Miracle-dz/UniFAR-FTUs huggingface dataset:
Predicting Long-Term Outcome after Endovascular Stroke Treatment: The Totaled Health Risks in Vascular Events Score

BACKGROUND AND PURPOSE: Endovascular treatments are being increasingly used in acute ischemic stroke, and better tools are needed to determine which patients may benefit most from these techniques. We hypothesized that specific chronic diseases can be used, along with age and stroke severity, to predict endovascular stroke treatment outcomes. MATERIALS AND METHODS: Data from 2 single-arm trials of a thrombectomy device, MERCI and Multi MERCI, were pooled for analysis. A predictive score was developed by using the independent contribution of variables in multivariable analysis. RESULTS: HTN, DM, and AFib were found to predict outcomes. These 3 conditions contribute equally to a CDS that predicts outcomes independent of other predictor variables, including age, stroke severity, and vessel recanalization. A 10-level predictive score, the THRIVE score, which incorporates age, stroke severity, and the CDS, was developed. The THRIVE score strongly predicts outcome and mortality at 90 days. CONCLUSIONS: Specific chronic diseases influence poststroke outcomes among patients undergoing endovascular stroke treatment, independent of other predictors of outcome. The THRIVE score reflects the contributions of chronic disease, age, and stroke severity and strongly predicts endovascular stroke treatment outcomes.
Chronic fatigue syndrome (cfs)

clea: of people are suffering from une>cplained prolonged fatigue, Iow grade feve; headache, myalgia, arthralgia, a sense of exhaustion, cognitive clysfunction, depression, and sleep disturbance because of Most of patients with are housebound for a long time. and now responding we proposed the new hypothesis of the pathogenesis of understood a spe-cial condition based on the abnormality of the psycho-neuro-endocrino-immunological systern caused by the psycho-social stress and sorne genetic components. conditions, a reactivation of various kinds of herpes virus might occur as a result of immune causing of several cytokines. of secondary the abnormal production of such cytokines. of neuropsychological symptoms. In this papeg we describe not only the pathophysiology of the CFS, but also an overview of the neuro-molecular mechanisms
Chemical characterization of agroforestry solid residues aiming its utilization as adsorbents for metals in water

In this work, a study of the correlation between the functional groups present in the chemical structure of the fibers of coconut shells, cocoa and eucalyptus, and their adsorption capacity of Cd+2 and Cu+2 ions from water was performed. The content of soluble solids and reactive phenols in aqueous extracts were determined. The chemical functional groups present in the fibers were examined using the IR spectra. The adsorption capacity of the peels was determined using atomic absorption spectrophotometer. For Cd+2, a significant correlation between the adsorption capacity and some specific chemical functional groups present in the fiber was verified. The potential use of these peels, as adsorbent of Cd+2 ions, is based on the presence of OH functional groups such as aryl-OH, aryl-O-CH2 of phenol carboxylic acids, as well as carbonyl groups derived from carboxylic acid salts, in these fibers.
Diffusion-weighted MRI compared to FDG PET/CT for assessment of early treatment response in lymphoma

Background 18F fluoro-deoxyglucose (FDG) positron emission tomography / computed tomography (PET/CT) is a well-recognized diagnostic tool used for staging and monitoring of therapy response for lymphomas. During the past decade diffusion-weighted (DW) magnetic resonance imaging (MRI) is increasingly being included in the assessment of tumor response for various cancers. Purpose To compare the change in maximum standardized uptake value (ΔSUVmax) from FDG PET/CT with the change in apparent diffusion coefficient (ΔADC) from DW MRI after initiation of the first cycle of chemotherapy in patients with Hodgkin's lymphoma (HL) and in patients with diffuse large B-cell lymphoma (DLBCL). Material and Methods Twenty-seven consecutive patients with histologically proven lymphoma and lymphomatous lymph nodes (LLN) of the neck (19 with HL, 8 with DLBCL) underwent FDG PET/CT and MRI of the neck before and after initiation of the first cycle of chemotherapy. The mean time interval from initiation of chemotherapy to imaging was 19 days and 2 days for FDG PET/CT and MRI, respectively. For each patient ΔSUVmax, ΔADC, and change in volume of the same LLN were compared. Results There was a significant mean decrease of SUVmax by 70%, but no significant change in ADC. There was no significant reduction in LLN volume. Conclusion There was no significant correlation between ΔSUVmax and ΔADC. Thus, our data do not support that FDG PET/CT can be replaced by early DW MRI for response evaluation in lymphoma patients.
Acetylation of p53 Inhibits Its Ubiquitination by Mdm2*

In response to DNA damage, the activity of the p53 tumor suppressor is modulated by protein stabilization and post-translational modifications including acetylation. Interestingly, both acetylation and ubiquitination can modify the same lysine residues at the C terminus of p53, implicating a role of acetylation in the regulation of p53 stability. However, the direct effect of acetylation on Mdm2-mediated ubiquitination of p53 is still lacking because of technical difficulties. Here, we have developed a method to obtain pure acetylated p53 proteins from cells, and by using an in vitro purified system, we provide the direct evidence that acetylation of the C-terminal domain is sufficient to abrogate its ubiquitination by Mdm2. Importantly, even in the absence of DNA damage, acetylation of the p53 protein is capable of reducing the ubiquitination levels and extending its half-life in vivo. Moreover, we also show that acetylation of p53 can affect its ubiquitination through other mechanisms in addition to the site competition. This study has significant implications regarding a general mechanism by which protein acetylation modulates ubiquitination-dependent proteasome proteolysis.
Interaction of Age With Shift-Related Sleep-Wakefulness, Sleepiness, Performance, and Social Life

It is not clear how the age-related changes in sleep are related to performance and subjective sleepiness at different time of the day. The aim of the present study was to study work shift related interactions of age with sleep-wakefulness, performance, and social life. A representative sample of aircraft maintenance workers in a continuous three-shift system was studied by a questionnaire (n = 275) and an on-site field (n = 49) study. In the field study, sleep length and quality and different ratings of social and other activities were studied with an actigraphy and a Pocket PC diary during 15 consecutive days. Subjective sleepiness (KSS) and vigilance performance (PVT) were registered at work. Although the shift type influenced the sleep, subjective sleepiness, performance, and social life, age was distinctly related only to shift-related changes in the amount of sleep, subjective sleepiness, and psychomotor vigilance. Night shifts were related with shorter sleep, decreased performance, and increased sleepiness. Although subjective sleepiness was greatest among the youngest (25–34 years) age group during the morning and the night shifts, the increase of performance lapses was higher among the middle-aged (35–49 years) and senior (50–58 years) groups during the night shifts compared to the youngest age group. According to the questionnaire, older shiftworkers also tended to perceive more frequently that subjective sleepiness decreases their work performance during the morning and night shifts. The results indicate of no direct link between age-related differences in subjective sleepiness and performance at night work. The shorter day sleep after the night shifts and higher deterioration of subjective and objective performance according to age urge on development of shift schedules aiming at lower fatigue levels during the night shifts.
Regulation of epiblast cell movements by chondroitin sulfate during gastrulation in the chick

Avian gastrulation is dependent on the ingression of outer layer cells into the interior of the embryo by means of a transient structure referred to as the primitive streak. As the growing streak progresses through the central area pellucida of the blastoderm, selective de‐epithelialization of epiblast cells results in the initial migratory cells of the primitive mesoderm and endoderm. Here, we have examined the possibility that extracellular matrix molecules of the epiblast basal lamina influence the selection of streak‐specific epiblast cells. By using whole embryo culture, we have found that removal of chondroitin sulfate glycosaminoglycans at gastrulation stages leads to defective streak formation. In situ hybridization with streak‐specific markers in these embryos reveals ectopic patterns of gene expression, suggesting that differentiation of primitive streak precursors in the pregastrula epiblast is independent of normal streak morphogenesis. In addition, in vitro assays with chondroitin sulfate containing matrices suggest that specific cells of the epiblast are inhibited from joining the streak during gastrulation. Taken together, these results indicate that the presence of chondroitin sulfate in the epiblast basal lamina facilitates the allocation of cells to the primary germ layers by preventing ectopic axis formation. © 2000 Wiley‐Liss, Inc.
A Task‐Based Ontology Approach to Automate Geospatial Data Retrieval

This paper presents a task‐based and Semantic Web approach to find geospatial data. The purpose of the project is to improve data discovery and facilitate automatic retrieval of data sources. The work presented here helps create the beginnings of a Geospatial Semantic Web. The intent is to create a system that provides appropriate results to application users who search for data when facing tasks such as emergency response or planning activities. In our task‐based system, we formalize the relationships between types of tasks, including emergency response, and types of data sources needed for those tasks. Domain knowledge, including criteria describing data sources, is recorded in an ontology language. With the ontology, reasoning can be done to infer various types of information including which data sources meet specific criteria for use in particular tasks. The vision presented here is that in an emergency, for example, a user accesses a Web‐based application and selects the type of emergency and the geographic area. The application then returns the types and locations (URLs) of the specific geospatial data needed. We explore the abilities and limitations of the OWL Web Ontology Language along with other Semantic Web technologies for this purpose.
Estimation of child's biological age based on tooth development

Dental development is relatively independent from other systems maturation. Recent studies have reported changes in the timing of tooth development for contemporary children comparing to children that lived more than 30 years ago (secular trend). Detectable variations in the tempo of tooth mineralization and duration of tooth maturation between children from different geographical regions were reported. Dental age is important not only for dental specialists, but also for pediatricians (in the evaluation of growth and development of healthy children, in pediatric endocrinology, in children with different diseases or syndromes) and forensic doctors (in order to estimate the age or to identify the child). The aim of our study was to investigate the regional characteristics of dental maturation in actual Romanian children. We conducted a cross-sectional study on a final sample of 441 radiographs of patients aged between 5.5 and 14.5 years (218 girls and 223 boys). The dental panoramic radiographs were scored by two examiners and intra- and inter- examiner calibration was made. We used a dedicated software for easy scoring, automatic dental age determination and as a database. On average, the Romanian girls showed an overestimation of 0.36 years, meaning 132 days, p=0.129, α = 0.05 and boys an underestimation of 0.04 years, meaning 15 days, p = 0.852, α = 0.05. New tables were developed in order to convert dental maturity calculated according to Demirjian method into dental age of contemporary Romanian children.
Regulation of human DNA polymerase delta in the cellular responses to DNA damage

The p12 subunit of polymerase delta (Pol δ) is degraded in response to DNA damage induced by UV, alkylating agents, oxidative, and replication stresses. This leads to the conversion of the Pol δ4 holoenzyme to the heterotrimer, Pol δ3. We review studies that establish that Pol δ3 formation is an event that could have a major impact on cellular processes in genomic surveillance, DNA replication, and DNA repair. p12 degradation is dependent on the apical ataxia telangiectasia and Rad3 related (ATR) kinase and is mediated by the ubiquitin–proteasome system. Pol δ3 exhibits properties of an “antimutator” polymerase, suggesting that it could contribute to an increased surveillance against mutagenesis, for example, when Pol δ carries out bypass synthesis past small base lesions that engage in spurious base pairing. Chromatin immunoprecipitation analysis and examination of the spatiotemporal recruitment of Pol δ to sites of DNA damage show that Pol δ3 is the primary form of Pol δ associated with cyclobutane pyrimidine dimer lesions and therefore should be considered as the operative form of Pol δ engaged in DNA repair. We propose a model for the switching of Pol δ with translesion polymerases, incorporating the salient features of the recently determined structure of monoubiquitinated proliferating cell nuclear antigen and emphasizing the role of Pol δ3. Because of the critical role of Pol δ activity in DNA replication and repair, the formation of Pol δ3 in response to DNA damage opens the prospect that pleiotropic effects may ensue. This opens the horizons for future exploration of how this novel response to DNA damage contributes to genomic stability. Mol. Mutagen. 2012. © 2012 Wiley Periodicals, Inc.
Effect of Warm-Ups Involving Static or Dynamic Stretching on Agility, Sprinting, and Jumping Performance in Trained Individuals

Chaouachi A, Castagna C, Chtara M, Brughelli M, Turki O, Galy O, Chamari K, and Behm DG. Effect of warm-ups involving static or dynamic stretching on agility, sprinting, and jumping performance in trained individuals. J Strength Cond Res 24(8): 2001-2011, 2010-The objective of the present study was to investigate the effects of static and dynamic stretching alone and in combination on subsequent agility, sprinting, and jump performance. Eight different stretching protocols: (a) static stretch (SS) to point of discomfort (POD); (b) SS less than POD (SS<POD); (c) dynamic stretching (DS); (d) SS POD combined with DS (SS POD + DS); (v) SS<POD combined with DS (SS<POD + DS); (vi) DS combined with SS POD (DS + SS POD); (vii) DS combined with SS<POD (DS + SS<POD); and (viii) a control warm-up condition without stretching were implemented with a prior aerobic warm-up and followed by dynamic activities. Dependent variables included a 30-m sprint, agility run, and jump tests. The control condition (4.2 ± 0.15 seconds) showed significant differences (p = 0.05) for faster times than the DS + SS<POD (4.28s ± 0.17) condition in the 30-m (1.9%) sprint. There were no other significant differences. The lack of stretch-induced impairments may be attributed to the trained state of the participants or the amount of time used after stretching before the performance. Participants were either professional or national level elite athletes who trained 6-8 times a week with each session lasting ∼90 minutes. Based on these findings and the literature, trained individuals who wish to implement static stretching should include an adequate warm-up and dynamic sport-specific activities with at least 5 or more minutes of recovery before their sport activity.
PROBING DYNAMICAL PROCESSES IN THE PLANET-FORMING REGION WITH DUST MINERALOGY

We present Herschel Space Observatory PACS spectra of GQ Lup, a protoplanetary disk in the Lupus star-forming region. Through spectral energy distribution fitting from 0.3 μm to 1.3 mm, we construct a self-consistent model of this system's temperature and density structures, finding that although it is 3 Myr old, its dust has not settled to the midplane substantially. The disk has a radial gradient in both the silicate dust composition and grain size, with large amorphous grains in the upper layers of the inner disk and an enhancement of submicron, crystalline grains in the outer disk. We detect an excess of emission in the Herschel PACS B2A band near 63 μm and model it with a combination of ∼15–70 μm crystalline water ice grains with a size distribution consistent with ice recondensation-enhanced grain growth and a mass fraction half of that of our solar system. The combination of crystalline water ice and silicates in the outer disk is suggestive of disk-wide heating events or planetesimal collisions. If confirmed, this would be the first detection of water ice by Herschel.
Entropy Analysis in a Bubble Column Based on Ultrafast X-Ray Tomography Data

By means of the ultrafast X-ray tomography facility, data were obtained at different superficial gas velocities UG in a bubble column (0.1 m in ID) operated with an air-deionized water system at ambient conditions. Raw reconstructed images were treated by both the information entropy (IE) and the reconstruction entropy (RE) algorithms in order to identify the main transition velocities in a bubble column. The IE values exhibited two well-pronounced minima at UG=0.025 m/s and UG=0.085 m/s identifying the boundaries of the homogeneous, transition and heterogeneous regimes. The RE extracted from the central region of the column’s cross-section exhibited only one characteristic peak at UG=0.03 m/s, which was attributed to the transition from the homogeneous to the heterogeneous flow regime. This result implies that the transition regime is non-existent in the core of the column. Keywords—Bubble column, ultrafast X-ray tomography, information entropy, reconstruction entropy.
Ascorbic Acid in Mesencephalic Cultures: Effects on Dopaminergic Neuron Development

Abstract: Ascorbic acid exists in high intracellular concentrations in fetal rat brain. In mesencephalic cultures the cellular ascorbic acid content drops sharply to undetectable levels when no ascorbic acid is added to the medium, thus creating a model of scorbutic neuronal tissue and affording the study of ascorbic acid's effects on mesencephalic cell development and function. Cultures treated with 0.2 mM ascorbic acid were compared with controls (scorbutic cultures) by using morphological and biochemical indices. Ascorbic acid cultures at 7 and 14 days in vitro showed a marked increase in glial proliferation on glial fibrillary acidic protein staining and increased neurite growth and number on tyrosine hydroxylase staining. Significantly higher dopamine uptake and levels of dopamine and 3, 4‐dihydroxyphenylacetic acid were also observed after 7 and 14 days of ascorbic acid treatment. The capacity to accumulate ascorbic acid and the ability to retain the intracellular ascorbic acid developed gradually as the cultures matured. Ascorbic acid reached the embryonal levels by day 14 in vitro. We conclude that although neuronal cultures can survive and grow in the absence of detectable levels of ascorbic acid, its presence exerts a broad effect on dopamine neuron morphology and biochemical functioning either directly or through increased glial proliferation, or possibly both.
Dynamics of T‐cell infiltration during the course of ovarian cancer: The gradual shift from a Th17 effector cell response to a predominant infiltration by regulatory T‐cells

The type of immune cells that are present within the tumor microenvironment can play a crucial role in the survival of patients. However, little is known about the dynamics of the tumor‐infiltrating immune cells during disease progression. We studied the immune cells that infiltrated the tumor tissues of ovarian cancer patients at different stages of disease. The early stages of development of ovarian carcinomas were characterized by a strong Th17 immune response, whereas in stage II patients, recruitment of high numbers of Th1 cells was observed. In disseminated tumors (Stages III–IV), we detected a dominant population of Helios+ activated regulatory T cells (Tregs) along with high numbers of monocytes/macrophages and myeloid dendritic cells (mDCs). Tumor‐infiltrating Tregs had markedly lower expression of CCR4 than circulating Tregs, and the numbers of tumor‐infiltrating Tregs significantly correlated with the levels of CCL22 in ovarian tumor cell culture supernatants, suggesting their recruitment via a CCR4/CCL22 interaction. CCL22 was mainly produced by tumor cells, monocytes/macrophages and mDCs in the primary ovarian tumors, and its expression markedly increased in response to IFNγ. Taken together, the specific recruitment of Tregs, probably triggered by inflammatory stimuli, leads to a significant immune suppression in the advanced stages of ovarian cancer.
Proteomic analysis of β‐catenin activation in mouse liver by DIGE analysis identifies glucose metabolism as a new target of the Wnt pathway

The Wnt/β‐catenin signaling pathway has been increasingly implicated in liver development and physiology. Aberrant activation of this pathway is one of the major genetic events observed during the process of human HCC development. To gain insight into the mechanism underlying β‐catenin action in the liver, we conducted a quantitative differential proteomic analysis using 2‐D DIGE combined with MS, in mice with liver‐specific deletion of Apc resulting in acute activation of β‐catenin signaling (ApcKOliv mice). We identified 94 protein spots showing differential expression between mutant ApcKOliv and control mice, corresponding to 56 individual proteins. Most of the proteins identified were associated with metabolic pathways, such as ammonia and glucose metabolism. Our analysis showed an increase in lactate dehydrogenase activity together with a downregulation of two mitochondrial ATPase subunits (ATP5a1 and ATP5b). These observations indicate that β‐catenin signaling may induce a shift in the glucose metabolism from oxidative phosphorylation to glycolysis, known as the “Warburg effect”. Imaging with 18F‐fluoro‐2‐deoxy‐D‐glucose‐positron emission tomography suggests that the specific metabolic reprogramming induced by β‐catenin in the liver does not imply the first step of glycolysis. This observation may explain why some HCCs are difficult to assess by fluoro‐2‐deoxy‐D‐glucose‐positron emission tomography imaging.