Miracle-dz/UniFAR-FTUs huggingface dataset:
Application of BMP-bone cement and FGF-gel on periodontal tissue regeneration in non-human primates.

The ultimate challenge of tissue engineering research is the translation of experimental knowledge into clinical application. In the pre-clinical testing phase of any new therapy, animal models remain the gold standard. Therefore, the methodological choice of a suitable model is critical to meet the requirements for a safe clinical application of the developed treatment. For instance, we have shown in rats that the application of calcium phosphate cement (CPC)/propylene glycol alginate (PGA) with bone morphogenetic protein (BMP)-2 or fibroblast growth factor (FGF)-2 resulted in the regeneration of periodontal defects. However, it is debated whether using small models form a predictive method for translation to larger species. At the same time, the 3R framework is encouraged as the guiding principles of the ethical use of animal testing. Therefore, based on the successful rat study, the objective of this study was to further investigate the periodontal regenerative efficacy of the CPC/BMP and PGA/FGF system in a periodontal defect model with a low number of non-human primates (NHPs). Three Macaca fascicularis - overstocked from breeding for other purposes - were used (re-use of animals and appropriateness of the experimental animal species according to 3R framework). Three-wall periodontal defects were surgically created in the mandible. In total, 10 defects were created and distributed over two groups: 1) control group: PGA+CPC (n=5); 2) experimental group: PGA/FGF+CPC/BMP (n=5). After 3 months, tissue regeneration was evaluated by histomorphometry and radiographic measurements. Data showed that epithelial downgrowth, cementum and ligament regeneration were significantly enhanced in the experimental group compared with the control group (n=5; p=0.013, p=0.028, p=0.018, respectively). However, the amount of newly formed bone did not differ (p=0.146). Overall, as a translational proof-of-principle study, the hybrid periodontal regenerative method of CPC/BMP+PGA/FGF promoted periodontal regeneration in NHPs. This study warrants the application of CPC/BMP/PGA/FGF in clinical trials.
Insulin resistance impairs response to doxazosin therapy in men with benign prostatic hyperplasia

To evaluate the effects of insulin resistance (IR) on outcomes of doxazosin treatment for lower urinary tract symptoms (LUTS) due to benign prostatic hyperplasia (BPH). Materials and methods: This prospective study included 64 patients with LUTS due to BPH. LUTS were evaluated using the international prostate symptom score (IPSS) and maximum flow rate (Qmax) in uroflowmetry. Diagnosis of IR was achieved using homeostasis model assessment (HOMA) score and patients were grouped with respect to absence (group 1, n = 44) or presence (group 2, n = 20) of IR. Patients were evaluated before and after 3 months of daily doxazosin therapy (4 mg extended release tablet). Results: Doxazosin significantly improved the IPSS and Qmax in group 1 (P = 0.04 and P = 0.008, respectively). In group 2, doxazosin treatment had no significant therapeutic effect on mean IPSS and Qmax (P = 0.116 and P = 0.477, respectively). On the other hand, doxazosin significantly improved mean HOMA scores of patients with IR (P = 0.015). Conclusion: IR impairs response to doxazosin treatment for LUTS due to BPH. The presence of IR should be kept in mind in patients refractory to doxazosin therapy.
Interrater reliability, concurrent validity, and predictive validity of the risk for sexual violence protocol.

OBJECTIVE This study empirically evaluated risk judgments made using the Risk for Sexual Violence Protocol (RSVP; Hart et al., 2003), a widely used set of structured professional guidelines for assessing and managing sexual violence risk. HYPOTHESIS Based on evaluations of other structured professional judgment guidelines, we hypothesized that judgments made using the RSVP would demonstrate good interrater reliability, concurrent validity, and predictive validity. METHOD Based on file review, research assistants made ratings using the RSVP and two commonly used actuarial tools for sexual violence risk assessment in a sample of 100 adult male sexual offenders who successfully completed a community-based sex offender treatment program. Recidivism information was obtained from official records 10 years after treatment completion. RESULTS With respect to interrater reliability, judgments of the presence and relevance of individual risk factors ranged from moderate to almost perfect, and those for composite scores reflecting the sum of these ratings were almost perfect. Interrater reliability for integrative summary risk ratings was substantial to almost perfect. Regarding concurrent validity, the findings indicated that judgments made using the RSVP had moderate to large and statistically significant correlations with scores on the actuarial tools. Finally, with respect to predictive validity, RSVP presence total scores and summary risk ratings were predictive of new sexual violence over the follow-up, and the magnitude of their predictive validity effect sizes was similar to that of scores on the actuarial tools. CONCLUSIONS The findings supported the potential utility of the RSVP in practice. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
The Remarkable Effect of Potassium Iodide in Eosin and Rose Bengal Photodynamic Action against Salmonella Typhimurium and Staphylococcus aureus

Antimicrobial photodynamic therapy (aPDT) has been shown as a promising technique to inactivate foodborne bacteria, without inducing the development of bacterial resistance. Knowing that addition of inorganic salts, such as potassium iodide (KI), can modulate the photodynamic action of the photosensitizer (PS), we report in this study the antimicrobial effect of eosin (EOS) and rose bengal (RB) combined with KI against Salmonella enterica serovar Typhimurium and Staphylococcus aureus. Additionally, the possible development of bacterial resistance after this combined aPDT protocol was evaluated. The combination of EOS or RB, at all tested concentrations, with KI at 100 mM, was able to efficiently inactivate S. Typhimurium and S. aureus. This combined approach allows a reduction in the PS concentration up to 1000 times, even against one of the most common foodborne pathogenics, S. Typhimurium, a gram-negative bacterium which is not so prone to inactivation with xanthene dyes when used alone. The photoinactivation of S. Typhimurium and S. aureus by both xanthenes with KI did not induce the development of resistance. The low price of the xanthene dyes, the non-toxic nature of KI, and the possibility of reducing the PS concentration show that this technology has potential to be easily transposed to the food industry.
Initial clinical radiological findings and staging to predict prognosis of primary hepatic angiosarcoma: A retrospective analysis

Objective Primary hepatic angiosarcoma (PHA) is extremely rare and most patients die within 12 months of diagnosis. The object of the study is to determine the association of initial clinical-radiological features and staging with outcomes in patients with PHA. Methods The medical records of adult patients with PHA were retrieved from an electronic medical record database and a pathology database and retrospectively reviewed. During 10 years, 22 eligible patients were included. Data extracted focused on the information before the first formal treatment with a pathological proof, including demographic characteristics, medical history, laboratory data, preliminary images, histopathological records, treatment, and follow-up survival period. Two radiologists blindly re-analyzed preliminary images of all 22 patients together and recorded tumor features and imaging stage based on the American Joint Committee on Cancer (AJCC) 8th edition tumor-node-metastasis (TNM) Staging System for hepatocellular carcinoma. A radiologist compiled the initial clinical data and preliminary image stage to analyze the association with patients’ survival outcome. Results Higher aspartate aminotransferase (AST), higher total bilirubin (TB), lower albumin (ALB), longer prothrombin time (PT) and lower platelet count of serum relative to the normal reference range were more common in patients who survived ≤ 90 days (all P < 0.05). Overall survival was much better in patients with single PHA than in those with other tumor patterns of multiple PHA (all P < 0.05). Overall survival determined by preliminary imaging showed significant differences between stage I and stage III (P = 0.044), stage I and stage IV (P = 0.011), and stage III and IV (P = 0.047). No patients were at stage II. Conclusions Initial serum levels of ALT, TB, ALB, and PT, platelet count, single mass in liver, and preliminary imaging staging could help predict survival outcomes of patients with PHA.
CYLD, a Mechanosensitive Deubiquitinase, Regulates TGFβ Signaling in Load-Induced Bone Formation

Many signaling pathways involved in bone homeostasis also participate in the anabolic response of bone to mechanical loading. For example, TGFβ signaling coordinates the maintenance of bone mass and bone quality through its effects on osteoblasts, osteoclasts, and osteocytes. TGFβ signaling is also essential for the mechanosensitive formation of new bone. However, the mechanosensitive mechanisms controlling TGFβ signaling in osteocytes remain to be determined, particularly those that integrate TGFβ signaling with other early responses to mechanical stimulation. Here, we used an in vivo mouse hindlimb loading model to identify mechanosensitive molecules in the TGFβ pathway, and MLOY4 cells to evaluate their interactions with the prostaglandin E2 (PGE2) pathway, which is well-known for its rapid response to mechanical stimulation and its role in bone anabolism. Although mRNA levels for several TGFβ ligands, receptors, and effectors were unchanged, the level of phosphorylated Smad3 (pSmad3) was reduced in tibial bone as early as 3 hrs after early mechanical stimulation. We found that PGE2 and its receptor, EP2, repress pSmad3 levels and transactivation of Serpine1 in osteocytes. PGE2 and EP2 control the level of pSmad3 through a proteasome-dependent mechanism that relies on the deubiquitinase CYLD. CYLD protein levels were also reduced in the tibiae within 3 hrs of mechanical loading. Using CYLD-deficient mice, we found that CYLD is required for the rapid load-mediated repression of pSmad3 and for load-induced bone formation. These data introduce CYLD as a mechanosensitive deubiquitinase that participates in the PGE2-dependent repression of TGFβ signaling in osteocytes.
Depression, perceived social control, and hypothalamic-pituitary-adrenal axis function in African-American adults.

OBJECTIVE Social determinants may negatively affect health via Hypothalamic-Pituitary-Adrenal (HPA) axis dysfunction. The potential contribution of social determinants and related factors to HPA-axis functioning is important to study among African American adults, who are more likely to experience societal inequities and health disparities relative to other racial/ethnic groups. This study examined the relationship between depressive symptoms and perceived social control on HPA-axis functioning among African American adults. METHOD Participants (N = 107; Mage = 50, 79% female) were administered measures including the Center for Epidemiologic Studies-Depression and Informal (neighborhood) Social Control. Study procedures included the provision of 6 saliva samples for cortisol analysis (at wakeup, 30- and 90-min post-wakeup, 2:00 PM, 5:00 PM, and prebedtime). The relationship between depression and social control on the functioning of the HPA-axis were simultaneously examined within a 2-level hierarchical linear model. RESULTS Variability in the Cortisol Awakening Response (CAR) was accounted for by depressive symptomatology (p = .023) and perceived social control (p = .016), whereby greater depression was associated with a blunted CAR (less awakening cortisol production) and greater perceptions of neighborhood social control with a higher CAR. CONCLUSIONS Elevated depressive symptoms and low perceptions of neighborhood social control may serve as mechanisms that help to explain within-group variability in the functioning of stress physiology among African American adults. Findings enhance understanding of how social determinants may affect African Americans' health. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
Comparison of progesterone assay by chemiluminescence or radioimmunoassay for clinical decision-making in canine reproduction

The Coat-A-Count® radioimmunoassay has been long and widely used to determine the concentration of progesterone in serum or plasma of bitches (progRIA), but was discontinued in 2014. The Immulite® 1000 LKPG1 chemiluminescence immunoassay has gained prominence since 2003 to determine the concentration of progesterone in serum of bitches, but the assay changed in 2012 (Immulite® 1000 LKPW1). This study assessed the feasibility of using Immulite® 1000 LKPW1 (progImm) to estimate the time of clinically relevant events during oestrus and compared progRIA and progImm 2 and 3 days after the first or only day of the luteinizing hormone surge (LH1). ProgImm first exceeded 5.1 nmol/L on the same day that progRIA first exceeded 6 nmol/L, a proxy for the occurrence of the LH surge, or the day before in 28 of 31 (90%) of oestrous periods. ProgImm first exceeded 13.6 nmol/L on the same day that progRIA first exceeded 16 nmol/L (a proxy for the day of ovulation) or the day before in 34 of 35 (97%) oestrous periods. ProgImm first exceeded 5.4 nmol/L on LH1 or the day before in 24 of 25 (95%) of oestrous periods. The median of progImm 2 days after LH1 was 1.2 nmol/L lower than the 10.7 nmol/L of progRIA (p = 0.001). The mean of progImm 3 days after LH1 was 2.2 nmol/L lower than the 19.0 nmol/L of progRIA (p < 0.001). In conclusion, the days on which progImm first exceeded 5.1 nmol/L, 13.6 nmol/L and 5.4 nmol/L effectively estimate the days on which progRIA reached 6 nmol/L or 16 nmol/L or LH1.
ESTIMATION OF MORTALITY AND CALCULATED YEARS OF LOST LIFE FROM ROAD TRAFFIC INJURIES

Background and purpose: Road traffic injury (RTI) is one of the major healt h problems that can threat human health. This study was done aimed to determine mortality and years of lost life (YLL) due to RTI in Kermanshah, Iran. Materials and methods: This cross-sectional study was used the Death Regis tration and Forensics Medicine census data. YLL was calculated according to Global burden of disease (GBD) 2010 guideline and age and sex composition of the population was taken from Statis tical Center for 2010. All data collected entered i nto Excel software and performed calculations. Results: The mean age of 974 subjects studied was 39.05 ± 20.7. 81.1% of dead peoplewere males. The most frequent cause of death was related to acciden t with cars (40.8%). The mortality rate of RTI in K ermanshah province was 51.3 per 100,000 populations and by se x were 82.4 in males and 19.5 cases per 100,000 in females. The years of life lost due to premature mortality o btained 38,345 (40 per 1,000) in males, 8267 in fem ales (8.7 per 1000) and 46,613 (24.5 per 1,000) in both sexes. Th e highest YLL rate in both sexes was in the age gro up 30-34 (30.3 per 1,000). Conclusion: According to the high mortality rate and YLL due t o RTI in Kermanshah province, it seems to be necessary appropriate intervention programs done to prevent and minimize road accidents.
Sphingosine 1-Phosphate (S1P)/ S1P Receptor Signaling and Mechanotransduction: Implications for Intrinsic Tissue Repair/Regeneration

Tissue damage, irrespective from the underlying etiology, destroys tissue structure and, eventually, function. In attempt to achieve a morpho-functional recover of the damaged tissue, reparative/regenerative processes start in those tissues endowed with regenerative potential, mainly mediated by activated resident stem cells. These cells reside in a specialized niche that includes different components, cells and surrounding extracellular matrix (ECM), which, reciprocally interacting with stem cells, direct their cell behavior. Evidence suggests that ECM stiffness represents an instructive signal for the activation of stem cells sensing it by various mechanosensors, able to transduce mechanical cues into gene/protein expression responses. The actin cytoskeleton network dynamic acts as key mechanotransducer of ECM signal. The identification of signaling pathways influencing stem cell mechanobiology may offer therapeutic perspectives in the regenerative medicine field. Sphingosine 1-phosphate (S1P)/S1P receptor (S1PR) signaling, acting as modulator of ECM, ECM-cytoskeleton linking proteins and cytoskeleton dynamics appears a promising candidate. This review focuses on the current knowledge on the contribution of S1P/S1PR signaling in the control of mechanotransduction in stem/progenitor cells. The potential contribution of S1P/S1PR signaling in the mechanobiology of skeletal muscle stem cells will be argued based on the intriguing findings on S1P/S1PR action in this mechanically dynamic tissue.
Prevalence, Morphological Classification, And Factors Associated With Anemia Among Pregnant Women Accessing Antenatal Clinic At Itojo Hospital, South Western Uganda

Purpose The study aimed to determine the prevalence, morphological classification, and risk factors of anemia among pregnant mothers attending antenatal clinic at Itojo hospital, Ntungamo district, southwestern Uganda. Patients and methods After obtaining an informed consent, 5mL of blood was collected from the vein of each participant for complete blood count (CBC) and peripheral film report. The CBC was performed using HumaCount 80 hematology analyzer (HUMAN Gesellschaft für Biochemica und Diagnostica mbH Max-Planck-Ring 21 65,205 Wiesbaden Germany). Peripheral blood smears were made and stained using Wright’s Romanowsky stain and examined under ×1000 magnification for morphological classification of anemia. Structured questionnaires were administered to each participant to collect information on patients’ demography and risk factors of anaemia in pregnancy. The data generated were prepared in EXCEL and later transferred to SPSS version 20 for analysis. Univariate logistic regression and multivariate logistic regression were used to evaluate the association of socio-demographic characteristics of the participants with anemia. A 95% confidence level was used and statistical significance was reached at p<0.05. Results One hundred and sixty-three participants (n=163) were recruited for the study with the median age of 25 years and range of (17 to 40 years). The overall prevalence of anemia was 12 (7.4%), the morphological classification was 1 (8.3%) normocytic normochromic anemia, 6 (50%) microcytic hypochromic anemia, and 5 (41.7%) macrocytic anemia. Spouse occupation (p=0.03), household income (p=0.04), use of insecticide-treated mosquito nets (p=0.001), history of urinary tract infection (p=0.002), use of haematinics (p≤0.001), and history of postpartum hemorrhage (p=0.03) were significantly associated with anemia in pregnancy. Conclusion Despite the reported high prevalence of anemia in pregnancy in other areas within the country, anemia prevalence was low in this study. Routine screening for anemia at all antenatal care clinics countrywide is recommended.
Evaluating psychometric determinants of willingness to adopt sexual health patient portal services among black college students: A mixed-methods approach

Abstract Objectives: To describe, using mixed-methods, perceptions of access to sexually transmitted infection test results via electronic personal health record (PHR) and correlates of willingness to adopt its use. Participants: Students at a mid-Atlantic historically black college. Methods: Focus-groups and interviews were conducted to explore themes on sexual health-related PHR-use (N = 35). Codes were operationalized into survey measures assessing beliefs in a cross-sectional sample (N = 354). Exploratory factor analysis identified latent factors among survey items. Multiple logistic regression models measured correlates of adoption willingness. Results: Three qualitative themes emerged on relative advantages, barriers, and functionality of PHRs. 57.6% of survey participants were willing to use PHRs for sexual health services. Reliable latent factors, centering on PHR convenience and functionality, were positively associated with adoption willingness. Conclusions: Data highlights interest among black college-age youth in adopting PHRs for comprehensive sexual health-related services. Adoption may be boosted with tailored designs responsive to expressed service needs.
The Management of Type 2 Diabetes Care: The Challenge Within Primary Care

type 2 diabetes in primary care poses many challenges for health professionals in preventing and managing complications. These challenges are greatly amplified in those environments with high levels of deprivation and ethnic diversity. A retrospective structured audit of patient electronic medical records, with systematic extraction of clinical data, was undertaken in three general practices in an ethnically diverse outer London primary care trust. The records of 646 patients were analysed. Patients from minority ethnic groups were significantly more likely to have poor glycaemic control (p<0.05), and a higher proportion of patients with poor control were aged under 60 (p<0.05). Asian patients showed much higher levels of overall body mass index (BMI) risk, with all Asian patients (100%, n=85) having a sub-optimal BMI compared to other patient groups (p<0.001). Younger age (50 years and under) was associated with less frequent contact (p<0.05). Patients managed solely in general practice had significantly better glycaemic control than those in shared care schemes (p<0.01). Feedback from practices found that diabetes support services were limited and, as such, influenced health professionals' ability to provide some on-going care in terms of dietary advice and insulin initiation. The audit findings suggest that there are some significant relationships between diabetes outcomes, patient characteristics and the care process. It may be possible to develop interventions suitable for metropolitan primary care and which reflect the diversity of population needs. Copyright © 2008 John Wiley & Sons. Practical Diabetes Int 2008; 25(1): 28-36