Miracle-dz/UniFAR-FTUs huggingface dataset:
Physical activity, total and regional obesity: dose-response considerations.

PURPOSE This review was undertaken to determine whether exercise-induced weight loss was associated with corresponding reductions in total, abdominal, and visceral fat in a dose-response manner. METHODS A literature search (MEDLINE, 1966--2000) was performed using appropriate keywords to identify studies that consider the influence of exercise-induced weight loss on total and/or abdominal fat. The reference lists of those studies identified were cross-referenced for additional studies. RESULTS Total fat. Review of available evidence suggested that studies evaluating the utility of physical activity as a means of obesity reduction could be subdivided into two categories based on study duration. Short-term studies (< or = 16 wk, N = 20) were characterized by exercise programs that increased energy expenditure by values double (2200 vs 1100 kcal.wk-1) that of long-term studies (> or = 26 wk, N = 11). Accordingly, short-term studies report reductions in body weight (-0.18 vs -0.06 kg x wk(-1)) and total fat (-0.21 vs -0.06 kg x wk(-1)) that are threefold higher than those reported in long-term studies. Moreover, with respect to dose-response issues, the evidence from short-term studies suggest that exercise-induced weight loss is positively related to reductions in total fat in a dose-response manner. No such relationship was observed when the results from long-term studies were examined. Abdominal fat. Limited evidence suggests that exercise-induced weight loss is associated with reductions in abdominal obesity as measured by waist circumference or imaging methods; however, at present there is insufficient evidence to determine a dose-response relationship between physical activity, and abdominal or visceral fat. CONCLUSION In response to well-controlled, short-term trials, increasing physical activity expressed as energy expended per week is positively related to reductions in total adiposity in a dose-response manner. Although physical activity is associated with reduction in abdominal and visceral fat, there is insufficient evidence to determine a dose-response relationship.
Setting firmer constraints on the evolution of the most massive, central galaxies from their local abundances and ages

There is still much debate surrounding how the most massive, central galaxies in the local universe have assembled their stellar mass, especially the relative roles of in situ growth versus later accretion via mergers. In this paper, we set firmer constraints on the evolutionary pathways of the most massive central galaxies by making use of empirical estimates on their abundances and stellar ages. The most recent abundance matching and direct measurements strongly favour that a substantial fraction of massive galaxies with Mstar>3 × 1011? M? reside at the centre of clusters with mass Mhalo>3 × 1013? M? Spectral analysis supports ages >10 Gyr, corresponding to a formation redshift zform>2. We combine these two pieces of observationally based evidence with the mass accretion history of their host dark matter haloes. We find that in these massive haloes, the stellar mass locked up in the central galaxy is comparable to, if not greater than, the total baryonic mass at zform. These findings indicate that either only a relatively minor fraction of their present-day stellar mass was formed in situ at zform, or that these massive, central galaxies form in the extreme scenario where almost all of the baryons in the progenitor halo are converted into stars. Interestingly, the latter scenario would not allow for any substantial size growth since the galaxy's formation epoch either via mergers or expansion. We show our results hold irrespective of systematic uncertainties in stellar mass, abundances, galaxy merger rates, stellar initial mass function, star formation rate and dark matter accretion histories.
Assessment of Patient and Caregiver Experiences of Dementia-Related Symptoms: Development of the Multidimensional Assessment of Neurodegenerative Symptoms Questionnaire

Background: To provide preliminary validation data on a self- or informant-report multidimensional questionnaire of symptoms associated with neurodegenerative disorders. Methods: Participants from 2 trials (n = 125), the Arizona APOE Cohort and the Arizona Alzheimer’s Disease Center, completed the Multidimensional Assessment of Neurodegenerative Symptoms questionnaire (MANS) and other related measures. Results: Measures of central tendency are provided for the sample as a whole and by cognitive status. Internal consistency of the MANS is excellent (α = 0.98). Factor analysis suggests 4 factors. Correlational analyses support the construct validity of the MANS with moderate to high (r = 0.54–0.87) correlations between the MANS and measures of mood, cognition and daily functioning. Conclusion: Results provide initial support for the MANS as a brief measure that is a reliable and valid indicator of cognitive, personality, functional and motor symptoms potentially related to neurodegenerative etiologies. Further research with the MANS is warranted.
Activation of Mitochondrial ATP-Sensitive Potassium Channel Contributes to Protective Effect in Prolonged Myocardial Preservation

To find a better strategy for effective donor organ preservation, here we used a model of long-term hypothermia preservation of rat hearts to investigate the cardioprotective effects of diazoxide, a selective opener of mitochondrial ATP-sensitive potassium channel (mitoKATP ). Cardiac function was impaired by 8-hour ischemic preservation following 30 minutes of reperfusion. Treatment with diazoxide significantly attenuated the decline of myocardial contractility, and decreased the lactate dehydrogenase leakage and myocardial edema. Diazoxide also prevented the loss of activity of mitochondrial superoxide dismutases and sarcolemmal Na+/K+ ATPase during ischemia or reperfusion period, respectively. These effects of diazoxide could be abolished by a selective mitoKATp blocker 5-hydroxydecanoate. The results suggest that diazoxide, as a supplementation in cardioplegic solution, could enhance myocardial protection by opening mitoKATp channel, and better maintenance of mitochondrial anti-oxidative enzyme
Performance of three glomerular filtration rate estimation equations in a population of sub‐Saharan Africans with Type 2 diabetes

We evaluated the performance of the Modification of Diet in Renal Disease (MDRD), Chronic Kidney Disease Epidemiology Collaboration (CKD‐EPI) and Cockcroft–Gault (CG) equations against creatinine clearance (CrCl) to estimate glomerular filtration rate (GFR) in 51 patients with Type 2 diabetes.
Characterization of Three Alboaggregins Purified from Trimeresurus albolabris Venom

Summary Alboaggregins (AL-A, AL-B, AL-C) isolated from Trimeresurus albolabris snake venom represent a new family of proteins which bind to platelet glycoprotein lb (GPIb). These alboaggregins were purified to homogeneity with ion exchange HPLC (Mono-Q column) and hydrophobic HPLC (TSK Phenyl-5PW column). On SDS-polyacrylamide gel electrophoresis, apparent molecular weights of AL-A, AL-B and AL-C were 52 kDa, 26 kDa, and 121 kDa respectively, under nonreducing conditions. Upon reduction, each alboaggregin showed two types of chains with apparent molecular weights in the range of 15-20 kDa. All three alboaggregins agglutinated formalin-fixed platelets. Agglutination activities and binding of labeled alboaggregins to GPIb were specifically inhibited by the monoclonal antibody AK2 which is directed against the 45 kDa N-terminal region on GPIb, but not by monoclonal antibodies against other epitopes on GPIb. 125I-alboaggregin binding to platelets was not altered by the presence of thrombin. Alboaggregins did not bind to GPIIb/IIIa. Alboaggregins were competitive inhibitors for 125I-bovine vWF binding to platelets. Mutual competition studies between AL-A, AL-B and AL-C for the binding of labeled bovine vWF and AL-B to platelets demonstrated that AL-B and AL-C had a significantly higher affinity than AL-A.
Gender of the expresser moderates the effect of emotional faces on the startle reflex

It has been suggested that the impact of emotional expressions on the startle reflex is influenced by the intention communicated by the expression (e.g., the intention to attack). However, we propose that the meaning of an emotional expression is not only based on the intention, but is also influenced by characteristics of the expresser such as gender: since men are typically seen as more dominant than women, anger expressed by men should be perceived as particularly threatening, thus amplifying the startle response. We compared the influence of anger, fear and neutral expressions shown by men and women on the startle reaction. Startle reactions were measured using electromyography. As predicted, we found stronger startle reactions after the presentation of anger expressed by men compared to fearful and neutral expressions shown by men. For female expressers, the startle response was not affected by expression type.
Characterization and Functional Complementation of a Nonlethal Deletion in the Chromosome of a β-Glycosidase Mutant of Sulfolobus solfataricus

ABSTRACT LacS− mutants of Sulfolobus solfataricus defective in β-glycosidase activity were isolated in order to explore genomic instability and exploit novel strategies for transformation and complementation. One of the mutants showed a stable phenotype with no reversion; analysis of its chromosome revealed the total absence of the β-glycosidase gene (lacS). Fine mapping performed in comparison to the genomic sequence of S. solfataricus P2 indicated an extended deletion of ∼13 kb. The sequence analysis also revealed that this chromosomal rearrangement was a nonconservative transposition event driven by the mobile insertion sequence element ISC1058. In order to complement the LacS− phenotype, an expression vector was constructed by inserting the lacS coding sequence with its 5′ and 3′ flanking regions into the pEXSs plasmid. Since no transformant could be recovered by selection on lactose as the sole nutrient, another plasmid construct containing a larger genomic fragment was tested for complementation; this region also comprised the lacTr (lactose transporter) gene encoding a putative membrane protein homologous to the major facilitator superfamily. Cells transformed with both genes were able to form colonies on lactose plates and to be stained with the β-glycosidase chromogenic substrate X-Gal (5-bromo-4-chloro-3-indoyl-β-d-galactopyranoside).
Phase gradient mapping as an aid in the analysis of object-induced and system-related phase perturbations in MRI

In this note we wish to demonstrate the utility of phase gradient mapping (PGM) as an aid in the analysis and characterization of object-induced and system-related macroscopic phase perturbations in MRI. To achieve this goal, phase gradient maps and, if applicable, field gradient maps were derived from standard phase images via a forward difference operator taking into account phase wraps. By way of phantom experiments, PGM was shown to provide reliable phase and field gradient information, even in regions with multiple phase wraps. Phase gradient mapping was further shown to allow positive identification of local phase and field perturbations and global discrimination between positive and negative local susceptibility deviations. The suitability of PGM for in vivo studies was demonstrated by a 3D brain examination of a healthy volunteer.
Age as a risk factor for burn injury requiring hospitalization during early childhood.

OBJECTIVE To examine the variation by age in the rates and causes of burn injury requiring hospitalization during early childhood. DESIGN AND SETTING Hospital discharge data and a burn unit admission log were used to identify all children in the Denver (Colo) metropolitan area younger than 5 years who sustained burn injuries and were hospitalized in 1989 and 1990. Patients' medical records were reviewed. RESULTS One hundred twenty-two children were identified with burn injuries that required hospitalization, an annual incidence of 40.5 per 100,000 children younger than 5 years. Children aged 6 months through 2 years accounted for 88% of all cases and were seven times more likely to be hospitalized for a burn injury than were children outside this age range. Scalding and contact with hot objects accounted for 64% and 20% of cases, respectively, and occurred primarily in the 6-month through 2-year age group. CONCLUSIONS The findings underscore the importance of developmental stage as a determinant of risk and type of burn injury. Children aged 6 months through 2 years are at increased risk of severe burn injury and should be targeted for prevention efforts.
Post-acute care and ACOs - who will be accountable?

OBJECTIVE To determine how the inclusion of post-acute evaluation and management (E&M) services as primary care affects assignment of Medicare beneficiaries to accountable care organizations (ACOs). DATA SOURCES Medicare claims for a random 5 percent sample of 2009 Medicare beneficiaries linked to American Medical Association Group Practice data identifying provider groups sufficiently large to be eligible for ACO program participation. STUDY DESIGN We calculated the fraction of community-dwelling beneficiaries whose assignment shifted, as a consequence of including post-acute E&M services, from the group providing their outpatient primary care to a different group providing their inpatient post-acute care. PRINCIPAL FINDINGS Assignment shifts occurred for 27.6 percent of 25,992 community-dwelling beneficiaries with at least one post-acute skilled nursing facility stay, and they were more common for those incurring higher Medicare spending. Those whose assignment shifted constituted only 1.3 percent of all community-dwelling beneficiaries cared for by large ACO-eligible organizations (n = 535,138), but they accounted for 8.4 percent of total Medicare spending for this population. CONCLUSIONS Under current Medicare assignment rules, ACOs may not be accountable for an influential group of post-acute patients, suggesting missed opportunities to improve care coordination and reduce inappropriate readmissions.
Effects of platelet-rich and -poor plasma on the reparative response of gingival fibroblasts.

OBJECTIVES Although platelet-rich plasma (PRP) has been proposed as a therapeutic tool to enhance wound repair, the cellular and molecular mechanisms stimulated by this agent are still not completely understood. The present study was designed to characterize the effects of PRP and platelet-poor plasma (PPP) supernatants on cell responses involved in gingival tissue repair. METHODS We studied the response of human gingival fibroblasts (HGF) to PRP and PPP fractions on: matrix contraction, cell migration, myofibroblastic differentiation, production of matrix components and proteolytic enzymes. PRP and PPP were obtained from donors using a commercial kit. Matrix contraction was evaluated by means of collagen lattices in the presence of matrix metalloproteinase (MMP) and actin polymerization inhibitors. The production of matrix molecules and proteinases was assessed through Western-blot. RhoA activity was evaluated through a pull-down assay. Actin distribution and focal adhesions were assessed through immunofluorescence. Transforming growth factor-beta (TGF-β) was quantified through ELISA. RESULTS Both PRP and PPP stimulated human gingival fibroblasts-populated collagen gel contraction and Ilomastat and cytochalasin D inhibited this response. PRP and PPP also stimulated MT1-MMP and TIMP-2 production, RhoA activation and actin cytoskeleton remodeling, cell migration/invasion and myofibroblastic differentiation. TGF-β1 was found at significantly higher concentrations in PRP than in PPP. CONCLUSIONS Both PRP and PPP promote wound tissue remodeling and contraction through the stimulation of actin remodeling, the activity of MMPs, promotion of cell migration, and myofibroblastic differentiation. The similar biological responses induced by PRP and PPP suggest that both platelet-derived fractions may exert a positive effect on gingival repair.
Cerebral glucose metabolism in patients with AD and different APOE genotypes

Objective: To examine the influence of the APOE ε4 allele on cerebral glucose metabolism in a large series of patients with Alzheimer disease (AD). Methods: Eighty-three patients (41 APOE ε4 positive and 42 ε4 negative) were selected from a pre-existing databank of patients with AD (n > 1,000). The patients were carefully matched for age, age at onset, approximate disease duration, educational level, and overall degree of cognitive impairment. Cerebral [18F]fluorodeoxyglucose PET imaging was performed in all patients by a standardized protocol. Statistical comparison of patient PET data vs a healthy control population was performed as well as an analysis of differences between groups (SPM99; Wellcome Department of Cognitive Imaging, London, UK). Results: A similar pattern of cerebral hypometabolism was detected in the ε4-positive and -negative patient groups vs healthy volunteers in regions typically affected by AD (bilateral temporal, parietal, posterior cingulate, and prefrontal cortical areas). The comparison between ε4-positive and -negative patients additionally revealed stronger abnormalities in ε4 carriers in parietal, temporal, and posterior cingulate cortical regions. Conclusions: A generally similar pattern of cerebral hypometabolism was detected in APOE ε4-positive and -negative patients with Alzheimer disease. However, in direct comparison of the two matched groups, the abnormalities in the ε4-positive group were demonstrated to be more pronounced.
Enterobacter hormaechei subsp. oharae subsp. nov., E. hormaechei subsp. hormaechei comb. nov., and E. hormaechei subsp. steigerwaltii subsp. nov., Three New Subspecies of Clinical Importance

ABSTRACT Six species and six additional genovars are combined within the so-called Enterobacter cloacae complex, with one of them being the species Enterobacter hormaechei. In a recent population genetic study, two genetic clusters were found in close phylogenetic proximity to the genetic cluster of E. hormaechei. In order to prove the hypothesis that these three genetic clusters belong to the same species, we performed cross-hybridization experiments in microplates with DNAs of representatives of each genetic cluster. The close phylogenetic relationship among the clusters was reflected by their relatively low ΔTm values, ranging from 0.3 to 4.8, confirming the hypothesis that the clusters are parts of the same species. These clusters can be distinguished from the other species of the E. cloacae complex, which have ΔTm values of 5.6 to 10.3. Forty-eight E. hormaechei strains from the different genetic clusters were phenotypically characterized with 129 biochemical tests. In this way, E. hormaechei could be differentiated from the other species of the E. cloacae complex because it tests negative in the 3-hydroxy-butyrate test. The three genetic clusters of E. hormaechei could also be differentiated from each other by using phenotypic tests. Hence, we propose three new subspecies of E. hormaechei corresponding to genetic clusters VI, VII, and VIII of the E. cloacae complex. E. hormaechei subsp. hormaechei comb. nov. corresponds to the original species description, as it gives negative results for the adonitol, d-arabitol, d-sorbitol, and d-melibiose tests and a positive result for the dulcitol test. E. hormaechei subsp. oharae subsp. nov. gives negative results for the dulcitol, adonitol, and d-arabitol tests and positive results for the d-sorbitol and d-melibiose tests. E. hormaechei subsp. steigerwaltii subsp. nov. gives a negative result for the dulcitol test and positive results for the adonitol, d-arabitol, d-sorbitol, and d-melibiose tests. Among the members of the E. cloacae complex, E. hormaechei seems to be the species most frequently recovered from clinical specimens.
Performance-based physical function in older community-dwelling persons: a systematic review of instruments.

BACKGROUND Identification of older persons at risk for the loss of independence, onset of (co)-morbidity or functional limitations through screening/assessment is of interest for the public health-care system. To date several different measurement instruments for overall physical function are frequently used in practice, but little information about their psychometric properties is available. Objectives and METHODS Our aim was to assess instruments with an overall score related to functional status and/or physical performance on content and psychometric properties. Electronic databases (Medline, EMBASE, AMED, Cochrane Library and CINAHL) were searched, using MeSH terms and relevant keywords. Studies, published in English, were included if their primary or secondary purpose was to evaluate the measurement properties of measurement instruments for overall physical function in community-dwelling older persons aged 60 years and older. Reliability, validity, responsiveness and practicability were evaluated, adhering to a specified protocol. RESULTS In total 78 articles describing 12 different functional assessment instruments were included and data extracted. Seven instruments, including their modified versions, were evaluated for reliability. Nine instruments, including their modified versions, were evaluated with regard to validity. CONCLUSION In conclusion, the Short Physical Performance Battery can be recommended most highly in terms of validity, reliability and responsiveness, followed by the Physical Performance Test and Continuous Scale Physical Functional Performance.
A twin study of genetic and dietary influences on nephrolithiasis: a report from the Vietnam Era Twin (VET) Registry.

BACKGROUND Nephrolithiasis is a complex phenotype that is influenced by both genetic and environmental factors. We conducted a large twin study to examine genetic and nongenetic factors associated with stones. METHODS The VET Registry includes approximately 7500 male-male twin pairs born between 1939 to 1955 with both twins having served in the military from 1965 to 1975. In 1990, a mail and telephone health survey was sent to 11,959 VET Registry members; 8870 (74.2%) provided responses. The survey included a question asking if the individual had ever been told of having a kidney stone by a physician. Detailed dietary habits were elicited. In a classic twin study analysis, we compared concordance rates in monozygotic (MZ) and dizygotic (DZ) twins. We also conducted a cotwin control study of dietary risk factors in twins discordant for stones. RESULTS Among dizygotic twins, there were 17 concordant pairs and 162 discordant pairs for kidney stones. Among monozygotic twins, there were 39 concordant pairs and 163 discordant pairs. The proband concordance rate in MZ twins (32.4%) was significantly greater than the rate in DZ twins (17.3%) (chi(2)= 12.8; P < 0.001), consistent with a genetic influence. The heritability of the risk for stones was 56%. In the multivariate analysis of twin pairs discordant for kidney stones, we found a protective dose-response pattern of coffee drinking (P= 0.03); those who drank 5 or more cups of coffee were half as likely to develop kidney stones as those who did not drink coffee (OR = 0.4, 95% CI 0.2, 0.9). Those who drank at least 1 cup of milk per day were half as likely to report kidney stones (OR = 0.5, 95% CI 0.3, 0.8). There were also marginally significant protective effects of increasing numbers of cups of tea per day and frequent consumption of fruits and vegetables. Other factors such as the use of calcium supplements, alcohol drinking, consumption of solid dairy products, and the amount of animal protein consumed were not significantly related to kidney stones in the multivariate model. CONCLUSION These results confirm that nephrolithiasis is at least in part a heritable disease. Coffee, and perhaps tea, fruits, and vegetables were found to be protective for stone disease. This is the first twin study of kidney stones, and represents a new approach to elucidating the relative roles of genetic and environmental factors associated with stone formation.