Miracle-dz/UniFAR-FTUs huggingface dataset:
How well do muscle biomechanics predict whole-animal locomotor performance? The role of Ca2+ handling

SUMMARY It is important to determine the enabling mechanisms that underlie locomotor performance to explain the evolutionary patterns and ecological success of animals. Our aim was to determine the extent to which calcium (Ca2+) handling dynamics modulate the contractile properties of isolated skeletal muscle, and whether the effects of changing Ca2+ handling dynamics in skeletal muscle are paralleled by changes in whole-animal sprint and sustained swimming performance. Carp (Cyprinus carpio) increased swimming speed by concomitant increases in tail-beat amplitude and frequency. Reducing Ca2+ release from the sarcoplasmic reticulum (SR) by blocking ryanodine receptors with dantrolene decreased isolated peak muscle force and was paralleled by a decrease in tail-beat frequency and whole-animal sprint performance. An increase in fatigue resistance following dantrolene treatment may reflect the reduced depletion of Ca2+ stores in the SR associated with lower ryanodine receptor (RyR) activity. Blocking RyRs may be detrimental by reducing force production and beneficial by reducing SR Ca2+ depletion so that there was no net effect on critical sustained swimming speed (Ucrit). In isolated muscle, there was no negative effect on force production of blocking Ca2+ release via dihydropyridine receptors (DHPRs) with nifedipine. Nifedipine decreased fatigue resistance of isolated muscle, which was paralleled by decreases in tail-beat frequency and Ucrit. However, sprint performance also decreased with DHPR inhibition, which may indicate a role in muscle contraction of the Ca2+ released by DHPR into the myocyte. Inhibiting sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) activity with thapsigargin decreased fatigue resistance, suggesting that SERCA activity is important in avoiding Ca2+ store depletion and fatigue. We have shown that different molecular mechanisms modulate the same muscle and whole-animal traits, which provides an explanatory model for the observed variations in locomotor performance within and between species.
Effect of MT1 melatonin receptor deletion on melatonin‐mediated phase shift of circadian rhythms in the C57BL/6 mouse

Abstract: In the mouse suprachiasmatic nucleus (SCN), melatonin activates MT1 and MT2 G‐protein coupled receptors, which are involved primarily in inhibition of neuronal firing and phase shift of circadian rhythms. This study investigated the ability of melatonin to phase shift circadian rhythms in wild type (WT) and MT1 melatonin receptor knockout (KO) C57BL/6 mice. In WT mice, melatonin (90 μg/mouse, s.c.) administered at circadian time 10 (CT10; CT12 onset of activity) significantly phase advanced the onset of the circadian activity rhythm (0.60 ± 0.09 hr, n = 41) when compared with vehicle treated controls (−0.02 ± 0.07 hr, n = 28) (P < 0.001). In contrast, C57 MT1KO mice treated with melatonin did not phase shift circadian activity rhythms (−0.10 ± 0.12 hr, n = 42) when compared with vehicle treated mice (−0.12 ± 0.07 hr, n = 43). Similarly, in the C57 MT1KO mouse melatonin did not accelerate re‐entrainment to a new dark onset after an abrupt advance of the dark cycle. In contrast, melatonin (3 and 10 pm) significantly phase advanced circadian rhythm of neuronal firing in SCN brain slices independent of genotype with an identical maximal shift at 10 pm (C57 WT: 3.61 ± 0.38 hr, n = 3; C57 MT1KO: 3.45 ± 0.11 hr, n = 4). Taken together, these results suggest that melatonin‐mediated phase advances of circadian rhythms of neuronal firing in the SCN in vitro may involve activation of the MT2 receptor while in vivo activation of the MT1 and possibly the MT2 receptor may be necessary for the expression of melatonin‐mediated phase shifts of overt circadian activity rhythms.
Role of olivocerebellar system in timing without awareness

The timing of events can be implicit or without awareness yet critical for task performance. However, the neural correlates of implicit timing are unknown. One system that has long been implicated in event timing is the olivocerebellar system, which originates exclusively from the inferior olive. By using event-related functional MRI in human subjects and a specially designed behavioral task, we examined the effect of the subjects’ awareness of changes in stimulus timing on the olivocerebellar system response. Subjects were scanned while observing changes in stimulus timing that were presented near each subject's detection threshold such that subjects were aware of such changes in only approximately half the trials. The inferior olive and multiple areas within the cerebellar cortex showed a robust response to time changes regardless of whether the subjects were aware of these changes. Our findings provide support to the proposed role of the olivocerebellar system in encoding temporal information and further suggest that this system can operate independently of awareness and mediate implicit timing in a multitude of perceptual and motor operations, including classical conditioning and implicit learning.
Medical and Psychosexual Outcome in Women Affected by Complete Gonadal Dysgenesis

BACKGROUND Prenatal exposure to testicular hormones influences the development of brain structures and behavior in many non-human mammalian species. Less understood is the role of possessing a Y chromosome, independent of testicular hormones, on psychosexual differentiation. HYPOTHESIS Phenotypic women affected by complete gonadal dysgenesis possess a 46,XY chromosome complement and streak gonads. This population is suitable to test the influence of an absence of androgens and Müllerian inhibiting substance on psychosexual development in genetic males. PATIENTS Three 46,XY women diagnosed with complete gonadal dysgenesis participated. METHODS Psychosexual development, medical outcome and knowledge of medical condition were assessed with a written questionnaire and a physical examination. RESULTS All participants were healthy, compliant with their hormone therapy, and exhibited female-typical psychosexual development. However, participants were poorly informed about their condition and the fertility treatment options available to them. CONCLUSIONS These data indicate no obvious role for genes on the Y chromosome, outside of its pseudoautosomal region and SRY, on psychosexual differentiation in genetic males who do not produce testicular hormones. Greater efforts need to be made to educate affected women about their pregnancy options.
Using an interview study of transport use by people who have had a stroke to inform rehabilitation

Introduction: Many people who have had a stroke want to get out of their house more often. This study explored attitudes and barriers to the use of transport with the aim of informing rehabilitation. Method: Semi-structured interview study of 24 community-dwelling people who had had a stroke, purposively sampled to find people with a variety of recent experiences of transport. Interviews were taped and transcribed. Analysis was by constant comparative methodology, to develop emerging themes and concepts. Results: Interviewees wanted to travel for specific purposes but also for its own sake. Many could no longer use their car. This gave them less flexibility to travel and reduced their autonomy. Barriers to using alternative forms of transport were fear of injury or embarrassment from falling, an associated lack of confidence, inadequate information about transport services, perceptions about the cost of taxis and pavement vehicles (scooters) and environmental factors such as the weather. Those who could drive, or who lived with someone who did, gave the most positive descriptions of transport use. Those reliant on family or friends felt they could ask only for help getting to health-related appointments and those who used specialist transport services provided the most negative descriptions of transport. Conclusions: Many of the barriers to transport use after stroke may be amenable to intervention. An intervention package capable of re-enabling people to drive or be driven, to use a pavement scooter safely, to provide information about the alternatives and to encourage best use of public transport is worth developing.
Letrozole is more effective neoadjuvant endocrine therapy than tamoxifen for ErbB-1- and/or ErbB-2-positive, estrogen receptor-positive primary breast cancer: evidence from a phase III randomized trial.

PURPOSE Expression of ErbB-1 and ErbB-2 (epidermal growth factor receptor and HER2/neu) in breast cancer may cause tamoxifen resistance, but not all studies concur. Additionally, the relationship between ErbB-1 and ErbB-2 expression and response to selective aromatase inhibitors is unknown. A neoadjuvant study for primary breast cancer that randomized treatment between letrozole and tamoxifen provided a context within which these issues could be addressed prospectively. PATIENTS AND METHODS Postmenopausal patients with estrogen- and/or progesterone receptor-positive (ER+ and/or PgR+) primary breast cancer ineligible for breast-conserving surgery were randomly assigned to 4 months of neoadjuvant letrozole 2.5 mg daily or tamoxifen 20 mg daily in a double-blinded study. Immunohistochemistry (IHC) for ER and PgR was conducted on pretreatment biopsies and assessed by the Allred score. ErbB-1 and ErbB-2 IHC were assessed by intensity and completeness of membranous staining according to published criteria. RESULTS For study biopsy-confirmed ER+ and/or PgR+ cases that received letrozole, 60% responded and 48% underwent successful breast-conserving surgery. The response to tamoxifen was inferior (41%, P =.004), and fewer patients underwent breast conservation (36%, P =.036). Differences in response rates between letrozole and tamoxifen were most marked for tumors that were positive for ErbB-1 and/or ErbB-2 and ER (88% v 21%, P =.0004). CONCLUSION ER+, ErbB-1+, and/or ErbB-2+ primary breast cancer responded well to letrozole, but responses to tamoxifen were infrequent. This suggests that ErbB-1 and ErbB-2 signaling through ER is ligand-dependent and that the growth-promoting effects of these receptor tyrosine kinases on ER+ breast cancer can be inhibited by potent estrogen deprivation therapy.
Artificial neural networks as supervised techniques for FT‐IR microspectroscopic imaging

In this report the applicability of an improved method of image segmentation of infrared microspectroscopic data from histological specimens is demonstrated. Fourier transform infrared (FT‐IR) microspectroscopy was used to record hyperspectral data sets from human colorectal adenocarcinomas and to build up a database of spatially resolved tissue spectra. This database of colon microspectra comprised 4120 high‐quality FT‐IR point spectra from 28 patient samples and 12 different histological structures. The spectral information contained in the database was employed to teach and validate multilayer perceptron artificial neural network (MLP‐ANN) models. These classification models were then employed for database analysis and utilised to produce false colour images from complete tissue maps of FT‐IR microspectra. An important aspect of this study was also to demonstrate how the diagnostic sensitivity and specificity can be specifically optimised. An example is given which shows that changes of the number of teaching patterns per class can be used to modify these two interrelated test parameters. The definition of ANN topology turned out to be crucial to achieve a high degree of correspondence between the gold standard of histopathology and IR spectroscopy. Particularly, a hierarchical scheme of ANN classification proved to be superior for the reliable classification of tissue spectra. It was found that unsupervised methods of clustering, specifically agglomerative hierarchical clustering (AHC), were helpful in the initial phases of model generation. Optimal classification results could be achieved if the class definitions for the ANNs were carried out by considering the classification information provided by cluster analysis. Copyright © 2007 John Wiley & Sons, Ltd.
Testing the Assumptions of Slippery Slope Framework on Tax Compliance: Evidence from Nigerian SMEs

This paper integrates the assumptions of the Slippery Slope Framework in explaining the tax compliance of small and medium enterprises. This study tests these assumptions in relation to SMEs taxpayers to further reestablish the applicability of the framework with different kinds of taxpayers. Partial Least Squares Structural Equation Modeling (PLS - SEM) further supported the framework in analyzing the data of this study. The findings reveal that perceived corruption along with perceived service orientation strongly interact each other in expounding the paradox surrounding tax compliance.
Consumer Bankruptcy and Mortgage Default

We specify and estimate a rich model of consumption, housing demand and labor supply in an environment where individuals may file for bankruptcy or default on their mortgage. Uncertainty in the model is driven both by house price shocks and income shocks, while bankruptcy is governed by the basic institutional framework in the US as implied by chpater 7 and chapter 13. The model is estimated using micro data on credit reports and mortgages combined with individual level data from the American Community Survey. We perform several counterfactual experiments with the model which investigate welfare aspects of an important reform of the US consumer bankruptcy code implemented in 2006.
The Effects of Marijuana Liberalizations: Evidence from Monitoring the Future

By the end of 2016, 28 states had liberalized their marijuana laws: by decriminalizing possession, by legalizing for medical purposes, or by legalizing more broadly. More states are considering such policy changes even while supporters and opponents continue to debate their impacts. Yet evidence on these liberalizations remains scarce, in part due to data limitations.We use data from Monitoring the Future’s annual surveys of high school seniors to evaluate the impact of marijuana liberalizations on marijuana use, other substance use, alcohol consumption, attitudes surrounding substance use, youth health outcomes, crime rates, and traffic accidents. These data have several advantages over those used in prior analyses. We find that marijuana liberalizations have had minimal impact on the examined outcomes. Notably, many of the outcomes predicted by critics of liberalizations, such as increases in youth drug use and youth criminal behavior, have failed to materialize in the wake of marijuana liberalizations.
What do we know about the dividend puzzle? – A literature survey

Purpose The purpose of this paper is to shed light on the ongoing debate of dividend policy, which is considered one of the most controversial topics in corporate finance literature. Design/methodology/approach The paper provides a survey of literature; it, first, outlines the main theoretical arguments of dividend policy and then critically discusses the most important and influential previous empirical studies in the dividend literature. Findings The analysis of literature review detects that no general consensus has yet been reached after many decades of investigation, despite extensive debate and countless research. Consequently, the main motivation for paying dividends is still unsolved and thus remains as a puzzle. In addition, there is no doubt that carrying the dividend debate into the context of emerging markets attaches more pieces to this puzzle. Originality/value This paper offers an updated and more comprehensive survey of literature by examining the relationship between theory and practice from both developed and emerging markets.
Global sport, nationalism and national identity construction: the case of naturalised Chinese table tennis players in South Korea

The purpose of this research is to analyse the process of South Korean nationalism and national identity construction through studying Chinese table tennis players that have become naturalised citizens of South Korea. Based on an Eliasian theoretical and methodological prospective, this research employs three different methods of analysis. First, it employs archival analysis in the historical context chapter to determine the origins of table tennis and its spread to Northeast Asia, the political and societal role of table tennis in China and South Korea, and the appearance of the first naturalised player in South Korea. Second, this study employs newspaper text analysis to investigate newspaper reports of the South Korean and Chinese table tennis matches from the 1988 Seoul Olympics Games to the 2012 Games in London. This section first examines the flow of Korean nationalism through the table tennis matches between Korea and China, and then explores how the newspaper reports expressed nationalism in regard to the Korean players and naturalised players. Third, this study employs an interview method to gather data from 17 interviewees who have been associated directly or indirectly associated with the Chinese naturalised table tennis players in order to further examine the process of the formation of South Korean national identity. The first analysis regarding historical findings identifies that table tennis has evolved precisely alongside Elias s sportisation theory. In South Korea and China, table tennis has played the role of a national sport. Especially in South Korea, table tennis was a nationalistic sport that opposed anti-colonialism and anti-communism. It was in table tennis that the first naturalised athlete in the history of South Korean sport was selected for the Olympics. In the second analysis, through examining news reports of the Olympic table tennis matches between China and South Korea, this research identifies that South Korean newspapers continue to generate a strong image of nationalism. Moreover, the newspaper reports do not use the same nationalistic sentiments and personal pronouns for South Korean players and naturalised players. Finally, the interview analysis investigates the formation of South Korean identity through the naturalised table tennis players in South Korea. Three national identities groups have arisen in South Korea in response to the emergence of naturalised athletes. The first group approves of globalisation and naturalisation. The second group opposes globalisation and naturalisation. The third group, which comprises the media, has no specific interest in globalisation and naturalisation, but has an identity that changes based on the interests of the media company. In other words, the evidence from the interviews carried out for this thesis shows that South Korean national identity in regard to naturalised athletes remains diversified and not unified.
Prevalence and Predictive Factors of Chronic Postsurgical Pain and Poor Global Recovery 1 Year After Outpatient Surgery

Objectives:To prospectively describe the prevalence and predictive factors of chronic postsurgical pain (CPSP) and poor global recovery in a large outpatient population at a university hospital, 1 year after outpatient surgery. Materials and Methods:A prospective longitudinal cohort study was performed. During 18 months, patients presenting for preoperative assessment were invited to participate. Outcome parameters were measured by using questionnaires at 3 timepoints: 1 week preoperatively, 4 days postoperatively, and 1 year postoperatively. A value of >3 on an 11-point numeric rating scale was considered to indicate moderate to severe pain. A score of ⩽80% on the Global Surgical Recovery Index was defined as poor global recovery. Results:A total of 908 patients were included. The prevalence of moderate to severe preoperative pain was 37.7%, acute postsurgical pain 26.7%, and CPSP 15.3%. Risk factors for the development of CPSP were surgical specialty, preoperative pain, preoperative analgesic use, acute postoperative pain, surgical fear, lack of optimism, and poor preoperative quality of life. The prevalence of poor global recovery was 22.3%. Risk factors for poor global recovery were recurrent surgery because of the same pathology, preoperative pain, preoperative analgesic use, surgical fear, lack of optimism, poor preoperative and acute postoperative quality of life, and follow-up surgery during the first postoperative year. Discussion:Moderate to severe CPSP after outpatient surgery is common, and should not be underestimated. Patients at risk for developing CPSP can be identified during the preoperative phase.
Benefits of PEGylation in the early post-transplant period of intraportal islet transplantation as assessed by magnetic resonance imaging of labeled islets

While a few studies have demonstrated the benefit of PEGylation in islet transplantation, most have employed renal subcapsular models and none have performed direct comparisons of islet mass in intraportal islet transplantation using islet magnetic resonance imaging (MRI). In this study, our aim was to demonstrate the benefit of PEGylation in the early post-transplant period of intraportal islet transplantation with a novel algorithm for islet MRI. Islets were PEGylated after ferucarbotran labeling in a rat syngeneic intraportal islet transplantation model followed by comparisons of post-transplant glycemic levels in recipient rats infused with PEGylated (n = 12) and non-PEGylated (n = 13) islets. The total area of hypointense spots and the number of hypointense spots larger than 1.758 mm2 of PEGylated and non-PEGylated islets were quantitatively compared. The total area of hypointense spots (P < 0.05) and the number of hypointense spots larger than 1.758 mm2 (P < 0.05) were higher in the PEGylated islet group 7 and 14 days post translation (DPT). These results translated into better post-transplant outcomes in the PEGylated islet group 28 DPT. In validation experiments, MRI parameters obtained 1, 7, and 14 DPT predicted normoglycemia 4 wk post-transplantation. We directly demonstrated the benefit of islet PEGylation in protection against nonspecific islet destruction in the early post-transplant period of intraportal islet transplantation using a novel algorithm for islet MRI. This novel algorithm could serve as a useful tool to demonstrate such benefit in future clinical trials of islet transplantation using PEGylated islets.
Nonrandom Chromosomal Numerical Abnormality Predicting Prognosis of Gastric Cancer: A Retrospective Study of 51 Cases Using Pathology Archives

Chromosomal or centromerical numerical abnormality (CNA) is a well-known characteristic of human cancer, but the extensive and specific documentation of CNA in gastric cancer is still sparse, partly because of difficulty in obtaining cytogenetic information. Taking advantage of a recently developed fluorescence in situ hybridization protocol for formalin-fixed paraffin-embedded tissues, we investigated CNA of 51 gastric cancer cases with a panel of 18 chromosome-specific α-satellite probes (for chromosomes 1–4, 6–12, 15-18, 20, X and Y) and region specific probes (c-myc and p53) to enumerate respective chromosome numbers in interphase nuclei. The involved chromosomes exhibiting CNA were nonrandom in gastric cancer. Aberrations of chromosomes 1, 8, 17, 20, and X were frequent regardless of histologic types, whereas aberrations chromosomes 10, 15, and 18 occurred less often (p < 0.001). From a histopathologic standpoint, the mucocellular type had stable CNA in comparison with the tubular type (mucocellular type vs tubular type carcinoma: 21.0 ± 10.63% vs 62.8 ± 12.79%, p < 0.001). Interestingly, there was less extensive CNA in women (men vs women: 54.3 ± 9.49% versus 24.9 ± 12.23%, p < 0.001). A dramatic difference in the outcome was detected according to the involvement of chromosomes 3, 10, 11, 12, 17, and Y; that is, the cases with CNA of these chromosomes had worse prognosis.