Miracle-dz/UniFAR-FTUs huggingface dataset:
Simultaneous Glycan-Peptide Characterization Using Hydrophilic Interaction Chromatography and Parallel Fragmentation by CID, Higher Energy Collisional Dissociation, and Electron Transfer Dissociation MS Applied to the N-Linked Glycoproteome of Campylobacter jejuni*

Campylobacter jejuni is a gastrointestinal pathogen that is able to modify membrane and periplasmic proteins by the N-linked addition of a 7-residue glycan at the strict attachment motif (D/E)XNX(S/T). Strategies for a comprehensive analysis of the targets of glycosylation, however, are hampered by the resistance of the glycan-peptide bond to enzymatic digestion or β-elimination and have previously concentrated on soluble glycoproteins compatible with lectin affinity and gel-based approaches. We developed strategies for enriching C. jejuni HB93-13 glycopeptides using zwitterionic hydrophilic interaction chromatography and examined novel fragmentation, including collision-induced dissociation (CID) and higher energy collisional (C-trap) dissociation (HCD) as well as CID/electron transfer dissociation (ETD) mass spectrometry. CID/HCD enabled the identification of glycan structure and peptide backbone, allowing glycopeptide identification, whereas CID/ETD enabled the elucidation of glycosylation sites by maintaining the glycan-peptide linkage. A total of 130 glycopeptides, representing 75 glycosylation sites, were identified from LC-MS/MS using zwitterionic hydrophilic interaction chromatography coupled to CID/HCD and CID/ETD. CID/HCD provided the majority of the identifications (73 sites) compared with ETD (26 sites). We also examined soluble glycoproteins by soybean agglutinin affinity and two-dimensional electrophoresis and identified a further six glycosylation sites. This study more than doubles the number of confirmed N-linked glycosylation sites in C. jejuni and is the first to utilize HCD fragmentation for glycopeptide identification with intact glycan. We also show that hydrophobic integral membrane proteins are significant targets of glycosylation in this organism. Our data demonstrate that peptide-centric approaches coupled to novel mass spectrometric fragmentation techniques may be suitable for application to eukaryotic glycoproteins for simultaneous elucidation of glycan structures and peptide sequence.
How technology makes us human: cultural historical roots for design and technology education

Abstract In the context of curriculum change within English education, and beyond, this article explores the cultural historical roots of design and technology as an educational construct, distinct from design or engineering, which exist as career paths outside of the school curriculum. It is a position piece, drawing on literature from a wide range of sources from writing, largely, outside of the discipline. The authors revisit the original intentions of design and technology as a National Curriculum subject and, within the contemporary challenges, discuss the importance of technology, including designing and making, as an essentially human and humanising activity. The aim being to contribute to the theorisation and philosophy of the subject, where typically practitioners focus on practical and potentially mundane concerns. This article asserts that technological human activity is rooted in technological innovation and determinism, inextricably linked to social human activity. The aim is to add to the literature and provoke debate around the place and value of design and technology. The argument for retention of the subject, as part of a broad and balanced curriculum, is presented from a social and technological perspective; recognising the value of the subject as cultural rather than a merely technical or economic imperative.
Clinical study of two brothers with a novel 33 bp duplication in the ARX gene

Pathogenic variations of the ARX (aristaless‐related homeobox) gene are associated with marked phenotypic pleiotropy. These phenotypes are X‐linked neurological disorders that include brain and genital malformation and non‐malformation syndromes. Typically, malformation phenotypes result from pathogenic variations that are predicted to truncate the ARX protein, or alter residues in the highly conserved homeodomain. While non‐malformation phenotypes tend to be caused by pathogenic variations that are predicted to expand the first two polyalanine tracts of ARX, or alter residues outside of the homeodomain. The most common pathogenic variation of the ARX gene is a duplication of 24 bp, c. 429_452 dup, which leads to an expansion of the second polyalanine tract of the ARX protein from 12 to 20 alanine residues. This pathogenic variation is associated with both sporadic and familial nonsyndromic mental retardation. Syndromic manifestations include mental retardation with hand dystonia (Partington syndrome), infantile spasms (West syndrome) and/or other epileptic seizures. Here, we report on a novel pathogenic variant of a tandem 33 bp duplication that is predicted to result in an expansion of polyalanine tract 2 in two brothers with mental retardation, epilepsy, dystonia, and the novel feature of intermittent hyperventilation. This pathogenic variation is predicted to result in a “non‐homogeneous” polyalanine tract expansion that is longer than predicted expansion caused by the common 24 bp duplication. The location of the novel 33 bp duplication in the same region as the common 24 bp duplication supports this region as the ARX variation “hot spot. ” © 2009 Wiley‐Liss, Inc.
The Clinical Syndrome Associated with Lumbar Spinal Stenosis

Lumbar spinal stenosis is well defined in patho-anatomical terms but its clinical features are heterogeneous. We carried out a comprehensive retrospective review of the clinical features, radiological changes and outcome of 75 patients with radiologically diagnosed lumbar spinal stenosis in order to define its clinical spectrum. The presenting complaints were of weakness, numbness/tingling, radicular pain and neurogenic claudication in almost equal proportions. The commonest symptom was numbness or tingling of the legs. Neurogenic claudication eventually occurred in only 61%. Ninety-three per cent showed abnormalities on neurological examination, but these were generally mild with reduced ankle jerks being commonest. Imaging of the lumbar spine showed that moderate to severe central spinal stenosis correlated with complaints of weakness and abnormal motor power on clinical examination. Patients were reviewed at a mean of 4 years after diagnosis and 65% had undergone surgical decompression; this was not a prospective comparison of different treatment modalities. Overall, a third of patients felt that their symptoms had improved while a quarter felt that they had worsened. More than half had satisfactory neurological function at the time of review. Thirty-nine per cent of those treated surgically, and 25% of those managed conservatively, reported improved symptoms. A poorer functional status at review correlated with complaints of motor weakness and associated comorbid disease. Degenerative lumbar stenosis is a clinically heterogeneous neurological disorder of the lower limbs in the elderly with variable longer-term outcome. A high index of suspicion is required and neuroimaging should be obtained to confirm the diagnosis.
In vitro antioxidant, antimicrobial and anti-proliferative activities of purple potato extracts (Solanum tuberosum cv Vitelotte noire) following simulated gastro-intestinal digestion

Analyses of antioxidant and in vitro antimicrobial and anti-proliferative activities of anthocyanin-rich extracts from purple potatoes, Solanum tuberosum L. cv Vitelotte noire (Solanaceae), were performed by simulating both a domestic cooking process and human digestion. Extracts of crude and cooked purple potato did not exhibit antimicrobial activity against the tester strains: Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The behaviour changed after the simulated gastrointestinal transit, when an inhibition halo was observed against all tester strains used, ranging from 0.53 cm against B. cereus to 0.82 cm against E. coli. In addition antioxidant activity exhibited, before and after the simulated gastrointestinal digestion (5.96 mg/mL ± 0.92; 28 mg/mL ± 0 .13, respectively) and the persistence of anti-proliferative activity against the colon cancer cells Caco-2, SW48 and MCF7, MDA-MB-231 breast cancer cells, after the simulated digestion, (EC50 = 0.21; 1.13 μg/mL), suggest that vitelotte consumption might bring tangible benefits for human health.
Airport Slots and the Internalization of Congestion By Airlines: An Empirical Model of Integrated Flight Disruption Management in Brazil

Abstract This paper examines the relationship between the control of airport slots by major airlines and their incentives to engage in service quality. We investigate a set of airline strategies regarding possible practices of slot hoarding and slot concentration through mergers aiming at erecting airport barriers to entry. We develop an econometric model of flight disruptions by allowing an integrated management of flight delays, cancellations, aircraft size, price and passengers per flight. We consider the case of the domestic airline industry in Brazil. We find evidence of the internalization of congestion externalities by dominant carriers. We also have some evidence of schedule padding, a strategic trade-off between delays and cancelations, and slot hoarding following a merger. Our results suggest that carriers intensify the internalization of congestion externalities when slot flight concentration increases.
Effect of phosphate and sediment bacteria on trichloroethylene dechlorination with zero valent iron

The effects of sediment-isolated bacteria and phosphate on the efficacy of zero valent iron (ZVI) for the dechlorination of trichloroethylene (TCE) were examined in batch experiments. TCE (0.3 mM) and a constant concentration of sediment bacteria were simultaneously exposed to ZVI in the presence of 0 mmol, 15 mmol, and 30 mmol of phosphate. TCE profiles, starting from 0.3 mM to about 0.1 mM, exhibited two-phase of sorption kinetics at all three phosphate concentrations without the sediment bacteria. TCE removal was less and slower with phosphate in the system. With the sediment bacteria, however, more TCE was removed with the sediment bacteria than without it, unlike our initial hypothesis. With the sediment bacteria and phosphate, the concentration of ferrous (0.505 mM) ions was doubled that with phosphate only (0.271 mM). The sediment bacteria in this research, mainly Bacillus sp. , could contribute to the long-term stability of ZVI reactivity for dechlorination of TCE in sediment. The sediment bacteria in this research could reduce the iron or chelate the evolved ferrous ions to retain the reducing reactivity of ZVI.
Trimeric Autotransporter Adhesin-Dependent Adherence of Bartonella henselae, Bartonella quintana, and Yersinia enterocolitica to Matrix Components and Endothelial Cells under Static and Dynamic Flow Conditions

ABSTRACT Trimeric autotransporter adhesins (TAAs) are important virulence factors of Gram-negative bacteria responsible for adherence to extracellular matrix (ECM) and host cells. Here, we analyzed three different TAAs (Bartonella adhesin A [BadA] of Bartonella henselae, variably expressed outer membrane proteins [Vomps] of Bartonella quintana, and Yersinia adhesin A [YadA] of Yersinia enterocolitica) for mediating bacterial adherence to ECM and endothelial cells. Using static (cell culture vials) and dynamic (capillary flow chambers) experimental settings, adherence of wild-type bacteria and of the respective TAA-negative strains was analyzed. Under static conditions, ECM adherence of B. henselae, B. quintana, and Y. enterocolitica was strongly dependent on the expression of their particular TAAs. YadA of Y. enterocolitica did not mediate bacterial binding to plasma or cellular fibronectin under either static or dynamic conditions. TAA-dependent host cell adherence appeared more significant under dynamic conditions although the total number of bound bacteria was diminished compared to the number under static conditions. Dynamic models expand the methodology to perform bacterial adherence experiments under more realistic, bloodstream-like conditions and allow dissection of the biological role of TAAs in ECM and host cell adherence under static and dynamic conditions.
Immunomodulatory Properties of FK734, a Humanized Anti-CD28 Monoclonal Antibody With Agonistic and Antagonistic Activities

Background. We describe immunomodulatory effects of FK734, a humanized version of a mouse anti-human CD28 mAb (clone TN228), in vitro and in a chimeric human–mouse model of allograft rejection. Methods. Cytokine production and proliferation were assessed in a mixed lymphocyte reaction containing FK734, human T cells, and endothelial cells or monocytes. FK734 was also administered to SCID mice engrafted with human skin and adoptively transferred with human peripheral blood mononuclear cells allogeneic to the skin graft. Results. In vitro, FK734 enhanced secretion of interleukin-2 and interferon-&ggr; as well as proliferation of CD4+ and CD8+ T cells stimulated by allogeneic human leukocyte antigen (HLA)-DR+ human umbilical vein endothelial cells (which lack B7 molecules and Fc&ggr;Rs) or by blood monocytes (which express low levels of B7 molecules and Fc&ggr;Rs) compared with control mAb, but these effects were significantly smaller than those provided by mAb 28.2, a stimulatory mouse anti-human CD28 mAb, at comparable concentrations. However, FK734 generally inhibited cytokine secretion and T cell proliferation in cocultures with human umbilical vein endothelial cells transduced to express CD86. In vivo using SCID/beige mice bearing human skin with adoptively transferred peripheral blood mononuclear cells, administration of FK734 protected human endothelial cell-lined microvessels, significantly but incompletely reducing endothelial cell injury and T cell infiltration into the graft one or two weeks later. Conclusions. FK734 is a partial agonist of CD28 signaling that can reduce human T cell alloresponses in the presence of strong costimulation by B7 molecules in vitro and can reduce T cell-mediated skin allograft rejection in vivo.
The contingent role of dependency in predicting the intention to adopt B2B e-commerce

ABSTRACT A review of existing Electronic Commerce adoption literature reveals that the prevailing theoretical frameworks implicitly assume that organizations are independent and have complete control over their decisions to adopt innovations. Given the fact that organizations are often constrained by and depend on their environment and exchange partners to survive, this paper proposes that Resource Dependency can either be a facilitator or inhibitor of the role of Technological, Organizational, and Environmental (TOE) motivating factors. Using 239 valid responses from supply chain managers in Jordan, the study found partial support for the role of Resource Dependency as a moderator. The findings provide insights on how Resource Dependency between partners drives different patterns of innovation acceptance among potential adopters, regardless of several technological and organizational motivations to innovate. The results suggest that predicting how innovation is diffused within a market needs a detailed understanding of the inter-dependence structure among the supply chain members.
Different hemispheric specializations for pitch and audioverbal working memory

Critical times of involvement of areas important to working memory were examined both with pitch and audioverbal N-back tasks using single-pulse transcranial magnetic stimulation (TMS). TMS was administered to 12 healthy participants over dorsolateral prefrontal and inferior parietal regions in each hemisphere at four different times after stimulus onset (250, 450, 650, and 850 ms). For the pitch N-back task, interference with working memory, as evidenced by a significant increase in reaction time, was observed with TMS over the right hemisphere regions. In contrast, for the audioverbal N-back task, TMS resulted in significantly increased reaction time only for left inferior parietal TMS delivered 450 ms after stimulus onset. These results imply different hemispheric specializations for pitch and audioverbal working memory.
Heart knowledge: towards (w)holistic ecoliteracy in teacher education

Despite repeated calls internationally, nationally and provincially to place the development of ecoliteracy as a curricular priority, there continues to be a lack of attention provided towards this goal, in particular opportunities for direct contact with the natural world in terms of fostering ecoliteracy in student teachers (Tuncer, 2009; Davis, 2009, Gough, 2009, Beckford, 2008; Blanchet-Cohen & Elliot, 2011). Teachers play key roles in advancing environmental education efforts and the environmental literacy of future generations. Insufficient teacher preparation has been identified as one factor in the weakness of environmental education efforts and environmental education curriculum (Beckford, 2008; Lin, 2002; Knapp, 2000). Furthermore, adequate environmental education preparation of students in teacher-training programs is essential for helping future teachers design and implement effective environmental education curriculum (Cutter-Mackenzie and Smith, 2003; Mc Keown-Ice, 2000; Spork, 1992). Future generations of students need to begin to perceive themselves, once again, in terms of
Impact of role models on the entrepreneurial intentions of engineering students

This paper reports the impact of role models on the entrepreneurial intentions of engineering students in Hong Kong. The research is based on theory of planned behavior (TPB) and empirical data of 411 engineering students from three universities. The results reveal that, no matter students took an entrepreneurship course or not, there are significant differences in entrepreneurial intentions between students with and without role models like entrepreneurial parents, relatives or friends. In other words, our local entrepreneurship courses do not compensate for the lacking of role models. Implications for designing new entrepreneurship courses will be explored.
Homocysteine concentration and the risk of death in the adult Polish population.

BACKGROUND Although there is a considerable epidemiologic evidence for a relation between homocysteine (Hcy) level and cardiovascular disease (CVD). The role of Hcy as a causal risk factor remains controversial. AIM To determine associations between Hcy level and all-cause and cardiovascular mortality in general population of Poland. METHODS Within the frame of the National Multicenter Health Survey (WOBASZ), a representative sample of whole Polish population aged 20-74 was screened in years 2003-2005 and prospectively followed up until 2009. Baseline determinations, among other classical risk factors, included Hcy level in 7165 responders, performed by an immunoenzymatic method using IMMULITE 1 analyser and DPC reagents. Survival rates were followed up until 2009 and average follow up time was 5.4 years. RESULTS During the 38,818.9 person-years of follow-up there were 270 deaths including 108 due to CVD, 37 due to coronary heart disease and 21 due to stroke. The relative risk of all-cause and CVD mortality was significantly higher in the highest (> 10.51 μmol/L) compared to the lowest (< 8.20 μmol/L) Hcy tercile in crude and multivariable proportional hazards models adjusted for sex, age, smoking status, hypertension, body mass index, total cholesterol, glucose and high sensitivity-C-reactive protein. Hazards ratios (95% confidence intervals) were as follows: all-cause mortality HR (95% CI): crude = 4.528 (2.947-6.154), multivariable-adjusted = 1.766 (1.197-2.605), CVD mortality crude = 4.322 (2.426-7.700), multivariable- -adjusted = 1.937 (1.051-3.569). CONCLUSIONS In Polish adult population Hcy concentration is independently associated with all-cause and CVD mortality.
Work Engagement among Women Engineers in Malaysia

This research aims to examine the effects of EffortReward Imbalance on work engagement among women engineers in Malaysia. In this study we review the Effort-Reward Imbalance (ERI) model as the psychosocial work environment that consisted of three distinct dimensions, namely effort, reward and over-commitment. In this study, a total of 300 questionnaires were distributed to women engineers, but only 250 questionnaires have been completed by the respondents. The data were analyzed using correlation and multiple regression through SPSS version 22. The findings showed that all the three dimensions of ERI model (i.e. effort, rewards, and over commitment) are related to work engagement among women engineers. This study indicated that reward is the most dominant influence work engagement among women engineers. This paper discusses the implication of this study in the context of literature and suggests strategies to improve workplace engagement. Keywords—ERI Model; Work engagement; Women Engineer
Consensus statement on the use of intravenous immunoglobulin therapy in the treatment of autoimmune mucocutaneous blistering diseases.

OBJECTIVES The purpose of the meeting of the Consensus Development Group was to critically evaluate the current published data on the use of intravenous immunoglobulin (IVIg) therapy in the treatment of autoimmune mucocutaneous blistering diseases (AMBDs) and to discuss the industrial preparation and safety features of this biologic agent. PARTICIPANTS The participants were physicians who frequently treat patients with these diseases and included dermatologists, oral medicine specialists, ophthalmologists, and immunologists. The members of the group provided input and discussion in their areas of expertise. The participants were invited attendees. EVIDENCE Data samples included only published information in the English-language literature. The expert opinions and experience of the members of the Consensus Development Group were vital to the discussion. CONSENSUS PROCESS A consensus was achieved by an open discussion and cumulative agreement on all issues relevant to the use of IVIg therapy in the treatment of AMBDs. Special emphasis was placed on indications for its use, determination of outcome parameters, and development of a protocol for its therapeutic use. We also focused on its safety and on prevention of adverse effects. CONCLUSION This consensus statement outlines the scope of IVIg treatment; provides guidelines for its use, including indications, prescreening, premedications, dose, frequency, and monitoring; and defines the end point of therapy.
Intergenerational Welfare Use among Immigrants: Myth of Culture of Poverty and Welfare Dependency

Secondary data and logistic regression were used to analyze risk factors in welfare use by immigrant parents and, over time, their adult children. The data described 2,087 parent–child pairs and came from the Children of Immigrants Longitudinal Study. Results showed a link between parent’s receipt of factors: current Medicaid and food stamps use; receipt of nongovernmental aid on arrival in the United States; low income; unemployed status; unmarried status; minority ethnicity other than Black. Results for the adult children of the studied immigrants showed the children’s use of Temporary Assistance for Needy Families to be associated with low income, unemployed status, number of dependent children, and White race/ethnicity. It was not associated with parental factors, including parent’s welfare use. Parents’ and children’s welfare use were not associated with citizenship status and length of residence in U.S. This study concluded that restrictive policies against immigrants’ TANF receipt should be re-evaluated.