Edge computing in IoT : Preliminary results on modeling and performance analysis

Publications[120] V. Borutinskait , G. Treigyt , D. Matuzevi ius, V. eksteryt , B. Kurtinaitien , A. Serackis, D. Navakauskas, and R. Navakauskien . Proteomic studies of honeybeeand manually-collected pollen. Zemdirbyste-Agriculture, 106(2):183190, 2019. [ bib | DOI | www: | . pdf ] In this study a new approach to quantitative and qualitative proteomic evaluation of pollen of different botanical origin was proposed. A monofloral mixture of plum (Prunus) 86.83 +/0.70% and willow (Salix spp.) 13.7 +/0.30% pollen, and monofloral plum (Prunus) pollen collected by bees was studied. Other pollen was collected manually from pear (Pyrus communis), apple tree (Malus sylvestris), cherry (Prunus / Cerasus) and wild cherry (Prunus avium). Samples were made during an early spring plant blossom. Proteins isolated from pollen samples were fractionated by a two-dimensional electrophoresis (2-DE) and about 50 proteins were identified using mass spectrometry analysis. A three-dimensional (3-D) visualization of proteins was performed. The intensities of protein spots differed in the orchard pollen samples more than ten times. The biological functions of the identified proteins differ, i.e. they are involved in transcription / translation, metabolic and other cellular processes. The data revealed up-regulated process of the enzyme 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1 in all pollen involved in methionine formation, which was the highest in monofloral orchard pollen collected by bees. This enzyme was identified in all the tested pollen. High level of expression of putative flavin-containing monooxygenase FMO GS-OX-like 11 protein was found in manually-collected pollen. This protein is important for plant protection against pathogens as well for plant hormone biosynthesis. [119] V. Ceksteryte, V. Borutinskaite, D. Matuzevicius, G. Treigyte, D. Navakauskas, B. Kurtinaitiene, and R. Navakauskiene. Evaluation of proteome profiles of salix spp. pollen and relationship between glucose oxidase activity and pollen content in willow honey. Baltic Forestry, 25(1):83-96, 2019. [ bib | www: | . pdf ] Proteins from handand bee-collected pollen were isolated and separated in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2DE) system as well as by direct gel-free mass spectrometry (MS) analysis. Total thirty-six plant proteins with known functions and three uncharacterized were identified using mass spectrometry (MS) techniques from fourteen protein spots separated in 2D gel electrophoresis. Our study revealed two forms of profilin proteins with slightly different mass of 14.19 kDa and 14.09 kDa and beta actin fragment with 33.25 kDa both in handand bee-collected pollen as well proteins of thioredoxin family with molecular weight of 47.29 kDa. The antioxidant enzyme glucose oxidase (GOX) activity was investigated in twelve samples of monofloral willow honey, having Salix spp. pollen from 44.7% to 80.2%. Data of paired regression analysis between Salix spp. pollen content and GOX activity show moderate and negative correlation coefficient (r = -0.56). [118] M. Gasiuniene, G. Petkus, D. Matuzevicius, D. Navakauskas, and R. Navakauskiene. Angiotensin ii and tgf-beta 1 induce alterations in human amniotic fluid-derived mesenchymal stem cells leading to cardiomyogenic differentiation initiation. International Journal of Stem Cells, 12(2):251-264, 2019. [ bib | DOI | www: | . pdf ] Background and Objectives: Human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) may be a valuable source for cardiovascular tissue engineering and cell therapy. The aim of this study is to verify angiotensin II and transforming growth factor-beta 1 (TGF-? 1) as potential cardiomyogenic differentiation inducers of AFMSCs. Methods and Results: AF-MSCs were obtained from amniocentesis samples from second-trimester pregnant women, isolated and characterized by the expression of cell surface markers (CD44, CD90, CD105 positive; CD34 negative) and pluripotency genes (OCT4, SOX2, NANOG, REX1). Cardiomyogenic differentiation was induced using different concentrations of angiotensin II and TGF-? 1. Successful initiation of differentiation was confirmed by alterations in cell morphology, upregulation of cardiac genes-markers NKX2-5, TBX5, GATA4, MYH6, TNNT2, DES and main cardiac ion channels genes (sodium, calcium, potassium) as determined by RT-qPCR. Western blot and immunofluorescence analysis revealed the increased expression of Connexin43, the main component of gap junctions, and Nkx2.5, the early cardiac transcription factor. Induced AF-MSCs switched their phenotype towards more energetic and started utilizing oxidative phosphorylation more than glycolysis for energy production as assessed using Agilent Seahorse XF analyzer. The immune analysis of chromatin-modifying enzymes DNMT1, HDAC1/2 and Polycomb repressive complex 1 and 2 (PRC1/2) proteins BMI1, EZH2 and SUZ12 as well as of modified histones H3 and H4 indicated global chromatin remodeling during the induced differentiation. Conclusions: Angiotensin II and TGF-? 1 are efficient cardiomyogenic inducers of human AF-MSCs; they initiate alterations at the gene and protein expression, metabolic and epigenetic levels in stem cells leading towards cardiomyocyte-like phenotype formation. [117] D. Navakauskas and R. Pupeikis. On-line approach for fast convolution over sensor networks. Tem Journal-Technology Education Management Informatics, 7(2):243-249, 2018. [ bib | DOI | www: | . pdf ] It is assumed that at some time moment, in wireless sensor networks the new set of current samples of input and system impulse response enter a digital memory replacing the previous samples. It is urgent for each new sample or for a small part of new samples to update a convolution as well. Therefore, a recursive fast convolution algorithm is proposed here to solve a linear filtering problem for a nonstationary system. The calculation operations are reduced because most columns of Fourier code matrices and respective rows of the right-hand side vectors were deleted for equal previous and current samples. An example with the ordinary and modified 8-point Fourier code matrices is presented for a nonstationary linear system. The amounts of operations, necessary for recursive and fast Fourier transform algorithms, are calculated. Results are discussed, and the conclusion is given. [116] Julius Skirelis and Dalius Navakauskas. Classifier based gateway for edge computing. In Proceedings of the 6th IEEE Workshop on Advances in Information, Electronic and Electrical Engineering, AIEEE 2018, pages 1-4. IEEE, 2018. [ bib | . pdf ] Edge Computing technology aims to replace regular cloud IoT solutions on applications where data intensity and link latency plays critical role. Improvement is achieved by placing processing at the edge of the network, deploying service close to data source of user. Limited resources of Edge devices stipulate the need to smartly distribute over Dalius Navakauskas