Miracle-dz/UniFAR-FTUs huggingface dataset:
Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid

Agarose gel electrophoresis may be employed effectively for the detection and preliminary characterization of plasmid deoxyribonucleic acid (DNA) present in clinical isolates and laboratory strains of gram-negative microorganisms. The method is sensitive and does not require radioisotopes or ultracentrifugation. The estimation of plasmid mass from the extent of DNA migration in gels compares favorably with results obtained by electron microscopy of plasmid DNA purified by equilibrium density centrifugation. The method has proved to be a useful tool for survey work and the epidemiological investigation of plasmid dissemination, as well as an important adjunct to the genetic analysis of plasmids.
How Far Do Animals Go? Determinants of Day Range in Mammals

Day range (daily distance traveled) is an important measure for understanding relationships between animal distributions and food resources. However, our understanding of variation in day range across species is limited. Here we present a day range model and compare predictions against a comprehensive analysis of mammalian day range. As found in previous studies, day range scales near the \documentclass{aastex} \usepackage{amsbsy} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{bm} \usepackage{mathrsfs} \usepackage{pifont} \usepackage{stmaryrd} \usepackage{textcomp} \usepackage{portland,xspace} \usepackage{amsmath,amsxtra} \usepackage[OT2,OT1]{fontenc} \newcommand\cyr{ \renewcommand\rmdefault{wncyr} \renewcommand\sfdefault{wncyss} \renewcommand\encodingdefault{OT2} \normalfont \selectfont} \DeclareTextFontCommand{\textcyr}{\cyr} \pagestyle{empty} \DeclareMathSizes{10}{9}{7}{6} \begin{document} \landscape $$1/ 4$$ \end{document} power of body mass. Also, consistent with model predictions, taxonomic groups differ in the way day range scales with mass, associated with the most common diet types and foraging habitats. Faunivores have the longest day ranges and steepest body mass scaling. Frugivores and herbivores show intermediate and low scaling exponents, respectively. Day range in primates did not scale with mass, which may be consistent with the prediction that three‐dimensional foraging habitats lead to lower exponents. Day ranges increase with group size in carnivores but not in other taxonomic groups.
Discrete Evolutionary Birth-Death Processes and Their Large Population Limits

We study a class of discrete-state Markovian models of evolutionary population dynamics for k types of organisms, called discrete evolutionary birth-death processes (EBDP). The organisms in a model environment interact with each other by playing a certain game. Birth rates for type i organisms at time t are determined by their expected payoff in the game against an opponent chosen randomly from the environment at time t. Death rates at time t for all the organism types are equal, and proportional to the total population at time t. A discrete EBDP is therefore a continuous-time Markov chain on the nonnegative k-dimensional integer lattice, with state transitions to neighboring vertices only. A certain system of k nonlinear ordinary differential equations (ODE) can be derived from the discrete EBDP, and used as a deterministic approximation. We prove that a properly scaled sequence of EBDP's converges (in probability, uniformly on bounded sets) to the solution of the system of ODE's. We also prove that a different scaling of the sequence converges (in distribution) to a certain system of stochastic differential equations (SDE). An example based on reactive strategies for iterated prisoner's dilemma is used to illustrate population dynamics for discrete EBDP's, as well as the dynamics for the ODE and SDE limits.
Effect of different dosages of nitroglycerin infusion on arterial blood gas tensions in patients undergoing on- pump coronary artery bypass graft surgery

Background: On-pump coronary artery bypass graft (CABG) surgery impairs gas exchange in the early postoperative period. The main object on this study was evaluation of changes in arterial blood gas values in patients underwent on pump CABG surgery receiving different dose of intravenous nitroglycerin (NTG). Materials and Methods: sixty-seven consecutive patients undergoing elective on-pump CABG randomly enrolled into three groups receiving NTG 50 μg/min (Group N1, n =67), 100 μg/min (Group N2, n = 67), and 150 μg/min (Group N3, n = 67). Arterial blood gas (ABG) tensions were evaluated just before induction of anesthesia, during anesthesia, at the end of warming up period, and 6 h after admission to the intensive care unit. Results: Pao2 and PH had the highest value during surgery in Group N1, Group N2, and Group N3. No significant difference was noted in mean values of Pao2 and PH during surgery between three groups (P > 0.05). There was no significant difference in HCO3 values in different time intervals among three groups (P > 0.05). Conclusion: our results showed that infusing three different dosage of NTG (50, 100, and 150 μg/min) had no significant effect on ABG tensions in patients underwent on-pump CABG surgery.
Chlorine dioxide and chlorhexidine mouthrinses compared in a 3-day plaque accumulation model.

BACKGROUND The aim of this study was to investigate the inhibiting effect of a chlorine dioxide mouthrinse as opposed to a mouthrinse containing chlorhexidine (0.20%) during 3 days of plaque accumulation. METHODS At baseline, all participants (N=77) received a professional prophylaxis and were randomly assigned to the test (chlorine dioxide) or (positive) control (chlorhexidine) group. On the following 3 days, both groups rinsed twice daily for 1 minute with 10 ml test or control solution. At the end of the experimental period, plaque was assessed, and the panelists filled out a questionnaire. RESULTS Chlorhexidine inhibited plaque growth significantly more than the mouthrinse containing chlorine dioxide (plaque index=1.39 versus 1.96, respectively; P<0.001). The results of the questionnaire showed that the panelists found chlorhexidine easier to use and more effective. However, they preferred the taste of the chlorine dioxide mouthrinse and experienced less taste alterations. CONCLUSION Chlorine dioxide mouthrinse seems to be a less potent plaque inhibitor than chlorhexidine.
Selection by higher‐order effects of salinity and bacteria on early life‐stages of Western Baltic spring‐spawning herring

Habitat stratification by abiotic and biotic factors initiates divergence of populations and leads to ecological speciation. In contrast to fully marine waters, the Baltic Sea is stratified by a salinity gradient that strongly affects fish physiology, distribution, diversity and virulence of important marine pathogens. Animals thus face the challenge to simultaneously adapt to the concurrent salinity and cope with the selection imposed by the changing pathogenic virulence. Western Baltic spring‐spawning herring (Clupea harengus) migrate to spawning grounds characterized by different salinities to which herring are supposedly adapted. We hypothesized that herring populations do not only have to cope with different salinity levels but that they are simultaneously exposed to higher‐order effects that accompany the shifts in salinity, that is induced pathogenicity of Vibrio bacteria in lower saline waters. To experimentally evaluate this, adults of two populations were caught in their spawning grounds and fully reciprocally crossed within and between populations. Larvae were reared at three salinity levels, representing the spawning ground salinity of each of the two populations, or Atlantic salinity conditions resembling the phylogenetic origin of Clupea harengus. In addition, larvae were exposed to a Vibrio spp. infection. Life‐history traits and gene expression analysis served as response variables. Herring seem adapted to Baltic Sea conditions and cope better with low saline waters. However, upon a bacterial infection, herring larvae suffer more when kept at lower salinities implying reduced resistance against Vibrio or higher Vibrio virulence. In the context of recent climate change with less saline marine waters in the Baltic Sea, such interactions may constitute key future stressors.
Dapagliflozin – do we need it registered for type 2 diabetes?

Introduction: Inhibitors of the sodium–glucose co-transporter 2 (SGLT2) promote the excretion of glucose to reduce glycated hemoglobin (HbA1c) levels. Canagliflozin was the first SGLT2 inhibitor to be approved by the US FDA for use in the treatment of type 2 diabetes, and recently dapagliflozin has also been approved. Areas covered: We evaluated a recent Phase III clinical trial with dapagliflozin. Expert opinion: Dapagliflozin was studied as add-on therapy to sitagliptin with or without metformin, and was shown to lower HbA1c levels and body weight. The incidence of hypoglycaemia was low with dapagliflozin, but it did increase the incidence of urogenital infections. As no clear benefits have been identified for dapagliflozin over canagliflozin, which was the first gliflozin registered by the FDA, we do not fully understand why it was necessary to register dapagliflozin. Given that there are no completed cardiovascular/clinical outcome studies with dapagliflozin, and therefore no evidence of beneficial effect, it also seems premature to be using it extensively or considering it as an alternative to the clinically proven metformin.
Illness stories on the internet: what do breast cancer patients want at the end of treatment?

The study aims to elicit user requirements for internet‐based applications disclosing fellow patients' illness stories for the benefit of breast cancer patients. Twenty‐six breast cancer patients, recruited via the Dutch Patient Organization for Breast Cancer, were interviewed about their preferences with regards to content, appearance, and search options concerning fellow patients' illness stories online. The interviews were analysed quantitatively (SPSS) and qualitatively (NVivo). Participants were mainly interested in fellow patients' experiences about how to cope with emotions, the impact of cancer in daily life, and physical discomforts. Most participants preferred a section of an illness story in text format about a specific topic; some of them wanted to be able to click on to the corresponding complete story, comprising of text alone or supported by voice or video clip. A majority of participants wanted to be able to select illness stories on the basis of several authors' features, i.e. treatment underwent, age, presence of metastases, time since diagnosis, and whether or not caring for children. Participants gave arguments for their preferences. The findings of this study will be used for designing an online trial with breast cancer patients aiming at refining the user requirements. Copyright © 2007 John Wiley & Sons, Ltd.
The Effect of Magnesium Sulfate Therapy on Bleeding Time in Women with Threatened Preterm Labor

Magnesium sulfate is widely used in obstetrics and is drug of choice in two important complications of pregnancy, preeclampsia and preterm labor. The antagonistic effects of magnesium sulfate on calcium ion and on platelet aggregation may lead to changes in bleeding time. This study was conducted to evaluate the effect of magnesium sulfate on bleeding time in women with threatened preterm labor. A group of 40 patients with signs of preterm labor were treated with magnesium sulfate (4 grams in 200 ml D.W.5% in 20 minutes followed by maintenance dose of 2 grams per hour infusion) and template bleeding time (with IVY method), platelet count and mean arterial pressure were obtained before and 2 hours after magnesium sulfate infusion. The data were analyzed using paired t-test. The mean bleeding time before and after treatment were 161.1+50.6 and 169+61.8 seconds respectively and the mean platelet count was 179,925 and 185,250 respectively. The mean arterial pressure was 86.9+9 mmHg before treatment compared to 81.9+8.3 mmHg after treatment. According to the data presented in this study, there was no significant difference in bleeding time and platelet count before and after treatment with magnesium sulfate whereas the mean arterial pressure was significantly different (p<0.001) before and after treatment with magnesium sulfate.
RBM10 promotes transformation-associated processes in small cell lung cancer and is directly regulated by RBM5

Lung cancers are the leading cause of cancer-related deaths worldwide, with small cell lung cancer (SCLC) being the most aggressive type. At the time of diagnosis, SCLC has usually already metastasized, and an astonishing 95% of patients eventually succumb to the disease. This highlights the need for more effective SCLC screening and treatment options. Interestingly, the earliest and most frequent genetic alteration associated with lung cancers involves a lesion in the region to which the RNA binding protein RBM5 maps. We have recently shown that a decrease in RBM5 expression may be a key step in SCLC development, as RBM5 regulated many transformation-associated processes in SCLC cells. RBM5 is structurally and functionally similar to another RNA binding protein, RBM10. Both proteins have tumor-suppressor properties in a variety of cancer cell lines, and it has been suggested that RBM5 expression can influence RBM10. Due to their similarities, and the recent evidence that RBM10 is mutated in up to 21% of lung cancers, we hypothesized that RBM10 would share RBM5’s tumor-suppressor properties in SCLC. Using transcriptome analysis and functional assays, we show, however, that RBM10’s function was opposite to what we hypothesized; in the endogenously RBM5-null GLC20 SCLC cell line, RBM10 actually promoted cell proliferation and other transformation-associated processes. Using RNA immunoprecipitation followed by next generation sequencing (RIP-Seq) and Western blotting, we demonstrate that RBM5 post-transcriptionally regulated RBM10 expression via direct interaction with specific RBM10 splice variants. We propose a working model describing the impact of this interaction on cellular processes. Our results provide evidence that RBM10 expression, in RBM5-null tumors, may contribute to tumor growth and metastasis. Measurement of both RBM10 and RBM5 expression in clinical samples may therefore hold prognostic and/or potentially predictive value.
Evaluating Health Care

Evaluation of health care is equated with the intent to continuously improve rather than to control its quality. Quality is seen as being affected by and therefore embracing 1) patient's health status and attitudes on entry to care, 2) suitability of the delivery machinery (structure), 3) application of care (process), and 4) outcomes of application to care. It is suggested that analysis in depth at two points of this paradigm, the entry to care and the outcomes of care, are the most likely to reveal the basis of failures. These may largely reside with the patient, the health care delivery structure, or the inability of the patient and care deliverer to perform together appropriately. These suggest the kind of interventions most likely to provide good results in the future. Such interventions or changes are very likely to call for significant changes in many social institutions, including that of the health care delivery machinery. Focusing on process is seen as contributing to professional education but less likely to result in meaningful changes because it works on the assumption that what the professional in a given illness cycle offers is the main factor in achieving better quality care.
An Empirical Analysis of Technical Efficiency: The Role of IT Intensity and Competition

We analyze the impact of information technology (IT) on the technical efficiency of firms in the context of their observed competitive settings. Because competition can be a driver of efficiency and industries display varying degrees of competitiveness, firm-level efficiency is likely to display considerable heterogeneity. To shed light on these questions, we analyze the economic impact of IT on technical efficiency, a key component of efficiency, in heterogeneous competitive settings. Our study employs a number of econometric techniques, including a stochastic frontier and a generalized method of moments approach, on data from firms in a wide cross-section of industries. We find, after controlling for firm-level heterogeneity and potential endogeneity, that IT is positively associated with gains in technical efficiency but its impact is moderated by the degree of competition. Firms display large variation in their levels of technical efficiency partly because of the heterogeneous market competitiveness conditions they face. In more competitive industries, firms tend to deploy IT more intensively and use it more efficiently. Our study makes a distinct contribution relative to prior studies that have focused on the productivity impacts of IT while assuming perfect competition and not allowing for potential heterogeneity in firm-level efficiency. Overall, our results demonstrate that IT and competition are significant determinants of gains in technical efficiency and provide insight into how competition affects the returns to IT investment.
Exaggerated volume expansion natriuresis in rats preloaded with hypertonic saline: a paradoxical enhancement by inhibition of prostaglandin synthesis.

In preliminary experiments rats preinfused with hypertonic saline showed exaggerated natriuresis after an additional small volume expansion (SVE). This was systematically studied in anaesthetized Wistar rats prepared for clearance studies of the left kidney and measurements of medullary blood flow (MBF, laser-Doppler technique) and tissue electrical admittance (Y ), an index of interstitial ion concentration. The rats were preinfused i.v. with 3 mL of 5% NaCl during 90 min. A subsequent injection of isotonic saline, 0.5% of body weight, increased sodium excretion (UNaV ) from 2.1 +/- 0.5 to 4.5 +/- 1.1 micromol min-1 and urine flow (V ) from 12.0 +/- 2.3 to 24.3 +/- 5.6 microL min-1 (P < 0.02). The same volume of whole blood increased UNaV from 5.0 +/- 1.4 to 8.7 +/- 1.7 micromol min-1 and V from 22.3 +/- 5.1 to 37.4 +/- 5.9 microL min-1 (P < 0.01). The glomerular filtration rate, MBF and Y did not change. In rats preinfused with 0.9% saline no natriuresis was observed after SVE. To examine if prostaglandins (PG) were involved in SVE natriuresis, indomethacin (Indo), 5 mg kg-1 or sodium meclophenamate (Meclo), 7.5 mg kg-1, were added to the injected 0.9% saline. Paradoxically, both PG synthesis inhibitors enhanced natriuresis to SVE. After Indo UNaV increased from 2.0 +/- 0.6 to 7.6 +/- 1.3 micromol min-1, significantly more than after SVE alone (P < 0.001). At higher baseline UNaV, the increase with Meclo from 4.5 +/- 1.2 to 13.5 +/- 1.8 micromol min-1 was significantly higher than after whole blood infusion (P < 0.001). MBF decreased and Y increased after both inhibitors. Further studies are required to explain the enhancement of natriuresis after blockade of PG synthesis.
On the retrieval of sea ice thickness and snow depth using concurrent laser altimetry and L-band remote sensing data

Abstract. The accurate knowledge of sea ice parameters, including sea ice thickness and snow depth over the sea ice cover, is key to both climate studies and data assimilation in operational forecasts. Large-scale active and passive remote sensing is the basis for the estimation of these parameters. In traditional altimetry or the retrieval of snow depth with passive microwave remote sensing, although the sea ice thickness and the snow depth are closely related, the retrieval of one parameter is usually carried out under assumptions over the other. For example, climatological snow depth data or as derived from reanalyses contain large or unconstrained uncertainty, which result in large uncertainty in the derived sea ice thickness and volume. In this study, we explore the potential of combined retrieval of both sea ice thickness and snow depth using the concurrent active altimetry and passive microwave remote sensing of the sea ice cover. Specifically, laser altimetry and L-band passive remote sensing data are combined using two forward models: the L-band radiation model and the isostatic relationship based on buoyancy model. Since the laser altimetry usually features much higher spatial resolution than L-band data from the Soil Moisture Ocean Salinity (SMOS) satellite, there is potentially covariability between the observed snow freeboard by altimetry and the retrieval target of snow depth on the spatial scale of altimetry samples. Statistically significant correlation is discovered based on high-resolution observations from Operation IceBridge (OIB), and with a nonlinear fitting the covariability is incorporated in the retrieval algorithm. By using fitting parameters derived from large-scale surveys, the retrievability is greatly improved compared with the retrieval that assumes flat snow cover (i.e., no covariability). Verifications with OIB data show good match between the observed and the retrieved parameters, including both sea ice thickness and snow depth. With detailed analysis, we show that the error of the retrieval mainly arises from the difference between the modeled and the observed (SMOS) L-band brightness temperature (TB). The narrow swath and the limited coverage of the sea ice cover by altimetry is the potential source of error associated with the modeling of L-band TB and retrieval. The proposed retrieval methodology can be applied to the basin-scale retrieval of sea ice thickness and snow depth, using concurrent passive remote sensing and active laser altimetry based on satellites such as ICESat-2 and WCOM.
Mega-dose vitamin C attenuated lung inflammation in mouse asthma model

Asthma is a Th2-dependent disease mediated by IgE and Th2 cytokines, and asthmatic patients suffer from oxidative stresses from abnormal airway inflammation. Vitamin C is a micro-nutrient functioning as an antioxidant. When administered at a mega-dose, vitamin C has been reported to shift immune responses toward Th1. Thus, we tried to determine whether vitamin C exerted beneficial effects in asthma animal model. Asthma was induced in mice by sensitizing and challenging with ovalbumin. At the time of challenge, 3~5 mg of vitamin C was administered and the effects were evaluated. Vitamin C did not modulate Th1/Th2 balance in asthma model. However, it decreased airway hyperreactivity to methacholine, decreased inflammatory cell numbers in brochoalveolar lavage fluid, and moderate reduction of perivascular and peribronchiolar inflammatory cell infiltration. These results suggest that vitamin C administered at the time of antigen challenge exerted anti-inflammatory effects. Further studies based on chronic asthma model are needed to evaluate a long-term effect of vitamin C in asthma. In conclusion, even though vitamin C did not show any Th1/Th2 shifting effects in this experiment, it still exerted moderate anti-inflammatory effects. Considering other beneficial effects and inexpensiveness of vitamin C, mega-dose usage of vitamin C could be a potential supplementary modality for the management of asthma.