Miracle-dz/UniFAR-FTUs huggingface dataset:
Oral findings in patients with obstructive sleep apnea syndrome.

OBJECTIVE To evaluate the role of dental parameters that may contribute in increasing apneic activity in patients with obstructive sleep apnea syndrome (OSAS). METHOD AND MATERIALS A total of 152 subjects (121 males and 31 females ) were examined from October 1999 until September 2001, for sleep disorder symptoms. All patients underwent polysomnography. Any abnormal or unusual features that could inhibit the upper airway of the oral cavity were evaluated by an oral pathologist. RESULTS Statistical analysis of the independent structural variables and the respiratory disturbance index proved to be significant only in the cases of retropositioned or narrow hard palate with a vertically positioned soft palate, the type of breathing (oral breathing), and the enlarged uvula. CONCLUSION The oral soft tissues seem to be more closely associated with OSAS. Therefore, the diagnosis of the structural features of the oral cavity by the clinician is useful to predict apneic activity.
Circadian genes in a blind subterranean mammal II: Conservation and uniqueness of the three Period homologs in the blind subterranean mole rat, Spalax ehrenbergi superspecies

We demonstrated that a subterranean, visually blind mammal has a functional set of three Per genes that are important components of the circadian clockwork in mammals. The mole rat superspecies Spalax ehrenbergi is a blind subterranean animal that lives its entire life underground in darkness. It has degenerated eyes, but the retina and highly hypertrophic harderian gland are involved in photoperiodic perception. All three Per genes oscillate with a periodicity of 24 h in the suprachiasmatic nuclei, eye, and harderian gland and are expressed in peripheral organs. This oscillation is maintained under constant conditions. The light inducibility of sPer1 and sPer2, which are similar in structure to those of other mammals, indicates the role of these genes in clock resetting. However, sPer3 is unique in mammals and has two truncated isoforms, and its expressional analysis leaves its function unresolved. Per's expression analysis in the harderian gland suggests an important participation of this organ in the stabilization and resetting mechanism of the central pacemaker in the suprachiasmatic nuclei and in unique adaptation to life underground.
Would older medical patients use psychological services?

PURPOSE This study examines older patient preferences for psychological services, including the types of services they would be interested in and who should provide them. DESIGN AND METHODS We surveyed 183 primary care patients aged 55 and older on their current level of psychiatric distress and preferences for psychological services. RESULTS Seventy-nine percent of this sample said they would use any of the psychological services we presented to them. Seventy-two percent preferred to talk to their primary care provider, and 46% of the sample indicated that they would also speak with a mental health worker or nurse about their problems. Few older people said they would attend group psychotherapy, but 69% said they would attend psychoeducational classes. IMPLICATIONS Our findings suggest that older adults would be amenable to psychosocial services, particularly individual services and psychoeducational programming. Policy makers interested in improving the quality of mental health treatment in primary care medicine should consider the inclusion of psychological services as a treatment option.
Sustained Education Access for Homeless Youth: Case Study of U.S. Transitional Living Programs

Sustained Education Access for Homeless Youth: Case Study of U.S. Transitional Living Programs by Heather P. Hall MSPP, Georgia Institute of Technology, 2004 MBA, Mercer University, 1999 BBA, Pace University, 1982 Dissertation Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy Public Policy and Administration Walden University February 2017 Abstract Without interventions to address homelessness among youth, the risk of homelessness for future generations is great. As a result, the federal government has invested in funding Transitional Living Programs (TLPs), though it is not clear whether these programs have achieved the intended outcomes of helping youth transition from homelessness to beingWithout interventions to address homelessness among youth, the risk of homelessness for future generations is great. As a result, the federal government has invested in funding Transitional Living Programs (TLPs), though it is not clear whether these programs have achieved the intended outcomes of helping youth transition from homelessness to being able to sustain employment and avoid poverty through access to educational and workforce programs. Using the broad conceptualization of democratic governance as the foundation, the purpose of this case study was to assess whether access to educational programs through TLPs benefitted homeless youth in terms of program success. Data for this study were collected through interviews with 9 administrators of TLP service providers and publicly available documents for the years 2008-2014. Interview data were inductively coded and subjected to thematic analysis, and data from public sources were evaluated using descriptive statistics. Qualitative analysis revealed that long term, postTLP outcomes were difficult to track as individual TLPs tended to lose contact with youth, though self-advocacy for participants, coupled with the ability to sustain long term independence, were keys to success for program participants. Participants also noted their perception that education through TLPs provide opportunity for stable social and economic connections. Positive social change resulting from this study may be attained if TLP long-term outcomes are evaluated using metrics that are realistic for the target population, and organizational goals are refocused on improving opportunities for youth to make meaningful contributions to their communities, and thereby build the social equity necessary for long-term success. Sustained Education Access for Homeless Youth: Case Study of U.S. Transitional Living Programs by
Direct Measurement of Antigen Binding Properties of CD1 Proteins Using Fluorescent Lipid Probes*

CD1 proteins are antigen-presenting molecules that bind foreign and self-lipids and stimulate specific T cell responses. In the current study, we investigated ligand binding by CD1 proteins by developing a fluorescent probe binding approach using soluble recombinant human CD1 proteins. To increase stability and yield, soluble group 1 CD1 (CD1b and CD1c) and group 2 CD1 (CD1d) proteins were produced as single chain secreted CD1 proteins in which β2-microglobulin was fused to the N termini of the CD1 heavy chains by a flexible peptide linker sequence. Analysis of ligand binding properties of single chain secreted CD1 proteins by using fluorescent lipid probes indicated significant differences in ligand preference and in pH dependence of binding by group 1 versus group 2 CD1 proteins. Whereas group 1 CD1 isoforms (CD1b and CD1c) show stronger binding of nitrobenzoxadiazole (NBD)-labeled dialkyl-based ligands (phosphatidylcholine, sphingomyelin, and ceramide), group 2 CD1 (CD1d) proteins were stronger binders of small hydrophobic probes such as 1-anilinonaphthalene-8-sulfonic acid and 4,4′-dianilino-1,1′-naphthyl-5,5′-disulfonic acid. Competition studies indicated that binding of fluorescent lipid probes involved association of the probe with the hydrophobic ligand binding groove of CD1 proteins. Analysis of selected alanine substitution mutants of human CD1b known to inhibit antigen presentation showed that NBD-labeled lipid probe binding could be used to distinguish mutations that interfere with ligand binding from those that affect T cell receptor docking. Our findings provide further evidence for the functional specialization of different CD1 isoforms and demonstrate the value of the fluorescent lipid probe binding method for assisting structure-based studies of CD1 function.
Indispensable Role of Ion Channels and Transporters in the Auditory System

Ear is a complex system where appropriate ionic composition is essential for maintaining the tissue homeostasis and hearing function. Ion transporters and channels present in the auditory system plays a crucial role in maintaining proper ionic composition in the ear. The extracellular fluid, called endolymph, found in the cochlea of the mammalian inner ear is particularly unique due to its electrochemical properties. At an endocochlear potential of about +80 mV, signaling initiated by acoustic stimuli at the level of the hair cells is dependent on the unusually high potassium (K+) concentration of endolymph. There are ion channels and transporters that exists in the ear to ensure that K+ is continually being cycled into the stria media endolymph. This review is focused on the discussion of the molecular and genetic basis of previously and newly recognized ion channels and transporters that support sensory hair cell excitation based on recent knock‐in and knock‐out studies of these channels. This article also addresses the molecular and genetic defects and the pathophysiology behind Meniere's disease as well as how the dysregulation of these ion transporters can result in severe defects in hearing or even deafness. Understanding the role of ion channels and transporters in the auditory system will facilitate in designing effective treatment modalities against ear disorders including Meniere's disease and hearing loss. J. Cell. Physiol. 232: 743–758, 2017. © 2016 Wiley Periodicals, Inc.
Self-Leadership Strategies & Performance Perspectives Within Student Aviation Teams

The use of teams to achieve organizational goals requires companies to employ individuals who are competent at both performing individual tasks and working well with others. This study examines the relationship between performance strategies and the performance perceived by teammates and supervisors. Previous research indicates a positive relationship between individual work role performance and performances strategies associated with self-leadership. Self-leadership can be conceptualized as a set of self-influence strategies used by individuals to increase personal effectiveness. These strategies include functions such as self-goal setting and positive self-talk. In this study, experts supervised and rated individual work role performance using instruments developed within the research setting, and peers rated one another using the comprehensive assessment of team member effectiveness (Ohland et al., 2012). Ratings from peers, supervisors, and self-reported self-leadership were compared with one another in a correlational design. Self-leadership was measured using the abbreviated self-leadership questionnaire (Houghton & Neck, 2012), the psychometric properties of which were also examined. Participants were aerospace students at a southern university engaged in operating a simulated flight dispatch center for course credit. A positive, statistically significant relationship was found between perceived team member effectiveness and expert-rated individual performance; however, the self-leadership strategies measured in this study were unrelated to the criterion variables. The examination of the self-leadership measure indicates that the construct was not adequately captured.
Heart rate variability during night sleep and after awakening in overtrained athletes.

PURPOSE This study was conducted to test the hypothesis of autonomic imbalance in overtrained athletes during sleep and after awakening with analyses of heart rate variability (HRV) and nocturnal urine stress hormones. METHODS We examined 12 athletes diagnosed to be severely overtrained (OA, 6 men and 6 women, mean age (+/-SD) 25 +/- 7 yr) and 12 control athletes (CA, 6 men and 6 women, mean age 24 +/- 5 yr). Overtraining diagnosis was further supported by higher perceived stress in OA than in CA (24.8 +/- 10.8 vs 15.3 +/- 5.5, P < 0.05). HRV was analyzed with time and frequency domain methods from RR intervals (RRI) recorded during sleep and after awakening. Nocturnal urine stress hormones were analyzed by liquid chromatography. RESULTS No differences were found in HRV or stress hormones during night sleep. After awakening, the standard deviation of RRI (84 +/- 31 vs 116 +/- 41 ms, P < 0.05) and low-frequency power of RRI (2153 +/- 2232 vs 4286 +/- 2904 ms, P < 0.05) were lower in OA than in CA. From sleep to after awakening, the coefficient of variation of RRI decreased more in OA than in CA (from 11.8 +/- 3.3 to 7.7 +/- 2.5%, P < 0.001 vs from 11.9 +/- 1.8 to 10.0 +/- 2.5%, P < 0.01, respectively, interaction P < 0.05). CONCLUSION The present findings suggest that in OA, cardiac autonomic modulation is at the level of control athletes during sleep, but the parasympathetic cardiac modulation is slightly diminished after awakening. Further investigations should concentrate on autonomic responses to different challenges, such as awakening in the present study.
Legume lectins — a large family of homologous proteins

More than 70 lectins from leguminous plants belonging to different suborders and tribes have been isolated, mostly from seeds, and characterized to varying degrees. Although they differ in their carbohydrate specificities, they resemble each other in their physicochemical properties. They usually consist of two or four subunits (25‐30 kDa), each with one carbohydrate binding site. Interaction with carbohydrates requires tightly bound Ca2+ and Mn2+ (or another transition metal). The primary sequences of more than 15 legume lectins have been established by chemical or molecular genetic techniques. They exhibit remarkable homologies, with a significant number of invariant amino acid residues, among them most of those involved in metal binding. The 3‐dimensional structures of the legume lectins are similar, too, and are characterized by a high content of β‐sheets and a lack of α‐helix. The location of the metal and carbohydrate binding sites, established unequivocally in concanavalin A by high resolution X‐ray crystallography, appears to be the same in the other legume lectins. Several of the lectin genes have been cloned and expressed in heterologous systems. This opens the way for the application of molecular genetics to the investigation of the atomic structure of the carbohydrate binding sites of the lectins, and of the relationship between their structure and biological activity. The new approaches may also provide information on the mechanisms that control gene expression in plants and on the role of lectins in nature. — Sharon, N. ; Lis, H. Legume lectins: a large family of homologous proteins. FASEB J. 4: 3198‐3208; 1990.
Ribonuclease-Gold Labels Heparin in Human Mast Cell Granules: New Use for an Ultrastructural Enzyme Affinity Technique

We evaluated an enzyme affinity-gold ultrastructural technique designed to identify RNA-rich structures, based on an RNase-gold (R-G) probe in human mast cells (HMCs). As expected, the R-G technique labeled RNA-containing ribosomes and nucleoli in HMCs. The heparin-rich secretory granules in HMCs were also labeled. Extensive studies revealed that HMCs isolated from lung or skin and sustained in short-term cultures, derived de novo in growth factor-supplemented cord blood cell cultures, or present in vivo in multiple sites all shared this property. We performed a large number of controls designed to examine the HMC granule binding characteristics of gold alone, of irrelevant protein- or enzyme-gold reagents, of the role of charge and enzyme activity after various enzyme digestions, after blocking with macromolecules, after exposure to inhibitors of RNase, of heparin, or to irrelevant enzyme inhibitors, including staining of macromolecule-containing test agar blocks and a variety of combined absorption and digestion experiments of the binding of R-G to HMC granules. These studies established that the R-G method detected heparin in this site in conventionally prepared, well-preserved electron microscopic samples. These findings demonstrate a new use for this enzyme affinity-gold technique in mast cell biology, based on the known property of heparin as an inhibitor of RNase.
Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis

Significance Saffron is a triploid, sterile species whose red stigmas constitute the most expensive spice on Earth. The color, the taste, and the aroma of the spice are owed to the crocus-specific apocarotenoid accumulation of crocetin/crocins, picrocrocin, and safranal. Through deep transcriptome analysis, we identified a novel carotenoid cleavage dioxygenase (CCD) whose expression profile parallels the production of crocetin. Using in bacterio, in vitro, and in planta functional assays, we demonstrate that CCD2 is the dioxygenase catalyzing the first dedicated step in saffron crocetin biosynthesis starting from the carotenoid zeaxanthin. Crocus sativus stigmas are the source of the saffron spice and accumulate the apocarotenoids crocetin, crocins, picrocrocin, and safranal, responsible for its color, taste, and aroma. Through deep transcriptome sequencing, we identified a novel dioxygenase, carotenoid cleavage dioxygenase 2 (CCD2), expressed early during stigma development and closely related to, but distinct from, the CCD1 dioxygenase family. CCD2 is the only identified member of a novel CCD clade, presents the structural features of a bona fide CCD, and is able to cleave zeaxanthin, the presumed precursor of saffron apocarotenoids, both in Escherichia coli and in maize endosperm. The cleavage products, identified through high-resolution mass spectrometry and comigration with authentic standards, are crocetin dialdehyde and crocetin, respectively. In vitro assays show that CCD2 cleaves sequentially the 7,8 and 7′,8′ double bonds adjacent to a 3-OH-β-ionone ring and that the conversion of zeaxanthin to crocetin dialdehyde proceeds via the C30 intermediate 3-OH-β-apo-8′-carotenal. In contrast, zeaxanthin cleavage dioxygenase (ZCD), an enzyme previously claimed to mediate crocetin formation, did not cleave zeaxanthin or 3-OH-β-apo-8′-carotenal in the test systems used. Sequence comparison and structure prediction suggest that ZCD is an N-truncated CCD4 form, lacking one blade of the β-propeller structure conserved in all CCDs. These results constitute strong evidence that CCD2 catalyzes the first dedicated step in crocin biosynthesis. Similar to CCD1, CCD2 has a cytoplasmic localization, suggesting that it may cleave carotenoids localized in the chromoplast outer envelope.
Photobactericidal porphyrin-cellulose nanocrystals: synthesis, characterization, and antimicrobial properties.

Adherence and survival of pathogenic bacteria on surfaces leading to concomitant transmission to new hosts significantly contributes to the proliferation of pathogens, which in turn considerably increases the threat to human health, particularly by antibiotic-resistant bacteria. Consequently, more research into effective surface disinfection and alternative materials (fabrics, plastics, or coatings) with antimicrobial and other bioactive characteristics is desirable. This report describes the synthesis and characterization of cellulose nanocrystals that were surface-modified with a cationic porphyrin. The porphyrin was appended onto the cellulose surface via the Cu(I)-catalyzed Huisgen-Meldal-Sharpless 1,3-dipolar cycloaddition having occurred between azide groups on the cellulosic surface and porphyrinic alkynes. The resulting, generally insoluble, crystalline material, CNC-Por (5), was characterized by infrared and diffusion (1)H NMR spectroscopies, gel permeation chromatography, and thermogravimetric analysis. Although only suspended, and not dissolved, in an aqueous system, CNC-Por (5) showed excellent efficacy toward the photodynamic inactivation of Mycobacterium smegmatis and Staphylococcus aureus , albeit only slight activity against Escherichia coli . The synthesis, properties, and activity of CNC-Por (5) described herein serve as a benchmark toward our overall objectives of developing novel, potent, bioactive, photobactericidal materials that are effective against a range of bacteria, with potential utilization in the health care and food preparation industries.
Formation of an Active Tissue-Specific Chromatin Domain Initiated by Epigenetic Marking at the Embryonic Stem Cell Stage

ABSTRACT The differentiation potential of stem cells is determined by the ability of these cells to establish and maintain developmentally regulated gene expression programs that are specific to different lineages. Although transcriptionally potentiated epigenetic states of genes have been described for haematopoietic progenitors, the developmental stage at which the formation of lineage-specific gene expression domains is initiated remains unclear. In this study, we show that an intergenic cis-acting element in the mouse λ5-VpreB1 locus is marked by histone H3 acetylation and histone H3 lysine 4 methylation at a discrete site in embryonic stem (ES) cells. The epigenetic modifications spread from this site toward the VpreB1 and λ5 genes at later stages of B-cell development, and a large, active chromatin domain is established in pre-B cells when the genes are fully expressed. In early B-cell progenitors, the binding of haematopoietic factor PU.1 coincides with the expansion of the marked region, and the region becomes a center for the recruitment of general transcription factors and RNA polymerase II. In pre-B cells, E2A also binds to the locus, and general transcription factors are distributed across the active domain, including the gene promoters and the intergenic region. These results suggest that localized epigenetic marking is important for establishing the transcriptional competence of the λ5 and VpreB1 genes as early as the pluripotent ES cell stage.
Conflicting Climate Change Frames in a Global Field of Media Discourse

Reducing global emissions will require a global cosmopolitan culture built from detailed attention to conflicting national climate change frames (interpretations) in media discourse. The authors analyze the global field of media climate change discourse using 17 diverse cases and 131 frames. They find four main conflicting dimensions of difference: validity of climate science, scale of ecological risk, scale of climate politics, and support for mitigation policy. These dimensions yield four clusters of cases producing a fractured global field. Positive values on the dimensions show modest association with emissions reductions. Data-mining media research is needed to determine trends in this global field.
Efficient MIMO Receiving Technique in IEEE 802.11n System for Enhanced Services

A combining of MIMO signal processing with OFDM is regarded as a promising solution of enhancing the performance of next generation WLAN system. Therefore, in this paper, IEEE 802.1 In system employing layered space-time architecture is considered for a high-rate transmission service. However, the IEEE 802.1 In system with V-BLAST scheme has not reached the available capacities due to imperfect nulling and cancellation. In this paper, efficient detection is proposed for IEEE 802.11 n system. In this proposed scheme, T probable streams are detected according to the first detected sub-stream of DFE detector and most probable stream is selected by likelihood test, since the performance of V-BLAST system depends on the first sub-stream detection capability. It has been shown that the performance of IEEE 802.1 In system can be improved by adopting the proposed detection, and the decoding complexity and system performance can be controlled by adjusting the number of T1.