Miracle-dz/UniFAR-FTUs huggingface dataset:
Value of high‐risk HPV‐DNA testing in the triage of ASCUS

Objective. Atypical squamous cells of undetermined significance (ASCUS) cells, occurring in organized cytological screening, may be either high‐risk human papillomavirus (HPV) positive or negative. To refine the assessment of women with ASCUS, a high‐risk HPV‐DNA test is recommended as triage in Sweden. Methods. A total of 197 consecutive women (mean age 39 years, range 21–60) with a diagnosis of ASCUS from the primary screening were selected for triage. Their cervical smears were collected and evaluated by using conventional cytological examination in combination with a high‐risk HPV‐DNA test (hybrid capture 2). The women were categorized into four groups: Group A, Cytology + /HPV +; Group B, Cytology–/HPV +; Group C, Cytology + /HPV–; and Group D, Cytology–/ HPV–. Women within Groups A–C were admitted for colposcopy and cervical biopsy. The women in Group D were considered as a low‐risk group for tumor development, and were re‐examined after three years in the next round of the organized screening. Results. In women in Group A (n = 58) the prevalence of histological verified CIN2–3 was 41%, in Group B (n = 41) 20%, and in Group C (n = 9) 0%. In Group D (n = 89), repeated primary screening three years later revealed CIN2–3 in two biopsies from 74 women studied (<3%). The prevalence of a high‐risk HPV infection decreased with age in women with ASCUS. It was 74% in women <30 years and 19% in women ≥50 years. Conclusions. Adding a high‐risk HPV test in secondary screening increased the identification of women with CIN2–3 lesions by 33% in comparison with repeat cytology (p = 0.01). The clinical significance of the ASCUS diagnosis varied with age of the women.
Difference in mechanical and energy cost between highly, well, and nontrained runners.

INTRODUCTION Recently it has been shown that endurance training decreases the variability in stride rate. This decrease would lead to a reduction in the mechanical and the energy cost of running. PURPOSE This study therefore aimed to compare the mechanical and the energy cost of running according to the training status of the runner (highly, well, and nontrained endurance runners). METHODS The kinetic, potential, and internal mechanical costs (Cke, Cpe, and Cint) were measured with a 3D motion analysis system (ANIMAN3D). The energy cost of running (C) was measured from pulmonary gas exchange using a breath-by-breath portable gas analyser (Cosmed K4b2, Rome, Italy). All the parameters were measured on track, for a speed of 4.84 +/- 0.36 m x s(-1). RESULTS Highly trained runners did not exhibit significantly lower C compared with well or nontrained runners (4.46 +/- 0.38; 4.33 +/- 0.32; 4.46 +/- 0.46 J x kg(-1) x m(-1), respectively; P = 0.75). However, Cpe was significantly lower in highly and well-trained runners compared with nontrained runners (0.43 +/- 0.07; 0.45 +/- 0.05; 0.54 +/- 0.08 J x kg(-1) x m(-1), respectively; P < 0.05). In contrast, Cint was significantly higher in highly trained runners compared with well and nontrained runners (respectively, 0.80 +/- 0.12; 0.60 +/- 0.09; 0.59 +/- 0.10 J x kg(-1) x m(-1); P < 0.05). CONCLUSION Although there is a significant difference in Cpe and in Cint between runners of various training status, there is no difference in C. Differences in Cpe and Cint may be associated with the same self-optimizing mechanism that contributes to a reduction in the impact loads during the initial portion of the support phase of the stride.
Optimizing the treatment of rhegmatogenous retinal detachment

Surgical approaches for rhegmatogenous retinal detachment (RRD) have evolved rapidly in the past century. This has resulted in an increase in the anatomical success rate from zero per cent in the beginning of the 1900s to now almost 100 per cent. Rhegmatogenous retinal detachment is regarded as an acute eye disease that needs immediate treatment. With the increasing number of cataract surgeries and an increased elderly population, the numbers of RRD occurrences are increasing. The aim of this thesis is to create knowledge on how treatment and care of RRD patients can be optimized. In the first paper, data on the incidence of RRD in Denmark are presented based on data from a nation register the National Patient Registry (NPR). It was discovered that the incidence of RRD in Denmark is similar to previous reported numbers and that the incidence has been increasing due to increasing numbers of cataract surgeries and an increased elderly population. Using data from the NPR, we estimated that the risk of a RRD occurring on the fellow eye is 100 times larger than on the first eye and that middle aged men have the highest risk. Having an increase in the incidence of RRD we need to ensure that the patients are also treated in the most optimal way. To ensure this, an indicator is needed to monitor the quality at the different centres. This indicator presented in the second paper is based in the occurrence of redetachment. We define a detachment to be caused by poor surgery if the retina detaches within one year after initial surgery with pneumatic retinopexy, scleral buckling and VTX with gas, and one and a half years after surgery with VTX with oil. Also lack of oil removal within the first year is a failed operation. It is widely accepted that RRD is an acute disease but when should surgery be performed to attain the most optimal result? In the third paper, we evaluated the progression of posterior RRD with an optical coherence tomography to make an objective assessment of the movement. We found that the risk of a macula on RRD progressing to affect the fovea is small if the patient is postured appropriately. We found that the movement of the RRD is dynamic but the detachments will ultimately approach the fovea. Having an opportunity to postpone surgery without risking the outcome for the patient allows a more optimal surgery. The results of this thesis can be used by all health care systems to establish optimal conditions in the treatment of RRD.
Factor VIIa-antithrombin complex: a possible new biomarker for activated coagulation

Abstract The activation of the extrinsic coagulation pathway occurs after endothelial injury when the tissue factor (TF), a transmembrane protein located outside the vasculature, binds factor VII (FVII) or activated FVII (FVIIa). Once formed, the TF-VIIa complex activates both factor IX and X and initiates the coagulation process. The TF-VIIa complex is inhibited by both TF pathway inhibitor (TFPI) and antithrombin (AT). The interaction between TF-VIIa and AT induces FVIIa-AT complex formation, which is released into the plasma. Because AT reacts with FVIIa only when it is bound to TF, the circulating levels of FVIIa-AT reflect the degree of exposure of TF to blood. Preliminary clinical studies have shown higher plasma levels of FVIIa-AT complex both in patients with a prior arterial or venous thrombotic event. Increased plasma levels of FVIIa-AT have also been reported in a number of other prothrombotic conditions – antiphospholipid antibodies, solid and hematological malignancies, pre-eclampsia (PE), obesity and cardiac surgery. However, most of the studies published so far are retrospective and with a limited sample size. Larger prospective clinical studies are needed to confirm these findings and to assess the prognostic role of this possible new biomarker for activated coagulation.
BREEDING OF THE BROOD PARASITIC COMMON CUCKOO (CUCULUS CANORUS) IN REED HABITATS IN NW VOJVODINA, SERBIA

dinaceus) nests were found and monitored. The overall parasitism rate for the study area was 0.127, which was calculated from the number of parasitized nests (76) divided by the total number of Great Reed Warbler nests (596). The parasitism rate did not vary with the total number of Great Reed Warbler nests at a study site. The number of parasitized and successful parasitized nests showed significant negative relationship, which means more parasitized nests resulted with more brood lost. The parasitism rate varied significantly among habitats but not among years while the number of successfully parasitized nests differed among both habitats and years. Parasitized nests found in small and large canals showed the highest reproductive success of Cuckoos. Loss of parasitized nests through predation and bad weather circumstances was relatively high in this study compared to other areas.
On Improving the Performance of MI-MAC over Next Generation Wireless Cellular Networks

In recent work [1], we have introduced MI-MAC (Multimedia Integration Multiple Access Control Protocol), which was shown to achieve superior performance in comparison to other protocols of the literature when integrating various types of multimedia traffic. In this paper we enhance the scheduling scheme of MI-MAC by adding three important parameters into its study, namely handoff traffic, per user varying channel conditions in the uplink and downlink channels and video sources' contention for channel resources, in order to evaluate the protocol under a significantly more realistic wireless cellular network scenario.
Comparison of Autologous Bone Marrow Mononuclear Cells Transplantation and Mobilization by Granulocyte Colony-Stimulating Factor in Experimental Spinal Injury

ABSTRACT Background: Recent studies have shown that autologous adult stem cells may be a protocol of treatment for spinal cord injury (SCI) in humans. The purpose of this study was to compare the effect of an injection of autologous bone marrow mononuclear cells (BMMCs) and bone-marrow cell mobilization induced by granulocyte colony stimulating factor (G-CSF) in rats with SCI. Methods: Adult rats were assigned into three groups: control group (SCI only), SCI + BMMCs, and SCI + G − CSF. Neurological scores and electrophysiological testing were done in all rats before SCI, and at 1, 7, 14, 28, 56 days post-SCI. Simultaneously, immunohistochemical labeling and TUNEL assay were performed at the given time. Results: From 1 week post-SCI onward, animals treated with BMMCs or G-CSF had higher BBB scores than control group. Motor and somatosensory evoked potentials (MEPs and SEPs, respectively) of the treated group were significantly better than those in control group at 2 weeks. After sacrifice, compared with the control, animals treated with BMMCs and G-CSF significantly increased expressions of Brdu, NSE, GFAP, Factor VIII, BDNF, and reduced expression of apoptosis cells around the lesion site. Our results indicate that administration of BMMCs and G-CSF in a SCI model achieves similarly positive effect on functional and histologic recovery. Conclusions: The use of G-CSF may be a viable alternative to BMMCs for autograft in patients with SCI.
Comparative neurochemical effects of repeated methyl parathion or chlorpyrifos exposures in neonatal and adult rats.

Several studies have reported higher sensitivity based on lethality in young animals compared to adults following acute exposure to organophosphorus insecticides (OPs). We propose that age-related differences in sensitivity to OPs may differ qualitatively and quantitatively with different OPs and varying exposure conditions (e. g., high vs. low dose, acute vs. repeated). To test this hypothesis, we treated neonatal (7 days of age) and adult (90 days of age) rats with either methyl parathion (MPS) or chlorpyrifos (CPF) daily for 14 days and measured neurochemical endpoints {cholinesterase (ChE) inhibition, total muscarinic receptor ([(3)H]quinuclidinyl benzilate, QNB) and muscarinic M2 subtype-preferential ([(3)H]AF-DX 384) binding} in frontal cortex and striatum at timepoints both during (1 day after the 7(th) and 14(th) dose) and after (8 days after the 14(th) dose) exposures. Repeated CPF exposures were associated with relatively similar degrees of ChE inhibition between the age groups during dosing but more extensive inhibition was noted in adults after termination of exposures. Relatively similar changes in muscarinic receptor binding were also noted between age groups following CPF exposures. Moreover, the degree of muscarinic receptor binding reduction relative to ChE inhibition appeared similar in both age groups following CPF exposures. In contrast, ChE activity and muscarinic receptor binding were generally more reduced in neonatal relative to adult brain regions following repeated MPS exposures. Furthermore, the relationship between the degree of ChE inhibition and the reduction in cortical muscarinic receptor binding appeared different between the age groups, i.e., more extensive reduction was noted in neonates compared to adults with a given level of ChE inhibition. We conclude that OP-selective differences in in vivo ChE sensitivity, differential rates of enzyme recovery following inhibition, and age-dependent differences in muscarinic receptor adaptations can all influence the nature of age-related susceptibility to OPs.
The Expanding Workweek? Understanding Trends in Long Work Hours Among U.S. Men, 1979-2004

After declining for most of the century, the share of employed American men regularly working more than 50 hours per week began to increase around 1970. This trend has been especially pronounced among highly educated, high-wage, salaried, and older men. Using two decades of CPS data, we rule out a number of factors, including business cycles, changes in observed labor force characteristics, and changes in the level of men's real hourly earnings as primary explanations of this trend. Instead we argue that increases in salaried men's marginal incentives to supply hours beyond 40 accounted for the recent rise. Since these increases were accompanied by a rough constancy in real earnings at 40 hours, they can be interpreted as a compensated wage increase.
Blocking of L-type calcium channels protects hippocampal and nigral neurons against iron neurotoxicity The role of L-type calcium channels in iron-induced neurotoxicity

Iron plays an important role in maintaining normal brain function. However, iron overload and enhanced hydroxyl radical formation have been implicated as the causative factors of some neurodegenerative disorders such as Parkinson's and Alzheimer's diseases. Calcium is also required for diverse physiological process including secretion of neurotransmitters, synaptic plasticity, gene expression and axonal growth. Iron and calcium are essential for neuronal function but, when present in excessive level, they induce neuronal damage and may even cause neuronal death. Some reports suggest that voltage gated calcium channels (VGCCs) are an alternate route for iron entry into neuronal cell lines under conditions of iron overload. The aim of the present study was to investigate the effects of L-type VGCCs on iron-induced neurotoxicity. Iron neurotoxicity was generated by intracerebroventricular FeCl3 injection. Nicardipine treatment (10 mg/kg/d) was applied to block L-type VGCCs for 10 d. Rats were perfused intracardially under deep urethane anaesthesia after treatment period. Removed brains were processed using the standard histological techniques. The numbers of neurons in hippocampus and substantia nigra of all rats were estimated by stereological techniques. Results of present study show that nicardipine decreased hippocampal and nigral neuron loss from 43.9% to 18.4% and 41.0% to 12.1%, respectively. Outcomes of the present study propose that blocking of L-type VGCCs may reduce the neurotoxic effects of iron by inhibiting the cellular influx of excessive calcium and/or iron ions.
Mcleod syndrome: A novel mutation, predominant psychiatric manifestations, and distinct striatal imaging findings

The McLeod syndrome is an X‐linked disorder caused by mutations of the XK gene encoding the XK protein. The syndrome is characterized by absent Kx erythrocyte antigen, weak expression of Kell blood group system antigens, and acanthocytosis. In some allelic variants, elevated creatine kinase, myopathy, neurogenic muscle atrophy, and progressive chorea are found. We describe a family with a novel point mutation in the XK gene consisting of a C to T base transition at nucleotide position 977, introducing a stop codon. Among seven affected males, five manifested with psychiatric disorders such as depression, bipolar disorder, or personality disorder, but only two presented with chorea. Positron emission tomography and magnetic resonance volumetry revealed reduced striatal 2‐fluoro‐2‐deoxy‐glucose (FDG) uptake and diminished volumes of the caudate nucleus and putamen that correlated with disease duration. In contrast, none of 12 female mutation carriers showed psychiatric or movement disorders. However, a semidominant effect of the mutation was suggested by erythrocyte and blood group mosaicism and reduced striatal FDG uptake without structural abnormalities. Therefore, patients with psychiatric signs or symptoms segregating in an X‐linked trait should be examined for acanthocytosis and Kell/Kx blood group serology. Ann Neurol 2001;49:384–392
Differential recognition of viral RNA by RIG-I

Retinoic acid inducible gene I (RIG-I) is a pattern recognition receptor (PRR) responsible for detection of nucleic acids from pathogens in the cytoplasm of infected cells and induction of type I interferon (IFN). RIG-I -specific pathogen associated molecular patterns (PAMPs) are characterized by RNA molecules with a 5'-triphosphate (5'-ppp) group and partial double-stranded composition. Although many RNA molecules capable of activating RIG-I have been described, the exact nature of viral RNAs which are responsible for triggering RIG-I activity during the course of an infection has not been extensively explored and the specificity of RIG-I for various viral RNA molecules remains largely unknown. By examining endogenous RIG-I/RNA complexes in influenza virus and Sendai virus infected cells we were able to identify viral RNA molecules which specifically associated with RIG-I during infection. We showed that in Sendai virus infected cells, RIG-I specifically and preferentially associated with the copy-back defective interfering (DI) particle RNA and not with the full-length Sendai virus genome or Sendai virus encoded mRNAs. In influenza virus infected cells RIG-I also preferentially associated with DI RNAs as well as with the shorter genomic segments.
Protective effects of early treatment with propofol on endotoxin-induced acute lung injury in rats.

BACKGROUND To investigate the effects of propofol administration on acute lung injury in endotoxin-induced shock in rats. METHODS Seventy-six male Wistar rats were randomly assigned to one of five groups: (i) saline control; (ii) endotoxin alone (receiving lipopolysaccharide (LPS) 8 mg kg(-1) i.v. ); (iii) pretreatment with propofol 1 h before LPS; (iv) simultaneous treatment with propofol and LPS; (v) post-treatment with propofol 1 h after LPS. During the 5 h after LPS injection, survival rates were recorded. Lung tissue was sampled to measure values of nitrite/nitrates (NO(2-)/NO(3-)) and tumour necrosis factor (TNF)-alpha in bronchoalveolar lavage (BAL) fluid, and wet-to-dry lung weight ratio, pulmonary permeability index, BAL protein and expression of inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT). RESULTS Compared with the endotoxaemic group, both the pre- and simultaneous treatment groups showed significantly improved 5 h survival rates, and attenuated endotoxin-induced increased BAL fluid NO(2-) /NO(3-) and TNF-alpha, iNOS mRNA and NT expression in lung tissue, and decreased pulmonary microvascular permeability. These beneficial effects were blunted in the post-treatment group. CONCLUSIONS These findings indicate that early administration of propofol may provide protective effects against endotoxin-induced acute lung injury.
Cerebral Glucose Transport Implies Individualized Glial Cell Function

Previous positron emission tomography (PET) measurements of cerebral glucose transport using [11C]-3-O-methylglucose (CMG) suggested an interindividual variation in the values of the rate constant of tracer outflow (k2) larger than that for the clearance rate of inflow (K1). These two parameters were examined in healthy cerebral cortex by dynamic PET in 4 men and 2 women (aged 24 to 73 years) without neurologic disease, and in 1 man (42 years) with a recent left hemispheric cerebral infarction under normoglycemia (average blood plasma d-glucose concentration, 5.44 ± 1.94 μmol/mL) and again under hyperglycemia (average, 10.24 ± 1.44 μmol/mL). Time-radioactivity curves were obtained from healthy cortex (grey matter) and plasma and analyzed for the values of K1 and k2 by two graphical approaches and two fitting procedures. Both K1 and k2 significantly declined with increasing plasma glucose levels. A highly significant interindividual but not intraindividual variability for k2 was found at normoglycemia and hyperglycemia. The interindividual variability of K1, although borderline significant, was less than that of k2. Accordingly variable were the distribution volumes K1 /k2. These data suggest individualized glial cell function and may be relevant to pathogenesis of neuropsychiatric disease.
Commensal bacteria‐dependent select expression of CXCL2 contributes to periodontal tissue homeostasis

The oral and intestinal host tissues both carry a heavy microbial burden. Although commensal bacteria contribute to healthy intestinal tissue structure and function, their contribution to oral health is poorly understood. A crucial component of periodontal health is the recruitment of neutrophils to periodontal tissue. To elucidate this process, gingival tissues of specific‐pathogen‐free and germ‐free wild‐type mice and CXCR2KO and MyD88KO mice were examined for quantitative analysis of neutrophils and CXCR2 chemoattractants (CXCL1, CXCL2). We show that the recruitment ofneutrophils to the gingival tissue does not require commensal bacterial colonization but is entirely dependent on CXCR2 expression. Strikingly, however, commensal bacteria selectively upregulate the expression of CXCL2, but not CXCL1, in a MyD88‐dependent way that correlates with increased neutrophil recruitment as compared with germ‐free conditions. This is the first evidence that the selective use of chemokine receptor ligands contributes to neutrophil homing to healthy periodontal tissue.