Miracle-dz/UniFAR-FTUs huggingface dataset:
Fracture Resistance of Additively Manufactured Zirconia Crowns when Cemented to Implant Supported Zirconia Abutments: An in vitro Study.

PURPOSE To compare the fracture resistance of implant-supported milled zirconia, milled lithium disilicate, and additively manufactured zirconia crowns. MATERIALS AND METHODS Maxillary cast with a dental implant replacing right second bicuspid was obtained. Custom abutments and full-contour crowns for milled zirconia, milled lithium disilicate, and additively manufactured zirconia crowns (n = 10/group) were digitally designed and fabricated. The crowns were cemented to implant supported zirconia abutments and mounted onto polyurethane blocks. Fracture resistance was determined by vertical force application using a universal testing machine at a crosshead speed of 2mm/minute. Kruskal-Wallis test was used to analyze data and failure mode was determined for all the groups. RESULTS Milled zirconia crowns demonstrated the highest median fracture resistance (1292±189 N), followed by milled lithium disilicate (1289±142 N) and additively manufactured zirconia (1243.5±265.5 N) crowns. Statistical analysis showed no significant differences in fracture resistance between the groups (p = 0.4). All specimens fractured at the implant-abutment interface. CONCLUSION Additively manufactured zirconia crowns demonstrated similar fracture resistance to milled ceramic crowns, when cemented to implant supported zirconia abutments. The results of this in vitro study signify the promising potential of additive manufacturing for the fabrication of all ceramic zirconia crowns. This article is protected by copyright. All rights reserved.
Reaction of Electrons with DNA: Radiation Damage to Radiosensitization

This review article provides a concise overview of electron involvement in DNA radiation damage. The review begins with the various states of radiation-produced electrons: Secondary electrons (SE), low energy electrons (LEE), electrons at near zero kinetic energy in water (quasi-free electrons, (e−qf)) electrons in the process of solvation in water (presolvated electrons, e−pre), and fully solvated electrons (e−aq). A current summary of the structure of e−aq, and its reactions with DNA-model systems is presented. Theoretical works on reduction potentials of DNA-bases were found to be in agreement with experiments. This review points out the proposed role of LEE-induced frank DNA-strand breaks in ion-beam irradiated DNA. The final section presents radiation-produced electron-mediated site-specific formation of oxidative neutral aminyl radicals from azidonucleosides and the evidence of radiosensitization provided by these aminyl radicals in azidonucleoside-incorporated breast cancer cells.
Intradermal or Sublingual Delivery and Heat-Labile Enterotoxin Proteins Shape Immunologic Responses to a CFA/I Fimbria-Derived Subunit Antigen Vaccine against Enterotoxigenic Escherichia coli

Enterotoxigenic Escherichia coli (ETEC) is a major cause of infectious diarrhea in children, travelers, and deployed military personnel. As such, development of a vaccine would be advantageous for public health. One strategy is to use subunits of colonization factors combined with antigen/adjuvant toxoids as an ETEC vaccine. ABSTRACT Enterotoxigenic Escherichia coli (ETEC) is a major cause of infectious diarrhea in children, travelers, and deployed military personnel. As such, development of a vaccine would be advantageous for public health. One strategy is to use subunits of colonization factors combined with antigen/adjuvant toxoids as an ETEC vaccine. Here, we investigated the intradermal (i.d.) or sublingual (s.l.) delivery of CFA/I fimbrial antigens, including CfaEB and a CfaE-heat-labile toxin B subunit (LTB) chimera admixed with double mutant heat-labile toxin (LT) LT-R192G/L211A (dmLT). In addition, we compared dmLT with other LT proteins to better understand the generation of adjuvanted fimbrial and toxoid immunity as well as the influence on any local skin reactogenicity. We demonstrate that immunization with dmLT admixed with CfaEB induces robust serum and fecal antibody responses to CFA/I fimbriae and LT but that i.d. formulations are not optimal for s.l. delivery. Improved s.l. vaccination outcomes were observed when higher doses of dmLT (1 to 5 μg) were admixed with CfaEB or, even better, when a CfaE-LTB chimera antigen was used instead. Serum anti-CFA/I total antibodies, detected by enzyme-linked immunosorbent assay, were the best predictor of functional antibodies, based on the inhibition of red blood cell agglutination by ETEC. Immunization with other LT proteins or formulations with altered B-subunit binding during i.d. immunization (e.g., by addition of 5% lactose, LTA1, or LT-G33D) minimally altered the development of antibody responses and cytokine recall responses but reduced skin reactogenicity at the injection site. These results reveal how formulations and delivery parameters shape the adaptive immune responses to a toxoid and fimbria-derived subunit vaccine against ETEC.
The Role of Vitamin D Receptor Gene Polymorphisms in Thyroid-Associated Orbitopathy

ABSTRACT Purpose: Thyroid-associated orbitopathy (TAO) is an autoimmune disease that typically occurs in the course of Graves’ disease. VDR gene has been tested for its association with autoimmune thyroid diseases, with conflicting results. The study aimed to evaluate the association of selected VDR polymorphisms (rs2228570, rs1544410, rs7975232, rs731236, and rs11568820) with susceptibility to TAO. Methods: 108 TAO patients and 130 control subjects were enrolled. Polymorphisms were studied by PCR-RFLP or TaqMan real-time PCR. Results: Genotype distributions of rs2228570 differed significantly between TAO and controls under a dominant model (OR = 2.05; 95% CI: 1.03–4.08; p = .04). TAO patients also had slightly increased frequency of C allele of rs2228570 comparing to controls (p = .05). However, the study failed to find any associations between VDR polymorphisms and the analyzed clinical features of the disease. Conclusions: These preliminary results have shown that C allele of rs2228570 may contribute to the development of TAO in patients of Caucasian Polish origin.
Further characterization of a novel EP2 and EP3 receptor dual agonist, ONO‐8055, on lower urinary tract function in normal and lumbar canal stenosis rats

To further explore the effects of a novel EP2 and EP3 dual agonist, ONO‐8055, on detrusor contractility, we investigated the responses of bladder strips from sham and lumbar canal stenosis (LCS) rats to this agonist, its effects on lower urinary tract function in normal rats, and mRNA expression of EP2 and EP3 receptors in the sham and LCS rats.
An Efficient Three-Dimensional Convolutional Neural Network for Inferring Physical Interaction Force from Video

Interaction forces are traditionally predicted by a contact type haptic sensor. In this paper, we propose a novel and practical method for inferring the interaction forces between two objects based only on video data—one of the non-contact type camera sensors—without the use of common haptic sensors. In detail, we could predict the interaction force by observing the texture changes of the target object by an external force. For this purpose, our hypothesis is that a three-dimensional (3D) convolutional neural network (CNN) can be made to predict the physical interaction forces from video images. In this paper, we proposed a bottleneck-based 3D depthwise separable CNN architecture where the video is disentangled into spatial and temporal information. By applying the basic depthwise convolution concept to each video frame, spatial information can be efficiently learned; for temporal information, the 3D pointwise convolution can be used to learn the linear combination among sequential frames. To validate and train the proposed model, we collected large quantities of datasets, which are video clips of the physical interactions between two objects under different conditions (illumination and angle variations) and the corresponding interaction forces measured by the haptic sensor (as the ground truth). Our experimental results confirmed our hypothesis; when compared with previous models, the proposed model was more accurate and efficient, and although its model size was 10 times smaller, the 3D convolutional neural network architecture exhibited better accuracy. The experiments demonstrate that the proposed model remains robust under different conditions and can successfully estimate the interaction force between objects.
An update on the safety of treating relapsing-remitting multiple sclerosis

ABSTRACT Introduction: In the last 20 years the armamentarium for multiple sclerosis (MS) treatment has been enriched from an increasingly wider variety of new drugs in order to reach a better control of the disease with a better patient compliance. Areas covered: With this great variety of therapeutic options, physicians may face new and major challenges. The huge amount of data from pilot studies and real-life settings showed that the first-line therapies have a better safety profile. On the other hand, the risks associated with newer drugs, with more selective mechanism of action and targeting specific pathways of MS pathophysiology, are not yet fully established. In particular, real-life use of these advanced drugs has raised important safety issues as long-term effects and potential risks are not yet known and remain to be carefully evaluated. Expert opinion: No time like the present, the physician faces new and major challenges in order to choose the best available therapy for MS. With the increasing number of drugs for treating MS and the lack of safety data, observational studies and post-marketing surveillance activities are crucial in order to improve the knowledge about the safety profile of these drugs and the therapeutic management in clinical practice settings.
Management of Diarrhea in Patients With Carcinoid Syndrome

Abstract Neuroendocrine tumors (NETs) arise from enterochromaffin cells found in neuroendocrine tissues, with most occurring in the gastrointestinal tract. The global incidence of NETs has increased in the past 15 years, likely due to better diagnostic methods. Small-bowel NETs are frequently associated with carcinoid syndrome (CS). Carcinoid syndrome diarrhea occurs in 80% of CS patients and poses a substantial symptomatic and economic burden. Patients with CS diarrhea frequently suffer from diarrhea and flushing and report corresponding impairment in quality of life, requiring substantial changes in daily activities and lifestyle. Treatment paradigms range from surgical debulking to liver-directed therapies to treatment with somatostatin analogs, nonspecific anti-diarrheal agents, and a tryptophan hydroxylase inhibitor. Other causes of diarrhea, including steatorrhea, short bowel syndrome, and bile acid malabsorption, should be considered in NET patients with refractory diarrhea. More therapeutic options are needed for symptomatic management of patients with NETs, and better understanding of the pathophysiology can empower clinicians with improved patient care.
Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes

Significance Covalently linked pili are produced by many gram-positive bacteria; these heteropolymers in various lengths are assembled on the bacterial peptidoglycan by conserved cysteine transpeptidase enzymes named sortases. How the sortase machine controls pilus length and whether pilus length is crucial for bacterial interactions remain elusive. We discover herein that the housekeeping sortase of Actinomyces oris utilizes 2 structural elements for recognition of pilin substrates to control pilus length, hence positioning of pilus tip adhesins. Importantly, we show that optimal positioning of pilus tip adhesins is critical for interspecies interactions, or coaggregation, a prerequisite for the development of oral biofilms. Assembly of pili on the gram-positive bacterial cell wall involves 2 conserved transpeptidase enzymes named sortases: One for polymerization of pilin subunits and another for anchoring pili to peptidoglycan. How this machine controls pilus length and whether pilus length is critical for cell-to-cell interactions remain unknown. We report here in Actinomyces oris, a key colonizer in the development of oral biofilms, that genetic disruption of its housekeeping sortase SrtA generates exceedingly long pili, catalyzed by its pilus-specific sortase SrtC2 that possesses both pilus polymerization and cell wall anchoring functions. Remarkably, the srtA-deficient mutant fails to mediate interspecies interactions, or coaggregation, even though the coaggregation factor CafA is present at the pilus tip. Increasing ectopic expression of srtA in the mutant progressively shortens pilus length and restores coaggregation accordingly, while elevated levels of shaft pilins and SrtC2 produce long pili and block coaggregation by SrtA+ bacteria. With structural studies, we uncovered 2 key structural elements in SrtA that partake in recognition of pilin substrates and regulate pilus length by inducing the capture and transfer of pilus polymers to the cell wall. Evidently, coaggregation requires proper positioning of the tip adhesin CafA via modulation of pilus length by the housekeeping sortase SrtA.
Miniature Untethered EEG Recorder Improves Advanced Neuroscience Methodologies

Rodent electroencephalography (EEG) in preclinical research is frequently conducted in behaving animals. However, the difficulty inherent in identifying EEG epochs associated with a particular behavior or cue is a significant obstacle to more efficient analysis. In this paper we highlight a new solution, using infrared event stamping to accurately synchronize EEG, recorded from superficial sites above the hippocampus and prefrontal cortex, with video motion tracking data in a transgenic Alzheimer's disease (AD) mouse model. Epochs capturing specific behaviors were automatically identified and extracted prior to further analysis. This was achieved by the novel design of an ultra-miniature wearable EEG recorder, the NAT-1 device, and its in-situ IR recording module. The device is described in detail, and its contribution to enabling new neuroscience is demonstrated.
Neurovascular coupling and cerebral autoregulation in atrial fibrillation

The risk of cognitive decline and stroke is increased by atrial fibrillation (AF). We sought to determine whether neurovascular coupling and cerebral autoregulation are blunted in people with AF in comparison with age-matched, patients with hypertension and healthy controls. Neurovascular coupling was assessed using five cycles of visual stimulation for 30 s followed by 30 s with both eyes-closed. Cerebral autoregulation was examined using a sit–stand test, and a repeated squat-to-stand (0.1 Hz) manoeuvre with transfer function analysis of mean arterial pressure (MAP; input) and middle cerebral artery mean blood flow velocity (MCA Vm; output) relationships at 0.1 Hz. Visual stimulation increased posterior cerebral artery conductance, but the magnitude of the response was blunted in patients with AF (18 [8] %; mean [SD]) and hypertension (17 [8] %), in comparison with healthy controls (26 [9] %) (P < 0.05). In contrast, transmission of MAP to MCA Vm was greater in AF patients compared to hypertension and healthy controls, indicating diminished cerebral autoregulation. We have shown for the first time that AF patients have impaired neurovascular coupling responses to visual stimulation and diminished cerebral autoregulation. Such deficits in cerebrovascular regulation may contribute to the increased risk of cerebral dysfunction in people with AF.
Social Support of Male Breast Cancer Patients—a Mixed-Methods Analysis

The aim of this study is to explore the social support of male breast cancer patients (MBCP) in Germany. In particular, three aspects of social support focus on (a) the used resources within the social environment, (b) the received support, and (c) the differences of used social support between MBCP. A mixed-methods design is applied including data of qualitative interviews (N = 27 MBCP) and a written questionnaire (N = 100 MBCP). MBCP use different resources of support from their social environment like partners, family, friends, colleagues, other breast cancer patients, and medical experts. Mostly, MBCP receive emotional and informational support. They often receive emotional support from their partners and informational support from medical experts. Different types of social support usage can be identified dependent on age, occupation, and severity of disease. The older the patients and the less the disease severity, the less social support MBCP use. Within cancer care, partners and the closer social environment should be included more as they are a key resource for MBCP. As health-care professions might also be an important resource of support for MBCP, further research should examine this resource.
Study of the Application of Deep Convolutional Neural Networks (CNNs) in Processing Sensor Data and Biomedical Images

The fast progress in research and development of multifunctional, distributed sensor networks has brought challenges in processing data from a large number of sensors. Using deep learning methods such as convolutional neural networks (CNN), it is possible to build smarter systems to forecasting future situations as well as precisely classify large amounts of data from sensors. Multi-sensor data from atmospheric pollutants measurements that involves five criteria, with the underlying analytic model unknown, need to be categorized, so do the Diabetic Retinopathy (DR) fundus images dataset. In this work, we created automatic classifiers based on a deep convolutional neural network (CNN) with two models, a simpler feedforward model with dual modules and an Inception Resnet v2 model, and various structural tweaks for classifying the data from the two tasks. For segregating multi-sensor data, we trained a deep CNN-based classifier on an image dataset extracted from the data by a novel image generating method. We created two deepened and one reductive feedforward network for DR phase classification. The validation accuracies and visualization results show that increasing deep CNN structure depth or kernels number in convolutional layers will not indefinitely improve the classification quality and that a more sophisticated model does not necessarily achieve higher performance when training datasets are quantitatively limited, while increasing training image resolution can induce higher classification accuracies for trained CNNs. The methodology aims at providing support for devising classification networks powering intelligent sensors.
IFN-λ Decreases Murid Herpesvirus-4 Infection of the Olfactory Epithelium but Fails to Prevent Virus Reactivation in the Vaginal Mucosa

Murid herpesvirus-4 (MuHV-4), a natural gammaherpesvirus of rodents, can infect the mouse through the nasal mucosa, where it targets sustentacular cells and olfactory neurons in the olfactory epithelium before it propagates to myeloid cells and then to B cells in lymphoid tissues. After establishment of latency in B cells, viral reactivation occurs in the genital tract in 80% of female mice, which can lead to spontaneous sexual transmission to co-housed males. Interferon-lambda (IFN-λ) is a key player of the innate immune response at mucosal surfaces and is believed to limit the transmission of numerous viruses by acting on epithelial cells. We used in vivo plasmid-mediated IFN-λ expression to assess whether IFN-λ could prophylactically limit MuHV-4 infection in the olfactory and vaginal mucosae. In vitro, IFN-λ decreased MuHV-4 infection in cells that overexpressed IFN-λ receptor 1 (IFNLR1). In vivo, prophylactic IFN-λ expression decreased infection of the olfactory epithelium but did not prevent virus propagation to downstream organs, such as the spleen where the virus establishes latency. In the olfactory epithelium, sustentacular cells readily responded to IFN-λ. In contrast, olfactory neurons did not respond to IFN-λ, thus, likely allowing viral entry. In the female genital tract, columnar epithelial cells strongly responded to IFN-λ, as did most vaginal epithelial cells, although with some variation from mouse to mouse. IFN-λ expression, however, failed to prevent virus reactivation in the vaginal mucosa. In conclusion, IFN-λ decreased MuHV-4 replication in the upper respiratory epithelium, likely by protecting the sustentacular epithelial cells, but it did not protect olfactory neurons and failed to block virus reactivation in the genital mucosa.
Galactic rotation curves of spiral galaxies and dark matter in f(ℛ,T) gravity theory

Galactic rotation curve is a powerful indicator of the state of the gravitational field within a galaxy. The flatness of these curves indicates the presence of dark matter in galaxies and their clusters. In this paper, we focus on the possibility of explaining the rotation curves of spiral galaxies without postulating the existence of dark matter in the framework of $f(\mathcal{R},T)$ gravity, where the gravitational Lagrangian is written by an arbitrary function of $\mathcal{R}$, the Ricci scalar and of $T$, the trace of energy-momentum tensor $T_{\mu\nu}$. We derive the gravitational field equations in this gravity theory for the static spherically symmetric spacetime and solve the equations for metric coefficients using a specific model that has minimal coupling between matter and geometry. The orbital motion of a massive test particle moving in a stable circular orbit is considered and the behavior of its tangential velocity with the help of the considered model is studied. We compare the theoretical result predicted by the model with observations of a sample of nineteen galaxies by generating and fitting rotation curves for the test particle to check the viability of the model. It is observed that the model could almost successfully explain the galactic dynamics of these galaxies without the need of dark matter at large distances from the galactic center.
Evaluation of pain management in medical transfer of trauma patients by air.

OBJECTIVES With regionalized trauma care, medical transport times can be prolonged, requiring paramedics to manage patient care and symptoms. Our objective was to evaluate pain management during air transport of trauma patients. METHODS We conducted a 12-month review of electronic paramedic records from a provincial critical care transport agency. Patients were included if they were ≥18 years old and underwent air transport to a trauma centre, and excluded if they were Glasgow Coma Scale score <14, intubated, or accompanied by a physician or nurse. Demographics, injury description, and transportation parameters were recorded. Outcomes included pain assessment via 11-point numerical rating scale, patterns of analgesia administration, and analgesia-related adverse events. Results were reported as mean ± standard deviation, [range], (percentage). RESULTS We included 372 patients: 47.0 years old; 262 males; 361 blunt injuries. Transport duration was 82.4 ± 46.3 minutes. In 232 (62.4%) patients who received analgesia, baseline numerical rating scale was 5.9 ± 2.5. Fentanyl was most commonly administered at 44.3 [25-60] mcg. Numerical rating scale after first analgesia dose decreased by 1.1 [-2-7]. Thereafter, 171 (73.7%) patients received 2.4 [1-18] additional doses. While 44 (23.4%) patients had no change in numerical rating scale after first analgesia dose, subsequent doses resulted in no change in numerical rating scale in over 65% of patients. There were 43 adverse events recorded, with nausea the most commonly reported (39.5%). CONCLUSIONS Initial and subsequent dose(s) of analgesic had minimal effect on pain as assessed via numerical rating scale, likely due in part to inadequate dosing. Future research is required to determine and address the barriers to proper analgesia.