Miracle-dz/UniFAR-FTUs huggingface dataset:
SUBMILLIMETER ARRAY HIGH-ANGULAR RESOLUTION OBSERVATIONS OF THE MONOCEROS R2 STAR-FORMING CLUSTER

We present the first high-angular resolution study of the MonR2 star-forming complex carried out with the Submillimeter Array at (sub-)millimeter wavelengths. We image the continuum and molecular line emission toward the young stellar objects in MonR2 at 0.85 and 1.3 mm, with resolutions ranging from 0. ″ 5 to ∼3″. While free–free emission dominates the IRS1 and IRS2 continuum, dust thermal emission prevails for IRS3 and IRS5, giving envelope masses of ∼0.1–0.3 M ⊙ ? > . IRS5 splits into at least two sub-arcsecond scale sources, IRS5B and the more massive IRS5A. Our 12CO(2-1) images reveal 11 previously unknown molecular outflows in the MonR2 clump. Comparing these outflows with known IR sources in the IRS5 and IRS3 subclusters allows for tentative identification of driving stars. Line images of molecular species such as CH3CN or CH3OH show that, besides IRS3 (a well-known hot molecular core), IRS5 is also a chemically active source in the region. The gas excitation temperature derived from CH3CN lines toward IRS5 is 144 ± 15 K, indicating a deeply embedded protostar at the hot-core evolutionary stage. Spectral energy distribution fitting of IRS5 gives a mass of ∼7 M ⊙ ? > and a luminosity of 300 L ⊙ ? > for the central source. The derived physical properties of the CO outflows suggest that they contribute to the turbulent support of the MonR2 complex and to the gas velocity dispersion in the clump’s center. The detection of a large number of CO outflows widespread across the region supports the competitive accretion scenario as origin of the MonR2 star cluster.
Structuring Product Models to Facilitate Design Manipulations

In this paper, we analyse the problems associated with product models in computer–based support systems for conceptual design of mechatronic systems. Basically, current product models fail in the sense that they seriously limit the designer in possibilities for making and modifying a description of the system under design. Based on the analysis, we propose a product model structure that is more suitable in this respect.
“I ‘feel’ like I am at university even though I am online.” Exploring how students narrate their engagement with higher education institutions in an online learning environment

This article outlines a collaborative study between higher education institutions in Australia, which qualitatively explored the online learning experience for undergraduate and postgraduate students. The project adopted a narrative inquiry approach and encouraged students to story their experiences of this virtual environment, providing a snapshot of how learning is experienced by those undertaking online studies. The study explores what impacted upon students’ engagement in this environment and how different facets of their learning experience made a qualitative difference to how individuals enacted engagement. Drawing upon Sharon Pittaway’s engagement framework, the article seeks to foreground student voice as the learners define their engagement in learning, the strategies they employed to assist this process and how engagement was enacted at an individual level. The students’ reflections presented in this article can be used to inform teaching and learning strategies designed to improve engagement in the online environment within the higher education sector.
Transplantation of Microglia in the Area of Spinal Cord Injury in an Acute Period Increases Tissue Sparing, but Not Functional Recovery

Microglial cells are known as important mediators of inflammation and immune response in the central nervous system (CNS). However, a neuroprotective role of these cells in post-traumatic processes should not be overlooked. Microglial cells are the first to respond to CNS injury and are further involved in all critical events of pathogenesis. When activated microglia clear the cellular debris and release anti- and proinflammatory cytokines and chemokines, nitric oxide, neurotrophins, and antioxidants capable of producing both neurotoxic and neuroprotective effects. The aim of this study was to determine to what extent the phagocytic activity of microglia in an acute period of spinal cord injury (SCI) in rats can effect the post-traumatic processes. For this purpose we implanted genetically modified Ad5-EGFP or Ad5-GDNF microglial cells into the area of acute SCI. Our experiments demonstrate that the area of intact tissue was lower in the group transplanted with Ad5-GDNF-transduced microglial cells with reduced phagocytic activity than that in the group of animals transplanted with Ad5-EGFP-transduced microglia cells which did not affect the cell activity. At the same time, there was no significant difference in the functional recovery index between these groups. Thus, the increased number of microglia cells with good phagocytic activity in the area of acute SCI may contribute to the improved nervous tissue integrity without a significant effect on the functional recovery within 30 days after injury.
Naïve CD8+ T-Cells Engage a Versatile Metabolic Program Upon Activation in Humans and Differ Energetically From Memory CD8+ T-Cells

Background: Characterization of the intracellular biochemical processes that regulate the generation and maintenance of effector and memory CD8+ T-cells from naïve precursors is essential for our understanding of adaptive immune responses and the development of immunotherapies. However, the metabolic determinants of antigen-driven activation and differentiation remain poorly defined, especially in humans. Methods: We used a variety of different approaches, including gene expression profiling and measurements of nutrient flux, to characterize the basal and activation-induced energetic requirements of naïve and phenotypically-defined subsets of human memory CD8+ T-cells. Findings: Profound metabolic differences were apparent as a function of differentiation status, both at rest and in response to stimulation via the T cell receptor (TCR). Of particular note, resting naïve CD8+ T cells were largely quiescent, but rapidly upregulated diverse energetic pathways after ligation of surface-expressed TCRs. Moreover, autophagy and the mechanistic target of rapamycin (mTOR)-dependent glycolytic pathway were identified as critical mediators of antigen-driven priming in the naïve CD8+ T cell pool, the efficiency of which was dampened by the presence of neutral lipids and fatty acids. Interpretation: These observations provide a metabolic roadmap of the CD8+ T-cell compartment in humans and reveal potentially selective targets for novel immunotherapies.
Kindergarten and New Entrant Teachers' Beliefs and Practices in Mathematics Teaching and Learning

A difference between the beliefs and practices about mathematics learning and teaching held by teachers in kindergartens and teachers in new entrant classrooms may lead to problems as teachers and children manage the transition from kindergarten to school. The New Zealand education system is governed by various curriculum documents underpinned by similar philosophies to support teacher practice. These documents are based on an expectation that teachers build from children’s existing knowledge and experiences. The system is based on expectations relating to knowledge that are loosely linked to the year level and age of children. Evidence about teachers’ practices and beliefs was gathered using a case study approach. Five primary schools and their neighbouring kindergartens were purposively selected for this study. Fourteen kindergarten and five new entrant teachers were observed and interviewed and completed questionnaires about their beliefs and practices in mathematics teaching and learning over a two year period. Mathematical events were analysed to document teachers’ practices. Kindergarten teachers’ practice most commonly mirrored their stated beliefs about how children best learned mathematics. New entrant teachers, although possessing similar stated beliefs to kindergarten teachers, did not necessarily follow these beliefs in their classroom practice. The beliefs of kindergarten and new entrant teachers in mathematics teaching and learning were similar, but their practices differed in several dimensions. Kindergarten teachers believed children best learned mathematics through free play and exploration of the world around them. These teachers encouraged children to follow their own interests and supported children as they developed their ideas and became active problem solvers. These beliefs arose from teachers’ personal experiences combined with the primarily sociocultural focus of the early childhood curriculum, Te Whariki. New entrant teachers’ beliefs about mathematics learning and teaching were similar to those of kindergarten teachers – children learned through having fun, solving problems, and doing things in a real-life context. However, new entrant teachers also had clear expectations of the skills five-year-olds needed to know on their entry to school. When children did not appear to possess these skills, teachers focused on remediation of the perceived deficits. In practice, new entrant teachers directed children’s learning based on their beliefs about what children needed to know to be successful at school. This approach lead to structured and managed environments with little regard for children’s prior-to-school knowledge and experiences. These inconsistencies in mathematics teaching and learning between schools and kindergartens could be minimised with professional development for teachers that focused on developing the relationships between teachers in both settings.
Detecting Selective Protein Binding Inside Plasmonic Nanopores: Toward a Mimic of the Nuclear Pore Complex

Biosensors based on plasmonic nanostructures offer label-free and real-time monitoring of biomolecular interactions. However, so do many other surface sensitive techniques with equal or better resolution in terms of surface coverage. Yet, plasmonic nanostructures offer unique possibilities to study effects associated with nanoscale geometry. In this work we use plasmonic nanopores with double gold films and detect binding of proteins inside them. By thiol and trietoxysilane chemistry, receptors are selectively positioned on the silicon nitride interior walls. Larger (~150 nm) nanopores are used detect binding of averaged sized proteins (~60 kg/mol) with high signal to noise (>100). Further, we fabricate pores that approach the size of the nuclear pore complex (diameter down to 50 nm) and graft disordered phenylalanine-glycine nucleoporin domains to the walls, followed by titration of karyopherinβ1 transport receptors. The interactions are shown to occur with similar affinity as determined by conventional surface plasmon resonance on planar surfaces. Our work illustrates another unique application of plasmonic nanostructures, namely the possibility to mimic the geometry of a biological nanomachine with integrated optical sensing capabilities.
Rodent-Mediated Seed Dispersal Shapes Species Composition and Recruitment Dynamics in Ecotones

Ecotones are considered unique environments, and the concepts of edge effects and ecotonal species have been applied widely. Our understanding of the mechanisms that underlie population and community responses to edge effects has been advanced by recent studies. However, little evidence exists to support an increased density and species richness in ecotones regarding rodent-mediated seed dispersal in response to edge plots between communities. Pinus armandii and Quercus variabilis communities are typical of the Qinling Mountains, China. To elucidate what shapes tree species composition and recruitment dynamics in ecotones, we compared the differences in secondary and tertiary seed dispersal as well as predation in pine and oak by scatter-hoarding rodents as well as the regeneration characteristics of both species in their ecotones with different plots (i.e., 5–8, 15–18, and 27–30 m widths) in the eastern Qinling Mountains. We found that the seeds of pine and oak were removed rapidly, with no differences in the seed removal rates in their ecotones with different plots. Moreover, 13.0 and 36.0% of the scatter hoards of pine and oak, respectively, were established by small rodents in ecotones with a width of 5–8 m, and 3.67 and 7.33% in ecotones with a width of 27–30 m. The seedling densities of pine and oak were significantly higher in ecotones at widths of 5–8 m compared with widths of 15–18 and 27–30 m. According to the seed dispersal and seedling recruitment patterns of pine and oak, the disproportionate abundance of seedlings in ecotones may be due at least partly to patterns of seed caching by rodents.
Quantitative Data From Rating Scales: An Epistemological and Methodological Enquiry

Rating scales are popular methods for generating quantitative data directly by persons rather than automated technologies. But scholars increasingly challenge their foundations. This article contributes epistemological and methodological analyses of the processes involved in person-generated quantification. They are crucial for measurement because data analyses can reveal information about study phenomena only if relevant properties were encoded systematically in the data. The Transdisciplinary Philosophy-of-Science Paradigm for Research on Individuals (TPS-Paradigm) is applied to explore psychological and social-science concepts of measurement and quantification, including representational measurement theory, psychometric theories and their precursors in psychophysics. These are compared to theories from metrology specifying object-dependence of measurement processes and subject-independence of outcomes as key criteria, which allow tracing data to the instances measured and the ways they were quantified. Separate histories notwithstanding, the article’s basic premise is that general principles of scientific measurement and quantification should apply to all sciences. It elaborates principles by which these metrological criteria can be implemented also in psychology and social sciences, while considering their research objects’ peculiarities. Application of these principles is illustrated by quantifications of individual-specific behaviors (‘personality’). The demands rating methods impose on data-generating persons are deconstructed and compared with the demands involved in other quantitative methods (e.g., ethological observations). These analyses highlight problematic requirements for raters. Rating methods sufficiently specify neither the empirical study phenomena nor the symbolic systems used as data nor rules of assignment between them. Instead, pronounced individual differences in raters’ interpretation and use of items and scales indicate considerable subjectivity in data generation. Together with recoding scale categories into numbers, this introduces a twofold break in the traceability of rating data, compromising interpretability of findings. These insights question common reliability and validity concepts for ratings and provide novel explanations for replicability problems. Specifically, rating methods standardize only data formats but not the actual data generation. Measurement requires data generation processes to be adapted to the study phenomena’s properties and the measurement-executing persons’ abilities and interpretations, rather than to numerical outcome formats facilitating statistical analyses. Researchers must finally investigate how people actually generate ratings to specify the representational systems underlying rating data.
Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells

The extracellular vesicles (EVs) released by Leishmania can contribute to the establishment of infection and host immunomodulation. In this study, we characterized the shedding of EVs from Leishmania (Leishmania) amazonensis promastigotes. This species is the causative agent of cutaneous leishmaniasis, and its role during interactions with bone marrow-derived macrophages (BMDMs) and peritoneal B-1 cells was evaluated. Leishmania amazonensis promastigotes cultivated in vitro at different times and temperatures spontaneously released EVs. EVs were purified using size-exclusion chromatography (SEC) and quantitated by nanoparticle tracking analysis (NTA). NTA revealed that the average size of the EVs was approximately 180 nm, with concentrations ranging from 1.8 × 108 to 2.4 × 109 vesicles/mL. In addition, the presence of LPG and GP63 were detected in EVs obtained at different temperatures. Naïve BMDMs stimulated with EVs exhibited increased IL-10 and IL-6 expression. However, incubating B-1 cells with parasite EVs did not stimulate IL-10 expression but led to an increase in the expression of IL-6 and TNFα. After 7 weeks post-infection, animals infected with L. amazonensis promastigotes in the presence of parasite EVs had significant higher parasite load and a polarization to Th2 response, as compared to the group infected with the parasite alone. This work demonstrated that EVs isolated from L. amazonensis promastigotes were able to stimulate macrophages and B-1 cells to express different types of cytokines. Moreover, the immunomodulatory properties of EVs probably contributed to an increase in parasite burden in mice. These findings suggest that the functionality of L. amazonensis EVs on immune system favor of parasite survival and disease progression.
Promising Clinical Outcome With Long Term Follow-Up After Body Gamma Knife Stereotactic Radiosurgery for Patients With Early Stage Non-small Cell Lung Cancer

Introduction: Stereotactic ablative radiosurgery (SRS) or stereotactic ablative body radiotherapy (SABR) is the standard treatment for patients with inoperable early stage non-small cell lung cancer (NSCLC), the body gamma knife SRS (ɤ-SRS) is a special SABR technology developed in China. This study prospectively assessed the clinical outcome, toxicity and cost following body ɤ-SRS for early stage NSCLC. Methods: From 2007 to 2010, a total of 29 patients with early stage NSCLC were prospectively enrolled in this study. The prescription dose for Planning Target Volume (PTV), Clinical Target Volume (CTV), and Gross Target Volume (GTV) were 50, 60, and 70 gray (Gy) in 10 fractions. Isodose curves of 50, 60, and 70% covered at least 100% of PTV, 90% of CTV, and 80% of GTV, respectively. The body ɤ-SRS was delivered 5 days per week and completed in 2 weeks. Results: Median follow-up time was 62.0 (range 11.1-140.3) months. 1-, 3-, 5-year OS rates were 93.1%, 72.0%, 60.3%; PFS rates were 86.2, 64.2 and 48.8%; and LR, RR, and DM rates were 10.9%, 21.4%, 29.0%. The median cost of the body ɤ-SRS during treatment was 4,838 (range 4,615–4,923) dollars and the median cost through 5 years was 36,960 (range 9920-56,824) dollars. Conclusion: With existing clinical data, the body ɤ-SRS is an effective treatment option for patients with medically inoperable early stage NSCLC or patients who do not prefer operation, as they may benefit from the minimized toxicity. Due to excellent cost effectiveness, the availability of the body ɤ-SRS will expand, especially in developing nations, and underdeveloped countries.
(–)-Epicatechin Supplementation Inhibits Aerobic Adaptations to Cycling Exercise in Humans

The purpose of the study was to determine if cycling exercise combined with (–)-epicatechin supplementation was more effective at increasing training adaptations than cycling combined with a placebo. Blood and muscle samples were obtained at rest before and after training to determine the effects of (–)-epicatechin supplementation on total serum antioxidant capacity, skeletal muscle mitochondrial protein content, and skeletal muscle myostatin gene expression. Participants (n = 20) completed two testing sessions separated by 4 weeks of cycle training, with supplementation of 100 mg (200 mg total daily) of (–)-epicatechin or a placebo, twice daily. Data were analyzed using a two-way mixed model ANOVA for each variable and the alpha level was set at p ≤ 0.05. A significant increase was observed for time for relative peak anaerobic power (p < 0.01), relative anaerobic capacity (p < 0.01), and fatigue index (p < 0.01). A significant increase was observed for time for absolute peak VO2 (p < 0.01) and peak power output obtained during the peak VO2 test (p < 0.01). A significant interaction between group and time for relative peak VO2 was observed (p = 0.04). Relative peak VO2 significantly increased over time in the placebo group (p < 0.01), but not in the (–)-epicatechin group (p = 0.21). A significant increase was observed for time for total serum antioxidant capacity (p = 0.01). No interaction or main effect of time was observed for myostatin (p > 0.05). Likewise, no interaction or main effect of time was observed for cytochrome C or citrate synthase (p > 0.05). A significant interaction effect was observed for succinate dehydrogenase (SDH; p = 0.02). SDH content increased significantly for the placebo group (p = 0.03, partial η2 = 0.59), but not for the (–)-epicatechin group (p = 0.81). Further, whereas no difference existed between the groups for SDH at baseline (p = 0.23), SDH content was significantly greater in the placebo group at the post time point (p = 0.01). Results indicate that (–)-epicatechin supplementation does not affect myostatin gene expression or anaerobic training adaptations but inhibits aerobic and mitochondrial SDH adaptations to cycle exercise training.
Personalized computational modeling of vascular access creation

To be able to connect haemodialysis patients to an artificial kidney a connection is surgically made between a coronary artery and vein in the arm (AVF). The exact location of this new vessel access is crucial for success. If an AVF is not made on the location that is optimal for the patient, this can result in a too low blood stream, which makes the vessel access not usable for haemodialysis, or, on the opposite, in a too high blood stream which results in heart problems or insufficient blood flow in the hand. Additional interventions are then necessary to realize a usable vessel access. The researchers developed a computer model that can predict the blood stream after placement of an AVF and that can thus help select the optimal location. The computer model was capable of selecting in 16 out of 21 patients before the operation the same vessel access as a very experienced surgeon. The model could be valuable during the determination of the optimal AVF location, especially for an inexperienced surgeon.