Miracle-dz/UniFAR-FTUs huggingface dataset:
Lysine-Specific Demethylase 1 Mediates AKT Activity and Promotes Epithelial-to-Mesenchymal Transition in PIK3CA-Mutant Colorectal Cancer

Activation of the epithelial-to-mesenchymal transition (EMT) program is a critical mechanism for initiating cancer progression and migration. Colorectal cancers contain many genetic and epigenetic alterations that can contribute to EMT. Mutations activating the PI3K/AKT signaling pathway are observed in >40% of patients with colorectal cancer contributing to increased invasion and metastasis. Little is known about how oncogenic signaling pathways such as PI3K/AKT synergize with chromatin modifiers to activate the EMT program. Lysine-specific demethylase 1 (LSD1) is a chromatin-modifying enzyme that is overexpressed in colorectal cancer and enhances cell migration. In this study, we determine that LSD1 expression is significantly elevated in patients with colorectal cancer with mutation of the catalytic subunit of PI3K, PIK3CA, compared with patients with colorectal cancer with WT PIK3CA. LSD1 enhances activation of the AKT kinase in colorectal cancer cells through a noncatalytic mechanism, acting as a scaffolding protein for the transcription-repressing CoREST complex. In addition, growth of PIK3CA-mutant colorectal cancer cells is uniquely dependent on LSD1. Knockdown or CRISPR knockout of LSD1 blocks AKT-mediated stabilization of the EMT-promoting transcription factor Snail and effectively blocks AKT-mediated EMT and migration. Overall, we uniquely demonstrate that LSD1 mediates AKT activation in response to growth factors and oxidative stress, and LSD1-regulated AKT activity promotes EMT-like characteristics in a subset of PIK3CA-mutant cells. Implications: Our data support the hypothesis that inhibitors targeting the CoREST complex may be clinically effective in patients with colorectal cancer harboring PIK3CA mutations.
Effects of low‐dose oxcarbazepine administration on developing cerebellum in newborn rat: A stereological study

SUMMARY Oxcarbazepine (OXC) is a widely used novel antiepileptic drug that has been available for routine prescription for 10 years. To examine low dose OXC-induced neurotoxic effects on cerebellar development, we administered 25 mg/kg OXC orally to newborn Wistar rats once a day on postnatal days 2-14. Microscopic processed cerebellar sections of the control and treated groups were examined by volumetric analysis. Volume estimations were obtained using the Cavalieri’s principle using a computerized stereological image analyzer (CAST-GRID). The total volume of the cerebellum, white matter and the various cerebellar layers (except extragranular layer) were significantly increased in the treated animals. These data may provide useful implications for the management of OXC-induced developmental neurotoxicity in children exposed to OXC during the late fetal period. Our finings suggest that women suffering from epilepsy should be given OXC carefully only at the lowest effective doses during pregnancy.
Cinnarizine and sodium valproate as the preventive agents of pediatric migraine: A randomized double-blind placebo-controlled trial

Background Few migraine preventive agents have been assessed in a pediatric population. We evaluated the safety and efficacy of cinnarizine and sodium valproate for migraine prophylaxis in children and adolescents. Methods We carried out a randomized double-blind placebo-controlled trial in the Children’s Medical Center and Sina hospital, Tehran, Iran. Eligible participants were randomly assigned in 1:1:1 ratio via interactive web response system to receive either cinnarizine, sodium valproate, or placebo. The primary endpoints were the mean change in frequency and intensity of migraine attacks from baseline to the last 4 weeks of trial. The secondary endpoint was the efficacy of each drug in the prevention of migraine. The drug was considered effective if it decreased migraine frequency by more than 50% in the double-blind phase compared with the baseline. Safety endpoint was adverse effects that were reported by children or their parents. Results A total of 158 children participated. The frequency of migraine attacks significantly reduced compared to baseline in cinnarizine (difference: −8.0; 95% confidence interval (CI): −9.3 to −6.6), sodium valproate (difference: −8.3; 95% confidence interval: −9.3 to −7.2), and placebo (difference: −4.4; 95% confidence interval: −5.4 to −3.4) arms. The decrease was statistically greater in cinnarizine (difference: −3.6; 95% confidence interval: −5.5 to −1.6) and sodium valproate (difference: −3.9; 95% confidence interval: −5.8 to −1.9) arms, compared to placebo group. Children in all groups had significant reduction in intensity of episodes compared to baseline (cinnarizine: −4.6; 95% confidence interval: −5.2 to −4.0; sodium valproate: −4.0; 95% confidence interval: −4.8 to −3.3; placebo: −2.6; 95% confidence interval: −3.4 to −1.8). The decrease was statistically greater in cinnarizine (difference: −2.0; 95% confidence interval: −3.2 to −0.8) and sodium valproate (difference: −1.5; 95% confidence interval: −2.7 to −0.3) arms, compared to the placebo group. Seventy-one percent of individuals in the cinnarizine group, 66% of cases in the sodium valproate group, and 42% of people in the placebo arm reported more than 50% reduction in episodes at the end of the trial. The odds ratio for >50% responder rate was 3.5 (98.3% confidence interval: 1.3 to 9.3) for cinnarizine versus placebo and 2.7 (98.3% confidence interval: 1.0 to 6.9) for sodium valproate versus placebo. Nine individuals reported adverse effects (three in cinnarizine, five in sodium valproate, and one in the placebo group) and one case in the sodium valproate group discontinued the therapy due to severe sedation. Conclusion Cinnarizine and sodium valproate could be useful in migraine prophylaxis in children and adolescents. Trial registration: IRCT201206306907N4.
Outcomes of “one-day trial of vaginal breech delivery of singleton pregnancy” at 37–38 weeks’ gestation at a Japanese perinatal center

Abstract Purpose In Japan, the lack of obstetricians has made it impossible for a strict 24-h delivery monitoring system for high risk deliveries such as vaginal breech delivery at many obstetric facilities. The outcomes in the trial of vaginal breech delivery of singleton pregnancy limited one day at 37–38 weeks’ gestation were examined. Materials and methods A planned induction of vaginal breech delivery at 37–38 weeks’ gestation was performed in 72 women who got consent form April 2006 to March 2016 as follows: (1) insertion of a balloon catheter into the uterine cervix filled with a sterile saline fluid of 400–500 mL, (2) intravenous administration of oxytocin after confirming of the natural falling out by itself of the balloon catheter, and (3) selection of cesarean delivery when vaginal delivery cannot be expected by evening. The perinatal outcomes of them were examined from patient charts. Results The completion rate of vaginal delivery in total was 67% (48/72). There were no cases of neonatal asphyxia or respiratory disorders. The severe perineal lacerations occurred in one case (2%). Conclusion Planned vaginal breech delivery limited one day at 37–38 weeks’ gestation seemed to be valid and safe and it can be one of the options for breech delivery in Japan.
Predation Blocking Strategies in Real Cellular Networks and its impact on Spectrum Revenue

Encouraging new service providers to participate in the competition is one of the key ways to enhance users' utility in cellular networks. However, entry-deterrence strategy employed by the incumbents turns out to be a major obstacle in achieving this goal. Fortunately, we have recently demonstrated that the government, in its capacity as the sole provider of bandwidth, can resist such predatory behaviour by choosing suitable quadratic pricings for spectrum leasing. However, this result was premised on the presumption that both entrant and incumbent deploy base stations with same density. Moreover, their operational costs were also taken to be same. Evidently, such assumptions do not align with the reality. In this article, we investigate predation blocking strategies in real cellular networks where the above presumptions might not hold true. This generalization reveals several insightful results. For example, it shows that the government cannot block predation if the entrant's base station density is below a certain threshold. Alongside, we have also examined how these predation blocking strategies influence the spectrum revenue earned by the government. Numerical results suggest that blocking of predation is likely to degrade the government's revenue for a large number of network scenario. Finally, we study how the network economics evolves with time if the predation blocking strategies are applied repeatedly. Interestingly, we observe that after a sufficiently large number of repetitions, the incumbent eventually loses an incentive to exhibit the predatory behavior.
The responsiveness of human Vδ1 γδ T cells to Borrelia burgdorferi is largely restricted to synovial-fluid cells from patients with Lyme arthritis

It has been shown that human gamma delta T cells expressing the V delta 1 T cell receptor (TCR) respond to the spirochete Borrelia burgdorferi. Lysates of 3 Borrelia genospecies triggered the proliferation of peripheral-blood mononuclear cells not only from patients with skin manifestations of Borrelia infection and from patients with Lyme arthritis but also from healthy donors. However, with the exception of 1 patient with Lyme arthritis, no selective expansion of V delta 1-expressing gamma delta T cells was induced. In contrast, synovial-fluid mononuclear cells (SFMC) from 3 of 5 patients with Lyme arthritis responded with a selective outgrowth of V delta 1 gamma delta T cells. V delta 1 gamma delta T cell lines established from SFMC coexpressed various TCR V gamma chains, although V gamma 8 was preferentially used. Thus, the responsiveness to Borrelia antigens is not a general property of V delta 1-expressing gamma delta T cells but is largely restricted to V delta 1 gamma delta T cells recruited into the inflamed tissue.
Family history of alcohol and drug use in healthcare professionals

This study sought to ascertain if family histories of alcohol (FHPA) and drug problems (FHPD) are more prevalent among certain healthcare professions than others, and if an association between FHPA or FHPD and current alcohol and drug use exists. As part of a larger mail survey during the summer of 2002, 479 (response 68.7%) dentists, nurses, pharmacists and physicians from a north‐eastern US state completed self‐report items on FHPA, FHPD, monthly alcohol and past‐year drug use. Nurses reported a higher prevalence of FHPA than other groups of healthcare professionals (HCPs). Contrary to our prediction, FHPA in nursing was not associated with drinking level. Nurses also reported significantly higher FHPD than dentists and physicians, but not pharmacists. FHPD was significantly associated with past‐year drug use among physicians. To our knowledge, this is the first study to assess quantitatively FHPA and FHPD across multiple groups of HCPs. The results of this study suggest that FHPA and FHPD differ across groups of HCPs and a significant number choose nursing as a profession. To delineate the association between nursing and family history of substance use, more research into the familial dynamics/individual differences among these individuals is necessary.
Pioneer Female Orthopedic Surgeons as Role Models.

Maud Forrester-Brown in the United Kingdom (1921), Marika Daniilidou in Greece (1932), Ruth Jackson in the United States (1932), Loris Figgins in Australia (1957), and Evalina Burger in South Africa (1993) all chose to specialize in orthopedics. Although there are dynamic female leaders in orthopedic surgery, the field continues to present obstacles to women. Role models, mentors, and exposure to the field have been lacking for women. Although improvements have occurred in the past few decades, further changes are necessary to attract, develop, and retain qualified female candidates. [Orthopedics. 20xx; XX(X):xx-xx. ].
DFT study of the antiradical properties of some aromatic compounds derived from antioxidant essential oils: C–H bond vs. O–H bond

Abstract This paper describes a theoretical comparative study of the antiradical properties of six aromatic compounds namely eugenol (EUG), safrole (SAF), myristicin (MYR), carvacrol (CAR), cinnamaldehyde (CIN), and isoeugenol (ISO) found in antioxidant essential oils. Using density functional theory (DFT) calculations, some structural characteristics such as molecular descriptors, frontier molecular orbitals, and molecular electrostatic potential have been studied. Three main antiradical mechanisms, hydrogen atom transfer (HAT), single electron transfer proton transfer (SETPT, and sequential proton loss electron transfer (SPLET) have been also investigated. In addition, the Gibbs free energies related to the reactions of the studied compounds with two reactive oxidant species (HO• and HOO•) have been computed. Throughout the study, the implicitly of polar and nonpolar solvents (water and benzene) has been taken into account. It was found that EUG, SAF, CIN, and ISO scavenge free radicals by means of a CH bond, while CAR and ISO by means of an OH bond. In the gas and benzene phases, all the studied compounds prefer to undergo HAT mechanism, while in water, SPLET is more favoured for CIN, CAR, and ISO and both HAT and SPLET are possible for EUG, SAF, and MYR. In all the studied mediums, the sequence of the antiradical potential is: MYR ≈ SAF ≈ ISO ≈ EUG > CAR > CIN. This order is in line with the available experimental results.
The factors associated with being left-behind children in China: Multilevel analysis with nationally representative data

There are 69.7 million left-behind children (LBC) in China. Using nationally representative monitoring data for migrant workers aged 15 to 59 years in China, this study sought to estimate the prevalence of LBC in each province, and to examine risk factors being left behind at both the individual and provincial level. Data on a total of 117,573 children less than 18 years of age were included in the multilevel analysis. At individual and family level, children’s gender, age, family income, migration distance, parental separation, and housing condition in host cities were associated with being LBC. The average household monthly income in the host province was significantly associated with the migrant parents’ arrangement to leave their children behind. Comprehensive supportive policies and strategies on schooling, housing for the migrant family in host cities might be beneficial to reduce the number of LBC in China.
Evaluation of Need to Pulse Oximetry Monitoring During Inhalation Sedation for Periodontal Treatments

Introduction Inhalation sedation is used for pain and anxiety control in dentistry. Hypoxia is the most important side effect which can be detected using pulse oximetry, however, there are different opinions about the use of this instrument. This study has been designed to investigate this subject. Methods and Materials In this study, 32 adult patients from periodontal surgical department were selected for their surgical treatment to be performed under inhalation sedation with 50% oxygen and 50% nitrous oxide. Chances of hypoxia occurrence in these patients during and after surgery were evaluated, using pulse oximetry. Results Average arterial blood oxygen saturation before surgery was measured to be 98.8±0.61% while it was 99.4±0.17% during the surgery. Hypoxemia was seen in one case during the surgery. Average arterial blood oxygen saturation in the first and the fifth minute after disconnection from nitrous oxide were above 97%. Discussion Hypoxia occurred in one case which can be due to patient’s malaise and movement. Inhalation sedation may be administered without the use of pulse oximetry. However, if the observed hypoxia is a sign of real hypoxemia, we conclude that pulse oximetry is necessary during relative analgesia. Due to our lack of adequate experience with inhalation sedation, it is advisable to employ pulse oximetry for this type of sedation in dentistry. Key words Inhalation Sedation, Pulse Oximetry, N2O
Occurrence and antibiotic susceptibility of Listeria monocytogenes isolated from raw and processed meat products in Amman, Jordan

A total of 270 samples of raw and processed meat products were collected at retail in Amman, Jordan and tested for the presence of Listeria spp. by conventional bacteriological methods. L. monocytogenes isolates were confirmed by PCR using L. monocytogenes virulence gene primers actA, hlyA, iap, inlA, inlB, inlC and inlJ. The overall occurrence of L. monocytogenes was 24.4% using the culture method and 21.9% using the PCR technique. L. ivanovii was the main Listeria spp. , with an overall occurrence of 27%, while L. welshimeri and L. seeligeri showed the lowest occurrence in both types of product. All confirmed the L. monocytogenes isolates were sensitive to ampicillin, gentamicin and vancomycin while 56.6, 10, 6.7 and 5% of isolates were resistant to neomycin, tetracycline, kanamycin and erythromycin, respectively. This first report on the presence of Listeria in raw and processed meats in Jordan indicates that these products may pose a risk of listeriosis.
Reprogramming of Retrotransposon Activity during Speciation of the Genus Citrus

Abstract Speciation of the genus Citrus from a common ancestor has recently been established to begin ∼8 Ma during the late Miocene, a period of major climatic alterations. Here, we report the changes in activity of Citrus LTR retrotransposons during the process of diversification that gave rise to the current Citrus species. To reach this goal, we analyzed four pure species that diverged early during Citrus speciation, three recent admixtures derived from those species and an outgroup of the Citrus clade. More than 30,000 retrotransposons were grouped in ten linages. Estimations of LTR insertion times revealed that retrotransposon activity followed a species-specific pattern of change that could be ascribed to one of three different models. In some genomes, the expected pattern of gradual transposon accumulation was suddenly arrested during the radiation of the ancestor that gave birth to the current Citrus species. The individualized analyses of retrotransposon lineages showed that in each and every species studied, not all lineages follow the general pattern of the species itself. For instance, in most of the genomes, the retrotransposon activity of elements from the SIRE lineage reached its highest level just before Citrus speciation, while for Retrofit elements, it has been steadily growing. Based on these observations, we propose that Citrus retrotransposons may respond to stressful conditions driving speciation as a part of the genetic response involved in adaptation. This proposal implies that the evolving conditions of each species interact with the internal regulatory mechanisms of the genome controlling the proliferation of mobile elements.
Comprehensive Kinetics of Hydrolysis of Organotriethoxysilanes by 29Si NMR.

The kinetics of several representative hybrid precursors were studied via 29Si NMR: three alkyl precursors, methyltriethoxysilane, ethyltriethoxysilane, and propyltriethoxysilane; as well as two unsaturated radicals, vinyltriethoxysilane and phenyltriethoxysilane. The reaction rate is related to the chemical shift of 29Si in the NMR spectra, which gives information about the electronic density of the Si atoms and the inductive effects of substituents. The concentration of the precursors decreased exponentially with time, and the intermediate products of hydrolysis and the beginning of the condensation reactions showed curves characteristic of sequential reactions, with a similar distribution of the species as a function of the fractional conversion. For all the precursors, condensation started when the most hydrolyzed species reached a maximum concentration of 0.30 M, when the precursor had run out. A prediction following the developed mathematical model fits the experimental results in line with a common pathway described by eight parameters.
Adverse Effects of Soriatane on Rats Enterocytes: Light Microscopy and Ultrastructural Studies

Soriatane (Acitretin) drug is a synthetic vitamin A analogue and the main metabolite of etretinate. It is considered to be the latest medicine prescribed to psoriasis patients. However, it is not used frequently because of its side-effect profile. Little is known about the Acitretin intestinal toxicity, so the objective of this study is evaluation of soriatane adverse events in daily practice treatment on the duodenum enterocytes of male rats. Two groups each of 10 Wistar male rats (5-6 weeks old), one of them is the treated group and the other is the control group. The drug was dissolved immediately in distilled water before administration, and given to treated rats by gastric intubation in the morning (0.2 mg/kg/day for 12 months), while the control group received only distilled water. A significant reduction in number and height of villi with increased mucosal thickness. Damage in the duodenum tissues with villi fusion, distortion flattened epithelial cells; intense lamina propria and edema were observed. The ultra-structure alterations were indicated by Golgi apparatus, rough endoplasmic reticulum and mitochondrial atrophy with cristiolysis. Proliferation of smooth endoplasmic reticulum, nuclear deformity and increased number of liposomes were also noted. It may concluded that Soriatane led to irreversible ultra- structural alterations in rat enterocytes after 12 months treatment and precautions should be taken in prescribing acitretin to patients.
Clinical effectiveness of ultrasound guided subacromial-subdeltoid bursa injection of botulinum toxin type A in hemiplegic shoulder pain

Abstract Hemiplegic shoulder pain (HSP), which occurs in most patients with hemiplegia, causes considerable distress and worsens outcomes in rehabilitation. Although they have received the treatments such as anti-inflammatory drugs or physical therapy, many of the individuals remain suffering from shoulder pain 6 months after acute stroke event. In this retrospective study, we evaluated the effectiveness of ultrasound guided subacromial-subdeltoid (SASD) bursa injections with botulinum toxin type A (BoNT/A) compared to steroids for refractory HSP. The data were collected retrospectively by reviewing the patient's medical records and pain questionnaires in our rehabilitation center. In total, 38 patients who received ultrasound guided SASD bursa injection (BoNT/A group, n = 18; corticosteroid group, n = 20) were included. The pain visual analog scale (VAS) score at rest and during arm passive abduction, Fugl-Meyer score of upper limbs (F-M score) were evaluated before, 2, 4, 8, and 12 weeks after injection. Both 2 groups obtained a significant improvement of VAS score at rest or during arms passive abduction compared to baseline score (within group compare, P < .05). There were no significant differences of pain score improvement between two groups at week 2, 4, 8, and 12 after injection either at rest or during passive arm abduction (between 2 groups compare, P > .05). There were also no differences in results of the post treatment F-M score between 2 groups (between 2 groups compare, P > .05). Similarly, during the follow-up period no collateral effects were reported after BoNT/A injection. SASD bursa BoNT/A injection can substantially reduce the pain as corticosteroid in patients with HSP. BoNT/A injection could be a useful strategy for replacing steroids as a treatment for refractory HSP especially in the patients who cannot tolerate the steroids injection.