Miracle-dz/UniFAR-FTUs huggingface dataset:
Pomegranate Fruit Extract Impairs Invasion and Motility in Human Breast Cancer

Purpose. Pomegranate fruit extracts (PFEs) possess polyphenolic and other compounds with antiproliferative, pro-apoptotic and anti-inflammatory effects in prostate, lung, and other cancers. Because nuclear transcription factor-kB (NF-kB) is known to regulate cell survival, proliferation, tumorigenesis, and inflammation, it was postulated that PFEs may exert anticancer effects at least in part by modulating NF-kB activity. Experimental design. The authors investigated the effect of a novel, defined PFE consisting of both fermented juice and seed oil on the NF-kB pathway, which is constitutively active in aggressive breast cancer cell lines. The effects of the PFE on NF-kB—regulated cellular processes such as cell survival, proliferation, and invasion were also examined. Results. Analytical characterization of the bioactive components of the PFE revealed active constituents, mainly ellagitannins and phenolic acids in the aqueous PFE and conjugated octadecatrienoic acids in the lipid PFE derived from seeds.The aqueous PFE dose-dependently inhibited NF-kB—dependent reporter gene expression associated with proliferation, invasion, and motility in aggressive breast cancer phenotypes while decreasing RhoC and RhoA protein expression. Conclusion. Inhibition of motility and invasion by PFEs, coincident with suppressed RhoC and RhoA protein expression, suggests a role for these defined extracts in lowering the metastatic potential of aggressive breast cancer species.
Critical Pathways

Burgeoning concerns regarding patient safety, variable health care quality and increasing health care costs have led to the introduction of clinical management tools that have their origins outside of the traditional health care sector. Primary among these innovations has been the implementation of critical pathways, administrative models that streamline work and production processes. Critical pathways have been utilized extensively in several different business sectors including the construction and automotives industries. It is theorized that the adaptation of pathways to health care, particularly inpatient care, may help ensure the delivery of quality care and decrease the occurrence of medical errors.
Anesthesiologists, General Surgeons, and Tobacco Interventions in the Perioperative Period

Surgery presents an opportunity for interventions in cigarette smokers that will facilitate abstinence from tobacco. However, little attention has been paid to the role of anesthesiologists and surgeons in addressing tobacco use. To determine the practices and attitudes of these physicians regarding this issue, we sent a postal mail survey to a national random sampling of anesthesiologists and general surgeons engaged in active practice within the United States (1000 in each group). Response rates were 33% and 31% for anesthesiologists and surgeons, respectively. More than 90% of both groups almost always ask their patients about tobacco use, and almost all respondents believed that surgical patients should maintain abstinence after surgery. Most believed that it was their responsibility to advise their patients to quit smoking, but only 30% of anesthesiologists and 58% of surgeons routinely do so. Nonetheless, approximately 70% of both groups would be willing to spend an extra 5 min before surgery to help their patients quit. Barriers to intervention included a lack of training regarding intervention techniques, a perceived lack of effective interventions, and insufficient time to intervene. Intervention opportunities are not exploited consistently in the surgical population; educational efforts directed at physicians in surgical specialties are indicated.
Extraction and Characterization of Oil from Moringa oleifera Using Supercritical CO 2 and Traditional Solvents

The present work presents a first characterization of the oil from the Moringa (Moringa oleifera) kernel as a potential candidate for biodiesel production. Moringa is an indigenous tree in the Yucatan Peninsula in Mexico, where there is a nascent biodiesel industry. Several extraction methods are compared in terms of the extraction yields, including solvent extraction (n-hexane and ethanol), and supercritical extraction (Sc-CO2). The results are also compared against previ- ously reported data. For supercritical extraction pressures of 200 to 400 bar and temperatures of 40℃ and 60℃ were tested. Gas Chromatography analysis reveals that the main fatty acids in Moringa oil are oleic acid (69%), palmitic acid (10%), and stearic acid (8%).
THE EFFECT OF Nano-MgO ON THE MECHANICAL AND FLAMMABILITY PROPERTIES OF HYBRID NANO COMPOSITES FROM WOOD FLOUR-POLYETHYLENE

This study considered the effect of nanomagnesium oxide on the mechanical and flammability features of composites made of wood flour and high-density polyethylene. A sample of wood flour was made from the mixture of hardwoods and high density polyethylene with the weight ratio of 50%. Maleic anhydride was added as a compatibilizer (2 phc), and nanomagnesium oxide was applied at 6 levels (0, 1, 2, 3, 4, 5 phc). These materials were mixed, and samples were prepared with determined sizes by injection molding machine. The samples were subjected to flexural tests to examine the mechanical features, and to study flammability strength, various tests were conducted with a cone calorimeter, including the amount of char residue, total smoke production, time to ignition, and heat release rate, according to ASTM E1354-92 (1992). The addition of up to 3 phc nanomagnesium oxide increased flexural strength and modulus, but further additions decreased these values. The addition of 5 phc nanomagnesium oxide increased the char residue and ignition time, and it decreased the heat release rate, total smoke production, and burning rate. Scanning electron microscopy and energy dispersive X-ray (EDX) analysis indicated the improper transmittance of nanomagnesium oxide and accumulations in the samples.
Anthocyanins from Hibiscus sabdariffa, Melastoma malabathricum and Ipomoea batatas and its color properties

There are many factors influencing the stability and color variation of natural colorant anthocyanin and pH is among the most significant factor. This study aims to determine the stability of the anthocyanins in freeze-dried Hibiscus sabdariffa, Melastoma malabathricum and Ipomoea batatas in various acidic pH (pH 2.0, 2.5, 3.0, 3.5, 4.0 and 4.5). Total monomeric anthocyanin, degradation index, color density and percent polymeric color were analyzed to determine anthocyanins degradation and their color variations. Among the samples, H.sabdariffa contain the highest monomeric anthocyanins (163.3 mg/L) followed by M. malabathricum (49.9 mg/L) and the lowest is I.batatas (13.8 mg/L). Monomeric anthocyanins from I.batatas were found to be very stable and not affected by changes in pH than in H. sabdariffa and M. malabathricum. However, degradation index (DI) of H. sabdariffa was the lowest with value of 0.365 ± 0.049 at pH 3.5. The lowest percentage of polymeric color (4.94 ± 0.64) was also shown by H. sabdariffa at pH 2.5 and maintained a deep red color with increasing pH indicating higher color stability compared to M. malabathricum and I. batatas. Overall, natural pigment in H. sabdariffa was found to be the most stable in both monomeric anthocyanin content and chromaticity properties. These results provided information that will further proven the potential usage of H. sabdariffa, M. malabathricum and I. batatas as natural coloring agents to replace the synthetic colorant in food and beverage industries.
Evolutionary Origins of the Photosynthetic Water Oxidation Cluster: Bicarbonate Permits Mn2+ Photo‐oxidation by Anoxygenic Bacterial Reaction Centers

The enzyme that catalyzes water oxidation in oxygenic photosynthesis contains an inorganic cluster (Mn4CaO5) that is universally conserved in all photosystem II (PSII) protein complexes. Its hypothesized precursor is an anoxygenic photobacterium containing a type 2 reaction center as photo‐oxidant (bRC2, iron–quinone type). Here we provide the first experimental evidence that a native bRC2 complex can catalyze the photo‐oxidation of Mn2+ to Mn3+, but only in the presence of bicarbonate concentrations that allows the formation of (bRC2)Mn2+(bicarbonate)1–2 complexes. Parallel‐mode EPR spectroscopy was used to characterize the photoproduct, (bRC2)Mn3+(CO32−), based on the g tensor and 55Mn hyperfine splitting. (Bi)carbonate coordination extends the lifetime of the Mn3+ photoproduct by slowing charge recombination. Prior electrochemical measurements show that carbonate complexation thermodynamically stabilizes the Mn3+ product by 0.9–1 V relative to water ligands. A model for the origin of the water oxidation catalyst is presented that proposes chemically feasible steps in the evolution of oxygenic PSIIs, and is supported by literature results on the photoassembly of contemporary PSIIs.
Stress of urban pollution on largest natural wetland ecosystem in East Kolkata-causes, consequences and improvement

The traditional practice of utilizing wastewater into fish pond is a unique example of sustainable socio-economic development pertaining to resource recovery in the Eastern Kolkata wetlands, a Ramsar site in India. This paper revealed the stress of urban pollution and poor land use planning on the world’s largest natural wetland. This is the first time to critically evaluate dynamics of oxygen demanding substances, nutrients and solids in waste water canal and fish ponds. This paper sheds some lights on absorbance of oxygen demanding substances on TSS and their adverse impact on fish ponds. Based on this concern, barriers to maintain water quality of wastewater canal and fish ponds; and opportunities to improve resource recovery efficiency have been explored. This study also explored that the radical technological change for fish pond management would not be effective in the prevailing socio-economic condition. But incremental changes and proper coordination between the concerned authority and fish farmers can easily accelerate the fish yield, functionality of pond ecosystem and reduction of pollutants.
Lead-induced oxidative stress adversely affects health of the occupational workers

Lead is a persistent toxic metal and associated with impairment of various body functions in occupational workers. The main objective was to determine the lead-induced oxidative stress and adverse health effects by biochemical markers in industrial workers. One hundred and forty-eight males consisting of 87 lead-exposed industrial workers and 61 controls were included. Blood lead level (BLL) was determined on a 3010B ESA lead analyzer. Blood complete counts were done on a hematology analyzer. Biochemical markers including serum uric acid, urea, creatinine, phosphate, alanine aminotransferase (ALT), and gamma glutamyltransferase (GGT) were measured on a Selectra E auto analyzer. Serum malondialdehyde (MDA) was measured spectrophotometrically and C-reactive protein (CRP) on Immulite-1000. Results revealed that lead-exposed workers had significantly high BLLs, median (range), 29.1 (9.0–61.1) μg/dL compared with controls, 8.3 (1.0–21.7) μg/dL. Oxidative stress (MDA, GGT) and inflammatory markers (high-sensitivity CRP) were significantly increased (P ≤ 0.05). Blood pressure was raised, whereas hemoglobin was decreased in exposed group (P ≤ 0.002). Serum urea, uric acid, phosphate, and ALT were significantly raised in lead-exposed workers (P ≤ 0.001). Serum albumin, total proteins, and glomerular filtration rate (GFR) were decreased. Blood lead showed a significant positive correlation with serum GGT (r = 0.63), MDA (r = 0.71), CRP (r = 0.75), urea (r = 0.34), creatinine (r = 0.51), and uric acid (r = 0.29) (P ≤ 0.01). It is concluded that lead exposure increases oxidative stress that correlates with adverse changes in hematological, renal, and hepatic function in the occupational workers. Elevated blood lead has positive correlation with oxidative stress, inflammatory and biochemical markers that might be used to detect impairment in the body function in lead exposed workers.
Deep Belief Networks for Automatic Music Genre Classification

This paper proposes an approach to automatic music genre clas-siﬁcation using deep belief networks. Based on the restricted Boltzmann machines, the deep belief networks is constructed and takes the acoustic features extracted through content-based analysis of music signals as input. The model parameters are initially determined after the deep belief network is trained by greedy layer-wise learning algorithm with feature vectors that are comprised of short-term and long-term features. Then the parameters are ﬁne-tuned to local optimum according to back propagation algorithm. Experiments on GTZAN dataset show that the performance of music genre classiﬁcation using deep belief networks is superior to those of widely used classiﬁcation methods such as support vector machine, K -nearest neighbor, linear discriminant analysis and neural network.
Information Management Capability as Competitive Imperfection in the Strategic Factor Market of Big Data

The interest of the organizations in developing Big Data strategies is increasing significantly. However, the expectation of the value of these benefits and of the costs involved in acquiring or developing these solutions are not homogeneous for all of the firms, generating competitive imperfections in the market of strategic resources. Using Information Management Capability (IMC) as a premise to provide the required unique insight for Big Data strategies to be successful, this article proposes to analyze IMC as an imperfection agent in the market of strategic resources of Big Data. The formulated hypotheses were tested from a survey of 101 valid participants and analyzed with SEM-PLS. The results indicate a positive IMC influence on value expectation and a negative one on cost expectation. Cost expectation inversely affects the intent to purchase or develop the resources to implant Big Data strategies. Value expectation has a positive effect in both intents.
Triad3A Regulates Ubiquitination and Proteasomal Degradation of RIP1 following Disruption of Hsp90 Binding*

Toll-like receptors (TLRs) play a crucial role in innate immunity by recognizing microbial pathogens. Triad3A is an E3 ubiquitin-protein ligase that interacts with the Toll/interleukin-1 receptor domain of TLRs and promotes their proteolytic degradation. In the present study, we further investigated its activity on signaling molecules downstream of TLRs and tumor necrosis factor (TNF) receptor 1. Triad3A promoted down-regulation of two TIR domain-containing adapter proteins, TIRAP and TRIF, as well as a RIP1 but had no effect on other adapter molecules in either the TLRs or TNF-α signaling pathways. Multiple sequence alignment analysis suggested that RIP1 contains a TIR homologous domain, and mutation of amino acid residues in this domain identified three residues critical for its interaction with Triad3A. Moreover, Triad3A acted as a negative regulator in TNF-α signaling. Reduction of Triad3A expression by small interference RNAs rendered cells hyperresponsive to TNF-α stimulation. Conversely, overexpression of Triad3A in cells blocked TNF-α-induced cell activation. This negative regulation was effected independently of changes in the cellular protein level of RIP1. Further studies indicated that RIP1 formed a complex with Triad3A and heat shock protein 90 (Hsp90), which is a chaperone protein capable of maintaining the stability of its client proteins. Treatment of cells with geldanamycin to disrupt the Hsp90 complex led to proteasomal degradation of RIP1. Depletion of Triad3A by small interference RNA treatment inhibited geldanamycin-activated ubiquitination and proteolytic degradation of RIP1. These results suggest that Triad3A is an E3 ubiquitin-protein ligase to RIP1 and that Hsp90 and Triad3A cooperatively maintain the homeostasis of RIP1.
Parasitics assisted soft-switching and secondary-clamped current-fed bidirectional voltage doubler for fuel cell vehicles

A novel current-fed secondary-assisted clamped zero current commutated bidirectional full-bridge voltage doubler is proposed for fuel cell vehicles (FCVs). The proposed topology with novel modulation is suitable for interfacing energy storage with fuel cell or dc bus in FCVs. Voltage doubler on secondary side is selected to enhance the gain by 2x and reduce transformer size. Parasitics based zero voltage switching (ZVS), secondary based zero current switching (ZCS) of low voltage side devices and ZVS of secondary devices are achieved. Proposed secondary modulation technique naturally clamps the voltage across the primary side devices with zero current commutation eliminating the necessity for traditional active-clamp or passive snubbers. Switching losses are reduced significantly owing to soft-switching of all semiconductor devices. Synchronous rectification may be implemented to obtain high efficiency. Soft-switching and voltage-clamping are inherent and achieved at variation in load and source voltage. Steady state operation, analysis, design, simulation and experimental results are presented in this paper.
Acute stimulation of steroidogenesis in corpus luteum and adrenal cortex by peptide hormones. Rapid induction of a similar protein in both tissues.

Two-dimensional electrophoresis was used to detect a protein (ic) synthesized in rat corpus luteum cells in response to acute stimulation by human chorionic gonadotropin or dibutyryl cyclic AMP. This induced protein ic is isoelectric at pH 6.5 (isoelectric focusing) and has an apparent molecular weight of 28,000 (sodium dodecyl sulfate electrophoresis). The human chorionic gonadotropin or dibutyryl cyclic AMP dose response and time course of synthesis of the protein parallel those of progesterone synthesis in stimulated luteal cells. Additionally, cycloheximide, which inhibits the increase in progesterone formation caused by human chorionic gonadotropin or cAMP, also inhibits the synthesis of ic. Proteolytic polypeptide mapping suggests that ic has a very similar primary structure to another protein (pc), which has the same molecular weight as ic, differs from ic in pI, and is synthesized only in unstimulated cells. These polypeptide maps also demonstrate the close similarity of pc and ic to two proteins p and i, synthesized in control and in adrenocorticotropic hormone-stimulated rat adrenal cortex cells, respectively (Krueger, R. J. and Orme-Johnson, N. R. (1983) J. Biol. Chem. 258, 10159-10167). In both adrenal cortex and corpus luteum, binding of a tissue-specific polypeptide hormone acts via cAMP to cause increased steroidogenesis and induction of the synthesis of protein i (ic), with the same time course and hormone dose dependence. Also in both tissues, inhibition of protein synthesis at the level of translation (e.g. by cycloheximide addition) causes inhibition of i (ic) synthesis and of stimulated steroid production. This close correlation between the two different tissues in conditions which cause induction of the synthesis of these proteins suggests that the proteins may be common intermediaries in the control by polypeptide hormones of steroidogenesis in endocrine tissues.
[Correlation of somatostatin receptor expression in human hepatocellular carcinoma tissue to serum alpha-fetoprotein concentration].

BACKGROUND & OBJECTIVE Somatostatin analogue (SSTA) has inhibitory effect on the growth of hepatocellular carcinoma (HCC). However, the expression of somatostatin receptor (SSTR) in HCC is still unclear and its correlation to serum alpha-fetoprotein (AFP) has not yet been explored. This study was to investigate the expression of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5 subtypes in HCC, and explore their correlations to serum AFP concentration. METHODS The mRNA and protein expression of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5 subtypes in 40 specimens of HCC and 40 specimens of liver cirrhosis were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC), respectively. AFP levels in liver tissues and peripheral blood of the 40 HCC patients were measured with enzyme-linked immunosorbent assay (ELISA). RESULTS The positive rates of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5 proteins were 47.5%, 70.0%, 50.0%, 65.0%, and 67.5% in HCC, and 55.0%, 67.5%, 52.5%, 60.0%, and 47.5% in liver cirrhosis tissues, respectively. The protein levels of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5 were markedly higher in HCC than in liver cirrhosis tissues. The expression of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5 in HCC had curilinear correlations with serum AFP levels (r = 0.882, 0.901, 0.877, 0.854, 0.903, respectively, P < 0.05). Serum AFP levels ranging between 200-800 ng/ml were associated with high expression of SSTRs in HCC; serum AFP levels below or over that range were associated with low expression of SSTRs. CONCLUSIONS About 60% of the HCC tissues express SSTRs; the protein levels of SSTRs are much higher in HCC than in liver cirrhosis tissues. HCC patients with serum AFP level ranging between 200-800 ng/ml may be good candidates for SSTA therapy.
The Response of Relatives to Medicolegal Investigations and Forensic Autopsy

Relatives of deceased persons, on whose body a forensic autopsy had been performed at the Institute of Forensic Science, Bern, Switzerland, were interrogated by a questionnaire. The aim was to investigate the attitude of relatives toward medicolegal investigation procedures in general and toward forensic autopsy in particular. A great majority of the relatives showed a positive or indifferent attitude toward a forensic autopsy. They showed a great interest in autopsy results and wished to be informed. It was concluded that information given before the autopsy is important for better understanding and can, if properly given, improve the relatives’ acceptance and collaboration regarding forensic investigations. By contrast, a lack of information before the autopsy and about the autopsy results can cause further suffering on behalf of the bereaved. A forensic autopsy can be of great benefit for relatives and can help them to cope with a tragic loss.
SPGM: an efficient algorithm for mapping MapReduce-like data-intensive applications in data centre network

In traditional data centre network, how to efficiently allocate the virtual data centres VDCs on the physical data centre network PDCN is a challenging problem, which is denoted as GraphMap. GraphMap refers to map the virtual nodes to the substrate nodes and the virtual links to the substrate paths, respectively. The existing heuristic approaches attempt a two stage solution by solving the node mapping in a first stage and doing the link mapping in a second stage, which results in the mapping time being very large. In this paper, we propose an efficient mapping algorithm based on shortest path graph matching SPGM for online MapReduce-like data-intensive applications; the simulations show that SPGM can efficiently allocate the MapReduce-like data intensive applications on the PDCN in a much shorter time compared to the existing heuristic algorithms and maintain good performance.
31 P Nuclear Magnetic Resonance and Zero-Point Titration Compared for Measuring Free Magnesium Concentration in Erythrocytes

try, and3 Physiology, University Hospital Nijmegen, University of Nijmegen, 6500 HB Numegen, The Netherlands. 4Laboratory of Biophysical Chemistry and SON National HF NMR Facility, University of Nijmegen. 5Department of Hematology, Laboratory of Medical Enzymology, University Hospital Utrecht, Utrecht, The Netherlands. 6Nonstandard abbreviations: [Mg], ionized free intracellular magnesium concentration; [MgTI1, intracellular total magnesium concentration; [Mgi0, ionized magnesium concentration in the medium at equilibration; NMR, nuclear magnetic resonance; ZPT, zero-point titration; EGTA, ethylene glycol bis(-aminoethyl ether)-N,N,N’,N’-tetraacetic acid; DPG, 2,3-diphosphogluconate; and ‘I’, fraction of free (noncomplexed) ATP. Received April 8, 1991; accepted October 10, 1991. CLIN.CHEM.37/12,2076-2080 (1991)