Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of cancer stem cells from human glioblastomas: growth and differentiation capabilities and CD133/prominin‐1 expression

CD133 can be a marker of tumorigenic CSCs (cancer stem cells) in human GBM (glioblastoma multiforme), although tumorigenic CD133‐negative CSCs have been also isolated. Additional evidence indicates that CSCs from GBM exhibit different phenotypes, with increasing interest in the potential significance of the different CSCs with respect to diagnosis, prognosis and the development of novel targets for treatment. We have analysed the expression of CD133 in freshly isolated cells from 15 human GBM specimens. Only 4 of them contained cells positive for AC133 by FACS analysis, and all of them yielded distinct CSC lines, whereas only 6 CSC lines were obtained from the other 11 GBMs. Of these 10 CSCs lines, we further characterized 6 CSC lines. Three CSCs grew as fast‐growing neurospheres with higher clonogenic ability, whereas the remaining 3 grew as slow‐growing semi‐adherent spheres of lower clonogenicity. In addition, the former CSC lines displayed better differentiation capabilities than the latter ones. PCR and Western blot analysis showed that all 6 GBM CSC lines expressed CD133/prominin‐1, suggesting that cells negative by FACS analysis may actually represent cells expressing low levels of CD133 undetected by FACS. Nevertheless, all the 6 CSC lines were tumorigenic in nude mice. In conclusion, CSCs from human primary GBMs show different phenotypes and variable levels of CD133 expression, but these parameters did not directly correlate with the tumorigenic potential.
Identification and cloning of rat galectin-2: expression is predominantly in epithelial cells of the stomach.

A complementary DNA clone preferentially expressed in the gastrointestinal tract was obtained from a rat stomach library. The protein coded by the clone had a single carbohydrate recognition domain having conserved motifs for beta-galactoside binding and showed 67% amino acid identity with human galectin-2. The recombinant protein synthesized in Escherichia coli could bind to an asialofetuin column and was eluted with beta-galactopyranoside. From these observations, we named the protein rat galectin-2 coded by the cDNA. The rat galectin-2 was predominantly expressed in the epithelial cells of stomach. Thus this protein may form a mucin layer cross-linking with the beta-galactoside moiety of glycoproteins.
Dyslipidaemia in children on renal replacement therapy.

BACKGROUND Information on lipid abnormalities in end-stage renal disease (ESRD) mainly originates from adult patients and small paediatric studies. We describe the prevalence of dyslipidaemia, and potential determinants associated with lipid measures in a large cohort of paediatric ESRD patients. METHODS In the ESPN/ERA-EDTA registry, lipid measurements were available for 976 patients aged 2-17 years from 19 different countries from the year 2000 onwards. Dyslipidaemia was defined as triglycerides >100 mg/dL (2-9 years) or >130 mg/dL (9-17 years), high-density lipoprotein (HDL) cholesterol <40 mg/dL or non-HDL cholesterol >145 mg/dL. Missing data were supplemented using multiple imputation. RESULTS The prevalence of dyslipidaemia was 85.1% in peritoneal dialysis (PD) patients, 76.1% in haemodialysis (HD) patients and 55.5% among renal allograft recipients. Both low and high body mass index (BMI) were associated with a less favourable lipid profile. Younger age was associated with a worse lipid profile among PD patients. HDL levels significantly improved after transplantation, whereas no significant improvements were found for triglyceride and non-HDL levels. In transplant recipients, use of cyclosporin was associated with significantly higher non-HDL and HDL levels than tacrolimus usage (P < 0.01). In transplant patients with eGFR < 29 mL/min/1.73 m(2), the mean triglyceride level was 137 mg/dL (99% confidence interval (CI): 119-159) compared with 102 mg/dL among those with eGFR > 90 mL/min/1.73 m(2) (P < 0.0001). CONCLUSIONS Dyslipidaemia is common among paediatric ESRD patients in Europe. Young age and PD treatment are associated with worse lipid profiles. Although lipid levels generally improve after transplantation, dyslipidaemia may persist due to decreased graft function, high BMI or to the use of certain immunosuppressants.
Students' experiences in guided computer-based learning: A progressive evaluation

This study investigated students' experiences in two types of instructional strategies (i.e., fully-guided and partially guided) and their learning experience patterns from the beginning towards the end of the computer-based interactions. An empirical study was performed on 41 students from a public university in Malaysia using two types of computer-based systems for learning Basic Computer Networks. The students' learning experiences were evaluated at three different stages of the computer-based learning sessions using self-report technique. The results showed that the students' learning experiences were changing over the period of the computer-based lessons either in positive or negative ways. The directions of changes were mainly influenced by the students' Information Technology (IT) prior knowledge. The results of this study suggest that computer-based guided learning systems should consider students' background of knowledge in their design to achieve effective learning. Further, the combination of the fully-and partially-guided features may create an adaptive computer-based learning system.
Mechanisms of Political Radicalization: Pathways

ln this article we describe mechanisms of radicalization relevant to understanding the origins or terrorism. We must immediately acknowledge that the idea of mechunistn IS somewhat different in different domains of social science, sometimes with conflict­ ing definitions used within a single discipline. 1,2 Here we use mechanism in the gen­ eral sense traditionally employed in Psychology: "the means or mariner 111 \\ hieh something is accomplished. Thus, the mechanism of vision includes the physica. stimulus and the physiological and neural processes involved ,,~
Food intake and appetite following school-based high-intensity interval training in 9–11-year-old children

ABSTRACT Using a randomised cross-over design, free-living lunch intake and subjective appetite were examined in 10 children (9.8 ± 0.6 years) following high-intensity interval training (HIIT) versus a control sedentary (SED) period, within a school setting. The 22-min HIIT took place during a regular PE lesson and consisted of two rounds of 4 × 30 s sprints. Foods were offered at a regular school lunch immediately following HIIT and SED and were matched between conditions. All food was covertly weighed before and after the meal. Hunger, fullness and prospective consumption were reported immediately before and after HIIT/SED, using visual analogue scales. Heart rate was higher during HIIT than SED (159.3 ± 23.1 vs. 76.9 ± 2.2 bpm, P < 0.05). Lunch energy intake was not different (P = 0.52) following HIIT, compared to SED (2.06 ± 0.35 vs. 2.09 ± 0.29 MJ, respectively). There were no significant differences in macronutrient intake or subjective appetite (P > 0.05). Results suggest that HIIT can be implemented in a PE lesson immediately before lunch, without causing a compensatory increase in food consumption.
Population genetic structuring of methicillin-resistant Staphylococcus aureus clone EMRSA-15 within UK reflects patient referral patterns

Antibiotic resistance forms a serious threat to the health of hospitalised patients, rendering otherwise treatable bacterial infections potentially life-threatening. A thorough understanding of the mechanisms by which resistance spreads between patients in different hospitals is required in order to design effective control strategies. We measured the differences between bacterial populations of 52 hospitals in the United Kingdom and Ireland, using whole-genome sequences from 1085 MRSA clonal complex 22 isolates collected between 1998 and 2012. The genetic differences between bacterial populations were compared with the number of patients transferred between hospitals and their regional structure. The MRSA populations within single hospitals, regions and countries were genetically distinct from the rest of the bacterial population at each of these levels. Hospitals from the same patient referral regions showed more similar MRSA populations, as did hospitals sharing many patients. Furthermore, the bacterial populations from different time-periods within the same hospital were generally more similar to each other than contemporaneous bacterial populations from different hospitals. We conclude that, while a large part of the dispersal and expansion of MRSA takes place among patients seeking care in single hospitals, inter-hospital spread of resistant bacteria is by no means a rare occurrence. Hospitals are exposed to constant introductions of MRSA on a number of levels: (1) most MRSA is received from hospitals that directly transfer large numbers of patients, while (2) fewer introductions happen between regions or (3) across national borders, reflecting lower numbers of transferred patients. A joint coordinated control effort between hospitals, is therefore paramount for the national control of MRSA, antibiotic-resistant bacteria and other hospital-associated pathogens.
Open-Market Stock Repurchase Announcements and Revaluation of Prior Accounting Information

This study finds that, for a sample of 335 open-market repurchase announcements during 1978 to 1992, the market reaction to the announcement is significantly associated with the firm's sales growth and accounting profitability in prior periods. This result holds after controlling for two known correlates of the market response, the announced fraction to be repurchases and prior returns. This result is consistent in that the market reinterprets previously released accounting information when interpreting a subsequent repurchase announcement by the firm. Further, the association between the market response and prior accounting information is more pronounced for firms that are smaller in size or have fewer analysts following them. This suggests that the degree of reinterpretation of prior accounting information at the time of the repurchase announcement increases in the information asymmetry between managers and investors.
CASE-CONTROL STUDY OF LUNG CANCER RISK FROM RESIDENTIAL RADON EXPOSURE IN WORCESTER COUNTY, MASSACHUSETTS

A study of lung cancer risk from residential radon exposure and its radioactive progeny was performed with 200 cases (58% male, 42% female) and 397 controls matched on age and sex, all from the same health maintenance organization. Emphasis was placed on accurate and extensive year-long dosimetry with etch-track detectors in conjunction with careful questioning about historic patterns of in-home mobility. Conditional logistic regression was used to model the outcome of cancer on radon exposure, while controlling for years of residency, smoking, education, income, and years of job exposure to known or potential carcinogens. Smoking was accounted for by nine categories: never smokers, four categories of current smokers, and four categories of former smokers. Radon exposure was divided into six categories (model 1) with break points at 25, 50, 75, 150, and 250 Bq m−3, the lowest being the reference. Surprisingly, the adjusted odds ratios (AORs) were, in order, 1.00, 0.53, 0.31, 0.47, 0.22, and 2.50 with the third category significantly below 1.0 (p < 0.05), and the second, fourth, and fifth categories approaching statistical significance (p < 0.1). An alternate analysis (model 2) using natural cubic splines allowed calculating AORs as a continuous function of radon exposure. That analysis produces AORs that are substantially less than 1.0 with borderline statistical significance (0.048 ≤ p ≤ 0.05) between approximately 85 and 123 Bq m−3. College-educated subjects in comparison to high-school dropouts have a significant reduction in cancer risk after controlling for smoking, years of residency, and job exposures with AOR = 0.30 (95% CI: 0.13, 0.69), p = 0.005 (model 1).
Semimonthly oscillation observed in the start time of equatorial
Spread-F

Abstract. Using data from airglow an all sky imager and a coherent backscatter radar deployed at São João do Cariri (7.4° S, 36.5° W) and São Luís (2.6° S, 44.2° W), respectively, the start time of equatorial Spread-F were studied. Data from a period of over 10 years was investigated from 2000 to 2010. The semimonthly oscillations were clearly revealed in the start time of plasma bubbles from Oi6300 airglow images during three periods (September 2003, September–October 2005, November 2005 and January 2008). Since the airglow measurements are not continuous in time, more than one cycle of oscillation in the start time of plasma bubbles cannot be observed from these data. Thus, coherent backscatter radar data appeared as an alternative to investigate the start time of the ionospheric irregularities. Semimonthly oscillation were observed in the start time of plumes (November 2005) and bottom type Spread-F (November 2008) with at least one complete cycle. Technical/climate issues did not allowed to observe the semimonthly oscillations simultaneously by the two instruments, but from September to December 2005 there was a predominance of this spread-F start time oscillation over Brazil. The presence of this oscillation certainly contribute to the day-to-day variability of spread-F.
EPIDIDYMAL CYTOLOGY CHANGES IN HYPOTHYROID RATS

The purpose of this research was to investigate, using light, transmission, and scanning electron microscope, the effect of hypothyroidism on the ultrastructure of the rat epididymis. Thyroidectomy was obtained by ip injection of 270 w Ci of 131 I per rat. One month later, several portions of cauda epididymis were examined. Morphological differences were detected in the epididymis of the hypothyroid animals when compared to the control normal rats. The hypothyroid conditions were associated with important changes in the epididymis. The light observations showed cells with clearing of the chromatin and increased density and thickness of the chromatic rim, chromatinic net, and disappearance of the segment of the chromatin rim. In the scanning electron microscope broken, oblique, denuded epithelial cells with loss of stereocilia were observed, as well as flattening of the tubule. The hypothyroid condition under transmission electron microscope was associated with a decrease in the height of the cells, diminution of the internal lumen and number of mitoses, and decreased chromatin decondensation. Results obtained confirmed that hypothyroidism causes marked structural changes in the ductus epididymis and could adversely affect the maturation and motility of sperm.
A Skewed Student-T Value-at-Risk Approach for Long Memory Volatility Processes in Japanese Financial Markets

This paper investigates the relevance of skewed Student-t distributions in capturing long memory volatility properties in the daily return series of Japanese financial data (Nikkei 225 Index and JPY-USD exchange rate). For this purpose, we assess the performance of two long memory Value-at-Risk (VaR) models (FIGARCH and FIAPARCH VaR model) with three different distribution innovations: the normal, Student-t, and skewed Student-t distributions. From our results, we find that the skewed Student-t distribution model produces more accurate VaR estimations than normal and Student-t distribution models. Thus, accounting for skewness and excess kurtosis in the asset return distribution can provide suitable criteria for VaR model selection in the context of long memory volatility and enhance the performance of risk management in Japanese financial markets.
Prospective randomized trial of insulin glargine in acute management of diabetic ketoacidosis in the emergency department: a pilot study.

OBJECTIVES The objective was to determine the efficacy of coadministration of subcutaneous (SQ) insulin glargine in combination with intravenous (IV) insulin for treating diabetic ketoacidosis (DKA). METHODS This was a prospective, randomized, controlled trial comparing coadministration of insulin glargine and IV insulin (experimental) with IV insulin (standard care control). The setting was emergency departments (EDs) in two hospitals in Houston, Texas. Patients presenting with blood sugar ≥ 200 mg/dL, pH ≤ 7.3, bicarbonate (HCO3 ) ≤ 18 mg/dL, ketonemia or ketonuria, and anion gap ≥ 16 between November 2012 and April 2013 were enrolled. All patients received IV insulin. Additionally, the experimental group was given SQ insulin glargine within 2 hours of diagnosis. Upon closure of anion gap, patients in the control group were subsequently transitioned to long-acting insulin. In the study group, IV insulin was discontinued and long-acting SQ insulin was reinstituted 24 hours after initial introduction. The primary outcome of time to closure of anion gap (TCAG) was compared between groups using a general linear model (GLM), adjusting for initial anion gap, etiology, and presence of comorbidities. Similarly, the secondary outcome hospital length of stay (LOS) was adjusted for age, etiology, and hospital site in the GLM. Rate of hypoglycemia and intensive care unit (ICU) admission was compared using Fisher's exact test while ICU LOS was compared using Wilcoxon's two-sample test. RESULTS A total of 40 patients were enrolled in this pilot trial. The estimated mean TCAG was 10.2 hours (SE ± 6.8 hours) in the experimental group and 11.6 hours (SE ± 6.4 hours) in the control group (p = 0.63). The estimated mean hospital LOS was 3.9 days (SE ± 3.4 days) in the experimental group and 4.8 days (SE ± 3.6 days) in the control group (p = 0.66). Incidents of hypoglycemia, rates of ICU admission, and ICU LOS were similar between the groups. CONCLUSIONS Coadministration of glargine in combination with an insulin infusion in the acute management of DKA is feasible. Further study is needed to determine the true efficacy in terms of TCAG and hospital LOS.
The Transformation of Political Communication in China: From Propaganda to Hegemony

This book examines different dynamics such as marketisation, globalisation and new media technologies that have driven the transformation of China's media industry - one of the primary battlegrounds where ideological, social and economic struggles are fought - against the backdrop of the growing tensions between economic growth, globalisation, and political control in China.
The genetic ablation of SRC-3 protects against obesity and improves insulin sensitivity by reducing the acetylation of PGC-1α

Transcriptional control of metabolic circuits requires coordination between specific transcription factors and coregulators and is often deregulated in metabolic diseases. We characterized here the mechanisms through which the coactivator SRC-3 controls energy homeostasis. SRC-3 knock-out mice present a more favorable metabolic profile relative to their wild-type littermates. This metabolic improvement in SRC-3−/− mice is caused by an increase in mitochondrial function and in energy expenditure as a consequence of activation of PGC-1α. By controlling the expression of the only characterized PGC-1α acetyltransferase GCN5, SRC-3 induces PGC-1α acetylation and consequently inhibits its activity. Interestingly, SRC-3 expression is induced by caloric excess, resulting in the inhibition of PGC-1α activity and energy expenditure, whereas caloric restriction reduces SRC-3 levels leading to enhanced PGC-1α activity and energy expenditure. Collectively, these data suggest that SRC-3 is a critical link in a cofactor network that uses PGC-1α as an effector to control mitochondrial function and energy homeostasis.
The Baland-Robinson Theory of Child Labor Efficiency: A Proposed Extension

This paper is an extension of the Baland and Robinson theory of child labor efficiency which studies the problem of child labor using a two period model with a general equilibrium approach. To the original theory, we seek to incorporate a non-negative cost of education and an indicator for school quality. Our findings suggest that an increase in costs of education invariably raises the level of child labor. Furthermore, when school quality is poor, improvement in it can decrease the equilibrium level of child labor. In the welfare analysis we find that the conditions for education subsidies to be welfare-increasing suffice for a ban on child labor to be Pareto improving.
Enhanced early sensory outcome after nerve repair as a result of immediate post-operative re-learning: a randomized controlled trial

We assessed the use of guided plasticity training to improve the outcome in the first 6 months after nerve repair. In a multicentre randomized controlled trial, 37 adults with median or ulnar nerve repair at the distal forearm were randomized to intervention, starting the first week after surgery with sensory and motor re-learning using mirror visual feedback and observation of touch, or to a control group with re-learning starting when reinnervation could be detected. The primary outcome at 3 and 6 months post-operatively was discriminative touch (shape texture identification test, part of the Rosen score). At 6 months, discriminative touch was significantly better in the early intervention group. Improvement of discriminative touch between 3 and 6 months was also significantly greater in that group. There were no significant differences in motor function, pain or in the total score. We conclude that early re-learning using guided plasticity may have a potential to improve the outcomes after nerve repair. Level of evidence: II
Error Analysis of Limb and Orofacial Praxis in Children with Developmental Motor Deficits

Two models have been suggested to depict the relationship between disorders of limb and orofacial praxis. The first views apraxia as a unitary disorder in which the underlying mechanisms for each type are similar, while the second model suggests that there are two separate praxis systems: one for planning and controlling limb gestures and a second one for planning and controlling orofacial movements. The purpose of this study was to investigate whether a common mechanism may underlie deficits in limb and orofacial praxis in children. This was done by analyzing the types of praxis errors demonstrated by children with developmental motor deficits and normal controls when performing limb and orofacial gestures. Results indicated that there was consistency across modalities (i.e., limb, orofacial) in the types of praxis errors made by children with motor deficits, providing support for the idea that a common mechanism may underlie disruptions to limb and orofacial praxis in children. This study also examined developmental trends in gestural representation and in types of praxis errors. The findings revealed a striking developmental maturation in gestural ability between the ages of 6 and 11 years for all children. However, over this age range, children with developmental motor deficits were impaired relative to normal controls.
The Geographic Mobility of Professional and Technical Manpower

Professional, technical, and kindred workers are the fastest growing segment of the labor force; evidence from the 1960 Census reveals that they are close to twice as migratory as any other occupational stratum. An attempt is made to discover the major determinants of professional mobility, using the 1960 Census of Population one-in-a-thousand sample. In a multiple regression analysis age accounts for most of the explained variance in mobility, followed by income, education, regional location, sex, family size and marital status. Factor analysis suggests that migration is linked primarily to occupational career mobility and family life cycle. Occupational determinants are more closely scrutinized in a breakdown of detailed professional occupations by mobility. The analysis suggests several characteristics of work organizations and careers that influence geographic mobility. Other sources of mobility are discussed, and the relevance of the study for research on labor mobility is noted.