Συγκριτική φαρμακοκινητική και βιοδιαθεσιμότητα δύο σκευασμάτων τυλοσίνης σε κοτόπουλα μετά την από του στόματος χορήγηση

The pharmacokinetics and oral bioavailability of two tylosin formulations was carried out in broiler chickens according to a single dose, randomized, parallel design. The two formulations of tylosin (Tylosina® and Tylan®) were given orally at a dose level of 25 mg/kg b.w. after an overnight fasting (n=15 chicken/group). To calculate tylosin bioavailability, fifteen more chickens was assigned as group 3 and was given a single intravenous dose of tylosin (25 mg/kg b.w.). Serial blood samples were collected at different time points up to 24 hour post-drug administration. A high performance liquid chromatography (HPLC) method was used for the determination of tylosin concentrations in chicken plasma. The pharmacokinetics analysis of the data was performed using non-compartmental analysis based on statistical moment theory with the help of commercially available software (WinNonlin®, Pharsight Corporation, Cary, NC, USA). There were no significant differences in the Cmax (3.05±0.63, 2.63±0.74 μg/ml), tmax (2.36±0.42, 2.30±0.38 h), t1/2β (1.99±0.38, 2.67±0.60 h), AUC0-12h (6.11±0.97, 5.37±1.16 μg.h/ml), AUC0-∞ (6.38±0.94, 5.57±1.15 μg.h/ml), MRT (3.53±0.24, 3.67±0.32 h), ClB/F (90.59±13.81, 169.38±54.44 ml/ min/kg) and Vdz/F (16.85±4.74, 43.96±18.24 l/kg) between Tylosina ® and Tylan®, respectively. The calculated oral bioavailability (F) for Tylosina® and Tylan® were 40.56 and 35.41%, respectively. Moreover, the relative bioavailability of Comparative pharmacokinetics and bioavailability of two tylosin formulations in chickens after oral administration Ehab A. Abu-Basha1*, Ahmad F. Al-Shunnaq2, Ronette Gehring3 1Department of Veterinary Basic Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan 2Department of Food Chemistry & Mycotoxin, Jordan Food & Drug Administration, Amman, Jordan 3Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA. Συγκριτική φαρμακοκινητική και βιοδιαθεσιμότητα δύο σκευασμάτων τυλοσίνης σε κοτόπουλα μετά την από του στόματος χορήγηση Ehab A. Abu-Basha1*, Ahmad F. Al-Shunnaq2, Ronette Gehring3 1 Τμήμα Κτηνιατρικών Επιστημών, Κτηνιατρική Σχολή, Πανεπιστήμιο Επιστήμης και Τεχνολογίας της Ιορδανίας, Irbid, Ιορδανία 2 Τμήμα Χημείας Τροφίμων & Μυκοτοξινών, Διοίκηση Φαρμάκων & Τροφίμων, Αμάν, Ιορδανία 3 Τμήμα Κλινικών Επιστημών, Κολέγιο Κτηνιατρικής, Πανεπιστήμιο του Κάνσας, Μανχάταν, Κάνσας, ΗΠΑ. J HELLENIC VET MED SOC 2012, 63(2): 159-166 ΠΕΚΕ 2012, 63(2): 159-166 Correspondence: Ehab A. Abu-Basha Department of Veterinary Basic Medical Sciences, Faculty of Veterinary Medicine Jordan University of Science and Technology P.O. Box 3030, Irbid 22110, Jordan, E-mail:abubasha@just.edu. jo Αλληλογραφία: Ehab A. Abu-Basha Tμήμα Κτηνιατρικών Επιστημών, Κτηνιατρική Σχολή Πανεπιστήμιο Επιστήμης και Τεχνολογίας της Ιορδανίας P.O. Box 3030, Irbid 22110, Ιορδανία, E-mail: abubasha@just.edu. jo Submission date: 22.03. 2012 Acceptance date: 25.06. 2012 Hμερομηνία υποβολής: 22.03. 2012 Ημερομηνία αποδοχής: 25.06. 2012 Υπεύθυνος επιμελητής σύνταξης: Α. Τυρπένου Original article Ερευνητικό άρθρο http://epublishing.ekt. gr | e-Publisher: EKT | Downloaded at 02/08/2019 14:01:03 | J HELLENIC VET MED SOC 2012, 63(2) ΠΕΚΕ 2012, 63(2) Tylosina® was 113.9% when compared to Tylan®. In conclusion, Tylosina® is comparable to Tylan® and both formulations can be used for treatment of susceptible microorganisms in veterinary medicine practice at a dose level of 25 mg/kg b.w.