pessary test was used to determine whether urethropexy was included in the reconstructive operation. Urodynamic outcomes were compared statistically with the Fisher exact test, and P < or =. 05 denoted statistical significance. RESULTS Fifty-five patients underwent urethropexy in addition to repair of the prolapse, and 70 underwent reconstruction alone. Twenty-three patients in the first group and 20 in the second were available for a mean urodynamic follow-up of 3.5 years. In the urethropexy group 7 (30%) patients had de novo detrusor instability and 1 (4%) had stress incontinence. In the reconstruction-only group 1 (5%) patient had detrusor instability and none had stress incontinence. CONCLUSIONS Preoperative barrier testing is useful in identifying patients who do not require an antiincontinence procedure. Prophylactic Burch retropubic urethropexy increases the incidence of bladder instability.
Adult Living Liver Donors have Excellent Long‐Term Medical Outcomes: The University of Toronto Liver Transplant Experience

Right lobe living donor liver transplantation is an effective treatment for selected individuals with end‐stage liver disease. Although 1 year donor morbidity and mortality have been reported, little is known about outcomes beyond 1 year. Our objective was to analyze the outcomes of the first 202 consecutive donors performed at our center with a minimum follow‐up of 12 months (range 12–96 months). All physical complications were prospectively recorded and categorized according to the modified Clavien classification system. Donors were seen by a dedicated family physician at 2 weeks, 1, 3 and 12 months postoperatively and yearly thereafter. The cohort included 108 males and 94 females (mean age 37.3 ± 11.5 years). Donor survival was 100%. A total of 39.6% of donors experienced a medical complication during the first year after surgery (21 Grade 1, 27 Grade 2, 32 Grade 3). After 1 year, three donors experienced a medical complication (1 Grade 1, 1 Grade 2, 1 Grade 3). All donors returned to predonation employment or studies although four donors (2%) experienced a psychiatric complication. This prospective study suggests that living liver donation can be performed safely without any serious late medical complications and suggests that long‐term follow‐up may contribute to favorable donor outcomes.
Impact of Pulmonary Vein Cryoballoon Ablation on Bronchial Injury

There is a paucity of data on the mechanisms of cough and hemoptysis that sometimes ensue from cryoballoon ablation of pulmonary veins (Cryo‐PV). This study specifically examined the impact of ultra‐cold (≤−60 °C, 3 minutes), prolonged (>−55 °C, 6 minutes), and conventional (>−55 °C, 3 minutes) Cryo‐PV on lung/bronchial injury.
GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage1[W][OPEN]

An Arabidopsis gene product affects the size of the pectic polysaccharide, rhamnogalacturonan I, in seed coat mucilage. The function of a putative galacturonosyltransferase from Arabidopsis (Arabidopsis thaliana; At1g02720; GALACTURONOSYLTRANSFERASE-LIKE5 [AtGATL5]) was studied using a combination of molecular genetic, chemical, and immunological approaches. AtGATL5 is expressed in all plant tissues, with highest expression levels in siliques 7 DPA. Furthermore, its expression is positively regulated by several transcription factors that are known to regulate seed coat mucilage production. AtGATL5 is localized in both endoplasmic reticulum and Golgi, in comparison with marker proteins resident to these subcellular compartments. A transfer DNA insertion in the AtGATL5 gene generates seed coat epidermal cell defects both in mucilage synthesis and cell adhesion. Transformation of atgatl5-1 mutants with the wild-type AtGATL5 gene results in the complementation of all morphological phenotypes. Compositional analyses of the mucilage isolated from the atgatl5-1 mutant demonstrated that galacturonic acid and rhamnose contents are decreased significantly in atgatl5-1 compared with wild-type mucilage. No changes in structure were observed between soluble mucilage isolated from wild-type and mutant seeds, except that the molecular weight of the mutant mucilage increased 63% compared with that of the wild type. These data provide evidence that AtGATL5 might function in the regulation of the final size of the mucilage rhamnogalacturonan I.
Exchange Rates, Foreign Currency Exposure and Sovereign Risk

We quantify the causal link between exchange rate movements and sovereign risk of 16 major emerging market economies (EMEs) by means of structural vector autoregressive models (SVARs) using data from 10/2004 through 12/2016. We apply a novel data based identification approach of the structural shocks that allows to account for the complex interrelations within the triad of exchange rates, sovereign risks and interest rates. We find that the direction and size of the response of sovereign risk to FX rate movements depend on the type of exchange rate measure we look at and on the size of the net foreign currency exposure of an economy. A depreciation of the domestic currency against the USD increases sovereign risk. In contrast, a depreciation of the effective exchange rate turns out to have only a significant effect on sovereign risk for countries with large negative net foreign currency exposures of the private sector. In this case, a depreciation of the NEER also induces an increase in sovereign risk. We conclude that the `financial channel' is more important in the transmission of exchange rate shocks to sovereign risk in comparison with the traditional `net trade channel'.
Characterizing monoclonal antibody structure by carboxyl group footprinting

Structural characterization of proteins and their antigen complexes is essential to the development of new biologic-based medicines. Amino acid-specific covalent labeling (CL) is well suited to probe such structures, especially for cases that are difficult to examine by alternative means due to size, complexity, or instability. We present here a detailed account of carboxyl group labeling (with glycine ethyl ester (GEE) tagging) applied to a glycosylated monoclonal antibody therapeutic (mAb). The experiments were optimized to preserve the structural integrity of the mAb, and experimental conditions were varied and replicated to establish the reproducibility of the technique. Homology-based models were generated and used to compare the solvent accessibility of the labeled residues, which include aspartic acid (D), glutamic acid (E), and the C-terminus (i.e., the target probes), with the experimental data in order to understand the accuracy of the approach. Data from the mAb were compared to reactivity measures of several model peptides to explain observed variations in reactivity. Attenuation of reactivity in otherwise solvent accessible probes is documented as arising from the effects of positive charge or bond formation between adjacent amine and carboxyl groups, the latter accompanied by observed water loss. A comparison of results with previously published data by Deperalta et al using hydroxyl radical footprinting showed that 55% (32/58) of target residues were GEE labeled in this study whereas the previous study reported 21% of the targets were labeled. Although the number of target residues in GEE labeling is fewer, the two approaches provide complementary information. The results highlight advantages of this approach, such as the ease of use at the bench top, the linearity of the dose response plots at high levels of labeling, reproducibility of replicate experiments (<2% variation in modification extent), the similar reactivity of the three target probes, and significant correlation of reactivity and solvent accessible surface area.
Achieving Regional Convergence through the Role of Foreign Direct Investment and Portfolio Investment: Evidence from ASEAN+3

T he purpose of this study is to analyze the effect of foreign direct investment and portfolio investment on the convergence occurrence of economic growth of countries in the ASEAN + 3 region and to determine the time or speed required to achieve convergence. The type of data used in this research is secondary data panel which is combination between time series data and cross-section data with annual time period 2001-2015 and number of ASEAN member country that is Brunei Darussalam, Cambodia, Indonesia, Lao PDR, Malaysia, Myanmar, Philippines, Singapore, Thailand, Viet Nam and plus three countries namely China, Korea and Japan. So the total observation is 195. The analytical method used is the Arellano Bond dynamic panel. The results of the study show that the first lag variable of economic growth, Foreign Direct Investment (FDI) and portfolio investment have a significant influence on economic growth in ASEAN + 3, but not on ASEAN without China, Japan, and Republic Korea. Foreign Direct Investment (FDI) has a greater influence on changes in economic growth than portfolio investment. Half-life conditional convergence shows a value of 10.63 years which means the time required achieving steady-state conditions of the convergence process or the time required to achieve half of the convergence at convergence rates reaches 0.065% / year. Meanwhile, the influence of portfolio investment provides a greater speed of adjustment to the convergence process than foreign direct investment, but in a half-life of convergence, foreign direct investment is faster than the influence of portfolio investment.
Knowledge Transfer from Multinationals through Labour Mobility: Are There Effects on Productivity, Product Sophistication and Exporting?

ABSTRACT We investigate whether labour mobility from foreign-owned firms to local firms in the host economy is associated with an increase in productivity, export product complexity and other export indicators of domestic firms. Based on employer-employee level data from Estonia, we confirm that hiring employees with experience from foreign-owned firms is associated with an increase in the total factor productivity (TFP) of the firm and the higher export propensity and breadth of export markets or products. However, on average, these within-firm effects on TFP appear to be not working through increases in the level of the complexity of the export product portfolio of domestically owned firms. One implication of this result is that other channels of upgrading than changes in the Hausmann-Hidalgo export product complexity measure may be more important in this context; for example, such as upgrading the tasks or activities in the global value chain of a product.
Controllability of Multiagent Networks With Antagonistic Interactions

This paper addresses the controllability of a class of antagonistic multiagent networks with both positive and negative edges. All the agents of the multiagent network run a consensus algorithm using a signed Laplacian. Based on the generalized equitable partition, we propose a graph-theoretic characterization of an upper bound on the controllable subspace. Then, we provide a necessary condition for the controllability of the system and give an algorithm to compute the partition. Furthermore, we prove that for a structurally balanced network, the controllability is equivalent to that of the corresponding all-positive network, if the leaders are chosen from the same vertex set. Several examples are given to illustrate these results.
Haemangiosarcoma of the interventricular septum in a dog

Abstract CASE HISTORY: A 5-year-old male neutered Labrador Retriever Afghan crossbred dog was examined after collapsing. The dog was recumbent, dyspnoeic and mildly tachypnoeic. There was a tachyarrhythmia (300 beats per minute) and subcutaneous oedema of the ventral neck and right forelimb. CLINICAL FINDINGS: The dog had a sustained ventricular tachycardia originating in the right ventricle and on echocardiography a mass was identified in the interventricular septum. Due to the poor prognosis the owners agreed to euthanasia of the dog. PATHOLOGICAL FINDINGS: A reddish-grey mass was found in the interventricular septum and smaller red foci found scattered throughout the myocardium. There was a single, raised, splenic nodule with several smaller red foci within the splenic parenchyma. Hepatic congestion, pancreatic oedema, ascites and subcutaneous oedema of the right forelimb and neck were present. Sections of the splenic nodule, interventricular septal mass and both ventricular-free walls showed neoplastic mesenchymal cells. DIAGNOSIS: Haemangiosarcoma of the myocardium and spleen with right-sided congestive heart failure. CLINICAL RELEVANCE: This case report describes an atypical location for haemangiosarcoma. The investigation supports the use of echocardiography as a component of the protocol for staging haemangiosarcoma even in the absence of apericardial effusion. It also provides further evidence for the inclusion of intracardiac neoplasia as a differential diagnosis for dogs with unexplained cardiac arrhythmias.
Comparative assessment of immunization coverage of migrant children between national immunization program vaccines and non-national immunization program vaccines in East China

This study aimed to describe the disparities in immunization coverage between National Immunization Program (NIP) vaccines and non-NIP vaccines in Yiwu and to identify potential determinants. A face-to-face interview-based questionnaire survey among 423 migrant children born from 1 June 2010 to 31 May 2013 was conducted. Immunization coverage was estimated according to the vaccines scheduled at different age, the birth cohorts, and socio- demographic characteristics. Single-level logistic regression analysis was applied to identify the determinants of coverage of non-NIP vaccines. We found that NIP vaccines recorded higher immunization coverage compared with non-NIP vaccines (87.9100%– vs 0%-74.8%). Among the non-NIP vaccines, varicella vaccine (VarV) recorded the highest coverage of 85.4%, which was introduced in 1998; while 7-valent pneumococcal conjugate vaccine(PCV7) recorded the lowest coverage of 0% for primary series, which was introduced recently. Lower coverage rate of non-NIP vaccines was significantly associated with more siblings in household, shorter duration of living in the surveyed areas, lower family income, mother with a job, mother with poor awareness of vaccination, and mother with lower education level. We found the immunization coverage rate of non-NIP vaccines was significant lower than that of NIP vaccines. Expansion of NIP to include non-NIP vaccines can provide better protection against the vaccine preventable diseases through increased immunization coverage.
The Bo1-specific PCR marker AWW5L7 is predictive of boron tolerance status in a range of exotic durum and bread wheats.

High soil boron (B) constitutes a major soil problem in many parts of the world, particularly in low-rainfall areas and land under irrigation. Low accumulation of B in the shoot or grain of cereal crops is correlated with the maintenance of biomass production and grain yield under high B conditions, suggesting that this trait is an important component of field tolerance. A novel screening protocol to measure B accumulation in aerated and supported hydroponics was validated using a set of known and exotic bread wheat (Triticum aestivum L.) and durum wheat (Triticum turgidum L. subsp. durum (Desf.) Husn. ) accessions. Furthermore, B accumulation in two Triticum urartu Tumanian ex Gandilyan and 54 Triticum monococcum L. accessions was measured and showed considerable phenotypic variation. However, B accumulation in these lines was higher than that observed in the most tolerant durum or bread wheats. Mapping of high B tolerance in the durum population AUS14010/Yallaroi revealed a locus possibly allelic to Bo1, a major source of B toxicity tolerance previously identified in bread wheat. Here, we show that the Bo1-specific codominant PCR marker AWW5L7 is predictive of B tolerance status among exotic durum and bread wheat accessions. All tolerant durum accessions assayed carried very similar AWW5L7 marker fragments, indicating wide distribution of this allele among tolerant durum wheats. Three bread wheat accessions had tolerance that was independent of Bo1 and is probably located on chromosome 4A. These lines represent a valuable genetic resource for B toxicity tolerance breeding in wheat.
Rat 3‐Hydroxyanthranilic Acid Oxygenase: Purification from the Liver and Immunocytochemical Localization in the Brain

Abstract: 3‐Hydroxyanthranilic acid oxygenase (3HAO; EC 1.13.11.6). the biosynthetic enzyme of the endogenous excitotoxin quinolinic acid, was purified to homogeneity from rat liver and partially purified from rat brain. The pure enzyme is a single subunit protein with a molecular weight of 37–38.000. Kinetic analyses of both pure liver and partially purified brain 3HAO revealed an identical Km of 3 μM for the substrate 3‐hydroxyanthranilic acid. Evidence for the identity of liver and brain 3HAO was further provided by physicochemical (electrophoretic behavior, heat sensitivity) and biochemical (pH dependency, activation by Fe2+) means. Antibodies were produced against the pure liver enzyme and the identity of liver and brain 3HAO substantiated immunologically in immunotitration and Ouchterlony double‐diffusion experiments. Immuhohistochemical studies using purified anti‐rat 3HAO antibodies were performed on tissue sections of perfused brains and demonstrated a preferential staining of astroglial cells. Notably, the cellular localization of 3HAO in the brain appears to be in part distinct from that of quinolinic acid phosphoribosyltransferase, the catabolic enzyme of quinolinic acid. Pure rat 3HAO and its antibodies can be expected to constitute useful tools for the further elucidation of the brain's quinolinic acid system.
Panniculitis in Patients Treated With BRAF Inhibitors: A Case Series

Abstract:Panniculitis is a rare complication of BRAF inhibitor therapy that is used to treat patients with BRAF-mutated metastatic melanoma. We present a clinicopathologic review of 9 patients who developed panniculitis while on BRAF inhibitor therapy. In 13% of patients on vemurafenib, 3% of patients on dabrafenib and 10% on combination of dabrafenib + trametinib, tender erythematous nodular lesions of panniculitis appeared on legs, arms and trunk. Histological evaluation of 8 biopsies from 7 patients showed predominantly neutrophilic infiltrate in 4, lymphocytic in 1, and mixed in 3. Lesions with neutrophilic infiltrate appeared in earlier stages of treatment than those with mixed or lymphocytic infiltrate. All biopsies showed lobular involvement and 5 also had a septal component. In addition, 1 biopsy had lichenoid inflammation in the epidermis and the other had evidence of vasculitis. Most patients responded to conservative medical management without the need to reduce or to stop BRAF inhibitor therapy. Panniculitis seems to be a rare class effect of BRAF inhibitors that is predominantly lobular and neutrophilic, although other patterns can be seen.
Model‐specification uncertainty in future forest pest outbreak

Climate change will modify forest pest outbreak characteristics, although there are disagreements regarding the specifics of these changes. A large part of this variability may be attributed to model specifications. As a case study, we developed a consensus model predicting spruce budworm (SBW, Choristoneura fumiferana [Clem.]) outbreak duration using two different predictor data sets and six different correlative methods. The model was used to project outbreak duration and the uncertainty associated with using different data sets and correlative methods (=model‐specification uncertainty) for 2011–2040, 2041–2070 and 2071–2100, according to three forcing scenarios (RCP 2.6, RCP 4.5 and RCP 8.5). The consensus model showed very high explanatory power and low bias. The model projected a more important northward shift and decrease in outbreak duration under the RCP 8.5 scenario. However, variation in single‐model projections increases with time, making future projections highly uncertain. Notably, the magnitude of the shifts in northward expansion, overall outbreak duration and the patterns of outbreaks duration at the southern edge were highly variable according to the predictor data set and correlative method used. We also demonstrated that variation in forcing scenarios contributed only slightly to the uncertainty of model projections compared with the two sources of model‐specification uncertainty. Our approach helped to quantify model‐specification uncertainty in future forest pest outbreak characteristics. It may contribute to sounder decision‐making by acknowledging the limits of the projections and help to identify areas where model‐specification uncertainty is high. As such, we further stress that this uncertainty should be strongly considered when making forest management plans, notably by adopting adaptive management strategies so as to reduce future risks.