Miracle-dz/UniFAR-FTUs huggingface dataset:
Biomechanical Evaluation on Balance During Standing on Various Height and Contact Area Heeled Shoes

People who live in urban cities need comfortable shoes which can support various daily activities and give attractive appearance. In women society, shoes have high aesthetic values because they are considered improve appearance and enhance confidence. Contrast with attractive appearance, high heeled shoes give negative effects on health. High heeled shoes could reduce: muscle movement around ankle, total foot movement, and balance control that may cause injury. This research is purposed to measure biomechanical phenomenon on balance when wearing high heels with various type of height and contact area. Seven ladies (age: 18–21 years old, weight, 50-55kg, height: 148–157 cm, shoes size: 38) - who didn't wear high-heeled shoes for daily activities - were chosen to become the subjects in the experiment. Each subject was asked to stand on bare foot and wore 11 types of shoes with various height (low, mid, high, very high) and base support (pointed, chunky, wedges). Balance assessment was done using force plate. They stood on dominant foot, while the other foot was elevated for 6 seconds. Stability test showed that the most unstable shoes is pointed high, followed by chunky, and the most stable is wedges high. Stability decrease as the contact area gets smaller and the shank curve angle and wedge angle get steeper. The instability is higher in medial-lateral direction than antero posterior direction.
Antigen Specificity and Clinical Significance of IgG and IgA Autoantibodies Produced in situ by Tumor-Infiltrating B Cells in Breast Cancer

An important role for tumor infiltrating B lymphocytes (TIL-B) in the immune response to cancer is emerging; however, very little is known about the antigen specificity of antibodies produced in situ. The presence of IgA antibodies in the tumor microenvironment has been noted although their biological functions and clinical significance are unknown. This study used a 91-antigen microarray to examine the IgG and IgA autoantibody repertoires in breast cancer (BC). Tumor and adjacent breast tissue supernatants and plasma from BC patients together with normal breast tissue supernatants and plasma from healthy controls (patients undergoing mammary reduction and healthy blood donors) were analyzed to investigate relationships between autoantibodies and the clinical, histological and immunological features of tumors. Our data show that >84% of the BC samples tested contain autoantibodies to one or more antigens on the array, with ANKRD30BL, COPS4, and CTAG1B being most frequently reactive. Ex vivo TIL-B responses were uncoupled from systemic humoral responses in the majority of cases. A comparison of autoantibody frequencies in supernatants and plasma from patients and controls identified eight antigens that elicit BC-associated autoantibody responses. The overall prevalence of IgG and IgA autoantibodies was similar and while IgG and IgA responses were not linked they did correlate with distinct clinical, pathological and immunological features. Higher levels of ex vivo IgG responses to BC-associated antigens were associated with shorter recurrence-free survival (RFS), HER2 overexpression and lower tumor-infiltrating CD8+ T cell counts. Higher IgA levels were associated with estrogen and progesterone receptor-negative cancers but were not significantly associated with RFS. Furthermore, ex vivo IgA but not IgG autoantibodies reactive to BC-associated antigens were linked with germinal center and early memory B cell maturation and the presence of tertiary lymphoid structures suggesting that these TIL-B are activated in the tumor microenvironment. Overall, our results extend the current understanding of the antigen specificity, the biological and the clinical significance of IgG and IgA autoantibodies produced by BC TIL-B in situ.
Redox- and Ligand Binding-Dependent Conformational Ensembles in the Human Apoptosis-Inducing Factor Regulate Its Pro-Life and Cell Death Functions.

Aims: The human apoptosis-inducing factor (hAIF) supports OXPHOS biogenesis and programmed cell death, with missense mutations producing neurodegenerative phenotypes. hAIF senses the redox environment of cellular compartments, stabilizing a charge transfer complex (CTC) dimer that modulates the protein interaction network. In this context, we aimed to evaluate the subcellular pH, CTC formation, and pathogenic mutations effects on hAIF stability, and a thermal denaturation high-throughput screening (HTS) assay to discover AIF binders. Results: Apoptotic hAIFΔ1-101 is not stable at intermembrane mitochondrial space (IMS) pH, but the 77-101 residues confer stability to the mitochondrial isoform. hAIF and its CTC populate different conformational ensembles with redox switch to the CTC producing a less stable and compact protein. The pathogenic G308E, ΔR201, and E493V mutations modulate hAIF stability; particularly, ΔR201 causes a population shift to a less stable conformation that remodels active site structure and dynamics. We have identified new molecules that modulate the hAIF reduced nicotinamide adenine dinucleotide (NADH)/oxidized nicotinamide adenine dinucleotide (NAD+) association/dissociation equilibrium and regulate its catalytic efficiency. Innovation: Biophysical methods allow evaluating the regulation of hAIF functional ensembles and to develop an HTS assay to discover small molecules that might modulate hAIF stability and activities. Conclusions: The mitochondrial soluble 54-77 portion stabilizes hAIF at the IMS pH. NADH-redox-linked conformation changes course with strong NAD+ binding and protein dimerization, but they produce a negative impact in overall hAIF stability. Loss of functionality in the R201 deletion is due to distortion of the active site architecture. We report molecules that may serve as leads in the development of hAIF bioactive compounds.
Satisfied With Less? Mismatch Between Subjective and Objective Position of Immigrants and Native-Born Men and Women in the Labor Market

The current study introduces a new mismatch concept in labor studies, the mismatch between subjective work perceptions and actual labor market position, and examines it from the perspectives of gender, ethnic and migration. This mismatch, positive or negative, was examined among men and women from different ethnic groups in Israel, both immigrants and native-born. The analyses were conducted on 9,923 employees using the Israeli CBS Social Surveys (2013-2015). The results reveal that the gender effect is more prominent than the ethnicity and migration effect. In general, women were more satisfied with their actual position in the labor market (positive subjective mismatch) than men, and men were less satisfied with their actual position in the labor market (negative subjective mismatch) than women. A positive subjective mismatch was also found among men from disadvantaged ethnic and immigrant groups. The multivariate analyses revealed that after controlling for socio-economic variables, ethnic differences declined among both men and women. Possible explanations are discussed, primarily based on the notion of relative well-being in respect to workers' expectations.
Family History of Diabetes Is Associated With Delayed Fetal Postprandial Brain Activity

Introduction: We have previously shown that fetuses of mothers with gestational diabetes mellitus (GDM) and insulin resistance exhibit a prolongation of fetal auditory event-related brain responses (fAER) compared to fetuses of normal glucose tolerant women during an oral glucose tolerance test (oGTT). This implies that maternal metabolism may program the developing fetal brain. We now asked whether a family history of type 2 diabetes without metabolic programing also impacts fetal brain activity. We therefore investigated brain activity in fetuses of normal glucose tolerant mothers with and without family history of type 2 diabetes (FHD+ and FHD–). Methods: A 75 g oGTT was performed in healthy pregnant women. Plasma glucose and insulin levels were measured after 0, 60, and 120 min. Each blood draw was preceded by fetal magnetoencephalographic (fMEG) recordings of fAER. From a group of 167 participants, a subsample of 52 metabolically healthy women, 37 with a negative, and 15 with a positive FHD (at least one first- or second-degree relative) was carefully selected based on the following inclusion criteria: inconspicuous pregnancy, no GDM, BMI 18.5–30 kg/m2, no preterm birth and at least two fMEG with detectable fetal responses during oGTT. Results: An ANOVA showed a significant interaction between fMEG measurement time during the oGTT and FHD on fAER latency [F(2) = 4.163, p = 0.018]. Fetuses of mothers with FHD+ had a prolonged fAER (273 ± 113 ms) compared to fetuses of mothers with FHD– (219 ± 69 ms) at 60 min during the oGTT [F(1) = 4.902, p = 0.032]. There were no significant differences in age, BMI before pregnancy, weight gain during pregnancy and gestational age between the groups. Maternal glucose levels and insulin sensitivity were also not significantly different. Discussion: In addition to the previously shown influence of maternal metabolism on fetal brain activity, maternal family history of diabetes (FHD) is also linked to fetal postprandial brain activity. This indicates that genetic and/or epigenetic factors modulate the postprandial brain response of the developing fetus.
Low-Complexity Detection for MIMO C-FBMC Using Orthogonal Approximate Message Passing

We propose a low-complexity and high-performance detector for multi-input multi-output circular filter bank multicarrier (C-FBMC) system on the basis of an orthogonal approximate message passing algorithm. Low complexity and high performance are realized through the use of a special structure of the modulation matrix and block diagonalization of the equivalent channel matrix in the frequency domain. After exploring the property of the C-FBMC modulation matrix in the frequency domain, we design an algorithm to replace the direct inversion of the matrix, thus reducing complexity considerably. The complexity per iteration of the proposed method is <inline-formula> <tex-math notation="LaTeX">${\mathcal {O}} (KMT \log _{2}M)$</tex-math></inline-formula>, where <inline-formula> <tex-math notation="LaTeX">$K$</tex-math></inline-formula>, <inline-formula><tex-math notation="LaTeX">$M$</tex-math> </inline-formula>, and <inline-formula><tex-math notation="LaTeX">$T$</tex-math></inline-formula> denote the number of subcarriers, subsymbols, and transmit antennas, respectively. Numerical simulations show that the proposed iterative algorithm is superior to conventional detectors in terms of performance and computational complexity.
Morphologic Overlap Between Inflammatory Myofibroblastic Tumor and IgG4-related Disease: Lessons From Next-generation Sequencing

Inflammatory myofibroblastic tumor (IMT), a locally aggressive neoplasm capable of metastasis, may show an immunoglobulin (Ig)G4-rich lymphoplasmacytic infiltrate. Prior reports suggest that storiform-fibrosis and obliterative phlebitis aid in the distinction of IMT from IgG4-related diseases. Herein, we highlight the morphologic overlap between the 2 diseases, and emphasize the importance of a multiplex fusion assay in the distinction of IgG4-related disease (IgG4-RD) from IMT. We identified 7 IMTs with morphologic and immunohistochemical features of IgG4-RD; 3 patients were originally diagnosed with IgG4-RD. Demographic, clinical and morphologic data was recorded. We also reevaluated 56 patients with IgG4-RD. We performed immunohistochemistry for IgG4, IgG, ALK, and ROS1. In situ hybridization for IgG4 and IgG was performed in selected cases. A multiplex next-generation sequencing–based RNA assay for gene fusions was performed to detect all known IMT-related gene fusions. All 7 IMTs showed a dense lymphoplasmacytic infiltrate and storiform-type fibrosis, with obliterative phlebitis noted in 3 cases. The neoplastic stromal cells constituted <5% of overall cellularity and stromal atypia was either absent or focal and mild. Elevated numbers of IgG4 positive cells and increased IgG4 to IgG ratio was identified in all cases. Four cases showed ALK related abnormalities: 3 fusions and one alternative transcription initiation; while 2 patients showed ROS1 and NTRK3 fusions. One tumor was negative for known IMT-related gene fusions. All 56 IgG4-RD cases were negative for ALK and ROS1 on immunohistochemistry; 6 cases were negative on the fusion assay. Highly inflamed IMTs are indistinguishable from IgG4-RD both histologically and on immunohistochemistry for IgG4. We advocate scrutinizing patients with presumptive single organ IgG4-RD for IMT and the diagnostic algorithm should include ALK and ROS1 immunohistochemistry and, in selected cases, a next-generation sequencing–based fusion assay that covers known IMT-associated gene fusions.
Metacognitive Monitoring of Text Comprehension: An Investigation on Postdictive Judgments in Typically Developing Children and Children With Reading Comprehension Difficulties

The ability to assess and monitor one’s own understanding of a written text is fundamental for learning and academic achievement. In the current paper, postdictive monitoring of text comprehension (i.e., the ability to judge the accuracy of responses previously given to a reading comprehension test) was investigated in both typically developing (TD) children and children with reading comprehension difficulties. Children from primary school (3rd to 5th grade) and secondary school (6th to 8th grade) participated in the study (N = 245). They were administered standardized tasks for reading comprehension, in which they had to read two texts and answer 12 multiple-choice questions after each text; subsequently, they had to provide postdictive judgments evaluating their performance: for each answer they had to select whether they judged it as correct, incorrect or whether they were uncertain. Two scores were calculated: Bias score, indicating the difference between metacognitive judgments of accuracy and actual performance; and Accurate estimation, indicating the sum of correct answers judged as “correct” and incorrect answers judged as “incorrect. ” Results showed that primary school children were more overconfident than secondary school children and made fewer Accurate estimations especially for “correct” responses. Furthermore, the consideration of a group of children with reading comprehension difficulties showed that these failures are linked to worse metacognitive monitoring ability of comprehension performance in comparison not only to age-matched controls but also to the TD group of third-graders. Implications for learning and achievement are discussed.
Pilot Testing of Peak Alpha Frequency Stability During Repetitive Transcranial Magnetic Stimulation

Over half of those diagnosed with post-traumatic stress disorder (PTSD) have comorbid major depressive disorder (MDD), and rates are even higher among military veterans. Transcranial magnetic stimulation (TMS) may be a safe and efficacious treatment for PTSD, both with and without comorbid MDD. Still, the mechanism of action of TMS is not fully understood, and it remains unclear which stimulation techniques (e.g., target regions, pulse strength/frequency, waveform) optimize treatment for these patients. Recent research indicated that a patient's unique individualized alpha frequency (IAF) may be used to guide brain stimulation treatment, and emerging data suggests that stimulation synchronized to the IAF may be efficacious for MDD. However, to our knowledge there are no studies to date that evaluate the stability of IAF over time in patients with comorbid PTSD and MDD. To this end, we used an eight-lead electroencephalography (EEG) system to record IAF before and after a course of TMS. Stimulation parameters were informed by prior studies of TMS for comorbid PTSD and MDD and included 5 Hz TMS to the left dorsolateral prefrontal cortex, at 120% of motor threshold, 3,000–4,000 pulses per session for up to 40 sessions. We tested whether IAF was changed with a course of TMS therapy and evaluated whether IAF predicted clinical outcomes. We observed no significant changes in IAF from baseline to post-treatment, and there was no relationship between IAF and clinical symptom change. These data demonstrate the stability of IAF with TMS and indicate its utility as a trait marker for future brain stimulation studies. This work does not support the use of IAF as predictor of clinical response to TMS as administered.
Evidence needed to understand gender identity: Commentary on Turban & Ehrensaft (2018)

Turban and Ehrensaft (2018) have provided a thoughtful review of transgender identity in children and adolescents, emphasizing the benefits of the affirmative approach in maintaining positive psychological health in transgender individuals. The review reveals significant gaps in our understanding of gender identity regarding its nature, development, plasticity, causes, and links with other aspects of gender, and of the long-term benefits and costs of childhood social transitions. A full understanding of transgender identity requires studying cisgender identity too, and recognition that gender identity is continuous, develops across time and is not synonymous with gender expression or variations in gender-typed characteristics. Considerable evidence is needed before we can be confident that we are providing optimal treatment for children who are gender variant.
Upper airway stimulation in obstructive sleep apnea improves glucose metabolism and reduces hedonic drive for food

Upper airway stimulation is a new and effective second‐line treatment for obstructive sleep apnea, but possible consequences on glucose metabolism and central regulation of food intake are unclear. Twenty patients were prospectively studied before and 12 months after obstructive sleep apnea treatment by upper airway stimulation. Respiratory parameters and daytime sleepiness were assessed to document effectiveness of treatment. Glucose metabolism was assessed by the oral glucose tolerance test, and hedonic versus homeostatic drive to eat was characterized. At 12 months, upper airway stimulation significantly improved measures of obstructive sleep apnea (all p < 0.01). Despite no change in body weight, fasting C‐peptide insulin resistance index (p = 0.01) as well as insulin and C‐peptide levels at 60 min during the oral glucose tolerance test (p < 0.02) were reduced. Hedonic drive to eat was strongly reduced (p < 0.05), while leptin and ghrelin remained unchanged (p > 0.15). Upper airway stimulation is effective in treatment of obstructive sleep apnea and improves glucose metabolism. Reduced hedonic drive to eat might contribute to these metabolic improvements. These promising findings are in need for long‐term controlled evaluation of metabolic sequelae of upper airway stimulation and to mechanistically evaluate the metabolic benefits of upper airway stimulation in patients with obstructive sleep apnea.
Adherence to instructions and fluctuation of force magnitude in cervical headgear therapy.

OBJECTIVES: To investigate how patients adhere to instructions and how force magnitude fluctuates and influences the use of cervical headgear (CHG) therapy. MATERIALS AND METHODS: In this controlled clinical trial, subjects (n = 40) were treated with CHG with light (L, 300 g) or heavy (H, 500 g) force. Patients were asked to wear CHG for 10 hours per day for 10 months (ie, during sleep), but the importance for treatment of wearing CHG also in the evening hours was emphasized. Adherence to instructions and force magnitude in CHG use were monitored by electronic module (Smartgear, Swissorthodontics, Switzerland). RESULTS: Force magnitude can be set at a certain level, L or H, even if great individual variability is seen in all subjects (0-900 g). Children in the L group used CHG longer per day than those in the H group (9.3 hours ±1.5 hours and 7.8 hours ± 2.1 hours, respectively, P = .002). During evening hours, CHG was used more ( P = .02) in the L group than in the H group. In both groups, CHG was used less in the evening hours during school breaks than in the evening hours during school ( P < .001). CONCLUSIONS: Children with lower force in CHG seem to adhere better to the instructions for CHG use. Daily rhythm also influences the time of appliance use regardless of force magnitude. The force can be set to a certain magnitude level, even though there is substantial individual variability.
Crystal structure and catalytic activity of the PPM1K N94K mutant

Protein Phosphatase Mg2+/Mn2+‐Dependent 1K (PPM1K),also named as PP2Cm or branched‐chain α‐ketoacid dehydrogenase complex phosphatase, is a member of the metal‐dependent phosphatase family and an important metabolic regulator. Single nucleotide polymorphisms (SNPs) in PPM1K contributing to protein functional defects have been found to be associated with numerous human diseases, such as cardiovascular disease, maple syrup urine disease, type 2 diabetes, and neurological disease. PPM1K N94K is an identified missense mutant produced by one of the SNPs in the human PPM1K coding sequence. However, the effects of the N94K mutant on its activity and structural property have not been defined. Here, we performed a detailed enzymological study using steady‐state kinetics in the presence of pNPP or phospho‐peptide substrates and crystallographic analyses of the wild‐type and N94K PPM1K. The PPM1K‐N94K significantly impaired its Mg2+‐dependent catalytic activity and structural analysis demonstrated that the N94K mutation induced a conformational change in the key residue in coordinating the Mg2+ in the active site. Specifically, three Mg2+ were located in the active site of the PPM1K N94K instead of two Mg2+ in the PPM1K wild type. Therefore, our results provide a structure basis for the metal ion‐dependent PPM1K‐N94K phosphatase activity.
A high‐quality chromosome‐level genome assembly of a generalist herbivore, Trichoplusia ni

The cabbage looper, Trichoplusia ni, is a globally distributed highly polyphagous herbivore and an important agricultural pest. T. ni has evolved resistance to various chemical insecticides, and is one of the only two insect species that have evolved resistance to the biopesticide Bacillus thuringiensis (Bt) in agricultural systems and has been selected for resistance to baculovirus infections. We report a 333‐Mb high‐quality T. ni genome assembly, which has N50 lengths of scaffolds and contigs of 4.6 Mb and 140 Kb, respectively, and contains 14,384 protein‐coding genes. High‐density genetic maps were constructed to anchor 305 Mb (91.7%) of the assembly to 31 chromosomes. Comparative genomic analysis of T. ni with Bombyx mori showed enrichment of tandemly duplicated genes in T. ni in families involved in detoxification and digestion, consistent with the broad host range of T. ni. High levels of genome synteny were found between T. ni and other sequenced lepidopterans. However, genome synteny analysis of T. ni and the T. ni derived cell line High Five (Hi5) indicated extensive genome rearrangements in the cell line. These results provided the first genomic evidence revealing the high instability of chromosomes in lepidopteran cell lines known from karyotypic observations. The high‐quality T. ni genome sequence provides a valuable resource for research in a broad range of areas including fundamental insect biology, insect‐plant interactions and co‐evolution, mechanisms and evolution of insect resistance to chemical and biological pesticides, and technology development for insect pest management.