Miracle-dz/UniFAR-FTUs huggingface dataset:
Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein.

Primary rat embryo fibroblasts were transformed by a p53 mutant (alanine to valine change at amino acid 135) plus ras. This p53val135 mutant is temperature sensitive for a conformational change detected by the binding of a monoclonal antibody, PAb246, which recognizes the wild-type protein or the great majority of p53val135 at 32.5 degrees C. At 37 degrees C, both mutant and wild-type p53 conformational forms co-exist in the cells, while at 39.5 degrees C, the majority of the p53val135 in the cell is in a mutant conformation not recognized by PAb246 antibody. At 39.5 degrees C, the mutant p53 is localized in the cytoplasm of the cell. At 32.5 degrees C, the p53 protein enters the nucleus and stops the growth of these cells. At 37 degrees C where there is a mixture of mutant and wild-type p53, the wild-type p53 protein is in a complex with hsc70 and mutant p53 protein in the cytoplasm of the cell during G1. This wild-type protein enters the nucleus as the cells enter the S-phase of the cell cycle. At 32.5 degrees C, the cells stop replication and arrest at the G1/S border. After 48 hr at 32.5 degrees C, 91% of the cells are in the G1 fraction of the cell cycle. The S-phase cells appear to be immune to the p53 negative regulation of growth until they enter the next G1 period. (ABSTRACT TRUNCATED AT 250 WORDS)
Community pharmacies as sites of adult vaccination: A systematic review

ABSTRACT Vaccine-preventable deaths among adults remain a major public health concern, despite continued efforts to increase vaccination rates in this population. Alternative approaches to immunization delivery may help address under-vaccination among adults. This systematic review assesses the feasibility, acceptability, and effectiveness of community pharmacies as sites for adult vaccination. We searched 5 electronic databases (PubMed, EMBASE, Scopus, Cochrane, LILACS) for studies published prior to June 2016 and identified 47 relevant articles. We found that pharmacy-based immunization services (PBIS) have been facilitated by state regulatory changes and training programs that allow pharmacists to directly provide vaccinations. These services are widely accepted by both patients and pharmacy staff, and are capable of improving access and increasing vaccination rates. However, political and organizational barriers limit the feasibility and effectiveness of vaccine delivery in pharmacies. These studies provide evidence to inform policy and organizational efforts that promote the efficacy and sustainability of PBIS.
Effect of repeated infliximab therapy on serum matrix metalloproteinases and tissue inhibitors of metalloproteinases in patients with rheumatoid arthritis.

OBJECTIVE Matrix metalloproteinases (MMP) are involved in the articular tissue destruction processes in the pathogenesis of rheumatoid arthritis (RA). We investigated the effects of multiple infusions of infliximab, a chimeric anti-tumor necrosis factor-a (anti-TNF-a) antibody, on concentrations of serum MMP and tissue inhibitors of metalloproteinases (TIMP) in patients with active RA. METHODS Patients with RA were scheduled to receive 9 infusions of infliximab (3 mg/kg) at Weeks 0, 2, 6, and every 8 weeks thereafter. The therapy was combined with methotrexate (MTX) (7.5-20 mg/week). Serum concentrations of interstitial collagenase (MMP-1), stromelysin-1 (MMP-3), gelatinase B (MMP-9), TIMP-1, and TIMP-2 were measured by ELISA prior to infusion at Weeks 0, 2, 6, 14, 38, and 62. RESULTS The initial infusion of infliximab downregulated serum levels of MMP-1 (p < 0.001), MMP-3 (p < 0.001), MMP-9 (p < 0.001), TIMP-1 (p < 0.01), and TIMP-2 (p < 0.05). The second drug administration caused even more remarkable reduction of measured MMP (p < 0.001 in all cases) but not of TIMP levels. These changes were accompanied by decreased ratios of measured MMP to TIMP. Further infliximab therapy also significantly suppressed serum MMP levels, but was less effective. Before the first infliximab infusion serum concentrations of MMP and TIMP correlated with markers of RA activity such as the Disease Activity Score and C-reactive protein levels. After further drug administrations such associations, although less significant, were also noted. CONCLUSION Anti-TNF-a antibody therapy combined with MTX resulted in rapid clinical improvement and reduced serum MMP concentrations in patients with RA. Further infusions of infliximab maintained the decrease of MMP, although to a lesser extent than the first and second doses.
Biogenesis of structural intercellular junctions during cleavage in the mouse embryo

SUMMARY The preimplantation embryo differentiates the trophectoderm epithelium which, from the 32-cell stage, generates the blastocoel of the blastocyst and, after implantation, gives rise to most extraembryonic lineages of the conceptus. Trophectoderm differentiation begins at compaction (8-cell stage) when cell-cell adhesion, mediated by uvomorulin, and epithelial cell polarisation first occur. Here, we review our work on the biogenesis of tight junctions and desmosomes during epithelial differentiation. Tight junction construction begins at compaction and appears to be a gradual process, both at morphological and molecular levels. This maturation pattern may be due in part to sequential expression of tight junction constituents from the embryonic genome. Tight junction formation is dependent upon uvomorulin adhesion but can be inhibited by different means without apparently disturbing cell adhesion or polarisation. Cell interactions appear to regulate tight junction tissue specificity, in part by controlling the level of synthesis of constituents. Desmosome formation begins at the 32-cell stage, particularly as the embryo initiates blastocoel accumulation, and, in contrast with tight junction formation, does not appear to be a gradual process. Thus, nascent desmosomes appear mature in terms of their molecular composition. Desmosomal proteins are synthesised well in advance of desmosome formation but the synthesis of the principal glycoprotein components begins at the blastocyst stage and may regulate the timing of junction assembly. Implications of these differing patterns of biogenesis for the embryo are discussed.
FolM, A New Chromosomally Encoded Dihydrofolate Reductase in Escherichia coli

ABSTRACT Escherichia coli (thyA ΔfolA) mutants are viable and can grow in minimal medium when supplemented with thymidine alone. Here we present evidence from in vivo and in vitro studies that the ydgB gene determines an alternative dihydrofolate reductase that is related to the trypanosomatid pteridine reductases. We propose to rename this gene folM.
Acute accommodative and convergence insufficiency.

READING EASILY AND COMFORTABLY IS OF PARAMOUNT IMPORTANCE IN THE intellectual development of the child and young adult. It is a relative facility of accommodation and convergence that allows the young person to read at close range. When these mechanisms fail, reading becomes tiring, frequently producing headaches and even diplopia. Asthenopia in a school-age individual can be a significant handicap to learning. An inability to concentrate on written material creates frustration, impeding the learning process. Once the refractive error has been corrected and the symptoms persist, evaluation of the patient for mechanisms of binocular dysfunction is carried out. 1,2 Convergence insufficiency syndrome is defined in a variety of ways. This abnormality includes remote near-point of convergence and decreased amplitudes of convergence compared to divergence with poor recovery once fusion has been broken. These defects individually or in any combination, are enough to create symptoms.3-7 Over the past 4 years, we treated 26 young patients with asthenopic symptoms who had a combination of profoundly decreased accommodation and convergence in the absence of any other neurologic symptoms or signs. This retrospective study looked at this group to evaluate the relationship between abnormal accommodation and the findings of convergence insufficiency.
Competitions and incentives for smoking cessation.

BACKGROUND Material or financial incentives may be used in an attempt to reinforce behaviour change, including smoking cessation. They have been widely used in workplace smoking cessation programmes, and to a lesser extent within community programmes. Quit and Win contests are the subject of a companion review. OBJECTIVES To determine whether competitions and incentives lead to higher long-term quit rates. We also set out to examine the relationship between incentives and participation rates. SEARCH STRATEGY We searched the Cochrane Tobacco Addiction Group Specialized Register, with additional searches of MEDLINE (January 1966 to September 2004), EMBASE (1980 to 2004/8), CINAHL (1982 to 2004/8) and PsycINFO (1872 to 2004/6). Search terms included incentive*, competition*, contest*, reward*, prize*, contingent payment*, deposit contract*. SELECTION CRITERIA We considered randomized controlled trials, allocating individuals, workplaces, groups within workplaces, or communities to experimental or control conditions. We also considered controlled studies with baseline and post-intervention measures. DATA COLLECTION AND ANALYSIS Data were extracted by one author and checked by the second. We contacted study authors for additional data where necessary. The main outcome measure was abstinence from smoking for at least six months from the start of the intervention. We used the most rigorous definition of abstinence in each trial, and biochemically validated rates where available. Where possible we performed meta-analysis using a generic inverse variance model, grouped by timed endpoints, but not pooled across the subgroups. MAIN RESULTS Fifteen studies met our inclusion criteria. None of the studies demonstrated significantly higher quit rates for the incentives group than for the control group beyond the six-month assessment. There was no clear evidence that participants who committed their own money to the programme did better than those who did not, or that different types of incentives were more or less effective. There is some evidence that although cessation rates have not been shown to differ significantly, recruitment rates can be improved by rewarding participation, which may be expected to deliver higher absolute numbers of successful quitters. Cost effectiveness analysis is not appropriate to this review, since the efficacy of the intervention has not been demonstrated. AUTHORS' CONCLUSIONS Incentives and competitions do not appear to enhance long-term cessation rates, with early success tending to dissipate when the rewards are no longer offered. Rewarding participation and compliance in contests and cessation programmes may have more potential to deliver higher absolute numbers of quitters.
Do We Know What We Know? Investigating the Validity of Number Representation Indices

Predicting mathematical performance in individuals is critical in the development of evidencebased interventions. Thus, it is important to use valid measures when measuring mental number representation. Using symbolic and non-symbolic magnitude comparison tasks is the current method for measuring an individual’s mental representation of numbers. However, recent research questions the validity of the current indices of number representation (Inglis & Gilmore, 2014). This study examined the relations of the number representation indices in non-symbolic and symbolic number formats separately in adults and children. Participants for the current study include adults (n = 51) and senior kindergarten children (n = 159). The current study is an investigation of the number representation indices and their predictive relation with mathematical skill. Results showed three of five indices related across presentation formats for children, only overall response time related across formats for adults. Results suggest the related indices are more linked across presentation formats in children. Additionally, predictive validity of the indices is not shared across adults and children.
Hybrid Model for Trajectory Planning of Agile Autonomous Vehicles

This paper presents a hybrid architecture for autonomous trajectory planning of agile vehicles. A velocity control system providing the ability to accurately track trajectories is combined with a maneuver scheduler that enables execution of pre-programmed agile maneuvers. The closed-loop dynamics under this control architecture are described by a simple hybrid model, consisting of a set of constrained, linear time-invariant modes and discrete fixed-duration transitions in the state space. Given these dynamics, mixed integer linear programming (MILP) is used to compute optimal trajectories in cluttered environments. Continuous constraints and binary logic are combined to model the constraints governing the dynamics, to formulate switching rules between different velocity modes, to encode execution of agile maneuvers, and to account for obstacle avoidance. Both offline time-optimal planning and online receding horizon formulations are presented. The framework is applied in detail to a small-scale helicopter, for which several receding horizon results are given. A discussion about practical considerations regarding a real-time implementation concludes the paper.
Evaluating the impact of DevOps practice in Sri Lankan software development organizations

The purpose of this study is to conduct an analysis on practicing DevOps in software development companies in Sri Lanka. DevOps is extended from agile with a mix of patterns intended to improve collaboration between development and operations. The main objective of this research is to identify whether there is a relationship between quality, responsiveness to business needs and agility with implementation of DevOps. Other objectives of this research are to identify barriers Sri Lankan software companies have faced and provide recommendations to overcome those issues. This study was carried out using a deductive approach. Data have been collected from IT professionals in Sri Lanka who had prior experience or knowledge about DevOps. Recommendations are given based on interview feedback, hypotheses output and descriptive statistical analysis. According to results software professionals in Sri Lanka are in a perception that implementation of DevOps has a positive impact on Quality, Responsiveness to Business Needs and Agility to New Technologies. Culture, Automation, Measurement and Sharing are important factors to be considered when implement DevOps in Sri Lanka. As a result of this study it is recommended to implement and practice DevOps in Sri Lankan Software companies.
Rural–Urban Differences in the Prevalence of Chronic Disease in Northeast China

Rural–urban differences in the prevalence of chronic diseases in the adult population of northeast China are examined. The Jilin Provincial Chronic Disease Survey used personal interviews and physical measures to research the presence of a range of chronic diseases among a large sample of rural and urban provincial residents aged 18 to 79 years (N = 21 435). Logistic regression analyses were used. After adjusting for age and gender, rural residents had higher prevalence of hypertension, chronic ischemic heart disease, cerebrovascular disease, chronic low back pain, arthritis, chronic gastroenteritis/peptic ulcer, chronic cholecystitis/gallstones, and chronic lower respiratory disease. Low education, low income, and smoking increased the risk of chronic diseases in rural areas. Reducing rural–urban differences in chronic disease presents a formidable public health challenge for China. The solution requires focusing attention on issues endemic to rural areas such as poverty, lack of chronic disease knowledge, and the inequality in access to primary care.
Simplified characterization of Radar Cross Section of wind turbines in the air surveillance radars band

There is clear evidence that, above or within a region where wind farms are present, the normal functioning of Air Traffic Control (ATC) radars might be significantly impacted. In order to prevent these cases of impact, it is important to be able to identify the potential degradation of the service before it occurs. One of the most difficult tasks to achieve that goal is effectively characterizing the Radar Cross Section (RCS) of the wind turbines to be installed. In opposition to rigorous methods that can be computationally demanding, or constant representative RCS values than cannot accurately represent the real wind turbine models given the multitude of factors affecting RCS, in this paper a simplified RCS model to estimate the potential impact of wind turbines on Air Traffic Control radars is proposed. This model is validated against real measurements of RCS of a wind turbine, showing good agreement and highlighting the usefulness of the proposal.
Parvovirus B19 may have a role in the pathogenesis of juvenile idiopathic arthritis.

OBJECTIVE To determine the prevalence of human parvovirus B19 infection in patients with juvenile idiopathic arthritis (JIA) by detection of specific IgM, IgG, and viral DNA. METHODS Serum samples of 50 patients with diagnosis of JIA and 39 healthy controls were analyzed by ELISA to detect IgG and IgM anti-B19-specific antibodies. The parvovirus B19 genome was detected by nested polymerase chain reaction (PCR). The average age of the patients was 9.6 years (2-14 yrs); 30 were female (60%) and 20 male (40%). The definitive diagnoses of these patients corresponded to 19 systemic forms (38%), 11 to the oligoarticular variety (22%) and 20 to the polyarticular (40%). The average age of the control group was 7.8 years (2-16 yrs); the distribution by sex was 25 females (64%) and 14 males (36%). RESULTS IgM against parvovirus B19 was detected in 20% of the cases (10 patients) and B19 DNA genome by PCR in 48% (24 patients); in 10% of the cases (5 patients), both markers were detected. IgG was found in 32% (16 patients). In the control group neither IgM nor the viral genome was detected. However, 43.5% of the controls (17/39) had IgG against parvovirus B19, indicating past infection by the virus. CONCLUSION Our study confirms recent observations regarding a high prevalence of viral DNA in JIA patients and a possible role of this viral infection in JIA pathogenesis.
Procedure Skill Competence and Certification in Internal Medicine Residency Training

Background: Procedure skill training and competency certification are an important part of internal medicine residency training. Expert guidelines exist on the minimum number of procedures recommended to attain competence, but these have not been validated. Purpose: To study the validity of the American Board of Internal Medicine (ABIM) recommended procedure guidelines. Methods: We performed a retrospective review of all monthly procedure log sheets collected during residency training for the graduates of our internal medicine residency program. The number of procedure attempts and the length of time needed to be certified as competent in 13 different procedures were recorded and compared with ABIM guidelines. Results: There were 69 graduates with an average of 32.7 monthly procedure log sheets per resident. There was a wide variation in the number of attempts and time needed to attain competence in the measured procedures. Overall, our residents received their competency certification for each required procedure within 1 SD of the corresponding ABIM recommendation. Conclusions: Our study suggests that the ABIM recommended guidelines on procedure skill competence may be valid on the program level.
The small heat‐shock protein Hsp26 of Saccharomyces cerevisiae assembles into a high molecular weight aggregate

Hsp26 is one the major small heat‐shock proteins (Hsp) of the Saccharomyces cerevisiae. yet its cellular role remains to be discovered. To examine the cellular consequences of overexpression of Hsp26, the gene encoding this protein (HSP26) was overexpressed from a multicopy plasmid using either its own promoter or by coupling it to the effecient constitutive PGK promoter. The PGK promoter provided the opportunity to overexpress Hsp26 under nonstress conditions and such high level synthesis, prior to a lethal heat shock (50°C), gave a small but reproducible elevation in thermotolerance. In transformed strains overexpressing Hsp26 under either stressed or non‐stress conditions, the Hsp26 polypeptide was recovered almost exclusively as a high molecular weight aggregate. This high molecular weight aggregate (or heat‐shock granule, HSG) was purified by differential centrifugation and sucrose gradient density centrifugation and shown, by electron microscopic analysis, to be of a uniform size (15–25 nm diameter). Analysis of the purified HSG demonstrated that it had a molecular weight of 550 kDa, yet contained no other integral polypeptides or other macromolecules.
New Equivalent Circuit of Transformer Winding for the Calculation of Resonance Transients Considering Frequency-Dependent Losses

In this paper, a new equivalent circuit for the transformer winding is presented by which the eddy current losses in the transformer can be represented accurately at high frequencies. The new model enables representing the frequency-dependent impedances of a coil system using the idea of mutually coupled series Foster circuits. An effective method for the determination of model parameters is also provided which involves using the vector-fitting (VF) method for the rational approximation of the system impedance matrix using a certain number of common stable poles. A set of nonlinear equations is then established using the VF results that can be solved with the method of fixed-point iteration to obtain the parameters of the equivalent circuit in a fast and accurate way. The new model can be used for the calculation of resonance transients within the transformer, the situation wherein the maximal value of overvoltages is highly dependent to the damping effects or the loss phenomena in the transformer. Since the model only contains constant lumped parameters, it can be used for transient calculations in the time domain directly using the electromagnetic transients programs, such as the Alternative Transients Program or Electromagnetic Transients Program.
Potential Use in Forensics of a Novel Hybrid Gelatin—Dynamic Impact Assessment

Ballistic gelatin as simulant of the human body and organs is a support in forensics. After having obtained very good results for a new gelatin‐based composite in terms of physicochemical and rheological properties, this study focused on this material's mechanical behavior during stabbing and shooting versus bovine and porcine organs and standard ballistic gelatin. The hybrid gelatin has a predominantly elastic behavior at 23°C, whereas the elastic modulus becomes practically constant in the 10–0.1 Hz frequency range. In terms of stabbing behavior, the small variations obtained between porcine organs and surrogate are below 5%, the perforation indicating a good similarity. From the ballistic test results using 10 × 28T rubber balls, it has been seen that the hybrid ballistic gelatin conducts to more reliable and reproducible values of perforation/penetration versus standard gelatin, making from it a real candidate for use in forensic tests.