Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence of metabolic status and genetic merit for fertility on proteomic composition of bovine oviduct fluid†

Abstract The oviduct plays a crucial role in fertilization and early embryo development providing the microenvironment for oocyte, spermatozoa, and early embryo. Since dairy cow fertility declined steadily over the last decades, reasons for early embryonic loss have gained increasing interest. Analyzing two animal models, this study aimed to investigate the impact of genetic predisposition for fertility and of metabolic stress on the protein composition of oviduct fluid. A metabolic model comprised maiden Holstein heifers and postpartum lactating (Lact) and non-lactating (Dry) cows, while a genetic model consisted of heifers from the Montbéliarde breed and Holstein heifers with low- and high-fertility index. In a holistic proteomic analysis of oviduct fluid from all groups using nano-liquid chromatography tandem-mass spectrometry analysis and label-free quantification, we were able to identify 1976 proteins, among which 143 showed abundance alterations in the pairwise comparisons within both models. Most differentially abundant proteins were revealed between low fertility Holstein and Montbéliarde (52) in the genetic model and between lactating and maiden Holstein (19) in the metabolic model, demonstrating a substantial effect of genetic predisposition for fertility and metabolic stress on the oviduct fluid proteome. Functional classification of affected proteins revealed actin binding, translation, and immune system processes as prominent gene ontology (GO) clusters. Notably, Actin-related protein 2/3 complex subunit 1B and the three immune system-related proteins SERPIND1 protein, immunoglobulin kappa locus protein, and Alpha-1-acid glycoprotein were affected in both models, suggesting that abundance changes of immune-related proteins in oviduct fluid play an important role for early embryonic loss. Summary Sentence Protein composition of oviduct fluid—the natural environment for the early embryo—depends on metabolic status and genetic predisposition of dairy cows, affecting, among others, proteins related to immune response.
Comparison of Clinical Outcomes: Bivalirudin With Transfemoral Access Versus Heparin With Transradial Access in Patients With ST segment Elevation Myocardial Infarction.

INTRODUCTION The best combination of access site and anticoagulant used during primary percutaneous coronary intervention (PCI) in patients presenting with ST segment elevation myocardial infarction is not known. METHODS We conducted a retrospective cohort study of all patients >18 years of age who underwent primary PCI in 2 large regional ST segment elevation myocardial infarction centers in Massachusetts between 2012 and 2014. The cohort was divided into 3 groups: bival/fem, hep/rad, or off-protocol, based on anticoagulation and access used. We used multiple logistic regression model to compare major cardiovascular events-major adverse cardiovascular events (MACE) and bleeding complications between the 2 on-protocol groups (bival/fem and hep/rad). RESULTS Of the 1074 patients in this study, there were 443 (41%), 501 (47%), and 130 (12%) patients in bival/fem, hep/rad, and off-protocol groups, respectively. There were significantly higher number of cardiogenic shock patients in the bival/fem compared to the hep/rad group (6.5% vs. 3.0%, P < 0.001). There was a trend toward reduced MACE in the hep/rad group compared to bival/fem (2.8 % vs. 5.1%, P = 0.068). When cardiogenic shock patients are excluded, there is no significant difference in mortality rates (bival/fem: 2.7% vs. hep/rad: 1.0%, P = 0.07) or bleeding complications between the groups (hep/rad: 4.5% vs. bival/fem: 2.1%, P = 0.06). CONCLUSIONS In patients undergoing primary PCI, there was a trend toward reduced inpatient MACE with the use of heparin and radial access compared with bivalirudin with femoral access. In patients without cardiogenic shock, there is no significant difference in mortality or bleeding rates between the 2 groups.
Design of the Squared Daisy: A Multi-Mode Energy Harvester, with Reduced Variability and a Non-Linear Frequency Response

With the rise of the Internet of Things (IoT) and the ever-increasing number of integrated sensors, the question of powering these devices represents an additional challenge. The traditional approach is to use a battery; however, harvesting energy from the environment seems to be the most practical approach. To that end, the use of piezoelectric MEMS energy has been proven as a potential power source in a wide range of applications. In this work, a proof of concept for a new architecture for MEMS energy harvesters is presented. The influence of the dimensions and different characteristics of these designs is discussed. These designs have been proven to be resilient to process variation thanks to their unique architecture. This work presents the use of vibration enhancement petals in order to widen the bandwidth of the energy harvester and provide a non-linear frequency response. The use of these vibration enhancement petals has allowed the fabrication of three design variations, each using an area of 1700 µm by 1700 µm. These designs have an operating bandwidth between 3.9 kHz and 14.5 kHz and can be scaled to achieve other targeted resonant frequencies.
Challenges to pooling models of crowding: Implications for visual mechanisms

A set of phenomena known as crowding reveal peripheral vision's vulnerability in the face of clutter. Crowding is important both because of its ubiquity, making it relevant for many real-world tasks and stimuli, and because of the window it provides onto mechanisms of visual processing. Here we focus on models of the underlying mechanisms. This review centers on a popular class of models known as pooling models, as well as the phenomenology that appears to challenge a pooling account. Using a candidate high-dimensional pooling model, we gain intuitions about whether a pooling model suffices and reexamine the logic behind the pooling challenges. We show that pooling mechanisms can yield substitution phenomena and therefore predict better performance judging the properties of a set versus a particular item. Pooling models can also exhibit some similarity effects without requiring mechanisms that pool at multiple levels of processing, and without constraining pooling to a particular perceptual group. Moreover, we argue that other similarity effects may in part be due to noncrowding influences like cuing. Unlike low-dimensional straw-man pooling models, high-dimensional pooling preserves rich information about the stimulus, which may be sufficient to support high-level processing. To gain insights into the implications for pooling mechanisms, one needs a candidate high-dimensional pooling model and cannot rely on intuitions from low-dimensional models. Furthermore, to uncover the mechanisms of crowding, experiments need to separate encoding from decision effects. While future work must quantitatively examine all of the challenges to a high-dimensional pooling account, insights from a candidate model allow us to conclude that a high-dimensional pooling mechanism remains viable as a model of the loss of information leading to crowding.
Targeting HSP90 attenuates angiotensin II-induced adventitial remodeling via suppression of mitochondrial fission.

AIMS Adventitial remodeling presenting with the phenotypic switch of adventitial fibroblasts (AFs) to myofibroblasts (MFs) is reportedly involved in the evolution of several vascular diseases, including hypertension. In our previous study, we reported that heat shock protein 90 (HSP90) inhibition by 17-dime-thylaminoethylamino-17-demethoxygeldanamycin (17-DMAG) markedly attenuates angiotensin II (AngII)-induced abdominal aortic aneurysm formation by simultaneously inhibiting several key signaling and transcriptional pathways in vascular smooth muscle cells (VSMCs); however, little is known about its role on AFs. Given that the AF phenotypic switch is likely to be associated with mitochondrial function and calcineurin, a client protein of HSP90 that mediates mitochondrial fission and function, the aim of this study was to investigate whether mitochondrial fission contributes to phenotypic switch of AF, and if it does, we further aimed to determine whether HSP90 inhibition attenuates mitochondrial fission and subsequently suppresses AF transformation and adventitial remodeling in AngII-induced hypertensive mice. METHODS AND RESULTS In primary mouse AFs, we found that calcineurin-dependent dephosphorylation of Drp1 induced mitochondrial fission and regulated mitochondrial ROS production, which stimulated AF proliferation, migration, and phenotypic switching in AngII-treated AFs. Moreover, AngII was found to increase the binding of HSP90 and calcineurin in AFs, while HSP90 inhibition significantly reversed AngII-induced mitochondrial fission and AF phenotypic switching by modulating the calcineurin-dependent dephosphorylation of Drp1. Consistent with the effects in AFs, in an animal model of AngII-induced adventitial remodeling, 17-DMAG markedly reduced mitochondrial fission, AF differentiation, vessel wall thickening, and fibrosis in the aortic adventitia, which were mediated by calcineurin/Drp1 signaling pathways. CONCLUSIONS Our study suggests that calcineurin/Drp1-dependent mitochondrial fission may be essential for understanding adventitial remodeling in hypertension and that HSP90 inhibition may serve as a novel approach for the treatment of adventitial remodeling-related diseases.
Gestational Weight Gain and Severe Maternal Morbidity at Delivery Hospitalization.

Financial Disclosure: Dr. Burke is an employee of Modern Fertility and has stock options in the company. Dr. Beqaj is a paid consultant and serves as a scientific advisor for Modern Fertility. Dr. Beqaj is a technical consultant for U.S. Specialty Labs, which performed the laboratory testing for the data presented in this report under the direction of the authors. Dr. Beqaj is also the laboratory director of Universal Diagnostic Labs, which measures reproductive hormones. Dr. Douglas is the chair of Modern Fertility’s medical advisory board, is a paid consultant of Modern Fertility, and has stock options in the company. Dr. Douglas measured reproductive hormones in her clinical practice as a Reproductive Endocrinologist at Columbia University Medical Center (50% effort, 2015–April 30, 2018) and at Rutgers-New Jersey Medical School (20% effort, May 1, 2018–present) and bills these tests. Dr. Douglas is funded by NIH grant R01 HL127013-01A1, entitled “Role of Angiogenic Notch in Uterine Decidualization and Placentation. ”
Methods for overcoming barriers in palliative care for ethnic/racial minorities: a systematic review

Abstract Objectives Ethnic/racial minority groups are less likely to discuss issues involving end-of-life treatment preferences and utilize palliative care or hospice services. Some barriers may be differences in language, religion, lower levels of health literacy, or less access to healthcare services and information. The purpose of this article is to conduct a systematic review on interventional studies that investigated methods to overcome the barriers faced by ethnic/racial minorities when accessing end-of-life services, including completing advanced directives, accepting palliative care, and enrolling in hospice. Methods Literature searches using four standard scientific search engines were conducted to retrieve articles detailing original research in an interventional trial design. All studies were conducted in an outpatient setting, including primary care visits, home visits, and dialysis centers. Target populations were those identified from ethnic or racial minorities. Results Nine articles were selected to be included in the final review. All were full-text English language articles, with target populations including African Americans, Hispanic or Latinos, and Asian or Pacific Islanders. Measured outcomes involved level of comfort in discussing and knowledge of palliative care services, desire for aggressive care at the end-of-life, completion of advance directives, and rate of enrollment in hospice. Significance of Results Three main avenues of interventions included methods to enhance patient education, increase access to healthcare, or improve communication to establish better rapport with target population. Studies indicate that traditional delivery of healthcare services may be insufficient to recruit patients from ethnic/racial minorities, and outcomes can be improved by implementing tailored interventions to overcome barriers.
Modeling nitrogen and phosphorus export with InVEST model in Bosten Lake basin of Northwest China

Bosten Lake is an important region of Northwest China that has transformed from a freshwater lake to a saltwater lake since the 1970s. The water quality in the Bosten Lake basin is important for social and economic development, and nitrogen (N) and phosphorus (P) are the key indicators of water quality. The land use data, precipitation data and Digital Elevation Model (DEM) data with the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model were used to simulate the N and P exports of the Bosten Lake basin. The spatial and temporal dynamics of nitrogen and phosphorus exports, and the response of nitrogen and phosphorus exports to land use change and precipitation change were analyzed between 2000 and 2015. The results show that the amount of N and P exports increased during 2000–2015, and the N and P exports are mainly distributed around Bosten Lake. The N and P exports are greatly affected by cultivated land, built-up areas and grassland, while they are less affected by other land use types. The high precipitation areas with small exports of N and P are mainly distributed in mountain areas, while small precipitation areas with large exports of N and P are distributed in plains where the cultivated land and built-up areas are concentrated. The InVEST model can be used in Northwest China, and the statistical downscaling of reanalysis precipitation data can be used in the InVEST model to improve the simulation accuracy in the data scarce regions of Northwest China.
Clinicoradiological study of adult Chiari malformation type 1 patients with emphasis on cerebrospinal fluid peak flow velocity at foramen magnum level

Introduction: The aim of this study was to determine the peak cerebrospinal fluid (CSF) flow velocity at the foramen magnum level in adult patients with Chiari type 1 malformation (CM1) and to determine the changes in velocity after posterior fossa decompression. An attempt was also made to determine whether or not CSF flow velocity can be a significant predictor in patients who need surgical intervention. Materials and Methods: A prospective longitudinal study was conducted in 32 symptomatic patients of CM1 treated with craniocervical decompression. Only adult patients with age ≥18 years and tonsillar herniation ≥5 mm were included in this study. Clinical and radiological assessment of patients with reference to their CSF flow characteristics was done both preoperatively and after suboccipital decompression. Results: Out of the 32 patients, 30 patients underwent a suboccipital decompression and two patients were treated with a venriculoperitoneal shunt procedure due to gross hydrocephalus. The preoperative mean tonsillar herniation was 10.4 ± 4.64 mm that reduced to 7.35 ± 3.10 mm in the follow up period. Postoperatively, there was also a substantial decrease in the peak CSF velocity at the foramen magnum along with reduction in the extent and size of the syrinx. These changes in CSF velocity correlated with a more normal appearing foramen magnum and an improvement in symptoms. Conclusion: Although the selection criteria for surgery are based mainly on the degree of tonsillar ectopia and presenting symptoms, the degree of CSF flow obstruction rather than the degree of tonsillar herniation can better select patients who are most responsive to surgery. An improved CSF velocity profile following surgery in such patients is a useful guide to anticipate a symptomatic improvement.
Factors associated with atypical radiological findings of pulmonary tuberculosis

Background Unusual radiological images may delay diagnosis of pulmonary tuberculosis. This study aimed to analyze the risk factors for an atypical radiological image in patients with pulmonary tuberculosis. Methods We retrospectively analyzed data from patients admitted to one hospital from January 2013 to December 2016 for sputum smear-positive lung tuberculosis who underwent chest computed tomography (CT) on admission. Patients whose sputum cultures were positive for general bacteria were excluded. Patient characteristics and laboratory data were compared between patients with cavity and those without and between patients with upper predominant lung involvement and those without. Results This study included 94 (93%) of 101 patients who underwent chest CT. The non-cavity group was older, had a greater number of females, had a lower C-reactive protein (CRP) level, and had a lower glomerular filtration rate. Multivariate analysis showed that a low CRP level (OR 0.808; 95% CI 0.674–0.967; p = 0.020) significantly predicted non-cavity pulmonary tuberculosis. The non-upper predominant lung involvement group was older and had a greater number of females, poorer performance status, a higher CRP level, and a lower serum albumin level. A poor performance status (OR 2.155; 95% CI 1.257–3.693; p = 0.005) was found to significantly predict pulmonary tuberculosis with non-upper predominant lung distributions. Conclusions A low CRP level and poor performance status were associated with non-cavity and non-upper predominant lung distribution, respectively, in patients with pulmonary tuberculosis. Tuberculosis patients with these characteristics may present unusual chest images.
Effects of dietary rapeseed meal inclusion levels on growth performance, organ weight, and serum biochemical parameters in Cherry Valley ducks

The aim of this study was to investigate the effects of the inclusion levels of different types of rapeseed meal (RSM) on performance, organ weight, and serum biochemical parameters in Cherry Valley ducks in the starter period and grower-finisher period. In Exp. 1, a total of 750 seven-day-old male ducklings were divided into 5 dietary treatments with 6 replicate pens of 25 birds per pen. The starter diets with the inclusion of 0, 5, 10, 15, or 20% of double-low RSM contained 0, 1.37, 2.15, 3.46, or 5.31 µmol glucosinolates (GLS)/g in the finished feed (from day 7 to 21). In Exp. 2, a total of 900 fifteen-day-old male ducklings were divided into 6 dietary treatments with 6 replicate pens of 25 birds per pen. The grower-finisher diets with the inclusion of 0, 5, 10, 15, 20, or 25% of Indian RSM contained 0, 7.67, 15.34, 24.66, 31.21, or 38.44 µmol GLS/g in the finished feed (from day 15 to 42). For ducklings in the starter period (Exp. 1), body weight gain and feed intake decreased linearly as the dietary double-low RSM inclusion level increased at day 7 to 14, while growth rate was not influenced by dietary double-low RSM inclusion levels at day 15 to 21 and day 7 to 21. For ducks in the grower-finisher period (Exp. 2), growth performance decreased linearly as the dietary RSM inclusion level increased from 5 to 20%. In addition, dietary RSM inclusion levels induced liver enlargement in ducklings at day 21 (5 to 20% double-low RSM with 1.37 to 5.31 µmol/g GLS) and thyroid enlargement accompanied by increased serum AST and ALP activities in ducks at day 42 (5 to 15% Indian RSM with 7.67 to 23.66 µmol/g GLS). Therefore, our results indicated that the upper limit of using RSM sources in feed formulation should consider the anti-nutritional factor of GLS content at different stages of duck growth.
Molecular Profiling of Long-Term IDH-wildtype Glioblastoma Survivors.

BACKGROUND Glioblastoma (GBM) represents an aggressive cancer type with a median survival of only 14 months. With fewer than 5% of patients surviving five years, comprehensive profiling of these rare patients could elucidate prognostic biomarkers that may confer better patient outcomes. We utilized multiple molecular approaches to characterize the largest patient cohort of long-term IDH-wildtype GBM survivors (LTS) to date. METHODS Retrospective analysis was performed on 49 archived formalin-fixed paraffin embedded tumor specimens from patients diagnosed with GBM at the Mayo Clinic between December 1995 and September 2013. These patient samples were subdivided into two groups based on survival (12 LTS, 37 short-term survivors (STS)) and subsequently examined by mutation sequencing, copy number analysis, methylation profiling, and gene expression. RESULTS Of the 49 patients analyzed in this study, LTS were younger at diagnosis (p=0.016), more likely to be female (p=0.048), and MGMT promoter methylated (UniD, p=0.01). IDH-wildtype STS and LTS demonstrated classic GBM mutations and copy number changes. Pathway analysis of differentially expressed genes showed LTS enrichment for sphingomyelin metabolism, which has been linked to decreased GBM growth, invasion, and angiogenesis. STS enriched for DNA repair and cell cycle control networks. CONCLUSIONS While our findings largely report remarkable similarity between these LTS and more typical STS, unique attributes were observed in regard to altered gene expression and pathway enrichment. These attributes may be valuable prognostic markers and are worth further examination. Importantly, this study also underscores the limitations of existing biomarkers and classification methods in predicting patient prognosis.
The Basic Psychological Need Satisfaction and Frustration Scale in Exercise (BPNSFS-E): Validity, Reliability, and Gender Invariance in Portuguese Exercisers

The aim of this study was to translate and validate the Basic Psychological Need Satisfaction and Frustration Scale for Portuguese exercisers. In addition, we sought to analyze gender invariance. We collected data from two independent samples of Portuguese respondents—a calibration sample with 316 participants and a validation sample with 632 participants. Results from confirmatory factor analysis supported the original six-factor model in both the calibration sample, χ2(237) = 471.814, χ2/df = 1.99; B–S p < . 001, Comparative Fit Index = . 935, Tucker–Lewis Index =. 924, standard mean root square residual = . 047, root mean square error of approximation = . 057 (90% confidence interval = [.050, .065]), and the validation sample, χ2(237) = 571.796, χ2/df = 2.41; B–S p < . 001, Comparative Fit Index = . 948, Tucker–Lewis Index = . 940, standard mean root square residual = . 038, root mean square error of approximation = . 047 (90% confidence interval = [.042, .052]). Moreover, our analysis revealed acceptable internal consistency, convergent and discriminant validity of the translated version, and invariance between the two samples and between genders, as differences across latent means showed that magnitude effects were trivial between samples and between male and female exercisers. These results support the use of the adapted scale among both male and female exercisers.