Miracle-dz/UniFAR-FTUs huggingface dataset:
Regenerating β cells of the pancreas – potential developments in diabetes treatment

ABSTRACT Introduction: The etiology of diabetes is mainly attributed to insulin deficiency due to the lack of β cells (type 1), or to insulin resistance that eventually results in β cell dysfunction (type 2). Therefore, an ultimate cure for diabetes requires the ability to replace the lost insulin-secreting β cells. Strategies for regenerating β cells are under extensive investigation. Areas covered: Herein, the authors first summarize the mechanisms underlying embryonic β cell development and spontaneous adult β cell regeneration, which forms the basis for developing β cell regeneration strategies. Then the rationale and progress of each β cell regeneration strategy is reviewed. Current β cell regeneration strategies can be classified into two main categories: in vitro β cell regeneration using pluripotent stem cells and in vivo reprogramming of non-β cells into β cells. Each has its own advantages and disadvantages. Expert opinion: Regenerating β cells has shown its potential as a cure for the treatment of insulin-deficient diabetes. Much progress has been made, and β cell regeneration therapy is getting closer to a clinical reality. Nevertheless, more hurdles need to be overcome before any of the strategies suggested can be fully translated from bench to bedside.
Effectiveness of an intervention promoting the female condom to patients at sexually transmitted disease clinics.

OBJECTIVES This study evaluated a behavioral intervention designed to promote female condoms and reduce unprotected sex among women at high risk for acquiring sexually transmitted diseases (STDs). METHODS The effect of the intervention on barrier use was evaluated with a pretest-posttest design with 1159 female STD clinic patients. RESULTS Among participants with follow-up data, 79% used the female condom at least once and often multiple times. More than one third of those who completed the study used female condoms throughout follow-up. Use of barrier protection increased significantly after the intervention, and high use was maintained during a 6-month follow-up. To account for attrition, the use of protection by all subjects was projected under 3 conservative assumptions. The initial visit and termination visit projections suggest that use increased sharply after the intervention and declined during follow-up but remained elevated compared with the baseline. CONCLUSIONS Many clients of public STD clinics will try, and some will continue, to use female condoms when they are promoted positively and when women are trained to use them correctly and to promote them to their partners. A behavioral intervention that promotes both female and male condoms can increase barrier use.
Reliability and validity of trunk assessment for people with multiple sclerosis.

BACKGROUND AND PURPOSE Standardized scales are a prerequisite for rehabilitation and research. This study was designed to determine the reliability and validity of scores on items of the trunk assessment of the Melsbroek Disability Scoring Test (MDST) and Trunk Impairment Scale (TIS) in people with multiple sclerosis (MS). SUBJECTS Thirty people with MS participated in the study. METHODS Interrater and test-retest reliability and construct validity were assessed. RESULTS Kappa and weighted kappa values for the items of the trunk assessment of the MDST ranged from . 74 to . 95, and the kappa and weighted kappa values for the TIS items ranged from . 46 to 1.00. Intraclass correlation coefficients for interrater and test-retest agreement were . 93 and . 92, respectively, for the trunk assessment of the MDST and . 97 and . 95, respectively, for the TIS. Bland-Altman analysis showed consistency of scores without observer bias. Construct validity was established. DISCUSSION AND CONCLUSION The MDST and TIS provide reliable assessments of the trunk and are valid scales for measuring trunk performance in people with MS.
AGENTS, PRINCIPALS, OR SOMETHING IN BETWEEN? BUREAUCRATS AND POLICY CONTROL IN THAILAND

Abstract In the aftermath of the 2006 and 2014 Thai coups, observers declared the resurrection of the bureaucratic polity. Bureaucrats, though, remained influential even during the period of 1992–2006, when elected politicians were thought to command the Thai state. Bureaucratic involvement in politics poses a challenge for dominant political science theories of politician–bureaucrat relationships, which draw heavily from principal–agent frameworks. I apply agency theory to Thailand, testing three different hypotheses derived from the theory. Examining legislative productivity and control over bureaucratic career trajectories, I find that elected politicians increasingly acted as principals of the Thai state from 1992 through 2006, and to a lesser degree from 2008 to 2013. Thai bureaucrats, though, have frequently engaged in the political sphere, blunting political oversight and expanding their independence vis-à-vis politicians. This suggests that the principal–agent model overlooks the range of resources that bureaucracies can bring to bear in developing countries, granting them greater autonomy than anticipated. As such, theories of the politician–bureaucrat relationship in developing states need to better account for the mechanisms through which bureaucrats exercise policy discretion and political influence.
Forecasting of Jump Arrivals in Stock Prices: New Attention-based Network Architecture using Limit Order Book Data

The existing literature provides evidence that limit order book data can be used to predict short-term price movements in stock markets. This paper proposes a new neural network architecture for predicting return jump arrivals in equity markets with high-frequency limit order book data. This new architecture, based on Convolutional Long Short-Term Memory with Attention, is introduced to apply time series representation learning with memory and to focus the prediction attention on the most important features to improve performance. The data set consists of order book data on five liquid U.S. stocks. The use of the attention mechanism makes it possible to analyze the importance of the inclusion limit order book data and other input variables. By using this mechanism, we provide evidence that the use of limit order book data was found to improve the performance of the proposed model in jump prediction, either clearly or marginally, depending on the underlying stock. This suggests that path-dependence in limit order book markets is a stock specific feature. Moreover, we find that the proposed approach with an attention mechanism outperforms the multi-layer perceptron network as well as the convolutional neural network and Long Short-Term memory model.
Carbon dioxide laser‐assisted nerve repair: Effect of solder and suture material on nerve regeneration in rat sciatic nerve

In order to further improve and explore the role of lasers for nerve reconstruction, this study was designed to investigate regeneration of sharply transected peripheral nerves repaired with a CO2 milliwatt laser in combination with three different suture materials and a bovine albumin protein solder as an adjunct to the welding process. Unilateral sciatic nerve repair was performed in 44 rats. In the laser group, nerves were gently apposed, and two stay sutures (10‐0 nylon, 10‐0 polyglycolic acid, or 25‐μm stainless steel) were placed epi/perineurially. Thereafter, the repair site was fused at 100 mW with pulses of 1.0 s. In the subgroup of laser‐assisted nerve repair (LANR), albumen was used as a soldering agent to further reinforce the repair site. The control group consisted of nerves repaired by conventional microsurgical suture repair (CMSR), using 4–6 10‐0 nylon sutures. Evaluation was performed at 1 and 6 weeks after surgery, and included qualitative and semiquantitative light microscopy. LANR performed with a protein solder results in a good early peripheral nerve regeneration, with an optimal alignment of nerve fibers and minimal connective tissue proliferation at the repair site. All three suture materials produced a foreign body reaction; the least severe was with polyglycolic acid sutures. CMSR resulted in more pronounced foreign‐body granulomas at the repair site, with more connective‐tissue proliferation and axonal misalignment. Furthermore, axonal regeneration in the distal nerve segment was better in the laser groups. Based on these results, CO2 laser‐assisted nerve repair with soldering in combination with absorbable sutures has the potential of allowing healing to occur with the least foreign‐body reaction at the repair site. Further experiments using this combination are in progress. © 2003 Wiley‐Liss, Inc. MICROSURGERY 23:109–116 2003
Graves' ophthalmopathy: a preventable disease?

Most patients with Graves' disease have some degree of ocular involvement, but only 3-5% of them develop severe ophthalmopathy (1). The reasons why only such a minority of patients with Graves' disease have severe expression of the ophthalmopathy remain to be elucidated. One possible explanation is that non-severe ophthalmopathy and severe ophthalmopathy are two different disorders with different genetic backgrounds; alternatively, they might be part of a spectrum of different conditions ranging from absent ocular involvement to most severe ophthalmopathy. In this case, external variables (i.e. environmental factors) must contribute to the nature of the expression of the disease. How important are they? How far can our intervention on environmental factors go towards reducing the risk of progression of the ophthalmopathy? In other words, to which extent, if any, is Graves' ophthalmopathy preventable? The aim of this mini-review is to address the above issues.
Diagnostic Performance of an Advanced Modeled Iterative Reconstruction Algorithm for Low-Contrast Detectability with a Third-Generation Dual-Source Multidetector CT Scanner: Potential for Radiation Dose Reduction in a Multireader Study.

PURPOSE To assess the effect of radiation dose reduction on low-contrast detectability by using an advanced modeled iterative reconstruction (ADMIRE; Siemens Healthcare, Forchheim, Germany) algorithm in a contrast-detail phantom with a third-generation dual-source multidetector computed tomography (CT) scanner. MATERIALS AND METHODS A proprietary phantom with a range of low-contrast cylindrical objects, representing five contrast levels (range, 5-20 HU) and three sizes (range, 2-6 mm) was fabricated with a three-dimensional printer and imaged with a third-generation dual-source CT scanner at various radiation dose index levels (range, 0.74-5.8 mGy). Image data sets were reconstructed by using different section thicknesses (range, 0.6-5.0 mm) and reconstruction algorithms (filtered back projection [FBP] and ADMIRE with a strength range of three to five). Eleven independent readers blinded to technique and reconstruction method assessed all data sets in two reading sessions by measuring detection accuracy with a two-alternative forced choice approach (first session) and by scoring the total number of visible object groups (second session). Dose reduction potentials based on both reading sessions were estimated. Results between FBP and ADMIRE were compared by using both paired t tests and analysis of variance tests at the 95% significance level. RESULTS During the first session, detection accuracy increased with increasing contrast, size, and dose index (diagnostic accuracy range, 50%-87%; interobserver variability, ±7%). When compared with FBP, ADMIRE improved detection accuracy by 5.2% on average across the investigated variables (P < .001). During the second session, a significantly increased number of visible objects was noted with increasing radiation dose index, section thickness, and ADMIRE strength over FBP (up to 80% more visible objects, P < .001). Radiation dose reduction potential ranged from 56% to 60% and from 4% to 80% during the two sessions, respectively. CONCLUSION Low-contrast detectability performance increased with increasing object size, object contrast, dose index, section thickness, and ADMIRE strength. Compared with FBP, ADMIRE allows a substantial radiation dose reduction while preserving low-contrast detectability. Online supplemental material is available for this article.
Robust portfolio decisions for financial institutions

The present paper aims to study a robust-entropic optimal control problem arising in the management of financial institutions. More precisely, we consider an economic agent who manages the portfolio of a financial firm. The manager has the possibility to invest part of the firm's wealth in a classical Black-Scholes type financial market, and also, as the firm is exposed to a stochastic cash flow of liabilities, to proportionally transfer part of its liabilities to a third party as a means of reducing risk. However, model uncertainty aspects are introduced as the manager does not fully trust the model she faces, hence she decides to make her decision robust. By employing robust control and dynamic programming techniques, we provide closed form solutions for the cases of the (ⅰ) logarithmic; (ⅱ) exponential and (ⅲ) power utility functions. Moreover, we provide a detailed study of the limiting behavior, of the associated stochastic differential game at hand, which, in a special case, leads to break down of the solution of the resulting Hamilton-Jacobi-Bellman-Isaacs equation. Finally, we present a detailed numerical study that elucidates the effect of robustness on the optimal decisions of both players.
Impact of syringe size on the performance of infusion pumps at low flow rates

Objective: To evaluate the impact of syringe size on start-up delay and the time to reach 50% and 90% of target flow rates, using two commercially available syringe infusion pumps at infusion rates of ≤1 mL/hr. Design: Two syringes (Terumo) of different size (10-mL and 50-mL), using two syringe infusion pumps (Pump A, Terumo Terufusion Infusion Pump TE-331; and Pump B, Braun Perfusor Compact S) were studied. Effective fluid delivery was measured at 0.4 mL/hr, 0.8 mL/hr, and 1.0 mL/hr for the initial 60 mins, using the gravimetric method. Instant flow was calculated as volume difference for every 1-min interval per minute. Start-up delay was defined as time in minutes of 0 flow from the start of infusion. Syringe placement, bubble removal, infusion line priming, and positioning were standardized for all measurements, using new syringes and infusion lines. Each experiment was repeated six times. Statistical analysis was performed, using a nonparametric test (Mann-Whitney U test). Setting: None. Patients: None. Interventions: None. Results: Using the 50-mL syringe, the start-up delay was consistently higher and the time to reach 50% and 90% of target flow were significantly longer, independent of which syringe infusion pump was used. At every flow rate studied, the pumps did not reach the target flow rate before 60 mins with the 50-mL syringe. With the 10-mL syringe, target flow rate was achieved before 20 mins for both pumps. Conclusions: Our findings demonstrate a clinically relevant impact of syringe size on syringe infusion pump performance at low flow rates. The time to reach 50% and 90% of target flow are significantly longer, using the 50-mL syringe compared with the 10-mL syringe, and the time to reach 50% of target flow is independent of the longer start-up delay. Based on our findings, we speculate that smaller syringe sizes and higher infusion rates are preferable for continuous drug infusions, particularly when prompt establishment of the drug effect is critical.
A Canadian survey of transfusion practices in critically ill patients. Transfusion Requirements in Critical Care Investigators and the Canadian Critical Care Trials Group.

OBJECTIVES To characterize the contemporary red cell transfusion practice in the critically ill and to define clinical factors that influence these practices. DESIGN Scenario-based national survey. STUDY POPULATION Canadian critical care practitioners. MEASUREMENTS AND MAIN RESULTS We evaluated transfusion thresholds before transfusion and the number of red cell units ordered, under the given conditions. Of 254 Canadian critical care physicians, 193 (76%) responded to the survey. The primary specialty of most respondents was internal medicine (56%). Internal medicine respondents were in practice for an average of 8.4 +/- 5.7 (SD) yrs, and worked most often in combined medical/surgical intensive care units. Baseline hemoglobin transfusion thresholds averaged from 8.3 +/- 1.0 g/dL in a scenario involving a young stable trauma victim to 9.5 +/- 1.0 g/dL for an older patient after gastrointestinal bleeding. Transfusion thresholds differed significantly (p< .0001) between all four separate scenarios. With the exception of congestive heart failure (p> .05), all clinical factors (including age, Acute Physiology and Chronic Health Evaluation II score, preoperative status, hypoxemia, shock, lactic acidosis, coronary ischemia, and chronic anemia) significantly (p< .0001) modified the transfusion thresholds. A statistically significant (p< .01) difference in baseline transfusion thresholds was noted across four major regions (with a maximum of five academic centers per region) of the country. Low physician numbers in two of the regions did not allow for further investigation of regional variations. CONCLUSIONS There is significant variation in critical care transfusion practice, with many intensivists adhering to a 10.0-g/dL threshold, while other physicians described a much more restrictive approach to red cell transfusion. Also, many physicians opted to administer multiple units, despite published guidelines to the contrary. Additionally, the administration of red cells was strongly influenced by a number of clinical factors, many unique to intensive care unit patients. There is a need for prospective studies to define optimal practice in the critically ill.
Do race and gender influence the use of invasive procedures?

OBJECTIVE: To assess the influence of race and gender on the use of invasive procedures in patients with acute myocardial infarction (AMI) in community hospitals.DESIGN: Prospective, observational.SETTING: Five mid-Michigan community hospitals.PATIENTS: All patients (838) identified with AMI between January 1994 and April 1995 in 1 of these hospitals.MEASUREMENTS AND MAIN RESULTS: After adjusting for age, hospital of admission, insurance type, severity of AMI, and comorbidity, using white men as the reference group, the rate of being offered cardiac catheterization (CC) was 0.88 (95% confidence interval [CI], 0.60 to 1.29) for white women; 0.79 (95% CI, 0.41 to 1.50) for black men; and 1.14 (95% CI, 0.53 to 2.45) for black women. Among patients who underwent CC, after also adjusting for coronary artery anatomy, the rate of being offered angioplasty, using white men as the reference group, was 1.22 (95% CI, 0.75 to 1.98) for white women; 0.61 (95% CI, 0.29 to 1.28; P=.192) for black men; and 0.40 (95% CI, 0.14 to 1.13) for black women. The adjusted rate of being offered bypass surgery was 0.47 (95% CI, 0.24 to 0.89) for white women; 0.36 (95% CI, 0.12 to 1.06) for black men; and 0.37 (95% CI, 0.11 to 1.28) for black women.CONCLUSIONS: Our study shows that white women are less likely than white men to be offered bypass surgery after AMI. Although black men and women with AMI are less likely than white men to be offered percutaneous transluminal coronary angioplasty or coronary artery bypass grafting in both unadjusted and adjusted analyses, these findings did not reach statistical significance. Our study is limited in power due to the small number of blacks in the sample.
Multiple elements in the upstream glucokinase promoter contribute to transcription in insulinoma cells.

beta-cell type-specific expression of the upstream glucokinase promoter was studied by transfection of fusion genes and analysis of DNA-protein interactions. A construct containing 1,000 bp of 5'-flanking DNA was efficiently expressed in HIT M2.2. 2 cells, a beta-cell-derived line that makes both insulin and glucokinase, but not in NIH 3T3 cells, a heterologous cell line. In a series of 5' deletion mutations between bases -1000 and -100 (relative to a base previously designated +1), efficient expression in HIT cells was maintained until -280 bp, after which transcription decreased in a stepwise manner. The sequences between -180 and -1 bp contributing to transcriptional activity in HIT cells were identified by studying 28 block transversion mutants that spanned this region in 10-bp steps. Two mutations reduced transcription 10-fold or more, while six reduced transcription between 3- and 10-fold. Three mutationally sensitive regions of this promoter were found to bind to a factor that was expressed preferentially in pancreatic islet beta cells. The binding sites, designated upstream promoter elements (UPEs), shared a consensus sequence of CAT(T/C)A(C/G). Methylation of adenine and guanine residues within this sequence prevented binding of the beta-cell factor, as did mutations at positions 2, 3, and 5. Analysis of nuclear extracts from different cell lines identified UPE-binding activity in HIT M2.2. 2 and beta-TC-3 cells but not in AtT-20, NIH 3T3, or HeLa cells; the possibility of a greatly reduced amount in alpha-TC-6 cells could not be excluded. UV laser cross-linking experiments supported the beta-cell type expression of this factor and showed it to be approximately 50 kDa in size. Gel mobility shift competition experiments showed that this beta-cell factor is the same that binds to similar elements, termed CT boxes, in the insulin promoter. Thus, a role for these elements (UPEs or CT boxes), and the beta-cell factor that binds to them, in determining the expression of genes in the beta cells of pancreatic islets is suggested.
Lateral Diffusion of Inositol 1,4,5-Trisphosphate Receptor Type 1 Is Regulated by Actin Filaments and 4.1N in Neuronal Dendrites*

Inositol 1,4,5-trisphosphate receptor type1 (IP3R1) plays an important role in neuronal functions; however, the lateral diffusion of IP3R1 on the endoplasmic reticulum membrane and its regulation in the living neurons remain unknown. We expressed green fluorescent protein-tagged IP3R1 in cultured rat hippocampal neurons and observed the lateral diffusion by the fluorescence recovery after photobleaching technique. IP3R1 showed lateral diffusion with an effective diffusion constant of ∼0.3 μm2/s. Depletion of actin filaments increased the diffusion constant of IP3R1, suggesting that the diffusion of IP3R1 is regulated negatively through actin filaments. We also found that protein 4.1N, which binds to IP3R1 and contains an actin-spectrin-binding region, was responsible for this actin regulation of the IP3R1 diffusion constant. Overexpression of dominant-negative 4.1N and blockade of 4.1N binding to IP3R1 increased the IP3R1 diffusion constant. The diffusion of IP3R type 3 (IP3R3), one of the isoforms of IP3Rs lacking the binding ability to 4.1N, was not dependent on actin filaments but became dependent on actin filaments after the addition of a 4.1N-binding sequence. These data suggest that 4.1N serves as a linker protein between IP3R1 and actin filaments. This actin filament-dependent regulation of IP3R1 diffusion may be important for the spatiotemporal regulation of intracellular Ca2+ signaling.