Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of herpes simplex virus 1 alpha proteins 0, 4, and 27 with monoclonal antibodies

Analyses of the reactivity and patterns of synthesis of infected cell polypeptides (ICPs) specified by herpes simplex virus (HSV) 1 and 2 and by HSV-1 X HSV-2 recombinants indicated that monoclonal antibody H1183 reacted with HSV-1 alpha ICP0, whereas monoclonal antibody H1113 reacted with both HSV-1 and HSV-2 alpha ICP27. H1083 and H1113 and a monoclonal antibody to ICP4 (H640) similar to one previously described (D. K. Braun et al., J. Virol. 46:103-112.) were then used to study the properties of these alpha proteins. The results were as follows: alpha ICP0, ICP4, and ICP27 accumulated primarily in the nuclei of infected cells. ICP4 and ICP27 were poorly soluble in nondenaturing buffer solutions. ICP0 was considerably more soluble than ICP4 and ICP27. ICP0, ICP4, and ICP27 were readily partially purified by immunoaffinity chromatography from lysates of infected cells solubilized with denaturing agents such as sodium dodecyl sulfate. ICP0 and ICP27 were phosphorylated in cells overlaid with medium containing 32P early (1 to 3 h) or late (18 to 20 h) postinfection. A fraction, but not all, 32P that was incorporated early was chased in the presence of unlabeled phosphate. ICP0, ICP4, and ICP27 labeled with either 32P or [35S]methionine yielded multiple spots upon two-dimensional separations. However, ICP4 quantitatively precipitated at the origin when the migration in the first dimension was from acid to base, and both ICP4 and ICP27 partially precipitated at the origin when the direction of migration was reversed.
Extracting cyber threat intelligence from hacker forums: Support vector machines versus convolutional neural networks

Hacker forums and other social platforms may contain vital information about cyber security threats. But using manual analysis to extract relevant threat information from these sources is a time consuming and error-prone process that requires a significant allocation of resources. In this paper, we explore the potential of Machine Learning methods to rapidly sift through hacker forums for relevant threat intelligence. Utilizing text data from a real hacker forum, we compared the text classification performance of Convolutional Neural Network methods against more traditional Machine Learning approaches. We found that traditional machine learning methods, such as Support Vector Machines, can yield high levels of performance that are on par with Convolutional Neural Network algorithms.
A longitudinal investigation of cognitive function in children and adolescents with type 1 diabetes mellitus

Cross‐sectional studies find altered cognition in youth with type 1 diabetes mellitus (T1DM). However, few longitudinal studies have examined the trajectories of their cognitive performance over time. The aims of this study were to explore longitudinal change in cognitive function in youth with T1DM as compared with nondiabetic sibling controls, and how glycemic control and age of onset influence cognitive performance over time.
Neurilemoma About the Knee

Neurilemoma, although relatively rare, is the most commonly occurring peripheral nerve sheath tumor. The term is often used interchangeably with schwannoma, although “neurilemoma” is currently preferred. Neurilemoma symptoms are often present for a significant length of time before a diagnosis is made because the clinical appearance is often vague and confusing. Therefore, neurilemoma should be considered in the differential diagnosis of a patient complaining of a painful soft tissue mass or nerve compression symptoms.
ATP‐binding cassette transporter hallmarks tissue macrophages and modulates cytokine‐triggered polarization programs

Macrophages are central players in both lipid metabolism and innate immunity. Their determinant role in the pathogenesis of atherosclerosis is under the control of the ATP‐binding cassette transporter (ABCA1), which by minimizing cellular lipid content, limits development of pro‐inflammatory foam cells. Considering the differential contribution of monocyte subsets to the generation of vascular lesions we analyzed the immunophenotype of ABCA1‐expressing cells in the myeloid lineage, by the combined use of flow cytometry and real‐time quantitative RT‐PCR. ABCA1 expression is limited to “non‐inflammatory” Ly6Clo circulating monocytes and tissue‐resident macrophages expressing markers of alternative activation. In ABCA1−/− peritoneal macrophages the transcriptional programs induced by LPS/IFN‐γ or IL‐4 cytokines are altered and deviated phosphorylation patterns of STAT transcriptional regulators in response to stimuli are observed.
Microdomain Orientation of PS-b-PMMA by Controlled Interfacial Interactions

The microdomain orientation in thin films of cylinder- and lamella-forming diblock copolymers, polystyrene-block-poly(methyl methacrylate) (PS-b-PMMA), was investigated as a function of the film thickness and the composition of random copolymers composed of styrene (S) and methyl methacrylate (MMA), denoted as P(S-r-MMA), that were anchored to the substrate. Using scanning force microscopy (SFM) and grazing incidence small-angle X-ray scattering (GISAXS), the dependence of the microdomain orientation on film thickness around lattice period (or d-spacing, L0), where the microdomain orientations normal to the film surface could be achieved, showed that the optimal condition for the balanced interfacial interactions (the so-called neutrality in random copolymer) was 0.64 of PS mole fraction (XPS) for the cylindrical microdomain having narrow compositional range of P(S-r-MMA) from 0.52 to 0.72 of XPS, whereas for the lamella microdomain it was observed at XPS = 0.55 ranging extensively from 0.48 to 0.78.
Axiverse cosmology and the energy scale of inflation

Ultralight axions (${m}_{a}\ensuremath{\lesssim}{10}^{\ensuremath{-}18}\text{ }\text{ }\mathrm{eV}$), motivated by string theory, can be a powerful probe of the energy scale of inflation. Here it is shown that in contrast to heavier axions the isocurvature modes in the ultralight axions can coexist with observable gravitational waves. Large-scale structure constraints severely limit the parameter space for axion mass, density fraction, and isocurvature amplitude. It is also shown that radically different cosmic microwave background observables for the ultralight axion isocurvature mode additionally reduce this space. The results of a new, accurate, and efficient method to calculate this isocurvature power spectrum are presented and can be used to constrain ultralight axions and inflation.
Direction-specific adaptation effects acquired in a slow rotation room.

Abstract : Thirty-eight subjects were required to execute 120 head movements in a slow rotation room at each 1-rpm increase in velocity of the room between 0 and 6 rpm and, after a single-step gradual return to zero velocity, execute 120 head movements either immediately after the return or after delay periods varying from 1 to 24 hours unless, at any time, more than mild symptoms of motion sickness were elicited. A second stress profile differed by the sequential addition of an incremental adaptation schedule (identical to the first) in which the direction of rotation was reversed. The experimental findings demonstrated the acquisition of direction-specific adaptation effects that underwent spontaneous decay with a short time constant (hours). With their disappearance a nondirection-specific adaptation was revealed with a long time constant (days). Speculations are presented which could account for the simultaneous acquisition of short-term and long-term adaptation effects. The findings support the theory that motion sickness, although a consequence of vestibular stimulation, has its immediate origin in nonvestibular systems, implying a 'facultative' or temporary linkage between the vestibular and nonvestibular systems. (Author)
Affordances and Constraints of Intelligent Decision Support for Military Command and Control : Three Case Studies of Support Systems ; Möjligheter och begränsningar med intelligent beslutsstöd i militär ledning : Tre fallstudier av teknikstöd

We present results from a study on constructing and evaluating a support tool for the extraction of patterns in distributed decision -making processes, based on design criteria elicited from a study on the work process involved in studying such decision-making. Specifically, we devised and evaluated an analysis tool for C2 researchers who study simulated decision-making scenarios for command teams. The analysis tool used text clustering as an underlying pattern extraction technique and was evaluated together with C2 researchers in a workshop to establish whether the design criteria were valid and the approach taken with the analysis tool was sound. Design criteria elicited from an earlier study with researchers (open-endedness and transparency) were highly consistent with the results from the workshop. Specifically, evaluation results indicate that successful deployment of advanced analysis tools requires that tools can treat multiple data sources and offer rich opportunities for manipulation and interaction (open-endedness) and careful design of visual presentations and explanations of the techniques used (transparency). Finally, the results point to the high relevance and promise of using text clustering as a support for analysis of C2 data.
New data on the morphology of Comephoronema oschmarini (Nematoda, Cystidicolidae), a little-known gastrointestinal parasite of Lota lota (Teleostei) in Palaearctic Eurasia

The nematode Comephoronema oschmarini Trofimenko, 1974 (Cystidicolidae) was found in the stomach of the burbot Lota lota (Linnaeus) of Lake Geneva, Savoy, France, collected on 23 March 2000 [prevalence 60% (3/5); intensity of infection 2–66 (mean 35)]. This material made it possible to study in detail the morphology of this so far little-known parasite, using both light and scanning electron microscopy (the latter not previously used for this species). The SEM examination showed taxonomically important morphological features, not previously reported, including the presence of distinct terminal pseudolabial projections (protuberances), four submedian labia, four bilobed submedian sublabia, and only four cephalic papillae. Deirids were found to be bifurcated. A description of the true arrangement of the two last pairs of minute caudal papillae and phasmids in the male is given. Species of Compehoronema Layman, 1933 are considered to be closely related to those of Ascarophis van Beneden, 1871. A key to valid species of Comephoronema is provided.
A PolSAR ship detector using full combination of polarimetric channel

Polarimetric Synthetic Aperture Radar (PolSAR) is an advanced sensor which can achieve echoes from the ship in four different polarization channels. Given a proper weighting vector of each channel in a combination process, the contrast between ships and background can be enhanced which is a huge benefit. In this paper, a new ship detector is proposed based on an automatic way to evaluate the right weighting vector. In order to speed up the algorithm, a simulated annealing particle swarm optimization (SA-PSO) is used to search the optimal solution in the complex space. Finally, a Fine Quad Radarsat-2 image is used between PWF and the proposed method, which proves the effectiveness and availability of the proposed method.
Connecting common genetic polymorphisms to protein function: A modular project sequence for lecture or lab

Single nucleotide polymorphisms (SNPs) in DNA can result in phenotypes where the biochemical basis may not be clear due to the lack of protein structures. With the growing number of modeling and simulation software available on the internet, students can now participate in determining how small changes in genetic information impact cellular protein structure and function. We have developed a modular series of activities to engage lab or lecture students in examining the basis for common phenotypes. The activities range from basic phenotype testing/observation to DNA sequencing and simulation of protein structure and dynamics. We provide as an example study of the bitterness receptor TAS2R38 and PTC tasting, however these activities are applicable to other SNPs or genomic variants with a direct connection to an observable phenotype. These activities are modular and can be mixed to meet the student capabilities and infrastructure availability. The complete sequence of activities will demonstrate the direct connection between gene structure, protein structure and organism function. © 2016 by The International Union of Biochemistry and Molecular Biology, 44(6):526–536, 2016.
[Advanced oxidation protein products in pregnancy].

OBJECTIVE Pregnancy and mainly its complications are associated with increased oxidative stress. Advanced oxidation protein products (AOPP) can serve as one of its markers. SETTING First Institute of Medical Chemistry and Biochemistry and Institute for Clinical Biochemistry, First Medical Faculty, Charles University; Institute for Care of Mother and Child, Prague. METHODS Together with parameters of prenatal screening, AOPP were measured in the serum of 23 pregnant women in the 2nd trimester of pregnancy. A group of healthy blood donors--women and men was used for comparison. AOPP were determined spectrophotometrically according to Witko-Sarsat (absorbance at 340 nm) and are expressed in chloramin units (mumol/l). RESULTS Serum AOPP concentrations in pregnant women are significantly higher in comparison with blood donors--women (85.90 +/- 18.70 mumol/l vs 57.34 +/- 16.31 mumol/l, P < 0.0001) but there is no statistically significant difference between pregnant women and blood donors--men (85.90 +/- 18.70 mumol/l vs 78.60 +/- 44.01 mumol/l). AOPP level does not correlate either with the age of pregnant women or with the parameters of prenatal screening (human chorionic gonadotrophin--HCG, alpha-1-fetoprotein--AFP and trophoblast-specific--beta-1-glycoproteion--SP1). CONCLUSION AOPP as a marker of oxidative stress is increased in the serum of pregnant women in comparison with women--blood donors but is similar as in men--blood donors which supports the hypothesis of hormonal influence. Nevertheless, AOPP do not correlate with the parameters of prenatal screening (HCG, AFP and SP1).
A longitudinal study of cycloplegic refraction in a cohort of 350 Japanese schoolchildren. Anisometropia

The intereye difference of cycloplegic refraction was determined in a cohort of 350 rural Japanese schoolchildren aged 6 to 11 years. The intereye spherical difference remained statistically unchanged throughout 5 years, with mean value ranging from 0.21 to 0.28 D, median from 0.15 to 0.20 D, and the 95th percentile from 0.58 to 0.85 D. The prevalence of 1.0 D or greater spherical anisometropia was up to 3.1%. Linear regression analyses of the longitudinal data from individual children revealed that 295 (84.3%) children had remained unchanged while 55 (15.7%) showed significant increase or decrease in the amount of anisometropia with increasing age. The intereye astigmatic difference was also statistically unchangeable, with mean value ranging from 0.28 to 0.36 D, median from 0.20 to 0.39 D, and the 95th percentile from 0.70 to 0.90 D. The prevalence of 1.0 D or greater astigmatic anisometropia was up to 4.3%. There was a significant correlation between the spherical and astigmatic anisometropia. The results show the current state of intereye refractive difference in Japanese schoolchildren, demonstrating that it remains unchanged and significant anisometropia is rare during school age.
Triple-Band Unidirectional Circularly Polarized Hexagonal Slot Antenna With Multiple L-Shaped Slits

The design of a triple-band circularly polarized hexagonal slot antenna with L-shaped slits is presented in this study. By adding three L-shaped slit arms to the hexagonal slot, circularly polarized radiation is achieved at three different frequencies. The proposed slot antenna is excited by a simple tapered microstrip feeding line. A conducting reflector is also used to enhance the antenna gain. The measured -10 dB reflection bandwidths are 33.16% (3.22-4.5 GHz) and 22.72% (4.76-5.98 GHz). The measured 3 dB axial ratio bandwidths for the triple bands are 1.7%, 3.86%, and 5.23%, respectively. The measured peak gains within the 3 dB axial ratio bands are 5.5 dBic, 4.63 dBic, and 6.77 dBic.
Intractability of Time-Optimal Multirobot Path Planning on 2D Grid Graphs with Holes

The most tight intractability results for graph-based Multirobot Path Planning (MPP), proven recently, state that time-optimal and distance-optimal MPP problems are NP-hard on planar graphs. In this letter, we go one step further for what concerns the time-optimal objectives, and prove that such problems remain NP-hard when restricting the planar graph to a 2D grid graph with holes, which is a discretization widely used in robotics. Our reduction (from the Boolean satisfiability problem) cannot be easily modified for the distance-optimal objectives, whose hardness remains an open problem.
Characterization of a varicella-zoster virus variant with altered thymidine kinase activity

A varicella-zoster virus (VZV) strain resistant to 5-iodo-2'-deoxyuridine (IdUrd) and 5-bromo-2'-deoxyuridine (BrdUrd) but sensitive to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdUrd) and (E)-5-(2-iodovinyl)-2'-deoxyuridine (IVdUrd) was isolated. The 2'-deoxythymidine (dThd) kinase of this mutant (Ito) strain was characterized; it was much less efficient in phosphorylating dThd, 2'-deoxycytidine, and BrdUrd than were the dThd kinases from wild-type (CaQu, Kobayashi) VZV strains. The Ito dThd kinase had a markedly decreased affinity for dThd, 2'-deoxycytidine, and BrdUrd but only a slightly decreased affinity for IVdUrd than had the wild-type VZV dThd kinase. BrdUrd was incorporated to a much lesser extent in VZV (Ito strain)-infected cells than wild-type VZV-infected cells, but IVdUrd was incorporated in Ito VZV-infected cells as efficiently as in wild-type VZV-infected cells. While resistant to IdUrd and BrdUrd, the Ito strain was susceptible to inhibitors of de novo thymidylate biosynthesis such as aminopterin.
Protein engineering of Saccharomyces cerevisiae transporter Pdr5p identifies key residues that impact Fusarium mycotoxin export and resistance to inhibition

Cereal infection by the broad host range fungal pathogen Fusarium graminearum is a significant global agricultural and food safety issue due to the deposition of mycotoxins within infected grains. Methods to study the intracellular effects of mycotoxins often use the baker's yeast model system (Saccharomyces cerevisiae); however, this organism has an efficient drug export network known as the pleiotropic drug resistance (PDR) network, which consists of a family of multidrug exporters. This study describes the first study that has evaluated the potential involvement of all known or putative ATP‐binding cassette (ABC) transporters from the PDR network in exporting the F. graminearum trichothecene mycotoxins deoxynivalenol (DON) and 15‐acetyl‐deoxynivalenol (15A‐DON) from living yeast cells. We found that Pdr5p appears to be the only transporter from the PDR network capable of exporting these mycotoxins. We engineered mutants of Pdr5p at two sites previously identified as important in determining substrate specificity and inhibitor susceptibility. These results indicate that it is possible to alter inhibitor insensitivity while maintaining the ability of Pdr5p to export the mycotoxins DON and 15A‐DON, which may enable the development of resistance strategies to generate more Fusarium‐tolerant crop plants.
Time to SARS-CoV-2 Clearance Among Patients with Cancer and COVID-19

Question: What is the median time to clearance of SARS-CoV-2 among cancer patients according to currently used criteria? Findings: In this single-institution retrospective cohort study, the median time to SARS-CoV-2 clearance was 50 days using the ASCO/CDC criteria of 2 negative RT-PCR assays >24 hours apart. Using alternative criteria of 1 negative RT-PCR assay (UK-NICE) or CDC clinical criteria (10 days after first positive RT-PCR and 3 days after last symptoms), median clearance times were 31 days and 13 days, respectively. Meaning: SARS-CoV-2 clearance times differ substantially depending on criteria used and may be prolonged in cancer patients.
Positive Mental Health, Subjective Vitality and Satisfaction with Life for French Physical Education Students

The multidimensional model of mental health includes both emotional and functional well-being. Functional well being is made up of psychological and social well-being. The factor structure of the Mental Health Continuum Short Form (MHC-SF), designed to measure these three factors, has been confirmed with a sample of U.S. adolescents and adults. Mental health can be seen as a continuum, where an individual' s mental health may have many different possible values. Mental illness and mental well-being form two continua in the population. The purposes of this study were: 1) To test the factor structure and internal consistency of 5 scales: 4 subjective well-being scales and psychological distress scale (GHQ-12) with 509 French physical education students. 2) Examine the bi-dimensionality of complet mental health by the Confirmatory Factor Analysis (CFA). 3) Testing the correlation among mental well-being, mental illness, subjective vitality and the satisfaction with life. Results show a good structure validity for only 4 scales; the bi-dimensionnality of the complet mental health proposed by Keyes (1). In addition, findings confirm a positive and siignificant correlations among the positive well-being scales (MHC-SF; SVS and SWLS) and negative correlation between these positive constructs and the GHQ-12.
A CBIR System for Hyperspectral Remote Sensing Images Using Endmember Extraction

With the rapid development of remote sensing technology, searching the similar image is a challenge for hyperspectral remote sensing image processing. Meanwhile, the dramatic growth in the amount of hyperspectral remote sensing data has stimulated considerable research on content-based image retrieval (CBIR) in the field of remote sensing technology. Although many CBIR systems have been developed, few studies focused on the hyperspectral remote sensing images. A CBIR system for hyperspectral remote sensing image using endmember extraction is proposed in this paper. The main contributions of our method are that: (1) the endmembers as the spectral features are extracted from hyperspectral remote sensing image by improved automatic pixel purity index (APPI) algorithm; (2) the spectral information divergence and spectral angle match (SID–SAM) mixed measure method is utilized as a similarity measurement between hyperspectral remote sensing images. At last, the images are ranked with descending and the top-M retrieved images are returned. The experimental results on NASA datasets show that our system can yield a superior performance.
Phenytoin has little effect on in‐vitro models of wound healing

Summary It has been reported that phenytoin induces gingival and connective tissue hyperplasia and may be of use in wound healing. In this study the effect of phenytoin on human epidermal keratinocytes and skin fibroblasts has been investigated in vitro. Cell cultures were exposed to increasing concentrations of phenytoin from 10−9 to 10−4 M in the presence of 1 and 10% serum supplemented medium. In addition the effect of phenytoin on epidermal cell migration (epiboly) has been investigated using organ culture of human skin. No stimulation of cell growth was observed, and only a mild toxicity affecting keratinocytes was seen at the highest concentrations. Similarly, no effect on epidermal cell migration in vitro was observed. The lack of a direct effect in vitro suggests that any in‐vivo effect was not the result of interaction between phenytoin and keratinocytes or fibroblasts but possibly due to indirect modulation via other cell types, such as inflammatory or lymphoreticular cells.