Miracle-dz/UniFAR-FTUs huggingface dataset:
Survival of Murine Norovirus, Tulane Virus, and Hepatitis A Virus on Alfalfa Seeds and Sprouts during Storage and Germination

ABSTRACT Human norovirus (huNoV) and hepatitis A virus (HAV) have been involved in several produce-associated outbreaks and identified as major food-borne viral etiologies. In this study, the survival of huNoV surrogates (murine norovirus [MNV] and Tulane virus [TV]) and HAV was investigated on alfalfa seeds during storage and postgermination. Alfalfa seeds were inoculated with MNV, TV, or HAV with titers of 6.46 ± 0.06 log PFU/g, 3.87 ± 0.38 log PFU/g, or 7.01 ± 0.07 log 50% tissue culture infectious doses (TCID50)/g, respectively. Inoculated seeds were stored for up to 50 days at 22°C and sampled during that storage period on days 0, 2, 5, 10, and 15. Following storage, virus presence was monitored over a 1-week germination period. Viruses remained infectious after 50 days, with titers of 1.61 ± 0.19 log PFU/g, 0.85 ± 0.21 log PFU/g, and 3.43 ± 0.21 log TCID50/g for MNV, TV, and HAV, respectively. HAV demonstrated greater persistence than MNV and TV, without a statistically significant reduction over 20 days (<1 log TCID50/g); however, relatively high levels of genomic copies of all viruses persisted over the testing time period. Low titers of viruses were found on sprouts and were located in all tissues as well as in sprout-spent water sampled on days 1, 3, and 6 following seed planting. Results revealed the persistence of viruses in seeds for a prolonged period of time, and perhaps of greater importance these data suggest the ease of which virus may transfer from seeds to sprouts and spent water during germination. These findings highlight the importance of sanitation and prevention procedures before and during germination.
Comparative Characterization of the Microbial Diversities of an Artificial Microbialite Model and a Natural Stromatolite

ABSTRACT Microbialites are organosedimentary structures that result from the trapping, binding, and lithification of sediments by microbial mat communities. In this study we developed a model artificial microbialite system derived from natural stromatolites, a type of microbialite, collected from Exuma Sound, Bahamas. We demonstrated that the morphology of the artificial microbialite was consistent with that of the natural system in that there was a multilayer community with a pronounced biofilm on the surface, a concentrated layer of filamentous cyanobacteria in the top 5 mm, and a lithified layer of fused oolitic sand grains in the subsurface. The fused grain layer was comprised predominantly of the calcium carbonate polymorph aragonite, which corresponded to the composition of the Bahamian stromatolites. The microbial diversity of the artificial microbialites and that of natural stromatolites were also compared using automated ribosomal intergenic spacer analysis (ARISA) and 16S rRNA gene sequencing. The ARISA profiling indicated that the Shannon indices of the two communities were comparable and that the overall diversity was not significantly lower in the artificial microbialite model. Bacterial clone libraries generated from each of the three artificial microbialite layers and natural stromatolites indicated that the cyanobacterial and crust layers most closely resembled the ecotypes detected in the natural stromatolites and were dominated by Proteobacteria and Cyanobacteria. We propose that such model artificial microbialites can serve as experimental analogues for natural stromatolites.
Rapid Growth of Planktonic Vibrio cholerae Non-O1/Non-O139 Strains in a Large Alkaline Lake in Austria: Dependence on Temperature and Dissolved Organic Carbon Quality

ABSTRACT Vibrio cholerae non-O1/non-O139 strains have caused several cases of ear, wound, and blood infections, including one lethal case of septicemia in Austria, during recent years. All of these cases had a history of local recreational activities in the large eastern Austrian lake Neusiedler See. Thus, a monitoring program was started to investigate the prevalence of V. cholerae strains in the lake over several years. Genetic analyses of isolated strains revealed the presence of a variety of pathogenic genes, but in no case did we detect the cholera toxin gene or the toxin-coregulated pilus gene, both of which are prerequisites for the pathogen to be able to cause cholera. In addition, experiments were performed to elucidate the preferred ecological niche of this pathogen. As size filtration experiments indicated and laboratory microcosms showed, endemic V. cholerae could rapidly grow in a free-living state in natural lake water at growth rates similar to those of the bulk natural bacterial population. Temperature and the quality of dissolved organic carbon had a highly significant influence on V. cholerae growth. Specific growth rates, growth yield, and enzyme activity decreased markedly with increasing concentrations of high-molecular-weight substances, indicating that the humic substances originating from the extensive reed belt in the lake can inhibit V. cholerae growth.
An embedded-code multirate speech transform coder

In embedded multirate speech coding, it is desired to have a transmitter/receiver system that operates efficiently over a wide range of channel transmission rates. We are currently investigating a system based on adaptive transform coding of speech, in which we code and transmit the system parameters and the discrete cosine transform (DCT) coefficients of the fullband linear prediction residual waveform. The multirate property of the system is achieved by allowing the channel to discard some of the bits generated by the high data rate transmitter. Stripping off bits results in an absence of DCT components, which the receiver regenerates by a spectral duplication method. An inverse DCT at the receiver yields the time domain residual waveform to be used as input to the linear prediction synthesis filter. The lowest data rate achievable by the system is about 2.5 kb/s, in which case the system reduces to a narrowband LPC pitch-excited vocoder.
In vivo anti-malarial activity of hydroalcoholic extracts from Asparagus africanus Lam. in mice infected with Plasmodium berghei

Background : Malaria is a major public health problem in the world in general and developing countries in particular, causing an estimated 1-2 million deaths per year, an annual incidence of 300-500 million clinical cases and more than 2 billion people are at risk of infection from it. But it is also becoming more difficult to treat malaria due to the increasing drug resistance. Therefore, the need for alternative drugs is acute. Objective : The This study aims at investigating the in vivo antiplasmodial activity of extracts of the roots and area parts from traditionally used medicinal plant, named Asparagus africanus (Liliaceae). Methods : A rodent malaria parasite, Plasmodium berghei , which was maintained at the Ethiopian Health and Nutrition Research Institute (EHNRI) laboratory, was inoculated into Swiss albino mice. The mice were infected with 1x107 parasites intraperitoneally. The extracts were administered by an intra gastric tube daily for four days starting from the day of parasite inoculation. The control groups received the same amount of solvent (vehicle) used to suspend each dose of the herbal drug. Chloroquine was used as a standard drug, and was administered through the same route. Results : Extracts from the roots and aerial parts of A.africanus were observed to inhibit Plasmodium berghei parasitaemia in the Swiss albino mice by 46.1% and 40.7% respectively. Conclusion : The study could partly confirm the claim in Ethiopian traditional medicine that the plant has therapeutic values in human malaria. There is, thus, the need to initiate further in-depth investigation by using different experimental models. The Ethiopian Journal of Health Development Vol. 20 (2) 2006: 112-118
Source location from time differences of arrival: Identifiability and estimation

A new framework is presented for the problem of estimating source location from a set of time difference of arrival (TDOA) measurements. First the conditions under which the source coordinates are identifiable are derived and geometrically interpreted. Then it is shown that the source location can be found, when it is identifiable, by estimating the coefficients of a set of linear relationships, with the number of equations being equal to the number of stations.
Psychometric properties of the Arabic Family Support Scale for families of children and youth with cerebral palsy in Jordan†

Abstract Background Knowledge about family support systems available for Jordanian families of children with cerebral palsy (CP) is limited due to the lack of culturally valid measures of family support. The psychometric properties of the Arabic version of the Family Support Scale (A-FSS) were examined. Methods Parents of 115 children with CP who were receiving services at different settings in Jordan were interviewed using the A-FSS. Results Spouse and professional helpers were rated as the most helpful sources of support, whereas early childhood intervention programs and social groups were the least helpful. A valid structure of the A-FSS was presented for Jordanian families of children with CP with an acceptable level of internal consistency. Conclusions The A-FSS is a valid and reliable measure of family support for Jordanian families of children with CP. Service providers are encouraged to use A-FSS with families to explore options for using sources of support to cope with their children's disability.
Characterization of an Endo-Processive-Type Xyloglucanase Having a β-1,4-Glucan-Binding Module and an Endo-Type Xyloglucanase from Streptomyces avermitilis

ABSTRACT We cloned two glycoside hydrolase family 74 genes, the sav_1856 gene and the sav_2574 gene, from Streptomyces avermitilis NBRC14893 and characterized the resultant recombinant proteins. The sav_1856 gene product (SaGH74A) consisted of a catalytic domain and a family 2 carbohydrate-binding module at the C terminus, while the sav_2574 gene product (SaGH74B) consisted of only a catalytic domain. SaGH74A and SaGH74B were expressed successfully and had molecular masses of 92 and 78 kDa, respectively. Both recombinant proteins were xyloglucanases. SaGH74A had optimal activity at 60°C and pH 5.5, while SaGH74B had optimal activity at 55°C and pH 6.0. SaGH74A was stable over a broad pH range (pH 4.5 to 9.0), whereas SaGH74B was stable over a relatively narrow pH range (pH 6.0 to 6.5). Analysis of the hydrolysis products of tamarind xyloglucan and xyloglucan-derived oligosaccharides indicated that SaGH74A was endo-processive, while SaGH74B was a typical endo-enzyme. The C terminus of SaGH74A, which was annotated as a carbohydrate-binding module, bound to β-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan.
Chemotaxis of Marinobacter adhaerens and Its Impact on Attachment to the Diatom Thalassiosira weissflogii

ABSTRACT Alga-bacterium interactions are crucial for aggregate formation and carbon cycling in aquatic systems. To understand the initiation of these interactions, we investigated bacterial chemotaxis within a bilateral model system. Marinobacter adhaerens HP15 has been demonstrated to attach to the diatom Thalassiosira weissflogii and induce transparent exopolymeric particle and aggregate formation. M. adhaerens possesses one polar flagellum and is highly motile. Bacterial cells were attracted to diatom cells, as demonstrated by addition of diatom cell homogenate or diatom culture supernatant to soft agar, suggesting that chemotaxis might be important for the interaction of M. adhaerens with diatoms. Three distinct chemotaxis-associated gene clusters were identified in the genome sequence of M. adhaerens, with the clusters showing significant sequence similarities to those of Pseudomonas aeruginosa PAO1. Mutations in the genes cheA, cheB, chpA, and chpB, which encode histidine kinases and methylesterases and which are putatively involved in either flagellum-associated chemotaxis or pilus-mediated twitching motility, were generated and mutants with the mutations were phenotypically analyzed. ΔcheA and ΔcheB mutants were found to be swimming deficient, and all four mutants were impaired in biofilm formation on abiotic surfaces. Comparison of the HP15 wild type and its chemotaxis mutants in cocultures with the diatom revealed that the fraction of bacteria attaching to the diatom decreased significantly for mutants in comparison to that for the wild type. Our results highlight the importance of M. adhaerens chemotaxis in initiation of its interaction with the diatom. In-depth knowledge of these basic processes in interspecies interactions is pivotal to obtain a systematic understanding of organic matter flux and nutrient cycling in marine ecosystems.
Examining the factors that influence the intentions to adopt internet shopping and cable television shopping in Taiwan

Using Rogers’ diffusion of innovation model, this study attempts to compare the adoption of internet shopping with that of cable television shopping in Taiwan. Rogers’ diffusion model has been criticized for its pro-innovation bias, and thus this study expects that the adoption of internet shopping will be consistent with the predictions of Rogers’ model, while the adoption of cable television shopping will not. A telephone survey with 1227 valid interviews was used to collect data for this study. As expected, most findings regarding internet shopping follow the predictions of this model. However, the diffusion process in relation to cable television shopping digresses from the path predicted by Rogers’ model. The detailed findings of the study are discussed in the article.
Political Economy, Power and New Media

This article suggests that it is timely to revitalise studies in the tradition of the political economy of media and communications in order to develop a critical and comprehensive analysis of the social and economic dynamics of the production and consumption of new media. Specifically, a coupling of research on mediated communication and on highly situated communities of practice with some strands of research in political economy could shed new light on the way changing power relationships are informing the development and application of new media products and services. There are precedents for this approach in studies of the older media and signs of a greater receptivity to such an approach in some studies of Internet developments and the open source software movement.
Rerouting Citrate Metabolism in Lactococcus lactis to Citrate-Driven Transamination

ABSTRACT Oxaloacetate is an intermediate of the citrate fermentation pathway that accumulates in the cytoplasm of Lactococcus lactis ILCitM(pFL3) at a high concentration due to the inactivation of oxaloacetate decarboxylase. An excess of toxic oxaloacetate is excreted into the medium in exchange for citrate by the citrate transporter CitP (A. M. Pudlik and J. S. Lolkema, J. Bacteriol. 193:4049–4056, 2011). In this study, transamination of amino acids with oxaloacetate as the keto donor is described as an additional mechanism to relieve toxic stress. Redirection of the citrate metabolic pathway into the transamination route in the presence of the branched-chain amino acids Ile, Leu, and Val; the aromatic amino acids Phe, Trp, and Tyr; and Met resulted in the formation of aspartate and the corresponding α-keto acids. Cells grown in the presence of citrate showed 3.5 to 7 times higher transaminase activity in the cytoplasm than cells grown in the absence of citrate. The study demonstrates that transaminases of L. lactis accept oxaloacetate as a keto donor. A significant fraction of 2-keto-4-methylthiobutyrate formed from methionine by citrate-driven transamination in vivo was further metabolized, yielding the cheese aroma compounds 2-hydroxy-4-methylthiobutyrate and methyl-3-methylthiopropionate. Reducing equivalents required for the former compound were produced in the citrate fermentation pathway as NADH. Similarly, phenylpyruvate, the transamination product of phenylalanine, was reduced to phenyllactate, while the dehydrogenase activity was not observed for the branched-chain keto acids. Both α-keto acids and α-hydroxy acids are known substrates of CitP and may be excreted from the cell in exchange for citrate or oxaloacetate.
Social Interactions Across Media

Two studies compared college students’ interpersonal interaction online, face-to-face, and on the telephone. A communication diary assessed the relative amount of social interactions college students conducted online compared to face-to-face conversation and telephone calls. Results indicated that while the internet was integrated into college students’ social lives, face-to-face communication remained the dominant mode of interaction. Participants reported using the internet as often as the telephone. A survey compared reported use of the internet within local and long distance social circles to the use of other media within those circles, and examined participants’ most recent significant social interactions conducted across media in terms of purposes, contexts, and quality. Internet interaction was perceived as high in quality, but slightly lower than other media. Results were compared to previous conceptualizations of the roles of internet in one’s social life.
Dermatoglyphic patterns of congenital lame adults in a southern Nigerian population.

Aim: This study is aimed at determining the dermatoglyphic patterns of the congenital lame subjects in a southern Nigerian population. Study Design: This is designed to identify the congenital lame subjects through their palmar and digital prints. Place and Duration of Study: The subjects were recruited from special schools in Owerri and Borikiri in Southern part of Nigeria. The study was conducted between the month of April to July, 2010. Materials and Method: A total number of 150 congenital lame subjects (comprising 100 males and 50 females) were recruited for this study. The parameters studied include the digital pattern, A-B ridge counts, ATD and DAT angles. Digital and palmar print patterns were obtained by using the ink method. Results: Results of this study showed that ulnar loops were the most predominant in both sexes.
Guided Wavelet Shrinkage for Edge-Aware Smoothing

Edge-aware smoothing has been extensively studied due to its wide range of applications in computer vision and graphics. Most published works have been focused on formulating the smoothing problem in the spatial domain. In this paper, we propose a new edge-aware smoothing framework called guided wavelet shrinkage (GWS), which is formulated in the wavelet domain as a maximum a posterior estimation problem. We impose a number of desirable properties on the statistical models and the associated parameters in order to derive an effective and computationally efficient algorithm. We compare the proposed GWS with classical image denoising in the wavelet domain. We have also compared different wavelet representations and found that the double-density dual-tree wavelet transform is the best choice. We show that the GWS can be configured as either self-guidance or external guidance. It can also be configured to operate in an iterative or non-iterative way. Experimental results and comparison with many stateof-the-algorithms demonstrate that the GWS-based algorithm can produce competitive results with O(N) computational complexity.
Use of a Foam Spatula for Sampling Surfaces after Bioaerosol Deposition

ABSTRACT The present study had three goals: (i) to evaluate the relative quantities of aerosolized Bacillus atrophaeus spores deposited on the vertical, horizontal top, and horizontal bottom surfaces in a chamber; (ii) to assess the relative recoveries of the aerosolized spores from glass and stainless steel surfaces with a polyester swab and a macrofoam sponge wipe; and (iii) to estimate the relative recovery efficiencies of aerosolized B. atrophaeus spores and Pantoea agglomerans using a foam spatula at several different bacterial loads by aerosol distribution on glass surfaces. The majority of spores were collected from the bottom horizontal surface regardless of which swab type and extraction protocol were used. Swabbing with a macrofoam sponge wipe was more efficient in recovering spores from surfaces contaminated with high bioaerosol concentrations than swabbing with a polyester swab. B. atrophaeus spores and P. agglomerans culturable cells were detected on glass surfaces using foam spatulas when the theoretical surface bacterial loads were 2.88 × 104 CFU and 8.09 × 106 CFU per 100-cm2 area, respectively. The median recovery efficiency from the surfaces using foam spatulas was equal to 9.9% for B. atrophaeus spores when the recovery was calculated relative to the theoretical surface spore load. Using a foam spatula permits reliable sampling of spores on the bioaerosol-exposed surfaces in a wide measuring range. The culturable P. agglomerans cells were recovered with a median efficiency of 0.001%, but staining the swab extracts with fluorescent dyes allowed us to observe that the viable cell numbers were higher by 1.83 log units than culturable organisms. However, additional work is needed to improve the analysis of the foam extracts in order to decrease the limit of detection of Bacillus spores and Gram-negative bacteria on contaminated surfaces.
Structure-Based Low-Rank Model With Graph Nuclear Norm Regularization for Noise Removal

Nonlocal image representation methods, including group-based sparse coding and block-matching 3-D filtering, have shown their great performance in application to low-level tasks. The nonlocal prior is extracted from each group consisting of patches with similar intensities. Grouping patches based on intensity similarity, however, gives rise to disturbance and inaccuracy in estimation of the true images. To address this problem, we propose a structure-based low-rank model with graph nuclear norm regularization. We exploit the local manifold structure inside a patch and group the patches by the distance metric of manifold structure. With the manifold structure information, a graph nuclear norm regularization is established and incorporated into a low-rank approximation model. We then prove that the graph-based regularization is equivalent to a weighted nuclear norm and the proposed model can be solved by a weighted singular-value thresholding algorithm. Extensive experiments on additive white Gaussian noise removal and mixed noise removal demonstrate that the proposed method achieves a better performance than several state-of-the-art algorithms.
INTERLEUKIN 1β, TUMOUR NECROSIS FACTOR-α AND INTERLEUKIN 1 RECEPTOR ANTAGONIST IN NEWLY DIAGNOSED INSULIN-DEPENDENT DIABETES MELLITUS: COMPARISON TO LONG-STANDING DIABETES AND HEALTHY INDIVIDUALS

Interleukin 1 beta (IL-1) and tumour necrosis factor alpha (TNF) are important for the beta cell lysis in insulin-dependent diabetes mellitus (IDDM), while IL-1 receptor antagonist (IL-1ra) is considered protective by blocking the effects of IL-1. Serum concentrations and ex-vivo production of IL-1, TNF and IL-1ra were examined in 10 newly diagnosed IDDM (ND-IDDM) patients, and compared with 11 long-standing IDDM (LS-IDDM) patients and 14 healthy volunteers. Ex-vivo LPS-stimulated production of IL-1 in ND-IDDM patients was significantly increased compared with LS-IDDM patients and healthy controls, while TNF and IL-1ra synthesis did not differ significantly. IL-1ra/IL-1 ratio was significantly decreased in ND-IDDM, and returned to normal values in the LS-IDDM group. Circulating concentrations of IL-1ra in LS-IDDM patients were increased. These data suggest a proinflammatory imbalance in ND-IDDM patients and this may play an important role in beta cell loss.