Miracle-dz/UniFAR-FTUs huggingface dataset:
Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules

Antibodies raised against the Alcaligenes eutrophus poly-beta-hydroxybutyrate (PHB) synthase polypeptide were used for immunocytochemical localization of the synthase enzyme in whole cells and purified PHB granules. The data presented demonstrate for the first time that the synthase enzyme is located on the surface of the PHB granule rather than being incorporated inside the granule during its formation. From these basic observations and data from the recent literature, a model of granule assembly is proposed.
Research Note - The Impact of Community Commitment on Participation in Online Communities

Online discussion communities have become a widely used medium for interaction, enabling conversations across a broad range of topics and contexts. Their success, however, depends on participants' willingness to invest their time and attention in the absence of formal role and control structures. Why, then, would individuals choose to return repeatedly to a particular community and engage in the various behaviors that are necessary to keep conversation within the community going? Some studies of online communities argue that individuals are driven by self-interest, while others emphasize more altruistic motivations. To get beyond these inconsistent explanations, we offer a model that brings dissimilar rationales into a single conceptual framework and shows the validity of each rationale in explaining different online behaviors. Drawing on typologies of organizational commitment, we argue that members may have psychological bonds to a particular online community based on (a) need, (b) affect, and/or (c) obligation. We develop hypotheses that explain how each form of commitment to a community affects the likelihood that a member will engage in particular behaviors (reading threads, posting replies, moderating the discussion). Our results indicate that each form of community commitment has a unique impact on each behavior, with need-based commitment predicting thread reading, affect-based commitment predicting reply posting and moderating behaviors, and obligation-based commitment predicting only moderating behavior. Researchers seeking to understand how discussion-based communities function will benefit from this more precise theorizing of how each form of member commitment relates to different kinds of online behaviors. Community managers who seek to encourage particular behaviors may use our results to target the underlying form of commitment most likely to encourage the activities they wish to promote.
What Can I Do With a Patient With Diabetes and Critically Impaired Limb Perfusion Who Cannot Be Revascularized?

A patient with limb-threatening diabetic foot syndrome in whom relevant peripheral arterial occlusive disease is proven should receive arterial revascularization as soon as possible to avoid major amputation. However, 3 conditions may make it impossible to effectively restore limb perfusion: the patient will not profit from arterial revascularization owing to excessive tissue loss or sepsis (“too late”), the patient cannot be revascularized due to severe comorbidities (“too sick”), and arterial reconstruction is impossible because of technical and anatomical shortcomings in a patient who is otherwise fit for operation (“nonreconstructible limb perfusion”). This review outlines the therapeutic options specifically in the third group of patients in whom no technical option for surgical or endovascular revascularization exists.
Modeling and Simulation Investigation of Sensitivity of Symmetric Split Gate Junctionless FET for Biosensing Application

In this paper, an analytical model has been developed for a split gate junctionless (JL) MOSFET, which works as a bio-transistor to detect the analytes (biomolecules), such as protein, DNA, enzyme, cell, and so on, using the dielectric-modulation technique. We have also studied the neutralization of charged analytes when charge reduction due to drying out. The analytical model has been investigated by solving 2-D Poisson’s equation. For the validation of analytical results, a split gate JL MOSFET has been virtually fabricated through sprocess and the electrostatic properties of device simulated by sdevice tools of the “Sentaurus” device simulator. For the bio-species immobilization, an underlap region also known as open cavity is formed in the device through etching oxide material and gate material from the middle of the channel. The electrostatic properties of device, such as surface potential, threshold voltage, drain current, and sensitivity, get affected by the immobilization of analytes at the SiO2 layer (which acts as an adhesion layer in the cavity region). The change in the threshold voltage and transfer characteristics of the device is pondered as the sensing metric to detect the bio-targets under dry environment.
Morphology of diesel soot residuals from supercooled water droplets and ice crystals: implications for optical properties

Freshly emitted soot particles are fractal-like aggregates, but atmospheric processes often transform their morphology. Morphology of soot particles plays an important role in determining their optical properties, life cycle and hence their effect on Earth’s radiative balance. However, little is known about the morphology of soot particles that participated in cold cloud processes. Here we report results from laboratory experiments that simulate cold cloud processing of diesel soot particles by allowing them to form supercooled droplets and ice crystals at −20 and −40 °C, respectively. Electron microscopy revealed that soot residuals from ice crystals were more compact (roundness ∼0.55) than those from supercooled droplets (roundness ∼0.45), while nascent soot particles were the least compact (roundness ∼0.41). Optical simulations using the discrete dipole approximation showed that the more compact structure enhances soot single scattering albedo by a factor up to 1.4, thereby reducing the top-of-the-atmosphere direct radiative forcing by ∼63%. These results underscore that climate models should consider the morphological evolution of soot particles due to cold cloud processing to improve the estimate of direct radiative forcing of soot.
On the nature of the interdependence of the adrenal cortex, nonspecific stress and nutrition in the regulation of nitrogen metabolism.

IT IS now widely recognized that the adrenal cortex is concerned in some fundamental way with the ability of the organism to respond to injury in a characteristic fashion. However, the precise mechanism by which the steroids of the adrenal cortex participate in the metabolic response to injury still remains speculative. Earlier studies in this area led to the concept which is still widely held that certain of the metabolic alterations after injury in the healthy organism are a direct consequence of hypersecretion of adrenal cortical steroids (Selye 1946). However, later work (see Engel, 1951a and b for a review of the literature) has cast doubt on such a simple causal relationship and relatively few investigators active in this field accept this concept any longer. The best evidence against it is found in the report of Ingle, Ward and Kuizenga (1947) who showed that the characteristic negative nitrogen balance which follows fractures in normal,
Seasonal changes in plasma adrenocorticotropic hormone and α-melanocyte-stimulating hormone in response to thyrotropin-releasing hormone in normal, aged horses.

BACKGROUND Results of diagnostic tests for equine pituitary pars intermedia dysfunction (PPID), including endogenous ACTH concentration and the overnight dexamethasone suppression test (DST), are affected by season. New and potentially more sensitive diagnostic tests for equine PPID, such as thyrotropin-releasing hormone (TRH)-stimulated ACTH response, have been developed, but have had limited evaluation of seasonality. OBJECTIVE Our purpose was to evaluate seasonal changes in plasma ACTH and alpha-melanocyte-stimulating hormone (α-MSH) responses to TRH administration. ANIMALS Nine, healthy, aged horses with normal DST results. METHODS Synthetic TRH (1 mg) was administered IV. Plasma ACTH and α-MSH concentrations were measured at 0, 5, 10, 15, 20, 25, 30, 45, 60, and 180 minutes. Testing was performed in February, July, August, September, October, and November. Mean TRH-stimulated ACTH and α-MSH concentrations were compared across months and time by repeated measures analysis of variance. Significance was set at the P < . 05 level. RESULTS Concentrations of ACTH and α-MSH significantly increased after TRH administration. Endogenous and TRH-stimulated ACTH and α-MSH concentrations were significantly different across months with higher concentrations in the summer and fall compared with February. CONCLUSIONS AND CLINICAL IMPORTANCE Plasma ACTH and α-MSH responses to TRH administration experience seasonal variation, with TRH-stimulated ACTH and α-MSH concentrations increasing from summer through fall. These results support previous evidence of a seasonal influence on the equine pituitary-adrenal axis. More research is warranted with a larger number of horses to determine if seasonal reference ranges for TRH stimulation testing need to be defined.
Anterior Cingulate and Motor Network Metabolic Impairment in Progressive Supranuclear Palsy

Progressive supranuclear palsy is the prototype of subcortical dementia. Using positron emission tomography and statistical parametric mapping, we compared the glucose metabolic pattern obtained in this subcortical dementia to that observed in elderly healthy controls and in Alzheimer's disease, the prototype of cortical dementia. Progressive supranuclear palsy was characterized by a relative decrease of metabolism in anterior cingulate, adjacent supplementary motor area, precentral cortex, middle prefrontal cortex, midbrain tegmentum, globus pallidus, and ventrolateral and dorsomedial nuclei of thalamus. The data in progressive supranuclear palsy highlight predominant metabolic impairment in brain structures engaged in response selection, in attention for action, and in motor networks.
Bistable resistive switching of a sputter-deposited Cr-doped SrZrO/sub 3/ memory film

Sputter-deposited Cr-doped SrZrO/sub 3/-based metal-insulator-metal structures exhibited bistable resistive reversible switching as observed under bias voltage and voltage pulse. The ratio of resistance of the two leakage states (high-H, low-L) was about five orders of magnitude. The conduction of the L-state satisfied Frenkel-Poole emission and that of the H-state followed ohmic mechanism, causing the resistance ratio to decrease with increasing bias voltage. The transition time of H- to L-state was five orders of magnitude higher than that of L- to H-state. The transition from H- to L-state was the restricted part for reversible switching operation. The difference in transition time of the two states should be related to the respective conduction mechanisms.
Effect of organizational culture on employee commitment in the Indian IT services sourcing industry

Purpose – As clients of India's IT services providers continue to complain about knowledge loss caused by high attrition rates in their offshore delivery factories, the linkages between organizational culture and commitment of the Indian employee base are of interest to researchers as well as practitioners. This paper seeks to address these issues.Design/methodology/approach – Data was collected in the first half of 2012 through the ICCA™ appraisal framework from 291 Indian IT executives and managers working for two IT services sourcing provider organizations in Pune and Bangalore, India. To analyse the data, descriptive and inferential statistics were used together with multiple regression and confirmatory factor analysis.Findings – Taken together, this research makes several contributions. First, the results of data analysis exhibit that, among the organizational culture dimensions, in‐group collectivism and performance orientation are the antecedents with the biggest effect on employee commitment. Othe.
Task Analysis Revisited

A random sample of 500 phlebotomy technicians certified by a national organization was queried regarding perceptions of importance of 53 specific practice-related tasks representative of various departmental areas. The sample was surveyed via a mail questionnaire. Role centrality was assessed by considering mean importance ratings and by applying the Rasch measurement model to assigned importance ratings. Approximately 36% of the questionnaires received by respondents were returned. The results revealed which tasks were fundamental to the phlebotomy technician scope of practice. To assess longitudinal change in core duties, task saliency was considered with respect to similar data collected a decade earlier. Task importance may be considered by agencies that educate, credential, or employ phlebotomy technicians in providing current job function descriptions. The longitudinal methodology employed may be applicable to other job roles for which the assessment of change is of interest.
Mutated NPM1 in patients with acute myeloid leukemia in remission and relapse

Abstract Patients with newly diagnosed AML (n = 360) including 137 (38%) with normal karyotype (NK) were evaluated. Overall, 60 (16.6%) patients, including 46 of the 137 (33.5%) NK patients, had NPM1 mutation at baseline. Thirty-nine patients (30 NK) had available NPM1 status at the time of complete remission (CR) and all (100%) were negative for mutated NPM1. Among the patients with mutated NPM1 at baseline, 10/39 overall (25%) and 7/30 NK (23%) patients relapsed. NPM1 status was available for eight patients (six with NK) at the time of relapse. Among them, 7/8 overall (87%) and 5/6 NK (83%) patients had mutated NPM1, while 1/8 overall (12%) and 1/6 NK (16%) patients remained NPM1 wild type. Among the 300 patients (including 91 with NK) with wild type NPM1 at diagnosis, none acquired a mutated NPM1 clone, either at CR or at relapse. We conclude that mutated NPM1 is a stable and reliable prognostic marker in AML and can be used to assess MRD.
Michael Young's The Rise of the Meritocracy: A Philosophical Critique

ABSTRACT This paper examines Michael Young's 1958 dystopia, The Rise of the Meritocracy. In this book, the word ‘meritocracy’ was coined and used in a pejorative sense. Today, however, meritocracy represents a positive ideal against which we measure the justice of our institutions. This paper argues that, when read in the twenty-first century, Young's dystopia does little to dislodge the implicit appeal of a meritocratic society. It examines the principles of education and administrative justice upon which meritocracy is based, suggesting that since 1958 those principles have changed. Young's warning no longer has any effect on us because the meritocratic system it warns us against has been transformed.
Bioactive phytochemicals in an aqueous extract of the leaves of Talinum triangulare

Abstract An aqueous leaf extract of Talinum triangulare was screened for the presence of bioactive molecules, using gas chromatography coupled with pulse and flame ionization detectors. It had high carotenoids; moderate benzoic acid derivatives, hydroxycinnamates and flavonoids; and low terpenes, alkaloids, phytosterols, allicins, glycosides, saponins, and lignans contents. Ten known carotenoids (mainly 50.42% carotene and 33.30% lycopene), nine benzoic acid derivatives (mainly 84.63% ferulic acid and 11.92% vanillic acid), and six hydroxycinnamates (55.44% p‐coumaric acid and 44.46% caffeic acid) were detected. Also detected were eight lignans (88.02% retusin) and thirty flavonoids (50.35% quercetin and 39.36% kaempferol). The medicinal properties of the major components of these phytochemical families that were detected in the aqueous extract of the leaves were discussed herein and proposed to be explored for their potential health benefits. The great number of potentially active biomolecules and their multifunctional properties make Talinum triangulare a ready source of health‐promoting substances.
Total Sacrectomy and Reconstruction for Huge Sacral Tumors

The authors carried out successful total sacrectomy in three cases, two with giant cell tumors and one with a chordoma. The anterior and posterior approach is feasible for resecting huge sacral tumors en bloc, but it is important to reconstruct the continuity between the pelvic ring and spinal column using spinal instrumentation and sacral rods or AO plates. As total sacrectomy is a large-scale, time-consuming, and collaborative operation, two or three teams should be used in relays. Both pelvic and spinal surgical techniques are required. Postoperatively the patient can stand within 3 to 6 months and well-planned rehabilitation allows ambulation. In spite of the serious structural and neurologic damage caused, total sacrectomy can be rewarding procedure in terms of improved morbidity and mortality.
Statin response and pharmacokinetics variants

Despite statin therapy being effective in the primary and secondary prevention of coronary heart disease, the benefit of treatment varies between individuals. Interindividual variations in pharmacokinetics play a central role in the cause of variability of drug disposition, and, in turn, the drug’s clinical efficacy. Exploring genetic variations that influence pharmacokinetics may lead clinicians to apply the most efficient and safe drug therapy. So far, variants in eight candidate genes related to pharmacokinetics of statins have been investigated as the potential determinant of drug responsiveness or adverse event risk. All reported data remains inconclusive, but it has been suggested that combined analysis of more than two different polymorphisms, or a combination of genetic association and studies using in vitro recombinant expression techniques, may be more informative in predicting the specific phenotype of a genetic variant. Future studies using these approaches could provide more striking evidence, which may be sufficient to justify genetic analysis regarding pharmacokinetic variants in the clinical practice of statin therapy.
A multi-channel filtering approach to texture segmentation

Multichannel filtering techniques are presented for obtaining both region- and edge-based segmentations of textured images. The channels are represented by a bank of even-symmetric Gabor filters that nearly uniformly covers the spatial-frequency domain. Feature images are obtained by subjecting each (selected) filtered image to a nonlinear transformation and computing a measure of energy around each pixel. Region-based segmentations are obtained by using a square-error clustering algorithm. Edge-based segmentations are obtained by applying an edge detector to each feature image and combining their magnitude responses. An integrated segmentation technique that combines the strengths of the previous two techniques while eliminating their weaknesses is proposed. The integrated approach is truly unsupervised, since it eliminates the need for knowing the exact number of texture categories in the image. <<ETX>>
Role of donor hemodynamic trajectory in determining graft survival in liver transplantation from donation after circulatory death donors

Donation after circulatory death (DCD) donors show heterogeneous hemodynamic trajectories following withdrawal of life support. Impact of hemodynamics in DCD liver transplant is unclear, and objective measures of graft viability would ease transplant surgeon decision making and inform safe expansion of the donor organ pool. This retrospective study tested whether hemodynamic trajectories were associated with transplant outcomes in DCD liver transplantation (n = 87). Using longitudinal clustering statistical techniques, we phenotyped DCD donors based on hemodynamic trajectory for both mean arterial pressure (MAP) and peripheral oxygen saturation (SpO2) following withdrawal of life support. Donors were categorized into 3 clusters: those who gradually decline after withdrawal of life support (cluster 1), those who maintain stable hemodynamics followed by rapid decline (cluster 2), and those who decline rapidly (cluster 3). Clustering outputs were used to compare characteristics and transplant outcomes. Cox proportional hazards modeling revealed hepatocellular carcinoma (hazard ratio [HR] = 2.53; P = 0.047), cold ischemia time (HR = 1.50 per hour; P = 0.027), and MAP cluster 1 were associated with increased risk of graft loss (HR = 3.13; P = 0.021), but not SpO2 cluster (P = 0.172) or donor warm ischemia time (DWIT; P = 0.154). Despite longer DWIT, MAP and SpO2 clusters 2 showed similar graft survival to MAP and SpO2 clusters 3, respectively. In conclusion, despite heterogeneity in hemodynamic trajectories, DCD donors can be categorized into 3 clinically meaningful subgroups that help predict graft prognosis. Further studies should confirm the utility of liver grafts from cluster 2. Liver Transplantation 22 1469–1481 2016 AASLD.
Survival of Tumor Megaprostheses Replacements about the Knee

Limb salvage surgery is an effective procedure with a low risk of tumor recurrence. In an attempt to define the incidence of implant failure at 2, 5, and 10 years postoperatively, we retrospectively reviewed implant survival in a group (n = 105) of pediatric (< 18 years of age) and adult patients who were treated with distal femoral and/or proximal tibial implants for extremity tumors. Issues regarding the timing of failure, reason for failure, and whether pediatric patients had higher rates of failure were posed as secondary questions. The median followup was 57 months (1-235 months). Thirty-two (32/105, 31%) patients had 42 implant failures. The mean prosthesis Kaplan-Meier survivorship of the index group was 84% at 2 years, 73% at 5 years, and 59% at 10 years. Forty-seven percent of all failures occurred within 2 years postoperatively, and 69% occurred within 5 years postoperatively. Pediatric patients had a higher failure rate than adults (42% versus 24%). Aseptic loosening was the most common reason for failure (n = 18/32; 56%). The incidence of failure in tumor megaprostheses is similar to early published literature and the incidence of these failures is highest within the first 3 years.Level of Evidence: Therapeutic study, level IV. See Guidelines for Authors for a complete description of levels of evidence.
NORMAL PRESSURE HYDROCEPHALUS

The pathological changes in the brains of seven patients who had been clinically diagnosed as normal pressure hydrocephalus (NPH) are described and the possible etiological mechanisms are discussed. The pathological findings in all cases consisted of demyelination akin to Binswanger's type of encephalopathy, especially in the frontal lobes. Arteriosclerosis accompanied by occasional organized thrombi and scattered microinfarcts in the periventricular white matter were seen. Focal leptomeningeal fibrosis, diminution of arachnoidal granulations, and non‐specific aging processes were noted. Among the above of particular interest, was the degeneration of both periventricular and deep white matters with microinfarcts, and moderate to severe arteriosclerosis. On the basis of these observations, we assume that the degeneration in the white matter is not merely a secondary change due to the result of enlargement of ventricle, but plays an important role in the development of NPH. The development of NPH requires not only the disturbance of cerebrospinal fluid, but also the pre‐or coexisting vulnerability in the white matter caused by variables such as ischemia, hypoxia, and trauma.