Miracle-dz/UniFAR-FTUs huggingface dataset:
Factors Influencing Mental Health Screening and Treatment Among Women in a Rural South Central Appalachian Primary Care Clinic.

PURPOSE Some of the most significant mental health concerns among US adults are depression, anxiety, substance abuse, and intimate partner violence. These concerns represent an ever-growing portion of the primary care population, especially in rural areas. However, few studies have examined factors influencing screening and treatment of these concerns by primary care providers, particularly in Appalachia. This study explores barriers and facilitators to mental health screening and treatment among women at a rural, primary care clinic in Appalachia. METHODS Eighteen patients and 4 providers were interviewed face-to-face. Thematic analysis was used to identify emergent themes. FINDINGS Patients identified 3 barriers (stigma, lack of support, and lack of education) and 2 facilitators (integrated care and positive experiences with providers). Providers identified 4 barriers (operational barriers, mental health competence, predicted patient reactions, and patient attitudes) and 3 facilitators (clinic characteristics, provider characteristics, and patient and provider education). Generally, patients focused more on individual and social factors influencing mental health service use, while providers were more aware of training gaps, logistical factors at the clinic, and systemic issues within the larger health care system. Both participant types emphasized specific interpersonal qualities and the importance of integrated services. CONCLUSIONS Screening and treatment may be influenced by the availability and advertisement of integrated services, institutional support, strong patient-provider relationships, and provider training and experience. For rural south central Appalachia women, limited mental health resources may make these factors even more salient.
Logopenic, mixed, or Alzheimer-related aphasia?

Objective: This study tested the hypothesis that patients with primary progressive aphasia (PPA) who do not meet the proposed criteria for any of the recognized subtypes would have the atrophy pattern reported in the past for logopenic variant PPA (lvPPA), in turn suggesting that the PPA of likely Alzheimer disease origin is more variable than that captured in the current lvPPA diagnostic recommendations. Methods: MRI gray matter volumes from 14 patients with mixed PPA who failed to meet the diagnostic recommendations for any recognized variant were compared with those of 25 matched control participants via voxel-based morphometry. Results: The mixed PPA group had left temporoparietal atrophy with a pattern identical to that in previously reported lvPPA cohorts. Conclusion: Patients with PPA who did not meet the criteria for any recognized PPA variant at their initial assessment had the group-level atrophy pattern previously reported as the hallmark of lvPPA. We suggest that the specific language features proposed for lvPPA are too narrow to characterize the language impairments arising from likely Alzheimer pathology.
Polychromophilus murinus: a malarial parasite of bats: life-history and ultrastructural studies

SUMMARY Polychromophilus murinus, a malaria parasite of Chiroptera is reported from Myotis daubentoni in England. The vector was suspected to be the ectoparasitic Nycteribiid fly, Nycteribia kolenatii. N. kolenatii collected from wild-caught M. daubentoni were found to have oocysts on the midgut and sporozoites in the salivary glands. Wild-caught N. kolenatii were maintained on two wild-caught M. daubentoni harbouring heavy (patent) infections of P. murinus; both oocysts and sporozoites were found in these flies. The mature oocysts measured 52–71 μm in diameter. Sporozoites were straight or slightly crescentic and had a mean length of 7·4 μm. Electron microscopy of immature and mature oocysts revealed a morphology similar to that of malaria parasites. Sporozoites were also similar in structure to Plasmodium sporozoites and were found in the epithelial cells of the salivary gland and within the lumen; a cytostome was present and transverse sections revealed 21 microtubules arranged evenly around the periphery. Sporozoites were observed within the basement membrane of the salivary gland of N. kolenatii; such sporozoites appeared to be penetrating the gland, a process hitherto not described in malaria parasites. Rickettsia-like bodies were found within the cytoplasm of the epithelial cells of the salivary gland. Exflagellation of microgametocytes was achieved. An ultrastructural study of the gametocytes revealed a structure similar to that described in other Haemoproteidae. A common feature of infected erythrocytes was a projecting erythrocyte membrane. Attempts to find schizogony in impression smears and sections of tissues of two infected M. daubentoni were not successful.
Effect of Green Tea Aqueous Extract on Body Weight, Glucose Level, and Kidney Functions in Diabetic Male Albino Rats

The effect of daily oral doses of green tea water extract for 4 weeks on body weight, plasma glucose level, kidney functions in alloxan-induced diabetic rats was investigated. Forty adult male albino rats (150 -160 g) was divided into 4 experimental groups: The first group was considered as control group. The second group was treated with water extract of the green tea (200 mg/kg b.w). The third group was infected by induction of experimental diabetes by intraperitonial injection of (150 mg/kg b.w) alloxan, and the fourth group was infected by induction diabetes and treated with green tea extract. Different physiological parameters were performed including recording of the body weight and measuring blood sugar, creatinine and urea levels. Body weight loses, plasma glucose, creatinine, and urea levels were significantly (p< 0.05) reduced in diabetic rats treated with green tea when compared with the diabetic rats. From these results, it can be conclude that the consumption of green tea extract produced a significant reduction in glucose level in diabetic rats. In addition, it is capable of improving the impaired kidney functions in diabetic animals.
Force generation in muscle: time‐resolved measurements of atpase activity using a phosphate‐sensitive fluorophore

The speed and efficiency of chemomechanical transduction appear to be well matched to contractile function in muscle and other cell types. When active, the contractile proteins myosin and actin convert chemical energy from the hydrolysis of ATP into mechanical work, a process that involves sequential dissociation of the products of ATP hydrolysis from the actomyosin complex. An improved understanding of chemomechanical transduction has emerged only slowly from experimental study, since it is difficult to perform biochemical measurements in an intact contractile system, and it is virtually impossible to reliably place mechanical loads on contractile protein biochemical assay systems in solution. These are important limitations, since it is probable that the transitional rate constants of one or more steps in the ATPase cycle vary with mechanical stress or strain. In recent years, the use of chemically caged compounds such as ATP, Ca¥ and inorganic phosphate (Pé) has circumvented these limitations to a significant degree: rapid photolysis of caged compounds can be used to probe force-generating transitions in both skeletal and cardiac muscle. For example, studies with caged Pé now suggest that force development in muscle involves one or more cross-bridge transitions (most probably an isomerization) kinetically coupled to Pé release (Dantzig et al. 1992).
Real-Time Barcode Detection and Classification using Deep Learning

: Barcodes, in their different forms, can be found on almost any packages available in the market. Detecting and then decoding of barcodes have therefore great applications. We describe how to adapt the state-of-the-art deep learning-based detector of You Only Look Once (YOLO) for the purpose of detecting barcodes in a fast and reliable way. The detector is capable of detecting both 1D and QR barcodes. The detector achieves state-of-the-art results on the benchmark dataset of Muenster BarcodeDB with a detection rate of 0.991. The developed system can also ﬁnd the rotation of both the 1D and QR barcodes, which gives the opportunity of rotating the detection accordingly which is shown to beneﬁt the decoding process in a positive way. Both the detection and the rotation prediction shows real-time performance.
Estrogen therapy and risk of cardiovascular events among women with type 2 diabetes.

OBJECTIVE To evaluate the association between estrogen therapy and cardiovascular disease risk among women with type 2 diabetes. RESEARCH DESIGN AND METHODS A retrospective, case-cohort study was conducted among 6017 women aged 45-80 years with type 2 diabetes from 1 January 1986 to 31 December 1992 at the Group Health Cooperative in Washington state. Cardiovascular outcomes, including nonfatal myocardial infarction (n = 215), coronary revascularization (n = 253), and cardiovascular deaths (n = 229), were ascertained through 31 December 1998. Use of estrogen and progestin was derived from automated pharmacy records and modeled as a time-dependent variable. Median follow-up was 6.8 years. Multivariable-adjusted relative risk (RR) and 95% CI were calculated using Cox proportional hazard models for case-cohort analyses. RESULTS Current use of estrogen with (RR 0.43, 95% CI 0.22-0.85) or without (0.48, 0.30-0.78) progestin was associated with a decreased risk of cardiovascular events compared with never having used estrogen. Risk of cardiovascular events associated with a first episode of estrogen use (with or without progestin) of <25 months' duration (1.12, 0.49-2.54), first episode of use >or=25 months' duration (0.32, 0.06-1.70), and current use that was not the first episode of use (0.42, 0.42-0.67) indicated that recent initiation was not associated with an increase or decrease in risk. CONCLUSIONS These results show an association of estrogen therapy, with or without progestin, with decreased risk of cardiovascular events among women with type 2 diabetes. This association should be further investigated in large randomized, controlled trials.
The "loss" of centromeres from chromosomes of aged women.

Both aneuploid cells in mitosis and nondisjunction in meiosis increase with advancing age. The cause(s) of these phenomena remains unknown. Here, it was confirmed that a positive Cd-band reflects the presence of a functioning centromere while a negative reaction is indicative of its inactivation or loss. We applied the Cd-banding technique to mitotic spreads obtained from 14 aged and 13 control females (peripheral blood culture). Of 6,474 scored chromosomes from the aged women, 62(0.96%) were Cd-negative; this was the case in 12 of 3,861 (0.31%) chromosomes from the younger controls. The difference was highly significant (P less than .001). About 60% of the 62 Cd-negative chromosomes from aged women belong to the C group. Chromosomes from six of the 14 aged females were additionally examined by either the C-band or the distamycin-DAPI technique; of 2,080 chromosomes, 13 (0.63%) showed premature separation of their centromeres (control: 7/5, 080, 0.14%). Our findings suggest that in aged women, the chromosomes tend to lose their Cd-positive material and the function of the centromere.
Bacterial biofilm diversity in contact lens-related disease: emerging role of Achromobacter, Stenotrophomonas, and Delftia.

PURPOSE Multi-species biofilms associated with contact lens cases and lenses can predispose individuals to contact lens-related inflammatory complications. Our study used culture-independent methods to assess the relationship between the severity of contact lens-related disease and bacteria residing in biofilms of contact lens cases and lenses. METHODS Contact lens cases and lenses from 28 patients referred to the West Virginia University Eye Institute and diagnosed as having mild keratitis, keratitis with focal infiltrates, or corneal ulcers were processed and evaluated for bacterial composition based on 16S ribosomal RNA gene sequencing. Cases and lenses from nine asymptomatic contact lens wearers were processed in a manner similar to controls. Relationships between disease severity, bacterial types, and bacterial diversity were evaluated statistically. RESULTS Disease severity and presenting visual acuity correlated with an increase in the diversity of bacterial types isolated from contact lens cases. A significant difference also was observed in the number of bacterial types associated with the three clinical groups. Achromobacter, Stenotrophomonas, and Delftia were prevalent in all disease groups, and Achromobacter and Stenotrophomonas were present in one asymptomatic control. Scanning electron microscopy revealed that Achromobacter and Stenotrophomonas formed a biofilm on the surface of contact lenses. CONCLUSIONS Culture-independent methods identified an association between disease severity and bacterial diversity in biofilms isolated from cases and lenses of patients with contact lens-related corneal disease. Achromobacter, Stenotrophomonas, and Delftia were predominant bacteria identified in our study, drawing attention to their emerging role in contact lens-related disease.
Self-Esteem and Body Image Perception in a Sample of University Students

Problem Statement: This cross-sectional study was conducted to determine the relationship established between self-esteem and body image dissatisfaction, as subjective variables among young, female Romanian university students. Purpose of Study: We hypothesize that young women’s body dissatisfaction is related to their self-esteem level. The second purpose of this study is to verify whether self-esteem level and body size acceptance, which are subjective variables, are significantly correlated with BMI as an objective variable. Subsequently, we intend to estimate which range self-esteem in our research group is determined by self-perception of the body and what role BMI plays in this equation. Methods: The data were collected using measurements and questionnaires from a random sample of 160 female students (19-21 y.o.) assumed to be healthy and educated, with constant physical activity and having an urban lifestyle. Using descriptive statistics for each variable, we analyzed summaries of the sample and the collected data. For pares of variables we calculated the Pearson correlation coefficient (r), and we tested its statistical significance using the “t” test. Findings and Results: The BMI mean value – 20.93 kg/cm2 (SD = 3.30) is equivalent to a slander figure, corresponding to an average height of 1.65 m (SD = 0.06) and an average weight of ~ 57 kg (56.99 kg; SD = 9.70). A prevalence of body dissatisfaction was reported, with 79% of girls reporting being unpleased with their physical appearance. Self-esteem and body dissatisfaction were in a significant negative correlation: r (158) = - 0.36, p< . 0005. We found a consistent, statistically significant correlation between BMI and body dissatisfaction (r (158) = 0.56, p< . 0005). An important part (31%) of body dissatisfaction is determined by BMI and subsequently by weight and fat deposits. Conclusions and Recommendations: Due the statistical correlations calculated for self-esteem, body image dissatisfaction and BMI, we can conclude that selfesteem is better correlated with other subjective parameters (body image) than with objective and relatively stable measurements (BMI in our research). BMI values are useful predictors of body dissatisfaction risk among young females. Physical activity results in certain improvements in the physical and psychological parameters researched in the present paper. Therefore, we made some recommendations regarding motivation, exercise enjoyment and communication techniques aimed at promoting physical activities among young women. Key words: Weight; body dissatisfaction; cultural patterns; physical activity; self-image distortion.
Excessive Autophagy Contributes to Neuron Death in Cerebral Ischemia

Aims: To determine the extent to which autophagy contributes to neuronal death in cerebral hypoxia and ischemia. Methods: We performed immunocytochemistry, western blot, cell viability assay, and electron microscopy to analyze autophagy activities in vitro and in vivo.Results: In both primary cortical neurons and SH‐SY5Y cells exposed to oxygen and glucose deprivation (OGD)for 6 h and reperfusion (RP) for 24, 48, and 72 h, respectively, an increase of autophagy was observed as determined by the increased ratio of LC3‐II to LC3‐I and Beclin‐1 (BECN1) expression. Using Fluoro‐Jade C and monodansylcadaverine double‐staining, and electron microscopy we found the increment in autophagy after OGD/RP was accompanied by increased autophagic cell death, and this increased cell death was inhibited by the specific autophagy inhibitor, 3‐methyladenine. The presence of large autolysosomes and numerous autophagosomes in cortical neurons were confirmed by electron microscopy. Autophagy activities were increased dramatically in the ischemic brains 3–7 days postinjury from a rat model of neonatal cerebral hypoxia/ischemia as shown by increased punctate LC3 staining and BECN1 expression. Conclusion: Excessive activation of autophagy contributes to neuronal death in cerebral ischemia.
Role of Nitric Oxide in Regulation of Brain Stem Circulation during Hypotension

We tested the hypothesis that nitric oxide (NO) plays a role in CBF autoregulation in the brain stem during hypotension. In anesthetized rats, local CBF to the brain stem was determined with laser-Doppler flowmetry, and diameters of the basilar artery and its branches were measured through an open cranial window during stepwise hemorrhagic hypotension. During topical application of 10−5 mol/L and 10−4 mol/L Nω-nitro-L-arginine (L-NNA), a nonselective inhibitor of nitric oxide synthase (NOS), CBF started to decrease at higher steps of mean arterial blood pressure in proportion to the concentration of L-NNA in stepwise hypotension (45 to 60 mm Hg in the 10−5 mol/L and 60 to 75 mm Hg in the 10−4 mol/L L-NNA group versus 30 to 45 mm Hg in the control group). Dilator response of the basilar artery to severe hypotension was significantly attenuated by topical application of L-NNA (maximum dilatation at 30 mm Hg: 16 ± 8% in the 10−5 mol/L and 12 ± 5% in the 10−4 mol/L L-NNA group versus 34 ± 4% in the control group), but that of the branches was similar between the control and L-NNA groups. Topical application of 10−5 mol/L 7-nitro indazole, a selective inhibitor of neuronal NOS, did not affect changes in CBF or vessel diameter through the entire pressure range. Thus, endothelial but not neuronal NO seems to take part in the regulation of CBF to the the brain stem during hypotension around the lower limits of CBF autoregulation. The role of NO in mediating dilatation in response to hypotension appears to be greater in large arteries than in small ones.
Pharmacokinetics of Sublingual Sufentanil Tablets and Efficacy and Safety in the Management of Postoperative Pain

Background and Objectives A sublingual sufentanil tablet is being developed as a potential alternative to intravenous (IV) opioids for the management of postoperative pain. The objective of these studies was to evaluate the pharmacokinetics, efficacy, and safety of sublingual sufentanil tablets for postoperative pain management. Methods The pharmacokinetics of sublingual sufentanil 10 and 80 µg were compared with IV sufentanil in 12 subjects in a phase 1 study. The safety and efficacy of sublingual sufentanil (5–15 µg) were evaluated in double-blind, randomized, placebo-controlled phase 2 studies in patients undergoing knee replacement surgery (n = 101) or open abdominal (ABD) surgery (n = 94). The primary efficacy measurement was the summed pain intensity difference compared with baseline over 12 hours (SPID-12). Results Sublingual sufentanil pharmacokinetics were dose proportional following single doses of 10 and 80 µg. Plasma half-time (time from peak plasma concentration to 50% of peak concentration) was 80 to 90 minutes for sublingual sufentanil compared with 15 minutes or less for IV sufentanil. In the phase 2 studies, greater SPID-12 scores (ie, lower pain intensity) compared with placebo were observed for sublingual sufentanil 15 µg in the knee replacement study (P < 0.05) and for 10 and 15 µg in the ABD study (P < 0.01). All doses of sublingual sufentanil were well tolerated, and the incidence of adverse events was similar between the sublingual sufentanil and placebo groups. Conclusions Sufentanil formulated as a sublingual solid dosage form provides a duration of action that allows effective analgesia for postoperative patients in a medically supervised setting.
Polyunsaturated fatty acid supplementation for schizophrenia.

BACKGROUND Limited evidence supports a hypothesis suggesting that schizophrenic symptoms may be the result of altered neuronal membrane structure and metabolism. The structure and metabolism is dependent on blood plasma levels of certain essential fatty acids and their metabolites. OBJECTIVES To review the effects of polyunsaturated fatty acids for people with schizophrenia. SEARCH STRATEGY We have updated the initial searches of 1998 and 2002 (Cochrane Schizophrenia Group's Register, July 2005), and where necessary, we contacted authors and relevant pharmaceutical companies. SELECTION CRITERIA We included all randomised clinical trials of polyunsaturated fatty acid treatment for schizophrenia. DATA COLLECTION AND ANALYSIS Working independently, we selected studies for quality assessment and extracted relevant data. We analysed on an intention-to-treat basis. Where possible and appropriate we calculated the Relative Risk (RR) and their 95% confidence intervals (CI) and estimated the number needed to treat (NNT). For continuous data we calculated weighted mean differences (WMD) and their 95% confidence intervals. We also inspected the data for heterogeneity. MAIN RESULTS When any dose omega-3 (E-EPA or EPA) is compared with placebo, small short trials suggest that the need for neuroleptics appears to be reduced for people allocated omega-3 supplementation (n=30, 1 RCT, RR 0.73 CI 0.54 to 1.00) and mental state may improve (n=30, 1 RCT, RR not gaining 25% change in PANSS scores 0.54 CI 0.30 to 0.96, NNT3 CI 2-29). There are no differences in the number of people leaving the study early (n=271, 4 RCTs, RR 0.91 CI 0.36 to 2.33). There are few data on the comparison of any dose omega-6 (GLA) with placebo. For movement disorder outcomes, the only small study we found does not show any difference for average short-term endpoint AIMS score (n=16, 1 RCT, MD 1.30 CI -1.96 to 4.56). When any dose omega 3 (E-EPA or EPA) is compared with any dose omega-3 (DHA) there is no clear difference for mental state outcome of not gaining 25% change in PANSS scores (n=31, 1 RCT, RR 0.66 CI 0.39 to 1.11). When different doses of omega-3 (E-EPA) are compared with placebo there are no differences in measures of global and mental state between the studies. For the outcome of 'experiencing at least one adverse effect' no differences between groups are found for any dose (1g/day E-EPA vs placebo n=63 1 RCT, RR 0.97 CI 0.60 to 1.56; 2g/day E-EPA vs placebo n=63 1 RCT, RR 0.67 CI 0.37 to 1.20; 4g/day E-EPA vs placebo n=58, 1 RCT, RR 1.15 CI 0.72 to 1.82). AUTHORS' CONCLUSIONS Two updates of this review have resulted in more included studies but relatively little useful additional data. The results remain inconclusive. The new trials all compare the omega-3 polyunsaturated fatty acids, in particular eicosapentaenoic acid and its ester, ethyl-eicosapentaenoic acid. The use of omega-3 polyunsaturated fatty acids for schizophrenia still remains experimental and this review highlights the need for large well designed, conducted and reported studies.