Miracle-dz/UniFAR-FTUs huggingface dataset:
Litter Survival Differences between Divergently Selected Lines for Environmental Sensitivity in Rabbits

Simple Summary Two rabbit lines are divergently selected for increasing or decreasing the variability of litter size at birth. Decreasing the litter size variability produces more resilient females with less sensitivity to diseases, being an indirect selection way to improve environmental sensitivity. The kits’ survival rate at weaning was higher in the homogeneous line. Moreover, this line led to a greater uniformity of the kits’ weight at weaning, although the weight variability at birth was higher, which could be due to a higher lactation capacity of the homogeneous line. Abstract A divergent selection experiment on environmental sensitivity was performed in rabbits. The aim was to estimate the correlated response in kit weight and survival, litter weight, and weight distance from birth to weaning. The weight distance was calculated as the absolute value of the differences between the individual value and the mean value of its litter. The relationship between the probability of survival at 4 d of age, and the weight at birth, was studied. Environmental sensitivity was measured as litter size variability. A total of 2484 kits from 127 does from the low line, and 1916 kits of 114 does from the high line of the 12th generation were weighed. Both of the lines showed similar individual and litter weights at birth and weaning, and a similar survival rate at birth, and at 4 d of age. The survival rate at weaning was higher in the low line (0.67 and 0.62; P = 0.93). The weight distance was higher at birth, but lower at weaning in the low line (47.8 g and 54.1 g; P = 0.98). When the weight at birth was high, the kits had a higher survival rate. In conclusion, selection for environmental sensitivity showed a correlated response in the kits’ survival, and in the homogeneity of litter weight at weaning.
Three-dimensional kinematics in healthy older adult males during golf swings

ABSTRACT The biomechanics of the golf swing have received considerable attention in previous research. However, existing studies have focused on young athletes, while the kinematics of older golfers remain poorly documented. This study presents kinematic data for healthy senior golfers during swings performed with a driver and six-iron. Seventeen male golfers (62.2 ± 8.8 years) volunteered for participation and a 10-camera Vicon system (Oxford, UK) recorded kinematic data (500 Hz). A launch monitor (TrackMan, Vedbæk, Denmark) recorded club head speed and initial ball speed. Joint angles and peak velocities of the trunk and lower body were extracted at the top of the backswing, ball contact, and end of the swing. Intraclass correlations and standard error of measurement determined reliability, and pairwise statistics determined between-club differences. Swings with the driver had 7.3° less trunk extension and 4.3° less X-factor at backswing, and 10.5° less trunk flexion and 3.2° less X-factor at ball impact. Older adults portray several differences in lower body kinematics between a six-iron and driver but maintain good to excellent reliability (0.728–0.997) during the swings. Comparisons with previous research also showed senior athletes produce slower club head and ball speeds than younger golfers, and that kinematic differences exist between the populations.
Adipose Mesenchymal Extracellular Vesicles as Alpha-1-Antitrypsin Physiological Delivery Systems for Lung Regeneration

Accumulating evidence shows that Mesenchymal Stem/Stromal Cells (MSCs) exert their therapeutic effects by the release of secretome, made of both soluble proteins and nano/microstructured extracellular vesicles (EVs). In this work, for the first time, we proved by a proteomic investigation that adipose-derived (AD)-MSC-secretome contains alpha-1-antitrypsin (AAT), the main elastase inhibitor in the lung, 72 other proteins involved in protease/antiprotease balance, and 46 proteins involved in the response to bacteria. By secretome fractionation, we proved that AAT is present both in the soluble fraction of secretome and aggregated and/or adsorbed on the surface of EVs, that can act as natural carriers promoting AAT in vivo stability and activity. To modulate secretome composition, AD-MSCs were cultured in different stimulating conditions, such as serum starvation or chemicals (IL-1β and/or dexamethasone) and the expression of the gene encoding for AAT was increased. By testing in vitro the anti-elastase activity of MSC-secretome, a dose-dependent effect was observed; chemical stimulation of AD-MSCs did not increase their secretome anti-elastase activity. Finally, MSC-secretome showed anti-bacterial activity on Gram-negative bacteria, especially for Klebsiella pneumoniae. These preliminary results, in addition to the already demonstrated immunomodulation, pave the way for the use of MSC-secretome in the treatment of AAT-deficiency lung diseases.
The remote allosteric control of Orai channel gating

Calcium signals drive an endless array of cellular responses including secretion, contraction, transcription, cell division, and growth. The ubiquitously expressed Orai family of plasma membrane (PM) ion channels mediate Ca2+ entry signals triggered by the Ca2+ sensor Stromal Interaction Molecule (STIM) proteins of the endoplasmic reticulum (ER). The 2 proteins interact within curiously obscure ER-PM junctions, driving an allosteric gating mechanism for the Orai channel. Although key to Ca2+ signal generation, molecular understanding of this activation process remain obscure. Crystallographic structural analyses reveal much about the exquisite hexameric core structure of Orai channels. But how STIM proteins bind to the channel periphery and remotely control opening of the central pore, has eluded such analysis. Recent studies apply both crystallography and single-particle cryogenic electron microscopy (cryo-EM) analyses to probe the structure of Orai mutants that mimic activation by STIM. The results provide new understanding on the open state of the channel and how STIM proteins may exert remote allosteric control of channel gating.
CD4+ count‐dependent concentration–effect relationship of rituximab in rheumatoid arthritis

Rituximab is approved in rheumatoid arthritis (RA). A substantial decrease in CD4+ count was observed in responders after a single cycle of treatment. This study aimed to describe and quantifying the influence of CD4+ count depletion on the concentration–response relationship of rituximab in RA patients.
Analysis of probe fitting stimulus properties on transient evoked otoacoustic emissions

Abstract Objective: The objectives of the current study are first, to evaluate the subjective evaluation of probe fitting stimulus properties, and second, to analyse the effects of different probe fittings on transient evoked otoacoustic emission (TEOAE) response and noise amplitudes in subjects. Design: The Q methodology was used to sort 48 probe fittings differing in stimulus properties in seven categories from totally unacceptable to certainly acceptable. Further, TEOAE response and noise amplitudes were measured in one baseline condition with optimal probe fitting and eight experimental conditions with less than optimal probe fittings. Study sample: The probe fittings were ranked by 18 participants, while the repeated measures design was performed in 34 ear. Results: First, it was found that only 19.61% of all distributions of the probe fittings by the participants had a mutual correlation of at least 70.00%. Almost 60% of the variance of distributions was explained by 83.33% of the participants, although most probe fittings significantly differed from other fittings based on spectral broadness. Second, significant differences in TEOAE response and especially noise amplitudes between conditions were found. Further, TEOAE response and noise amplitudes between the baseline and experimental conditions were significantly different depending on ringing and spectral flatness of the stimulus. Conclusion: A substantial amount of subjectivity during TEOAE measurements is involved with regard to the evaluation of probe fitting stimulus properties. TEOAE response but especially noise amplitudes are influenced by varying stimulus parameters which stresses the importance of inspecting these parameters prior to or during EOAE measurements. Although more research is needed, some guidelines regarding these parameters are given which could improve the accuracy of TEOAEs in practice.
MR‐based age‐related effects on the striatum, globus pallidus, and thalamus in healthy individuals across the adult lifespan

While numerous studies have used magnetic resonance imaging (MRI) to elucidate normative age‐related trajectories in subcortical structures across the human lifespan, there exists substantial heterogeneity among different studies. Here, we investigated the normative relationships between age and morphology (i.e., volume and shape), and microstructure (using the T1‐weighted/T2‐weighted [T1w/T2w] signal ratio as a putative index of myelin and microstructure) of the striatum, globus pallidus, and thalamus across the adult lifespan using a dataset carefully quality controlled, yielding a final sample of 178 for the morphological analyses, and 162 for the T1w/T2w analyses from an initial dataset of 253 healthy subjects, aged 18–83. In accordance with previous cross‐sectional studies of adults, we observed age‐related volume decrease that followed a quadratic relationship between age and bilateral striatal and thalamic volumes, and a linear relationship in the globus pallidus. Our shape indices consistently demonstrated age‐related posterior and medial areal contraction bilaterally across all three structures. Beyond morphology, we observed a quadratic inverted U‐shaped relationship between T1w/T2w signal ratio and age, with a peak value occurring in middle age (at around 50 years old). After permutation testing, the Akaike information criterion determined age relationships remained significant for the bilateral globus pallidus and thalamus, for both the volumetric and T1w/T2w analyses. Our findings serve to strengthen and expand upon previous volumetric analyses by providing a normative baseline of morphology and microstructure of these structures to which future studies investigating patients with various disorders can be compared.
Efficacy of an organophosphorus hydrolase enzyme (OpdA) in human serum and minipig models of organophosphorus insecticide poisoning

Abstract Objectives: Current therapeutic options for organophosphorus (OP) insecticide self-poisoning including atropine and oximes are inadequate and case fatality may exceed 20%. An OP hydrolase enzyme, OpdA, has been used for environmental cleansing of OP insecticides and prevented death in rat and non-human primate models of OP insecticide poisoning if given very quickly after exposure. We here tested OpdA’s ability to break down OP insecticides in human serum and in clinically relevant minipig models of OP insecticide poisoning. Methods: Human serum was spiked with seven diverse WHO Class II OP insecticides (chlorpyrifos, quinalphos, diazinon, dimethoate, fenthion, phenthoate, and profenofos) and the effect of OpdA on degradation measured. The pharmacodynamic and clinical effects of OpdA treatment were studied in Gottingen minipigs orally poisoned with agricultural formulations of dimethoate EC40 or methyl parathion EC60; pharmacodynamic effects were also assessed in profenofos EC50-poisoned pigs. Results: OpdA effectively hydrolysed OP insecticides in human serum, with rates varying from 856 (SD 44) down to 0.107 (SD 0.01) moles of substrate hydrolysed/mole of enzyme/sec (kcat) for quinalphos and phenthoate, respectively, although at rates 2–3 log orders less than found in vitro in buffered solution. It showed clinical benefit in minipig models, reducing the dose of noradrenaline required to sustain an adequate mean arterial pressure after dimethoate (mean 0.149 [SD 0.10] μg/kg/h vs. 1.07 [SD 0.77] μg/kg/h, p < .0001) and methyl parathion (mean 0.077 [SD 0.08] μg/kg/h vs. 0.707 [SD 0.49] μg/kg/h, p < .0001) poisoning. OpdA reduced blood OP insecticide concentration and acetylcholinesterase inhibition after poisoning by dimethoate, methyl parathion, and profenofos insecticides. Conclusions: In vitro incubation of OpdA in human serum showed hydrolysis of diverse OP insecticides, although at lower rates than found in buffer solutions. This activity results in clinical and pharmacodynamic efficacy in vivo against several OP insecticides. These results support the testing of OpdA in further animal models before considering human trials to determine whether it may become an urgently required novel therapeutic agent for OP insecticide self-poisoning.
Identification of Key Residues Required for RNA Silencing Suppressor Activity of p23 Protein from a Mild Strain of Citrus Tristeza Virus

The severe strain of citrus tristeza virus (CTV) causes quick decline of citrus trees. However, the CTV mild strain causes no symptoms and commonly presents in citrus trees. Viral suppressor of RNA silencing (VSR) plays an important role in the successful invasion of viruses into plants. For CTV, VSR has mostly been studied in severe strains. In this study, the N4 mild strain in China was sequenced and found to have high sequence identity with the T30 strain. Furthermore, we verified the functions of three VSRs in the N4 strain, and p23 was found to be the most effective in terms of local silencing suppressor activity among the three CTV VSRs and localized to both nucleus and plasmodesmata, which is similar to CTV T36 strain. Several conserved amino acids were identified in p23. Mutation of E95A/V96A and M99A/L100AA impaired p23 protein stability. Consequently, these two mutants lost most of its suppressor activity and their protein levels could not be rescued by co-expressing p19. Q93A and R143A/E144A abolished p23 suppressor activity only and their protein levels increased to wild type level when co-expressed with p19. This work may facilitate a better understanding of the pathogenic mechanism of CTV mild strains.
Dissertations 2011

The annual list of doctoral dissertations completed and in-progress provides a reference and record for graduate students, their advisors, and all scholars of Spanish and Portuguese language, literatures, linguistics, and cultures. We have queried all departments that offer the PhD degree in these areas. These departments provide the students, titles, and categories listed. We express sincere appreciation to the departmental representatives who responded to the request for this information. We also ask forgiveness in advance for potential errors and omissions in receiving, interpreting, and compiling this large amount of complicated data.
EVENTS AS PERCEIVED PANACEA FOR SUSTAINABLE TOURISM DEVELOPMENT IN DURBAN , SOUTH AFRICA

Sustainability has become one of the key elements of development for most tourist destinations. Some cities have adopted a sustainable development strategy as a long-term solution in improving socio-economic, cultural, and environmental practices. Traditionally, destinations have used events to achieve community enrichment, protect the natural environment, and create or maintain national pride. The aim of this research was to analyse the impact of events on sustainable tourism development in Durban. The study analysed the perceptions of key stakeholders regarding the contribution of events to socio-economic growth of the city of Durban. In carrying out the research, a mixed method approach was used. A total of 400 questionnaires were administered and 25 in-depth interviews were conducted. The results show that there are economic spin offs resulting from visitor spending during major events in the City. Furthermore, the study shows that although events can be used as a tool for sustainable tourism development, the challenge is the unbalanced racial structure within the industry, poor state of the economy, and limited access to business start-up capital for SMMEs. The study concludes that events play a vital role in socio-economic growth and development of the tourism sector in Durban and recommends multi-stakeholder collaboration and partnerships to ensure sustained tourism growth.
An Analysis of Culture-Related Content in English Textbooks for Iranian Students Entitled ‘ Prospect ’ and ‘ Vision ’ Series

The treatment of culture in ELT textbooks has a considerable impact on students’ understanding of cultures. The present study is intended to examine the cultural representation in ELT text books used in Iran. Throughout the study, this issue was put under investigation using Ramirez and Halls’ modified version model. The study revealed that the textbook developers had only home culture in their minds which is inadequate in fostering intercultural communicative competence and with respect to their culture treatments, there are no considerable differences among the textbooks: That is, a similar condition was observed. Throughout the textbooks, ‘pictures’ with source culture references and sentences in ‘conversation’ sections also with home culture references covered the largest portions and only rare of the target culture appeared.
Implementation of a Two-Phase Material Model With Strength : Application to Porous Materials Executive Summary

A material model accounting for strength developed earlier for two-phase materials is implemented in the CTH hydrocode. The strain response to load in the model is decoupled into shear and volumetric contributions in order to satisfy the model implementation requirements for CTH. Multi-phase description is realised via constitutive equations complementing the conservation laws for a material represented as a mixture of several phases. Such a formulation agrees well with the CTH code structure and is suitable for conventional user implementation. The implementation has been applied to a generic material representing sand at various porosities. The constitutive equations and equations of state have been fitted in order to describe literature data. Numerical illustrations in the report demonstrate agreement of the calculation results with the anomalous behaviour observed in the literature for a highly porous sand at shock compression and a good description of the experiments available in the literature on the explosion of a sand-buried charge.
' s personal copy Reciprocal relationships between job resources , personal resources , and work engagement

This study examined longitudinal relationships between job resources, personal resources, and work engagement. On the basis of Conservation of Resources theory, we hypothesized that job resources, personal resources, and work engagement are reciprocal over time. The study was conducted among 163 employees, who were followed-up over a period of 18 months on average. Results of structural equation modeling analyses supported our hypotheses. Specifically, we found that T1 job and personal resources related positively to T2 work engagement. Additionally, T1 work engagement related positively to T2 job and personal resources. The model that fit best was the reciprocal model, which showed that not only resources and work engagement but also job and personal resources were mutually related. These findings support the assumption of Conservation of Resources theory that various types of resources and well-being evolve into a cycle that determines employees’ successful adaptation to their work environments. 2008 Elsevier Inc. All rights reserved.
Vegetative and Reproductive Variability of Dictyota crenulata (Phaeophyta: Dictyotales) along the Central and Southwestern Gulf of California, México

Dictyota crenulata J. Agardh is widely distributed throughout the Gulf of California. Comparative analyses of morphology, anatomy, and reproductive features of this species were conducted along the central western and southwestern regions of the Gulf of California. Thalli showed geographical variations in length and apical width. No differences were observed in anatomy of vegetative thalli or relative abundance of reproductive structures. Dictyota crenulata had unilayered or multilayered medullas in the basal region and in proliferations. Most thalli presented unilayered medullas in the middle section. Our observations indicate that number of medullary layers is indeed a phenotypically plastic character, in agreement with previously published results. Variations in thallus morphology such as proliferations and length are likely the result of environmental differences, also reflected in the reproduction of D. crenulata. The southwestern region had the highest percentage of all life cycle stages (female gametophytes and sporophytes, both 22%, and vegetative thalli, 14%). Our results demonstrate morphological variability in Dictyota crenulata across its distribution in the Gulf of California.
A New Dimeric Sesquiterpenoid from Chloranthus japonicus Sieb

Phytochemical investigation into the whole plants of Chloranthus japonicus Sieb. led to isolation and identification of a new dimeric lindenane sesquiterpenoid, named chlojapolactone B (1), and two new phenolic derivatives (2 and 3). Their chemical structures were elucidated on the basis of HRESIMS and NMR spectroscopic data, and the absolute configuration of 1 was determined using the electronic circular dichroism (ECD) analysis.
Cytochrome P 450 1 B 1 contributes to increased blood pressure and cardiovascular and renal dysfunction in spontaneously hypertensive rats

Purpose—We investigated the contribution of cytochrome P450 (CYP) 1B1 to hypertension and its pathogenesis by examining the effect of its selective inhibitor, 2,4,3′,5′-tetramethoxystilbene (TMS), in spontaneously hypertensive rats (SHR). Methods—Blood pressure (BP) was measured bi-weekly. Starting at 8 weeks, TMS (600 μg/kg, i.p.) or its vehicle was injected daily. At 14 weeks, samples were collected for measurement. Results—TMS reversed increased BP in SHR (207±7 vs. 129±2 mmHg) without altering BP in Wistar-Kyoto rats. Increased CYP1B1 activity in SHR was inhibited by TMS (RLU: aorta, 5.4±0.7 vs. 3.7±0.7; heart, 6.0±0.8 vs. 3.4±0.4; kidney, 411±45 vs. 246±10). Increased vascular reactivity, cardiovascular hypertrophy, endothelial and renal dysfunction, cardiac and renal fibrosis in SHR were minimized by TMS. Increased production of reactive oxygen species and NADPH oxidase activity in SHR, were diminished by TMS. In SHR, TMS reduced increased plasma levels of nitrite/nitrate (46.4±5.0 vs. 28.1±4.1 μM), hydrogen-peroxide (36.0±3.7 vs. 14.1±3.8 μM), and thiobarbituric acid reactive substances (6.9±1.0 vs. 3.4±1.5 μM). Increased plasma levels of pro-inflammatory cytokines and catecholamines, and cardiac activity of extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, c-Srctyrosine kinase, and protein kinase B in SHR were also inhibited by TMS. Conclusions—These data suggests that increased oxidative stress generated by CYP1B1 contributes to hypertension, increased cytokine production and sympathetic activity, and associated pathophysiological changes in SHR. CYP1B1 could be a novel target for developing drugs to treat hypertension and its pathogenesis. © Springer Science+Business Media New York 2014