Miracle-dz/UniFAR-FTUs huggingface dataset:
MBL2, MASP2, AMELX, and ENAM gene polymorphisms and dental caries in Polish children.

OBJECTIVE The aim of the study was to examine whether the MBL2 C(-290)G and G161A, MASP2 A359G, AMELX C287T and C522T, and ENAM C2452T polymorphisms are associated with dental caries. SUBJECTS AND METHODS Genomic DNA of 95 Polish children with 'higher caries experience' (HC) and 84 subjects with 'lower caries experience' (LC) belonging to two age-groups (5 and 13 years old) was extracted from the buccal mucosa. SNPs were genotyped with PCR-RFLP methods. RESULTS Among 5-year-old children, we found significantly higher percentage of subjects carrying MBL2 (-290)G allele in HC group compared with LC group (43.2%vs 17.6%, P = 0.023). MBL2 C(-290)G-G161A C-G haplotype was overrepresented in LC group in 5-year-olds (P = 0.01), while the opposite association was observed in 13-year-olds, where C-G was overrepresented in HC group (P = 0.028). In 5-year-old children, the frequency of MBL2 G-G haplotype was higher in HC group compared with LC subjects (P = 0.045), while the opposite association (with borderline significance) was observed in 13-year-old children (P = 0.057). SNPs in MASP2, AMELX, and ENAM were not associated with dental caries. CONCLUSION MBL2 gene polymorphism is associated with caries experience in Polish children, but the direction of this association seems to be opposite in primary and permanent dentition.
Viscous drag on the flagellum activates Bacillus subtilis entry into the K‐state

Bacillus subtilis flagella are not only required for locomotion but also act as sensors that monitor environmental changes. Although how the signal transmission takes place is poorly understood, it has been shown that flagella play an important role in surface sensing by transmitting a mechanical signal to control the DegS‐DegU two‐component system. Here we report a role for flagella in the regulation of the K‐state, which enables transformability and antibiotic tolerance (persistence). Mutations impairing flagellar synthesis are inferred to increase DegU‐P, which inhibits the expression of ComK, the master regulator for the K‐state, and reduces transformability. Tellingly, both deletion of the flagellin gene and straight filament (hagA233V) mutations increased DegU phosphorylation despite the fact that both mutants had wild type numbers of basal bodies and the flagellar motors were functional. We propose that higher viscous loads on flagellar motors result in lower DegU‐P levels through an unknown signaling mechanism. This flagellar‐load based mechanism ensures that cells in the motile subpopulation have a tenfold enhanced likelihood of entering the K‐state and taking up DNA from the environment. Further, our results suggest that the developmental states of motility and competence are related and most commonly occur in the same epigenetic cell type.
Identification of Early Predictors for Post-Traumatic Pneumonia

We demonstrated that the standard clinical criteria of fever, leukocytosis, purulent sputum, and infiltrate on chest radiograph are nonspecific for the diagnosis of post-traumatic pneumonia, and only ∼50 per cent of patients with these conditions have pneumonia. Quantitative cultures of bronchoalveolar lavage effluent will differentiate pneumonia (requiring antibiotic therapy) from systemic inflammatory response syndrome (not requiring antibiotics). Early identification of patients at risk for pneumonia can target populations for clinical research. Because risk factors for pneumonia when diagnosed by quantitative cultures have not been defined we reviewed our recent experience to identify variables predictive of pneumonia. Patients over a 22-month period who survived >48 hours were identified from the trauma registry. Pneumonia was defined as growth of ≥105 organisms per milliliter in the bronchoalveolar lavage effluent. Risk factors evaluated included injury severity and severity of shock. There were 7503 patients (75% with blunt and 25% with penetrating injuries). The incidence of pneumonia was 6 per cent (7% of patients with blunt and 2% of patients with penetrating injuries). Logistic regression analysis identified age; Glasgow Coma Scale score; Injury Severity Score; transfusion requirements during resuscitation; spinal cord injury; chest injury severity; and emergent femur fixation, craniotomy, and laparotomy as being independent predictors of pneumonia. We conclude that multiple risk factors, which are all able to be determined early after injury, are predictive of post-traumatic pneumonia. Prompt identification of this high-risk group of patients allows prognostic considerations relative to patient management schemes and targets populations for prophylactic measures or immunomodulation.
Active control system for low-frequency road noise combined with an audio system

An active control system for low-frequency road noise in automobiles combined with an audio system is developed as a commercial application for the first time in the world, and installed in a station wagon. The purpose of this paper is to provide an outline of the system and describe the newly developed cost-reduction technology used for it, since the reduction of system costs is a major reason that active noise control technology could successfully be applied in a commercial product. The methods used to reduce costs include utilization of feedback control, implementation by analogue circuits, and common use of audio system speakers. This system reduces low-frequency road noise in the front seat by about 10 dB and improves audio system listening experience while driving.
Enterprise security pattern: a new type of security pattern

In recent years, most organizations have suffered attacks against their information systems. For this reason, organizations should seek support from enterprise security architectures ESAs in order to secure their information assets. Security patterns can help when building complex ESAs, but they have some limitations that reduce their usability. In this paper, we define the metapattern of a new type of security pattern called Enterprise Security Pattern. This new metapattern provides a model-driven environment and combines all elements that must be considered when designing and building ESAs. We present here a precise meta-model and four diagrams to describe the metapattern of the enterprise security patterns. When avoiding a security problem, organizations could use enterprise security patterns to provide their designers with an optimal and proven security guideline and so standardize the design and building of the ESA for that problem. Enterprise security patterns could also facilitate the selection and tailoring of security policies, patterns, mechanisms, and technologies when a designer is building ESAs. To illustrate our ideas, we present an instance of this new type of pattern, showing how it can be used. Copyright © 2014 John Wiley & Sons, Ltd.
Risk factors for combined hepatocellular-cholangiocarcinoma: a hospital-based case-control study.

AIM To identify risk factors contributing to the development of combined hepatocellular-cholangiocarcinoma (CHC) in China. METHODS One hundred and twenty-six patients with CHC and 4:1 matched healthy controls were interviewed during the period from February 2000 to October 2012. Logistic regression analysis was used to calculate odds ratios (OR) and 95% confidence intervals (CI) for each risk factor. RESULTS Univariate analysis showed that the significant risk factors for CHC development were hepatitis B virus (HBV) infection, heavy alcohol consumption, a family history of liver cancer, and diabetes mellitus. Multivariate stepwise logistic regression analysis showed that HBV infection (OR = 19.245, 95%CI: 13.260-27.931) and heavy alcohol consumption (OR = 2.186, 95%CI: 1.070-4.466) were independent factors contributing to the development of CHC. CONCLUSION HBV infection and heavy alcohol consumption may play a role in the development of CHC in China.
The diversity of allosteric controls at the gateway to aromatic amino acid biosynthesis

Present within bacteria, plants, and some lower eukaryotes 3‐deoxy‐D‐arabino‐heptulosonate 7‐phosphate synthase (DAHPS) catalyzes the first committed step in the synthesis of a number of metabolites, including the three aromatic amino acids phenylalanine, tyrosine, and tryptophan. Catalyzing the first reaction in an important biosynthetic pathway, DAHPS is situated at a critical regulatory checkpoint—at which pathway input can be efficiently modulated to respond to changes in the concentration of pathway outputs. Based on a phylogenetic classification scheme, DAHPSs have been divided into three major subtypes (Iα, Iβ, and II). These subtypes are subjected to an unusually diverse pattern of allosteric regulation, which can be used to further subdivide the enzymes. Crystal structures of most of the regulatory subclasses have been determined. When viewed collectively, these structures illustrate how distinct mechanisms of allostery are applied to a common catalytic scaffold. Here, we review structural revelations regarding DAHPS regulation and make the case that the functional difference between the three major DAHPS subtypes relates to basic distinctions in quaternary structure and mechanism of allostery.
Premeditated Assaults on Young Boys by a Man with Spina Bifida and Hydrocephalus — A Cognitive-Behavioural Approach to Treatment

This paper describes the case of a 25-year-old man with spina bifida and hydrocephalus, who had at least a ten-year history of physically assaulting young boys. Anti-social behaviour is reported as comparatively rare in people with this disability, and the case study illustrates how psychological assessment and treatment techniques were successfully applied. Information from his personal history plus a functional analysis of the assaults suggested that a combination of existing psychological difficulties and learning experiences contributed to the development of his behaviour. A behavioural model of the psychological mechanisms underlying the behaviour was suggested and treatment objectives defined. Cognitive and behavioural treatment techniques were adapted where necessary, with a good outcome. A gap in services for physically handicapped people with behavioural problems is noted and briefly discussed.
The influence of 5-year therapy with tibolone on the lipid profile in postmenopausal women with mild hypercholesterolemia

Our objective was to investigate the effects of 5-year therapy with tibolone on the lipid profile in postmenopausal women with mild hypercholesterolemia (total cholesterol, 241 ± 7 mg/dl; LDL cholesterol, 153 ± 9 mg/dl). Eighty-two patients were divided into two groups. Group A (53 women) received 2.5 mg of tibolone per day. Group B (29 women) received no tibolone. Total, low- and high-density lipoprotein cholesterol and lipoprotein(a) were found to be decreased in the tibolone group, by 17.7%, 32%, 15.5% and 12%, respectively (p < 0.01) throughout the 5-year treatment, while triglycerides showed no significant change. The lipid profile in the control group remained at its initial values. Menopausal symptoms disappeared in the treatment group within the first 5 months, whereas they deteriorated in the control group during the first 2 years. Although a few unwanted side-effects on hormonedependent tissues were observed (including vaginal spotting in 11.3% and febrile hemorrhagic cystic mastopathy in 3.8%) long-term therapy with tibolone seemed to be well tolerated, and appeared to have a beneficial effect on the levels of serum lipids.
Application of sunscreen − theory and reality

We present research on sunscreen use with possible pitfalls and discuss theory vs. reality. A literature review in PubMed was conducted using the terms ‘sunscreen application’, ‘sunscreen use’ and ‘sun protection factor’. The sun protection factor (SPF) of sunscreens are tested using a thickness of 2 mg/cm2, but investigations show that sunscreen under natural conditions is applied insufficiently with amounts about 0.39 to 1.0 mg/cm2, which decreases the protection factor considerably. It has been shown that early reapplication or use of very high SPF (70–100) may partly compensate for the discrepancy between the amounts of sunscreen applied during testing and in reality, and that sunscreen application can be improved by education of consumers. Missing areas and ultraviolet radiation exposure before sunscreen application are other pitfalls that reduce the protective effect of sunscreens considerably. Current sunscreen labelling overrates the protective effect of a given sunscreen when the reality of sunscreen use is taken into account. This may possibly mislead consumers to feel it is safe to extend sun exposure. Alternatively to educating people to use large amounts of sunscreen, we suggest a simple teaching strategy: (1) Apply before sun exposure and (2) Reapply once within 1 h.
Metapopulation Extinction in Fragmented Landscapes: Using Bacteria and Protozoa Communities as Model Ecosystems

Extinction is notoriously difficult to study because of the long timescales involved and the difficulty in ascertaining that extinction has actually occurred. The effect of habitat subdivision, or fragmentation, on extinction risk is even harder to study, as it requires copious replication of habitat patches on large spatial scales and control of area effects between treatments. I used simple small‐scale communities of bacteria and protozoa to study extinction in response to habitat loss and habitat fragmentation. I studied several different community configurations, each with three trophic levels. Unlike most metapopulation studies (experimental as well as theoretical), which have tended to deal with inherently unstable species interactions, I deliberately used community configurations that were persistent in large stock cultures. I recorded the time to extinction of the top predator in single habitat patches of different sizes and in fragmented systems with different degrees of subdivision but the same amount of available habitat. Habitat loss reduced the time to extinction of isolated populations. Fragmented systems went extinct sooner than corresponding unfragmented (continuous) systems of the same overall size. Unfragmented populations persisted longer than fragmented systems (metapopulations) with or without dispersal corridors between subpopulations. In fact, fragmented systems where the fragments were linked by dispersal corridors went extinctly significantly sooner than those where subpopulations were completely isolated from each other. If these results extend to more “natural” systems, it suggests a need for caution in management programs that emphasize widespread establishment of wildlife corridors in fragmented landscapes.
The infancy of the alphabet

Abstract Babylonian and Egyptian writing systems dominated the Near East during the third and second millennia B.C., but during the latter many other scripts arose. Among them was the infant alphabet whose history can be reconstructed in outline with the aid of scattered and brief inscriptions from the Levant. Egyptian influence is seen as a stimulus in its conception and as a factor in the materials and direction of writing. By 1000 B.C. this simple and flexible system was well‐established, supplanting its local rivals, and ready for its expansion eastwards through Aramaic and for its adaptation by the Greeks in the west where it came to its maturity.
FORKING AND DIVIDING IN CONTINUOUS LOGIC

We investigate an open question concerning properties of algebraic independence in continuous theories (see Section 4). The rest of the work is essentially a translation to continuous logic of popular notions of independence (in particular, forking and dividing). Much of the time we are simply “copying” classical proofs from well-known sources, while along the way making the necessary adjustments for continuous languages.
The Relationship Between Serum TARC, TSLP and POSTN Levels and Childhood Atopic Dermatitis.

BACKGROUND The aim of the study is to evaluate the relationship between serum periostin (POSTN), thymus and activation-regulated chemokine (TARC), and thymic stromal lymphopoietin (TSLP) levels and disease severity and atopy in children with atopic dermatitis. METHODS Sixty children with atopic dermatitis and 31 healthy controls were included in the study. The disease severity was measured by SCORAD scores. Serum POSTN, TARC, and TSLP levels were measured in all participants. The demographic data were recorded, and skin prick tests were performed to evaluate atopy in children with atopic dermatitis. RESULTS Serum POSTN, TARC, and TSLP levels were higher in children with atopic dermatitis than in healthy children (p = 0.041, p = 0.034, and p < 0.001, respectively). Serum POSTN levels were higher with atopy than without atopy in children with atopic dermatitis (p = 0.047). There was a positive moderate correlation between POSTN and the age and symptom duration in children with atopic dermatitis (r = 0.343, p = 0.007, r = 0.484, and p < 0.0001, respectively). In receiver operating characteristics (ROC) analysis, the area under the curve (AUC) was 0.789 (95% CI (0.694 - 0.883), p < 0.0001) for TSLP and the AUC was 0.636 (95% CI (0.522 - 0.750), p = 0.034) for TARC to predict severe atopic dermatitis. CONCLUSIONS Serum POSTN, TARC, and TSLP were higher in children with atopic dermatitis. Serum TARC and TSLP levels might be used as biomarkers to predict severe atopic dermatitis and serum POSTN to predict atopy and disease chronicity.
Lack of objective response of myelodysplastic syndromes and acute myeloid leukemia to decitabine after failure of azacitidine

Abstract The hypomethylating agents azacitidine and decitabine are standard therapy for myelodysplastic syndromes (MDS), and are often used to treat patients with acute myeloid leukemia (AML) unlikely to benefit from cytotoxic chemotherapy. Switching hypomethylating agents after treatment failure is common, but this approach is not well studied. We retrospectively reviewed data on 25 patients with MDS, MDS/myeloproliferative neoplasm (MDS/MPN) or AML who were treated with decitabine after primary or secondary azacitidine failure at the University of Maryland Greenebaum Cancer Center. Five patients with MDS or MDS/MPN achieved stable disease with decitabine, but no patient achieved complete or partial remission or hematologic improvement. Most patients discontinued therapy due to disease progression or death after a median of 2 cycles and median survival was 5.9 months after decitabine initiation. Based on our data, decitabine therapy after azacitidine failure is of little benefit beyond disease stabilization in some patients.
Carboxymethylation of Anacardium Occidentale L . Exudate Gum : Synthesis and Characterization

The use of natural gums taken from the exudates and extracts of plants has been given a strong impulse due to the lucrative possibilities for industrialization and excellent international market. Anacardium occidentale L gum consists of highly branched galactan framework comprising of chains of (1-3) – linked with β-D-galacto pyranosyl units interspersed with β-(1-6)-linkages, possessing hydroxyl groups available for the attachment of biologically active compounds. The modification discussed includes the introduction of carboxymethyl groups into the molecules for extending the applications of partially carboxymethylated A. occidentale gum. The carboxymethylation conditions with respect to the volume and concentration of sodium hydroxide, weight of chloro-acetic acid in a reaction temperature were optimized. The solubility of modified gum was higher than the unmodified gum. The resulting product was characterized by FTIR spectroscopy. The carboxymethylated gum may provide an efficient alternative approach for the oral delivery of hydrophilic macromolecules.
The Role of Maternal HIV Envelope-Specific Antibodies and Mother-to-Child Transmission Risk

Despite the wide availability of antiretroviral therapy (ART) prophylaxis during pregnancy, >150,000 infants become infected through mother-to-child transmission (MTCT) of HIV worldwide. It is likely that additional intervention strategies, such as a maternal HIV vaccine, will be required to eliminate pediatric HIV infections. A deeper understanding of the fine specificity and function of maternal HIV envelope (Env)-specific responses that provide partial protection against MTCT will be critical to inform the design of immunologic strategies to curb the pediatric HIV epidemic. Recent studies have underlined a role of maternal HIV Env-specific neutralizing and non-neutralizing responses in reducing risk of MTCT of HIV and in prolonging survival rates in HIV-infected infants. However, critical gaps in our knowledge include (A) the specific role of maternal autologous-virus IgG-neutralizing responses in driving the selection of infant transmitted founder (T/F) viruses and (B) Env mechanisms of escape from maternal autologous virus-neutralizing antibodies (NAbs). A more refined understanding of the fine specificities of maternal autologous virus NAbs and ways that maternal circulating viruses escape from these antibodies will be crucial to inform maternal vaccination strategies that can block MTCT to help achieve an HIV-free generation.
Dosage des HAP dans les produits de pêche par GC-MS au niveau des côtes marocaines

Les HAP etant largement repandus et fortement susceptibles d’entrainer des effets biologiques nefastes, ils ont fait l’objet de plusieurs etudes et evaluations. Les Hydrocarbure Aromatique Polycyclique (HAP) sont une serie d'hydrocarbures dont les atomes de carbone sont disposes en cycles benzeniques unis les uns aux autres. Ils presentent un risque toxicologique important meme a de faibles concentrations, lies surtout a leurs proprietes cancerigenes et/ou mutagenes. De plus, les HAP font partie des contaminants peu biodegradables ce qui les rend persistants dans les milieux naturels. Ainsi, leur sort dans l'environnement est devenu un sujet preoccupant. Pour l'homme, la source majoritaire d'exposition aux HAP pour les non fumeurs est l'ingestion des denrees alimentaires (viandes, poissons, legumes, etc.). Cette etude est menee sur les produits de peche preleves le long du littoral marocain, debarques au niveau des principaux ports du royaume. Les analyses des teneurs moyennes des HAP dans les produits locaux variaient significativement selon les sites (p<0,05). Les resultats montrent une qualite satisfaisante de ces produits pour la consommation. Les sites des prelevements sont choisis de maniere a diagnostiquer l’etat de la contamination organique dans les differents produits peches. Le dosage de ces polluants est realise par chromatographie gazeuse couplee a la spectrometrie de masse.